LOCUS       DASRWS010000001         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_132, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000001 DASRWS010000000
VERSION     DASRWS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFF72_00005"
     CDS             complement(<1..567)
                     /locus_tag="VFF72_00005"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZV91562.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVQNFPDVEVAAINDLLEPEYLAY
                     MLQYDSVHGRFKGSIAVDGQTLVVNGKRIRLTQERDPANLKWDEVGAAIVIESTGLFL
                     TRETARKHLDAGARKVILSAPSKDDTPMFVFGVNDRSYQGEAIISNASCTTNCLAPVA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDG"
     gene            complement(599..>2421)
                     /gene="tkt"
                     /locus_tag="VFF72_00010"
     CDS             complement(599..>2421)
                     /gene="tkt"
                     /locus_tag="VFF72_00010"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZV91563.1"
                     /translation="LDELKHFRKLHSKTPGHPEFGLTPGVETTTGPLGQGITNAVGMA
                     LAEKLLADHFNRAGHEVVNHHTYVFLGDGCLMEGISHEACALAGAWKLNKLIALYDDN
                     GISIDGAVKPWFIDDTPRRFESYGWNVIDGVDGHDVDAVDAAIARAKESSDRPTLVCC
                     KTVIGKGAPNRGGTAKAHGEALGAEEIRLTRQALGWAHAPFEIPDAAYAAWDAKARGA
                     AAEAAWRDQFDAYAAAEPARAAEYERRLAGELPDEWPARIAEAAASMHAKAETVASRK
                     ASQLVLETLTKVLPEMLGGSADLTGSNLTNTTSTPPLRFAADGAVTQDTEGRHGRHIN
                     YGVREFGMAAIMNGVALHDGLLPYGGTFLTFSDYSRNAIRMAALMKRRVVHVFTHDSI
                     GLGEDGPTHQSIEHAASLRLIPHLDVWRPCDTLETLAAWRAAVERRDGPSALLLSRQN
                     LPYAPRPGFDAARPDAMLETIGRGAYVLAEPSEVGLKGAARVVLIATGSEVQLALNAQ
                     QMLASAAHGKVAARVVSMPSTSLFDRQSAAWKSSVLPARIPRVAIEMGVTDGWWKYGC
                     AAVVGIDRYGESAPAADLFRHFGFTADNVVATVLKVLGRH"
BASE COUNT          342 a          810 c          854 g          415 t
ORIGIN      
        1 gccgtcgacg gttttctggg tggcggtggc cgcgtgcacg gtggtcatca ggccgcgctt
       61 gatgccccac ttgtcgttga gcaccttggc caccggggcg aggcaattgg tcgtgcacga
      121 ggcgttggag atgatcgcct cgccctggta gctcctgtcg ttgacgccga agacgaacat
      181 cggcgtgtcg tccttcgacg gtgccgacag gatcaccttc ctggcgccgg cgtcgaggtg
      241 cttgcgcgcc gtctccctgg tcaggaaaag ccccgtcgat tcgatgacga tcgcggcgcc
      301 gacctcgtcc cacttcaggt tcgccgggtc gcgctcctgc gtcaggcgga tgcgcttgcc
      361 gttgaccacg agcgtctggc cgtcgaccgc gatgctcccc ttgaagcggc cgtgcaccga
      421 gtcgtactgc agcatgtagg cgaggtactc gggctcgagc aggtcgttga tcgcggcgac
      481 ctcgacgtcg ggaaagttct ggaccgcggc gcggaacacc atgcgtccga tccggccgaa
      541 gccgttgatg ccgatcttga tggtcatgga aaagcgctcc tgcggttcga agaagaattc
      601 aatggcggcc gagcaccttc agcaccgtcg ccacgacgtt gtccgccgtg aagccgaagt
      661 gcctgaacag gtccgcggcg ggtgccgatt cgccgtagcg gtcgatcccc accactgcgg
      721 cgcagccgta tttccaccag ccgtcggtga cgcccatctc gatggcgacg cggggaatgc
      781 gcgccggcag caccgagctc ttccaggctg ccgactggcg gtcgaagaga gaggtcgacg
      841 gcatcgagac gacgcgggcc gccaccttgc cgtgcgcggc cgaggcgagc atctgctggg
      901 cgttgagcgc gagctgcacc tcggagccgg tggcgatcag gaccacgcgt gccgccccct
      961 tcaggcccac ctccgacggc tcggcgagca cataggcgcc acgcccgatc gtctcgagca
     1021 tggcgtcggg acgcgccgcg tcgaagccgg gcctgggcgc atagggcagg ttctgccgtg
     1081 aaaggaggag cgccgacggc ccgtcgcggc gctcgaccgc ggcgcgccag gcagccagcg
     1141 tctccagcgt gtcgcagggc cgccagacgt cgaggtgcgg aatcagtcgc agcgacgccg
     1201 cgtgctcgat cgactggtgc gtcgggccgt cctcgcccag tccgatggag tcgtgcgtga
     1261 agacgtggac gacgcgtcgc ttcatcagcg ccgccatgcg gatcgcgttg cggctgtagt
     1321 cgctgaaggt gaggaaggtg ccgccgtagg gcagcaggcc gtcgtgcagg gcgacgccgt
     1381 tcatgatcgc ggccatgccg aactcgcgca cgccgtagtt gatgtgccgg ccgtgccggc
     1441 cttcggtgtc ctgggtcacc gcgccatcgg cggcgaaacg cagcggcggc gtcgacgtgg
     1501 tgttcgtgag gttggagcct gtcaggtcgg cgctgccgcc gagcatctcg ggcaggacct
     1561 tcgtcagcgt ctcgagcacg agctggctcg ccttgcggct ggcgacggtc tcggccttgg
     1621 cgtgcatcga cgccgcggcc tcggcgatcc gcgccggcca ttcgtcgggc aactcgcctg
     1681 caaggcgtcg ttcgtactcc gcggcgcgcg ccggctcggc ggcggcatag gcatcgaact
     1741 gatcgcgcca ggccgcctcg gcggcggcgc cgcgggcctt cgcgtcccac gcggcatagg
     1801 ccgcgtcggg gatctcgaac ggcgcgtggg cccagccgag cgcctggcgg gtcaggcgaa
     1861 tctcctcggc gcccagcgcc tcgccgtgcg ccttggccgt gccgccgcga ttcggcgccc
     1921 ccttgccgat caccgtcttg cagcagacga gcgtcggccg gtcgctcgat tccttcgcgc
     1981 gcgcgatcgc cgcgtcgacg gcgtcgacgt cgtggccgtc gacgccgtcg atgacgttcc
     2041 agccgtacga ctcgaagcgc cgcggcgtgt cgtcgatgaa ccagggcttg accgcgccgt
     2101 cgatcgagat gccgttgtcg tcgtaaagcg cgatcagctt gttcagcttc caggccccgg
     2161 ccagggcgca ggcctcgtgg ctgatgcctt ccatcaggca gccgtcgccg aggaagacat
     2221 aggtgtgatg attcaccacc tcgtggccgg cgcgattgaa gtgatccgcg agcaactttt
     2281 cggcgagtgc catgcccact gcgttggtga tgccctgacc gagcggaccg gtggtggtct
     2341 ccacgcctgg cgtgagacca aactccgggt gacctggcgt cttgctatgt agtttgcgga
     2401 agtgcttcag ctcatcgagc g
//
LOCUS       DASRWS010000002         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_575729, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000002 DASRWS010000000
VERSION     DASRWS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575729"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VFF72_00015"
     CDS             complement(<1..165)
                     /locus_tag="VFF72_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HZV91564.1"
                     /translation="MRISTANSYDTGIATLMQRQLDMSNLQNQMTTGKRVNQASDDPA
                     AAAVAARAVAV"
     gene            complement(190..2181)
                     /gene="flgK"
                     /locus_tag="VFF72_00020"
     CDS             complement(190..2181)
                     /gene="flgK"
                     /locus_tag="VFF72_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZV91565.1"
                     /translation="MSVSSLLSIGSRAMAASYAQLTVTGNNIANANTPGYSQQTAHLE
                     TSFSQQTGSGFFGSGVDVATVTRAHSDFLTQEAATAASISAADDARSTQLQQLQNVFQ
                     TGQSGLGYVAQQAFNSFVDVANNPQDSSARQSALSSIGTLASNLSSASSQIDAQQAGI
                     ADQLRTSVASINTLTTQIANLNKQIASAQASGQPPNSLLDQRDTAINSLSQLAGVTTV
                     AAADGTVGVFLGGSQTLVLGSNASALVAVPDAFDPSRVQVGIKNGNTTTTFPDGFIAS
                     GSVSGLLRVQNHDIPDARGLLGQMAAAIAGAMNSQQALGLTQGTPASAGAPLLTTGAP
                     DVLPSSNNAMAGGVPVASYVNGSGTRVSSVGISVVDPKALQPSDYQLTADPSLPAGQY
                     KLTRLSDGTSQTVADGDVVDGFQIHVATPLPATRDSFLLRPVSNAASGMALALTDPSG
                     IAAASPVQAAMATGNAGTATVASLGVVDPSINPNLKATITFTDSSGDYNYSLVDTTGA
                     LPTVNGSGTVVAGQPIALNGWQLNLNGTPKSGDSVVVARTAYPAADNGNANAMLALGS
                     ANIVGLGGSVAAGQNVTDAYASALATVGVRVQSAMAAAAQSDSISTAATTAASNQSGV
                     NLDEEAARLIQYQQSYQAAAKMLQIAQTVFDSLLSIGSG"
     gene            complement(2228..>2389)
                     /locus_tag="VFF72_00025"
     CDS             complement(2228..>2389)
                     /locus_tag="VFF72_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HZV91566.1"
                     /translation="GVAAQAAQAPASAASASSFAQGLQRAGYATDPAYADKLARVINT
                     TLRVQKASA"
BASE COUNT          305 a          781 c          895 g          408 t
ORIGIN      
        1 cacggccacg gcccgcgccg cgaccgcggc ggccgccgga tcgtcgctcg cctggttgac
       61 gcgcttgccg gtcgtcatct ggttctgcag gttcgacatg tcgagctggc gctgcatcaa
      121 cgtggcgatg ccggtgtcgt aggaattggc ggtgctgatt cgcatggtgg ggcggcggga
      181 ggcggcgact cagccggagc cgatcgacaa caggctgtcg aacacggtct gggcgatctg
      241 cagcatcttg gctgcggcct ggtagctttg ctgatactgg atcaggcgcg ccgcttcctc
      301 gtcgaggttg acacccgact gattcgacgc cgccgtcgtc gctgcggtgg agatcgagtc
      361 ggactgcgcc gccgccgcca tcgcgctctg cacgcgcacg ccgaccgtgg cgagcgcgct
      421 ggcgtacgcg tcggtgacgt tctgtccggc cgcgacgctg ccgcccaggc cgacgatgtt
      481 cgccgagccg agcgccagca tggcgttggc attgccgttg tcagcggccg gatacgcggt
      541 cctggcgacg acgacggagt cgccgctttt cggcgtgccg ttcaggttca gctgccagcc
      601 gttgagggcg atcggctggc cggccacgac cgtgccgctg ccgttgacgg taggcaacgc
      661 gccggtggtg tcgacgaggc tgtagttgta gtcgccgctg ctgtcggtga aagtgatcgt
      721 cgccttcagg ttcggattga tcgacggatc gacgacgccg agcgaggcga ccgtcgcggt
      781 gccggcgttg cccgtggcca tggccgcctg caccggcgac gcggcggcga tgccgctcgg
      841 gtcggtgagc gccagggcca tgccgctcgc ggcgttcgac accgggcgca ggagaaagct
      901 gtcgcgcgtc gccggcaggg gcgtcgcgac gtggatctgg aagccgtcga cgacatcgcc
      961 gtcggcgacc gtctgcgacg tgccgtccga aagccgcgtc agcttgtact ggccggccgg
     1021 cagcgacgga tcggcggtca actggtagtc gctcggctgc agcgccttcg gatcgacgac
     1081 gctgatgccg acgctcgaga cgcgcgtgcc gctgccgttc acgtacgacg ccaccggcac
     1141 gccgccggcc atcgcgttgt tcgacgacgg cagcacgtcg ggcgcgccgg tcgtgagcag
     1201 cggcgctccg gccgacgccg gcgtgccctg ggtcaggccg agcgcctgct gcgaattcat
     1261 ggcgccggcg atcgcggccg ccatctggcc gagcaggccg cgggcgtcgg ggatgtcgtg
     1321 gttctgcacg cgcagcaggc cgctgaccga gccgctggcg atgaagccgt cggggaaggt
     1381 cgtcgtcgtg ttgccgttct tgatcccgac ctgcaccctc gacggatcga aggcatcggg
     1441 cacggccacc agcgcgctgg cgttgctgcc gagcaccaac gtctgcgaac cgccgaggaa
     1501 cacgccgacg gtgccgtcgg cagcggcgac ggtggtgacg ccggcgagct gcgacaggct
     1561 gttgatcgcc gtgtcgcgct ggtcgaggag gctgttcggc ggctgcccgc tcgcctgcgc
     1621 gctggcgatc tgcttgttga gattggcgat ctgcgtcgtc agggtgttga tcgacgcgac
     1681 cgacgtgcgc aactggtcgg cgatgcccgc ctgctgcgca tcgatctggc tcgacgcgct
     1741 gctgaggttg ctggcgagcg tgccgatgct cgacagggcc gactgccgcg ccgacgaatc
     1801 ctgcgggttg ttggcgacgt cgacgaagga gttgaaggcc tgctgcgcca cgtagccgag
     1861 gcccgactgt ccggtctgga agacgttttg cagttgctgc aactgcgtgc tgcgggcatc
     1921 gtcggcggcg gagatggaag ccgccgtcgc cgcctcctgg gtcaggaagt cgctgtgcgc
     1981 gcgcgtcacc gtcgccacgt cgacgccgct gccgaagaag cccgagccgg tctgctggct
     2041 gaacgacgtt tcgaggtgcg ccgtctgctg cgaatagccg ggcgtgttgg cgttggcaat
     2101 gttgttgccg gtgacggtga gctgggcgta gctcgccgcc atggcgcgcg agccgatgct
     2161 gagcagcgac gacacgctca tggctgggcc cccacgcttg ccgctgtcgc ggcgactttg
     2221 cgcccgctca tgccgaggcc ttctggacgc gcagcgtcgt gttgatgacg cgggcgagct
     2281 tgtcggcgta ggccggatcg gtggcgtagc cggcgcgttg caggccttgc gcgaacgagc
     2341 tcgccgacgc cgcgctggcc ggcgcctgcg ctgcctgcgc ggcaacgcc
//
LOCUS       DASRWS010000003         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2878157, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000003 DASRWS010000000
VERSION     DASRWS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2878157"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VFF72_00030"
     CDS             <1..1129
                     /locus_tag="VFF72_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91567.1"
                     /translation="ETCGKEHGCGLNERLGRELRWNGSAAATLLSGMSKSQKKFVEAV
                     AREKGLTIVGPEALTVRRQRCGIGFCFRLESGALLRDPREIARLKALAVPPAYSDVRY
                     AEDPSAHLQAVGTDAAGRLQYRYHPKWTEVREALKARRLSGLAKALPDIERAVAKALQ
                     AKEIDAGFASAAVVHLVSRTAIRAGGEAYAREHGTRGATTLLKSNVTLAEGLVTLQFK
                     AKGGKPVLKEVRDSRLRAALQALMALPGRRLFQYRDSEGTVHAIRAGDVNAYLCGVAG
                     RRLSLKDFRTLIASAAVLEALAATEPAPSERARRSQLRSVVTAVAEELANTPTVCRKS
                     YVHEAVVTAFEEGALARLRKPMRSPIKKAEVLARIVTRHGS"
     gene            complement(1142..1519)
                     /locus_tag="VFF72_00035"
     CDS             complement(1142..1519)
                     /locus_tag="VFF72_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91568.1"
                     /translation="MPVHWTISHPARLVIAVAKDDVKVEDIEQYFAGVTADGAMSYRK
                     IFEIGTTSMAMSDDMLRGLGQRVMLYAQHGQLGPLAIVASSDESYAQAQVFASSATAK
                     RSLQIFREMHAARRWLDEQEAPR"
     gene            complement(1535..1756)
                     /locus_tag="VFF72_00040"
     CDS             complement(1535..1756)
                     /locus_tag="VFF72_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91569.1"
                     /translation="MERGLLAPLSPNELTALRRVANGISKPKHLRAASVARLKLLALV
                     EEHEGSITLTPLGTKRCWADTGRSHGPQT"
     gene            1957..2202
                     /locus_tag="VFF72_00045"
     CDS             1957..2202
                     /locus_tag="VFF72_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91570.1"
                     /translation="MVITLVAFGAVGVLIGWRRGTKAIYRRSVSEPNLALGETRRDYD
                     RRLRRRRKIGRAVATLVYCLAGVGVGLLFVAATALRR"
     gene            2296..2541
                     /locus_tag="VFF72_00050"
     CDS             2296..2541
                     /locus_tag="VFF72_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91571.1"
                     /translation="MGLSAHAQSRRSSPEQQYCEALSDLYMRYVGNPETQPRNVRRND
                     ADAEKALSQCRHGDAAASIPVLERKLADNRITLPQRP"
     gene            complement(2566..>3084)
                     /locus_tag="VFF72_00055"
     CDS             complement(2566..>3084)
                     /locus_tag="VFF72_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HZV91572.1"
                     /translation="LTKLYYNVTPGTGITGTFDSLRVFFGGALSGVWHDGNWRFQPSI
                     LAAWGSETQNAYTDSNNTFVPAQTVTFGRIGAGPEIGYTFKDDRRGWSFEPFGLAKVN
                     VDFSSTPVYTFYGLTPVVARSGTQASGQVGGGIAMLLDEGFYLRLQASYDSIGVPGLD
                     IWSGRIRAGKTF"
BASE COUNT          514 a          983 c         1067 g          520 t
ORIGIN      
        1 cgaaacgtgc ggcaaagaac atggatgcgg gctcaacgaa cgcctgggcc gggagttgcg
       61 ctggaacggg tctgctgcgg caacgttgct ttccggcatg agcaagtccc agaagaaatt
      121 cgtcgaagcc gtcgctcgcg aaaaaggact gactatcgtc ggtcccgaag cgctgaccgt
      181 gcggcgccaa cgctgcggca tcggcttctg ctttcgcctc gaatcggggg cacttctgcg
      241 cgacccgcgc gagatcgccc gactgaaagc cctcgcggtc ccaccggcct attccgacgt
      301 gcgctatgcc gaggatccct cggcgcatct gcaggcagtg ggcaccgacg ccgccggccg
      361 gctgcagtac cgctaccatc cgaaatggac cgaggtgcgc gaggcgctga aggcacggcg
      421 actctcggga cttgccaagg cgctgcccga catcgagcgc gcggtagcga aggcactgca
      481 ggcgaaagag atcgatgccg gttttgcgtc ggcggcggtg gtgcatctcg tcagccgcac
      541 ggcgatccgc gccggcggcg aggcctatgc ccgcgagcac ggcacgcgcg gcgcgaccac
      601 gctgctcaag tcgaacgtga ccctggccga aggcctcgtg acgctgcagt tcaaggcgaa
      661 gggcggcaag ccggtcttga aggaggtgcg cgatagccgc ctacgcgcgg cgctgcaggc
      721 gctgatggcg ctgccgggcc gacggctgtt ccagtatcgc gatagcgaag gcacggtgca
      781 cgcaatacgc gccggcgacg tcaatgcgta tctgtgcggc gtcgcaggcc ggcgcttgtc
      841 gctgaaggac ttccgcacgc tgatcgcgtc ggcggccgtg ctcgaggcgc tggcggcgac
      901 cgagcccgcg cccagcgaac gtgcccgccg ctctcagttg cgcagcgtcg tgaccgcggt
      961 agcggaggaa ctcgccaaca cgccgaccgt gtgccgcaag agctacgtgc acgaggccgt
     1021 ggttaccgcc ttcgaggagg gcgcactcgc acgcctgcgt aagccgatgc gctcgccgat
     1081 caagaaggcc gaggtcctgg cgcgcatcgt cacgcgccac ggctcttaga cgaagtcgct
     1141 cctagcgcgg cgcttcctgc tcgtcgagcc agcgccgcgc cgcgtgcatc tcccggaaga
     1201 tctgcagcga tcgcttggcc gtggcggatg aggcgaagac ctgcgcttgc gcgtagctct
     1261 cgtcagagct ggcgacgatc gccagcgggc cgagctggcc gtgctgggcg tagagcatga
     1321 cgcgctggcc gaggcctcgc agcatgtcgt cgctcatcgc catcgacgtg gtgccgatct
     1381 cgaagatctt gcggtaggac atcgcgccgt cggcggtcac gccggcgaaa tactgctcga
     1441 tgtcctcgac cttcacgtcg tccttcgcga cggcaatcac caggcgggca gggtgggaga
     1501 tcgtccagtg aaccggcatt cggcgatgac cgtgtcaggt ctgcgggccg tgactgcggc
     1561 ccgtgtcggc ccagcagcgt tttgttccca ggggcgtgag cgtgatcgat ccctcatgct
     1621 cctccaccag ggcgagcaac ttcaaccgcg caacggaggc ggcacgcaaa tgcttgggct
     1681 tggaaatacc gttggctacc cgccgtagcg cggtcagttc attggggctg agcggggcaa
     1741 gcaggccacg ttccattggc acctccggct cggattggca tcaaagacct tggacccggg
     1801 gctggcaatg tccgaagtgt gaaattcgtg tggcttgcgt tacagcgaat gcagcagtgg
     1861 tgcgccgaca tttgctcgcg ctggcggtcg tgtaatgcgc tgcggtatac tgactgacag
     1921 cgagccgggg agggctctgg ggtgttcagt ccggcaatgg tgatcacgct ggtcgccttc
     1981 ggcgcggtcg gcgtgctgat aggttggcgc cgcggcacga aggcgatcta tcgccgctcg
     2041 gtcagcgagc ccaatctcgc gctcggtgag accaggcggg actacgatcg gcggttgcgc
     2101 cgccgacgca agatcggccg ggcggttgct acgcttgttt attgcctggc cggggtcggc
     2161 gtcggcctgt tgttcgtcgc tgcaacggcg ctgcgtcgtt agcaacgcgc ctgctgtcgc
     2221 gacgttgtcc taccgaagcg ccatgcgagg ttccgcgtga gcaaagttgt tcctgccgtc
     2281 gctatcgtct tcctggtggg actatcggcc cacgcgcaga gtcgccggtc gtcgcccgag
     2341 cagcagtact gcgaggcgct gagcgacctc tacatgagat acgttggcaa ccccgagacc
     2401 cagccgcgca acgttcggcg caacgacgcc gatgcagaga aggcactctc gcaatgccga
     2461 cacggcgacg ccgccgcgtc gatcccggtg ctggagcgca agctcgctga caacaggatc
     2521 acgttgcccc agcgtccgtg aggcgctggg tttcagctgc cgcggtcaga acgtcttgcc
     2581 ggcgcggatg cgacccgacc agatgtcgag accgggtacg ccaatcgaat cgtagctcgc
     2641 ctgcaggcgc aggtagaatc cttcgtcgag cagcattgcg atgccgccgc ccacctggcc
     2701 ggaagcctgc gtgcccgagc gcgcgaccac cggcgtcaga ccatagaagg tgtagacggg
     2761 cgtcgaggag aaatcgacgt tgaccttggc gagcccgaac ggctcgaagc tccagccgcg
     2821 gcgatcgtcc ttgaaggtgt agccgatctc cgggccggcg ccgatccgcc cgaaggtgac
     2881 ggtctgcgcc ggtacgaagg tgttgttgct gtcggtatag gcgttctgcg tctcgctgcc
     2941 ccacgccgcg aggatgctcg gctggaagcg ccagttgccg tcgtgccaga cgccgctgag
     3001 cgcgccgccg aagaagacac gcagcgagtc gaacgtaccg gtgataccgg tgccgggcgt
     3061 gacgttgtag tagagcttgg tcag
//
LOCUS       DASRWS010000004         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1439511, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000004 DASRWS010000000
VERSION     DASRWS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439511"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFF72_00060"
     CDS             complement(<1..1118)
                     /locus_tag="VFF72_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV91573.1"
                     /translation="MGQSVGDRGARNAMKGGSTLARPLMLTSMCIGFGVVQLDVTIVN
                     TALESIGSSFGGAISGLQWIVNTYTICFAALILTAGALGDRLGAKKVFVSGFGVFTAA
                     SLAAALAPSAALLVAARGLQGLGAALLVPNSLALLRHAYPDDKDRGRAVGIWAAGASL
                     ALTAGPVAGGGLIALVGWRSIFLVNVPLGLTGLWLAWRFARETPRAPGRRLDLPGQLA
                     AISALGCVAFALIEAGTSGWGSVRVVAGFAASAMATTLFVLQERRETQPMLPLSLFGQ
                     PLFTAATVVGVLANTAFYGLIFVFSLYFQEVQGLSALLTGVAFLPMIGMILPFNLLAA
                     PIAERIGGPTTIVVGLAISALGCTLLLGIGPDSRYASL"
     gene            complement(1257..2114)
                     /locus_tag="VFF72_00065"
     CDS             complement(1257..2114)
                     /locus_tag="VFF72_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV91574.1"
                     /translation="MTSARPPAPYTIRGIQEMLGLTRGVILGLVDSGFVRPSRGERNE
                     YRFTFRDIVVLRSAVELRAAGIAPRRILAALRTLKARLPDEMPVEGLRISAVGSDITV
                     REGGARWQADSGQLVMDFELAQAEGRSVAFLPLALPARPATSEPQESDGDAATWFARG
                     EALETSDRGAAEAAYRRALALDPAYADAYLNLGALLCEARRCAEAVTLYDRAVRRFPG
                     DALLHFNRAVALEDQGRPTEALAGYDTALRLDDALADAHYNIARLHEQLGDAKQAVRH
                     FSAYRRLQR"
BASE COUNT          344 a          730 c          778 g          263 t
ORIGIN      
        1 cacagcgacg cgtagcggct gtccggcccg atgccgagaa gcagcgtgca gcccagcgcc
       61 gagatcgcca ggccgaccac gatcgtcgtc gggccgccga tgcgctccgc gatcggcgcg
      121 gcgagcaggt tgaacgggag gatcattccg atcatcggca ggaaggccac tccggtcagg
      181 agcgcggaca agccctgcac ctcctggaag tagaggctga agacgaagat caggccgtag
      241 aaggcggtat tggcgagcac gccgacaacg gtcgcggcgg tgaacaacgg ctgtccgaag
      301 agcgacaggg gcagcatggg ctgcgtctcg cggcgctcct ggagcacgaa cagcgtcgtc
      361 gccatcgcag atgcggcgaa tcccgccacg acccggacac tgccccagcc ggaagtgccg
      421 gcctcgatca atgcgaaggc cacgcagccc agggcagaaa tggctgccag ctggccggga
      481 aggtcgagcc ttcggcctgg cgctcgcggt gtctcccgcg caaagcgcca ggcgagccag
      541 aggccggtca ggccgagcgg cacgttgacg aggaagatgc tgcgccagcc gacgagcgcg
      601 atcaggcctc cgccagcgac gggcccggcc gtgagcgcaa ggctcgcgcc ggcggcccag
      661 atgccgacgg cgcggccccg atccttgtcg tcgggatagg cgtggcgaag aagggcgagc
      721 gagttgggca cgagcagcgc cgcgccgagg ccctgcaggc ctctggcggc cacgagaagc
      781 gcggccgagg gcgccagcgc cgccgccagc gaggccgccg tgaagacgcc gaagccggac
      841 acgaagacct tcttcgcacc gagccggtcg cccagcgcac cggctgtcag gatgagcgcg
      901 gcgaagcaga tcgtgtacgt gttgacgatc cactggagtc cggagatcgc gccgccgaac
      961 gacgagccga tcgactcgag cgccgtgttg acgatcgtga cgtcgagctg cacgacacca
     1021 aagccgatgc acatgctcgt cagcatcagc ggccgcgcca gcgtcgatcc gcccttcatc
     1081 gcgttgcggg cgccacggtc gccgaccgac tgtcccacga gtcgcacgcg tttcccatgg
     1141 cgcagactct agcgtcgacc gcggcgacga cgattcggca cggaccgaat cgtcatgcgc
     1201 ggcaccgatc gacgatgtcg cggtcgcgcc actgcgggcg cggcccgctg ccgccctcag
     1261 cgctgcagcc gccggtaggc gctgaagtgg cgcaccgcct gcttggcgtc gccaagctgc
     1321 tcgtgcaggc gggcgatgtt gtagtgcgcg tcggcgagcg cgtcgtcgag gcgaagcgcc
     1381 gtgtcgtagc cggccagcgc ctctgtcggt cgcccctgat cctcgagcgc gacggcgcgg
     1441 ttgaagtgaa gcagcgcgtc gcccgggaag cggcgaacgg cgcggtcgta gagcgtcacc
     1501 gcctcggcgc agcgacgcgc ctcgcagagc agcgcgccga ggttcaggta ggcgtcggca
     1561 taggccgggt ccagcgccag cgcccggcga taggcggcct cggcagcgcc gcggtcgctc
     1621 gtctcgagcg cctcgccccg cgcgaaccac gtcgcggcgt cgccgtcgct ttcttgcggc
     1681 tcggaggtgg ccgggcgagc cggcaaggcg agaggcagga atgcgacgct gcggccctcg
     1741 gcctgcgcca gctcgaaatc catcacgagc tggcccgagt cggcctgcca gcgggcaccg
     1801 ccctcgcgca cggtgatgtc gctgccgacg gcgctgatgc gcagcccttc gacgggcatt
     1861 tcgtcaggca ggcgcgcctt cagcgtccgc agcgcggcga ggatgcggcg cggtgcgatg
     1921 ccggcggcgc gcagctcgac ggcgctgcgc aggacgacga tgtcgcgaaa ggtgaaccgg
     1981 tattcgttgc gctcgccccg gctcgggcgg acgaagccgg agtcgaccag gccgaggatg
     2041 acgccgcgcg tcaggccgag catctcctgg atgccgcgga tcgtgtacgg cgcaggcggc
     2101 ctggcgctcg tcact
//
LOCUS       DASRWS010000005         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4030991, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000005 DASRWS010000000
VERSION     DASRWS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4030991"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFF72_00070"
     CDS             <1..897
                     /locus_tag="VFF72_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91575.1"
                     /translation="HFDRVEWLYLPDSNSSTAALKNGEVDMIEQVPPDYITPLRSDPN
                     ITVGSGGSYQGFIVFNQLFPPFDNPKVRQAVLHAVNQEKFVAALGYPLDMRMTYCATW
                     FICGGPNQTEAGAEPFRKVDVAKAKQLLAEGGYKGEKVVVLVPTDITYLNAGALVTVQ
                     TLKSIGMNVDAQTSDWATITARRAKKSAPDAGGWNVYVTVAGEFDVNSPMTNAYLSAA
                     CGNSLPGWPCDKTLDEIRTAWVRETVPAKRKELLDRFQERAFQAVPYAYFGQYSPAYA
                     ARKSIKHLDEYWGIPTLWALDK"
     gene            940..1878
                     /locus_tag="VFF72_00075"
     CDS             940..1878
                     /locus_tag="VFF72_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV91576.1"
                     /translation="MGMILRRVAATLPVMAIVALLVFLLIHLAPGDPAALIAGDLAST
                     DDIARLHTSLGLDQPLWRQFALWAGHLARGDLGTSIFTQVPVAELLAQRLEPTLSIAV
                     LTILISIVVAIPLGTLAAYRAGSWIDRAAMLFAVLAFSVPVFLVGYLLIWGFAIRLHW
                     LPVQGYARLGDGVGAWFSHLVLPCVNLALVYIALLTRMTRATVIEVLQEDYIRTARAK
                     GLGVLAVLDHALRNAAIPIATTVGVGIALLIGGVVVTETVFAIPGVGRLVVESVQRHD
                     YPVIQSVLLLSAGAYVLVNLLIDLSYGLFDPRIRDA"
     gene            1916..2791
                     /locus_tag="VFF72_00080"
     CDS             1916..2791
                     /locus_tag="VFF72_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV91577.1"
                     /translation="MAAVATPPSARRGPWRRPLAWLLRHPTLAFGALLLIAMAAIAIA
                     APWLATHDPDDIDPLARLQPPTAEHRFGTDALGRDVYSRTLLGARVSLAVGVAVAAIS
                     ATIGIVLGLVAGFVRPLEGVLMRTMDGLMAIPGILLAIAAMAVVRASLATVIVAITIP
                     EVPRVVRLVRSLALTLREQLYVEAARAVGTRLPAILWRHVLPNMMAPLIVQATFVAAS
                     AVLIEAALSFLGVGVPGQTPSWGNMMAEGRNFVAVAFHIILYPGLFLATTVLAINLLG
                     DGLRDALDPRLARQL"
BASE COUNT          403 a         1077 c          962 g          493 t
ORIGIN      
        1 cacttcgacc gcgtcgaatg gctctacctg cccgattcga acagctcgac ggcggcgcta
       61 aagaacggcg aggtcgacat gatcgagcag gtgccgcccg actacatcac gcccctgcgc
      121 agcgacccga acatcacggt cggcagcggc ggctcttacc agggattcat cgtcttcaac
      181 cagctgttcc cgccgttcga caacccgaag gtgcgccagg cggtgctgca cgcggtgaac
      241 caggagaagt tcgtcgccgc gctcggctat ccgctcgaca tgcggatgac gtattgcgcg
      301 acctggttca tctgcggcgg cccgaaccag accgaggccg gcgccgagcc gttccgcaag
      361 gtcgacgtcg ccaaggcgaa gcagctcctg gcagaaggcg gctacaaggg cgagaaggtc
      421 gtcgtcctcg ttcccaccga catcacctac ctcaacgcgg gggcgctggt gacggtccag
      481 acgttgaagt cgatcggcat gaatgtcgac gcgcagacga gcgactgggc cacgatcacg
      541 gcgcgccggg ccaagaagag cgcgcccgac gccggcggct ggaacgttta cgtcaccgtc
      601 gccggcgagt tcgacgtcaa ttcgccgatg accaatgcct acctgagcgc ggcgtgcggc
      661 aactcgctgc ccggctggcc ttgcgacaag acgctcgacg agatacgcac ggcctgggtg
      721 cgcgagaccg tcccggccaa gcggaaggaa ctgctcgacc ggttccagga gcgcgccttc
      781 caggccgttc cctacgccta cttcggccag tactcgccgg cctatgcggc gcgcaagtcg
      841 atcaagcacc tcgacgaata ctggggcatc ccgacgctct gggcgctcga caagtagcgg
      901 gccccggctg aagccgagcg cgacgcggat cgaccggtca tgggaatgat tcttcgccgt
      961 gtcgccgcca cgttgccggt gatggcgatc gtcgcgctgc tcgtcttcct gctgatccac
     1021 ctcgcgccgg gcgatccggc cgccctgatc gccggcgacc tcgccagcac cgacgacatc
     1081 gccagactgc acaccagcct cggcctcgac caaccgctct ggcgccagtt cgccctctgg
     1141 gccgggcacc tggcgcgcgg cgacctgggc acctcgatct tcacgcaggt gccggtcgcc
     1201 gagctgctcg cgcagcggct cgagccgacg ctgtcgatcg ccgtcctgac gatcctgatc
     1261 tcgatcgtcg tcgcgattcc gctcggcacg ctggcggcct atcgggccgg ttcgtggatc
     1321 gaccgggcgg cgatgctgtt cgcggtgctg gcgttctcgg tgccggtctt cctcgtcggc
     1381 tacctgctga tctggggctt cgcgatccgc ctgcactggc tgccggtgca aggctacgcg
     1441 cgcctcggcg acggcgtcgg tgcgtggttc tcgcatctcg tgctgccgtg cgtgaacctg
     1501 gccctcgtct acatcgcgct cctgacccgc atgacccgcg ccaccgtgat cgaggttctc
     1561 caggaggact acatccgcac cgcgcgagcg aaggggctcg gcgtcctcgc cgtcctcgac
     1621 catgcgctgc gcaacgcggc gatcccgatc gcgacgaccg tcggcgtcgg catcgccctc
     1681 ctgatcggcg gcgtcgtcgt caccgagacc gtgttcgcga ttccgggcgt cggccggctc
     1741 gtcgtcgagt cggtgcaacg ccacgactat cccgtcatcc agagcgtcct gctgctgtcg
     1801 gccggtgcct atgtcctcgt caatctgttg atcgacctga gctacggact cttcgatccg
     1861 cgcatccgtg acgcctgagg ccgcggccac cggcgacgtg cggggcaggg cgtcgatcgc
     1921 agccgttgcg acgccgcctt cggcccgccg cggcccctgg agacgtccgc tcgcgtggct
     1981 gcttcgccac ccgacgctgg ccttcggcgc actgctgctc atcgccatgg cggcgatcgc
     2041 catcgccgct ccctggctgg cgacgcacga tcccgacgat atcgatccgc tggcgcgcct
     2101 gcagccgccg acagccgagc accggttcgg caccgacgcg ctcggccgcg acgtctacag
     2161 ccgcaccttg ctgggcgccc gcgtctcgct cgccgtcggc gtcgcggtcg cggccatctc
     2221 ggcgacgatc ggcatcgtcc tcggcctcgt cgccggcttc gtgcggccgc tcgagggcgt
     2281 gctgatgcgg accatggacg gcctgatggc cattcccgga atcctgctcg ccatcgccgc
     2341 gatggcggtc gttcgcgcca gtctcgccac cgtcatcgtc gcgatcacga tccccgaggt
     2401 gccgcgagtc gtgcgcctgg tgcgctcgct cgcgctgacg ctgcgcgagc agctctacgt
     2461 cgaggcggcg cgcgccgtcg gcacgcgctt gccggccatc ctctggcgcc acgtgctacc
     2521 caacatgatg gcgccgctga tcgtccaggc gactttcgtc gccgcatcgg cggtgctcat
     2581 cgaagcggcg ctgtccttcc tcggcgtcgg cgtgccgggg cagacgccga gctggggcaa
     2641 catgatggcc gagggacgca acttcgtcgc cgtcgccttc cacatcatcc tgtatcccgg
     2701 cctcttcctc gccacgacgg tgctggcgat caacctgctc ggcgacggcc tgcgcgatgc
     2761 gctcgacccg cgcctcgcac gccagctctg aagccggcat gagcgacgcg gccctgctcg
     2821 aagttgacgg actgcgcgtc gactttccgg gcctcgacgg cgccgcccgg gcggtcgacg
     2881 gcgtctcgtg gcggctcgag gcggggcgca cgctgggcct cgtcggcgag tcggg
//
LOCUS       DASRWS010000006         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4031530, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000006 DASRWS010000000
VERSION     DASRWS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4031530"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..474
                     /gene="pyrG"
                     /locus_tag="VFF72_00085"
                     /pseudo
     CDS             1..474
                     /gene="pyrG"
                     /locus_tag="VFF72_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346207.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
     gene            585..1442
                     /gene="kdsA"
                     /locus_tag="VFF72_00090"
     CDS             585..1442
                     /gene="kdsA"
                     /locus_tag="VFF72_00090"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZV91578.1"
                     /translation="MKLCGFDVGLDQPLFLIAGPCVVESQQLQVDVAGRLKETAAKLG
                     MPFIFKSSYDKANRSSGASFRGPGMDRGLEILAEVKRQLGVPLLTDVHREDEIAAAAA
                     VVDVLQTPAFLCRQTDFIRAVAQSGRPVNIKKGQFLAPGDMTNVLDKARAAAREKGLD
                     EDRFMACERGASFGYNNLVSDMRSLAIMRQTMAPVVFDATHSVQLPGGQGTSSGGQRE
                     FVPVLARAAVAVGVAGIFMETHPDPAKALSDGPNAVPLRHMPALLEQLVAIDRLVKSR
                     PFAENDFAA"
     gene            1510..1800
                     /locus_tag="VFF72_00095"
     CDS             1510..1800
                     /locus_tag="VFF72_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZV91579.1"
                     /translation="MSAYIVSNVTVTDPAQYELYKQLSSKAMAVHGPQVCVRGGAVEV
                     VEGDWRPDRVVILRFADVAAARAFYDSPEYVAARNARQGAATMRMVIVEGAD"
     gene            1883..3166
                     /gene="eno"
                     /locus_tag="VFF72_00100"
     CDS             1883..3166
                     /gene="eno"
                     /locus_tag="VFF72_00100"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZV91580.1"
                     /translation="MSAIVDIVGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTG
                     SREAIEMRDGDASRYGGKGVLKAVEHVNTEISEAVLGLDSSEQAFLDRTLVDLDGTDN
                     KSRLGANATLAVSMAVARAAAEESGLPLYRYFGGSGAMQMPVPMMNVVNGGAHANNNL
                     DLQEFMIIPVGAPSFREALRYGAEVFHALKKIIDGRGMSTAVGDEGGFAPSVASHEAA
                     IQMILEAIEKAGFAAGTQVALGLDCAASEFFRDGQYRLEGEGLTLAAGEWIDTLAGWV
                     DKYPILSIEDGMAEGDWDGWKQLNDRLGQKVQLVGDDIFVTNPRILKEGIEKKVANSV
                     LIKINQIGTLTETFAAIEMAKRAGWTSVISHRSGETEDSTIADIAVGTNAGQIKTGSL
                     SRSDRIAKYNQLLRIEEDLGEVATYPGRSAFHQAG"
     gene            3179..3457
                     /gene="ftsB"
                     /locus_tag="VFF72_00105"
     CDS             3179..3457
                     /gene="ftsB"
                     /locus_tag="VFF72_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HZV91581.1"
                     /translation="MRFVTLGLVALLALVHAELWFGKGGVPRMMELRSRLAAQQSAND
                     IARQRNEQLAAEVGDLKEGLEMVEEKARSELGMIKPDEILVQLAAHRH"
BASE COUNT          607 a         1246 c         1140 g          534 t
ORIGIN      
        1 ctcggcctcg cgggcgccaa cagcaccgag ttcgatccgc tcaccgagca cccggtgatc
       61 gccctcatcg acgagtagga agatcccgat ggctcggtgc aaaagcgcag cgcccagtcc
      121 gatctcggcg gcacgatgcg cctgggtgcg caaagctccg acgtcaaggc cggcacgttg
      181 gcccacgaga tctacggcaa cgtcgtcaac gaacggcacc gccatcgcta cgaggccaac
      241 gagcactacc tcgaccggtt gaccgaggcc gggctcgtca tctcggcgat cacgcaacgc
      301 gagaagctga ccgagatcgt cgagcttccg caggccacgc atccctggtt cgtcggcgtg
      361 cagttccatc ccgagttcaa gtcgacgccc tgggctggcc atccgctctt cacctcgttc
      421 atccacgcgg cgctcgagca ccaggccgcc cgcctgcgga aggtggcggc gtagatgacg
      481 aactctccac gctcgcgccg cttgctgagc cgcaaggaag cagcaatcgc cttggaaacg
      541 acccgacggg tatcgccgcg accgtccctg gcgcggactt gccgatgaag ctctgcggct
      601 tcgacgtcgg cctcgaccaa ccgctgttcc tgatcgccgg cccctgcgtc gtcgagtcgc
      661 agcagctgca ggtcgacgtc gccggccggc tgaaggagac ggcggcaaag ctcggtatgc
      721 ccttcatctt caagtcgagc tacgacaagg cgaatcgctc gagcggcgcg tcgtttcgcg
      781 gccccggcat ggatcggggc ctcgagatcc ttgccgaggt gaagcgccag ctcggcgtgc
      841 ccctcctcac cgacgtgcac cgcgaagacg agatcgccgc cgcggccgcc gtcgtcgacg
      901 tgctgcagac gccggcgttc ctttgccggc agaccgattt catccgcgcc gtcgcgcaaa
      961 gcggcaggcc cgtcaacatc aagaagggcc agttcctggc gccgggcgac atgacgaacg
     1021 tgctcgacaa ggcgcgtgcg gcggcgcgcg aaaagggcct cgacgaggac cggttcatgg
     1081 cctgcgagcg cggcgcgagc ttcggctaca acaacctcgt ttccgacatg cgctcgctgg
     1141 cgatcatgcg ccagacgatg gcgcccgtcg tcttcgacgc gacccattcg gtgcagttgc
     1201 ccggcggcca gggcacgtcg tcgggcggcc agcgcgagtt cgttccggtg ctcgcccgcg
     1261 cggcggtcgc cgtcggcgtc gccggcatct tcatggagac gcaccccgat cccgccaagg
     1321 cgctctccga cggcccgaac gcggtgccgc tcaggcacat gccggccttg ctcgagcaac
     1381 ttgtcgccat cgacaggctc gtcaagtcgc ggccgttcgc cgagaacgac ttcgccgcct
     1441 gatgacctgc actgcacggc cgccgatcga cccagccgtt cttgcccaca ccccttacct
     1501 tccgatgcca tgagcgccta catcgtctcc aacgtcaccg tcaccgaccc cgcgcagtac
     1561 gagctgtaca agcagctctc gtcgaaggcg atggccgtgc acggtccgca ggtgtgcgtc
     1621 cgcggcggcg cggtcgaggt cgtcgagggc gactggcggc ccgaccgtgt cgtcatcctg
     1681 cgcttcgccg acgtcgccgc cgcacgcgcc ttctacgact cgccggaata cgtcgccgca
     1741 cgcaacgccc gccagggcgc ggcgaccatg cgcatggtca tcgtcgaggg cgccgactga
     1801 tggccgcgac ggccgccgaa aatttcttcc gcccggtcgc aggcgcgacg ggcccttcga
     1861 attcccagcc agaggtccat tcatgagcgc catcgtcgac atcgtcggca gagaaatcct
     1921 cgacagccgc ggcaatccga ccgtcgagtg cgacgtcctt ctcgagtcgg gaaccatggg
     1981 ccgcgccgcc gtcccctcgg gcgcctcgac cggctcgcgc gaggcgatcg agatgcgcga
     2041 cggcgatgca tcgcgctacg gcggcaaggg cgtgctcaag gccgtcgagc acgtcaacac
     2101 cgagatcagc gaagccgtcc tcgggctcga ctcgagcgag caggcgtttc tcgatcgcac
     2161 gctcgtcgac ctcgacggca ccgacaacaa gagccggctc ggtgccaacg cgacgctcgc
     2221 cgtcagcatg gcggtggcgc gggcggcggc cgaggagtcg ggcctgccgc tctaccggta
     2281 tttcggcggc tcgggggcga tgcagatgcc ggtgccgatg atgaacgtcg tcaacggcgg
     2341 cgcccacgcc aacaacaatc tcgacctgca ggagttcatg atcattccgg tcggcgcgcc
     2401 ttcttttcgc gaggcgttgc gctacggtgc cgaggtgttc catgccctga agaagatcat
     2461 cgacggccgc ggcatgagca ccgcggtcgg cgacgagggc ggtttcgcgc cgagcgtggc
     2521 cagccacgaa gcggcaatcc agatgatcct cgaggcgatc gagaaggccg gcttcgcggc
     2581 cggcacgcag gttgcgctcg gcctcgactg cgccgccagc gagttcttcc gcgacgggca
     2641 ataccgtctc gagggcgaag gcctgacact ggccgccggc gaatggatcg acacgctcgc
     2701 aggctgggtc gacaaatacc ccatcctgtc gatcgaggac ggcatggccg aaggcgactg
     2761 ggacggctgg aagcagctca acgaccggct cggccagaag gtccagctcg tcggcgacga
     2821 catcttcgtc accaacccgc ggatcctgaa agaaggcatc gagaagaagg tcgccaactc
     2881 ggtcctgatc aagatcaacc agatcggcac gctgaccgag accttcgccg ccatcgagat
     2941 ggccaagcgc gccggctgga cttcggtcat cagccatcga tcgggcgaga ccgaagactc
     3001 gaccatcgcc gacatcgccg tcggcaccaa cgccggccag atcaagaccg gctcgctcag
     3061 tcgctccgac cgcatcgcca agtacaacca gctgttgcgg atcgaggaag acctgggcga
     3121 ggtggccacc tatcccggcc gcagcgcctt ccaccaggcc ggctgaggca ggttcacgat
     3181 gcgattcgtc acgctgggtc tggtcgccct gctcgccctc gtccacgccg agctgtggtt
     3241 cggcaagggc ggcgtgccgc ggatgatgga gcttcgctcg cggctggccg cgcagcagtc
     3301 ggccaacgac atcgccaggc agcgcaacga gcagctcgcg gccgaggtcg gcgacctgaa
     3361 ggaagggctg gaaatggtcg aggaaaaggc gcgctccgag ctcggcatga tcaagcccga
     3421 cgagatcctc gtccagctcg ccgcccaccg gcattgaact cgctgatcgc ggcgcttcag
     3481 ccgctcttcg ccggcgcctt ggtcgtcgcc ggcagcgtcg tcacctg
//
LOCUS       DASRWS010000007         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1442389, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000007 DASRWS010000000
VERSION     DASRWS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442389"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3254)
                     /locus_tag="VFF72_00110"
     CDS             complement(<1..3254)
                     /locus_tag="VFF72_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HZV91582.1"
                     /translation="MNAPLPMAEMSDLASTAETAGGLPRRREIPYNYTSFSDREIVIR
                     LLGEHAWSLLDLLRAERRTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNPRRRNLLIE
                     ALQHRLGEIERRRTPADDAARDAVVGELLGVARAAVDAFSAHFREVWDLRKRAVRRLA
                     RATAKGNVKFDALSRVSHVTDATDWRIEYPFVVLTPDSEAEMAALVAGCIELGLTIIP
                     RGGGTGYTGGAIPLTWKSAVVNTEKIDALNEVEMVHLPGVDGEVATVWSEAGVVTQRV
                     ADAAERSGYVFAVDPTSAEASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVTPDATW
                     LEVTRLDHNLGKIHDAPTASFELRYLDASGRKEVRRERLDIPGSAFRKEGLGKDVTDK
                     FLAGLPGIQKEGCDGLVTSARWIVHRMPEHVRTVCLEFYGNAKDAVPSIVEIRDYMFG
                     LARTPHGARVLLAGLEHLDDRYLKAVGYAKKSKRGGDALPKMVLFGDIAGDDADAVAR
                     ATSEVIRIANSRSGEGFVAISAEARKKFWLDRKRTAAISKHTNAFKINEDVVIPLDRM
                     GEYTEGIERINIELSLANKIELVERLETFFRAGNLPLGKGDDAGEIASAEILEDRVQQ
                     ALALLRETAGLWRGWLLRLDEPADEDPSRTVFALLQDHTLRASWKTQVRAPLQSIFGG
                     AALKPIVGECQRIHDEVLRGRVWAALHMHAGDGNVHTNIPVNSDNYEMLQTAHAAVAR
                     IMALARSLGGVISGEHGIGITKLEFVSDEELAAFADYKARVDPEGRFNQGKLLRGVVF
                     GPAGAPLRADLTNAYTPSFGLMGHESLIMQQSDIGEIANSIKDCLRCGKCKPVCATHV
                     PRANLLYSPRNKILATSLLVEAFLYEEQTRRGISIRHWEEFEDVADHCTVCHKCLSPC
                     PVDIDFGDVSMNMRNLLRKMGRKSFRPAGAAAMFFLGATRPETIKVARAAMVGVGFRL
                     QRSANDLLRGLANQQTARPPSTVGKPPLKEQVVHFINKKMPGGLPNRTARALLDIEDK
                     NYVPVIRNPKTTNAETEAVFYFPGCGSERLFSQVGLATQAMLYHVGAQCVLPPG"
BASE COUNT          504 a         1143 c         1129 g          580 t
ORIGIN      
        1 taccccggcg ggagcacgca ttgcgcgccc acgtgataga gcatcgcctg cgtggccagg
       61 ccgacctgcg agaacaggcg ctccgatccg caaccgggga agtagaagac agcctcggtc
      121 tcggcattcg tcgtcttcgg attgcggatg acggggacgt agttcttgtc ctcgatgtcg
      181 agaagcgcgc gcgccgtccg gttcggcagg ccgccgggca tcttcttgtt gatgaaatgg
      241 acgacctgtt ccttcagcgg cggcttgccc accgtcgacg gcggcctggc cgtctgctgg
      301 ttggccaagc cgcgcagcag gtcgttggcg ctgcgctgca gacgaaagcc cacgcccacc
      361 atcgccgcgc gggcgacctt gatcgtctcg ggccgcgtcg cgcccagaaa gaacatcgcc
      421 gccgcgccgg ccggtcgaaa gctctttcgc cccatcttgc gcagcaggtt tctcatgttc
      481 atcgagacgt cgccgaagtc gatgtcgacc gggcacggcg acagacactt gtggcagacc
      541 gtgcagtgat cggcgacgtc ctcgaactcc tcccagtggc ggatcgagat gccgcgccga
      601 gtctgctcct cgtagagaaa ggcctcgacg agcaacgagg tcgcgaggat cttgttgcgc
      661 gggctgtaga gcaggttggc gcgcggcaca tgcgtcgcgc agaccggctt gcacttgccg
      721 cagcgcaggc agtccttgat cgaattcgcg atctcgccga tatcgctctg ctgcatgatg
      781 agcgactcgt ggcccatcag gccgaagctc ggcgtgtagg cgttcgtcag gtcggcccgc
      841 agcggcgcgc cggccgggcc gaagacgacg ccgcgaagca gcttgccctg gttgaagcgg
      901 ccctcgggat cgacccgggc cttgtagtcg gcgaaagccg ccagctcctc gtcgctcacg
      961 aactcgagct tggtgatgcc gatgccgtgc tcgccggaga tcacgccgcc caggctgcgc
     1021 gccagcgcca tgatgcgggc gacggcggcg tgcgccgtct gcagcatctc gtagttgtcg
     1081 ctgttgacgg gaatgttggt gtgcacgttg ccgtcgccgg catgcatgtg cagcgccgcc
     1141 cacacccggc cccgcagcac ctcgtcgtgg atgcgctggc attcgccgac gatcggcttc
     1201 agcgcggcgc cgccgaagat cgactgcagc ggtgcgcgga cctgcgtctt ccacgaggcg
     1261 cgcagcgtgt ggtcctgcag cagcgcgaag acggtgcgcg atggatcttc gtcggccggc
     1321 tcatcgagcc gcaatagcca gccgcgccac aggccggcgg tctcccgcag cagtgccagg
     1381 gcctgctgga cgcggtcttc caagatctcg gccgaggcga tctcgccggc gtcgtcgccc
     1441 ttgcccagcg gcaggttccc ggcccgaaag aaggtctcga ggcgctcgac gagctcgatc
     1501 ttgttggcca ggctgagctc gatgttgatg cgctcgatgc cctcggtgta ctcgcccatc
     1561 cggtcgagcg ggatgacgac gtcctcgttg atcttgaagg cgttggtgtg cttgctgatc
     1621 gccgcggtgc gcttgcgatc gagccagaac ttcttgcgtg cctcggcgct gatggccacg
     1681 aaaccctcgc cggaccggct gttggcgatg cggatcacct cgctggtcgc ccgcgcgacg
     1741 gcgtcggcgt cgtcgccggc gatgtcgccg aaaagaacca tcttcggcaa cgcgtcgcca
     1801 ccgcgcttgc tcttcttcgc gtagccgacg gccttcaggt agcggtcgtc gagatgctcg
     1861 aggccggcca gcagcacgcg cgcgccgtgc ggtgtgcgcg ccagaccgaa catgtagtcg
     1921 cgtatctcga cgatcgacgg cacggcatcc ttggcgttgc cgtagaactc gaggcacacc
     1981 gtgcgcacgt gctcgggcat gcggtggacg atccagcgcg ccgacgtgac gaggccgtcg
     2041 cagccctcct tctggatgcc gggcagtccg gcgaggaact tgtcggtgac gtccttgccg
     2101 agaccttcct tgcgaaaggc cgaccccggg atgtcgagcc tctcgcggcg gacttccttc
     2161 ctgccgctgg catcgagata cctgagctcg aagctcgcgg tgggcgcatc gtggatcttg
     2221 cccaggttgt ggtcgagccg cgtcacctcg agccaggtcg cgtcgggcgt caccatccgc
     2281 cacgacgcca ggttgtcgag ggccgtgccc cagagcaccg ccttcttgcc gccggcattc
     2341 atcgccacgt tgccgccgat gcaggacgcc tcggccgacg tcggatcgac ggcgaagacg
     2401 tatcccgagc gctcggcggc atcggcgacg cgctgggtga cgacgccggc ttcgctccac
     2461 accgtcgcca cctcgccgtc gacaccgggc agatgcacca tttccacttc gttcagcgcg
     2521 tcgatctttt cggtgttgac gaccgcgctt ttccaggtga gcgggatcgc tccaccggtg
     2581 tagccggtgc cgccgccgcg cggaatgatc gtcaggccca gctcgatgca gccggcgacg
     2641 agcgccgcca tctccgcctc gctgtcgggc gtcagcacga cgaagggata ttcgatccgc
     2701 cagtcggtcg cgtcggtcac gtgggacacg cgagacaacg cgtcgaactt gacgttgccc
     2761 ttggcggtcg cccgcgccaa cctgcggacg gcgcgcttgc gcagatccca gacctcgcga
     2821 aagtgcgccg agaacgcgtc gacggcggca cgcgccacac cgagcagctc gcccacgacc
     2881 gcgtcgcgcg ccgcgtcgtc ggccggcgtg cgccgccgtt cgatctctcc cagccggtgc
     2941 tgcagcgcct cgatcagcag attgcgacgg cgcgggttgt cgagcaggtc atcctgcaga
     3001 taggggttgc gctgcaccac ccagatgtcg cccagcacct cgtagagcat ccgcgccgat
     3061 cggccggtgc ggcgctctgc gcgaagcaaa tcgagcaggc tccacgcgtg ttcaccgagc
     3121 aggcgaatga cgatctcgcg atcggagaac gacgtgtagt tgtagggaat ctcgcgccgg
     3181 cgtggcagcc cccctgccgt ctcggccgtg gaggcaaggt cgctcatctc ggccatcggc
     3241 agcggggcgt tcattcggcg aggcgcccgc ggcgccttct tatcggggtc cgagtcatcg
     3301 taccgcagcg cggcaagcca actgtgacgc ggcggttgcg gcggataatg cccgtt
//
LOCUS       DASRWS010000008         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2018218, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000008 DASRWS010000000
VERSION     DASRWS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018218"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFF72_00115"
     CDS             complement(<1..380)
                     /locus_tag="VFF72_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZV91583.1"
                     /translation="MHDIIQQLEAKRASALRGGGEKRIAAQHAKGKLTARERLELLLD
                     EGSFEEWDMFVEHRSSDFGMEANRPPGDGVVTGHGTINGRLVFVFSQDFTVFGGALSE
                     AHAEKICKIMDQAMKVGAPVIGLN"
     gene            complement(385..1158)
                     /locus_tag="VFF72_00120"
     CDS             complement(385..1158)
                     /locus_tag="VFF72_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91584.1"
                     /translation="MTAAELDGLRAVVGSQPHRIRVVETAAGRVVVKQQRPSRSPWRG
                     RALNLLARALGLRLLQAVPAHGGARGQAIEVDRLRRLAQAGVRVPPVLHVEGDFFVVA
                     YLDGRPLVEIIERGGVAGVAAWQRGLAALVDLHRRGGYLSHAFARNFLDSPAGLAMID
                     FEDDPLEAMTLAEAQARDWLAYLHSTAWLLPRDIARDALARHLASERADVRALVEDAG
                     RRLAMLRHLPRTRRVWGREVVGAQAAASLLSPAPAPAGQ"
     gene            complement(1155..2138)
                     /gene="meaB"
                     /locus_tag="VFF72_00125"
     CDS             complement(1155..2138)
                     /gene="meaB"
                     /locus_tag="VFF72_00125"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZV91585.1"
                     /translation="MLPPVDQSLLDDVTADGERQRRAVAKTITLLESTRADHRRRAEA
                     LLAALPASAGPTLRLGISGVPGVGKSTFIEALGLSLIGAGHRVAVLAVDPSSSLSGGS
                     ILGDKTRMERLSVHARSFVRPSPNAGTLGGVAAATRDAIRVVEAAGYDVVVVETVGIG
                     QSEAAVAGMTDCFVLLQLPNAGDDLQAIKKGVLELADLVVVNKADLDPRAAARAVGQI
                     ESAMRLVAGRTAPRVIAASALDEARVDEVRQAIASFVDAQRAGDALAARRRGQARASL
                     WERIDTGLRDGFRADPAVRALLPQALDDIDAGTIPVSSVARRLLAAFTRSA"
BASE COUNT          337 a          796 c          808 g          301 t
ORIGIN      
        1 tcgttgaggc cgatcaccgg cgcgccgacc ttcatcgcct ggtccatgat cttgcagatc
       61 ttctcggcgt gcgcctcgga gagcgcgccg ccaaagacgg tgaagtcctg gctgaagacg
      121 aagacgaggc ggccgttgat cgtgccgtgc ccggtgacga cgccgtcgcc gggcggccgg
      181 ttcgcttcca tgccgaaatc ggagcttcga tgctcgacga acatgtccca ctcctcgaag
      241 ctgccctcgt cgagaaggag ttcgagtcgt tcgcgcgcag tcagcttgcc tttcgcatgc
      301 tgggcggcga tgcgcttttc gccgccgcca cggagagcac tcgcgcgctt ggcttcgagc
      361 tgctggatga tgtcgtgcat ggcgctattg tccggccggc gccggggcag gagaaagaag
      421 gcttgccgcc gcctgcgcac cgacgacttc gcggccccag acgcgccgcg tgcgcggcag
      481 gtgacgaagc atggccaggc gccgcccggc gtcctcgacg agcgcgcgaa catcggcccg
      541 ctcgctggca aggtgccgcg ccagcgcgtc ccgggcgatg tcgcgtggca gcagccacgc
      601 cgtcgagtgc aggtaggcga gccagtcgcg cgcctgcgcc tcggccagcg tcatcgcctc
      661 gagcggatcg tcctcgaagt cgatcatcgc cagaccggcc ggcgagtcga ggaagttgcg
      721 cgcgaaggca tggctcaggt agccgccgcg ccggtgcagg tcgacgagag cagccagacc
      781 gcgctgccat gcggcgacgc cggcgacacc accgcgctcg atgatctcga cgagcggccg
      841 gccatcgaga tacgcgacga cgaagaagtc gccctcgaca tgaagaaccg gcggcacacg
      901 tacgccggcc tgtgccagcc gacggagtcg atcgacctcg atggcctggc cgcgcgcgcc
      961 gccatgcgcc ggcacggcct ggagcaggcg caggccgagc gcgcgcgcca gcagattcag
     1021 ggcgcggccg cgccatgggc tgcgtgaggg ccgctgctgc ttgacgacga cgcggcccgc
     1081 tgcggtctcg accacccgga tgcgatgcgg ctggctgccg acgacagcgc gtagcccgtc
     1141 gagctcggcc gcggtcacgc gcttctggtg aacgcggcga gcaggcgccg cgccaccgac
     1201 gacacgggga tcgtccccgc gtcgatgtcg tccagtgcct gcggcaagag cgcccgcacc
     1261 gcggggtcgg cgcgaaagcc gtcgcgcagc ccggtgtcga tgcgctccca cagcgacgcc
     1321 cgcgcctggc ctcggcggcg agcggcgaga gcatcgcccg cccgctgtgc gtcgacgaac
     1381 gacgcgatcg cctggcgcac ctcgtcgacc cgcgcctcgt cgagcgcgct cgcggcgatg
     1441 acgcgcggcg cagtgcggcc ggcgacgagg cgcatggccg actcgatctg gccgacggcg
     1501 cgcgccgccg cgcggggatc gagatcggcc ttgttgacga caacgaggtc ggcgagttcg
     1561 aggacgcctt tcttgatcgc ctgcaggtcg tcgccggcat tgggaagctg caacaggaca
     1621 aagcagtcgg tcatgccggc gacggccgct tcgctctggc cgatgccgac cgtctcgacg
     1681 acgaccacat cgtagccggc agcctcgacg acacgaatcg cgtctcgcgt tgccgcggcg
     1741 acaccgccga gcgtgcctgc gttcgggctc ggccgcacga aggaccgggc gtggaccgac
     1801 aggcgctcca tgcgcgtctt gtcgccgagg atcgagccgc ccgacaggct cgagctcgga
     1861 tcgaccgcca gcaccgcgac gcgatgaccg gcgccgatca gcgacaggcc gagcgcctcg
     1921 atgaaggtcg acttgccgac ccccggcacg ccggaaatgc ccaggcgcaa cgtcggtccg
     1981 gccgaggccg gcaatgcggc cagcaaggcc tcggcgcggc gacgatgatc ggcgcgggtc
     2041 gattccagca gcgtgatcgt cttggccacg gcccggcgct ggcgctcgcc gtcggccgtc
     2101 acgtcgtcga gcagcgactg atcgacgggt ggcagcatcg caaggcggat cggcgtcgcg
     2161 gtccgtcagg ccgcggcgac ggcgaggcgg atctgctcga ggacgtcctt ggcgctcgcc
     2221 ggaatcggcg tccccggacc gt
//
LOCUS       DASRWS010000009         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1731026, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000009 DASRWS010000000
VERSION     DASRWS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731026"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VFF72_00130"
     CDS             <1..1788
                     /locus_tag="VFF72_00130"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HZV91586.1"
                     /translation="GGSGGNSNGINIGNQYPASGAAPVQFVGLGAASVNVTGIGGSGV
                     NGGNGLQWVSGGSTGSLVIQTAGTGNVTVTGVAGSGGSGIATNNASSVGSTGSGSVTL
                     VADSLVLGAANTVSSATDLTIRPVTSGTTIGIGSGAAGSLGLSDSELAQLGWGRLLTI
                     GGASAGTTTIDTAFAFANPLAVVSGAGADIDLASPLRSTSGATTPTLTLSAGRNFVNG
                     AGAAALDPGAGVWRVYSSDPAFDTRDGLAPDFKQYAALYDTTPVQGSGNGALYRIAPS
                     LTPSLTGTIGKIYDGTDTAALTPANYAVTGAIDGDSVVVGNGSAAYDTRNAGTGKLVG
                     ATGIGIASATQGAIPVYGYALSDAAAAAPIGTIVPAALSLGATADSKVYDGSTRSSAM
                     PVVSGLVAGDSVTSLTQTFDGSAAGARTLGISTYTINDGNGGANYQVTALTAAGRIAP
                     APLVVTADDKGRTTWQPNPALTASYDGLVAGQTPADLTGTLVLTTAATPSSPAGSYAI
                     VAGGQSSANYSIRYVDGTLRIAPALGNGPTPPDSSQSQRDARLLAASIFFAPGCRPPA
                     EQAHTCLGETACRPKLPPCDSLTVAPSGR"
     gene            1875..>2748
                     /locus_tag="VFF72_00135"
     CDS             1875..>2748
                     /locus_tag="VFF72_00135"
                     /inference="COORDINATES: protein motif:HMM:NF020068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZV91587.1"
                     /translation="MLWLAAALPARAADPVTFSIRAFAVEGNTVLAASLVDATVAPYA
                     GPARTFADVHSAVAALQALYAQRGFGTVTVVLPEQQVSAGTVRLRVVEARLRRIGVKG
                     PAGFDATRIGRILPSLRVAATPNTADLAREIRLANENPARRLSIELTSVAADEIDAVV
                     TVVEDKPWKAGAVFDNTGTPSTGPTRIGAFVQYADVAGQDQVATLQYVTSPDRPSDVT
                     IAAVNYRVPLPRLGDSVDLYGVYADVDSGVVGDLFDVRGSGTVTGLRYNQNLRPTASL
                     QQRWTYGLEQRRTDN"
BASE COUNT          422 a         1051 c          930 g          345 t
ORIGIN      
        1 ggcggctccg gcggcaacag caacggcatc aacatcggca accagtaccc cgccagcgga
       61 gccgcgccgg tgcagttcgt cggcctcggc gcggcgtcgg tgaacgtgac gggcatcggc
      121 ggcagcggcg tcaacggcgg caacggactg caatgggtca gcggcggcag caccggctcc
      181 ctcgtcatcc agactgccgg cacgggcaac gtcaccgtca ccggcgtcgc cggttccggc
      241 ggcagcggca tcgccaccaa caacgccagc agcgtcggga gcaccggcag cggcagcgtc
      301 acgctcgtcg ccgacagcct ggtgctcggc gcggccaaca cggtcagcag cgcgaccgac
      361 ctgacgatcc ggcccgtcac cagcggcacc acgataggca tcggcagcgg cgcggcgggc
      421 tcgctcggcc tgagcgacag cgagctggcg cagctcggct ggggcaggct gctgacgatc
      481 ggtggcgcga gcgcaggcac gacgacgatc gacacggcgt tcgctttcgc caatccgctc
      541 gcggtcgtct cgggcgccgg cgccgacatc gatctcgcgt cgcccctgag gagcacgagc
      601 ggcgccacca cgccgaccct gacgctcagc gccggccgca acttcgtcaa cggcgcaggc
      661 gcagcggcgc tcgacccggg cgccggcgtc tggcgggtct attcgagcga tcccgccttc
      721 gacacgcgcg acggcctagc gcccgacttc aagcagtacg ccgccctcta cgacacgacg
      781 cccgtgcaag gcagcggcaa cggcgccctc taccgcatcg cgccgagcct gacgccgtcg
      841 ctgaccggca cgatcggcaa gatctacgac ggcaccgaca ccgccgccct cacgccggcc
      901 aactacgcgg tcaccggagc gatcgacggc gacagcgtcg tcgtcggcaa tggcagcgcc
      961 gcctacgaca cgcgcaacgc cggcaccggc aagctcgtcg gcgccaccgg catcggcatc
     1021 gcgagcgcga cccagggggc gataccggtc tacggatatg cgctctccga cgccgcggcc
     1081 gccgctccga tcggcacgat cgtgccggcc gcgctgagcc tgggcgcgac ggccgacagc
     1141 aaggtctacg acggcagcac gcgctcgtcg gcgatgcccg tcgtctccgg gctcgtcgcc
     1201 ggcgacagcg tgacgtcgct gacgcagacc ttcgacggca gcgctgcggg cgcgcgaacg
     1261 ctcggcatct cgacgtacac gatcaacgac ggcaacggcg gggcgaacta ccaggtcacg
     1321 gcactcaccg cggcgggaag gattgcgccg gcgccgctcg tcgtcaccgc cgacgacaag
     1381 ggtcgcacga cctggcagcc caacccggcg ctgaccgcaa gctacgacgg cctcgtcgcc
     1441 gggcagacgc cggccgatct gacgggcacg ctcgtgctga ccaccgcggc caccccgagc
     1501 tcgccggccg gaagttacgc gatcgtcgcg ggcggccaga gctctgcgaa ctactcgatc
     1561 cgctacgtcg atggcacgct ccggatcgcg cccgcgctcg gcaacgggcc gacgccgccg
     1621 gactcgagcc agtcgcagcg cgacgcgcgg ctgctggcgg cctcgatctt cttcgccccg
     1681 ggatgccgac cgcccgcgga gcaggcacat acctgcctcg gcgaaacggc atgccgtccg
     1741 aagctgccgc cgtgcgattc gctcaccgtc gcgccgtcgg gccgatgacc ggccgcgctc
     1801 gtaccgcgcg ggcgaccgga cccggcctcc ctgggccgct gcgccgatgg gcgaaggctg
     1861 gcgcgttcgc ggtcttgctg tggctggcgg ccgcgctgcc ggcccgggcc gcagatccag
     1921 tgacgttctc gatccgcgcc ttcgccgtcg agggcaacac ggtgcttgcc gcttcgctcg
     1981 tcgacgccac cgtcgcgccc tatgccggcc ccgcccgcac cttcgccgat gtgcattccg
     2041 cagtggccgc attgcaggcg ctctacgcgc aacgcggctt cggcacggtg accgtcgtcc
     2101 tgcccgagca gcaggtcagc gccggaacgg tgcgcctgcg cgtcgtcgaa gcacggctgc
     2161 gtcgcatcgg cgtcaaggga cccgccggct tcgatgccac gcgaatcggc aggatcctgc
     2221 cttcgctgcg cgtggcggcg acgccgaata ccgccgacct ggcgcgcgag atccgtctcg
     2281 ccaatgaaaa cccggcgcgg cgactgagca tcgaattgac gagcgttgcg gccgacgaga
     2341 tcgatgccgt cgtgacggtg gtcgaggaca agccatggaa ggcgggcgcc gtcttcgaca
     2401 acaccggaac cccgtcgacc gggcccaccc ggatcggcgc cttcgtgcaa tacgccgacg
     2461 tcgccggcca ggaccaggtc gcgacgcttc agtacgtcac ctccccggac cgcccgtccg
     2521 acgtgacgat cgcggcggtg aactaccgcg tcccgctgcc gcgcctgggc gactcggtcg
     2581 acctctacgg cgtctacgcc gacgtcgact cgggcgtcgt cggcgatctc ttcgacgttc
     2641 gcggcagcgg taccgtgacg ggcctgcgct acaaccagaa tcttcgcccg accgcgagcc
     2701 tgcagcagcg ctggacctac ggcctcgagc agcggcgcac cgacaacc
//
LOCUS       DASRWS010000010         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3457545, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000010 DASRWS010000000
VERSION     DASRWS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457545"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2082)
                     /locus_tag="VFF72_00140"
     CDS             complement(<1..2082)
                     /locus_tag="VFF72_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZV91588.1"
                     /translation="MKSESARPPEAAAAIVRLLDAPAAGAAAEGVDGGASDLVGRART
                     FAAPLVAGRTLPTGEAVLAHADATATILEGIGAAPELRAAAYLVHAADQLGQPDEVLA
                     PTFGESLTSLVAHARRLGDLQRTAQAARLGADERVRQTERARKMLLAFSRDLRVVLLR
                     LASRLQTLRHFAASKTPCPDALALEAQQVFAPLANRLGIWQIKWELEDLAFRFLDAEN
                     YRAIASLLDERRVDREARVARLRDALLADLAAHGIGAEVHGRPKHLYSIWKKMQRKSL
                     GFERVMDTIALRVIVADVAACYGVLGRVHERYPAIAGELDDYIARPKANGYRSLHTVV
                     EVEGRPVEIQIRTKEMHEHAEFGVSAHWAYKEADAPRAAPNAAGRFEADVAAARLVVL
                     RQLLAWERDLVAGDGAGDASEGAGSSPAIFEDRIYVFTPQAALVELAAGSTPIDFAYA
                     MHTDLGHRCRGAKVDGAMVSLNTPLRSGQTVEITAARSGGPSLDWLNPELGYLRSARA
                     RAKVRSWFNAIAQQATVARGREAVEKVLQREGRTSLKLEDLAAQLGFGSAEALFEVVG
                     KDEFSLRSIEALLRPTPAPKVGDEADIAVKPSRAVPGSNGGVLVVGVESLLTTLARCC
                     RPAPPDAIAGYVTRGKGVAVHRAGCSNLRELASRAPGRIIPVAWGVPGTAAGSTLYPV
                     DVSVEANDRQGL"
     gene            complement(2079..2975)
                     /locus_tag="VFF72_00145"
     CDS             complement(2079..2975)
                     /locus_tag="VFF72_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV91589.1"
                     /translation="MTTNENAAPAEAGPRLKYVQCLGAAGLHRMAYWEWGDPRCARVL
                     VCVHGLGRQGRDFDTLARAMRDSYRVVCPDVVGRGESDRLADPMGYQVPAYVADMVTL
                     IARLDVAEVAWLGTSMGGLIGMALAGLPNTPIERLVLNDVGPAIDAAGIARIAGYVGA
                     PLSWASEDEAADYLSTISRGFGPHSREDWMALTRPMLRRDGGRFRLHYDRAIAVALRA
                     VTPAAAAAGEAALWAAFDAIRCPTLVLRGRDSDVLSAATAKAMAERGPKARVREIEGV
                     GHAPTLVAADQVELVREFLLEP"
     gene            complement(2972..3703)
                     /locus_tag="VFF72_00150"
     CDS             complement(2972..3703)
                     /locus_tag="VFF72_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913875.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZV91590.1"
                     /translation="MTFDLHRLSFAAVATLCLAFPLSGVAQGAPPLQGVLGLSSSASV
                     DVAMDVIVVTFAATKEGADAGAVQSQLKQALDTALAEARKVARAGEVDVQTGNFSIWP
                     RYATTASSSRTTISGWQGSAQLIVEGRDMATIGALTGRISTMSISNVGFRLSHEAGRK
                     VEADVAAQAIARFRAKAEDYVKQFGYSGYAIREVNVAGNDAGFRPQPMLMRAQVASVA
                     RSEPLPVEPGKATITATVNGTVQMK"
     gene            3813..>4030
                     /locus_tag="VFF72_00155"
     CDS             3813..>4030
                     /locus_tag="VFF72_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV91591.1"
                     /translation="MNTTANARPDRIVVVDDDARIRDLLRRYLTQEGFEVLLAEDAKA
                     LNRLLTRETVHLIVLDLMLPGEDGLSICR"
BASE COUNT          594 a         1445 c         1421 g          570 t
ORIGIN      
        1 caacccctgc cggtcgttcg cctcgacgct gacgtcgacc ggatagagcg tgctgcctgc
       61 cgccgtgccg ggaacgcccc acgccaccgg aatgatccgc cccggagccc ggctcgcaag
      121 ctcgcgcagg ttgctgcagc cggcgcgatg gaccgcgacg cccttgccgc gcgtgacgta
      181 gccggcgatg gcatcgggcg gcgccgggcg gcagcatcgc gccagcgtcg tcagcagcga
      241 ctcgacgccg acgacgagca caccgccatt gctccccggc acggcgcgcg aaggcttgac
      301 ggcgatatcg gcctcgtccc cgaccttggg cgcgggcgtt ggacgcagca gcgcctcgat
      361 gctgcgcaac gagaactcgt ccttgccgac gacctcgaac agcgcctcgg cgctcccgaa
      421 gccgagctgc gcggcgaggt cttcgagctt gagcgaggtg cggccctcgc gctgcagcac
      481 cttttccacc gcctcgcgtc cgcgcgccac ggtcgcctgc tgggcgatcg cgttgaacca
      541 ggaacggacc ttggcgcgtg cccgcgcgct gcgcaggtag ccgagctcgg gattgagcca
      601 gtcgagcgac gggccgccgc tgcgcgccgc cgtgatctcg acggtctgac cgcttcgcaa
      661 cggcgtgttc agcgagacca tcgcgccgtc gaccttggcg ccgcggcaac ggtggccgag
      721 atcggtatgc atcgcgtagg cgaaatcgat cggcgtcgag ccggcggcga gctcgacgag
      781 tgcggcctgc ggcgtgaaga cgtagatgcg gtcctcgaag atcgccggcg acgacccggc
      841 gccctcgctg gcgtcgccgg caccgtcgcc ggcgacgaga tcgcgctccc aggcgagcag
      901 ctggcgcagc accaccaggc gcgccgcggc gacgtcggcc tcgaaccggc cggcagcgtt
      961 cggcgcggcg cgaggcgcgt ctgcctcctt gtaggcccag tgcgccgaga cgccgaactc
     1021 ggcatgctcg tgcatctcct tcgtgcggat ctggatctcg accggccggc cctcgacctc
     1081 gaccaccgtg tgcagcgagc ggtagccgtt ggccttcggc cgggcgatgt agtcgtcgag
     1141 ctcgccggcg atcgccggat agcgctcgtg gacgcggccg agcacgccgt agcaggcggc
     1201 gacgtcggcg acgatgacgc gcagcgcgat cgtgtccatc acgcgctcga agccgagcga
     1261 cttgcgctgc atcttcttcc agatgctgta gagatgcttg ggccggccgt gcacttcggc
     1321 gccgatgccg tgcgccgcga gatcggcgag gagcgcgtcg cgaagccgcg ccaccctcgc
     1381 ctcgcgatcg acgcggcgct cgtcgagcag gctggcgatg gcccggtagt tctccgcgtc
     1441 gaggaagcga aacgccaggt cctcgagctc ccacttgatc tgccagatgc cgagccggtt
     1501 ggcgagcggc gcgaacacct gctgcgcctc gagggccagc gcatcgggac agggtgtctt
     1561 gctcgccgcg aagtgacgca gcgtctgcag gcgggacgcg agcctcagca gcacgacgcg
     1621 caggtcgcgc gagaacgcga gcagcatctt gcgcgcgcgc tcggtctgcc ggacgcgctc
     1681 gtcggcgccg agccgggcgg cctgggccgt gcgctgcagg tcgccgaggc gacgggcatg
     1741 ggcgaccagc gaggtgagcg attcgccgaa ggtcggcgcc agcacttcgt cgggctggcc
     1801 gagttggtcg gcggcgtgaa ccagataggc cgccgcgcgc agctccggcg cggcgccgat
     1861 gccttcgagg atcgtcgccg tggcatcggc gtgcgcgagg acggcctcgc cggtgggcag
     1921 ggtgcgtccg gcgacgagag gcgcggcgaa ggtccgcgcg cgtccgacca gatcgctcgc
     1981 cccgccgtcg acgccttctg ccgcggcacc cgccgccggc gcatcgagca ggcggacgat
     2041 cgccgcggcg gcctcgggcg ggcgcgccga ttcggacttc atggctcgag caggaactcg
     2101 cgcaccagct cgacctggtc ggcggcgacg agcgtcggcg catggccgac accttcgatc
     2161 tcgcgcacgc gcgccttcgg gccgcgttcg gccatcgcct tcgcggtggc cgccgagagc
     2221 acgtcggaat ccctgccgcg cagcaccagc gtcgggcaac ggatcgcgtc gaacgcggcc
     2281 cagagcgccg cctctcccgc cgccgccgcg gccggcgtga cggcgcgcaa cgcgaccgcg
     2341 atcgcccggt cgtagtgaag gcgaaaccgc ccgccatcgc gacgcagcat cggacgcgtc
     2401 agcgccatcc agtcctcgcg cgagtgcggg ccgaagccgc gcgagatcgt cgacaggtaa
     2461 tcggcggctt cgtcctcgct cgcccagctg agcggcgcgc cgacatagcc ggcgatccgg
     2521 gcgatgccgg cggcgtcgat cgccgggccg acgtcgttga ggacgagccg ctcgatcggc
     2581 gtgttcggca gtccggccag ggccatgccg atcaggccgc ccatcgacgt tcccagccac
     2641 gcgacctcgg cgacgtcgag cctcgcgatc agcgtgacca tgtcggcgac gtaggccggt
     2701 acctggtagc ccatcggatc ggcgaggcga tcggattcgc cgcgcccgac gacgtcgggg
     2761 cagacgacgc gatacgagtc gcgcatcgcc cgcgccaagg tgtcgaagtc gcgaccctgg
     2821 cggccgagac cgtgcacgca gacgagcacg cgcgcgcaac gcgggtcgcc ccattcccaa
     2881 taggccatgc gatgcagccc ggccgcgccc aggcattgca cgtacttcag tcgcggcccg
     2941 gcctcggcgg gcgccgcatt ctcgttcgtc gtcatttcat ctgcaccgtg ccgttgaccg
     3001 tcgccgtgat cgtcgccttg ccgggctcga ccggcagcgg ctcgcttcgg gcgacgctcg
     3061 ccacctgcgc ccgcatcagc atcggctgcg gccggaagcc cgcgtcgttg ccggcgacgt
     3121 tcacttcgcg tatcgcatag ccggaatagc cgaactgctt cacgtaatcc tccgccttcg
     3181 cgcgaaaccg cgcgatcgcc tgggcggcga catcggcctc gaccttgcga cccgcctcgt
     3241 gcgacagccg gaagccgacg ttcgagatcg acatcgtcga gatgcgaccc gtgagcgcgc
     3301 cgatcgtcgc catgtcgcgt ccctcgacga tcaactgggc gctgccctgc cagccggaga
     3361 tcgtcgtccg cgacgagctg gcggtcgtgg catagcgcgg ccagatggag aaattgccgg
     3421 tctgcacgtc gacctcgccg gcgcgggcga ctttcctggc ctcggcgagc gccgtgtcga
     3481 gtgcctgctt gagctgcgac tgcaccgcgc cggcgtcggc gccctccttc gtcgcggcga
     3541 aggtgacgac gatgacgtcc atggcgacat cgacactggc gctgctgctg aggccgagaa
     3601 cgccctgcag tggcggagca ccctgcgcga cgccgctgag cgggaaggcg aggcaaagcg
     3661 tcgccacggc ggcgaacgag aggcgatgga gatcgaacgt catgcaaagg cccgtgagaa
     3721 ttgactcgtt ccgggttgta acagctggtc agtgctgcct tgaatcccct gcgaacccgc
     3781 gcagaatcgc gcttgttaca aatggggaag cgatgaacac gaccgccaac gcccgcccgg
     3841 accggatcgt cgtcgtcgac gatgatgccc gcatcaggga cctgctgcgc cgctacctca
     3901 cgcaggaagg attcgaggtc ctgctggccg aagacgcgaa ggcactcaac cggctgctga
     3961 cgcgcgagac cgtgcacctg atcgtgctcg acctgatgct gcccggcgag gacggcctgt
     4021 cgatctgccg
//
LOCUS       DASRWS010000011         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3457827, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000011 DASRWS010000000
VERSION     DASRWS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457827"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFF72_00160"
     CDS             complement(<1..260)
                     /locus_tag="VFF72_00160"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZV91592.1"
                     /translation="MTAISPATEADATEPAEQPAPACVMSFNAADPSGAGGSAGDVST
                     IAAMGAHALPVITSIVMRDTAEIFDHHPLEADLIVEQARSVL"
     gene            440..592
                     /locus_tag="VFF72_00165"
     CDS             440..592
                     /locus_tag="VFF72_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZV91593.1"
                     /translation="MCLICGWMYDEAAGDPEHGLAPGTVWADVPMNWTCPECGARKED
                     FEMVQI"
     gene            643..1041
                     /locus_tag="VFF72_00170"
     CDS             643..1041
                     /locus_tag="VFF72_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV91594.1"
                     /translation="MSNAAQAESAGLKVLVIDDSNTIRRSAEIFLKQGGYEVLLAEDG
                     FDALSKVNDHDPDLIFCDILMPRLDGYQTCAIIKRNQKFATVPIIMLSSKDGLFDKAR
                     GRMVGSEDYLTKPFTKDQLLHAVQQHRRAA"
     gene            1110..1475
                     /locus_tag="VFF72_00175"
     CDS             1110..1475
                     /locus_tag="VFF72_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV91595.1"
                     /translation="MPIQKILLVDDSKTELHHLAELLGKRGFEVRTAENGEDAMKRLG
                     EEKPDLILMDVVMPGQNGFQLTRAITRDPRFSGVPVIMCTSKSQETDKVWGMRQGARD
                     YIVKPVDATELMAKIRAFD"
     gene            1497..2015
                     /locus_tag="VFF72_00180"
     CDS             1497..2015
                     /locus_tag="VFF72_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZV91596.1"
                     /translation="MANKQALRDLQTRLAERLQAARTEPVARSWLAVECGARGFLLPL
                     RDAGEIFAVVPMVHVPHSHRWFLGVANLRGHLHGVVDLAGFLGVKTADTQRDQSRLVG
                     FNQSLDINCVLLVDRLAGLRGDDDLTRDSEEQGARPAFAGGLFRDASARVWQELNLAE
                     LASDESFLRIVG"
BASE COUNT          386 a          687 c          734 g          356 t
ORIGIN      
        1 tcgaggacgc ttcttgcctg ctcgacgatc agatcggctt cgagtggatg gtggtcgaag
       61 atctcggcgg tgtcgcgcat cacgatgctg gtgatcaccg gcagggcgtg cgcacccatc
      121 gcggcgatcg tcgacacatc gcccgccgag ccgccggcac cgctcggatc ggcggcgttg
      181 aagctcatga cgcaggccgg cgcgggttgc tcggcgggtt cggtggcgtc ggcctcggtg
      241 gccggtgaga tggcggtcat ggatgcgtgg aaagcggcgg gcgaagcgtt tgcgaaaggt
      301 cccggcagac ctctaggcga acgtgaagaa tgttgcgtat gtatcgacgc cgccttgctt
      361 ttcctcgagg ggccgtgtct acaatcccgc tgcattgtag tgaaggcatc gagcgcccgt
      421 gagtgaaccg aagacctgga tgtgcctgat ctgcggatgg atgtacgacg aggcggccgg
      481 cgacccggaa cacggtcttg cgccagggac ggtctgggcc gacgtgccga tgaactggac
      541 gtgtccggaa tgcggcgccc gcaaggaaga cttcgagatg gtgcagatct gagcgtcgtg
      601 ccgatgcgca ccggcgaaaa gagtcgacga ggagaacgct aggtgagcaa tgcggcccag
      661 gccgaatccg ccggactcaa ggtgctcgtc atcgacgaca gcaacacgat ccggcgcagc
      721 gcagagatct tcctgaagca gggcggctac gaggtgcttc tcgccgagga cggcttcgac
      781 gcgctctcga aggtcaacga tcacgaccct gacctgatct tctgcgacat cctgatgcct
      841 cgtctcgacg gctaccagac ctgcgcgatc atcaagcgca accagaagtt cgccacggtg
      901 ccgatcatca tgctgtcgtc gaaggacggc ctgttcgaca aggcacgcgg ccggatggtc
      961 ggctcggagg actacctcac caagccgttc acgaaagacc agctgctgca tgcggtgcag
     1021 cagcaccggc gcgccgcgtg atgcgcggcg ctcgccccgg caaggccgcg acccgtcgcg
     1081 gctagcgcaa cgtctgcaga aagcgaccca tgccgatcca gaagatcctt cttgtcgacg
     1141 actcgaagac cgagctgcac catctcgccg aactgctcgg caagcgcggg ttcgaggtcc
     1201 gcaccgccga gaacggcgag gacgcgatga agcggctcgg cgaggaaaag cccgatctga
     1261 tcctgatgga tgtcgtcatg ccgggccaga acggctttca gctgacgcgc gccatcaccc
     1321 gcgacccgcg cttctcgggc gtgccggtga tcatgtgcac gagcaagagc caggagaccg
     1381 acaaggtctg gggcatgcgc cagggcgcca gggactacat cgtcaagccg gtcgacgcga
     1441 ccgagctgat ggccaagatc cgcgccttcg actgacgccg acacggacgc gagtgaatgg
     1501 cgaacaagca ggccctgcgc gatctccaga cccggctcgc cgagcgtctc caggcggccc
     1561 gcaccgagcc ggtcgcgcgg tcgtggctgg ccgtcgaatg cggcgcgcgc gggtttctcc
     1621 tgcctctgcg cgatgccggc gagatcttcg cggtcgtgcc gatggtgcac gtgccgcaca
     1681 gccaccgctg gttcctcggc gtcgcgaacc tgcgcggcca tctgcacggc gtcgtcgacc
     1741 tggcgggctt tctcggcgtg aagacagccg acacgcagcg tgaccagtcg cggctggtcg
     1801 gcttcaacca gtcgctcgac atcaactgcg tccttctcgt cgatcgactg gccggcctgc
     1861 gcggcgacga cgatctcacc cgcgacagcg aggagcaggg agcgcgaccg gccttcgccg
     1921 gcggattgtt tcgcgatgcc tcggcgcgcg tctggcagga gctcaatctc gctgagctgg
     1981 cgagcgacga gtcgttcctg cgcatcgtcg gttagggagc tgcccggatg aaccactgct
     2041 tcgctttttt cgtcgcatcg cctcgccagg gcggcgttcc tcgcgcgatc ggtcagtcgc
     2101 cgcagggcaa cctcgagagg gccatatgag cttcgtcaac aagatcaagg gatgggggca
     2161 gaa
//
LOCUS       DASRWS010000012         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_293791, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000012 DASRWS010000000
VERSION     DASRWS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293791"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFF72_00185"
     CDS             <1..323
                     /locus_tag="VFF72_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZV91597.1"
                     /translation="EGIGLPEGIGINHRLTHFQHLFASSGYAAGYYVYLWAEVLDADG
                     FEAFVEAGNPFDGDVAKRLLRHIYSSGNSVEPGAAYRAFRGKAATPHPMLRQRGLLEE
                     ATTG"
     gene            625..1065
                     /locus_tag="VFF72_00190"
     CDS             625..1065
                     /locus_tag="VFF72_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91598.1"
                     /translation="MKAAVMAIVVFTLGLSAFDTHAARLTQDPLTRLPLPESTTIPLS
                     PNNEPTAMPTGTVCKSSFQGNHYMLFGKLDAAVAWYRGRLDGFSHLQSADGRMHVFSD
                     PARSVVIIVMGSPGGDASSVAYEVYRPGLAQGTLAGFTQNKMSC"
     gene            complement(1340..>2852)
                     /locus_tag="VFF72_00195"
     CDS             complement(1340..>2852)
                     /locus_tag="VFF72_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV91599.1"
                     /translation="YMVVNHHFERRFGLRREDVIGRTDREVFPLQAATVYEAHDRQVM
                     RTGVAMEVEEPATGLEEDGSWLSVKFTLLDPEGKPYALGGISTDITDRKRAEAAAREA
                     RDEAERANEAKSEFLSRMSHELRTPLNAVLGFTQLLQIEARHSARTDQLAKLGHIRAA
                     GDHLLALINDVLDLSGLDSGELRLPLQPVALDALVRQSVPLVESLAAQHGVSVELGEL
                     GGVVAANPTRLRQVLINLLSNAIKYNQRGGRVFVHAETDGDRSILSVRDTGRGMSRAQ
                     LDRLFEPFNRFGVENEGIEGTGIGLTIVKAIIEGMGGVISVASQPGKGTAFDVSLPAM
                     RDDCAAVAESAPEAAADIVAPMPGPRPGRILYIEDNEVNVMLVEELVRSVGGLTIVSE
                     LCGADGVARAREMRPDLVLIDLQLPDFDGFEVLRRLRADPVTRDIDCVALSANAMPED
                     IARGRAAGFADYWVKPIDFTTFLAALNARFPTSAERPAARSSCDAPAAARSMT"
BASE COUNT          434 a          968 c          996 g          454 t
ORIGIN      
        1 tcgaaggcat cggcctgccc gagggcatcg gcatcaacca ccggctgacg cacttccagc
       61 acctcttcgc cagctcgggc tacgccgccg gctactacgt ctacctctgg gccgaggtgc
      121 tcgacgccga tggcttcgaa gccttcgtcg aagccggcaa cccgttcgac ggggacgtcg
      181 cgaagcgcct gctgcgccac atctactcga gtggcaattc ggtcgaaccg ggcgccgcgt
      241 accgggcctt tcgcggcaag gcggcgacgc ctcaccccat gctgcgccag cgcggactgc
      301 tggaagaggc cacgacaggc tgagccggcc gcgctgaaag cggggagggc ggtcgaaccc
      361 tcgacgactt cacatctggc cggaaaaggc gcgcgctctc gtctcggttc ggtgatcgat
      421 gtgcgcgcga cggttcggcc atgcgacgga ggagtcgacg ccgaccgctc gtcgacaggt
      481 ctcgctgccg tctcatggcc tggaagccgc gttgtctgaa gccgcccgga gtcggcccgt
      541 tcggcgccac ggcgggacga tggcggggtt cgagcatccg tcctgttctt tccgaggtca
      601 tcactcgact tcgaaaggat cgtcatgaaa gccgccgtca tggcaatcgt cgtcttcacg
      661 ctcggcttgt cagccttcga cacgcacgct gcgcgactga cgcaagaccc gctcaccagg
      721 ctgccccttc cagaaagcac cacgattccc ctgtctccga acaacgagcc gacggcgatg
      781 cccacgggca ccgtctgcaa gagcagcttt cagggcaacc actacatgct gttcggcaag
      841 ctcgacgcgg ccgtggcgtg gtatcggggt cgtctcgacg gcttttcgca tcttcagtcg
      901 gccgacgggc gaatgcatgt gttcagcgac ccggcgcgtt ccgtcgtcat catcgtcatg
      961 ggctcaccgg ggggcgatgc gagctcggtc gcctacgagg tgtaccggcc ggggctcgcg
     1021 cagggcacgc tggcgggctt cacccagaac aagatgagct gttgagccgc acccgccgtc
     1081 ggcgcatggc tcgcggcgtg ctcgagctgg gccgtcgttt ctgcggtcgt cttacgagcg
     1141 ctcgctattt ccagtcgcgt cgcggacagc actgcgccat ctgcagggac accccggaaa
     1201 tgcaggaacc gcgtcggtcg caggcctgag cattcgcctg ggccgacccc gcttcgcggc
     1261 gcggctccgt ccaggcgccg gcgcccgatc gaagtgacgc aattcgaaga cgacggtgca
     1321 ttccgcgggc cggacgggat caggtcatcg accgtgccgc ggccggggcg tcgcacgacg
     1381 acctggcggc gggccgctcg gcacttgtcg gaaagcgcgc attgagcgcc gccagaaacg
     1441 tcgtgaagtc gatcggcttg acccagtagt cggcgaagcc ggcggcgcgg ccgcgggcga
     1501 tgtcctcggg catggcgttg gccgacaggg cgacgcagtc gatgtcgcga gtcaccgggt
     1561 cggcgcgcag ccggcgcaac acctcgaagc cgtcgaagtc gggaagctgc aggtcgatga
     1621 ggacgagatc ggggcgcatc tcgcgcgccc tggcgacgcc gtcggcgccg cacagctcgc
     1681 tgacgatcgt caggccgccg acgctgcgca cgagctcctc gaccagcatg acgttgacct
     1741 cgttgtcctc gatgtagagg atgcggccgg gccgcggccc gggcatcggg gcgacgatgt
     1801 cggccgcagc ctccggcgcc gactccgcga cggcagcgca gtcgtcgcgc atcgccggca
     1861 ggctgacgtc gaacgcggtg cccttgcccg gctggctcgc caccgagatc acgccgccca
     1921 tgccctcgat gatggccttg acgatcgtca ggccgatccc ggttccctcg atgccttcgt
     1981 tctcgacgcc aaagcggttg aaaggctcga acaggcggtc gagctgggcc cgcgacatgc
     2041 cgcgcccggt gtcgcgcacg ctcagaatcg aacgatcgcc gtcggtctcg gcgtggacga
     2101 agacgcgccc gccgcgttgg ttgtacttga tggcgttcga cagcaggttg atgaggacct
     2161 ggcgaagccg cgtcggattg gcggcgacga cgccgccgag ctcgcccagc tcgaccgaga
     2221 cgccatgctg cgcagcgagc gactccacca gcggcaccga ttggcggacc agtgcgtcga
     2281 gcgccaccgg ctgcagcggg aggcgcagct cgcccgagtc gaggccggag agatcgagca
     2341 cgtcgttgat gagcgcgaga aggtggtcgc cggcggctcg gatgtggccg agcttggcga
     2401 gctggtcggt gcgcgcggag tggcgcgcct cgatctgcag cagctgggtg aagccgagaa
     2461 cggcgttgag cggcgtgcgc agctcgtggc tcatgcgcga gaggaactcg ctcttggcct
     2521 cgttcgcgcg ctcagcctcg tcgcgcgcct cgcgcgccgc ggcctcggcc cgcttgcggt
     2581 cggtgatgtc ggtcgagatg ccgcccagcg cgtagggctt gccctcgggg tcgagcagcg
     2641 tgaacttgac cgacagccag ctgccgtcct cctccaggcc ggtcgcgggc tcctcgacct
     2701 ccatcgcgac gcccgtgcgc atgacttggc ggtcgtgcgc ctcgtagacg gtggcggctt
     2761 gcagcgggaa gacctcgcgg tcggtgcggc cgatgacgtc ctcgcgacgc aacccgaagc
     2821 ggcgctcgaa gtggtggttg acgaccatgt ag
//
LOCUS       DASRWS010000013         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2020614, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000013 DASRWS010000000
VERSION     DASRWS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020614"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VFF72_00200"
     CDS             complement(<1..163)
                     /locus_tag="VFF72_00200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426883.1"
                     /note="lipoyl/octanoyltransferase; catalyzes the transfer
                     of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto
                     lipoate-dependent enzymes like pyruvate dehydrogenase and
                     the glycine cleavage system H protein; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase"
                     /protein_id="HZV91600.1"
                     /translation="MRAFTASRNDSTPDELWLCEHPPVYTQGLAGRAEHVRDAGSIPV
                     IATDRGGQVT"
     gene            complement(227..514)
                     /locus_tag="VFF72_00205"
     CDS             complement(227..514)
                     /locus_tag="VFF72_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HZV91601.1"
                     /translation="MESLMQDIPAEQSLIEYPSRFPIKVMGVNADGFVHAVTEIARRF
                     DPGFDAATIELRPSSGSKYLGVTVTVNATSRAQLDELYRTLSTHPMVKVVL"
     gene            complement(565..1461)
                     /locus_tag="VFF72_00210"
     CDS             complement(565..1461)
                     /locus_tag="VFF72_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HZV91602.1"
                     /translation="MHQTTLPDTLCYLDGEYLPLSQAKVSVLDRGFIFGDGVYEVMPV
                     YDRRLFRFDEHLARLGRSLSKLRMPSPHSREQWLERCRRLIAALHEHGGAVDQTVYVQ
                     VTRGVAASRDHVMPPGLVPTVFMMANPMKPASGEQRHHGVACISARDFRWERGDIKSV
                     SLLGNVLARQMSADHDALETIMFRDGYLTEASGSNVWVVHEGAVLGPPKSEHVLEGIR
                     VELIKELCDECGIAYNLRPIAEADVASADELLLSSATKEVLPVTKLDGEGVGHGALRG
                     KPGPVYARLYEAYQRAKRKQSI"
     gene            complement(1488..2675)
                     /locus_tag="VFF72_00215"
     CDS             complement(1488..2675)
                     /locus_tag="VFF72_00215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZV91603.1"
                     /translation="MPIFRFAAARLLSLCLVLPPGVALAQVPQPPEVAAKSYIVLDLT
                     THQTLAERNADAPADPASLTKLMTAYVVFQALREKKLALQQTLPVSRRAWDERKGGGS
                     LMFIDTTMHPTVDDLLRGLIAVSGNDAAVALAEGVAGSVDNFVVMMNRQAQAMGLKNT
                     SFKNVTGLTEAGHKSSARDLAIVAEHIITDFPEYFPYYAIKEFKYDNIAQPNRNLLLR
                     RDPSVDGMKTGYTEAAGYCLLATAQREFPNLGPSGGAGKRRIMTVVLNTTSMEARANE
                     SQKLLNWAFSAFDTVRLFDDGKAIVTTPVWKGTQADIKLGAGKAVFVSVPKGEGGRLQ
                     TQVERTDPLVAPLAKGQRVGTIRVSTSAGAPVVALPLVVLEPVEMAGVFGRAWDAVRL
                     WIK"
     gene            complement(2707..>3211)
                     /locus_tag="VFF72_00220"
     CDS             complement(2707..>3211)
                     /locus_tag="VFF72_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV91604.1"
                     /translation="DNKVAQTLARAFVEVGWASVRFNFRGVGESEGSWDDGRGEVEDA
                     LAVIHETRAKVDFAGLPLALAGFSFGGFVAAAAAERLADADKPRRMVLVAPSTQKQDV
                     PAVPQGTIVVQGDADELVPLESTLAWARPQDLPVIVFPGVGHFFHGRIALLKGIVVRE
                     LRGLDAI"
BASE COUNT          500 a         1096 c         1089 g          526 t
ORIGIN      
        1 aggtgacctg gcctccgcga tcggtggcga tgacgggaat gctgcccgca tcgcgcacgt
       61 gttccgcgcg ccctgccagg ccttgcgtgt acaccggcgg gtgttcgcac agccaaagct
      121 cgtcgggcgt gctgtcgttg cgcgacgcgg tgaaagcccg catggcctcg taggtgggca
      181 ggtactccac ccggccgcgc aggatctgct gcacctgtgt cccgcgctag agcacgacct
      241 tgaccatcgg atgcgtcgac agcgtgcggt agagctcgtc gagctgcgcc cggctcgtcg
      301 cattcacggt gaccgtgacg ccgagatact tcgagccgct gctcggccgc agttcgatcg
      361 tcgccgcatc gaagccgggg tcgaagcgcc gcgcgatttc ggtcacggcg tggacgaagc
      421 cgtccgcatt gacgcccatc accttgatcg ggaagcgcga cggatattcg atcagcgact
      481 gttcggcggg gatgtcctgc atgagtgatt ccatcgtcgc catctggggc cgccgggcgc
      541 cattgcaacg cgaggccgac aggctcagat cgactgcttg cgcttggccc gctggtaggc
      601 ctcgtagaga cgcgcgtaga ccggcccggg cttgccgcgc aacgcgccgt ggccgacgcc
      661 ttcgccatcc agcttcgtca ccggcagtac ttccttcgtc gccgagctga gcagcaactc
      721 gtcggccgag gcgacgtcgg cctcggcgat cggcctgagg ttgtaggcga tgccgcactc
      781 gtcgcacagc tccttgatca gctcgacccg gatcccctcc agcacgtgct cgctcttcgg
      841 cggcccgagc accgcccctt cgtgcacgac ccagacgttc gacccggacg cctcggtcag
      901 gtagccgtcg cgaaacatga tcgtctccag ggcatcgtgg tcggccgaca tctgccgggc
      961 gaggacattg cccagcaagg agacgctctt gatgtcgccg cgctcccagc ggaaatcacg
     1021 cgcgctgatg caggcgacgc catgatggcg ctgctcgccg ctggccggct tcatcgggtt
     1081 ggccatcatg aacaccgtcg gcacgagccc cggcggcatg acgtggtcgc ggctcgcggc
     1141 gacgccgcgg gtcacctgga catacacggt ctggtcgacc gcgccgccgt gctcgtgcag
     1201 cgccgcgatc agccgccggc agcgctcgag ccactgctcg cgactgtgcg ggctcggcat
     1261 ccgcagcttc gacaggctgc ggccgagccg agccaggtgc tcatcgaagc gaaagaggcg
     1321 gcgatcgtag acgggcatca cttcgtagac cccgtcgccg aagatgaagc cccggtcgag
     1381 cacggacacc ttggcctgcg acagcggcag gtattcgccg tcgagatagc aaagcgtgtc
     1441 gggaagggtc gtctgatgca tcggtgcctc cgctggcggc cagcttacta cttgatccag
     1501 agccggaccg cgtcccacgc gcggccgaag acgccggcca tctcgaccgg ctcgaggacg
     1561 accagcggca gggcgacgac cggtgcgccc gcggacgtgc tgaccctgat cgtgccgacc
     1621 cgctggccct tggcgagcgg agcgaccagc gggtcggtgc gctcgacctg ggtctgcaac
     1681 cggccgccct cgcccttggg aacgctcacg aaaaccgcct tgccggcccc cagcttgatg
     1741 tcggcctgcg tgcccttcca gaccggcgtc gtcacgatgg ccttgccgtc gtcgaacagt
     1801 cgcaccgtat cgaacgccga gaaggcccag ttcagcagct tctggctctc gttggcgcgc
     1861 gcctccatcg aggtcgtgtt cagcaccacg gtcatgatcc gccgcttgcc ggcgccgcct
     1921 gacgggccga ggttggggaa ctcgcgctgc gccgtggcga gcagacagta gcctgccgct
     1981 tctgtgtacc ccgtcttcat tccatcgacc gaaggatcgc ggcgaagcag caggttgcga
     2041 ttcggctggg cgatgttgtc gtacttgaac tccttgatgg cgtagtacgg aaagtattcg
     2101 gggaagtcgg tgatgatgtg ctcggcgacg atcgccaggt ctcgcgccga gctcttgtgg
     2161 ccggcctcgg tcaggccggt gacgttcttg aacgacgtgt tcttcaggcc catcgcctgg
     2221 gcctgccggt tcatcatcac gacgaagttg tcgacgctgc cggcgactcc ctcggcaaga
     2281 gcgaccgccg cgtcgttgcc gctcaccgcg atcaggccac gcagcaggtc gtcgacggtc
     2341 gggtgcatcg tcgtgtcgat gaacatcagc gagccgccgc ctttgcgctc gtcccaggcg
     2401 cgcctggaca ccggcagcgt ctgctgcagc gccagcttct tctcgcgcag cgcctggaag
     2461 acgacgtagg ccgtcatcag cttggtcagc gatgccgggt cggccggcgc atcggcgttg
     2521 cgctcggcca gggtctggtg ggtcgtcagg tcgagcacga tgtagctctt ggcggcgacc
     2581 tcgggcggct gggggacctg cgccagggcg acacccggag gcaggacgag acagagcgag
     2641 agcaggcgcg ctgcggcgaa tcgaaagatc ggcatgcgag gacgttgcga tcgggaaggg
     2701 aacggctcag atcgcatcga ggccgcgcag ttcacgcacg acgatgccct tcaagagtgc
     2761 gatgcggccg tggaagaagt ggcccacgcc gggaaacacg atcacgggaa gatcctgcgg
     2821 ccgcgcccag gccagggtcg actccagcgg cacgagctcg tcggcatcgc cctggacgac
     2881 aatcgtgccc tgcggcaccg cgggcacgtc ctgcttctgc gtcgacgggg ccacgagcac
     2941 catgcggcgc ggcttgtcgg cgtcggccag ccgttcggcc gccgccgccg cgacgaagcc
     3001 gccgaacgag aagccggcca gcgccagagg cagtccggcg aaatcgacct tcgcccgcgt
     3061 ttcgtggatg accgcgaggg cgtcttcgac ctcgccgcgg ccgtcgtccc agctgccctc
     3121 ggactcgccg acgccccgga aattgaagcg gacgctggcc cagccgactt cgacgaacgc
     3181 gcgggccagc gtctgcgcga ccttgttgtc g
//
LOCUS       DASRWS010000014         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2308198, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000014 DASRWS010000000
VERSION     DASRWS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308198"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFF72_00225"
     CDS             <1..341
                     /locus_tag="VFF72_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZV91605.1"
                     /translation="SPVEGAELAAAVASLATGGGFDVLGGLQNFARLDRLALGGDSAS
                     GVTVSGGKYIGKNVYLELTGGGRAGPSAQVEVRVRNGFSIISQVGGEVGTKVGIRWRR
                     DYGAATQPKK"
     gene            complement(347..1462)
                     /locus_tag="VFF72_00230"
     CDS             complement(347..1462)
                     /locus_tag="VFF72_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV91606.1"
                     /translation="MPMVVDCDSHVMEPADLWLTHLEPKYRDRAIRIERRDGVEHLVI
                     GEESVLAGMLAGLGGAHLDRKNLFTPGMTYADGCEPASYEPGARKAMLDDWKVDVGVL
                     FPTIGILPFPTEDVELASAYCRAYNTWQMEFHQGAPGRVAPIAAVNWRDVDGAVAELK
                     RCVKAGFRGLFVPPEVVDGRRPGEAHFDPIWRVCEDADIPGCLHVIVRFTGGGGAFGQ
                     WHMTRPGSVFSFGMGATGQLIPAMASLVTDMVFERFPRLKIVSVEAGCGYAAYLMDRL
                     DEKHDILRAMLPTPMPLKPSDYIRRNCWFVAEPEERTIGAMLDLVGEDKILWGSDYPH
                     IDSTLDAPKLIRQSIADLSPERQAAVLGENARRVFGL"
     gene            complement(1540..>2325)
                     /locus_tag="VFF72_00235"
     CDS             complement(1540..>2325)
                     /locus_tag="VFF72_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV91607.1"
                     /translation="IGDAMNKASLHIVKGRIYRTGMRELVVGRTLYDQYRGVDVGDQV
                     LIHGAPWTVVGEYVDQGGIDEDSLAGDVDMVRTDLGSATYQSVGVMLASPGDFQAFSH
                     ALMNNPQLHVRVRRLSEYYRGQMGNLYALFDIVGFFVGGVMAVGAAAGALTTLYAAVD
                     ARRREIATLRAIGFDGAAVVLSIMAEALLLAVPGALIGLAVAGLGFGGHGVATANIVF
                     QATMTPQVAAIGAATAIAIGLVGGAFPALRAARVEVAEALRVT"
BASE COUNT          357 a          806 c          818 g          344 t
ORIGIN      
        1 tgtcgccggt ggagggcgcg gagctggcgg ctgcggtggc gtctctggcc accggcggcg
       61 gcttcgatgt gctgggcggg ctgcagaact tcgcccgtct cgaccggctc gcgctcggcg
      121 gcgattccgc ctccggcgtc accgtctccg gcggcaagta catcggcaag aacgtctatc
      181 tggagctgac cggcggcggc cgcgccggcc cgtcggcgca ggtcgaagtc cgcgtgcgca
      241 acggcttttc gatcatctcg caggtgggcg gcgaggtcgg gaccaaggtc ggcattcgct
      301 ggcggcgcga ctacggcgcg gcgacccagc cgaagaagtg accggcctag aggccgaaca
      361 cccgccgggc attttcgccg agcacggccg cctgccgctc cggcgagaga tcggcgatcg
      421 actggcggat caacttcggg gcgtcgagcg tcgagtcgat gtgcggatag tcgctgcccc
      481 agaggatctt gtcctcgccg accagatcga gcatcgcgcc gatggtccgc tcctccggct
      541 cggccacgaa ccagcagttg cggcggatgt agtcgctggg cttcagcggc atcggcgtcg
      601 gcagcatggc gcgcaggatg tcgtgcttct cgtcgagccg gtccatcaga taggccgcat
      661 agccgcagcc ggcctcgacg ctgacgatct tcagcctggg aaagcgctcg aagaccatgt
      721 cggtcacgag gctggccatc gccggaatga gctggccggt cgcgcccatg ccaaaggaga
      781 agacgctgcc cggccgggtc atgtgccact ggccgaacgc cccgccgcca ccggtgaagc
      841 ggacgatgac gtgcaggcag ccggggatgt cggcgtcctc gcacacccgc cagatcggat
      901 cgaagtgggc ctcgcccggg cggcgaccgt cgacgacctc cggcggaacg aacaggcctc
      961 ggaaaccggc cttcacgcag cgcttcagct cggcgaccgc gccgtcgaca tcgcgccaat
     1021 tgacggcggc gatcggcgct acccgtcctg gcgcgccctg atggaattcc atctgccagg
     1081 tgttgtaggc gcggcagtag gccgacgcga gttcaacgtc ctcggtcggg aagggcagga
     1141 tgccgatggt cgggaacagc acgccgacgt cgaccttcca gtcgtcgagc atcgccttgc
     1201 gggcgccggg ctcgtagctc gccggctcgc agccgtcggc ataggtcatg cccggcgtga
     1261 acaggttctt gcggtcgagg tgggcgccgc cgagcccggc gagcatgccg gcgagcacgc
     1321 tctcctcgcc gatgaccagg tgctcgaccc cgtcgcgccg ctcgatccgg atcgcccggt
     1381 cgcggtactt gggctccaga tgcgtgagcc acagatccgc cggctccatc acgtgggaat
     1441 cgcagtcgac gaccatcggc atggtgcggc cctacctctg tcagaacgcc aggatgagca
     1501 caggcgccgt tacgtgcgtc aaccgcctgc ggcgttctgt caggtcaccc tgagggcttc
     1561 ggcgacctcg acgcgtgcgg cgcggagggc cgggaacgcg ccgccgacga ggccgatcgc
     1621 gatcgccgtc gccgcgccga tggccgcgac ctgcggcgtc atcgtcgcct ggaagacgat
     1681 gttggccgtg gcgacgccgt gtccgccgaa gccgagcccc gcgaccgcca ggccgatgag
     1741 cgcgcccggc acggccagca gcagcgcctc ggccatgatc gagaggacga cggcggcgcc
     1801 gtcgaagccg atcgcgcgca gggtggcgat ctcgcgccgc cgggcgtcga ccgccgcata
     1861 gagggtcgtc agtgcgccgg ccgcagcgcc gaccgccatc accccgccga cgaagaatcc
     1921 gacgatgtcg aacagcgcgt agagattgcc catctggccg cggtagtact cggagagccg
     1981 ccgcacccgc acgtgcagct gcgggttgtt catcagcgcg tggctgaagg cctggaagtc
     2041 gccgggcgac gccagcatga cgccgacgct ctggtaggtc gccgaaccca ggtccgtgcg
     2101 caccatgtcg acgtcgccgg cgaggctgtc ctcgtcgatc ccgccctgat cgacgtactc
     2161 gccgaccacc gtccatggcg ccccgtggat gagcacctga tcgccgacgt cgacgccgcg
     2221 gtactggtcg tagagggtgc ggccgacaac cagctcgcgc atgccggtgc gatagatgcg
     2281 ccccttcacg atgtgcagcg aggccttgtt catagcgtcg ccgat
//
LOCUS       DASRWS010000015         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4034628, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000015 DASRWS010000000
VERSION     DASRWS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4034628"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFF72_00240"
     CDS             complement(<1..418)
                     /locus_tag="VFF72_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HZV91608.1"
                     /translation="MTAIRLHIDGIGFWAPTLPGWTEARAALRGEAGLLEPPSRRPAP
                     EILAPAERRRAPDSVALALEVAAAATHDAGCDGACLPSVFTSAHGDLAVNDYMCTTLA
                     SQPTALSPTRFHNSVHNASAGYWTIGTGCMAASTALT"
     gene            complement(415..1620)
                     /locus_tag="VFF72_00245"
     CDS             complement(415..1620)
                     /locus_tag="VFF72_00245"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HZV91609.1"
                     /translation="MMQRIAPTSITAWTTTTAAGHGNGALLAALRARRSALTVNDFGP
                     APLATWVGRVAALESTPLPRALADWDCRNNRLAWLGLGADGFIDAAHAARERHGTARV
                     ALVLGTSTSSIGATEDAYRHLDEEGRFPAGNRNPLVHTPHSLGRFVQEALALEGPCVT
                     VSTACSSSAKVFAAAERMLRLGLADAAIVGGVDTLCGSVLFGFNALQLVSPTPCRPFD
                     AGRDGISLGEAAGFALLERGPRGDAPQLLGYGESSDAHHMSTPHPGGLGAERALDDAL
                     ARAGLEAAQVDHVNLHGTASEKNDDVEAALVARRFPARTHASSTKGCTGHTLGAAGIV
                     EAAATLLAIETGFMPGTVNTEHLDPACGPQIRLEGATGDVRVAASNSFGFGGNNCVLV
                     FGRAGGVRP"
     gene            1712..2779
                     /locus_tag="VFF72_00250"
     CDS             1712..2779
                     /locus_tag="VFF72_00250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZV91610.1"
                     /translation="MSSRGSERAIGWVEQGLVPDRVVRLGIRRLLRERLQEIGDGDAE
                     AASRIAESFVASLARSEVAIATDKANEQHYEVPAEFFGRVLGPHRKYSCCEWPDGGTS
                     TLADAEAAALSTTCERAGIADGQRILELGCGWGSLTLWMAERYPKSTITALSNSSSQR
                     VFIESEAARRGLANVRVVTEDFNTFASKARFDRVVSVEMFEHLRNWPEAFHRVAGWLA
                     PGGRFFMHVFVHRSTPYAFVDRDASDWMSRHFFSGGMMPSDDLAVRFQDDLRLVGRWR
                     WNGEHYAKTAEAWLANMDANRLVLWPLFETLYGSDQAATWWVRWRLFFLSCAELFGHD
                     GGQCWRVAHYLFESRRAGAAS"
     gene            2776..>3085
                     /locus_tag="VFF72_00255"
     CDS             2776..>3085
                     /locus_tag="VFF72_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HZV91611.1"
                     /translation="MTPTVEAALLGLVAALALALCIWVASLVHHDVSLVDRVWSLLVA
                     VPAVAYAWVLPPGSDARFTVMAVVLVVWAARLAVYVTWRNWGHGEDRRYQAIRARNQP
                     N"
BASE COUNT          412 a         1066 c         1148 g          459 t
ORIGIN      
        1 cggtcagcgc cgtgctcgcc gccatgcagc cggtgcctat cgtccagtag ccggccgagg
       61 cgttgtgcac cgagttgtga aagcgcgtcg gcgaaagcgc cgtcggctgg ctcgccagcg
      121 tcgtgcacat gtagtcgttg accgcgaggt cgccatgtgc cgacgtgaag accgaaggca
      181 ggcaggcgcc gtcgcagccg gcgtcgtgcg tcgccgcggc ggcgacctcg agggccaggg
      241 cgacgctgtc gggggcgcgc cgccgctcgg ccggcgccag gatctcgggc gccgggcgcc
      301 tggacggtgg ctcgagaagg ccggcctcgc cgcgcagcgc ggcgcgcgcc tcggtccagc
      361 cgggcagcgt cggcgcccag aagccgatgc cgtcgatgtg caggcgaatc gcggtcacgg
      421 ccgaaccccg ccggcgcgac cgaagacgag cacgcagttg ttgccgccga agccgaacga
      481 gttgctggct gcgacgcgga catcgccggt cgccccttcg aggcggatct gcggcccgca
      541 tgcgggatcg agatgctcgg tgttgacggt gcccggcatg aagcccgtct cgatcgccag
      601 gagcgtcgcc gcggcctcga cgatgccggc ggcgccgagc gtgtgccccg tgcaaccctt
      661 ggtcgagctg gcgtgcgtgc gcgccggaaa gcggcgggcg accagcgcgg cctcgacgtc
      721 gtcgttcttc tcgctggccg tgccgtgcag gttgacgtga tcgacctgcg ccgcctcgag
      781 gccggcgcgg gccagcgcgt cgtcgagcgc gcgctcggcg ccgaggccgc ccggatgcgg
      841 cgtcgacatg tggtgcgcgt cgctcgactc gccgtagccg agcagttgcg gcgcatcgcc
      901 tcgcgggccc cgctcgagca gcgcgaagcc ggccgcctcg cccaggctga tgccgtcgcg
      961 gccggcgtcg aacggccggc acggcgtcgg cgagacgagc tgcagggcgt tgaagccgaa
     1021 cagcacgctg ccgcacagcg tatcgacgcc gccgacgatg gccgcgtcgg ccaggccgag
     1081 ccgcagcatc cgctcggccg cggcgaagac cttggcgctc gacgagcacg cggtcgagac
     1141 cgtcacgcaa ggaccttcga gcgcgagtgc ttcctgcacg aagcggccca gcgaatgcgg
     1201 cgtgtgcacg agcgggttgc gattgccggc gggaaagcgc ccctcctcgt cgaggtgacg
     1261 gtaggcgtcc tcggtggcgc cgatgctcga cgtcgaggtg ccgaggacga gggcgacacg
     1321 cgccgtgccg tgacgttcac gggccgcgtg ggcggcgtcg atgaagccgt cggcgccgag
     1381 tccgagccag gcgaggcggt tgttgcggca gtcccagtcg gcaagcgccc ggggcagggg
     1441 cgtcgactcg agcgcggcca cgcggccgac ccaggtcgcc agcggcgccg gcccgaagtc
     1501 gttcacggtc aacgcgctgc gccgcgcccg cagcgcggcg agcagcgcgc cgttgccgtg
     1561 tccggctgca gtcgtcgtcg tccaggcggt gatcgaagtc ggggcgatgc gctgcatcac
     1621 gggggctttg ggcagaatga tcgattatcc cgtctgaatc cgacggcctt gccgtcgcgt
     1681 acagatcgcg atcacccggc cggagaggcg catgagttca cgaggcagcg agcgcgcgat
     1741 cggctgggtc gagcagggac tggtgcccga tcgggtcgtt cgcctcggca tccggcgcct
     1801 gctcagggag cggctgcagg agatcggcga cggcgacgcc gaagccgcgt cgcggatcgc
     1861 cgagtcgttc gtcgcctcgc tggcgaggtc cgaggtggcg atcgccaccg acaaggccaa
     1921 cgagcagcat tacgaagtgc cggcggagtt cttcggccgc gttctcgggc cgcaccgcaa
     1981 gtacagctgc tgcgaatggc cggacggcgg cacgtcgacg ctggccgacg ccgaggcggc
     2041 ggcactgtcg acgacctgcg agcgcgccgg catcgccgac ggccagcgca tcctcgagct
     2101 cggctgcggc tgggggtcgc tgacgctgtg gatggccgag cgctatccga aaagcacgat
     2161 caccgcgttg tcgaactcga gctcgcagcg cgtcttcatc gagagcgagg cggcgcggcg
     2221 cggtctcgcc aacgtccggg tcgtgaccga ggacttcaac accttcgcgt cgaaggcgcg
     2281 gttcgatcgc gtcgtctcgg tcgagatgtt cgagcatctt cgcaactggc ccgaggcatt
     2341 tcatcgggtc gccggatggc tcgcgcccgg cgggcggttc ttcatgcacg tcttcgttca
     2401 tcgatcgacg ccctacgcgt tcgtcgatcg cgacgccagc gactggatga gccggcattt
     2461 cttctcgggc ggcatgatgc cgagcgacga cctcgccgtg cgcttccagg acgacctgcg
     2521 cctcgtcggg cgctggcgct ggaacggcga gcactacgcg aagacggcgg aggcgtggct
     2581 cgccaacatg gacgcgaacc ggctcgtcct gtggccgctc ttcgaaaccc tctacggcag
     2641 cgaccaggcg gcgacctggt gggtgcgctg gcgcctcttc ttcctttcct gcgccgagct
     2701 gttcggccac gatggcggcc agtgctggcg tgtcgcgcac taccttttcg aatcgcgccg
     2761 cgccggcgcc gcgtcgtgac gccgaccgtc gaggcggctc ttctcggcct cgtcgcggcg
     2821 ctcgcgctgg ccctgtgcat ctgggtcgcg agcctggtcc accacgacgt cagcctggtc
     2881 gaccgggtct ggtcgctgct cgtcgccgtt ccggcggtgg cgtatgcgtg ggtcctgccg
     2941 ccgggcagcg acgcccgctt caccgtcatg gccgtcgtcc tcgtcgtctg ggcggcgcga
     3001 ctggcggtct acgtgacctg gcgcaactgg ggccacggcg aggaccggcg ctaccaggcg
     3061 atccgtgcgc gcaaccagcc gaact
//
LOCUS       DASRWS010000016         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1446338, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000016 DASRWS010000000
VERSION     DASRWS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446338"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFF72_00260"
     CDS             <1..485
                     /locus_tag="VFF72_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91612.1"
                     /translation="LDADALKRWFGSRRPTAKRYHDAIVEGRGFERLQSVADLRPGDL
                     IAIEYLTRHDNTGHVMLVAGAARQRPASPPLVDGTGQWSVDVIDSSESGHGATDTRHK
                     RGPDGHDHDGLGGGVLRLYTDAAGHVEGFSWSTLKGSHFVAPADEHVVLGRFLPGFRP
                     "
     gene            526..1638
                     /locus_tag="VFF72_00265"
     CDS             526..1638
                     /locus_tag="VFF72_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZV91613.1"
                     /translation="MTTKPPTIDDLRRYAVARTLFRPTTLGRAIDRLGFVQADPIRAP
                     ARAQDLILRHRVTAYRAGDLERRYARLPVEEDFVVNYGFVSRRHLAVLHPRTARRAWT
                     AATQRQAAEVLEFIRERGATHPRDVEARFAHGRVTNWFGGTSNAATQLLDGMHYRGLL
                     RVRRREGGTRVYEAIEHAAPAETASMRAARAEALLDLVVRLYAPLPSASLGYLTSLLI
                     NGSPQLRTELKAGLALARTRLPGVRIDGTHWLWPADENPRSRRHAVDDRVRLLAPFDP
                     IVWDRRRFALLWGWTYRFEAYVPPGQRRLGYYALPILHGEHVVGWCNASVREGRLRLD
                     SGFADGRRRDTAFRTELAAEHERLGEFLGVAESLSR"
     gene            1880..3130
                     /gene="kch"
                     /locus_tag="VFF72_00270"
     CDS             1880..3130
                     /gene="kch"
                     /locus_tag="VFF72_00270"
                     /inference="COORDINATES: protein motif:HMM:NF007828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HZV91614.1"
                     /translation="MIFNRLPAPLRASTRRVSQALGLPYWFPHAPLALLVALGGFLLL
                     DGDFGQTWHRYLDEFAHGSFSLGPRLLPPLLLGVGMLTMALGLLLRSRVAWTMALLLV
                     GTAAVSTRFGSHAGGFALLGYFVALLAALLIAWRHFDRSSVAASTLFALTSVAMLLMY
                     ATFGSYYLGTSYKPPITDLVTALYYAMVTMSTVGYGDIVPQSAEAKLFTVSIIVLGVA
                     VFATSLTAVIAPMVSRSLLRIVDRKGKTMTREDHFVVVGSTPLADNTWRELARRGCPV
                     TRILREAPGGDADDETDTVIGDASRTDVLKQAGADRATAVLAMMADDSENAFIVLAVK
                     ELGGGAQTIAAVNDSRHLSRIKLVQPDVVIAPQVLGAELAAMLLSGETVTPEFVMQRV
                     FQREVPAAATSPGSAPPASGKPRE"
BASE COUNT          474 a         1197 c         1108 g          498 t
ORIGIN      
        1 gcctcgacgc cgacgcgctg aagcgctggt tcggcagcag gcgaccgacc gcgaagcgct
       61 accacgacgc catcgtcgaa ggtcgcggct tcgagcggct gcagtccgta gccgacctga
      121 ggccgggcga cctcatcgcc atcgaatacc tgacccggca cgacaacacc ggccacgtca
      181 tgctcgtcgc cggagcggcc aggcagcggc cggcgtcgcc gccgctcgtc gacggtaccg
      241 ggcagtggtc ggtcgacgtc atcgacagct ccgaatcggg ccacggcgcg accgacacgc
      301 ggcacaagcg cggccccgac ggccacgatc acgacggcct cggcggcggc gtccttcgcc
      361 tctacacgga tgcggcaggg cacgtcgaag gcttcagctg gagcacgctg aagggctcgc
      421 acttcgtggc accggccgac gagcacgtgg tcctcggccg tttcctgccg ggctttcggc
      481 cctgagcagg gctcgatcgc atcggcgtcg cggcatcatc gccgcatgac gacgaagccg
      541 ccgacgatcg acgacctgcg ccgctacgcg gtggcacgca cgctgttcag gccgacgacg
      601 ctcggtcggg ccatcgaccg tctcggcttc gtccaggccg atccgatccg cgcaccggca
      661 cgcgcgcagg acctgatcct gcgccaccgc gtcaccgcct accgcgccgg cgatctcgag
      721 cgtcgctacg cgcggctgcc ggtcgaggaa gacttcgtcg tcaactacgg cttcgtctcg
      781 aggcgccatc tcgccgtcct gcacccgcgc acggcgcggc gggcatggac cgcggcgacg
      841 cagcgccagg cggccgaggt cctcgagttc atccgcgagc gcggcgccac gcacccgcgc
      901 gacgtggaag cccgcttcgc ccacggccgc gtgacgaact ggttcggcgg caccagcaac
      961 gcggccacgc aactcctcga cggcatgcac taccgggggc tgctgcgcgt gcggcgacgc
     1021 gaaggcggca cccgggtgta cgaggcgatc gaacatgcgg cgccggccga gacggcgtcg
     1081 atgcgggccg cgcgcgccga ggccctgctc gatctcgtgg tgcgcctcta cgcgccgctg
     1141 ccgtcggcca gtctcggcta cctgacctcg ctgctcatca acggctcgcc gcagctgcgc
     1201 accgagctga aggccgggct ggctctggcc cgcacgcggt tgccgggcgt gcgcatcgac
     1261 ggcacgcact ggctctggcc agcagacgaa aatccccgct cgcgacgcca tgccgtcgac
     1321 gatcgcgtgc gcctgctggc gcccttcgac ccgatcgtct gggaccgccg gcgcttcgca
     1381 ttgctctggg gctggacgta ccgcttcgag gcctacgtgc cgccgggcca gcgccggctc
     1441 ggctactacg cgctgccgat cctgcacggc gagcacgtcg tcggctggtg caacgcgtcc
     1501 gttcgcgaag gccggctgcg tctcgacagc ggattcgccg acggtcgtcg gcgcgacacc
     1561 gctttccgga cagaactggc agccgagcat gagcgactgg gcgagtttct cggggtcgcc
     1621 gagtcgctga gtcgctgagc gctaagtcga tgagtcgatg agccgatgag tggccgagtc
     1681 gccaagtgac cgagtcgctg actcgccaac tcgctgactc gccgagcgat cgaagcggcg
     1741 cgacgtttcc cgtgccgcat ccgccgactt ctccacttcc gcgacatgac gccgtcgtga
     1801 tccggcatcg cgacgcggac cggccgctcc gcgacaggca gcgaatggag gcgaacgact
     1861 cgtttggtag gctacgtcga tgatcttcaa ccgactgccg gcgccgttgc gcgcgtcgac
     1921 gcggcgcgtg tcgcaggcgc tcgggttgcc ctactggttc ccgcatgcgc cgctcgccct
     1981 gctggtcgcg ctcggcggct tccttctcct cgacggcgac ttcggccaga cgtggcaccg
     2041 ctacctcgac gaattcgcgc acggcagctt cagcctcggc ccgcgcctgc tgccgccgct
     2101 gctgctcggc gtcggcatgc tgacgatggc gctcggcctg ctcctgcgct cgcgtgtcgc
     2161 ctggacgatg gcgctgctgc tggtcggcac cgccgccgtc agcacccgct tcggcagcca
     2221 tgccggcggc ttcgcgctgc tcggctactt cgtggccctg ctcgcggcgc tgctgatcgc
     2281 ctggcgccat ttcgaccggt cgagcgtcgc cgccagcacc ctcttcgcgt tgacctcggt
     2341 ggcgatgctg ctgatgtacg ccaccttcgg aagctactac ctcggcacga gctacaagcc
     2401 gccgatcacc gacctcgtca ccgcgctgta ttacgcgatg gtgaccatga gcaccgtcgg
     2461 ctacggcgac atcgtgccgc agagcgccga ggccaagctg ttcacggtgt cgatcatcgt
     2521 cctcggcgtc gccgtgttcg ccacctcgct gacggcggtg atcgcgccga tggtcagccg
     2581 cagcttgctg cgaatcgtcg atcgcaaagg caaaaccatg acacgtgaag atcacttcgt
     2641 cgtcgtcggc agcacgccgc tcgccgacaa cacctggcgc gagctggccc gccgcggctg
     2701 cccggtgacc cgcatcctgc gcgaggcgcc cggcggcgac gccgacgacg agaccgacac
     2761 cgtcatcggc gatgcgagcc gcaccgacgt gctgaaacag gccggtgcgg accgtgcaac
     2821 cgcggtgctg gcgatgatgg ccgacgattc cgaaaacgcc ttcatcgttc tcgccgtgaa
     2881 ggagctcggc ggcggcgcgc agaccatcgc cgccgtcaac gattcgcggc acctgagccg
     2941 catcaagctg gtccagcccg atgtcgtgat cgcgccgcag gtcctcggcg ccgagctggc
     3001 ggcgatgctg ctctcgggcg aaaccgtgac gccggaattc gtgatgcagc gggtcttcca
     3061 gcgcgaggtg cccgccgcgg cgacgtcccc gggcagcgcg ccgcccgcgt cgggcaagcc
     3121 gcgcgagtaa ggcgaggagt cgcaggcgcg cgaaacgtgc tgcgctcgcc agggcgctct
     3181 tcggcgccgg ataatcgacc gttgctgcag gacatcgaga agcgatgcgt gtcgacggga
     3241 cggtacgcga ttgggcgcaa cgcctcgatc cggggtg
//
LOCUS       DASRWS010000017         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_7691, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000017 DASRWS010000000
VERSION     DASRWS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7691"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..291)
                     /gene="fliQ"
                     /locus_tag="VFF72_00275"
     CDS             complement(22..291)
                     /gene="fliQ"
                     /locus_tag="VFF72_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HZV91615.1"
                     /translation="MDSQQVFTFGQQSLWMLLMVSAPLLLTVLAVGLLVSIFQAATQI
                     HEATLSFVPKIIAAVAVLVIAGPWMLTSLVEFIQRTLQSIPSVVG"
     gene            complement(393..1097)
                     /gene="fliP"
                     /locus_tag="VFF72_00280"
     CDS             complement(393..1097)
                     /gene="fliP"
                     /locus_tag="VFF72_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059128.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZV91616.1"
                     /translation="MPAGAQGTAGNGATLPLLIGQGATGTSYSVPVQTLLFFTALSFL
                     PAVLLLMTSFTRIVIVLSLLRQALGTQAAPPNQVVIGLSLFLTFFVMGPTIDKVYKDA
                     YQPYADNQIAFEEAIKRGESPVRDFMMKQTRQADVMLFARLAKIDPSVKTADVPFKVM
                     VPAFVTSELKSAFQIGFLVFLPFLIIDMIVASVLMSLGMMMLSPVLISLPFKLMLFVL
                     ADGWNLLLGSLASSFA"
     gene            complement(1237..1596)
                     /gene="fliO"
                     /locus_tag="VFF72_00285"
     CDS             complement(1237..1596)
                     /gene="fliO"
                     /locus_tag="VFF72_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZV91617.1"
                     /translation="MIANGITSFLWFLVILALIPASLWFLKRTPLGGGGGQGQLRSVA
                     ALPLSSSQRIVTVEVGTGDDRRWLVLGVTPTSITTLHTMAAQPVEALPSTPAAHPAFA
                     QILGRFRRGATPGDDGR"
     gene            complement(1660..2085)
                     /gene="fliN"
                     /locus_tag="VFF72_00290"
     CDS             complement(1660..2085)
                     /gene="fliN"
                     /locus_tag="VFF72_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZV91618.1"
                     /translation="MATDNTQGATDPAAADWAAVDARGETASEVHAAADQVSPASFAT
                     FAPTTGSTAGNDINMILDIPVQLTVELGRTRIPIKNILQLAQGSVVELEALAGEPMDV
                     LVNGYLIAQGEVVVVNEKFGIRLTDIVTPSERMRRLSRG"
     gene            complement(2075..>2680)
                     /locus_tag="VFF72_00295"
     CDS             complement(2075..>2680)
                     /locus_tag="VFF72_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZV91619.1"
                     /translation="AGKYPTRIEGRDFSATEQRVIRRLLDVVMTEYRKAWAGIYPLEL
                     EFQRSEMQPQFANVATPGEIVVSASFTLEIGESSGTIHICIPYATFEPIRDVLYGQMQ
                     GDANAPDRRWVNLMTQQIQAAEVELVAELAQAPATVEQLLALKSGDFIELDLKPAIQA
                     KVAGVPVLDCHYGTSNGKYALKIDQLLSASTGGWLGETHGH"
BASE COUNT          450 a          860 c          966 g          404 t
ORIGIN      
        1 ccggagcgcc aacctcgcgc ttcagccgac gacggacgga atcgactgca gcgtgcgctg
       61 gatgaattcg accagcgacg tcagcatcca cgggccggcg atgacgagca cggcgacggc
      121 ggcgatgatc ttcggcacga acgacagggt cgcctcgtgg atctgcgtcg ccgcctggaa
      181 gatgctcacg agcaggccga cggcgagaac cgtcagcagc agcggcgccg agaccatcag
      241 cagcatccac aggctctgct ggccgaaggt gaagacctgt tgcgaatcca ttttttatgc
      301 gccgtagtga tgcgcggacc gggcgccggg ccgcggcagg cccccgcgcg cccgttccgt
      361 gggcggccgt gttcgagagc gtcggggcat cctcacgcga agctcgatgc gagcgagccg
      421 agcagcaggt tccagccgtc ggccagcacg aacagcatca gcttgaaggg cagcgagatc
      481 agcaccggcg acagcatcat catgccgagc gacatcagca cgctggcgac gatcatgtcg
      541 atgatgagaa acggcaggaa gacgagaaag ccgatctgga aggcgctctt cagctcgctg
      601 gtgacgaagg ccgggaccat caccttgaac ggcacgtcgg cggtcttcac cgacggatcg
      661 atcttggcca gccgcgcgaa cagcatcacg tcggcctggc gggtctgctt catcatgaag
      721 tcgcgcaccg ggctttcgcc gcgcttgatc gcctcctcga aggcgatctg gttgtcggcg
      781 tagggctggt aggcgtcctt gtagaccttg tcgatcgtcg gccccatgac gaagaaggtg
      841 agaaacaggc tcagtccgat caccacctgg ttgggtggcg cggcctgcgt gccgagcgcc
      901 tggcgcagca ggctgaggac gatgacgatg cgcgtgaagc tcgtcatcag cagcaagacc
      961 gcgggcagga agctgagcgc ggtgaagaag agaagggtct gcaccggcac cgagtagctg
     1021 gtgcccgtcg cgccctggcc gatcagcagg ggcagggtgg cgccgttgcc ggccgtgccc
     1081 tgggcgccgg ccggcatggc gagcaggccg agggtgaagg cgacgacgaa ggcgaagccg
     1141 atccaggcga gccggctgac ccgcgaccag gcgcgccgcg cgtcgccggc cgagtcgtcg
     1201 cgcaactcgc cgaacgacgt cgactggagc tggctttcag cggccatcgt cgcccggcgt
     1261 ggcgccgcgc cggaagcggc cgaggatctg cgcgaaggcc ggatgggccg ccggcgtgga
     1321 cggcagcgcc tcgaccggtt gcgccgccat ggtgtgcagc gtcgtgatcg acgtcggcgt
     1381 cacgcccagc accagccagc gccggtcgtc gcccgtgccg acttcgacgg tgacgatgcg
     1441 ctgcgagctc gacagcggca gtgccgcgac gctgcgcaac tgcccctggc caccgccgcc
     1501 gccgagcggc gtgcgcttca ggaaccacag gctcgccggg atcagcgcca ggatgacgag
     1561 aaaccagagg aacgaggtga tgccgttggc gatcatcggg ccggccgtgg gtgcgggaag
     1621 gaaagaggaa agcgacagaa gctcgcgcgc aagtgcgggc tagccgcggc tcaggcggcg
     1681 catccgttcg ctcggcgtga cgatgtcggt cagccggatg ccgaacttct cgttgacgac
     1741 gacgacctcg ccctgcgcga tgaggtagcc gttgacgaga acgtccatcg gctcgcccgc
     1801 cagcgcctcg agctcgacga ccgagccctg cgccagctgc aggatgttct tgatcgggat
     1861 ccgcgtccgg ccgagctcga cggtgagctg caccgggatg tcgaggatca tgttgatgtc
     1921 gttgccggcg gtcgagccgg tcgtcggcgc gaacgtcgcg aacgacgccg gcgacacctg
     1981 gtcggcggcg gcgtgcacct ccgaggcggt ctcgccgcgg gcgtcgacgg cggcccagtc
     2041 ggcggcggcc ggatcggtgg cgccttgggt gttgtcagtg gccatgcgtt tctccgagcc
     2101 agccgccggt ggaggcggac agcagttgat cgatcttcag cgcgtacttg ccgttcgagg
     2161 tgccgtagtg gcaatcgagc accggcaccc cggcgacctt ggcctggatg gccggcttca
     2221 ggtcgagctc gatgaaatcg cccgacttca gcgccagcaa ttgctcgacg gtggcgggcg
     2281 cctgcgccag ctcggcgacg agctcgacct cggcggcctg gatctgctgc gtcatcaggt
     2341 tgacccagcg ccggtcgggc gcgttggcat cgccctgcat ctggccgtag agcacgtcgc
     2401 ggatcggctc gaaggtcgca tagggaatgc agatgtggat cgtgccgctg ctctcgccga
     2461 tctcgagcgt gaacgatgcc gagacgacga tctcgccggg tgtcgcgacg ttggcgaact
     2521 gcggctgcat ctccgagcgc tggaactcga gctcgagcgg gtagatgccc gcccaggcct
     2581 tcctgtattc cgtcatcacg acgtcgagga ggcggcggat gacgcgctgc tcggtcgccg
     2641 agaagtcgcg cccctcgatg cgcgtcggat acttgccggc
//
LOCUS       DASRWS010000018         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3748094, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000018 DASRWS010000000
VERSION     DASRWS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3748094"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFF72_00300"
     CDS             <1..240
                     /locus_tag="VFF72_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91620.1"
                     /translation="WGRDGWMGIEDTPTIDSELGFHVAVLATAGLAPGSWINFTWRDL
                     ETGEWSGCDERVGVMAAEQPTASFTTLQPGARAAK"
     gene            261..1949
                     /locus_tag="VFF72_00305"
     CDS             261..1949
                     /locus_tag="VFF72_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV91621.1"
                     /translation="MPQPSYVHGASATPLIGETIGVHFDRIAGRFAERDALIVRHQQV
                     RWTYRELQRRVDAFAAGLLAFGLEPGERVGIWSPNNAEWVVAQFATAKAGLILVNINP
                     AYRLAELEYALNKAGCSALITAERFKASDYVGMLNQLAPELADAAPGKLEAARLPCLR
                     LAINIGGDSVPGMLRFDEIYDLGGDVERERIAALADTLQFDDPINIQFTSGTTGFPKG
                     ATLTHHGILNNGFFLGEAMRFTEHDRVCIPVPLYHCFGMVIGNLGCLTHGAAMVYPSE
                     GFEPLATLETIEAERCTALYGVPTMFIAQLDHPEFPRFALASLRTGIMAGSPCPIEVM
                     RRAVERMNLREIVIGYGMTETSPASTVTTTDDPIERRVATVGRAMPHTEVKIVDAEGR
                     IVPRGTTGELLTRGYLVMLGYWDDEEKTREAIDPAGWMHTGDLAVIDEEGYCNIVGRI
                     KDMIIRGGENIYPREIEEFLYRHPKVQDVQVFGIPDPRYGEELCAWIRLRNGDEAMAE
                     EIRAFCQGQIAHFKIPRHIKFVDDFPMTVTGKIQKFAMREAMIRELGLTLAKTA"
BASE COUNT          410 a          702 c          727 g          377 t
ORIGIN      
        1 tggggccggg atggctggat ggggatcgag gacacgccga cgatcgatag cgagctcggt
       61 ttccatgtcg cggtgttggc gaccgcgggg ctggcgccgg gcagctggat caatttcacc
      121 tggcgcgatc tggaaaccgg cgaatggtcg ggctgcgacg aacgggtcgg cgtgatggcg
      181 gccgaacaac ccacggcgag cttcaccacg ctgcagccgg gtgcgcgggc ggcgaaataa
      241 ttccggggag agatcgacga atgccgcagc cgagctatgt tcatggcgcg agcgccacgc
      301 cgctaatcgg cgagacgatc ggcgtccatt tcgaccgcat cgccggccgt ttcgccgagc
      361 gcgatgcgct gatcgtccgg caccagcagg tccggtggac gtaccgcgag cttcagaggc
      421 gcgtcgatgc gttcgcggcg gggctgttgg cgttcggtct cgaacccggc gagcgggtcg
      481 ggatatggtc gccgaacaac gccgaatggg tcgtcgcgca gttcgccacc gccaaagccg
      541 ggctcatcct cgtcaacatc aacccggcct accggctcgc cgagctcgaa tatgcgctga
      601 acaaggcggg ctgctcggcg ctgatcaccg cggagcggtt caaggccagc gactatgtcg
      661 ggatgctgaa ccaactcgcg cccgagctgg ccgacgccgc tccgggtaaa ctcgaggccg
      721 cccgactgcc ctgcctgcgg ctcgcgatca acatcggcgg cgacagcgtc cccggcatgc
      781 tgcgcttcga cgagatttac gatctgggcg gcgatgtcga acgggagcgc atcgccgcgc
      841 tcgccgatac gctgcaattc gacgacccga tcaacattca gttcacgtcg ggcacgaccg
      901 gcttcccaaa gggcgcgacg ctgacccatc acggcatcct caacaacggc ttctttctcg
      961 gcgaagcgat gaggttcacc gagcatgatc gcgtctgcat tccggtgccg ctctaccact
     1021 gttttggcat ggtgatcggc aatcttggct gcctgacgca tggcgcggcg atggtctatc
     1081 cgagcgaggg gttcgagccg ctcgcgacgc tcgaaacgat cgaggcagag cgttgcaccg
     1141 ctctctacgg cgtgccgacg atgttcatcg ctcagctcga tcatcccgag ttcccgcgct
     1201 ttgccctcgc ctcgttgcgc accgggatca tggcggggag cccctgcccg atcgaagtca
     1261 tgcggcgtgc cgtcgagcgc atgaatctgc gcgagatcgt catcggctac ggcatgaccg
     1321 agaccagccc cgcgagcacc gtcacgacga ccgacgatcc gatcgagcgg cgtgtggcga
     1381 cggttggccg cgcgatgccg cacaccgagg tcaagatcgt cgatgcggag ggccgcatcg
     1441 tgccgcgcgg cacgaccggc gagttgttga cccgcggcta tctcgtcatg ctcggctatt
     1501 gggacgacga ggagaagacg cgcgaggcga tcgatcccgc cggctggatg catacgggcg
     1561 acctcgcggt catagacgag gagggctatt gcaacatcgt cggccgcatc aaggacatga
     1621 tcatccgcgg cggcgagaac atctatccgc gcgagatcga ggaatttctt taccgccatc
     1681 cgaaagttca ggacgtgcag gtgttcggga tccccgatcc gcgctacggc gaggaattat
     1741 gcgcctggat ccgcttgcgt aatggcgacg aggcgatggc ggaggagatc cgcgccttct
     1801 gccaaggcca gatcgcgcat ttcaagatcc cgcgccatat caaattcgtc gacgatttcc
     1861 cgatgacggt caccggaaag atacagaaat tcgccatgcg cgaagcgatg atccgcgagc
     1921 tgggcctcac gctcgcaaag accgcgtgag tgcccttcgc cgcaccaacg cgaaccgcag
     1981 gtcctgcccc gaagcggccc ctcggctcga tactgcggag cgatccgcga gatcgcgagc
     2041 tgcggttgga tcgctaccga agaagttgct cgaggaggct ccgagaggct gcttcagtga
     2101 aactgcccga ttccccgttt gtcaggacga ccacgccggc cttagcggtc ggtgctagaa
     2161 cgatcgcagc cctgaagccg ggatttgcac cattgtgata aacaaggtgc gcaccg
//
LOCUS       DASRWS010000019         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_586556, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000019 DASRWS010000000
VERSION     DASRWS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586556"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFF72_00310"
     CDS             complement(<1..203)
                     /locus_tag="VFF72_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91622.1"
                     /translation="MSSKATSRAASAVADKQRKIRRQLARHDPLEARSSSSRGAIRVG
                     AVAPGPVWHSSIRRFAARQGEEFG"
     gene            complement(311..649)
                     /locus_tag="VFF72_00315"
     CDS             complement(311..649)
                     /locus_tag="VFF72_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91623.1"
                     /translation="MFLTEQHRYSDALDAFTDGIAKNPGNSMNYYGFGQAAQLGKERV
                     GQGIENLLKFLTLPHDWLPGTPTYKQAHYQLGRLYALSGDRAGEKAQYVAALDLDPTY
                     EPARSALARM"
     gene            complement(704..1357)
                     /locus_tag="VFF72_00320"
     CDS             complement(704..1357)
                     /locus_tag="VFF72_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV91624.1"
                     /translation="MPFAGYGTHGGACLTSAICAGASAVIKRGIVSMMVSAAALFSLG
                     AKAGVPEDPGMRMVAKENYGGARAYFQGLLKGDPRDAAAAAGMATLELTRGNNESSVE
                     WARKAIELAPTNAAYRMLLGVAYGQYVRDVNPLSQLGIAYKVRDSFEQAVQLAPDSAE
                     AHAGLAKYYILAPGIAGGSTRKAEEQLAALDRLDAAQAAAVRATLAQQHKDLAKTED"
     gene            complement(1449..2063)
                     /locus_tag="VFF72_00325"
     CDS             complement(1449..2063)
                     /locus_tag="VFF72_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZV91625.1"
                     /translation="MRGLRRAATTCCAALWLCACGMLPQPPASPALHDFGLPVGAPAV
                     TLAGGQVAVAPVSSPSWLANSRIRYRLLFDEPARLRAYAQNEWIAPPAELMTARLRLA
                     FGNPDFAVAGASGEPAYRLEVELLDFEQVFDTAHSARSNLRAVVSLRSTKDGRVLGPR
                     LFDVSMASSPSVRGAIDSGGAAVERLVAQIVLWADGFRPAAMRP"
     gene            complement(2060..2953)
                     /locus_tag="VFF72_00330"
     CDS             complement(2060..2953)
                     /locus_tag="VFF72_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZV91626.1"
                     /translation="MNNKAYAFVTGLFVLLLAAGVIIAAVWLSGSLQKTKPYVVVTTE
                     DVNGLAPQSNVTFRGIVAGKVTALRLDPDDPHRTRIDIAVASTTPVTRDTYAVLKMQL
                     TGLSQLALQTTGTSNALLATSAKAPARIAMRPSLLDRLSQSASQVMAQLDTLATSLNA
                     AVDPENRAHLAALLAHADLASARLARLTSDLDVAARRLPGLSSRMESTLQRLDALSVE
                     LDKVAASAGKLSATGVAAGEKIDRVTLPRLDSALARLGASADEIRRLSRSLHDDPQQF
                     LMGPPRPPPGPGERGYRGPSR"
BASE COUNT          499 a         1034 c         1035 g          494 t
ORIGIN      
        1 ccgaactctt caccttgtct ggcggcgaat cggcggattg aggagtgcca gacggggccc
       61 ggcgccaccg cgccgacgcg gatcgcaccg cgtgacgaag acgaccttgc ctcgagcggg
      121 tcatggcgag ccagctggcg ccggatcttg cgctgcttgt cggcgaccgc gctcgcggca
      181 cgcgaagtcg ccttgctgct cattgcttct tcccatcggt catcgctttc gcgaaacggc
      241 aaatcacttg ccgccgccaa ccctggtcgg cattccagcg aggccgctgg ccgacggccg
      301 gccctgtttg ctacatgcgc gccagcgccg atctggctgg ctcgtaggtc ggatcgagat
      361 cgagtgcggc cacgtattga gccttctcgc cagccctgtc tccggacagg gcgtaaagcc
      421 tgccgagctg gtaatgagcc tgcttgtagg tcggcgtgcc tggcaaccaa tcgtgcggca
      481 atgtcaggaa cttgagcagg ttctcgatgc cctgcccgac ccgctccttg cccagttgcg
      541 ccgcctggcc gaacccgtag tagttcatgc tgttgccagg attcttggcg atgccgtcgg
      601 tgaatgcgtc gagcgcgtcg gagtagcgat gctgctcggt cagaaacacc cccagccagt
      661 actgcccttc gccgagcttg tcgagcctgg cgctggcgcg cagttagtct tcggtcttag
      721 cgaggtcctt gtgttgctgc gccagggtgg cgcgaacggc agcggcctgg gcggcgtcga
      781 gccggtcgag ggcggcaagc tgttcctcgg cctttcgcgt gctgccgccc gcgatccctg
      841 gcgcaaggat gtagtacttg gcgagaccgg cgtgcgcctc cgcactgtcc ggggccaact
      901 gcacggcttg ctcgaacgaa tcccgaacct tgtaggcgat gccgagctgg ctcaggggat
      961 tgacgtcgcg aacatattgg ccgtaagcaa cgcccaggag catgcgatat gcggcattcg
     1021 tcggcgccag ctcgatggcc ttgcgcgccc attcgacgct ggactcgttg ttgccgcgcg
     1081 tcagctccag cgtcgccatg ccggcggccg ccgcagcatc acgcggatcg cctttcagaa
     1141 gaccctggaa ataggcccgc gcaccgccat agttttcctt cgccaccatc cgcatgccgg
     1201 ggtcctccgg cacgccggcc ttggccccga gagaaaagag tgccgccgcc gataccatca
     1261 tcgaaacgat cccacgcttg atcaccgcgc tcgctccagc acaaattgca ctcgtcagac
     1321 aagcgccgcc atgcgtgccg taaccggcga acggcacggt gtcttgcctg cgaccgaacc
     1381 ggtcggacag gcggcagcgg cgcaagttcc gatcggtccg cgcccgatgc gcggcgcgtc
     1441 ctgctgcgtc acggccgcat cgcggccggc cggaacccgt cggcccagag aacgatttgc
     1501 gccacgagtc gttcgacggc cgcgcccccg gagtcgatgg cgccacgcac gctcggcgac
     1561 gaagccatgg agacatcgaa gagccgcgga cccagcaccc gtccatcctt cgtggaccgc
     1621 aggctgacga cggcgcgcag attcgacctc gcggaatgcg ccgtgtcgaa cacctgttcg
     1681 aagtcgagaa gctccacttc caggcggtag gccggttcac cggacgcgcc ggcgacggcg
     1741 aagtccgggt tgccgaaggc cagtcgcaag cgtgccgtca tcagctccgc aggcggggcg
     1801 atccattcgt tctgggcgta ggcgcgcagt cgcgccggct cgtcgaagag cagtcgatag
     1861 cgaatccgcg aattggcgag ccacgagggc gagctcacgg gcgcgaccgc gacctgtccg
     1921 cccgcaaggg tcaccgccgg cgcaccgacc ggcagaccga agtcgtggag cgccggcgac
     1981 gcaggcggct gcggcaacat cccgcaggcg cacagccaca gcgctgcgca gcacgtcgtc
     2041 gctgcccgtc gcaggcccct catcgcgaag gcccgcggta gccacgctcg cccgggcccg
     2101 gaggcggcct cggcggcccc atcaggaact gctgcgggtc gtcgtgcagg cttcgcgaca
     2161 gccgacggat ctcgtccgcc gaggcgccga gccgggccag cgccgaatcg agccgcggca
     2221 acgtgacgcg gtcgattttc tcgccggcgg ccacacccgt ggcgctcaat ttgccggcgc
     2281 tcgccgcgac cttgtcgagc tcgaccgaca gcgcgtcgag cctttgcaag gtggattcca
     2341 tcctcgacga caagccaggc agccggcgtg cggcgacatc gaggtcggat gtcaaacgcg
     2401 ccaatcgcgc gctggccagg tcggcgtgcg cgagcagtgc cgcgagatgg gcacgatttt
     2461 cgggatcgac ggcggcgttg agcgacgtcg ccagggtgtc gagttgcgcc atgacctggc
     2521 ttgccgactg gctcaatcga tcgagcagcg aaggccgcat ggcaatccgg gccggcgcct
     2581 tggccgaggt cgcgagaagg gcgttgctgg tgccggtggt ctgcaacgcg agctgcgaca
     2641 ggcccgtcag ctgcatcttg agcaccgcgt aggtatcgcg ggtgaccggt gtggtcgagg
     2701 caacggcgat gtcgatccgg gtgcggtgcg gatcgtccgg gtccagccgc aaggcggtga
     2761 ccttgcccgc gacgatgccg cgaaacgtca cgttcgattg cggcgccagg ccgttcacgt
     2821 cttccgtcgt gacgacgacg tagggcttgg tcttctgcaa cgagccgctg agccagacgg
     2881 cagcgatgat gacgcccgcc gccaggagca ggacgaacag ccccgtgacg aatgcgtagg
     2941 ccttgttatt catgagcgga ttgcgccatg tcggcgaagg cgcgtcgccc gcgaatgcca
     3001 tggaagaagt cgcgcacgaa aggatgaccc gactccagca cggcgtcgag gctgccgagg
     3061 ac
//
LOCUS       DASRWS010000020         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2888852, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000020 DASRWS010000000
VERSION     DASRWS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2888852"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFF72_00335"
     CDS             <1..972
                     /locus_tag="VFF72_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV91627.1"
                     /translation="RPVIEVDYDPSQAVVLATVRGLLAQHVMDAVSRDVFNSTGPVGA
                     SDGLAGMRRQLDASSGPPDDARRHLATMFESIERVQADRATDPATSAAASQPAAATAG
                     AFTLPFETNLREATAAEGNRPYNSYAHSFAGMGVQFILFMGIEVGVGVLLARRLGLWK
                     RLRAAPLSRSVLLGSHILSGATIALVLLAIIYAAAMTIFRVRIEGSVVGFAGVAVAFA
                     LLTSSFGLLIAAIGKTPEATRGLAIFATLVMVMLGGAWVPSFVFPPWLQTASLAVPTR
                     WAVDGLDAMTWRGLGAEAALAPIAVLLGFSALFAAIAIWRFDWEEPR"
     gene            complement(969..>2005)
                     /locus_tag="VFF72_00340"
     CDS             complement(969..>2005)
                     /locus_tag="VFF72_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZV91628.1"
                     /translation="EEKSALAQLRRRQLDAARLAVAGNTVMQALRIASLHAQIDTVES
                     ILDEDRQNLKLVHLAFDAGSVSRLDIVSAESQLASDTTSLPPLRQELSVAEHALAVVV
                     GSPPASAALPSFDLAGLTLPRRLPLSLPSELARRRPDILAAEAQLHAATARVGIADAN
                     LYPHITLSAAGSGQSATLAHLFDPSSSAWSLAAGIVGPLFDGGTLHAEQRAAVAAMRA
                     SAANYEQTVLTAFGQVADTLLALEHGSEQLRAQRQAVRAAGDSLALTRKSYHEGNVGV
                     LQVLDAERLYQQARLGVVRAEAQRFADTAQLFLALGGAGPDDDELASTIAAGPSGAKR
                     QARPATPARP"
BASE COUNT          257 a          715 c          733 g          300 t
ORIGIN      
        1 cggccggtca tcgaagtcga ctacgacccg tcgcaggccg tcgtcctggc gacggtccgc
       61 ggcctgctcg cgcagcacgt gatggacgcc gtgagccgcg acgtgttcaa cagcaccggg
      121 ccggtcggcg ccagcgacgg cctcgccggg atgcgacgac agctcgatgc gtcgagcggg
      181 ccgccggacg atgcccgacg ccaccttgcg acgatgttcg aaagcatcga gcgtgtccag
      241 gccgatcgcg cgaccgaccc ggccacgagc gccgcggcgt cgcaaccggc ggcggccacg
      301 gcgggcgcct tcacgctgcc gttcgagacg aaccttcgcg aagcgactgc cgccgaaggc
      361 aaccgcccgt acaacagcta cgcccactcg ttcgccggca tgggcgtgca gttcatcctg
      421 ttcatgggca tcgaggtcgg cgtcggcgtt ctcttggccc gacgcctcgg cctgtggaag
      481 cgcttgcgcg ccgcaccgtt gtcgcgctcc gtcctgctcg gcagccacat cctcagcggc
      541 gcgaccatcg cgctggtgct cctggcgatc atctacgcgg cggcgatgac gatctttcgc
      601 gtccgcatcg agggcagcgt cgtcggcttc gcgggagtcg ccgtcgcgtt cgcgctgctc
      661 acctcgagct tcgggctgct catcgccgcg atcggcaaga cgcccgaagc gacgcgcggg
      721 ctggccatct tcgccacgct ggtgatggtg atgctcggcg gcgcctgggt gccgtcgttc
      781 gtctttccgc cctggctgca gaccgcgtcg ctggcggtgc cgacgcgctg ggcggtcgac
      841 ggactcgacg ccatgacctg gcgcggcctc ggcgccgaag ccgcgctcgc gccgatcgcc
      901 gtcctgctcg gattcagcgc gctgttcgcg gcgatcgcga tctggcgctt cgactgggaa
      961 gagccgcgct agggccgcgc cggcgtcgcc ggccgcgcct gtcgcttcgc tcccgacgga
     1021 ccggcggcga tggtcgaggc gagctcatcg tcgtccgggc cggcgccgcc gagggcgagg
     1081 aaaagctgtg ccgtgtcggc gaagcgctgc gcttcggcgc gaaccacgcc caggcgcgct
     1141 tgctggtaga ggcgctcggc gtcgagcacc tgcagcacgc cgacgttgcc ctcgtgatag
     1201 ctcttgcgcg tgagggccag cgagtcgccg gcggctcgca cggcctggcg ctgggcgcga
     1261 agctgctcgc tgccgtgctc gagcgcgagc agcgtatcgg cgacctggcc gaaggccgtc
     1321 agcaccgtct gctcgtagtt cgccgcgctg gcgcgcatcg ccgcgacggc ggcacgctgc
     1381 tcggcatgca gcgtgccgcc gtcgaagagc gggccgacga tgccggccgc gagactccag
     1441 gcgctgctcg acggatcgaa aaggtgcgca agcgtcgcgc tttgtccgct gccggccgcg
     1501 ctgagcgtga tgtgcggata gaggttcgca tcggcgatgc cgacccgcgc cgtcgccgcg
     1561 tgcagctgcg cctcggccgc gaggatgtcc ggacggcgcc gcgccagctc cgacggcagg
     1621 ctgagcggca gccggcgcgg cagcgtcagg cccgcgagat cgaacgacgg cagcgccgca
     1681 ctcgcaggcg ggctgccgac gacgacggcc agcgcgtgct cggccacgct cagctcctgg
     1741 cgcagcggcg gcagcgacgt cgtgtcgctg gccagctggc tctcggcgct gacgatgtcg
     1801 agccgcgaca ccgagccggc gtcgaaggcg aggtggacga gcttcaggtt ctgtcgatcc
     1861 tcgtcgagga tggattcgac ggtgtcgatc tgcgcgtgca gcgaggcgat gcgcagcgcc
     1921 tgcatgacgg tgttgccggc cacggccagg cgggccgcat cgagctgtcg gcgccgcagc
     1981 tgcgcgagcg ccgatttctc ctcga
//
LOCUS       DASRWS010000021         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3461583, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000021 DASRWS010000000
VERSION     DASRWS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461583"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1183
                     /locus_tag="VFF72_00345"
     CDS             38..1183
                     /locus_tag="VFF72_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HZV91629.1"
                     /translation="MNLRHVSRPGEGVDAIETPALVIDVDAMQRNLVAMAAFAAGHRL
                     RLRPHAKMHKCAAIARLQVEAGAVGVCVQKTSEAEALAAAGTCDIYVSNEVVDRRKLA
                     RIAALAAHIRVAIAVDSAEGVDRLAEAMRAQPGAGIDVFVEIDVGQGRCGVAAAAAGA
                     LAHRVVSHAPPDGGLRFAGLQAYHGGAQHRRAAVEREAASLHAAALVRAAQASITASG
                     IACPLVTGGGTGTFAFDAAHGVWGELQCGSYLFMDRDYADNAATPGAPRFEQALFVKS
                     QVMSRGAAHVVVDAGHKAHAVDSGPPRVWRHDLDVKSVSDEHTVLQARPASGAASLPG
                     IGDTVWLIPGHCDPTVNLHSSYVVVRGGLDAGRVEAIWPIEARGCLT"
     gene            1390..>2137
                     /locus_tag="VFF72_00350"
     CDS             1390..>2137
                     /locus_tag="VFF72_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZV91630.1"
                     /translation="MHVPSPRRPHAAAAPLALAAQPFAESKTLVPAVSRALTLLERLA
                     DAREPMSLARLASDLGLPKSSVHGLCSTLVSFGYLRRQGDGAFLIGPRVMGLAEAFVS
                     GTDVAQEFAALWADRRSAPEETVVLSVLSGSEALYVAVRNSARPLGLAFTVGMRLPAH
                     LSGSGKAMLAHLPDDEVTRRLAGNIGVRLTHKGPRSLDALLKELALTRRRGYSIDDEA
                     VREGVYSFGAPVFDVTGHVAAAVSVCIHKAL"
BASE COUNT          286 a          784 c          750 g          317 t
ORIGIN      
        1 tcggtgcagc gtccgtgggg cagcccgagg cgcggcgatg aaccttcgcc acgtgtcacg
       61 cccgggcgag ggcgtcgatg cgatcgagac gccggcgctg gtgatcgacg tcgacgcgat
      121 gcagcgcaat ctcgtcgcga tggccgcgtt cgcggcgggc cacagactgc gcctgcggcc
      181 gcacgcgaag atgcacaagt gcgcggcgat cgccaggctg caggtcgagg ccggcgccgt
      241 cggcgtctgc gtgcagaaga cgagcgaagc ggaagcgctc gccgccgccg gcacctgcga
      301 catctatgtc tccaacgagg tcgtcgatcg ccgcaagctg gcgcgcatcg cggcgctggc
      361 cgcgcacatt cgcgtcgcga tcgcggtcga cagcgccgaa ggcgtcgatc gcctcgccga
      421 ggcgatgcgc gcgcaacccg gcgccggcat cgacgtcttc gtcgagatcg atgtcggcca
      481 gggccgctgc ggcgtcgccg cggccgcggc cggcgcgctc gcgcatcgcg tcgtctcgca
      541 tgcgccaccc gacggcggcc tgcgcttcgc gggcctgcag gcgtatcacg gcggcgccca
      601 gcaccgacgc gcggcggtcg agcgcgaagc ggcgtcgctg catgctgcgg cgctggtccg
      661 cgccgcccag gcgagcatca ccgcgtcggg aatcgcctgc ccgctcgtca ccggcggtgg
      721 caccggcact ttcgccttcg acgccgccca cggcgtgtgg ggcgaactgc agtgtggcag
      781 ctacctcttc atggaccgcg actacgccga caacgccgcg acgcccgggg cgccgcgttt
      841 cgagcaggcg ctcttcgtga agagccaggt catgagccgc ggcgctgcgc atgtcgtcgt
      901 cgatgcgggg cacaaggcac acgccgtcga ttcggggccg ccgcgggtct ggcggcacga
      961 cctcgacgtc aagtcggtga gcgacgagca caccgtgctg caggcgcggc cggcaagcgg
     1021 cgccgcgtcg ctgcccggga tcggcgacac cgtctggctg attcccgggc actgcgaccc
     1081 gaccgtcaac ctgcattcgt catacgtcgt cgtgcgcgga ggtctcgacg ccggtcgcgt
     1141 cgaggcgatc tggccgatcg aggcccgggg ctgcctgacc tgatctgaat cccgcgactg
     1201 tcggcgccac gccaggcgat ctcaggcgct gcgcgttttg cgcagttcgc gggcaaacgc
     1261 tcagaggaca aaccccgatt cgagggcgcc cgccggcttt cgccgcttcg cgcctgtcta
     1321 gactcgcgat cggtccgctg aagcgcattc agccgaatga acaggcggcc cccacctcgc
     1381 ccttctccaa tgcacgttcc gtcgccgcgc cggccgcacg ccgccgccgc tccgctcgcg
     1441 cttgccgcgc agccgttcgc cgaaagcaag acgctggtgc ccgccgtgtc gcgggcgctg
     1501 acgctgctcg agcggctggc cgacgcgcgc gaaccgatga gcctggcccg cttggccagc
     1561 gatcttggcc tgcccaagag cagcgtccac ggcctgtgca gcacgctggt ctcgttcggc
     1621 tacctgcgcc gccagggcga cggcgccttt ctcatcggtc cgcgcgtcat gggtctggcc
     1681 gaggcgttcg tgtcgggcac cgacgtcgcc caggagttcg cggcactgtg ggccgatcgc
     1741 cgcagcgccc ccgaggagac cgtcgtccta tcggtgcttt caggcagcga ggcgctctac
     1801 gtcgccgtgc gcaacagcgc tcgtccgctc ggccttgcct tcaccgtcgg catgcggttg
     1861 ccggcccacc tctccggcag cggcaaggcg atgcttgcgc accttccgga cgacgaggtg
     1921 acccgccgtc tcgccggcaa catcggcgtg cgcctgactc acaaggggcc gcgcagcctc
     1981 gacgcgctgc tcaaggagct ggcgctgacg cgacgacgcg gctacagcat cgacgacgag
     2041 gcggtgcgcg aaggcgtcta ttcgttcggc gctccggtgt tcgacgtcac ggggcacgtc
     2101 gcggccgcgg tctccgtctg catccacaag gcgctgc
//
LOCUS       DASRWS010000022         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2312491, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000022 DASRWS010000000
VERSION     DASRWS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312491"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFF72_00355"
     CDS             <1..479
                     /locus_tag="VFF72_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HZV91631.1"
                     /translation="QLYTRAGNFTLDSAGNMVTPDGARVEDPTGAPLNLAALTNGTYV
                     SWSIDSSGNINGVDNTGATTVLGQVGIATFANPNGLQKVGDTAFAASANSGTPSIGTA
                     NSGSRGSISSGYVEMSNVDLSQELTNLIISERGFQANSKVITTSDEILQTLVNLKS"
     gene            582..854
                     /locus_tag="VFF72_00360"
     CDS             582..854
                     /locus_tag="VFF72_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HZV91632.1"
                     /translation="MLQLTRLSGPVFALNPDLIERAEATPDTVVTLVNGTRYVIRESL
                     DELTALIRNYRADIICAAQLPAEHRHPPRPKDPSRPTVVALRNGER"
     gene            854..1621
                     /locus_tag="VFF72_00365"
     CDS             854..1621
                     /locus_tag="VFF72_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HZV91633.1"
                     /translation="MDPSTIIGLGIALGAILVSMIMDGGNIGAMFLIPPMILVFGGTI
                     GAAMAGTLMSDAKAMPKSLVRAFTAKVTTAHDSVAIIVRMAERARRDGLLALEGDVSE
                     VEDPFLRRALEMAIDGTDADEVADILGAEVDAKRAGDGAGAKLFHDMGGYSPTIGIIG
                     TVLGLIHVLGSLTDPSSLGAKIASAFVATLWGVLSANIFWFPLANRLKRISEAECEQM
                     EVTIEGVLAIQAGSNPRLVAQKLQSMLPNVEPQKEVA"
     gene            1621..>2065
                     /locus_tag="VFF72_00370"
     CDS             1621..>2065
                     /locus_tag="VFF72_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZV91634.1"
                     /translation="MPARPSGRSSAKGSSQRSRRRRGGHGGESHEGSERWLVTYADML
                     TLLLVLFIVLFSISVVNTSKFIALKTSLSSAFGDGPKGILSGGNGLNENSAEQEGQQD
                     IMAGVPVAPDTGKQTVTDTNTGATPADLTAKVQAEVDKYKEIERAI"
BASE COUNT          371 a          717 c          677 g          300 t
ORIGIN      
        1 agcagctgta cacgcgagcc ggcaacttca cgctcgactc ggccggcaac atggtcacgc
       61 cggacggcgc gcgcgtggaa gacccgacgg gtgccccgct caacctcgcg gcactgacga
      121 acggcaccta cgtgtcctgg agcatcgact ccagcggcaa catcaacggg gtcgacaaca
      181 ccggtgccac cacggtcctc ggccaggtcg ggatcgccac cttcgccaac ccgaacggcc
      241 tgcagaaggt cggcgacacc gcgttcgccg cctcggccaa ctcggggacg ccgtcgatcg
      301 gtacggccaa ctcgggctcg cgcggcagca tcagctcggg ctacgtcgag atgagcaacg
      361 tcgacctgtc gcaggagctg accaacctga tcatctccga gcgcggcttc caggcgaact
      421 cgaaggtgat caccacgtcg gacgagatcc tgcagaccct cgtcaacctc aagagctgac
      481 tttcgcgctc gcgacgggcc ggctcaagac cggcccgtcg cggccgattg gtacttatcg
      541 aggggccacg gacggccccg aagaatccaa ggacggacat cgtgttgcag ctgaccagac
      601 ttagcggccc ggtcttcgcg ctcaaccccg atctcatcga gcgcgcggag gccacgccgg
      661 acaccgtcgt gactctcgtc aacggcacca gatacgtgat ccgcgaatcg ctcgacgagc
      721 tgaccgcgct gatccgcaac tatcgcgccg acatcatctg cgcggcccag cttcccgccg
      781 agcacaggca cccgccccgc ccgaaggatc cgtcccgccc gaccgtggtc gcgctgcgca
      841 acggagagcg ctgatggatc cgtcgacgat aatcggcctg ggcatcgcgc tcggcgccat
      901 cctggtctcg atgatcatgg acggcggcaa catcggcgcc atgttcctca tcccaccgat
      961 gatccttgtc ttcggtggca ccatcggcgc cgcgatggcg ggcacgctca tgagcgacgc
     1021 caaggcgatg ccgaagtcac tcgtccgcgc gttcaccgcc aaggtcacga ccgcgcatga
     1081 cagcgtcgcg atcatcgtca ggatggccga gcgcgcacgc cgcgacggcc tgctggccct
     1141 cgagggtgac gtgagcgagg tcgaggaccc gttcctgcgc cgcgccttgg agatggccat
     1201 cgacgggacc gacgcggacg aggtcgccga catcctcggc gccgaggtcg acgccaagcg
     1261 ggccggcgac ggagccggtg cgaaactgtt ccacgacatg ggcggctact cgccgacgat
     1321 cggcatcatc ggtaccgtcc tcggcctgat ccacgtcctc ggcagcctga ccgacccgtc
     1381 gtcactcggc gcgaagatcg cgagcgcgtt cgtcgcgacg ctgtggggcg tcctgtcggc
     1441 caacatcttc tggttccctc tcgcgaaccg cctcaagcgc atcagcgagg ccgagtgcga
     1501 gcagatggag gtgacgatcg agggggtgct ggcgatccag gccgggtcca acccgcgact
     1561 ggtggcccag aagctgcaga gcatgctgcc gaacgtcgag ccgcagaagg aggtggcctg
     1621 atgccggccc ggccgtccgg gcgctcgtcg gccaagggca gctcgcagcg ctcgaggcgg
     1681 cgacgcggcg gccacggcgg cgagagccac gagggttccg agcgctggct ggtcacctac
     1741 gccgacatgc tgacgttgct gctcgtgctc ttcatcgtgc tgttctcgat ctcggtcgtg
     1801 aacacgtcga agttcatcgc gctgaagacc tcgctgtcgt cggctttcgg ggacggcccg
     1861 aagggcatcc tcagtggtgg caacggtctc aacgagaact cggccgagca ggaggggcag
     1921 caggacatca tggccggcgt tcccgtcgcg ccggatacgg gcaagcagac cgtgaccgac
     1981 acgaacaccg gcgcgacccc ggccgacctc acggcgaagg tgcaggccga ggtcgacaag
     2041 tacaaggaga tcgagcgggc gatca
//
LOCUS       DASRWS010000023         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2599621, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000023 DASRWS010000000
VERSION     DASRWS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599621"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFF72_00375"
     CDS             complement(<1..617)
                     /locus_tag="VFF72_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZV91635.1"
                     /translation="MKPSTALALVAVVLLGSCASAPTLSPAPASARRAIDAIDTVVVI
                     YAENRSFDTFYGLFPGANGIPGLNPSAIGAIAPQRDVDGAVLPVLPPVWGGLTAAGQA
                     QVVTQAQTVGMPNQPFQIDGPNAIDGHGIAVPTTVTTRDLVHRFYNNIMQIDGGRNDR
                     FAAYSDAGALSMGHYDGRHMALWEVARRFTLADAFYMGAFGGSFL"
     gene            715..1974
                     /locus_tag="VFF72_00380"
     CDS             715..1974
                     /locus_tag="VFF72_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZV91636.1"
                     /translation="MPADLTAEAVPPSWPGPGTSRIPFAVYTSESLHRRELERFFYRG
                     HWCYVGLEAEVPRPGDFKRTVIGERSVILVRADDGSLNVVENVCAHRGMSFCRERHGN
                     RKDFTCPYHQWNYSLKGDLQGVPFRRGVRHEGIVQGGMPADFKPQEHGLTKLRVAARG
                     GVVFASFDAEVESLEDFLGPTILRYYDRLFNGRELRLLGYNRQRIPGNWKLMQENIKD
                     PYHPGLLHTWFVTFGLWRADNKSQLLMDDRHRHAAMISTRGRMGEAAQVTSVSSFKAD
                     MALEDPRFLDIVAEPWWGGPTAVMTTIFPSVVFQQQVNSVSTRHIQPDGHGAFDFVWT
                     HFGFADDTPEMTERRLRQANLFGPAGFVSADDGEVIELSQQGFEQKPFHRTLVELGGT
                     GVADADHMVTETLIRGMYEYWRGVMEA"
     gene            1979..>2179
                     /locus_tag="VFF72_00385"
     CDS             1979..>2179
                     /locus_tag="VFF72_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salicylate hydroxylase"
                     /protein_id="HZV91637.1"
                     /translation="MPPRIDFQTYHELVQLHADYAIAVDSGTWGLWPEFFVDACSYRL
                     VPRENHERGLPLATLAFESKKML"
BASE COUNT          365 a          736 c          731 g          347 t
ORIGIN      
        1 ttgaggaacg agccgccgaa ggcgcccatg tagaaagcgt cggccagcgt gaagcgacgg
       61 gcgacttccc agagcgccat gtgccgaccg tcgtagtggc ccatcgacag ggcgccggcg
      121 tccgaatacg cggcgaagcg atcgttgcga ccgccgtcga tctgcatgat gttgttgtag
      181 aagcggtgca cgaggtcgcg cgtcgtgacg gtcgtcggca cggcgatgcc gtgtccgtcg
      241 atcgcgttgg ggccgtcgat ctggaacggc tggttcggca tgccgacggt ctgcgcctgc
      301 gtcaccacct gcgcctggcc ggcggccgtc aggccacccc agaccggcgg cagaaccggc
      361 agcacggcgc cgtcgacgtc gcgttgcggc gcgatcgcgc caatggccga cggattcagc
      421 cccggaatgc cgttggcgcc ggggaacagg ccgtagaagg tgtcgaacga ccggttctcg
      481 gcatagatga cgacgacggt atcgatcgcg tcgatggccc ggcgggcgct ggccggcgca
      541 ggcgacagcg tcggcgccga agcgcacgaa ccgagaagaa cgacggcgac gagagcaagc
      601 gcggtggagg gcttcatcgg agcgaccggc cgggacgaag cggcgattgt cgttcggcgg
      661 gccgggacgc gagccgatct ggtttacgct gtgcagtcac gcacgccgcc caccatgcct
      721 gccgacctga ccgccgaagc cgttcccccg agttggcccg gccccggcac cagccgcatt
      781 cccttcgccg tctacaccag cgaatcgctg cacaggcgcg agctcgagcg cttcttctac
      841 cgcggccact ggtgctacgt cgggctcgaa gccgaagtgc ctcgacccgg cgatttcaag
      901 cgcacggtca tcggcgaacg gtcggtcatc ctggtccgcg ccgacgacgg cagcctgaat
      961 gtcgtcgaga acgtctgcgc ccatcgcggc atgagctttt gccgggagcg ccacggcaat
     1021 cgcaaggatt tcacgtgtcc ctaccaccag tggaactatt cgctcaaggg cgatctccag
     1081 ggcgtgccct ttcggcgcgg cgtccgccac gaaggcatcg tgcagggcgg catgccggcc
     1141 gacttcaagc cccaggaaca cggtctgacg aaactgcgcg tcgcggcgcg tggcggcgtc
     1201 gtcttcgcct cgttcgatgc cgaggtcgag tcgctcgagg actttctcgg gccgaccatc
     1261 ctgcgctact acgaccgcct cttcaacgga cgcgagctgc gccttctcgg ctacaaccgc
     1321 cagcgcattc cgggcaactg gaagctgatg caggaaaaca tcaaggatcc gtaccacccg
     1381 ggcctgctgc atacctggtt cgtcaccttc ggcctttggc gggccgacaa caagtcgcaa
     1441 ctgctgatgg acgaccggca tcgccacgcg gcgatgatct cgacccgtgg ccgcatgggc
     1501 gaggcggcgc aggtcacgtc ggtgtcgagc ttcaaggccg acatggccct cgaggatccg
     1561 cgcttcctcg acatcgtcgc cgaaccctgg tggggcggcc cgacggcggt gatgaccacg
     1621 atctttccga gcgtcgtctt ccagcagcag gtcaattcgg tgtcgacgcg gcacatccag
     1681 cccgacggcc acggcgcgtt cgatttcgtc tggacccact tcggcttcgc cgacgacacg
     1741 cccgagatga ccgagcggag gttgcgccag gccaacctct tcgggcccgc cggcttcgtc
     1801 tccgccgacg acggcgaggt gatcgagctg tcgcagcagg gcttcgagca aaagcccttt
     1861 caccgcacgc tggtcgagct gggcggcacc ggcgtcgccg acgccgacca catggtcacg
     1921 gagacgctga tccgcggcat gtacgaatac tggcgcggcg tcatggaggc ctgaggagat
     1981 gccgccccgg atcgacttcc agacctatca cgagctcgtg cagctgcacg ccgactacgc
     2041 gatcgcggtc gactcgggca cctggggcct gtggcccgaa ttcttcgtcg acgcctgctc
     2101 gtaccggctg gtgccgcgcg agaaccatga gcgcggcttg ccgctggcga cgctggcctt
     2161 cgagagcaag aagatgctg
//
LOCUS       DASRWS010000024         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4039235, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000024 DASRWS010000000
VERSION     DASRWS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039235"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFF72_00390"
     CDS             <1..1013
                     /locus_tag="VFF72_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91638.1"
                     /translation="SAAAILLGSGFALALWNLGRTLWQARPLPLPARFVTIGLLCAGA
                     TVALGIVFALVLDGATDNPRLADLTTYGIPVHAAAGLGGWLTFTALGVSYRLLAMFML
                     APELDSARSRAALYSGTVALAVVILGGTAAICLGRGLNAVFAVSSLLGLCAVALYGAD
                     ILHLYRARKRRKIELNARMAAVALTNLTVLVLLSVALIALGRFETKIGAVVFLTAFGW
                     LTGLGLAKLYKIVAFLTWLECYGPVLGKTATPRVQDLVVESRAAKWFWLYFAGVWSAT
                     ACLLLETPAAFRASAALMLVGTIGIGVQIVRTRLMADVKAELRLSHLTRRPPLLASQQ
                     AH"
     gene            1024..1545
                     /locus_tag="VFF72_00395"
     CDS             1024..1545
                     /locus_tag="VFF72_00395"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HZV91639.1"
                     /translation="MTDFVDVDVRPTLRAGGEPFGQIMAAVNALTPGQGLRLYATFKP
                     TPLLQVLGSKGFGYEAREIGGGDWEVLFTPAGVAKDTAAPTAAADNNEEWPSPVRHLD
                     NRDLDPPEPMVRILAAVEAMQPGEVLTALLCREPVFLFPQLAKRGHAWRGGFEPDGAT
                     YKVLVRIGRPGAA"
     gene            1542..1877
                     /locus_tag="VFF72_00400"
     CDS             1542..1877
                     /locus_tag="VFF72_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HZV91640.1"
                     /translation="MMTSSRQELGQRIEDALRLVIDPELGLNIVDLGLVYVVGVDDSG
                     GARILMTTTTRGCPASAFLKDGAQQAAAAVPGVTSAEVSLTYDPAWKPGMMTPWAREQ
                     LGFALGNDP"
     gene            1874..2098
                     /locus_tag="VFF72_00405"
     CDS             1874..2098
                     /locus_tag="VFF72_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91641.1"
                     /translation="MTRRQTVTAQPEEIPPLLVDILLASLTALAMAGRIETACRLAGQ
                     ACAALRQSSPAAWRAFNVFLHRFAPQIPEP"
     gene            2176..2388
                     /locus_tag="VFF72_00410"
     CDS             2176..2388
                     /locus_tag="VFF72_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HZV91642.1"
                     /translation="MPAKGQSVQADSVVDDIMRTWPSTIRVFLDFHMNCVGCAIGPYH
                     TIRDSCEAHDIEMEAFLVALQKARAA"
     gene            complement(2392..3081)
                     /locus_tag="VFF72_00415"
     CDS             complement(2392..3081)
                     /locus_tag="VFF72_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZV91643.1"
                     /translation="MPHLDHSLVSGLTLFAGLEPVELDDILTESHAVHRRKNTTVFEQ
                     GEEAHSFFVLLHGHIQAAKVTPGGQQVVVRYVTPGETFGVAVAIGLSTYPATATAVDD
                     SLVLAWPSAAWPRLVAKYPVIAAKTLQTVGERLQESHTRIIDISTEQVERRVARALLR
                     LIRQAGRTTEKGIEIDFPISRQDIAEMTGTTLHSVSRILSAWEDQGVVAGGRQRILVC
                     DPHKLARIGDE"
BASE COUNT          537 a         1074 c          999 g          657 t
ORIGIN      
        1 tctctgccgc ggccattctg ctcggctcag gatttgcgct ggccctctgg aatctcggcc
       61 ggacgctgtg gcaggcgcgc ccactcccgc tgcccgcccg gttcgtcacc attggcctcc
      121 tgtgcgccgg agcaaccgta gcgctcggca tcgtctttgc attggttctg gatggagcga
      181 ctgacaatcc gcgcctcgcc gacctcacca cgtacggaat tcccgtgcac gcagcagccg
      241 gcctcggcgg ctggctgacc ttcacggccc tcggcgtcag ttatcggctc ctggccatgt
      301 ttatgctggc gccggaattg gatagcgccc gatcgcgcgc agccctgtat tcgggcacgg
      361 tggcattggc ggtggttatc ctgggcggca cggcggccat ctgcctgggc cggggtctga
      421 acgccgtatt cgccgtttcc tcgcttctcg ggctttgcgc cgtcgctctc tacggtgcgg
      481 atatcctgca cctgtaccgc gcccgcaagc gtcgcaagat cgaactcaac gcgcgaatgg
      541 ccgccgttgc actgaccaat ctgaccgttc tcgttcttct gtcggtcgct ctcatcgccc
      601 tcggccgctt cgagacgaag atcggcgccg tcgtctttct cactgccttc ggttggctca
      661 ccggcctggg cctcgcgaag ctctacaaga tcgtcgcctt tctgacctgg ctggagtgct
      721 acggcccggt gctcggcaag acggcgacgc cgcgcgtcca ggacctcgtc gtcgagtccc
      781 gtgccgcgaa atggttctgg ctctatttcg cgggcgtctg gtccgcgacc gcgtgcttgc
      841 tgcttgagac gcctgcagcc ttccgcgcgt cggcggcgct gatgctggtc gggacgatcg
      901 gcatcggtgt gcagatcgtg cgcacgcgcc tgatggccga cgtgaaagcc gaattgcggc
      961 tttcgcactt gacgcgtcgg ccgcccctac ttgcctcgca acaggctcac tgaggagacg
     1021 atcatgacag acttcgtgga tgtagacgta cgaccgacat tgcgcgccgg cggggaacct
     1081 ttcggacaga tcatggcggc cgtaaatgcc ctgacgcccg gtcaaggctt gcgcctctat
     1141 gcgactttca aaccgacgcc gctgctgcaa gtcctgggct ccaaaggatt tgggtatgaa
     1201 gcccgggaaa tcggaggcgg cgactgggag gtgcttttca cgccggccgg cgtggcgaag
     1261 gacacagccg cgccaacagc cgcggccgat aataatgagg agtggccgag tccggttcgc
     1321 cacctcgaca atcgcgacct cgatccgccg gagcccatgg tgcgcatcct tgccgcggtc
     1381 gaagccatgc agcccggcga agtgctcacc gctctgctct gccgcgagcc ggtattcctg
     1441 tttccgcaac tcgccaaacg cggccatgcc tggcgcggcg gcttcgagcc agacggcgcg
     1501 acctacaagg ttctggtgcg gatcggacga ccgggggcag catgatgacc tcttcccgtc
     1561 aggaactcgg ccagcgcatc gaagatgcgc tgcgtctcgt catcgatccc gaacttgggc
     1621 tcaacatcgt cgacctcggg ctggtttacg tcgtcggggt ggacgacagc ggcggcgcgc
     1681 gcatcctcat gaccacgacg acgcgtggct gcccggcttc cgctttcttg aaggacggcg
     1741 cgcagcaggc ggccgctgcc gtgcccggtg tgacgtcagc cgaagtttcg ctgacatacg
     1801 atccggcctg gaagccgggg atgatgacac catgggcccg cgagcagctc ggttttgctc
     1861 tgggaaatga tccatgactc ggcgtcagac cgtcacggcc cagccggaag agatcccgcc
     1921 gcttctggtg gatatcttat tggcaagcct gacagcgctg gctatggccg gccggatcga
     1981 gaccgcctgc cggctggccg gacaagcctg cgcagctctg cgccagagtt cgccggcggc
     2041 gtggcgggcg ttcaatgtct ttctgcatcg gttcgcaccg cagatacctg agccctagtt
     2101 cgattgtgcg atcaaatcgt cgccctgctt gctccagcgc aagaaatgaa atttttacct
     2161 gggtaggttc acggcatgcc agccaaggga cagagcgttc aagccgacag cgtcgtcgac
     2221 gacatcatgc ggacatggcc atcgacgatc cgcgtgtttc tcgattttca catgaattgc
     2281 gtgggctgcg ccatcgggcc gtaccacacg atccgtgact cttgcgaagc acacgacatc
     2341 gagatggagg ccttcctggt tgccctgcag aaggcccgtg ccgcctaggc tctactcgtc
     2401 gccgatccgc gccagcttgt gcggatcgca caccaggatt ctctgccgcc ctcccgccac
     2461 cacgccctga tcttcccagg cgctcaggat tcggctgacg ctgtgaagcg tcgtgccagt
     2521 catctcggcg atgtcctggc ggctgatggg gaaatcgatc tctatgccct tttccgtggt
     2581 gcgccccgcc tgtcggatca gtcgcagcag cgctcgcgcc acgcgccgct cgacctgctc
     2641 ggtcgagata tcgatgatgc gcgtgtgcga ctcctgcaag cgttctccca ctgtctgcaa
     2701 tgtcttcgct gcaatcactg gatatttcgc aaccaggcgc ggccacgcgg ccgacggcca
     2761 cgccaggacg aggctgtcgt cgacagccgt ggcagtggcc ggataagtgc tcaggccgat
     2821 cgccacagcg actccgaagg tctctccggg cgtgacatat ctcaccacga cctgctgacc
     2881 gcccggcgtc accttcgccg cctgaatgtg cccatgcagc agcacaaaaa acgagtgcgc
     2941 ctcctctcct tgctcgaaca ccgttgtgtt cttgcgccga tgcactgcgt gcgattccgt
     3001 gaggatgtcg tcgagttcaa ccggctccaa gcccgcaaac aatgtcaggc cggagacgag
     3061 cgaatgatcc agatgaggca tggctattga tatttctggc tgccgctttg gcaggaggca
     3121 ccaagtatag aaggagtttg tcgcgccggt acgacatccg gccagttcga atcccgcatc
     3181 agaacaggaa atccatacgc ttctaccgcc gcatcatcaa acatggtttc gcctgggtag
     3241 cccctggttt gtcaggacgt cgcgtgg
//
LOCUS       DASRWS010000025         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3463001, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000025 DASRWS010000000
VERSION     DASRWS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3463001"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /gene="queG"
                     /locus_tag="VFF72_00420"
     CDS             complement(<1..702)
                     /gene="queG"
                     /locus_tag="VFF72_00420"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZV91644.1"
                     /translation="MNESRMVDGETLVAQLRDWGRELGFSQIGIADIDLVSGERDFLA
                     WLGDGFHGSMDYMAGHGTKRSRPAELVPGTVRVVTARLDYLPAGTGADWREVEWHRLE
                     AEPERAAISIYARGRDYHKVSRARLRTLADRLADAIGPFGHRVFTDSAPVLEVELAAR
                     SGIGWRGKHTLALDRDAGSTFFLGEIYVDVALPTTPPVEPCCGSCSACIDVCPTAAIV
                     APYRLDARRCISYLTI"
     gene            729..1187
                     /gene="tsaE"
                     /locus_tag="VFF72_00425"
     CDS             729..1187
                     /gene="tsaE"
                     /locus_tag="VFF72_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZV91645.1"
                     /translation="MQAWPDEAACGSAAIALADKPGLANAFIELHGPLGAGKTTFVRH
                     LLRALGVEGPVKSPTYALLESYRAAAGHGGFEIAHFDFYRFDDPGEWEDAGFREVFAA
                     AGLKLVEWPEKAAALLPRCDLRIDIAPDAADEGRTVRFEALSRTGQELLR"
     gene            1220..2572
                     /locus_tag="VFF72_00430"
     CDS             1220..2572
                     /locus_tag="VFF72_00430"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZV91646.1"
                     /translation="MGALALAFGVRGVVQAASIIAVRVWPADEYTRITIESDAALSQH
                     HFIAENPSRLVIDVEGLELSPQLRDLVGKVRPDDPFIAGVRVGQNQPRVVRLVIDLKQ
                     PIAPQLFALDPVAAYRYRLVFDLYPTHERDPLLALIRDKERAEAKAARDVQDALGEFI
                     GRIDAGVPAASTPEGAETAPRLARNVPRLDTLPRPPTLAPPIPEAPPPGSQAKIDRLV
                     VVAIDPGHGGEDPGATGPSGLHEKDVVLAIALKLRDRLNAVPGMRAMLTRDADYFVPL
                     GERVRKARRVQADLFVSIHADAFMTPEARGASVFALSQSGATSSAARWMAKRENAADL
                     VGGVNIATKDQSVMRALLDMSTTAQIKDSLKLGGEVLGQIGKVGPLHKGSVEQAGFAV
                     LKAPDIPSILVESAFISNPQEEAKLRDPEYRDRLVEALATGIRRYFARNPPLARSRQL
                     "
     gene            2620..3564
                     /locus_tag="VFF72_00435"
     CDS             2620..3564
                     /locus_tag="VFF72_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV91647.1"
                     /translation="MLHPQARALLDLIEQRGLPTMQSLSPAEARRFYRDRRSLTQPDP
                     PAVGEVTELSARGPHGAIPLRLYRPIGAGDRARAQPVLVYFHGGGWTIGDLDTHDVLC
                     RELANGSDCAVVSVEYRMGPEHRFPAAVDDCLAATRWVSAQAGALGLDATRLAVGGDS
                     AGGNLAAVVAIAARDAGNLPIAFQLLIYPATDMRRGHASHVVNGRGYLLESETMAYYH
                     DHYIDDPRHDADWRASPLLHPDLSKLPPALVVTAGFDPLRDEGLAYADALVATGNAAS
                     YVCFERQIHGFITMGRLLDEADTAVGLCAAETARWLRR"
     gene            complement(3602..3883)
                     /locus_tag="VFF72_00440"
     CDS             complement(3602..3883)
                     /locus_tag="VFF72_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91648.1"
                     /translation="MTPRLVVASLALAAAALSLPSFGAESVGQAASTAANETGHAIGT
                     AGRATGHAVAEAGRETGHAVAKAGRKTGHAVHKGATRTRNAVKGASSPG"
     gene            4079..>4349
                     /locus_tag="VFF72_00445"
     CDS             4079..>4349
                     /locus_tag="VFF72_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91649.1"
                     /translation="MKTTLKMAIVAALAATATMTMPLAAQARGAGGGGWHGGGHHGGG
                     WHGGGWHGGYRGGHGWHGGYWGPGPFWGGLGLGLGLGAIAYYGAYG"
BASE COUNT          636 a         1562 c         1518 g          633 t
ORIGIN      
        1 gatcgtcagg tacgagatgc agcgcctcgc gtcgagccgg tacggggcga cgatggcggc
       61 ggtcgggcag acgtcgatgc aggcgctgca gctgccgcag cagggctcga cgggcggcgt
      121 cgtcggcaac gccacgtcga catagatctc gccgagaaag aaggtcgagc cggcgtcgcg
      181 atcgagcgcc agcgtgtgct tgccgcgcca gccgatgccg cttctggcgg cgagctcgac
      241 ctcgagcacc ggcgccgagt cggtgaagac gcgatgtccg aacggcccga tggcatcggc
      301 gagccgatcg gccagcgtgc gaagccgagc acgcgacacc ttgtgatagt cgcgaccgcg
      361 tgcgtagatc gagatcgccg cgcgctcggg ctcggcctcg aggcgatgcc actcgacctc
      421 gcgccagtcg gccccggtgc cggcgggcag gtagtcgaga cgcgccgtga cgacacgcac
      481 ggtgcccggc accagctcgg cgggccggct ccgcttcgtg ccgtgtccgg ccatgtagtc
      541 catgctgccg tgaaagccgt cgccgagcca ggcgagaaaa tccctttccc ccgacaccag
      601 gtcgatatcg gcgatgccga tctgggaaaa tccgagctcg cggccccagt cgcgcaactg
      661 cgctacgagg gtttcgccat cgaccatccg ggattcgttc attgcaggca acgattctag
      721 aaacgagcat gcaggcgtgg cccgacgagg cggcttgcgg ttcggcggcg atcgcgctcg
      781 cggacaagcc cggtctcgcc aacgccttca tcgagctgca cggtccgctc ggcgccggca
      841 agacgacctt cgtgcgccat cttctgcgcg cgctcggcgt cgaagggccg gtgaaaagcc
      901 ccacctacgc cttgctcgag tcgtatcgag cggcggccgg tcatggcggc ttcgagatcg
      961 cgcatttcga tttctaccgc ttcgacgatc ccggcgagtg ggaggacgcg ggctttcgcg
     1021 aggtcttcgc ggcggccggc ctcaagctcg tcgaatggcc cgagaaggcg gcggcgctgc
     1081 tgcctcgctg cgatctgcgc atcgacatcg cgccggacgc ggccgacgaa ggccgcaccg
     1141 tgcgcttcga ggcgctcagt cgcaccggcc aggagttgct gcgttgagct cgccctcgcg
     1201 tcgccgggcc ctgagccgca tgggcgcgct ggcgctggcc ttcggcgtgc gcggcgtggt
     1261 gcaggccgcg tcgatcatcg ccgtccgcgt ctggccggcc gacgaataca cccgcatcac
     1321 gatcgagtcc gacgcggcgc tgtcgcagca ccacttcatc gccgaaaacc cgtcccgcct
     1381 ggtgatcgac gtcgaaggcc tcgagctcag cccgcagctg cgcgacctcg tcggcaaggt
     1441 acggcccgac gaccccttca tcgccggggt tcgcgtcggc cagaaccagc cgcgggtcgt
     1501 ccgcctcgtc atcgacctga agcagcccat cgcgccgcag ctctttgcgc tggacccggt
     1561 cgcggcctat cgctaccgcc tcgtcttcga cctctacccg acgcacgagc gcgatccgct
     1621 gctggcgctg atccgcgaca aggaacgggc cgaggcgaag gccgcacgcg acgtgcagga
     1681 cgcgctcggc gaattcatcg gccgcatcga cgccggtgtt ccggccgcgt cgacgcccga
     1741 gggcgccgag acggcgccgc ggctcgcccg caacgtcccg cgcctcgaca cgctgccccg
     1801 gccaccgacc ctggcgccgc cgattcccga agcgccgccg ccgggcagcc aggcgaagat
     1861 cgatcggctc gtcgtcgtcg ccatcgatcc ggggcacggc ggcgaggatc cgggtgcgac
     1921 cggacccagc ggcctgcacg agaaagacgt cgtcctggcc atcgccctga agctgcgcga
     1981 tcgcctcaat gccgtccccg gaatgcgggc gatgctgacc cgcgacgcgg actatttcgt
     2041 gccgctcggc gagcgcgtcc ggaaggcgcg ccgcgtccag gccgacctgt tcgtctcgat
     2101 ccacgccgac gcgttcatga cgccggaggc ccgcggcgcg tcggtgttcg ccttgagcca
     2161 gagcggcgcg acgagcagcg cggcgcgctg gatggccaag cgcgagaacg cggccgacct
     2221 cgtcggcggc gtcaacatcg ccaccaagga ccagtcggtg atgcgcgcgc tcctcgacat
     2281 gagcaccacc gcgcagatca aggacagcct caagctcggc ggcgaggtgc tcggccagat
     2341 cggcaaggtc ggaccgctgc acaagggcag cgtcgagcag gcgggattcg cggtgctgaa
     2401 agcgcccgac atcccgtcga tcctggtcga gtcggccttc atctcgaacc cgcaggaaga
     2461 agcgaagctg cgcgaccccg agtaccgcga ccggctcgtc gaagcgctgg cgaccggcat
     2521 ccggcgctac ttcgcccgca acccgccgct ggcacgcagc cgccagctct gacgcccgca
     2581 gctcccgcgc gaacgtcgcc gaccccgtca ggacacgcca tgctccatcc gcaagcccgc
     2641 gccctgctcg acctgatcga gcagcgtggc ctgccgacga tgcaatcgct ctcgcccgcc
     2701 gaggcgcggc ggttctaccg cgaccgacgc tccctgacgc aacccgatcc gccggcggtg
     2761 ggcgaagtga ccgagctgtc ggcccgcggt ccgcacggcg ccatcccgct gcgcctgtat
     2821 cgacccatcg gcgcgggcga ccgggcccgc gcgcagccgg tgctcgtgta ctttcacggc
     2881 ggtggctgga cgatcggcga cctcgacacc cacgatgtgc tttgccggga gctcgccaac
     2941 gggtcggact gcgccgtcgt ctcggtcgag taccggatgg ggccggagca ccggtttccg
     3001 gccgcggtcg acgattgcct cgcggcgacg cgctgggtga gtgcgcaggc cggcgcgctg
     3061 ggtctcgacg cgactcggct ggccgtcggc ggcgacagcg ccggcggcaa tctcgctgcc
     3121 gtcgtcgcca tcgcggcgcg cgacgccggc aacctgccga tcgcctttca gcttctcatc
     3181 tacccggcca ccgacatgcg ccgcggtcac gcgtcgcacg tcgtcaacgg acgcggctat
     3241 ctgctcgagt ccgaaaccat ggcctattac cacgatcact acatcgacga tccgcggcac
     3301 gacgccgatt ggcgagcctc gccgctgctg caccccgatc tgtcgaagtt gccgccggca
     3361 ctcgtcgtca ccgccggctt cgatccgctg cgagacgagg gcctggccta tgccgacgcc
     3421 ctcgtcgcca ccggaaacgc cgcgagctac gtctgcttcg agcgccagat ccatggtttc
     3481 atcaccatgg gccgcctcct cgacgaggcc gacaccgctg tcggcctgtg cgccgcggag
     3541 acggcaaggt ggctgcgccg ctgaacgctg cgcagcgacg ctggcgagcg gcctgaaggg
     3601 gtcagcccgg cgacgaagcg cccttcaccg cgttcctggt cctggtcgcg cccttgtgca
     3661 cggcgtggcc ggtcttgcgg ccggccttgg ccacggcatg acctgtttcg cggccggctt
     3721 cggcgacggc gtggcccgtg gcacggccgg cggtcccgat cgcgtggcct gtttcgttgg
     3781 cggcggtcga tgcggcctgg ccgaccgact cggcgccgaa cgacggcagc gacagcgctg
     3841 cggcggcaag cgcgagcgat gcgacaacga ggcgtggggt catgacattc ctccggatga
     3901 cttcgacgaa aaatgatatt cgccggtgcg ccgagggcgg cttctccggc gtgctggccg
     3961 cttgtcggac ttcaggccgg gatgccactg gccctgtccc ggcattgccc gccgggcgca
     4021 aaatgcatca tctgccgaat gggcgcgaca gcgcgtggcg gccgcaggag acgacgaaat
     4081 gaagacgacc ttgaagatgg cgatcgtggc ggcgctggct gccacggcga cgatgacgat
     4141 gccgctcgcc gcgcaggcgc gcggcgcggg tggcggcggc tggcacggcg gcgggcacca
     4201 cggcggcggc tggcatggcg gcggatggca tggcggatac cgcggtggcc acggctggca
     4261 tggcggctac tgggggccgg ggccgttctg gggcggtctg gggctcggcc tgggcctggg
     4321 ggctatcgcg tactacggcg cttacggcc
//
LOCUS       DASRWS010000026         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_301931, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000026 DASRWS010000000
VERSION     DASRWS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301931"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2464
                     /locus_tag="VFF72_00450"
     CDS             <1..2464
                     /locus_tag="VFF72_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91650.1"
                     /translation="PTITQQPADLTVTAGQGATFTVAATGTAPLSYQWQRDNVDIAGA
                     TSTTYDVPSTVIGDSGATFRAVVSNVAGSATSNNATLTVTVSAPVLTISPQPASTSVV
                     AGANASFTVGGTCSSGTLDVQWQRNSGAQGAFVAIAGATAATYSFMTVLADSGAQFRA
                     VLDCSGQSTTPSDAATLTVTAPGSVTLSLLPLNGLRPQATISSATAVDQEPSGTYAFS
                     TFNRIKRLSVDLLTIDLLAGDTWQGSADGAAATARFNGPRGITHDVAGNLYVADTGNH
                     TIRRIAADGTVSTIAGSPGVSGSADGSGNAARFDMPWGIALGPDGDLYVADSGNNLIR
                     RVTTAGVVTTYAGSTSGFLDGAPLTAQFRTPAGVAVAANGDVLVADASNNRVRRIVRS
                     GNVAGSVETLAGTGTYSTTPPDGIGTAAGISDPIALYVRGNILYVREYIGLIRQVDLT
                     TKAVTTLAGSRTLGAGYADGPAGAARVRDGLGGITGAPGGAFITVEDVAVRLVSAAGD
                     ITTLADDAAIGQTDTGVGTLPQQPFGLAINDYASLGVDAAGRVVVADSDTNEVRRIDN
                     TGAVTLAAGLTGSFGGVVDGKGSAAQFVDLGISLATSPTGVTYVGGSYGVRRIAADNT
                     VTTLAGSTTMFGGVDGDATTARFSRIFGLAVGPNGDVFAADAGNSAVRRIDTAGNVTT
                     YAGVIGQGGSVDGPIASARFLGPRAVAFGPDGSLYVADDGRVRKISPDGSTVSTISAA
                     GGLVQTITVDAADTLYYYDQAGLSMIPAGATTNTLLIPATTGNNVLGASPRLFSVQSM
                     AMLGPKQIVIVAASQLLVATLP"
     gene            2549..>2831
                     /locus_tag="VFF72_00455"
     CDS             2549..>2831
                     /locus_tag="VFF72_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HZV91651.1"
                     /translation="MATRLLLGVLWAFQFLPLALQAACGRALGQAGWILVPQRRKVAL
                     RNLELCFPEWRAAERERVAREHFRWLGRSLLERGLLWYASPERLKRLIHV"
BASE COUNT          422 a         1072 c          944 g          393 t
ORIGIN      
        1 gccgacgatc acccagcaac cggccgacct cacggttacc gccggacagg gcgcgacctt
       61 caccgtcgcg gcgaccggca ccgcgccgct cagctatcaa tggcagcgcg acaacgtcga
      121 cattgccggc gcgacgtcga ccacctacga cgtgccgagc acggtgatcg gcgacagcgg
      181 cgcgacgttt cgcgcggtcg tcagcaacgt cgccggcagc gcgaccagca acaacgcaac
      241 gctcacggtg acggtgagcg cgccggtcct gacgatctcg ccgcagccgg ccagtaccag
      301 tgtcgtcgcc ggggcgaacg cctcgttcac ggtgggcggc acctgcagca gcggcacgct
      361 cgacgtgcag tggcagcgca actccggcgc gcagggtgcg ttcgtcgcca tcgccggcgc
      421 taccgccgcg acctacagtt tcatgaccgt gctcgccgac agcggcgccc agttccgcgc
      481 cgtgctcgac tgcagcggcc agagcacgac gccgagcgac gcggcgacgc tgaccgtgac
      541 cgcgccgggc tcggtcacgc tcagcctgct gcccctgaac ggcctgcgcc cccaggcgac
      601 gatcagcagc gcgacagccg tcgaccagga accctcgggc acgtacgcct tcagcacctt
      661 caaccgcatc aagcgcttga gcgtcgatct gctgaccatc gatctgctcg ccggtgatac
      721 gtggcaaggg tcagccgatg gcgccgccgc aacggcgcgc ttcaacgggc cccgtggcat
      781 cacgcacgac gttgccggca acctgtacgt cgccgacacc ggcaaccaca cgatccggcg
      841 gatcgcggcc gatggcacgg tgagcacgat cgccggctcg ccaggagtca gcggctcggc
      901 cgacggctcc ggcaacgcgg cgcgcttcga catgccttgg ggcatcgccc tcgggccgga
      961 tggcgacctc tacgtggccg actccggcaa caacctgatc cgccgcgtca cgaccgcagg
     1021 cgtggtcacg acctacgccg gcagcacctc gggctttctc gacggggcgc cgctgacggc
     1081 ccagttcagg acgcctgccg gcgtcgccgt cgccgccaac ggcgacgtgc tggtcgcaga
     1141 cgccagcaac aaccgcgtgc gccgcatcgt gcgcagcggc aacgtcgccg gctcggtcga
     1201 gacgcttgcg ggcaccggta cctactcgac gaccccgccc gacggcatcg gcactgccgc
     1261 cggcatcagc gacccgatcg cgctctacgt ccgcggcaac attctctacg tgcgcgaata
     1321 catcggcctg atccggcagg tcgacctcac caccaaggcc gtgacgacgc tggccggctc
     1381 gcgcacgctc ggtgccggct acgccgacgg tcctgccggg gcagcacggg tgcgcgacgg
     1441 cctgggcggc atcaccggtg cgccgggcgg cgccttcatc accgtcgagg acgtcgcggt
     1501 gcgcctcgtg agcgctgcgg gcgacatcac gacgctcgcc gacgacgcgg cgatcggcca
     1561 gaccgacacc ggcgtcggca ccctgccgca gcagccgttc ggcctggcca tcaacgacta
     1621 cgcctcgctc ggggtcgacg cggcgggacg ggtcgtggtc gccgattccg acaccaacga
     1681 ggtccgtcgc atcgacaaca ccggtgccgt cacgctcgcc gccggcctca ccggcagctt
     1741 cgggggcgtg gtcgacggca agggcagcgc tgcgcaattc gtcgatctcg gcatctcgct
     1801 cgccacctcc ccgaccggcg tcacctacgt cggcggcagc tacggcgtgc gtcgcatcgc
     1861 cgccgacaac acggtgacca cgctggccgg ctcgaccacg atgttcggcg gtgtcgacgg
     1921 cgacgcgacg acggctcgct tcagccgcat cttcggcctg gctgtcggtc ccaatggcga
     1981 cgtctttgct gccgacgccg gcaacagcgc cgtgcgccgc atcgacacgg cggggaacgt
     2041 gaccacctat gccggcgtga tcggccaggg cgggagcgtc gacgggccga tcgcctcggc
     2101 ccgcttcctc ggcccgcgtg cggtcgcctt cggccccgac ggctcgctct atgtcgccga
     2161 cgacggtcgc gtgcgcaaga tcagcccgga cgggtcgacc gtgtcgacca tcagcgcggc
     2221 cggcggactc gtccagacga tcacggtcga cgctgccgat acgctgtatt actacgacca
     2281 ggccgggctg tcgatgattc cagcgggggc gacgacgaac acgctcctca tccccgccac
     2341 cacgggcaac aacgtcctcg gagcgagccc gcgcctgttc tccgtgcaat cgatggcgat
     2401 gctcggcccc aagcagatcg tcatcgtcgc cgccagccag ctgctcgtcg ccacgctgcc
     2461 ctgagcattc ccgcgcgctg ccggtaccgg tggcgcgcgg gatactcgct cccgcgcgcc
     2521 atcggggcgc gaagggagca tcgacaaggt ggcaacgcgg ctcttgttgg gcgtgctgtg
     2581 ggcctttcag tttctgccgc tcgcgctcca ggccgcgtgc ggacgcgcgc tcggccaggc
     2641 cggctggatc ctggtgccgc aacggcgcaa ggtcgccctg cgcaacctcg agctctgctt
     2701 cccggaatgg cgcgcggcgg agcgcgagcg cgtggcgcgc gagcacttcc gctggctcgg
     2761 gcgcagccta ctcgagcgcg gcctcctctg gtatgcctcg cccgagcggc tgaagcggct
     2821 gatccacgtc g
//
LOCUS       DASRWS010000027         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2600107, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000027 DASRWS010000000
VERSION     DASRWS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600107"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFF72_00460"
     CDS             complement(<1..721)
                     /locus_tag="VFF72_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZV91652.1"
                     /translation="MPFPTRSPARSQFLRLAPLSFAVALALQAQCGLAQPAQRQTVAP
                     LTTVEPFTIKDIRVEGLQRTDAGTVFAALPFRIGDTYTTDKGAAALRALFATGLFKDV
                     RIDIEQGVAVIIVEERPVIANIDFVGMKEFEKDALIKSLKDFGIGEGLPFDKSLADRA
                     EQELKRQYLTRSLYGAEVVTTVTPQERNRVNVTFTVTEGAPAKIREIRIVGNHVFSER
                     TLKGLFELNDGGWLNFYLKADR"
     gene            complement(730..2100)
                     /gene="rseP"
                     /locus_tag="VFF72_00465"
     CDS             complement(730..2100)
                     /gene="rseP"
                     /locus_tag="VFF72_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZV91653.1"
                     /translation="MITTLLAFLFTLGVLIVVHEYGHYRVAVACGVRVLRFSVGFGRV
                     IWRRQANPQGTEFVLCALPLGGYVRMLDEREAPVPAAELDRAFNRKPLAKRAAIVAAG
                     PAANLILAVALYAVAHWIGVDEPKALLGPPAATSVAARAGLQAGDWVRSWSSDGGEWH
                     VVRSLSDLRWQVTRSLLRGRNIELSVTDPDGSGPHSVSLDLAALAGIEVDAKLMHQIG
                     LDSAWSEPVLGEVKPGGAGAAAGLHAGDRVLSIDGHAIADATQVRDAIRADAQGGTAR
                     PMRWRVERGQRTLDIIVTPKVVFDAGQRIGRIDAFPGQPPQMVKVRYGVVEGFTSAVS
                     QTWQMSSLTVRMLGKMIVGQASLKNLSGPVTIADYAGQSVRLGLAYYLGFLAVVSVSL
                     GVLNLLPLPVLDGGHLMYYLFEAVTGRQVSELWLDRLQRGGVAIMLVMMSLALYNDMT
                     RLLGLQ"
     gene            complement(2097..>2661)
                     /locus_tag="VFF72_00470"
     CDS             complement(2097..>2661)
                     /locus_tag="VFF72_00470"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347863.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZV91654.1"
                     /translation="MGRKISVDSATMMNKALEVIEAHFLFGLRPEQIRVVIHPQSVIH
                     SMVVCRDESVLAQLGTPDMRVPIAHGLAWPDRIASGTPALDWQRLGPLTFEDADLALY
                     PGLGLAWDTLRGPAGSSAVLNAANEEAVSAFLAGTIGFDRIHTVNARTVAALVPPPGA
                     ADSLEGLLALDAEARSRAGQFLKSLRE"
BASE COUNT          461 a          890 c          916 g          394 t
ORIGIN      
        1 agcgatcggc cttcaggtag aaattgagcc agccgccgtc gttgagctcg aagagcccct
       61 tcagcgtgcg ctcggagaag acatggttgc cgacgatgcg gatctcgcgg atcttcgccg
      121 gggcgccctc ggtgaccgtg aaggtgacgt tgacgcgatt tctttcctga ggcgtcaccg
      181 tggtcacgac ctcggcgccg tacaggcttc tcgtcaggta ttgccgcttc agctcctgct
      241 cggcgcggtc ggcgagcgac ttgtcgaatg gaagcccctc gccgatgccg aaatccttca
      301 gcgacttgat cagcgcgtcc ttctcgaatt ccttcatgcc gacgaagtcg atgttggcga
      361 tgacgggccg ctcctcgacg atgatcacgg cgacgccttg ctcgatgtcg atccgcacgt
      421 ccttgaaaag gccggtcgcg aagagcgcgc gcagcgccgc ggcgcccttg tcggtggtgt
      481 acgtatcgcc gatgcgaaac ggcagcgccg caaacaccgt gccggcgtcg gtgcgctgca
      541 gcccttccac ccggatgtcc ttgatggtga agggctcgac ggtcgtgagg ggagccaccg
      601 tctgccgctg cgcaggctgg gccaggccgc actgagcctg cagcgccagc gccacggcga
      661 aagacagggg agcgaggcga aggaactgcg agcgcgccgg cgagcgtgtc ggaaaaggca
      721 tgcggtcggt cattgaagac ccagcaagcg ggtcatgtcg ttatagagag ccagggacat
      781 catcaccagc atgatggcaa ccccgccccg ctgcagccgg tcgagccaca actcggagac
      841 ctgacgtccc gtcacggcct cgaaaagata atacatcagg tgtccaccgt cgagcacggg
      901 cagaggcagc agattgagaa cgccgagact gacgctgacc actgcgagaa agccgagata
      961 gtaggccagc cccaggcgga ccgactggcc ggcgtagtcg gcgatcgtca ccgggccgct
     1021 caggttcttc agcgacgcct ggccgacgat catcttgccg agcatcctca ccgtcagcga
     1081 cgacatctgc caggtctgcg aaacggccga ggtgaagccc tcgacgacgc catagcggac
     1141 cttgaccatc tgcggcggct gcccggggaa ggcgtcgatg cggccgatcc gctggccggc
     1201 gtcgaagacg accttcggcg tgacgatgat gtcgagcgtt cgctggccgc gctcgacgcg
     1261 ccagcgcatc ggccgcgccg tgccgccttg cgcgtcggca cggatcgcat cgcgcacctg
     1321 cgtcgcgtcg gcgatcgcgt ggccgtcgat cgagagcacc cggtcgcccg cgtgcaatcc
     1381 ggccgcggcg ccggcgccgc cgggcttgac ctcgccgagc accggctcgc tccaggcact
     1441 gtcgagaccg atctggtgca tcagcttggc gtcgacctcg atcccggcga gagcggcgag
     1501 gtcgagcgag acgctgtgcg ggccgctgcc gtcgggatcg gtcactgaaa gctcgatgtt
     1561 ccggccacgc agcagcgatc gcgtcacctg ccagcgcaga tcgctgagcg aacgcacgac
     1621 gtgccattcg ccgccgtcgc tcgaccagga gcggacccag tcgcccgcct gcaggccggc
     1681 gcgcgcggcc acgctcgttg ccgccggcgg gcccagcagg gccttcggct cgtcgacacc
     1741 gatccagtgc gcgacggcgt agagggcgac cgcgaggatc aggttggcgg ccggccccgc
     1801 ggcgacgatg gcggcacgct tggccagcgg cttgcggttg aaggcccggt cgagttcggc
     1861 ggcgggcacc ggcgcctctc gttcgtcgag catgcgcaca tagccgccca gcggcagagc
     1921 gcagaggacg aactccgtgc cctgcggatt cgcctggcgc cgccagatca ctcgaccgaa
     1981 gcccaccgag aagcgcagca cacggacacc gcacgcgacg gcgacacggt agtgcccgta
     2041 ctcgtgcacg acgatcagca cgccgagcgt gaacaggaat gcgaggaggg tcgtgatcat
     2101 tcgcgaaggc tcttcaggaa ctggccggcc cgggatcggg cctcggcatc gagtgcgagc
     2161 aggccctcga gcgagtctgc cgcaccgggc ggggggacga gcgcggcgac ggtgcgcgca
     2221 ttgacggtgt gaatccggtc gaagccgatg gttccggcca gaaacgccga caccgcctcc
     2281 tcgttcgcgg cgttgagcac ggcactgctg cccgccgggc cacgcagcgt gtcccaggcc
     2341 aggccgagac cgggatagag cgcgagatcg gcgtcctcga acgtgagagg gcccaggcgc
     2401 tgccagtcga gcgcaggcgt gcccgaggcg atgcgatccg gccacgccag gccgtgcgcg
     2461 atcggcacgc gcatgtcggg cgtgccgagc tgggcgagaa ccgattcgtc gcgacacacg
     2521 accatcgaat ggatcacgct ctgcggatga atgacgacac ggatctgctc gggcctgagt
     2581 ccgaacagga aatgcgcctc gatcacttcg agcgccttgt tcatcatcgt cgccgagtcg
     2641 acggagatct tgcggcccat c
//
LOCUS       DASRWS010000028         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2313663, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000028 DASRWS010000000
VERSION     DASRWS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313663"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1534
                     /locus_tag="VFF72_00475"
     CDS             185..1534
                     /locus_tag="VFF72_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZV91655.1"
                     /translation="MESRASALNPPPQAAARHAALRGLVEDDRVHRDLYLSEEIFALE
                     QRHFFADTWSYAGHASEVPDAGDYVTREIAGLPLVMVRQPDGGIRVLYNRCAHKGTPL
                     VTDPSGNVGRHFRCPYHAWTYRLDGAPMGVPLKAGYDGTRLRDCESGRGMTALRHVAV
                     HRDFVFVKPGDGGLDFESYFGSALAAIDNLADRSPEGRLEIAGGCLRNVIRCNWKIYL
                     ENINDTVHPMSTHRSAVDAARGVWGGHPADAQPPMAMEQIMPFGAGYEFFDRMGGRAY
                     PNGHSVLGTHFSLHSGYGKVGDYEAAMREAHGAERAAEILERSPQNATLYPSLVVKGS
                     PQAIRVIRPLAVDRTLIEAWAFRVVGAPDLLLERSLGYNRLVFSPMSVVAHDDVHLFE
                     NIQRGLRSGGNPWISLHRGFRSDELAGHEIETSGTDELLMRNQYRAWARFMGEGAPG"
     gene            1611..2156
                     /locus_tag="VFF72_00480"
     CDS             1611..2156
                     /locus_tag="VFF72_00480"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZV91656.1"
                     /translation="MSVAAALERELADFVAAEAALLDDGRFDDWLALFADDGVYWVPL
                     AGARQADPHRHHSIAYEDRALLQLRVARLKDPQAHSQHPPSTCQHVLQRPVLVAADEA
                     AAQPPSREGHAESLLSHAGETHRLRTPFLYVEARGDDELMLAGTCRHRLSRIDGAWKI
                     REKRVDLLNPGRPLPAIQLFI"
     gene            2166..2717
                     /locus_tag="VFF72_00485"
     CDS             2166..2717
                     /locus_tag="VFF72_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HZV91657.1"
                     /translation="MPALHERLRFDTAAGQVLDGPRRYVLLRTDVLAGVFAGLAGPER
                     ETALRAFGRAVETRGADSLRAYAAEPGVDADALQRVVEAAAASLGWGRWHIERRGDAL
                     ELEVGNSPFASAAPPGDGPACAAIAGMLQGLAAIVLGGPASARETSCACVAGGDRCRF
                     AAHLNSSLPADPPVSVHLQETSS"
     gene            2714..>3485
                     /locus_tag="VFF72_00490"
     CDS             2714..>3485
                     /locus_tag="VFF72_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91658.1"
                     /translation="MKTFFRIAAGAALAAGVVASATAADLKVGLVTALAGPASSIGIP
                     YSKGMKAAQAFMPEVGGHKVDLIILDDGSDPATAARNARKLIEEDKVDVLIGSAGVPS
                     SMAMAAVSREAKVPMIALTPIGLAGPEGAWVVAIPQPTGLMVDAVIDQMKKAGVKTVA
                     YIGFSDAWGDLVYNALIKGTEGTGIKVVTNERYARPDTSVSGQILKILATHPDAVMTG
                     GSGTPGALPYLELAKRGYKGRIYGNHGLINPDFVRVAGD"
BASE COUNT          516 a         1296 c         1173 g          500 t
ORIGIN      
        1 ctccagggaa aggcgcgggg aatcggggct tggcgcggac gaatccatgt tcgatggcgc
       61 catcgtagag ccttgccgcg ccgaagcgcc ctggcgcggc ccgcgagcgc cccggtcgag
      121 gcgggagacg cccgattgac gcgcctcgcc gcgacccata tgcttgacaa ctgaagcatt
      181 gccgatggaa tcacgcgcct ccgccctcaa tccgccgccg caggccgccg cgcgacatgc
      241 cgcgcttcgc ggcctcgtcg aggacgaccg cgtccatcgc gacctgtacc tgagcgagga
      301 gatcttcgcc ctcgagcagc gtcatttctt cgccgacacc tggagctacg caggccacgc
      361 gagcgaggtg cccgacgccg gcgactacgt cacccgcgag atcgccggcc tgccgctggt
      421 catggtgcgc cagccggacg gcggcatccg cgtgctctac aaccgctgcg cgcacaaggg
      481 cacgccgctc gtcaccgacc cgagcggcaa cgtgggccgc cacttccgtt gcccgtacca
      541 cgcctggacg taccggctcg acggcgcgcc gatgggcgtg ccgctgaagg ccggctacga
      601 cggcacgcgg ctgcgcgact gcgaatcggg gcgcggcatg acggcgctgc gccacgtcgc
      661 cgtgcaccgc gacttcgtct tcgtcaagcc gggcgacggc ggcctcgatt tcgagagcta
      721 cttcggaagt gcgctcgccg ccatcgacaa cctcgccgat cgatcgcccg agggtcgcct
      781 cgagatcgcc ggcggctgcc tgcgcaacgt catccgctgc aactggaaga tctacctcga
      841 gaacatcaac gacacggtcc atccgatgtc gacgcaccgc tcggccgtcg acgccgccag
      901 gggcgtgtgg ggcggccatc ccgccgatgc gcagccgccg atggcgatgg agcagatcat
      961 gccgttcggc gccggctacg agttcttcga tcgcatgggc ggccgcgcct atccgaacgg
     1021 ccacagcgtc ctcggcaccc atttcagcct gcattcgggc tacggcaagg tcggcgacta
     1081 cgaagcggcg atgcgcgagg cgcacggcgc cgagcgcgcc gccgagatcc tcgagcgctc
     1141 gccgcagaac gcgacgctct atccgagcct ggtggtgaag ggctcgccgc aggcgatccg
     1201 cgtcatccgg ccgctcgccg tcgaccgcac cctgatcgag gcgtgggcgt ttcgcgtcgt
     1261 cggcgcgccc gacctgctcc tcgagcgatc gctcggctac aaccgcctcg tcttttcgcc
     1321 gatgtcggtg gtcgcgcacg acgacgtcca tctcttcgag aacatccagc gcgggctgcg
     1381 ctccggcggc aacccgtgga tcagcctgca tcgtggcttc aggtcggatg agcttgccgg
     1441 gcacgagatc gaaaccagcg gcaccgacga gctcctgatg cgaaaccagt accgcgcctg
     1501 ggcgcgattc atgggcgaag gggcgccagg atgagcgcgt ccgcccgagc gcaccgcacg
     1561 ccatgccccg ggccgcccgg cgaggccggt tgccggccga aggtgcgcgc gtgagcgtcg
     1621 ccgccgcgct cgagcgcgag ctcgccgatt tcgtcgccgc cgaggcggcg ctcctcgacg
     1681 acgggcgctt cgacgactgg ctggcgctct tcgccgacga cggcgtctac tgggtgccgc
     1741 tggcgggtgc gcgccaggcc gacccgcatc gccatcactc gatcgcctac gaagaccgcg
     1801 ccctgctgca gcttcgcgtc gcccggctga aggatccgca ggcgcattcg cagcacccgc
     1861 cgagcacctg ccagcacgtg ctgcagcgtc cggtgctcgt ggccgccgac gaggcggcgg
     1921 cgcagccgcc ttcgcgcgaa ggacatgcgg agagccttct ttcgcacgct ggcgagacgc
     1981 atcgcctgcg cacgccgttt ctctacgtcg aggcgcgcgg cgacgacgag ctgatgctcg
     2041 ccggcacctg ccggcatcgc ctgtcacgca tcgacggcgc atggaagatc cgcgaaaagc
     2101 gcgtcgacct gctcaacccc ggccggccat tgccggcgat ccagctcttc atctgacggc
     2161 gatccatgcc agctctgcac gaacgtctcc gcttcgacac cgccgctggc caggtgctcg
     2221 acggaccgcg ccgctacgtg ctgctgcgca ccgacgtgct cgccggcgtg ttcgccgggc
     2281 tcgccggtcc cgagcgcgag acggcgctgc gtgccttcgg ccgcgccgtc gagacgcgcg
     2341 gcgccgacag cctgcgcgcc tatgcggccg agcccggtgt cgacgccgac gccctgcaac
     2401 gcgtcgtcga ggccgcggcg gcgtcgctcg gctggggccg ctggcacatc gaacgccgcg
     2461 gcgacgcgct cgagctcgaa gtgggcaaca gcccgttcgc gagcgccgca ccgccgggcg
     2521 acgggcctgc ctgcgcggcc atcgcgggca tgctgcaagg cctcgctgcc atcgtcctcg
     2581 gcggcccggc cagcgcccgc gagacgtcgt gcgcctgcgt tgccggcggc gatcgctgcc
     2641 gcttcgcggc gcacctgaat tcctcgctgc cggccgatcc gccggtgtcc gttcacctcc
     2701 aggagacctc gtcatgaaga ctttctttcg catcgccgcc ggcgcggcgc tcgccgcggg
     2761 cgtcgtcgcc tcggccacgg ccgccgacct caaggtcggg ctggtcaccg cgctcgccgg
     2821 accggcgtcg tcgatcggca ttccgtacag caaggggatg aaggcggcgc aagccttcat
     2881 gcccgaagtc ggcggccaca aggtcgatct gatcatcctc gacgacggat ccgacccggc
     2941 caccgcggcg cgcaacgcca ggaagctgat cgaggaagac aaggtcgacg tcctgatcgg
     3001 gtcggccggc gtgccttcgt cgatggcgat ggccgccgtc tcgcgcgagg ccaaggtgcc
     3061 gatgatcgcg ctgacgccga tcggcctggc cggccccgaa ggcgcctggg tcgtggcgat
     3121 cccgcagccg accggcctga tggtcgacgc ggtcatcgac cagatgaaga aggccggcgt
     3181 caagacggtc gcctacatcg gcttttccga cgcctggggc gacctcgtct acaacgcgct
     3241 gatcaagggc acagaaggca ccggcatcaa ggtcgtgacc aacgagcgct atgcacgccc
     3301 cgacacgtcg gtgagcgggc agatcctgaa gatcctcgcc acccatcccg acgcggtgat
     3361 gaccggcggc tccgggacgc ccggcgcgct gccgtacctc gagctcgcca agcgcggcta
     3421 caagggccgc atctacggca accacggcct catcaatccc gatttcgtcc gcgtcgccgg
     3481 cgact
//
LOCUS       DASRWS010000029         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2026457, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000029 DASRWS010000000
VERSION     DASRWS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026457"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VFF72_00495"
     CDS             <1..1977
                     /locus_tag="VFF72_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91659.1"
                     /translation="ERRASLADRARRRFGRHLAALAGLLFAGRAPTARAAAGASPASD
                     RPAPDDAPPAPIALPAGVEWETNDTDPLIGAPEALRGGSLRYPIDSYPLTFRLMGPNS
                     NDAFAGWSRLFTFNFTLVTRHPVTDRFIPLMATHWSVQKDQRTIYFKLDRDARFSDGH
                     PVTAADYVFTLEMMRSPHIVDPFYNTYAKQFYESVDRIDDYTLRIVGTRWSWRPMSDY
                     AGLWPTPAHATRLDADWVARSNNQFQIVPGPYVVSSAVRGESVTFERLAHWWGDGKRY
                     FIGQYNFDRIELRVIPTERSLDYLRRGEIDMITEGAARRWHEDYDFDAVNKGWLRRAR
                     IMVDFPSGISGLNMNLQAPIFANRDFRAAMQHLFDFDRLNQNLMFGEYFRLTSFFEGT
                     PYADKRLRPYPFSPAKAAALLARAGYRRPADLHATSFLGALGDVLRGLVFTRSDDDDV
                     LVNARGEKARFSVIYGSQSLERHLTVIQQDFRRAGVDMKLRLLEPGTAFERGLERKYE
                     MTLTNRTAGYYPDPRQYLGSEFKKATNNNDIWGYGSAEVDGLIRTYERDPDPEARLQA
                     MHRIDDIVHEEAFYIPFWTAPFIRVAWWDYVQFPKFYLPKRTEQLSDWLVYWIDPARR
                     AALAEAMRTGKAFAVDPEIDKDYWQVRQPPSPAS"
     gene            2011..3021
                     /locus_tag="VFF72_00500"
     CDS             2011..3021
                     /locus_tag="VFF72_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV91660.1"
                     /translation="MPLLEIDNLQVGFDTESGLLRAVDGVSLHVDAGQTLGVVGESGC
                     GKSVTASSILRLVPSPPGRFLGGAVRFDGVDILKMPRESLPSLRGREIAMVFQDPMTS
                     LNPVFTIGRQMGEVLSLRFGLSRAEARERSVATLQTVGLPDAAARLDAYPHELSGGMK
                     QRVMIAMALLCEPRLLIADEPTTALDVTVQAQILLLIRQLQKRTGAAVLFITHDMGVI
                     AEMCDAVVVMYAGRVVERAPVIDLFEHPRHPYTLGLLRSIPRRGVAHKAELPTIEGTV
                     PSLLSPPPGCRFAARCRRHQSLSEVERRRCLEDDPVLHAEGEGEVACHFPYTEAMSRE
                     LA"
BASE COUNT          492 a         1144 c         1043 g          515 t
ORIGIN      
        1 gagcgccggg cgtcactcgc cgaccgggcc aggcgacgct tcggcaggca tctcgcggcg
       61 ctggccggtt tgctgttcgc cggacgcgcg ccgacggccc gcgccgctgc cggcgcctcg
      121 cctgcgtccg atcgtcccgc acccgacgac gcgccgcccg cgcccatcgc actgccagcg
      181 ggcgtcgaat gggagaccaa cgacaccgac ccgctgatcg gtgctccgga ggcgttgcgc
      241 ggcggctcct tgcgctatcc gatcgattcg tacccgctga ccttcaggct gatggggccg
      301 aactcgaacg acgccttcgc cggctggagc cggctcttca ccttcaactt cacgctcgtg
      361 acgcgccatc ccgtcaccga ccgcttcatt ccgctgatgg ccacccactg gtcggtgcag
      421 aaagaccagc ggacgatcta tttcaagctc gaccgcgacg cccgcttcag cgacggccac
      481 ccggtcaccg ccgccgatta cgtgttcacc ttggagatga tgcgctcgcc gcacatcgtc
      541 gatcccttct acaacaccta cgccaagcag ttctacgagt cggtcgaccg catcgacgac
      601 tacacgctgc gcatcgtcgg cacccgctgg agctggcggc cgatgtccga ctacgcgggc
      661 ctgtggccga cgcccgcgca tgcgacgcgg ctcgatgccg actgggtggc ccgcagcaac
      721 aaccagttcc agatcgtgcc cgggccgtac gtcgtgtcca gcgcggtgcg cggcgagtcg
      781 gtgacgttcg agcgtctcgc gcattggtgg ggcgacggca agcgctactt catcggccag
      841 tacaacttcg accgcatcga gctgcgcgtg attccgaccg agcgcagcct cgactacctt
      901 cgccgcggcg agatcgacat gatcaccgag ggcgcggcgc gacgctggca cgaagactac
      961 gacttcgacg ccgtcaacaa gggctggctg cgccgggcgc ggatcatggt cgactttccg
     1021 tcgggcatct cgggcctcaa catgaacctg caggcgccga tcttcgccaa ccgcgacttc
     1081 cgcgcggcga tgcagcatct gttcgatttc gatcgcctga accagaacct gatgttcggc
     1141 gagtactttc gcctgacctc cttcttcgag ggaacgccct acgccgacaa gcggctccga
     1201 ccgtatccct tcagcccggc caaggcggca gccttgctgg cccgcgccgg ctaccgccgg
     1261 ccggccgatc tgcacgccac gagcttcctc ggcgccttgg gcgatgtgct gcgcggcctc
     1321 gtgttcacgc gctccgacga cgacgacgtg cttgtcaatg cgcgcggcga gaaggctcgc
     1381 ttcagcgtga tctacggctc gcagagcctg gaacggcacc tgaccgtgat ccagcaggat
     1441 ttccgtcgcg ccggcgtgga catgaagctg cgactgctcg agccgggcac cgccttcgag
     1501 cgcgggctgg agcgcaagta cgagatgacg ctgacgaacc gcaccgcggg gtactacccc
     1561 gatccccgcc agtacctcgg cagcgaattc aagaaggcaa cgaacaacaa cgacatctgg
     1621 ggctacggca gcgccgaggt cgacggcctg atccgcacct acgagcgcga tcccgatccc
     1681 gaggcgcggc tgcaggccat gcaccgcatc gacgacatcg tccacgagga ggccttctac
     1741 attccgttct ggacagcccc gttcatccgc gtcgcctggt gggactacgt gcagtttccc
     1801 aagttctacc tgcccaagcg caccgagcag ctgagcgact ggctcgtgta ctggatcgac
     1861 ccggcgcgcc gcgcggcgct ggccgaggcc atgcgcaccg gcaaggcctt tgcggtcgac
     1921 ccggagatcg acaaggacta ctggcaggtc cgccagcctc cttcgcccgc ttcctgagcc
     1981 gaaccgaaga agccgcccgg aaagcccgtc ttgccgctcc tcgagatcga caacctgcag
     2041 gtcggattcg ataccgaatc ggggctgctg cgcgcggtcg acggcgtctc gctgcacgtc
     2101 gatgccgggc agacgctggg cgtcgtcggc gaatcgggct gcggcaagag cgtcacggcg
     2161 tcgtcgatcc tgcgcctggt gccgagcccg ccgggacgct tcctgggcgg agccgtgcgc
     2221 ttcgacggcg tcgacatcct gaagatgccg cgtgagtcgc tgccgagcct gcgcggtcgc
     2281 gagatcgcga tggtcttcca ggacccgatg acttcgctca acccggtgtt caccatcggc
     2341 cgccagatgg gcgaggtgct gtcgctgcgc ttcggcctgt cccgcgccga ggcgcgcgaa
     2401 cgctcggtgg cgacgttgca gacggtcggg ctgcccgacg cggcggcgcg actcgatgcc
     2461 tatccgcacg agctctcggg cggcatgaag cagcgcgtga tgatcgccat ggcgctcctg
     2521 tgcgagccgc ggctgctgat cgccgacgag ccgaccaccg cgctcgacgt gacggtgcag
     2581 gcgcagatcc tgctgctgat ccgccagctg cagaagcgca ccggcgcggc cgtgctgttc
     2641 atcacccacg acatgggcgt gatcgccgag atgtgcgacg ccgtcgtcgt gatgtacgcc
     2701 ggccgtgtcg tcgaacgcgc tccggtgatc gacctcttcg agcatccccg ccatccctac
     2761 acgctgggcc tgctacggag cattccccgc cgcggcgtcg cgcacaaggc ggagttgccg
     2821 acgatcgagg gaacggtgcc gtcgctgctg tcgccgcccc cgggctgccg cttcgccgcg
     2881 cgctgccggc gccatcagtc gctttccgaa gtggagcggc gccgctgcct cgaagacgat
     2941 cccgtccttc atgcggaggg cgagggcgag gtcgcctgcc atttcccgta caccgaagcg
     3001 atgtcgcggg agctcgcgtg agcagcgcgc ccgacgcgcc gctgctggtc gtcgacgacc
     3061 tgcacaagca ttacccggtg cgcggcggcg tgctgggcgg caccatcgcc accgttcgcg
     3121 ccgtcgcggg ggtgagcttc agcgtgccgc gcggacagac gctcgggctg gtcggcgaat
     3181 cgggctgcgg caag
//
LOCUS       DASRWS010000030         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_304015, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000030 DASRWS010000000
VERSION     DASRWS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304015"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..178)
                     /locus_tag="VFF72_00505"
                     /pseudo
     CDS             complement(1..178)
                     /locus_tag="VFF72_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805899.1"
                     /note="catalyzes the formation of arginosuccinate from
                     citrulline and aspartate in arginine biosynthesis;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            137..1402
                     /gene="argG"
                     /locus_tag="VFF72_00510"
     CDS             137..1402
                     /gene="argG"
                     /locus_tag="VFF72_00510"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZV91661.1"
                     /translation="MAEIGGIGEDAGALPYAYTANLGQPDEPDYEDIPRRAMLYGAEK
                     ARLVDCRQQLAAEGIAALQCGAFHITTAGVTYFNTTPLGRAVTGTMLVQAMKEDGVSI
                     WGDGSTFKGNDIERFYRYGLLVNPQLRIYKPWLDQAFIDELGGRAEMSAFMTKAGFAY
                     KMSAEKAYSTDSNMLGATHEAKDLEHLASGIAIVQPIMGAAFWKDEVEVKRERVTVRF
                     DEGRPVALNGVEYVDRVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
                     LFIAYERLVTGIHNEDTIEQYREGGRRLGRLLYQGRWFDPQAIMLRETAERWVARAIT
                     GEVTIELRRGNDYSIMNTESPNLTYQPERLTMEKGESAFSPQDRIGQLTMRNLDIADT
                     RAKLATYAKVGLLSQQGGAPLPQIADGKK"
     gene            1577..1990
                     /locus_tag="VFF72_00515"
     CDS             1577..1990
                     /locus_tag="VFF72_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91662.1"
                     /translation="MAKDVADVLKTLTPLRAKLPKKVPPYLQKDWDVLLGAVDDTFAA
                     LQAAGGSVDTTKLRADYDALKKKLNALTTAAMTNIPKARAAQARMLTAANKIGAIVKD
                     KQNEKESKAVGQAIMAYAPYANREFSSLETPTLIP"
     gene            complement(2039..3121)
                     /gene="murB"
                     /locus_tag="VFF72_00520"
     CDS             complement(2039..3121)
                     /gene="murB"
                     /locus_tag="VFF72_00520"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZV91663.1"
                     /translation="MSLKPYNTFGLPAVAGTLVRIASDADVRRVVDDPVLGRAPKFIL
                     GGGSNIILTRDMPQIVLKVEVEGMRVVEERADAWIVEAGAGESWSELVEFTLDAGCPG
                     LENLALIPGTVGAGPVQNIGAYGVELKDRFESLDAVDLVTGRSVTLGPGICAFGYRDS
                     VFKQVPAEGLPPGAGVSAAGSGAPSADDGARHSLAGRCVITRVRFRLPRPWQPVLGYL
                     ELQRKMAETGVAAPAPRLIHDWIVGIRRAKLPDPAAIGNAGSFFKNPVVTPEQCRDII
                     GRDPEIVHYPMPDGSVKLAAAWMIDACVWKGKSVGQAGVYEKQALVLVNRGAAIGSEV
                     MTLARAIQESVYGRFGIRLEPEPVVV"
BASE COUNT          547 a         1095 c         1030 g          453 t
ORIGIN      
        1 agacgtggaa ggcgccggac tggatcgcgg cgatgccttc gtggaccaat tgcgtgcggc
       61 aatccaccag ccgggccttt tcggcgccga attcctcggc cttgcgcgga atctcgttgt
      121 agtcggcctc gtcaggctgg ccgagattgg cggtataggc gaagacgcgg gcgcccttcc
      181 ctacgcctac accgccaacc tcggccagcc cgacgagccc gactacgaag acatcccccg
      241 ccgcgccatg ctctacggcg ccgagaaggc gcgcctcgtc gactgtcgtc agcagctcgc
      301 ggccgaaggc atcgcggcac tgcagtgcgg tgccttccac atcacgactg ccggcgtcac
      361 ctacttcaac accacgccgc tcggtcgtgc cgtcaccggc accatgctcg tgcaggcgat
      421 gaaggaagac ggcgtctcga tctggggcga cggctcgacc ttcaagggca acgacatcga
      481 gcgcttctac cgctacgggc tgctcgtcaa tccgcaactg cgcatctaca agccctggct
      541 cgaccaggcc ttcatcgacg agctcggcgg ccgcgccgag atgtcggcgt tcatgacgaa
      601 ggccggcttc gcctacaaga tgagcgcgga gaaggcgtac tccaccgact cgaacatgct
      661 gggcgcgacg cacgaggcga aggacctcga gcacctggcg agcggcatcg ccatcgtcca
      721 gccgatcatg ggcgcggcct tctggaagga cgaggtcgag gtgaagcgcg agcgcgtcac
      781 ggttcgcttc gatgaaggcc ggccggtggc gctcaacggc gtcgagtacg tcgaccgggt
      841 cgagcttctc ctcgaggcca accgcatcgg cggccggcac ggcctgggca tgagcgacca
      901 gatcgagaac cgcatcatcg aggccaagag ccgcggcatc tacgaagcgc cgggaatggc
      961 gctcctcttc atcgcctacg agcggctcgt caccggcatc cacaacgagg acacgatcga
     1021 gcagtaccgc gagggcggcc gccgcctcgg ccggctcctc taccagggtc gctggttcga
     1081 cccgcaggcg atcatgctgc gcgagacggc cgagcgctgg gtggcgcgcg ccattaccgg
     1141 cgaggtgacg atcgagctgc gccgcggcaa cgactactcg atcatgaaca ccgagtcgcc
     1201 caacctcacc taccagcccg aacggctgac catggaaaag ggcgagtcgg cgttctcgcc
     1261 gcaggaccgg atcggccagc tgacgatgcg aaacctcgac atcgccgaca cgcgcgccaa
     1321 gctcgccacc tacgccaagg tcgggctgct ctcgcagcaa ggcggcgcgc cgctgccgca
     1381 gattgccgac ggcaagaagt agcccggcat cacgccggcc gatccggcag gaccgagccg
     1441 ctccgtgcgg atcgcgcggt ggggctctcg cgaagcgtat ttcgcgggcc gttcccgcca
     1501 cgcgttttcg gggggttcca caaagtccgg atcggcgcaa gagtcgtggt tccgactcat
     1561 cgcgaaggaa cacttcatgg ccaaggacgt agccgacgtt ctcaagaccc tcaccccgct
     1621 gcgcgcgaag ctgccgaaga aggtgccgcc ttacctgcag aaggactggg acgtcctcct
     1681 cggtgccgtc gacgatacct tcgccgcact gcaggcggcc gggggctcgg tcgacacgac
     1741 caagctgcgc gccgactacg acgcgctgaa gaagaagctc aacgccctca ccaccgccgc
     1801 gatgaccaac attccgaagg ctcgcgccgc ccaggccagg atgctgacgg ccgccaacaa
     1861 gatcggcgcc atcgtcaagg acaagcagaa cgaaaaggag tcgaaggccg tcggtcaggc
     1921 gatcatggcc tacgccccgt acgcgaaccg cgagttcagc agcctggaaa cgccgacgct
     1981 cattccctga ccgggccagg taccgcggcc gacctgccgc ggcatcgaca cgtcgcgctc
     2041 agacgaccac cggctcgggc tcgagccgga tgccgaagcg cccgtagaca ctttcctgga
     2101 tcgcccgggc cagtgtcatc acctccgagc cgatcgccgc gcctcgattg acgagcacca
     2161 gcgcctgctt ttcgtagaca ccggcctggc cgaccgactt ccccttccag acgcaggcgt
     2221 cgatcatcca ggcggcggcg agcttgacgc tgccgtcggg catcgggtag tggacgatct
     2281 cgggatcgcg gccgatgatg tcgcggcact gctcgggcgt gacgaccgga ttcttgaaga
     2341 agctgccggc gttgccgatc gcggccggat cgggcagctt ggcgcgccgg atgccgacga
     2401 tccagtcgtg gatcaggcgc ggcgccggcg cggcgacgcc cgtctcggcc atctttcttt
     2461 gcagctcgag gtagccgagc acgggctgcc acggccgcgg caggcggaat ctgacgcgcg
     2521 tgatgacgca gcggccggcc agcgagtgac gtgcgccatc gtccgctgac ggcgcgccgc
     2581 ttcctgccgc cgagacgccg gcccccggtg gcaagccctc ggccggcacc tgcttgaaga
     2641 cgctgtcgcg atagccgaag gcgcagatgc cgggccccag ggtgacgctt cggccggtga
     2701 cgaggtcgac cgcatcgagc gactcgaagc ggtccttcag ctcgacgccg taggcgccga
     2761 tgttctgcac cggcccggcg ccgaccgtgc ccggaatgag cgccaggttc tcgagacccg
     2821 gacagccggc gtcgagggtg aactcgacga gctcgctcca gctctcgccg gcgccggcct
     2881 cgacgatcca ggcatcggcg cgctcctcga cgacgcgcat gccctcgacc tcgaccttga
     2941 ggacgatctg cggcatgtcg cgcgtgagga tgatgttgct gccgccgccg aggatgaact
     3001 tgggcgcgcg ccccagcacc gggtcgtcga cgacgcggcg cacatcggcg tcgctggcaa
     3061 ttcgcacgag cgttcccgca accgccggca ggccgaaggt gttgtagggt ttgaggctca
     3121 cgccg
//
LOCUS       DASRWS010000031         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3753101, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000031 DASRWS010000000
VERSION     DASRWS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753101"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFF72_00525"
     CDS             complement(<1..303)
                     /locus_tag="VFF72_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91664.1"
                     /translation="MKPIRHIVVGAAAAIVALHAAAQVPAGYPANYAQTIADAKKEGK
                     VVIYSTTDTTSANPLIKDFEALYPGISVEYNDMNSTEIYNRFISEKAAGSGSADVLW"
     gene            complement(419..1522)
                     /gene="hemH"
                     /locus_tag="VFF72_00530"
     CDS             complement(419..1522)
                     /gene="hemH"
                     /locus_tag="VFF72_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZV91665.1"
                     /translation="MAFAPEPAFDHGNPARTAVLLCNLGTPAAPTTAAVRRYLAQFLS
                     DPRVVEIPRWVWWPLLHGLILRTRPQRSARRYASIWMPDGSPLAVWTRKQALLLEGYL
                     GERGQRVRVRHAMRYGDPSIASVLDALRADGVDRILVLPLYPQYAASTTASIGDDVSA
                     WQRRVRNVPEMRFVKHYHDDPGYIDALAARVDHHWMTHGRPDRLVLSFHGVPRRSLDL
                     GDPYHCECLKTGRLLAERLGLGEGMLMVTFQSRFGRAEWLKPYTEPTLVRLAGEGVAA
                     VDVMCPGFTSDCLETLEEIDQEARAAFLAAGGTTFGYIPCLNDRHEWIVALADIALRH
                     LQGWDTASPREDVGNPAVLEKRRQRAVAAGAPT"
     gene            complement(1537..2511)
                     /locus_tag="VFF72_00535"
     CDS             complement(1537..2511)
                     /locus_tag="VFF72_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZV91666.1"
                     /translation="MTQVSERTVLFADLRGSTSMYETLGNAEATTVVTEAVALLAGVV
                     AAHRGTVVKTLGDGLMAIFADSSSGIAAADDMHDSLARMRASGEISPEAVAQPMPLML
                     QVGLARGEVVEMSGDVFGDAVNVAARLLDHAGDNETLATHGVVENIGDWERSRFRSLD
                     RMQLRGRVEPVHVHLLEAVRRFGDTAATAFGDMAPAATEPEGIRLVWLDLNRIYAGTS
                     LPVILGRSPQATYIIDDNRVSRSHARIDWHGGTFQLVDLSYNGTYVRFDNDPEIISLR
                     RGACTLHGSGVIGLGTPPSEPISPCVRFEVMKFADTQRQAPFEFGLKS"
BASE COUNT          414 a          829 c          953 g          426 t
ORIGIN      
        1 ccagagcacg tcggccgaac cggagcctgc cgccttctcg ctgatgaagc ggttgtagat
       61 ctcggtcgag ttcatgtcgt tgtattcgac cgagatgccc ggatagagcg cctcgaaatc
      121 cttgatgagc gggttggcgg aggtcgtgtc ggtggtgctg tagatgacga ccttgccttc
      181 cttcttggcg tcggcgatgg tctgggcgta gttggccgga tagcctgcgg gcacctgtgc
      241 ggcggcgtgc agcgcgacga tcgccgcagc ggcgccgaca acgatatggc gaatgggttt
      301 catgggctct cgctgagaag ttcgttcgaa atgggtcggg ccttcgcggt gtcctggccc
      361 gggagaccgt gactatcgcg ctgcgggacc ccggccgcaa cagggcggac ccgggccttc
      421 aggttggcgc gccggccgcc acggcgcgct ggcgtcgctt ctcgagcact gccgggttgc
      481 cgacgtcttc gcggggcgat gccgtgtccc agccctggag gtgccgcagg gcaatgtcgg
      541 caagggcgac gatccattcg tgccggtcgt tcaggcaagg gatgtagccg aatgtcgtgc
      601 cgcccgccgc gagaaatgcg gcgcgggctt cctggtcgat ctcctcgagc gtctcgagac
      661 agtcggacgt gaatccggga cacatgacgt cgacggcggc gacgccttcg ccggccagcc
      721 gcaccagggt cggctcggtg tacggcttca gccattcggc ccggccgaac cggctctgga
      781 acgtcaccat gagcatgcct tcgcccaggc cgagccgttc cgcaaggagg cgaccggtct
      841 tcaggcactc gcagtgatac gggtcgccca ggtcgaggct gcgtctgggc acgccgtgaa
      901 agctgagcac cagccggtcg gggcggccgt gcgtcatcca gtgatggtcg acgcgcgcgg
      961 cgagggcgtc gatgtagccc gggtcgtcgt gatagtgctt gacgaagcgc atctcgggca
     1021 cgttgcggac gcggcgctgc catgccgaca cgtcgtcgcc gatgctcgcg gtcgtcgacg
     1081 cggcgtactg gggatagagc ggaaggacga ggatgcggtc aacgccatcg gcgcgcaagg
     1141 cgtcgagcac gctggcgatc gatggatcgc cgtagcgcat ggcatgacgc acgcgcaccc
     1201 gctgtccacg ttcaccgaga tagccctcga gaagcagcgc ctgctttcgc gtccagaccg
     1261 cgagaggcga gccgtcgggc atccagatgc tcgcgtagcg gcgcgccgat cgctgcggac
     1321 gggtccgcag gatgagcccg tgcaggagcg gccaccatac ccatcgcggt atctcgacga
     1381 cccgcggatc gctcaggaac tgggcgagat agcggcgaac cgcggccgtc gtcggcgccg
     1441 ccggtgtgcc cagattgcag aggagcacgg ccgtcctggc cggattgccg tggtcgaacg
     1501 ccggctcggg cgcgaaggcc atcgatcagc gcaccgtcag gacttgagcc cgaattcgaa
     1561 tggagcctgg cgctgcgtgt ccgcgaattt catcacctcg aatcgcacgc acgggctgat
     1621 cggctccgaa ggcggcgtgc ccaggccgat cacgccgctg ccgtgcaggg tgcaggcgcc
     1681 gcgccgcagg ctgatgatct cggggtcgtt gtcgaagcgg acgtaggtgc cgttgtagct
     1741 caggtcgacg agctggaagg tgccgccgtg ccagtcgatg cgggcgtgcg aacgcgatac
     1801 ccggttgtcg tcgatgatgt aggtggcctg cgggctgcga ccgaggatga ccggcaggct
     1861 ggtgccggcg tagatgcggt tgagatcgag ccagacgagg cggatgcctt cgggctcggt
     1921 ggcagccggc gccatgtcgc cgaaggcggt ggcggcggtg tcgccgaagc ggcggacggc
     1981 ttcgagcaga tgcacgtgaa ccggttcgac gcggccgcgc aactgcatcc ggtcgaggct
     2041 gcgaaagcgc gaccgttccc agtcgccgat gttctcgacc acgccatgcg tggccagcgt
     2101 ctcgttgtcg ccggcatggt cgagcagtct cgcggcgaca ttgacggcgt cgccgaacac
     2161 gtcgcccgac atctcgacga cttcgcctcg cgccagcccg acttgcagca tcagcggcat
     2221 cggctgggcg accgcctccg gagatatctc gccggacgcg cgcatccggg ccagcgagtc
     2281 gtgcatgtcg tcggcggcgg caatcccgga cgacgaatcg gcgaagatcg ccatcagccc
     2341 gtcacccagt gtcttgacga ccgtgccgcg gtgcgcggcg acgacgcccg cgaggagcgc
     2401 gaccgcctcc gtgacgacgg tggtcgcctc ggcgttgccg agcgtctcgt acatcgacgt
     2461 gctgccgcgc agatcggcaa agagaacggt gcgttcgctg acctgggtca tgccggaggg
     2521 aagggaaagc gcatcggctt gaagtttatc gcggcagggc acgacgaaag ggccgcgttg
     2581 cgacgcggcc ctttcggacg agcgaaccgc aatcgcgccg at
//
LOCUS       DASRWS010000032         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3180020, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000032 DASRWS010000000
VERSION     DASRWS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180020"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFF72_00540"
     CDS             <1..429
                     /locus_tag="VFF72_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZV91667.1"
                     /translation="TGLISNIFDSYEPFKGEIEGRKNGVLISQDDGEAVTYSLGKLDD
                     RGRMFVKAGDPLYEGMVIGIHSRDNDLVVNAVRGKQLTNFRVSGKEDAIKITPPIQLT
                     LEYAVEFIADDELVEITPKSIRIRKRHLKEHERKKASRES"
     gene            455..1831
                     /locus_tag="VFF72_00545"
     CDS             455..1831
                     /locus_tag="VFF72_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91668.1"
                     /translation="MSNAAGDDTRRPDDPALRQLEEALAADPKDAAAHRAMAHAMAER
                     GDELARLAHLIGAQTLDAFAAGAPAASTAELCKVATGYFMKGDVASAERWYRLVLLLD
                     PKVAAAHQNLAAIHADRGEFGEADECRRRAYAIQRVFVEPVEVPARRLLILCAGRTAG
                     NVPFETLLSSGHCDRIKYVIDFASEDEDRQLPPFDLVFNAIGEPDVAAPIAARLYRFV
                     ARCGRPVLNAPAAVVRTRRHRLVHLLGDLADVAVASCSHHERPPRSRAELTERLRQDE
                     MTLPVLARPEATHGGQGLVRCESLEALEQTLQQIDGPHYLTAFHDVRLEDGHYRKYRV
                     VFVDRKPHAYHLAISAHWMAHHFSADMAAQPWKIAEERRFLGDPAATLGDRAMAAIAA
                     IGRRLDLDYAGVDFTTLPDGRAFVFEANATMLVHFERANGPFAFRNAQVQRIVDAFGA
                     LLARRMVL"
     gene            complement(1972..>2479)
                     /locus_tag="VFF72_00550"
     CDS             complement(1972..>2479)
                     /locus_tag="VFF72_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZV91669.1"
                     /translation="YVGRSFSGDKEQLVPLIKGALRHRGAALLDVISPCVAFNNHAGS
                     TKSYDYVREHNEAVNRLDFMPRRDPIGAVYAPGEVIEVTQHDGSVLRLRKLAEGYDAG
                     DRRAAMSHIAAHEARGEILTGLLYVNAAAEDLHSHLHTVETPLNRLGADALCPGAKAL
                     EAINAEMV"
BASE COUNT          419 a          828 c          821 g          411 t
ORIGIN      
        1 accggcctca tcagcaacat cttcgacagc tacgagccgt tcaagggcga aatcgaaggc
       61 cgcaagaacg gcgtgctcat cagccaggac gatggcgagg ccgtgacgta ttcgctgggc
      121 aagctcgacg accggggccg catgttcgtg aaggcgggcg atccgctcta cgaaggcatg
      181 gtgatcggca tccacagccg cgacaacgat ctggtcgtga acgccgtgcg cggcaagcaa
      241 ctgacgaact ttcgcgtctc cggcaaggaa gacgcgatca agatcacgcc gccgatccag
      301 ttgacgctcg agtacgcggt ggaattcatc gccgatgacg agctggtgga gatcacgccg
      361 aagtcgatcc gcatccgcaa gcgtcacctg aaggaacacg agcgcaagaa ggcgtcgcgc
      421 gaaagctgaa atcgcggggc ctcggcgacg tcgcatgtcg aacgcagcag gcgatgacac
      481 ccgccggccc gacgatccgg cgctgaggca gctcgaagag gcgttggcgg ccgatccgaa
      541 ggatgcggcc gcgcatcggg cgatggccca cgccatggcg gagcgcggcg atgagttggc
      601 gaggctggcc catctgatcg gcgcacagac gctcgatgcg ttcgccgccg gcgcgccggc
      661 agcttcgacc gccgaactct gcaaggtggc gaccggctac ttcatgaagg gcgatgtcgc
      721 ctcggccgag cgttggtatc gcctcgttct gctgctcgat ccgaaggttg ccgccgcaca
      781 ccagaatctc gccgcaattc atgcagaccg cggcgaattc ggcgaggccg acgagtgtcg
      841 acgacgcgcg tatgcgatcc agcgcgtttt cgtcgagccc gtcgaggtgc ctgctcggcg
      901 actgctgatc ctgtgcgccg gacgaacggc cggcaacgtg ccattcgaga cgctgctttc
      961 gagcgggcac tgcgaccgga tcaaatacgt catcgatttc gcttcggaag acgaggacag
     1021 gcagctgcca cccttcgatc tcgtcttcaa cgccatcggc gagcccgacg tggcagcgcc
     1081 gatcgccgcc cggctgtatc gcttcgtggc ccgctgcggc cggcccgttc tcaacgcacc
     1141 cgctgccgtg gtccggacgc ggcggcaccg gttggtccat ctgctcggcg atcttgccga
     1201 cgtggctgtc gcgtcatgca gccaccatga gaggccacct cgatctcgcg ccgagttgac
     1261 cgaaaggttg cggcaggatg agatgacgct gcccgtgctg gctcgcccgg aagccacgca
     1321 tggcggccag ggactggtgc gctgcgaaag cctggaggcg ctcgagcaga cgctgcagca
     1381 gatcgacggc ccccactacc tcacggcctt ccatgacgtt cgcttggaag acggccacta
     1441 tcgcaagtac cgcgtcgtct tcgtcgatcg caagccgcat gcgtatcacc tcgccatctc
     1501 ggctcactgg atggctcacc atttctcggc cgacatggcg gctcagccct ggaagatcgc
     1561 agaggaacgc cggttcctcg gagatcccgc cgccaccctc ggcgaccggg ccatggctgc
     1621 gatcgccgcg atcggacgcc ggctcgacct cgactacgcg ggcgtcgact tcacgacgct
     1681 gcccgacggc cgggccttcg tgttcgaggc caacgccacc atgctcgtgc acttcgaacg
     1741 cgccaacggc ccgttcgcct tccgcaacgc gcaggtgcag cgcatcgtcg atgccttcgg
     1801 cgccttgctc gcccgcagga tggtcctctg acttcggact gatgcggcgt tgcaggtcgc
     1861 cagaccgtcg aatcctcgct tgccgcgaga agggcacgtc ccgaggcatc gagcatgccg
     1921 ggcagggggc tggccccctg aggcgagcgt cacctcggtg tgcggcaacc cctagaccat
     1981 ctcggcgttg atggcttcca gagccttggc acccgggcag agcgcatcgg ccccgagacg
     2041 gttcagtggc gtctcgacgg tgtgcaggtg cgagtgcagg tcttcggccg ccgcattgac
     2101 gtagagcagg ccggtgagaa tctcgccgcg ggcttcgtgg gcggcgatgt ggctcatcgc
     2161 ggcgcgccgg tccccggcgt cgtagccttc ggcgagcttt cgcaggcgaa ggacgctgcc
     2221 gtcatgctgc gtcacctcga tcacctcgcc tggcgcatag acagcgccga tcgggtcgcg
     2281 ccgcggcatg aagtcgagac ggttgaccgc ctcgttgtgc tcgcgcacat agtcgtagct
     2341 cttggtgctg ccggcgtggt tgttgaatgc gacgcacggg ctgatgacgt cgagcagcgc
     2401 cgcgccgcgg tggcgcagcg cccccttgat gagcggcacc agctgctcct tgtcacccga
     2461 gaagctgcga ccgacgtag
//
LOCUS       DASRWS010000033         4727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_12626, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000033 DASRWS010000000
VERSION     DASRWS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12626"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFF72_00555"
     CDS             complement(<1..654)
                     /locus_tag="VFF72_00555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV91670.1"
                     /translation="MTALAGPLEQRLEKLTLPLAVVLPGGQRIGSASAAVTVRLKELS
                     PLSHIVTGQVGKIGEDYVEGRIDIDGSMRDLMALGAELMPGDPVDASAVGPTPLGWVR
                     RLMHMARSRAHHQREADARQIQFHYDVSDDFYALWLDPKRVYSCAYFSDVGMSLEKAQ
                     EAKIEHICRKLMLKEGERFLDIGAGWGGLLLWAAEHYGVRARGITLSRNQHAYVNRLI
                     "
     gene            complement(701..1504)
                     /locus_tag="VFF72_00560"
     CDS             complement(701..1504)
                     /locus_tag="VFF72_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZV91671.1"
                     /translation="MSRVSSAAEAGCMANPMPVDRFNAADAARRTWPQLLESLLDAVC
                     LVEPEGLRIVAANAAAGELFGVEPCELIGRDMHAVASTPEDEVFWRDVAAGAAAGIVS
                     ESYVCRADGTPVPTLRRVSRVEPAPGTALYVVALRDRSTELRALRHLETSQAELQATL
                     ESISDGVLVTDLAGHIVNFNRRFAAIWRVPDEMLLLRADDEIAEWMLGQVSDPRAYSA
                     RLLDLETAEMMQSTDRIELRAGGCLERGSVPQCSRGRPVGRVFTFRVVR"
     gene            complement(1501..2808)
                     /locus_tag="VFF72_00565"
     CDS             complement(1501..2808)
                     /locus_tag="VFF72_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HZV91672.1"
                     /translation="MKAATHTHVIDVQALRVGMFVHLDVGWMSHPFPLSSFRITTPAQ
                     VATIRSLGLTQVRWSPQQSDPEALAPEPEKAAADVVEAAANESTIEPAADLDGSPSDR
                     AAAERGRRLQAQREAQQRCERQYGEASRACRQTLELVAGKPREAKAQAEALTRALVDK
                     MLVEQELCIRMLAENAGDKASMHAMNVGIVSLLMGRCFGFGAEDMLDLGVGAMMHDIG
                     KLDLPPRMRHREDNFSPSEVRVYEEHVELGLVQARRMGLSAGATAVIGQHHEHADGSG
                     FPGQLNTDRMTMPARIVALVNRYDSLCNPRLMAKAMTPHESLSLLFAQGRSKFDTSIL
                     GAFIKMMGVYPPGSAVQLTDDRYGLVVAVNSSRPLKPSVLVHEAGIAREDALVLDLET
                     TPGLGIRRSIRPQQLPPTALEYLAPGARVAYFFEPAAALEENA"
     gene            complement(2991..3482)
                     /locus_tag="VFF72_00570"
     CDS             complement(2991..3482)
                     /locus_tag="VFF72_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HZV91673.1"
                     /translation="MSSTSHGAVSGLAAPGLTFFADLTVEVGAPLEIGVVAGGRRRVI
                     PITGGRVVGRDWMARVVPGGADFQLVTSPTMARLEARYVLETDSGDLVYVHNDAVRTA
                     APEVMARLVRGEPVDANDVYFRCVPRFESAAPSLGWLAERLFLGTGVRRPDEVAMRFF
                     EVG"
     gene            3606..>4727
                     /gene="pobA"
                     /locus_tag="VFF72_00575"
     CDS             3606..>4727
                     /gene="pobA"
                     /locus_tag="VFF72_00575"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HZV91674.1"
                     /translation="MDIARTQVAIIGAGPAGLLLGALLAKAGIDNVILERRSADYVLG
                     RIRAGVLEQVTVDLLEAAGAAERLHAEGLPHDGIELCFGGKRHRIDLAGLTGGKRVTV
                     YGQTEVTRDLMALRAAAGLATIYEAEDVEPLGFEGRRPSVRYRHEDAVHELQCDFIAG
                     CDGYHGVSRHCVPASALRTFERVYPFGWLGVLSDTPPVSHELIYSNHERGFALCSMRS
                     TTRSRYYVQCSLDDKVEAWSDDAFWAELRTRLDPQAAATLVTGASIEKSIAPLRSFVA
                     EPMRFGRLFLAGDAAHIVPPTGAKGLNLATADVGLLARAFVEHFVAHSDAGIDSYSAR
                     CLRRVWRAERFSWWFTSLMHKFPETGAFGQKMQVAELDYL"
BASE COUNT          707 a         1678 c         1646 g          696 t
ORIGIN      
        1 gatcaggcgg ttgacgtagg cgtgctgatt gcggctgagc gtgatgccgc gggcgcgaac
       61 gccgtagtgc tcggccgccc acagcagcag gccgccccag cccgcgccga tgtcgaggaa
      121 ccgctcgccc tccttcagca tcagcttgcg gcagatgtgc tcgatcttcg cctcctgcgc
      181 cttctcgagc gacatgccga cgtcgctgaa ataggcgcag gaatagaccc gcttcggatc
      241 gagccagagc gcgtagaagt cgtcggagac gtcgtagtgg aactggatct ggcgcgcatc
      301 ggcctcgcgc tggtggtgcg cccgcgagcg cgccatgtgc atcagccggc gcacccagcc
      361 gagcggggtc ggaccgacgg cgctggcgtc gacgggatcg ccgggcatca gctcggcgcc
      421 gagcgccatc aggtcgcgca tgctgccgtc gatgtcgatg cgaccctcga cgtagtcttc
      481 gccgatcttg ccgacctggc cggtgacgat gtggctgagc gggctcagct ccttcaggcg
      541 caccgtgact gcggcgctcg ccgagccgat gcgctggccg cccggcaaca cgacggccag
      601 cggcagggtc agtttttcga gacgttgttc gagcggtccg gcgagcgcag tcatcggtgg
      661 aagttccttg cgaactcccg actttaccga cctcgaagga tcagcgcacg acgcggaagg
      721 tgaaaacccg gccgaccgga cggccacgac tgcactgcgg caccgatccg cgctcgaggc
      781 agccgccggc gcgcagctcg atccggtcgg tcgactgcat catctcggcg gtttcgagat
      841 cgagcaggcg tgccgagtag gcgcgcgggt cgctcacctg gccgagcatc cattcggcga
      901 tctcgtcgtc ggcgcgcagc agcagcattt cgtcgggcac gcgccagatc gccgcgaatc
      961 ggcggttgaa gttgacgatg tggccggcca ggtcggtcac caggacgccg tcgctgatcg
     1021 actccagcgt cgcctgcagc tcggcctgcg atgtctcgag gtggcgcagg gcgcgcaatt
     1081 ccgtgctccg gtcgcgcagg gccacgacgt agagcgcggt gcccggcgcc ggctcgacgc
     1141 ggctgacgcg gcgcagcgtc ggcaccggtg tcccgtcggc gcggcacacg tagctctcgg
     1201 agacgatgcc cgcggcggca ccggccgcca cgtcgcgcca gaagacttcg tcctcggggg
     1261 tcgaggccac cgcatgcatg tcgcgcccga tcagctcgca gggctcgacg ccgaagagct
     1321 ccccggccgc cgcattggcg gcgacgatgc gcaggccttc cggctcgacc aggcacaccg
     1381 cgtcgaggag cgactcgagc agttgcggcc aggtgcgccg ggccgcgtcg gcggcgttga
     1441 accggtcgac cggcatcgga ttggccatgc agcccgcctc ggcggcggac gacacgcggc
     1501 tcacgcgttc tcctccagcg ctgccgccgg ctcgaagaaa taggcgaccc gggcgccggg
     1561 cgcgaggtat tcgagcgccg tcggcggcag ctgctgcggc cggatgctgc gccggatccc
     1621 cagccccggc gtcgtctcga gatcgagcac cagcgcgtcc tcgcgagcga tgccggcttc
     1681 gtgcacgagc acgctcggct tgagcggacg agacgagttg acggcaacga cgaggccgta
     1741 ccgatcgtcg gtgagctgca ccgcggagcc cggcggatag acgcccatca tcttgatgaa
     1801 ggcgccgagg atcgaggtgt cgaacttgct gcgcccctgc gcgaagagca gcgacagcga
     1861 ctcgtgcggc gtcatcgcct tggccatgag gcgcggattg cacaggctgt cgtagcgatt
     1921 gacgagggcg acgatgcgcg ccggcatcgt catccggtcg gtgttgagct ggccgggaaa
     1981 gccgctgccg tcggcgtgct cgtggtgttg gccgatcacc gccgtcgcgc ccgccgacag
     2041 gcccatccgg cgcgcctgca cgaggcccag ctcgacgtgc tcctcgtaga cgcgaacctc
     2101 gctcggcgag aagttgtcct cgcgatgacg catccgcggc ggcaggtcga gcttgccgat
     2161 gtcgtgcatc atcgcgccga cccccagatc gagcatgtcc tcggcgccga agccgaagca
     2221 gcgccccatc agcagcgaga cgatgccgac gttcatcgca tgcatcgacg ccttgtcgcc
     2281 ggcgttctct gccagcatgc ggatgcacag ctcctgctcg accagcatct tgtcgaccag
     2341 cgcccgggtc agtgcctcgg cctgcgcctt cgcttcgcgc ggcttgccgg cgacgagctc
     2401 cagggtctgc cggcacgccc gcgaggcctc gccatactgt cgctcgcacc gttgctgcgc
     2461 ctcgcgctgc gcctgaaggc ggcggccccg ctcggccgcg gcgcgatccg aaggcgaccc
     2521 gtcgagatcg gcggccggct cgatcgtcga ttcgtttgcc gccgcctcga cgacatcggc
     2581 cgcggccttc tcgggttccg gcgcgagcgc ctcgggatcg ctttgctggg ggctccagcg
     2641 cacctgggtc aggccgagcg agcggatcgt cgcgacttgc gccggcgtcg tgatccggaa
     2701 gctgctgagc gggaacggat gcgacatcca gcccacgtcg agatgaacga acatcccgac
     2761 gcgaagcgcc tggacgtcga tcacgtgagt gtgggtggct gccttcatgt cgctcgttcc
     2821 gcatgccgac cggaggcatg agcggatagc gagttatcgg ccgtgccgtg gccgccttga
     2881 ggcgtcccgc gcagcgtgac ttccggcacg aacgcggcgc tcgacgcgtc cgggccatcc
     2941 gccgcggccc ggccgctgcg gccgcgacgg gatcgggcga aagagcgagc tcagccgacc
     3001 tcgaaaaaac gcatcgcgac ctcgtccggc cgccgcactc cggtccccag gaacagccgt
     3061 tccgcgagcc agccgagcga cggcgcggcc gattcgaagc gcggcacgca gcggaaatag
     3121 acgtcgttcg cgtcgacggg ctcgccgcga accagccgcg ccatgacctc gggcgcggcg
     3181 gtgcgaacgg catcgttgtg gacatagacg aggtcgcccg aatcggtctc gaggacgtag
     3241 cgcgcctcca ggcgtgccat cgtcgggctg gtgacgagct ggaaatcggc gccgccggga
     3301 acgacgcgcg ccatccagtc gcggccgacc acgcggccgc cggtgatggg gatgacgcgg
     3361 cggcgaccgc cggcgacgac gccgatctcg agcggcgcgc cgacctcgac cgtcaggtcg
     3421 gcgaaaaagg tcagcccggg ggcggcgagg cccgacacgg ccccatggga agtggacgac
     3481 atgcgaatct cctcggcagg gaagcagcac cgcgacgacg gggccgccac ggccaatggc
     3541 acagcgtacc gcttcgaccg acccggcgcg cacgccggtg cgccggcgcg gcacacttgc
     3601 gttccatgga catcgctcgc acccaggtcg ccatcatcgg cgccggcccc gccggcctgc
     3661 tgctcggggc cttgctcgcc aaggccggca tcgacaacgt catcctcgag cggcgcagcg
     3721 ccgactacgt gctcggtcgc atccgcgccg gcgtcctcga gcaggtgacg gtcgacctgc
     3781 tcgaggcggc cggtgccgcg gagcggctgc acgccgaagg attgccgcac gacgggatcg
     3841 agctttgctt cggcggcaag cgccatcgca tcgacctggc cggattgacc ggcggcaagc
     3901 gcgtcaccgt ctacgggcag accgaagtga cgcgcgacct catggcgctg cgcgccgccg
     3961 ccggcctggc cacgatctac gaagccgaag acgtcgagcc gctcggcttc gaaggccgcc
     4021 ggccctcggt gcgctatcgc cacgaggacg ccgtccacga gctgcagtgc gacttcatcg
     4081 ccggctgcga cggctaccac ggcgtcagcc ggcattgcgt gccggcctcg gcgctgcgca
     4141 cgttcgagcg cgtctacccc ttcggctggc tgggtgtcct ctcggacacg ccaccggtct
     4201 cgcacgagct catctactcc aaccacgagc gcggcttcgc gctgtgcagc atgcgctcga
     4261 cgacgcgcag ccgctactac gtgcagtgct cgctcgacga caaggtggag gcgtggagcg
     4321 acgatgcgtt ctgggccgag cttcgcacgc gcctcgatcc gcaggcggcg gccacgctgg
     4381 tcaccggcgc gtcgatcgag aagagcatcg cgccgctgcg cagcttcgtt gccgagccga
     4441 tgcgcttcgg acggctcttc ctggccggcg atgcggcaca catcgttccg cccaccggcg
     4501 ccaagggact caacctcgcc accgccgacg tcggtttgct ggcgcgcgcc ttcgtcgagc
     4561 acttcgtcgc gcacagcgac gccggcatcg acagctactc cgcgcgctgc ctgcggcgcg
     4621 tctggcgggc cgagcgcttc tcgtggtggt tcacgtcgct catgcacaag tttcccgaga
     4681 ccggcgcatt cggccagaag atgcaggtcg ccgagctcga ctacctc
//
LOCUS       DASRWS010000034         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2601833, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000034 DASRWS010000000
VERSION     DASRWS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601833"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /locus_tag="VFF72_00580"
     CDS             <1..2212
                     /locus_tag="VFF72_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV91675.1"
                     /translation="GEAQHFERVETSADGRRAMTWIHYVPDRHGEEVRGFFVLASDIS
                     ELKEAEGRLQRANAELVDARNRAEAATAAKSAFLANMSHEIRTPMNAIIGLTHLLLRD
                     LREPAQRDRMQKVSDAAHHLLEVINDILDLSKIESGKLQLDASDFALDAMLMRACSLV
                     TDAARAKSLEIVIDTDGMPRLLHGDATRISQALLNLLSNAVKFTARGAVTVRCSLLEQ
                     DESSLLARFAVHDTGIGIAADKIGVLFKAFEQADKSTTRRYGGTGLGLSITRELALLM
                     GGEAGVESELGVGSTFWFTVRLQAAREPMQPVRNALLAGTRGLVVDDLPEAREALVEM
                     LRRLGVRVDSAGSGEEALARVDAAEVASDPYRFCVLDWMMPGIDGIETCRRLRAGGRR
                     PGLRCILVTAHDEALVWTEARSAGIDNVLPKPVSASSLHDALGEALSREAPAAVAAAQ
                     PGEAFRILQSTRRGASVLLAEDNPVNQEVAVELLRSAGLCVDVADDGEQAVAMAGRGR
                     YDLVLMDVQMPGMDGLQATRALRRTPATSSTPVVAMTANAFGEDRDACIAAGMNDHVA
                     KPVDPDVFYNTLLRWLPSPESVTQEDANRAATPPLDTEAPAGDVDASSWPERLRSIDG
                     FDVDRGCDLFGGRWDLYRRVLATFASNYTRGLPDLRSAASPVSRSALAAAGHSLRGAS
                     GAIGAIRIQSLAGEIEALASRDGPPGELDDKAAALQSLLVDTAGRLRLILGLAA"
     gene            complement(2363..>2562)
                     /locus_tag="VFF72_00585"
     CDS             complement(2363..>2562)
                     /locus_tag="VFF72_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HZV91676.1"
                     /translation="LMKAIDAGGGKATLTTVEGGTLTATKSGHTIMLTDEKGGTADVT
                     IPDVVQSNGVIHVIDKVLLPK"
BASE COUNT          373 a          904 c          909 g          376 t
ORIGIN      
        1 cggcgaggcc cagcatttcg agcgcgtcga gacgagcgcc gacgggcgcc gggcgatgac
       61 ctggatccac tacgttcccg accgccatgg cgaggaggtg cgcggcttct tcgtcctggc
      121 ctcggacatc tccgagctga aggaggccga agggcgcctg cagcgggcca acgccgagct
      181 cgtcgacgcg cgcaatcgcg ccgaggccgc caccgccgcc aagagcgcgt ttctggccaa
      241 catgagccac gagatccgca cgccgatgaa cgcgatcatc ggcctgaccc atctgctgct
      301 gcgcgacctg cgggagccgg cgcagcgaga ccgcatgcag aaggtctccg acgcggcgca
      361 ccatctgctc gaggtgatca acgacatcct ggacctgtcg aagatcgaat ccggcaagct
      421 ccagctcgac gccagcgact tcgcgctcga cgcgatgctg atgcgggcgt gctcgctcgt
      481 caccgacgcg gcgcgtgcca agtcgctcga gatcgtgatc gacaccgacg gcatgccgag
      541 gttgctgcat ggcgacgcga cccggatctc gcaagcgctc ctcaacctgc tcagcaacgc
      601 cgtcaagttc acggcgcgcg gcgccgtcac cgtgcgctgc agccttctcg aacaggacga
      661 atcgtcgctg ctcgcgcgct tcgccgttca cgacaccggc atcggcatcg ccgccgacaa
      721 gatcggcgtg ctcttcaagg ccttcgagca agccgacaag tcgacgacgc gtcgctacgg
      781 cggcaccggc ctcggcctgt cgatcacgcg cgagctggcc ctgctgatgg gcggcgaggc
      841 cggcgtcgag agcgagctcg gtgtcggcag caccttctgg ttcacggtgc gcctgcaggc
      901 ggcccgcgag ccgatgcagc cggtccgcaa tgcgcttctc gccggcaccc gcggtctcgt
      961 cgtcgacgac ctgccggagg cccgcgaggc gctcgtcgag atgctgcgcc ggctcggcgt
     1021 tcgcgtcgac agcgccggct ccggcgagga ggcgctggcc cgggtcgatg cggcggaggt
     1081 ggcgagcgac ccgtaccggt tctgcgtgct cgactggatg atgccgggca tcgacggcat
     1141 cgagacgtgc cggcggctga gggcaggagg gcgccggccg ggcctgcgct gcatcctcgt
     1201 gaccgcgcac gacgaggcgc tggtctggac ggaggcgcga agcgccggca tcgacaacgt
     1261 ccttccgaag ccggtctcgg cgtcgagtct gcacgacgcg ctcggcgagg cgttgagccg
     1321 ggaagcgccc gccgccgttg ccgcagcgca gcccggcgag gcgttccgga tactgcaatc
     1381 gacgcggcgc ggcgcgagcg tgctgctcgc cgaggacaat cccgtcaacc aggaagtcgc
     1441 cgtcgagctg ttgcgctcgg ccgggctgtg cgtcgacgtc gccgacgacg gcgaacaggc
     1501 ggtggcgatg gcgggcaggg gccgctacga cctcgtgctg atggacgtgc agatgcccgg
     1561 catggacgga ttgcaggcga cccgtgcgct gcgccggacg ccggccacga gctcgacgcc
     1621 ggtcgtcgcg atgacggcga acgcgttcgg cgaggaccgc gatgcctgca tcgccgccgg
     1681 aatgaacgac catgtcgcca agcccgtcga ccccgacgtc ttctacaaca cgctgctgcg
     1741 ctggctgccc tcgcccgaaa gcgtgacgca ggaagacgcg aaccgcgccg cgacgccgcc
     1801 gctcgacacg gaggcgcctg ccggcgatgt cgacgcgtct tcctggccgg agcggcttcg
     1861 ctcgatcgac ggcttcgacg tcgaccgtgg ctgcgatctc ttcggcggcc gctgggattt
     1921 gtaccgtcgc gtgctggcga cctttgcgag caactacacc cggggcctgc ccgatctgcg
     1981 cagtgcggcc tcgcccgtct cacgatcggc gctggcggcg gccggtcact cgctgcgcgg
     2041 cgccagcggg gcgatcggcg cgatccggat ccagtcgctg gccggcgaga tcgaggcgct
     2101 cgccagccgc gacgggccgc cgggcgagct ggatgacaag gcggcggcgc tgcagtcgct
     2161 gctggtcgat acggccggcc gcctgcgcct catcctgggt cttgcggcct gagcggcgcg
     2221 ggctttgccg aaggaccggc cacttcgaac aggctgcgac cacgcgaagg ccaggcttcg
     2281 cccggtccct tcgggcaccg cgccgcgggc tgaaagcgcg aacggcgacc catgggccgc
     2341 cgtcgcgagc gaggcaggtc gactacttcg gcagcagcac cttgtcgatg acgtggatca
     2401 cgccgttcga ctggacgacg tcgggaatcg tgacgtcggc cgtgccgccc ttctcgtcgg
     2461 tcagcatgat ggtgtggccg cttttcgtcg cggtcagcgt gccgccttcg acggtcgtca
     2521 gcgtcgcctt gccgccaccc gcgtcgatcg ccttcatcag cg
//
LOCUS       DASRWS010000035         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_13013, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000035 DASRWS010000000
VERSION     DASRWS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13013"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFF72_00590"
     CDS             <1..412
                     /locus_tag="VFF72_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZV91677.1"
                     /translation="FRVSSAQIVFVVDEYGEVQGILTVRDVLEAITGEFRTETEADAW
                     AVQRADGSWLFDGLIPTPELKDRLDIKELPEEDRGRYNTLAGMVMLLLGRLPHTADAV
                     EWEGWRFEVVDLDGKRVDKVLASALVARPAETAS"
     gene            409..1182
                     /locus_tag="VFF72_00595"
     CDS             409..1182
                     /locus_tag="VFF72_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HZV91678.1"
                     /translation="MSRPPPSLYRRPVALDPAVHGAQRIAPLTDFSIARELHAVFVTA
                     TEFAQAALEFPIVFVETGRHDAAGQPRLSPLALLGLSHGENLHVEGPAWQARYIPASI
                     RRYPFSTAAMPGASGINVLVDEAWSGFSAQAGDPLFEPGGAPAPALKRAIDFLERFEH
                     ESERTQAFCQRIVSLGILKEMKADATLQDGQRLSVDGFHAVDEEKLRELPDSTLLELW
                     RTGLLMLIHAHMLSLANIRHLVNRKAARLEATRRADPVS"
     gene            1269..2228
                     /locus_tag="VFF72_00600"
     CDS             1269..2228
                     /locus_tag="VFF72_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV91679.1"
                     /translation="MSKAFASQADLADKKVTFTKLSENAYAYTAEGDPNTGIVVGDDA
                     VMVIDTQATPVMAADVIRHIRTVSDKPIKYVVMSHYHAVRVLGASAYAAEGASEIIAS
                     EDTRDLIVERGAADMKSEIERFPRLFRAVETIPGLTWPTITFKGEMTLWLGKLEVRLM
                     QLGRGHTKGDTVAWLPQQKILFSGDLVEYGATAYAGDAYFSDWPKTLDRVEALGATAI
                     VPGRGAALTDPKMCREGIATTRSFLRELFASVQAGVAKGHDLKRVYADTDAELRPKYG
                     QQFIYEHCMPFDVSRAFDEATAHRDPRIWTAERDKDMWAALQA"
     gene            2232..>4343
                     /locus_tag="VFF72_00605"
     CDS             2232..>4343
                     /locus_tag="VFF72_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZV91680.1"
                     /translation="MADRHDSSPPTDAAARFGSGRAVARVEDDALLRGAGRFTDNVAD
                     AGDAVIAFLRSPYPHARIVSVDADEARKLPGVLAVYTGADLAAAGVAPLPGSAGFARA
                     DGSPEVSPPRRALAHEVARYVGEPVAAVVATGRLAARDALEAIAVEYDDLPAVIGVAA
                     ATAAGAAALDPRAPDNVAAEMRHGSAADTDAAFAKAAHRIVLDLVNQRVAASPMEPRS
                     SVASFDRASGRFEVRISNQMPTAVAATIAAALPNVKQADVRVVVGDVGGGFGMKTGAY
                     PEDVAAAHAARELGRPVRWQADRSEEFLSANAGRDVASRAELALDASGRVLALRVRSH
                     ADIGAYATGAGVAIQLLIGPWVSTSIYDIATIDLHFLAVLTHTAPTGAYRGAGRPEAI
                     YLIERLMDAAARELRMDPAELRRRNMIRPAQMPYRNPMAQTYDSGEFEKILDEGLALA
                     DWGGFAARRAASEARGLLRGRGLAAFLEWTGGNVFEEKVSVDVTPDGFIEIFSATQQM
                     GQGIRTSYAQLAVDVFGVPIDRVRVVQGDTDRGNGFGSAGSRSLFVGGSAVRVAAERT
                     VEHAKTLAAQALEAAADDIEYRAGRFAVVGTDLGIDLFELAGKQAGARIHLDSTSSVG
                     GPTWPNACHVCEVEIEPATGEVQVVSYASVNDIGRVVSPTIARGQVDGGAVQGIGQAL
                     WERVVYDGESGQLLTGSYMDYL"
BASE COUNT          652 a         1569 c         1497 g          625 t
ORIGIN      
        1 gtttcgcgtc tcgagcgcgc agatcgtctt cgtcgtcgac gaatacggcg aagtgcaggg
       61 catcctcacc gtccgcgacg tgctggaggc gatcaccggc gagttccgca ccgagaccga
      121 ggccgatgcc tgggcggtgc agcgcgccga tggcagctgg ctcttcgacg gcctgatacc
      181 gaccccggag ctgaaggatc gcctcgacat caaggagctt cccgaggaag accggggccg
      241 ctacaacacg ctggccggca tggtgatgct gctgctcggc cgcctgccgc acaccgccga
      301 cgcggtcgaa tgggagggct ggcgcttcga ggtcgtcgat ctcgacggca agcgcgtcga
      361 caaggtgctg gcgagcgcgc tggtcgcgcg accggcggag accgcgtcgt gagccggccg
      421 ccgccctcgc tctaccgtcg tcccgtcgcg ctcgacccgg cggtgcatgg tgcgcagcgg
      481 atcgcgccgc tcaccgactt ctcgatcgcc cgcgagcttc atgcggtctt cgtcacggcc
      541 accgaattcg cgcaggcggc gctcgagttc ccgatcgtct tcgtcgagac cgggcgacac
      601 gacgccgccg gccagccgcg gctttcgccg ttggcgctcc tcggcctcag ccacggcgag
      661 aacctgcacg tcgagggccc ggcctggcag gcgcgctaca tcccggcgtc gatccgccgc
      721 tatccgttct cgacggcggc catgcccggt gcctccggca tcaatgtcct cgtcgacgag
      781 gcctggagcg gcttttcggc gcaggcgggc gatccactct tcgagcccgg cggcgccccg
      841 gcgccggccc tgaagcgggc catcgatttc ctcgagcgct tcgagcacga gagcgagcgc
      901 acgcaggctt tctgccagcg aatcgtctcg ctcggcatcc tcaaggaaat gaaggccgac
      961 gcgacgttgc aggacgggca gcgcctgtcg gtcgacggct ttcacgcggt cgacgaggaa
     1021 aagctgcgcg agctgccgga ctcgacgctc ctcgagctgt ggcgcaccgg gttgctgatg
     1081 ctgatccacg cgcacatgct gtcgttggcc aacatccgcc atctcgtcaa tcgcaaggcg
     1141 gcccggctcg aggcgacgcg gcgggcagac ccggtgtcgt agcgaacgct cgatcgaggt
     1201 ctgaggaagg ttttccgcgc atcgctcaga atcggcgcat tgccctcacg cgccccggcg
     1261 ccgacgccat gagcaaagca ttcgcatcgc aggccgacct cgccgacaag aaggtcacgt
     1321 tcacgaagct gtccgagaac gcctacgcct acacggcaga gggcgatccg aacaccggca
     1381 tcgtcgtcgg cgacgacgcg gtcatggtca tcgacacgca ggcgacgccg gtgatggcgg
     1441 ctgacgtcat ccggcacatc cgcacggtga gcgacaagcc gatcaagtac gtcgtgatga
     1501 gccactatca cgccgtccgg gtgctcggtg cgagcgccta tgcggccgag ggtgcgagcg
     1561 agatcatcgc cagcgaagac acgcgcgacc tgatcgtcga gcgcggcgcg gccgacatga
     1621 aaagcgagat cgagcgcttc ccgcgcctct ttcgcgccgt cgagacgatc cccggcctca
     1681 cctggccgac gatcaccttc aagggcgaga tgacgctctg gctcggcaag ctcgaggtca
     1741 ggctgatgca gctcggccgc ggccacacca agggcgacac cgtggcctgg ctgccgcagc
     1801 agaagatcct cttcagcggc gacctcgtcg agtacggcgc gacggcgtat gcgggcgacg
     1861 cgtatttctc cgactggccg aagacgctcg accgcgtcga ggcgctcggc gccaccgcca
     1921 tcgtccccgg ccgcggcgcc gcgctcaccg atccgaagat gtgccgcgaa ggcatcgcga
     1981 cgacgcgctc gttcctgcgc gagctgttcg cgagcgtgca ggccggcgtg gcgaagggtc
     2041 acgacctgaa gcgcgtctac gccgataccg atgccgagct gcgaccgaag tacggacagc
     2101 agttcattta cgagcactgc atgccgttcg acgtgtcgcg cgcgttcgac gaggcgacgg
     2161 cgcatcgcga cccgcgcatc tggacggccg agcgcgacaa ggacatgtgg gcggcgttgc
     2221 aggcctgagc gatggcagac cgtcacgatt cttccccgcc gaccgacgcc gcggcccgtt
     2281 tcggcagcgg tcgggcggtc gcccgcgtcg aggacgacgc gttgctgcgc ggcgccggcc
     2341 gcttcaccga caacgtcgcc gacgccggtg acgccgtcat cgcgttcctt cgctcgccgt
     2401 atccgcacgc ccgcatcgtc agcgtcgacg ccgacgaggc gcgcaagctg cccggcgtgc
     2461 tcgccgtcta taccggcgcc gatctggccg ccgccggcgt cgcgccgctg ccgggcagcg
     2521 ccgggttcgc ccgcgccgac ggcagcccgg aagtctcgcc gccgcgccgg gcgttggccc
     2581 acgaggtggc gcgctacgtc ggcgagccgg tggcggcggt cgtcgccacc ggtcgcctcg
     2641 ccgcgcgcga tgcgctcgag gcgatcgccg tcgagtacga cgaccttccc gccgtcatcg
     2701 gcgtcgccgc ggccaccgcc gccggcgccg cagcgctcga tccgcgcgcg cccgacaacg
     2761 tcgccgccga gatgcggcac ggcagcgccg ccgacaccga cgccgcgttt gcgaaagccg
     2821 cgcaccggat cgtcctcgat ctcgtcaacc agcgcgtcgc cgcgtcgccg atggagccgc
     2881 gctccagcgt cgcgtcgttc gacagggcgt ccggccgctt cgaggtgcgc atcagcaacc
     2941 agatgccgac ggcggtcgcg gcgacgatcg ccgcggcgct gccgaacgtc aagcaggccg
     3001 acgtgcgggt cgtcgtcggc gacgtcggcg gcggcttcgg catgaagacc ggcgcctatc
     3061 ccgaagatgt cgccgccgcc cacgcggcgc gcgagctcgg ccggccggtg cgctggcagg
     3121 ccgatcgcag cgaggagttc ctctcggcca acgccggacg cgacgtcgcc agccgcgccg
     3181 agctggccct cgacgcctcg ggtcgggtgc tggcgctgcg cgtccgctcg cacgccgaca
     3241 tcggcgccta cgccaccggc gccggcgtcg cgatccagtt gctgatcggc ccctgggtgt
     3301 cgaccagcat ctacgacatc gcgacgatcg acctgcactt tctcgccgtc ctcacgcata
     3361 ccgccccgac cggcgcgtat cggggcgcgg ggcgaccgga agcgatctac ctgatcgagc
     3421 ggctgatgga tgcggcggcg cgcgagctgc ggatggatcc ggccgaactg cgccggcgca
     3481 acatgatccg gcccgcgcag atgccttacc gcaatccgat ggcgcagacc tacgacagcg
     3541 gcgagttcga gaagatcctc gacgaaggac tggcgctcgc cgactggggc ggcttcgcgg
     3601 cgcggcgtgc ggccagcgag gcgcgtggcc tgttgcgcgg gcgcggcctc gccgccttcc
     3661 tcgaatggac cggcggcaac gtgttcgagg aaaaggtcag cgtcgacgtc actcccgacg
     3721 gcttcatcga gatcttctcg gcgacgcagc agatggggca gggcatccgc accagctacg
     3781 cgcagcttgc ggtcgacgtc ttcggcgtgc cgatcgaccg ggttcgcgtc gtccagggcg
     3841 acaccgaccg cggcaacggc ttcggcagcg ccggatcgcg ttcgctcttt gtcggcggat
     3901 cggcggtacg cgtcgccgcc gagcgcaccg tcgagcacgc gaagacgctc gccgcccagg
     3961 cgctcgaggc ggcggcggac gacatcgaat accgcgccgg gcgcttcgcc gtcgtcggca
     4021 ccgatctcgg catcgatctc ttcgagctcg ccggcaagca agccggcgcc cgcatccacc
     4081 tcgactcgac gagcagcgtc ggcggcccga cctggcccaa cgcgtgccac gtgtgcgagg
     4141 tcgagatcga gcccgcgacc ggcgaggtgc aggtcgtctc gtacgcttcg gtcaacgaca
     4201 tcggtcgtgt cgtcagcccg acgatcgccc gcggccaggt cgacggcggc gccgtgcaag
     4261 gcatcggcca ggcgctttgg gagcgcgtcg tctacgacgg cgaaagcggc cagcttctca
     4321 ccggcagcta catggactac ctg
//
LOCUS       DASRWS010000036         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3465004, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000036 DASRWS010000000
VERSION     DASRWS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465004"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFF72_00610"
     CDS             <1..229
                     /locus_tag="VFF72_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HZV91681.1"
                     /translation="IVTRSHDAAQRFLREVDSASVMVNASTRFADGFEYGLGAEIGIS
                     TDKLHARGPVGLEGLTSQKWIVIGQGEIRAQ"
     gene            219..923
                     /gene="queC"
                     /locus_tag="VFF72_00615"
     CDS             219..923
                     /gene="queC"
                     /locus_tag="VFF72_00615"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZV91682.1"
                     /translation="MPSDPRRALVLFSGGQDSTVCLAYALDRYAHVETIGFDYAQRHS
                     IELESRLHVRSQLHEKFPAWRGRMGDDHLLDLSLLAQISDTALTSQREIERNAGGLPN
                     TFVPVRNLLFFTFAASVADRRGLEVLVGGMCETDYSGYPDCRDNTLKALQVAISLGID
                     KPMTIDTPLMWLDKADTWAMTERLGGEPFIELVIEHSHSCYLGDREHRHAWGWGCGLC
                     PSCDLRRKGYDAWLAS"
     gene            1048..1575
                     /locus_tag="VFF72_00620"
     CDS             1048..1575
                     /locus_tag="VFF72_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZV91683.1"
                     /translation="MTSAQISVAAVVAVLAFWVVGAYNRLVGLRSALIERFAAVDTVL
                     RQRQRLFEKQIDLLAAALANAGPRVDALRAACRQADAARAHAQSRPGAAGAVTSLRVA
                     EEILADARARLPVQAIAGSDLPELNARIAEADAALAFARREFNAAVAAYNEAVRQSPT
                     ILVARLFGLRPAASF"
     gene            1602..2435
                     /locus_tag="VFF72_00625"
     CDS             1602..2435
                     /locus_tag="VFF72_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91684.1"
                     /translation="MSTDPHATHLATVDDEADAADRLSRLHGAVELRAGLLALILPAG
                     SKRAARAWEIETGSTPGVESLREHAAKLSGAARLPWFDVLLSRMALQPVAARKDLLQA
                     TRRVMAARGAARPIDRLHWLAMRRGLGEVAPLAARADGGVEVTEWLESDILALGAYTA
                     FLSRMIPGAPDEADPDAGARWCAAVMKTWQPFTDAPECTPPDTEGMVEALGKLQTMSW
                     MQRPVVIRGWVATALKHSGGARLGDLAADALRLTCVLLDSPQPPELARHYITLSHEGT
                     T"
     gene            2497..>3241
                     /locus_tag="VFF72_00630"
     CDS             2497..>3241
                     /locus_tag="VFF72_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZV91685.1"
                     /translation="MDRKDMTDAAPAAAATDSPAATPLPPVKAAAHDVGEPPQQERVL
                     LHMPVDIRSVALMVIAVLAGAFALQWGRAVFIPLLVGIMFSYALTPIVDTLETWHLPR
                     AAGAAAVLLALVGALGWGAWNLSDEASALVETLPDVAVKLRQAMQGQRQKPVATIEKV
                     QQAATEIERVAESATTPASAVPGEFAASSASAGAAASGASATAAPVPARRASRQARAA
                     SHPSPGGPSQTREVTHVVVEKPGFDIKGLL"
BASE COUNT          467 a         1158 c         1153 g          463 t
ORIGIN      
        1 gatcgtcacc cgctcgcacg acgcggcgca gcgcttcctg cgcgaggtcg actcggcgag
       61 cgtcatggtc aacgcatcga cgcgcttcgc cgacggcttc gagtacggcc tcggcgccga
      121 gatcggcatc tcgaccgaca agctgcatgc ccgcggcccg gtcgggctcg agggtctgac
      181 gtcgcagaag tggatcgtca tcggccaggg ggaaattcgt gcccagtgat ccacgacgcg
      241 ccctggtcct cttctccggc ggccaggatt cgaccgtctg cctggcctac gcgctcgacc
      301 ggtacgcgca tgtcgagacg atcggcttcg actatgcgca gcgccattcg atcgagctcg
      361 agagccgtct gcacgtgcgc tcgcagctgc acgagaagtt tccggcgtgg cgagggcgca
      421 tgggcgacga tcacctgctc gacctgtcgc tgctggcgca gatctccgat acggcattga
      481 ccagccagcg cgagatcgag cgcaatgccg gcggcctgcc gaacaccttc gtgccggtgc
      541 gcaacctgct cttcttcacc ttcgcggcga gcgtggccga ccggcgcggc ctcgaggtgc
      601 tggtgggcgg gatgtgcgag accgactact ccggctaccc cgactgccgc gacaacacgc
      661 tgaaggcgct gcaggtggcg atcagcctgg gcatcgacaa gccgatgacg atcgacacgc
      721 cgctgatgtg gctcgacaag gccgacacct gggcgatgac cgagcggctc ggcggcgagc
      781 ccttcatcga gctggtcatc gagcactcgc actcgtgcta cctgggcgat cgcgagcacc
      841 ggcatgcctg gggttggggc tgcggcctgt gtccgtcgtg cgacctgcgc cgcaagggct
      901 acgacgcctg gctcgccagc tgacgcggcg tcggcgcgca ggtgccgctt cggcgcgaag
      961 cgcgtcggcg cggcaaggtt cacgcccgcg acagcgtggc aaccagcggt cgcctggcga
     1021 caaggccgct cgccgtacat tgacgcgatg acgtccgccc agatctccgt cgccgccgtc
     1081 gtcgcggtgc tcgcattctg ggtcgtcggc gcctacaacc ggctggtcgg cctgcgcagc
     1141 gccctgatcg aacgctttgc cgcggtcgac accgtcctgc gccagcgcca gcgactgttc
     1201 gagaagcaga tcgacctgct ggcggcggcg ctggcgaacg ccggaccgcg cgtcgacgcc
     1261 cttcgcgcgg cgtgccgcca ggccgacgcc gcgcgcgccc atgcgcaaag ccgacccggc
     1321 gccgcgggtg ccgtgaccag cctgcgggtc gccgaggaga tcctcgccga cgcgcgcgcc
     1381 cgcctgccgg tgcaagccat cgccggctcc gacctgcccg agctcaacgc gcggatcgcc
     1441 gaggccgacg cggcgctggc gttcgcgcga cgcgagttca acgccgcggt tgccgcgtac
     1501 aacgaagccg tgcgacagtc gcccaccatt ctcgttgccc gcttgttcgg cctgcggccc
     1561 gccgcgtcgt tctagctcgg gctacatcca caagggttcg catgagcaca gatccgcacg
     1621 ccacgcacct agcgacggtc gacgacgaag ccgatgcggc cgatcgcctg tcgcggctgc
     1681 acggcgccgt cgagttgcgc gccgggctgc tcgcgctgat cctgcccgcc ggcagcaagc
     1741 gcgcggctcg cgcctgggag atcgaaacgg gctcgacgcc cggtgtcgaa agcctgcgcg
     1801 agcatgcggc caagctgtcg ggcgcggcgc gcctgccgtg gttcgacgtg ctgctgtcgc
     1861 gcatggcgct gcagccggtg gcggcacgaa aggatctgct gcaggcgaca cgccgggtca
     1921 tggccgcgcg cggcgcggcg cggccgatcg accggctgca ctggctggcg atgcgacgcg
     1981 ggctcggcga ggtcgcgccg ctcgccgcgc gggccgacgg cggggtcgag gtgaccgaat
     2041 ggctcgagtc cgacatcctg gcgctcggcg cctacacggc cttcctgtcg cgcatgattc
     2101 cgggtgcgcc ggacgaagcc gatcccgacg ccggtgcgcg ctggtgcgcg gcggtgatga
     2161 agacctggca gccgttcacc gacgcgcccg agtgcacgcc gcccgacacc gaaggcatgg
     2221 tcgaggcgct cggcaagctg cagacgatgt cgtggatgca gcgaccggtc gtcatccgcg
     2281 gctgggtggc gacggccctc aagcacagtg gcggcgcccg gctcggcgat ctcgcggccg
     2341 acgcgctgcg cctgacctgc gtcctgctcg actcgccgca gccgccggag ctggcgcggc
     2401 actacatcac gctcagtcac gaaggcacga cgtgaacggg cacgccgagt gccgtggacg
     2461 gcgcatcgac gggcatcgac gcaacgaagg gctttcgtgg atcgcaagga catgaccgac
     2521 gccgcgcctg ccgcggcagc caccgattcg cctgccgcca cgccgctgcc gccggtgaag
     2581 gcagccgccc acgatgtcgg cgagccgccg caacaggagc gcgtcctcct gcacatgccg
     2641 gtcgacattc ggagcgtcgc cctgatggtc atcgccgtcc tggccggcgc attcgcgctg
     2701 cagtgggggc gagccgtctt cattccgctc ctggtcggaa tcatgttcag ctacgcgctg
     2761 acgccgatcg tcgacacgct cgagacgtgg cacctgccgc gcgcggcggg agcggccgcg
     2821 gttcttctcg ccctcgtcgg cgcgctgggc tggggcgcgt ggaacctgag cgacgaagcc
     2881 agcgcactcg tcgaaacgct gcccgacgtc gccgtcaagc tgcgccaggc gatgcagggc
     2941 cagcgccaga agcctgtggc gacgatcgag aaggtgcagc aggcggccac cgagatcgaa
     3001 cgcgtggccg aaagcgcgac cacgccggcg tcggccgtgc cgggcgaatt cgcggcttcg
     3061 tcggcgtcgg ccggcgctgc ggcgagcggc gcttcggcaa cggctgcgcc ggtgccggcg
     3121 aggcgggcga gccgccaggc cagggcggcg tcgcatccgt cgcctggcgg cccgagccag
     3181 acacgcgaag tcacccatgt cgtggtcgaa aagcccggtt tcgacatcaa ggggctgctg
     3241 t
//
LOCUS       DASRWS010000037         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3753728, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000037 DASRWS010000000
VERSION     DASRWS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753728"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFF72_00635"
     CDS             complement(<1..879)
                     /locus_tag="VFF72_00635"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91686.1"
                     /translation="MKFDHADIQGNILSAYGKLGFPKGRFITLHIDIDKPELGRKFVS
                     ALLPSITTALRWPSKRARAKTGEVLAERPKVAVNIAFTFYGLIALGVPTRTLRGMPDE
                     FIDGMIERAPMLGDNFSGTSWLDSWDEVWRRAPQAARIVDPNTVHMLVTLNAQANPDG
                     TAVPELDAKTQEIEKLCETLGGVRVLPGHGRPSVRYQDLTAIMVKRDDGSIVTSPQEH
                     FGYIDALGDPVFDGQYPDHSENSRATGNGAFDGAGNWRPLATGEFLLGYPDEAQEIAG
                     ATMPLGFSRNGTFMAYR"
     gene            complement(892..1884)
                     /locus_tag="VFF72_00640"
     CDS             complement(892..1884)
                     /locus_tag="VFF72_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91687.1"
                     /translation="MPTYDGGHYFLTALVPIKTREIKDGAVVTSPLHALRKQLDLLPP
                     AAQTPACAGRQSPFAGSTRTHFVRFAVIDDVAYPGRLSGNTLVAAAAGDVLTKAQPQD
                     HLRSAFLLFVVDFDAASGDDSERDAYLSELWTVMGADLASIFQFCHGFDAADGKSFAA
                     YIARCQLETTMSFNDYYAVMPDLPSWPVNIYKWGAVASLAALGIGVLATAALLIASLL
                     GASVLPWLRDAVWLSLAGAAALILVALVAYMTVMAAGKKPFPAASDSDLKSVLKSLHV
                     QRAFTRFVIDNQVLAAGTDEPSAQKLHDAFKAFVAANKPYDLDGPTQAPGVIGF"
BASE COUNT          321 a          656 c          711 g          341 t
ORIGIN      
        1 gcggtacgcc atgaacgtgc cgttgcggct gaagccgagc ggcatggtgg cgccggcgat
       61 ctcctgcgcc tcgtcgggat agccgagaag gaactcgccg gtggcgagcg gccgccagtt
      121 gccggcgcca tcgaaggcgc cattgccggt cgccctgctg ttctcggagt gatcgggata
      181 ctgtccgtcg aacaccggat cgccgagcgc gtcgatgtat ccgaagtgct cctggggcga
      241 cgtgacgatc gagccgtcgt cgcgtttcac catgatcgcg gtcaggtcct ggtagcgcac
      301 gctcggccgg ccgtggcccg gcagcacgcg cacgccgccg agcgtctcgc acagcttctc
      361 gatctcctgc gtcttggcgt cgagctcggg cacggccgtg ccgtcggggt tcgcctgggc
      421 attcagcgtg accagcatgt gcaccgtgtt cgggtcgacg atgcgggccg cctgcggcgc
      481 cctgcgccac acctcgtccc agctatcgag ccagctcgtg ccgctgaaat tgtcgccgag
      541 catcggcgcg cgctcgatca tgccgtcgat gaactcgtcg ggcatgccgc gcagcgtgcg
      601 ggtcgggacg ccgagcgcga tcaggccgta gaacgtgaag gcgatgttga cggcgacctt
      661 gggccgttcg gccagcacct cgccggtctt ggcccgcgcc cgcttcgacg gccagcgcag
      721 cgcggtggtg atcgacggca gcaaggcgga gacgaacttg cgccccagct cgggcttgtc
      781 gatgtcgatg tgcaacgtga tgaaccgccc cttggggaag ccgagcttcc cgtaggcgct
      841 caggatgttg ccctggatat ccgcatgatc gaacttcatg gctgacgagc ctcagaatcc
      901 gatcacgccg ggggcctggg tcggcccgtc gagatcgtaa ggtttgttgg cggcgacgaa
      961 cgccttgaag gcgtcgtgga gcttctgtgc cgacggctcg tcggtgccgg cggcgagaac
     1021 ctggttgtcg atcacgaagc gtgtgaacgc ccgctggacg tgcagcgact tgagcacgct
     1081 cttgaggtcg gaatccgacg cggccggaaa cggcttcttg cccgccgcca tgacggtcat
     1141 gtaggccacc agcgcgacga ggatcagcgc ggcggcgccg gcgagcgaca gccacacggc
     1201 gtcccgcagc catggcagaa cggaggcgcc gagcagcgag gcgatcagca gcgccgcggt
     1261 cgccagcacg ccgatgccga gcgccgcgag cgaggcgacg gcgccccact tgtagatgtt
     1321 caccggccac gacggcaggt cgggcatcac cgcgtagtag tcgttgaacg acatcgtcgt
     1381 ctcgagctgg cacctggcga tgtaggccgc gaagctcttg ccgtcggcgg catcgaagcc
     1441 gtggcagaac tggaagatgc tcgccagatc ggcccccatc acggtccaca gctcggacag
     1501 gtatgcgtcg cgctcgctgt cgtcgccgct tgcggcgtcg aaatcgacca cgaacagcag
     1561 aaacgcggac ctcaggtgat cctgaggctg cgccttcgtc aggacgtcgc cggcggcggc
     1621 cgccaccagc gtgttgccgg acagtcgccc cggataggcg acgtcgtcga tgacggcgaa
     1681 gcgcacgaaa tgggttcgcg tgcttcccgc aaacgggctc tgccggccgg cgcacgccgg
     1741 cgtctgggcg gcgggcggca gcaggtcgag ctgcttgcgc aacgcgtgca gcggcgaggt
     1801 caccaccgcg ccatccttga tctcgcgcgt cttgatcggc acgagcgcgg tcaggaaata
     1861 gtgtccgccg tcgtatgtcg gcatcggtcg ctcctcgatc gaagtgctgg cttacaaagc
     1921 tgaccgtggg ctctgatcag gatcgttgag gtattgcgtg cgatggatgt cggcgttctc
     1981 ggtaacgcgg ctcgtcaccg gagcatagcc ctgatagccg aggtcgttc
//
LOCUS       DASRWS010000038         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2028039, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000038 DASRWS010000000
VERSION     DASRWS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028039"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFF72_00645"
     CDS             complement(<1..373)
                     /locus_tag="VFF72_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZV91688.1"
                     /translation="MRRSVATVSLSGTLRHKLEAVAAARFDAVELFENDFIAFNGSAR
                     ELRAIAGDLGLGIDLYQPFRDFEGMPDAAFGRSLERAERKFDLMQELGAPLMLVCSNT
                     SPLATGDVERSAAQLHALAERA"
     gene            complement(384..1217)
                     /locus_tag="VFF72_00650"
     CDS             complement(384..1217)
                     /locus_tag="VFF72_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZV91689.1"
                     /translation="MISGKTLLVAHLGFPTESFKAPMIYNPWFAARGIDAVVVPMAVE
                     AEDYPALLRTLANVGNVRGALVTMPHKVTTLALVDEATPTARIAGACNALLRRPDGSW
                     LGDQFDGAGFVRGLGHKGRRVEGSRVLVAGCGGVGSAIAASLAAAGVAQLDLFDSHPA
                     SAESLGQRLAGHYPGLAIRTGSNDPAGHDIVVNATPLGMKDDDPLPFDVERIDAGAFV
                     GEVVMKSEFTPLLRAAQARGLAVQVGTDMLFEMIPAYLEFFGFGTATADELRAVAQIR
                     Y"
     gene            complement(1474..1725)
                     /locus_tag="VFF72_00655"
     CDS             complement(1474..1725)
                     /locus_tag="VFF72_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91690.1"
                     /translation="MNTSFDQTTPQPSRWDQDGSEYVRSNIDWYARCSKRLVGLDPAL
                     TVEQALDLARDLSLDDALRARSPELVAEDLYRVELDLDD"
     gene            complement(1879..2640)
                     /locus_tag="VFF72_00660"
     CDS             complement(1879..2640)
                     /locus_tag="VFF72_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZV91691.1"
                     /translation="MVRAEGVHKRYGRDEVLKGIDLEVATGEVLCLIGPSGSGKSTFL
                     RCINHLEKIDAGRIEVDGVLVGYREKAGRLYEMGEREVCRQRAAIGMVFQRFNLFPHM
                     TALENVIEAPIRVRGEARAAAVGRARDLLDRVGLAAKADAYPSRLSGGQQQRVAIARA
                     LAMQPRLMLFDEPTSALDPELVDEVLAVMRNLADEGMTMIVVTHEMAFARDAGDRVVF
                     MDGGVVVEAGPPAEVLGRPQHERTKAFLSRVTSAA"
     gene            complement(2685..>3063)
                     /locus_tag="VFF72_00665"
     CDS             complement(2685..>3063)
                     /locus_tag="VFF72_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV91692.1"
                     /translation="GAYMAEIVRAGILAVDHGQVDASMALGMSRGLAMRRIILPQAMR
                     VIMPPAGNQFISLLKATSLVSVIAGGDLLTAAQNISSANLKTIELMLVATFWYLVLTT
                     ITSIGQYFVERRLARAGTGLRAR"
BASE COUNT          449 a         1034 c         1142 g          438 t
ORIGIN      
        1 cggcgcgctc ggcgagggcg tgcagctgcg ccgcggaacg ttcgacgtcg cccgtggcca
       61 gcggcgaggt gttcgagcag accagcatga gcggcgcgcc gagctcctgc atgaggtcga
      121 acttgcgctc ggcgcgctcc aggctccggc cgaaggcggc gtcgggcatg ccttcgaagt
      181 cgcgaaacgg ctggtacagg tcgatgccga gcccgagatc gccggcgatc gcgcgcagct
      241 cgcgggcgct gccgttgaag gcgatgaagt cgttctcgaa cagctcgacc gcatcgaagc
      301 gcgccgcggc gacggcctcg agcttgtggc gcaaggtgcc gctcagcgag acggtggcga
      361 ccgatcgacg catgcgaggc ttctcagtat cggatctgcg cgaccgcgcg cagctcgtcg
      421 gcggtggccg tgccgaagcc gaagaactcg aggtaggccg ggatcatctc gaagagcatg
      481 tcggtgccga cctgcaccgc cagcccgcgc gcctgcgcgg cgcgcagcag cggcgtgaac
      541 tccgacttca tcacgacctc gccgacgaag gcgccggcgt cgatgcgctc gacgtcgaag
      601 ggcagcggat cgtcgtcctt catgccgagc ggcgtcgcgt tgacgacgat gtcgtggccc
      661 gccgggtcgt tcgatccggt acggatcgca aggcccgggt agtgcccggc gagtcgctgc
      721 ccgagcgatt cggccgacgc cggatgcgag tcgaacaggt cgagctgcgc gacgccggcg
      781 gccgccagcg acgcggcgat cgccgagccg acgccgccgc agccggcgac gaggacgcgg
      841 ctgccttcga cgcggcgacc cttgtgcccg agcccgcgaa cgaagccggc accgtcgaac
      901 tggtcgccga gccagcttcc gtcgggccgc ctgagcaggg cgttgcaggc accggcgatc
      961 cgggccgtcg gtgtcgcctc gtcgaccagg gcgagcgtcg tcaccttgtg cggcatcgtc
     1021 acgagggcgc cgcggacgtt gccgacgttc gccagggtgc gaaggagcgc cggatagtct
     1081 tcggcctcga cggccatcgg cacgacgacg gcgtcgatgc cgcgcgccgc gaaccagggg
     1141 ttgtagatca tcggcgcctt gaacgactcg gtcggaaaac cgagatgggc gacgagcagc
     1201 gtcttgccgc tgatcatgcg cgcgtctccg gcgtgcccgg cgtgcccggc gcggcccgcc
     1261 gcagcccggc gactggtgcg aggcgcgact ttctgctctg gcgatccatg cgcgcatcgt
     1321 acgttctcgc acgcgcggtc ggcggtgagc ggcgaggcga tgccggcggt gccgacaggg
     1381 gatgcgccgc ggcgctgccg gcggtgccga cgggggacga gccgcggcgc tgacggcggc
     1441 gacaggtcga ggggctgcct ttcggcgcgc ggctcagtcg tcgaggtcga gctcgacgcg
     1501 atagaggtct tcggcgacga gctcgggcga gcgcgcgcgc agcgcgtcgt cgaggctcag
     1561 gtcgcgcgcc aggtcgagcg cctgctcgac ggtgagagcg gggtcgaggc ccaccaggcg
     1621 cttcgagcag cgcgcgtacc agtcgatgtt cgagcgcacg tactcggagc cgtcctggtc
     1681 ccagcgcgag ggctgcggcg tggtctggtc gaacgaggta ttcatgatcg tgcgttgcgt
     1741 cgtgaggatc gaagcccaaa gtctggcccg cctcccggcg catgcacagg ccgggcgccg
     1801 ccgcccgtcc agagacgctc gtaaccgcca gtcaattcgt gaagttttct tcgcgaccgc
     1861 gaatggccga cgagcgcgct aggcagcgct ggtgacgcgc gagaggaacg ccttcgtgcg
     1921 ctcgtgctgc ggccgtccga gcacctcggc cggcgggccg gcctcgacga cgacgccgcc
     1981 gtccatgaag acgacgcggt cgccggcgtc gcgcgcgaac gccatctcgt gggtgacgac
     2041 gatcatcgtc atgccctcgt cggcgaggtt gcgcatcacc gccagcacct cgtcgacgag
     2101 ctcggggtcg agcgccgatg tcggctcgtc gaagagcatg agccgcggct gcatggccag
     2161 ggcgcgggcg atcgcgactc gctgctgctg gccgcccgag aggcggctgg ggtaggcgtc
     2221 ggccttcgcg gcgaggccga cgcgatcgag caggtcccgc gcccgcccga ccgccgccgc
     2281 gcgtgcttcg ccgcgcacgc ggatcggcgc ctcgatgacg ttctcgagcg cggtcatgtg
     2341 cggaaagaga ttgaagcgct ggaagaccat gccgatggcc gcgcgctgtc ggcagacctc
     2401 gcgctcgccc atctcgtaga ggcggccggc cttctcgcgg tagccgacca agacgccgtc
     2461 gacctcgatg cggccggcgt cgatcttctc gagatgattg atgcagcgaa gaaacgtgct
     2521 ctttcccgaa cccgaggggc cgatcaggca gaggacctcg ccggtcgcga cctcgaggtc
     2581 gatgcccttg agcacctcgt cgcggccgta tcgcttgtgg acgccttcgg cgcgcaccat
     2641 cggcgcgccg gcgccgcggt cggggagcgt cggcgcggtg tccatcagcg agcccgcagt
     2701 cccgtgccgg cgcgggccag ccgacgctcg acgaaatact ggccgatgct cgtgatcgtc
     2761 gtcagcacga ggtaccagaa ggtggcgacg agcatcagct cgatcgtctt caggttggcc
     2821 gacgagatgt tctgggcggc ggtgagcagg tcgccgccgg cgatcaccga gaccagcgag
     2881 gtcgccttga gcagcgagat gaactggttg ccggccggcg gcatgatgac gcgcatggcc
     2941 tgcggcagga tgatccgccg catcgcgagg ccgcgcgaca tgccgagcgc catcgacgcg
     3001 tcgacctggc catggtcgac ggcgaggatg ccggcgcgga cgatctcggc catgtaggcg
     3061 ccc
//
LOCUS       DASRWS010000039         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1742711, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000039 DASRWS010000000
VERSION     DASRWS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742711"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFF72_00670"
     CDS             <1..426
                     /locus_tag="VFF72_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZV91693.1"
                     /translation="GGYMVAWMNGHVKADRYRAYVCHAGCFDWRGMFAGDAWRWHSKE
                     LGATYWVDPAKVQAQSPDAFVAGMKTPTLVVHGALDYRVPDAQGLAYYNTLKELGVDA
                     RLVWFPDENHWILKPRNSKLWYAELFAWLRTHDPVARKS"
     gene            423..734
                     /locus_tag="VFF72_00675"
     CDS             423..734
                     /locus_tag="VFF72_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91694.1"
                     /translation="MNDDLPYLRQPSLRGEQIVFVADDDLWSVAASGGAARRLTAGLS
                     EPATPCLSPDGRRLAFVSRDEQHPEVYVMAAEGGVATRLTWLGADTLVRGGRPLARSC
                     S"
     gene            complement(766..1050)
                     /locus_tag="VFF72_00680"
     CDS             complement(766..1050)
                     /locus_tag="VFF72_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91695.1"
                     /translation="MPTPSPSPRNQILSPSHIGLVRFPASSLMRLNVPDPSASIQSAP
                     AVPPRYRFQRAGSAVLRPITHRSPGPKARWLTWPQGRARGTPPSAAIVKA"
     gene            1344..>3323
                     /locus_tag="VFF72_00685"
     CDS             1344..>3323
                     /locus_tag="VFF72_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZV91696.1"
                     /translation="MALWEGAARRFASEGRVRQGQWLADGRSLVAVDDASGEERVVVF
                     DGGAPRPLPWDVGHVTRLRAAPQGTLVAIANHRNEVLVGDVASGALTTIDASDFGRSE
                     HLAWSPDAAWLAYSAATSTRHVAIKLHEVAARRNTLVTRPEFRDYSPSFDPDGKYLYF
                     LSLRTYDPVYDSVQFELSFPRAARPYLIALQAGGQPPFDPQPRGLAPDAAGAKGERTA
                     ANAAPALRIDLDGIDARVAAFPVSEGRFERIAGAAGGKVVWNMQNVVGAHGRGGHKEV
                     AGKLELFEFATLRTETLADKADAFEIAADGVSLVYRDGKRLRAIAAEKPPGASPENAI
                     DDKTPSRRNGWLDLERLRVSVEPRREWRQMFREVWRLQRDQFWAADMSGVDWEAAYRL
                     YAPLVERAATRSDLSDLIWEMQGELGTSHAYEMGGDHRKPPTMALGHLGCELRFDAAD
                     DSYEIVRIVRGDPWDAGADSPLNAVGVEAIVGERIVAVGGQRVSHETTPSSLLVHQAG
                     CKVALTLRRHAGADAATREVTVTALADEVPARYREWVEKNRAWVHERSKGRVGYFHLP
                     DMMAAGFAEFHRYFGNECARDALVVDLRYNRGGHVSELLLEKVARRRIAYNFPRWGKP
                     VSYPEEAAAGPVVALTNEQAGSDGDIFSHGFKLMKI"
BASE COUNT          498 a         1201 c         1134 g          490 t
ORIGIN      
        1 ggcggctaca tggtggcctg gatgaacggc cacgtgaagg ccgaccgcta tcgcgcctat
       61 gtctgccacg ccggctgctt cgactggcgc ggcatgttcg caggcgacgc ctggcgctgg
      121 cactcgaagg agctcggcgc gacctactgg gtcgaccccg cgaaggtgca ggcgcaaagc
      181 cccgacgcct tcgtcgcagg gatgaagacg ccgaccctgg tcgtccacgg cgccctcgac
      241 tatcgcgttc ccgacgcgca aggcctggcg tactacaaca ccctcaagga gctcggcgtc
      301 gacgcgcgcc tcgtctggtt tccggacgag aaccactgga tcctcaagcc gcgcaattcg
      361 aagctctggt atgccgagct gttcgcctgg ttgcgcaccc acgaccccgt cgcccgaaaa
      421 tcatgaacga cgacctgccc tatctccgcc agccgagcct ccggggcgag cagatcgtgt
      481 tcgtcgccga cgacgatctc tggtcggtcg cggcgagcgg cggtgcggcg cgccggctga
      541 ccgccgggct ctcggagccg gcgacgccct gcctgtctcc cgatggccgc aggctggcct
      601 tcgtgagtcg cgacgagcag catccggagg tctatgtcat ggccgccgaa ggcggcgtcg
      661 ccacgcgcct cacctggctc ggcgccgaca cgctggtgcg cggtggacgc ccgctggcga
      721 gatcgtgttc gtgacgacac aggcccagcc cttctttcgc aatcatcacg ccttcacgat
      781 cgccgccgag ggcggcgtgc cgcgcgccct tccctggggc caggtcaacc acctcgcctt
      841 cggccccggc gagcgatgcg tgatcggccg caacaccgcc gatccggcgc gctggaagcg
      901 ataccggggc ggtaccgccg gcgcgctctg gatcgacgcc gagggatcgg gcacgttcag
      961 gcgcatgagc gagctggccg gaaacctcac cagcccgatg tggctgggcg aaaggatctg
     1021 gtttctcggc gatggcgaag gcgtcggcaa cctctactcc tgccgacccg acggcagcga
     1081 gctgcgccgc cataccgatc acgacgactt ctacgcccgc catgcgcaga ccgatggccg
     1141 tcgcatcgtc tatcagtgcg gcggcacgat ctggctgttc gatccggcca ccgaccgggc
     1201 gcaaccggtc gacatccgca cgccctcgca tcgcagccag tcggctcgca agttcgtcgc
     1261 cgcggccgag gcacctggaa tcgtttcacc cgcacccggc gggccattcg ctggcgctgg
     1321 tcgcgcgcgg ccagttgttc ggcatggcgc tgtgggaagg cgcggcgcgg cgcttcgcga
     1381 gcgaggggcg cgttcgccag ggccagtggc tcgccgacgg ccgaagcctc gtcgctgtcg
     1441 acgatgcgtc cggcgaggag cgcgtcgtcg tgttcgacgg cggcgcgccg cgtccgctgc
     1501 catgggacgt cggccacgtc acgcggcttc gcgccgcgcc gcagggaacg ctcgtcgcga
     1561 tcgccaacca ccgcaacgag gtcctcgtcg gcgatgtcgc cagcggcgcc ctgacgacga
     1621 tcgacgccag cgacttcggt cgcagcgagc acctcgcctg gtcgcccgat gccgcctggc
     1681 tcgcctacag cgccgcgacc agcacgcgtc acgtcgcgat caagctgcac gaggtcgccg
     1741 cccgcaggaa cacgctcgtc acccgacccg agttccgcga ctattcgccc tcgttcgatc
     1801 ccgacggcaa gtacctctac ttcctgtcgc ttcgcaccta cgacccggtc tacgacagcg
     1861 tccagttcga gctgagcttt ccgcgcgcgg cacggccgta cctgatcgcg ctgcaggccg
     1921 gtggccagcc accgttcgac ccgcagccgc ggggactggc gcccgacgcg gcgggcgcga
     1981 agggcgagcg aacggcagcg aacgcagcgc ccgcgttgcg gatcgatctc gacggcatcg
     2041 acgcccgcgt cgccgcgttc cccgtcagcg agggtcgctt cgagcgcatc gccggtgctg
     2101 ccggcggcaa ggtcgtctgg aacatgcaga acgtcgtcgg cgcgcacggc cgcggcggtc
     2161 acaaggaggt ggcgggcaag ctggagctgt tcgaattcgc caccctgcgc accgagacgc
     2221 tggccgacaa ggccgacgcg ttcgagatcg cggccgacgg cgtctcgctg gtctaccgcg
     2281 acggcaagcg gctgcgcgcc atcgccgccg agaagccgcc tggcgccagc cctgagaacg
     2341 ccatcgacga caagacgccg tcgcgccgga acggctggct cgatctcgag cggcttcgcg
     2401 tctcggtcga gccgcggcgc gaatggcggc agatgtttcg cgaggtctgg cgcctgcagc
     2461 gcgaccagtt ctgggcggcc gacatgtcgg gcgtcgactg ggaagccgcc taccgcctct
     2521 acgcgccgct ggtcgagcgc gccgcgaccc gctccgacct gtccgacctg atctgggaga
     2581 tgcagggcga gctcggcacg tcgcacgcct acgagatggg cggcgaccat cgcaagcctc
     2641 cgacgatggc gctcggccat ctcggctgcg agcttcgctt cgacgccgcc gacgacagct
     2701 acgagatcgt gcggatcgtt cgcggcgatc cgtgggacgc cggcgccgat tcaccgctca
     2761 acgcggtcgg cgtcgaggcg atcgtcggcg agcggatcgt cgccgtcggc gggcaacgcg
     2821 tgtcgcacga gacgacgccc tcgtcgttgc tggtgcatca ggccggatgc aaggtcgcgc
     2881 tcacgctcag gcggcacgcc ggcgccgacg cggcgacgcg cgaggtcacc gtgacggcgc
     2941 tcgccgacga ggtgccggcg cgctatcgag aatgggtcga gaagaaccgc gcctgggttc
     3001 acgagcgatc gaaaggccgc gtcggctact ttcacctgcc cgacatgatg gccgccggct
     3061 tcgccgagtt ccaccgctat ttcggcaacg aatgcgcccg cgacgcgctg gtggtcgacc
     3121 tgcgctacaa ccgcggcggc cacgtctccg agctgctgct cgagaaggtc gcccggcgcc
     3181 gcatcgccta caactttccg cgctggggca agccggtcag ctatcccgaa gaggccgcgg
     3241 ccggcccggt cgtggcgctg accaacgagc aggcgggctc ggacggcgac atcttctcgc
     3301 acggtttcaa gctgatgaag atc
//
LOCUS       DASRWS010000040         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1167927, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000040 DASRWS010000000
VERSION     DASRWS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167927"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFF72_00690"
     CDS             complement(<1..738)
                     /locus_tag="VFF72_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZV91697.1"
                     /translation="MKRRLALAAFAAPALGVLPGLSLAQARPIHFIVPYPAGGPLDIM
                     ARALAEKVKDSLGPIVVENKPGAGGNIGADFVAKAAPDGLTLVMGAVATHAINPWLYA
                     RLPYDPIRDFTPITGVARVPNVLVMTPETASRLGIASVVDLVDYAKKNPGKLNYGSGG
                     NGSAGHLAGEMFKAEAGVHMVHIPYAGGNPAQLALLSGQVDLTFDNLASAAANIKAGR
                     LKALAVTTARRSSAMPDVPTIAEAGRSV"
     gene            complement(735..1748)
                     /locus_tag="VFF72_00695"
     CDS             complement(735..1748)
                     /locus_tag="VFF72_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HZV91698.1"
                     /translation="MTKKEILSMALLGALALPAAAQTLGRGGSLATGGAGPDGAQGAS
                     GQLEHCDSPKGTLAVVEPQNQVQASLQRYGLGSPTGVIRMLVQQSNCFQVVERGTGMR
                     SMMQERALAQGGELQNDQNIGKGQMVAADFIVTPSVVFSENNAGGIGGALGGLIGGGG
                     GRALAGLAGGVKFKQAETSMLLVDSRSGIQVASAEGSAEKADFAIGGAVLGGLSAAGG
                     GYGNTNEGKVVVASFLDNWNNIVRNIRNNPSLVQARAGAASQRNAAMSMRANAGAPGD
                     VMLPKINGVKVLRQARDGSGEVQTLTKSDEVLLSGEEMNGFVKVTTSRGEGWVKSILL
                     RKE"
     gene            complement(1805..2842)
                     /locus_tag="VFF72_00700"
     CDS             complement(1805..2842)
                     /locus_tag="VFF72_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HZV91699.1"
                     /translation="MSFTITVQPSGRAFSAERDEPILQAAIRQGVGLPYGCRDGACGS
                     CKCRLLEGRVIHGAHQQKALSHDEEASGLILACQAAPQTDIVLEARTVPGAGEFPVRK
                     LPSRVFSIDKPAADVAILRLQLPANDLLQYRAGQFVEFILRDGSRRSYSMANAPHTQL
                     DKPQIELHLRHLPGGKFTDHVFGAMKEKDILRVEGPFGSFFIREDSDKPIVLLASGTG
                     FAPIKAIIEHLVHKGSQRKAVLYWGCRSRADLYMHEWAEAATRQMAHLRYVPVLSDAK
                     PEDQWTGRTGLVHHAVMHDLPNLLEHQVYACGAPIMVESAQRDFIARCGLPDDEFYAD
                     SFTSEADKHGP"
     gene            2973..>3177
                     /locus_tag="VFF72_00705"
     CDS             2973..>3177
                     /locus_tag="VFF72_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZV91700.1"
                     /translation="MPDLPSRFRRPVLLIVGCGDIGLRVARSLAGRWRVFGLSSSPDR
                     FATLRAAGVTPLAGDLDRASTLAR"
BASE COUNT          446 a         1071 c         1122 g          538 t
ORIGIN      
        1 gaccgaacgg cccgcctcgg cgatggtcgg cacgtcgggc attgcgctcg atcgccgcgc
       61 ggtcgtcacg gcaagcgcct tcagtcgtcc ggccttgatg ttggcggcgg cggaggcgag
      121 gttgtcgaag gtcaggtcga cttggcccga cagcagggcg agctgggccg gatttccgcc
      181 cgcgtaggga atgtgcacca tgtgcacgcc ggcctcggcc ttgaacatct cgccggcgag
      241 atgacccgca ctgccgttgc ctcctgatcc gtagttcagc ttgccggggt tcttcttggc
      301 gtagtcgacg aggtcgacga cgctggcgat gccgagccgg gacgccgtct ccggcgtcat
      361 gacgaggacg ttcggaaccc gggcgacgcc ggtgatgggc gtgaagtcgc ggatcgggtc
      421 gtacggaagc cgggcgtaga gccaggggtt gatcgcgtgg gtggcgacgg cgcccatgac
      481 gagggtcagg ccgtccggcg cggccttggc aacgaagtcg gcaccgatgt tgcctccggc
      541 gccgggcttg ttctcgacga cgatcggtcc gaggctgtcc ttgacctttt cggcgagcgc
      601 ccgggccatg atgtcgagcg gacccccggc cgggtagggg acgatgaaat ggatcggccg
      661 ggcctgcgcc agtgagagac ccggcagcac tccgagggct ggagccgcga aggcggcgag
      721 agcgaggcga cgtttcattc cttcctcagc agaatcgact tgacccagcc ttcgccacgc
      781 gaagtcgtca ccttgacgaa gccgttcatt tcctcgccgc tcagcagcac ctcgtcggac
      841 ttggtcagcg tctgcacctc gcctgaaccg tcgcgtgcct ggcgcagcac cttgacgcca
      901 ttgatcttgg gcagcatcac gtcgcccggg gcgccggcgt tggcgcgcat gctcatggcc
      961 gcgttgcgct gcgatgcggc gccggcgcgc gcctgcacga gcgaagggtt gttgcggata
     1021 ttgcggacga tgttgttcca gttgtccaga aacgacgcga cgacgacctt gccctcgttg
     1081 gtgttgccgt agccgccgcc ggccgccgag aggccgccga ggaccgcgcc gccgatcgcg
     1141 aaatcggcct tctcggcgct gccctcggcc gatgcgacct ggatgccgga gcgcgagtcg
     1201 acgagcagca tgctcgtttc ggcctgcttg aacttcacgc cgcccgccag gccggccagg
     1261 gcccggccgc cgccgccgcc gatcaggccg ccgagggcgc cgccgatccc gccggcgttg
     1321 ttctcgctga agacaaccga gggagtgacg atgaagtcgg ccgcgaccat ctggcccttg
     1381 ccgatgttct ggtcgttctg cagctcgccg ccctgggcca gggcgcgctc ctgcatcatg
     1441 ctgcgcatgc cggtgccgcg ttcgacgacc tggaagcaat tcgactgctg caccagcatc
     1501 cggatcacgc cggtgggcga gcccaatccg tagcgctgca ggctggcctg cacctggttc
     1561 tgcggctcga ccaccgccag cgtgcccttg ggcgagtcgc agtgctcgag ctgtccgctg
     1621 gcgccttgcg cgccgtcggg gccggcgccg cccgtcgcga gcgagccgcc gcggccgagc
     1681 gtctgcgccg ccgcgggcag cgcgagggcc ccgagcaggg ccatcgacag gatctccttc
     1741 ttcgtcatgt cgtcctcctt cgtgccaacc ggcgaattgt ctgcctgccc cgcgtcaacg
     1801 gacgtcaggg tccatgcttg tcggcctctg aggtgaacga gtcggcatag aactcgtcgt
     1861 cgggcaggcc gcagcgggcg atgaagtcgc gctgtgccga ctcgaccatg atcggcgcgc
     1921 cgcaggcgta gacctggtgc tcgagaaggt tgggcaggtc gtgcatcacc gcgtggtgga
     1981 cgagcccggt gcgaccggtc cactggtctt cgggcttggc gtcggagagg accggcacgt
     2041 agcgcaggtg cgccatctgt cgcgtcgccg cctcggccca ttcatgcatg tacaggtcgg
     2101 cccgcgaccg gcaaccccag tagagcacgg cctttctctg gctgcccttg tgcacgaggt
     2161 gctcgatgat cgccttgatc ggcgcgaagc cggttccgga ggcgagcagc acgatgggct
     2221 tgtcggagtc ctcgcgaatg aagaagctgc cgaacggtcc ctcgacgcgc aggatgtcct
     2281 tttccttcat cgcaccgaac acatggtcgg tgaacttgcc gccgggaagg tggcgcagat
     2341 gcagctcgat ctggggcttg tcgagctgcg tgtgcggcgc gttggccatc gaatagctgc
     2401 gcctggatcc gtcgcgcagg atgaactcga cgaactgccc ggcccggtac tgcagcaggt
     2461 cgttggccgg caactgaagc ctgaggatgg cgacgtcggc ggcgggcttg tcgatcgaga
     2521 agacccggct cggcagcttg cggaccggaa actcgccggc accgggaacg gtgcgcgcct
     2581 cgagcacgat gtcggtctga ggcgccgcct ggcaggcgag gatgaggccc gaggcctcct
     2641 cgtcgtggct cagggccttt tgctggtggg cgccatggat gacgcggccc tcgagcagcc
     2701 ggcacttgca cgatccgcaa gcaccgtcgc ggcagccgta gggcaggccg acgccctggc
     2761 ggatcgccgc ctggaggatc ggttcgtcgc gctcggcgct gaaggcgcga ccgctcggct
     2821 ggacggtgat ggtgaacgac atgaaggacg acaacgacag gctctagact tcgccccacc
     2881 ccgccgccct cgaggcgcct gggcgccccg gcgccgggca gcccgcggtc gagcacgggg
     2941 gcgcacctct cgattttgcc ctcatcgtct ccgtgcccga tcttccgtcg cgattcagac
     3001 gcccggtcct gctgatcgtc ggttgcggcg acatcggatt gcgcgtcgcc cggtcgctcg
     3061 ccgggcgctg gcgggtcttc ggtctcagct cgagccccga tcgcttcgcg accttgcgcg
     3121 ccgccggagt gacgccgctc gccggagacc tcgaccgggc gtcgacgctc gcccggc
//
LOCUS       DASRWS010000041         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4043480, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000041 DASRWS010000000
VERSION     DASRWS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4043480"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFF72_00710"
     CDS             <1..358
                     /locus_tag="VFF72_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91701.1"
                     /translation="LLRVFQRLEASGTIRGGRFVNGLAGEQFALPEAIPVLREVRRHA
                     NDRTLVCVAATDPLNLAGTLLVGERVPAVAGNRLLFRDGVVVATLVAGKFWFDASVDA
                     EPAERERARASLARRF"
     gene            620..>2560
                     /gene="pdeR"
                     /locus_tag="VFF72_00715"
     CDS             620..>2560
                     /gene="pdeR"
                     /locus_tag="VFF72_00715"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HZV91702.1"
                     /translation="MNDDQRDQVLYARFGTSRPCWRLSADSNALQLTPVTGAGPAHVA
                     LDAAIALDPQQASQIRCLTGASSHLVVDVHLAGEPLRLHLVGKKIDSNTWSGTASAYE
                     DTESVVRDLAQALSFAEQVVSEVNSVVVVVDRHGRIQRFNRVAEELSGIKEETIIGST
                     AWALLLPSRDEASASANFARVFDRGVPYQIEQRIKTVNGERLFLFRNEFVRSGSDGDK
                     QFLVCSGTDITEQRLAQQRLTELANTDELTGLSNRNAIHDKIRAAIQDASPQEEVGVL
                     FLDLDNFKKVNDHYGHVFGDKLIRDVSAAIGDCLNEGDALARLGGDEFIVLAASGTSQ
                     QLETTAQRILERMRTPFAVGLVEVYTGCSIGIARYPQHGDSLESLIRSADTAMYVAKD
                     AGKRTHRVFSPEMNRRVAEYMWLDTNLRRGLEEGQLTLHYQPKLSLASGTVQGAEALV
                     RWNSPERGQIMPADFIRYAEESGLIGVLGRWVMESAAKQAARWKADGYKLRIAINMSA
                     RQLSDPAVVQHFTEALHNAELDPCLLDVELTESCLIEDEAAAIELITQFRQLGAQVHL
                     DDFGTGYSSLSQLGRIPLDVIKLDRSFVRSINADKKAQALVRSMVAVAQELDFEVVAE
                     GIETESEELFMKGLGVDYVQGFL"
BASE COUNT          494 a          821 c          802 g          443 t
ORIGIN      
        1 tctgctgcgg gtgtttcagc ggctcgaagc gagcggcacg attcgcggcg ggcgcttcgt
       61 gaatggcctg gctggcgagc agttcgcgtt gcccgaggcg attcccgtgc tgcgcgaagt
      121 tcgccggcat gcgaacgatc gtacgttagt gtgtgtggcc gcgactgatc cgttgaatct
      181 cgccggcacg ctgctggtcg gcgagcgggt gcccgctgtc gccggaaacc ggctgttgtt
      241 ccgggatggc gtagtggtgg ccacgctcgt cgccggaaaa ttctggtttg atgcgtcggt
      301 ggacgctgaa ccggccgaaa gggaacgcgc gcgcgcgtcg ctggcgcgta ggttctagcc
      361 tgtatgcgca cagggcacgt tagtgaacga agcgtggcat tggcacggca ggtcaggctc
      421 gcggacctta tggcgcccgc aatccgccag gaaagctgct cgagcgccca aaacccctaa
      481 agctattggg caatcgaccg ttaatgttta taagcccgaa ttcctccgct tcgctacaat
      541 gggccagtct taagtatgaa gactggatgc cgcctcgcgc cttgacgggg actaccggca
      601 cacatcgccc ggggatctca tgaacgacga ccaacgcgac caggtgcttt atgcgcggtt
      661 tggcacaagc aggccttgct ggcgtctgtc ggccgacagc aacgcactcc aactcacgcc
      721 tgtcaccggc gcagggcctg cccatgtagc gctcgatgcc gccattgcgc tcgatcccca
      781 gcaagcatcg caaatccgct gcctcacggg cgcaagctcg catctcgtcg tcgacgtgca
      841 cctcgccggc gaaccgctgc gcctgcatct cgtcggtaaa aaaatcgaca gcaacacctg
      901 gagcggcacc gcgtcggctt acgaggacac ggagtccgtc gtccgcgatc tggcacaggc
      961 cctgtcgttc gccgagcagg tcgtctcgga agtcaattcg gtcgtggtcg tcgtcgacag
     1021 gcatggccgg atacagcgct ttaaccgcgt tgccgaagag ttgagcggca tcaaggaaga
     1081 aaccatcatc ggcagcaccg cgtgggcgct attgttgccg tcccgggacg aggccagcgc
     1141 cagcgcaaat ttcgcccgag tcttcgatcg cggcgtgccc taccagattg aacagcgcat
     1201 taaaacggtg aacggcgaaa ggctgtttct gtttcgcaac gagttcgtgc gcagcggcag
     1261 cgacggcgac aagcagttcc tggtctgctc gggcaccgac atcaccgagc aaagactcgc
     1321 gcagcaacgg ctcaccgaac tcgccaacac cgacgagctg accggcctgt ccaaccgcaa
     1381 cgcgattcac gacaagatcc gcgcggccat ccaggacgcc agtccacagg aagaagtcgg
     1441 cgtgctgttc ctcgacctcg acaacttcaa gaaggtcaac gaccactacg gccatgtgtt
     1501 cggcgacaag ctgatccgcg acgtgtccgc cgccatcggc gactgcctga acgaaggcga
     1561 tgcgctcgcg cggctgggcg gcgacgagtt catcgtgctg gcggcaagcg gcacctcaca
     1621 acagctcgaa accaccgccc agcgcattct ggagcgcatg cgcacgccgt tcgccgtcgg
     1681 gctcgtcgag gtctacacgg gttgctcgat cggcatcgcg cgctatccgc agcacggcga
     1741 cagcctcgaa tcgctgattc gctcggccga caccgccatg tacgtcgcca aagacgcggg
     1801 caagcgcact catcgcgtgt tctcgccgga gatgaaccgc cgcgtcgccg agtacatgtg
     1861 gctcgacacc aatctgcgac gcggtctcga agagggccaa ctgacgctgc attaccagcc
     1921 gaagctgtcg ctcgcgagcg gcaccgtgca aggcgcagag gcgctggtgc gctggaattc
     1981 gccggaacgc gggcagatca tgccggcgga cttcatccgt tatgcggaag agtcggggct
     2041 gatcggcgtg ctgggccgct gggtcatgga atccgccgcg aagcaggccg ctcgctggaa
     2101 agcggacggc tataagctgc gcatcgcgat caacatgtcg gcgcggcaac tctccgaccc
     2161 cgccgtggtc cagcatttca ccgaagcgct gcacaacgcg gagctcgacc cctgcctgct
     2221 cgacgtcgaa ctcaccgaga gctgtctgat cgaggacgaa gcggccgcaa tcgaactcat
     2281 cacgcagttc cgccaactcg gcgcccaggt gcatctggac gacttcggca ccggctactc
     2341 gtcgctgtcg caactgggcc gcattccgct cgacgtcatc aagctcgatc gcagcttcgt
     2401 gcgctccatc aacgccgaca agaaggcgca agcactagtg cgctcgatgg tggcggtcgc
     2461 gcaggaactc gacttcgagg tggtcgccga aggcatcgaa accgagtcgg aagaactgtt
     2521 catgaagggc cttggcgtgg actacgtgca aggctttctg
//
LOCUS       DASRWS010000042         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1743571, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000042 DASRWS010000000
VERSION     DASRWS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743571"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFF72_00720"
     CDS             <1..591
                     /locus_tag="VFF72_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZV91703.1"
                     /translation="VPKSWADLLKPEYKGLVGYLDPASAFVGYVGAVAVNRARGGTLD
                     NFTPGIDYFKALMKNDPIVPKQTSYARVLSGEIAILLDFDFNAYRAKYKDGANVAFVI
                     PAEGTLVVPYVMSLVAHAPHADNGKKALDFVLSDEGQAIWARAFLRPVRASAMPKDVE
                     AKFLPASEYARAKSVDYDHMAKAQRGFSDRYLKEVR"
     gene            617..1438
                     /locus_tag="VFF72_00725"
     CDS             617..1438
                     /locus_tag="VFF72_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV91704.1"
                     /translation="MKTRSAGAAPTLLFACALPALVFFTAFWLLPAAKLIALPATSGW
                     RTYFVVVTSPRYLESMVDTLVLSLAVTATTLVLGAAVGIYLARRRFVGRGALISMLTL
                     PLSFPGVIVGFFVILLGGRQGLVPQLGASLGLTGLTFAYGLLGLFLAYIYFSLPRAIA
                     SYTAAAEAMDMQLEEAARAFGASRLRIVRDVWLPQLAPTSVACGAILFATAMGAFGTA
                     FTLASKVEVVPITIYNEFTNYANFALAASLSIALGLVTWAVLFVARHLGGDLRTA"
     gene            1445..2257
                     /locus_tag="VFF72_00730"
     CDS             1445..2257
                     /locus_tag="VFF72_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV91705.1"
                     /translation="MPTPSRSSPQQAAPLLFAVTLVVSLFMLVPIVISVFAGLVNNYS
                     HGLKSGLTLRWLAEVWSTYGDTVWASLLIATGAVASTLLLGVPCAYALARSRSRAAHV
                     FEELITLPVAVPGLATALALILVYGQIAAFRQSYAFILVGHVIFTLPFMVRIVSAAFQ
                     RPELAVLEEAARTLGAGFAQRFLGVLVPAVFPAIVAGTLMVFTLSIGEFNLTWMLRTP
                     LTRTLPIGLADSYASMRIEVGSAYTLVFLAVILPSLWALQAFAAALQRRYGN"
     gene            2279..>2419
                     /locus_tag="VFF72_00735"
     CDS             2279..>2419
                     /locus_tag="VFF72_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+/spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HZV91706.1"
                     /translation="MQLDRTRIEIVDVAKTYADGTRGLKPTTLTIEAGEVIALLGPSG
                     CGK"
BASE COUNT          330 a          901 c          751 g          437 t
ORIGIN      
        1 gtgccgaagt cgtgggccga cctgctcaag cccgaataca agggcctcgt cggatacctc
       61 gatccggcgt cggcgttcgt cggctacgtc ggcgcggtcg cggtcaaccg ggcgcgcggc
      121 ggcacgctcg acaacttcac gcccggaatc gactacttca aggcgctgat gaagaacgac
      181 cccatcgttc ccaagcagac ctcgtacgcg cgcgtgctct cgggagagat cgcgatcctg
      241 ctcgacttcg acttcaacgc ctatcgcgcg aagtacaagg acggcgccaa cgtcgcgttc
      301 gtgattcccg ccgaaggcac gctcgtcgtc ccgtacgtca tgagcctggt cgcccacgcg
      361 ccgcacgccg acaacggcaa gaaggctctc gacttcgtgc tgtccgacga aggccaggcg
      421 atctgggcgc gcgccttcct gaggccggtg cgggccagcg cgatgccgaa ggacgtcgag
      481 gcgaagttcc ttccggcctc cgagtacgcg cgggcgaagt cggtcgacta cgaccacatg
      541 gccaaggcgc agcgcggctt ttccgaccgc tacctgaagg aagtgcgctg agccgtcgcg
      601 gcgcggtgca tcgaccatga agacgcgctc cgccggcgcg gcgcccacgc tgctcttcgc
      661 gtgcgcgctg ccggcgctcg tcttcttcac cgcgttctgg ctgctgccgg ccgcgaagct
      721 catcgccctg cccgccacga gcggctggcg aacctacttc gtcgtcgtca ccagcccgcg
      781 ctacctcgag agcatggtcg acacgctcgt gctgtcgctc gccgtcacgg cgacgacgct
      841 cgtcctcggc gccgccgtcg gcatctacct ggcgcgccgc cgcttcgtcg gtcgcggcgc
      901 gctgatctcg atgctgacgc tgccgctgtc gtttcccggc gtcatcgtcg gcttcttcgt
      961 catcctgctt ggcggccgcc agggcctggt gccgcaactc ggcgccagcc tcggcctcac
     1021 cggcctgacc ttcgcctacg gcctgctcgg cctcttcctc gcctacatct acttctcgct
     1081 gccacgtgcc atcgcctctt acacggcggc cgccgaagcg atggacatgc agctcgagga
     1141 agcggcgcgc gccttcggcg cttcgcgcct gcgcatcgtc cgcgacgtct ggctgccgca
     1201 gctcgcgccg accagtgtcg cctgcggcgc gatcctcttc gccacggcga tgggcgcgtt
     1261 cggaaccgcc ttcacgctgg cgagcaaggt cgaggtcgtg cccatcacga tctacaacga
     1321 gttcaccaac tacgccaact tcgcactcgc cgcatcgctc tcgatcgcgc tcggcctggt
     1381 gacctgggcg gttctcttcg tggcacgtca cctcggtggc gacctgcgca ccgcctgatc
     1441 cgggatgccc acgccgagcc gatcctcccc gcaacaggcc gcgccgctgc ttttcgcggt
     1501 gacgctcgtc gtcagcctct tcatgctggt gccgatcgtg atctcggtct tcgccgggct
     1561 cgtcaacaac tacagccacg gcctgaagag cggtctcacg ttgcgctggc tcgccgaggt
     1621 atggagcacc tacggcgaca cggtctgggc gtcgctgctc atcgccaccg gtgccgtcgc
     1681 gtcgacgctg ctgctcggcg tgccgtgcgc ctacgcgctg gcgcgcagcc gttcgcgtgc
     1741 cgcgcacgtc ttcgaagagc tcatcacgct gccggtggcc gtcccgggcc tggcgacggc
     1801 gctcgccctc atcctcgtct acggccagat cgcggcgttc cggcagagct acgcgttcat
     1861 cctcgtcggc cacgtgatct tcaccctgcc gttcatggtg cgcatcgtct cggccgcttt
     1921 ccagcgcccc gagctcgccg tcctcgagga agccgcgcga acgctgggcg ccggatttgc
     1981 gcaacgcttc ctcggcgtgc tcgtgccggc ggtgttcccg gcgatcgtcg ccggcacgtt
     2041 gatggtcttc acgctgtcga tcggcgagtt caacctgacc tggatgctgc gcaccccctt
     2101 gacgcgcacg ctgccgatcg gcctggccga cagctacgct tcgatgcgca tcgaggtcgg
     2161 ctcggcctac accctggtct tcctggcggt gatccttcct tcgctctggg cgctgcaggc
     2221 cttcgcggca gccctgcaga gacgctacgg caactgaggc ggcacgggcc cattgaacat
     2281 gcaactcgac cgaacgcgca tcgagatcgt cgacgtcgcg aagacctacg ccgacggcac
     2341 ccgcggcctg aagccgacga cgctcaccat cgaagccggc gaagtcatcg ccttgctcgg
     2401 cccttcgggc tgcggcaag
//
LOCUS       DASRWS010000043         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1743634, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000043 DASRWS010000000
VERSION     DASRWS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743634"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2377)
                     /locus_tag="VFF72_00740"
     CDS             complement(<1..>2377)
                     /locus_tag="VFF72_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZV91707.1"
                     /translation="LVVLDGEPGVGKSRLAWEFEKYVDGLKAITRWHRGRCLSYGDGV
                     AFWALAEALRPRLGLTEADAGDAVAAGLEASLTEFVPRPEERDWLRPRLAALISGGQA
                     GGFAREDLFSAWTAFLERISQDGDTVVLVIEDAHYADDGLLAFLESMLANANAPIFVL
                     ALARPELLTRRPDLGGRRTSVVRLDPLDDPTMSTLVDGLVDGLPAAARTALVERAEGI
                     PLFAIETVRALIDQDAVVPRDGRYVPADGITIDLASIGAPASLQALVAARLDALSPGE
                     RAVVADASVLGTSFARAGLVALGSDDAGLDDALATLQRKEIIALQQDRFSADRGQYRF
                     VQSVVRQVAYATLSRRDRKARHLAAADFLSAQLDTNEDLAVVVAQHLLDAVDAGNPTD
                     DDAADLLPRACALLERAGSRARALGSPAEARRRFDAALGRATDPAARASLYLAAAEAA
                     YDAGDYEGAVAEAAAATELFDELGRPIDAALAVATGGMGLQILGDFAGVIRIAEPRWK
                     ALQGVTGAERALLRLARPLANASGQLENEEATRGYVERRVLLAEAVGDVGALAEAMIQ
                     LGTHYHGIGGPATARALYEAAADIARANDVPGTLARSLINIGTVLMSRDLLAAIDALR
                     EAREIARRSGVVAMIEFADGNYASTLWLAGRLSESRDVVAEAAATTATPSMQSLLACL
                     QANLADATGEPLPAAPHPAGTGGAWDRAGFGYLEMQRLLASGDAVAAAATTESTLVHV
                     LAAGGLDDDFCWLWPALVKAAVMAGDAPLAMRLLEPVTSASQGIISAAVAA"
BASE COUNT          404 a          839 c          791 g          343 t
ORIGIN      
        1 tctgcggcga ctgccgcaga gatgatgcct tgagaagcgc tcgtgaccgg ttcaaggaga
       61 cgcatcgcga gaggtgcgtc acccgccata actgccgcct ttaccagcgc gggccatagc
      121 cagcagaagt cgtcatcgag acctccggcg gccagcacgt ggaccagggt cgactccgtc
      181 gtcgccgctg cggcgaccgc gtcaccgctg gcgagcagcc gttgcatctc gaggtatccg
      241 aacccagcgc gatcccacgc acccccggta cccgcagggt ggggcgcagc tggcagcggc
      301 tcgccagtcg cgtcagcaag gttggcctgc agacaggcga ggaggctctg catagacgga
      361 gtcgccgtcg ttgcggcggc ctctgcgaca acatcgcgtg actcgctcag ccgtccggcg
      421 agccacaggg tcgacgcata gttgccgtcc gcgaactcga tcatcgcgac gacgcccgaa
      481 cggcgggcaa tctcacgcgc ttcgcgcagc gcatcgatcg ccgccagaag atcgcggctc
      541 atgagaaccg taccgatgtt gatcaacgaa cgcgccagcg ttcctggcac atcgttcgcc
      601 cgggcgatgt cggcggcagc ctcgtagagc gcgcgagcgg tagcgggccc gccgatgccg
      661 tgatagtgcg tgcccaattg aatcatcgcc tcggcgagcg cgccgacgtc tcctaccgcc
      721 tccgcgagta gcacccgccg ctccacgtac cctctggtgg cctcttcgtt ctcaagctga
      781 cccgaggcgt tcgcaagcgg cctcgccagc cggagcagcg cccgctcagc tcccgtgacg
      841 ccctgaagcg ccttccatcg tggttcggcg atgcggatga ccccggcaaa gtcgccaagt
      901 atctgcagac ccatgccgcc ggtcgcgacg gcgagcgccg catcgatggg ccgaccgagc
      961 tcgtcgaaca attcagtggc cgcagccgcc tcggcaacgg cgccttcgta gtcgccggcg
     1021 tcgtaagccg cctcggcggc ggccaggtag aggctggccc gcgcggccgg atcagtagcg
     1081 cgtccgagcg ctgcatcgaa ccgccgacgt gcttcggcgg gcgagcccaa cgccctggcc
     1141 cgggatccgg cccgctccaa aagcgcgcag gctctcggca acaagtccgc cgcgtcgtca
     1201 tctgtcggat tgccggcgtc gacggcgtcg agcaggtgct gcgcaacaac gaccgcgagg
     1261 tcctcgttcg tatcgagttg ggcggacaga aagtcggcgg ccgccagatg gcgcgccttg
     1321 cggtcgcggc gcgacaacgt ggcgtacgcg acttggcgaa cgaccgactg cacgaagcgg
     1381 tactggcccc ggtcagccga gaagcggtcc tgctgcaggg cgatgatttc cttgcgctgc
     1441 aacgtcgcca gcgcgtcatc gaggcctgca tcgtcggaac cgagcgccac cagccccgct
     1501 cgggcaaaag atgtgccgag cacgctcgcg tcggcaacca ccgcccgctc gcccggcgac
     1561 agcgcgtcga gtcgagccgc gacgagagct tgcagcgacg ctggagcgcc gatcgacgcg
     1621 aggtcgatcg tgatgccgtc ggccggcacg taccgcccat cccgcgggac gaccgcgtcc
     1681 tggtcgatga gtgcgcgcac ggtctcaatg gcaaagagcg gaatgccttc ggcacgctcg
     1741 acgagcgcgg tccgggcggc ggccggtaac ccgtcaacga gcccgtcgac aagcgtcgac
     1801 atcgtcgggt catcgagtgg atcgaggcgc accacgctgg tccgtcggcc accgaggtcc
     1861 ggccggcgcg ttaacagttc gggtcgcgcg agcgcgagca cgaagatggg cgcgttggcg
     1921 ttggcgagca ttgattcaag gaacgcgagc aacccgtcgt ccgcgtaatg cgcgtcttcg
     1981 atcaccagca caacggtgtc gccgtcttga ctgatccgct ccagaaatgc tgtccacgca
     2041 gagaaaaggt cctcgcgcgc gaacccgccg gcctgccccc cgctgatcag cgcggccagc
     2101 cgtggccgca gccagtcgcg ctcttcgggc cgcgggacga actcggtaag gctcgcctcg
     2161 agcccggctg cgacagcgtc gccggcgtcg gcttcggtga gaccaagacg tggccgcaat
     2221 gcctcggcca gcgcccagaa cgcgacgccg tcgccgtacg acagacagcg accgcgatgc
     2281 catcgggtaa tcgccttgag gccatcgacg tacttctcga actcccacgc gaggcgcgac
     2341 ttaccgacgc cgggttcgcc gtcgagcacg acgaggc
//
LOCUS       DASRWS010000044         6512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2319956, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000044 DASRWS010000000
VERSION     DASRWS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319956"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFF72_00745"
     CDS             complement(<1..937)
                     /locus_tag="VFF72_00745"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HZV91708.1"
                     /translation="MAVGTSSGGTVLVAGGGIGGLAAALALARRGFAVRVLEQAAEIG
                     EIGAGVQLAPNAFAAFDALGVGEAARSHAVYTERMVMMDAVDGTPVAEIPLGTAFRER
                     FGNPYAVIHRADAHRALLDGVLAHSGIDLCTSTRVVRFEQTAGDVNAIDAQGRVHRGL
                     ALIGCDGVKSAVREQLVGDDVRVSGHVVYRAVVDAAEFPADLAWNAPAVWAGPNCHLV
                     HYPLRGGEQYNVVVTFHSRDAETWGVREGSRDEVLSYFDGICDRARQLLRLPRSWKRW
                     ATADREPIEGWSSGRVTLLGDAAHPMLQYLAQGACM"
     gene            complement(948..2018)
                     /gene="gtdA"
                     /locus_tag="VFF72_00750"
     CDS             complement(948..2018)
                     /gene="gtdA"
                     /locus_tag="VFF72_00750"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HZV91709.1"
                     /translation="MDRIDTAHEAADAAIAATRKAFYQRIDGLDVAPLWEVLGALVPA
                     QPTSPVAPALWRYDELRKHMMEAGRLITAEEAERRVLVLENPALRGRSCITQSLFAGL
                     QLILPGEVAPAHRHTQTALRLVLDGEGAYTAVDGERTTMRYGDFIITPSWTFHDHGND
                     GAEPVVWLDGLDIPMVRFFDAGFREKSAARSQTATRPEGDTLARYGMNLLPLDHSPTP
                     DEPSRIFVYPFARTRESLLGISKGEVDAHHGFKLRFVNPATGRSPMPTIGTFAQRLPK
                     GFETRPYRSTDSSVQVCLEGGGRAVVGERVFDVGPRDIFVVPSWQPFAFAARDDSVLF
                     SFSDRPAQQVLGLWREERLEAR"
     gene            2112..3911
                     /locus_tag="VFF72_00755"
     CDS             2112..3911
                     /locus_tag="VFF72_00755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZV91710.1"
                     /translation="MTRMKHLSIVDGAFLHMESAEMPMHVGSLNLFEPPAGYSGEGFY
                     EAVKAHVARRMHLAPVFTRKLALMPFDLANPVWIHDDDIDLDYHVRYMVLPRPGTLAQ
                     LETLAARLHSMLLDRSRPLWEFYVIEGLADGRIGFYGKVHHAAVDGQAGVAMATSLFD
                     LTPEPRAVKPPREVRANTYQLGVAELLAAALQNQFQQVVQSLKLLPTLASTLTGAARE
                     ALAARRRESAEDRATRKAGKAPSPFTLAPSTPFNHSITNQRTFAAVSLPLGEIKAIGK
                     SVGASINDVVLWLCSTALRSYLKDSRELPGKSLVAAVPISLRQEGDTTANNQVAGTMI
                     GLGTEHADPLERLKAIKRGTSAMKRQMGTFRGVIPTDFPSLGSPWLISGLAALYGRSR
                     IADWLRIANVTISNVPGSRVPVYLVGARMTDYYPLSIVVHGVALNITVQSHVDELCFG
                     LIACRRAVPDLHELGEQLRKAMATLRQLAAAAAQALPAPAAIVDAIAPSAETRARAAK
                     ADTPAKKRASVARAPSERASAAKAPAARAPVARASAARAPTAPAPAARAPAVPDARAP
                     RLRVVPPTKSAAVRAKATPGAARRKPRAATGAR"
     gene            3920..4279
                     /locus_tag="VFF72_00760"
     CDS             3920..4279
                     /locus_tag="VFF72_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HZV91711.1"
                     /translation="MQIAAQVLVALVALLHVWFLVLEMFLWDKPLGLKTFRQTPESAA
                     TTKVLAANQGLYNGFLAAGLFWGLGLGAAGFEVKLFFLGCVIVAGLFGAATVGRKILY
                     VQALPAAVAMALVFVAR"
     gene            complement(4295..5866)
                     /locus_tag="VFF72_00765"
     CDS             complement(4295..5866)
                     /locus_tag="VFF72_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZV91712.1"
                     /translation="MIRSISSALRLGALCAGAALAGCASLPPPSPRVASAAALDTAET
                     QLGRLAAASLAGAGPGESGFRLLPTGDFAFDARTALIHRAERTLDVQYYQIADDSIGL
                     QLLRDLRDAAARGVRVRLLVDDLYTGGEDPLFRTFATLPNVEVRLFNPLPARGGNLYG
                     RLFFSLHDFSRINHRMHNKLFIADNRFAVSGGRNMADEYFMRSKLANFIDLDVISAGP
                     VVRELSAVFDRYWNSEPAWPVQDVVPLRVEPADASGRFDSLVRHAPAELLPASSGPLG
                     MTSVDDELAQGRLSLVFANAAVFADAPAKVGRGFRTPTATTVGTSVVEELQGARFEVQ
                     IASPYFIPGPTGMALLKEAIDHGVHVIVFTNSLGATDEPLVHFRYVRYRKAMLKLGVT
                     IYELSPDLVRRSGSFGDFGKSFGRLHAKVTVLDRKRVFIGSMNYDERSAWSNTESGLL
                     IESPELAGQILALTGRDRSESVYRLRLGPDDEVQWVVTAADGTERVLSEEPHRNWLLG
                     LKMFLLEPFASEDLL"
     gene            complement(6011..>6512)
                     /locus_tag="VFF72_00770"
     CDS             complement(6011..>6512)
                     /locus_tag="VFF72_00770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZV91713.1"
                     /translation="LSTTDALTEVGNRRLFDQTLKAEWARAARRHEPVGLLMIDIDHF
                     KDYNDHYGHPAGDACLRRVARMVGEAVRRGGELVARYGGEEFALLLPGADLDDARSAA
                     ERCRQIVGDAKIEHRASATSAWLTLSIGVASQTASAGVDCAVLVEVADAALYRAKRCG
                     RARIES"
BASE COUNT          982 a         2253 c         2333 g          944 t
ORIGIN      
        1 ccatgcaggc gccttgcgcg aggtactgca gcatcggatg cgcggcatcg ccgagcaacg
       61 tgacgcggcc gctgctccag ccctcgatcg gctcgcgatc ggcggtggcc cagcgcttcc
      121 agctcctggg caggcgcaac agctggcggg cccggtcgca gatgccgtcg aagtaagaga
      181 gaacctcgtc acgactgccc tcgcgaaccc cccaggtctc ggcgtcgcgg ctgtggaagg
      241 tgacgacgac gttgtactgc tcgccgccgc gcagcgggta gtgaacgagg tggcagttcg
      301 gcccggccca gacggccggc gcgttccagg cgaggtcggc ggggaattcc gcggcatcga
      361 cgacggcgcg atagacgacg tgccccgaga cgcggacatc gtcgcccacg agttgctcgc
      421 ggaccgccga cttgacgccg tcgcagccga tcagcgcaag gccgcgatgg acgcggcctt
      481 gcgcgtcgat cgcgttcacg tcgccggccg tctgctcgaa gcggacgacg cgggtcgagg
      541 tgcacaggtc gatgcccgaa tgggccagca cgccgtcgag gagggcgcgg tgggcgtcgg
      601 cgcgatggat caccgcatag ggattgccga agcgctcgcg aaacgcggtg ccgagcggga
      661 tctcggcgac cggcgtgccg tcgacggcgt ccatcatcac catccgctcg gtgtagacgg
      721 cgtggctgcg cgctgcttcg ccgacgccga gcgcatcgaa ggcggcgaag gcgttcgggg
      781 cgagctgcac gccggcgccg atctcgccga tctccgccgc ctgttcgaga acccgtaccg
      841 cgaagccgcg gcgggccagt gccaacgccg ccgccaggcc gccgatgccg ccgccggcga
      901 cgagcacggt gccgccggaa gacgtgccga ctgccatcgc ctcgacctca gcgcgcctcg
      961 agccgctcct cgcgccagag ccccagcacc tgctgggccg gccggtcgga gaagctgaag
     1021 aggacgctgt cgtcgcgcgc ggcgaaggcg aacggctgcc acgacggcac cacgaagatg
     1081 tcgcgcggcc cgacgtcgaa cacgcgttcg ccgaccaccg cccggccgcc gccttccagg
     1141 cagacctgga cgctcgaatc ggtgctccgg tagggccgcg tctcgaagcc cttcggcagg
     1201 cgctgcgcaa aggtgccgat ggtgggcatc ggcgagcggc cggtcgccgg gttgacgaag
     1261 cgcagcttga atccgtgatg cgcgtcgacc tcgcccttcg agatgcccag cagcgattcg
     1321 cgcgtgcgcg cgaacggata gacgaagatc cggctcggct cgtcgggcgt cggcgaatga
     1381 tccagcggca gcaggttcat gccgtagcgg gcgagcgtgt cgccttcggg gcgcgtcgcc
     1441 gtttgcgatc gggcggcgct cttttcccgg aagccggcgt cgaagaagcg gaccatcggg
     1501 atgtcgaggc cgtcgagcca gacgaccggc tcggcgccgt cgttgccgtg gtcgtggaat
     1561 gtccacgacg gcgtgatgat gaaatcgccg tagcgcatcg tcgtgcgctc gccgtcgacc
     1621 gcggtgtagg cgccttcgcc gtcgaggacg agacgaagcg cggtctgcgt gtgccggtgc
     1681 gccggcgcca cttcgccggg caggatcagt tgcagtccgg cgaacagcga ctgcgtgatg
     1741 cacgagcggc cgcgcagcgc cgggttctcg agcacgagga cacgccgctc ggcttcctcg
     1801 gccgtgatga ggcggccggc ttccatcatg tgcttgcgca actcgtcgta gcgccacagc
     1861 gctggcgcga ccgggctcgt cggctgcgcc ggcaccagcg cgccgagcac ctcccagagc
     1921 ggtgcgacgt cgagcccgtc gatgcgttgg tagaaggcct tgcgagtggc ggcgatggcg
     1981 gcgtctgccg cctcgtgcgc ggtgtcgatg cggtccatcc gcgcattgtg gatcaggcgc
     2041 accgggcaag cccgctggct gtcgcgggcg gcgcgcggta caacgcaagc ccgccgcgga
     2101 gggagagcgc catgacccgg atgaaacacc tgagcatcgt cgacggcgcc ttcctgcaca
     2161 tggaaagcgc cgagatgccg atgcacgtcg gcagcctcaa cctgttcgag ccgccggccg
     2221 gatacagcgg cgagggcttc tacgaggcgg tcaaggcgca cgtcgccaga cgcatgcacc
     2281 tggcgccggt gttcacgcga aagctcgccc tgatgccgtt cgacctggcc aacccggtct
     2341 ggatccacga cgacgacatc gacctcgact accacgtgcg ctacatggtg ctgccgcggc
     2401 ctggcacgct ggcgcagctc gagacgctcg cggcgcgcct gcattcgatg ctgctcgacc
     2461 gaagccggcc gctctgggag ttctacgtga tcgaaggcct cgccgatggc cgcatcggct
     2521 tctacggcaa ggtccaccat gccgcggtcg acggccaggc gggggtcgcg atggcgacca
     2581 gcctgttcga cctgacgccc gagccacgtg cggtcaagcc gccgcgcgag gtccgcgcca
     2641 acacctacca gctcggtgtc gccgagcttc tcgccgcggc gctgcagaac cagttccagc
     2701 aggtggtgca gagcttgaag ctcttgccca ccctggcatc gacgctcacc ggcgcggcac
     2761 gcgaagcact ggccgcgcgg cgtcgcgaaa gcgccgagga ccgcgcgacg cgaaaggccg
     2821 gcaaggcgcc gtcgcctttc acgctggcgc catcgacgcc gttcaatcat tcgatcacca
     2881 accagcgcac cttcgccgcg gtgtcgctgc ctctcggcga gatcaaggcg atcggcaagt
     2941 cggtcggcgc atcgatcaac gacgtcgtcc tctggctgtg cagcacggcc ctgcgctcgt
     3001 acctcaagga cagtcgcgag ctgcccggta aatcgctggt cgcggcggtg ccgatctcgc
     3061 tgcgccagga aggcgacacg acggccaaca accaggttgc cggaacgatg atcggtctcg
     3121 gcaccgagca cgccgatccg ctcgagcggc tgaaggcgat caagcgcggc acgtcggcga
     3181 tgaaacggca gatgggcacg tttcgcggcg tcatcccgac cgactttccg tcgcttggct
     3241 cgccgtggct gatcagcggc ctcgccgcgc tgtacggtcg ttcgcgcatc gccgactggc
     3301 tgcgcatcgc caacgtgacg atctccaacg tgcccggctc gcgggtgccg gtgtatctcg
     3361 tcggcgcgcg gatgaccgac tactacccgc tgtcgatcgt cgtccacggc gtcgccctca
     3421 acatcaccgt gcagagccac gtcgacgagc tctgcttcgg cctcatcgcc tgccgtcggg
     3481 cggtgcccga cctgcacgag ctcggcgagc agctgcgaaa ggcgatggcg acgcttcgcc
     3541 agctcgccgc ggcggcggcg caggcgttgc cggccccggc ggcgatcgtc gatgccatcg
     3601 caccgtcggc ggagacgagg gcccgtgccg ccaaggcgga cacgccggcg aagaagcggg
     3661 cgtcggttgc gcgggcaccg agcgaacggg cgtcggctgc aaaggcgccg gccgcacggg
     3721 caccggttgc acgggcatcg gctgcacggg cgccgaccgc accggcaccg gccgcacgcg
     3781 cacctgccgt gccggacgca cgggcgccgc gtctgcgcgt cgtgccgccc acgaagtccg
     3841 ccgctgtgcg ggcgaaggca acgcctgggg ccgccaggcg caagcctcgc gccgcgaccg
     3901 gcgcgcgctg agtcaggcca tgcagatcgc tgcgcaagtc ctggtcgccc tcgtcgcgct
     3961 cctgcacgtc tggttcctcg ttctcgagat gtttctctgg gacaagccgc tcgggctgaa
     4021 gacgtttcgc cagacgcccg agtcggccgc gacgacgaag gtgctcgccg ccaaccaggg
     4081 tctctacaac ggctttctcg ccgccggtct cttctggggc ctcggcctcg gcgccgccgg
     4141 cttcgaggtg aagctgttct tcctcggctg cgtcatcgtc gccggcctct tcggtgcggc
     4201 gacggtgggg cgcaagatcc tctacgtgca ggcgctgccg gcggcggtgg cgatggctct
     4261 cgtcttcgtc gcgcgctgag cgccgtggcg atgctcagag caggtcttcg gaagcgaagg
     4321 gctcgagcag gaacatcttc aggccgagca gccagttgcg gtgcggctcc tcggagagca
     4381 cgcgctccgt gccgtcggct gcggtgacga cccactgcac ctcgtcgtcg ggaccgagcc
     4441 gcagccggta cacgctctcg ctgcgatcgc gaccggtgag ggccaggatc tgtcccgcca
     4501 gctcggggct ttcgatgagc aggcccgatt cggtgttcga ccaggccgat cgctcgtcgt
     4561 agttcatcga gccgatgaag acgcgcttgc ggtcgagcac ggtcaccttg gcatgcagcc
     4621 ggccgaacga cttgccgaaa tcgccgaacg acccgctgcg tcgcacgaga tcggggctca
     4681 gctcgtagat cgtcacgccg agcttgagca tcgccttcct gtagcggacg tagcggaagt
     4741 gaaccagcgg ctcgtcggtg gcgccgagcg agttggtgaa gacgatgacg tggacgccat
     4801 ggtcgatcgc ttccttgagg agcgccatgc cggtcggccc gggaatgaaa tacggtgacg
     4861 cgatctgcac ctcgaaacgg gcgccctgca gctcctcgac gacgctggtc ccgaccgtcg
     4921 tcgcggtggg ggtgcgaaac cctcggccga ccttggccgg cgcatcggcg aagacggcag
     4981 cgttggcgaa gacgagcgac agtctgccct gagccagctc gtcgtcgacc gatgtcattc
     5041 cgagcggacc gctcgacgcg ggcagcagct cggccggggc gtgtcgaacc agcgaatcga
     5101 agcggcccga cgcgtcggcc ggctcgacgc gcagcggcac gacgtcctgc accggccacg
     5161 ccggctcgct gttccagtag cggtcgaaga cggccgagag ctcgcgcacc accggccccg
     5221 ccgaaatgac gtcgaggtcg atgaagttgg cgagcttgct gcgcatgaag tattcgtcgg
     5281 ccatgttgcg gccccccgag accgcgaagc ggttgtcggc gatgaacagc ttgttgtgca
     5341 tccggtggtt gatgcggctg aagtcgtgca gcgagaagaa gagccgcccg tacaggttgc
     5401 cgccgcgcgc cggcagcgga ttgaacagcc gcacctcgac gttgggcagc gtcgcgaagg
     5461 tgcgaaacag cggatcctcg cctccggtgt agaggtcgtc gacgagcagg cggacgcgca
     5521 cgccgcgcgc cgcggcgtcg cgcaaatcgc gcagcaactg caggccgatg ctgtcgtcgg
     5581 cgatctggta gtactggacg tcgagcgtgc gctcggcgcg gtggatgagc gcggtgcgcg
     5641 catcgaaggc gaagtcgccg gtcggcagca ggcgaaagcc cgattcgccc gggcccgcgc
     5701 cggccaggct ggctgctgcg agccggccca gctgcgtctc cgccgtgtcg agtgccgccg
     5761 cgctggcgac gcgcggcgac ggcggtggca ggctggcgca gccggcgagg gcagcgccag
     5821 cgcaaagggc gccgagccgg agcgcggaag aaatcgagcg gatcacgttg tgcggcggcg
     5881 tggccgccgt gcgtcgggcg gggacgaacc aggcaggggg cggattatgg cgaggcgccc
     5941 gagccgctgc acggtgcgag gctcgcgatc gctgccgcgt cggccgcggc cagtcggggc
     6001 ggctccgcgg tcaggactcg atgcgggccc ggccgcatcg cttggcccgg tacagcgcgg
     6061 cgtcggccac ctcgaccagc accgcgcaat cgacgccggc gctcgccgtc tggctggcga
     6121 cgccgatcga aagcgtcagc cacgccgacg tcgccgacgc gcgatgctcg atcttcgcgt
     6181 cgccgacgat ctgccggcag cgctcggcgg cactgcgggc gtcgtcgagg tcggcgccgg
     6241 gcagcagcag cgcgaattcc tcgcccccgt agcgcgccac cagctcgccg ccgcgacgca
     6301 ccgcctcgcc caccattcgc gcgacgcggc gaaggcaggc gtcgcccgcg ggatggccgt
     6361 agtggtcgtt gtagtccttg aagtgatcga tgtcgatcat cagcaggccg accggctcgt
     6421 gccggcgcgc ggcgcgcgcc cactcggcct tcagcgtctg gtcgaagagg cgccggttgc
     6481 cgacctcggt gagcgcatcg gtcgtcgaca gt
//
LOCUS       DASRWS010000045         6848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3756024, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000045 DASRWS010000000
VERSION     DASRWS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3756024"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VFF72_00775"
     CDS             <1..1277
                     /locus_tag="VFF72_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91714.1"
                     /translation="RDWASPSSEWSAVNSSSSKPRLRSLGLSIAAALLVASTALAQEA
                     SLRPEIGRPLKEAEALIKSQHYREALNKVREAQAAGPRSGTETYMVERMRMAAASGAG
                     DVDAAAQSFDSLNGSGRLSGAEKVRMLESLTVGYYRAGQYGKAIQWGQRYFKEGGTSP
                     SIRTLLIQSQYLGGDLAGAVRELTAEIQAAERAGSPPPEDRLKLLLNAATKQGDNNAV
                     VYAIEKLVTYYPKKEYWVDLMSRMQRKPTFSERLSLDAYRLSLATGSMTAASDYMDMA
                     QLALQANLPTESKQIVDKGFASGALGTGPEAGRHKRLRDLVDKQLAKDDAGRAQDEKD
                     ALAAKSGNDLVNVGMDLVYRGQAAKGVQLIQDGIAKGGLKRPEDAKLHLGIAQLAAGQ
                     KVKALATFKTVQGNDGTSDLARLWSLYARRAG"
     gene            complement(1361..2119)
                     /locus_tag="VFF72_00780"
     CDS             complement(1361..2119)
                     /locus_tag="VFF72_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZV91715.1"
                     /translation="MDIAGKVFIVTGGASGLGEGTVRMLAEHGGQVVVADLQAERGEA
                     VAEAARGRFVACDVSREDDAERAIAAALELGKLVGLVNCAGIAPAVKTVGKDGAHPLA
                     LFAKVVTVNLVGSFNMIRLAAEAMARNEPEVTGERGVLISTASVAAYDGQIGQAAYAA
                     SKGGIVGMTLPIARDLARSGIRNMTIAPGIFGTPMLFTMPAEVQQALAASVPFPSRLG
                     TPADYAKLVRHIIGNEMLNGEVIRLDGAIRLPPR"
     gene            complement(2137..2790)
                     /gene="adk"
                     /locus_tag="VFF72_00785"
     CDS             complement(2137..2790)
                     /gene="adk"
                     /locus_tag="VFF72_00785"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZV91716.1"
                     /translation="MRLILLGAPGAGKGTQAAVICRHFGIPQISTGDMLRAAVKAGTP
                     LGMAAKNVMDGGALVGDDIIIALVKERIREPDCASGFLFDGFPRTIPQADAMRTASVR
                     LDYVLEIDVPDSAIVERMGGRRVHVASGRTYHLRFNPPKKANCDDVTGEELIQRVDDQ
                     EDTVRKRLAVYAEQTRPLIAYYERWAASGDVDAPAYRRISGTGSVEDISARALAALR"
     gene            complement(2866..3627)
                     /gene="kdsB"
                     /locus_tag="VFF72_00790"
     CDS             complement(2866..3627)
                     /gene="kdsB"
                     /locus_tag="VFF72_00790"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZV91717.1"
                     /translation="MQFDVLIPARLASTRLPGKPLADIAGVPMIVRVARRAMRSQARS
                     VTVAADDAAIVDACTRHDVAALLTDPAHPSGSDRLAEAAIRLGLDGDDIVVNVQGDEP
                     MIDPVLIDACAGVLALRRECVMSTAAHPLRDAGDFENPNVVKVVLDRDGRALYFSRSP
                     IPWPRDGAAAGAPSPLRHVGLYAYRAAFLRRFPTLPPAPLEIIERLEQLRVLWHGERI
                     AVHVGEASSGIGVDTPQDLAKVRALFESNGGAGFA"
     gene            complement(3647..3844)
                     /locus_tag="VFF72_00795"
     CDS             complement(3647..3844)
                     /locus_tag="VFF72_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZV91718.1"
                     /translation="MQTRLLELLVCPVCKGPLQYLRPPQQATHELVCNADGLAFPVRD
                     GIPVMLEGEARVLDAAAAHEP"
     gene            complement(3825..4922)
                     /gene="lpxK"
                     /locus_tag="VFF72_00800"
     CDS             complement(3825..4922)
                     /gene="lpxK"
                     /locus_tag="VFF72_00800"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZV91719.1"
                     /translation="MTSRSTPGGFRGRLAAILERAWSGRGTVSNVLLPLAWIFGVVVA
                     IRRWLYQRGHLPSHTLPVPVVVVGNVVVGGAGKTPAVIAIVRLLREQGWTPGIVSRGH
                     GRPREDEVHRVRRESEAGVAGDEPIVLARRAGVPVFVGSDRVAACHGLLQAEPTVDII
                     VSDDGLQHLAMQREVEVIVFDERGVGNGRLLPAGPLRESLSPDATGRCGPSRLVLYSA
                     GVASTPWPGHLGRRELGAIASLADWWQARPTPAAALDALRSRRVLAAAGLARPRRFFD
                     MLRGAGFSLDELPLPDHYDFATLPWPSTAGDVIVTEKDAVKLDAGRRFGARVWVTALD
                     FETEAAFATALSACLPSRPPMTSEADANPPA"
     gene            complement(4937..5362)
                     /locus_tag="VFF72_00805"
     CDS             complement(4937..5362)
                     /locus_tag="VFF72_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZV91720.1"
                     /translation="MNFRRPQPEEPEINLIPFIDVLLVILIFLMLSTTYSKFTELQIS
                     LPVADADQARDRPNEIIVAVSADGRFSVDRRPVDGRSVEALTAAVAAAAGGSTDKILI
                     ISADATAAHQSVINVMDAARRAGLTKLTFATQSSAGASR"
     gene            complement(5359..5994)
                     /locus_tag="VFF72_00810"
     CDS             complement(5359..5994)
                     /locus_tag="VFF72_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZV91721.1"
                     /translation="MFSIIQAAGWPIWPLLICSIAALAVIIERLTSLRPPRVAPPRLL
                     DEVISVTRAHLPPPDIVNKLADNSVLGTVLAAGLRAVIAEPRITEPALRNTFETAGRA
                     AVHRLERYLNALGTIASAAPLLGLMGTVIGMIEIFGSQAPGGGNPTLLAHGISVALYN
                     TAFGLMIAIPSLMFYRYFRGLVDAYALGMEQAADRMVPHLMRFAMPRAAQT"
     gene            6248..6370
                     /locus_tag="VFF72_00815"
     CDS             6248..6370
                     /locus_tag="VFF72_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91722.1"
                     /translation="MKINSLRYSTASSEGISPNSTSIGAAVELSGRASNGFAHV"
     gene            6363..>6848
                     /locus_tag="VFF72_00820"
     CDS             6363..>6848
                     /locus_tag="VFF72_00820"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZV91723.1"
                     /translation="MFDEPGAARAARAWTVGELVAAVGAWVEDEFAACTVRGEISNFS
                     RAASGHCYFNVKDADGAATLRCAMFRRAAQLASFQPRDGDAVALRGRLAIYEPRGELQ
                     FIVESVQRSGAGELYERFLRLKAKLDAEGLFAAGLKRPVPAHPVTVGVVTSLAGAALH
                     DV"
BASE COUNT         1144 a         2214 c         2419 g         1071 t
ORIGIN      
        1 gcagagactg ggcgtcacca agctcggaat ggtcggcagt gaacagttcg tcaagtaagc
       61 cgaggcttcg cagtctcggc ctgtcgatcg cggctgcgct tttggttgcc agcaccgcgc
      121 tggcgcagga ggcgtcgctg cggccggaaa tcggcaggcc gctcaaggag gccgaggcgc
      181 tcatcaagtc gcagcactac cgcgaggccc tgaacaaggt ccgcgaagcg caggcggcgg
      241 ggccgagaag cggcaccgag acctacatgg tcgagcggat gcgcatggcg gccgcctcgg
      301 gcgccggcga cgtcgacgcg gcggcgcaat ccttcgacag cctgaacggc agcggcaggc
      361 tgtcgggcgc cgagaaggtg cgcatgctcg agtcgctgac cgtcggctat taccgggccg
      421 gccagtacgg caaggcgatc cagtggggtc agcgctattt caaggaaggc ggaaccagcc
      481 cgtcgattcg caccttgctc attcaaagcc agtatctcgg gggcgatctc gcaggcgccg
      541 tccgggagct gacagccgag atccaggcgg ccgagcgcgc cggatcgccg ccgcccgaag
      601 accgcctgaa gcttctcctc aacgccgcca ccaagcaagg cgacaacaac gccgtggtct
      661 acgccatcga gaagctggtg acctactacc cgaagaagga gtattgggtc gacctgatga
      721 gccggatgca gcgcaagccg acgttctcgg agcggctctc gctcgacgcg tatcgactgt
      781 cgctcgcaac cggcagcatg acggccgcgt ccgactacat ggacatggcg cagctcgcct
      841 tgcaagccaa cctgccgact gaaagcaagc agatcgtcga caagggattc gcgtcgggtg
      901 cgctgggcac cggtccggag gccgggcgtc acaagcgtct gcgcgacctc gtcgacaagc
      961 agctcgccaa ggacgatgcg ggccgtgcgc aggacgagaa ggacgcgctg gcggcgaagt
     1021 ccggcaacga tctcgtgaac gtcggcatgg atctggtcta tcgaggccag gctgcaaaag
     1081 gcgtccagct catccaggac gggatcgcca agggtggcct gaagcgaccc gaggacgcga
     1141 agctgcatct gggcatcgcg cagctcgcgg cgggacagaa ggtgaaggcg ctggcgacgt
     1201 tcaagaccgt tcagggcaac gatggaacga gcgacctggc gcgcctttgg tcgctttacg
     1261 cgcgacgcgc agggtgacgg cgaggagccg cgcaagctct gccttcctcc tggaggggag
     1321 aaccggccag cgcgcccctg acgcgatccg gctcctgccg ctaccgaggc ggtagccgga
     1381 tcgcgccgtc gaggcggatc acctcgccat tgagcatttc gttgccgatg atgtgacgga
     1441 cgagcttggc gtagtcggcc ggcgtcccca gccggctcgg gaagggaacg ctcgccgcga
     1501 gcgcctgctg gacctcggcg ggcatcgtga acagcatggg cgtgccgaag atgcccggtg
     1561 cgatcgtcat gttgcgaatg ccgcttcgtg ccaggtcgcg ggcgatcggc agtgtcatgc
     1621 cgacgatgcc gcccttcgaa gcggcatagg cggcctggcc gatctggccg tcgtaggcgg
     1681 caacactcgc cgtcgagatc aggacgcctc gctcgccggt gacttcaggc tcgttgcgcg
     1741 ccatcgcttc ggcagcgagg cggatcatgt tgaagctgcc gacgaggttg accgtcacga
     1801 ccttcgcgaa gagcgcgagc ggatgagcgc cgtccttgcc gacggtcttg accgccgggg
     1861 cgatgccggc gcaattgacg aggccgacca gcttgccgag ctcgagcgcc gcggcgatcg
     1921 cccgctcggc gtcgtcttcg cggctgacgt cgcaggcgac gaagcggccc ctggctgcct
     1981 cggcgaccgc ctcgccccgc tcggcctgca gatcggcgac gacgacctgg ccgccatgct
     2041 cggcgagcat ccggaccgtg ccttcgccga ggcccgatgc gccgccggtg acgatgaaga
     2101 ccttgcctgc gatgtccatg tcttgctccg ttcgattcag cgcagcgccg ccagggcgcg
     2161 tgcgctgatg tcctcgacac tgccggtgcc gctgatgcgt cgatacgccg gcgcatcgac
     2221 gtcgcccgaa gccgcccagc gctcgtagta ggcgatcagc ggccgggtct gctcggcgta
     2281 gacggccagg cgtttcctta ccgtgtcctc ctggtcgtcg acgcgctgga tcagttcttc
     2341 gcccgtcacg tcgtcgcagt tcgccttctt cggtgggttg aagcgaaggt ggtaggtgcg
     2401 accggatgcg acgtggacgc gacgtccgcc catcctctcg acgatggcgc tgtcgggaac
     2461 atcgatctcg aggacgtagt cgaggcgaac gctggccgtc ctcatggcgt cggcctgggg
     2521 aatcgtccgc gggaagccgt cgaacaggaa tcccgaggcg caatcgggct cgcggatgcg
     2581 ttccttgacg agagcgatga tgatgtcgtc gccgaccagc gcgccgccgt ccatgacgtt
     2641 tttcgcggcc attccaagcg gcgttcccgc cttgacggcg gcgcgcagca tgtcgccggt
     2701 cgatatctgc ggaatgccga aatgccggca aatgaccgct gcctgcgttc ccttgcccgc
     2761 gccgggcgcg cccaacagga tcagtctcat ggtcggtcga attcgtttcg ggccggcaac
     2821 gagggcatca tagggggcga cagcgggatg cccgagtggt tgcgctcagg caaaacccgc
     2881 accgccattg gactcgaaca gtgcgcgcac cttcgcgagg tcttgcggcg tgtcgacgcc
     2941 gatgccggag ctggcttcgc cgacatgaac cgcgatccgt tcgccatgcc aaagcacgcg
     3001 caactgctcg agtcgctcga tgatctccag cggcgccggt ggcagcgtcg ggaaacggcg
     3061 caggaaggcg gcgcgatagg catacaagcc gacatgtcga agtggcgatg gcgcgcctgc
     3121 agccgcgcca tcgcgcggcc agggaatcgg tgaacgcgag aaatagaggg cacgtccgtc
     3181 gcggtcgagg acgaccttga cgacgttcgg gttctcgaag tccccggcgt cgcgaagcgg
     3241 atgcgcggcg gtgctcatca cgcattcgcg tcgcagcgcg agtacgcccg cgcaggcgtc
     3301 gatgaggacc gggtcgatca tcggctcgtc gccctggacg ttgacgacga tgtcgtcgcc
     3361 gtccaggccg aggcgaatcg ccgcttcggc gagccggtca ctgccgctgg ggtgcgcagg
     3421 atccgtgagc agagccgcga catcatgtcg cgtgcaggcg tcgacgatcg ccgcgtcgtc
     3481 ggcggccacg gtgacagatc gcgcctgcga ccgcatggcc cgccgggcga cgcggacgat
     3541 catcggcact ccggcgatgt ccgcgagcgg tttgcccggc agtcgggtcg aagcgagccg
     3601 ggccgggatc aggacgtcga attgcaagcg cgattcgccg gagcggctag ggttcgtgcg
     3661 cggccgcggc gtcgagaacg cgggcctcgc cctcgagcat caccggaatg ccgtcacgca
     3721 ccgggaaggc gagcccgtcg gcgttgcaga cgagctcgtg cgttgcctgt tgcggcggtc
     3781 gcaggtattg cagcggcccc ttgcagacag gacagacgag gagctcaagc aggcgggttt
     3841 gcatcggctt cggaggtcat cggtggtcgg gagggcaggc aggcagacag tgcggtggcg
     3901 aatgccgcct cggtctcgaa gtctagcgcc gtaacccaga cacgcgcgcc gaagcgacga
     3961 cccgcgtcga gcttgacggc gtccttctcg gtgacgatca cgtcgccggc cgtcgatggc
     4021 cagggcagcg tcgcgaaatc gtagtgatcg ggcaagggca gctcgtcgag gctgaatcct
     4081 gcgccccgga gcatgtcgaa gaagcggcgc ggccgggcca gcccggccgc ggcaagaacg
     4141 cgtctcgatc tcagcgcgtc gagcgcggct gcgggcgtcg gccgcgcctg ccaccagtcg
     4201 gcgaggctgg cgatggcgcc gagctcgcgg cgcccgaggt gccccggcca gggcgtcgag
     4261 gcgacgccag cgctatagag gacgagacgg ctcggcccgc accgtcccgt ggcgtcagga
     4321 gagagcgatt cgcgcaacgg gccggcggga agcagccggc cgttgccgac gccacgctcg
     4381 tcgaagacga tcacctcgac ctcgcgctgc atcgcgaggt gctgcaagcc gtcgtcgctg
     4441 acgatgatgt cgaccgtcgg ctccgcctgg agcaagccgt ggcaggcggc gacccggtcg
     4501 ctgccgacga agaccggcac gccggcacgg cgggcgagaa cgatgggttc gtctccggcc
     4561 accccggctt cgctctccct tcggacgcga tgcacttcgt cctcgcgcgg tcgaccgtgg
     4621 ccgcgagaca cgatgcccgg tgtccagccc tgttcgcgaa gcagtcggac gatggcgatc
     4681 accgccggcg tcttgccggc gccgccgacc accacattgc cgacgacgac gacgggaacc
     4741 ggcagcgtgt ggctcgggag atggcctcgt tggtacagcc agcgacgaat ggcgaccacg
     4801 acgccgaaga tccatgccaa cggaagaaga acgttgctga cggtgccgcg tccggaccac
     4861 gcccgttcca ggatggcggc gaggcggccg cgaaagccgc cgggcgtcga ccgcgacgtc
     4921 atcgcccgac gccggctcag cgcgacgcgc cggcagagga ttgcgtcgcg aatgtcagct
     4981 tcgtcaaccc ggcgcgacgc gcggcgtcca tgacgttgat gacgctctgg tgtgcggcgg
     5041 tggcgtcggc cgagatgatg aggatcttgt cggtggaacc gcctgccgcg gcggcgaccg
     5101 cggccgtcag ggcttcgacg cttcttccgt ccacgggcct gcgatcgacg gagaagcgcc
     5161 cgtcggccga caccgcgacg atgatctcgt tcgggcggtc gcgtgcctgg tcggcatcgg
     5221 cgacgggcag gctgatctgc aattcggtga acttcgaata cgtcgtcgac agcatcagga
     5281 agatgaggat gacgagcagc acgtcgatga acgggatcag gttgatctcc ggttcttcag
     5341 gctgcggcct gcggaaattc acgtctgcgc ggcccgtggc atggcgaagc gcatcaggtg
     5401 cgggaccatg cgatcggcgg cctgctccat gcccaatgca tacgcatcga ccaggccgcg
     5461 gaaatatcgg tagaacatca gcgaaggaat ggcgatcatg agcccgaatg cggtgttgta
     5521 gagcgccacc gaaatgccgt gggcgagcaa ggtgggattg ccgccaccgg gcgcctgcga
     5581 tccgaagatc tcgatcatgc cgatcacggt gcccatcagg ccgagcaggg gcgccgccga
     5641 ggcgatggtg cccagggcgt tgaggtagcg ctcgagccgg tgcaccgccg cccggccggc
     5701 cgtctcgaag gtattgcgca gcgccggctc ggtgatccgc ggctcggcaa tcacggcgcg
     5761 caaacccgcg gccaggacgg tgcccaggac ggagttgtcg gcgagcttgt tgacgatgtc
     5821 cgggggcggc aggtgggcac gtgtcacgga gatgacctcg tcgagtagac gaggcggcgc
     5881 cacccgaggc gggcgcaggc tcgtcagccg ttcgatgatc actgccagtg cggcgatgga
     5941 gcagatgagc agcggccaga tgggccatcc tgccgcttgt atgatcgaaa acaaaatggc
     6001 cctttcctgt ggccgccagc gcgacccgtc gtgtgtccgc agcgcgcgga ttatggcgtg
     6061 tggttcgcgt cgatctaccc gaggcgacct gtccacgtcg gcaatccgtg cggcgtgcga
     6121 cacgctcgcc acgatcatcc actgtcgccg tggacaactt tgtggacaac cgtctgtgcg
     6181 atcgtcgaat cttcaacaac catgcggcag aggttggatt gctgaagaat tgagcaggaa
     6241 taatgccttg aaaatcaaca gcttgcgata ttcgactgcg tcgtccgaag gcatctcgcc
     6301 gaattccacc agcattggcg cggctgtgga gttgtcgggc cgcgccagca atggctttgc
     6361 gcatgtttga cgagcctggc gccgcgcgcg ccgcccgcgc gtggaccgtc ggcgagctcg
     6421 tcgccgccgt cggcgcatgg gtcgaggacg agttcgcggc ttgcaccgtt cgtggcgaga
     6481 tctcgaactt ctcgcgagcg gcgagcggtc attgctactt caatgtgaag gacgccgacg
     6541 gcgcggcgac gttgcgttgc gcgatgtttc gccgtgccgc ccagctcgct tccttccagc
     6601 cgcgcgacgg cgacgccgtc gccctgcgag gccgcctggc aatctacgag ccgcggggcg
     6661 aactgcagtt catcgtcgag tcggttcagc gaagcggagc cggcgagcta tacgagcggt
     6721 tcctgcgcct gaaggcgaag ctcgacgccg agggtctgtt cgccgctggc ctcaagcgcc
     6781 ccgtgcctgc gcatcccgtg accgtcggcg tcgtcacgtc tctcgccggc gctgcgctgc
     6841 acgacgtt
//
LOCUS       DASRWS010000046         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2606552, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000046 DASRWS010000000
VERSION     DASRWS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606552"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="pyrF"
                     /locus_tag="VFF72_00825"
     CDS             complement(<1..694)
                     /gene="pyrF"
                     /locus_tag="VFF72_00825"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZV91724.1"
                     /translation="MSFVSMLAAAERANDSMLCVGLDPDPARFPGPLRGDDSRILEFC
                     SRIVDATRDLVIAFKPQIAYFAAHRAEAQLERLVAHIRKSAPGIPVILDAKRGDIGAT
                     AENYAREAFERYDADAVTLSPFLGFDSLEPYLRRPGKGAILLCRTSNAGGADLQEQRL
                     ASGDLLFEHIARLASGPWNESGQLGLVVGATYPSEIARIRELAPTVPLLIPGVGAQGG
                     DAGATVRAGWRGT"
     gene            complement(691..2085)
                     /gene="purB"
                     /locus_tag="VFF72_00830"
     CDS             complement(691..2085)
                     /gene="purB"
                     /locus_tag="VFF72_00830"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZV91725.1"
                     /translation="MPVSPLTALSPLDGRYAGRLDPLRPLLSEFGLIRCRVRVEVEWF
                     IALSEAGFDELPRLDDDSLATLRRIVDVFDERDAESIKAIERTTNHDVKAVEYWLAER
                     ISGEPALAPIAGFLHFACTSEDINNTSHALMLEAARRDLLLPALDALVATLSARAVEL
                     AAQPMLSRTHGQPATPTTLGKEIANVVARLAHARERIAGVRLLAKMNGAVGNYNAHLA
                     AYPDVDWEAFARRVVEERLGLAFNPHTTQIEPHDAIAELFDAIKRCNTILIDWSRDAW
                     GYISLGYFTQKTKAGEIGSSTMPHKVNPIDFENGEGNFGIANALLAHMSEKLPVSRFQ
                     RDLTDSTVLRNMGVAFGHTLLGLDSLARGMAKIGVDAAALDADLDSAWEVLAEPVQTV
                     MRRYGVANPYEQLKELTRGKSITREGLHAFIRGLPIPAGERDRLLAMTPRGYTGLAAT
                     LARRTGASPSPEPR"
BASE COUNT          353 a          715 c          833 g          338 t
ORIGIN      
        1 ccgtgccgcg ccagccggct cgcaccgtcg cacccgcgtc gccgccctgc gcgccgacgc
       61 cggggatgag cagcggcacc gtcggcgcca gctcgcggat gcgggcgatc tccgaagggt
      121 aggtcgcacc gacgaccagg ccgagctggc cgctttcgtt ccatggcccc gaggcgagcc
      181 gcgcgatgtg ctcgaacagc agatcgccgg aggcgaggcg ctgctcctgc agatcggcgc
      241 cgccggcgtt cgacgtgcga cagagcagga tcgccccctt gccgggccga cgcagatagg
      301 gctcgagcga gtcgaagccg agaaacggcg acagcgtcac cgcgtcggcg tcgtagcgct
      361 cgaacgcttc ccgggcgtag ttctcggcgg tggcgccgat gtcgccgcgc ttggcgtcga
      421 ggatgaccgg gatgcccggg gcgctcttgc ggatgtgggc gacgaggcgc tcgagctgcg
      481 cttcggcgcg atgcgcggcg aagtaggcga tctgcggctt gaaggcgatg acgagatcgc
      541 gcgtcgcatc gacgatgcgg ctgcagaact ccaggatccg cgagtcgtcg ccgcgcagcg
      601 gcccgggaaa gcgggccgga tcggggtcga ggccgacgca cagcatcgaa tcgttcgccc
      661 gctccgccgc ggcgagcatc gagacgaagc tcatcgcggt tcgggagacg gagatgcgcc
      721 cgttcggcgg gcgagcgtgg ctgccaggcc ggtgtagccg cgcggcgtca tcgccagcag
      781 gcggtcgcgc tcgccggccg gaatcggcag gccgcggatg aaggcgtgca ggccctcgcg
      841 ggtgatcgac ttgcctcgcg tcaattcctt gagctgctcg tacggattgg cgacgccgta
      901 gcggcgcatg accgtctgca caggctcggc gaggacctcc caggcgctgt cgagatcggc
      961 gtcgagcgcc gcggcgtcga cgccgatctt ggccatgcct cgggccagcg aatcgaggcc
     1021 gagcagcgtg tggccgaagg cgacacccat gttgcgaagc accgtgctgt cggtgaggtc
     1081 gcgctggaag cgcgagacgg gcagcttctc gctcatgtgc gccagcagcg cgttggcgat
     1141 gccgaagttg ccttcgccgt tctcgaagtc gatcggattg accttgtgcg gcatcgtgct
     1201 cgagccgatc tcgcccgcct tcgtcttctg cgtgaaatag cccaggctga tgtagcccca
     1261 ggcatcgcgc gaccagtcga tcaggatggt gttgcagcgc ttgatcgcgt cgaacagctc
     1321 ggcgatggcg tcgtggggct cgatctgcgt cgtgtgcgga ttgaaggcga ggcccagccg
     1381 ctcctcgacg acccgccgcg cgaaggcctc ccagtcgaca tccggatagg cggcgagatg
     1441 ggcgttgtag ttgccgacgg cgccgttcat ctttgccagc agcctgacgc cggcgatgcg
     1501 ttcgcgggcg tgagcgagcc gagcgacgac gttggcgatc tccttgccga gggtcgtggg
     1561 cgtggccggc tggccgtgcg tgcgcgacag catcggctgc gccgccagct cgacggcgcg
     1621 ggccgacagg gtggccacga gagcgtcgag cgccggtagc agcaggtcgc gccgggccgc
     1681 ctccagcatc agcgcgtggc tggtgttgtt gatgtcctcg ctggtgcagg cgaagtgcag
     1741 gaagccggcg atgggggcca gcgccggctc cccggaaatg cgctcggcca gccagtattc
     1801 gaccgccttg acgtcgtggt tggtggtgcg ctcgatcgcc ttgatcgact cggcgtcgcg
     1861 ctcgtcgaag acatcgacga tgcgtcgaag cgtggcgagc gagtcatcgt cgagacgagg
     1921 gagctcgtcg aagccggcct cggacaaggc gatgaaccac tcgacctcga cgcgcacccg
     1981 gcaacggatc aggccgaact ccgacagcag ggggcgaagc ggatcgagcc tgcctgcgta
     2041 gcggccgtcc agcggcgaaa gggcggtgag cggcgaaacg ggcatcggcg ggaacgaacg
     2101 cgaaataacg gcgagccggt cattatcggt gcccctagaa tgaatcggac gtcgatttat
     2161 cgggccgacg ggactacgcg gacaaggcgg ggcggcgaga catgaaactg atcggatccc
     2221 tcaccagccc gtacgtgcg
//
LOCUS       DASRWS010000047         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1746216, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000047 DASRWS010000000
VERSION     DASRWS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746216"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VFF72_00835"
     CDS             complement(<1..1204)
                     /locus_tag="VFF72_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZV91726.1"
                     /translation="MSETLDPLRVPLCGSRLIEASAGTGKTWTIAGLYLRLVLGHGES
                     GAAFTRPLVPAEILVMTFTVAATRELSDRIRSRLVEAARCFRGEREPASDDAFLRALI
                     DDYASGEARAAAARRLSTAAESMDDAAVLTIDAWCQRMLREHAFDSGSLFDEELLADE
                     FAFVDEAARDYWRQNIYPLAGASLDAALAVWADVDRLVADVRELQAYVDVDGVEADLS
                     LDAARLRLVANSSQTVRRLKTGWVERAAQMRRWLEAQLGADPCHFNRSKLRADYVTTW
                     CNALDAWAVSPELARPGLSESAWRRLSAEGLLDAHKGSAPLAIPSWADELSALRAELS
                     GLPVIEHAMRMHAAATVKARVSALKRSAGTLGFRDMLERLDAALGGPNAERLRARIVA
                     QYPAALIDE"
     gene            complement(1197..>3710)
                     /gene="recC"
                     /locus_tag="VFF72_00840"
     CDS             complement(1197..>3710)
                     /gene="recC"
                     /locus_tag="VFF72_00840"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HZV91727.1"
                     /translation="RQGRDFMRQLDAFDDAERSRNLFDVPRIDLFEEGAGAHMLEQVQ
                     AMIRDLVPLAEHPARAAAPSDRSIVFHVAHNPQREVEVLHDQLLALLADTGSTQPLRP
                     RDIVVMVPDIDTFAPAIRAVFGQHAPGDARRIPFGIVDIRNRGNDPLVVALEWLLRAP
                     AQRFRASEVKDLLEVPAIARRFGLEDADLPRLAGWIEGAGVRWGLDAAQRAGLGLEAS
                     GVQNTWRFGLRRMLLGYASGAAFAGVEPYDEIGGLEAALVGALADLVDRLGEWCAQCQ
                     SAATAREWARRGRELLAALVEPADESERLTLAALESALAEWLEACELAGFDEPVPLAV
                     FREAWLSAVDPPGGARRFLAGGVTFCTLMPLRAVPFEVVCLLGMNDGAYPRQSRRSDF
                     DLMGLPGQQRPGDRSRRDDDRYLMLEALLSARRVLYVSWSGRNARDNGDEPPSVLVSQ
                     LRDYLRAGWSGNAAEDAVALRTTEHPLQPFSSRYFEGRGLFTYAREWRSAHRRGTEPD
                     VTAPSMPAFERRDAPLTIDELARFLRNPVKTFFRVRLGVAFDDEEAANYDDETFVLDG
                     LDRYAMLCDLIDESPGRPSEGSDESLDRRLQRIQGAGRLPIGEAASRLGSELAGIAAP
                     MLSERRRFQSRFADPAPPRHLHFEADGVVVDDWLDGLQSDGTLDAWLRVRPSALADSD
                     GVQPSADKLIDAWVRVLVSSACGCAAPGVYVGRDVTLRLGTLDQPDAAEALRHLLAAW
                     RRGMEEPLPLPARTAIAFVLGENAELMYDGAFDRDGERSEPCLGRCFPDFAALNADGG
                     FKALAERIYRPMLDWIAARVEVLQREAEAVPRAEEAPHV"
BASE COUNT          588 a         1318 c         1280 g          524 t
ORIGIN      
        1 actcgtcgat cagcgcggcc ggatattgcg cgacgatgcg tgcgcgcagc cgctcggcgt
       61 tcgggccgcc gagcgcggcg tcgaggcgct ccagcatgtc ccggaagccg agcgtgccgg
      121 cgcttcgctt cagcgccgag acccgcgcct tgacggtcgc ggcggcgtgc atgcgcatcg
      181 cgtgctcgat gacgggcaag ccagagagct ccgcgcgcag cgcggagagc tcgtcagccc
      241 atgacgggat agcgagaggt gccgatccct tgtgcgcgtc cagcaggccc tccgccgaga
      301 ggcgtcgcca ggcgctctcg ctcaggccag gccgcgccag ctccggcgac acggcccacg
      361 catccagcgc attgcaccag gtcgtcacgt agtcggctcg gagcttggaa cgattgaaat
      421 gacacgggtc cgcgcccagc tgcgcttcga gccagcgacg catctgcgcg gcgcgctcga
      481 cccatccggt cttcaggcga cgcactgtct ggctcgaatt cgctacgagc cgcagacgtg
      541 ccgcgtcgag cgagagatcg gcctcgacgc cgtcgacgtc gacgtaggct tgcagttccc
      601 tcacatccgc cacgagccga tcgacatcgg cccacaccgc cagcgcggca tcgagcgagg
      661 cgccggcgag cggatagatg ttctgccgcc agtaatcgcg cgccgcctcg tcgacgaaag
      721 cgaactcgtc ggcgagcagc tcttcgtcga agaggcttcc gctgtcgaag gcgtgctcgc
      781 gcagcatccg ctggcaccac gcatcgatcg tgagcacggc cgcgtcgtcc atcgattcgg
      841 cggccgtcga caaccgccgt gccgctgcgg cgcgcgcttc gccggatgcg tagtcgtcga
      901 tcaatgcgcg caggaaggcg tcgtcgctcg cgggctcgcg ctcgccgcga aagcaccgcg
      961 ccgcttcgac caggcgactg cggatgcgat cggaaagctc gcgcgtcgcc gccaccgtga
     1021 acgtcatgac gaggatctcg gcgggcacca acggccgcgt gaatgcggca ccgctctcgc
     1081 cgtggccgag aacgagccga agataaaggc cggcgatcgt ccaggtcttg ccggtgccgg
     1141 cgctcgcctc gatcaggcgg ctgccacaga gcggaacgcg caggggatcc agcgtctcag
     1201 acatgcggcg cctcctcggc acgcggcacc gcttccgctt cgcgctgcag cacctcgaca
     1261 cgggcagcga tccagtcgag catcggacgg tagatccgct cggccagcgc tttgaagccg
     1321 ccgtcggcat tgagcgcggc gaagtcggga aagcagcggc cgaggcatgg ctccgatcgc
     1381 tctccgtccc ggtcgaaggc accgtcgtac atcagttcgg cgttctcgcc gaggacgaag
     1441 gcgatggcag tgcgcgcagg caacggcagt ggctcttcca tgccgcgccg ccaggcagcc
     1501 aggagatgcc gcagggcctc ggccgcatcg ggctgatcga gcgtgcccag ccgaagcgtc
     1561 acgtcccgac cgacgtagac acccggcgca gcgcagccgc aggcgctgga aacgagcacg
     1621 cgcacccagg cgtcgatcag cttgtcggcg ctgggttgca cgccgtcgct gtccgcaagg
     1681 gccgagggac gcacgcgtaa ccaggcgtcg agtgttccat cggattgcag gccgtcgagc
     1741 cagtcgtcga caacgacccc gtcggcttcg aagtgcaggt gccgcggggg cgccggatcg
     1801 gcgaatcgac tctggaagcg acgccgctcc gagagcatcg gcgcagcgat gcccgccaac
     1861 tctgacccga gacggcttgc cgcctcgccg atcggaagtc ttcccgcacc ctggatgcgt
     1921 tgcaaccgcc gatcgagcga ctcgtcgctg ccttcacttg gccgacctgg cgattcgtcg
     1981 atgagatcgc acaacatggc gtagcgatcg aggccgtcga gcacgaacgt ttcgtcgtcg
     2041 tagttcgccg cttcctcgtc gtcgaaggcg acgccgagac gaacccggaa gaaggtcttg
     2101 accggattcc tgaggaaccg cgccaactcg tcgatcgtga gcggagcgtc gcgccgctcg
     2161 aaagccggca tcgatggcgc ggtcacgtcg ggctccgtac cccgacggtg cgccgatcgc
     2221 cactcgcgtg cgtaggtgaa gagcccgcgg ccttcgaaat agcggctgct gaacggctgc
     2281 agcgggtgct cggtcgtgcg cagcgcgacc gcgtcttccg ccgcgttgcc actccacccg
     2341 gcgcgcaggt agtcgcgcag ctgcgagacc agcaccgagg gcggctcgtc gccgttgtcg
     2401 cgggcgttcc ggccgctcca gctcacgtag agcacgcggc gggcggagag cagcgcctcc
     2461 agcatcaggt aacggtcgtc gtcacggcgc gagcggtcgc cggggcgctg ctggcccggc
     2521 agccccatca ggtcgaaatc gctgcgccgg ctctggcgcg ggtaggcgcc gtcgttcatg
     2581 ccgagcaggc acaccacctc gaacggcacc gcgcgcagcg gcatcaaggt gcagaacgtg
     2641 acgccgccgg caaggaagcg gcgcgctccg cccggcggat cgacggcgga aagccacgct
     2701 tcgcgaaaga ccgcgagcgg caccggctcg tcgaagcctg cgagctcgca ggcttccagc
     2761 cattcggcca acgccgactc cagcgcagcc agggtcagcc gctcgctctc gtcggccggc
     2821 tcgaccagcg cggcgagcag ctcacggccg cggcgcgccc actcgcgcgc ggtggctgcg
     2881 ctttggcact gcgcgcacca ttcgccgagg cgatcgacca gatcggcgag cgcgccgaca
     2941 agcgctgctt ccagaccgcc gatctcgtcg tatggctcca cgccggcgaa cgctgcgccg
     3001 ctcgcgtagc cgagcagcat gcggcgcagg ccgaagcgcc aggtgttctg cacgccgctc
     3061 gcttcgagcc cgagcccggc ccgctgcgcc gcgtcgaggc cccagcgcac gccggcgccc
     3121 tcgatccagc cggcgagcct gggcaggtcg gcgtcttcga ggccgaagcg ccgcgcgatc
     3181 gccggcactt cgagcaggtc tttcacttcc gatgcccgga agcgctgcgc tggcgcgcgc
     3241 agcagccatt cgagcgcgac gaccagcgga tcgttgccgc gattcctgat gtcgacgatc
     3301 ccgaacggaa tccgccgcgc gtcgcccggc gcgtgctggc cgaagacggc gcgaatggcg
     3361 ggggcgaagg tgtcgatgtc gggcaccatc acgacgatgt cgcgcggcct gagaggctgg
     3421 gtcgaccccg tatcagccag cagtgccagc agctgatcgt gcagcacctc gacctcgcgc
     3481 tgcgggttgt gcgccacatg gaagacgatc gagcggtcgc tcggcgccgc ggcccgcgca
     3541 gggtgctcgg cgagcggcac gaggtcgcgg atcatggcct gcacctgctc caacatgtgg
     3601 gcgccggcgc cttcttcgaa gagatcgatg cgcggcacgt cgaacaggtt tctcgaccgc
     3661 tccgcgtcgt cgaaggcgtc gagctggcgc atgaagtcgc gcccctgtcg
//
LOCUS       DASRWS010000048         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_881111, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000048 DASRWS010000000
VERSION     DASRWS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881111"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFF72_00845"
     CDS             complement(<1..449)
                     /locus_tag="VFF72_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV91728.1"
                     /translation="MPGDAAPVVAVDHVSVVYPAREHTVTALADVSLDIGEGEFISLI
                     GPSGCGKTTLLRVIADLEPVTAGSVRVNGVPAREARLGRAYGYVFQAPALFPWRTVLG
                     NCCLPLEIHGLDKARARAVAMTHLESVGLTGFEGKYPWQLSGGMQQRV"
     gene            complement(464..1774)
                     /gene="preA"
                     /locus_tag="VFF72_00850"
     CDS             complement(464..1774)
                     /gene="preA"
                     /locus_tag="VFF72_00850"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HZV91729.1"
                     /translation="MADLRSSFAGITSPNPFWLASAPPTDKEYNVVRAFEAGWGGVVW
                     KTLGEAGPPIVNVNGPRYAAIHSQDRRLIGLNNIELITDRPLETNLAEIARVKRAWPD
                     RALVVSIMVPCTEEAWKAILPQVEDTGCDGVELNFGCPHGMSERGMGSAVGQVPEYIE
                     MVARWCKHYTRLPVIVKLTPNVADIKPPALAAHRGGADAVSLINTVNSVMGVDLDALA
                     IDPHTGATGSHGGYCGPAVKPIALHMVAEIARTPATAAMPISGIGGITTWKDAAEFIA
                     LGAGNVQVCTAAMTYGFRIIDELTSGLAEYLDAKGLADVEALRGRAVPSLVDWKDLDL
                     NRVEKARIDQDLCIRCGRCHAVCEDTSHQAITKTKDGVRHFEVIDAECVGCNLCVSVC
                     PVPGCITMHALPAGVVDPRTGRVVDGGYANWTTHPNNPARVSAA"
     gene            complement(1793..>3072)
                     /locus_tag="VFF72_00855"
     CDS             complement(1793..>3072)
                     /locus_tag="VFF72_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV91730.1"
                     /translation="QVTRCNQCDDAPCVAACPTGIDIPSFIRRIGEDNLRGAAKAILD
                     ANVFGGICARVCPTEVLCEQACVRNTNEDKPVEIGLLQRRATDAYFASPGAPIFTRQA
                     PTGKRIAVVGAGPGGLSCAHRAAMLGHDVTVFEARPKPGGLNEYGLATYKTTGDFAQK
                     EIDWLLSIGGIEIRHGQSLGEQFTLDSLTAAYDAVFLGIGLGGVNALGLAAERTPGVG
                     NAVEFIAGLRQVSDYAALPVGRRVVVIGGGMTAIDAAVQSRLLGAEEVTLVYRRDEAR
                     MPASGHEREWAQKSGVTIRTWSVLDSIDGDAGHVEGATFATVHEVGGKLEGTGKRWRI
                     AADTVLKAIGQTLLLADPTLATLALREGRIAVDAEGRTSLPRVWAGGDCTWGGQDLTV
                     EAVEHGKIAAHSIDRALGMVRAGTPFARLRRAA"
BASE COUNT          426 a         1060 c         1106 g          480 t
ORIGIN      
        1 acgcgctgct gcatgccgcc cgagagctgc cacgggtatt tgccctcgaa gcccgtcagc
       61 ccgacgcttt cgaggtgggt catcgcgacg gcgcgcgcgc gcgccttgtc gaggccgtgg
      121 atctcgagcg gcaggcagca gttgccgagc accgtgcgcc agggaaagag cgccggcgcc
      181 tggaagacgt agccgtaggc gcgtcccagc cgcgcctcgc gcgccggcac gccattgacg
      241 cgaacgcttc ccgcggtcac cggctcgagg tcggcgatga cgcgcagcag cgtcgtcttg
      301 ccgcaacccg acgggccgat cagcgagatg aattcgcctt cgccgatgtc caggctcacg
      361 tcggcaagcg ccgtcaccgt gtgctcgcgc gccggataga cgaccgagac atggtcgacc
      421 gcgaccaccg gggcggcgtc ccccggcacg ggcagactcg acatcaggcg gcgctcacgc
      481 gggcggggtt gttcggatgc gtcgtccagt tggcgtagcc gccgtcgacg acgcgaccgg
      541 tccgcgggtc gacgacgcct gccggcagcg catgcatggt gatgcagccg ggcaccgggc
      601 agaccgagac gcacaggttg cagccgacgc attcggcgtc gatgacctcg aagtggcgga
      661 cgccgtcctt cgtcttcgtg atcgcctggt gcgaggtgtc ctcgcagacc gcgtggcagc
      721 ggccgcagcg gatgcacagg tcctggtcga tgcgcgcctt ctcgacccgg ttcaggtcga
      781 ggtccttcca gtcgaccagg ctcggcacgg cgcggccgcg cagcgcctcg acgtcagcga
      841 ggcccttcgc gtcgaggtat tcggccaggc cgctggtgag ctcgtcgatg atcctgaaac
      901 cgtaggtcat cgccgcggtg cacacctgca cgttgccggc cccgagggcg atgaactcgg
      961 ccgcatcctt ccatgtcgtg atgccgccga tgcccgagat cggcatcgcg gccgtcgccg
     1021 gcgtgcgcgc gatctcggcc accatgtgca gggcgatcgg cttgaccgcc ggcccgcagt
     1081 agccgccgtg cgatcctgtc gcgccggtgt gcggatcgat cgccagcgcg tcgaggtcga
     1141 cgcccatcac cgagttcacg gtgttgatga gcgacactgc gtcggcgccg ccgcgatgcg
     1201 cggccagcgc cggcggcttg atgtcggcga cgttgggcgt gagcttgacg atcaccggaa
     1261 gccgcgtgta gtgcttgcac cagcgcgcca ccatctcgat gtactcgggc acctggccga
     1321 ccgccgagcc catgccgcgc tcgctcatgc cgtgcgggca gccgaagttg agctcgacgc
     1381 cgtcgcagcc ggtgtcctcg acctggggca ggatcgcctt ccacgcctct tccgtgcacg
     1441 gcaccatgat cgagacgacg agcgcgcgat cgggccaggc gcgtttcacg cgggcgatct
     1501 cggccaggtt ggtctcgagc ggccggtcgg tgatgagctc gatgttgttg aggccgatca
     1561 ggcgccggtc ctgggaatgg atcgccgcgt agcgcggccc gttgacgttg acgatcggcg
     1621 gcccggcttc gccgagcgtc ttccacacga cgccgcccca gccggcctcg aaggcgcgca
     1681 cgacgttgta ttccttgtcg gtgggcggcg cggaggcgag ccagaacggg ttcgggctgg
     1741 tgatgcctgc gaagctgctg cgaagatcgg ccatggtcta gtcgttgtcg gatcaggcgg
     1801 cgcggcgcag ccgcgcgaaa ggtgtaccgg ctcgcaccat gccgagggcg cggtcgatcg
     1861 agtgcgcggc gatcttgccg tgctcgacgg cctcgaccgt gaggtcctgg ccgccccatg
     1921 tgcagtcgcc accggcccac acccggggca gagaagtgcg gccttcggcg tcgaccgcga
     1981 tccggccttc gcgcagtgcc agggtcgcca gcgtcgggtc ggcgagcagc agcgtctggc
     2041 cgatcgcctt gaggaccgtg tcggcggcga tccgccagcg cttgccggtg ccttcgagct
     2101 tgccgccgac ctcgtggacg gtggcgaagg tcgcgccttc gacgtggccg gcgtcgccgt
     2161 cgatcgaatc gaggacgctc caggtgcgga tcgtgacgcc gcttttctgc gcccactcgc
     2221 gctcgtggcc cgaggccggc atgcgcgcct cgtcgcggcg atagacgagg gtgacttcct
     2281 cggcgccgag caggcgcgac tggacggccg catcgatggc cgtcatgccg ccgccgatga
     2341 cgacgacccg ccgccccacc ggcagcgccg cgtagtcgct cacctggcgc aggccggcga
     2401 tgaattccac ggcattgccg acgcccggcg tccgttcggc cgcgaggccg agcgcgttga
     2461 cgccgcccag gccgatgccg aggaagaccg cgtcgtaggc ggcggtgagc gagtcgagcg
     2521 tgaactgctc gccgagcgac tggccgtgcc ggatctcgat gccgccgatc gagagcagcc
     2581 agtcgatttc cttctgcgcg aaatcgccgg tcgtcttgta ggtggcaaga ccatattcgt
     2641 tgagaccacc gggcttgggg cgcgcctcga acacggtcac gtcgtgcccg agcatcgctg
     2701 cgcgatgcgc gcacgacagg ccgcccggcc ccgcgcccac caccgcgatg cgcttgccgg
     2761 tcggcgcctg gcgcgtgaag atcggcgctc ctggagacgc gaagtaggcg tcggtggcgc
     2821 gccgctgcag gaggccgatc tcgaccggct tgtcctcgtt ggtgttgcgc acgcaggcct
     2881 gctcgcacag cacctccgtc gggcagacgc gcgcgcatat gccgccgaac acgttggcgt
     2941 cgaggatcgc cttggcggcg ccgcgcaggt tgtcctcgcc gatgcggcgg atgaacgacg
     3001 ggatgtcgat tccggtcggg caggcggcga cgcacggcgc gtcgtcgcac tggttgcagc
     3061 gggtgacctg ga
//
LOCUS       DASRWS010000049         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1460194, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000049 DASRWS010000000
VERSION     DASRWS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460194"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFF72_00860"
     CDS             <1..629
                     /locus_tag="VFF72_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV91731.1"
                     /translation="HELGFSGNVNDYYDPRNSYIHCLLETRRGIPITLALVYVELANQ
                     IGLDAHGVSFPGHFLARLRMPQGDIVIDPFTGQSMSREALDALLLPYRRQRSAEPGDE
                     LPLGAFLQAAKPREVIARMLRNLKEIFRNSQDPARLLGVAERLVILLPDAWEERRDRG
                     LVHAALGARLPAMVDLAGYLEHAPDAPDRPAISQRLAELGHDGRSRLH"
     gene            748..1419
                     /gene="hda"
                     /locus_tag="VFF72_00865"
     CDS             748..1419
                     /gene="hda"
                     /locus_tag="VFF72_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HZV91732.1"
                     /translation="MKQIPLAIGAEPARTFANFLPGPNAAAVAHLQALKPGAPPVYLW
                     GPAGSGKTHLLQSVVDSAQRHGALTAWFTPASPLPWQVPEHGDWVVFDDCAAFDAARQ
                     HAAFALFVDAVGRGAVVLAAAAVPPVDLRVREDLRTRLGWGHVFALQPLAEPEVRSAL
                     RREADRRGTFLSDEVMDFVLTRFARDLKHLMAQLDRLDAFSLATKRAITVPLLKQMLA
                     EEGGA"
     gene            1416..2084
                     /locus_tag="VFF72_00870"
     CDS             1416..2084
                     /locus_tag="VFF72_00870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZV91733.1"
                     /translation="MNLCLFDLDHTLLPIDSDHAWGEFMISLGWVDETEFRRGNDAFF
                     ARYVEGRLDIDAYVAFAVEPLRRRSDGELESAQRRFMQQVIDPAIRPAARTLVERHRR
                     QGDRIVLVTSTNEFVTAPIAAAFGIDTLIATRLEHGEDGRITGRIDGTPAFREGKVER
                     VGQWLARSGLSWADCGRITAYSDSLNDLPLLERASHPVATNPSPELAALAERRGWPII
                     RLFE"
     gene            2081..>3199
                     /locus_tag="VFF72_00875"
     CDS             2081..>3199
                     /locus_tag="VFF72_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZV91734.1"
                     /translation="MIKKFIEKILGKSASAAGKRVEVAKAVHGIDRQRVDERAVKVVQ
                     TLVDAGHEAYIVGGAVRDLLVGLKPKDFDVATDATPEQVKALFRRAFIIGRRFRIVHV
                     VFGRGRQERGRQAAGVGADAPGAGGRRGAVPWAPAAHEVIEVSTFRAVLDATAADQVA
                     GNEKTSKSELAGRSHVVDAEGRVLRDNVWGPQIEDAARRDFTINAMYYDPLREIVVDY
                     HGGIKDVKKKLLRMIGDPATRYREDPVRIIRAVRFAAKLGFEIEAKTRAPIKAMAALL
                     DNVPQSRTFDEMIKLLQTGHALASIVELKKLGLHRGVFPVLDVALDEAQRHDGREKFV
                     RAALADTDRRVAEGRPVAPSFMLAAMVWHDVLEGWKSRQ"
BASE COUNT          481 a         1157 c         1073 g          488 t
ORIGIN      
        1 tccacgagct cggcttcagc ggcaacgtca acgattacta cgacccgcgc aacagctaca
       61 tccattgcct gctcgagaca cgtcgcggca tcccgatcac gctggcgctg gtctatgtcg
      121 agctggcgaa ccagatcggc ctcgacgccc acggcgtctc gtttcccggc cacttcctgg
      181 cccgcttgcg catgccgcag ggcgacatcg tcatcgaccc tttcaccggg cagtcgatgt
      241 cgcgggaggc actcgatgcc ttgctgctgc cgtaccggcg gcagcgctcg gccgagcccg
      301 gcgacgagct gccgctgggc gctttcctgc aggcggccaa gccgcgcgag gtcatcgccc
      361 ggatgctgcg caacctgaag gagatcttcc gcaacagcca ggacccggcc cgacttctcg
      421 gcgtcgccga acggctggtc atcctcctgc ccgacgcgtg ggaggagcgg cgcgaccgcg
      481 gcctggtcca cgccgcgctc ggcgcgcgcc tgccggcgat ggtcgacctc gccggctacc
      541 tcgagcacgc gcccgacgca cccgaccgcc cggcgatcag ccagcggctg gccgagctcg
      601 gccacgacgg gcggtcgcgg ctgcactgag ccccgggggt cgaaggccgg caccgtccat
      661 cgggcatcat tggcggttgc ctgcaaccgc cgccccgtcc cgcatgccag cgcgctgccc
      721 tcccatgcct tcctgcccgt cgaacggatg aagcagattc cgctcgccat cggcgccgag
      781 ccggcgcgga ccttcgccaa tttcctgccc gggccgaatg cggcggcagt cgcccatctc
      841 caggccctca agcccggcgc tccgccggtc tatctctggg ggcccgcagg cagcggcaag
      901 acgcacctgc tgcaatcggt ggtcgattcg gcgcagcggc acggcgcgct gaccgcgtgg
      961 ttcacgccgg cttcgccgtt gccgtggcag gtgccggagc atggcgactg ggtggtcttc
     1021 gacgactgcg cggcgttcga tgctgcccgg cagcatgccg ctttcgcgct gttcgtcgac
     1081 gcggtcggcc gcggcgccgt cgtccttgcc gccgcggccg tgccgccggt cgacctgcgc
     1141 gtccgcgagg atctgcgaac ccgactcggc tggggccacg tgttcgccct gcagccgctc
     1201 gccgagcccg aggtgcggtc ggcgctgcga cgcgaggccg accggcgcgg aactttcctc
     1261 tcggacgagg tgatggactt cgtgctgacc cgctttgccc gcgatctcaa gcacctgatg
     1321 gcgcagctcg atcgcctcga cgccttttcc ctcgccacca agcgcgcgat cacggtgccg
     1381 ctgctcaagc agatgctcgc cgaggagggc ggcgcttgaa cctgtgcctg ttcgacctcg
     1441 atcacaccct gctgccgatc gattcggacc acgcctgggg cgagttcatg atctccctgg
     1501 ggtgggtcga cgagacggaa ttccggcgcg gcaacgatgc cttcttcgcg cgatacgtcg
     1561 aagggcgact cgacatcgac gcctatgtcg cgttcgcggt cgagccgctt cgccgccgca
     1621 gcgacggcga gctcgagtcg gcccagcgtc gtttcatgca gcaggtcatc gaccccgcga
     1681 tccgcccggc tgcccgcacg ctggtcgagc ggcatcgtcg gcagggcgac cgcatcgtcc
     1741 tggtgacgtc gaccaacgag ttcgtcaccg cgccgatcgc cgcggcattc ggaatcgaca
     1801 ccttgatcgc gacccgtctc gagcatggcg aagacggtcg catcaccggc cggatcgacg
     1861 gcacgccggc gtttcgggaa ggcaaggtcg agcgtgtcgg acaatggctg gcccgaagcg
     1921 gcttgtcgtg ggccgactgc gggcgcatca ccgcgtacag cgattcgctc aacgacctgc
     1981 cgttgctcga gcgcgccagc catcccgtcg ccaccaatcc ttcgcccgag ctcgcggcgc
     2041 tcgccgagcg gcgcggctgg cccatcatca ggctgttcga atgatcaaga aattcatcga
     2101 gaagatcctc ggcaagtcgg cgtcggccgc cggcaagcgc gtcgaggtgg ccaaggccgt
     2161 gcacggcatc gaccgccagc gggtcgacga gcgcgccgtc aaggtcgtgc agacgctggt
     2221 cgatgccggc cacgaggcct acatcgtcgg cggcgcggtg cgcgacctcc tggtcggcct
     2281 caagccgaag gacttcgacg tcgccaccga cgcgaccccc gagcaggtga aggcgctctt
     2341 tcgccgcgcc ttcatcatcg gccggcgatt ccgcatcgtt cacgtcgtct tcgggcgagg
     2401 ccgacaggag cgcggccgcc aggcggcagg cgtcggtgcc gacgcgccgg gggccggggg
     2461 ccgacgcggc gcggtcccgt gggcgccagc cgcccacgag gtgatcgagg tgtcgacttt
     2521 tcgcgcggtt ctcgacgcga ccgccgccga tcaagtcgcc ggcaacgaga agacctcgaa
     2581 gagcgaactg gccggcagga gccacgtcgt cgacgccgag ggtcgcgtcc ttcgcgacaa
     2641 cgtctggggc ccgcagatcg aggacgccgc gcgccgagac ttcacgatca acgccatgta
     2701 ctacgacccg ctgcgcgaga tcgtggtcga ctaccacggc ggcatcaagg acgtgaagaa
     2761 aaagctgctg cgaatgatcg gcgacccggc gacgcgctat cgcgaagacc cggtccgaat
     2821 catccgcgcc gttcgcttcg ctgccaagct gggcttcgag atcgaagcca agacccgggc
     2881 gccgatcaag gcgatggcgg cactgctcga caacgtgccg cagtcacgca ccttcgacga
     2941 gatgatcaag ctcctgcaga cgggccacgc gctggcctcg atcgtcgagc tgaagaagct
     3001 cggcctgcac cgcggcgtct tcccggtgct cgacgtcgcg ctcgacgagg cgcagcgcca
     3061 cgacggccgc gagaagttcg tccgcgcggc gctggccgat accgaccgtc gcgtcgccga
     3121 gggccgaccc gtggcaccga gcttcatgct cgcggcgatg gtctggcacg acgtgctcga
     3181 gggctggaag tcgcgacag
//
LOCUS       DASRWS010000050         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2611689, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000050 DASRWS010000000
VERSION     DASRWS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611689"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFF72_00880"
     CDS             <1..955
                     /locus_tag="VFF72_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV91735.1"
                     /translation="VLASALGVVSAAQAQAAWPAARPITLIVPFGAGGSVDVTARLVG
                     QKLGERLHQTVVIENITGAGGVIGVAKAVQAAPDGYTLVLGADSPIAIAKLVSPASVR
                     YDALKDLAPIGLVTTAPMIIVARPGLAANNLAEVLQLARAQPGKLSYGTSGVGTVLQL
                     AMEMLKEQAKVDIVHVPYRGGAQIVNDVIGNQLDLAVLVSITAVPHVRSKQVKGIAVT
                     DDKRLASLPDVPTVAETPGFKGFDVVAWTGLFAPAHTPPAIVEQLNRELGEVLRSDDV
                     RAKLAEQGAIAGSGSAAAFAAFVEHEQARYARIVQAAHIKQ"
     gene            complement(945..1235)
                     /locus_tag="VFF72_00885"
     CDS             complement(945..1235)
                     /locus_tag="VFF72_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91736.1"
                     /translation="MKSLAFVLAVMASGAAMAADGGAGATCAPHLSPLRARIYQKAVD
                     GPAVLREFLFIRRGILQLDVTETADWAAAVTRARAACMKNVAHAEPTSPVIV"
     gene            complement(1276..1548)
                     /locus_tag="VFF72_00890"
     CDS             complement(1276..1548)
                     /locus_tag="VFF72_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HZV91737.1"
                     /translation="MPRFVIEREIAGAGKLSAADRQGISATSCGVLRDLGPRIQWVES
                     FVTDDKIYCVYIAPDEALVREHARRGGFPANKVSQVRSRIDPTSAE"
     gene            complement(1593..1724)
                     /locus_tag="VFF72_00895"
     CDS             complement(1593..1724)
                     /locus_tag="VFF72_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91738.1"
                     /translation="MKTSNFARVTALAVAVLVTFGAIDLMAGYAYPAAPAVQLASAR"
     gene            complement(2070..>2247)
                     /locus_tag="VFF72_00900"
     CDS             complement(2070..>2247)
                     /locus_tag="VFF72_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV91739.1"
                     /translation="PAGSDKERALAAAVPVRRLGEAADVARAVRFFVDPQNGFVTGQV
                     LYVCGGTSVGSLTL"
BASE COUNT          344 a          751 c          816 g          336 t
ORIGIN      
        1 tgtcctggcg tctgcgctcg gcgtggtgtc tgcagcccag gcgcaggcgg cctggcccgc
       61 tgccagaccg atcaccctca tcgtgccgtt cggcgccggc ggcagcgtcg acgtcacggc
      121 ccgcctggtc gggcaaaagc tcggcgagcg gctgcaccag acggtggtga tcgagaacat
      181 caccggcgcc ggcggtgtca tcggcgtggc caaggccgtc caggccgcgc ccgatggcta
      241 caccctcgtg cttggtgccg acagcccgat cgcaatcgcc aagctcgtca gcccggcttc
      301 ggtcaggtac gacgcgctga aggatctcgc gccgatcggc ctggtgacga cggcgccgat
      361 gatcatcgtc gcgcgccccg gcctggccgc gaacaacctc gccgaagtgc tgcagctggc
      421 gcgcgcccag cccggcaagc tgagctacgg cacctcgggc gtcggcaccg tgctgcagct
      481 ggcgatggag atgctgaagg agcaggccaa ggtcgacatc gtgcacgtgc cgtatcgcgg
      541 cggcgcgcag atcgtcaacg acgtcatcgg caaccagctc gacctggcgg tcttggtgag
      601 catcacggcc gtgccgcacg tgcgcagcaa gcaggtcaag ggcattgccg tcaccgacga
      661 caagcggctc gcctcgctgc ccgacgtgcc gacggtggcc gagacgcccg gcttcaaggg
      721 cttcgacgtc gtcgcctgga ccggcctctt cgcgccggcg cacacaccgc cggcgatcgt
      781 cgagcagctg aaccgcgagc tcggcgaggt gctgcgttcc gacgacgtac gcgccaagct
      841 cgccgagcag ggcgcgatcg cgggcagcgg ctcggcggcc gcctttgccg cattcgtcga
      901 gcacgagcag gcgcgctacg cccgcatcgt gcaggccgct cacatcaaac aataacgggc
      961 gacgtcggct cggcgtgtgc gacgttcttc atgcacgcgg cacgtgccct ggttacggcg
     1021 gcggcccagt ctgccgtctc ggtgacgtcg agctgcagga tgccgcgccg tatgaacagg
     1081 aattcgcgca gcacggccgg gccgtcgacg gccttttgat agatgcgcgc ccgcagcggg
     1141 ctgagatggg gcgcgcaggt agcgccggcg ccgccgtcgg cggccatcgc ggcgccgctg
     1201 gccatgacgg ccagaacgaa tgccaatgac ttcatgtcgt ttctcctcgg ggttgctggg
     1261 gaaggaagcg ccgagctatt cggcgctggt gggatcgatg cgcgagcgca cctgcgacac
     1321 cttgttggcg ggaaagccgc cgcgtcgggc gtgctcccga acgagggctt cgtccggggc
     1381 gatgtagacg cagtagatct tgtcgtcggt gacgaagctc tcgacccact ggatccgggg
     1441 cccgaggtcg cgcagcacgc cgcacgaggt ggcggagatt ccctgccgat cggctgcaga
     1501 gagtttcccg gcgccggcga tctcgcgttc gatgacgaat ctgggcatgt cgatctcctg
     1561 gctgacctgg cagggggacc ggcggtggca ggtcaacgag ccgaggccag ctggacggcc
     1621 ggagccgccg ggtaggcgta gccggccatc aggtcgatgg cgccgaaggt gacgaggacg
     1681 gcgaccgcca gggcggtgac acgggcgaag ttcgaggttt tcatcgcagg ctccttcgag
     1741 gggtgcacca gggtgcgttg tcgatggaga ggactgtcgg ggctgcaccg cctttcagtc
     1801 ttgaagcgtc ctgaaatctc gcctgaaacg ttctgaaggc ggctccagct cagattcacg
     1861 gccgggggaa tgcggattca cggccaggga tctgcgccga cccggtcgag cagatcgccc
     1921 ttcgcgcact ggtggacgga ctgcgacagg cgctgcgggc tgccaggctc gccgcgctgg
     1981 cgcaggacgc gcggcgtcga gctacgaagg ggccgtgcgc ctggccctgc gcgaccgggg
     2041 cctgtttacg ccagatgggc ccgcaggccc tacagcgtca ggctgccgac gctggtgccg
     2101 ccacagacgt agagcacctg gccggtgacg aagccgtttt gcggatcgac gaagaagcgc
     2161 accgcacgcg ccacgtcggc ggcctcgccg agccggcgca ccggcaccgc ggcggcgagc
     2221 gcgcgctcct tgtcgctgcc ggcgggg
//
LOCUS       DASRWS010000051         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1174429, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000051 DASRWS010000000
VERSION     DASRWS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174429"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFF72_00905"
     CDS             complement(<1..716)
                     /locus_tag="VFF72_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV91740.1"
                     /translation="MIRDAGRLDRLLAQLRDFVRDECIPLEAQIDRDGEIPPALVERM
                     RTLGLFGHSIPEAYGGAGLTTEELALVNIEVSRAATVFRARFGGNTGIASESLVVDGT
                     EEQKRRWLPRLASGEVTGCFALTEPDAGSDATALATVAQRDGDDWLITGRKCYITNAP
                     LAGLFTVFARSDPDSRGAAGVSAFLVERGTPGLSTPPEYRKLGQHGSPVGEVLLERCR
                     VPASAIVGGEPGRGFKTAMKA"
     gene            complement(736..1578)
                     /locus_tag="VFF72_00910"
     CDS             complement(736..1578)
                     /locus_tag="VFF72_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZV91741.1"
                     /translation="MPAVSIRQAPGAGRPARAAPRADAEHQIDRQFSNNLARGLDVLR
                     AFTPDEPVLGNRELADRTGLPKPTISRLTYTLTLLGYLAYVERLQKYRLGAGVLSMGY
                     PLLASLQVRQVARPVMERIARETGCTVNLGMRDRTQVVYVDTCRVDHGNLYQPDIGSS
                     RPLLLTSIGRAIIVACAADARAAIVNRIKVQEPGQYAAGAAFWEADWRAFRERGFCLS
                     RGDWRKEVHAVAVPIRLPQREEPVALNCTTSAHRLARDALQRKVAPILVEGVRAIEAA
                     CGVV"
     gene            1777..>2780
                     /locus_tag="VFF72_00915"
     CDS             1777..>2780
                     /locus_tag="VFF72_00915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZV91742.1"
                     /translation="MSSELPPSSAAQGPLAGVRILDMATVLAAPFGATLCADQGADVV
                     KLELPDGSDALRFLQPVKDGHGLWFKVANRGKRGISLDVRKPEGRRLFLQLLPQFDVL
                     VENFRAGTLDKWGLDAATLFAANPRLVVLRLTGFGQTGPYRSRPGFARIFEAMSGFTN
                     LTGEAGGTPLHSNFPVGDAVAGVFAAFAIACEIARLRADPRASGVEVDLSATEALFRL
                     LDPLPVEHEQLGFVRGPAGNRASYTAPSSLYRSADGAHFSLVASSDSIFARLCHAIDR
                     ADWIDDPRFATNPGRVRHLVELDESLAAWFAARPYDAIATTLEAAGVPFSKVYSIADI
                     "
BASE COUNT          409 a         1005 c          949 g          417 t
ORIGIN      
        1 gccttcatcg ccgtcttgaa gccgcggccc ggctcaccgc cgacgatcgc cgacgccggc
       61 accctgcacc gctcgagcag cacctcgccg accggagagc cgtgctggcc gagcttcctg
      121 tactccggcg gcgtcgaaag gcccggtgtg ccgcgttcga cgagaaacgc ggagactccg
      181 gcggcgccgc gcgagtccgg gtcgctgcgc gcgaagaccg tgaagaggcc cgccagcggc
      241 gcgttcgtga tgtagcactt gcggccggtg atgagccagt cgtcgccgtc gcgctgggcc
      301 accgtcgcca gcgccgtggc gtcggagccg gcatcgggct cggtcagcgc gaagcagccc
      361 gtgacctcgc ccgaggccag gcgcggcagc cagcgccgct tctgctcttc ggtgccgtcg
      421 acgacgagcg actccgaagc gatgccggta ttgccgccga agcgggcccg gaacaccgtc
      481 gcggcacgcg agacctcgat gttgacgagc gcgagctcct cggtcgtcag gcccgcgccg
      541 ccataggcct cgggaatgct gtggccgaaa agaccgagcg tgcgcatccg ctcgacgagg
      601 gccggcggaa tctcgccatc gcggtcgatt tgtgcctcga gcgggatgca ctcgtcgcgc
      661 acgaaatcgc gcagctgcgc gagcaatcga tcgagtcgtc cggcgtcacg aatcacgttg
      721 cgtatgtcct tctcttcaga cgacgccgca ggcagcctcg atcgcccgca caccctcgac
      781 gaggatcggc gcgaccttgc gctgaagcgc gtcccgcgcg aggcgatgcg ccgacgtcgt
      841 gcagttcagc gccaccggct cctcgcgctg cggcagccgg atcggcacgg ccaccgcgtg
      901 cacttccttg cgccagtcgc cgcggctcag gcagaagccc cgctcgcgaa aggcgcgcca
      961 gtcggcctcc cagaaggccg cgccggcggc gtactgcccg ggctcctgga ccttgatgcg
     1021 attgacgatc gcggcgcgcg cgtcggccgc gcaggcgacg atgatcgccc ggccgatcga
     1081 ggtgagcagc aggggccgcg agctgccgat gtcgggctgg tagaggttgc cgtggtcgac
     1141 ccggcaggtg tcgacgtaga cgacctgggt gcgatctcgc atcccgaggt tcacggtgca
     1201 gccggtctcg cgggcgatcc gctccatcac cggacgcgcg acctggcgca cctgcaggct
     1261 cgcgagcagc gggtagccca tcgacaggac gccggcaccg agccggtatt tctgcaggcg
     1321 ctcgacgtag gccaggtagc cgagcagcgt caaggtgtag gtcaggcgcg aaatcgtcgg
     1381 cttgggcagc ccggtgcgat cggcgagctc gcgattgccg agcacgggtt cgtccggcgt
     1441 gaacgcacgc agcacgtcga gtccgcgcgc caggttgttc gagaactggc gatcgatctg
     1501 atgctcggcg tcggcgcgcg gcgccgcacg cgcagggcgg ccagctccag gggcttggcg
     1561 gatcgagacg gcaggcatcg aggtatcgat cgaagaggct tcaaggcggc ggcgagacgt
     1621 gcctcggcga ggcgtgaagc gcgcgctgtc cgacatgttc ggcaatcggc accgaggcgt
     1681 caacgaatcg aatcccgagt ttcgcagagc gaaatgaccg gtagcgacgc cggtaccacg
     1741 acgtcgacaa tgccgtcccg cctgccgtcg ccgccgatgt cctccgagct cccgccctct
     1801 tccgccgcgc aaggtccgct cgccggcgtc cgcatcctcg acatggccac cgtgctcgcg
     1861 gcaccgttcg gcgcgacgct ctgcgccgac cagggcgccg atgtcgtcaa gctcgagctg
     1921 cccgacggca gcgacgcgct gcgcttcctt cagcccgtga aggacggcca cggcctgtgg
     1981 ttcaaggtcg ccaaccgcgg caagcgcggc atcagcctcg acgtgcgcaa gcccgaaggg
     2041 cggcgcctct tcctgcagtt gctgccgcag ttcgacgtgc tggtcgagaa ctttcgcgcc
     2101 ggcacgctcg acaaatgggg cctcgacgcg gcgacgctct tcgccgccaa cccgcgcctc
     2161 gtcgtgttgc gcctgaccgg cttcggacag accgggccgt atcggtcgcg ccccggcttc
     2221 gcccgcatct tcgaggcgat gagcggcttc accaacctga ccggcgaagc cggcggcacg
     2281 ccgctgcaca gcaactttcc ggtcggcgac gccgtcgccg gcgtcttcgc ggcgttcgcg
     2341 atcgcctgcg aaatcgcccg gctgcgcgcg gatccccggg cctcgggcgt cgaggtcgac
     2401 ctgtcggcga ccgaggcgct gtttcgcctg ctcgatccgc tgcccgtcga gcacgagcag
     2461 ctcggcttcg tgcgcggccc ggccggcaac cgggcgagct acaccgcgcc gtccagcctc
     2521 taccgcagtg ccgacggcgc ccacttctcg ctcgtcgcat cgtcggattc gatcttcgcg
     2581 cggctctgcc atgcgatcga ccgcgccgac tggatcgacg acccgcgctt cgcgaccaac
     2641 cctgggcgcg tgcgtcatct cgtcgagctc gacgaaagcc ttgccgcctg gttcgccgca
     2701 cgaccctacg acgcgatcgc aaccacgctc gaagctgccg gcgttccgtt ctcgaaggtg
     2761 tactcgatcg ccgatatcga
//
LOCUS       DASRWS010000052         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1751393, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000052 DASRWS010000000
VERSION     DASRWS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751393"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1162
                     /locus_tag="VFF72_00920"
     CDS             170..1162
                     /locus_tag="VFF72_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZV91743.1"
                     /translation="MTDTASIPSEVATLMERVLYEVKRVVVGQDHFLERVMVAMLAQG
                     HLLVEGVPGLAKTLTVKTLAQTLRGTFKRIQFTPDLVPADLVGTRIFNPRTGEFSTTL
                     GPVFTHLLLADEINRAPAKVQSALLEVMQERQVTIAGETHKVPEPFLVMATQNPIETE
                     GTYPLPEAQVDRFMMKVLVDYPTEDEEFVIVQRVTGEPVSMAPIASMSQLAELQQECR
                     KVYVDPSLIQYAVKLVSATRRPERYGLGDVARYLTFGASPRATIGLVEGARALAFMRG
                     RAYALPEDMTGLVGDVFRHRLVLSYEALAEGLDADTLVDRVMRRIPVPDKLLEK"
     gene            1162..2199
                     /locus_tag="VFF72_00925"
     CDS             1162..2199
                     /locus_tag="VFF72_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZV91744.1"
                     /translation="MASSPSANASPRAGAPGRGQASPRDPGEAVDGAFESGERLLKRL
                     EWTVLRRLDGLLQGDHRTLMRGTGLDLADLREYQHHDDVRHIDWNVTARLQQPYVREF
                     TEDRELSAWFLVDLSASVDFGSDRFTKRRIAREFVGALARMLTRRGNRIGALLYGTEV
                     DTALPARGGRRHVLELLQRMASRPAEAAPGATDLRVLLDSALRAVKRRSTLFVVSDFI
                     SPPGWEKSLGQLARRHEVTAVRLFDPLEMDLPDIGLVTLRDAETGEQLVVDTHSAGFR
                     KRFKAAAERREQALREALGRAGVDTLELATDDDLASSILRFAELRKQRSRLANWSGAR
                     FGAPSHVRPAA"
     gene            2207..>3045
                     /locus_tag="VFF72_00930"
     CDS             2207..>3045
                     /locus_tag="VFF72_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZV91745.1"
                     /translation="MASPHPFLTFLWPNMLWAMLAVPVLVVVYLFLLTRRRKSTLRYG
                     NLALVKEALGRGGAWRRHVPPLLMLAAISALLFASSRPAALITLPSAQETIVLAMDVS
                     GSMRAADVLPSRLVAAQEAAKTFVSELPRNVKIAVVSFAGTAAVVQAPTLSRDDVYAA
                     IDRFQLQRGTAIGSGIVLSLATLFPEAGIDLSQITGEREMPPGPNDAPRPEFTPVPPG
                     SYPSAAIILLTDGQRTTGPDSLDAAKMAADRGIRVYTVGIGTREGETIQFEGWSMRVR
                     LDE"
BASE COUNT          458 a         1148 c          985 g          454 t
ORIGIN      
        1 ccatgccgat cgaaaatgcg gccgcggccg cgggcatgcc gccgttctga gccggcgagc
       61 gccgctcgac tgccgctcgt tcgcccgatt cgctcgcccc cgaagcggac gattgcgcca
      121 gatcccacag accgcccccc gcgccttccc accgagatcg acgaccccca tgaccgacac
      181 cgccagcatc ccgagcgaag ttgccacgct gatggagcgc gtcctctacg aggtcaagcg
      241 ggtcgtcgtc ggccaggatc atttcctcga gcgcgtgatg gtggcgatgc tggcgcaggg
      301 ccaccttctc gtcgagggcg tgccgggcct ggccaagacg ctcaccgtca agacgctggc
      361 gcagaccctg cgcggcacct tcaagcgaat ccagttcacg cccgacctgg tgccggccga
      421 cctcgtcggc acgcggatct tcaatccgcg caccggcgag ttctcgacga cgctcggtcc
      481 cgtcttcacc cacctgctgc tcgccgacga gatcaaccgc gcgcctgcca aggtgcagag
      541 cgcgctgctc gaggtgatgc aggagcgcca ggtgacgatc gccggcgaga cgcacaaggt
      601 gcccgagccc tttctcgtca tggcgacgca gaacccgatc gagaccgaag gcacgtatcc
      661 gctgcccgag gcgcaggtcg accgcttcat gatgaaggtc ctcgtcgact atcccaccga
      721 agacgaggag ttcgtcatcg tccagcgggt caccggcgag ccggtgtcga tggcgccgat
      781 cgccagcatg agccagctcg ccgagctgca gcaggaatgc cgcaaggtct acgtcgatcc
      841 gtcgctgatc cagtacgcgg tcaagctggt cagcgcgacg cgcaggcccg agcgctacgg
      901 actgggcgac gttgccaggt acctgacgtt cggcgccagc ccgcgggcga cgatcggcct
      961 ggtcgaaggt gcccgcgccc tggccttcat gcgcggtcgc gcctacgcgc ttcccgagga
     1021 catgaccggc ctcgtcggcg acgtcttccg ccaccggctg gtcctttcgt acgaggcgct
     1081 cgccgaaggc ctcgacgccg acaccctcgt cgatcgggtg atgcgccgca tcccggtccc
     1141 cgacaagctg ctggaaaaat gatggcgtcg tcgccttccg cgaacgcatc tccgcgcgcc
     1201 ggcgctccgg gccgcggcca ggcctcgcct cgcgacccgg gcgaggcagt cgacggcgca
     1261 ttcgaaagcg gcgaacgcct tctcaagcgc ctcgagtgga ccgtgctgcg ccgactcgac
     1321 ggcctgctcc agggcgatca ccgcacgctg atgcgcggca ccgggctcga cctggccgac
     1381 cttcgcgagt accagcacca cgatgacgtg cgccacatcg actggaacgt cacggcccgg
     1441 ctgcagcagc cctatgtgcg cgagttcacc gaagaccgcg agctctcggc gtggttcctg
     1501 gtcgatctgt cggcgagcgt cgatttcggc tccgaccgct tcaccaagcg gcggatcgcc
     1561 cgcgagttcg tcggcgcgct ggcgcgcatg ctgacccgcc gcggcaaccg catcggcgcc
     1621 ctgctctacg gcaccgaggt cgacaccgcg ctgccggcac gcggcggccg ccggcacgtc
     1681 ctcgagcttc tccagcgcat ggcatcccgg ccggccgagg cggcgcccgg cgcgaccgat
     1741 ctgcgggtcc tgctcgactc ggccctgcgc gcggtgaaac gacgctcgac cctcttcgtg
     1801 gtctccgact tcatcagccc gccgggctgg gaaaaatcgc tcgggcaact ggcccgacgc
     1861 cacgaggtca cggccgtccg cctcttcgac ccgctcgaga tggatctgcc cgacatcggc
     1921 ctggtcaccc tgcgcgacgc cgagaccggc gagcagctcg tcgtcgacac gcacagtgcc
     1981 ggctttcgca agcgcttcaa ggccgccgcc gaacggcgcg aacaggcgtt gcgcgaagcg
     2041 ctcggccggg ccggcgtcga caccctcgag ctcgccaccg acgacgacct ggccagctcg
     2101 atccttcgct tcgccgagct gagaaagcag cgcagccgcc tcgccaactg gagcggcgca
     2161 cgcttcggcg cgccgtcgca cgtccgcccg gccgcgtgag gacgagatgg ccagcccgca
     2221 tcccttcctg accttcctct ggccgaacat gctgtgggcg atgctcgccg tgcccgtgct
     2281 ggtcgtcgtc tatctcttcc tcctcacgcg gcgcaggaag tcgaccctgc gctacggcaa
     2341 cctggccctc gtcaaggagg cgctcgggcg aggcggcgca tggcggcgcc acgtgccgcc
     2401 gctcctgatg ctggcggcga tcagcgcgct gctcttcgcc tcgtcgcgtc ccgctgccct
     2461 catcacgctg ccgtcggcac aggagacgat cgtcctggcg atggatgtct cgggcagcat
     2521 gcgcgctgcc gacgtcctgc cgagccgcct cgtcgcggcc caggaagcag cgaagacctt
     2581 cgtctccgag ctgcctagga acgtcaagat cgccgtcgtc tcgttcgccg gcaccgccgc
     2641 ggtggtgcag gccccgacac tgtcgcgcga cgacgtctac gcggcgatcg atcgcttcca
     2701 gctgcagcgt ggcaccgcca tcggcagcgg catcgtcctg tcgctggcga cgctgttccc
     2761 cgaggccggc atcgacctgt cgcagatcac cggcgagcgc gagatgccgc ccggcccgaa
     2821 cgacgcgccg cggccggaat tcacgccggt gccaccgggg tcgtacccgt cggcggcgat
     2881 catcctgctc accgacggcc agcgcaccac cgggcccgat tccctggacg ccgccaagat
     2941 ggccgccgac cgcggcattc gcgtctacac cgtcggcatc ggcacccgcg aaggcgagac
     3001 gatccagttc gaaggctggt cgatgcgcgt gcggctcgac gagga
//
LOCUS       DASRWS010000053         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3761183, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000053 DASRWS010000000
VERSION     DASRWS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761183"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VFF72_00935"
     CDS             complement(<1..1819)
                     /locus_tag="VFF72_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZV91746.1"
                     /translation="MSSGRGAQGGPLRFGRFELQPTERRLLDDGRPVQIGSRAFDLLT
                     TLALRPGRLITKGELLDQVWPGLVVEESNLHTQMSSLRRVLGNEVIATVPGRGYRFAA
                     VAEATAAPAPPPATVAHVSTAALPMVGGRLFGRDDDQARLAALLEAPGCVTLVGAGGV
                     GKTRLALAAAAAWTGRRFWVDLSPLTEGVQVAGALARALGLPLADGDIGTPLVDALAG
                     GPAWVALDNAEHLVDATAALAKQLLAGAPELRLLVTSQLPLSLAGERVLRIEPLPLEG
                     GEGDGDGALALLTERITAADHRVRIGPDAVPLLREIVRQLDGLPLALEMAAARVPLLG
                     LKGVHDELAQRFALLTTGHRQSPARHRSLHGALEWSHGLLDSDERRLFAALSVFAGGF
                     TLELCVALMQDAATSRWAVIDRLAALVDRSLVAVGHDDPPRYRLLETMRAYALEQLAA
                     AGAEAAFRRRHAEVVLDHFRTHAPSEAGKALCRPELDNAREAIAWSSDHALPVAAALT
                     IHASQATTFSPWRVQSHAWMLALEPRMRSPEGEAMEVAARIVWWNDFARSSIVNLSAR
                     AREIARDARALARSHGDTRAQLVATVAVVRSLREPGPEQS"
     gene            complement(1934..>2220)
                     /locus_tag="VFF72_00940"
     CDS             complement(1934..>2220)
                     /locus_tag="VFF72_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91747.1"
                     /translation="MTELANAARRAGLCRLFGDVLQGNERMAAFMRRQGFDRACDDGP
                     DAGTGVQRWERPLRTPTSSARTGHAGPGTWHLPSLSGLLGQLAVRVAERL"
BASE COUNT          324 a          816 c          796 g          284 t
ORIGIN      
        1 cggactgctc ggggccgggc tcgcgcagcg agcggacgac ggcgaccgtg gccacgagtt
       61 gtgctcgcgt gtcgccatgg ctacgggcca acgcccgcgc gtcgcgcgcg atctcgcggg
      121 cgcgggcgct caggttgacg atcgaggacc gcgcgaagtc gttccaccag acgattcgcg
      181 ccgccacctc catcgcttcg ccctcggggg aacgcatccg cggctcgagc gccagcatcc
      241 acgcatgact ctgcactcgc cagggcgaga aagtcgtggc ctgcgatgcg tggatggtca
      301 gtgccgccgc caccggcaac gcatggtcgc tcgaccaggc gatggcttcg cgcgcgttgt
      361 cgagctcggg gcggcagagt gcctttccgg cctctgatgg cgcatgggtt cgaaaatggt
      421 cgaggacgac ctcggcatgg cgacgccgaa aggcggcctc ggcgccggcc gcggcgagtt
      481 gctcgagcgc gtaggcgcgc atcgtctcca gcaaccggta gcgcggtggg tcgtcgtggc
      541 cgacagcgac gagcgagcga tcgaccagcg ccgcgaggcg gtcgatcacc gcccagcgcg
      601 aggtggcggc atcctgcatg agcgccacgc acagctcgag cgtgaagccg cccgcgaaca
      661 cgctcagcgc ggcgaagagg cggcgctcgt cgctgtcgag caggccgtgg ctccattcga
      721 gcgcgccgtg cagcgagcgg tgacgggccg gtgactggcg gtgcccggtg gtcagcaagg
      781 cgaaacgctg cgcgagctcg tcgtgcaccc ctttgagccc gagcagcggc acccgcgccg
      841 cggccatttc cagcgccagt ggcaggccgt cgagctggcg cacgatctcg cgcagcagcg
      901 gcacggcgtc ggggccgatg cgcacgcggt ggtcggcggc ggtgatccgt tcggtcagca
      961 gggcgagcgc gccgtcgccg tctccttcgc cgccttccag gggcaacggc tcgattcgca
     1021 acacgcgctc gcccgccagc gacaacggca gctggctcgt gacgagcagg cgcaactccg
     1081 gcgcgccggc gagcagctgc ttcgccagcg cggccgtcgc atcgaccagg tgctcggcgt
     1141 tgtcgagcgc cacccatgcc gggccgccgg cgagtgcatc gaccagcggc gtgccgatgt
     1201 cgccatcggc aagcggcagc ccgagcgcgc gggccagcgc gcctgccacc tggacgcctt
     1261 cggtcagcgg cgacaggtcg acccagaacc gccgaccggt ccacgcggcg gcagccgcca
     1321 gcgccagccg tgtcttgccg acacccccgg cgccgaccag cgtgacgcag cccggcgctt
     1381 ccagcagggc cgccagtcgc gcctggtcat cgtcacggcc gaacagtcgt ccgccaacca
     1441 tcggcagggc cgcggtcgac acatgcgcga cggtcgcggg cggcggcgcc ggcgctgccg
     1501 tggcttcggc caccgccgcg aagcgatacc cgcgccccgg caccgtggcg atgacctcgt
     1561 tgccgagcac gcgccgcagc gagctcatct gcgtatgcag gttcgattcc tcgacgacca
     1621 ggccgggcca gacctggtcg aggagctcgc ccttcgtgat caggcgaccc ggtcgcaggg
     1681 ccagtgtcgt caggaggtcg aaggcgcgcg aaccgatctg cacggggcgg ccatcgtcga
     1741 gcaggcgccg ctcggtcggc tgcagctcga agcgcccgaa gcgaagcggc ccgccttgcg
     1801 cgccgcggcc gctggacacg ctcgaaactt ccggcacagg ctgactctcc agcgccacgc
     1861 tgccgccgcg cggaccgaac gggcgaaacc aagcttaccg cgtcgcgtcg tcgggtcgtg
     1921 gccgatgcat gcttcacagc cgctcggcga cgcgcaccgc cagttgcccg agcaatccgg
     1981 agagcgatgg caaatgccat gtccccggcc cggcgtgtcc ggtccgcgcc gatgaagtcg
     2041 gcgtccgcag cgggcgctcc cagcgctgca cgcccgtccc ggcgtcgggc ccatcgtcgc
     2101 aggccctgtc gaaaccctgg cgacgcatga acgcggccat gcgctcgttg ccttggagaa
     2161 cgtcgccgaa caggcggcag aggccggccc gacgcgccgc gttcgccagc tcggtcatca
//
LOCUS       DASRWS010000054         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1175103, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000054 DASRWS010000000
VERSION     DASRWS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175103"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VFF72_00945"
     CDS             <1..1016
                     /locus_tag="VFF72_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HZV91748.1"
                     /translation="VIGTITLTGVAQDFTQFLVSFSHDALWLALIVTAAAGIVLGMGM
                     PTTASYIVVSTLMAPVVVELGAQSGLVVPLVAVHMFVFYFGLMADVTPPVGLASFAAA
                     AISRADPIRTGITAFWYSIRTGILPFMFIFNPQLLMIGITSWWQLVLVIASAITAMLV
                     FAAATQQWFVTRSRWWESVALLLVTFTFLRPGFWLDMALPRFDVQPGQTIIERATAAA
                     PGSGLRVRIAGTTLEGKDVKKTVLLPIGDPAPGQKRLAEAGLRVMTLPTGTQVMSVGL
                     KSQAAKAGFEQGFMVTGIETERPRMAKEWLFVPALLLLGVIFLLQRRRLAKERRVPGV
                     ALA"
     gene            complement(1123..1207)
                     /locus_tag="VFF72_00950"
     tRNA            complement(1123..1207)
                     /locus_tag="VFF72_00950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Leu,seq:caa)
     gene            1299..1892
                     /locus_tag="VFF72_00955"
     CDS             1299..1892
                     /locus_tag="VFF72_00955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZV91749.1"
                     /translation="MPPLLPATQALLLANVAIFFLERLLGGGFVSTFALWPIGSGEFW
                     PWQIGTYAFLHGSFEHLFFNMLGLWMFGSELEQVWGRRRYLEFYAASVVAAALTQLLV
                     NYVLGNGAPTIGASGGLFGLLLAFAMIFPNRIILLFFVIPMKAKYLVLLYGLIELYQG
                     AYVMNTGVAHFAHLGGMLGGLLMIRYWRGQVPFGRRR"
     gene            complement(1915..2814)
                     /locus_tag="VFF72_00960"
     CDS             complement(1915..2814)
                     /locus_tag="VFF72_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV91750.1"
                     /translation="MKALLLTEPMPAAPFMAAIGAQVPELELLEYRPALGDDALADVD
                     IAIGWRLPEALAARLPRLAWICSIAAGVEKLLASGLAPSVVVSRIVDPAQAAGIAQFV
                     AAMVLRHARSLELYEAQQRARVWRREPTPMASPRVAVLGTGAMGGAIVDWLGRVGMRV
                     RGWNRRSAQTLAETLANADVVVCALPLTKDTERILDARAFAAMPAGSYLVNVARGAHV
                     VEADLIAAVRSGHLAGAALDVQCREPMAADDPLWSVPGITITPHIAAQSSTDAIVRQF
                     VDALRAWQRGEAIPNAIDRSRGY"
BASE COUNT          403 a         1044 c          993 g          487 t
ORIGIN      
        1 tcgtcatcgg cacgatcacg ctgacgggcg tcgcccagga cttcacgcaa ttcctcgtct
       61 cgttctcgca cgacgcgctc tggctcgcgc tgatcgtcac cgccgcggcc gggatcgtcc
      121 tcggcatggg catgccgacg acagccagct acatcgtcgt ctcgacgctg atggcgcccg
      181 tcgtcgtcga gctcggcgcg cagtcgggcc tcgtcgtgcc gctggtcgcc gtgcacatgt
      241 tcgtcttcta cttcggcctg atggccgacg tgacgccgcc ggtgggactg gcgtcgttcg
      301 ccgccgcagc gatctcgcgg gccgacccga tccgcaccgg catcaccgcg ttctggtaca
      361 gcatccgcac cgggatcctg ccgttcatgt tcatcttcaa cccgcagctg ctgatgatcg
      421 gcatcaccag ctggtggcag ctcgttctcg tcatcgcgtc cgccatcacg gcgatgctcg
      481 tcttcgccgc cgcgacgcag cagtggttcg tcacgcgcag ccgctggtgg gagtcggtcg
      541 ccctgctcct cgtcaccttc acgttcctgc gtccgggctt ctggctcgac atggcgctgc
      601 ctcgcttcga cgtgcagccc gggcagacga tcatcgagcg ggcaaccgcc gccgcgccgg
      661 ggtcgggcct gcgcgttcgc atcgccggaa cgaccctcga aggcaaggac gtgaagaaga
      721 ccgtgctgct gccgatcggc gatccggcgc cgggccagaa gcgtcttgcc gaagcggggc
      781 ttcgcgtgat gaccctgccg accggcacgc aggtgatgtc cgtcggcctc aagagccagg
      841 ccgcgaaggc cggcttcgag cagggcttca tggtcaccgg catcgagacc gaacgacccc
      901 gcatggccaa ggagtggctg ttcgtgccgg cactgctcct gctcggcgtg atcttcctgc
      961 tgcagcggcg acgtctggcg aaggagcggc gcgtgcccgg cgtggcgctg gcctgacgtc
     1021 ggcgccggca cagcggtcgc gaacggtcga aagactgttc gcgtcgagcc gagggttcag
     1081 actgtcgcag ggtgctcgcc tcccgagctt cggaacctgc tctggtgcct cgggtcggaa
     1141 tcgaaccgac actccttgcg gaacgggatt ttgagtcccg cgcgtctacc aatttcgcca
     1201 ccgaggccgc ggtgcggaca gggccgcgag tttgccacac cggccggctc ggccgatgcc
     1261 ccgtagactc cgcagcttcc gtcgttgccc gatcccgaat gccgccgctt cttcccgcca
     1321 cgcaggccct gctgctcgcc aacgtcgcca tcttttttct cgagcggctg ctcggcggcg
     1381 gcttcgtctc gaccttcgcg ctctggccga tcggcagcgg cgaattctgg ccctggcaga
     1441 tcggcaccta cgccttcctg cacggcagct tcgagcacct gttcttcaac atgctcggcc
     1501 tgtggatgtt cggctcggag ctcgagcagg tctggggccg caggcgctac ctcgagttct
     1561 acgcggccag cgtcgtcgcc gccgccctca cgcagctgct cgtcaactac gtcctcggca
     1621 acggcgcacc gacgatcggt gcgtcgggcg ggttgttcgg gctgctgctg gccttcgcga
     1681 tgatctttcc gaaccggatc atcctgctct tcttcgtcat tccgatgaag gcgaagtacc
     1741 tcgtcctcct gtatggcctg atcgagctgt accagggcgc ctacgtgatg aacaccggcg
     1801 ttgcccactt cgcccacctg ggtggcatgc tcggcggcct gctgatgatc cgctactggc
     1861 ggggccaggt gcccttcggc cgacgccgct gacgccgctg cggcgacgac gcggtcagta
     1921 gccgcgcgag cggtcgatgg cgttcgggat cgcttcgccg cgctgccatg cacgaagcgc
     1981 gtcgacgaac tggcggacga tggcgtcggt cgacgactgc gcggcgatgt gcggcgtgat
     2041 cgtgatgccg ggaaccgacc agagcgggtc gtcggccgcc atcggttcgc ggcactgcac
     2101 gtcgagcgcc gccccggcga gatgccccga gcgcacggcc gcgatgaggt cggcttcgac
     2161 gacatgcgcg cctcgcgcga cattgacgag atagctgccc gcgggcatcg cagcgaaggc
     2221 gcgggcgtcg aggatccgct ccgtgtcctt cgtcaacggc agggcgcaga cgacgacgtc
     2281 ggcattggcc agcgtctcgg caagggtctg cgccgaacgg cggttccagc cgcgcacgcg
     2341 catgccgacg cggcccagcc agtcgacgat cgcgccgccc atcgcacccg tgccgagcac
     2401 ggcgacgcgc ggcgacgcca tcggcgtcgg ctcgcgtcgc cagacgcggg cccgctgctg
     2461 cgcctcgtag agctcgagcg atcgcgcatg gcgcagcacc atggcggcga cgaactgggc
     2521 gatgcccgcg gcctgcgccg gatcgacgat ccgcgagacg acgacgctcg gggccagccc
     2581 gctcgccagc agcttctcga cgccggcggc gatcgagcag atccaggcca gccgcggcag
     2641 ccgcgccgcc aacgcctcgg gcaggcgcca gccgatcgcg atgtcgacgt cggcgagcgc
     2701 gtcgtcgccc agggccggac ggtactcgag caactcgagc tcgggcacct gcgcgccgat
     2761 ggccgccatg aaaggcgctg ccggcatcgg ctcggtgagc aggagggcct tcacgaggcg
     2821 aggaagttgc cgcagttcga gaaacgctgc gtcgcctcgg catccgggcc gcgcgcgacg
     2881 ccggccgggt cgtcgccggc ggcgacccga gcgagttgct cgcgcag
//
LOCUS       DASRWS010000055         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_25399, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000055 DASRWS010000000
VERSION     DASRWS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25399"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1330
                     /locus_tag="VFF72_00965"
     CDS             359..1330
                     /locus_tag="VFF72_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZV91751.1"
                     /translation="MQNVRIDSSSAGQRLDNFLAKILKGVPRTHLYRVIRSGEVRVNK
                     GRAGADTRLGLGDEVRIPPVRTADRSEVAPAPPREFPIVFEDEHLLAIDKPAGVAVHG
                     GSGESFGVIEQLRRARPDAEFLELVHRLDKETSGLLLIARTRKALVALQYDLRSRSAE
                     RAIGKTYAALVAGAWPDNRKVIDVALHKYVDADGARRVRTVAADDDQGRRSITLVRVA
                     ERWPAYSLLDVTIKTGRTHQIRVHLASEGHVVVGDPRYGDFALNKALARGTAVPGVRF
                     ERMFLHARRLRFVHPATGEAVSLEAPLPDACVRLLQAMRTAATEASS"
     gene            1327..1992
                     /locus_tag="VFF72_00970"
     CDS             1327..1992
                     /locus_tag="VFF72_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZV91752.1"
                     /translation="MSGAARRFDLIVFDWDGTLFDSTRLIARCIQSACADVGTAVPSD
                     RDAAFVIGLGLADALRHAAPALPRERYRELGDRYRHHYLSSQDELVLFEGTLDMLQAL
                     RARNHSLAVATGKTRRGLDDVLGRVELRGLFDATRTADETAGKPDPRMLHELMREIGC
                     APQRTLMVGDTTHDLQLAANAGCASVAVSFGAHEPEAFADFAPLHVAHSTAGLAAWLD
                     VHA"
     gene            1985..2425
                     /locus_tag="VFF72_00975"
     CDS             1985..2425
                     /locus_tag="VFF72_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZV91753.1"
                     /translation="MPERRLGALAPVAAVPAPRQKVCDSAALLERGRAHVFDVLRFGE
                     PARAFALRFDGVVVAYLNRCVHVPTELDWQPGEFLDASREFIICSIHGAAYAPHGGRC
                     IGGPCASGKLVALEVGEEAGVVWWQPTRDTRAAPAAPSQGSPSQ"
     gene            complement(2404..2895)
                     /locus_tag="VFF72_00980"
     CDS             complement(2404..2895)
                     /locus_tag="VFF72_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZV91754.1"
                     /translation="MTTPRSESLRQQLVRNFDSLELKREQRPPLYDTRSGRLSTGTAA
                     TKLGASFDILAPGMRSCPYHFHHAQEEMFVIVEGRGSLRVADELIDVRCGDVVFIPPG
                     PQYPHQFINTSDAPMRYLSISTREQPEICEYPDSAKYSAWAPGIRVQQRAGSNIDYWD
                     GEP"
     gene            complement(3099..3443)
                     /gene="mrtJ"
                     /locus_tag="VFF72_00985"
     CDS             complement(3099..3443)
                     /gene="mrtJ"
                     /locus_tag="VFF72_00985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JDVT-CTERM system glutamic-type intramembrane
                     protease"
                     /protein_id="HZV91755.1"
                     /translation="MSRSDRPLVDIAWLCVASVAEEVVFRGVLQPWLAKRFGRRLSAA
                     RLTPANLLTSAVFAALHAWRHPVAVAAGVFPVSLVYGIARERSGSVVPPALLHAGFNV
                     LLYAASWLVGPR"
BASE COUNT          542 a         1266 c         1276 g          564 t
ORIGIN      
        1 agctcgagga agcgggggat tgagccggcg gctgcgacct cgttctcgtc ttcaaccgga
       61 aaacctttga gtggattctt gtctgttcgc tctgccgtcc tgggtccggg gccgccgcgt
      121 cacttgggga acgcggcctg cacgaacgtc gagacgttgc atcaccatga tggccggaac
      181 atggatccgg ccgttcccgc tgcggcccga gctgcatcgc atacgcgacg ctaaactctg
      241 aaaaatcaaa tacttaggct gcggcgtgga aggacgaatt atagggggca aaggcggtcg
      301 gcgcggtacc gccgggtccg cccgtggcgc ccgcggcgaa gccgccgtgc cgccggtcgt
      361 gcaaaacgtc cgcatcgaca gcagctcggc cggccagcgg ctcgacaact tcctggccaa
      421 gatcctgaaa ggtgtgccgc gcacccatct ctatcgcgtc atccgctcgg gcgaagtgcg
      481 ggtcaacaag ggccgcgccg gggccgatac gcggctgggg ctcggcgacg aggttcgcat
      541 tccgccggtg cgaacggccg accgcagcga agtcgctccg gcgccgcccc gtgagttccc
      601 gatcgtcttc gaagacgagc acctgctcgc catcgacaag cccgccggcg tcgccgtcca
      661 cggcggcagc ggcgagagct tcggcgtcat cgagcagttg cgccgggccc ggccggatgc
      721 agagttcctc gagctcgtgc atcgcctcga caaggagacg tccgggctgc tcctgatcgc
      781 gcgcacgcgc aaggccctcg tcgcgctgca gtacgacctg cgctcgcgca gtgccgaacg
      841 cgcgatcggc aagacctatg cggcgctagt cgccggcgcc tggcccgaca accgcaaggt
      901 gatcgacgtc gcattgcaca agtatgtcga cgccgacggc gcccggcggg ttcgcacggt
      961 cgccgccgac gacgaccagg ggcgccgttc gatcacgctg gtgcgcgtcg ccgagcgatg
     1021 gcctgcctac agcctgctcg acgtgacgat caagaccgga cgcacacacc agatccgggt
     1081 ccacctggcc agcgagggcc acgtcgtcgt gggcgatccc aggtacggcg atttcgccct
     1141 caacaaggcg ctggctcgcg gcactgcggt ccccggcgtt cgcttcgaga gaatgttcct
     1201 gcacgccagg cgactgcgct tcgtccatcc ggcgacgggc gaagccgtca gcctggaagc
     1261 gccgttgccc gacgcctgcg tgcgcctgct ccaggcgatg cgcaccgcgg cgacggaggc
     1321 gtcgtcgtga gcggggcagc gcggcgcttc gacctgatcg tcttcgactg ggacggcacc
     1381 ttgttcgact cgacgcggtt gatcgcccgc tgcatccagt cggcctgtgc cgacgtcggc
     1441 acggcggtgc cgagcgatcg cgacgcggcc ttcgtgatcg gcctgggcct ggccgatgcg
     1501 ctgcgccacg cggcgccggc gctgccccgc gagcgctacc gcgagctcgg cgaccggtat
     1561 cggcaccact atctgtcgag ccaggacgag ctcgttctct tcgagggcac gctcgacatg
     1621 ctgcaggcac tgcgggcgcg caatcattcg ctggcggtcg ccacgggcaa gacgcggcgc
     1681 ggcctcgacg acgtgctcgg gcgggtcgag ctgcgcggcc tgttcgatgc gacccgtacc
     1741 gccgacgaga ccgccggcaa gcccgatccg cggatgctgc acgagctgat gcgcgagatc
     1801 ggctgtgcgc cgcagcgcac gctcatggtc ggcgacacga cgcacgacct tcagcttgcc
     1861 gccaacgccg gttgcgcgag cgtcgccgtc agcttcggcg cgcacgagcc cgaggctttc
     1921 gccgatttcg cgccgctgca cgtcgctcat tccacggccg gcctcgcggc ctggctggac
     1981 gtccatgcct gagcggcgtc tcggtgccct ggcgccggtg gccgcggtgc cggcgccccg
     2041 gcaaaaggta tgcgattcgg cagcgctgct cgaacgcggt cgcgcccacg tcttcgacgt
     2101 ccttcgattc ggcgagccgg cgcgcgcctt tgcactgcgc ttcgacggtg tcgtggtcgc
     2161 ctatctcaat cgctgcgtcc acgtcccgac cgagctcgac tggcagccgg gcgaattcct
     2221 cgacgccagc cgcgagttca tcatctgctc gatccacgga gcggcctatg cgccgcatgg
     2281 cggccgttgc atcggcggcc cgtgcgccag cggcaagctc gtcgcgctgg aggtgggcga
     2341 ggaggcgggc gtggtctggt ggcagcccac gcgcgacacc cgggcggcac ctgccgcgcc
     2401 gtctcagggc tcgccgtccc agtagtcgat gttggaaccg gcgcgttgct ggacacggat
     2461 tcccggcgcc caggcgctgt acttggcgga gtcggggtac tcgcagatct cgggttgctc
     2521 cctggtgctg atgctcaggt agcgcatcgg cgcgtccgag gtgttgatga attgatgcgg
     2581 gtattgcggc cccgggggga tgaagacgac atcgccgcag cgtacgtcga tcagttcatc
     2641 ggcgacgcgc aggctgcctc ggccttcgac gatcacgaac atctcctcct gcgcgtggtg
     2701 gaagtggtag gggcagctgc gcatccccgg cgccaggatg tcgaagctgg cgcccagctt
     2761 cgtggccgcg gtgcctgtgg aaaggcggcc ggagcgcgtg tcgtagagcg gcggccgttg
     2821 ctcgcgcttc agctcgaggc tgtcgaagtt gcgcaccagt tgctggcgga gcgactcgga
     2881 tcgcggcgtc gtcatggcga attcctcctc gtgctggagg ggtcatcgta gcggcgcacg
     2941 aggcgacggg cgcacggtcg acgccgctgg ggacgtcacg ccgctgtcac ctccgcgcgc
     3001 gtgccatgcg caccgaagct gccaggacgc gagggccggt gaccgaacgc attcctcggt
     3061 gatgcgcgcg ccggcgcccc ggcgcttcgg ctcgaggctc accgcggccc gaccagccac
     3121 gacgccgcgt acagcaggac gttgaagccg gcgtgcagca gcgccggcgg cacgacgctg
     3181 ccgctgcgct cgcgtgcgat gccgtagacg agggagacag ggaagacgcc ggcggcgacc
     3241 gcgaccggat ggcgccacgc gtgcagcgcc gcgaagacgg cgctcgtcag gagattggcc
     3301 ggggtcagcc gtgcggccga gagtcgccgt ccgaagcgct tcgcgagcca cggttgcagg
     3361 acgccacgaa acaccacttc ctcggcgacc gacgcaacgc acaaccaggc gatgtcgacc
     3421 agcggtctgt cgcttcgcga catcgtgaag cctgcagccg cgaccgcgac ggcgatcggt
     3481 ggtgcgagca aggcacacgc ccaccagaaa gcgcgtgacg gaaggcgtct catcgttcga
     3541 ttcgcgcagg cagtgtgcac tcttccggac ggcccctgga ccgagccttc gtcaacgacc
     3601 gatctcgtcg ggcgcgacgc gcggcgaccc ttctcccctc agctccgc
//
LOCUS       DASRWS010000056         4060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1176326, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000056 DASRWS010000000
VERSION     DASRWS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176326"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..508)
                     /locus_tag="VFF72_00990"
     CDS             complement(263..508)
                     /locus_tag="VFF72_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91756.1"
                     /translation="MNAPARLPLLRRGALAVGLMAAVAMLIGFYSIVVGAVEHGAHRR
                     LMASVAPAHVAPRVATTRLQGGVKDATHRISLASASD"
     gene            1030..1866
                     /locus_tag="VFF72_00995"
     CDS             1030..1866
                     /locus_tag="VFF72_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZV91757.1"
                     /translation="MLIRIGYDIELSVTTPMALIYLLRLHPSRRGDLVAPENFHISDG
                     LVAEEYTDSFGNHCGRVNVPAGLSTVRLTNDALIRDSGELDELNDDARQHDPTELPVA
                     TLQFLLPSRYCEVDSELLGFAWDRFGKTPQGTPRVRAICDFVHNHLRFDYQAARPTRT
                     ALDGYRERQGVCRDFTHLAITLCRCMNIPARYTTGYLGDIGVPIAPGPMDFSAWFEVF
                     LGDRWYTFDARHNRRRIGRVVIARGRDAGDVPITMCFGTNSLLRFDVVTEEVVEREPL
                     AA"
     gene            1920..2153
                     /locus_tag="VFF72_01000"
     CDS             1920..2153
                     /locus_tag="VFF72_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91758.1"
                     /translation="MNNPHDKDRVQGEGDYDAARRYDKQSESFAKSGKVDKAAHDATP
                     DSPQQAEEMRKAEEVGKSHSKGEGPLDSKKRKA"
     gene            complement(2235..>4060)
                     /locus_tag="VFF72_01005"
     CDS             complement(2235..>4060)
                     /locus_tag="VFF72_01005"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HZV91759.1"
                     /translation="AGKPATFPTFGQLRVGDGHVVYDDAILPAHIDARLALNDGSDLA
                     EASALSNATPSGGASAASGAMASASEAARSASSPDGSKAGIVVRAGRPAAPASALAER
                     VTLDPGQVGLSLKAVGSYRNLPLRIDLRTDGVLRLFLAGSEAKAQPLRLLASIGRADL
                     SFEGSTTDPLHFAALRGRFTLAGPSLAAVGDPLGVTLPTTPAFKTHGTLAKDGAVWNA
                     VFESASVGSSRLTGAFTYDTRPKVPLLSGRLAGARLVLADLGPAVGTPPSGAPVSTRD
                     DRVIPDKKFDLPSLRAMDANVIVDIDMFDPGTALIEPLRPVRAHILLADGVLTIADFE
                     GDTADGRLVGYLQLDGRKSEALWTADMRAVGLDLSKWLRLKRKGDAPPYVSGRLDAMV
                     KVKGQGRSTAEILSTLNGDIRAHLRKAEISHLAIEVAGLDVAQALGVFVKGDDALPVL
                     CNVADLDVANGLVTPRVFVIDTKDSTIWIDGTISLKTEGLDLKAVVSPKDFSPLTLRT
                     PIRVTGVFGKPKVSLDLGKLAGKTGAAALLALLNPLAAIIPFIDPGSRGTARHEDEEC
                     ANLVKTRGVISRATRTPANVRVPAAAIRGATPASSASSAGR"
BASE COUNT          676 a         1406 c         1382 g          596 t
ORIGIN      
        1 gaggatggca gacaggccga gccggtggct acggccgagc acggcttcgc agcgcacgca
       61 tcgggcgacc acgtggccgg cgagcgggcg ccagcggtgc gccatgccgc attgctcgca
      121 caccacgagt gaatgctcgt cacggctcat cgcccggggt tctccgccgc gccatgcggc
      181 caggcgccat tatggtgacc ccgggtccga caacttccag gggaggcccc gtcgatcccg
      241 gtcacggcgt cgggagacgg gatcagtcgc tggcgctggc cagcgagatg cggtgcgtcg
      301 cgtccttgac gccgccctgc aagcgcgtcg tcgccacccg tggggcgaca tgggccggcg
      361 cgaccgatgc catgaggcgt cgatgcgcac cgtgttccac cgcgccgacg acgatcgaat
      421 aaaagccgat cagcatcgcc accgcggcca tcaatccgac ggcgagtgcg ccacgccgca
      481 gcaacggcaa cctggcaggg gcgttcatgc ggacaccctc gggaccgagc ggtgcgatga
      541 agcccgtagg accctggcgg cgacttctcg cctcggccga tcgccaacga tggacagcgt
      601 cgacctgtgc ttgatgtcgc gtccgcgcga gtgcttcgat tcgcggcccg gttctcgacc
      661 cttgggaccg tcacttggcg gccgcttggc aggcccgcca cgcggcgcga tatgcgtgac
      721 ctccggcgac gaattgacga accagcggac gaaatggtcg atgcgttcgg acagcgtggt
      781 catgcgagtc tcctgtgagc agactacgca cgccggttcg aaagcaaagc cagtggagcg
      841 cgccctcgct cgtcggacgc cacctacttc cgatgaccgg cccgctgtcc gtgaaggggt
      901 cgcggcttgc atcgcagccg cacccacgcg ccgctttcct acagatggca cagccgaaag
      961 cggttgcgcg acgaggcgtc gacgtgagac tctctcgtgc gccggcctcg ggaggccccg
     1021 gagttcagca tgctcatacg aatcggctat gacatcgagt tgtccgtcac gacgccgatg
     1081 gccctcatct acctgctgcg attgcatcca tcgcggcgcg gcgatctggt tgcgccggaa
     1141 aacttccaca tcagcgacgg gctcgtcgcc gaggaataca ccgacagctt cggcaaccac
     1201 tgcgggaggg tcaatgtccc ggccggcctg tcgaccgtgc gtctcaccaa cgacgcgctg
     1261 atccgggact cgggcgagct cgacgagttg aacgacgacg cccgccagca cgatccgacg
     1321 gagctgccgg tggcgacctt gcaatttctc ctgcccagcc gctactgcga ggtcgacagc
     1381 gagctgctcg gcttcgcctg ggaccgcttc ggcaagacgc cgcagggaac gccccgggtt
     1441 cgcgccatct gcgatttcgt ccacaaccac cttcgcttcg actaccaggc cgcgcggccg
     1501 acccgcacgg cgctcgacgg ctaccgcgag cgccagggcg tgtgccggga tttcacgcat
     1561 ctggcgatca cactgtgccg gtgcatgaac atcccggcgc gctacaccac cggctacctg
     1621 ggcgacatcg gcgttccgat cgcaccgggg ccgatggact tcagcgcctg gttcgaggtc
     1681 ttcctgggcg accgctggta caccttcgat gcccggcaca accggcgccg catcggccgc
     1741 gtcgtcatcg cccgcgggcg cgatgccggc gacgttccca tcaccatgtg cttcggaacc
     1801 aactcgctgc tccggttcga cgtcgtcacc gaagaggtgg tcgagcgcga gccgctggct
     1861 gcatgacccg ggccgcgtca agccgacgcg gcatttccac tcactgcaaa ggagagacga
     1921 tgaacaaccc ccatgacaag gaccgcgtcc agggcgaagg cgactacgac gcggctcgcc
     1981 gctacgacaa gcaaagtgaa tccttcgcga agtcgggcaa ggtcgacaag gcagcgcacg
     2041 acgccacccc cgactcgccg caacaggccg aggaaatgcg caaggccgag gaagtcggca
     2101 agtcgcattc gaagggcgag gggccgctgg acagcaagaa gcgcaaggcc tgaagcgtcg
     2161 gcggcgacac gccgccgacg tcgggtcgat gaaggcacga cggcacgtcg tgcctggttc
     2221 cggtcagcgg ccgatcagcg gccagccgaa gaagccgaag aagccggcgt cgcaccgcga
     2281 atcgcagctg ccggaacgcg cacgttggcc ggcgtgcggg tcgcccgcga gatgacgcct
     2341 cgggtcttga ccaggttggc gcattcctcg tcttcgtgcc tggcggtgcc gcgcgacccc
     2401 ggatcgatga acgggatgat cgccgcgagc ggattgagca gcgccagcag cgctgcggcg
     2461 ccggtctttc ctgccagctt gccgaggtcc agcgacacct tcggcttgcc gaagacaccc
     2521 gtcacgcgga tgggcgtgcg caaggtgagc gggctgaagt ccttcggcga gacgaccgcc
     2581 ttcaggtcca ggccctcggt cttcagagaa atggtgccgt cgatccagat ggtcgaatcc
     2641 ttggtgtcga tcacgaacac cctcggcgtg accagaccgt tggcgacgtc gaggtcggcg
     2701 acgttgcaca ggacgggcag tgcgtcgtcg cccttgacga agacgcccag ggcctgggcg
     2761 acgtcgagcc cggcgacctc gatggccagg tgcgagatct ccgccttgcg caggtgcgcc
     2821 cggatgtcgc cgttgagggt cgagaggatc tcggccgtcg agcggccctg ccccttcacc
     2881 ttgaccatgg catcgagccg cccggagacg tacggaggcg cgtcgccctt gcgcttgagc
     2941 cgcagccact tcgacaggtc gaggccgacg gcccgcatgt cggcggtcca caaggcctcc
     3001 gatttccgcc cgtcgagctg caggtagccg acgagccggc cgtcggcggt gtcgccttcg
     3061 aagtcggcga tggtcagcac gccgtcggca agaaggatgt gcgcgcgcac cggacgcagc
     3121 ggttcgatca gggccgttcc gggatcgaac atgtcgatgt cgacgatcac gttggcgtcc
     3181 attgctcgaa gcgacggcag atcgaacttc ttgtccggga tcacgcgatc gtcgcgcgtc
     3241 gacaccggcg cgccgctggg cggtgtgccg acggcaggcc ccaggtcggc gagcacgagg
     3301 cgagcgccgg cgaggcgccc cgacagcagc ggcaccttcg gtcgcgtgtc gtaggtgaag
     3361 gcgccggtaa gccggctgct gccgaccgac gccgattcga agacggcgtt ccacaccgcg
     3421 ccatccttcg cgagcgtgcc gtgcgtcttg aatgccggcg tcgtcggcaa ggtgacgccg
     3481 agcggatcgc cgactgccgc cagcgacggc ccggccagcg tgaagcgacc cctcagggcg
     3541 gcgaagtgga gcggatcggt cgtgctgcct tcgaacgaca ggtcggcccg gccgatcgac
     3601 gccagcagcc gaagcggctg cgccttggcc tccgagccgg ccagaaagag cctcagcacg
     3661 ccgtcggtgc gcagatcgat ccgcaacggc aggttccggt agctgccgac cgccttgagg
     3721 ctcaatccga cctgtcccgg atccagggtg acgcgctcgg ccagcgccga agccggtgcc
     3781 gccggacggc cggcccgcac gacgatgccc gccttgctgc cgtcgggcga gctcgccgac
     3841 ctcgccgcct cgctcgccga agccatggcg ccgctcgccg ccgaagcgcc gcccgacggt
     3901 gtcgcgttcg aaagcgccga ggcctcggcg agatcgctgc cgtcgttcaa cgcaagacgc
     3961 gcgtcgatgt gcgcgggcag gatcgcgtcg tcgtacacga cgtggccgtc gccgacccga
     4021 agctggccga acgtcggaaa cgtcgccggc ttgccggcgg
//
LOCUS       DASRWS010000057         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_316651, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000057 DASRWS010000000
VERSION     DASRWS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316651"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFF72_01010"
     CDS             <1..291
                     /locus_tag="VFF72_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91760.1"
                     /translation="NLHVRGYKEEGTITTPFDMRVQNDLDRFHLVQDVVDRLPDLCSK
                     GAYLKQLTQDKLIEHKHYIDRHGQDMPEIRNWKWGASAGANAADERATGDRT"
     gene            288..1235
                     /locus_tag="VFF72_01015"
     CDS             288..1235
                     /locus_tag="VFF72_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZV91761.1"
                     /translation="MNPITSFLVFICILAGVATGYFVNPYLAIIFFAVGALIAASLKM
                     ANVWQKFVILRMGKLQSVKGAGLFAIIPVIDSIVAVIDCRIQTTAFNAEQALTRDTVP
                     VNVDAIIFWHVEDAEKAALAITHYREAIDRVAQTSLREMIGSSMLTALLSDRKDADEQ
                     LKVEIGAKAAPWGISVGSVEIRDVAIPEALQNAMSRQAQAEREKQARVILGSAEAAIA
                     GKFVEAANIYDGHPWALQLRAMNIIYETTKERGATILMPSSMVDSLNPSAATLAFALA
                     GKVVPTPELDEAPEPAMRQSTPRPAPAAEETDPQSLMPA"
     gene            1240..>4129
                     /locus_tag="VFF72_01020"
     CDS             1240..>4129
                     /locus_tag="VFF72_01020"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HZV91762.1"
                     /translation="MKAAADSPPKDPEPKPDARPGAKPAPKDELKTLPIAEVEKKLGS
                     SPGGLSDAEAKKRLTQYGPNEIVEKKTNALLKLLSYFWGPIPWMIEVAVVLSGVVGHW
                     PDFFIILVLLVANAAVGFWEERQAGNAIEALKARLAIKARVRRDGKWINPPARELVPG
                     DAIRLRLGDIVPADARLLGGDEVSVDQSALTGESLPKTCKTGDAVFSGSIIRRGEATA
                     LVYATGGKTYFGKTAELVQTAVTVSHFQKAVLKIGNYLIMLAVVMVAVIIGMGIYRGE
                     HILTVLQFALVLTVAAIPVAMPTVLSVTMAVGARLLAKKQAIVSKLVAIEELAGVDVL
                     CADKTGTLTQNRLSLGEPFCLAKTTAGQLILAGALASRAENNDTIDLAVLAGLKDKAA
                     LKPFRVTHFQPFDPVHKRTEATVKAADGSTFKVSKGAPQVILALAANAAAVKKAVDKA
                     VDDFAARGFRALGVARADGDGPWQLLGVLPLFDPPREDAKATVATAAQMGVKIKMVTG
                     DALAIAKETAKTLGMGTNILDGASLGDSKTEETEAVTRSIEDADGFAQVYPEHKFHIV
                     DILQKNGHIVGMTGDGVNDAPALKKADCGFAVSGATDAARAAAAIVLVAPGLSVVIDA
                     LKESRKIFQRMNSYAMYRIAETLRVLLFMTLAIVVFNFYPLTAIMIVMLALLNDGAIL
                     SIAYDRVRYSDRPEAWNMRMVLGIATVLGLVGPIAAFGLFFLGDRVFHLGHPQLQTMM
                     YLMLSVAGHLTIFQTRTRGPWWSTRPAWILLAAVCGTQVVATLIAVYGAWLVTPLGWR
                     YTGLVWGYAFAWFLLTDPVKLLAYKVLDKVKADVRPQHEAKRDPGRDDAKAAPDANAA
                     PDANAAPDAKAAPDAKAAPDANAAPDANAYPDDKAAPDAKAEREAKVAADPKPAPDAK
                     AYTPSDSHDTSDAKPDAGPKVPSPSDLTPQLVKRVHDLYEQLGREEVRA"
BASE COUNT          745 a         1374 c         1364 g          646 t
ORIGIN      
        1 aacctgcacg tgcgcggcta caaggaagaa ggaacgatca ccacgccgtt cgacatgcgc
       61 gtgcagaacg acctcgaccg cttccatctg gtgcaggacg tcgtcgatcg gctgcccgac
      121 ctgtgcagca agggtgccta cctgaagcag ctgacgcagg acaagctcat cgagcacaag
      181 cactacatcg accggcatgg gcaggacatg ccggagatcc ggaactggaa gtggggagcg
      241 tccgcgggtg cgaacgcggc cgacgaacgc gccaccggag acagaacatg aatccgatca
      301 cctcgttcct ggtcttcatc tgcattcttg cgggcgtcgc caccggctat ttcgtgaatc
      361 cgtatctcgc catcatcttc ttcgccgtcg gcgccctcat cgccgcctcg ctgaagatgg
      421 ccaacgtctg gcagaagttc gtcatcctgc gcatgggcaa gctccagagc gtcaagggcg
      481 ccgggctgtt cgccatcatc ccggtcatcg acagcatcgt cgcggtaatc gactgccgca
      541 tccagacgac agccttcaac gccgagcagg cgctgactcg cgacacggtt cccgtcaacg
      601 tcgacgcgat catcttctgg cacgtggaag atgccgagaa ggcggccttg gcgatcaccc
      661 actaccgcga ggccatcgac cgcgtcgcgc agacctcgct gcgcgagatg atcggctcgt
      721 cgatgctgac ggcgctgctg tcggatcgca aggacgccga cgagcagctg aaggtcgaga
      781 tcggcgccaa ggccgcgccc tggggcatct cggtcggctc ggtggagatc cgcgacgtcg
      841 ccattcccga agcgctccag aacgcgatgt cgcgccaggc ccaggcagag cgcgagaagc
      901 aggcccgcgt catcctcgga tccgccgagg cggcgatcgc cggcaagttc gtcgaggcgg
      961 ccaacatcta cgacggccac ccgtgggcgc tgcagcttcg cgccatgaac atcatctacg
     1021 agacgacgaa ggagcgaggc gccacgatcc tgatgccgag ctcgatggtc gacagcctca
     1081 atccttccgc ggccacgctg gcgttcgccc tggcgggcaa ggtcgtgccg acgcccgagc
     1141 tcgacgaggc gccggaaccg gcgatgcgtc aatccactcc gcgcccagcg ccagccgcgg
     1201 aagagacgga tccgcagtcc ttgatgccgg catgacttca tgaaggccgc cgctgactcg
     1261 cctcccaagg atcccgagcc gaaacccgac gccaggccgg gagcgaagcc ggcgcccaag
     1321 gacgaattga agacgctgcc gatcgccgag gtcgagaaga agctcggctc ctcacccggc
     1381 gggctgagcg acgccgaggc gaagaagcgg ctgacccagt acgggccgaa cgagatcgtc
     1441 gagaagaaga ccaacgcgct gctgaagctg ctctcctatt tctggggacc catcccctgg
     1501 atgatcgagg tcgccgttgt gctgtccggc gtggtcggcc actggcccga cttcttcatc
     1561 atcctggtat tgctggtcgc caatgccgcg gttggattct gggaggagcg ccaggcgggc
     1621 aatgcgatcg aggccctgaa ggccaggctg gcgatcaagg cgcgggtcag gcgcgacggc
     1681 aaatggatca acccgccagc gcgcgagctc gtgccgggcg acgccattcg cctgcgactg
     1741 ggcgacatcg tgcctgccga tgcgcgtctg ctgggcggcg acgaggtctc cgtcgatcag
     1801 tcggcgctca ccggggaatc cctgcccaag acctgcaaaa cgggcgacgc cgtgttctcc
     1861 gggtcgatca tccgtcgcgg cgaggccacc gcgctggtct atgcgacggg cggcaagacc
     1921 tacttcggca agaccgcgga actggtgcag acggccgtca cggtcagcca cttccagaag
     1981 gccgtgctga agatcggcaa ctacctgatc atgctggcgg tggtcatggt cgcggtgatc
     2041 atcggcatgg gcatctatcg cggcgagcac atcctgaccg tgctgcagtt cgccctggtg
     2101 ctgacggtgg cggccattcc ggtggcgatg ccgacggtgt tgtcggtgac gatggccgtc
     2161 ggcgcgcgcc tgctcgcgaa aaagcaggcg atcgtcagca agctggtggc gatcgaggaa
     2221 ctggccggtg tcgacgtgct gtgcgccgac aagacgggca cgctgacgca gaacaggctg
     2281 agcctgggcg agccgttctg cctcgccaag accaccgccg ggcagctcat cctggccggc
     2341 gcgctggcct cgcgcgcgga gaacaacgac accatcgacc tggccgtgct cgctggtctg
     2401 aaggacaagg cggcgctgaa acccttccgg gtgacgcact tccagccgtt cgatccggtg
     2461 cacaagcgca ccgaggccac cgtcaaggcc gccgacggca gcaccttcaa ggtgagcaag
     2521 ggcgcgcctc aggtcatcct cgcgctggcg gccaatgcgg cggcggtgaa gaaggccgtc
     2581 gacaaggccg tcgacgactt cgccgcgcgc ggctttcggg cgctcggcgt cgcccgtgcg
     2641 gatggcgatg gcccgtggca gctgctcggc gtgctgccgc tgttcgatcc gccgcgcgag
     2701 gatgccaagg ccaccgtcgc gaccgcggcg cagatgggcg tcaagatcaa gatggtgacc
     2761 ggcgacgcgc tggccatcgc caaggagacc gccaagacgc tcgggatggg cacgaacatt
     2821 ctcgatggcg ccagcctcgg cgattcgaag accgaggaga ccgaagcggt cacccggtcg
     2881 atcgaggatg ccgatgggtt cgcccaggtc tatccggagc acaagttcca catcgtcgac
     2941 atcctgcaga agaacggcca catcgtcggc atgaccggcg acggagtgaa cgacgccccg
     3001 gcgctgaaga aggccgattg cggcttcgcc gtttccggcg cgaccgacgc ggcgcgcgcc
     3061 gccgcggcga tcgtgctggt cgcgccgggg ctctcggtgg tcatcgatgc gctcaaggaa
     3121 agccgcaaga tcttccagcg catgaacagc tacgcgatgt accgcatcgc cgagaccttg
     3181 cgcgtcctcc tgttcatgac gctggcgatc gtcgtcttca atttctatcc gttgacggcg
     3241 atcatgatcg tcatgctggc gctgctcaac gacggcgcca ttctgtccat cgcctacgac
     3301 agggttcgct acagcgaccg gcctgaagcc tggaacatgc gcatggtcct gggcatcgcg
     3361 acggtgctgg gcctggtcgg cccgatcgcc gcgttcggtc tgttcttcct gggcgaccgc
     3421 gtgtttcacc tgggccatcc gcagcttcag acgatgatgt acctgatgtt gtcggtggcc
     3481 ggccatctga cgatcttcca aacccgaacg cgcggcccgt ggtggtccac ccgcccggcg
     3541 tggatcctgc tcgcagccgt gtgcgggacg caggtcgtgg caaccctgat cgcggtctac
     3601 ggcgcctggc tggtgacgcc gctgggctgg aggtacacag gcctggtctg gggctacgcc
     3661 ttcgcctggt tcctgctgac cgacccggtg aagctgctcg cctacaaggt gctcgacaag
     3721 gtcaaggccg acgtcaggcc gcagcacgaa gcgaagcgag atcccgggcg cgacgacgcc
     3781 aaggcagcgc ctgatgccaa tgctgcgccc gatgccaacg cagcgcctga tgcgaaggct
     3841 gcgcccgatg ccaaagcagc gcctgatgcg aacgctgcgc ctgatgccaa cgcctatccc
     3901 gatgacaagg ctgcgcctga cgccaaggcc gagcgcgagg ccaaggttgc cgccgacccg
     3961 aagcctgcgc ccgacgcgaa ggcgtacacc ccgtctgatt cccatgacac gtccgacgca
     4021 aagcctgatg ccgggcccaa ggtgccgtca ccgtcggact tgaccccgca gctcgtcaaa
     4081 cgggtgcacg acctctacga gcagctgggg cgggaagagg tgcgcgccg
//
LOCUS       DASRWS010000058         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4055772, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000058 DASRWS010000000
VERSION     DASRWS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055772"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFF72_01025"
     CDS             complement(<1..243)
                     /locus_tag="VFF72_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZV91763.1"
                     /translation="MNDIRRTVLWGIFSISLFLIWNAWSIHNGQPSFFAPRPTPAAPA
                     AAAAHPGAGIAAAGPASAVAATALPSVPATPSAGTTA"
     gene            complement(240..542)
                     /gene="yidD"
                     /locus_tag="VFF72_01030"
     CDS             complement(240..542)
                     /gene="yidD"
                     /locus_tag="VFF72_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZV91764.1"
                     /translation="MATRCLVALVRAYRLLLSPWLGNSCRFEPTCSNFSLQALTKHGA
                     VAGTVLTVGRIARCHPWCAGGHDPVPQQLPRWLQPLSTSNGVDDIPTPAETDRATP"
     gene            complement(548..1000)
                     /locus_tag="VFF72_01035"
     CDS             complement(548..1000)
                     /locus_tag="VFF72_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZV91765.1"
                     /translation="MQRLVESAEFERVLATASRARTAHFAIHHGAAALPPALFGPFTT
                     KLSTIEAETGAPLVDDLSLTAPFHRLGAVVPKRHARRAVTRSLLKRQIYAAGARHAPA
                     LVGGTWVVRLRAPFDRRDYPSAASAALRESARGELDALFAAASRGSKP"
     gene            complement(1048..1182)
                     /gene="rpmH"
                     /locus_tag="VFF72_01040"
     CDS             complement(1048..1182)
                     /gene="rpmH"
                     /locus_tag="VFF72_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZV91766.1"
                     /translation="MKRTYQASKVRRARTHGFLVRMKSRGGRAVINARRAKGRKRLAV
                     "
     gene            1383..>2649
                     /gene="dnaA"
                     /locus_tag="VFF72_01045"
     CDS             1383..>2649
                     /gene="dnaA"
                     /locus_tag="VFF72_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZV91767.1"
                     /translation="MRADLWQRGCERLAADLPDQQFNTWIRPLADAEVDDLGDGGATV
                     TIRVPNRFKLDWIRAQYAGRIEQTLSDIAGKLVRLDILLAARDSHSRPLPLAEGRASA
                     AVASASAPAVVRGPATPLPEAMLPSRSRLNPSLSFDTLVPGRANQMARTAALHVAGAP
                     GQMYNPLFIYGGVGLGKTHLIHAVGNALLTDRPDARILYLHAEQFITDVVKNYQRKTF
                     DELKAKYHSLDLLLIDDVQFFAGKERTQEEFFNAFEALLAKRAHIIMTSDTYPKGLVD
                     IDERLTSRFDAGLTVAIEPPELEMRVAILIKKAEAEATTMPEDVAFFVAKNVRANVRE
                     LEGALRKVLAYSRFSHKEINIGLAREALKDLLSIQNRQVGVENIQKTVADFYKIKVAD
                     MYSKKRPASIARPRQIAMYLAKEMTQKSLP"
BASE COUNT          482 a          879 c          899 g          389 t
ORIGIN      
        1 cgccgtcgtt ccggccgacg gcgtcgccgg gacggacggc agcgctgtcg ccgcgacggc
       61 actcgcgggc ccggccgccg cgatgccggc gccaggatgc gcggctgccg ccgcgggcgc
      121 ggcgggtgtc ggtcgcggtg cgaagaacga cggctggccg ttgtgaatgc tccaggcatt
      181 ccagatcagg aacagcgaga tcgagaagat gccccagagg acggttcggc ggatgtcgtt
      241 catggcgtgg cgcgatcggt ttcggccggg gtcgggatgt cgtccacgcc gttggaagtg
      301 gaaaggggct gcagccagcg gggcagctgt tgcggcacgg gatcgtggcc gccggcgcac
      361 caggggtggc aacgggcgat ccggccgacc gtcagcacgg tgccggcgac ggcgccatgc
      421 ttcgtcaagg cctgcagcga aaagttcgag caggtcggct cgaagcggca cgaattcccc
      481 agccacgggc tcaggagaag tcgatacgcg cgaacgagag cgacaagaca gcgggtggca
      541 atggcgttca cggcttcgag cctctcgagg cagcggcgaa caacgcatcg agctcgcccc
      601 gggcactctc ccgcaaggcg gcggaagcgg cgctcggata gtcgcgacgg tcgaagggag
      661 ctcgcagacg cacgacccag gtgccgccga cgagcgcagg ggcgtgccgg gcgccggcgg
      721 cgtagatctg tcgtttgaga agcgaacggg tgacggcgcg gcgggcgtgt cgcttcggta
      781 cgaccgcacc cagtcggtgg aacggcgccg tcagcgacaa gtcatccaca aggggtgcac
      841 cggtttctgc ctcgattgtt gataacttcg tcgtgaacgg tccgaaaagg gccggcggca
      901 gtgccgccgc gccatggtgg atcgcgaaat gagccgtgcg ggcacgactc gccgtggcaa
      961 gaacgcgctc gaattcggcg gactcgacga gacgttgcac aaccgagacg atcggcgagc
     1021 cgccggcgcg ggccgccgtg gcgggcctca gacagccaga cgcttgcgtc ccttggcccg
     1081 acgcgcgttg atgaccgccc ggccgccgcg cgacttcatt cgaacgagaa agccgtgggt
     1141 tcgggcacga cggaccttgg aggcttggta ggtacgtttc atgatggatc cagtggcccg
     1201 agcgagtgca tcgaggcaaa ccggcaatta catcaaagct gggggaagac gtcaatcttc
     1261 ccgcgtcgtc gaacgggaga ttcgccatcg aacgcggccc gcgggctttc cctgtggata
     1321 agtgattcca gccgaccatc tcccgggtac aatcgaccgc ttttcgaggt acttctccac
     1381 acatgcgagc cgacctctgg caacgcggct gcgagcgtct cgccgccgac ctgcccgatc
     1441 agcaattcaa cacctggatc cgtccccttg ccgacgccga ggtcgatgac ctcggtgacg
     1501 gcggcgcgac ggtgacgatt cgcgtcccga accggttcaa gctcgactgg atccgggcgc
     1561 agtacgccgg tcgcatcgag caaacgctca gcgacatcgc cggcaagctg gtgcgtctcg
     1621 acatcctgct cgcagcgcgc gacagtcatt ctcggcccct gccgcttgcc gaggggcggg
     1681 cgagcgccgc cgtcgcttcg gccagcgcgc cggcggtcgt ccggggcccc gcgacgccgc
     1741 ttccggaggc catgctgcct tcacgaagcc ggctcaaccc gagcctcagc ttcgacacgc
     1801 tggtgccggg ccgcgccaac cagatggcgc gcaccgcggc cctgcacgtg gccggagcgc
     1861 cagggcagat gtacaacccc ctcttcatct acggcggcgt cggcctgggc aagacgcacc
     1921 tgatccatgc cgtcggcaac gcccttctca cggatcgccc cgacgcccgc atcctctacc
     1981 tgcacgccga gcagttcatc accgacgtcg tcaagaacta ccagaggaag accttcgacg
     2041 agctgaaggc gaagtaccac tcgctcgacc tgctgctgat cgacgacgtc cagttcttcg
     2101 ccggcaagga gcgcacgcag gaggagttct tcaacgcctt cgaggcactg cttgccaagc
     2161 gcgcgcacat catcatgacc agcgacacct acccgaaggg cctggtcgat atcgacgagc
     2221 ggctgacctc ccgcttcgac gccggcctca ccgtcgcgat cgagccgccc gagctcgaga
     2281 tgcgcgtcgc gatcctgatc aagaaggccg aggccgaggc gacgacgatg cccgaggacg
     2341 tcgccttctt cgtcgccaag aacgtgcgcg ccaacgtgcg cgagctcgaa ggcgcgctgc
     2401 gcaaggtgct cgcctactcg cgcttctcgc acaaggaaat caacatcggc ctggcgcgcg
     2461 aggcgctgaa ggacctgctg tcgatccaga acaggcaggt cggcgtcgag aacatccaga
     2521 agacggtggc cgacttctac aagatcaagg tcgccgacat gtactcgaag aagcggccgg
     2581 cgtcgatcgc gcggccgcgc cagatcgcga tgtatctggc gaaagagatg acgcagaaga
     2641 gtctgcccg
//
LOCUS       DASRWS010000059         4150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1464590, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000059 DASRWS010000000
VERSION     DASRWS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464590"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1194)
                     /gene="alr"
                     /locus_tag="VFF72_01050"
     CDS             complement(73..1194)
                     /gene="alr"
                     /locus_tag="VFF72_01050"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZV91768.1"
                     /translation="MPRPIEALVHLDALEANLAVARRAAGGARVWAVVKADAYGHDID
                     QAYAGLKGADGFALLDLAEAERLRDLGWRGAILLLEGCFGPRDLDACSRLNLWHTVHR
                     TAQIDWLAAHKTEHAHRIFLKLNSGMNRLGFGVDAYRAAWLRLDALPQVDAITLMTHL
                     ANADASDTASLEAALAVFAEATAELPGERSVCNSAATLRLGAARAGDWVRPGIMLYGS
                     SPDHPLHTAAAWGLQPAMTLRSELIAVQDVAAGAGIGYGSRYVAERPMRIGVVACGYA
                     DGYPRVAPGGNDRGTPVLVDGVRTRIVGRVSMDMITVDLTPAPRAGVGSEVVLWGRAA
                     SGDVLPIDEVAAAAGTIGYELMCARAPRVPVRAVGAPTP"
     gene            1322..2599
                     /gene="lplT"
                     /locus_tag="VFF72_01055"
     CDS             1322..2599
                     /gene="lplT"
                     /locus_tag="VFF72_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HZV91769.1"
                     /translation="MKKGFYSIMAAQFFSSLADNALLVAAIELLRTGGAPSWHIPALT
                     PIAALFYVLLAPVVGAFADAVPKGIVMFVSNAIKVIGCLLILFGADPLLSYGVVGLGA
                     AAYSPAKYGILTELLPNSQLVKANGWIEGLTILSIILGVLLGGQLLGHVVSQQLLSFD
                     MPLIDTGISTAPEAAVAVIVAIYVVAALFNLRIPRTEAPLQPLSSSIGALIRDFSNCN
                     ARLWADKLGQISLATTTLFWGVSGNLRVIVFAWAAIALGYSTKDASSLVGVVAIGTAA
                     GAILASMRMRLDQATRVIPLGIAMGVLVIGLDVIRSPWIAAPFLVVLGAIGGYLVVPM
                     NALLQHRGANLMGAGRSIAVQNFNEQACILGLGAFYTGMTRFGLSAFTAIGVFGVLVT
                     AMMWMIRRWHATNCVRYPEEIAHLLYIARLDKH"
     gene            2596..3516
                     /locus_tag="VFF72_01060"
     CDS             2596..3516
                     /locus_tag="VFF72_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZV91770.1"
                     /translation="MNDASPTSSRQTLLASAALVFNALVWGTSWWPFRKLHDAGLHPL
                     WATAIVFGVASLVIVAARPRALAQVLRTPALWLLVAAAGTTNAAFNWAVVIGDVVRVV
                     LLFYLMPLWTVLLARVVLGERLSAAAALRIALALVGAAIVLWPPSTGTAPSSWAERLP
                     LPRSLADWLGIVGGFSFALNNVMLRREAAQPEEGRALAMFIGGAVVAGILASVLTASG
                     ALAAPPPSAAWLWPAAALTTLFLGSNLALQYGAARLPANRTSVVMLTEVVFASASSVA
                     FGGAEPTARTVLGGGLIVASAVFAALAPSR"
     gene            3577..4074
                     /locus_tag="VFF72_01065"
     CDS             3577..4074
                     /locus_tag="VFF72_01065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZV91771.1"
                     /translation="MADTPHSIYDFEAPSIQGQTAQLASQRGKVLLVVNTASKCGFTP
                     QFAGLESLWEQYRDRGLVVIGFPSNQFGSQDPGSNDEIASFCQLNYGVTFPMMAKVDV
                     NGEHAHPLWKWLTAEAPGILGTKAIKWNFTKFLVGRNGQVIKRYAPADSPASLKADIE
                     AALAQ"
BASE COUNT          569 a         1507 c         1400 g          674 t
ORIGIN      
        1 tcgcgggcac gcgggccttg cgcaggtgca cgggctcgcg ggcgtcgcgc cggcgcacgg
       61 gctcgcgcac cttcacggcg tcggcgcacc gaccgcgcga accggaacgc ggggcgcccg
      121 cgcgcacatc agctcgtagc cgatcgtccc ggcggcggcg gcgacctcgt cgatcggcag
      181 gacgtcgccg gaagcggcgc ggccccatag cacgacctcg ctgccgacgc cggcgcgggg
      241 agccggcgtc aggtcgaccg tgatcatgtc catcgacacg cgaccgacga tgcgcgtgcg
      301 aacgccatcg acgagaacgg gcgtgccgcg gtcgttgccg cccggcgcga cgcgcggata
      361 gccgtcggcg tagccgcagg cgacgacgcc gatccgcatc ggccgttcgg caacgtagcg
      421 cgaaccgtag ccgatgccag cgccggcggc cacgtcctgc acggcgatca gctcgctcct
      481 gagcgtcatc gccggctgca gcccccacgc ggccgcggtg tgcaacgggt gatcgggcga
      541 cgagccgtag agcatgatcc ccggccggac ccagtcaccg gcccgcgcgg cgcccagccg
      601 cagcgtcgcc gcgctgttgc agaccgaacg ctcgcccggg agctcggccg tcgcctcggc
      661 gaagaccgcg agcgccgcct cgagcgacgc ggtgtccgac gcatcggcgt tggccagatg
      721 cgtcatgagc gtgatcgcat cgacctgcgg cagcgcgtcc aggcgcagcc acgcggcccg
      781 gtaggcgtcg acgccgaagc ccagccggtt catgccgctg ttgagcttga ggaagatgcg
      841 gtgcgcatgc tcggtcttgt gcgcggccag ccagtcgatc tgcgccgttc gatgcaccgt
      901 gtgccagagg ttcaggcgtg aacacgcgtc gaggtcgcgc gggccgaagc atccctcgag
      961 caggaggatc gcgccgcgcc agccgaggtc gcgcagccgt tcggcttcgg ccaggtcgag
     1021 cagcgcgaag ccatcggcgc ctttcaggcc cgcataagcc tggtcgatat cgtggccgta
     1081 ggcgtcggcc ttgaccacgg cccagactct ggcgccgcct gcggcacggc gagcgacagc
     1141 gaggttggcc tcgagagcgt cgagatggac gagcgcttcg atcggccgcg gcatggcggg
     1201 aattctgcca gtcgcgatcg gcctccgcgg tcgcggcgaa ggcccgaaaa cccgcgtgct
     1261 ataaaccccg gcccccggaa aaccggggcg gatgcgatcc gccccgcgcc gctgcgaagc
     1321 aatgaaaaaa ggcttctact ccatcatggc ggcgcagttc ttcagctcgc tggcggacaa
     1381 cgcgttgctc gtcgccgcca tcgagctgct gaggaccggc ggcgcgccgt cgtggcacat
     1441 ccctgcgctg acgccgattg ccgcactctt ctacgtgctc ctcgcgccgg tcgtgggcgc
     1501 ctttgccgac gccgtgccca agggcatcgt catgttcgtc tcgaacgcga tcaaggtgat
     1561 cggctgcctg ctcatcctgt tcggcgccga cccgctgctc tcctacggcg tcgtcggcct
     1621 gggcgcggcg gcttattcgc cggccaagta cggcatcctc accgagctgc tgccgaattc
     1681 gcaactcgtc aaggccaacg gctggatcga aggcctgacg atcctgtcga tcatcctcgg
     1741 cgtgctgctc ggcggccagc tgctgggcca cgtcgtctcg cagcaattgc tttcgttcga
     1801 catgcccctc atcgataccg gcatctcgac cgcgccggag gcggcggtcg cggtgatcgt
     1861 cgcgatctac gtcgtcgcgg ccctcttcaa cctgcgcatc ccgcgcaccg aggcgccgct
     1921 gcagccgctg tcttcgagca tcggcgcgct gatccgcgac ttctcgaact gcaacgcgcg
     1981 gctctgggcc gacaagctcg gccagatctc gctggcgacg acgacgttgt tctggggcgt
     2041 ctccggcaat cttcgcgtca tcgtcttcgc ctgggcggcg atcgcgctcg gctactccac
     2101 gaaggacgct tcctcgctgg tcggcgtcgt cgcgatcggc accgccgccg gcgccatcct
     2161 ggcgtcgatg cggatgcggc tcgaccaggc gacacgggtc atcccgctcg gcatcgccat
     2221 gggcgtgctc gtgatcggcc tcgacgtgat ccgcagcccg tggatcgcgg cgccctttct
     2281 cgtcgtcctc ggcgccatcg gcggctacct cgtcgtaccg atgaacgcgc tgctgcagca
     2341 ccggggtgcc aacctgatgg gcgccggccg ttccatcgcc gtgcagaact tcaacgagca
     2401 ggcgtgcatc ctcggcctcg gcgccttcta taccgggatg acacgcttcg gcctgtcggc
     2461 gttcacggcg atcggcgtct tcggcgttct cgtcacggcg atgatgtgga tgatccggcg
     2521 ctggcacgcc accaactgcg tccggtatcc cgaggagatc gcccatctcc tctacatcgc
     2581 ccggctcgac aagcattgaa cgacgcgtcg ccaacgtcat cccggcagac cctgctcgcc
     2641 agtgccgcgc tggtgttcaa cgccttggtc tggggcacgt cgtggtggcc gtttcgaaag
     2701 ctgcacgacg ccggcctgca tccgctctgg gccaccgcga tcgtcttcgg cgtggcctcg
     2761 ctggtcatcg tcgcggcgcg gccgcgcgcc ctggcgcaag tgctgcgcac gccggcgctg
     2821 tggctgctcg tcgccgcggc gggcacaacc aacgccgcct tcaactgggc cgtcgtcatc
     2881 ggcgacgtcg tccgggtggt cctgctcttc tacctgatgc cgctgtggac ggtcctgctc
     2941 gcccgcgtcg tcctcggcga aaggctgtcg gccgcggcgg cgcttcgcat cgccctcgcc
     3001 ctggtcgggg cggcgatcgt gctctggccg ccgtcgacgg gcacggcgcc ttcctcctgg
     3061 gcggagcgct tgccgttgcc gcgctcgctg gccgactggc tgggcatcgt cggcggattc
     3121 tcgttcgcct tgaacaacgt gatgcttcgc cgggaagcgg cccagccgga agaaggccgg
     3181 gcgctggcga tgttcatcgg cggcgccgtc gtcgccggca tcctcgcgtc ggtgctcacc
     3241 gcgtcgggtg cgctcgctgc gccgccgccg tcggccgcgt ggctatggcc ggcagcggcg
     3301 ctgacgacgc tctttctcgg cagcaacctg gcgctgcagt acggcgcggc acggttgccg
     3361 gccaatcgca cctcggtcgt gatgctcacc gaagtcgtct tcgcgtcggc gtcgtcggtg
     3421 gccttcggcg gtgccgagcc gacggcgcgg accgtgctcg gcgggggcct gatcgtggcg
     3481 agcgccgtct tcgctgcgct cgcgccgtcg cgctgaaccg cgccgcgagc ggcccggcgg
     3541 taccatgggc gttgcttctg atcgccggag ggcgccatgg ccgacacgcc gcacagcatc
     3601 tacgacttcg aggcgccctc gatccagggc cagacggcgc agctcgcaag ccagcgcggc
     3661 aaggtgctgc tggtcgtcaa cacggccagc aaatgcggct tcacgccgca gttcgccggc
     3721 ctcgaatcgc tctgggagca ataccgcgac cgcggcctcg tcgtcatcgg tttcccgagc
     3781 aaccagttcg gttcgcagga ccctggctcg aacgacgaga tcgcctcgtt ctgccagctc
     3841 aactacggcg tcacgtttcc gatgatggcc aaggtcgacg tcaacggcga gcacgcccat
     3901 ccgctgtgga agtggctcac cgccgaagcg ccgggcatcc tcggcaccaa ggccatcaag
     3961 tggaacttca cgaagttcct cgtcggcagg aacggacagg tgatcaagcg ctatgcaccc
     4021 gccgattcgc cggcctcgct caaggccgac atcgaagccg cgctcgccca atgaccggcc
     4081 ccgctcgcct ccggccggca cgccgggtcg cgaacccgcc ggcgacccac caggacgaca
     4141 gccatgtttg
//
LOCUS       DASRWS010000060         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1464915, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000060 DASRWS010000000
VERSION     DASRWS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464915"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      42..129
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      146..251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            301..1452
                     /gene="gcvT"
                     /locus_tag="VFF72_01070"
     CDS             301..1452
                     /gene="gcvT"
                     /locus_tag="VFF72_01070"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZV91772.1"
                     /translation="MADPSSPSPAALSRTPLHALHRELGAKMVPFAGHEMPVSYPAGI
                     LAEHRACRESAALFDVSHMGQILVSGADAAAALETLWPVDLAGLAAGRQRYAFFTDAA
                     GGILDDLMATRRDDDGCSGFHVVVNAGNKGDDLARLQAAVGTRCRIEERFDRALLALQ
                     GPRAVDVMTRLAPSLAALTFMTGAMATIRGIDVYATRSGYTGEDGWEISVAADDAERL
                     ARVLLAEPEVQAAGLGARDTLRLEAGLCLHGHDIDPTTTPVEAGLSWAIQKVRRAGGA
                     RAGGYPGADTIDHQLAEGTPRKRVGLVGDDRAPVREGATLHLGDDARVVGRVTSGTLA
                     PTLGRPVAMAYVDTAHAATGTLLHADVRGRRLPMRVAPMPFVAHRYFRG"
     gene            1484..1855
                     /gene="gcvH"
                     /locus_tag="VFF72_01075"
     CDS             1484..1855
                     /gene="gcvH"
                     /locus_tag="VFF72_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZV91773.1"
                     /translation="MTTYYTSDHEWISGDAASATVGITTHAQDALGDVVFVDLPAVGK
                     SFRKGEVAGVVESVKAAADLYMPVAGEIVEVNEALRADPSLANSDPQAAGWFFKVRLA
                     DPSELSGLLDQAAYDELVKSA"
BASE COUNT          293 a          782 c          685 g          268 t
ORIGIN      
        1 ccgcgcgact ccgcacgagg gcgcgcggta gactactccc cgccaggaga gcgctgcacg
       61 caaatgcggc cgccgaaggc gcagaccgcc gcgcaaccgc ggtcgaacgc tcaggcaaag
      121 aggactggca cccgcctgct cgcaggcggt tctggctgga gagaggcctg ccccgagcag
      181 gcccaccgaa ggggcaagcg gcatcgcgag gtgccgccaa tctctcaggt agagcggaca
      241 gccggcaatt cgagccgcga accctggttc gcggatgcgt gaattgctcg gagtccgtcc
      301 atggccgacc cgtcgtcccc ctccccggcc gcgctgtcgc gaacgccgct gcacgcgctg
      361 catcgtgagc tcggcgccaa gatggttccg ttcgccggcc acgagatgcc ggtctcctac
      421 ccggccggca tcctcgccga gcaccgcgcc tgtcgcgaat cggcggcctt gttcgacgtc
      481 tcgcacatgg ggcagatcct cgtgtcgggc gccgacgccg cggccgcgct ggaaacactg
      541 tggccggtcg acctcgccgg ccttgccgcc ggcaggcagc gctacgcctt cttcaccgac
      601 gccgcgggcg ggatcctcga cgatctgatg gcgacgcgcc gcgacgacga cggctgctcg
      661 ggcttccacg tcgtcgtcaa cgccggcaac aagggcgatg accttgcgcg cctccaggcg
      721 gcagtcggca cgcgctgccg tatcgaggag cgcttcgacc gcgcgctgct cgcgctgcag
      781 ggcccgcgcg cggtcgacgt gatgacgcgc ctcgcgccct cgctcgccgc ccttaccttc
      841 atgaccggcg ccatggcgac catccgcggc atcgacgtct acgcgacgcg ctccggctac
      901 accggcgagg acggttggga gatctcggtc gcggccgacg acgccgagcg gctcgcccgc
      961 gtcctcctcg cggaacccga agtgcaggcg gccggcctcg gcgcgcgcga cacgctgcgc
     1021 ctcgaagcgg gcctgtgcct gcacggccac gacatcgacc cgaccaccac gcccgtcgag
     1081 gccggcctct cgtgggcgat ccagaaggtc cgccgcgccg gtggcgcgcg ggctggcggc
     1141 tacccgggcg ccgacacgat cgaccaccag ctcgccgaag gcacgccccg caagcgcgtc
     1201 ggcctcgtcg gcgacgaccg cgccccggtg cgcgaaggtg cgacgctgca cctcggcgac
     1261 gacgcccgcg tggtcggccg cgtcaccagc ggcacgctcg cgccgacgct cgggcggccg
     1321 gtggcgatgg cgtacgtcga caccgcgcac gccgcgaccg gcacgctgct gcacgccgac
     1381 gtgcgcggcc gccgcctgcc gatgcgcgtc gccccgatgc ccttcgtggc ccatcgctac
     1441 ttccgcggct gaacgtcatc cgatccacca ccgagcgacc tccatgacca cgtactacac
     1501 ctccgaccac gaatggattt ccggcgacgc cgcaagcgcc acggtcggca tcaccaccca
     1561 cgcccaggac gccctcggcg acgtcgtctt cgtcgatctg ccggcggtcg gcaagtcgtt
     1621 ccggaagggc gaggtcgcgg gcgtcgtcga gtcggtcaag gcggccgccg atctctacat
     1681 gccggtcgcc ggcgagatcg tcgaggtgaa cgaggcactg cgcgccgacc cgtcgctcgc
     1741 gaacagcgac ccgcaggccg ccgggtggtt cttcaaggtc aggcttgccg atccctcgga
     1801 gctgagtggc ctgttggatc aggccgccta cgacgagctc gtgaagagcg cctgaccgcg
     1861 gaccgactgc catgctgatg cccgccgcga agccgctcgc cgagctcgag aaccccgccg
     1921 agttcgtcgc ccgccacatc ggcatcggcg aggccgacga gccgcgcatg ctggcctcga
     1981 tcggagtcga atcgcgccag gccctgatcg acgccgtcgt gcccgcgt
//
LOCUS       DASRWS010000061         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4056403, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000061 DASRWS010000000
VERSION     DASRWS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056403"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..365)
                     /locus_tag="VFF72_01080"
     CDS             complement(174..365)
                     /locus_tag="VFF72_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91774.1"
                     /translation="MKPAHLARPYQESRIEDEPILGPVLVLPNGYVQPLSFVERLLVT
                     LHLTNAKSLEARYLKAAQS"
     gene            complement(451..693)
                     /locus_tag="VFF72_01085"
     CDS             complement(451..693)
                     /locus_tag="VFF72_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91775.1"
                     /translation="MTHARSGVCALAVLMLAACAASQPPTTTEAQAQSRCERVSRTGS
                     NLPVLQCRPGATEDERQQDIDDWGRSTHGRRSSGGG"
     gene            814..1974
                     /locus_tag="VFF72_01090"
     CDS             814..1974
                     /locus_tag="VFF72_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HZV91776.1"
                     /translation="MKCVLVGSRFFGASVFEALRQEDGVAFVAVVAPAADDRLALAAQ
                     KAGVPVHVLADPKIVPGEAVPAGSDLVVAAHTHARVSNEALAHARLGGIGYHPSLLPR
                     HRGIAAVEWTILEGDAIAGGSVYHLADGWDAGAVAAQDWCFVGKGETARELWERALAP
                     MGLALLARVVRHARDEGRLPAYAQDPRFATRAPMIRRSVVLKDEPAPATTSLVVTVMG
                     TDRPGIVRQLSDRAQRFGANWAASRFSRLAGEFAGMVHFEVPGSSADALAESLRGLES
                     AGLRLVIVRSDATQAPGSQRGVELDLIGEDRPGIVARLTAILADAGVSIEHIETEIVG
                     HDTGKKTFKVSAHLRVPRTLSDDDLRGQLGEVAHEMMLDVNLGEREEGVSRP"
     gene            2136..3023
                     /locus_tag="VFF72_01095"
     CDS             2136..3023
                     /locus_tag="VFF72_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZV91777.1"
                     /translation="MPVIAVVNRKGGCGKSTLATHLAAWLAGQGHAVMLGDVDRQQST
                     QGWLRARAEHALPGVPIVGWAVDPRRVLRPPAGIGFVVLDTPGGLHGFDLARVVAFAD
                     VILMPVCQSRFDRESAADCHAELAALPRVAGGRCKVAAIGMRLDARTRAAQALTDWAR
                     ELRLPFIGALREAQVYVRCIERGLTLFDLPAQQSAADRAQWQSIFDWLDPALSGVPRP
                     QIRGNVRVVRPETHESSGAAADPGRIWRSAPPLAPAPASPRRSTAVSTVRAPAAGIAR
                     WLSRLGWLSPSPGFLNRHS"
     gene            3059..>3848
                     /locus_tag="VFF72_01100"
     CDS             3059..>3848
                     /locus_tag="VFF72_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HZV91778.1"
                     /translation="MAKTPAAKNPAHQRADLVRIATQAMLDRGLEPEFSAEALLQLAS
                     IKGPGSDADGSIRDLTSLPWCSIDNDDSRDLDQLTACEALERGAVRILVAIADVDAVV
                     GKGSPLDAHAWANTTSVYTSARVFPMLPERLSTDLTSLNADADRLALVTEFRVAADAS
                     VSDATVYRARVRNRAKLAYDAVSAWLDGTGPMPAPVRLAAGMDEQLRMQDGVAQRLRA
                     QRHAAGSLEFETLQPHAIFDGDRVVDIREQPHNRGRQLIEELMIV"
BASE COUNT          525 a         1393 c         1326 g          604 t
ORIGIN      
        1 cctgccttcc acgccatgac gatctccttg ccgaggaaga ccatcctatg cagcggctgt
       61 gtcgctgcac ggcaggcgcc gcgaccgacg tgacttccgc acgcgcggag gcgctgccct
      121 ttgtcacatc cgaccgaaga cgcggcggct gatcgccgca cccgccggcg ggatcaggac
      181 tgcgcggcct tcaggtagcg cgcttcgagc gacttggcat tcgtcaggtg cagcgtcacc
      241 agcagccgct cgacgaacga cagcggctgg acatagccgt tcggcaacac gaggacgggg
      301 ccgagaatgg gttcgtcctc gatcctcgac tcctgatagg gacgcgcgag atgagctggc
      361 ttcatgctgt ctccttgcgg ccgccgacgc cggccatggc gcaatgtaac cgaatgttca
      421 tctcgctcga cacccggcgc cagccgttcg tcaaccgccg ccgctggacc tgcgaccgtg
      481 cgtcgatcgg ccccagtcat cgatgtcctg ctgccgctcg tcttccgtgg cgccggggcg
      541 gcattgcagc acgggcaggt tcgagccggt tcggctgacg cgctcgcagc gggactgcgc
      601 ctgcgcttct gtggtggtgg gcggctgcga ggccgcacac gcagcaagca tcaggacggc
      661 cagcgcgcat acgccggatc tggcatgggt catcgtttcc tccctgcggc atcgtctacg
      721 cttcggcaat ggccggcgct taccgcgccg agcagtctag ccgcccggaa tcgcgccacg
      781 cggcggcgcc cgctcgatga aggaactgtc tcgatgaaat gcgttctggt cggttcacgc
      841 tttttcggcg cctcggtgtt cgaggcgctg cgccaggagg acggcgtcgc gttcgtcgcc
      901 gtcgtcgcgc ccgcggccga tgaccggctc gcgctcgccg cgcagaaggc gggcgttccg
      961 gtgcacgttc ttgccgatcc gaagatcgtc cccggcgagg ccgtgcccgc aggtagcgac
     1021 cttgtcgtcg ccgcgcacac gcatgcccgt gtcagcaacg aggcgctggc gcacgcacgc
     1081 ctcggcggca tcggctatca cccgtcgctg ttgccgcggc accgcggcat cgctgccgtc
     1141 gaatggacca tcctcgaagg cgacgcgatc gccggcggct cggtgtatca ccttgccgac
     1201 ggctgggacg ccggcgcggt cgccgcgcag gactggtgct tcgtcggcaa gggcgagacg
     1261 gcgcgcgagc tgtgggagcg ggcgctggcg ccgatgggcc tggcgcttct cgcccgggtg
     1321 gtgcgccacg cgcgcgacga ggggcgcctg ccggcctacg cccaggatcc gcgcttcgcg
     1381 accagggcgc cgatgattcg ccgctcggtc gtgctcaagg acgagccggc gccggcgacg
     1441 acgtcgctcg tcgtcacggt catggggacg gaccggccgg gcatcgtccg ccagctctcc
     1501 gatcgtgcgc agcgcttcgg tgcgaactgg gccgccagcc ggttctcgcg cctcgcgggc
     1561 gagttcgcgg ggatggtcca tttcgaggtg ccgggcagca gcgccgacgc cctggccgag
     1621 tcgctgcgcg gtctcgaatc ggcgggcttg cggctggtca tcgtcaggag cgacgcgacg
     1681 caggcgccgg gcagccagcg cggcgtcgag ctcgacctga tcggcgagga ccgtcccggc
     1741 atcgttgccc ggctgaccgc gatcctcgcc gacgcgggcg tcagcatcga gcacatcgaa
     1801 accgagatcg tcggccacga caccggcaag aagacgttca aggtgtcggc gcacctgcgc
     1861 gtgccgcgaa cgctttcgga cgacgacctg cgcggccaac tcggcgaagt ggcgcacgag
     1921 atgatgctcg acgtcaacct cggcgaacga gaagaaggcg tttcgaggcc ctgatcgtcg
     1981 ctttgccgac tcgcggccct gtttcaatcg gacaagcctt ctcgcaggag cgacgggtgg
     2041 cgcatcggca tccggccggc ccgccgcctc gtccgcgccg cgcgccggcc gcaacgccat
     2101 cgccttcagc ccagccttcg cctgctttcg tcgccatgcc cgtcatcgcc gtcgtcaatc
     2161 gcaagggagg ctgcggcaag agcaccctgg cgacgcatct cgcggcctgg ctggccggcc
     2221 aggggcacgc ggtcatgctg ggtgacgtcg accgccagca gtcgacccag ggctggctgc
     2281 gtgcgcgcgc ggaacacgcc ctgccaggcg ttccgatcgt cggctgggcc gtcgacccgc
     2341 ggcgcgtgct gcgacctccg gcgggcatcg gcttcgtcgt cctcgacacg ccgggaggcc
     2401 tccatggctt cgatctcgcc cgggtcgtcg cctttgccga tgtcatcctg atgccggtct
     2461 gccaatcgag attcgaccgt gaatcggcgg ccgactgcca cgccgagctc gcggcgctgc
     2521 cgcgcgttgc cggcggtcgc tgcaaggtcg cggcgatcgg catgcgcctg gacgcgcgca
     2581 cccgggccgc gcaggcgctc accgactggg cgcgagagct ccggctgcca ttcatcggcg
     2641 ccttgcgcga ggcgcaggtc tacgtgcgct gcatcgagcg cggcctgacg ctgttcgacc
     2701 tgcctgccca gcagtccgcg gccgatcgcg cccagtggca atcgatcttc gactggctcg
     2761 atccggcgct gagcggcgtg ccacggccgc agatccgcgg caacgtgcgt gtcgtgcggc
     2821 ccgagaccca cgaatcgagc ggcgctgcgg ccgatcccgg gcgcatctgg cggtccgcgc
     2881 cgcccctggc gccggcccct gcgagcccgc gccggtcgac ggcggtttcg accgtccgcg
     2941 cgccggcggc gggcatcgcc cgctggctca gtcgcctcgg ctggttgtcg ccttcgccgg
     3001 gattcctgaa ccggcactcc tgaggccggc gcgttgccgg cctgggctac attggctgat
     3061 ggccaagact ccagcggcga agaaccctgc acaccagcgt gccgacctcg tgcgcatcgc
     3121 cacccaggcg atgctcgacc ggggcctgga gcctgaattc tcggccgagg cgctgttgca
     3181 attggcgtcg atcaaggggc ccggcagcga cgccgacggg tcgatccgcg acctgacgtc
     3241 gttgccgtgg tgctcgatcg acaacgacga ctcgcgcgac ctcgaccagc tcaccgcctg
     3301 cgaggcgctc gagcgcggcg cggtgcgcat cctcgtcgcg atcgccgacg tcgacgccgt
     3361 cgtcggcaag ggctcgccgc tcgacgcgca tgcctgggcc aacacgacct ccgtgtacac
     3421 gtcggcccgc gtctttccga tgctgcccga gcgcttgtcg accgacctga cgtcgctcaa
     3481 cgccgacgcc gatcgactcg cgctcgtgac cgaattcaga gtcgccgccg atgcatcggt
     3541 cagcgacgcg accgtctatc gcgcgcgcgt tcgaaaccgg gccaagctcg cctacgacgc
     3601 cgtctcggca tggctcgacg gcaccggccc gatgcccgcg ccggttcgtc ttgccgccgg
     3661 catggacgag caactgcgca tgcaggatgg cgttgcgcag cgtctgcgcg cccagcggca
     3721 tgccgcgggc tcgctcgagt tcgagacgct gcaaccgcac gccatcttcg acggcgatcg
     3781 cgtcgtcgac atccgggagc agccgcacaa ccgcggccgc cagctcatcg aggagctgat
     3841 gatcgtta
//
LOCUS       DASRWS010000062         7464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_604974, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000062 DASRWS010000000
VERSION     DASRWS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604974"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFF72_01105"
     CDS             complement(<1..386)
                     /locus_tag="VFF72_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV91779.1"
                     /translation="MGDSRTSAAPAEIALLQNHSLAMLVQRELERMVLSGKLVAGERL
                     NEPVLATRLGVSRGPVREAIRTLAEKGLVRIEKNRGTFVREISVGEADEIFEVRASLD
                     RLAGRKVAGSIGAEHLAQLRDMVGEM"
     gene            complement(654..908)
                     /locus_tag="VFF72_01110"
     CDS             complement(654..908)
                     /locus_tag="VFF72_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91780.1"
                     /translation="MDLPAAPVCPECGFRVFNRRYPKCESCGAILPETLVYSATERHA
                     LRVADEERELERARHERPSDSILPPAEEHPLLSAMLDLAE"
     gene            complement(1031..1759)
                     /locus_tag="VFF72_01115"
     CDS             complement(1031..1759)
                     /locus_tag="VFF72_01115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV91781.1"
                     /translation="MTQDRGMLSFVVPAHDEEASIDAVVRAIATAATGTSHEIVVVDD
                     ASTDRTAELARRAGATVVSIDRRQIAAARNAGAQQATGDVLVFVDADTLVDAAVVAGL
                     CRAIDEGAVGGGAAIRFDEPVPRWVTWILPVASWIGRRMRVTGGCFVFCRRVDFEAIG
                     GFDETLFAAEEIALCRAMARRGRVAILRESVLTSGRKLRTYTGRELLAVCLHVAFAGR
                     AGLRDRSRLGVWYDARRPDRRGPS"
     gene            complement(1830..3986)
                     /locus_tag="VFF72_01120"
     CDS             complement(1830..3986)
                     /locus_tag="VFF72_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HZV91782.1"
                     /translation="MNRRPWLPAPLRPWRLLPAYAINGIEAALGIGCIQLLAHAVFGS
                     HVAALVVSGAVCASIADLPNVLKRTWYRVGTAALLAFIAAVVVDLLRHDPRVLGLAVG
                     AVSFCAMMALAWGARAGAVSFAPVLSMIFSMAVPPAEHELAVAGWGACGGFAYLAWAL
                     AANAVLQGRYRSLVLANTLRAAAVLFRSRADVLVSFRVEAGEAPPLRAWISGEAALAD
                     RLQAARDLLYPAPESPRWRRDVAILLHVIDMRDALLASRLDVELLDGDAAGRSVLELA
                     AQALRDVGAMLDAAADALRDGVFDRAVPAFASVSFAAAPSGTTLPGDARARLLPVLEG
                     RLGRLADVVTRIHRLLGGRSEPLPLSHAELQRFVAPEGWPIRALRSHWRRDSLVLRHA
                     ARMSLAMCAAYYIALALPWGSHPYWLVLSVAVVLRGNFGDTLARRNARVLGTLLGCLV
                     VVGLARLSPSLQGGIFVVALGIAHTFVIQRYWLTATAASVMALLQSHLVNPATGFAIA
                     ERVGDTLLGAVMAWSFSYVLPSWERRSVPEAIGRVLRELREYAAHSLHAGSGDAVDER
                     MARRRAYESLAALGAALQRSRVEPKGVRLSVRQIAAFLDHGERFMAHLSLVRHSLARL
                     ADAAELPRIEAALAETVAGLGRRLDLDMPAGPAPEHPADDAALDLLPAQPPAQDRAAW
                     LARRLSQLQDEAARIRAAADASLALPAWKYTASASL"
     gene            complement(3983..4846)
                     /locus_tag="VFF72_01125"
     CDS             complement(3983..4846)
                     /locus_tag="VFF72_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZV91783.1"
                     /translation="MKLMRYGAKGAEKPALVDTHGTVRDLSGTISDITAATLAPANLA
                     KLAALDATRLAAVAHPGRIAPPWSGMGKFICIGLNYSDHAAETGSPIPKEPIIFMKPT
                     SALVGCNDPVVMPRDSVKTDWEVELGVVIGTKARYVDQAEALKYVAGYCVVNDVSERE
                     YQLERGTQWDKGKGCDTFGPVGPWMVTADEVADPQALGLWLEVNGKRVQNGSTKTMIF
                     GVAELVAYCSRFMTLWPGDLITTGTPPGVGLGMKPPRYLKAGDEMRLGIDGLGEQRQK
                     VHAWDPKLIDD"
     gene            complement(4877..5641)
                     /locus_tag="VFF72_01130"
     CDS             complement(4877..5641)
                     /locus_tag="VFF72_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV91784.1"
                     /translation="MTDRLAGKTAFITAAGQGIGRATAEAFVREGARVIATDIDDSLL
                     DDLRARTGCETRRLDVTDASAVLAASIEALARGPVHVLFNGAGFVHAGTVLDCDEVAF
                     DFSFDLNVRSMYRLIRAFLPSMLEHGNASIINVASVVGSIKGAPNRFVYGTTKAAVIG
                     LTKAVAVDFVTRGVRCNAICPGTVESPSLGERIKAQARASGNSEAVVRDGFVGRQPMG
                     RLGRPDEIAALAVYLASDESAFTTGTAQVIDGGWSN"
     gene            5902..7317
                     /locus_tag="VFF72_01135"
     CDS             5902..7317
                     /locus_tag="VFF72_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV91785.1"
                     /translation="MDQPPDLLPLAPSPPPLLLPPPDRPRRSRWLGGIVAVLALLALG
                     ALAWYLTHRPAASPGGFGPAAAASGASGAAARPVGAPGGRGGRFGAASTVGIAAAKRA
                     DLPVYIDALGTVLAAVTTTVRPQVSGVITRIAYTEGQMVKKGQLLATIDPRSFEIALQ
                     QAIGARMRDEATLQSNKVQLQRYQTLLSQDSIARQDVDTLAATVKQLEGTIVIDRANE
                     STARLNLSYCRIVAPIDGRVGLRPIDQGNYIAAGDTNGVAVITQVEPINVEFAVPQDL
                     IPQIRSRVASGAKLAVTAFDRTRTVKLDEGTFSTLDNQIDTQTGTVKAKARFANTARQ
                     LFPNQFVNVRLLLQVDQGAIVIPVTAIRTGPNGDFVYVLNDDRTVSVRPVTRGIATTN
                     LVAIDKGLEAGEQVVTEGGDRLKDGARVQLAADLPPAGASAAARAHGARRGASAAEGA
                     ASGHRRRSLDGIPAAASAPAS"
BASE COUNT         1190 a         2641 c         2615 g         1018 t
ORIGIN      
        1 tccatctcgc cgaccatgtc gcgcaattgc gccagatgtt cggcgccgat cgaacccgcg
       61 accttgcgac cggccagtcg gtcgagcgac gcacgcacct cgaagatctc gtcggcctcg
      121 cccaccgaga tctcccgcac gaaagtgcct cggttcttct cgatgcgaac gaggcccttt
      181 tccgccagcg tccgtatggc ttcgcgcacc gggccgcgcg agacacccag gcgtgtcgcc
      241 agcaccggct cgttcagcct ctcgccggcg accagcttgc ccgacaggac cattcgctcc
      301 agctcgcgct ggaccagcat cgccagggag tggttctgca gcagggcgat ttcggcaggg
      361 gcggcgctgg tacggctgtc tcccatgcgc gatttttgat agtcgacgaa agattgtcaa
      421 caatatttta tcggtgcatt cgctgtctgg ctcctcttgc cgcggcaagg ccgggatgcg
      481 aattggcccc tcgccggccc ctggacacgt gggtcgccag ccggcaaagg ttgcgcggcg
      541 acgaagaaaa ggtcacctcc gcctgcgccg gcgtggcgac gaatcgggca tggccggcag
      601 actcggggac gcggggtggc gatgccgccc cgatccgtcg ctcgatcgcg cgcctattcc
      661 gcgaggtcga gcatcgccga gagcagggga tgctcttcgg ccggaggcag gatggagtcg
      721 ctcggccgtt cgtggcgggc ccgttcgagt tcgcgctcct cgtcggcgac ccgcagtgcg
      781 tgacgctcgg ttgcggaata gaccagcgtc tcgggcagga tcgcgccgca cgattcgcac
      841 ttcggatagc ggcgattgaa gacgcgaaag ccgcattcgg gacaaacagg cgcggcgggc
      901 agatccattg ttcgctccgg cagttctcgt cagccgcaac ttagcgcgac cgcgccaggc
      961 accggggtcg gagacgatcg cggctcgccg taggacgcgt cgccccggcc ggcggccctc
     1021 ggttcgccgg ctacgaaggc ccgcgacgat cggggcgtcg cgcgtcgtac cagacgccca
     1081 gtcgcgaacg atcgcgcagg ccggcgcgac cggcgaaagc cacgtgcagg cacacagcca
     1141 ggagctcgcg tcccgtgtac gtgcgaagct ttcgcccgga agtcagcacc gactcgcgaa
     1201 gaatcgccac cctgccacgc cgagccatcg cacggcacag ggcgatctcc tcggccgcga
     1261 acagggtctc gtcgaagccg ccgatcgcct cgaagtcgac gcgcctgcaa aagacgaagc
     1321 agccgccggt cacgcgcatg cggcggccga tccacgaggc caccggcaag atccacgtca
     1381 cccagcgcgg caccggctcg tcgaagcgga tggcagcgcc accgccgacg gcgccttcgt
     1441 cgatggcccg gcaaagtccg gcgacgacag cggcatcgac caaggtgtct gcgtcgacga
     1501 agacgagcac gtcgcccgtc gcctgctgcg cgccggcgtt gcgcgcggcg gcgatctgcc
     1561 gccggtcgat cgacaccacc gtcgcgccgg cgcggcgggc gagctccgcg gtgcggtctg
     1621 tcgacgcgtc gtcgacgacg acgatctcgt gcgaggtgcc ggtggctgcc gtggcgatgg
     1681 cgcgaacgac cgcgtcgatc gacgcctcct cgtcgtgcgc cggaacgacg aacgacaaca
     1741 tcccgcgatc ctgcgtcatg gccagcggcc ctcgacgacc aggctgcgcc acgtgccgca
     1801 acaacaccgc ggccgccgca cggcgcgcgc tacaggctgg cggaggccgt gtatttccac
     1861 gccggcaggg ccaggctcgc atccgcggcg gcacgaatgc gcgcagcttc gtcctgaagc
     1921 tgcgacagcc gccttgccag ccaggccgcc cgatcctgcg ccggcggctg cgccggcagc
     1981 aggtcgagcg ccgcatcgtc ggcggggtgt tcgggtgcgg ggccggcggg catgtcgaga
     2041 tcgaggcgcc gaccgaggcc cgcgaccgtc tcggcaagcg ccgcttcgat gcgcggcagc
     2101 tcggccgcgt cggcaaggcg cgccagcgag tggcgcacga gcgaaagatg cgccatgaag
     2161 cgctcgccgt ggtcgaggaa ggcggcgatc tgccgcaccg acaggcgcac gcccttgggt
     2221 tcgacccggc tgcgctgcag cgcggcgccg agcgcggcca gcgactcgta ggcacggcgc
     2281 cgcgccatcc gctcgtcgac ggcgtcgccc gatccggcgt gcagcgaatg cgcggcgtac
     2341 tcgcgcagct cgcgaagcac ccggccgatg gcttcgggca cgctgcgccg ctcccacgac
     2401 ggcagcacgt agctgaacga ccaggccatg accgcaccga gcagggtgtc gccgacacgc
     2461 tcggcaatgg caaagccggt ggccgggttg acgaggtgcg actgcagcag cgccatgacg
     2521 ctggccgcgg ttgcggtcag ccagtagcgc tggatgacga aggtgtgcgc gatgccgagg
     2581 gcgacgacga agatgccgcc ctgaagcgaa ggcgacaggc gcgccaggcc gaccacgacg
     2641 aggcaaccga gcaaggtgcc gaggacccgg gcgttgcgcc tcgccagcgt atcgccgaag
     2701 ttgccgcgca ggacgacggc gacgctgagg accagccagt acggatgcga tccccagggc
     2761 agcgccaggg cgatgtagta ggccgcgcac atcgccagcg acatccgggc ggcgtgacgg
     2821 agcaccagcg aatcgcgccg ccagtgcgag cgcaaggcgc gaatcggcca gccctcggga
     2881 gcgacgaagc gctgcagctc ggcatgcgag agcggcagcg gctcgctccg gccaccaaga
     2941 agccggtgga tgcgcgtcac cacgtcggcc agccggccga gccggccttc cagcaccggc
     3001 aggaggcggg cgcgcgcatc gccaggaagc gtcgtcccgg acggcgccgc ggcgaagctg
     3061 accgaggcga acgccggcac ggcgcggtcg aacacgccgt cgcgcaacgc gtcggccgcg
     3121 gcatcgagca tcgcgccgac gtcacgcaac gcctgcgcgg ccagctccag caccgagcgg
     3181 ccggcggcat cgccgtcgag cagctcgacg tcgaggcgac tggcgagcaa ggcgtcgcgc
     3241 atgtcgatga cgtgcagcag gatcgccacg tcgcggcgcc agcgcgggct ctccggcgcc
     3301 gggtagagca ggtcgcgcgc cgcctgcagg cgatcggcca gcgccgcctc gccgctgatc
     3361 caggcacgca gcggcggcgc ttcgccggcc tcgacgcgaa acgaaacgag cacgtcggca
     3421 cgcgagcgga acagcacggc cgcggcgcgc agcgtgttcg cgagcacgag gctgcgatag
     3481 cggccctgca gcaccgcatt ggcggcgagc gcccaggcga gataggcaaa gccgccgcat
     3541 gcaccccagc cggcgaccgc aagctcgtgc tcggccggcg gcaccgccat cgagaagatc
     3601 atcgaaagga ccggcgcgaa cgagaccgcg ccggcgcgcg caccccaggc gagcgccatc
     3661 atcgcgcaga agctgaccgc gccgacggcc aagccgagca cgcgaggatc gtggcggagc
     3721 aggtcgacga cgacggcggc gatgaaggcg agcagcgccg ccgtgccgac ccggtaccac
     3781 gtgcgcttca ggacattggg caggtcggca atgctggcgc agacggcgcc gctgacgacg
     3841 agcgcggcca catggctgcc gaacacggcg tgtgcgagca gctggatgca gccgatcccg
     3901 agggctgcct cgatgccgtt gatcgcatag gcgggcagca gccgccacgg ccgcagcggc
     3961 gccggcaacc agggccggcg attcaatcgt cgatcagctt cggatcccag gcatgcacct
     4021 tctgtcgctg ctcgccgagg ccgtcgatgc ccaggcgcat ctcgtcgccg gccttcaggt
     4081 acctgggcgg cttcatgccg aggccgacgc cgggcggcgt gccggtggtg atgagatcgc
     4141 cgggccacag cgtcatgaag cggctgcaat aggcgacgag ctcggcgacg ccgaagatca
     4201 tggtcttcgt cgagccgttc tggacgcgct tgccgttgac ttcgagccag aggccgagcg
     4261 cctgcgggtc ggcgacctcg tcggcggtca ccatccaggg gcccaccgga ccgaaggtgt
     4321 cgcagccctt gcccttgtcc cactgcgtgc cgcgctcgag ctggtactcg cgctcggaga
     4381 cgtcgttgac gacgcaatag ccggccacgt atttcagcgc ctcggcctgg tcgacgtagc
     4441 gcgccttggt gccgatgacg acgccgagct cgacctccca gtcggtcttg accgaatccc
     4501 tcggcatgac gaccgggtcg ttgcagccga ccagggcgct ggtcggcttc atgaagatga
     4561 tcggctcctt cgggatcggc gagccggtct cggcggcgtg atccgaataa ttgaggccga
     4621 tgcagatgaa cttgcccatg ccgctccagg gcggcgcgat ccgcccgggg tgggcaacgg
     4681 cggcaagcct ggtcgcgtcg agcgccgcca gcttggcgag gttggcggga gcgagcgtcg
     4741 cggccgtgat atcggagatc gttcccgaaa gatcgcgaac cgttccgtgc gtgtcgacga
     4801 gcgccggctt ttccgcgccc ttggcgccgt atcgcatcaa cttcatgtga gcccctgacc
     4861 gtgtgggagg aaagggctaa ttggaccatc ctccgtcgat cacctgcgcc gtcccggtgg
     4921 tgaacgcgga ttcgtcgctc gccaggtaca ccgccagcgc cgcgatctcg tcggggcgac
     4981 cgagccggcc catcggctgg cgaccgacga agccgtcgcg cacgaccgct tcgctgtttc
     5041 cgctcgcccg tgcctgcgcc ttgatgcgct cgcccagcga cggcgattcg accgtgcccg
     5101 ggcagatcgc gttgcaacgg acgccccggg tgacgaagtc gacggcaacg gccttggtga
     5161 ggccgatcac cgcagccttg gtggtgccgt agacaaagcg gttgggtgcg cccttgatgc
     5221 tgccgaccac cgaggccacg ttgatgatcg acgcgttgcc gtgctcgagc atcgacggca
     5281 ggaaggcgcg gatcagccgg tacatcgaac gcacgttgag gtcgaaggag aagtcgaacg
     5341 cgacctcgtc gcaatcgagc accgtgccgg cgtgcacgaa tccggcgccg ttgaagagca
     5401 cgtgcacggg gccacgcgcg agcgcctcga tcgaggcggc caggacggcg ctcgcgtcgg
     5461 tgacgtcgag gcggcgcgtt tcgcagccgg tgcgggcgcg cagatcgtcg agcagcgaat
     5521 cgtcgatgtc ggtggcgatg acccgcgcac cttcgcgtac gaacgcctcg gccgtggcgc
     5581 ggccgatgcc ctgtccggcg gcggtgatga aggcggtctt gccggcgagg cggtcggtca
     5641 tgaaaggctc cgtgaggctt gcagggtgag gcagtggggt gacgccgaac ccgaccgggc
     5701 ccggtaaaga atagtttcgc cgggattgcc cggtcaatgc cgctttcccg gcaagcctgc
     5761 tgcccacctc gcaacagcga ccgcggccgc ccgcgaaagg cggacacgca ccgctggcca
     5821 tccgtcgtgc cggcgcggcg ggaggcgttt cgtttgatag ccttcgcgac aaacaaaagc
     5881 gacgcgacgc gcgcgccgat catggaccag cctcccgatc tcctgccact cgccccgtcg
     5941 ccgcctccgt tgctgctgcc gccgcccgac aggccgcggc gcagccgttg gctgggcggc
     6001 atcgtcgccg tcctcgcact gctggcgctc ggcgccctgg cctggtacct gacgcatcgc
     6061 ccggcggctt cgcccggcgg tttcggcccc gccgccgcgg cgtcgggcgc gtcgggcgcc
     6121 gcggcccggc cggtcggcgc tccgggagga cggggcggac ggttcggcgc cgccagcacc
     6181 gtcggcatcg ccgcggcgaa gcgcgccgat ctgccggtct acatcgatgc gctcgggacg
     6241 gtcctcgccg cggtgacgac gacggtgcga ccgcaggtct cgggcgtgat cacccggatc
     6301 gcctacaccg aagggcagat ggtgaagaag ggccagctgc tggcgacgat cgatccgcgc
     6361 tcgttcgaga tcgcgctgca gcaggcgatc ggcgcgcgga tgcgcgacga ggccacgctg
     6421 caaagcaaca aggtgcagct gcagcgctac cagacgctgc tcagccagga ttcgatcgcc
     6481 cgccaggacg tcgacacgct cgccgcgacg gtcaagcagc tcgagggaac gatcgtcatc
     6541 gaccgggcca acgagagcac cgcacgcctg aacctcagct actgccgcat cgtcgcgccg
     6601 atcgacggcc gggtcggcct gcggccgatc gatcagggca actacatcgc cgccggcgac
     6661 accaacggcg tcgcggtcat cacccaggtc gagccgatca acgtcgagtt cgcggtccct
     6721 caggacctca tcccgcagat ccgttcgcgg gtcgcctcgg gcgcgaagct cgccgtgacc
     6781 gccttcgacc ggacccgtac cgtcaagctc gacgagggca cgttctcgac cctcgacaac
     6841 cagatcgaca cgcagaccgg cacggtgaag gccaaggcgc gcttcgccaa caccgcacgg
     6901 cagctctttc ccaaccagtt cgtcaacgtc cggctgctgc tgcaggtgga tcagggcgcg
     6961 atcgtcattc ctgtgacggc gatccgcacc gggccgaacg gcgacttcgt ctacgtgctc
     7021 aacgacgacc gcaccgtctc cgtcaggccg gtgactcgcg gcatcgccac gacgaatctc
     7081 gtcgccatcg acaagggcct cgaagccggc gagcaggtcg tcaccgaggg cggcgatcgc
     7141 ctgaaggacg gcgcccgggt ccagctggcc gccgacctgc cgccggcagg cgcctcggcg
     7201 gcggcccgcg cccatggtgc acggcgcggc gcctcggccg ccgaaggcgc cgcgtcgggc
     7261 catcgccgcc gctcgctcga tggcattccg gctgcggctt cggcgcctgc ctcttgagtc
     7321 cttcccgccc gttcatccag cggccggtgg cgacctcgct gctgatgctg gcgatcgtcc
     7381 tcgcgggact ggtcggcttc aagttcctgc ctctctcggc gctgccgcag gtcgactatc
     7441 cgaccatcca ggtgcagacc ttct
//
LOCUS       DASRWS010000063         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3196205, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000063 DASRWS010000000
VERSION     DASRWS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196205"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /gene="pnuC"
                     /locus_tag="VFF72_01140"
     CDS             <1..592
                     /gene="pnuC"
                     /locus_tag="VFF72_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HZV91786.1"
                     /translation="AFVVAGSVVTWLEIVAFVLALVMVVCNMRVDPRGWPLAIASSLL
                     YFLLFWKSRLYGDASLQIFFAVIACWGWWQWLRGTDASGQALRVRRLGRRGRWIALAA
                     LAVAWPATGLFLGRFTDTDVPWWDAFPTSASLIGQWLLGRKYIENWPTWLVVNVVGTA
                     LFAYKGLWLTVVLYAVFAVLSVAGWRAWRRLPASAA"
     gene            589..1143
                     /locus_tag="VFF72_01145"
     CDS             589..1143
                     /locus_tag="VFF72_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV91787.1"
                     /translation="MTRAWTVALVGAESTGKTTLARDIASALEARGQRVVVVDEALRE
                     FCALRGRTPHQDEQEGLAREQTRRIAEAAASEAIDVVVADTTALMIAVYSDFVFGDRK
                     LYIAAEAAQALIDLTLLTALDLPWQADGLQRDGEHVRVPVDALVRAALERAGVAVEPV
                     AGSGSDRLAAAMAAIDQARSRAKR"
     gene            complement(1190..1669)
                     /locus_tag="VFF72_01150"
     CDS             complement(1190..1669)
                     /locus_tag="VFF72_01150"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZV91788.1"
                     /translation="MLYALLKTLHLLGVIAWLGGMFFVLACLRPAAAEVLAPPERASL
                     MQATLGRFLSLVAWTAVLILITGATMASLAGRVAHRNGLAFNVPLDWYAMAGLFVVMI
                     AVLAHIRLVLFRALERAVAARAWADAGRAMGAMRWEVVINLVIGVFIVVVVRLGRAA"
     gene            1795..>2169
                     /locus_tag="VFF72_01155"
     CDS             1795..>2169
                     /locus_tag="VFF72_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZV91789.1"
                     /translation="MTKLCSVCHAENRDDAQFCRSCGTAFPPSAAPATSADDAQLAAG
                     IVCDECGFQNKPGVRYCANCGVSLLGTVIVPRSRSGLPGDPYAQPTSPPPISYPSFAP
                     VAPYPAPPATAPMYPPVGSTSFA"
BASE COUNT          323 a          766 c          750 g          330 t
ORIGIN      
        1 cgccttcgtc gtcgccggca gcgtcgtcac ctggctcgag atcgtcgcct tcgtgctggc
       61 gctggtgatg gtcgtctgca acatgcgcgt cgatccgcgc ggctggccgc tggcgatcgc
      121 cagctcgctc ctctacttcc tgctgttctg gaaaagccgc ctctacggcg atgccagcct
      181 gcagatcttc ttcgccgtga ttgcctgctg gggctggtgg cagtggctgc gcggcaccga
      241 tgcgtcgggg caggcgcttc gtgtgcgacg actgggccgg cgcggccgct ggatcgccct
      301 tgccgcgctg gcggtcgcct ggccggccac gggcctcttc ctgggtcgct tcaccgacac
      361 cgacgtgccg tggtgggacg cctttccgac atcggccagc ctcatcggcc agtggctgct
      421 cggtcgaaag tacatcgaga actggccgac gtggctggtg gtcaacgtcg tcggcacggc
      481 gctcttcgcc tacaagggcc tgtggctcac ggtcgtcctg tacgccgtct tcgcggtcct
      541 ctcggtggcg ggctggcgcg cctggcgcag gctgccggcg agcgctgcat gacccgagcc
      601 tggaccgtcg ccctcgtcgg tgccgaaagc accggcaaga cgacgctcgc gcgagacatc
      661 gcctcggccc tcgaagcgcg cggccagcgc gtcgtcgtcg tcgacgaggc gctgcgcgag
      721 ttctgcgcgc tgcgcggccg gacgccgcac caggacgaac aggaaggcct cgcccgcgaa
      781 cagacgaggc gcatcgccga ggccgccgcg agcgaggcga tcgacgtcgt cgtcgccgat
      841 acgacggcgc tgatgatcgc cgtctacagc gacttcgtct tcggcgaccg caagctctac
      901 atcgccgccg aagcggcaca ggcgctgatc gacctcacgc tcctcaccgc gctcgacctg
      961 ccctggcagg ccgacgggct gcagcgcgat ggcgagcacg tgcgcgtccc ggtcgatgcg
     1021 ctcgtgcgtg ctgcgctcga gcgggccggc gttgccgtcg aacccgtcgc cggcagcgga
     1081 agcgaccgcc tcgccgcggc catggcggcg atcgaccagg cgcgctcgcg cgcgaagcgc
     1141 tgagtcgctc gcgttgccga tcgctgacga tcgctcggcg acgatcggct caggcggccc
     1201 tgcccagcct gacgacgacg acgatgaaca cgccgatgac caggttgatc acgacttccc
     1261 agcgcatcgc gcccatcgcc cggccggcgt cggcccaggc ccgcgcagcg acggcgcgct
     1321 cgagggcccg gaacaggacg agacggatgt gcgcgagcac ggcgatcatg acgacgaaaa
     1381 gaccggccat cgcataccag tcgagcggca cgttgaaggc gagcccgttg cgatgggcca
     1441 cccgcccggc caggctcgcc atcgtcgcgc cggtgatcag gatgagcacg gcggtccaag
     1501 ccacgagcga cagaaatcgg ccgagggtcg cctgcatcag gctcgcgcgc tcgggcggcg
     1561 cgagcacctc agcagcagcc gggcgcaggc aggccagcac gaagaacatg ccgcccagcc
     1621 aggcgatgac gccgagaaga tgcaaggtct tgagcagggc atagagcatg tcggtctccg
     1681 tttgtcggcg tggcgcggtg gcattgtcgt tcgcaggacg ccgcgaattg ccccgaccca
     1741 caaaccgcgg tatcgtcgat cggcaatgag ggatggggcg tggaggccga cgaaatgacg
     1801 aagctgtgtt cggtgtgcca tgccgaaaac cgggacgacg cccagttctg ccgctcgtgc
     1861 ggcacggcat ttccaccttc ggcggcgccg gcgacgagtg ccgacgatgc gcagctcgcc
     1921 gccggaatcg tctgcgacga atgcggcttc cagaacaagc cgggcgttcg ctactgcgcg
     1981 aactgcggcg tcagtcttct cggaacggtg atcgttccgc gctcgcgcag cggcctgccg
     2041 ggcgatccct acgcgcagcc cacttcgccg ccgccgatca gctatccgtc gttcgcgccg
     2101 gtcgcaccct atccggcgcc gccggcgacg gcgccgatgt acccgcccgt cggatcgacg
     2161 agctttgca
//
LOCUS       DASRWS010000064         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_318734, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000064 DASRWS010000000
VERSION     DASRWS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318734"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFF72_01160"
     CDS             <1..1281
                     /locus_tag="VFF72_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZV91790.1"
                     /translation="HDSMLLNLSGAHAPFFTRNVVIVADSAGRTGIGEVPGGTAIEQV
                     VVEAGALLVGHAIGEMRDLLAGVERRFADRDGAGRGLQTFDQRTTVHAVTGLECALLD
                     LLGQHLGVPVSALLGEGRQRDSVPVLGYLFYIGDRRRSDRPYRAGAAAATDDWLALRD
                     EPALDPAAIVRLAEAAAARYGFADFKLKGGVLAGDAEVEAAVALHERLPEARITLDPN
                     GCWSLAEATRLLRDRRDVLAYAEDPCGAEGGFSGREVLAEFRRATAMPTATNMVATDW
                     RQLAHALALQAVDIPLADPHFWTMAGAVRVAQTCRDRGLTWGSHSNNHFDVSLALFTH
                     VAAAAPGHITAIDTHWIWQDGEALTTAPLRIEGGAIRVPDRPGLGVELDMAAVERAHA
                     LYLQHGLGGRDDAASMQALDSGWRFDPKRPCLVR"
     gene            1321..2109
                     /gene="garL"
                     /locus_tag="VFF72_01165"
     CDS             1321..2109
                     /gene="garL"
                     /locus_tag="VFF72_01165"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HZV91791.1"
                     /translation="MPTPSIPYRSFPNSFRQRLLGGETLIGCWCSLANATTAEVLGVA
                     GFDWLLLDGEHSSNDVVTFIPQLMALKDSVSAPIARPASNNPVEIKRLLDAGFYNFLV
                     PMVESPDEARRAVQATRYPPQGIRGVSVSQRSNRFGTVPDYFKGINEQICVLVQIESR
                     KGLEAAAEIAAVDGIDGVFIGPADLSAAFGHLGQPDHPESQAAIAAVFKAVDSVGKAI
                     GILAPVEADARRYLQMGARFVAVGSDLGVFRGATQALRDKFMPR"
     gene            complement(2142..2522)
                     /locus_tag="VFF72_01170"
     CDS             complement(2142..2522)
                     /locus_tag="VFF72_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV91792.1"
                     /translation="MSPSRLRTYVVEDSSIIRDNLTEALQESAPVDVVGSAEDEQAAV
                     DWLLDPKHQCDLVIVDIFLKSGSGLGVLKKIARLDDSVRRVVLSNHATPEVRAKCAEL
                     GAHKVFDKSNEIDEMLGYVNRLVH"
BASE COUNT          399 a          963 c          897 g          413 t
ORIGIN      
        1 cacgacagca tgctgctcaa cctgagcggt gcgcacgcgc cgttcttcac gcgcaatgtc
       61 gtcatcgtcg ccgactcggc gggccgcacc ggcatcggcg aggtgccggg cggaacggcg
      121 atcgagcagg tcgtcgtcga ggccggtgcg ctgctcgtcg gccatgcgat cggcgagatg
      181 cgcgacttgc tcgccggcgt cgagcgacgc ttcgccgatc gcgatggcgc gggccgcggc
      241 ctgcagacct tcgaccagcg caccaccgtc cacgccgtca ccggcctcga atgcgcgctc
      301 ctcgacctgc tcggacagca tctcggcgtg cccgtctcgg ccctgctcgg cgagggccgg
      361 caacgcgaca gcgtgccggt cctgggttac ctcttctaca tcggcgaccg gcggcgcagc
      421 gacaggccct atcgcgccgg ggcggccgct gcaaccgacg actggctggc gcttcgcgac
      481 gaaccggcgc tcgaccccgc cgcgatcgtc cgcctcgccg aggcggccgc cgcgcgctac
      541 ggctttgccg acttcaagct gaaaggcggc gtgctcgccg gcgacgcgga agtggaagcc
      601 gcggtcgccc tgcacgaacg gttgcccgaa gcgcgcatca ccctcgatcc gaacggctgc
      661 tggtcacttg ccgaggcgac gcgactgctg cgcgaccgcc gcgacgtgct cgcctacgcc
      721 gaagatccgt gcggcgccga aggcggcttc tcgggccgcg aagtgctcgc cgagtttcgt
      781 cgcgccacgg cgatgccgac agcgacgaac atggtcgcca ccgactggcg ccagctcgcc
      841 cacgcgctgg ccctgcaggc cgtcgacatc ccgctggccg acccgcactt ctggacgatg
      901 gccggcgcgg tccgcgtcgc ccagacgtgc cgcgaccggg gcctgacgtg gggctcgcac
      961 tcgaacaacc acttcgacgt ctcgctcgcc ctgttcacgc acgtcgccgc cgccgcgccc
     1021 gggcacatca cggcgatcga cacgcactgg atctggcagg acggggaggc gctcacgacg
     1081 gcgccgctgc gcatcgaagg cggcgccatc cgcgtgcccg accggccagg gctcggtgtc
     1141 gagctcgaca tggcggcggt cgagcgggcc cacgcgctct acctgcagca tggcctgggc
     1201 gggcgcgacg acgccgcgtc gatgcaggca ctcgattccg gctggcgctt cgacccgaag
     1261 cgaccctgtc tcgtgcgatg aattccggcg ccgcggcgcc gcaacagcga aagaacctcg
     1321 atgccgacgc cttccatccc ctatcgatcg ttcccgaaca gctttcgcca gcgcctgctc
     1381 ggcggcgaga cgctgatcgg ctgctggtgc tcgctcgcca acgcgacgac cgccgaggtc
     1441 ctgggcgtcg ccggcttcga ctggctcctt ctcgacggcg agcactcgtc gaacgacgtc
     1501 gtcaccttca ttccgcaact gatggcgctc aaggacagcg tcagcgcgcc gatcgcgagg
     1561 ccggcgtcga acaacccggt cgagatcaag cgccttctcg acgccggctt ctacaacttc
     1621 ctcgtcccga tggtcgaaag ccccgacgag gcgcgccgcg cggtccaggc gacgcgctat
     1681 ccgccgcaag gcattcgcgg cgtgtcggtc tcgcagcgca gcaaccgctt cggcaccgtc
     1741 cccgactact tcaagggcat caacgagcag atctgcgtcc tcgtgcagat cgagagccgg
     1801 aaggggctcg aggccgcggc ggagattgcc gcggtcgacg gcatcgacgg cgtcttcatc
     1861 ggcccggccg acctctcggc cgcattcggt catctgggcc agcccgacca tccggaatcg
     1921 caggccgcga ttgccgccgt cttcaaggcc gtcgattcgg tcggcaaggc gatcggcatc
     1981 ctggcgcccg tggaggccga tgcccgccgc tatctgcaga tgggcgcgcg cttcgtcgcc
     2041 gtcggcagcg acctgggcgt ctttcgcggt gccacccagg cgttgcgcga caagttcatg
     2101 ccacgctgat cacgcgtcgg ggagcgcgcc cgggcgccgg atcagtggac caggcggttg
     2161 acgtagccca gcatctcgtc gatctcgttg gacttgtcga agaccttgtg cgcgccgagc
     2221 tcggcgcact tggcgcgaac ctccggcgtt gcatggttgc tcagaaccac gcggcgcacc
     2281 gaatcgtcga gacgggcgat cttcttgagg acgccgaggc cactgcccga cttcaggaag
     2341 atgtcgacga tgacgaggtc gcattgatgc ttcggatcga gcagccaatc gacggcggcc
     2401 tgctcgtcct cggcgctgcc gacgacgtcg accggggcgc tttcctgaag cgcctcggtc
     2461 aggttgtcac gaatgatcga gctgtcttcg acgacatagg tgcgcaggcg gcttgggctc
     2521 atggcgaatt gaagcatcgt tgccaattct ggagacttcg cggggccaat ggggtaggcg
     2581 gcgcggcgag gcaacgctcg acaaccggct tccgtcgcgt cggacgcttc cgaccacgcg
     2641 gcacgaacct ggcgcgccga cgtggcacgt cg
//
LOCUS       DASRWS010000065         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1757049, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000065 DASRWS010000000
VERSION     DASRWS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757049"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1554)
                     /locus_tag="VFF72_01175"
     CDS             complement(16..1554)
                     /locus_tag="VFF72_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HZV91793.1"
                     /translation="MADPLPIARHGNVEIDLLPALANRHGLVTGATGTGKTVTLQVIA
                     ERMSAIGVPVFMADVKGDLAGMSQPGALSPKLGEHLKLIGAPAPAFAAMPVVFWDIFG
                     AQGHPVRATVSDLGPLLLARILGLNETQQGVLALAFKLADDNGLLLLDLKDLRALLQY
                     AADNAQGLQTSYGNISGASIGAIQRGLLALEQQGGGEFLGEPMLNIDDLLQTDGGRGA
                     VNILAADRLMRSPQLYATMLLWLLSELYERLPEVGDREKPKLVFFFDEAHLLFTDAPK
                     ALLDKIEQVVRLIRSKGVGVFFVTQNPLDVPDIVLGQLGNRVQHALRAFTPRDEKAVK
                     SAASTMRANPAIDTEKAITELGVGEALISFLDEKGRPNMVERAFVLPPASRIGAITPE
                     ERKAVMERSPVKGVYEKTVDRESAYEKLKGRAAAGADAAAEAKAEASTSKGDAAPTTA
                     AGGGILGGLSDVLFGSTGPRGGRHEGLAESAARSAVRSIGSSVGREIIRGVLGGIFGG
                     GRRR"
     gene            1693..2139
                     /gene="rimP"
                     /locus_tag="VFF72_01180"
     CDS             1693..2139
                     /gene="rimP"
                     /locus_tag="VFF72_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZV91794.1"
                     /translation="MGLGDLLERTVPPLGYELVDWDMSARTRLVRVFIDKPDGVDVED
                     CARVSNHLTRLFAVENVDFERLEVSSPGLDRPVKRLADYARFKGQEAQLTLSAPIDGA
                     RRVKGILRGTEGDDVLVETPAGVRSFPIGTIERTRLVPAIDWRKGS"
     gene            2136..>2512
                     /locus_tag="VFF72_01185"
     CDS             2136..>2512
                     /locus_tag="VFF72_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HZV91795.1"
                     /translation="MNRELLLLVDALSREKNVPKEIVFTALEAALASATKKKYADNID
                     ARVSIDRETGDYESFRRWTVVPDEEHEEPAHQIAITDAAERDPALALGDVVEAPLEPV
                     EFGRIGAQAAKQVILQKIRDAER"
BASE COUNT          403 a          867 c          845 g          397 t
ORIGIN      
        1 gggttcgcac cgtcctcagc gccgccgccc gccgccgaag atgccgccga gcacgccgcg
       61 gatgatctcg cgtccgacgc tcgagccgat gctgcgcacc gccgagcgcg ccgccgattc
      121 ggccaggcct tcgtgcctgc cgccgcgcgg gccggtcgag ccgaagagca cgtcggacag
      181 gccgccgagg attccgccgc cggcggccgt cgtcggggcc gcgtcgccct tcgacgtgga
      241 cgcttcggcc ttggcctcgg cggccgcatc ggcgcccgcc gccgcgcggc ccttcagctt
      301 ctcgtaggcc gactcgcgat cgaccgtctt ctcgtacacg cctttcaccg gcgaacgttc
      361 catcaccgcc ttgcgctcct cgggcgtgat tgcgccgatg cgcgacgcgg gcggcaggac
      421 gaacgcgcgc tccaccatgt tcggccggcc cttctcgtcg aggaacgaga tcagcgcctc
      481 acccacgccg agctcggtga tcgccttctc ggtgtcgatc gccggattgg cgcgcatcgt
      541 cgacgcggcc gatttcacgg ccttctcgtc gcgcggcgtg aaggcgcgaa gcgcgtgctg
      601 gacgcggttg ccgagctgcc ccagcacgat gtcgggcacg tcgagcgggt tctgcgtcac
      661 gaagaacacg cccacgccct tcgagcggat cagccgcacc acctgctcga tcttgtccag
      721 cagcgccttg ggcgcatcgg tgaacagcag gtgcgcctcg tcgaagaaga acacgagctt
      781 cggcttctcg cggtcgccca cttcgggcag tcgctcgtac agctccgaca gcagccacag
      841 cagcatcgtc gcgtagagct gcggcgagcg catcaggcga tcggcagcca ggatgttgac
      901 ggcgccgcgg ccaccgtcgg tctgcagcag atcgtcgata ttcagcatcg gctcaccgag
      961 aaactcgccg ccgccctgct gctcgagcgc cagcaatccg cgctggatcg cgccgatcga
     1021 cgcgccggag atgttgccgt acgacgtctg caggccttgc gcgttgtcag ccgcgtattg
     1081 cagcagcgcg cgcagatcct tcaggtcgag cagcagcagg ccgttgtcgt cggcgagctt
     1141 gaaggcgagc gccagcaccc cctgctgcgt ttcgttcagg ccgaggatgc gcgccagcag
     1201 cagcggcccg aggtcgctca ccgtggcgcg gaccggatgc ccctgcgctc cgaagatgtc
     1261 ccagaacacc accggcattg cggcaaacgc cggtgcgggc gctccgatca gcttcagatg
     1321 ctcgccaagc ttcggcgaca gcgcgccggg ctggctcatg ccggcgagat cgcccttgac
     1381 gtccgccatg aataccggca cgccgatcgc gctcatccgc tcggcgataa cctgcaacgt
     1441 tacggtcttg cccgtcccgg tcgcgccggt gacgagcccg tggcgattgg cgagcgccgg
     1501 gaggaggtcg atttcgacat tgccatgccg ggcgatcggc agcgggtcgg ccattggcgg
     1561 gactccagga aacgcgcgta gcgggagttt agcgcggatg gcaaaatacc cggtttcctg
     1621 ctagaatctg cccggataag taatgagaaa tgggctgggc gcagcccatt tttttttgga
     1681 aatcgccatc caatgggcct cggtgatctg ctggaacgga ccgtgccccc gctcgggtac
     1741 gaactcgtcg actgggacat gtcggcgagg acccggctcg tccgcgtgtt catcgacaag
     1801 ccggacggcg tcgacgtcga ggattgcgca cgggtgtcga accacctgac gcggcttttc
     1861 gccgtcgaga acgtcgactt cgagcgcctc gaggtttcgt cgccggggct ggatcgcccg
     1921 gtcaagcggc ttgcggacta cgcgcgcttc aagggtcagg aagcgcagct gacgctcagt
     1981 gcgccgatcg acggcgcgcg gcgagtcaag ggaattctgc gcggcaccga gggcgacgac
     2041 gtcctcgtcg agacgcctgc cggcgtgcga tcgtttccga tcggcacgat cgagcgcacg
     2101 cggctcgtgc ccgccatcga ctggagaaaa ggatcatgaa ccgcgaactg ctgctgctcg
     2161 tggacgcgct gtcgcgcgag aagaacgtgc cgaaggagat cgtcttcacg gcgctcgagg
     2221 ccgcgctcgc gtcggccacg aagaagaagt acgccgacaa catcgacgcg cgcgtttcca
     2281 tcgaccgcga gacgggcgac tacgagtctt tccgccgctg gaccgtcgtg cctgacgaag
     2341 agcacgagga gccggcgcac cagatcgcga tcaccgacgc ggcggagcgc gatcccgcgc
     2401 tcgcgctcgg cgacgtcgtc gaggcgccgc tcgagccggt cgagttcggc cgcatcggcg
     2461 cgcaagccgc gaagcaggtg atcctgcaga agatccgcga cgccgagcgc ga
//
LOCUS       DASRWS010000066         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2043711, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000066 DASRWS010000000
VERSION     DASRWS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043711"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFF72_01190"
     CDS             complement(<1..1003)
                     /locus_tag="VFF72_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZV91796.1"
                     /translation="MLHRLAIRDFVIVDALEVEFGSGLTVLTGETGAGKSILVDALQL
                     ALGNRGDAGTVREGAARAEVSAEFDAPAAIAGWLDEGGFEPGADADAGRLLLRRSIDA
                     QGKSRAWINGRAATVAQLRDVADRLVDIHGQHAWQSLTRPAAVRGVLDAYARLDTRPL
                     GMAWAVWRDAMQRLAAAEARQGDLERERERLAWQIGEVERLAPGADEWPELDAEHTRL
                     ANAQSLLDAGRAALEAIGGGESESTGNAEASTGRALALLEEVGRFDARLQATIEALRG
                     AEAQLHEASHALASYLDRAELDPQRLQQLDERISSWLALARRYRRPPGELAALLGEWQ
                     "
     gene            complement(1055..1930)
                     /locus_tag="VFF72_01195"
     CDS             complement(1055..1930)
                     /locus_tag="VFF72_01195"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZV91797.1"
                     /translation="MSTRFRHAALVGKYQSGGIREELESIGRFLESRGLRVTLEAETA
                     RQTGIAAWPEMQADELGCECDLAVVVGGDGTMLGIARQLARHGTPLVGINQGRLGFIT
                     DVSLVGFASALGPMLEGEYDAEERTMLEGNVRRDDATIFESFALNDVIVSRGATAGMV
                     ELRIEIDGEFVANLRADGLIVGSPTGSTAYALSAGGPILHPGIAGWVVVPIAPHALSN
                     RPIVVPDDGEVQVEIVAGRDASVSFDMQSLSSLGHGDRICVRRSPHRVRFLHPRGWSY
                     FATLRGKLHWASGEA"
     gene            1999..>2270
                     /locus_tag="VFF72_01200"
     CDS             1999..>2270
                     /locus_tag="VFF72_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZV91798.1"
                     /translation="MLDERAKTLLKTLVERYIEDGQPVGSRTLSRASGLELSPATIRN
                     VMADLEDLGLIASPHTSAGRVPTARGYRLFVDAMLTAQPQRLPSWPR"
BASE COUNT          316 a          827 c          810 g          317 t
ORIGIN      
        1 cctgccattc gccgagcagg gcagcgagct cgcccggcgg ccggcgatag cgccgcgcca
       61 gcgccagcca ggacgagatt cgctcgtcga gctgctgcag tcgctgcggg tcgagctcgg
      121 cccggtcgag atagctggcg agggcgtgcg acgcttcgtg caactgcgcc tcggcgccgc
      181 gcagcgcctc gatcgttgcc tgcagccgcg cgtcgaagcg gccgacctcc tccagcagcg
      241 cgagggcccg gccggtcgac gcctcggcgt tcccggtcga ctcggactcg cctccgccga
      301 tcgcctcgag agcggcgcgg ccggcgtcca gcagcgactg ggcgttggcg agtcgggtgt
      361 gctcggcatc gagctccggc cactcgtcgg cgcccggagc gagtcgttcc acctcgccga
      421 tctgccaggc gagtcgttcc cgctcgcgtt cgagatcgcc ttgccgggct tcggccgcgg
      481 caagccgctg catcgcgtcg cgccagaccg cccaggccat gccgagcggc cgggtgtcga
      541 ggcgggcata ggcgtcgagc acgccgcgca ccgcggccgg ccgcgtcagg ctctgccagg
      601 catgctggcc gtggatgtcg acgagacgat cggccacgtc gcgcaactgg gcgacggtgg
      661 ctgcgcggcc gttgatccag gcccggctct tgccttgcgc atcgatcgat cgacgcagca
      721 gcagccgccc ggcgtcggca tcggcgcccg gctcgaagcc gccttcgtcg agccagccgg
      781 cgatcgccgc gggggcgtcg aattcggcgc tgacctcggc ccgcgccgcg ccttcgcgaa
      841 ccgtgccggc gtcgccgcgg ttgccgaggg ccagctgcaa ggcatcgacg aggatcgact
      901 tgccggcacc tgtctcgccc gtgagcaccg tcaggccgga cccgaactcg acctcgagcg
      961 cgtcgacgat cacgaagtcg cggatcgcga gccggtgcag cacgtgcgcg tcccgcctac
     1021 gggcctgcgt gcgcgaatgc gcgccggtca gccgtcatgc ttcccccgac gcccagtgga
     1081 gcttgccgcg cagtgtcgcg aagtagctcc agccccgcgg atgcaggaag cggacgcggt
     1141 gcggcgagcg gcgcacgcag atgcggtcgc cgtggccgag gctgctcagc gactgcatgt
     1201 cgaagctgac gctggcgtcg cggccggcga cgatctcgac ctggacctcg ccgtcgtcgg
     1261 gaacgacgat cggccggttc gagagcgcgt gcggcgcgat cggcacgacg acccatccgg
     1321 cgatgcccgg atgcaggatc ggaccgcccg ccgacagcgc gtaggccgtg gagccggtgg
     1381 gcgagccgac gatgaggccg tcggcgcgca ggttggcgac gaactcgccg tcgatctcga
     1441 tgcgcagctc gaccatgccg gcggtcgcgc cgcggctgac gatgacgtcg ttgagggcga
     1501 agctctcgaa gatcgtcgca tcgtcgcggc gcacgttgcc ttcgagcatg gtccgctcct
     1561 cggcgtcgta ctctccttcg agcatcgggc cgagcgccga ggcgaagccg acgagcgaga
     1621 cgtcggtgat gaagccgagc cgcccctggt tgattcccac cagcggcgtg ccgtgacgcg
     1681 ccagctggcg ggcgatgccg agcatggtgc cgtcgccgcc gacgacgacg gcgaggtcgc
     1741 attcgcagcc gagctcgtcg gcctgcatct cgggccaggc ggcgatgccg gtctggcgcg
     1801 ccgtctcggc ctcgagcgtg acgcgcaggc cgcgcgactc gaggaatcgg ccgatcgact
     1861 cgagttcctc gcgaatgccg cccgactggt acttgccgac gagcgcagcg tgccggaagc
     1921 gggtcgacat tcgcgggatt acaccacagc gacccttgtt cgcgcgggcg aaaatggctt
     1981 gcagcacact gcgccaccat gctcgacgag cgcgccaaga cactcctcaa gaccctggtc
     2041 gaacgctaca tcgaagacgg ccagccggtc ggctcgcgca cgctctcgcg tgcgagcggc
     2101 ctcgagctgt cgccggcgac gatcaggaac gtcatggccg atctcgagga tctcggcctg
     2161 atcgccagcc cgcacaccag cgccggccgc gtgcccaccg cgcgcggcta ccgcctcttc
     2221 gtcgacgcca tgctcacggc gcagccgcag cggctgccct catggccgcg
//
LOCUS       DASRWS010000067         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3767342, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000067 DASRWS010000000
VERSION     DASRWS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767342"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFF72_01205"
     CDS             complement(<1..851)
                     /locus_tag="VFF72_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZV91799.1"
                     /translation="MADRVFQPETLAIHAGQIPDAATGARALPIYQTTSFVFDSADHA
                     ASLFNLATFGNVYSRLSNPTVAALEERVAALEQGRAGLASASGMAAEAMVLMTLLGQG
                     DHVVAAGALYGGSVSMLAVNLERLGITTTFVDAASPDAFAAAMRPRTRAVFAESIGNP
                     SLAILDIAAVADVAHAHGVPLVVDNTVPSPFLCNPIRFGADIVVHSATKYLGGHGTTL
                     GGVIVESGRFPWDNGKFPGMTEPSRGYHGVRFYETFGDFGFTMRARMETLRVYGATLS
                     PTSAWQIL"
     gene            complement(915..1382)
                     /locus_tag="VFF72_01210"
     CDS             complement(915..1382)
                     /locus_tag="VFF72_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZV91800.1"
                     /translation="MTDDIATLRRILATSRTIAVVGLSAEWHRPSHFAAAYMQQHGYR
                     IVPVNPRYEEVLGERCHASLETIEVPVDIVDVFRRTEDVMPIARQAIAIGAKCLWQQI
                     GVKNVEAARLAKAAGLDAVMDRCVKIEHARLFGGLHWAGVNTGVVSARRPIAT"
     gene            complement(1465..3735)
                     /gene="mdoH"
                     /locus_tag="VFF72_01215"
     CDS             complement(1465..3735)
                     /gene="mdoH"
                     /locus_tag="VFF72_01215"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HZV91801.1"
                     /translation="MNGRPVAHPPVRRSAMRPVPWAGPLLRRPWRRLRGRLAPVAVDH
                     HESSWPMARRVAFFCLVLFGAWLGTEAMVEVLPAHGEGAVEIALLVLFAVLFGWISAG
                     FWTGLMGVFVLLRGPGHGPLAHGLAEAWLRPLDPRARTAIIMPICNEDVPTVFGGLEA
                     TIESLYATGESANFDVYVLSDSSDPDIRAAEQAAWSDLASRRAAVLPDGVAGLRVHYR
                     WRQRRSKRKAGNVADFCRRWGSGYRYFVVLDADSVMSGECLTSLVRLMEAHPDAGIIQ
                     TAPRPVGHETLHARIQQFCGRAYGPLFTAGMCFWQLGESQYWGHNAILRTAPFIEHCA
                     LAPIEGNGSLSGEILSHDFVEAALMRRAGWKVWVADELDGSYEQVPPNLLAELQRDRR
                     WCQGNLQNSRLMFEPAIHAVHRSGFLTGVLAYASSPLWLVFLVLSTLLFARHAGHDPV
                     YFFAAGQLFPSWPTANLKLMLTLFGLTAILLLAPKALSLLAIVLRGDAGRFGGTSRLL
                     ASAAIEFLHSLLLAPVRMLFHSQFVIAAITGWKLDWKSPPRDDAATGWAEAWRRHGVH
                     TWLTLAWICAIVAGSDAMAWWLTPILLGLLGAAPLSVWGSRTRPGKALRRRDLLLTPE
                     ELACPAVLARAMGRGEEVARRLVSFRAAMVSDVDARRCLAGNAPAGVPRGMKGLAESL
                     RMERASREGPGTLSDDERMRLLSSSLAAHRLREDVLARRAHPAWSAPRPSSSRAVPAP
                     AELGDALGALLPGAGD"
     gene            complement(3732..>4682)
                     /locus_tag="VFF72_01220"
     CDS             complement(3732..>4682)
                     /locus_tag="VFF72_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HZV91802.1"
                     /translation="EFWIERPGPRATSLVIYGLLDSKRVTGAYRFVLTPGTETVMAVS
                     ARLYLRQPIAKLGLAPLTSMYAFGENQPGHDDYRPEVHDSDGLSIESADGEWIWRPLV
                     NPKRLLVTSFALTDPRGFGLMQRDRSYSSYEDPEAQYERRPSAWVEPTGSWGSGRVEL
                     VQIPTPDETNDNVVAYWVPKLAPQPGRPFELSYRIHWQSQGTLPSRKGWVVQTRRGRG
                     FTQRRDGDINFVVDFDGPPLRNLPPGEGPEPVIWADANADVHERNLFRNPVTGTWRMT
                     VRFHRLDSAKPVELRAYLKGPHSTLTETWSYVVPPEPEKS"
BASE COUNT          778 a         1589 c         1649 g          666 t
ORIGIN      
        1 aggatctgcc aggcgctggt cggcgacagg gtcgcgccat aaacgcgcag cgtttccatg
       61 cgggcccgca tcgtgaagcc gaaatcgccg aaggtttcgt agaagcgcac gccgtgatag
      121 ccgcgcgacg gctcggtcat gccgggaaac ttgccgttgt cccaggggaa gcggccgctt
      181 tccacgatca cgccgccgag cgtcgtgccg tggccgccga ggtatttggt cgccgagtgg
      241 acgacgatgt cggcaccgaa gcggatcggg ttgcacagga acggcgacgg caccgtgttg
      301 tcgacgacca gcggcacgcc atgcgcgtgc gccacgtcag cgacggccgc gatgtcgagg
      361 atggcgaggc tcgggttgcc gatgctctcg gcgaacaccg cgcgggtccg gggtcgcatg
      421 gccgcggcga aggcgtcggg cgaggccgcg tcgacgaagg tcgtggtgat gccgagacgc
      481 tcgaggttga ccgccagcat gctgacggag ccgccataca gcgcgccggc ggcgacgacg
      541 tgatcgccct ggccgagcag cgtcatcagc accatcgcct cggccgccat gccgctcgcc
      601 gaggcgagcc cggctcgccc ctgttcgagc gccgcgacgc gttcctcgag cgcggcgacc
      661 gtcgggttcg agaggcgcga atagacgttg ccgaaggtcg cgaggttgaa caggctggcg
      721 gcgtgatcgg cgctgtcgaa gacgaagctg gtcgtctggt agatcggcag cgcccgggca
      781 ccggtcgcgg cgtccggaat ctggccggcg tggatcgcca gcgtctcggg ctggaagact
      841 cgatcggcca tctcagtgtc gggcgggccg gcgcggtcgc gcccctgccg cgggtgtcag
      901 gtgctcgacg cgcttcatgt cgcgatcggc cgccgcgcag agacgacgcc cgtgttcacc
      961 ccagcccaat gcaggcctcc gaaaaggcgc gcatgctcga tcttgacgca acgatccatg
     1021 accgcatcga ggcccgcggc cttcgccagc cgcgccgcct cgacgttctt cacgccgatc
     1081 tgctgccaca ggcacttcgc accgatcgcg atggcctgcc gggcgatcgg catgacgtct
     1141 tcggtgcggc gaaagacatc gacgatgtcg accggcacct cgatcgtctc gaggctggcg
     1201 tgacaacgtt cgccgagaac ctcttcgtag cgcggattca ccggcacgat ccgatagccg
     1261 tgctgttgca tgtaggcggc cgcgaaatga ctcggccgat gccactcggc cgaaaggccg
     1321 acgacggcga tcgtccggct cgtggcgagg attcggcgaa gcgtggcgat gtcgtcggtc
     1381 atggcggcgc gggccgcgtg ctggcgcgcc cgtctcggac cttacccgaa acgcagcacc
     1441 gccgcgaccc ggcggcgacc ggtctcagtc gccggcgccg ggcaggagcg cgccgagcgc
     1501 gtcgccaagt tcggcaggcg ccgggacggc ccgcgacgac gacggacgcg gtgccgacca
     1561 cgccggatgg gcgcgccggg ccaggacgtc ctcacgcagc cggtgagctg ccagcgagct
     1621 cgacagaagc cgcatccgct cgtcatcgct cagcgtgccg gggccttcgc gcgacgcgcg
     1681 ctccatccga agcgactcgg cgagaccttt catccccctc ggcacgcccg cgggcgcatt
     1741 tccggcaagg cagcgtcggg cgtcgacgtc gctcaccatc gcggcgcgaa aggagacgag
     1801 gcgccgggcc acttcctcgc cgcggcccat cgcgcgggcg aggacggctg gacacgccag
     1861 ttcctcgggt gtcagcagca ggtcccgccg gcgcagcgcc ttgccagggc gggtacggct
     1921 gccccagacg gaaagcggcg ccgcgccgag caggccgagc aggatcggcg tcaaccacca
     1981 agccatcgcg tcgctgccgg cgacgatcgc gcagatccac gccagggtca gccacgtgtg
     2041 cacgccgtgc cggcgccacg cctcggccca accggtggcc gcgtcgtcgc gcggcggtga
     2101 tttccagtcg agcttccagc ccgtgatcgc ggcaatcacg aactgtgaat ggaagagcat
     2161 gcgcaccggc gccagcagca acgaatggag gaattcgatc gccgcgctgg cgaggagccg
     2221 gctggtgccg ccgaaccggc cggcgtcacc gcgcagcacg atcgccagca gcgatagtgc
     2281 cttcggcgcc agcagcagga tcgcggtcag tccgaacagg gtcagcatga gcttcaggtt
     2341 ggcggtcggc cagctcggga acagttgtcc ggcggcgaag aaatagaccg gatcgtgccc
     2401 ggcgtgccgg gcgaagagca gcgtcgacag gacgagaaat accagccaga gcggcgacga
     2461 ggcgtaggcg aggacgccgg tgagaaagcc gcttcgatgc acggcgtgga tggccggctc
     2521 gaacatcaga cgggaattct gcagattgcc ctggcaccag cggcggtcgc gctgcagttc
     2581 ggcgagcagg ttgggcggca cctgctcgta gctgccatcg agctcgtcgg cgacccagac
     2641 cttccagccg gcccttcgca tcaacgcggc ctcgacgaag tcgtgcgaca ggatctcgcc
     2701 cgagagcgac ccgttgccct cgatcggcgc gagtgcgcaa tgctcgatga aaggtgccgt
     2761 tcgcaggatc gcgttgtgtc cccagtattg cgattcgccg agctgccaga agcacattcc
     2821 ggcggtgaac agcggcccgt acgcgcggcc gcagaattgc tggatgcggg cgtgcagcgt
     2881 ctcgtgtccg accggccgtg gcgccgtctg gatgattccg gcgtcgggat gcgcttccat
     2941 caggcgcacc agcgaggtca gacattcgcc gctcatgaca ctgtcggcat cgaggacgac
     3001 gaaataccgg tagccgctgc cccagcggcg acagaagtcg gcgacgttgc cggccttgcg
     3061 cttgctgcgc ctctgccgcc accggtagtg gacccgcagg cccgccacgc cgtcgggcag
     3121 caccgcggca cgccgcgaag cgagatcgct ccacgccgcc tgctcggcgg cccggatgtc
     3181 gggatcgctg ctgtcgctga ggacgtagac gtcgaagttg gccgactcgc cggtcgcgta
     3241 cagcgattcg atcgtcgcct cgaggccgcc gaacaccgtc ggcacgtcct cgttgcagat
     3301 gggcatgatg atcgccgtgc gcgcccgcgg gtcgagcggg cgcagccagg cctccgcgag
     3361 accgtgtgcc agcggtccgt gtccagggcc gcgaaggagc acgaacacgc ccatgagacc
     3421 ggtccagaag ccggccgaaa tccatccgaa gaggacggcg aagaggacca gcaaggcgat
     3481 ctcgaccgcg ccctcgccgt gtgctggaag aacctcgacc atcgcctcgg tgccgagcca
     3541 ggcaccgaac agcacgagac agaagaacgc gacgcggcgc gccatcggcc aggagctctc
     3601 gtggtggtcg accgccaccg gcgccagcct gccgcgcagg cgccgccagg gccgacgcag
     3661 cagcggcccg gcccagggca cggggcgcat ggccgagcgc cgcacgggcg ggtgggcgac
     3721 agggcgaccg ttcatgactt ttcgggctcc ggcggcacga cgtagctcca tgtttcggtc
     3781 agcgtcgagt gcgggccctt gagataggcg cgcaattcga ccggcttggc cgagtcgagt
     3841 cgatgaaagc ggaccgtcat tcgccacgtg ccagtgaccg gatttcgaaa caggtttctc
     3901 tcgtgcacat cggcattggc gtcggcccag atcaccggct cggggccctc gcctggcggg
     3961 aggttgcgca gcggcggccc gtcgaaatcg acgacgaaat tgatgtcgcc gtcgcgccgt
     4021 tgcgtgaagc cgcggccacg ccgcgtctgc accacccatc ccttgcgcga aggcagcgtg
     4081 ccctgcgact gccagtgaat ccggtacgaa agctcgaacg gtcggcccgg ctgaggggcg
     4141 agcttcggaa cccagtaggc gacgacattg tcgttggtct cgtcgggggt cgggatctgc
     4201 accagctcca cgcggccgct gccccaggag cccgtcggct cgacccacgc gctcggacgc
     4261 cgctcgtact gcgcctccgg atcctcgtag ctcgaatacg agcggtcgcg ctgcatgagg
     4321 ccgaagccgc gaggatcggt cagcgcgaaa gaagtgacca gaagccgctt cggattgacc
     4381 aggggccgcc agatccattc gccgtcggcc gactcgatcg acaggccgtc ggaatcatgc
     4441 acttcgggcc ggtagtcgtc atggcccggc tggttctcgc cgaatgcata catgctggtg
     4501 agcggcgcca ggcccagctt cgcgatcggc tggcgcaggt aaaggcgagc cgacaccgcc
     4561 atcaccgttt ccgtgcccgg ggtcagaacg aaccgatagg cgcctgtcac gcgtttcgag
     4621 tcgagcaggc cgtagatgac gagcgacgtc gcgcgcggtc cgggacgctc gatccagaac
     4681 tc
//
LOCUS       DASRWS010000068         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1180693, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000068 DASRWS010000000
VERSION     DASRWS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180693"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VFF72_01225"
     CDS             complement(<1..1451)
                     /locus_tag="VFF72_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZV91803.1"
                     /translation="MAENTIQWDKDADGIVTLTLDDPTGSANVMNESYIESMGKAVDR
                     LVAEKDSITGVVITSGKKTFFAGGDVKSMIKIGPENAGEAFDLVETVKKQLRTIETLG
                     KPVVAALNGAALGGGLEIALACHHRIAADVKGSQFGFPEATLGLLPGAGGVTRTVRML
                     GIQNAFVTVLSQGTRFKPAKAKELGLIDELLPTVEELVPAAKAWIKANPDSHEQPWDK
                     KGYKMPGGTPSSPGLASILPSFPALLRKQIKGAPMPAPKAILAAAVEGAQVDFDTASR
                     IESRYFTSLVTGQVAKNMIQAFFFDLQAINGGGSRPDGIEPVKINKIGVLGAGMMGAG
                     IAYVSAKAGYDVVLKDVSLEAAQKGKGYSEKLEAKALERGRTTQERSDALLARITPTA
                     DPADFKGVDFVIEAVFESQELKHKVFQEIEDIVEPNALLGSNTSTLPITGLATGVKRQ
                     EDFIGIHFFSPVDKMPLVEIIKGEKTSDEALARV"
     gene            complement(1456..2667)
                     /locus_tag="VFF72_01230"
     CDS             complement(1456..2667)
                     /locus_tag="VFF72_01230"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZV91804.1"
                     /translation="MPEEAFIYEAIRTPRGKQRNGALTEVKPLSLVVGLIEELRKRNP
                     DLDENLISDVILGCVSPVGDQGGDIARAAVLASGMPVTSGGVQLNRFCASGLEAVNTA
                     AQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIE
                     GFTRDDVDNYALRSQEKAAEAWSGGYFAKSVVPVRDQNDLLILDHDEHMRPDTTKEGL
                     GKLKPAFEGLAAMGGFDDVALQKYHWVEKINHVHTGGNSSGIVDGAALVMIGSEAAGK
                     SQGLTPRARIVATATSGADPVIMLTGPTPATKKVLDRAGLTVDDIDLFELNEAFASVV
                     LKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERRNARRALVTLCIGG
                     GMGVATIIERV"
BASE COUNT          422 a          903 c          884 g          514 t
ORIGIN      
        1 acgcgggcca gcgcctcgtc agaagtcttc tcgcccttga tgatttcgac cagcggcatc
       61 ttgtcgaccg gcgagaagaa gtggatcccg atgaagtcct cctgccgctt gaccccggtc
      121 gccagaccgg tgatcggcag cgtggaggtg ttggagccga gcagcgcgtt gggctcgacg
      181 atgtcctcga tctcctggaa caccttgtgc ttgagttcct ggctctcgaa caccgcctcg
      241 atcacgaagt cgacgccctt gaagtcagcc gggtcagcgg tcggagtgat gcgcgccagc
      301 agggcgtcgc tgcgctcctg cgtggtgcga ccgcgctcca gcgccttggc ctccagcttc
      361 tcggagtagc ccttgccctt ctgtgcggcc tccaacgaaa cgtccttgag gacaacgtca
      421 tagccagcct tggcggagac gtacgcgata cccgcgccca tcatgccggc cccgagcaca
      481 ccgatcttgt tgatcttgac cggctcgatg ccgtcgggcc gcgatccacc tccgttgatg
      541 gcctgcaggt cgaagaagaa cgcctgaatc atgttcttgg cgacctggcc ggtgaccagc
      601 gaggtgaagt agcggctctc gatgcggctg gcggtgtcga agtcgacctg cgcaccctcg
      661 accgcggcgg ccaggatggc cttcggcgcc ggcatcggcg cacccttgat ctgcttgcgc
      721 agcaacgccg ggaacgacgg caggatcgac gccagccctg gcgacgacgg ggtgccgccg
      781 ggcatcttgt agcccttctt gtcccacggc tgctcgtgcg agtccgggtt ggccttgatc
      841 caggccttgg ccgcgggcac cagctcctcg accgtcggca gcaactcgtc gatcagaccg
      901 agttccttgg ccttggccgg cttgaagcgg gtgccctgcg acagcacggt gacaaatgcg
      961 ttctggatac ccagcattcg cacggtgcgc gtgacaccgc cggcacccgg cagcaggccc
     1021 agcgtcgcct cggggaagcc gaactgcgac cccttcacgt cggccgcgat gcggtgatgg
     1081 cacgccagcg cgatctccag gccgccgcca agcgcggcgc cgttgagcgc ggccacgacc
     1141 ggcttgccca gggtctcgat cgtgcgtagc tgcttcttta cggtttcgac cagatcgaag
     1201 gcctccccgg cgttctcggg accgatcttg atcatgctct tgacgtcacc gccggcgaag
     1261 aaggtcttct tcccgctggt gatcaccaca ccggtgatcg aatccttctc cgcaacaagg
     1321 cgatccacgg ccttgcccat cgactcgatg tagctttcat tcatcacgtt ggccgaaccc
     1381 gtcgggtcgt ccaaggtcag cgtgacgatg ccgtcggcat ccttgtccca ctgaattgtg
     1441 ttttcggcca tgtacttaaa ccctctcgat gatcgtcgcg acacccatgc cgccgccaat
     1501 acaaagcgtg acgagcgcac gccgggcgtt gcgacgctcg agttcgtcga ccatggtgcc
     1561 cagaatcatt gcgccggtgg ctcccagcgg gtgacccatg gcgatggcgc cgccgttgac
     1621 gttgagcttc tcgtcgggaa tgttgaggtc cttctggaac ttcagcacca cggaggcgaa
     1681 ggcctcgttc agctcgaaca ggtcgatgtc gtcgacggtc agaccggcgc ggtcgagcac
     1741 cttcttggtc gcgggggtcg ggccggtcag catgatcacc gggtcggcgc cgctggtcgc
     1801 ggtggcgacg atgcgcgccc gcggggtcag gccctgcgat ttgccggcgg cctcggagcc
     1861 gatcatgacc agcgcggcgc cgtcgacgat cccggaggag ttaccgccgg tgtgcacgtg
     1921 gttgatcttc tccacccagt ggtacttctg cagcgccacg tcgtcgaagc cgcccatcgc
     1981 ggccagaccc tcgaaggccg gcttcagctt gcccaggccc tccttggtgg tgtccggccg
     2041 catgtgctcg tcgtggtcca ggatcagcag gtcgttctgg tcgcgaaccg gcaccacgga
     2101 cttggcgaag tagccgcccg accacgcctc ggcggccttc tcctggctgc gcagtgcgta
     2161 gttgtccacg tcgtcgcggg tgaagccctc gatggtggcg atcaggtcgg cgccgatgcc
     2221 ctgcgggacg aacatgacgt cgtagttggt ggccgggtcc aggcccatgg cgccaccgtc
     2281 ggatcccatc ggcacccggc tcatcgactc cacgccaccg gccagcacca ggtcatccca
     2341 gccggaacgg accttctgcg cggcggtgtt gacggcctcg aggcccgagg cacagaagcg
     2401 gttgagctgg acgccgccgg aggtgaccgg catgccggag gccagcaccg cggcgcgggc
     2461 gatgtcgccg ccctggtcgc cgacgggtga gacgcagccc aggatgacgt cgctgatcag
     2521 gttctcgtcg aggtcggggt tgcgcttgcg cagctcctcg atcaggccga cgaccaggct
     2581 caatggcttg acttcggtca gcgcaccgtt gcgttgcttg ccgcgcggcg tgcggatggc
     2641 ctcgtagatg aaggcttctt cgggcatgtc gacttcctgt tcgagaaaaa gggtgtgtta
     2701 gatccgtgtt aaagactagg tct
//
LOCUS       DASRWS010000069         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2331666, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000069 DASRWS010000000
VERSION     DASRWS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331666"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFF72_01235"
     CDS             complement(<1..945)
                     /locus_tag="VFF72_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZV91805.1"
                     /translation="MMIAGIDVGGTFTDLVVVDPASGEVRVAKVPTTADNQAFGVLAV
                     LDAAGVDPAALEAIVHGTTTTTNAMLERKIATVGLITTRGFRDVLELGRRTRPTPYGL
                     TGRFVPLIDRRLRLEIDERLDAEGRVLVPLDEAQVEAAARELVRLGAESVVIHFLHSY
                     INPAHEARAAEIVRGVWPNGYVTAGHAIVAEYREYERGTTAAVNAAIQPVLHRYIERL
                     RSELGRRGFARDLMVMQGNGGTVSSTIVAEHAVSTVMSGPASGVIAAAATAMQAGDSP
                     GEFADIVTYDMGGTSSDVALVRGGQPSVSSDLELEYAMP"
     gene            complement(968..2584)
                     /locus_tag="VFF72_01240"
     CDS             complement(968..2584)
                     /locus_tag="VFF72_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV91806.1"
                     /translation="MSEAEAMQANRSAPPAAPLLSVRGLTKSFGGVSAVQGVNFDVAA
                     GEMLALIGPNGAGKTTCFNMVSGQLTPDAGSVKLGGAELIGRRPDQIWRLGVGRTFQI
                     AETFASLTLLENVQLALLSNAGRVGRVWRPARTQFRDEAAALLESVGMAAQAGRAASV
                     LAYGDVKRVELAIALAHRPRLLLMDEPTAGMAPRERNELMALARRLASEQSIGVLFTE
                     HSMDVVFACADRIVVLARGELIAEGPPAQVRDDAKVREVYFGGGTTFEAAAAESPASV
                     AVPPEDAMVRTAGPMPPAAGLPTATRTADVLEVGALDAWYGRAQVLYGVDLAVAEGEC
                     VALVGRNGAGKSTTMKAIMGLLGRKRGRVVFRGADISALASYRIGRLGLGWVPEDRRI
                     FTDLSVAENLEVARQPPRTGAPAWTPERVYALFPNLAAMTDRPGGRMSGGEQQMLTVA
                     RTLMGNPYLVLLDEPSEGVAPLIVEEIARMIAALKREGVAILLCEQNLHFAGLVADRA
                     VLLEKGEVKFAGPMARLMADDGLLRTHLGV"
     gene            complement(2581..>3420)
                     /locus_tag="VFF72_01245"
     CDS             complement(2581..>3420)
                     /locus_tag="VFF72_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV91807.1"
                     /translation="LMIDMLIAVLFASSLYFLMGPAGLHSFGHAAYFGLGAYGAALFL
                     KALGWPMEVSLLLAPLLAAAGALVFGWFCVRLSGVYLAMLTLAFAQIVWSVVFQWDDL
                     TGGSNGLVGIWPSAWLAPKPVYYLLVLAFTVAGCGLVVRMLHAPFGLALRGSRDSPLR
                     AAAIGLRVPRLQWAAFTIAGTLAGLAGSLFAFAKGTISPETLGVGKSVDGLVMVLLGG
                     VQAVSGPWVGGALFTWLQDTVARQTDYWRALLGIVMLALVLVFPLGVAGGLKRLFIRD
                     AAA"
BASE COUNT          533 a         1251 c         1195 g          441 t
ORIGIN      
        1 cggcatcgcg tattcgagct cgagatcgct cgacaccgac ggctggccgc cacgcacgag
       61 cgcgacgtcg ctcgacgtgc cgcccatgtc gtaggtgacg atgtcggcga actcgccggg
      121 cgagtcgccc gcctgcatcg ccgtcgccgc ggcggcgatg acgcccgacg ccgggcccga
      181 catcaccgtc gacaccgcgt gctcggcgac gatcgtcgac gagaccgtgc cgccgttgcc
      241 ctgcatcacc atgaggtcgc gggcaaagcc gcgccggccg agctcgctgc gcagccgctc
      301 gatgtagcgg tgcagcaccg gctggatcgc ggcgttgacg gccgccgtcg tgccgcgctc
      361 gtattcgcgg tattcggcga cgatggcgtg gccggcagtg acgtatccgt tcggccagac
      421 gccgcggacg atctcggcgg cccgcgcctc gtgcgccgga ttgatgtagc tgtgcaggaa
      481 gtggatgacg acgctctcgg cgccgaggcg gacgagctcg cgcgccgccg cctcgacctg
      541 ggcctcgtcg agcggcacca ggacgcggcc ttcggcgtcg agccgctcgt cgatctcgag
      601 gcgaagacgc cggtcgatga gcggcacgaa gcggccggtc aggccgtacg gcgtcggccg
      661 cgttcttcgg ccgagctcga ggacgtcgcg aaagccgcgc gtcgtgatga ggccgaccgt
      721 cgcgatcttg cgctcgagca tggcgttggt cgtcgtcgtc gtgccatgga cgatcgcttc
      781 cagcgcggcc ggatcgacgc ctgccgcgtc gagcaccgcc agcacgccga aggcctggtt
      841 gtcggccgtc gtcggcacct tcgcgacgcg cacctcgccg ctcgccggat cgacgacgac
      901 gaggtcggtg aaggtgccgc cgacatcgat tcccgcgatc atcatgagcc gcctttcctc
      961 acacgcctca cacgcccagg tgcgtgcgca gcaggccgtc gtcggccatc agccgcgcca
     1021 tcgggccggc gaacttcacc tcgcccttct cgagcagcac ggcgcgatcg gcgacgaggc
     1081 ccgcgaaatg caggttctgc tcgcagagca ggatggcgac accctcgcgc ttcagcgcgg
     1141 cgatcatcct ggcaatctcc tcgacgatca gcggcgcgac gccttccgaa ggctcgtcga
     1201 gcagcaccag gtaggggttg cccatcagcg tgcgcgccac ggtgagcatc tgctgctcgc
     1261 cgccgctcat gcggccgccc ggtcgatcgg tcatcgcggc gaggttcgga aacagcgcgt
     1321 agacgcgctc cggcgtccac gccggtgcgc cggtacgcgg cggctggcgc gcgacctcga
     1381 ggttttcggc aacgctcagg tcggtgaaga tgcgccggtc ttccggcacc cagccgagac
     1441 cgagacggcc gatgcgatac gaagccagcg ccgagatgtc ggcgccgcga aagacgaccc
     1501 ggccgcgctt tcggccaagc aggcccatga tcgccttcat cgtcgtcgac tttccggcgc
     1561 cgttgcggcc gaccagcgcc acgcactcgc cttcggccac cgccaggtcg acgccgtaga
     1621 ggacctgcgc ccgcccgtac caggcgtcga gcgcgccgac ctcgagcacg tcggctgtcc
     1681 tcgtcgccgt cggcagcccg gccgccggcg gcatgggtcc ggcggtgcgc accatcgcgt
     1741 cttcaggtgg aacggccacg ctggcaggcg actctgccgc cgcggcctcg aaagtcgtgc
     1801 cgccgccgaa gtagacctcg cgcaccttcg catcgtcgcg cacttgcgcg ggcggccctt
     1861 cggcgatcaa ctcgccgcgc gccagcacga cgatgcggtc ggcgcaggcg aagacgacgt
     1921 ccatgctgtg ctcggtgaag agcacgccga tcgactgctc actcgccagg cgacgggcca
     1981 gcgccatcag ctcgttgcgt tcgcgcggcg ccatgccggc cgtgggctcg tccatcagca
     2041 gcaggcgcgg ccggtgtgcg agcgcgatcg ccagctcgac ccgcttgaca tcgccatagg
     2101 cgagcacgct ggcggcgcgc ccggcctgcg cggccatgcc gacgctctcg agaagggccg
     2161 ccgcctcgtc gcgaaactgc gtccgcgccg gccgccacac gcggcccacg cggccggcgt
     2221 tcgaaagcag cgccagctgc acgttctcga gcaacgtcag cgaggcgaaa gtctcggcga
     2281 tctggaaggt gcggccgacg cccaggcgcc agatctggtc gggccgccgg ccgatcagct
     2341 ccgcgccgcc gagcttcacc gaccccgcat cgggcgtgag ctgcccgctg accatgttga
     2401 agcaggtcgt cttgccggcg ccgttcggcc cgatcagcgc cagcatctcg ccggcagcga
     2461 cgtcgaagtt cacgccctgc acggcgctga cgccgccgaa cgacttggtg agcccgcgca
     2521 cggagagcag cggcgccgcc gggggcgcgc tgcggttcgc ctgcatcgcc tcggcttcgc
     2581 tcatgccgcc gcgtcgcgga tgaagagcct cttcaggccg ccggcgacgc cgagcgggaa
     2641 gacgagcacg agcgccagca tgacgatgcc gaggagcgcg cgccagtagt cggtctgccg
     2701 cgccaccgtg tcctgcagcc aggtgaagag cgcgccgccg acccacgggc ccgacaccgc
     2761 ctggacgccg ccgagcagca ccatgaccag gccgtcgacc gacttgccga cgcccagcgt
     2821 ctccggcgag atcgtgccct tggcgaacgc gaagagcgag ccggcgagcc ccgcgagcgt
     2881 gccggcgatg gtgaaggcgg cccactgcag gcgcggtacg cggaggccga tcgccgccgc
     2941 gcgcagcggc gagtcgcggc tgccgcgcag cgccagcccg aacggcgcat gcagcatgcg
     3001 aacgacgagg ccgcagccgg cgacggtgaa ggccagcacc agcaggtagt acaccggctt
     3061 gggcgcgagc caggccgagg gccagatgcc gaccagtccg ttgctgccgc cggtgaggtc
     3121 gtcccactgg aagacgaccg accagacgat ctgcgcgaag gccagcgtca gcatcgccag
     3181 gtagacgccc gacaggcgga cgcagaacca gccgaagacg agcgcgccgg ccgcggcgag
     3241 aagcggcgcc agcagcagcg agacctccat cggccagccg agcgccttca ggaacagcgc
     3301 ggccccgtag gcgccgaggc cgaaatacgc agcgtggccg aacgagtgca gacccgccgg
     3361 tcccatgagg aaatagaggc tggaggcgaa cagcacggcg atcagcatgt cgatcatcag
//
LOCUS       DASRWS010000070         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2909496, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000070 DASRWS010000000
VERSION     DASRWS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909496"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFF72_01250"
     CDS             complement(<1..261)
                     /locus_tag="VFF72_01250"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZV91808.1"
                     /translation="MNKLDNDTPRERHKSDPKLANAWKSKSGRDLSDAEVLAMPDSEY
                     MNEKQIEFFRMKLAALKDDLLSNAGETTEHLREDTSIVPDPAD"
     gene            446..889
                     /locus_tag="VFF72_01255"
     CDS             446..889
                     /locus_tag="VFF72_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91809.1"
                     /translation="MAAIVEGGGVAGAPFPGGLAAGAGAPFADGARPAAFSRAGAAGV
                     DLRAAGLATPCFDASLAAVDEGFFVAFDGFAAGALFLAAAGFAALLAATGLAAAAFFL
                     AGATVVFDDVFLPADVLTVPPRSGGTAPHGKWGGRSNAVFGRRGL"
     gene            complement(1001..2068)
                     /locus_tag="VFF72_01260"
     CDS             complement(1001..2068)
                     /locus_tag="VFF72_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZV91810.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLSEAHGQKIAVIENEFGDEN
                     IDNEILVQDSKEQIIQLNNGCVCCSIREDLRTTLADLAEKKRKGELDFDRVVIETTGL
                     ADPGPVAQTFFMDDEIAESYLLDSILTLVDAKHGDQTLDDRQEARRQVGFADQIFISK
                     SDLVDDQTVEALIHRVKHMNPRAKQTKVDFGNVPIADVFDLKGFNLNAKLEIDPEFLK
                     ADEHDHGHSHGDHHDHAHDEGHDHEHGEHCDHPHHHAHDDDVKSFVFRADKAIDPGKL
                     EDFLGAIVQVYGPKLLRYKGVLNMKGSDRKVIFQGVHQLMGSDLGPKWAPGEKKTSKM
                     VFIGIDLPREILEQGLKQCVV"
     gene            2259..3152
                     /locus_tag="VFF72_01265"
     CDS             2259..3152
                     /locus_tag="VFF72_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZV91811.1"
                     /translation="MSEPSVARGSRGADKAAASSDRRNLFQKLVDLVSPGPESRDQLM
                     ESLAEAENKELIEPESRMMVEGVLRMADMTAGDVMVPTRHMDQLDIDAPFDEMLRVII
                     RTGHSRFPVFEGRRENVIGILMAKDLLKLQRSPDLNLRTLLRPAVFVPESKGLNDLLR
                     DFRSNRNHLAIVIDEFGATAGLLTIEDVLEEIVGEIEDEFDEKDGESSIYTLADGSQR
                     VAGDTPVSAVNEFFDVDLPTDEFDTIGGLVADALGRVPRRGESAKLSGLVFTVMLARG
                     GAVRWFKATRGAEEALGSDGA"
     gene            3304..>4067
                     /locus_tag="VFF72_01270"
     CDS             3304..>4067
                     /locus_tag="VFF72_01270"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZV91812.1"
                     /translation="MTRLRATAEVVFVALLGALQTLVYVHTAFWPAQMLAIGLLAWRV
                     GLASPGRAACLGLAYGTAWLVAGTWWLYVSMHRYGGLPAWMAALAVLGLSILLSLYLA
                     AAMAAVARWRPRSPLAAACVFAAAWLLAELARGVIFTGFPWLASGYAHVDSPFGGFAP
                     WLGVYGLGATVAGIAAAFGFSRLGELRAWPAPVAALVVAIGIGMLAGRVDFTRPTGTL
                     RVSLLQGNVPQDEKFEARYVPEALALTASQLDEAKG"
BASE COUNT          532 a         1338 c         1433 g          764 t
ORIGIN      
        1 gtcggccggg tcgggcacga tcgaggtgtc ttcgcgcagg tgctcggtcg tctcgccggc
       61 attgctcagg agatcgtcct tcagcgccgc cagcttcatc cggaagaact cgatctgctt
      121 ctcgttcatg tattccgagt cgggcatggc gaggacttcg gcgtcgctca ggtcgcggcc
      181 actcttggac ttccaggcgt tggccagctt cggatcggat ttgtgacgct ctcggggcgt
      241 gtcgttgtcc agcttgttca tctctcgggt cggtggccgg ggcggggcga atcgatcggc
      301 aaatcgggat gtcggagggg gcaggggcgc ctggaccgga gcaacgaaat acttcggcgt
      361 tccccgtcca ggcttgcgcg gaacgacgcc agcgggcacg gcaaacggcg cgcccgcagc
      421 gagcgtcggc accggcacgg cgaccttggc agcgatcgtc gaaggcggcg gcgtcgccgg
      481 cgcgcccttc ccgggcggcc tcgccgccgg agccggcgcg cccttcgccg acggggcgcg
      541 accagccgcc ttctcgcggg ccggcgcggc gggcgtggat ttgcgtgcag ccggcttggc
      601 gacgccatgc ttcgatgcaa gcttggcggc ggtcgatgaa ggtttcttcg tggccttcga
      661 cggcttcgcc gccggcgcac tcttcttggc cgcggctggc ttcgccgccc tcttggcggc
      721 gacgggcttg gcagcggccg ccttcttctt ggcgggtgcc acggtggtct tcgacgatgt
      781 tttcttgccg gctgatgtgc tcacggttcc tcctcgcagc ggcggcacag ccccgcatgg
      841 caagtgggga gggcgaagca atgccgtttt tggccgccgc ggattgtagt cacgcccttc
      901 gcgggccggc gcaccgtttt cgaggcggcg ccccgacggg tcccgcgagg ggccggaaac
      961 gcccgatccg ggctcgatca gccgcggtca cgggcgcaat tcagacgacg cactgcttca
     1021 ggccctgctc gagaatttcg cgcggcagat cgatgccgat gaagaccatc ttgctcgtct
     1081 ttttctcgcc cggcgcccac ttcgggccga ggtcgctgcc catgagctgg tgaacgccct
     1141 ggaagatcac cttgcgatcc gaccctttca tgttcagcac gcccttgtag cggagcagct
     1201 tgggcccgta gacctggacg atggcgccca ggaaatcctc gagcttgccc gggtcgatgg
     1261 ccttgtcggc gcgaaagacg aacgacttga cgtcgtcgtc gtgggcgtga tggtgcggat
     1321 ggtcgcagtg ctcgccgtgc tcgtggtcgt ggccctcgtc gtgcgcatgg tcgtgatggt
     1381 cgccatgcga gtggccgtga tcgtgctcgt ccgccttcag gaactcgggg tcgatctcga
     1441 gcttggcgtt gaggttgaat cccttcaggt cgaagacgtc ggcgatcggc acgttgccga
     1501 aatcgacctt cgtctgcttg gcccgcgggt tcatgtgctt gacgcggtgg atcagcgctt
     1561 cgaccgtttg gtcgtcgacg aggtcggact tgctgatgaa gatctggtcg gcgaagccca
     1621 cctggcggcg cgcctcctgg cgatcatcga gcgtctggtc gccgtgcttg gcgtcgacca
     1681 gcgtcaggat cgagtcgagc aggtagctct cggcgatctc gtcgtccatg aagaaggtct
     1741 gcgccaccgg cccggggtcg gccaggccgg tcgtctcgat gacgacgcgg tcgaagtcga
     1801 gctcgccctt tctcttcttc tcggcgaggt cggcgagggt ggtgcgaagg tcttcccgga
     1861 tcgagcagca gacgcagccg ttgttgagct ggatgatctg ctccttgctg tcctggacga
     1921 ggatctcgtt gtcgatgttc tcgtcgccga actcgttctc gatgacggcg atcttctggc
     1981 cgtgcgcctc gctcaggacg cgcttgagaa gcgtcgtctt gcccgagccg agaaagccgg
     2041 tgaggatggt ggcggggatc agactcatgg gcgtggacct tggttgttcg tggggcgttg
     2101 tcgcgcgacg cgcggcgtgg gtctatctgg gggcggcggc ggtttccgcc aaggtagcag
     2161 gcctgtcaag accgcaccgc ggcgggtcat tgcgttaggg taagctttcc gcacccaggc
     2221 ttcggcggac cgccagtttt accgcgcccc gcccatcgat gtcagaacct tccgtggccc
     2281 gcggctcgcg cggcgcagac aaggccgccg cctcgtcgga ccggcgcaat ctcttccaga
     2341 agctggtcga cctcgtctcg ccgggaccgg agtcgcgcga ccagctcatg gaatcgcttg
     2401 ccgaggccga gaacaaggag ctgatcgagc ccgagtcgcg gatgatggtc gagggcgtgc
     2461 tgcgcatggc cgacatgact gccggcgacg tcatggtgcc gacgcgacac atggaccagc
     2521 tcgacatcga cgcgccgttc gacgagatgc tgcgcgtcat catccgcacc ggccactcgc
     2581 ggtttccggt gttcgagggc cggcgggaga acgtcatcgg catcctgatg gccaaggatt
     2641 tgctcaagct gcagcgatcg cccgacctca atctgcgcac cctgctgcgg ccggcggtgt
     2701 tcgtgcccga atcgaaaggc ctcaacgacc tgctgcgcga cttccgctcc aaccgcaacc
     2761 acctggccat cgtcatcgac gagttcggcg ccacggcggg gctcttgacc atcgaggacg
     2821 tgctggagga gatcgtcggc gagatcgagg acgagttcga cgagaaggac ggcgagagca
     2881 gcatctacac cctggccgac ggcagccagc gggtcgccgg cgacacgccg gtctcggccg
     2941 tcaacgagtt cttcgacgtt gatctgccga ccgacgagtt cgacaccatc ggcggcctcg
     3001 tcgccgacgc tctcggccgg gtcccgcgac gcggcgaatc ggccaagctg tccggcctcg
     3061 tgttcaccgt gatgctggcg cgcggaggcg ccgttcgctg gttcaaggcg acgcgcggcg
     3121 ccgaagaggc gctcggctcg gacggcgcct gattgcgccc gcgagccggt ccggcgtggg
     3181 cgcccgaaga ttctttcgaa gccgcggcgt ggccagtcgc tggcgaacgc gcgccaggca
     3241 cccgtcgcga cgctcgccga tcggcgccat gacaaccgcc cgatcgatcc gccaggcgag
     3301 cgcgtgacga ggcttcgtgc cacggccgag gtcgtcttcg tggcgctgct cggcgcgctg
     3361 cagacgctcg tctatgtcca caccgccttc tggccggcgc agatgctggc gatcggcctg
     3421 ctcgcctggc gggtcggcct tgcgtccccg ggtcgtgcgg cgtgcctggg cctggcctac
     3481 ggcacggcat ggctggtcgc cggcacgtgg tggctctacg tcagcatgca tcggtacggc
     3541 ggtttgccgg cctggatggc ggcgcttgcc gtgctcggcc tgtcgattct gctgtcgctc
     3601 tatctggccg ctgcgatggc ggcggtcgcc cgctggcggc cgcgatcgcc gctggccgcc
     3661 gcgtgcgtgt tcgcggcggc gtggctgctg gccgagctgg cgcgtggtgt catcttcacc
     3721 ggctttccgt ggctggccag cggctacgcg cacgtcgatt cgccgttcgg cggtttcgcg
     3781 ccctggctcg gtgtctacgg gctgggtgcg accgtcgccg gcatcgccgc ggcgttcgga
     3841 ttcagccgcc tcggcgagct ccgcgcctgg ccggcgccgg tcgcggcgct cgtcgtcgcc
     3901 atcggcatcg gcatgctcgc cggccgcgtc gacttcaccc ggccgaccgg cacgctgcgg
     3961 gtatctctcc tgcaaggcaa cgtgccgcag gacgaaaagt tcgaggcccg ctacgtgccc
     4021 gaggccctgg ccctcaccgc ttcgcagctc gacgaggcca agggcga
//
LOCUS       DASRWS010000071         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3200543, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000071 DASRWS010000000
VERSION     DASRWS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200543"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..691)
                     /locus_tag="VFF72_01275"
     CDS             complement(245..691)
                     /locus_tag="VFF72_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZV91813.1"
                     /translation="MAHADTDDAPRSLCPISLALDVVGDKWSLLILRDLMFAGKNHYR
                     ELLQSDEGISSNILAERLKRLVEAGLLTKTDDPSHKQKAIYRLTAMGIDLLPAIAQLG
                     IWGRKYQPVTKESAAAAAALEKGGPELLKTMKVELRRQHLGKQAAT"
     gene            819..1409
                     /locus_tag="VFF72_01280"
     CDS             819..1409
                     /locus_tag="VFF72_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZV91814.1"
                     /translation="MKRQLIVQMQMSVDAVVSADDPELNWQLWNWGEAWPWDDALKAR
                     FNQTLAGVDTILLSRAMADEGYIDHWTRAGARFDDDPHYAFARRVAEAHKRVLTRQPL
                     DLRWPRTSQGHGPLFDEVAALKQAPGGDLIAFGGVGFTSRLLAAGVVDELQLYVNPTA
                     VVAGRRLFGQPGQRLQLIEAAQHACGIVVNRYIPLT"
     gene            complement(1934..3025)
                     /locus_tag="VFF72_01285"
     CDS             complement(1934..3025)
                     /locus_tag="VFF72_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZV91815.1"
                     /translation="MMRGRPKAELVVTEDERAQLTSFARSRSLPASLSARARIVLSSA
                     EGVANSSIAERLELGKATVGKWRARFIERRMAGLYDDVRPGPPRTIDDERVASLIKTT
                     LHTKPANGSTHWSVRSVSAETGISKSSVQRYFQLFGLQPHRSESFKLSNDPFFVEKLR
                     DVVGLYLSPPENALVLCVDEKSQCQALERTQPMLPMGFGYVEGVTHDYKRHGTTTLFA
                     ALNVLNGAVLATCKPRHRHQEFVSFLREIDKAVPTELDVHCIVDNYATHSHPKVKAWL
                     ASRPRWHMHFIPTYSSWLNQVERFFALITDKAIRRGSFTSVKQLVQRIDQFVTHHNIN
                     SQPFRWTATADSILEKLHRLCSRISGTGH"
BASE COUNT          588 a          982 c         1010 g          598 t
ORIGIN      
        1 aggagacggc gtagtccctg agccacagca cgccgggaac gaagatttcg agtgaaggtg
       61 caaccacgct accctgttgc agagggggtg caatccggat tgcctcgcga tcgaagcaag
      121 ggcgatcgcc gaagcatgtc acttgccgcg gctgactgcg atcgggctgc gcgaaaagca
      181 ccgcacacct gatcgagcac tcgccaacgc tcgtcagcca cgagcactga gcggctcggc
      241 gccttcacgt cgcggcctgc ttgcccaagt gctgccgccg cagctcgacc ttcatcgtct
      301 tcagtagctc aggccctccc ttctccagcg ccgcggccgc ggccgcgctc tccttcgtca
      361 ccggctggta cttgcggccc cagatgccga gctgcgcgat ggcgggaagc aggtcaatac
      421 ccatggccgt gaggcggtag atcgcctttt gcttgtggct cgggtcgtcg gtcttggtca
      481 gcagcccagc ctcgacaagg cgtttgagcc gctcggccag gatgttcgac gagatgcctt
      541 cgtcggactg cagcagctcg cggtagtggt tcttgcccgc gaacatcagg tcgcgaagga
      601 tcagcaggct ccacttgtcg cctaccacgt ccagcgccaa gctgatcggg cagagggagc
      661 ggggcgcgtc gtcggtatcg gcatgggcca tggcggatct tggaaatggt cgagactgct
      721 tgcatttttg cagcggtatg tctaaccttc aaccactttc actttgcaat cggtgttgcc
      781 ataaactcgc ccgaccgcaa gaccgaagga gaagcgcgat gaaacgacaa ctgatcgtgc
      841 agatgcagat gtccgtggat gccgtggtgt ccgcagacga tcccgagctg aattggcaac
      901 tgtggaactg gggcgaggct tggccgtggg acgatgcact caaggcccgc ttcaaccaga
      961 cgctggccgg cgtggacacc atcttactga gccgcgcgat ggccgacgaa ggctacatcg
     1021 accactggac ccgcgccggc gcacgtttcg acgatgaccc gcactacgcc ttcgcccgcc
     1081 gcgtggcgga agcgcacaaa cgcgtgctga cccgccagcc gctggacctg cgctggcccc
     1141 gtaccagcca ggggcacgga ccgttgttcg acgaggttgc ggcactcaag caagcgcccg
     1201 gcggcgacct gatcgccttc ggcggcgtcg gattcacctc gcggctgctt gccgcaggcg
     1261 tggtggacga actgcagttg tacgtaaacc caaccgcggt ggtcgctggc cgtcgcttgt
     1321 tcggccaacc cggccagcgc ctgcagctca tcgaggcggc gcaacatgcc tgcggcatcg
     1381 tcgtgaaccg ctacatcccg ctgacctgaa cctcgcgcgg cggcggccag agcggcgacg
     1441 gcggccgggc cgggtggtac aacagaatgg cgctggacgg gatcgccgga cgctttacaa
     1501 gtcagggcac ctcgagcaca tgcacttcat tgacgtccgg ctaccgagga gccagagggc
     1561 ggtctaacct cgccgaaggg ggctgtcctg cggccagccg ctcacgccga accttgaacg
     1621 tctgctttcg gaccgtcaac cgcgaccccg cctatttccg ctgggtggcg aaaacggaag
     1681 ttcggaagtt gcggggagaa gtctgcagcc gctgcagcgc cgtcatcggt tacgcccctg
     1741 gaagaagaat agcagcatcg cgaacagtcg cccgtgagca gagcgacggg gtaccccgtc
     1801 cacttgcatt cgtcatatca cgggacgtga tgctcgattg aagtcgccat gatgcttcgg
     1861 ccgtgtggtt agagagctcg caccatgctg gcctgcgaca ggacaaaaca tgagtgcaat
     1921 ctttcctttc gagctagtgt cctgtcccgc tgatacgtga gcaaagtcga tgcagttttt
     1981 cgaggatcga atcggccgtg gcagtccagc gaaacggttg tgagttgatg ttgtggtgag
     2041 tgacgaactg gtcaatgcgc tgcacgagtt gtttgacgct ggtgaacgag ccgcgacgga
     2101 ttgctttgtc ggtgatcagc gcgaagaagc gttcgacctg attgagccag gagctgtagg
     2161 ttgggatgaa atgcatgtgc caccgcggcc gacttgccag ccaagccttg accttcggat
     2221 ggctatgcgt ggcgtagttg tcgacgatgc aatggacgtc cagttcagtg ggcacagcct
     2281 tgtcgatttc acgcaggaac gacacgaact cttgatgccg gtggcgcggc ttgcatgtcg
     2341 ccagcaccgc gccgttgagc acgttcaacg ccgcgaacag ggtggtcgtg ccgtggcgct
     2401 tgtagtcgtg ggtcacgccc tcgacgtagc cgaaacccat gggcagcatc ggctgagtgc
     2461 gctccaacgc ttggcattgg ctcttctcgt ccacacagag aaccagcgcg ttctcaggcg
     2521 gactcaggta caggcccacc acgtcgcgca gcttctcgac gaagaacgga tcgttggaca
     2581 gcttgaagct ctccgagcga tgcggctgca atccaaagag ctggaagtag cgctgcacgc
     2641 tgctcttgga gatgccggtc tcggccgaca cggagcgcac gctccagtgg gtcgagccgt
     2701 tggccggctt ggtgtgcagc gtcgtcttga tcagactggc cacgcgctcg tcgtcgatcg
     2761 tgcgcggtgg cccgggtcga acatcgtcgt agagcccggc catgcggcgc tcgataaaac
     2821 gagcgcgcca cttgcccacc gtggccttgc ccagttcgag gcgctcggcg atcgaactgt
     2881 tggcaacgcc ctcggcgctg ctcaaaacga tgcgagctcg cgcactcaac gacgctggca
     2941 acgatcgact gcgcgcgaac gatgtgagct gcgctcgctc gtcttccgtc accaccaact
     3001 ccgccttcgg cctgcctctc atcatcgcga tatctccgtc ccaaagatac cgcagaggca
     3061 cgcactattt atgcctgcta tttgcgggac aggacactag ggattcgctg attaagcgcc
     3121 tgcggtgggc ggcttgttga agtgatgtcg ctccggcggg ccgagtcgaa gcaacttt
//
LOCUS       DASRWS010000072         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3200661, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000072 DASRWS010000000
VERSION     DASRWS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200661"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>2457
                     /locus_tag="VFF72_01290"
     CDS             31..>2457
                     /locus_tag="VFF72_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZV91816.1"
                     /translation="MHGDDVPDFLVSSDAWFRPTNIKLGPDGALYVADFYNKIIGHYE
                     VDLHNPGRDKQRGRIWRIVYKGTDAAPAPTKKFDLTRASVQDMVDDLASPNMTIRMLA
                     MNRLADIVGQPAIAPLKKVMAESSNIWQKIHGMWVLYRLGALDPAELKAAATDPDARL
                     REHAMRVLGEKSPWDSADDQLVLNGTRDPDPLVRRTAAAALGEHPSFEHVRPLLDLAE
                     RPDGDVFIEHVVRIALRDQMLVPGVCPKLQTARLSDADENLLAASAIGAPTSESASFL
                     LNYVTHASAAGEQITKQLKMIAKLVPAQQVDELAKFVQTRFSDDVELQLALFNAVQEG
                     LAERGTTTGEAMRAWGTSLATKLLEEGADNKDAWTFVPTTGKSDGQNPWGVQTRKSVD
                     GDNASAFLSSNVHSEQLTGTIRSHEFVVPPKLSFFLAGHNGQPPKKHKIKNFVTIRDA
                     KTGDVLATVNPPRNDVARQMTLDLRRFAGRRAYVEATDGDNQKAYAWMALGRFDPPVV
                     RVPVGGSMLENAVKIVQSLRLTPLAGKVEHLLTDGTQEPATRAAAARALGALDAPDHV
                     QALSSVVRDPRAADDVRQSAASVLATLDVPAASDAMVQAMQTAPQKLQLALAQALASG
                     RGGAEALLVAIEQGKASARLLQDSNVRERIGAVQPKDYEQRIEKLTRGLPAADAQIQA
                     MIDQRAAAFNPATASAKRGRLVFEKNCLICHAVANQGAHVGPPLDGIGIRGVPRLCED
                     ILDPSRNVAADFRQSTFVLSDGSVVAGIPRRTEGQTIVVADSTGKEVSIEKSKITRQA
                     ESKLSLMPSNF"
BASE COUNT          465 a          814 c          795 g          383 t
ORIGIN      
        1 gacgccaagc tcacgcctcg cggctccggg gtgcatggcg acgacgtgcc cgacttcctg
       61 gtcagcagcg atgcctggtt ccggccgacc aacatcaagc ttggccccga tggcgcgctg
      121 tacgtggccg acttctacaa caagatcatc gggcactacg aggtcgacct gcataatccc
      181 ggccgtgaca agcagcgcgg ccgaatctgg cggatcgtct acaagggcac ggacgccgcg
      241 cccgcgccga cgaagaagtt tgacctgacc agggccagcg tgcaggacat ggttgatgac
      301 ctggccagtc cgaacatgac catccgcatg ctggcgatga accgcctggc cgacattgtc
      361 ggccagcccg ctatcgcgcc gctcaagaag gtgatggccg agtcgtcgaa tatctggcag
      421 aagatccacg ggatgtgggt gctctatcgg ctcggcgcgc tcgacccggc ggaactcaaa
      481 gccgccgcga ccgatcccga cgcccggctc cgcgagcacg ccatgcgcgt gctgggcgag
      541 aagtcgccgt gggattcggc ggatgatcaa ttggtgttga acggaacccg cgatcccgat
      601 ccgctggttc gtcgcacggc tgctgccgct ttgggcgagc atccttcgtt cgagcacgtt
      661 cgcccgctgc tcgatctggc cgagcgcccg gacggggacg tgttcatcga gcacgttgtc
      721 cgcatcgcgc tccgcgatca gatgctcgtg ccgggcgtct gcccgaaact ccagaccgcg
      781 cgactcagtg acgcagacga gaacctgctg gccgcgtcgg ccatcggcgc gccgaccagc
      841 gaatcggcat catttctttt gaattatgtg acacacgcaa gcgccgcggg cgagcagatc
      901 accaagcagc tcaagatgat tgccaagctc gttcccgctc aacaggtcga cgaactggcg
      961 aagttcgttc agaccaggtt ctcggacgat gtcgaattgc agttggcgct gttcaacgcg
     1021 gtgcaggaag ggctcgccga gcggggaaca actaccggcg aggcgatgcg cgcatggggg
     1081 acctcgctgg cgacaaaact gctggaggaa ggcgccgaca acaaggacgc ctggacgttc
     1141 gtcccgacga cgggcaagtc cgacgggcaa aatccgtggg gcgttcagac ccgcaagtcc
     1201 gtcgatggcg acaacgcctc agcgttcctc tccagcaacg tccacagcga gcaactgacc
     1261 ggcacgatcc gctcgcacga gttcgtcgtg ccgcccaagc tctcgttctt tctggcgggc
     1321 cacaatggcc agccgccgaa gaaacacaag atcaagaatt tcgtcacgat tcgcgatgcg
     1381 aagaccggcg acgtgctggc gacggtgaac ccgccccgga atgacgtggc gcgacagatg
     1441 acgctggact tgcgccgatt cgccggccgg cgtgcgtacg tggaagccac cgacggcgac
     1501 aatcagaagg cttacgcctg gatggcgctg ggccgcttcg acccgccagt ggtgcgcgtc
     1561 ccggtcggcg gctcaatgct ggaaaatgcg gtcaagatcg tccaatcgct gcgactgacg
     1621 ccgctggcgg gcaaggtcga gcacctgctg accgacggca cccaggagcc ggccacgcgc
     1681 gccgcggcgg caagggcgct tggcgcgctg gacgcgcccg atcacgtgca ggccctctca
     1741 agtgtggtgc gcgacccaag ggccgctgac gacgtgcggc aaagcgcagc cagcgtgctg
     1801 gccaccctcg acgttcccgc cgcctcggat gcgatggtcc aggcgatgca gaccgcgccg
     1861 cagaagctgc aattggcact ggctcaggca ttggcctccg gtcggggtgg tgcggaagca
     1921 ctgctcgtcg ccatcgagca gggcaaggca tcggcacggc tgcttcagga ttccaacgtc
     1981 cgcgagcgga tcggcgccgt gcagccgaag gattacgagc agcggatcga gaagctgacc
     2041 aggggccttc ccgccgccga tgcgcagatc caggcgatga ttgatcagcg cgccgccgcg
     2101 ttcaacccgg cgactgcctc ggccaagcgc ggccgtctgg tgttcgagaa gaattgtctg
     2161 atctgccatg cggtcgctaa ccagggcgcc cacgtcggcc cacctttgga cggcatcggc
     2221 atccgaggcg tcccgaggct gtgcgaggac atcctcgacc ccagtcgcaa cgtcgccgcg
     2281 gacttccgac agagcacctt cgtcctgagc gacggcagcg tcgtcgccgg catcccgcgc
     2341 cgcaccgaag gccagacgat tgtcgtcgcc gactccaccg gcaaggaagt gtcgatcgag
     2401 aaatcgaaga tcacccgcca ggccgagtca aagctctcgc tcatgcccag caacttc
//
LOCUS       DASRWS010000073         5136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3201284, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000073 DASRWS010000000
VERSION     DASRWS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3201284"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2135
                     /locus_tag="VFF72_01295"
     CDS             189..2135
                     /locus_tag="VFF72_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZV91817.1"
                     /translation="MLTALIAAGSLYFVARVSGEEVELTLIGVVVPVLTTVMVRERHP
                     RRLLVAMASIPVVAIVALLAGSYVAGDAWLGGSLFLVLIFAGFEMRRFGDLGTTLGRI
                     AYFSYFYALVLQAPFEKAPWLALFLVIGCAVAFVVRFWIVPESREKSLDSELRALRAK
                     ISVLLRQTALSISGTSSHRDSVFASLFELQDISLLIDAQLQGGSPVERALDGAVLRCT
                     FAAETLAAIGLDVAAREPAPRRCPAEAFDCLGAAIGRGEARSAASFDAVRARLCSLPD
                     DAAQRRFEQCALALTTDPPWARSGAKGPPRHLKDDQDVDRDAHRRERAELGAAFAIDD
                     HTRLAVKATVAALGATLAGRVLSAEHWYWAVIAAFIVFSRSETSGQVLARAWQRVLGT
                     MSGVAAGLIVAEAAYGHREVELVLLFAFVALGFYLYKSAYAAYAFLLTLMLAMLYGLI
                     GNDSGRLLVVRLEETLVGGVIAAVTAATVMPWPTETDAAKKSATVLRKGARLLAALGE
                     GTAAREALREVDRAMRALRQALEPVTDAIFPAPKDARRQQLRDFFSFDFCLHQLYASS
                     GEGKPGAYRAAIDRIERLADKMMAIDSGRRPRYGHEAKPGDRKMPDKAALTDDLQGDV
                     LERLLHAVEHIENESGMRFANDPG"
     gene            2299..2700
                     /locus_tag="VFF72_01300"
     CDS             2299..2700
                     /locus_tag="VFF72_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91818.1"
                     /translation="MSLILPLLCSCAWASSPGGFPPKDAAEAFARADAVFLGEVERVK
                     KDIYGYDSTAYVKVEKVWKGRSLLSPAVVVDGVGGPTYPARIFKVGETYLFYLSASDK
                     GAALRADGFLNRVLPKGEAAGDLGYLARARK"
     gene            complement(2817..3836)
                     /locus_tag="VFF72_01305"
     CDS             complement(2817..3836)
                     /locus_tag="VFF72_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV91819.1"
                     /translation="MNNRARKIFKPEFRFGLGGVPLGNEFAKHTDREAEATLEAAWAM
                     GVRYYDVAPWYGLGLAERRFGHFLHRQRRDDFLLSSKVGKILRASSSNHHAAMFPLSD
                     SPNDLVIDYTADGVRRSIEDSLQRLGVSHLDIAFVHDLSSDFAWFPKGGWEKQYEIAR
                     KGAFPALSKMRDEGIIRGWGVGVNTPDAILRVIEDADPDVCLCARQYSLIDHAEALNH
                     LFPAVRAKKVSLVIGSSLNAGFISGSPRYNYGKENYKVPPEAIAKLDRLRGAARRHGV
                     DLRTAALQFSAAASEAVALVVGAGNERQIREDWNSMQSTIPEDFWADLRQEKLIEKNA
                     PTPRT"
     gene            4133..4933
                     /locus_tag="VFF72_01310"
     CDS             4133..4933
                     /locus_tag="VFF72_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV91820.1"
                     /translation="MGIQKRNNVMTGGEGPATMIFAHGFGCDQNMWRYLAPAFGGRFK
                     TVLFDLVGSGMSDLSAYDKAKYGSLHGYAADLLEIVDEFADGPVVLVSHSVSSTIGML
                     ATIQAGDRFDAHVMVGPSPCYINDGDYVGGFTRADIDGLLETLESNYLGWSSTMAPVI
                     MGAPDRPELGIELTNSFCRTDPDIAKHFARVTFLSDHRQDVPKATTPALILQSTDDLI
                     APLAVGEYLERMLPSATLEVIDNVGHCPHLSAPSASVSAMERFLQRSR"
     gene            4948..>5136
                     /locus_tag="VFF72_01315"
     CDS             4948..>5136
                     /locus_tag="VFF72_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZV91821.1"
                     /translation="MDAPQFDALYGQAACGLLLTTPDGTIRRANATFCSWVGFAAAEL
                     VDIRRVQDMLTIGGRVFHQ"
BASE COUNT          821 a         1677 c         1702 g          936 t
ORIGIN      
        1 atcttgccgg cgagatgtct tcgatcgttc gggtcgcgcc ttcagcgagg atgccgtcga
       61 gccgttgcat gagtccgtcg ccagaatcga gccgaccccg gttgcctctg cttgcgtggg
      121 ctcgctggcg tgagcgcctc atcgcttccg atcccggcct tttccgcctg cgcgcgagcc
      181 ttcgggcctt gctgacggca ttgatcgccg ccggatcact gtatttcgtc gccagggtgt
      241 caggcgagga ggtcgagctc acgctgatcg gcgttgtcgt acccgtcctg acgacggtga
      301 tggtgcgcga gcggcacccg cgaaggctgc tggtggcgat ggcatcgata ccggtcgtcg
      361 ccatcgtcgc gcttcttgcg ggcagctatg tcgcgggcga tgcgtggctc ggcggatcgc
      421 tgtttctggt cctgatcttc gccggattcg agatgcgacg attcggcgac ctcgggacca
      481 cactgggaag gatcgcctac ttctcgtatt tctacgccct cgtcctgcag gcgcccttcg
      541 agaaggctcc ctggctcgcc ctgttcctgg tcatcggctg cgccgtggcg ttcgtggtcc
      601 gcttctggat cgttccggag tcgcgggaga agtcactgga cagcgagctt cgcgcactgc
      661 gagcgaaaat ctcggtgctg ctccgccaga ccgcactttc gatatccggc acaagcagcc
      721 atcgcgacag cgtcttcgcg tcgcttttcg aactgcagga catctcgctt ctgatcgacg
      781 cgcaattgca gggcggcagt cccgtcgagc gggcgctcga cggcgccgtc ttgcgatgca
      841 ccttcgccgc cgagacgctc gccgccatcg gactcgacgt cgcagcccgc gagcccgcgc
      901 cgagacgatg tccggccgaa gcgttcgact gccttggcgc ggcgatcggc cggggcgaag
      961 cccgaagcgc ggcatcgttc gatgccgtcc gcgcgagact gtgctcgttg ccagacgatg
     1021 ccgcccagcg acgtttcgag caatgcgcgc tggcgttgac aaccgatccg ccgtgggcca
     1081 gatcgggcgc caagggtccg ccccgccacc tgaaggacga tcaggatgtc gatcgcgacg
     1141 cccatcgccg tgagcgcgcc gagctcggcg cggcctttgc gatcgatgac catacgcgtc
     1201 tggccgtcaa ggcgacagtg gccgcgctcg gagcgacgct tgccggacgc gtcctttcgg
     1261 cagagcactg gtactgggcc gtcattgccg ccttcatcgt cttctcgcga tccgagacat
     1321 ccggtcaagt cctggcgcgc gcctggcagc gcgtcctcgg cacgatgagc ggcgtggctg
     1381 ccggcctcat cgtcgccgag gccgcgtacg gccaccgcga ggtcgagctc gttcttctgt
     1441 ttgcgttcgt cgcactgggc ttctatctct acaagtcggc ctatgcagcc tacgcgttct
     1501 tgctgacgtt gatgctggcc atgctgtacg gactgatcgg aaacgacagc ggtcgtctgc
     1561 ttgtcgtgag actcgaggaa acgcttgtcg gaggcgtcat cgcggctgtc actgcggcaa
     1621 ccgtgatgcc ctggcccacg gagaccgacg cggcgaaaaa gagcgcgacg gtgctccgca
     1681 agggagcccg gcttctcgcg gcactcggcg agggcacggc ggcgcgggag gcgctgcgtg
     1741 aagtcgaccg cgcgatgcgc gcccttcgtc aagcgctgga accggtgacc gacgcgatct
     1801 tcccggcacc gaaggatgcg cgtcgccagc agttgcgaga cttcttcagc ttcgacttct
     1861 gccttcacca gctgtatgca tcgtcggggg aaggcaagcc cggtgcctat cgcgccgcga
     1921 tcgaccggat cgaacggctc gccgacaaga tgatggcgat cgactcaggc cgacggccac
     1981 gctacggcca cgaggcgaag cccggagacc ggaaaatgcc cgacaaggcg gccctgacag
     2041 acgacttgca aggcgacgtg ctggaacgcc tgctccacgc cgtcgaacac atcgaaaatg
     2101 aatcgggcat gcgtttcgcg aacgaccccg gctgatcgac ggcggaggcc gacaacagta
     2161 acgaggcggc agggggccgc ctcgctcgac cgcatgccgt ctgtcaggct tcgccgttgg
     2221 gtcggcgctg cgcgatttga aactcaggcg ccgtggcagt gcggcgggct gtcggggagg
     2281 accatggcga cgatgaggat gagcttgatc ttgccgctct tgtgttcgtg cgcatgggcg
     2341 tcatcgcccg gcggctttcc tcccaaggac gccgcagaag ccttcgccag ggcggatgcc
     2401 gtattccttg gcgaggtgga aagggtgaaa aaggatattt acggctacga ctccaccgcc
     2461 tacgtcaagg tggagaaggt ctggaaagga agaagccttc tttcgcctgc cgtcgttgtc
     2521 gacggcgtcg gcggaccgac gtacccggcc cggatcttca aggtcgggga gacgtatctc
     2581 ttctatcttt cggcctccga caagggagct gcccttcgag ccgatggctt tttgaatcgc
     2641 gtattgccga aaggcgaggc cgccggcgat ctcgggtacc tggccagggc gcgcaagtag
     2701 cggcagaaga tcgcagctcc tgctcacccg ttccgcgaac ctgccgacga agccgcgggc
     2761 ttcgtcgcgg aggcccttcg gcgatgacga ttcagagtcg gcgacctcgc gctttctcag
     2821 gtccggggcg tcggagcgtt cttctcgatc agcttttcct gcctcaggtc ggcccagaaa
     2881 tcctccggga tcgtcgactg catcgaattc cagtcctccc ggatctgccg ttcgtttccg
     2941 gcgcccacga cgagcgccac ggcctcggac gcggcggccg agaattgcag cgccgcggtc
     3001 cgcaagtcga cgccgtgacg tcgcgctgcg ccacgcaatc ggtcgagctt ggcgatcgcc
     3061 tcgggcggta ccttgtaatt ctccttcccg tagttgtagc gcgggctgcc cgagatgaaa
     3121 ccggcgttca gggacgagcc gatgaccagg gacactttct tcgcccgcac cgccggaaag
     3181 aggtggttga gggcctcggc atggtcgatc agcgagtatt ggcgcgcgca caggcagaca
     3241 tccgggtcgg cgtcctcgat cacccggagg atcgcgtcgg gtgtgttcac gccgacgccc
     3301 cagccgcgga tgatcccctc gtcgcgcatc ttcgaaagcg caggaaatgc acccttgcgc
     3361 gcgatctcgt actgcttctc ccagcctccc ttgggaaacc acgcgaaatc ggacgacaga
     3421 tcgtggacga aggcgatgtc gaggtgcgaa acgcccaggc gctgcaggct gtcttcgatc
     3481 gagcgacgga cgccgtcggc ggtgtagtcg atgacgaggt cgttgggcga gtcggagagc
     3541 ggaaacatcg ccgcatggtg attgctgctc gacgcgcgga ggatcttgcc gaccttcgat
     3601 gagagcagga agtcgtcccg ccgctgccgg tgcaggaagt ggccgaagcg gcgctctgcc
     3661 aggcccaacc cgtaccaggg cgcgacgtcg tagtagcgaa cgcccatcgc ccaggcggcc
     3721 tccagcgtcg cctccgcctc cctgtccgtg tgcttggcga actcgttgcc gagcggcacg
     3781 ccgccgagtc cgaaccggaa ctcgggcttg aagatcttcc gggcgcggtt gttcatgtcg
     3841 aggtcgtttc gatcggcggg atcggccagc cgaaaatggt agggacgagc caaggccctt
     3901 gtcgtggtcg gcgggcccgc cgtcgcgtag gcgctcgccc cgttcgtggc cggagcgagc
     3961 ccgcggcaat cgggcagcat cgtgcgctgc gtgcgctgcg tgcgctgcgt gcgctgagtg
     4021 cgacagttcg cgcctgcaat cgacacgctt cgaacgaccg cgccacgagg ttcgtcgccg
     4081 cgacgctgac ctgctgctga cgcgcaatgg cgtcgcctgc cagaatcggg tcatgggcat
     4141 ccagaagcgc aacaacgtca tgaccggggg cgagggcccg gcgacgatga tcttcgcgca
     4201 cgggttcggt tgcgaccaga acatgtggcg ctacctcgcg ccggcgttcg gcggccgttt
     4261 caagaccgtg ctcttcgatc ttgtcggcag cggcatgtcg gacctgtcgg cgtacgacaa
     4321 ggcgaaatac ggttcgctgc acgggtacgc ggccgacctg ctcgagatcg tcgacgaatt
     4381 cgccgacggg ccggtcgtcc tggtcagcca ttccgtgagc tccacgatcg gcatgcttgc
     4441 cacgatccag gcgggcgacc ggttcgacgc ccacgtcatg gtcgggccgt cgccttgcta
     4501 tatcaacgac ggcgactacg tcggcggctt cacccgcgcc gatatcgacg gattgctcga
     4561 gaccctcgag agcaactatc tcgggtggtc gagcaccatg gcgccggtca tcatgggtgc
     4621 gccggatcgt ccggagctcg gcatcgagct cacgaacagc ttttgcagaa ccgacccgga
     4681 catcgccaag cacttcgccc gcgtgacctt cctgtccgac catcggcaag acgttccgaa
     4741 ggcgacgacg ccagcgctca tcctgcagtc gacggacgac ctgatcgcac ctttggcggt
     4801 cggcgaatac ctggagcgga tgctgccctc cgcgacgctc gaggtcatcg acaacgtcgg
     4861 ccattgtccg cacctgagtg cgccaagcgc gagcgtctcg gcgatggagc gattcctcca
     4921 gcgctcgcgc tgaggacgga aggcgcgatg gacgcgccgc agttcgacgc gctctacggg
     4981 caggccgctt gcggcctctt gctgacgacg cccgacggaa cgatccgccg cgccaatgcc
     5041 acgttttgtt cctgggtcgg tttcgccgcc gcggagctcg tcgacatccg tcgagtccag
     5101 gacatgctga cgatcggcgg gcgcgtcttt caccag
//
LOCUS       DASRWS010000074         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_32444, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000074 DASRWS010000000
VERSION     DASRWS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32444"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFF72_01320"
     CDS             complement(<1..682)
                     /locus_tag="VFF72_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91822.1"
                     /translation="MRGSPGEADEFDIKRMLRRTVVLPGLPGPVMYRVIRRVGDPVWT
                     VTNGTLEPLGGPFPDPKDGESSDKFAVDGATGRIFGVNRQGEVFVAEPGTKDFRRLHS
                     PYEDWDPFPGMPARPNQNKRPPFSGLLTYLPERQTVLFGFSTSTNRIEGDKLVQEDRT
                     SLYRIEGEKFVLVSDKLPFRFPIQIDTLPKLGALLVTSANNGMAILHDNGTIEIVREP
                     VLSEPGVHP"
     gene            complement(891..1613)
                     /locus_tag="VFF72_01325"
     CDS             complement(891..1613)
                     /locus_tag="VFF72_01325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZV91823.1"
                     /translation="MATLPRAILFDLDDTLIRAYAQPEEAWTRLLHFFAAHLDAHDAA
                     AIERVRVAIMEEARAFWNDRAEAAKWRLNIPGARRLSVRRGLARPGYTDEALADHIAD
                     AFTELRRAEYRLYPDAHATVDALRRRGVKLALVTNGAAEFQRAKIERFDLSHRFDHIQ
                     IEGEFGMGKPEPDVYRHALERLGCDACDAWMVGDNYEWEVEAPQKLGLCGIWYDPFDA
                     GIPAHATAKPTRVIKRLGELLE"
     gene            complement(1630..>2222)
                     /locus_tag="VFF72_01330"
     CDS             complement(1630..>2222)
                     /locus_tag="VFF72_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91824.1"
                     /translation="SHVAVRGGTVVASETYNEGSDISIAAKRLADATKGEGKLAILLP
                     IAPPRLNTVLASLSANGVDPKSVQLIGTGVWDTPGIGSDPMLRGAWYAAPDPARRADF
                     ERKYVATYGRPPQRLATLAYDAVSLAAQLARLKPGGDFSAEAIANPNGWSGVDGVFRL
                     LPDGRTERALAVIEVQGDRGVVVSPAPTSFTSSQTN"
BASE COUNT          334 a          728 c          793 g          367 t
ORIGIN      
        1 ggggatgcac cccgggctcg ctcagcaccg gttcgcgaac gatctcgatc gtgccgttgt
       61 cgtgaaggat cgccatgccg ttgttggcgc tcgtgaccag caaggcgccg agtttcggca
      121 gcgtgtcgat ctgaataggg aagcgaaacg gcagcttgtc gctcacgagc acgaatttct
      181 cgccctcgat ccgatagagc gaggtgcggt cctcctgcac gagtttgtcg ccctcgatcc
      241 ggttggtcga cgtgctgaac ccgaacagga ccgtttgtcg ttccggcagg taggtgagca
      301 agccgctgaa aggtgggcgc ttgttctggt ttgggcgggc gggcatgcct ggaaaagggt
      361 cccagtcctc gtaagggctg tgcagccggc gaaaatcctt cgtgccgggc tccgccacga
      421 agacctcgcc ttggcggttg acgccgaaga ttcgaccggt cgcgccgtcg acggcgaatt
      481 tgtcgctgct ctcgccgtcc ttcgggtccg ggaaggggcc gccgagcggc tcgagcgttc
      541 cattggtgac cgtccagaca gggtcgccga ctcttcggat gacgcgatac atcacggggc
      601 cgggcagacc cggcagcacc accgtccgcc gcagcatccg cttgatgtcg aactcatccg
      661 cttcgccggg gctgccgcgc acgaccggca ggacgcagag atcggccgag cgggcgaagt
      721 cggccgcggc cgctgcgccc gccaggaaaa gggcgaggac ggcgccgaca agggacgcga
      781 tgacgttgcg cgggatcaca ccagataccg tccactgcag gagttctgca gtggacgatg
      841 cgcctggact ccggtcaaac cggagattgt aacgtctgta ggccctcaac ctactccagc
      901 aactcgccca gccgcttgat cacgcgcgtc ggcttggccg tggcgtgtgc gggaatgccg
      961 gcgtcgaacg ggtcgtacca gatgccgcac aggccgagct tctgcggcgc ctcgacttcc
     1021 cattcgtaat tgtcgccgac catccacgcg tcgcaggcgt cgcagcccag ccgttcgagg
     1081 gcgtggcggt agacgtcggg ttcgggcttg cccatgccga actcgccctc gatctggatg
     1141 tggtcgaagc ggtggctgag gtcgaagcgc tcgatcttgg cgcgctggaa ttcggccgcc
     1201 ccgttggtca ccagagcaag cttgacgccg cgccggcgca aggcgtcgac cgtggcgtgg
     1261 gcgtcgggat agagccggta ctcggcgcgg cgcaattcgg tgaaggcgtc ggcgatatgg
     1321 tcggccagcg cctcgtcggt gtagccgggc cgcgccaggc cacgcctcac cgacagccgc
     1381 cgcgcgcccg ggatgttgag ccgccacttg gccgcttcgg cgcggtcgtt ccagaaggcg
     1441 cgcgcctcct ccatgatcgc gacccgcacg cgctcgatcg ccgcggcgtc atgggcgtcg
     1501 agatgggcgg cgaagaagtg caacaggcgc gtccacgcct cttccggctg cgcgtaggcc
     1561 cggatcagcg tgtcgtcgag atcgaacagg atggcgcgag gaagcgtcgc catcagttgg
     1621 tttgccccgt cagttcgttt gtgacgaggt gaagctggtc ggcgccgggc tgaccaccac
     1681 gccgcgatcg ccctggacct cgatcaccgc cagggcgcgc tcggtgcggc cgtcgggcag
     1741 caggcggaac acgccgtcga cgcccgacca gccgttgggg ttggcgatcg cctcggccga
     1801 gaagtcgccg cccggcttca gccgcgccag ctgggcggcg agcgagacgg cgtcgtaggc
     1861 gagcgtcgcc agccgctgcg gcgggcggcc ataggtcgcg acgtacttgc gctcgaagtc
     1921 ggcgcggcgc gccggatcgg gcgcggcgta ccaggcgccc cgcagcatcg ggtccgagcc
     1981 gatgcccggc gtgtcccata caccggtgcc gatcagctgc accgacttgg ggtcgacgcc
     2041 gttggcgctg agcgaggcca gcacggtgtt gagcctgggc ggcgcgatcg gcagcaggat
     2101 ggcgagcttg ccttcgccct tggtggcgtc cgcgagccgc ttggcggcga tcgagatgtc
     2161 gctgccttcg ttgtaggtct cggaggcgac caccgtgccg ccgcgcaccg cgacatggct
     2221 ct
//
LOCUS       DASRWS010000075         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_321849, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000075 DASRWS010000000
VERSION     DASRWS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321849"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFF72_01335"
     CDS             <1..526
                     /locus_tag="VFF72_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HZV91825.1"
                     /translation="GRLMFQFFHVERGDGLRERPVIELVTFERGRADEASAQALGLKA
                     GDPVATFENVLKLQGRAVIHDRIVVAAALFRGLNERRLRDRPGTIYQYYQAEFGITVL
                     RAHERARAVAADRSAARALGIAAGTPVIEVRRTALSFNDRPVELRVSVIDTTRHDYVN
                     TLSRPAAPAAAALP"
     gene            537..1448
                     /locus_tag="VFF72_01340"
     CDS             537..1448
                     /locus_tag="VFF72_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZV91826.1"
                     /translation="MNKPAPRPAKRSFPSAHEDAIKETTSPSARYEGPESSYRLAFMD
                     TQFLLRDELRPVRVQLELLKPQLIQDEHEIEQTIVIFGSARIVAPDVAERELAEARAG
                     GDDKKLRFAESRAAMSRYYEEARRFSAIVTQRSATLDKPIYVVTGGGPGIMEAGNRGA
                     FEVGGKSLGMNIVLAHEQVPNAYITPELCFQFHYFALRKMHFLMRAVALVCFPGGFGT
                     LDELFEVLTLTQTGKSRMRPILLFGREFWSRLIDFDLLIETGMISPGDVNLFHFVESA
                     EEAWAMLAAEYGYDLPETTTGDLAVDI"
     gene            1579..>2313
                     /locus_tag="VFF72_01345"
     CDS             1579..>2313
                     /locus_tag="VFF72_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZV91827.1"
                     /translation="MTSRRRISLIGAPTDIGAGSRGSSMGPEALRVANIGPVLEGHGF
                     EVIDRGNLAGPPNPWQPPTDGYRHLDAVAEWNRTVHEAVHDELAAGRFPLLLGGDHCL
                     GIGSISAVARHCRANQRKLRVLWLDAHADFNTNALTPSGNIHGMPVAVLCGYGPPELT
                     GIGGAVPAISPKQVRQIGIRSVDAGEKKLVHEAGLEVFDMRYIDEMGMRHTMELALAT
                     LDANTHLHVSFDVDFLDADVAPGVGTT"
BASE COUNT          362 a          853 c          766 g          332 t
ORIGIN      
        1 cggccgcctc atgttccagt tcttccacgt cgagcgcggc gacggcctgc gtgaacgccc
       61 ggtcatcgag ctcgtcacgt tcgagcgcgg ccgggccgac gaggcgtcgg cgcaggcgct
      121 cgggctgaag gccggcgacc cggtggcgac gttcgagaac gtcctcaagc tgcagggccg
      181 cgccgtcatc cacgaccgca tcgtcgtcgc cgcggccctc tttcgcggcc tcaacgaacg
      241 ccggctgcgc gaccgccccg gcaccatcta ccagtactac caggccgagt tcggcatcac
      301 ggtgctgcgc gcccacgagc gggcccgcgc cgtggcggcc gaccggagcg cggcgcgcgc
      361 gctgggcatc gccgcgggca cgccggtgat cgaggtgcga cgcaccgccc tcagcttcaa
      421 cgatcggccg gtcgagctgc gcgtctcggt catcgacacg acgcgccacg actacgtgaa
      481 caccttgtcc cgtcccgcgg cgccggcggc cgcggcgttg ccgtaagctt cacgcgatga
      541 acaagccagc cccccgcccg gcgaagcgga gctttccgtc ggcgcacgaa gatgccatca
      601 aggaaaccac cagcccgagc gcccgctacg aaggcccgga aagctcctac aggctggcgt
      661 tcatggacac ccagttcctg ctgcgcgacg agcttcggcc ggtccgcgtg cagctcgagc
      721 tgctcaagcc gcagctgatc caggacgagc acgagatcga gcagacgatc gtcatcttcg
      781 gcagcgccag gatcgtcgcc cccgacgtgg ccgagcgcga gctggcggaa gcgcgtgccg
      841 gcggcgacga caagaagctg cgcttcgccg agtcgcgtgc ggcgatgtcg cgctactacg
      901 aggaggcccg gcgcttttcg gcgatcgtca cgcagcggtc ggcgacgctc gacaagccca
      961 tctacgtcgt caccggcggc ggccccggca tcatggaggc cggcaatcgc ggcgccttcg
     1021 aagtcggcgg caagagcctg ggcatgaaca tcgtgctggc ccacgagcag gtgcccaacg
     1081 cctacatcac gcccgagctc tgctttcagt tccactactt cgcgctgcgc aagatgcact
     1141 tcctgatgcg ggccgtggcg ctggtgtgct ttccgggcgg cttcggcacc ctcgacgagt
     1201 tgttcgaggt gctgacgctg acgcagaccg gcaagtcacg gatgcggccg atcctgctgt
     1261 tcggccgcga gttctggagc cggctgatcg acttcgactt gctcatcgag accggcatga
     1321 tctctccggg cgacgtcaac ctgttccatt tcgtcgagtc ggccgaggag gcctgggcga
     1381 tgctggccgc cgagtacggc tacgacctgc ccgaaacgac gacgggcgat ctggcggtcg
     1441 acatctgagg ccgatggtgc ccgcgcgctc acgagtccgt tcgctgccgc cggcctcgga
     1501 agcgacccgg cgcccggccc gcgtgcgccc atcctcacgc ccacgagtcc gttcgccgcg
     1561 caccgggagg gcgtcgcggt gacgtcgagg cggcgcatca gcctgatcgg cgcgccgacc
     1621 gacatcggcg ccggcagccg cggctcgagc atggggcccg aggcgctgcg cgtggccaat
     1681 atcgggccgg tactcgaagg ccacggcttc gaggtgatcg accgcggcaa cctcgccggt
     1741 cctcccaacc cctggcagcc gccgaccgac ggctaccggc atctcgacgc agtggccgaa
     1801 tggaaccgca ccgtgcacga ggccgtccac gacgagctcg ctgccggacg cttcccgctg
     1861 ctcctcggtg gcgaccactg cctgggcatc ggctcgatca gcgcagtggc gcgccactgc
     1921 agggccaacc agcgcaagct gcgcgtgctc tggctcgatg cgcacgccga cttcaacacc
     1981 aacgccctca cgcccagcgg caacatccac ggcatgccgg tggccgtcct ctgcggctac
     2041 ggcccccccg agctcaccgg catcggcggc gcggtgccgg cgatcagccc gaagcaggtc
     2101 cgccagatcg gcatccgcag cgtcgacgcc ggcgagaaga agctcgtcca cgaagccggc
     2161 ctcgaggtct tcgacatgcg ctacatcgac gagatgggca tgcgccacac gatggagctg
     2221 gcgctcgcca cgctcgacgc caacacgcat ctgcacgtga gcttcgacgt cgactttctc
     2281 gacgccgatg tggcgcccgg cgtcggcacc acc
//
LOCUS       DASRWS010000076         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3202001, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000076 DASRWS010000000
VERSION     DASRWS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202001"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFF72_01350"
     CDS             complement(<1..455)
                     /locus_tag="VFF72_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912208.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV91828.1"
                     /translation="MTPARVVPLRRPGLWLSSAIVVVLAAALAISVWNNPHIDHAAIA
                     QFQFAPAILAGLRLTIVLALLAALIGLVLGVVLAIMRLSASPVLRLFSGTYTWLLRGT
                     PLLVQILLWGNLALLFQHIGPFDTNRLITPFVASVIALGLNEGAYMAEIV"
     gene            complement(474..1331)
                     /locus_tag="VFF72_01355"
     CDS             complement(474..1331)
                     /locus_tag="VFF72_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91829.1"
                     /translation="MSHHSPAPAWRRPAFVSLLATALVGLAATTHAGAADDIRAGSLT
                     NSPPMISYAADGTTLQGMIVDLAAAMSKQMGRTIVFKPMAFPAIIPGLEAGSIDIAFT
                     VMNDTPEREKVIDFVDFYNFGTKLLVKKGNPEHVESLETACGKTVSTVQGSTQIKLAE
                     DMSAGCTKAGKPPITVMQYAQPSDARLQVQTGRVAAFLGNSPVMVYLAKTAGDGTVFD
                     VVAGHEYQPVPIGIGVPKKSTALRDELKKALDSLIADGTYMKILQKHGLEGGNIGKAE
                     INGGRNVKI"
     gene            complement(1400..2818)
                     /locus_tag="VFF72_01360"
     CDS             complement(1400..2818)
                     /locus_tag="VFF72_01360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZV91830.1"
                     /translation="MTSGRRSLRARMVWRVLPPLAATWLIGSAIALSLSWIFAGQAYD
                     RALLENAYAIAANIGARNGQPVLDLTAREIDNLLFDSNEKQYFVVLGAGGHVLAGNAE
                     LVSATTTNGPAREFSNGSYRGEPVRLATLRGDRPAPHVVIVGQTTRSRTRLLRAMLLR
                     SIAPQVVLLLLLGAYLWSQIARELAPLTVLQRELDRRDSGDLGPVRLAPMSEDVERLS
                     DAINALLSRIARGVQAQREFAGNVAHDLRTPLAGIRALAEYGLARDDPAIWRQQLASI
                     VASEERASRLVEQLLALALADEARDSIRLEVLAVDELVRRVLLGFMPRADATGADLGA
                     TGLDVSVHAWASPALLEGVLINLIDNALRYGRSGDAATITVEVECSGETVEIAVVDQG
                     PGIDPPQRDRQPRRWEHGPSTGEAGAGAGAGLGLAIAVRYAELLQGSLRLEDGPEGRG
                     LRAVLALRASRNVRIAPVEPVV"
     gene            complement(2815..>3228)
                     /locus_tag="VFF72_01365"
     CDS             complement(2815..>3228)
                     /locus_tag="VFF72_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZV91831.1"
                     /translation="GADDYLGKPFELGELAARLRALVRRARGVEALTDVGGLRLDRST
                     GRFFIAGGGACEATELELPAREHAVLVELMTPPGRVVSKRALSGKLSSFDEHLGDNAL
                     EAFVSRLRKKLLGSGSTIRTLRGLGYLIEAERTVP"
BASE COUNT          469 a         1106 c         1180 g          473 t
ORIGIN      
        1 acgatctcgg ccatgtaggc gccctcgttg aggccgaggg cgatcaccga ggcgacgaag
       61 ggcgtgatca ggcggttggt gtcgaaggga ccgatgtgct ggaagagcag cgcgaggttg
      121 ccccatagca ggatctggac gagcagcggc gtgccgcgca gcagccacgt gtaggtgccg
      181 ctgaagaggc gcagcaccgg gctcgccgac agccgcatga tcgccaggac gacgcccagg
      241 acgaggccga tcagcgccgc cagcagggcc aggacgatcg tcaggcgcag ccccgcgagg
      301 atcgccggcg cgaactggaa ttgcgcgatc gccgcgtggt cgatgtgcgg gttgttccag
      361 accgagatcg ccagcgccgc ggcgagcacg acgacgatcg cgctgctcag ccacaggccg
      421 ggccggcgca gcggaacgac gcgggccggc gtcatcgcgg ccacgcccgg ggcctagatc
      481 ttgacgttcc tgccgccgtt gatctcggcc ttgccgatgt tgccgccctc gaggccgtgc
      541 ttttgcagga tcttcatgta ggtgccgtcg gcgatcagcg agtcgagcgc cttcttcagc
      601 tcgtcgcgca gcgcggtgct ctttttcggc acgccgatgc cgatcggcac gggctggtac
      661 tcgtgcccgg cgacgacgtc gaagacggtg ccgtcgccgg ccgtcttggc caggtagacc
      721 atcaccggcg agttgccgag gaaagcggcg acgcggccgg tctgcacctg cagccgcgca
      781 tccgaaggct gggcgtactg catgaccgtg atcggcggct tgcccgcctt cgtgcagccg
      841 gcgctcatgt cctcggcgag cttgatctgg gtcgacccct ggaccgtcga gacggtcttg
      901 ccgcaggcgg tctcgaggct ctcgacgtgc tcgggattgc ccttcttcac cagcagcttg
      961 gtgccgaagt tgtagaagtc gacgaagtcg atcaccttct cgcgctcggg cgtgtcgttc
     1021 atcaccgtga aggcgatgtc gatgctcccg gcctcgagcc ccgggatgat cgccgggaag
     1081 gccatcggct tgaagacgat ggtgcggccc atctgcttgc tcatcgccgc ggccaggtcg
     1141 acgatcattc cctgcagcgt cgtgccgtcg gccgcgtacg agatcatcgg cgggctgttg
     1201 gtgagcgagc cggcgcggat gtcgtcggcc gcgccggcat gggtcgtggc agcgagcccg
     1261 accagcgccg tcgcgaggag ggagacgaac gcggggcgtc gccaggcggg ggcgggggag
     1321 tgatgcgaca tgtcaatcgt ctcctgatgg tcgtgcgagc gcaaggatca gcgcccgggg
     1381 ggcgcgacag cacccggttc tacaccaccg gttcgaccgg ggcgatccgt acattccgcg
     1441 acgcgcgcag ggccagcacc gcgcgcaggc cgcgtccttc cggaccgtct tcgagtcgca
     1501 ggcttccctg cagcagctcg gcgtagcgca cggcgatcgc gagtccgagc ccggctccgg
     1561 ccccggcgcc ggcctcgccg gtggacgggc cgtgttccca gcgccgaggc tgacggtcgc
     1621 gctgtggcgg atcgattccg gggccctggt cgacgacggc gatctcgacc gtctcgccgc
     1681 tgcattcgac ctcgacggtg atcgtcgcgg cgtcgccgct gcggccgtag cgcagggcgt
     1741 tgtcgatcag gttgatgagc acgccttcga gcagcgccgg tgacgcccag gcgtggaccg
     1801 acacgtcgag gcctgtcgcg ccgaggtcgg cgccggtcgc gtcggcgcgc ggcatgaagc
     1861 cgagcaggac gcgacgcacc agctcgtcga cggcgaggac ctccaggcgg atgctgtcgc
     1921 gcgcctcgtc ggccagggcc agcgcgagca gctgctcgac cagccggctg gcgcgctctt
     1981 cgctggcgac gatgctggcg agctgctgtc gccagatcgc cgggtcgtcg cgagccaggc
     2041 cgtattcggc cagggcgcgg atgccggcga gcggcgtcct caggtcgtgg gcgacgttgc
     2101 cggcgaattc acgctgcgcc tgcacgccgc gcgcgatgcg cgacaggagg gcattgatcg
     2161 cgtcgctcag ccgctcgacg tcctcggaca tcggcgccag ccgcaccggc ccgaggtcgc
     2221 ccgagtcgcg ccggtcgagc tcgcgctgca gcaccgtcag cggcgccagc tcgcgggcga
     2281 tctgcgacca gagatacgcg cccagcagca gcaggagaac gacctgcggc gcgatcgagc
     2341 gaagcagcat ggcccgcagc aaccgggtgc ggcttctcgt cgtctggccg acgatgacga
     2401 catgcggcgc cggccggtcg ccgcgcagcg tcgccaggcg gaccggctcg ccccggtagc
     2461 tgccgttcga gaattcgcgg gccgggccgt tcgtcgtcgt cgcggacacg agctcggcgt
     2521 tgccggcgag gacgtggcct ccggcgccga ggaccacgaa atactgcttc tcgttgctgt
     2581 cgaagaggag gttgtcgatc tcgcgcgccg tcaggtcgag caccggctgg ccgttgcgcg
     2641 cgccgatgtt ggcggcgatg gcgtaggcgt tctcgaggag ggcacggtcg taggcctggc
     2701 cggcgaagat ccacgacagc gacagcgcga tcgcgctgcc gatcagccac gtcgccgcaa
     2761 gcggcggcag cacgcgccag accatgcgcg cgcgcagcga gcggcggccg ctcgtcacgg
     2821 aacggtccgt tcggcctcga tcaggtagcc gagcccgcgc agggtccgga tcgtcgagcc
     2881 gctgccgagc agcttcttgc gcagccgcga gacgaacgcc tcgagggcgt tgtcgccgag
     2941 atgctcgtcg aacgacgaga gcttccccga cagcgctcgt ttgctgacga cgcggccggg
     3001 cggcgtcatc agctcgacca ggaccgcgtg ctcgcgcgcc ggcagctcga gctccgtcgc
     3061 ctcgcaagcg ccgccgccgg cgatgaaaaa gcgcccggtc gagcgatcga ggcgaaggcc
     3121 gccgacgtcg gtcaacgcct cgacgccgcg cgcccgtcgc accagcgccc gcaatcgcgc
     3181 cgcaagctcg cccagctcga agggcttgcc gagatagtcg tcggcgcc
//
LOCUS       DASRWS010000077         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1760268, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000077 DASRWS010000000
VERSION     DASRWS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760268"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1779)
                     /locus_tag="VFF72_01370"
     CDS             complement(205..1779)
                     /locus_tag="VFF72_01370"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HZV91832.1"
                     /translation="MTGDVRAGEPRQEASPIRPAEVALLASVFIVAACGLVYELAAGT
                     LASYLLGDSILQFSTVIGTYLFAMGLGSWLSRFLERQLVAQFLRVELLVGVVGGLTPA
                     ALFALQSASTPSFRFALYGLVLAVGILVGLEIPLVMRILRRYAGRSNHGGLKVLVSQV
                     LTFDYLGALVVAIAFPLLLVPHLGLIRTGALFGLMNAGVALWALCLFRGELRGVGRQA
                     LACVLATAALGAAFVFADRVTSWSEDRFYADHVVYSESSPYQRIVITASHAGIRLFLN
                     GNLQFYSRDEYRYHEALVHPALSATIAPRRVLVLGGGDGMAAREILKYPSVESVTLVE
                     LDPHMTRLFSTEPMLRALNHDSLRSPRLHVVNADAFGWLERNDAMFDAIVVDFPDPTN
                     FSIGKLYTKTFYELVDAHLSASGYAVIQTTSPLIARRSFWTVVTTLEAAGLTTAPYHA
                     HVPSFGEWGFIIASHRPFVLPRNLPPGLRFLDVAGLPALFQFPPDMARVPTEVNRLSN
                     QVLVQTFDEEWGKMAR"
     gene            complement(1918..2139)
                     /locus_tag="VFF72_01375"
     CDS             complement(1918..2139)
                     /locus_tag="VFF72_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HZV91833.1"
                     /translation="MGLEWLKPEIVFGSILYAVIGVVIFWLCFVIVDKLTPYHLWDEI
                     VHKKNTALAIVVAAMAIAIGMIVAAAVHG"
     gene            complement(2183..3796)
                     /locus_tag="VFF72_01380"
     CDS             complement(2183..3796)
                     /locus_tag="VFF72_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HZV91834.1"
                     /translation="MATEPTPQRAYRAACPNCGAPVEFRSAATPFAVCSFCHSTVVRA
                     GDQLRKIGESAEVFDDHSPLQLGAAGKYQGAPFTLIGRLQYQYAEGTWNEWHALFESG
                     PDLQKSGWLSEDNGRFVIAFDAPLQTPLPPPEQLQVGAPLTVNGESWLVASVVAAKLI
                     AAQGELPRRPNLDHGFVVADVRSGRGDVGTIDYTDPARPSWSVGHSVSLSELAMTGLA
                     ESSEKTLSGQSLQCPNCGAALEVKLATTQSIVCHQCKSVIDVSKGIGGDLAHYQQSNS
                     HDPQIPLGTVGTLAFGGKQALPWQVVGYAEYVEIPDSADDEQSFWREYLLYHRIEGFA
                     FIVDADDGWSWTIPLTGAPQSFGESVKYQDVLYRKLYDYTGQVTYVLGEFYWRLAKDQ
                     RTLNSDYQGTGANSARRLNRERTHGEGNEEVVWSGGETLSADTVVAAFRLKPEQRAAL
                     QRDALPTAFHSSSPLAKIIFWIFIIFLILLVVRCSESSGPGDCTQTRNTFGAASQEYQ
                     RCLASQRSGTGFRTGGGAFGGFSTGGGHK"
     gene            complement(3859..>4182)
                     /locus_tag="VFF72_01385"
     CDS             complement(3859..>4182)
                     /locus_tag="VFF72_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZV91835.1"
                     /translation="YQTAQSIPAFAAGAGGDGGGGGIVGSAVGLGAGVALGQVMANQL
                     GQGLATAAPGAPAAAAGAAAASAGVKPEDVMATLERLADFKAKGILTQEEFDAKKAEL
                     LKKLA"
BASE COUNT          713 a         1363 c         1472 g          634 t
ORIGIN      
        1 gcgtcgcgcc ggacgcaggg ggtgcacccg acttcgcgtc gggccagccg gtgcgcagca
       61 gatgaccgcg atcgacgccg gcgccgaccc agccgccgct gtaacccgcg ccgttgcgag
      121 cacatccgcc gatgccgccc agtgcgacgg ccgaacccgc ggcgagcacc gcacgacgtg
      181 tcggcccacg cgaagcagcg cgtctcatcg cgccatcttg ccccattcct cgtcgaaggt
      241 ctggacgaga acctggttgg aaagccggtt cacttcggtc ggcacgcgcg ccatgtcggg
      301 cgggaactgg aagagcgccg gcaggccggc cacgtcgagg aagcgaaggc cgggagggag
      361 gttcctgggc agcacgaacg gccggtggct ggcgatgatg aacccccatt cgccgaagct
      421 cggcacgtgc gcgtggtacg gagccgtcgt caggccggcg gcctcgagcg tcgtcaccac
      481 ggtccagaag ctcctcctgg cgatcagcgg cgaagtcgtc tggatcacgg cgtagccgct
      541 ggccgagaga tgggcatcga ccagctcgta gaacgtcttg gtgtagagct tgccgatcga
      601 gaagttggtc ggatcgggaa agtcgacgac gatggcgtcg aacatggcgt cgttgcgctc
      661 gagccagccg aaggcatcgg cgttgacgac gtgaagcctc ggcgagcgca gcgaatcgtg
      721 gttgagggca cgcagcatcg gctcggtcga gaagagccgc gtcatgtgcg ggtcgagctc
      781 caccagcgtg acgctctcga cgctcgggta cttgaggatc tcgcgcgccg ccatgccgtc
      841 gccgccgccc agcaccagca cgcggcgcgg cgcgatcgtt gccgagaggg ccggatggac
      901 cagggcttcg tgatagcggt attcgtcgcg cgaatagaac tgcaggttgc cgttgagaaa
      961 aagccggatg ccggcgtggc tggccgtgat gacgatgcgc tggtaggggc tcgattcgct
     1021 gtagacgaca tgatcggcat agaagcgatc ttccgaccag ctcgtgacgc ggtcggcgaa
     1081 gacgaaggcg gcgccgaggg ccgctgtcgc gagcacgcag gcgagtgctt gcctgccgac
     1141 gccacgcaac tcgccacgaa acaggcagag cgcccagagc gcgacgcccg cattcatgag
     1201 cccgaacagc gctccggtgc ggatcaggcc gaggtgcgga acgagcagca gcggaaaggc
     1261 gatggcgacg acgagtgcac cgaggtagtc gaaggtgagc acctgcgaca ccagcacttt
     1321 caggccgccg tggttcgatc ggccggcgta gcgcctgagg atgcgcatca ccagcgggat
     1381 ctcgaggccg acgaggatgc ccaccgccag cacgaggccg tagagggcga accggaacga
     1441 gggcgtcgag gccgactgca gcgcgaagag ggccgccggc gtcagcccgc cgaccacgcc
     1501 gaccagcaat tcgacgcgaa ggaactgggc gaccagctgg cgttcgagga agcgcgacag
     1561 ccacgagccc aagcccatcg cgaagaggta cgtgccgatg accgtcgaga actgcaggat
     1621 cgagtcgccc agcaggtagc tggcgagcgt tccggcggcg agctcgtaga cgaggccgca
     1681 ggcggcgacg atgaagaccg aggcgaggag cgccacctcg gcggggcgga tcggcgatgc
     1741 ctcctgtcgc ggctcgcccg cccggacgtc acctgtcacg gtcgcgacct tcgcagtaag
     1801 cgttgaacgg gtcgtcgcga gcgagcccac gctgcgacga caggcgcaaa cctaccggga
     1861 tgcggtcggc gccgcgatcg caggatcggc tcgtcgtgca ccggggtcat tcatggctca
     1921 tccgtgaacc gcggccgcga cgatcatgcc gatggcgatc gccatcgcgg cgacgacgat
     1981 cgccagtgcc gtgttcttct tgtgcacgat ctcgtcccag aggtgatacg gcgtgagctt
     2041 gtcgacgatg acgaaacaca gccagaagat gaccacgccg atcacggcgt agaggatcga
     2101 cccgaagacg atctccggct tcaaccattc gagtcccatt gcgtgctcct gttgtgtgag
     2161 tcgaggaagg aggtcgtcgc cgctacttgt ggccgccgcc cgtggaaaat ccgccgaagg
     2221 cgccgccgcc ggtccgaaag cctgtgccgc tgcgctggct cgccaggcat cgctggtatt
     2281 cctgcgacgc cgcgccgaag gtgttgcggg tctgggtgca gtcgccgggg ccgctgcttt
     2341 cactgcagcg caccaccagc aggatcagaa agatgatgaa gatccagaag atgatcttgg
     2401 ccagtggcga cgacgaatga aaggcggtgg gcagcgcgtc gcgttgcagc gcggcgcgct
     2461 gttcgggctt gagccggaac gccgcgacga ccgtatcggc cgagagggtc tctccgcccg
     2521 accagaccac ctcctcgttg ccctcgccgt gggtgcgctc gcggttgagg cggcgggccg
     2581 agttcgcgcc ggtgccctgg tagtccgagt tgagggtgcg ctggtccttg gcgagacgcc
     2641 agtagaactc gcccaggacg taagtcacct ggccggtgta gtcgtagagc ttgcgataca
     2701 ggacgtcctg gtacttgacg ctctcgccga acgactgcgg tgcgccggtg agcggaatcg
     2761 tccagctcca gccgtcgtcg gcatcgacga tgaaggcaaa gccctcgatg cggtgataga
     2821 ggaggtattc gcgccagaag ctctgctcgt cgtcggcgct gtccgggatc tcgacgtact
     2881 cggcgtatcc caccacctgc cagggcagcg cctgcttgcc gccgaacgcc agggtgccca
     2941 ccgtacccag ggggatctgc gggtcgtggc tgttgctctg ctggtagtgg gccagatcgc
     3001 cgccgatgcc cttcgacacg tcgatcaccg acttgcactg atggcagacg atcgactgcg
     3061 tcgtggccag cttcacctcg agtgccgcac cgcagttcgg gcattgcagc gactggcccg
     3121 agagcgtctt ctcgctcgac tcggccagcc ccgtcatcgc cagctccgag agcgacacgg
     3181 aatggccgac ggaccacgaa ggcctcgccg gatcggtgta gtcgatcgtg ccgacgtcgc
     3241 cgcggcccga acggacgtcg gcgacgacga agccgtgatc gaggttgggc cggcgcggca
     3301 gctcgccttg cgccgcgatc agcttggcgg cgacgaccga ggcgaccaac cagctctcgc
     3361 cgttgacggt gagcggcgcg ccgacctgca gttgctcggg cggcggcagc ggcgtttgca
     3421 gcggcgcgtc gaaggcgatg acgaagcgcc cgttgtcttc cgaaagccag cccgacttct
     3481 gcaggtcggg gccgctctcg aagagcgcat gccactcgtt ccaggtgcct tcggcgtact
     3541 ggtactgcaa ccggccgatg agcgtgaacg gcgcgccctg gtacttgcct gcggcgccga
     3601 gctgcagcgg cgagtgatcg tcgaagacct cggcgctctc gccgatcttt ctcagctggt
     3661 cgccggcgcg aacgaccgtc gaatggcaga aactgcagac cgcgaatggc gtggccgccg
     3721 agcggaactc gacgggcgcg ccacagttcg ggcaggctgc ccgataggcg cgttgaggcg
     3781 tgggttcggt ggccacggtg cgatgccgat gtgagaaggc gggacctcgc ggccccgcct
     3841 gctacatcgt cggcgacatc aggccagctt cttcaggagc tcggccttct tcgcgtcgaa
     3901 ctcttcctgc gtcaggatgc ccttggcctt gaaatcggca aggcgctcga gcgtggccat
     3961 cacgtcttcc ggcttgacgc cggcgctggc agcggccgca cccgcggctg ccgccggtgc
     4021 gccgggcgcc gctgtcgcca ggccctggcc gagctggttg gccattacct ggccgagagc
     4081 aaccccggca cccaggccga ccgcgctgcc gacgatgccg ccgccaccgc cgtcgccacc
     4141 tgctcccgcg gcgaacgcgg ggatcgattg cgccgtctgg ta
//
LOCUS       DASRWS010000078         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2622942, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000078 DASRWS010000000
VERSION     DASRWS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622942"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1664
                     /locus_tag="VFF72_01390"
     CDS             372..1664
                     /locus_tag="VFF72_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV91836.1"
                     /translation="MSSTFPARAAVSPSPEGTDGTRFRLLSALSFAHFLNDATQSTIL
                     AIYPLLKSNFGLSFTQIGLITLVYQITASLMQPVVGRYTDSHPMPYSLAFGMGSTLVG
                     LLVLAFAPTFSILLVAAVLVGTGSAIFHPEASRIARLASGGQHGLAQSIFQVGGNAGS
                     AAGPLIAAALVIPFGQHTVAWLAVGAIVGIFLLRRIGDWYSRQRRASARATPASTASP
                     LPRRAVRRAIAVLLVLLFSKYFYLASLTSYYTFYLMTRFHTTMESAQMHLFVFLFAVA
                     AGALLGGPIGDRIGRKRVIWGSILGVAPFTLALPHLDLAWTTACTVVIGFVLASAFSA
                     ILVFAQELVPGRVGTVSGLFFGFAFGMGGLGAAVLGAVADARGIEYVYAVCAYLPLLG
                     LLTALLPDMPSRPGRPQPPSTAADSPVAGGQVLGASAR"
     gene            1661..>2047
                     /locus_tag="VFF72_01395"
     CDS             1661..>2047
                     /locus_tag="VFF72_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HZV91837.1"
                     /translation="MIGPVPVEFVLFAIVLAGVALWHGHSLRVAVIGAVVIAAYKLAF
                     ASFAEGPGMPGLVAHLGHESVTLVNLLLLLLGFALLARTFEDSQVPAVLPRWLPDDWK
                     GAFVLLVLIFVLSSFLDNIAAAMIGGA"
BASE COUNT          240 a          748 c          682 g          377 t
ORIGIN      
        1 agcgcgtcgg cgaggaggcg gccggcgaca tcggtgctgc cgcccggcgg ataggcgacg
       61 acgatgtgga caggccggga aggccaggct tgcgccgcgg cgagcgtggg catcgcgaac
      121 gccagcgcga gagtcgtcag caaggtggca aggcggggca tctgttgtcc tggtgaaggt
      181 gtcgagcgcc ccggcaggcg ggaacgcgcg acgagacaat acaggacatc gtcggcgggc
      241 acggagatgc ggggcgccgg tcgacgcact ccgcgccccg atcggcggag ctttcacgca
      301 atcggtcttt cgactcgggt cgaagcggcg ccgcccggga atgccgggcc gatttcgcgt
      361 accgttgggg gatgtcaagc acctttcctg cgcgcgctgc cgtgtcgcca tcgccggagg
      421 gcaccgacgg cacgcgcttt cgcctgctgt cggcgctcag cttcgcgcac ttcctcaacg
      481 acgcgacgca gtcgacgatc ctggcgatct atccgctctt gaagagcaac ttcggcctca
      541 gcttcaccca gatcggcctc atcacgctcg tctaccagat caccgcgtcg ctgatgcagc
      601 ctgtggtcgg ccgctacacc gactcgcatc cgatgccgta ctcgctcgcc ttcggcatgg
      661 gctcgacgct ggtcggcctg ctcgtcctcg cgttcgcgcc gaccttttcg atcctgctcg
      721 tcgccgcggt gctcgtcggc acgggctcgg cgatcttcca ccccgaggca tcgcgcatcg
      781 cccgcctggc ctccggcgga cagcacggcc ttgcgcagtc gatcttccag gtgggcggca
      841 acgccggcag cgccgccggg ccgctgatcg cggcggcact ggtcatcccg ttcggccagc
      901 acaccgtcgc ctggctggcg gtcggcgcga tcgtcggcat ctttctcctg cgccggatcg
      961 gcgactggta ttcgaggcag cgtcgagcgt cggcgcgcgc gacgcccgca tccacggcca
     1021 gccccttgcc gcggcgcgcg gtgcggcgcg ccatcgcggt cctcctggtg ctgctctttt
     1081 ccaagtactt ctacctcgcc agcctgacga gctactacac cttctatctc atgacccgct
     1141 tccacacgac gatggaatcg gcgcagatgc acctcttcgt ctttctcttc gcggtggccg
     1201 cgggtgccct tctcggcggc ccgatcggcg accggatcgg ccgcaagcgc gtgatctggg
     1261 ggtcgatcct cggcgtcgcg ccgttcacgc tggcgctgcc gcacctcgac ctggcctgga
     1321 cgacggcatg cacggtcgtc atcggcttcg tcctggcctc ggcgttctcg gcgatcctcg
     1381 tcttcgcgca ggagctcgtg ccgggccgcg tcggcacggt ctcggggctg ttcttcggct
     1441 ttgcgttcgg catgggcgga ctcggcgccg ccgtgctcgg cgcggtcgcc gacgcgcgcg
     1501 gcatcgaata cgtctatgcc gtctgcgcct atctgccgct gctcgggctg ctcaccgcgt
     1561 tgcttcccga catgccctcg cggccgggcc ggccgcaacc gccctcgaca gccgccgaca
     1621 gcccggtcgc cggagggcag gtgctcggcg cgtcggcacg atgatcggcc cggtgccggt
     1681 cgagttcgtc ctcttcgcga tcgtcctcgc cggcgttgcg ctctggcacg ggcactcgct
     1741 tcgcgtcgcc gtcatcggcg ccgtcgtcat cgccgcctac aagctggcgt tcgcgtcgtt
     1801 cgccgaggga cccggcatgc ccgggctcgt cgcccatctc ggccacgagt cggtcacgct
     1861 ggtcaacctg ctgctgctgc tgctcggctt cgccttgctg gcgcggacct tcgaggacag
     1921 ccaggtaccc gccgtgctgc cgcgctggct gcccgacgac tggaagggcg ccttcgtcct
     1981 gctcgtcctc atcttcgtgc tttcgtcctt tctcgacaac atcgccgcgg cgatgatcgg
     2041 cggcgcg
//
LOCUS       DASRWS010000079         4245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3771957, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000079 DASRWS010000000
VERSION     DASRWS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771957"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2890)
                     /locus_tag="VFF72_01400"
     CDS             complement(<1..2890)
                     /locus_tag="VFF72_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZV91838.1"
                     /translation="MNFTDIFIKRPVLAMVVSLLIVVLGLRSLFGLPINQYPKTQNAV
                     VTISTTYYGADAQTVAGFITQPLEGAIAQAQGIDYLSSSSSSGVSVITATLRLNYDAN
                     RALTEISTQVNSVKNQLPPQAQTPVLTVQTGSTIDAMYLGFYSDSLPTNNVTDFLTRV
                     VKPKLDSIEGVQTAELLGARQFALRAWLDSNKLAAHGLTAADVSQALAANNYLAALGS
                     TKGQMVTVPLTAGTDLHTVEGFKRMAVRQTGDSIVRLEDVATVTLGSENYDFNVSFSG
                     VRSVFIGIKVAPEANILDVAKRVREAFPELRNQLPSGVTGEIVYDSTDYINTSIDEVV
                     KTLVEALVIVTVVIFLFLGSLRAVIVPVIAMPLSLVGTFFVMLALGYSINLLTLLALV
                     LAIGLVVDDAIIVVENVDRHMKEEGKTPMQAAFVAARELGSPIIAMTIVLIAVYVPIG
                     FQGGLTGALFTEFAFTLAGAVAVSGIVALTLSPMMASRFFKPSQDEGRFAKAIDRVFG
                     KVHRGYARALHSLLDTWPVLIVMAGILIILLAVMFKMSHSELAPEEDQGFIGALLVGP
                     PTATAQQMQTYAQQIFKIAEAEPEYTQMFQITGVPTVNQGIGGVILKDWGQRTRSAHE
                     VQQDLQQKWNKIAGARVAAFQFPSLPGASGLPVQFVITTTEPFDVLNSVAQQVVDKAR
                     ADNKFYFADVDLKLDKPQATVVVDRDKVAALGMTQQDVGQALGAALGGGYVNYFSIAG
                     RSYKVIPQVLQVDRLNPHDVLDFYIRTPNAGLIPASTVASIRHDIVPESINRFQQLNS
                     ATISGVSGASQGDILKYLTDTLHQVAPSGYNVDYSGQSRQYVQESGGFLITMGFAVII
                     VFLALGAQFNSFRDPIVILVSVPLALFGALIFIFLGFASMNIYTEVGLVTLMGLISKH
                     GILIVEVANKAQEAGKSKREAIEEAAGIRLRPILMTTAAMVFGVVPLVI"
     gene            complement(2975..4189)
                     /locus_tag="VFF72_01405"
     CDS             complement(2975..4189)
                     /locus_tag="VFF72_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV91839.1"
                     /translation="MKKTTKRMLWMIGGVVVLVALLALGFFLHIRKLIASAPKPAPQT
                     VSTTKVQALEWQPQLAAVGTLVAVRGVDVTSEIAGLVRSIEFKSGQDVRAGQVLVRLN
                     DDSDVAQLRSLQAAAELAATVVKRDKAQLEVQAISQAQVDADSADLKSRRALVAQQEA
                     TIAKKTIRAPFAGRLGITSVNPGQYVNPGDKIVTLQTIDPIHIDFNLPQGQLESLAVG
                     QVVDVVADGFSGVTFPGRITAINPKVDPSTRNVLIEATLPNSKRQLLPGMFANANVEV
                     GDKKRYLTLPQTAITYNPYGSTVFIVRTRREAEAAARAASGASSSAGPAAASAPAPAG
                     SAASPDSLVVQQVFVTTGETRGDQVAILKGLEEGQQVVTSGQVKLKNGTPVVVDNSVL
                     PADNPNPKPQEH"
BASE COUNT          685 a         1356 c         1464 g          740 t
ORIGIN      
        1 cgatcaccag cggaacgaca ccgaagacca tcgccgcggt cgtcatcagg atcggccgca
       61 gccggatgcc ggcggcttcc tcgatcgctt cgcgtttcga cttgccggcc tcctgcgcct
      121 tgttggccac ctcgacgatc aggatgccgt gcttgctgat gagacccatc agcgtcacca
      181 gcccgacctc ggtgtagatg ttcatcgagg cgaagccgag gaagatgaag atcagcgcgc
      241 cgaacagcgc gagcggcacc gacaccagga tcacgatcgg gtcgcggaag ctgttgaact
      301 gcgcgcccag ggcgaggaaa acgatgatga cggcgaagcc catcgtgatc aggaagccgc
      361 ccgactcctg cacgtactgg cgcgattgcc ccgagtagtc gacgttgtag ccgctgggcg
      421 ccacctggtg cagcgtgtcg gtgaggtact tgaggatgtc gccctgcgac gcgccggaca
      481 cgcccgagat cgtcgccgag ttcagctgct ggaagcggtt gatcgattcc ggaacgatgt
      541 cgtggcggat gctcgccacg gtgctcgccg gaatcagccc ggcgttgggc gtgcggatgt
      601 agaaatcgag cacgtcgtgg ggattgagcc ggtcgacctg cagcacctgc gggatcactt
      661 tgtacgagcg gccggcgatc gagaagtagt tgacgtagcc gccgccgagc gccgcgccga
      721 gggcctggcc gacgtcttgc tgggtcatgc cgagggcggc caccttgtcg cggtcgacga
      781 cgacggtggc ctgcggcttg tcgagcttca ggtcgacgtc ggcgaaatag aacttgttgt
      841 cggcgcgcgc cttgtcgacg acctgctgcg ccaccgagtt caggacgtcg aacggctcgg
      901 tcgtcgtgat cacgaactgc accggcagcc cggaagcgcc gggaagggac gggaactgga
      961 aggcggcgac ccgggcgccg gcgatcttgt tccacttctg ctgcagatcc tgctggactt
     1021 cgtgggcgct gcgggtgcgc tggccccagt ccttgaggat gacgccgccg atgccctggt
     1081 tcactgtcgg cacgccggtg atctggaaca tctgcgtgta ttcgggctcg gcctcggcga
     1141 tcttgaagat ctgctgcgcg taggtctgca tctgctgcgc ggtggcggtc ggcggcccga
     1201 ccagcagcgc gccgatgaag ccctggtctt cctccggcgc cagctcggaa tgcgacatct
     1261 tgaacatcac cgccagcagg atgatgagga tgccggccat cacgatcagt accggccagg
     1321 tatcgagcag gctgtgcagg gcgcgcgcgt agccgcggtg aaccttgccg aagacgcggt
     1381 cgatcgcctt cgcgaaccgg ccttcgtcct ggctcggctt gaagaatcgc gaagccatca
     1441 tcggcgacag cgtcagcgcg acgatgcccg aaacggcgac ggcgccggcg agcgtgaagg
     1501 cgaattcggt gaagagggcg ccggtgaggc cgccctggaa gccgatcgga acgtagacgg
     1561 cgatcagcac gatcgtcatc gcgatgatcg ggctgcccag ctcgcgcgcg gcgacgaagg
     1621 cggcctgcat cggcgtcttg ccttcctcct tcatgtgccg gtcgacgttt tcgacgacga
     1681 tgatcgcgtc gtcgacgacc aggccgatgg cgagcaccag cgcgagcagc gtcagcaggt
     1741 tgatcgagta gccgagcgcc agcatcacga agaacgtgcc gaccagcgac agcggcatgg
     1801 cgatcaccgg cacgatcacg gcgcgcaggc tgccgaggaa gaggaagatc acgaccgtga
     1861 cgatgacgag cgcctcgacg agcgtcttca cgacctcgtc gatcgaggtg ttgatgtagt
     1921 cggtcgagtc gtagacgatc tcgccggtca cgcccgaggg aagctggttg cgcagctccg
     1981 ggaaggcctc gcgaacccgc ttggcgacgt cgaggatgtt cgcctcgggc gccaccttga
     2041 tgccgatgaa gaccgagcgc acgccgctga acgagacgtt gaagtcgtag ttctcggagc
     2101 cgagcgtcac cgtggccacg tcttcgagcc ggacgatcga gtcgccggtc tggcggacgg
     2161 ccatccgctt gaacccctcg accgtatgca ggtcggtgcc ggcggtgagc ggcacggtca
     2221 ccatctggcc cttggtcgag ccgagcgcgg cgaggtagtt gttcgcggcc agcgcctggc
     2281 tgacgtcggc tgcggtcagc ccgtgggcgg cgagcttgtt cgagtcgagc caggcacgca
     2341 aagcgaactg gcgcgccccg agcagctccg cggtctgcac gccctcgatc gaatcgagct
     2401 tgggcttgac cacgcgcgtg aggaagtcgg tgacgttgtt cgtcggcagg ctgtcgctgt
     2461 agaagccgag gtacatcgcg tcgatcgtcg aacccgtctg caccgtcagc acgggcgtct
     2521 gggcctgcgg cggcagctgg ttcttgaccg aattcacctg cgtgctgatc tcggtcagcg
     2581 cccggttggc gtcgtagttg agccgcaatg tggccgtgat caccgacacg ccgctcgagc
     2641 tcgacgacga caggtagtcg atgccctggg cctgcgcgat cgcgccttcg agaggctgcg
     2701 tgatgaagcc ggcgacggtc tgcgcgtcgg cgccgtagta cgtcgtcgat atggtgacga
     2761 cggcgttctg cgtcttcggg tactggttga tcggcaggcc gaacagcgat cgcaatccca
     2821 gcacgacgat cagcaggctc accaccatgg cgaggaccgg ccgcttgatg aagatgtcgg
     2881 tgaagttcat gtcagtcttg ccgcgaagca acgggcgatc gccgaccggc ggccgggcga
     2941 acggaaaggg caggcagcct tctcatggcg ggcgtcaatg ttcctgcggc ttcggattcg
     3001 ggttgtcggc cggcagcacc gagttgtcga cgaccaccgg cgtgccgttc ttcagcttca
     3061 cctggccgct cgtcaccacc tgctggccct cctcgaggcc cttcaggatc gcgacctggt
     3121 cgccgcgcgt ctcgccggtc gtgacgaaga cctgctgcac gaccagcgag tccgggctcg
     3181 ccgccgatcc cgccggcgcc ggcgccgacg ctgccgcggg gccggccgac gacgacgcgc
     3241 cgctggccgc cctggcggcc gcttcggctt cgcgccgggt gcggacgatg aacaccgtcg
     3301 agccgtacgg gttgtaggtg atcgccgtct gcggcagcgt caggtagcgc ttcttgtcgc
     3361 cgacctcgac gttggcgttg gcgaacatcc ccggcagcag ctgtcgcttg gaattgggca
     3421 gcgtcgcctc gatcagcacg ttgcgcgtgc tcggatcgac cttgggattg atcgccgtga
     3481 tccggccggg aaacgtcacg ccgctgaacc cgtcggcgac cacgtcgacg acctggccga
     3541 cggcgaggct ctcgagctgg ccttgcggca ggttgaaatc gatgtggatc ggatcgatcg
     3601 tctgcagcgt gacgatcttg tcgccggggt tcacgtactg gcccgggttg acgctggtga
     3661 tgccgagccg ccctgcgaaa ggtgcccgga tcgtcttctt cgcgatcgtc gcttcctgct
     3721 gggcgacgag ggcacgccgg ctcttcagat cagccgagtc ggcgtcgacc tgggcctggc
     3781 tgatcgcctg cacctcgagc tgtgccttgt cgcgcttgac gacggtcgcg gcgagctcgg
     3841 cggcggcctg cagcgaacga agctgcgcga cgtcggaatc gtcgttgagg cgaaccagca
     3901 cctggccggc gcgcacgtcc tgtcccgact tgaattcgat gcttcgcacc aggcctgcga
     3961 tctcggaggt gacgtcgacg ccgcggactg cgaccagcgt gccgacggcg gcaagctgcg
     4021 gctgccactc gagcgcctgc accttcgtcg tcgacacggt ctgcggagcc ggcttgggcg
     4081 cggaggcgat cagcttcctg atgtgcagga aaaatccgag cgcgagcagg gcgaccagca
     4141 ccaccacgcc gccgatcatc cagagcatcc gcttggtcgt cttcttcatg aaccacctct
     4201 ctcctgttgg cgcgatcgcc gcggcggccg cttcttgtcg tctta
//
LOCUS       DASRWS010000080         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2911910, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000080 DASRWS010000000
VERSION     DASRWS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911910"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFF72_01410"
     CDS             complement(<1..234)
                     /locus_tag="VFF72_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZV91840.1"
                     /translation="MEPTPRPRRRTASFVYLASQSPRRAELLRQIGVRHELLLADAGE
                     DVEALEAVRAGELPIDYVRRVTLAKLAAARRRRA"
     gene            complement(235..702)
                     /gene="rlmH"
                     /locus_tag="VFF72_01415"
     CDS             complement(235..702)
                     /gene="rlmH"
                     /locus_tag="VFF72_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZV91841.1"
                     /translation="MKLLLVGVGQRLPAWVGTVFDDYARRFPPELRLELKAVKAEPRD
                     SRTPAQLMAAEAKRIDAALPRGVRRIALDEQGERVTSRGLADRLRDWQRDGGDVGLLI
                     GGPDGLDPALKAGCAERLRLSDLTLPHAFVRILVAEALYRAWSIDAGHPYHRE"
     gene            complement(740..1438)
                     /gene="rsfS"
                     /locus_tag="VFF72_01420"
     CDS             complement(740..1438)
                     /gene="rsfS"
                     /locus_tag="VFF72_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZV91842.1"
                     /translation="MDIRKLQRVIVDGLEDVKAQDIVVFNTEHLSPLFERVIVASGTS
                     NRQTKSLAASVREAVKAAGAPPPRVEGEGNGEWIIVDCGAAVVHVMQPAIREYYHLEE
                     LWGDKPVKMKIASAAPRLALASTSAAAEPGGDASARRKSARAPRQSMARLSPPATASA
                     RKGSAAPSSGATSKRSVADGPSAARKSPARKTPARPRVQRTTGTVTSARKTPATPSSS
                     SPRRPAKRATKSTT"
     gene            complement(1439..2182)
                     /gene="nadD"
                     /locus_tag="VFF72_01425"
     CDS             complement(1439..2182)
                     /gene="nadD"
                     /locus_tag="VFF72_01425"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZV91843.1"
                     /translation="MGRRDRRQQRSAARAATCRAEVAGAGFDIDIGGAGGGPGAGLGL
                     GESVMRVGLFGGSFDPVHNAHVALARTALEQLGLDELRWIPVGRPWQKARRLASGADR
                     EAMVRLAIAGEPRFVLDRSELRRRGVSFTLDTVRELAAARPDDERILVIGQDQYASLH
                     TWRDWKELLGAVTLAVANRPGAGLAINPQIAAVAHRPVMLPMMDISSTEIRRRVAAGE
                     PIAGLVPPAVASYIEQHRLYRDGGDPTRS"
     gene            complement(2169..>2854)
                     /gene="hemF"
                     /locus_tag="VFF72_01430"
     CDS             complement(2169..>2854)
                     /gene="hemF"
                     /locus_tag="VFF72_01430"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HZV91844.1"
                     /translation="GAALADAPFEAMGVSLVFHPRNPYVPIVHMNVRMLAATPSTGKP
                     ATWFGGGMDLTPCYGFEEDAVHFHVVNRDALRPFGADKYPRFKRWCDDYFFLRHRSEP
                     RGIGGIFFDDFDEGGFDNGFALIAAVGDAFIDAYLPILRRRRDTPHGERERAFQTYRR
                     GRYVEFNLVFDRGTLFGLQSGGRTESILMSMPPLVTWRYDWHPEPGTPEARLYSDFLR
                     ARDWVDGPP"
BASE COUNT          399 a         1012 c         1032 g          411 t
ORIGIN      
        1 cgcgcgcctg cgccttgccg cggcgagctt ggccagcgtc acacggcgca cgtagtcgat
       61 cggcagttcg ccagcgcgca ccgcctcgag cgcctcgaca tcctcgccgg cgtcggcaag
      121 gaggagctcg tggcgaacgc cgatctgccg cagcagctcg gcccggcgcg gactttgcga
      181 cgccaggtag acgaaggacg ccgtccttcg acgcggacgc ggagtcggtt ccatctattc
      241 gcggtggtac gggtggcccg cgtcgatcga ccaggcccgg tacagcgcct cggcgacgag
      301 gatgcgcacg aaagcgtgcg gcagcgtcag gtccgacagg cgaaggcgct cggcgcaacc
      361 cgccttcagc gccggatcga gcccgtccgg accgccgatc agcagcccga cgtcgccgcc
      421 gtcgcgttgc cagtcgcgca gccggtcggc gaggccgcgg ctggtgacgc gctcgccctg
      481 ctcgtcgaga gcgatccggc gcacgccgcg cggcagcgcg gcgtcgatgc gcttcgcctc
      541 cgccgccatc agctgcgcgg gcgttcgcga atcgcgcggc tccgccttca ccgccttcag
      601 ctcgaggcga agctcgggcg gaaagcgccg tgcgtagtcg tcgaagacgg tgccgaccca
      661 cgccggcagg cgctggccga cgccgacgag cagcagcttc accgtacggg ctcgccgccg
      721 gtcgaaagcg ccttcggcgt caggtcgtcg atttcgtcgc ccgcttggcc ggtcgacgcg
      781 gcgacgacga cgacggtgtc gcgggcgtct ttcgcgccga cgtcaccgtg cccgtcgtgc
      841 gctggacgcg cggccgggcc ggcgtcttgc gcgccggaga tttcctggcg gccgatggac
      901 cgtccgccac gctgcgcttc gacgtggcgc ccgatgaagg cgcggccgag cccttgcgcg
      961 ccgatgcggt ggccggaggc gataaacgcg ccatcgactg gcgcggcgca cgcgcgctct
     1021 tgcggcgtgc cgacgcgtcg ccgcccggct cggccgcggc ggacgtggac gcgagggcca
     1081 gccgcggcgc cgccgaggcg atcttcatct tgacgggctt gtcgccccac agctcctcga
     1141 ggtggtagta ctcgcggatg gccggctgca tcacgtggac gacggcggca ccgcagtcga
     1201 cgatgatcca ttcgccgttg ccttcgccct cgacgcgcgg cggcggcgcg ccggccgcct
     1261 tcaccgcctc gcggacgctc gctgcgagcg acttcgtctg ccggttgctc gtgcccgagg
     1321 cgacgatgac gcgctcgaag agcggcgaca ggtgctcggt gttgaaaacc acgatgtcct
     1381 gggccttgac gtcttccagt ccatcgacga tgacgcgctg cagcttgcga atgtccattc
     1441 agctcctggt cgggtcgccg ccatcacggt acaggcggtg ttgctcaata tagcttgcga
     1501 cggccggcgg gacgagcccg gcaatcggct cgcccgcggc gacgcgccgc cggatctcgg
     1561 tcgacgagat gtccatcatc ggcagcatca ccggccggtg tgccaccgcc gcgatctgcg
     1621 ggttgatcgc cagtccggcg ccgggccggt tggcgactgc cagggtcacc gcgccgagca
     1681 gctccttcca gtcgcgccag gtgtgcaggc tcgcgtactg gtcctggccg atgacgagga
     1741 tgcgctcgtc gtcgggccgc gcggcggcca gctcacggac cgtgtcgagc gtgaagctga
     1801 cgccgcgccg gcgcagctcg cttcggtcga ggacgaagcg gggctcgccg gcaatcgcca
     1861 gccgcaccat cgcttcacgg tcggcgccac tggccaggcg gcgcgccttc tgccagggcc
     1921 ggccgaccgg gatccagcgg agctcgtcga ggccgagctg ttcgagcgcc gttcgcgcca
     1981 gcgcgacatg cgcgttgtgc accggatcga aactgccccc gaacaggccg acgcgcatca
     2041 cggattcgcc gaggccgagg ccggcaccgg gcccgccgcc cgcaccgccg atatcgatgt
     2101 cgaagccggc gccggcgact tcggctcgac aggtcgcggc ccgtgccgcg ctgcgttgct
     2161 gccgccgatc acggcggccc atcgacccag tcgcgggcgc gcaggaagtc gctgtagagc
     2221 cgcgcctcgg gcgtgccggg ctcggggtgc cagtcgtagc gccaggtgac gagcggaggc
     2281 atcgacatca ggatgctttc ggtgcggcca cccgactgca ggccgaacag ggtgccgcgg
     2341 tcgaagacga ggttgaattc gacgtagcgg ccgcgccggt aggtctggaa ggcgcgctcg
     2401 cgttcgccgt gcggcgtgtc gcggcgacgc ctgaggatcg gcaggtaggc gtcgatgaag
     2461 gcgtcgccga ccgccgcgat cagcgcgaag ccgttgtcga atccgccttc gtcgaaatcg
     2521 tcgaagaaga tgccgccgat gccgcgcggc tcgctccggt gcctgaggaa gaaatagtcg
     2581 tcgcaccagc gcttgaagcg cgggtacttg tcggcgccga agggccgcag cgcgtcgcga
     2641 ttgacgacgt ggaaatgcac cgcgtcttcc tcgaacccgt agcagggagt gaggtccatg
     2701 ccgccgccga accacgtcgc cggcttgccg gtcgaaggcg tcgccgcgag catccgcacg
     2761 ttcatgtgca cgatcggcac gtaggggttg cggggatgga agacgagcga gacacccatc
     2821 gcctcgaacg gcgcgtcggc cagcgccgcg cccc
//
LOCUS       DASRWS010000081         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_611680, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000081 DASRWS010000000
VERSION     DASRWS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611680"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /gene="modA"
                     /locus_tag="VFF72_01435"
     CDS             complement(<1..364)
                     /gene="modA"
                     /locus_tag="VFF72_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV91845.1"
                     /translation="MSLRPIRRLRPVASLVALALLLLGNAASAADVTVSAAASLTNAF
                     KEVGAAFEQAHPGVEVQLNFAASGVLLQQIARGAPVDVFASADQQTMNQAGQRHLVMA
                     ATRVDFASNSLVVIVPADA"
     gene            614..922
                     /locus_tag="VFF72_01440"
     CDS             614..922
                     /locus_tag="VFF72_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91846.1"
                     /translation="MGASIADPGKESLRIEAIDGDAEMNEDDEDLTAELAEAAEQAAA
                     SVSRLTAVLRRIELANRLRQLQRIEELETVQAALGVRTRESKMAGALIARARGGARPG
                     "
     gene            complement(1045..1416)
                     /locus_tag="VFF72_01445"
     CDS             complement(1045..1416)
                     /locus_tag="VFF72_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV91847.1"
                     /translation="MDHEVRVLVADDAEDVADVVAHALTLDGYSVRIAPDGEQAKAMV
                     AEFAPHCVLFDVDMPHLDGNQLSTWLREQYGDDIVLIAMTGWSDTDRRVADTFVRVDH
                     YLRKPIDLDTLRRVLPPVNDT"
     gene            1779..2465
                     /locus_tag="VFF72_01450"
     CDS             1779..2465
                     /locus_tag="VFF72_01450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZV91848.1"
                     /translation="MASSTITLTAADGFASQAYVSEPSRPPKGAIVVLQEIFGVNSHI
                     RAVADGYAAAGYLAIAPATFDRVERGVELGYSADDVTRGARLKAAVEALPAPGVLQDI
                     QAAVSHVAGAGKVGIVGYCWGGLLVWRSANKVEGLSAAVAYYGGGMTVGNEASRKPAV
                     PTMAHFGDQDAHITVDSVKAFEQAHPEVEVHLYAANHGFNCDQRGSYNASAAATALER
                     SLYHFGKHVG"
     gene            2714..3448
                     /locus_tag="VFF72_01455"
     CDS             2714..3448
                     /locus_tag="VFF72_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZV91849.1"
                     /translation="MTAVLFSMATFASTLIGGLFALGFRDRLHYLLSFTAGVLLGVVS
                     FEVLPEVFELAHAHSLDTMRPMVALVVGFLFFHSLEKFVLIHHAHEEDYAQHRHPQVG
                     VLSATALVGHSLLDGVAIGLAFQASQVVGITVAVAVIAHDFCDGLNTVGLMLAHHNTT
                     RHAKVMLVLDAVAPMLGAASTLLFRLPAAGLVLYLGFFAGFLLYIGVSDILPEAHSGA
                     PPDTAVRLIGLTALGAAFIFVVARFA"
     gene            complement(3487..>4432)
                     /locus_tag="VFF72_01460"
     CDS             complement(3487..>4432)
                     /locus_tag="VFF72_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV91850.1"
                     /translation="LEEVDGGAIYIGDRDVTDDPPKKRDIAMVFQNYALYPYLTVAAN
                     IGFPLKIAGVKKDVRERKVLEAAKMLGLTEYLERKPGQLSGGQRQRVAMGRAIVRQPQ
                     VFLFDEPLSNLDAQLRVQMRFEIQKLHTRVPTTSIYVTHDQVEAMTLADRVMVMNAGR
                     VEQIGPPMTIYSRPESLFVAGFIGSPSMNRVKGRVDSTGGAIAFEDGSTFRLPGAPIA
                     AWQGRTLVVGMRPEHLERDDSAVPGLSLRAEVVEPLGADTLVHGTLGGGLFIVRLPGG
                     DPVAANETVPLRIARDRLHFFDADSGRRIEPGEPATRV"
BASE COUNT          701 a         1497 c         1505 g          729 t
ORIGIN      
        1 ccgcatcggc gggaacgatg acgaccagcg agttgctggc gaaatcgaca cgcgtggccg
       61 ccatcacgag gtgccgctgc ccggcctggt tcatcgtctg ctggtcggca cttgcgaaga
      121 catcgaccgg tgcgcccctg gcgatctgct gcagcagcac gccggaggcg gcgaaattga
      181 gctggacctc gacgcccgga tgcgcctgct cgaatgcggc gccgacctcc ttgaaggcat
      241 tcgtcaggct ggcggcggcg gagacggtga cgtcggcggc agacgccgcg ttgccgagca
      301 gcaggagcgc caaggcgacg agagaagcga ccgggcgcag acggcgaatc gggcgaagag
      361 acatgaggcg tcggtcgacg ggttggaaat gcacggccga ctataacggt acccgaccac
      421 gacatcgccc ctgagggacc gggacgaatc gagcttcgcc tgtcgcgtcg accggggatc
      481 atccctcgtc gaacaccttc cacgtctcgg gctgcggccg ccggaccgaa tcgcaccgtc
      541 ggcgcacgcc tgccgcggac cccgtgcgac gccgctgtag gaaaaacgct cgcctgcgat
      601 ggacagacgg cgcatgggcg cttcgattgc cgatccgggg aaggagtcgc ttcgcatcga
      661 ggcgatcgat ggagatgccg agatgaacga ggatgacgaa gacctgacag ccgaactggc
      721 cgaagccgcc gaacaggccg cagcttcggt gagccggctc accgccgttt tgcgacgcat
      781 cgagttggcc aaccggctgc gacagttgca gcggatcgag gagctcgaga ccgtgcaggc
      841 ggccctgggc gtgcgcacgc gcgagagcaa gatggccggc gcgctgatag cccgggctcg
      901 cggcggcgca cgaccgggat gaatgtcgaa gccgaccttg ccaggaggcc ctggaatcag
      961 cgtacgcgcg tcgtcgcgtc gttggcgacg gcgtggcctg cgatcggctg gtcgcctcgt
     1021 cgcccgcgat gaccgtgcct gcgatcaggt gtcgttgaca ggcggcagga cgcgacgcag
     1081 cgtgtcgagg tcgatgggct tgcgaaggta gtggtcgacg cgcacgaagg tgtcggcgac
     1141 gcgacgatcc gtatcgctcc agccggtcat ggcgatgagg acgatgtcgt cgccgtactg
     1201 ctcgcgcagc cacgtcgaca actgattgcc gtcgaggtgc ggcatgtcga cgtcgaacag
     1261 cacgcagtgc ggcgcgaatt cggccaccat ggccttcgcc tgctcgccgt cgggagcgat
     1321 gcggaccgaa tagccgtcga gcgtcagcgc gtgcgcgacg acgtcggcga cgtcctcggc
     1381 gtcgtcggcc accagcaccc ggacttcgtg atccactgca gcgatctccc cttcgatccc
     1441 gacgtatttt gcacggacgg cccgggccgt tcgtccgcgg aacaaaatca gtgcgcgctg
     1501 cgcgagtgcc gttttgtccc gtcgaggccc gccgggtgcg aagcccccct gaggcgcttc
     1561 atatccgcgg gatgatggca cgcgggccgg aaaagcaccg ccccgctcgg cccggcccgg
     1621 cccggcccgg cccggcccgg cccggcccgg cccggcccgg caagatgctc gccgaaacga
     1681 tcgcgcaaga ccattcggac aggcaacggc ctcgtgcatg aaaatcgtgg agcgccgaac
     1741 ccgaacccag cgctgccctt tttcaggaga caaattccat ggcctcatcc acgatcaccc
     1801 tcaccgccgc cgatggcttc gcgtcgcagg cctacgtatc cgagccgtcg cgtccgccga
     1861 agggggcgat cgtcgtgctg caggagatct tcggtgtgaa ttcgcatatc cgcgccgtcg
     1921 ccgacggcta tgcggctgcc ggctatctcg ccatcgcgcc tgcgacgttc gaccgcgtcg
     1981 agcgcggcgt cgagctcggc tattcggccg acgacgtgac gcggggggcg cgcctgaagg
     2041 cagcggtcga ggcgcttccg gcgccaggcg tgctgcagga cattcaggcc gcggtctcgc
     2101 atgtcgcggg cgcgggcaag gtcggcatcg tcggctactg ctggggcggc ctgctggtct
     2161 ggcgttcggc gaacaaggtc gagggcctgt ccgctgcggt cgcctactac ggcggcggga
     2221 tgacggtggg caacgaagca tcgcgcaagc ccgctgttcc gaccatggcg catttcggcg
     2281 accaggacgc ccacatcacg gtcgacagcg tgaaggcctt cgagcaggcg caccctgaag
     2341 tcgaagtgca cctctacgcc gcgaatcacg gcttcaactg cgatcagcgc ggctcctaca
     2401 acgcctcggc tgcggcgacc gcgctcgaga ggtcgctcta tcacttcggc aagcacgtcg
     2461 gatagacgag accgaccggc aagccggccg ccccggcgcg gcgcgaagct ccgggcgcgc
     2521 tgtcgccacg ctgatcgggc atcggcgatg tgaggctttc tgtcggaccc tcttgcccgg
     2581 cgtgcagcag gtggcgcatc ggcagtatcg agcgacacgg aaggactcgc gtgcgacctg
     2641 gtcccggaca ggatcgaacg tccggtcgga atggatcccg gcctgatctg ccgggcgttg
     2701 atacttcatc cccatgaccg cagtcctgtt ttccatggcg accttcgcct cgaccctgat
     2761 cggcgggttg ttcgcgcttg gattccggga caggctgcac tacctcctca gcttcaccgc
     2821 aggggtgctg ctgggcgtcg tcagtttcga ggtccttccc gaggtcttcg agctcgcgca
     2881 cgcgcattca ctggacacca tgaggccgat ggttgcgctc gtcgtcggct ttctgttctt
     2941 tcacagtctc gagaaattcg tgctgatcca ccacgcgcac gaggaagact acgcgcagca
     3001 tcgtcacccc caggtcggcg tgctatcggc gacggcactc gtggggcaca gcctgctcga
     3061 cggcgtcgcg atcggactgg cgttccaagc ctctcaagtc gtcggcatca ccgtcgccgt
     3121 ggcggtgata gcgcacgact tttgcgacgg cctcaacacg gtgggactga tgctggccca
     3181 tcacaacacg acgcgccatg cgaaggtgat gctcgtcctg gacgccgtgg cgccgatgct
     3241 cggggcggcg tcgactctgc tcttccggct tcctgcggct ggcctggttc tctacctggg
     3301 cttcttcgcc ggattccttc tgtatatcgg cgtctcggat atcctgcccg aggcccattc
     3361 aggagctcct cccgacacgg ccgtcaggct catcggactg accgccctcg gcgccgcgtt
     3421 catcttcgtc gtagcccgct tcgcctgaac atcggcggcg gttgcgcttg caccggcgcg
     3481 tccgcctcag acgcgggtcg ccggctcgcc aggctcgatc cggcgaccgc tgtcggcgtc
     3541 gaagaagtgc agtcgatcgc gcgcgatgcg caacggcacg gtctcgttgg ccgcgaccgg
     3601 atcgccgccg ggcaggcgca cgatgaagag tccgccgccg agcgtgccgt gaacgagggt
     3661 atcggcgccg agcggctcga ccacctcggc gcgcaggctc aggccgggca cggcgctgtc
     3721 gtcacgctcg agatgctcgg gccgcatgcc gacgacaagg gtccggccct gccaggcggc
     3781 gatcggcgca cccggcagcc tgaacgtgct gccatcctcg aaggcgatgg cgccgccggt
     3841 cgaatcgacc ctgcccttga cccggttcat cgacggcgag ccgatgaagc cggcgacgaa
     3901 caggctctcg gggcgcgaat agatcgtcat cggcgggccg atctgctcga cccgcccggc
     3961 gttcatcacc atcacccgat cggccagggt catggcctcg acctggtcgt gcgtcacgta
     4021 gatgctggtg gtgggaacgc gggtgtgcag cttctggatc tcgaagcgca tctgcacccg
     4081 cagctgcgca tcgagattgg agagcggttc gtcgaacagg aacacctgcg gctggcgcac
     4141 gatcgcccgc cccatcgcga cccgctggcg ctggccgccc gacagctggc cgggcttgcg
     4201 ctccaggtac tcggtcagcc cgagcatctt cgccgcctcc agcaccttgc gctcgcgcac
     4261 gtccttcttg acccccgcga tcttgagcgg gaatccgatg ttggcggcga cggtgaggta
     4321 cgggtagagc gcgtagttct ggaagaccat cgcgatgtcg cgcttcttgg gcggatcgtc
     4381 ggtcacgtcg cggtcgccga tgtagatcgc gccgccgtcg acctcctcga gg
//
LOCUS       DASRWS010000082         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1760897, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000082 DASRWS010000000
VERSION     DASRWS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760897"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..897)
                     /locus_tag="VFF72_01465"
     CDS             complement(289..897)
                     /locus_tag="VFF72_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZV91851.1"
                     /translation="MLQRSATGDRDAFAAMYTLTSGKVFGVVLRIMRDQAEAEEVLQD
                     VYVSVWQQARTYDRTKANAMTWLVSIARNRAIDSLRRRKARPLTQSACADDNTTEDYA
                     DAVAGTLGPAETLNQRQEAATLGRCLTMLAPHERTSVLLAFYDGLSHREIAERMRRPV
                     GTVKSWIKRSLASLKCSVEAAAEQGASCRRELRESEDAASRV"
     gene            1178..2002
                     /locus_tag="VFF72_01470"
     CDS             1178..2002
                     /locus_tag="VFF72_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZV91852.1"
                     /translation="MDELSDLLERFPLRAGVFYTGAMCGTYDFPRDVKPGHFHLVRSG
                     RLELLGDGHDPQGIDRPSVVFMPDGGTHRFVADPGVDILCATVRFGSGDAGPVVRALP
                     SLVVLPLAEAPPLMALCDAMFEEAGSNRPGRQATLNRLCELGVIAALRSCIDSERISS
                     GLLAGLADPRLSRAMVAMHEQPAREWSLAELAALATMSRARFAAQFRTVVGQTPGDHL
                     AARRVQEAQRLLRSGLSLKQVADRVGYGSASALTRAFTRVVGVAPSHWRVGPRPVP"
     gene            complement(2116..>2912)
                     /locus_tag="VFF72_01475"
     CDS             complement(2116..>2912)
                     /locus_tag="VFF72_01475"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZV91853.1"
                     /translation="YERYSWQGVGLTLILDLFLFGAIGAAVWAVQMLWIPVTAAGIVN
                     GIGHWWGYRSFETADASRNFMPWGVLIGGEELHNNHHTYPTSAKLSVKPYEFDIGWAY
                     IRILEALRLASVHRVAPRLRLGSAQATPDGKTLEAISTHRHEVMARYARRVRSAAGVE
                     LRRMRVDRADANRVAAMKLARRWLHRDAERIPVGAKRQIAQALVISPHLAQLVRMRES
                     LRHLWTGTNVSAEQLVEELRVWLQEAENSGLATLQEFAGRLRLLEA"
BASE COUNT          422 a          991 c          970 g          529 t
ORIGIN      
        1 tagaagcctt cctgcaccag gaggtcgacg tggccgagat cgacgactcc gacattgacg
       61 gtcatcttct cggtctcacc gatgcgtggc cgcggctctg cggcatcgac ccttcgtttc
      121 atgacatcca cctggaatcc atgcagatgt cgattggaac atgttccgcg caccggcgta
      181 cggagggggc ggcgttggcg tcatcaccgg ccttcggctc cggccgcctg catccggccg
      241 cgtgcacgct tgtgcgcgat accgcacata cccggcggcc ctggcgattc atactcgcga
      301 cgcggcgtcc tcgctctcgc gcagttcccg gcgacacgaa gcgccctgtt cggccgccgc
      361 ctcgacagag catttgagcg acgcgagcga ccttttgatc cacgacttga cggttcccac
      421 cgggcgtcgc atgcgctcgg caatttccct gtgactgagt ccgtcgtaga acgcgagcaa
      481 gacgctggtt cgctcatggg gtgcgagcat ggtgaggcac cggccgagcg tcgccgcctc
      541 ctgtcgttgg ttcagcgtct cggctggccc gagtgtcccg gcgaccgcat ccgcgtagtc
      601 ctcggtcgtg ttgtcgtcgg cgcaggccga ttgcgtcagt ggccgcgcct tccttctccg
      661 gaggctgtcg atggcccggt tgcgggcgat gctgacgagc caggtcatcg cattggcctt
      721 cgtgcggtcg taggtccgtg cttgctgcca gacgctcacg tacacgtcct gcagcacctc
      781 ctcggcctcc gcctggtcgc gcatgatccg cagaacgacg ccaaacacct tgccgctcgt
      841 gagcgtgtac atggcggcga aggcgtcgcg atcgcccgtt gcacttcgct gcagcaagag
      901 cgctccctcg tcgtctggct ttgcggtcgg atccgcgtgc gacgggtgcc gagacccttg
      961 cttcgaccat ggccgtcgct gcgggatgtc gaagtgccgt gcggacgcgt ctacggaaag
     1021 cgctgccggc tcgggcagtg acatggggaa cccttttgaa acgctgcgga cgacagtcgc
     1081 gtcggcaacc accggacctc gggcgctcga gcgagacgat gggtcatgaa ttcaagacgg
     1141 gcagccatgc gccctttcat ccgtgcgagc cgtgaagatg gatgagctgt ccgatctgct
     1201 cgagcgcttt ccgctgcggg cgggagtctt ctatacgggc gccatgtgcg gcacgtatga
     1261 cttcccgcgc gacgtcaagc ccggccactt ccatctcgtg aggtccggtc ggctcgagct
     1321 gctcggcgat ggtcatgatc cgcaagggat cgacaggccc agcgtggtct tcatgccgga
     1381 tgggggcacc caccgcttcg tggccgatcc cggcgtcgac atcctgtgcg caaccgtccg
     1441 gttcggaagc ggcgacgccg gccccgtcgt ccgtgcgctg ccgtcgctcg tggtgctgcc
     1501 gctcgccgaa gcgcctccct tgatggcctt gtgcgacgcg atgttcgagg aggccggctc
     1561 gaatcgaccc ggtcggcagg cgacattgaa tcggctgtgc gagctcggcg tcatcgccgc
     1621 gcttcgatct tgcatcgaca gcgagcgcat cagcagcggg ctgctcgccg gcctggccga
     1681 tccgcgattg tcccgggcga tggtcgcgat gcacgagcag cccgcgcggg agtggtcgct
     1741 tgccgaactg gcggcactcg cgacgatgtc gcgcgcccgg ttcgcggctc aattccggac
     1801 ggtggtcggg caaacgcccg gcgaccacct cgccgctcgg cgggtccagg aagcgcagcg
     1861 gttgcttcgc agcggcctgt ccctcaagca agtcgccgat cgggtcggat atggcagcgc
     1921 cagcgccttg acgcgcgctt tcacccgcgt ggtcggcgtg gcgccgtcgc actggcgtgt
     1981 cgggccgcgg cccgtgccct gagacggcga cggcgaggac tgcgctcgct cgcgctcgcc
     2041 cgctcgtcaa gctcgggtcc gggtgcggtg tcgcgtcgtg cctggctcgg tgtcgcctcc
     2101 cgcaggacgc gtcgctcacg cttcgagcaa gcgcaggcga ccagcgaatt cctgcagtgt
     2161 cgcaaggccg ctgttttccg cctcctgaag ccagacgcgc aattcctcga cgagctgctc
     2221 ggccgacacg ttcgtgcccg tccagagatg gcgcaagctt tcgcgcatcc gcaccagctg
     2281 cgcgagatgc ggactgatga cgagtgcctg cgcgatctgc cgcttcgcgc ccaccgggat
     2341 cctctccgcg tcacggtgca gccagcgccg ggcgagcttc atcgccgcca ctcgattcgc
     2401 atccgcgcgg tcgacgcgca tcctgcgcag ctcgacgccg gccgccgacc tgacgcgccg
     2461 cgcatacctc gccatcactt catggcgatg ggtggagatc gcttcgagcg tcttgccgtc
     2521 cggcgtcgcc tgcgccgagc ccagacgcag gcggggcgcg acccggtgga cgctggccag
     2581 tcgaagcgcc tcgaggatgc ggatgtacgc ccagccgata tcgaattcat acggcttgac
     2641 ggagagcttc gccgaggtcg gataagtgtg atggttgttg tgcagctcct cgccgccgat
     2701 gaggactccc cacggcatga aattgcggct cgcgtcggcc gtctcgaagc ttcgatagcc
     2761 ccaccagtga ccgatgccgt tgacgatgcc tgcggcggtg accggaatcc agagcatctg
     2821 cacggcccag acggcggcgc cgatggcgcc gaacaggaag agatcgagga tgagggtcag
     2881 gccgacaccc tgccagctgt accgttcgta ga
//
LOCUS       DASRWS010000083         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1473166, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000083 DASRWS010000000
VERSION     DASRWS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473166"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFF72_01480"
     CDS             complement(<1..330)
                     /locus_tag="VFF72_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZV91854.1"
                     /translation="MTMPLMKPRKKNPVLRTRQMNLPPWARGRTALGMTAAAAEGRFE
                     LQVCQQCGAVQYPPRDACHRCLSSALRWTPQTGEGELLSETVLHHSNDPFYRERLPWR
                     LGLVRLDA"
     gene            complement(395..1561)
                     /locus_tag="VFF72_01485"
     CDS             complement(395..1561)
                     /locus_tag="VFF72_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZV91855.1"
                     /translation="MPRQSYEGVAVAVPVTVPYVRYSTQGAHWFIGQAMQALVRESGI
                     AKEQIDGLAISSFSLGPDTAVGVTQHLGLSPRWLDHIPTGGASGVMALRRAARAVQAG
                     DADVVACIGADTNHVDSFRQMLGGFSHFARDASYPYGSGGPNSIFAFITAHYMRAYGA
                     TREDFGRICVAQRTNALKNPNALFKTPLSIDAYMAARPIAEPIHLFDCVMPCAGADAF
                     LVMSEERARDLGLGHVLLRGAIERHNAYADDPILIRGGWRVDRDDLFAQAGVRPDAID
                     FVQSYDDYPVVVMLHFEGLGFCGEGEGAEFVRSRTLTHDGTFPNNTGGGQLSTGQAGC
                     AGGFLGMTEAIRQLTGQAGERAVADAELGLVGGFGMVTYDRCLCTGAVILGRAS"
     gene            complement(1588..3504)
                     /locus_tag="VFF72_01490"
     CDS             complement(1588..3504)
                     /locus_tag="VFF72_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV91856.1"
                     /translation="MTSNDPNRAIAPPDSTTVHGAFARVAARRGDADFLCIEPETART
                     YGIDAGARSWREVAVEVERLRAAYAQAGYGHGHRVGLLLENRPAFFTHWLALNALGIS
                     IVPIHADMRAAEWTYLIGHSEMSLAVVQPGREGALRAAANEAGAQLETIRPEALGRIP
                     AARSPARQAGHAIGRTSECALLYTSGTTGRPKGCRLANGYFLRAGEWYLGLGELAAVR
                     ADVERIITPLPLSHMNALAFSSMAAILSGGAIVQLDRFHPATWWQSVHDSRATIVHYL
                     GVMPAMLLGLPPGPHDRDHAVRFGFGAGVDPRHHAAFEERFGFPLLEAWAMTETGAAA
                     CIMAHREPRHVGTRCFGRAEPFVETRVVDHHGADVADGASGELLVRAAGAESRNDFFL
                     GYLKDEPATEEAWAGGWFHTGDVVQRTAEGDFHFVDRRKNVIRRSGENISAVEVESVL
                     DMHPAVAASAVAATPDPVRGDEVLACVVLRKAPNLPALEQIAASIVRYAMERLAYFKA
                     PGYVAFVDALPVTVSQKIQRGELKDLAKTLPGKPNCIDTRQMKRRRKKELQAAATAAG
                     ELETGSSKLDSGSGRLQTGSGKLQTGSGKLQTGNGKPETGGEKPEASGDKGDAGDTLD
                     LGGYKLDISGDKLY"
     gene            complement(3501..3998)
                     /locus_tag="VFF72_01495"
     CDS             complement(3501..3998)
                     /locus_tag="VFF72_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZV91857.1"
                     /translation="MSAVPHHDVSERRLIDFVVHEARLLDEKRYDEWNALFTDDAIYW
                     VPLVPGQPEGLDHTSHLYEDKLLRDLRIERLKSPRAFSQQPPTRSLHLLQTPTVEPAA
                     GAGEHLVRTVFQYTESQGDEMNTFVGVCRHRLVVDAQGALRILQKRVDLINSDAALPA
                     VQLFI"
     gene            complement(4081..>5256)
                     /locus_tag="VFF72_01500"
     CDS             complement(4081..>5256)
                     /locus_tag="VFF72_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZV91858.1"
                     /translation="GHASQLPQPGDYITNEIAGRPLIVVRHQDGSVRVLMNRCAHKGS
                     RLVSAPCGNTGKLFRCPYHAWTYRTDGSLLSMPLKNGYEGTALHECEAGHGLATVKNV
                     ALYRGFIFVKLNDVGPAFEEYFGESLSSIDNLADRSPEGRLEIAGGCLRFMHQCNWKQ
                     YVENLNDTMHPMVVHESSAGTAKRMWLGQPADAPKPMAIEQFVPFMSDYKFFEDMGVR
                     VFDNGHGFSGANFSIHSKYSAIPAYEKAMKEAYGEERTAEILGLVRHNTVYYPNLTIK
                     GAIQSIRVARPVAVDKTIIESWTFRLVGAPPELLQRTTMYNRLINSPFSVVGHDDLNA
                     YRGIQEGLHAGGNPWVSLHRNFCADEIGERDRTVIGTSEISMRNQYRAWARYMTLTM"
BASE COUNT          760 a         1758 c         1907 g          831 t
ORIGIN      
        1 ggcatcgagg cggacgaggc cgagccgcca gggcagacgc tcgcggtaga agggatcgtt
       61 gctgtgatgc aggacggtct cgctgagcag ctcgccctcg ccggtctgcg gcgtccacct
      121 cagcgccgac gagaggcagc ggtggcaggc gtcgcgcggc ggatattgga ccgcaccgca
      181 ctgctggcag acctgcagct cgaagcggcc ctccgccgcc gccgccgtca tgccgagcgc
      241 ggtgcgcccg cgcgcccagg gcggcaggtt catctgccgc gtgcgcagca ccggattctt
      301 cttgcgcggc ttcatcagcg gcatcgtcac ggcgcgctcc ttcgcgcagg gctcgccgcg
      361 acgggccagc atccgaacgc caccgccgcg gctttcacga cgcccgcccc aggatgaccg
      421 cgccggtgca gaggcaccgg tcgtaggtga ccatgccgaa gccgccgacc aggccgagct
      481 cggcatcggc gacggcgcgc tcgccggcct ggccggtgag ctggcggatc gcttcggtca
      541 tgccgagaaa gccgccggcg cagcccgcct gtcccgtgga gagctggcca ccgccggtgt
      601 tgttcggaaa cgtgccgtca tgggtgagcg tccggctgcg cacgaattcg gcgccctcgc
      661 cttcgccgca aaagcccagg ccctcgaaat gaagcatgac gacgaccggg tagtcgtcgt
      721 agctctgcac gaaatcgatc gcgtcgggcc gcacgccggc ctgcgcgaac aggtcgtcgc
      781 ggtcgacgcg ccagccaccg cggatgagga tcggatcgtc ggcataggcg ttgtgtcgct
      841 cgatggcacc gcgcagcagc acgtggccca ggccgagatc gcgggcacgt tcctcgctca
      901 tcaccaggaa ggcatcggca ccggcgcagg gcatgacgca gtcgaacagg tggatcggct
      961 cggcgatggg ccgcgccgcc atgtaggcgt cgatcgagag cggcgtcttg aagagcgcgt
     1021 tcgggttctt cagcgcgttc gtgcgctggg ccacgcagat gcgcccgaag tcctcgcgcg
     1081 tcgcgccgta ggcgcgcatg tagtgcgcgg tgatgaaggc gaagatcgag ttcgggccgc
     1141 ccgacccgta gggatagctg gcatcgcgcg cgaaatgcga gaagccgccg agcatctgcc
     1201 ggaacgagtc gacgtggttg gtgtcggcgc cgatgcaggc gacgacgtcg gcgtcgcccg
     1261 cctgcaccgc gcgcgcggcg cggcgcagcg ccatcacgcc ggaggcgccg ccggtgggga
     1321 tgtgatcgag ccagcgcggg ctcaggccca ggtgctgcgt cacgccgacc gccgtgtcgg
     1381 ggccgagcga gaagctcgag atcgcgaggc cgtcgatctg ctccttggcg atgcccgact
     1441 cgcgcaccag cgcctgcatc gcctggccga tgaaccagtg cgcgccctgg gtcgaatagc
     1501 ggacgtaggg caccgtcacc ggcacggcga cggcgacgcc ctcataggac tggcgcggca
     1561 ctgcgagccc tctaaggcct gttcacgcta gtacagcttg tcgccgctga tatcgagctt
     1621 gtagccgccg agatcgagcg tgtcgccggc atcgcccttg tcgccgctcg cctcgggctt
     1681 ttcgccgccg gtctccggct tgccgttgcc tgtctgcagc ttgccactgc ctgtctgcaa
     1741 cttgccgctg cctgtctgca gcctgccgct gcccgagtcc agcttgctgc tgcccgtctc
     1801 gagctcgccg gccgcggtcg cggccgcctg cagctccttc ttgcgccgac gcttcatctg
     1861 ccgggtgtcg atgcagttcg gcttgccggg cagggtcttg gcaagatcct tcagttcgcc
     1921 gcgctggatc ttctgcgaga cggtgaccgg cagcgcatcg acgaaggcga cataccccgg
     1981 cgccttgaag taggcgagcc gttccatcgc atagcgcacg atgctggccg cgatctgctc
     2041 gagcgccggc aggttgggcg ccttgcgcag cacgacgcag gcgagcacct cgtcgccacg
     2101 caccggatcg ggcgtcgccg cgacggcgct ggcggcgacc gcggggtgca tgtcgagcac
     2161 gctctcgacc tccaccgccg agatgttctc gccgctgcgc cggatcacgt tcttgcgccg
     2221 gtcgacgaag tgaaagtcgc cttcggccgt gcgctgcacg acgtcgccgg tgtggaacca
     2281 gccgccggcc caggcctcct cggtggccgg ctcgtccttc aggtagccga ggaagaaatc
     2341 gttgcgcgat tcggcgccgg cggcgcggac cagcaactcg ccggacgcac cgtcggcgac
     2401 gtcggcgcca tggtggtcga cgacgcgcgt ctcgacgaac ggctcggcgc ggccgaagca
     2461 gcgggtgccg acgtggcgcg gttcgcggtg cgccatgatg caggcggccg cgccggtctc
     2521 ggtcatcgcc caggcctcga gcagcgggaa gccgaagcgc tcctcgaagg cggcgtggtg
     2581 gcgcggatcg acgccggcgc cgaagccgaa gcgcacggcg tggtcgcgat cgtgcgggcc
     2641 gggcggcagg ccgagaagca tcgccggcat gacgcccagg tagtggacga tcgtcgcccg
     2701 gctgtcgtgc acgctttgcc accaggtggc cggatgaaag cggtcgagct ggacgatggc
     2761 gccgcccgag aggatcgccg ccatcgacga gaacgccagc gcattcatgt ggctgagcgg
     2821 cagcggcgtg atgatccgct cgacgtcggc acgcaccgcg gccagctcgc ccagcccgag
     2881 gtaccactcg ccggcacgca ggaagtagcc gttggcgagg cgacatccct tgggccggcc
     2941 ggtcgtgccc gaggtgtaga gaagcgcgca ttcgctggtc cggccgatcg cgtggcccgc
     3001 ctggcgtgcc ggagaacgtg ccgccgggat gcggccgagc gcctccggcc ggatcgtctc
     3061 gagctgggcg ccggcctcgt tggcggcggc acgcagtgcg ccctcgcggc ccggctgcac
     3121 gaccgccagc gacatctcgc tgtggccgat gaggtaggtc cattcggccg cccgcatgtc
     3181 ggcgtggatc ggcacgatgc tgatgccgag cgcgttgagc gccagccagt gggtgaagaa
     3241 cgccggccgg ttctcgagca ggaggccgac acggtgcccg tgcccgtagc cagcctgcgc
     3301 ataggccgcg cgcagccgct ccacctcgac ggccacttcg cgccagctgc gcgcgccggc
     3361 gtcgatgccg taggtcctgg ccgtctccgg ctcgatgcag aggaaatcgg cgtcgccgcg
     3421 tcgcgcggca acccgggcga aggcgccgtg gacggtcgtc gagtcggggg gcgcgatggc
     3481 gcggttggga tcgttggacg tcatatgaag agctggaccg ccggcagggc ggcgtcggaa
     3541 ttgatgagat cgacgcgctt ttgcaggatg cgcagggcgc cctgcgcatc gacgacgagg
     3601 cggtgccggc agacgccgac gaaggtgttc atctcgtcgc cctgcgactc ggtgtactgg
     3661 aagacggtgc gcacgagatg ctcgccggcg cctgccgccg gctcgacggt gggcgtctgc
     3721 agcaggtgca ggcttcgcgt cggcggctgc tgcgagaagg cccgcgggct cttcagccgc
     3781 tcgatgcgca ggtcgcgcag cagcttgtcc tcgtagaggt gcgaggtgtg gtcgaggccc
     3841 tccggctgcc cgggcaccag cggcacccag tagatcgcgt cgtcggtgaa gagggcattc
     3901 cactcgtcgt atcgcttctc gtcgagcagg cgcgcctcgt ggacgacgaa gtcgatcaac
     3961 cggcgctcgg agacgtcgtg gtgcggcacg gcgctcatcg taagggcccg gcgagggccg
     4021 ctttcgagcc cgggggcacg acgtgcggca gcgaacgaag cgggcgcggg gccaccatgg
     4081 tcacatcgtc agcgtcatgt agcgcgccca ggcgcggtac tggttgcgca tcgagatctc
     4141 gctggtgccg atgaccgtcc ggtccctttc gccgatctcg tcggcgcaga agttgcggtg
     4201 caggctcacc cacgggttgc cgccggcgtg caggccttcc tggatgccac ggtaggcgtt
     4261 caggtcgtcg tggccgacga cggaaaaagg cgagttgatg aggcggttgt acatcgtcgt
     4321 tcgctgcagc agctcgggcg gcgcgccgac caggcggaag gtccagctct cgatgatggt
     4381 cttgtcgacc gccaccggcc gggcgacgcg gatcgactgg atcgccccct tgatcgtcag
     4441 gttcgggtag tagacggtgt tgtggcgcac caggccgagg atctcggcgg ttcgctcttc
     4501 gccgtaggcc tccttcatcg ccttttcgta ggcggggatc gccgagtact tgctgtggat
     4561 gctgaagttg gcgcccgaaa agccgtgccc gttgtcgaag acgcgaacgc ccatgtcctc
     4621 gaagaacttg tagtcggaca tgaagggaac gaactgctcg atcgccatcg gcttgggcgc
     4681 gtcggcgggc tgccccagcc acatgcgctt ggcggtgccg gccgacgact cgtgaaccac
     4741 catggggtgc atggtgtcat tgaggttctc gacgtactgt ttccagttgc actggtgcat
     4801 gaagcgcagg cagccgccgg cgatctcgag cctgccttcg ggcgagcggt cggcgaggtt
     4861 gtcgatcgac gacagcgact cgccgaagta ctcctcgaag gccggcccga cgtcgttgag
     4921 cttgacgaag atgaagcccc ggtacagggc cacgttcttc accgtcgcca ggccgtggcc
     4981 ggcttcgcat tcgtgcagcg ccgtgccctc gtagccgttc ttcagcggca tcgacagcag
     5041 cgagccgtcg gtgcggtagg tccaggcgtg gtaggggcag cgaaacagct tgccggtgtt
     5101 gccgcagggc gcgctcacga ggcgcgagcc cttgtgcgcg cagcggttca tcagcacgcg
     5161 caccgagccg tcctggtgcc gcacgacgat cagcggccgg ccggcgatct cgttggtgat
     5221 gtagtcgccg ggctgcggca gctggctcgc gtggcc
//
LOCUS       DASRWS010000084         6211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1761480, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000084 DASRWS010000000
VERSION     DASRWS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761480"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFF72_01505"
     CDS             complement(<1..274)
                     /locus_tag="VFF72_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91859.1"
                     /translation="MRGPLGGWSGWAGAASVALALAACSSVSLRPAPVVVIATPVPVA
                     PSAAPAPQPTPAPPPVATPFPVAPRTALPPTPDVLPGPVVPPAIAAR"
     gene            481..978
                     /locus_tag="VFF72_01510"
     CDS             481..978
                     /locus_tag="VFF72_01510"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HZV91860.1"
                     /translation="MLGQIIKGTPIGVWILLVALVVLGLKQTQPRTIGSRRAAALPAA
                     FVVLSLAGVLGAFGASALGVVAWACGIAASLATGPRLLPRIRASWQAAGDTLHVAGSW
                     LPLLLIVSLFAVKYAAGISLAMHPALAAQAPFVVAFSFAYGVFSGLFAARGRQLWQVR
                     AAARS"
     gene            1023..2165
                     /locus_tag="VFF72_01515"
     CDS             1023..2165
                     /locus_tag="VFF72_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZV91861.1"
                     /translation="MTTNAGSAPAASTAVASWRVLAGRGLKVAAFCLVIAVVLIALFG
                     DAFGHAVVYSLCIGLSCWLFIDVGGDLIAPLLSMPDFGSDSVRAGWRGWIWMTVLVLV
                     GATLGYATGNGIANFLTGEHQPGPFNASLRQLRTVLVLAFVPAVTMTYFLISREIIAR
                     QRAEVETAQRHAAEQQLKLLESQLEPHMLFNTLANLRVLIGIDATRAQAMLDRLIAFL
                     RASLEGSRARTHPLAAEFERLRDYLALMQVRMGERLQTRLDLPAGLESAQVPPLLLQP
                     LVENSIKHGLEPKVAGGRIEVSAAREGDALVLRVRDSGVGFDALAAGDGRYGLAHIRE
                     RLATLHGERARFEIVADDAPDGGTLATLRLPFSTDAPEPVAAPPSR"
     gene            2162..2968
                     /locus_tag="VFF72_01520"
     CDS             2162..2968
                     /locus_tag="VFF72_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZV91862.1"
                     /translation="MKARALIAEDEPLLAESLRGELAALWPALEIAATVGDGAAAVEA
                     ALALRPEVVFLDIRMPGMNGLEAAQALAEDWPEDVPFPLLVFVTAYDQYALQAFERAA
                     VDYVLKPVQTARLAATVRRLQDALAAKATSPPAVELGDVVERLRGLLAPGSPAAAPRA
                     EPLRVVQVGVGNAIHMVPIDDVLYFEAADKYVRVVTADREHLIRTSLRELLPRLDASR
                     FWQIHRGTVVRADAIVSVERDESGRATLKTQGRPDRLVVSRLYAHRFKAM"
     gene            3167..4969
                     /locus_tag="VFF72_01525"
     CDS             3167..4969
                     /locus_tag="VFF72_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZV91863.1"
                     /translation="MSTDTDTAPASNFLRHVIEHDLAEGTYATRRFAGTPGDAAHQAA
                     GPLDAARIRTRFPPEPNGYLHIGHAKSICLNFGLARDYGGICHLRFDDTNPEKEEQEY
                     VDAIVDAVHWLGFDWHAPEPARPDEATTSHLYFASSYFDFMYRAAEALVMAGKAYVDE
                     QSPEEMRQSRGDFATPGRNSAWRDRAPADNLARLREMKDGQLADGAATLRARIDMASP
                     NINLRDPALYRIKRATHHATGDRWCIYPMYTFAHPIEDALENITHSICTLEFEDQRPF
                     YDWLLDTLCELGLLAQPRPHQYEFARLNVTYVVTSKRKLRQLVDEKIVAGWDDPRLPT
                     IAGLRRRGYTPESIRLLCERAGTSKAGGWTDYASLDLALRDDLDEKAPRAMAVLEPLR
                     LKLVNWVEIFGDAAHRESCTAPAHPHRPELGERRFSLGPELWIERDDFAEVPAKGFFR
                     LFPGNRVRLKYGHIVECTGCEKNDAGEITAVLARIVPDTRSGTPGADAVKVKGTITWV
                     GVDDALDAEVRLYDRLFTDPQPDAGGGDFKACLNPASLRVMTAVVEPGLAGIVADARL
                     QFERHGYFVADRVEHRAGHPVFNRIAPLRDTWAK"
     gene            complement(5101..5631)
                     /locus_tag="VFF72_01530"
     CDS             complement(5101..5631)
                     /locus_tag="VFF72_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91864.1"
                     /translation="MAEKTRAPVLTGLFPDRDSAERAYQSVSDRGYTRDDVDVVMSDD
                     TRTRHFGTTEGRVTELGNKAAEGAGIGGAIGGSLGAIAAAIAAVGTTLALPGLGLVIA
                     GPLAAAVAGAGAGAATGGIVGALIGWGIPEERVKEYDAGIRGGGILMAVRPRNDDDAS
                     YFEDSWKRHRGEHVYR"
     gene            complement(5826..>6211)
                     /locus_tag="VFF72_01535"
     CDS             complement(5826..>6211)
                     /locus_tag="VFF72_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HZV91865.1"
                     /translation="ALAWAVVLVVILGLDAVWLGLVAKDFYKREMGSLMSDSVRVAPA
                     VVFYLLYPVALVYLALLEAPAAWTDALLRAAVLGLAAYGAYDLTNMSVVRGWPIGLSL
                     VDWAWGTVAAVIAAAAGYAVTWGRV"
BASE COUNT          889 a         2312 c         2154 g          856 t
ORIGIN      
        1 agcgcgcggc gatggccggc ggcacgacgg ggccgggaag aacgtcgggt gtcggcggca
       61 acgccgtccg gggcgccacc ggaaaaggcg tggcgacggg aggcggcgcg ggcgtcggct
      121 gcggcgccgg cgcagccgac ggagcgaccg ggaccggcgt tgcgatgacg acgaccggcg
      181 ccggccgcag cgacaccgag ctgcaggcgg ccagcgccag cgcgacgctc gcagcccctg
      241 cccatcctga ccacccaccg aggggtcctc gcatcgcgac tcctggccgc cccgggacgg
      301 cgcggcggcg ccaatatagc cgccagccgc aggcggccgc gcgaaaggcc ctcgcttcgc
      361 gccggatcga cgccgtttcg cgccgtcgcc gtgcggttcg tcgcagccgt gtcgaaccgc
      421 gtcggcgcac cgggcaaagt gccggcatcg cgcctcgggc gccttgccac ggagaacctc
      481 atgcttgggc agatcatcaa gggcacgccg atcggcgtct ggatcctcct cgtcgcgctc
      541 gtcgtcctcg gcctgaagca gacgcagccg aggacgatcg gcagccgccg cgccgccgcg
      601 ctgcccgccg cgttcgtcgt cctctcgctc gctggcgtcc tcggcgcctt cggtgccagc
      661 gccctcggcg tcgttgcctg ggcgtgcggc atcgccgcgt cgctggcgac cggtccgcgc
      721 ctcttgccgc gaatccgcgc ctcgtggcag gccgcgggcg acacgctgca cgtcgccggc
      781 agctggctgc cgctgctcct catcgtgtcg ctcttcgccg tcaagtacgc ggccggcatc
      841 agcctggcga tgcatccggc gctcgccgcg caggcgccgt tcgtggtggc cttcagcttc
      901 gcctacggcg tcttcagcgg cctgttcgcg gcgcgcggcc ggcagctctg gcaggtgcgt
      961 gccgcggcgc ggagctagac ggggcacgac gagtcgacac ccgcgtgcaa gagaatcggg
     1021 cgatgacgac gaacgcaggc tccgcgccgg ccgcttccac cgccgtcgcc agctggcgcg
     1081 tgctggccgg ccgcggcctc aaggtcgccg ccttctgcct ggtcatcgcc gtggtcctca
     1141 tcgccctgtt cggcgatgcc ttcggccacg cggtcgtcta ctcgctgtgc atcggcctga
     1201 gctgctggct gttcatcgac gtcggtggcg acttgatcgc gccgttgctg tcgatgcccg
     1261 atttcgggtc ggactcggtg cgtgccggct ggcgcggctg gatctggatg acggtgctcg
     1321 tgctcgtcgg cgcgacgctc ggctacgcga ccggcaacgg catcgccaac ttcctcaccg
     1381 gcgagcacca gcccggtccg ttcaacgcca gcctgcgcca gctgaggacg gtgctcgtcc
     1441 tggctttcgt gccggccgtg acgatgacct acttcctcat ctcgcgggag atcatcgccc
     1501 ggcagcgggc cgaggtcgag acggcgcagc gccatgccgc cgagcagcag ctcaagctgc
     1561 tcgagtcgca gctcgagccg cacatgctct tcaacaccct ggccaacctg cgcgtgctga
     1621 tcggcatcga cgcgacgcgc gcgcaagcca tgctcgaccg cctgatcgcc ttcctgcgcg
     1681 cttcgctcga aggctcgcgc gcccgcaccc atccgctcgc cgccgagttc gagcgcctgc
     1741 gcgactatct cgcgctgatg caggtgcgaa tgggcgagcg actgcagaca cggctcgacc
     1801 tgcccgccgg cctcgagtcg gcccaggtgc cgcccttgct gctgcagccg ctggtcgaga
     1861 acagcatcaa gcacggcctg gagcccaagg ttgcgggagg caggatcgag gtgagcgccg
     1921 cgcgcgaagg cgacgcgctg gtgctgcgcg tgcgcgacag cggcgtcggc ttcgacgcgc
     1981 tcgccgcggg cgacggacgc tacggcctgg cgcacatccg cgagcgcctg gcgacgctcc
     2041 acggcgagcg cgcccgcttc gagatcgtcg ccgacgatgc gccggatggc gggacgctgg
     2101 cgacgctgcg cctgcccttt tccaccgacg cgcccgaacc ggtcgcggcg ccgccgtcgc
     2161 gatgaaggcg cgcgcgctga tcgccgagga cgagccgctg ttggccgaga gcctgcgcgg
     2221 cgagctggcc gctctctggc ccgcgctcga gatcgcggcg acggtcggcg acggcgcggc
     2281 cgcggtcgag gcggcgctgg cgctgcgacc cgaggtcgtc ttcctcgaca tccgcatgcc
     2341 gggcatgaac ggcctcgaag cggcgcaggc gctggccgag gactggcccg aggacgtgcc
     2401 gtttccgctg ctcgtcttcg tcaccgccta cgaccagtac gcgctgcagg cgttcgagcg
     2461 ggccgccgtc gactacgtcc tgaagccggt gcagaccgct cgcctcgcgg cgacggtgcg
     2521 caggctgcag gacgcgctcg cggccaaggc gacatcgccg ccggcggtcg agctcggcga
     2581 tgtcgtcgag cgactgcgcg gcctgctcgc cccgggcagc ccggcggccg cgccgcgagc
     2641 cgagccgctg cgggtggtgc aggtcggcgt cggcaacgcg atccacatgg tgccgatcga
     2701 cgacgtgctc tacttcgaag ccgccgacaa gtacgtgcgg gtggtgacgg ccgatcgcga
     2761 gcatcttatc cggacctcgc tgcgcgagct gctgccacgc ctcgacgcga gtcgcttctg
     2821 gcagatccat cgcggcaccg tcgtgcgcgc cgacgccatc gtgtcggtcg agcgcgacga
     2881 atcgggccgg gcgacgctga agacacaggg gcggcccgac aggctcgtcg tcagccgcct
     2941 ctacgcgcac cgcttcaagg cgatgtaggc gagcgagaga ggaccgacgg cgacatggca
     3001 acgcgtcgac gtcggttctg gagcgctcgc gcgcgacggc gagttgccgt cttcgcgcga
     3061 ggagcgatcc ccgcttcgct tcggtgcagg gcagcgtcgc gccggcgagc tgcgacctcg
     3121 cctgcctaga atcagcgcgc cgccgatgcg ccgcggcacc tttcgcatga gcaccgacac
     3181 cgacaccgcc cccgccagca atttccttcg ccacgtcatc gagcacgacc tcgccgaagg
     3241 cacctacgcg acgcggcgct tcgccggcac gccgggagac gcggcgcatc aggcggccgg
     3301 gccgctcgac gcggcgcgca tccgcacccg cttcccgccc gagccgaacg gctacctgca
     3361 catcggccac gccaagagca tctgcctcaa cttcggcctg gcgcgcgact acggcggcat
     3421 ctgccacctg cgcttcgacg acaccaatcc cgagaaggaa gagcaggaat acgtcgacgc
     3481 catcgtcgac gcggtgcact ggctcggctt cgactggcat gcgcccgagc cggcccgacc
     3541 cgacgaggcg acgacgtcgc acctctactt cgcgagcagc tatttcgact tcatgtaccg
     3601 cgccgccgag gcgttggtca tggccggcaa ggcctatgtc gacgagcaga gccccgagga
     3661 gatgcggcaa agccgcggcg acttcgccac gcccggtcgc aacagcgcct ggcgcgaccg
     3721 cgcaccggcc gacaacctcg cccgcctgcg cgagatgaag gacggccagc tcgccgacgg
     3781 cgcggcgacg ctgcgcgcca ggatcgacat ggcgagcccg aacatcaacc tgcgcgatcc
     3841 ggcgctctac cgcatcaagc gcgcgactca tcacgccacg ggcgaccgct ggtgcatcta
     3901 cccgatgtac accttcgcgc atccgatcga ggacgcgctc gagaacatca cccattcgat
     3961 ctgcacgctc gagttcgagg accagcggcc gttctacgac tggctgctcg acacgctctg
     4021 cgagctcggc ctgctggcgc agccgcggcc gcaccagtac gagttcgcac ggctcaacgt
     4081 cacctatgtc gtcaccagca agaggaagct caggcagctc gtcgacgaga agatcgtcgc
     4141 cggctgggac gacccgcgcc tgccgacgat cgccgggctg cgccggcgcg gctacacgcc
     4201 cgaatcgatc cgcctgctct gcgagcgcgc cggcaccagc aaggccggcg gctggaccga
     4261 ctacgcgagc ctggacctcg cgctgcgcga cgacctcgac gagaaggcgc cgcgtgccat
     4321 ggcggtgctc gagccgctgc gcctgaagct cgtcaactgg gtcgagatct tcggcgacgc
     4381 ggcgcatcgc gagagctgca cggcgccggc acacccgcat cggcccgagc tcggcgagcg
     4441 ccgcttctcg ctcggtcccg agctctggat cgagcgcgac gacttcgccg aggtgcccgc
     4501 gaagggcttc ttccggctct tcccgggcaa tcgcgtccgc ctgaagtacg gccacatcgt
     4561 cgagtgcacc ggctgcgaga agaacgacgc cggcgagatc acggccgtgc tcgcacggat
     4621 cgttcccgac acgcgcagcg gcacgcccgg cgccgacgcg gtgaaggtca agggcacgat
     4681 cacctgggtc ggcgtcgacg atgccctcga cgccgaggtc cgcctctacg accgactctt
     4741 caccgacccg cagcccgacg ccggcggcgg ggacttcaag gcctgcctga atccggccag
     4801 cctccgtgtc atgactgccg tcgtcgagcc ggggctggcg ggcatcgttg ccgatgcacg
     4861 actgcagttc gagcgccacg gctacttcgt cgccgaccgc gtcgagcacc gagccgggca
     4921 ccccgtcttc aatcgcatcg cgccgctgcg cgacacctgg gcgaagtagc gcccgtctcc
     4981 ggcgccggtc acttcggcgc gcgcggtcgc cgacgtcgtc gtcgaggcca catcgccgtc
     5041 accaacccgg ctccgtcggc aggactcgtc acggaggacg gccacgcggc gagccgatcc
     5101 ctatcggtag acgtgctcgc cgcggtggcg cttccacgag tcctcgaagt agctcgcgtc
     5161 gtcgtcgttg cggggacgca ccgccatcag gatgcccccg ccgcggatgc cggcgtcgta
     5221 ttccttgacc cgttcttccg ggatgcccca gccgatcagt gcgccgacga tgccgccggt
     5281 cgccgcgccg gcgccggcgc cggccacggc cgcagcgagc ggcccggcga tgaccaggcc
     5341 caggccgggc agcgccaggg tcgtgccgac ggcggcgatc gccgcggcga tggcgcccag
     5401 gctgccaccg atggcgccgc cgatgccggc gccctcggcg gccttgttgc cgagctcggt
     5461 gacgcggcct tcggtcgtgc cgaaatggcg cgtgcgggtg tcgtccgaca tgacgacgtc
     5521 gacgtcgtct cgcgtgtagc cgcggtcgga caccgactgg tacgcgcgct cggcgctgtc
     5581 gcggtcggga aacaggccgg tcagcaccgg cgcgcgggtc ttttccgcca tggcgtcgct
     5641 cctcgttgca agtgtcgaag gcaaggatcg cccgcgccgc agctcgaccg tagcggcgcg
     5701 ctccttccgg gcgggtcgtc cgcgacgtgc cggcggcgtg gggcggctcc tacaccggga
     5761 gcgactgtcg cactgagcct gacccgcgcg accgcgtggg cgacgcgcgt cgcctcgacg
     5821 agcccctaga cgcggcccca ggtcacggcg tacccggcgg cggcggcgat gaccgcggcc
     5881 accgtccccc atgcccagtc gaccaggctc aggccgatcg gccagccgcg cacgaccgac
     5941 atgttggtga ggtcgtaggc accgtacgcg gcgagtccga gcacggccgc gcgcagcagc
     6001 gcatcggtcc aggcggcagg cgcctcgagc agcgccaggt agacgagcgc gaccggatag
     6061 agaaggtaga agacgacggc cggcgcgacg cgaaccgaat cgctcatcaa cgaacccatc
     6121 tcgcgcttgt agaaatcctt ggcaaccagg cccagccaga ccgcatcgag gccgaggatg
     6181 accaccagca cgacggccca ggcgagcgcg a
//
LOCUS       DASRWS010000085         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_36135, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000085 DASRWS010000000
VERSION     DASRWS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36135"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFF72_01540"
     CDS             complement(<1..381)
                     /locus_tag="VFF72_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZV91866.1"
                     /translation="MPTANRQIHLVSRPSGEASADNFRVVDASVGEPVDGQVLVRHRF
                     LSLDPYMRGRMNDAKSYAAPQPLGQVMQGGTVGEVVASRHPDYRPGDAVVGFGGWQQF
                     SIVDAATPGILRKVDTTHVSMSAYL"
     gene            complement(395..832)
                     /locus_tag="VFF72_01545"
     CDS             complement(395..832)
                     /locus_tag="VFF72_01545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZV91867.1"
                     /translation="MTEVVHIPFAELLGLEVVRFERDEADLALELRDELCNSWSVAHG
                     GATMTLLDVAMAHAARSPDADGKVDPCGIVTIEMKTTFMQPGTGRLLARARRMHRTAS
                     LAFCEATVLGGDGKLVAQATGTFKFVRALAVGGKRIQRLNASD"
     gene            complement(951..1742)
                     /locus_tag="VFF72_01550"
     CDS             complement(951..1742)
                     /locus_tag="VFF72_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV91868.1"
                     /translation="MSTPVRQLFDLTGRTALVTGGSRGLGLQMAEALGEAGARVMLTS
                     RKASDLEEARAHLAARGVQALWVAADASQPSEVVRVAREAIEKLGQVDILVNNAGATW
                     GAAAEDHPLEAWDKVMNLNVRSLFLMSQAIGKHSMIPRRAGKIINVASIAGLAGNSAD
                     MAMVAYNTSKAAVVNFTRTLAGEWGKYGINVNALAPGMFPSKMTKGTISRIGEKELAG
                     HAPLLRIGDDDDLKGATLLFASQAGKHITGQVLPIDGGVSAIIAA"
     gene            complement(1780..1986)
                     /locus_tag="VFF72_01555"
     CDS             complement(1780..1986)
                     /locus_tag="VFF72_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91869.1"
                     /translation="MRISPARVEVLVWVLVYGGLFVACLGFALQRDGHAEGWAVLGGG
                     LAIALAGVVLIWVRARMNGHGERP"
     gene            complement(1983..>2365)
                     /locus_tag="VFF72_01560"
     CDS             complement(1983..>2365)
                     /locus_tag="VFF72_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZV91870.1"
                     /translation="AALQSEIDATALAAAASPSLAGYAQQLKAAAAQLGAATSAAWAT
                     GSADEALANATPYLQAFGHVVIAWMWLSVATIAVRAVDDAAQRGRLAACRYFFAYELP
                     KIAAWLGVVERRDDTCRTLAEESF"
BASE COUNT          376 a          814 c          806 g          369 t
ORIGIN      
        1 gaggtacgcc gacatcgaca catgcgtcgt gtcgaccttg cgcaaaatcc ccggagtggc
       61 tgcgtcgaca atcgagaact gctgccatcc gccgaagccg accaccgcgt cgcccgggcg
      121 gtagtccgga tgacgagagg ccaccacctc gccgaccgtg cctccctgca tcacctgacc
      181 gagcggctgc ggcgccgcgt agctcttggc gtcgttcatc cgcccacgca tgtacggatc
      241 gaggctcagg aatcgatggc gaaccagcac ctggccgtcg acgggctcgc cgacgctcgc
      301 atcgacgact cggaagttgt cggcagaagc ttcgccgctg gggcgcgaca cgaggtggat
      361 ctggcgattt gctgtgggca tggtgcagga tctttcagtc ggaggcgttg agtcgctgga
      421 tgcgcttgcc gccaaccgcg agcgcgcgaa cgaacttgaa ggtgccggtc gcctgcgcga
      481 cgagcttgcc gtcgccgccc agcacggtcg cttcgcaaaa ggcgagcgag gcggtgcggt
      541 gcatgcgccg ggcacgggcc agcaagcggc ccgtgcccgg ctgcatgaac gtcgtcttca
      601 tctcgatcgt gacgatgccg caggggtcga ccttgccgtc ggcatcgggg cttcgcgcgg
      661 cgtgggccat ggcgacgtcg agcagcgtca tcgtcgcgcc accgtgggcg acgctccacg
      721 aattgcacag ctcgtcgcgc agctcgagcg cgagatccgc ctcgtcgcgc tcgaagcgca
      781 cgacctcgag cccgagcagc tcggcgaacg gaatgtgcac gacttccgtc atggttgcgc
      841 ttcgcggcag gcgctcctcc atcgggagga cgcccgctgc gcgacctcgg tcgaccctgt
      901 gtcgaccgtc gatgtgcaga ccagcgacgg cgatgcagaa aggagcgtca tcacgccgcg
      961 atgatggccg acacgccacc gtcgatcggc agcacctggc cggtgatgtg cttgccggcc
     1021 tgcgaagcga acagcagcgt cgcgcccttg aggtcgtcgt catcgccgat gcgaagcagc
     1081 ggcgcgtgac cggcgagttc cttctcgccg atccgggaga tcgttccctt cgtcatcttg
     1141 ctcgggaaca tccccggcgc gagggcgttg acgttgatgc cgtacttgcc ccattcgccg
     1201 gccagggtgc gcgtgaagtt gacgaccgcg gccttgctcg tgttgtaggc gaccattgcc
     1261 atgtcggcgc tgttgccggc gaggcccgcg atcgaggcga cgttgatgat cttgccggcg
     1321 cgccgaggaa tcatgctgtg cttgccgatc gcctggctca tcaggaagag cgagcggacg
     1381 ttcaggttca tcaccttgtc ccaggcctcg agcgggtgat cctcggccgc ggcgccccag
     1441 gtcgcgccag cgttgttgac gagaatgtcg acctggccga gcttttcaat cgcttcccgg
     1501 gcgacgcgga ccacctcgct cggctggctc gcatcggcgg cgacccagag agcctgcacg
     1561 ccacgcgccg ccagatgcgc cctcgcctct tcgaggtcgg acgccttgcg cgacgtgagc
     1621 atgacgcggg cgcccgcctc gccgagcgct tctgccatct gcaagccgag gccgcgcgag
     1681 ccgccagtca cgagcgcggt gcgacccgtc aggtcgaaga gctgtcgcac gggagtgctc
     1741 atgtggatct ccttttcaag aaattcggct cgacgcggct cagggccgct cgccgtgccc
     1801 gttcatgcgc gcgcggaccc agatgaggac gacaccggcg agcgcgatgg ccagcccccc
     1861 gccgagaacg gcccagccct cggcgtgccc gtcgcgctgc aatgcgaagc cgaggcaggc
     1921 gacgaagagg cccccgtaga caaggaccca gacgagcact tcgacccgtg ccggcgagat
     1981 cctcaaaacg actcctcggc aagcgtgcgg caggtgtcgt cgcgtcgctc gacgacgccc
     2041 agccacgccg cgatcttcgg cagctcgtag gcgaagaaat agcggcacgc cgccagacgg
     2101 ccgcgctgcg cggcatcgtc cacggcccgg acggcgatcg tcgccacgga cagccacatc
     2161 caggcgatga cgacgtgtcc gaaggcctgc agatagggcg tggcgttggc gagcgcctcg
     2221 tcggccgagc cggtcgccca ggcggcgctc gtcgccgcac cgagctgggc ggcggcggcc
     2281 ttcagctgtt gcgcgtatcc agcgagcgac ggcgatgcgg ccgccgccaa agcggtggca
     2341 tcgatttcgg actgcagcgc cgcca
//
LOCUS       DASRWS010000086         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2050016, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000086 DASRWS010000000
VERSION     DASRWS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050016"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="ligD"
                     /locus_tag="VFF72_01565"
     CDS             <1..973
                     /gene="ligD"
                     /locus_tag="VFF72_01565"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZV91871.1"
                     /translation="AARSGPRSAPLAAKVTAAKQARGEAEVRGIRISHPDRVIDASTG
                     ITKLELVDYYLAVARLILPHLAKRPVSLVRAPAGIGGHLFFQKHAAALKIPDLKTLDR
                     SIAPDLEPMVEVDSFTALIGAAQANVVEFHTWNATTRDTTRPDRIVFDLDPGKGLAWK
                     QVQEAAELTRSLLEQLGLVAFLKTSGGKGLHVVVPIAPKDDWDTVKALSKAIVEHMAT
                     VIPERFVAKSGPKNRVGRIFVDYLRNNFGATTASAWSARARPGLGISVPCDWNELGRL
                     TGGAHWTIRTAHERIEEKTDAWRDYAKKARQGTVKAMKVLGVGPEAD"
     gene            complement(1079..>3570)
                     /locus_tag="VFF72_01570"
     CDS             complement(1079..>3570)
                     /locus_tag="VFF72_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV91872.1"
                     /translation="ARAARFLRFAQGEPSHNLLAAVLPELRGELRSVLFQAAQGHARI
                     DAAPIPLVIDGRRVQVRTTAQAVRHPDWPAEMLMVTFDEATIAEDVDVGDSHDGAEVG
                     RLQVELHRKSDQLRTTIEQYETSAEELKASNEELQAINEELRSATEELETSKEELQST
                     NEELITVNQELKTKIDETIEINDDLRNLIGSTDIATVFVDPDMRIKRFTPAAAAIFNI
                     IAGDIGRPLFDITHRLDYGGLADDARAVFSSLKSIEREVASDDGRRLLARLLPYRTAE
                     DRIGGAVLTFVDVTSLRQAEAGLHIGEERMRLIAETMTDFAILTLDLEGRFTGWSAGA
                     ERLFGYAADEVVGDSFDILFSPEDRERGAAARELRIARTSGRAPDERWMQRKDGSLFY
                     ASGVTTPLRAEGVEGYAKICRDMTHIRTAAQTQAMRLDEAEQGRERARADAAMKAEFL
                     AVMSHELKHPLNLISVNAQLLTVLPEAQGLPAVQRAAQTIQRTVQGQGRVIDDLLDMS
                     RTNVGKLAVNRVPLLIVEAIQPCMTWALAESRRKGVRLFVEGLDDPVMIDGDPVRIEQ
                     ICWNLLSNAIKFTPQRGSITVRLSRDGEDALLCVEDTGRGIAPEFLPQIFEMFKQEES
                     ARTRVEGGLGIGLALARSLVELHGGRITAESAGPGLGASFKVWLPLHQRTGFSPLDEA
                     ASPPSGWLAGLRVLVVDDTPDALETFAFLLEYEGATVVQAQSAAEALRLADNQDFDLL
                     ISDIGMPQMDGYELIAELRSRPRTATLPSIALTGYGSSEDRQRALSAGFDAHLDKPVE
                     LDQLRVVVAAVRAAAANGTTASPDVGDGASG"
BASE COUNT          545 a         1223 c         1233 g          569 t
ORIGIN      
        1 ggccgcgaga tcgggcccgc gatccgcccc gctggcggca aaagtcacgg ccgcgaaaca
       61 ggcgcgcggc gaggccgagg ttcggggcat ccgcatcagc catcccgacc gggtcatcga
      121 cgcctcgacc ggcatcacca agctcgagct cgtggactac tacctcgcgg tggcacggct
      181 gatcctgccg cacctcgcca aaaggccggt ctcgctggtg cgggcgccgg ccggcatcgg
      241 cggccacctg ttcttccaga agcacgccgc ggcgctgaag atccccgacc tgaagaccct
      301 cgaccgctcg atcgcacccg atctggagcc gatggtcgag gtcgattcat tcaccgcgct
      361 gatcggcgcg gcgcaggcca acgtcgtcga attccacacc tggaacgcga cgacgcgtga
      421 cacgacgcgg cccgatcgca tcgtcttcga cctcgatccc ggcaagggcc tggcctggaa
      481 acaggtccag gaagccgccg agctgacccg gtcgctgctc gagcagctgg gcctcgtggc
      541 cttcttgaag acgagcggcg gcaagggcct gcacgtcgtc gtgccgatcg cgccgaagga
      601 cgactgggac acggtgaagg cgctgtcgaa ggcgatcgtc gagcacatgg cgaccgtcat
      661 ccccgagcgt ttcgtggcca agagcgggcc gaagaaccgg gtcggcagga tcttcgtcga
      721 ctacctgagg aacaacttcg gcgcgaccac ggcgtcggcc tggtcggcgc gggcgcggcc
      781 cggtctgggc atctcggtgc cttgcgactg gaacgagctc ggccggctga ccggcggtgc
      841 gcactggacg atccgcacgg cccacgagcg catcgaggaa aagaccgacg cctggcgcga
      901 ctacgcgaag aaggcgcggc agggcaccgt caaggcgatg aaggtgctgg gcgtcggtcc
      961 cgaggcggat tgaggcacgc ccggcccggc gtggcgagcg ggccgactgc gccgtgatcg
     1021 tgacctccga gcgcgaaccg ggcgtgcagc gccgaccgtg gcgagacggc ttcggacttc
     1081 agccgctcgc gccgtcgcca acgtccggcg atgccgtcgt gccgttcgcg gcggccgcgc
     1141 gcacggctgc gacgacgacg cgcaactggt cgagctcgac cggcttgtcg agatgcgcat
     1201 cgaagcccgc gctcagggcg cgctgccggt cttcgctgct gccgtagccg gtcagcgcga
     1261 tcgagggcaa ggtggccgtg cgcggccggc tgcgcagttc ggcgatcagc tcgtagccgt
     1321 ccatctgcgg catgccgatg tcggagatga ggagatcgaa atcctggttg tcggcaagac
     1381 gcagtgcctc ggcggcgctt tgcgcctgca cgaccgtcgc gccttcgtac tcgagcagga
     1441 aggcgaacgt ctcgagcgca tcgggcgtgt cgtcgacgac gagaacgcgc aggccggcga
     1501 gccatccgga cggagggctt gcggcctcgt ccagcggcga gaagccggtg cgctggtgca
     1561 gcggcagcca gaccttgaaa ctcgcgccca gtcccggccc cgccgattcg gctgtgatgc
     1621 gtccgccgtg cagctcgacc aggctgcgcg ccagcgccag gccgatgccc aggccgcctt
     1681 cgacccgcgt ccgcgccgac tcctcctgct tgaacatctc gaagatctgc ggcaggaatt
     1741 ccggggcgat gccgcgaccg gtgtcctcga cgcagagcaa tgcgtcttcg ccgtcgcgcg
     1801 acaggcgcac cgtgatcgat ccgcgctgcg gcgtgaactt gatcgcattg ctcagcaggt
     1861 tccagcagat ctgctcgatg cgcaccgggt cgccgtcgat catcaccgga tcgtcgagcc
     1921 cctcgacgaa gaggcgaacg cccttgcggc gcgactcggc cagcgcccac gtcatgcacg
     1981 gctggatcgc ctcgacgatc agcagcggca cgcgattgac ggcgagcttg ccgacgttgg
     2041 ttcgcgacat gtcgagaagg tcgtcgatca cgcggccttg cccctgcaca gtccgctgga
     2101 tcgtctgcgc ggcgcgctgc acggctggca ggccctgcgc ctccggcaac accgtcagga
     2161 gttgcgcgtt gacgctgatg aggttgagcg gatgcttcag ctcgtgcgac atgacggcga
     2221 ggaactccgc cttcatcgcc gcatcggctc gggcccgttc gcgcccctgc tcggcttcgt
     2281 cgagccgcat cgcctgcgtc tgcgcggcgg tgcggatgtg cgtcatgtcg cggcagatct
     2341 tggcgtagcc ctcgacgccc tcggcgcgca ggggcgtcgt cacgccgctg gcgtagaaga
     2401 gacttccgtc cttgcgctgc atccagcgct cgtcgggcgc ccggccgctc gtccgcgcga
     2461 tgcgcagctc cctcgccgcg gcgccgcgct cccggtcttc gggcgagaag aggatgtcga
     2521 aggagtcgcc gacgacctcg tcggcggcgt agccgaagag ccgctccgcg ccggcgctcc
     2581 agccggtgaa tctgccctcg agatcgagcg tcaggatggc gaaatcggtc atcgtctcgg
     2641 cgatcaggcg catccgctcc tcgccgatat gcaaccccgc ctcggcttgg cgaaggctgg
     2701 tcacgtcgac gaaggtcagc accgcgccac cgatccggtc ttcggccgtg cgatagggca
     2761 gaagccgcgc caggaggcgg cggccatcgt cgctggcgac ttcgcgctcg atcgacttca
     2821 gcgagctgaa gacggctcgc gcatcgtcgg cgaggccgcc gtagtcgagc cggtgcgtga
     2881 tgtcgaacag cgggcgaccg atgtcgccgg cgatgatgtt gaagatcgcc gcggccgccg
     2941 gcgtgaagcg cttgatgcgc atgtcggggt cgacgaagac ggtggcgatg tcggtcgagc
     3001 cgatcaggtt gcggaggtcg tcgttgatct cgatggtctc gtcgatcttg gtcttgagct
     3061 cctggttgac ggtgatcagc tcctcgttgg tcgactgcag ctcctccttg ctcgtctcga
     3121 gctcctctgt ggcagagcgc agctcctcgt tgatcgcctg cagctcctcg ttggatgcct
     3181 tcagctcctc ggccgacgtc tcgtactgct cgatcgtcgt ccgcagctgg tcgctcttgc
     3241 gatgcagctc gacttgcagg cgacccacct cggcgccgtc gtgcgaatcg ccgacgtcga
     3301 cgtcctcggc gatcgttgcc tcgtcgaacg tgaccatcag catctcggcc ggccagtccg
     3361 gatgacgcac cgcctgcgcc gtcgtgcgaa cctgtacccg ccggccatcg atgacgagag
     3421 gaatcggcgc ggcatcgatc cgggcatggc cttgcgcggc ctggaacagc accgaacgaa
     3481 gctcgccgcg caattcgggc agcacggccg cgagcaggtt gtgcgaaggc tcgccttggg
     3541 cgaaacgaag aaagcgcgcc gcgcgcgccg
//
LOCUS       DASRWS010000087         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3776374, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000087 DASRWS010000000
VERSION     DASRWS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3776374"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFF72_01575"
     CDS             complement(<1..390)
                     /locus_tag="VFF72_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZV91873.1"
                     /translation="MAKEKFERNKPHCNIGTIGHVDHGKTTLTAAITMTLAKSGGATA
                     KRYDEIDAAPEEKARGITINTAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPA"
     gene            complement(442..>2107)
                     /gene="fusA"
                     /locus_tag="VFF72_01580"
     CDS             complement(442..>2107)
                     /gene="fusA"
                     /locus_tag="VFF72_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZV91874.1"
                     /translation="KTGADFQMCLRTIRERLAVKAIPIQLPIGSEAALKGVVDLVRMK
                     AVVWDSEGLGANYHDEEIPADMKAACEEARHYLIENAVELDDEAMEAYLDGQEPSEAV
                     LKKCIRKAVLSGAFFPILAGSAFKNKGVQPLLDAVVDYLPSPVDIPPTPGIDYKTEEP
                     VTRRASDDEALSVLAFKIMDDPFVGSLTFCRIYSGKLETGMGLLNSTRDKRERVGRML
                     LMHSNNREDIKEAYAGDIVALAGLKDTRTGDTLCDPNKSPVILEKMNFPAPVIEIAIE
                     PKSKADQEKLGVALAKMVAEDPSFTVQTDQESGQTIMKGMGELHLDIKVDILKRTYKV
                     EANIGAPQVAYRESLGRRTEIDYTHKKQTGGTGQFARVKLVFEPGEPGSGFVFESKIV
                     GGSVPKEFIPGVEKGLESAKENGLLAGFPLIDFKATLIDGAFHDVDSSVLAFEIASRA
                     AFKELREKGAPKLLEPIMKVEVLTPDEYLGDVIGDLNSRRGQIQGTDQRGNAQVVTAF
                     VPLANMFGYIGNLRGMSQGRAQFTMQYDHYEAVPQHVADEVIKKYA"
BASE COUNT          291 a          702 c          683 g          431 t
ORIGIN      
        1 cgccggcacc cccacctggc gcgccagaag gatgtgctcg cgggtctgcg gcatcgggcc
       61 gtccgccgcc gacaccacca ggatcgcgcc gtccatctgc gccgccccgg tgatcatgtt
      121 cttcacatag tccgcgtgac cggggcagtc gacgtgcgcg tagtgacggt tcttggtctc
      181 gtactccacg tgcgccgtgt tgatcgtgat gccccgcgcc ttctcctccg gcgccgcgtc
      241 gatctcgtca taccgcttcg ccgtcgcccc accgctcttc gccagcgtca tggtgatcgc
      301 cgccgtcagc gtcgtcttgc catggtccac gtgaccgatc gtgccgatgt tgcagtgcgg
      361 cttgttgcgt tcgaactttt ccttggccat ttcaagctcc agccctgttt gggcttgtcc
      421 tcttactagg gttggaaggg gttaggcgta cttcttgatc acttcgtcgg cgacgtgctg
      481 cggcacggcc tcgtagtggt cgtactgcat ggtgaactgg gcgcggcctt gcgacatgcc
      541 gcgcaggttg ccgatgtagc cgaacatgtt ggccagcggc acgaaggcgg tgacgacctg
      601 ggcgttgccg cgctggtcgg tgccctggat ctggccgcgg cggctgttga ggtcgccgat
      661 gacgtcgccc aggtactcgt cgggcgtcag cacctcgacc ttcatgatcg gctcgagcag
      721 cttgggagcg cccttctcgc gcagttcctt gaaggccgcg cgcgaggcga tttcgaaggc
      781 cagcaccgag gagtcgacgt cgtggaaggc gccgtcgatc agggtcgcct tgaagtcgat
      841 cagcgggaag ccggccaaca ggccgttctc cttggccgac tccaggccct tctcgacgcc
      901 cgggatgaat tccttgggca ccgagccgcc gacgatcttg ctctcgaaca cgaagcccga
      961 acccggctcg cccggctcga acaccagctt gacgcgggcg aactggccgg tgccgccggt
     1021 ctgcttcttg tgcgtgtagt cgatctcggt gcggcggccg aggctctcgc ggtaggccac
     1081 ctgcggcgcg ccgatgttgg cttccacctt gtaggtgcgc ttcaggatgt cgaccttgat
     1141 gtccaggtgc agctcgccca tgcccttcat gatcgtctgg cccgactcct gatcggtctg
     1201 gacggtgaag gacgggtcct cggcgaccat cttggccagc gccacgccca gcttttcctg
     1261 gtcggccttc gacttcggct cgatcgcgat ctcgatgacc ggcgccggga agttcatctt
     1321 ctccaggatc accggcgact tgttgggatc gcagagcgtg tcgcccgtgc gggtgtcctt
     1381 caggccggcc agggcgacga tgtcgccggc gtaggcttcc ttgatgtcct cgcggttgtt
     1441 ggagtgcatc agcagcatgc ggccgacccg ctcgcgcttg tcgcgcgtcg agttcagcag
     1501 gcccatgccg gtctccagct tgcccgagta gatgcggcag aaggtcagcg agccgacgaa
     1561 cgggtcgtcc atgatcttga acgccagcac cgacagcgcc tcgtcgtccg acgccctgcg
     1621 ggtgacgggc tcttcggtct tgtagtcgat gcccggggtc ggcgggatgt ccaccggcga
     1681 aggcaggtag tcgaccaccg cgtccagcag cggctgcacg cccttgttct tgaaggccga
     1741 gcccgcgagg atcgggaaga aggcgcccga cagcacggcc ttgcggatgc acttcttcag
     1801 caccgcctcg ctcggctcct ggccgtcgag gtaggcttcc atcgcctcgt cgtcgagttc
     1861 gacggcgttc tcgatcaggt aatggcgagc ttcttcgcac gcggccttca tgtcggccgg
     1921 aatctcttcg tcgtgatagt tggccccgag cccctcggaa tcccacacca cggccttcat
     1981 gcggaccagg tcgacgacgc ccttgagggc ggcttccgaa ccgatcggca gctggatcgg
     2041 gatcgccttc accgccaggc gctcgcggat cgtgcgcagg cacatctgga agtcggcccc
     2101 ggtcttg
//
LOCUS       DASRWS010000088         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1186187, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000088 DASRWS010000000
VERSION     DASRWS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186187"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="der"
                     /locus_tag="VFF72_01585"
     CDS             <1..1028
                     /gene="der"
                     /locus_tag="VFF72_01585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZV91875.1"
                     /translation="GKKVLLAVNKAEGMAESPHLAEFHELGLGEPMAVSAAHGQGIGP
                     LVDAALGAALADAWPDDTTSPDAERGDDAPIRLAVAGRPNVGKSTLINAWLGEERLVA
                     FDQPGTTRDAIVVPFERDGTRFDLIDTAGLRRRGKVFEAVEKFSVVKTLQAIAEADVV
                     LLLLDATQGVTDQDAHIAGYIVESGRAVVVAINKWDAVDAYAREQLERSIAQRLPFLR
                     YATVLTISARHRRGFAAVWKAIAEARASAIRKMPTPVLTRLLLEAVQHQSPKRAGAFR
                     PKLRYAHQGGSNPPIVIVHGNSLEHVTDAYKRFLEGRIRAHFKLTGTPLRIEMRSSRN
                     PFVDKRD"
     gene            1107..1352
                     /gene="hfq"
                     /locus_tag="VFF72_01590"
     CDS             1107..1352
                     /gene="hfq"
                     /locus_tag="VFF72_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZV91876.1"
                     /translation="MSNKGQLLQDPFLNLLRKEHVPVSIYLVNGIKLQGHIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPGRAVNFQAQPEAPEQS"
     gene            1384..>2410
                     /gene="hflX"
                     /locus_tag="VFF72_01595"
     CDS             1384..>2410
                     /gene="hflX"
                     /locus_tag="VFF72_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZV91877.1"
                     /translation="MNAGHESAGPPARAILVGVDIGQKASFDASLDELALLAESAGDI
                     AVGRMVARRKAPDPAFFIGSGKADELRELVQLHQADAVLFDQALSPAQQRNLEKHLGV
                     AVADRTMLILEIFGERARSHEGKLQVDLARLQYLSTRLVRRWSHLERQRGGIGNRGGP
                     GEAQIELDRRMIGERIKNIKKQLEKVKRQRSTQRRARERNETFRVSLVGYTNAGKSTL
                     FNALSGADAYVADQLFATLDTTTRRLRMDEVARGVALSDTVGFIRELPHSLIEAFQAT
                     LQEAAEADLLLHVVDCANPELAEQMAEVARVLREIDAADLPQVVVFNKVDSLADTERP
                     RQRVDAME"
BASE COUNT          421 a          837 c          784 g          368 t
ORIGIN      
        1 ccggcaagaa ggtgctgctc gccgtgaaca aggccgaggg catggccgag tcgccccacc
       61 tcgccgaatt ccacgaactc ggcctcggcg agccgatggc ggtctcggct gcgcacgggc
      121 agggcatcgg gccgctcgtc gacgcggccc tcggcgcggc gcttgccgac gcgtggccgg
      181 acgacaccac gtcacccgac gcggaaaggg gagacgacgc accgatccgg cttgcggtgg
      241 cgggtcgccc caacgtcggc aaatccacgc tcatcaacgc ctggctcggc gaggagcgac
      301 tcgtcgcctt cgaccagccg gggacgaccc gcgatgcgat cgtcgttccg ttcgagcgcg
      361 atggaacccg cttcgatctc atcgacaccg ccggcctgcg ccggcgcggc aaggtcttcg
      421 aggcggtcga gaagttctcg gtcgtcaaga cgctgcaggc catcgccgag gccgatgtcg
      481 tcctgctgct cctcgacgcc acgcaaggcg tgaccgacca ggacgcgcac atcgccggct
      541 acatcgtcga aagcggccgt gccgtcgtcg tcgcgatcaa caaatgggac gccgtcgacg
      601 cctatgccag ggagcagctc gagcgctcga tcgcgcaacg gctgccgttc ctgcgctacg
      661 cgacggtgct gacgatatcc gcccggcacc ggcgcggctt cgccgccgtc tggaaggcga
      721 tcgccgaggc gagagcctcg gcgatccgca agatgccgac gccggtcctg acgaggctgc
      781 tgctcgaggc ggtgcagcac caaagcccca agcgtgccgg tgcctttcgc ccgaagctgc
      841 gctacgcgca ccagggcggc tcgaatccgc cgatcgtcat cgtccacggc aattccctcg
      901 agcacgtcac cgatgcctat aagcggttcc tggaagggcg gattcgtgcc cacttcaagc
      961 tgaccggcac gccgcttcgc atcgagatgc gctcctccag gaatcccttc gtcgacaagc
     1021 gcgactgagg catggcccca cgggccgccg cccgggaatc gctgtgttat ggtgactcgt
     1081 cacagaacac atcacggagt ttcaacgtga gcaacaaagg gcagctctta caagacccct
     1141 tcctgaatct gcttcgcaag gagcacgttc cggtttccat ctacctcgtc aacggcatca
     1201 agctgcaggg ccacatcgag tccttcgacc agtacgtcgt cctgcttcgc aacaccgtga
     1261 ctcagatggt ctacaagcac gcgatttcca ccgtcgtgcc gggccgggcg gtcaatttcc
     1321 aggcgcagcc ggaagccccc gagcaatctt gaggcgtcat ccaggccgcg ccagggcggg
     1381 gccttgaacg ccgggcacga atcggccggg ccgccggccc gggccattct cgtgggcgtc
     1441 gacatcggcc agaaggcgtc cttcgacgcc tcgctcgacg aattggcgct gctcgccgaa
     1501 tcggcaggcg acatcgccgt cgggcggatg gtcgcccggc gcaaggcgcc cgatccggcg
     1561 tttttcatcg gctcgggcaa agccgacgag ctgagggagc tggtgcagct gcaccaggcc
     1621 gatgcggtcc tgttcgacca ggccctttcg ccggcgcagc agcgcaacct cgaaaagcac
     1681 ctgggtgtgg ccgtcgccga ccggaccatg ctgatcctcg agatcttcgg cgagcgcgcc
     1741 cgcagtcacg agggcaagct ccaggtcgat ctggcgcgtc tgcaatacct gtcgacacga
     1801 ctggttcggc gctggagtca cctggagcgc cagcgtggcg gcatcggcaa ccgaggtggc
     1861 cccggggagg cacagatcga gctcgaccgg cgcatgatcg gcgagcgcat caagaacatc
     1921 aagaagcagc tcgagaaggt gaagcgccag cgcagcacgc agcggcgcgc ccgcgagcgc
     1981 aacgagactt tccgggtgtc gctggtcgga tacaccaatg ccggcaagtc gacgctcttc
     2041 aacgcactgt cgggtgccga cgcgtacgtt gccgaccagc tgttcgcgac cctcgacacg
     2101 accacccgcc ggctgcgcat ggacgaggtg gccaggggcg tcgcgctgtc cgataccgtg
     2161 ggcttcatcc gcgaattgcc gcactccctc atcgaggcgt tccaggcgac cctgcaggag
     2221 gccgccgagg ccgacctcct gctgcacgtc gtcgattgcg ccaatccgga gttggccgag
     2281 cagatggccg aggttgcccg cgtattgcgc gagatcgacg cggccgacct cccccaggtg
     2341 gtcgtcttca acaaggtgga cagcctcgcc gataccgagc ggccacgcca gcgggtcgat
     2401 gccatggagc
//
LOCUS       DASRWS010000089         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2051763, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000089 DASRWS010000000
VERSION     DASRWS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051763"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFF72_01600"
     CDS             complement(<1..868)
                     /locus_tag="VFF72_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZV91878.1"
                     /translation="MNRDRFLHVEGDRLVDAAGRTVVLRGYNIGGFLNMENFLTGFPS
                     TESLQRSAMLRAMGESRYCLFFDRFLEVFFADDDARYLRSLGMNHVRVPFNYLHFEDD
                     DRPFELKEGGFTLLDRVVDVCERNDLYAILDLHALPGCQNMDWHSNNPTHHAGFWEHR
                     HFQDRAVHLWERLADRYKGRVAIAGYNLMNEPGDATGARIKPFYDRCVAAVRAIDPDH
                     VIFLDGNRYGTEFTPFEGAEVYPNTVYAAHDYKTPGMVFGGPYPGVTRGIHVDRAHVE
                     ETFLKRTEFMRKT"
     gene            complement(865..1866)
                     /locus_tag="VFF72_01605"
     CDS             complement(865..1866)
                     /locus_tag="VFF72_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZV91879.1"
                     /translation="MNAGKALFIGGSGRLSWSCVERARAQGFEVWVLNRGQNTARPLP
                     EGVHALRGNAREPETVRAAVDGLAFDAVVNFVAFTAADVAQDLETFRGRTGQYMFISS
                     ASCYQTPPARLPVLESTPLHNPVWTYSQHKIAAENLLVAAYRKENWPITIVRPSHTYD
                     QGVVPLIGGWTQIARMRAGKEVLVHGDGSSLWTLTHARDFAKGVVGLLANPRAIGDAF
                     HVTSDESPCWDAIVRTLADAAGVRDLRIVHVPSDAIAAADKDWGDALLGDMAHSLFFD
                     NSKLRRLVPDYLCTTSFADGAREMMTWFDAHPERQIVDAAKDRTIDWLIERHRPAKP"
     gene            complement(1886..>2272)
                     /locus_tag="VFF72_01610"
     CDS             complement(1886..>2272)
                     /locus_tag="VFF72_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91880.1"
                     /translation="CGQPGGIFVLTGDVIAFPKQKTAEAEKAQKLLAKVAMAPATQLA
                     FNMKHASIPPRTDVSIAGNPGFDQCVQSAAALYKGGVHVVRNSALILPADQMGSVVDL
                     VSEYFNSDMPTDEALDRFASILTSGK"
BASE COUNT          356 a          712 c          818 g          386 t
ORIGIN      
        1 cggtcttcct catgaactcg gtgcgcttga gaaaggtttc ctcgacgtgg gcgcggtcga
       61 cgtggatacc gcgcgttacg cccgggtacg ggccgccgaa gaccatgccg ggcgtcttgt
      121 agtcgtgcgc ggcatacacc gtgttcgggt agacctcggc gccttcgaag ggcgtgaact
      181 cggtgccgta gcgattgccg tcgaggaaga tgacgtgatc gggatcgatc gcgcgcacgg
      241 cggcgacgca gcggtcgtag aacggcttga ttcgcgcgcc ggtcgcgtcg cctggctcgt
      301 tcatgaggtt gtagcccgcg atcgcgacac ggcccttgta gcggtcggcg agccgttccc
      361 acaggtgcac cgcgcgatcc tggaaatggc ggtgctccca gaagccggca tggtgggtcg
      421 gattgttgct gtgccagtcc atgttctggc agccgggcag ggcgtgcagg tcgagaatgg
      481 cgtagaggtc gttgcgttcg cagacgtcga cgacgcggtc gagcagcgta aagccgccct
      541 ctttcagctc gaagggccgg tcgtcgtcct cgaagtgcag atagttgaac ggcacccgca
      601 catggttcat gccgagtgag cgcaggtaac gcgcgtcgtc gtcggcaaag aagacctcga
      661 gaaagcgatc gaagaagagg cagtatcgcg attcgcccat cgcgcgcagc atggcgctgc
      721 gctgcagcga ctcggtcgaa ggaaatccgg tgaggaagtt ctccatgttg aggaagccgc
      781 cgatgttgta accgcgcagg accaccgtgc ggccggccgc gtccacgagg cggtcgccct
      841 ccacgtgcag gaagcggtcg cggttcatgg cttggcgggc cgatgacgct cgatcagcca
      901 gtcgatcgtc ctgtccttgg cggcgtcgac gatctgccgc tcgggatgcg cgtcgaacca
      961 ggtcatcatc tcgcgtgcgc cgtcggcgaa cgaggtcgtg cacaggtagt cgggcacgag
     1021 ccggcgcagc ttcgagttgt cgaagaagag gctgtgcgcc atgtcgccga ggagggcgtc
     1081 gccccagtcc ttgtcggcgg cggcgatcgc gtccgacggc acgtggacga tgcgcaagtc
     1141 gcgcacgccg gcggcgtcgg cgagcgtgcg cacgatcgcg tcccagcagg gcgattcgtc
     1201 ggacgtgacg tggaaggcat cgccgattgc ccgaggattc gccagcagcc cgaccacgcc
     1261 cttggcgaag tcgcgcgcgt gggtcagcgt ccacagcgac gagccgtcgc cgtgcacgag
     1321 cacttccttg ccggcgcgca tgcgggcgat ctgcgtccag ccgccgatca gcggcacgac
     1381 gccctggtcg taggtgtgcg aaggccgcac gatggtgatc ggccagttct ccttgcggta
     1441 ggctgcgacg agcaggttct cggcggcaat cttgtgctgc gagtaggtcc agaccgggtt
     1501 gtgcagcggc gtcgactcga gcacgggcag ccgggccggc ggcgtctgat agcacgaggc
     1561 cgagctgatg aacatgtact ggccggttcg gccgcggaag gtctcgaggt cctgggcgac
     1621 gtcggcggcg gtgaaggcga cgaagttgac gaccgcgtcg aaggcgagcc cgtcgaccgc
     1681 ggcgcgcacg gtctcgggct cgcgggcatt gccgcgcagc gcgtgcacgc cctcgggcag
     1741 tggccgcgcc gtgttctggc cgcgattgag cacccagacc tcgaagccct gcgcccgcgc
     1801 ccgttccacg caggaccacg agagtcgccc gctgccgccg atgaagagag ccttgcccgc
     1861 gttcatcgtc cgtcgcctcg cctgctcact tgccgctcgt caggatcgac gcgaagcggt
     1921 cgagcgcctc gtcggtcggc atgtcgctgt tgaagtattc gctgaccagg tcgactaccg
     1981 agcccatctg gtcggccggc aggatgagcg ccgagttgcg caccacgtga acgccaccct
     2041 tgtagagcgc cgccgcggac tgcacgcact ggtcgaagcc ggggttgccg gcgatcgaga
     2101 cgtcggttcg cggcggaatc gaggcgtgct tcatgttgaa ggcgagctgc gtggccggcg
     2161 ccatcgccac cttggcgagc agcttctgcg ccttctcggc ctcggcggtc ttttgcttcg
     2221 gaaaggcgat gacgtcgccg gtgagcacga agatgccgcc gggctggccg ca
//
LOCUS       DASRWS010000090         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2052252, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000090 DASRWS010000000
VERSION     DASRWS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052252"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFF72_01615"
     CDS             <1..277
                     /locus_tag="VFF72_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91881.1"
                     /translation="IPMHEKLAELSHRAGESGRAVREREAILALGPVDRAEALYELAA
                     AQHDAGDDVHARTTVLRALEGAPDFEKAQTLLLTLYDARQSGGAKKP"
     gene            274..1110
                     /locus_tag="VFF72_01620"
     CDS             274..1110
                     /locus_tag="VFF72_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZV91882.1"
                     /translation="MTKSILGTAAMLLVVLSTSAGAQRRRTNGYYAQSLGSPDDFYVP
                     PDYHGNPPYDGRFIFARIEYRGFEHWSGREGPGWSHDYPDAEENFMKIVRDITVMHPF
                     VQAGPIVGSAIVALDDPALFKYPVAYMSEPGGWNPNEKEMAALRTYLLKGGFIIFDDF
                     RENWRGDFDWTHLRQIFAHMMPTARWVQLTGNEPIFDSFFKIDLKAAISSTSAYGTEP
                     PTYWGIYEDNDPKKRLMLIANVDNDIGESWQWSASGFVPIAAANETYKLGVNYIVYAL
                     TH"
     gene            1147..2178
                     /locus_tag="VFF72_01625"
     CDS             1147..2178
                     /locus_tag="VFF72_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZV91883.1"
                     /translation="MTSTLPAPTTTATDDIALAERLKIGHDAIVTELHKLIVGQEQVI
                     ELVLLSLFTGGNSLIVGVPGLAKTLLINTISQVLDLKFSRIQFTPDLMPSDITGTDII
                     QDDPATGRRHMVFTPGPIFANIVLADEINRTPPKTQSALLEAMQEHRVTVQGKTFRLE
                     EPFHVFATQNPIELEGTYPLPEAQLDRFMFNIVMDYLPEDQEVQVATSTTSVQNHRFQ
                     HAIGGSDIIAFQQLVRRVPIAEAVARYAVSLARASRPGPKSPDFIRQWVSYGASTRAP
                     QQLILGGKARALMDGRFNVSFDDIRALATPVLRHRVLTNFHAESEGVTPDQIVEKLLN
                     AVAIPRSGL"
     gene            2244..>2789
                     /locus_tag="VFF72_01630"
     CDS             2244..>2789
                     /locus_tag="VFF72_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZV91884.1"
                     /translation="MPTYLDPTVLSRISNLELLARTVVEGFVNGLHRAPHLGSSTDFA
                     EHRPYMPGDDTRRIDWRLFARSDRHFVKEYEADTNMNFTVILDVSPSMRFGASPDGGR
                     VSKLTYGCYVAACLAYFSNLQRDRVGLATIDAGIVDYVPPSAKHLQHILHALDRVESS
                     GRSAAGSPGRATLLQPMRKLAD"
BASE COUNT          524 a          924 c          865 g          476 t
ORIGIN      
        1 cattccgatg cacgagaagc tcgccgagct gtcgcaccgc gcgggtgaat cggggcgagc
       61 cgtgcgcgaa cgcgaagcga ttctggcgct cggaccggtc gatcgcgccg aggcgctgta
      121 cgagctggcg gcggcacagc acgatgccgg tgacgacgtg cacgcgcgca cgaccgtact
      181 gcgcgcgctc gagggcgcgc cggatttcga gaaggcgcaa accctgctgc tgacgctgta
      241 cgacgcgcgt cagagtggag gagcgaagaa gccatgacaa aatcgatact cggcacggca
      301 gcgatgctgc tcgtggtgct ctcgacctcc gcgggtgcgc agcgccgccg aactaacggc
      361 tactacgcgc aatccctcgg cagtcccgac gatttctacg tcccgccgga ctatcacggc
      421 aacccgccgt acgatggccg cttcatcttc gcgcggatcg agtaccgtgg cttcgagcat
      481 tggtcaggcc gggagggacc cggatggtca cacgattatc cggatgccga agagaatttc
      541 atgaagatcg tacgcgacat cacggtgatg catccattcg tgcaggcggg tccgatcgtc
      601 ggcagcgcga tcgttgcgct cgacgacccg gcgctgttca agtatccggt cgcatacatg
      661 tccgagccgg gcggctggaa tccgaacgag aaggagatgg cggccctgcg gacctatctg
      721 ctcaagggcg gattcatcat cttcgacgat ttccgggaga actggcgcgg cgacttcgac
      781 tggacgcacc tgcgccagat cttcgcacac atgatgccta cggcgcggtg ggtgcagctc
      841 acgggcaacg agccgatctt cgattcgttc ttcaagatcg atctgaaggc ggccatttcc
      901 tccacgtctg cctacggaac ggagccgccg acgtactggg gcatctacga agacaacgat
      961 ccgaagaagc gcctgatgct gatcgccaac gtcgacaacg acatcggcga atcatggcag
     1021 tggtccgcct ccgggttcgt accgatcgcc gctgccaacg aaacgtacaa gctgggcgtg
     1081 aactacatcg tctacgcatt gacgcattga gcaggtcgcc actcgcaccc aggacccagg
     1141 acctacatga cctcgacact tcccgcgccc acgacgaccg cgaccgatga catcgcgctg
     1201 gccgagcggc tcaagatcgg ccacgatgcg atcgtcacgg agctgcacaa gctgatcgtc
     1261 gggcaggagc aggtcatcga gctggtgttg ctgtcgctgt tcacgggcgg caacagcttg
     1321 atcgtcgggg tgccgggcct ggcgaagacg ctgctgatca acacgatctc gcaggtgctc
     1381 gatctcaagt tttcgcgcat ccagttcacg cccgacctga tgccgagcga catcaccgga
     1441 accgacatca tccaggacga cccggcgacc ggccggcggc acatggtgtt cacgccaggg
     1501 ccgatcttcg cgaacatcgt cttggccgac gagatcaatc ggacgccccc gaagacgcaa
     1561 tcggcgctgc tcgaggcgat gcaggagcat cgggtcacgg tgcaaggcaa gacgttccgg
     1621 ctcgaggagc catttcacgt gtttgcgacg cagaatccga tcgagctgga gggaacgtac
     1681 ccgctccccg aggcgcagct cgaccggttc atgttcaaca tcgtgatgga ctacctgccg
     1741 gaggaccagg aagtgcaggt ggcgacctcc acgacgtccg tccagaacca tcgcttccag
     1801 cacgcgatcg gcgggagcga catcatagcc ttccagcagc tggtccgccg ggtcccgatc
     1861 gccgaggcag tcgcacgcta tgcggtcagc ctcgcgcggg ccagccgtcc cggcccgaaa
     1921 tcgccggact tcattcgcca gtgggtgtcg tacggtgcca gcacgcgtgc gccgcagcag
     1981 ctcatcctcg gcggcaaggc tcgcgcgctg atggacggcc gcttcaacgt cagcttcgac
     2041 gacattcgcg cgctggccac acccgtgctc cgccaccgcg tgctcacgaa cttccacgcc
     2101 gagtccgagg gcgtgacgcc agaccagatc gtcgagaagt tgttgaacgc cgtggccatt
     2161 ccccgttccg gcttgtagct cgtccaccgg ccggcgtcca tggcaccacg gacgcccgac
     2221 catggaccat cgatcaatca ccgatgccga cctatctcga tcccaccgta ctctcccgca
     2281 tcagcaatct cgaattgctg gcgcgcacgg tcgtcgaggg attcgtcaat gggctgcatc
     2341 gggcgccgca cctcggttcg tccaccgatt tcgccgagca tcggccgtac atgccgggcg
     2401 acgacacccg gcggatcgac tggcggctgt tcgcccgcag tgatcggcac ttcgtcaaag
     2461 agtacgaggc cgacacgaac atgaatttca cggtgatcct cgacgtgtcg ccgtcgatgc
     2521 gattcggtgc gagtcccgac ggtggtcgcg tatcgaagct cacctacggg tgctacgtcg
     2581 ccgcctgcct cgcgtacttc tcgaatctgc agcgcgatcg cgtcgggctg gcgacgatcg
     2641 acgccggcat cgtcgattac gttccgcctt cggccaagca cctgcagcac atcctgcacg
     2701 cactggatcg ggtggaatca tcggggcgca gtgcggccgg ttcgcccggg cgcgcgacgc
     2761 tgcttcagcc catgcgcaag ctcgccgac
//
LOCUS       DASRWS010000091         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_615132, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000091 DASRWS010000000
VERSION     DASRWS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615132"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFF72_01635"
     CDS             <1..213
                     /locus_tag="VFF72_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV91885.1"
                     /translation="QAFLDEQAGQCGYCLSGIILSAKALLDRNPKPSRAQIVEALDRH
                     LCRCGTHQRIVRAVERAAPMIQKGLA"
     gene            210..>2034
                     /locus_tag="VFF72_01640"
     CDS             210..>2034
                     /locus_tag="VFF72_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZV91886.1"
                     /translation="MNAPNLPTSLVDNPRLDRWIHFNPDRTVRVASGKVEIGQGVVTA
                     LSQIAADELDVPLDRVHLLSGDTDHGPDEMYTTSSLSIQMSGSSIRLVCAEVRAKALD
                     RAALRLNCSPDDLTVVDGQFLQNGEATGQDYWTVAGEIDFSQAPTGAGKLKKADAYKI
                     VGRDVPRFDLPAKITGAAYVHDVLPSNVLHARTLRQPNPGATLAALDEAAIKRAAKGE
                     LQIVREANFVAFVSPVESVAQAAAAAAPAHARWDNLRQLTPAHQEAAWLKGQPAEDRQ
                     IGAPPRTSPPKNPAQITVSRPYIAHASMGPSCALAEFRDGRLTVQSHGQGMHPLRKNL
                     AAALKLPIEAITAKHLHGPGCYGHNGADDAALDAALVAMRIPGRTIRLQWRREEEFGF
                     EPVGPAMLVTLHVDLDDRGRPTDWTTEIWSATHVQRPGSGSGYLLATEALSDPPPEVK
                     PTDPTEQRGGGGTRNAVPLYDVPSHRILHHLVQRGPVRTSALRGLGALPNVYAIEMMM
                     DELAARVGEDPVAYRLSILSEPRARRVVERIAAMSHWKARGPTGAGKGLGMAFARYKN
                     MAAYCAVAAAVTVDEVVRVDRVWCVADAGLIVNPNGARNQLE"
BASE COUNT          326 a          721 c          702 g          285 t
ORIGIN      
        1 caggccttcc tcgacgagca ggccggccag tgcggctact gtctgtcggg catcatcctg
       61 tcggccaagg ccctgctcga tcgcaatccc aaaccgtcgc gtgcgcagat cgtcgaggcg
      121 ctcgaccgcc acctctgtcg ctgcggcacc catcaacgca tcgtccgcgc cgtcgagcgc
      181 gccgcgccta tgattcaaaa ggggctcgca tgaacgcgcc gaacctgccg accagcctgg
      241 tcgacaatcc gcggctcgac cgctggatcc atttcaatcc ggaccgcacg gtgcgcgtcg
      301 cctcgggcaa ggtcgagatc ggccagggtg tcgtcacggc gctgagccag atcgccgccg
      361 acgagctcga tgtgccgctc gatcgcgtcc acttgctgtc aggcgacacc gatcacggcc
      421 cggacgagat gtacacgacg tcctcgctgt cgatccagat gtcgggcagc tcgatccgcc
      481 tggtctgcgc cgaggtgcgc gccaaggcgc tcgaccgcgc ggcgctgcgg ctgaactgca
      541 gcccggacga tctcaccgtg gtcgacggcc agttcctgca gaacggcgag gcgaccggtc
      601 aggattactg gacggtcgcg ggcgagatcg acttctcgca ggcaccgacg ggcgccggga
      661 agctgaagaa ggccgacgcc tacaagattg tcggcaggga cgtcccgcga ttcgacctgc
      721 cggcgaagat caccggtgcg gcctacgtgc acgacgtgct gccaagcaac gtgctgcatg
      781 cccgcacgct gcgccagccc aatcccggcg cgaccctagc ggcgctcgac gaggcggcga
      841 tcaaacgcgc ggcaaagggc gagctgcaga tcgtgcgcga ggccaacttc gtcgccttcg
      901 tgagcccggt cgaaagcgtg gcgcaggctg cggcagccgc ggcaccggcg catgcgcgct
      961 gggacaatct tcgccagctc acgccggcgc atcaggaggc ggcatggctc aagggccagc
     1021 cggccgagga tcgccagatc ggtgcgccgc cgcgcacgag cccgccgaag aacccggcgc
     1081 agatcaccgt gtcgcggccc tacatcgcgc acgcgtcgat gggtccgtcc tgcgcgctgg
     1141 ccgagttccg cgacggccgt ctgacggtac agtcgcacgg ccagggcatg catcccttgc
     1201 gaaagaacct cgcggccgcg ctcaagctgc cgatcgaagc gatcacggcg aagcacctgc
     1261 acggccccgg ctgctacggt cataacggcg ccgatgacgc cgcgctcgac gcggcgctgg
     1321 tcgccatgcg cattcccggc cgcaccattc gactgcaatg gcggcgcgag gaggagttcg
     1381 gcttcgagcc ggtcgggccg gcgatgctgg tgaccctgca tgtcgacctc gacgacaggg
     1441 gccggccgac cgactggacg accgagatct ggagcgccac gcacgtgcag cggccgggca
     1501 gcggcagcgg ctacctgctg gcgaccgagg cgctgtccga tccgccaccc gaggtgaagc
     1561 cgaccgatcc gaccgagcag cgcggcggcg gcggcacgcg caatgccgtg ccgctgtacg
     1621 acgtgccgtc ccatcgcatc ctgcatcacc tggtgcagcg cgggccggtg cgcacctcgg
     1681 cgctgcgcgg cctgggtgct ctgcccaacg tctacgcgat cgagatgatg atggacgagc
     1741 tcgccgcgcg cgtcggcgaa gaccccgttg cctatcgcct ctcgatcctg tccgagccgc
     1801 gggcgcggcg ggtggtcgag cgtattgcgg cgatgagcca ctggaaggcg cgtggcccga
     1861 ccggcgcggg caagggcctc ggcatggcgt tcgcgcgtta caagaacatg gccgcctact
     1921 gcgccgtcgc cgcggccgtg acggtcgacg aggtcgttcg cgtcgaccgc gtctggtgcg
     1981 tggccgatgc cgggctgatc gtgaacccca acggcgcgcg caaccagctc gagg
//
LOCUS       DASRWS010000092         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3487126, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000092 DASRWS010000000
VERSION     DASRWS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487126"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..324
                     /gene="gatC"
                     /locus_tag="VFF72_01645"
     CDS             25..324
                     /gene="gatC"
                     /locus_tag="VFF72_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZV91887.1"
                     /translation="MALTLSDVERIAELARLQLDADGSAQMHEQLNGFFRIVELMSQV
                     DTSGVEPLLTPLAAVFDAHLRLRDDVVTEADEREANQRSAPVVADGLYLVPRVIE"
     gene            321..1820
                     /gene="gatA"
                     /locus_tag="VFF72_01650"
     CDS             321..1820
                     /gene="gatA"
                     /locus_tag="VFF72_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZV91888.1"
                     /translation="MSDDLHTLGVAALGRALAERRVSSVEVTRHLLDRLSTYSHLGVS
                     LAEDPGHALASARAADARRASGEAGPLLGVPIGHKDIFVTRDLPTTAGSRMLAGYRSP
                     FDATVVARLAEAGMVTIAKLNCDEFAMGSSNENSAFKVVRNPWDEGRIPGGSSGGSAA
                     AVAARLLPAATGTDTGGSIRQPASFCGITGIKPTYGVCSRYGMIAFASSLDQAGPLAR
                     SAEDCALLLSTMSGFDPRDSTSADRPPEDYVAALARPREGATAAQPLHGLRIGLPREF
                     FPAGLDADVASALRDALAELQRLGASLVDVSLPRTELAIPVYYLIAPAEASSNLSRFD
                     GVRYGHRASRYADLQTLYKTSRSEGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKLRR
                     MIADDFQACFRQCDLIAGPVAPTVAWRLGDKSDDPVANYLADIFTLPASLAGLPGMSV
                     PAGFSREPAAMPIGLQLVANYFDESRLLHAAHAFQRATDWHVRAPAAAP"
     gene            1832..3361
                     /gene="gatB"
                     /locus_tag="VFF72_01655"
     CDS             1832..3361
                     /gene="gatB"
                     /locus_tag="VFF72_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZV91889.1"
                     /translation="MSTSPSIGAALVRGWEVVIGIETHAQLSTRSKIFSGSSTAFGAA
                     PNTQASAVDVALPGTLPVANRGAVERAIRFGLAVGGQVAPISIFARKNYFYPDLPKGY
                     QISQYETPVVQGGTIEFFVGETAHRVQLTRAHLEEDAGKSTHGIAHADADALSDADAQ
                     GALRSALDFGGDWTGIDLNRAGTPLLEIVSEPEMRSSAEAVAYAKALHTLVVWLDVCD
                     GNMQEGSFRCDANVSVRRPGAPFGTRREIKNLNSFRFMQQAIDYEVQWQIDLLEDGGQ
                     VRQATVLFDPAHGETRAMRSKEDAHDYRYFPDPDLPPLAISTAWIDKVRVSMPELPAE
                     LAARLQSQYSLAAHDASMMTQSKSFASAFEAAAQASGQPKLVANWMMGEVSRRMNAAG
                     LQEMPLAAAQLAALVSRIADGTISNSAARRVFEALWDGGEGADVDALIDGLGLRQLRD
                     SDALERIVDEVIAANPKSVEEFRSGKEKAFNALVGQAMKASRGKADPAQINALLRARL
                     G"
     gene            complement(3286..>3820)
                     /locus_tag="VFF72_01660"
     CDS             complement(3286..>3820)
                     /locus_tag="VFF72_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91890.1"
                     /translation="KEREAAAERVAKNDAIRRDRNLIARYPNEAAHNRARAKALDDLR
                     ASVRVSKERIALLNAERKPLLDEAEFYVGKSLPSKLKLALDANDASLDAQKALIQTRK
                     PKACASMRSTTSSWRGCASYGPARLPDRSAPRRGRRRRLRPKEQPPAEGQFQPRRARS
                     SALICAGSALPRLAFIA"
BASE COUNT          578 a         1396 c         1262 g          584 t
ORIGIN      
        1 acccgcgccg ctgtttttca cgcgatggcc ctgacccttt cagacgtcga acgcatcgcc
       61 gagctggcgc gcctgcaact cgatgcggac ggcagcgcgc agatgcacga gcagctcaac
      121 ggcttctttc gcatcgtcga gctgatgagc caggtcgata ccagcggggt cgagccgctc
      181 ctgaccccgc tcgccgccgt cttcgacgcg catctgcggt tgcgcgacga cgtcgtcacc
      241 gaggcagacg agcgggaggc caaccagcgc agcgcgccgg tcgtggccga cggcctctac
      301 ctcgtgccac gggtcatcga gtgagcgacg atctgcacac gctgggcgtc gccgcgctcg
      361 ggcgcgccct ggccgagcgg cgcgtctcga gcgtcgaggt cacgaggcat ctgctcgatc
      421 gcctctcgac gtactcgcac ctcggcgtct cgctggcaga agatccgggc catgccctcg
      481 cgtcggcacg cgccgccgac gcacggcggg ccagcggcga ggccggcccc ctgctcgggg
      541 tgccgatcgg ccacaaggac atcttcgtca cccgcgacct gccgacgacc gccggctcgc
      601 ggatgctcgc gggctaccgc agccccttcg acgcgaccgt cgtcgcccgc ctcgccgaag
      661 ccggtatggt gacgatcgcc aagctcaact gcgacgagtt cgcgatgggc tcgagcaacg
      721 agaactcggc cttcaaggtc gtgcgcaatc cctgggacga aggacggatt cccggcggct
      781 cgtcgggcgg ctcggcggcg gcggtggcgg cgcggctcct tccggccgcc accggcaccg
      841 acaccggcgg ctcgatccgt cagccagcga gcttttgcgg catcaccggc atcaagccga
      901 cgtacggcgt ctgctcgcgc tacggcatga tcgccttcgc gtcgagcctc gaccaggccg
      961 ggcccctggc gcgcagcgcc gaggattgcg cactgctcct ctcgacgatg agcgggttcg
     1021 atccgcgcga ctcgaccagc gccgaccggc cgccggagga ctacgtcgcc gccctcgcca
     1081 ggccccgcga gggtgcgacg gcggcgcagc ccctgcacgg actgcgcatc ggcctgcccc
     1141 gggagttctt ccccgccggc ctcgacgccg atgtcgcttc ggccctgcgc gacgcgctgg
     1201 ccgagttgca gcgcctgggc gcctcgctcg tcgacgtcag cctgccgcgc accgaactcg
     1261 cgatcccggt ctactacctc atcgcgcctg ccgaggcctc gtcgaacctg tcgcgtttcg
     1321 acggcgtccg ctacgggcat cgggcgagcc gctacgccga cctgcaaacg ctctacaaga
     1381 cgagccgcag cgagggcttc ggcgcggagg tgaagcgccg catcatgatc ggcacctacg
     1441 tgctctcgca cggctactac gacgcctact atctgcaggc gcaaaagctg cgccgcatga
     1501 tcgccgacga tttccaggcc tgctttcgcc agtgcgacct gatcgccggg cccgtcgcgc
     1561 ccactgtcgc atggcgcctc ggcgacaaga gcgacgaccc ggtggcgaac tacctggccg
     1621 acatcttcac cttgcccgcc agcctggccg gcctgcccgg catgagcgtc ccggccggtt
     1681 tctcgcgcga acccgcggcg atgccgatcg ggctgcaact cgtcgccaat tacttcgacg
     1741 agtcgcgcct gctgcacgcg gcgcacgcct tccagcgggc gaccgactgg catgtccgcg
     1801 ctcccgcggc cgcgccctga cgagaacccc gatgagcacg tccccctcca tcggcgcggc
     1861 gctcgttcgc ggctgggaag tcgtcatcgg catcgagacg cacgcccagc tgtcgacgcg
     1921 ctcgaagatc ttcagcggct cctcgacggc cttcggcgcc gcgccgaaca cgcaggcgtc
     1981 ggccgtcgac gtggcattgc ccgggacctt gcccgtggcc aaccgcggcg ccgtggagcg
     2041 ggcgatccgc ttcggcctgg cggtgggcgg ccaggtcgcg ccgatctcca tcttcgcgcg
     2101 aaagaactac ttctatcccg atcttcccaa gggctaccag atcagccagt acgagacgcc
     2161 ggtggtccag ggcgggacga tcgaattctt cgtcggcgaa acggcgcatc gcgtccagct
     2221 gacgcgcgcc cacctcgagg aggacgccgg caagtcgacg cacggcatcg ctcacgccga
     2281 cgccgacgcc ctctccgacg ccgatgccca gggggccctc cgcagtgcac tcgacttcgg
     2341 cggcgactgg accggcatcg acctcaaccg cgccggcacg ccgctgctcg agatcgtcag
     2401 cgagccggaa atgcgctcca gcgccgaggc ggtggcgtac gcgaaggcgc tgcacacgct
     2461 cgtcgtctgg ctcgacgtct gcgacggcaa catgcaggaa ggaagctttc gctgcgatgc
     2521 caacgtgtcg gtccgccggc ccggcgcgcc cttcggcacc cgccgcgaaa tcaagaacct
     2581 gaacagcttt cgcttcatgc agcaggcgat cgactacgag gtgcagtggc agatcgacct
     2641 cctcgaagac ggtggccagg tccgacaggc gacggtcctg ttcgatccgg cgcacggcga
     2701 gacgcgagcc atgcgatcca aggaagacgc gcacgactat cgctatttcc ccgacccgga
     2761 tctgcctccg ctggccatct cgaccgcctg gatcgacaag gtcagggtct cgatgcccga
     2821 gcttccggcc gagctcgccg cgcgactgca aagccagtac tcgctcgccg ctcacgacgc
     2881 gtcgatgatg acgcaaagca aaagcttcgc gtccgcattc gaagccgccg cacaggccag
     2941 cggtcaaccc aagttggtcg cgaactggat gatgggcgag gtgtcgcgcc gcatgaatgc
     3001 ggccggactg caggagatgc cgctggccgc tgcccaactc gccgcgctgg tctcgcgcat
     3061 cgccgacggc acgatctcga acagcgccgc gcgccgggtc ttcgaggcac tctgggacgg
     3121 cggggaaggc gccgacgtcg atgccctcat cgacggcctc ggcctgcgcc agctgcgtga
     3181 cagcgatgca ctcgagcgca tcgtcgacga agtgatcgcc gcgaacccga agtcggtgga
     3241 agagtttcgc tcgggcaagg aaaaggcctt caacgcgctg gtcggtcagg cgatgaaggc
     3301 cagtcgcggc aaggccgacc cggcgcagat caacgcgctg ctgcgcgcac gcctgggctg
     3361 aaactgtccc tcagcgggtg gctgttcttt cggccggagc cgccgccgcc ggccccggcg
     3421 gggggccgag cgatccggga ggcgcgccgg cccatagctt gcgcagccgc gccagctcga
     3481 cgtcgtagag cgcattgatg cgcacgcttt cggcttcctg gtttggatca gcgccttttg
     3541 cgcatcgagc gacgcgtcgt tggcgtcgag ggccagcttc agcttgctcg gcagcgactt
     3601 gccgacgtag aactccgcct cgtcgagcag cggcttgcgc tcggcgttga gaagggcgat
     3661 ccgctccttc gagacgcgta ccgaggcgcg caggtcgtcg agggccttgg cgcgcgccct
     3721 gttgtgcgcc gcttcgttcg gatagcgggc gatgaggttg cggtcacgcc ggatcgcgtc
     3781 gttcttggcc acccgttccg cggccgcctc gcgctccttc
//
LOCUS       DASRWS010000093         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2053430, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000093 DASRWS010000000
VERSION     DASRWS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053430"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..666)
                     /gene="pal"
                     /locus_tag="VFF72_01665"
     CDS             complement(127..666)
                     /gene="pal"
                     /locus_tag="VFF72_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZV91891.1"
                     /translation="MKSIQRHLLSALAVASLAACSSVPLDSTPVETRTGSAITNGSGS
                     GSGASSSQTKVANVDVTNNNGASSMSNIPRIVYFDFDSYVVGDQYRPVIEANAKELVR
                     NPREHMSVQGNTDDRGSSEYNLALGQRRADAVVKSLELLGAQPGQLEAVSFGKERPAV
                     QGDNEEAWAKNRRVELASK"
     gene            complement(712..1992)
                     /gene="tolB"
                     /locus_tag="VFF72_01670"
     CDS             complement(712..1992)
                     /gene="tolB"
                     /locus_tag="VFF72_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HZV91892.1"
                     /translation="MFFDRRRFCAAGAALFTAPVAFAQFKVEISGVGATQLPIAVVRF
                     RNEDAAGANGPSAIVRADLERSGAFRIVDASATLDETSQPAMGEWRSRSADALVAGSV
                     ARLADGRFDIRFKLWDVVKGSDLGGQTAAVDPGDLRLAAHRIADFVYQKLTGERGVFS
                     TRIAYVTRARGHHTLRVADADGENGQVALNSSQPIISPAWSPDGKELAYVSFEQQKAV
                     VFVHTVATGQRRAIADFRGSNSAPAWSPDGSYLAVTLSRDGGSQLFFIGKDGSNPRRI
                     TTSQAIDTEAQFSADGKYLYFTSDRGGSPQIYRMAMPPGRVERVTFTGGYNISPAVSP
                     DGRTLAYIARSGNAFRLSILDLSTPGAQPVQITDTSDDDHPSFAPNGRLLIYATKVQG
                     RSVLMTTTLDGKIKARLPSATADLREPVWGPFGR"
     gene            complement(2025..2819)
                     /locus_tag="VFF72_01675"
     CDS             complement(2025..2819)
                     /locus_tag="VFF72_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZV91893.1"
                     /translation="MPSPTLSVILITKNESANIDACLASVDFADEWIVVDSGSTDDTR
                     ERAARHGASVVVTDDWPGFGAQKQRALDRATGRWVLALDADERVTPELAASIREVVRA
                     DSAAPSAFELSRLSSFCGRWIRHGDWYPDRVLRLFRRGEARFSNDRVHERVIAGGQVG
                     RLRGELLHLTMPTLADALAKMNRYSSEGAAQAAASGKRTGAVAALAHAAWAFLRGYGL
                     RRGFLDGAAGFTVAAYVAEGTFWRYLKTAELARPLPRGDGPPAPSK"
     gene            2909..4642
                     /gene="msbA"
                     /locus_tag="VFF72_01680"
     CDS             2909..4642
                     /gene="msbA"
                     /locus_tag="VFF72_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HZV91894.1"
                     /translation="MTTPSSPLRERIRRILPFFANSRRGFVIAFFGLVIGAFTELLIP
                     MLLKWLLDNGFKLGGVPLWTIPTAVIGLTAIRGASGFVAQYGMAWAANRGVMDMRAQM
                     FDRVLDAEPALFGKQAASNLINTLTFEVQNGATQLVYAVQYLVRDSLTLVVLLGYLIY
                     LNWQLTLIVAVLMPAVAQVMRFYSKRLHRFTVESQKATDELAYVVEENVLAWRSVRLH
                     AAQPAQGGRFRAASDRLRRVSLKAVVAAATMTPLTQVLASCALAAVITAAIWQSSRDA
                     TTVGAFVAFITALLQLISPMKHLSEISAPVARGLAALDRGVRLIDETPAETGGSFDPG
                     RAKGRIELRGVSMAYRQEQAALRDVNLQIRPGEAVALVGPSGAGKTTIINLLPRFIDP
                     TGGQILLDGVPLGEWNVAALRRQFALVSQDVSLFNDSVAANVALGAAIDRPRVVEALR
                     AAHLLDFAQALPNGIDTLVGHNAGELSGGQRQRLAIARAIYKDAPILILDEATSALDS
                     ESERHVQDALETLMRGRTSLVIAHRLSTVEHADRIVTLEAGRVVEQATHAELLAAGGL
                     YARLHALQFRS"
BASE COUNT          687 a         1612 c         1640 g          742 t
ORIGIN      
        1 cggcgcgcgc ctgcggcgcg aacgccgacg ccatggcgag cagcactgcc gccgccgcgc
       61 cgtggcgcgc aagcgcaatg cggcgggcgg gcctgcccgc ctccggaggc agcgatcgat
      121 ccgctctcac ttgctggcca gctcgacgcg gcggttcttg gcccacgctt cctcgttgtc
      181 gccctgcacg gccggccgct ccttgccgaa gctcaccgcc tcgagctggc ccggctgggc
      241 gccgagcaac tcgagcgact tcaccacggc gtcggcgcgg cgctggccga gcgccaggtt
      301 gtattcgctg ctgccgcggt catcggtatt gccctgcacc gacatgtgct cgcgcgggtt
      361 cctgaccagc tccttggcgt tggcctcgat gaccgggcgg tactgatcgc cgacgacgta
      421 gctgtcgaag tcgaagtaga cgatgcgcgg aatgttcgac atcgacgagg cgccgttgtt
      481 gttggtgacg tcgacgttgg cgaccttggt ctgcgagctg gaggcaccgc tgccgctgcc
      541 gctgccgttg gtgatggccg agccggtgcg tgtctcgacc ggcgtgctgt cgagcgggac
      601 gctcgagcaa gccgcgagcg aggcgaccgc cagggcactc aggaggtggc gctggataga
      661 tttcatggtg gtttcctttt tccctcaaag tgaacgccaa agccggcgcg gctatcgccc
      721 gaaggggccc caaaccggct cgcgcaaatc ggcggtcgcc gacggcagtc gagccttgat
      781 cttgccatcg agcgtggtcg tcatcaacac gcttcgaccc tgcaccttcg tcgcgtagat
      841 gagcaggcgg ccgttcggcg cgaagctcgg atggtcgtcg tcggaggtgt cggtgatctg
      901 caccggctgg gcgccgggtg tcgacaggtc gaggatggag aggcgaaacg cgttgccgct
      961 gcgcgcgatg taggcgagcg tcctgccgtc ggggctgacc gccgggctga tgttgtagcc
     1021 gccggtgaag gtgacgcgct cgacccggcc cggaggcatc gccatccggt agatctgcgg
     1081 gctgccgccg cggtcgctcg tgaaatagag gtacttgccg tcggcggaga actgcgcctc
     1141 ggtgtcgatc gcctggctgg tcgtgatgcg gcgcgggttg ctgccgtcct tgccgatgaa
     1201 gaaaagctgc gagcccccgt cgcgcgagag cgtcaccgcg agatagctgc cgtcgggcga
     1261 ccaggccggc gcgctgttgc tgccgcggaa atcggcgatc gcccggcgct ggccggtcgc
     1321 caccgtgtgc acgaagacga ccgccttctg ctgctcgaac gacacgtagg caagctcctt
     1381 gccgtcgggc gaccaggccg gcgagatgat cggctggctg ctgttgagcg ccacctgccc
     1441 gttctcgccg tcagcgtcgg ccacgcgcag ggtgtggtgg ccgcgcgccc gtgtcacgta
     1501 cgcgatgcgg gtcgagaaga cgccgcgctc gccggtgagc ttctggtaga cgaagtcggc
     1561 gatgcgatgc gccgccaggc gcaggtcgcc cggatcgacg gcggcagtct ggccgccgag
     1621 atcgctgccc ttgacgacgt cccagagctt gaagcggatg tcgaagcggc cgtcggcgag
     1681 gcgggcgacc gagccggcga cgagcgcgtc ggccgaccgc gagcgccatt cgcccatcgc
     1741 cggctggctc gtttcatcga gcgtggcgct ggcatcgacg atgcgaaagg cgccactgcg
     1801 ctcgagatcg gcacggacga tcgccgacgg cccgttggcg ccggcggcgt cttcgttgcg
     1861 aaagcgcacg acggcgatcg gcagctgcgt tgcgccgacg ccggagatct cgaccttgaa
     1921 ctgggcgaag gcgacgggcg ccgtgaagag ggcggcaccg gccgcgcaaa agcgtcgacg
     1981 atcgaagaac atggatgggc ctgatcccga aaagggcttt tggttcattt ggaaggcgcg
     2041 ggcggtccat cgccccttgg caaagggcgt gcgagctcgg cggtcttgag gtagcgccag
     2101 aacgtccctt cggccacgta ggcggccacc gtgaagccgg cggcgccgtc gagaaagccg
     2161 cggcgcaggc cgtaaccgcg caggaaggcc caggcggcgt gggcgagcgc cgccaccgcg
     2221 ccggtccgct ttcccgaggc ggcggcctgc gccgcgccct cgctgctgta gcgattcatc
     2281 ttcgccagcg cgtcggccag agtcggcatc gtgagatgga gcagttcgcc gcgcagccgc
     2341 ccgacctgcc cgccggcgat cacgcgctca tggacgcggt cgttcgaaaa tcgcgcctcg
     2401 ccccgacgga acagccgcag gacccggtcg ggataccagt cgccgtggcg gatccagcgt
     2461 ccgcaaaagc tcgacaggcg cgacagctcg aacgccgacg gcgctgccga atcggcacga
     2521 acgacttcgc ggatgctcgc cgccagctcg ggcgtgaccc gctcgtcggc atcgagagcc
     2581 agcacccagc ggccggtggc ccggtcgagc gcacgctgct tctgggcgcc gaagcccggc
     2641 cagtcgtcgg tgacgacgac gctggcgcca tgtcgcgccg cccgctcgcg ggtgtcgtcg
     2701 gtgctgcccg agtcgacgac gatccattcg tcggcgaagt ccaccgaggc cagacaggca
     2761 tcgatgttgg ccgattcgtt cttcgtgatg agaatgaccg aaagcgtggg tgagggcatg
     2821 cgccgattcg tggctggccc ggattgtagg cagcggcaac caccagggcc gattcgcgcc
     2881 ggggaaccgg cgccgataat gccccgccat gacgactcca tcgtcgccgc tgcgcgagcg
     2941 catccgccgc atccttccgt tcttcgcgaa cagccggcgc ggcttcgtga tcgccttctt
     3001 cgggctggtg atcggcgcct tcaccgagct gctgatcccg atgctgctca agtggctgct
     3061 cgacaacggc ttcaagctgg gtggcgtgcc gttgtggacg atcccgaccg cggtgatcgg
     3121 cctgaccgcg atccgcggcg cctcgggctt cgtcgcccag tacgggatgg cctgggccgc
     3181 caatcgcggc gtcatggaca tgcgcgcgca gatgttcgac cgggttctcg acgccgagcc
     3241 cgcgttgttc ggcaagcagg ctgcgtccaa cctgatcaac accctcacct tcgaggtgca
     3301 gaacggcgcc acccagctcg tctacgccgt gcagtacctg gtccgcgatt cgctgacgct
     3361 ggtcgtcctg ctcggctatc tcatctatct gaactggcag ctcacgctga tcgtcgccgt
     3421 gctgatgccc gcggtggcgc aggtgatgcg cttctacagc aagcggctgc accgcttcac
     3481 ggtcgagtcg cagaaggcca ccgacgagct cgcctacgtg gtcgaggaaa acgtcctcgc
     3541 ctggcgcagc gttcgcctgc acgccgcgca accggcgcag ggcggccggt ttcgcgccgc
     3601 cagcgatcgg ctgcgccgcg tctcgctgaa ggcggtcgtc gccgccgcga cgatgacgcc
     3661 cctcacgcag gtgctcgcgt cgtgcgccct ggcggcggtg atcacggcgg cgatctggca
     3721 gagctcgcgc gacgcgacga cggtcggcgc gttcgtcgcc ttcatcaccg cgttgctgca
     3781 gctgatctcg ccgatgaagc acctttcgga gatctcggcg ccggtcgcgc gcggcctggc
     3841 ggcgctcgac cgcggcgtca ggctgatcga cgagacgccg gccgaaaccg gcggcagctt
     3901 cgacccgggc cgggccaagg gccggatcga gctgcgcggc gtgtccatgg cgtaccgcca
     3961 ggaacaggcg gcgctgcgcg acgtcaacct gcagatccgg ccgggcgagg ccgtcgcgct
     4021 ggtcggcccg tcgggtgccg gcaagacgac catcatcaac ctcctgccgc gcttcatcga
     4081 cccgaccggc gggcagatcc ttctcgatgg cgtgccgctt ggcgagtgga acgtggcggc
     4141 gctgcgccgc cagttcgcgc tggtgagcca agacgtttcg ctcttcaacg acagcgtcgc
     4201 cgccaacgtc gccctgggcg cggcgatcga tcggccgcgc gtcgtcgagg cgctgcgcgc
     4261 cgcccatctg ctcgatttcg cacaggcgct gccaaacggc atcgacacgc tggtcggcca
     4321 caacgccggc gagctgtccg gcggccagcg ccagcgtctg gcgatcgccc gtgcgatcta
     4381 caaggacgcg cccatcctca tcctcgacga ggcgacctcg gcgctcgact ccgaatcgga
     4441 gcgccacgtc caggacgcgc tcgagacgct gatgcgcgga cgaacgagcc tggtcatcgc
     4501 ccaccgactg tccacggtcg agcacgccga ccggatcgtc accctcgaag caggccgggt
     4561 cgtcgagcag gcgacgcatg ccgagctgct tgccgccggc ggcctctatg cccgcctgca
     4621 cgcccttcaa ttccggagtt gacgacatga gcgcagcgat cagccgctat cccgttcccg
     4681 a
//
LOCUS       DASRWS010000094         6353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2337741, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000094 DASRWS010000000
VERSION     DASRWS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337741"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFF72_01685"
     CDS             <1..567
                     /locus_tag="VFF72_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZV91895.1"
                     /translation="TVLVGVNDVIAQYQQYPSIPEPTLIANLEAAGALVGSQVNRLAD
                     AGAKVIVSTIPDIGYSPFALAEKSAHIDTDRQQLIMRLVARFNAALRSSLDNDGHRIG
                     LVTDDQQVDSSVQYVGSDGYIDVTDPACDLNQSLLTPPSILDCTDLTLVPNATSTNYL
                     WADDRHLSYGGQINLGNLALSRAQNNPF"
     gene            complement(598..897)
                     /locus_tag="VFF72_01690"
     CDS             complement(598..897)
                     /locus_tag="VFF72_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZV91896.1"
                     /translation="MSIPSAGIDAWIGYAAAMLTTSSFFPQAWLIWRSRDVSGISLGM
                     YTAFTVGVGLWLAYGIRMGDWPIIVANAVTLAQAVAILTMKIVLDRRRLRLRQRR"
     gene            complement(894..2345)
                     /gene="dacB"
                     /locus_tag="VFF72_01695"
     CDS             complement(894..2345)
                     /gene="dacB"
                     /locus_tag="VFF72_01695"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZV91897.1"
                     /translation="MGAFAALLLAAAALCEPAFAARRGASAAAPAGLPPGVAAALQRA
                     RVPASSMVAWVEEAGTTTPRLAWQDERPANPASLMKLVTTFAGLDLLGPAFTWSTPVW
                     LDGRLDNGTLDGDLVIKGYGDPKLTLERIWLLLRRVRQAGVRDIRGAIVVDRSAFAPG
                     NPDPADFDGEPLRPYNAGADALLLDFRSIMLTFTPDPVHGVATVSMDPPLAGVRVDAT
                     VPLTGGACDDWRGTLRAAFADPARIAFDGAFQASCGEKVWPVAYVDPASYSARALVGM
                     WLEAGGRLGHGVHEGSAPATPPSFEMRSPTLTEIVRDINKLSNNVMAQQLFLTLGATQ
                     GGSGTPEAARYVVGRWLRDRVGADASAGTVIVNGSGLSRDSRLSALCLGSLLQSAWSS
                     PVMSELMSSLPVAGVDGTMRKARGAPGHAHLKTGSLRDVVGIAGYVLADSGRRYVVVA
                     IVNDANADAARPALDALVDWVADDRATRSMPRP"
     gene            2584..3276
                     /gene="tsaB"
                     /locus_tag="VFF72_01700"
     CDS             2584..3276
                     /gene="tsaB"
                     /locus_tag="VFF72_01700"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZV91898.1"
                     /translation="MTALLALDTGTERMIVAVGVDDRVGIHEGPGGAKASTLLIGTIM
                     RLLDEAAIGLRDLDAIAFARGPGAFTGLRTACSVTQGLAFGVDKPVLPIDSLLVVAEN
                     ARDGARACRVWATIDARMDQIYAAEYEFAAGAWLTREAPMLTDCEELHRRWQSDPPER
                     VAGDALTVFGDRLQTGSAMRQPKAATSGRDLLALARGAWARGEGVDAALALPLYVRDK
                     VALTTLEREARR"
     gene            3273..3791
                     /gene="rimI"
                     /locus_tag="VFF72_01705"
     CDS             3273..3791
                     /gene="rimI"
                     /locus_tag="VFF72_01705"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZV91899.1"
                     /translation="MSGEFAQRAVRPGDATLRAMTVSSLDGVVELETNVYPFPWSRGN
                     FVDSLAAGYTAWTLNGPGGELIGYCIAMRGVDEMHLLNITIAPPARRRGHARRLLAEL
                     VRHCRHEGAHRLWLEVREGNAVARATYLRLGFAEVGTRRAYYPAPAGRREDAVVMGLD
                     LNDDAGAADALD"
     gene            3778..4593
                     /locus_tag="VFF72_01710"
     CDS             3778..4593
                     /locus_tag="VFF72_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZV91900.1"
                     /translation="MRWTERQLAMLREMGVRVWARDGGGAAGDETAEVATVDAASSSP
                     HRRDASSTASTASTAPIAAATSLPARERDDADRSPPSIDPGGERPRPSRSTPTPQGTS
                     PLGAAGPMAGISLAPADWLIVVEAASAGASQAAARPPGPTRPAAAMSADEERLLAAML
                     RAVRISFDAPTRAGRAERVGVDAGLRDRIADVLEAVRPQVVLALGRAAAEALVGADEP
                     LGRLRGRPHALGDVGVIVTFAPSYLLRHPAEKAKAWADLCLAVRASEAVRANG"
     gene            complement(4644..6038)
                     /gene="glmU"
                     /locus_tag="VFF72_01715"
     CDS             complement(4644..6038)
                     /gene="glmU"
                     /locus_tag="VFF72_01715"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZV91901.1"
                     /translation="MALNVVIMAAGRGTRMRSARPKVLNRLAGRALLQHVLDAVEPLG
                     ASRVVVVTGHGASDVEAALAAPGRTFVRQEPQLGTGHAVQQALPSLDADGTTLILNGD
                     VPLIEPATLRALVAASAGDRLALLTVRLADPSGYGRVVRRVATNGAIESIVEEKDASE
                     EQRRIDEIYTGAMAAPTALLRRWLGALRNDNAAGEYYLTDVVRLAVAGSTPVAAVVAS
                     AEAEVLGVNSAAQLAQLERLFQARAAARLMAEGVRIADPARFDLRGTLRCGSDVEIDI
                     DCIFEGEVELGEGVRVGAFCIVRNARIAAGATIRPFSHIDGESLGASVGAGASVGPFA
                     RLRGGAELGPDVHIGNFVEVKNSTLGRGAKANHLAYLGDASVGERVNFGAGGITANYD
                     GANKHRTTIGAGAHIGSNSVLVAPVTVGEGATVGAGSVIARDAPAGQLTVARSRQESL
                     AGWTRPKKKSDPIA"
     gene            complement(6131..>6353)
                     /locus_tag="VFF72_01720"
     CDS             complement(6131..>6353)
                     /locus_tag="VFF72_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZV91902.1"
                     /translation="GPGPIDDFPYVEREVGILIRDKQGGQALYEAHATNSGSSLGPLE
                     VLPAMFAAAMRDFPHGNATHPHRVRIPAH"
BASE COUNT          899 a         2287 c         2290 g          877 t
ORIGIN      
        1 accgtgctgg tcggcgtcaa cgacgtcatc gcccagtatc agcagtaccc gtcgatcccc
       61 gagccgaccc tgatcgccaa tctcgaggcc gccggcgcgc tggtcggcag ccaggtcaac
      121 cggctggccg atgccggcgc aaaggtcatc gtctcgacga ttcccgacat cggctattcg
      181 ccgttcgcgc tcgccgagaa atcggcgcac atcgataccg accggcagca gctgatcatg
      241 cgtctcgtcg cccggttcaa cgcggcgctt cgttccagcc tcgacaacga cggccaccgc
      301 atcggcctgg tcaccgacga ccagcaggtc gattcgtcgg tgcagtacgt cggctccgac
      361 ggctacatcg acgtcaccga tccggcatgc gacctgaacc agtcgctgtt gacgccgccc
      421 tcgatcctcg actgcaccga cctgacgctg gtgcccaacg cgacgtcgac gaactatctc
      481 tgggccgacg accggcacct gtcttacggc ggccagatca acctcggcaa tctcgccctc
      541 agccgggccc agaacaaccc gttctgaggc ggcctgaaga cgcgcgcggc gcgagcgtca
      601 gcgccgctgc cggagccgga gccggcgccg gtcgaggacg atcttcatcg tcaggatcgc
      661 caccgcctgc gccagtgtca cggcgttggc gacgatgatc ggccagtcgc ccatgcggat
      721 tccgtaggcg agccacaggc cgacgccgac ggtgaacgcg gtgtacatgc cgagcgagat
      781 gccgctcaca tcacggctgc gccagatgag ccaggcctgc gggaagaacg agctcgtcgt
      841 cagcatcgcc gccgcatagc cgatccaggc gtcgatgcca gccgaaggga tgctcacggc
      901 cgcggcatcg accgggtggc ccgatcgtcg gcaacccagt cgacgagcgc atcgagcgcc
      961 ggccgcgcgg cgtcggcgtt ggcgtcgttg acgatggcga cgacgacgta gcggcgtccc
     1021 gaatcggcca ggacgtagcc ggcgatcccg accacgtcgc gcagcgaccc ggtcttcagg
     1081 tgcgcatgac ccggcgcgcc ccgggccttg cgcatcgtgc catcgacgcc ggccaccggc
     1141 agcgagctca tcagctcgga catcaccggg ctcgaccacg ccgactgcag caggctgccc
     1201 aggcacagcg cgctcagccg cgagtcgcgc gacagtccgg agccgttgac gatgacggtg
     1261 ccggcggagg cgtcggcgcc gacccggtcg cgcagccagc ggccgacgac atatcgcgcg
     1321 gcttcgggcg tgcccgaccc gccctgcgtc gcgcccagcg tgaggaagag ctgttgcgcc
     1381 atcacgttgt tgctgagctt gttgatgtcg cggacgattt cggtcagcgt cggcgagcgc
     1441 atctcgaagc tcggcggcgt cgccggggcg ctgccttcgt gaaccccgtg gccgaggcgg
     1501 ccacccgcct cgagccacat gccgaccagc gcgcgtgcgc tgtagctggc cgggtcgacg
     1561 taggcgacag gccacacctt ctcgccgcac gacgcctgga aggcaccgtc gaaggcgatt
     1621 cgcgccgggt cggcgaaggc cgcgcgcagg gtgccgcgcc agtcgtcgca ggcgccgccg
     1681 gtcaacggca cggtcgcatc gactcgcacg ccggccagcg gcggatccat gctcacggtg
     1741 gcgacgccgt gcaccggatc gggcgtgaag gtcagcatga tcgagcggaa gtcgaggagc
     1801 agcgcatcgg cgcccgcgtt gtaaggacgc agcggctcgc cgtcgaagtc ggccgggtcg
     1861 ggattgccgg gcgcgaaggc gctgcggtcg acaacgatgg cgccgcggat gtcgcgaacg
     1921 cccgcctggc ggacccggcg caaaagcagc cagatccgct cgagcgtcag cttggggtcg
     1981 ccgtagccct tgatgacgag atcgccgtcg agcgtgccgt tgtcgagccg gccgtcgagc
     2041 cagaccggcg tcgaccacgt gaacgccggc cccagcaggt cgaggccggc gaaggtcgtc
     2101 accagcttca tcaacgaggc cgggttcgca gggcgttcgt cctgccacgc caggcgcggc
     2161 gtcgtcgtgc ccgcttcctc gacccaggcc accatcgacg acgccggcac cctggcgcgt
     2221 tgcagcgccg cggccacgcc tggcggcagg cccgccggcg cggcggccga tgcgccgcgt
     2281 cgagccgcga aggccggctc gcagagcgcg gcagcggcga gcagcagcgc ggcgaaggca
     2341 cccatcgcga tgctcgaaga tcgacgcgtg ggcagactca tgcgggcgat gatcgcacgc
     2401 gacgatggcg cgcctcggcg aagaggcgag gccgcccgcg atggcgatga gcgcgtcacg
     2461 gccgccgcta aactggcaac gatgatgccg cgttcgcccc ccccggcggt ccgtcccggc
     2521 gagtcgcccg gcgcggtggc cgcatctgca atccacgccg cacatctgcc ttcgcctcga
     2581 cgcatgacgg ccctgcttgc cctcgacacc ggcaccgagc ggatgatcgt cgcggtgggc
     2641 gtggacgatc gcgtcggcat ccacgagggg ccgggcggcg cgaaggcttc gaccctcctg
     2701 atcggcacga tcatgcggtt gctggacgaa gcggcgatcg gtctgcgcga tctcgacgcc
     2761 attgcgttcg cgcgcggccc gggcgccttc acggggctgc gcacggcgtg ctcggtcacg
     2821 cagggcctgg ccttcggcgt cgacaagccg gtgctgccga tcgacagcct tctcgtcgtc
     2881 gccgagaacg cgcgcgacgg cgcccgggcg tgccgggtct gggcgacgat cgacgcccgc
     2941 atggaccaga tctacgctgc cgagtacgag ttcgccgccg gcgcgtggct gacgcgcgaa
     3001 gcgccgatgc tgaccgactg cgaagagctg caccggcgct ggcaaagcga cccgccggag
     3061 cgggtcgccg gcgatgcgct gacggtcttc ggcgatcgcc tgcagaccgg cagcgcgatg
     3121 cgccagccga aggcggccac gagcgggcgc gacctgctcg cgctggcacg cggcgcgtgg
     3181 gcacgcggcg agggcgtcga cgcggccctg gcgctgccgc tctacgtgcg tgacaaggtc
     3241 gccctgacca cgctcgagcg cgaggcgcgg cgatgagcgg cgagttcgcg cagcgcgccg
     3301 ttcgcccggg cgatgcgacg ctgcgggcga tgaccgtgtc gtcgctcgac ggtgtcgtcg
     3361 agctggagac gaacgtctat ccgtttccct ggagccgcgg caatttcgtc gattccctgg
     3421 ccgcgggcta cacggcgtgg accctcaacg gcccgggtgg cgagctgatc ggctattgca
     3481 tcgcgatgcg cggcgtcgac gagatgcatc tgctcaacat cacgatcgcg ccgccggcgc
     3541 gtcgacgcgg tcatgcgcgc cggctgctcg ccgagctggt gcggcactgc cggcacgaag
     3601 gcgcgcaccg gctgtggctg gaagtgcgcg agggcaacgc cgtcgcccgc gcgacctacc
     3661 tgcgcctcgg cttcgccgag gtcggaacac gcagggccta ctacccggcc cccgcgggac
     3721 ggcgcgaaga cgcggtcgtc atgggcctcg atctcaacga cgatgcgggt gcggccgatg
     3781 cgctggactg agcgacagct cgcgatgctg cgcgagatgg gcgtgcgcgt ctgggcgcgc
     3841 gacggcggcg gcgcggccgg cgacgagacg gcagaggtcg cgaccgtcga tgcggcgtcg
     3901 tcgtcgccgc atcgacgcga cgcttcgtcg accgcgtcca ccgcatcgac cgcgccgatc
     3961 gctgccgcga cgtcgctgcc ggcgcgcgag cgcgacgatg ccgatcgatc gccgccgtcg
     4021 atcgatccgg ggggcgagcg accgaggcct tcgcgcagca cgccgactcc ccaggggacg
     4081 tcgccgctcg gggcagccgg tccgatggcc ggaatctcgc tcgcgccggc cgactggctg
     4141 atcgtggtcg aagccgcttc ggccggcgca tcgcaggccg ccgcccgccc tcccggaccg
     4201 acgcgacccg ccgcggcgat gtcggccgac gaggagaggc tgctcgcggc gatgctgcgc
     4261 gccgtgcgca tctcgttcga cgcgccgacg cgggccggcc gggccgaacg ggtcggcgtc
     4321 gacgccggcc tgcgcgatcg gatcgccgac gtactcgagg cggtgcggcc gcaggtcgtg
     4381 ctggccctgg gtcgggctgc cgccgaagcg ctggtcggcg ccgacgagcc gctcggccgc
     4441 ttgcgcggtc gcccccacgc gctcggcgat gtcggcgtga tcgtcacttt cgcgccgagc
     4501 tacctgctgc ggcatccggc cgaaaaggcc aaggcgtggg ccgatctttg cctggcggta
     4561 cgcgccagcg aagcagttcg ggcgaacggc tgacggcgcg ggacagcggg ctggtgggct
     4621 ggctcgcggc accgcgccgg cggtcacgcg atcggatcgc tctttttctt cggccgggtc
     4681 cagccggcga ggctttcctg gcggctgcgc gccaccgtca gctgtccggc cggcgcgtcg
     4741 cgggcgatga ccgagccggc accgacggtc gcgccttcgc ccaccgtgac cggcgcgacc
     4801 aggacgctgt tgctgccgat gtgcgcgccg gcaccgatcg tcgtccggtg cttgttcgca
     4861 ccgtcgtagt tcgcggtgat gccgccggcg ccgaaattca cgcgctcgcc gacgctggcg
     4921 tcgccgagat aggcgagatg gttggccttg gcgccgcggc cgagggtcga gttcttgacc
     4981 tcgacgaaat tgccgatgtg cacgtccggg ccgagctcgg cgccgccgcg caggcgcgcg
     5041 aacgggccga cgctggcacc ggcgccgacg ctggcgccca acgactcgcc gtcgatgtgc
     5101 gagaacggcc ggatcgtcgc cccggcagcg atgcgcgcat tgcgaacgat gcagaacgcg
     5161 ccgacgcgca ctccttcgcc gagctcgacc tcgccttcga agatgcagtc gatgtcgatc
     5221 tcgacgtcgc tgccgcagcg cagcgtgccg cgaaggtcga agcgtgccgg gtcggcgatt
     5281 cgcacgccct cggccatcag ccgcgcggcg gcgcgtgcct ggaaaagccg ttcgagctgc
     5341 gccagctgcg cggcgctgtt gacgccgagc acttccgcct cggccgaggc gacgacggcc
     5401 gcgaccggcg tcgagccggc cacggcaagt cgcaccacgt cggtgaggta gtactcaccg
     5461 gcggcgttgt cgttgcgcag cgcgccgagc cagcgcctga gcaatgccgt cggcgcggcc
     5521 atggcgccgg tgtagatctc gtcgatgcgg cgctgctctt cgctggcgtc cttttcctcg
     5581 acgatcgatt cgatcgcgcc gttcgtggcg acgcgccgga cgacccggcc gtagcccgaa
     5641 gggtcggcga gccggactgt cagcaacgcg agccggtcgc cggcgctcgc cgcaacgagc
     5701 gcgcgcaggg tcgccggctc gatcagcggc acgtcgccgt tgaggatcag cgtcgttccg
     5761 tcggcgtcga gcgagggcag cgcctgctgg accgcgtggc cggtgcccag ttgcggctcc
     5821 tgccgcacga aggtgcggcc gggcgccgcc agggccgcct cgacatcgct ggccccgtgc
     5881 ccggtgacga cgacgacgcg cgacgcaccg agcggctcga ccgcgtcgag gacgtgctgc
     5941 aggagcgcgc gacccgcgag gcggttcagc accttcggcc gcgccgaacg catccgcgtg
     6001 ccccgacccg ctgccatgat caccacgttc aacgccatgc cgccacctgt tccgcaccgc
     6061 gcgcaaaggg cggctgtcga ttatcgcggc gctgcacgat cgcaccgtcg ctgcgcaggc
     6121 ttcggggcag tcagtgcgcc gggatgcgca cccggtgcgg atgggtggcg ttgccgtgcg
     6181 gaaaatcgcg catcgccgcc gcgaacatcg ccggcagcac ctcgagcgga ccgagcgagc
     6241 tgccgctgtt cgtggcgtgt gcctcgtaga gcgcctgtcc gccctgcttg tcgcggatca
     6301 ggatgccgac ttcgcgctcg acatacggaa agtcgtcgat cggcccgggg ccc
//
LOCUS       DASRWS010000095         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_616462, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000095 DASRWS010000000
VERSION     DASRWS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616462"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFF72_01725"
     CDS             <1..1006
                     /locus_tag="VFF72_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HZV91903.1"
                     /translation="TITDPRGTDFAKSDVLGQAPWMLTLERRAIDAFVGLGVAMTDTC
                     INYQTLQAPTRGEHVAFGDTGVVIYSNSICGARSNFEGGPSALAAGLTGRTPRYGFHL
                     DACRRATLRIHVEDTPTSLDQWGALGGVIGRLAGNYWAVPVVEGLEGAPGSDAMKHFG
                     AAMASFGSVALFHLVGLTPEAPRLQDVGGHRLPIAHRIGHADVRALQSSYATSDEVDV
                     VVFSAPQLSLYELRRLAELCEGRRFAKPLLAMTSPQVKPDADRFGYSERIEAAGGHVM
                     SGMCFYQSYAREIAEARGWKRLVTNSAKLVNILGGYGYAPMLASMEQCVDAAETGRLA
                     "
     gene            1003..1458
                     /locus_tag="VFF72_01730"
     CDS             1003..1458
                     /locus_tag="VFF72_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HZV91904.1"
                     /translation="MSAAAVSTAPVRVLVARHAIGATVRGHALVAHDGFSARYDLDRI
                     AGVFWRPAHKLVGQSYVGRILVLDTSKGGVASAWMLREMTSRGVAPLAIVFNSVNPIL
                     VQGAAFGEITMLAGFDGDVTAQVPHGARVEVDPATRRLIVLDSGPAGTG"
     gene            1572..>2132
                     /locus_tag="VFF72_01735"
     CDS             1572..>2132
                     /locus_tag="VFF72_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZV91905.1"
                     /translation="MATPSLQGRKIAILATDGFEQSELLDPRHAFDAAGATTRIVSLK
                     NGQIRAWHKADWGERVAVDSTVAETRADDYDALLLPGGVMNPDKLRTDKAAVAFVKAF
                     LDAGKPVAAICHGPWTIVEAGAAKGRRMTSWPSLQTDLRNAGATWVDEEVVVDGNLVT
                     SRKPDDLPAFCRKIVEEFAEGSHAAQR"
BASE COUNT          313 a          771 c          747 g          301 t
ORIGIN      
        1 gacgatcacc gatccgcgcg gcaccgattt cgcgaagtcc gacgtgcttg gccaggcacc
       61 gtggatgctg acgctcgagc ggcgcgccat cgacgccttc gtcggcctcg gcgtggcgat
      121 gaccgacacc tgcatcaact accagacgtt gcaggcgccg acgcgcggcg agcacgtcgc
      181 cttcggcgac accggcgtcg tcatctactc gaactcgatc tgcggcgccc gctcgaactt
      241 cgaaggcggc ccgtcggcgc tggcggcggg cctcaccggc cgcaccccgc gctatggatt
      301 ccacctcgac gcctgccggc gggcgacgct gcgcatccac gtcgaagaca cgccgacgag
      361 cctcgaccag tggggtgcgc tcggcggcgt gatcggccgt ctcgccggca actactgggc
      421 ggtgcccgtc gtcgaagggc tcgagggagc gcccggctcc gacgcgatga agcatttcgg
      481 cgcggcgatg gcgagcttcg gctcggtggc gctgttccac ctcgtcggcc tgacgcccga
      541 ggcgccgcgc ctgcaggacg tcggcggcca ccgcttgccg atcgcgcacc ggatcggcca
      601 cgccgacgtg cgcgcgctgc agtcgagcta cgccacgtcc gacgaggtcg atgtcgtcgt
      661 cttctcggcg ccgcagctga gcctctacga gctgcgccgg ctcgccgagc tgtgcgaggg
      721 ccgccgcttc gccaagccct tgctggcgat gaccagtccg caggtcaagc ccgacgccga
      781 tcgcttcggc tacagcgagc gcatcgaggc cgcaggcggc catgtcatgt cggggatgtg
      841 cttctaccag tcgtatgcgc gcgaaatcgc cgaggcccgc ggctggaagc ggctggtgac
      901 gaacagcgcc aagctcgtca acatcctcgg cggctacggc tatgcgccga tgctcgcgtc
      961 gatggagcag tgcgtcgacg ccgccgagac ggggcgactc gcgtgagcgc ggccgcggta
     1021 tcgacagcgc cggtgcgcgt cctcgtcgcg cggcacgcga tcggcgcgac ggtgcgcggc
     1081 cacgcgctgg tggcgcacga cggcttctcg gcgcgctacg acctcgaccg catcgccggc
     1141 gtcttctggc ggcccgcgca caagctcgtc ggccagtcgt acgtcggccg catcctcgtg
     1201 ctcgacacct cgaagggcgg tgtcgctagc gcctggatgc tgcgcgagat gacgtcgcgc
     1261 ggcgtcgcgc cgctggccat cgtcttcaac agcgtcaatc ccatcctggt tcagggcgcg
     1321 gcgttcggcg agatcacgat gctcgccggc ttcgacggcg acgtcaccgc gcaggtgccg
     1381 cacggcgcgc gggtcgaggt cgatccggcg acacggcggc tcatcgtgct cgactcgggc
     1441 ccggccggaa cgggctgacg cggccagggg cggcgcccct ttccggcggc gcgcatcgac
     1501 agcgagggca cgcgccttgc catcgaggga cgagggcgcc atggggcgcc gcgccgaaag
     1561 gaaccaccgc catggccacg ccatccctgc agggcagaaa gatcgccatc ctcgccaccg
     1621 acggattcga gcagtccgag ctgctcgatc cacgccatgc gttcgacgcc gccggcgcga
     1681 cgacccgcat cgtctcgctg aagaacggcc agatccgcgc ctggcacaag gccgactggg
     1741 gcgagcgcgt cgccgtcgat tcgaccgtcg ccgagacgcg ggccgacgac tacgacgcgt
     1801 tgctgttgcc gggcggtgtc atgaaccccg acaagctgcg caccgacaag gcagcggtcg
     1861 ccttcgtgaa ggctttcctc gacgccggca agccggtcgc ggcgatctgc cacggcccgt
     1921 ggacgatcgt cgaggccggt gccgccaagg ggcggcggat gacgagctgg ccgtcgctgc
     1981 agaccgacct gcgcaacgcc ggcgcgacct gggtcgacga ggaagtggtc gtcgacggca
     2041 accttgtcac cagccgcaag cccgacgacc tgccggcgtt ctgccgaaag atcgtcgagg
     2101 agttcgccga aggctcgcac gccgcccagc gc
//
LOCUS       DASRWS010000096         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1765346, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000096 DASRWS010000000
VERSION     DASRWS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765346"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="rpsB"
                     /locus_tag="VFF72_01740"
     CDS             <1..435
                     /gene="rpsB"
                     /locus_tag="VFF72_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZV91906.1"
                     /translation="TVKGSLKRLKEMKAQIEAGTEIRIKKEALMFQRELAKLEKDIGG
                     IQDMNALPDAMFVIDVGFHKIAVAEAKKLGIPIIGVVDSNHSPVGIDYVIPGNDDSSK
                     AVALYAKAVADAVLEGKSNAASDVVQSAAAGDEFVEVDEASA"
     gene            559..1479
                     /gene="tsf"
                     /locus_tag="VFF72_01745"
     CDS             559..1479
                     /gene="tsf"
                     /locus_tag="VFF72_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZV91907.1"
                     /translation="MAAITAGMVGALRAKTDAPMMECKKALTEAEGDMDHAEELLRVK
                     LGSKASKASSRIAAEGVVAIAVHGHTGAIIEVNCETDFVTKNDSFLALVQALAERVAK
                     DDPADVAALSALPLAQDGFGPTVEDVRKGLVGKIGENMAIRRFQRYAGAGHLASYLHG
                     TRIGVLVEYEGDDVAAKDVAMHVAAMKPVSLASADVPQSLIELERRIATQKAAEDADK
                     ARAEGKPVQSPEIVARRVEGSVAKFLKEVSLYNQPFVKNDKQTVEQMLKEKATRIKAF
                     TLYVVGEGIEKKAGDFAAEVAAQVAAAKGA"
     gene            1558..2268
                     /gene="pyrH"
                     /locus_tag="VFF72_01750"
     CDS             1558..2268
                     /gene="pyrH"
                     /locus_tag="VFF72_01750"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZV91908.1"
                     /translation="MAAYKRILLKLSGEALMGDDAYGINRATIVRIVREVQEVTRLGC
                     EVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALADTMRQEGITARVMSA
                     IGIEQVVEPYVRPKALQYLEEGKLVIFAAGTGNPFFTTDTAAALRGAEIGAEIVLKAT
                     KVDGVYNADPRKDPAATRYSRISFDEAIIRNLQVLDATAFALCRDQKLPIRVFSIFKP
                     GALKRAVQGEDEGTLVHV"
     gene            2348..2908
                     /gene="frr"
                     /locus_tag="VFF72_01755"
     CDS             2348..2908
                     /gene="frr"
                     /locus_tag="VFF72_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZV91909.1"
                     /translation="MTIADIKKTAEQKMARSIESFKNELQKIRTGRAHPGILDQVHVE
                     YYGSHVPISQVANVTLLDARTISVQPWEKGMGAKIEKAIRESDLGLNPAAQGDLLRVP
                     MPALTEERRRELTKVVRHASEDAKIAIRNLRRDANEHAKRLTKDKEITEDEERRSLDE
                     LQRLTDRTIGEVDRLTAAKEAEIMAV"
     gene            2964..>3638
                     /gene="uppS"
                     /locus_tag="VFF72_01760"
     CDS             2964..>3638
                     /gene="uppS"
                     /locus_tag="VFF72_01760"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZV91910.1"
                     /translation="MSAVVDSAAAAVAVPRHVAIVMDGNGRWARKRYMPRFFGHKQGV
                     DALVRTVLACADRGVEYLTVFAFSSENWKRPSEEVSGLMGLVLVAVSKYLAKLAGDGV
                     RVHIVGDRSQVSDKLREAWQQAEDQTRHNSRIHLSVAFNYGGRWDIVQACRNAIAAGL
                     RPEQVDEERLHSFMALSYAPDPDLFIRTGGEVRISNFLLWQAAYSELVFTDCLWPEFG
                     VAELDAA"
BASE COUNT          668 a         1197 c         1222 g          551 t
ORIGIN      
        1 accgtcaagg gctcgctcaa gcgcctgaag gaaatgaagg cgcagatcga ggccggcacc
       61 gagatccgga tcaagaagga agcgctgatg ttccagcgcg aactcgccaa gctcgagaag
      121 gacatcggcg gcatccagga catgaacgcg ctgcccgacg cgatgttcgt gatcgacgtg
      181 ggtttccaca agatcgccgt cgccgaggcg aagaagctgg gcattcccat catcggcgtc
      241 gtcgactcca atcattcgcc ggtgggcatc gactacgtga ttcccggcaa cgacgactcg
      301 tcgaaggcgg tggcgctcta tgccaaggcc gtcgccgacg ccgtcctcga agggaagtcc
      361 aacgcagcga gcgatgtcgt ccagtcggca gccgccggcg acgaattcgt cgaggtggac
      421 gaagcgtcgg cctgacctcc cgacccggcc cgcccgctcg gcgggcgtgc gacaggggct
      481 gattcagccc cttttttccg atcgatcagg gccggccgcc gctgcgatgg cgccgggcca
      541 cctcagagga gcaagcagat ggcagcgatc accgcaggca tggtcggcgc gctacgcgcg
      601 aagaccgacg cgcccatgat ggaatgcaag aaggccctga ccgaggccga aggcgacatg
      661 gaccacgccg aagagctgtt gcgcgtcaag ctcggcagca aggcctcgaa ggcctcgtcc
      721 cggatcgccg ccgaaggcgt cgtcgccatt gccgtgcacg gccataccgg cgcgatcatc
      781 gaagtcaact gcgaaaccga tttcgtgacc aagaacgact cgtttctcgc cctggtccag
      841 gccctcgccg aacgggtcgc caaggatgac ccggccgacg tggccgcgct gtccgcgctg
      901 cccctcgcgc aggacggctt cggcccgacg gtcgaagacg tcaggaaggg cctcgtcggc
      961 aagatcggcg agaacatggc gatccggcgc ttccagcgtt acgcgggcgc cggccacctc
     1021 gccagctacc tgcacggcac gcggatcggc gttctcgtcg agtacgaagg cgacgacgtc
     1081 gccgcgaagg acgtggcgat gcacgtcgcg gccatgaagc cggtctcgct cgcgtccgcc
     1141 gacgtgccgc agtcgctgat cgagctcgag cggcggatcg cgacgcaaaa ggccgccgag
     1201 gacgccgaca aggctcgcgc cgagggcaag ccggtgcagt cgccggagat cgtcgcccgc
     1261 cgcgtcgaag gatcggtggc gaaattcctg aaggaggtga gcctctacaa ccagcccttc
     1321 gtcaagaacg acaagcagac ggtcgagcag atgctcaagg aaaaggcgac gcgcatcaag
     1381 gcgttcaccc tgtatgtcgt cggcgagggc atcgagaaaa aggccggcga tttcgcggcc
     1441 gaggtggcgg cgcaggtcgc cgcggccaag ggcgcctgag ggcagggcgg ggccgggacg
     1501 ccgggtcctg ggcggataga cttcaccggc gtgcacaacg acgacgacaa gggcgacatg
     1561 gccgcgtaca agcgcatcct cctcaagctt tccggcgaag ctctgatggg ggacgacgcc
     1621 tacggcatca accgggcgac catcgtgcgc atcgtgcgcg aggtccagga ggtgacgcgg
     1681 ctgggctgcg aggtcgccgt cgtgatcggc ggcggcaaca tctttcgcgg tgtcgccggc
     1741 ggctcggtcg gcatggatcg ggccaccgcc gactacatgg gcatgctggc caccgtgatg
     1801 aacgcgctcg ccctggccga cacgatgcgc caagagggca tcacggcgcg ggtcatgtcg
     1861 gcgatcggca tcgagcaggt tgtcgagccg tacgtccggc ccaaggcctt gcagtatctc
     1921 gaggaaggca agctcgtcat cttcgcggcc ggcaccggca atccgttttt caccaccgac
     1981 acggcagcgg cgctgcgcgg ggcggagatc ggagccgaga tcgtcctcaa ggcgaccaag
     2041 gtcgacggtg tctacaacgc cgatccgagg aaggatccgg cggcgacgcg ttattcgcgg
     2101 atcagcttcg acgaagccat catccgcaac cttcaggtgc tcgatgcgac ggcgttcgct
     2161 ctttgtcgcg accagaagct gccgatccgc gtcttttcga tcttcaagcc cggagccctg
     2221 aagcgcgccg tccagggcga ggacgaaggc acgctcgttc acgtgtgagg aacatgatgg
     2281 aacctttccg cccctgtccc acgcccggcc ttcgaggccg cgtgccggct tcctgcgcgg
     2341 ggcggcgatg acgatcgccg acatcaagaa gacggccgag cagaagatgg cccgctcgat
     2401 cgagtcgttc aagaacgagc tgcagaagat ccgcaccggc cgtgcccacc cgggaatcct
     2461 cgatcaggtg cacgtcgagt actacggctc gcacgtgccg atcagccagg tcgcgaacgt
     2521 gaccttgctc gatgcccgca ccatcagcgt ccagccctgg gaaaagggca tgggcgcgaa
     2581 gatcgagaag gcgattcgcg aatcggatct gggcctcaac ccggcggcgc agggcgactt
     2641 gctgcgcgtt cccatgccgg cgctcaccga ggagcgccgg cgcgagctca cgaaggtcgt
     2701 tcgtcacgcc agcgaggacg ccaagatcgc gattcgcaac ttgcgtcgcg acgccaacga
     2761 gcatgccaag cggctgacca aggacaagga gatcaccgag gacgaggagc gtcgctccct
     2821 cgacgagctg cagcgcctca ccgatcgcac gatcggcgaa gtcgatcgcc tgaccgccgc
     2881 caaggaagcc gagatcatgg cggtctgatg ggcgccttcg gccgttcgcg cctgccgcga
     2941 cgtcatctct gcctgcgact tcgatgagcg ccgtggtcga ttccgctgcc gccgcggttg
     3001 ccgtgccgcg ccatgtcgcg atcgtcatgg acggcaacgg ccggtgggcg cgcaagcgct
     3061 acatgccgcg cttcttcggc cacaagcagg gcgtcgacgc gctggttcgc acggtcctcg
     3121 cctgcgccga ccgcggcgtg gagtacctga cggtgttcgc tttctcttcc gagaactgga
     3181 agcggccgag cgaggaggtc tcgggactga tgggcctggt gctcgtcgcg gtgtcgaaat
     3241 atctcgccaa gctcgccggc gacggagttc gggtgcacat cgtcggcgat cgcagtcagg
     3301 tctccgacaa gttgcgcgag gcctggcagc aggccgagga ccagacccgc cacaactcgc
     3361 gcatccatct gtcggtcgcc ttcaactatg gtggccgctg ggacatcgtg caggcctgcc
     3421 gcaacgccat tgccgccgga ctgcgtcccg agcaggtcga cgaggaacga ctgcacagct
     3481 tcatggcgct cagctatgcg cccgatcccg acctcttcat ccgcaccggt ggcgaggtcc
     3541 gcatcagcaa tttcctgctg tggcaggcgg cctactcgga gctcgtcttc acggattgcc
     3601 tgtggccgga attcggcgtc gccgagctcg acgccgca
//
LOCUS       DASRWS010000097         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1188429, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000097 DASRWS010000000
VERSION     DASRWS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188429"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFF72_01765"
     CDS             complement(<1..380)
                     /locus_tag="VFF72_01765"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZV91911.1"
                     /translation="MALDLVPLWTAILALAVFMYVLLDGFDLGVGMLFALRRDAADRD
                     LMITSVAPVWDFNETWLVLGGGGLLAVFPLAFAVLIPALYFPILAMLLGLLFRGVAFE
                     FREVEGARRSVWDAGFVGGSFVAT"
     gene            complement(370..1788)
                     /locus_tag="VFF72_01770"
     CDS             complement(370..1788)
                     /locus_tag="VFF72_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZV91912.1"
                     /translation="MDALLLSRLQFAWVIALHILLPAFTVGLACYIATLETLWWLKKD
                     DVYRRLSAFWIKIFAVSFGMGVVSGIVMPFQFGTNWSRYVDATANVVGGLMAYEVLTA
                     FFLESAFLGVLLFGRKLVPQWAHVFSALMVALGTLLSSFWILAVNSWMQTPTGYKIVD
                     GRFFPVDMFAVIFSPSFPYRLTHTVIAFVVTTAFVVLGVGATYLRQQRHASESKVMVR
                     MSLFFLTVMVPLQIAVGDLHGVNTLEHQPAKLAAMEGLWDTGRGVAASLFAIPDAQAE
                     TNRFEIAIPNLASLYLTHDWHGEVKGLKAWPRQDRPPVAIVYFAFRVMVGVGMLMLGV
                     VATGWWQKSRGRLFTSPFFLRAAQWATPIGFVAVIAGWTVTEVGRQPWVVYGLMRTRD
                     AVSPSLTTGDVALSLASYVLAYIVIFGGGFVLLRRMVRRGPPAAAAEEGREDTQSRAA
                     RPLSAATQAASSGGATGASDGA"
BASE COUNT          427 a          680 c          686 g          247 t
ORIGIN      
        1 aaggtggcga cgaaggagcc cccgacaaag cctgcgtccc agacgctgcg ccgcgccccc
       61 tcgacctcgc gaaactcgaa cgcgacgcct cgaaacagga ggccgagcag catggccagg
      121 atcgggaaat aaagcgccgg aatcaggacc gcgaaggcca gcggaaagac tgcgagcagg
      181 ccaccgccgc cgaggacgag ccaggtctcg ttgaagtccc acaccggcgc caccgacgtg
      241 atcatgaggt cccggtcggc ggcatcgcga cgaagggcga agagcattcc gacgccgagg
      301 tcgaagccgt cgagcagcac gtacatgaac acagcgagcg cgaggatcgc cgtccacagc
      361 ggcacgaggt caagcgccat cggaagctcc cgtcgcgccg ccgctgctcg cggcctgggt
      421 ggccgcagaa agcggccttg ccgcgcgcga ttgcgtgtct tcgcgccctt cctctgccgc
      481 cgccgcgggc ggcccgcgcc gcaccatgcg ccgcagcagg acgaagccgc cgccgaagat
      541 gacgatgtag gcgagcacgt aggaggccag cgagagcgcc acatcgccgg tcgtgagcga
      601 aggcgatacc gcgtcgcgcg ttcgcatcag tccgtacacg acccacggct gtcgcccgac
      661 ctcggtcacg gtccagccgg cgatcacggc aacgaagccg atcggtgtcg cccactgcgc
      721 tgcgcgaagg aagaacggcg aggtgaagag gcggccgcgc gacttctgcc accagcccgt
      781 cgcgacgacc ccaagcatca gcatgccgac gcccaccatg acgcgaaagg cgaaatagac
      841 gatggcgacg ggcgggcggt cttggcgcgg ccacgccttg agccccttca cctcgccgtg
      901 ccagtcatgc gtgaggtaca ggctcgccag attgggaatc gcgatctcga agcggttggt
      961 ctccgcctgc gcgtcgggga tcgcgaagag cgaggcggcg acgccgcgcc ccgtgtccca
     1021 gagcccctcc atcgccgcca gcttggcggg ctggtgctcc agcgtgttga cgccgtgcag
     1081 atcgcccacc gcgatctgca gcggcaccat gacggtcagg aagaagagcg acatccgcac
     1141 catcaccttg ctctcgcttg catggcgctg ctggcgcagg tacgtggcac cgacgccgag
     1201 aacgacgaat gccgtcgtga cgacgaaggc gatgacggtg tgcgtcagcc ggtacgggaa
     1261 cgacggcgaa aagatcacgg cgaacatgtc gaccggaaag aacctgccgt cgacgatctt
     1321 gtatccggta ggcgtctgca tccagctgtt gacggcgagg atccagaacg acgagagcag
     1381 cgtgccgagc gccaccatca gcgcggagaa cacgtgcgcc cattgcggca cgagcttgcg
     1441 accgaagaga agcaccccga ggaaggcgga ttcgagaaag aaggcggtga ggacttcgta
     1501 ggccatcagg ccgccgacga cgttcgcggt cgcatcgacg tagcggctcc agttggtgcc
     1561 gaactggaac ggcatgacga tgcccgacac gacgcccatg ccgaatgaga ccgcgaagat
     1621 cttgatccag aaggccgaaa ggcgccgata gacgtcgtcc ttcttcagcc accagagcgt
     1681 ctccagcgtc gcgatatagc aggcgaggcc gaccgtgaac gccggcagga ggatgtgcag
     1741 cgcgatcacc caggcgaact gaagccgcga cagcagcaag gcgtccaatg agagctccca
     1801 tcaccgaaga actcgcgaat gacggatcgt cacgcgccac cgggacgcat cggcgacccg
     1861 cgagcgcgaa agctaccccg gcgccgggga atgcgtcaaa tccatgctgg cgagatgtcc
     1921 acgcagcgaa acacgctgtg gcgacgccgc aggctcgccc gggccgccgc cgcgagcgtc
     1981 ggcacacggc cggcgaggtc ggtgaatccc ctgtaagaga acggcgacct gcgggaggac
//
LOCUS       DASRWS010000098         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3780890, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000098 DASRWS010000000
VERSION     DASRWS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3780890"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2220
                     /gene="dxs"
                     /locus_tag="VFF72_01775"
                     /pseudo
     CDS             299..2220
                     /gene="dxs"
                     /locus_tag="VFF72_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
BASE COUNT          371 a          794 c          770 g          364 t
ORIGIN      
        1 cgctgcgggc cagcggcctc gatcgcgtgc agggcctgct gacattggcc gatcgggtcg
       61 tcgatcgcga aagctgaatg aggcccgcac gctccgttcg gtcgctgcct cccgaggggg
      121 cgaccgctgg tggaccggca aggccggatc catggcggtg gcttggatgg ccgcttcatc
      181 gccgccggac cactactgag atcaacacgt cttgctcacc gtggctcgct gacccgcgtg
      241 gcgacgcacg cggtcggacc agcgaagccg tgtccattga tgtggaagcg gcgcgtcgat
      301 gagcagcaag ctgctcgact cgattgccac gcccgccgac gtgcgccggc tgtcgcggcc
      361 cgagctgaag cagctcgccg gcgagctgcg cgatttcgtc ctgcaaagtg tctcgcagac
      421 cggtggccat ctgtcgtcga atctcggcac cgtcgagctg acgatcgcgc tgcaccatgt
      481 cttcgacacg ccgttcgacc gtatcgtctg ggacgtcggc caccagacct atccgcacaa
      541 ggtgctcacc ggccggcgcg agcggatggc gacgctgcgc cagctcggcg gcatctccgg
      601 ctttccgcgt cgcgacgaga gcgagtacga caccttcggc accgcgcatt cgtcgacctc
      661 gatctcggcc gccctcggca tgccgccgcg tcgcgcctga agaatgagca gcggcgctgc
      721 gtggcggtga tcggcgacgg cgcgatgagc gccggcatgg cgttcgaggc gatgaacaac
      781 gcgggctatg cgcgagaggg cagcgagccg cccgacgtgc tcgtcatcct caacgacaac
      841 gacatgtcga tctcgccgcc ggtgggtgcg ctcaaccggt acctggcacg gctgatgagc
      901 ggcaagttct acgccgcggc gcgcgacacc gccaagacgg tgctgaagaa cgcgccgccg
      961 ctcttcgagc tggcgcgccg cttcgaggag cacgccaagg gcatggtcgt gccgggcacg
     1021 atcttcgagg agttcggctt caactacgtc ggcccgatcg acggccacga tctcgattcg
     1081 ctgattccca cgctcgagaa cctgcgccag ttgaagggtc cgcagttcct gcacgtcgtc
     1141 accaagaagg gctacggcta caagctggcc gagaacgatc cggtcaacta ccacgggccg
     1201 agcaagttcg atccgaaggt gggcatcgtc acgccggcga cgccgccgaa gatgaccttc
     1261 acgcaagtgt tcgggcaatg gctgtgcgac atggccgccc gcgacgatcg gcttgtcggc
     1321 atcacgccgg cgatgcgcga gggctcgggc atggtcgagt tcgagaaacg cttcccgaaa
     1381 cgctacttcg acgtcggcat cgccgaacag cacgccgtga cgttcgcggc cggcctggcg
     1441 tgcgaaggca tgaagccggt ggtcgcgatc tactcgacct tcctgcagcg cgcctacgac
     1501 cagctcatcc acgatgtcgc gatccagaac ctgccggtgg tcttcgcact cgaccgcgcc
     1561 ggcctcgtcg gcgccgatgg cgcgacgcat gccggtgcct acgacatcgc ctacctgcgc
     1621 tgcgtgccca acgtcagcgt gctgacgccg gccgacgagg acgaatgccg gcagtcgctc
     1681 tacaccgcgt tcctgcagag ccatccggtc gccgtgcgct atccgcgcgg cagcggtgtc
     1741 ggccgcctgc cgcaggcggc catgacggag atcccgtggg gcaagggcga gatccggcgc
     1801 gagacctcga aggtcggacc ggcggccggg cagcggatcg ccatcctcgc cttcggaacg
     1861 ctcctctacc cggcgctcgc ggccgccgag aagctcgacg cgaccgtcgc caacatgcgg
     1921 ttcgccaagc ctctcgatgt cgcgctggtg actgaactgg cgcgccgcca tgatgcaatc
     1981 gtcaccgtgg aagaaggaac cgttctgggt ggtgccggca gcgccgtgct ggaggcgctg
     2041 gccgaggcgc ggatcgaggt tccggtgctg acgctgggcc tggccgacag cttcagcgag
     2101 cacggcgatc cggcgaagct cctcgccctc tacgggctcg acgccgccgg gatcgagcag
     2161 cagatccggc gtcgcttcgg cgccgcgccg gcgctggttc gcagcgctgc aggtctttga
     2221 ggcccggggg acaatccccg ccatcatgtc caacctcatc gatgcgcttc atattcccga
     2281 cacccagtcg gcgcacgac
//
LOCUS       DASRWS010000099         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3781037, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000099 DASRWS010000000
VERSION     DASRWS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781037"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFF72_01780"
     CDS             complement(<1..473)
                     /locus_tag="VFF72_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZV91913.1"
                     /translation="MTTRGKGKKLNKAWLHDHLTDPYVKLAQREGYRARAAYKLKEID
                     ETLQLLRPGQLVVDLGAAPGAWSQYVRRRFGRDGEPSGPMHGTLIALDMLDFQPVEGV
                     DFIQGDFRELPVLEALEARLDGRRVDVVLSDMAPNLSGIASADAARMSELVELAV"
     gene            512..985
                     /locus_tag="VFF72_01785"
     CDS             512..985
                     /locus_tag="VFF72_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HZV91914.1"
                     /translation="MNALVLTPAQRKQKRAEAHHLDPVVSIGNEGATAAVRREIDAAL
                     AAHGLVKVRVFSDDRSVREALLVEIGDSLGAAPVQHIGKLLVFWRPPAPREKADREGR
                     MPGPKIVKVLKFSKSGNHRPQVKKVRVVGNMRLAAGGQLKRARKRVTSVRKSQVD"
     gene            complement(963..1454)
                     /locus_tag="VFF72_01790"
     CDS             complement(963..1454)
                     /locus_tag="VFF72_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HZV91915.1"
                     /translation="MASDAAAVKTTTLAAFGAAIAAGWAGAMTGIGLVAAPVVFDVLP
                     RVDAGRVAARLFGAEATIGLCVGAVLMVIGVQLGRERAELGRGSRFGRELVLALCALA
                     CVIVGYYALQPLLEPARAGHGPLSFAALHGFASLLFVARIVLAALLAWQLGRSSINRP
                     AIS"
     gene            complement(1459..1935)
                     /gene="greA"
                     /locus_tag="VFF72_01795"
     CDS             complement(1459..1935)
                     /gene="greA"
                     /locus_tag="VFF72_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZV91916.1"
                     /translation="MATIPLTRRGADKLKEELARLKSVDRHAVIQAIAEARAQGDLSE
                     NAEYEAAKDKQGFIEGRILEIEGKLAAAQIIDPSTIDAEGRVVFGSTVDLEDEDSGAK
                     VTYQIVGDDEADLKEGRISISSPIARALIGKESGDIAEVQAPGGTRSYEVVAVRYA"
     gene            complement(2020..>3135)
                     /gene="carB"
                     /locus_tag="VFF72_01800"
     CDS             complement(2020..>3135)
                     /gene="carB"
                     /locus_tag="VFF72_01800"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641645.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZV91917.1"
                     /translation="VVRPSYVLGGRAMEIVHGDKDLERYMREAVRVSEKSPVLLDRFL
                     DDAIEVDVDCIADGSEVMIGGIMEHVEQAGIHSGDSACSLPPYSLPASLQDEMRRQTR
                     SMAEALGVVGLMNVQFAIQGDTSGDAPNAVVYVLEVNPRASRTVPFVSKATAQPLAKI
                     AARCMVGQKLSTQKGVRGEVVPGYFSVKEAVFPFNKFPGVDPILGPEMRSTGEVMGTG
                     RSFGEAMLKSQLGAGSQLPSQGNVCITVRNGDKARAVVVARDLHRLGFGLVATRGTAA
                     AIAAAGIPVRAVNKVKDGRPHIVDLLKGGEIQLVFTTVDETRTAIADSRHIRQAALAH
                     RVTYYTSMAGCEAAVEGMKHRDDLEVMALQDLHRALA"
BASE COUNT          513 a         1105 c         1031 g          486 t
ORIGIN      
        1 tcgactgcca gctcgacgag ctcgctcatg cgggcagcgt cggcggaggc gattcccgag
       61 aggttgggcg ccatgtctga caggacgacg tcgacgcggc gaccgtcgag gcgcgcctcc
      121 agcgcctcga gcaccggcag ctcgcgaaag tcgccctgga tgaaatcgac cccctcgacc
      181 ggctggaaat cgagcatgtc cagcgcgatc agcgttccgt gcatgggccc ggaaggctcg
      241 ccgtcgcgcc cgaacctgcg ccgcacgtac tggctccagg cgcccggcgc cgcccccagg
      301 tcgacgacaa gctgcccggg gcgcaacaac tgcaaggtct cgtcgatctc cttcagcttg
      361 taggcggcgc gcgcccgata gccctcgcgc tgcgcaagct tgacgtaggg atcggttaga
      421 tggtcgtgca gccacgcctt gttcagcttc tttcccttgc cccgcgtcgt catcgtgccg
      481 ctttcgtcat ggccggctcg ataataggca ggtgaacgca ctcgtcctga cccccgccca
      541 acgcaagcag aagcgcgccg aggcgcacca tctcgacccg gtcgtgtcga tcggcaatga
      601 aggagcgacg gccgcggttc gccgcgagat cgacgcggcg ctcgccgcgc acgggctggt
      661 caaggtccgc gtcttctccg acgatcggtc cgtccgtgaa gccctgctgg tcgaaatcgg
      721 cgattcgctc ggggcggctc cggtacagca catcggcaag ctgctggtct tctggcggcc
      781 gcccgcaccg cgcgagaagg ccgaccgcga aggccggatg cccgggccga agatcgtcaa
      841 ggtgctcaaa ttctcgaaga gcggcaatca tcgcccgcaa gtgaagaagg tcagggtcgt
      901 cggcaacatg cggctggccg ccggcggcca gctcaagcgc gccaggaagc gcgtcaccag
      961 cgtcaggaaa tcgcaggtcg attgatcgac gaccggccga gttgccaagc gagcaaggca
     1021 gccagcacga tccgggcgac gaacagcagg gaagcgaagc cgtgcagcgc cgcgaacgac
     1081 aggggcccgt gcccggcccg cgccggctcg agcaagggct gcaacgcgta gtagccgacg
     1141 atcacgcagg ccagcgcaca cagcgccagg acgagctccc ggccaaaccg tgatccgcgt
     1201 ccgagttcgg cccgctcccg accgagctgc acgccgatga ccatgagcac cgcgcctacg
     1261 cacaggccga tcgtcgcctc ggcgccgaac agccgcgccg cgacgcgacc ggcgtcgacg
     1321 cgcggcagga cgtcgaagac caccggcgcg gcgaccagtc cgatcccggt catggcaccg
     1381 gcccagccag ccgcgatcgc ggcgccgaat gcggccagcg tcgtcgtctt caccgcagca
     1441 gcgtcgctcg ccacgcgctc aggcgtagcg aacggcgacc acctcgtagc tgcgggtgcc
     1501 tcccggcgcc tggacttcgg cgatatcgcc actctccttg ccgatgagcg cacgggcgat
     1561 cggcgagctg atcgagatgc ggccttcctt caggtcggcc tcgtcgtcgc cgacgatctg
     1621 gtacgtcacc ttggcgccgg aatcctcgtc ctcgagatcg accgtcgatc cgaacacgac
     1681 gcggccttcg gcatcgatgg tcgacgggtc gatgatctgc gccgccgcga gcttgccctc
     1741 gatctcgaga attcgcccct cgatgaatcc ctgcttgtcc ttggccgcct cgtactcggc
     1801 gttctcggaa agatcgcctt gcgcgcgcgc ctcggcgatg gcctggatga ccgcgtgacg
     1861 atcgacgctc ttcagacgcg cgagctcctc cttcagtttg tcggcaccac ggcgggtgag
     1921 cggaatcgtc gccatcgaag cttcctcgca agccaaaatg aaaccgccgc cgggaactcc
     1981 gcggcggcgg cacttgttcg gagtcagttt agtgcagaac taggccagcg ccctgtgcag
     2041 atcctgcaag gccatgacct cgaggtcgtc gcggtgcttc atgccttcga cggcggcttc
     2101 gcagccggcc atgctggtgt agtaggtgac gcgatgggcg agcgccgcct ggcggatgtg
     2161 gcgggaatcg gcgatcgcgg tccgtgtctc gtcgaccgtc gtgaagacga gctggatctc
     2221 gccgcccttg agcaggtcga cgatgtgcgg acggccgtcc ttgaccttgt tgaccgcgcg
     2281 caccggaatg cccgccgcgg cgatcgccgc cgccgttcca cgcgtcgcga cgagcccgaa
     2341 gccgagccgg tggaggtcgc gggcgacgac cacggcgcgg gccttgtcgc cattcctgac
     2401 ggtgatgcag acgttaccct ggctcggcaa ctgcgatccg gcgccgagct ggctcttcag
     2461 catcgcctcg ccgaagctgc gaccggtgcc catgacctcg ccggtcgagc gcatctcggg
     2521 tccgaggatc ggatcgacgc ccgggaactt gttgaacgga aagacggctt ccttgaccga
     2581 gaagtatccg ggcacgacct cgccgcggac tcccttctgg gtcgacagct tctggccgac
     2641 catgcaccgg gcggcgatct tggcgagcgg ctgcgccgtc gccttggaca cgaacggcac
     2701 cgtccgcgac gcccgtggat tgacctcgag cacgtacacg acggcgttcg gcgcgtcgcc
     2761 gctggtgtcg ccctggatcg cgaactgcac gttcatcagg ccgacgacgc ccagcgcctc
     2821 ggccatcgac cgggtctgcc gccgcatctc gtcctgcagc gacgccggca gcgaataggg
     2881 cggcagcgag caggccgagt cgccggaatg gatgcccgcc tgctcgacgt gctccatgat
     2941 gccgccgatc atcacctcgc tgccgtcggc gatgcagtcg acgtcgacct cgatcgcgtc
     3001 gtcgaggaag cgatcgagca gaaccggcga tttctcgctc acgcgcacgg cctcgcgcat
     3061 gtagcgctcg agatccttgt cgccgtgcac gatctccatc gcgcggccac cgagcacgta
     3121 gctcgggcgc acgac
//
LOCUS       DASRWS010000100         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3781151, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000100 DASRWS010000000
VERSION     DASRWS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781151"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFF72_01805"
     CDS             complement(<1..439)
                     /locus_tag="VFF72_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91918.1"
                     /translation="MRTALIACLFLACGAAGAAAQDGNCRNFSWSVGRTIDLFDEPLP
                     TVQSAQSLPKEGVFALQLRPVADVIYMMTPERGSDGSWGAMITLENVPAGRYQIALSD
                     DAWVEAVQDNKRLVTFASKRSKECRGVRHSVDVDVQSNPLTLQI"
     gene            complement(453..1436)
                     /gene="glpX"
                     /locus_tag="VFF72_01810"
     CDS             complement(453..1436)
                     /gene="glpX"
                     /locus_tag="VFF72_01810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZV91919.1"
                     /translation="MAEVGKMDRNLALEAVRVTEAAALAASKLMGRGDEKAADQAAVD
                     AMRSALNTLSIDGTVVIGEGERDEAPMLYIGEKVGSGGPKIDIALDPLEGTTITAKGL
                     PNGLAVLAMAEDGNFLNAPDVYMDKIAVGGGLPDGVVDLDKSAKQNLADLAQAKKVQV
                     SDLVVCILDRPRHSELIAKVREAGARIMLIGDGDVSGVIATSTGDSGIYIYMGSGGAP
                     EGVLAAAALRAIGGQMQGRLLFRNDDEKARAKKWGITDLNRKYSMLDMAKGDVMFAAT
                     GVTSGSMLKGVRRFGNGAETHSIVMRSKTGTVRWISAHHNFSIKTGLPSWA"
     gene            complement(1473..2765)
                     /locus_tag="VFF72_01815"
     CDS             complement(1473..2765)
                     /locus_tag="VFF72_01815"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZV91920.1"
                     /translation="MTGPLKIGIAGLGTVGAGVVKLLAEHNRLLSLRGGRRLKLVAVS
                     ARSKAKKRDIDLDGVRWEKDPLELATAPDIDVVVELIGGSSGVAKRLAERALRSGKHV
                     VTANKALLAMHGAELAALAEKRGAILAFEAAVAGGIPIIKALREGLVGNRVGRLYGIL
                     NGTCNYILTTMRETGRDFDVVLAEAQAAGYAEADPSFDVDGIDAAHKLAVLTGAAFGA
                     RVNFAGIHVEGIRRVTSMDIGFAEELNHRIKLLGLARETPYGIEQRVHPCLVPLSAPI
                     AHIEGVFNAVAIEGDFVGKTMFQGRGAGKGPTASAVVADIIDVARGRHMPAFVVPADK
                     LADKKAAPMARHVGAYYIRLMVQDRPGVIAAISKALAEERISLESMIQRGRSDAGEVP
                     VVLTTHETEEARMQRALSRIAKLKAVAEEPCLIRIESF"
BASE COUNT          438 a          997 c          943 g          487 t
ORIGIN      
        1 cgatctgcag ggtaagtggg ttgctctgca cgtcgacatc gacgctgtgg cggacgcccc
       61 ggcattcctt ggagcgcttc gaggcgaagg tgacgaggcg cttgttgtcc tggaccgcct
      121 cgacccaggc gtcgtccgac agggcgatct ggtagcggcc ggccggcacg ttctccagcg
      181 tgatcatcgc gccccacgag ccgtcgctgc cgcgctcggg cgtcatcatg tagatcacgt
      241 cggcgaccgg cctcagctgc agcgcgaaca cgccttcctt gggcagcgac tgcgcgctct
      301 gcacggtcgg cagcggctcg tcgaacaggt cgatcgtgcg gccgaccgac caggagaaat
      361 tgcggcaatt cccgtcctgc gccgcggctc cggccgcgcc gcaggcaaga aaaagacagg
      421 cgatcagcgc tgtccgcatg cctccatcat gatcaggccc agctcggcag gccagtcttt
      481 atcgagaaat tgtgatgggc agaaatccac cggaccgtgc ccgtcttgga gcgcatgacg
      541 atggaatggg tctccgcgcc attgccgaag cggcggacgc ccttgagcat cgagcccgag
      601 gtgacgccgg tggcggcgaa catcacgtcg cccttggcca tgtcgagcat cgagtatttg
      661 cgattgaggt cggtgatgcc ccacttcttg gcccgcgcct tctcgtcgtc gttgcggaac
      721 agcagccggc cctgcatctg gccgccgatc gcgcgcagcg ccgccgccgc cagcacgcct
      781 tccggcgcgc cgcccgagcc catgtagatg tagatgcccg aatcgccggt cgaggtggcg
      841 atcacgcccg acacgtcgcc gtcgccgatc agcatgatgc gggcaccggc ctcgcgcacc
      901 ttggcgatca gctcggagtg gcgcggccgg tcgaggatgc agacgacgag atccgacacc
      961 tggaccttct tggcctgggc gaggtcggcg aggttctgct tggccgactt gtcgagatcg
     1021 accacgccgt cgggcaggcc gccgccgacc gcgatcttgt ccatgtagac gtcgggcgca
     1081 ttgaggaagt tgccgtcctc ggccatcgcc agcacggcga ggccgttggg caggcccttg
     1141 gcggtgatgg tcgtaccctc cagcggatcg agcgcgatgt cgatcttcgg gccgcccgag
     1201 ccgaccttct cgccgatata gagcattggc gcctcgtcgc gctcgccctc gccgatcacg
     1261 acagtcccgt cgatcgaaag ggtgttgagc gccgagcgca tcgcatcgac ggcggcctgg
     1321 tcggccgcct tctcgtcgcc gcggcccatc agcttggatg cagccagcgc agcggcctcg
     1381 gtcacacgca cggcctcgag ggccaggttg cggtccatct tgccaacttc ggccattgct
     1441 tcctccatcg gcgccttccc agcgcctttt cctcagaacg actcgatgcg gatcaggcag
     1501 ggctcctcgg ccactgcctt cagcttcgca atgcgcgaca gcgcgcgctg catgcgcgcc
     1561 tcctcggtct catgcgtggt cagcaccacc ggcacctcgc cggcgtcgga ccgcccgcgt
     1621 tgtatcatcg actcgagcga aatgcgctcc tcggccagcg ccttggagat cgcggcgatc
     1681 acgccgggac ggtcctgtac catcagacgg atatagtagg caccgacatg gcgggccatt
     1741 ggcgccgcct tcttgtcggc gagcttgtcg gccggcacga caaacgccgg catgtgccgc
     1801 ccgcgcgcca cgtcgatgat gtcggccacc accgccgaag cggtcggccc cttccccgcg
     1861 ccgcggcctt ggaacatggt cttgccgacg aagtcgccct cgatcgccac ggcattgaac
     1921 acgccctcga tatgcgcgat cggcgccgac agcggcacta ggcagggatg cacgcgctgt
     1981 tcgattccgt acggtgtctc gcgcgcgagg cccagcagct tgatgcgatg gttcagctcc
     2041 tcggcgaagc cgatgtccat cgaggtgacg cggcggatgc cctcgacgtg gatgccggcg
     2101 aaattgaccc gggcaccgaa cgccgcaccg gtcagcaccg cgagcttgtg cgcagcgtcg
     2161 attccgtcga cgtcgaacga cggatcggcc tcggcgtagc ccgccgcctg tgcctcggcc
     2221 agcacgacgt cgaagtcgcg gccggtctcg cgcatggtgg tcaggatgta gttgcaggtg
     2281 ccgttgagga tgccgtagag gcgccccacc cggttgccga ccaggccctc gcgcagcgcc
     2341 ttgatgatcg ggatgccgcc cgcgactgcc gcctcgaagg cgaggatcgc gccgcgcttc
     2401 tcggccagcg ccgcgagctc agcgccgtgc atggcgagca gcgccttgtt ggccgtgacg
     2461 acatgcttgc ccgaccgaag tgcccgctcg gccaggcgct tcgcaacgcc gctcgagccg
     2521 ccaatcaact cgacgacgac atcgatgtcg ggcgccgtgg cgagctccag cgggtccttc
     2581 tcccagcgca cgccatccag gtcgatgtcg cgcttcttgg ccttggagcg tgcgctcacg
     2641 gccaccagct tgaggcgccg gccgccgcgc agcgacagca ggcgattgtg ctcggcgagc
     2701 agcttgacga cgcccgcgcc tacggtgccg aggccggcga tgccgatctt caggggtcca
     2761 gtcatggtcg tcgtctagtt caggcgcgcg ccttgagcgg cgtgatgttg tcaccgacgc
     2821 cgcgcaggta gagatcgatc gatcgcgagg gatcgatcat cacct
//
LOCUS       DASRWS010000101         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1766063, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000101 DASRWS010000000
VERSION     DASRWS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766063"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VFF72_01820"
     CDS             complement(<1..1143)
                     /locus_tag="VFF72_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV91921.1"
                     /translation="MQTTFPKLLLQHAAERPGAAALREKEFGIWQTLSWAELAQLVRA
                     IACGLADAGVVRGAHLVVIGENRPRLSAAMLAAQVLGAIPVPLYQDAVAAEFVFPIRN
                     ADVAFAIVEDQEQVDKLLEVRGDCPLLERIWFDDPRGLRHYEVDGLAPLDRLIEAGRA
                     RDAADPGFFDGAVARTQPGDVAAMFFTSGTTGSAKGVVHTHATLIDRAAAGARFEKLG
                     PDDEVLAYLPPAWIGQNIFSYAQWLVCGYVVNCPESASTVTIDLKEIGPTYYFAPPRV
                     FEALLTSVMIRMEDASRPKRWMFERAMALARRVGPALMDGQPVGPLDRLRYALGNLLV
                     YAPLRNTLGFSRVRIAYTAGEAIGPDLFSFYRSIGINLKQLYGQTET"
     gene            complement(1308..>2774)
                     /locus_tag="VFF72_01825"
     CDS             complement(1308..>2774)
                     /locus_tag="VFF72_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZV91922.1"
                     /translation="IGLFCPNRIVDPPRMDDARLFRHPPGYRTVALKPVFDWSAWNVQ
                     NDPLTEATTPPGSGGDPSGGLAKAVEMCNNNGHCRKFDAGTMCPSYRVTRDEQHVTRG
                     RANTLRLALSGQLAGVDEAAPLAADAVAEALDLCVGCKGCKRDCPTGVDMARMKFEVA
                     AQRKAERGHSLRDRLIGTLPDLAPRLSRIASLANLPGRSRWLRRLVERSVGLSAKRPL
                     PRWRRDTFARVAASLGLADRDATLAATKAVVLFVDTFNGHFETENALAAVRVLRAAGY
                     TVHVARADDGRPLCCGRTYLAAGMPERARAKAKALLAALLPFAERGIGIVGLEPSCLF
                     TLRDEMLVMGLGAPATRVAAQAMLFEEFITRESHAGRMALHLKTADRPIVVHGHCHQK
                     AFGAMPAVLEALRLIPGARPSLIESSCCGMAGSFGYEAEHHAVSMQMAELSLLPAVRG
                     APGDAWIVADGTSCRQQIADGAGREALHVARVLAGHLA"
BASE COUNT          421 a          941 c         1018 g          394 t
ORIGIN      
        1 ggtctcggtc tggccgtaga gctgcttgag gttgatgccg atcgatcggt agaaggaaaa
       61 gagatcgggg ccgatcgcct cgccggcggt gtaggcgatg cgcacccgcg aaaagccgag
      121 cgtgttgcgc agcggggcgt agacgagcaa gttgccgagc gcgtagcgca gccggtcgag
      181 cggcccgacc ggctggccat ccatcagcgc cggaccgacg cgacgggcca gcgccatcgc
      241 ccgctcgaac atccagcgct tgggccggct cgcgtcttcc atgcggatca tgacgctggt
      301 gagcagcgcc tcgaagaccc gcggcggcgc gaagtagtag gtcgggccga tctccttcag
      361 gtcgatcgtc accgtgctcg ccgactcggg gcagttgacg acgtagccgc agaccagcca
      421 ttgcgcgtag gagaagatgt tctggccgat ccatgccggc ggcagatagg cgagcacctc
      481 gtcgtcgggg ccgagcttct cgaaccgcgc accggcggcg gcgcgatcga tcagcgtggc
      541 gtgggtgtgc acgacgccct tggcgctgcc ggtcgtgccc gacgtgaaga acatcgccgc
      601 gacgtcgccg ggctgcgtcc gggcgaccgc gccgtcgaag aaaccggggt cggcggcgtc
      661 gcgcgcgcgg ccggcctcga tcagccgatc gagcggcgcc aggccgtcga cctcgtaatg
      721 gcgcaggccg cgcggatcgt cgaaccagat ccgttcgagc aggggacagt cgccgcgcac
      781 ctcgagcagc ttgtcgacct gctcctggtc ctcgacgatg gcgaaggcga cgtcggcgtt
      841 gcggatcggg aagacgaact cggcggcaac cgcgtcctgg tagaggggca ccggaatcgc
      901 cccgaggact tgcgcggcca gcatcgctgc agagaggcgt ggccggttct cgccgatgac
      961 gacgaggtgg gcgccgcgca ccacgccggc gtcggcgagc ccgcacgcga tcgcgcgaac
     1021 gagttgcgcc agctccgccc aggagagcgt ctgccagatc ccgaactcct tctcgcgcag
     1081 ggccgcggcg ccgggccgct cggccgcgtg ctggagcaag agcttgggaa aggtcgtttg
     1141 cactggcgat gcggcacgcc gatgtggcgc gtggtgcggg ctggcccgac tctaggaaga
     1201 ttttgacgcc cggttgtcgt tctgccgaca atctagggga cagtaccctc gggtctatcc
     1261 cttggtcctg ggccgagaaa gaaggtcgcg ccgagcctcg gcgcggatca ggcgaggtgc
     1321 ccggcgagga cgcgcgccac gtgcagcgct tcgcgaccgg caccgtcggc gatctgctgc
     1381 cggcagctcg tcccgtcggc gacgatccag gcgtcgcccg gcgcgccacg cacggcaggc
     1441 aggagactca gctcggccat ctgcatcgag acggcgtggt gctcggcctc gtagccgaag
     1501 ctgcccgcca tgccgcagca cgagctctcg atgagcgatg gccgcgcgcc cgggatcagg
     1561 cgcagtgcct ccagcacggc gggcatcgca ccgaaggcct tctggtggca atgaccgtgc
     1621 acgacgatcg gccgatcggc ggtcttcagg tgcagcgcca ttcgtccggc gtggctctcg
     1681 cgagtgatga actcctcgaa cagcatcgcc tgcgcggcga cccgggtggc cggcgcgccc
     1741 agccccatca cgagcatctc gtcacgcaag gtgaagaggc atgacggctc gaggccgacg
     1801 atgccgatgc cgcgttcggc gaacggcagc aacgcggcaa ggagcgcctt ggccttggct
     1861 cgcgcccgct ccggcatgcc ggcggccaga taggtccggc cgcagcaaag gggtcggccg
     1921 tcgtcggcgc gcgccacgtg caccgtgtag cccgctgctc gcagcacgcg caccgcggcg
     1981 agcgcgttct cggtctcgaa atgaccgttg aaggtgtcga cgaatagcac cacggccttg
     2041 gtcgcggcga gggtggcgtc gcgatcggcc aggccgagcg acgcggcgac gcgagcgaag
     2101 gtgtcgcgac gccagcgcgg cagcggtcgc ttcgccgaga ggcccaccga gcgttcgacc
     2161 agccggcgca gccaccgcga ccggccgggg aggttggcca gcgaggcgat gcggctgagc
     2221 ctcggtgcga ggtcgggcaa cgtgccgatc aggcggtcgc gcagcgagtg gccgcgctcg
     2281 gccttgcgct gcgccgcgac ctcgaacttc attcgcgcca tgtcgacgcc ggtcgggcag
     2341 tcgcgcttgc agcccttgca gccgacgcac aggtcgagcg cctcggcgac ggcgtcggcc
     2401 gccagcggcg ccgcttcgtc gacacccgcg agctggcccg acagcgccag ccgcaaggtg
     2461 ttggcgcggc cgcgtgtcac gtgctgctcg tcgcgcgtga cgcgatagct cggacacatc
     2521 gtgccggcgt cgaacttgcg gcagtggccg ttgttgttgc acatctcgac cgccttggcg
     2581 agacctcccg acggatcgcc gccgctgccg ggcggcgtcg tcgcctcggt gagcggatcg
     2641 ttctggacgt tccacgccga ccagtcgaag accggcttca gggccaccgt gcggtagccc
     2701 ggcggatggc gaaacagccg cgcgtcgtcc atgcgcggcg gatcgacgat gcggttcggg
     2761 cagaagagtc cgat
//
LOCUS       DASRWS010000102         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2631936, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000102 DASRWS010000000
VERSION     DASRWS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631936"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1073)
                     /gene="egtD"
                     /locus_tag="VFF72_01830"
     CDS             complement(111..1073)
                     /gene="egtD"
                     /locus_tag="VFF72_01830"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZV91923.1"
                     /translation="MADSKTPRFLQTWTADSAAERDSAIAGLLADPPRIAPKFFYDRL
                     GSTLFEAITELAEYYPTRTESAIFASHAADMARVVGSGMSLIDLGAGNCAKAAGLFPL
                     LEPSRYVAVDISVEFLREALRRLQREHPLLDIVGLGQDFSVGIDLPADVLTGPRPVFF
                     YPGSSIGNFDPAEALVFLRRIRTRAAGGGLVIGVDLVKPRAVLEAAYDDALGVTAAFN
                     LNVLRHLDATIGADFSPRQWRHVACFDDARSRIEMHLEARDAVTVTWPGGRREFAAGA
                     RIHTENSYKYTVDRFSAMLGEAGFSSSRYWTDARGWFAVFWAAA"
     gene            1284..1706
                     /locus_tag="VFF72_01835"
     CDS             1284..1706
                     /locus_tag="VFF72_01835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZV91924.1"
                     /translation="MPALDLADLDGHRWRLSELAGKVVLLNFWATWCEPCRAEIPSLD
                     ALARQERSRGLVILAVNYRETATVIRGFLERTPYRATVLLDADGDAASEWTPRVFPTT
                     VLVDRLGRPVSSVLGDLDWRGAQARDLVDPLLGRGAHA"
     gene            1723..>2552
                     /gene="coaBC"
                     /locus_tag="VFF72_01840"
     CDS             1723..>2552
                     /gene="coaBC"
                     /locus_tag="VFF72_01840"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZV91925.1"
                     /translation="MATVPASTSHDLASPGDETSSNKDLAGRHIVVGLSGGIACYKAA
                     ELVRELGRAGASVQVVMSEAAERFVTPVTMQALSNRPVFTSQWDAREANNMAHINLTR
                     EADALLVAPASADFIAAIAHGRADDLLRLMCLARPVASCPLIVAPAMNREMWAHPATA
                     RNVAQIVADGAHVLGPDRGDQACGETGDGRMLEPADLRDELIAFFQPKRLAGRRVLVT
                     AGPTFEPLDPVRGLTNRSSGKMGFAIARAAAEAGAEVTLVAGPVALATPRRVRRVDVT
                     T"
BASE COUNT          387 a          858 c          928 g          379 t
ORIGIN      
        1 gcgtgcatgt gggcggcaac gccgcggcgc gcaaggcatc gcaggcgcgg ttgctcaagt
       61 tcctcgaagg acgttgagcc ttcggggtcg cgacggacgc cgccgacgac tcaagcggct
      121 gcccagaaga ccgcgaacca gcccctggcg tcggtccagt agcgcgagct cgagaagccg
      181 gcttcgccga gcatggcgga gaagcgatcg acggtgtact tgtacgagtt ctcggtgtgg
      241 atgcgcgcgc ccgcggcgaa ctcgcgccgc cctcccggcc aggtcacggt caccgcgtcg
      301 cgtgcctcga ggtgcatctc gatgcgggaa cgcgcatcgt cgaagcaggc gacgtggcgc
      361 cactgccggg gcgagaagtc ggcgccgatc gtcgcatcga gatggcgaag gacgttgaga
      421 ttgaacgctg ccgtcacgcc gagcgcgtcg tcgtaggcgg cctcgagaac ggcacggggc
      481 ttgacgaggt cgacgccgat gacgaggccg ccgcccgcgg cacgggtccg gatccggcgc
      541 aggaagacga gtgcttcggc gggatcgaaa ttgccgatgc tcgagcccgg gtagaagaag
      601 acgggccgcg ggccggtgag cacatcggcc ggaaggtcga tgccgacgga aaaatcctga
      661 ccgaggccga cgatgtcgag cagcgggtgc tcgcgttgca gccggcgcag tgcctcgcgc
      721 agaaattcga ctgatatgtc gacggcgaca tatcgagacg gctcgagcag cgggaagagg
      781 cccgcggcct tggcgcaatt gcccgcgccg agatcgatga gcgacatgcc ggacccgacc
      841 acccgcgcca tgtcggcagc gtggctcgcg aagatggcgg actccgtccg ggtggggtag
      901 tactcagcaa gctcggtgat cgcctcgaac agggtcgagc cgagccggtc gtagaagaac
      961 ttgggcgcga tgcgcggcgg atcggcgaga aggccggcga tcgccgaatc gcgttcggcg
     1021 gccgagtcgg cggtccaggt ctgcaggaag cgtggggttt tcgaatcggc catgaaggtt
     1081 cctcggcgtg ggcgacgccc ggtgcgctcg cgcaccttag cgcggtatcg cccacaatgc
     1141 gtgtcgtgag tgattcgtcg ccctgtcaat cgccgggccg gcgccgcgtc gtggcgatgg
     1201 ggcttttcgc gggtgccgcg gcactgcttg ccggccatgc ttctgccgcc gcggtggttc
     1261 ggccgtggcc ggcggcaaga cccgtgcctg cgctcgatct ggccgatctc gacggccatc
     1321 gctggcgact ctccgaactc gcaggcaagg tcgtcctcct caacttctgg gcgacctggt
     1381 gcgagccgtg tcgcgccgag atcccgtcgc tcgatgcgct ggcaaggcag gagcgcagcc
     1441 gcggcctggt gattctcgcc gtcaactatc gcgaaacggc gacggtgatc cgcggcttcc
     1501 tggagcggac gccgtatcga gccaccgtcc tcctcgacgc cgacggcgac gcggcgagcg
     1561 aatggacgcc acgggtcttt ccaaccaccg tcctcgtcga tcgcctcggc cgtccggtct
     1621 cgtccgtgct gggcgatctc gattggcgcg gcgcacaggc gcgcgacttg gtcgacccgt
     1681 tgctcggccg cggcgcgcac gcctgagtcg accccgtcca ccatggcaac ggtcccggca
     1741 tcgacttcgc acgatctggc gtcccctggc gacgaaacgt cgagcaacaa ggacctcgca
     1801 ggccggcaca tcgtcgtcgg cctgtccggc ggcatcgcct gctacaaggc ggccgagctc
     1861 gttcgcgagc tgggtcgggc cggcgccagc gtgcaggtcg tgatgagcga ggcggccgag
     1921 cgcttcgtca cgccggtgac gatgcaggcg ctttcgaacc ggccggtctt cacgagccag
     1981 tgggatgcgc gcgaggccaa caacatggcg cacatcaacc tgacgcgcga ggccgacgcg
     2041 ctgctcgtgg caccggcgag cgccgatttc atcgccgcca tcgcgcacgg acgtgccgac
     2101 gacctgctgc gcctgatgtg cctggcgcgg ccggtcgcga gctgtccgct gatcgtcgcg
     2161 ccggcgatga accgggagat gtgggcgcat ccggccacgg cgcgcaatgt cgcgcagatc
     2221 gtggccgacg gtgcccacgt gctcggcccc gaccggggcg accaggcctg cggcgagacc
     2281 ggcgacgggc gcatgctcga gcccgccgac ctgcgcgacg agctgatcgc cttcttccag
     2341 ccgaagcggc tggccggcag gcgcgtgctc gtcaccgccg gcccgacttt cgagccgctc
     2401 gacccggtgc gcggcctcac caatcgatcg agcggcaaga tgggtttcgc gatcgcccgc
     2461 gccgcggccg aggccggcgc cgaggtgaca ttggtcgccg gtccggtcgc gctggcgacg
     2521 ccgcgtcgcg tgcggcgagt cgatgtcacg ac
//
LOCUS       DASRWS010000103         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2056403, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000103 DASRWS010000000
VERSION     DASRWS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056403"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1676
                     /locus_tag="VFF72_01845"
     CDS             102..1676
                     /locus_tag="VFF72_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV91926.1"
                     /translation="MTGPPVRGAAPRALFDRAARLAAVTVLGFASGLPLALTGQAMQA
                     WLGADGVDVATIGFLSLVGLPYTFKFLWAPLMDRFEPPWLGRRRGWLVLTQLALAAAL
                     FAMATSPPAASLAAFALLAGLVAWLSASQDVVVDAYRTDLLDASERGLGASLAVLGYR
                     LAMIVSGGVAFIWVDPVQGSGWSWPEVYRTMAIFMLIAAAFSAVLAPRLVRAAAPASV
                     ARNDVLGFVAVVAAVAAGLLATRFVFSPLARALVLPLFSGSAIAIPLQDRWADLVALL
                     VGLVFTVPLAAWAARRSRFETLLGSLASYFDRPGAAAFLAFIVLYKLGDAFAGSLLTP
                     FLLQAMHYSTAEVGVVNKVIGLWLTIGGALLAGAVMMRLGLWRSLLAFGVLQMLGNLG
                     FWWLAVHGRGALPGVVIPPFDWGFVRLAAATPVDGGLLMAIASENITSGMGTAAFVAF
                     LMSLCSRRYSATQFALLSALASIGRVWVGPLAGVLAQSIGWPSFFLVSTVAALPALIL
                     LGVLRGPIRQLDEAPA"
     gene            complement(1720..2385)
                     /locus_tag="VFF72_01850"
     CDS             complement(1720..2385)
                     /locus_tag="VFF72_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV91927.1"
                     /translation="MDLTSIEIETAPDPTSSIVILHGLGADGNDFVPIANELDLSAIG
                     PVRFLFPHAPTRPVTINGGHVMRAWYDILGLDRSEMREDESGLRASQALVEALIAKEK
                     SRGVPARRIVLAGFSQGCAMTLMAGLRHDERLAGLAGLSGYLPLAAKAAAERHAANHD
                     VPIFLAHGTQDPVIPIARARDSRDTLSRLGYAVEWHEYPMPHSVSAAEIADLNRWLVA
                     ALT"
     gene            complement(2393..>3057)
                     /locus_tag="VFF72_01855"
     CDS             complement(2393..>3057)
                     /locus_tag="VFF72_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZV91928.1"
                     /translation="GRFDVVAGMKGHFHFAEKKGYSGVGLYARRKPSAVRPGFGEREF
                     DAEGRYVEARFDRPGRRKLPKLSIVSCYFPSGSSGEHRQAAKFRFLDCFAPYLEKLRR
                     EREVILVGDVNIAHKEIDLKNWKGNRKNSGFLPEERAWMTRLLEEIGFVDVFRTLNPH
                     PEQYTWWSNRGQARAKNVGWRLDYHLATPTVAALARSEHIYLERRFSDHAPLVIDYDF
                     NL"
BASE COUNT          382 a         1033 c         1066 g          576 t
ORIGIN      
        1 aacatcgcaa gcgcggcgcg aaaggcgtcg tcggcaaagc tcggcgaagc ggcccggggg
       61 aatcggctca tgatgcattg tcggtcccta gaatgccttg catgaccgga ccgcccgtgc
      121 gaggcgccgc tccccgcgcg ctcttcgatc gggccgcccg gctcgccgcg gtgacggtgc
      181 tcggattcgc ctcggggctg ccactggcac tcaccggcca ggcgatgcag gcgtggttgg
      241 gcgccgacgg cgtcgacgtg gcgacgatcg gcttcctgag cctggtcggc ttgccctata
      301 cgttcaagtt cctgtgggcg ccactgatgg atcgcttcga gccgccctgg ctgggccgtc
      361 gtcggggctg gctggtcctg acccagcttg cgctcgcggc cgcgttgttc gccatggcga
      421 cgtcgccgcc ggcggcgtcg cttgccgcgt tcgcgcttct ggccggactc gtcgcctggt
      481 tgtcggcctc gcaggatgtc gtcgtcgacg cctatcgcac cgacctgctc gatgcgagcg
      541 agcgcggcct cggcgcttcg ctcgcggtgc tcggctaccg gctcgcgatg atcgtctccg
      601 gcggcgtcgc cttcatctgg gtcgatccgg tgcagggcag cggctggagc tggccggagg
      661 tctaccggac gatggcgatc ttcatgctga tcgccgcggc gttttccgct gtgctcgcgc
      721 ccaggctggt tcgcgccgcg gcgccagcca gcgtcgcccg caacgatgtt ctcggcttcg
      781 tcgccgtcgt cgctgccgtc gccgccggat tgctggcgac gcgcttcgtc ttttcgccgc
      841 ttgcccgcgc gctggtcctg ccgctctttt ccggcagcgc gatcgcaatc ccgctgcagg
      901 atcgctgggc cgatctcgtg gcgttgctgg tcggccttgt cttcacggtg ccgctggccg
      961 cgtgggccgc gcggcgaagc cggttcgaga cgcttctggg cagcctcgcg agctatttcg
     1021 atcgccccgg ggcggccgcg ttcctcgcct tcatcgtcct ctacaagctg ggtgacgcct
     1081 tcgccggctc gttgctgacc ccgttcctgc tgcaggcgat gcactacagc acggccgagg
     1141 tcggcgtcgt caacaaggtg atcggcctgt ggctcacgat cggcggcgcg ctgctggccg
     1201 gcgcggtgat gatgcgactg gggctctggc gctcgctgct ggcgttcggc gtgttgcaga
     1261 tgctgggcaa cctcggcttc tggtggcttg ccgtgcatgg tcggggcgcg ttgcccggcg
     1321 tcgtcatccc gcccttcgac tggggattcg tccgcctcgc tgccgcgacg ccagtggacg
     1381 gcggcctgct gatggcgatc gccagcgaga acatcaccag cggcatggga accgcggcct
     1441 tcgtcgcctt cctgatgagc ctgtgcagcc gtcgctacag cgcgacgcag ttcgcgctgc
     1501 tgagtgccct ggcatcgatc ggccgggtct gggtcggccc gctcgccggc gtcctcgcgc
     1561 agtcgatcgg ctggccgtct ttctttctcg tctcgaccgt ggcggcgttg cccgcgctga
     1621 tcttgctggg ggtcttgcgc ggcccgatca ggcaactcga cgaagccccg gcctgaagca
     1681 cggcgaccgc ctttggcgtg ccggcttcgg cgcggtgcct caggtcagcg ccgcgaccag
     1741 ccagcgattg aggtcggcga tttccgcggc cgagaccgaa tgcggcatgg ggtattcgtg
     1801 ccattccacg gcatagccga gacgcgagag cgtgtcgcgc gaatcgcggg cccgggcgat
     1861 cgggatcacg ggatcctgcg tgccgtgagc gaggaagatc ggcacgtcgt ggttggcggc
     1921 gtgacgttcg gcggcagcct tggcggccag cggcagatag ccggacagtc ccgccagccc
     1981 ggccagacgc tcgtcgtgac gcaggcccgc catcagcgtc atcgcgcagc cctggctgaa
     2041 gccggcgagg acgatgcgtc gggccggcac gccgcggctt ttctccttcg cgatcagcgc
     2101 ctcgaccagc gcctgggacg cccgcagccc ggactcgtcc tcgcgcatct cgctgcggtc
     2161 gaggcccagg atgtcgtacc aggcgcgcat gacgtggccg ccgttgatcg tcaccggccg
     2221 ggtcggcgca tgcggaaaga gaaatcgcac cggcccgatc gcgctcaggt cgagctcgtt
     2281 cgcgatcggc acgaagtcgt tgccgtcggc gcccaggccg tgcaggatga cgatgctcga
     2341 cgtcggatcg ggcgcggttt cgatttcgat gctggtcagg tccatggtcc ggtcagagat
     2401 tgaagtcgta gtcgatgacg agcggcgcat gatcgctgaa gcgccgctcg aggtagatgt
     2461 gctcgcttcg cgccagggcg gccaccgtcg gcgtcgcgag gtggtagtcg agtcgccagc
     2521 cgacgttctt cgcccgtgcc tggccgcggt tactccacca ggtgtactgt tcggggtgcg
     2581 gattgagcgt gcgaaagacg tcgacgaagc cgatctcctc gagcagtcga gtcatccagg
     2641 cgcgctcctc tggaagaaac ccgctgttct tgcggttgcc cttccagttc ttcaggtcga
     2701 tttccttgtg cgcgatgttg acgtcgccga cgaggatcac ctcgcgttcc cggcgaagtt
     2761 tctcgaggta aggcgcgaag caatcgagaa agcggaattt cgccgcctgc cgatgctcgc
     2821 ccgacgagcc gctcgggaaa tagcagctga cgatgctgag cttgggcagc tttcgccggc
     2881 ccggccggtc gaagcgggcc tcgacatagc gcccctcggc atcgaactcg cgctcgccga
     2941 agccgggccg caccgcgctc ggcttgcgtc gcgcgtagag gccgaccccg gaatagcctt
     3001 tcttctcggc gaaatggaag tgccccttca tcccggccac gacgtcgaag cggccgg
//
LOCUS       DASRWS010000104         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1766793, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000104 DASRWS010000000
VERSION     DASRWS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766793"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFF72_01860"
     CDS             complement(<1..549)
                     /locus_tag="VFF72_01860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV91929.1"
                     /translation="MARTQPGRRAAAAALSARRQRHLTRRHAPHSRMNSFVLHRIGAL
                     DLDRAARLHRDAFAPIGERPWTRQDMAELLATPAGGGLILQIDDEDAGVVLWRTIADE
                     AELLTLAVDARHRRRGVGAALLGQVIVRSRRQGARQLFLEVGVDNPGARSLYSQAGFI
                     EVGRRVAYYKRPTGFADALVLRL"
     gene            complement(450..1136)
                     /gene="tsaB"
                     /locus_tag="VFF72_01865"
     CDS             complement(450..1136)
                     /gene="tsaB"
                     /locus_tag="VFF72_01865"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZV91930.1"
                     /translation="MPSRFPERQRMTVLAFDCAISGLAVAVARDGVGLAERREEGRGQ
                     AATLLPAIAATLEEAGIDRRALSLIAVTVGPGSFTGVRVGLAAARGLALALGVPLAGI
                     TTTAALLAAAPHDDRVSVAAIDTHLGDWFCALDGQPPFVATTDALVAKLAGQPCRIVG
                     PQADALARQVPNTIAQQVLPDPVLLARLAMADGIEGWRARNRAEGLPRPLYLRGVNVT
                     SPDGTRRTVE"
     gene            1146..1700
                     /gene="ddpX"
                     /locus_tag="VFF72_01870"
     CDS             1146..1700
                     /gene="ddpX"
                     /locus_tag="VFF72_01870"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HZV91931.1"
                     /translation="MKFDLVEIAPPVFDVDVTLAYATADNLTGQPVYRKAECFLHRDA
                     ADKLRAAIDLARSLGLRLRIFDALRPVEAQWKLWNFNPDPEFLADPRRGSPHSRGVAV
                     DLTLLDGGRALDMGTGFDAFTPLSHHGRTDVSQEAQRNRLLLMGLMTTAGWDFYRNEW
                     WHYQLFDARRYALVSDADLPRPMM"
     gene            complement(1746..>2281)
                     /locus_tag="VFF72_01875"
     CDS             complement(1746..>2281)
                     /locus_tag="VFF72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HZV91932.1"
                     /translation="YLRDKIANETGLSPIERIWLEQLADRYDTPVDKLDELVKRVDIV
                     PPSMAIAQSGIESGWGTSFAARNGNALFGQIQSTGRHAVSVPWKPGSGMPQPFTNVGE
                     AADAYVSNLNTHPAYAAFRNERAAMRQRGEAPDGYRLVGQLVKYSELGQQYVGFVRQV
                     MREDKLTDFDKAKLSAF"
BASE COUNT          361 a          777 c          782 g          361 t
ORIGIN      
        1 gagacgcagg acgagcgcat cggcaaaacc cgtcggccgt ttgtagtagg ccacgcgtcg
       61 tccgacttcg atgaagcccg cctgtgaata aagcgagcgc gcgcccggat tgtcgacgcc
      121 gacctcgagg aagagctggc gcgcgccctg ccgacgtgaa cgcacgatca cctgaccgag
      181 caaggccgca ccaacaccgc gccggcgatg cctggcatcg acggcgagag tgagcagttc
      241 cgcctcgtcc gcaatggtcc gccacagcac gacgccggcg tcttcgtcat cgatctgcag
      301 gatcagccca ccgcccgccg gcgtggccag caattccgcc atgtcctggc gcgtccatgg
      361 ccgctcgcct attggcgcga acgcgtcgcg atgcagccgg gcagcccgat cgaggtcgag
      421 cgcgccgatt cgatgaagca cgaagctgtt cattcgactg tgcggcgcgt gccgtcgggt
      481 gaggtgacgt tgacgccgcg cagatagagc ggccgcggca gcccttcggc ccggttgcgt
      541 gcgcgccatc cctcgatacc gtcggccatc gcgagccgcg ccagcaggac cgggtccggc
      601 agcacttgtt gggcaatcgt attgggcacc tgtcgcgcca aggcgtcggc ctgaggcccg
      661 acgattcggc acggttgtcc ggcgagcttg gcgaccaacg cgtcggtcgt ggcgacgaac
      721 ggaggctgcc cgtccaaggc gcagaaccag tcgccgagat gcgtgtcgat ggcggccacc
      781 gagacacgat cgtcgtgcgg tgccgcggcc agcaacgccg ccgtcgtggt gatgccggcg
      841 agcggcacac cgagcgccag cgccaacccg cgcgcggcag cgagtccgac gcgcaccccg
      901 gtgaagctgc cggggccgac cgtgaccgca atcagcgaca gtgcccggcg atcgatgccc
      961 gcttcctcca gggtcgcggc gatcgccggc agcagcgtcg ctgcctggcc gcggccctcc
     1021 tcgcgccgct cggccaggcc gacgccatcg cgcgcgacag cgaccgccag cccgctgatg
     1081 gcgcagtcga aggcgaggac ggtcattcgc tggcgctccg ggaagcggct gggcattgtg
     1141 cagtcatgaa gttcgatttg gttgagatcg ccccgcccgt cttcgatgtc gatgtgacac
     1201 tggcctacgc gaccgccgac aatctaaccg gccagccggt gtaccggaaa gccgaatgct
     1261 tcctgcatcg cgatgccgcc gacaagctgc gggcggcgat cgacctcgcg cgctcgctgg
     1321 gcctgcggct gcgcatcttc gatgcgctgc ggccggtcga ggcgcagtgg aagctgtgga
     1381 acttcaatcc cgatcccgaa ttcctcgccg acccgcggcg cggctcgccg cattcgcgcg
     1441 gcgtggcggt cgacctcacg ctgctcgatg gcgggcgcgc gctcgacatg ggcaccgggt
     1501 tcgacgcctt cacgcccctg tcgcaccatg gccgcaccga cgtttcgcaa gaggcgcagc
     1561 gcaatcgcct gctgctgatg gggttgatga ccacggcggg ctgggacttc tatcgcaacg
     1621 agtggtggca ctatcagctg ttcgacgcgc gacgctatgc gctggtgagc gatgccgatt
     1681 tgccgcgtcc gatgatgtga tcggccacgc tctggccgat catcccgagc gatagcgagg
     1741 gatctctaga aagcggacag cttcgccttg tcgaagtcgg tgagcttgtc ctcgcgcatc
     1801 acctggcgca cgaagccgac atactgctgg ccgagctcgg agtacttcac cagttggccg
     1861 accaggcgat agccgtcggg cgcctcgccg cgctggcgca tcgccgcgcg ctcgttgcgg
     1921 aaggcagcgt aggccggatg cgtgttgagg ttggagacgt acgcatcggc cgcctcgccg
     1981 acattggtga agggctgcgg catgccggaa cccggtttcc acggcaccga cacggcatgg
     2041 cgaccggtcg actggatctg gccgaacaac gcgttgccgt tgcgcgcggc gaacgaggtg
     2101 ccccagcccg attcgatgcc gctctgggcg atcgccatcg acggcggcac gatgtcgacg
     2161 cgcttcacca gctcgtcgag cttgtcgacc ggcgtgtcgt agcggtcggc gagctgctcg
     2221 agccagatgc gctcgatcgg cgagaggccc gtctcgttgg cgatcttgtc gcgcaggtag
     2281 a
//
LOCUS       DASRWS010000105         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2632599, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000105 DASRWS010000000
VERSION     DASRWS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632599"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFF72_01880"
     CDS             complement(<1..587)
                     /locus_tag="VFF72_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZV91933.1"
                     /translation="MTPAAEEKSGGVAAVGRALAVIKAFRAGDGSLTLNELAHRTGLY
                     KSTILRLVVTLAREHCIVRLDDGTYQLGSMLLHWGGIYQSALRMDDHVPMVLRRLAQE
                     TGEGASFFTREGELRVCLFRVDSPRSIRDHIHVGDLLPLDKGAAGRVLASFDSALVPR
                     ESFPRQVLIATVGEREPDIAAIAAPVFGPHGSLRGA"
     gene            765..3068
                     /locus_tag="VFF72_01885"
     CDS             765..3068
                     /locus_tag="VFF72_01885"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZV91934.1"
                     /translation="MSSRDHLPLSEEPRADYLAKVTGAAVFASDVEVPGMLHGKVLRS
                     PLPHARIRKIDTSAALSIPGVVAVLTGDDLRDLPGGVDRWGLYLRDRPVIALEKVRYV
                     GDPVAAVAAVDEATAEDALDAIVVDYDPLPHVTTSEEALRGEAALVHEEADKLKDFYF
                     RGEASPVAGTNVFQHWRYASGDVEQAFASAERVFEDDFTFPMVFHYAMEPHVCVAHWA
                     PHSLQLWSGGQTPSAIQRVCSDLFGIPLACVRVHSPYVGGGFGGKASVKIDPLVAALS
                     WKARRPVRVCLSIAESMLTCRRLSAEITLKTAVDREGRITAKSVRAVLNGGAYADTGP
                     AIAIKAAIRAIGPYHIPNLRLEAIGVYTNTVPGAAFRSIGGPQGVWASESQMDIMATE
                     LGLDPVAFRMANLADKGQTIKPDLRPIDVDMRESLRQAIDAFGGLPEPKSLLRRARGV
                     AVAASDPGIMPMGGALVRLRADGSVNISANTVELGQGSRGVLRLIAAKTLRQPLRFIA
                     VAEPDTAQAPYDWGTGASRSTVIIGLAVQLACEDVLRQVFETAAHVQGGVVSDYILTE
                     RGVESAAGALPLRELLRRLNGIDAGEFLGVGRVSSMTKIGNFDFKLAPLFWETASSVC
                     EIDLDEDTGAIHVLRVTGAADLGQVINRKAAEGQDEGAMVMGIGHTLFEEYVYEDGQV
                     VNGTMFDYHVPTLEDSPLHVHTVLLESGDGPGPGGARGGGEGAILPVAPAIANALFQG
                     WRVRVKELPLTAERVWRALQLARTGSR"
     gene            3083..>3459
                     /locus_tag="VFF72_01890"
     CDS             3083..>3459
                     /locus_tag="VFF72_01890"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZV91935.1"
                     /translation="MDFRIEAQLPATAAQLWAVFFDVHRVAGLIPGCEAVEEVEPLKA
                     YSAVMKQRIGIFKLEVPTRITVERSTFEREVALHAMGRDKLTATLIDMHLTVVLDERA
                     DAVPPACGIALEAHLQVAGRLASL"
BASE COUNT          568 a         1128 c         1159 g          604 t
ORIGIN      
        1 gcgccgcgta ggctgccgtg cgggccgaag acgggcgcgg caatggcggc gatatcgggc
       61 tcgcgctcgc cgacggtcgc gatgagcacc tgacgcggaa agctctctcg aggcacgagt
      121 gccgagtcga agctggcgag gacacgcccg gccgccccct tgtccagggg caagaggtcg
      181 ccaacgtgga tgtggtcgcg gatcgatctc ggcgagtcga cacggaacag gcagacgcgc
      241 agctctcctt cgcgcgtgaa gaacgaggcg ccttcgccgg tctcctgcgc cagccgtcgc
      301 aggaccatcg gcacgtggtc gtccatgcgc agggccgact ggtagatgcc gccccaatgc
      361 aacagcatcg agccgagttg gtaagtgccg tcgtcgagcc tgacgatgca gtgctcacgc
      421 gcgagcgtga ccacgagccg caggatggtg cttttgtaga gccccgtccg atgggcgagc
      481 tcattcaggg tcagcgagcc gtctcctgcc cggaacgcct tgatgacggc gagcgcgcgc
      541 ccgaccgcgg ctacgccgcc gctcttttcc tcagctgccg gggtcatttc cgccttgcga
      601 gatttccctc ttgacattat ctcgtgctga ccaacagaat caagttcttt ctagtagaac
      661 ttgctgagca agccacttca cttcggagca gcccaggcga atgcttgacg aatgcgaagc
      721 agccgaacgg aatcgactcg cgcccaagcg ctgtccaggt cgcgatgtcg tcgcgtgacc
      781 acttgccgct gagcgaagag ccgcgcgccg actacctggc gaaggtgacc ggcgcggccg
      841 tgttcgcgag tgacgtcgaa gtgccgggca tgctgcatgg aaaggtgctt cgcagcccgt
      901 tgccgcacgc ccgcatcagg aagatcgaca ccagcgccgc cctgtcgatt cccggcgtgg
      961 tcgcggtgct gactggagac gatctccgcg acctgcctgg aggcgtcgat cgctggggcc
     1021 tgtatcttcg cgaccgtccg gtcatcgctt tggagaaggt gcgctacgtc ggcgacccgg
     1081 ttgccgcggt cgccgccgtc gacgaggcaa cggccgagga tgcgctcgat gcaatcgtcg
     1141 tcgactacga cccgctgccg cacgtcacga cttccgaaga ggcgttgcgc ggggaagccg
     1201 ctctcgtcca cgaggaagcg gacaagctga aggacttcta cttccgcggc gaagcatcgc
     1261 ccgtggcggg gaccaacgta ttccagcact ggaggtatgc ctcgggcgac gtcgagcagg
     1321 ccttcgcttc agcggagcgc gtcttcgaag acgacttcac cttcccgatg gtgtttcact
     1381 acgcgatgga gccgcacgtc tgcgtggccc actgggcgcc gcactcgctt cagctctgga
     1441 gcgggggcca gacgccgagt gccatccagc gggtctgctc ggatctgttc ggcatcccgc
     1501 tggcctgcgt ccgggttcat tcgccctatg tcggaggcgg cttcggcggc aaggcgtcgg
     1561 tcaagatcga cccattggtc gcggcgctgt cgtggaaggc ccgtcggccg gtccgggtgt
     1621 gcctgtcgat cgccgagtcg atgctgacgt gccggcgcct gagcgccgag atcacgctca
     1681 agacggcagt cgaccgggaa gggcgtatca cggcgaaatc cgtccgagcc gtcctcaacg
     1741 gcggcgccta cgccgatacc gggccggcga tcgcgatcaa ggcggcgatt cgtgcgatcg
     1801 gcccgtatca catccccaac ctgcgtctcg aagcgatcgg cgtctatacg aacaccgtcc
     1861 caggcgcggc ctttcgctcg attggcgggc cgcagggtgt gtgggcctcc gaatcgcaga
     1921 tggacatcat ggccaccgag ctcggtctcg acccggtcgc gtttcgcatg gccaaccttg
     1981 ccgacaaggg ccagaccatc aagcccgacc tgcgcccgat tgatgtcgac atgcgtgaat
     2041 cgctgcgcca agccatcgat gcgttcggtg gtttgcccga gccgaagtct ttgcttcgcc
     2101 gtgcccgagg cgtcgccgtc gcggccagcg atcccggcat catgcccatg ggcggtgcgc
     2161 tcgttcgtct gcgcgccgac ggctcggtca acatttctgc caacaccgtc gaactggggc
     2221 agggcagtcg cggggtgctg cgcttgatcg ccgcgaagac gctgcgccaa ccacttcgct
     2281 tcatcgccgt cgccgagccc gataccgctc aggcgccgta cgactggggc accggcgcga
     2341 gccgctcgac ggtgatcatc ggcctggccg tgcagctggc ttgcgaagac gttctgcgac
     2401 aagtgttcga aacggccgcg catgtccagg gcggcgtcgt ctcggactac atcctgacgg
     2461 agcgtggcgt cgaaagcgcc gcgggcgcgc tgcctttgcg cgaactgctg cgccgtctca
     2521 acggcataga cgccggcgag ttcctcggcg tcggacgcgt ttcgtcgatg acgaagattg
     2581 gcaatttcga tttcaagctc gcgccgctct tctgggagac ggcctccagc gtctgcgaga
     2641 tcgacctcga cgaagacaca ggcgcaatcc acgtcctgcg cgtgacgggt gccgccgacc
     2701 tcggccaagt catcaatcgc aaggccgctg aagggcagga cgaaggcgcg atggttatgg
     2761 gcatcggcca tacgctcttc gaggaatacg tctacgagga tggccaggtc gtcaacggca
     2821 cgatgttcga ctaccacgtg ccgaccctgg aggacagtcc actgcacgtc cataccgtcc
     2881 tgctcgaaag cggcgacggg ccggggccgg gcggcgcgcg tggcggcggc gagggcgcga
     2941 tcctgccggt tgcgccggcc atcgcgaacg ccttgttcca gggctggcgc gtgcgcgtga
     3001 aggagttgcc gctgaccgcc gagcgtgtct ggcgggcgtt gcagctcgcc cgcaccggtt
     3061 cccgataagg cgccggcaga ccatggattt caggatcgaa gcacagctgc cggcaacggc
     3121 tgcacagctg tgggcggtct tcttcgatgt tcaccgcgtc gccggcctta ttccgggctg
     3181 cgaggctgtc gaggaagtcg agccgctcaa agcgtactcg gctgtgatga aacagcgtat
     3241 cggcattttc aagttggaag ttccgacgcg catcaccgtc gagcgttcga cattcgagcg
     3301 tgaggttgcg ctgcacgcca tggggcgcga caagctcacg gcaacgctga tcgacatgca
     3361 cctgacagtc gtccttgatg agcgagccga cgccgtgccg ccggcttgcg gcattgcgct
     3421 cgaggcgcac ctgcaggtgg cgggccggct cgcgtcgct
//
LOCUS       DASRWS010000106         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3782970, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000106 DASRWS010000000
VERSION     DASRWS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782970"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VFF72_01895"
     CDS             <1..176
                     /locus_tag="VFF72_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HZV91936.1"
                     /translation="TTFLPSSVYLISRMPSDPQRGDIVPIVVISLVLAFVATLYPSWR
                     ASRLQPAEALRYE"
     gene            169..888
                     /locus_tag="VFF72_01900"
     CDS             169..888
                     /locus_tag="VFF72_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV91937.1"
                     /translation="MSEAPTAEISADGPVVVAQALHKRFVEGSGASRLDVHVLRGVDL
                     AVAAGETLAVVGASGSGKSTLLHLLGGLEAPTSGTVRLAGRDLATLGAAAQGEWRNRQ
                     LGFVYQFHHLLPEFSALDNVAMPLRIRRESNEAARRSAAETLAQVGLADRIAHRPAQL
                     SGGERQRVAIARALAGAPACVLADEPTGNLDRVTADKVFALLLDLARRRAMALVLVTH
                     DETLAARCDRTLHLVQGKLAG"
     gene            complement(1026..1388)
                     /locus_tag="VFF72_01905"
     CDS             complement(1026..1388)
                     /locus_tag="VFF72_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91938.1"
                     /translation="MRHTALLAVSLALSLFAGEAAARAYDAKALARYDTSYQKCESRF
                     TEMRGHRDEAYLNLWRIELTPKTQAQLAKLRASPTYRSEQQRLAGTPASKTTAAAAPV
                     ERECQGLWGELQRRKPKH"
     gene            complement(1657..1869)
                     /locus_tag="VFF72_01910"
     CDS             complement(1657..1869)
                     /locus_tag="VFF72_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91939.1"
                     /translation="MKIVGIILIVLGALALAYGGFTYTRNSTAVKLGPLELDVKKKET
                     VDVPVWAGIGAIVIGGLVLVMGARKP"
     gene            complement(2057..2257)
                     /locus_tag="VFF72_01915"
     CDS             complement(2057..2257)
                     /locus_tag="VFF72_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91940.1"
                     /translation="METRRIRPQRSVTPIEFESMSDEQLARLLPRALREYFPGKDNCA
                     DGCFYLHDGTAWSFFKGGPLDE"
     gene            complement(2264..>2630)
                     /locus_tag="VFF72_01920"
     CDS             complement(2264..>2630)
                     /locus_tag="VFF72_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZV91941.1"
                     /translation="GSAKCHLVETGRDADLVWGVLYEIDIAEKGRLDEAEGLGVHYAH
                     RNVRVETDAGTVEAGAYFAIDADESLRPFDWYVAFVLAGAAEHGLPADYVAAIAALPV
                     LVDDDAVRRSRNLAILADR"
BASE COUNT          386 a          897 c          928 g          419 t
ORIGIN      
        1 acacgacgtt cctgccgagc agcgtctacc tgatcagtcg catgccgagc gatccgcagc
       61 gcggcgacat cgtccccatc gtcgtcatct cgctcgtcct tgccttcgtc gccacgctct
      121 accccagctg gcgagcgagt cgattgcagc cggccgaggc gttgcgctat gagtgaggcg
      181 ccgacggcgg agatcagcgc cgatgggccg gtcgtcgtcg cccaggcgct gcacaagcgt
      241 ttcgtcgaag gcagcggcgc atcacgcctc gacgtgcacg tgctgcgcgg cgtcgatctg
      301 gcggtcgccg cgggcgagac gctggccgtc gttggcgcgt cgggctcggg caagagcacc
      361 ctgctgcatc tgctcggcgg cctcgaggcg ccgaccagcg gcacggtccg gctggcgggt
      421 cgcgacctgg cgacgctcgg cgccgccgcc caaggcgaat ggcgcaaccg gcagctcggc
      481 ttcgtctacc agtttcatca cctgctgccg gagttctcgg cgctcgacaa cgtggcgatg
      541 ccgctgcgca tccgtcgcga atcgaacgag gccgcgcggc ggtcggccgc cgagaccctg
      601 gcgcaggtcg gcctcgccga ccggatcgcg caccggcctg cgcagctctc cggcggcgag
      661 cgccagcgtg tcgccatcgc ccgggcgctg gccggtgcgc cggcctgcgt gctcgccgac
      721 gagccgaccg gcaacctcga tcgcgtcacc gccgacaagg tcttcgcgct gctgctcgac
      781 ctggcccggc ggcgcgccat ggcgctcgtc ctcgtcacgc atgacgagac gctggcggcg
      841 cgctgcgacc ggacgctgca tctcgtgcag ggaaaactcg ccggctgaac cgcgatcgat
      901 gccatacggg cgcgacgacc atcgaacgag gcatcgtcga gccagcagca ggtcccgccg
      961 ctgcgccaca tcgcctacga attgtgctgg tcgcggagag gacagcgacc ggtgaggccg
     1021 ccgcctcagt gtttcggctt gcggcgctga agctcgcccc acaggccctg gcattcgcgc
     1081 tcgaccggcg cggcggcagc ggtcgtcttc gaggcgggcg tcccggccag ccgctgttgc
     1141 tcggagcgat acgtcgggct ggctctcagc ttcgccagct gcgcctgcgt tttcggtgtc
     1201 aactcgatgc gccagagatt gaggtaggcc tcgtcgcgat ggccgcgcat ctccgtgaac
     1261 cggctctcgc atttctggta gctggtgtcg tagcgggcga gcgccttggc gtcgtaggca
     1321 cgcgccgcgg cttcgcccgc gaacagcgac agggcaagcg agacggcgag cagggcggtg
     1381 tggcgcatgg gtcgaagcat aggcgatcgg tgcgaaagga gcgaaacgga atgttgcttc
     1441 ggctgcggcc acgcccggcc gttgcagcgt tcaggggtcg tccggaacac gttgcgtctg
     1501 cgtcaagacg gcgcacagcc gcccttcggc tttgcgcacc ggcgagcgcc gtcaggcgaa
     1561 cgcccatccg gcccggaata agcggagcgc gaagcgcttg cagcggattg gcgtcgacgc
     1621 tcgcaacccc ttccggcggc aacgagtccg ccgtgttcag ggcttcctgg cacccatgac
     1681 cagcaccagg ccgccgatga cgatcgcccc gatgcccgcc cagaccggga cgtcgaccgt
     1741 ctccttcttc ttgacgtcga gctcgagcgg cccgagcttc accgcggtcg aattgcgcgt
     1801 gtaggtgaag ccgccgtagg ccagcgccag cgcgccgagg acgatgagaa tgatgccgac
     1861 gatcttcatg gacagccctg tgaatggagg tgcggatgat tctgccgtcc gggtcgcacc
     1921 gggcactgcg ggttggcgat aggtcttcgg gcatgtcggc atgcgaccgg cggccggctt
     1981 ggctgtgcac ctcgggcggg aagcggactc gaggaagcac cttgaacgat ggccggcgct
     2041 cggcgcccgg tgcgcgtcac tcgtcgagag gtccgccttt gaagaagctc cacgcggtgc
     2101 cgtcgtgcag gtagaagcag ccgtcggcgc agttgtcctt gccggggaag tattcgcgca
     2161 gggcgcgcgg cagcaggcgt gcgagctgct cgtcgctcat cgactcgaac tcgatcggcg
     2221 tgaccgaacg ttgcggacgg attcgtcgtg tttccatgag gtttcagcgg tcggccagga
     2281 tcgccaggtt gcggctgcgc cgaaccgcgt cgtcgtcgac gaggaccggc agcgcggcga
     2341 tcgcggcaac gtagtcggcg ggcagtccgt gctcggcggc accggcgagg acgaaggcga
     2401 cgtaccagtc gaacggccgc agcgactcgt cggcatcgat cgcgaaatag gcaccggcct
     2461 cgaccgttcc ggcatcggtc tcgacgcgca cgttccggtg cgcgtagtgc acgcccaggc
     2521 cctcggcctc gtcgagacgg cccttttcgg cgatgtcgat ctcgtacagc acaccccaga
     2581 cgaggtcggc gtcgcggcca gtctcgacga ggtggcactt ggccgatccg
//
LOCUS       DASRWS010000107         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2339973, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000107 DASRWS010000000
VERSION     DASRWS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339973"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFF72_01925"
     CDS             complement(<1..572)
                     /locus_tag="VFF72_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV91942.1"
                     /translation="MKRTHSAVLAAGLAAVALCGAGSAVAQTKTLRFVAHADVKILDP
                     TFTTAYITRNFGYMVYDTLFAQDAKGTPKPQMVEKWTSSKDGKQWSFTLRPGLKFSDG
                     KAVTSADCIASLQRWSGRDSIGRAMNAAGAEWKAVDAKTFTLSLKDPFGLVLEGLGKP
                     SGFPPVILPERIARMPTTSPLTEVVGSGPFL"
     gene            complement(569..2044)
                     /locus_tag="VFF72_01930"
     CDS             complement(569..2044)
                     /locus_tag="VFF72_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZV91943.1"
                     /translation="MRVFTGCLATETNTFAPMPTGLGAFRDRGYYAAGTHPEALSFFA
                     APLWAARQRARERGWTLVEGMVAAAQPGGTTTRAAYELLRDELLGDLRAAMPVDMVVL
                     GLHGAMVADGYDDCEGDLLAKVREIVGPKVVVGAELDPHNHLTPAMLTNADLLISFKE
                     YPHTDVLERAWELVDLCAATVEKRLRPVAAAVDCEMLVTMHTSRQPARAFVDRIQSLE
                     GKDGILSISVSHGFAWGDTEHMGTQILVYADDDAAKAATMARRLADELIGMREQLRIR
                     FPGIDEALDEALAFEGGPVVLSDGADNPGGGAPGDSTFILRRMLERGIGNACLGPLWD
                     PVAAKIAFEAGPGARLALRLGGKIGPLSGDPIDAVCTVKAVLPDMIMTGLAGTPLAMG
                     DSALVETAGIEIVLISLRNQAMGTDLFTQLGCDLATKKIIVVKSSQHFFASFSKVARH
                     VIYVGAPGAVAIDLDTLPYCKIRRPKWPLRDATPPTQETSR"
     gene            2143..2868
                     /locus_tag="VFF72_01935"
     CDS             2143..2868
                     /locus_tag="VFF72_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZV91944.1"
                     /translation="MVFSNAVAPLAMIAATVLAGAFVQGTTGVGFALVVAPVLGLLAP
                     ELLPATVLLLMVPLNVYVVWRERGSIDRLGAAWITGGRLVGTAGGFWVLVALSVGRLS
                     LFVGLATALAVLATLAAPRFTPSRSAFVAAGVVTGITETATGIGGPPLALVYQHHPVA
                     TMRPTLALCFLLGQLMSLATLAIAGRIGSAQWLATAGLVPALALGAWLSHRVHRSVGG
                     RFLRRFVLAFAFVSSLALIAKSL"
BASE COUNT          406 a         1024 c         1059 g          479 t
ORIGIN      
        1 agaaaggggc cggagccgac gacctcggtc agcggcgagg tggtcggcat cctggcgatc
       61 cgctcgggca ggatcacggg cggaaaaccc gacggcttgc cgaggccttc gaggacgagg
      121 ccgaacggat ccttcaggct caaggtgaag gtcttcgcgt cgaccgcctt ccattcggcg
      181 ccggccgcgt tcatcgcccg gccgatgctg tcacgcccgc tccagcgctg cagcgaagcg
      241 atgcagtcgg ccgacgtgac tgccttgcca tcactgaact tgaggccggg gcgcagcgtg
      301 aagctccact gcttgccgtc cttcgagctc gtccacttct cgaccatctg cggcttcggc
      361 gtgcccttcg cgtcctgggc gaacagcgtg tcgtagacca tgtagccgaa gttgcgcgtg
      421 atgtaggccg tcgtgaaggt cgggtcgagg atcttcacgt cggcgtgggc gacgaagcgc
      481 agcgtcttgg tctgcgccac ggcgctgccg gcgccgcaga gcgcaacggc ggccaggccg
      541 gccgcgagca cggcgctgtg ggttcgtttc atcgtgaggt ctcctgggtt ggcggggttg
      601 cgtcgcgcag cggccacttc ggccggcgga tcttgcaata cggcagggtg tcgaggtcga
      661 tcgccacggc gccgggcgcg ccgacgtaga tgacgtgcct cgcgaccttc gagaacgagg
      721 cgaagaaatg ctgcgacgac ttgacgacga tgatcttctt cgtcgccagg tcgcagccga
      781 gctgcgtgaa gaggtcggtg cccatcgcct ggttgcgcag gctgatgagg acgatctcga
      841 tgcccgccgt ctcgaccaag gccgaatcgc ccatcgccag cggtgttccg gcgagcccgg
      901 tcatgatcat gtcgggcagc acggccttga cggtgcacac cgcgtcgatc gggtcgcccg
      961 acagcggccc gatcttgccg ccgaggcgga gcgccagccg cgcgccgggg ccagcctcga
     1021 aggcgatctt ggccgcgacc ggatcccaga gcgggcccag gcaggcgttg ccgatgccgc
     1081 gttcgagcat gcggcgcagg atgaaggtcg agtcgccggg agcgccgccg cccgggttgt
     1141 cggcgccgtc ggaaaggacg accgggccgc cctcgaaggc gagcgcttcg tcgagcgctt
     1201 cgtcgatgcc aggaaagcgt atccgcaact gctcgcgcat gccgatcagc tcgtcggcga
     1261 gacgccgcgc catcgtcgcc gccttcgccg cgtcgtcgtc ggcgtagacg aggatctgcg
     1321 tgcccatgtg ctcggtgtcg ccccacgcga aaccgtgcga caccgagatc gacaggatgc
     1381 cgtccttgcc ttcgagcgac tggatccggt cgacgaaggc gcgcgccggc tggcgcgagg
     1441 tgtgcatcgt caccagcatt tcgcaatcga ccgcggcggc caccgggcgg agccgcttct
     1501 cgaccgtcgc cgcacacagg tcgacgagct cccaggcgcg ctcgaggacg tcggtatgcg
     1561 gatattcctt gaacgagatc agaagatcgg cgttcgtgag catcgccggc gtcaggtggt
     1621 tgtgcggatc gagctcggcg ccgacgacga ccttcggacc gacgatctcg cgaaccttcg
     1681 ccagcagatc gccctcgcag tcgtcgtagc catcggcgac catggcgccg tgcaggccga
     1741 gcacgaccat gtcgaccggc atggcggcgc gcaggtcgcc gagcagctcg tcgcgaagga
     1801 gctcgtaggc ggcacgcgtc gtcgttcctc cgggctgggc ggcggcgacc atgccttcga
     1861 ccagggtcca gccgcgctca cgcgcccgtt gtcgcgccgc ccagagcggc gcggcgaaga
     1921 agctgagcgc ctcgggatgc gtcccggcgg cgtagtagcc ccggtcgcga aaggcgccga
     1981 gcccggtcgg catcggcgcg aaggtgttgg tttcggtggc gaggcagccg gtgaagacgc
     2041 gcatgaggtg gggaaggagc agacgcgggg cggtcgatgg tatctcctgc gtcgtgcccg
     2101 gccgcgccag gccggcgaag cgagcgtcgc gacaatggcg ccatggtctt ctcgaacgcg
     2161 gtcgcgccgc tggcgatgat cgccgccacc gtgctcgccg gcgccttcgt gcaaggcacg
     2221 accggcgtcg gcttcgccct ggtcgtcgct cccgtcctcg gtctgctcgc gccggagttg
     2281 ctgcccgcca ccgtcttgct gctgatggtg ccgctcaacg tctacgtggt ctggcgcgaa
     2341 cgcggctcga tcgaccggct cggcgccgcc tggatcaccg gcggccgcct cgtcggcacc
     2401 gcaggcggct tctgggtcct cgtcgccctg agcgtcggcc ggttgagcct cttcgtcggc
     2461 ttggcgacgg cgctcgccgt cctcgcgaca ctggccgcgc cccgcttcac gccgagccgc
     2521 tcggccttcg tcgccgccgg cgtcgtcacg ggcatcaccg agaccgcgac gggcatcggc
     2581 gggccgccgc tcgccctcgt gtaccagcac catcccgtcg cgacgatgcg tccgacgctg
     2641 gcgctgtgct tcctgctcgg ccagctgatg tcgctggcga ccctggcgat cgccggacgc
     2701 atcggttcgg cgcagtggct ggcgacggcc gggctggtgc cggcgctcgc gctcggcgca
     2761 tggctcagcc accgcgtgca ccgcagcgtc ggcggccgct tccttcggcg cttcgtcctc
     2821 gccttcgcct tcgtgtcgtc gctcgcgctg atcgcgaagt cgctctgacg cgcggcgcga
     2881 gacgggccgt gcgccgaggg gcgcaggcag aacaatcgtc agtcgatgaa gagcagcgcc
     2941 gggtgctcga gccgttcgcg caccttct
//
LOCUS       DASRWS010000108         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_334434, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000108 DASRWS010000000
VERSION     DASRWS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334434"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFF72_01940"
     CDS             <1..579
                     /locus_tag="VFF72_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZV91945.1"
                     /translation="EGRPEPGAKMLVLNHVSWLDIMVVHAVVPEARFVAKADVKAWPL
                     VSQLVDSAGTLYLERERKRDALRVVHAIAGALRSGQSVAVFPEGTTSTGHGLLPFHAN
                     LLQAAIATATPVQPAALRFSDASHAVSEAMEFVGATTLRQSLWRSACGDRVVARLVFL
                     SPRDSAGAERRALAGRLRADIAGVLGLQPPEA"
     gene            complement(561..1307)
                     /gene="gpmA"
                     /locus_tag="VFF72_01945"
     CDS             complement(561..1307)
                     /gene="gpmA"
                     /locus_tag="VFF72_01945"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HZV91946.1"
                     /translation="MHKLVLVRHGESTWNLENRFTGWTDVELTDKGVEQARSAGRLLK
                     QERFEFDRAWTSVLKRAVWTLWHALDAMDRTWLPVSNDWRLNERHYGALQGLDKAETA
                     RKFGDAQVLAWRRSYDVPPPALPEGDPRSERDDPRYRHLAAGDVPRTECLKDTVARVL
                     PFWHGTLAPAIRAGERVIVSAHGNSMRALVKYLDHVSDSDIVGLNIPNGIPLVYELDD
                     ALAPIRSYYLGDAEAAAAAAAAVARQASGG"
     gene            1415..1825
                     /locus_tag="VFF72_01950"
     CDS             1415..1825
                     /locus_tag="VFF72_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZV91947.1"
                     /translation="MDFVLHNWYLFALALVSGGMLVWPMLSGVAGGERVTAADAVRLI
                     NRERAVLIDVSEPAEYAAAHPVGARSVPLASLETSRDLPKNKSTPVVVVCPSGSRAPR
                     AVATLKKLGFENARVLAGGLAAWRAANLPVEKTA"
     gene            1836..2099
                     /gene="grxC"
                     /locus_tag="VFF72_01955"
     CDS             1836..2099
                     /gene="grxC"
                     /locus_tag="VFF72_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZV91948.1"
                     /translation="MQAVRMYSTATCPFCLRAKALLRQRGVETIEEIRIDERPDERDA
                     MMRLTNRRTVPQIFIGETHVGGCDDLMALDRSGGLEPLLAGTR"
     gene            2174..2656
                     /gene="secB"
                     /locus_tag="VFF72_01960"
     CDS             2174..2656
                     /gene="secB"
                     /locus_tag="VFF72_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036230692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZV91949.1"
                     /translation="MADDTTSAPLFQIQRMYLKDLSLEQPNSPQVLLEQGTPQVEINL
                     ALGTETIGDGMYEVTVTATVTTKINDKTLFLVEAKQGGIFEIRNIPEEQLKQILGIAC
                     PGIVYPYLRAIVSDVCTRAGFPPVVLSEVNFQAMYEAQLAQAKAQTPTNGSGVIGTIN
                     "
     gene            2667..>3512
                     /locus_tag="VFF72_01965"
     CDS             2667..>3512
                     /locus_tag="VFF72_01965"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZV91950.1"
                     /translation="MNLTVLGAGAWGTALAVHAAAMRPTRLWVRDAAQAARMRSLRRN
                     ARYLADIALPPALQITTDFQAALRHGRDGVVVIATPMSGLEETLRRVEAEDAPMPLLW
                     LCKGFQEGTGWLGHEVARVAAPRLRCGVLSGPSFALEVARGQPTALVAASVEASVCEA
                     AVAALHGANLRVYTSSDPIGVEVGGAVKNVLAIATGLADGMRLGLNARAALVTRGLAE
                     MTRLGVALGADATTFMGLSGLGDLVLTTTGDLSRNRRVGLGLAEGVPLARIQADLGHV
                     AEGVSS"
BASE COUNT          503 a         1208 c         1234 g          567 t
ORIGIN      
        1 gagggcaggc ccgagcccgg cgcgaagatg ctcgtcctca atcatgtctc ctggctcgac
       61 atcatggtcg tccacgcggt cgttcccgag gcgcgcttcg tggccaaggc cgatgtcaag
      121 gcctggccac tggtgtcgca actcgtcgac tccgcgggaa ccctctacct cgagcgcgaa
      181 cgcaagcggg atgcgctccg ggtcgttcat gcgatcgccg gggcgctgcg gtcaggtcaa
      241 agcgtggcgg tcttcccgga gggcacgacg tcgaccggtc acggcctgct gccgttccac
      301 gccaatctgc ttcaggcggc gatcgcgaca gcgactccgg tgcaaccggc ggcgcttcgc
      361 ttttccgatg ccagccacgc cgtgagcgag gcgatggagt tcgtcggcgc gacgacgctc
      421 aggcaaagcc tctggcgttc ggcctgcggc gaccgtgtcg tcgcccggct cgtcttcctt
      481 tcgccaaggg acagcgcagg tgccgagcgg cgggcgctcg ccggcaggct gcgcgccgat
      541 atcgccggcg tgctcggcct tcagccgccc gaggcctgac gggcgaccgc ggcagcggcc
      601 gccgccgccg cttcggcatc gccgaggtag tagcttcgga tcggcgccag cgcgtcgtcg
      661 agctcgtaca ccagcgggat gccgttcggg atgttgaggc cgacgatgtc gctgtcggac
      721 acgtggtcga gatacttgac gagcgcgcgc atgctgttgc cgtgcgccga gacgatgacg
      781 cgttcgccgg cgcggatcgc cggcgccagc gtgccgtgcc agaaaggcag gacgcgggcg
      841 accgtgtcct tcaggcattc ggtgcgcggc acgtcgccgg cggcgaggtg gcggtagcgt
      901 ggatcgtcgc gctcgctgcg cgggtcgccc tcgggcagcg ccggtggcgg cacgtcgtag
      961 ctgcgccgcc acgcgaggac ctgggcgtcg ccgaacttgc gcgccgtctc ggccttgtcg
     1021 aggccttgca gggcaccgta gtgccgttcg ttgagccgcc agtcgttgct gaccggaagc
     1081 caggtccggt ccattgcgtc gagcgcgtgc cagagggtcc agacggcgcg cttcaggacc
     1141 gacgtccagg cgcggtcgaa ttcgaaacgc tcctgcttca gcaggcggcc tgcgctgcgg
     1201 gcctgctcga cgcccttgtc ggtcagctcg acgtcggtcc atccggtgaa gcgattttcc
     1261 aggttccagg tcgattcgcc gtggcgaacg aggacgagct tgtgcatcgg aatgggccgg
     1321 gccgggccgg aagcgatggc cttgcgaggc gctgccaaag cgcgaattct ataattcgcg
     1381 ttttcccgag ccggcggtcg cgccgacgac gaccttggat tttgtccttc acaactggta
     1441 cctcttcgcg ctcgccctcg tgtcgggagg catgctggtc tggccgatgc ttagcggcgt
     1501 cgccggaggc gagcgggtga cggcggccga cgcggtgcgc ctcatcaatc gcgagagagc
     1561 cgttctcatc gacgtcagcg agccggccga atatgcggcg gcgcacccgg tcggcgcccg
     1621 cagcgtgccg ctggcgagcc tcgaaacgtc gcgcgacctg ccgaagaaca agtcgacgcc
     1681 ggtggtggtc gtctgcccga gcgggtcgcg tgcgccacgt gccgtggcga cgctgaaaaa
     1741 gctcggcttc gagaatgccc gcgttctcgc cggcggcctt gcggcatggc gcgccgccaa
     1801 cctgccggtc gagaaaacgg cctgaccctg acgcgatgca ggcagttcgc atgtacagca
     1861 cggcgacgtg ccccttctgc cttcgtgcca aggcgctcct gcgccagcgc ggtgtcgaga
     1921 cgatcgagga gatccgaatc gacgaacgac ccgacgagcg cgacgccatg atgaggctga
     1981 cgaaccggcg cacggtgccg cagatcttca tcggcgaaac gcacgtcggt ggctgcgacg
     2041 acctgatggc gctcgatcgg tccggcggcc tcgagccgct tctcgccggc acgagataat
     2101 cgccgaccgg caaccggccg ggtccgttgc gccgccgctg ccgcggcgcg ttccctcctc
     2161 tcgagaattt cgcatggccg acgacaccac ttccgccccc ctgttccaga ttcagcgcat
     2221 gtacctgaag gatctttcgc tcgagcagcc gaattcgccc caggtgctgc tcgagcaagg
     2281 cacgccgcag gtcgagatca atctcgcgct gggtaccgag acgatcggcg acggcatgta
     2341 cgaagtgacc gtcaccgcga ccgtgacgac gaagatcaac gacaagacac tctttctggt
     2401 cgaggccaag cagggcggca tcttcgagat ccgcaacatc cccgaggagc agctgaagca
     2461 gatcctcggc atcgcctgtc cgggcatcgt ctatccctat ctgcgagcga tcgtctccga
     2521 cgtctgcacc cgcgccggct ttccgccggt ggtgctcagc gaggtcaatt tccaggccat
     2581 gtacgaggcg cagctggcgc aggcgaaggc gcagacgccc accaacgggt cgggcgtcat
     2641 cggcacgatc aactgacggc gcgagcgtga acctcacggt gctcggcgcg ggtgcctggg
     2701 gaacggccct cgccgtgcac gcggccgcca tgcggccgac gcgactttgg gttcgcgatg
     2761 cggcgcaggc ggcgaggatg cgctcgctgc gtcgcaacgc gcgctatctt gccgacatcg
     2821 cgctgccgcc ggcgctgcag atcacgaccg acttccaggc ggcgctgcgc cacggccgcg
     2881 acggcgtcgt cgtcatcgcg acgccgatgt cgggcctcga ggagacgctg cgccgggtcg
     2941 aggcggaaga cgcgccgatg ccgctgctgt ggctttgcaa gggattccag gagggcaccg
     3001 gctggctcgg ccacgaagtc gcccgcgtcg cggcgccgcg cctgcgttgc ggcgtcctgt
     3061 cggggccgag cttcgcgctc gaggtcgcgc gcggccagcc gaccgcgctg gtcgccgcca
     3121 gcgtcgaggc ctcggtctgc gaggcggctg ttgcggcctt gcatggcgcc aacttgcggg
     3181 tctacacatc ctccgatccg atcggcgtcg aggtcggcgg cgccgtcaag aacgtcctgg
     3241 ccatcgccac cggcctcgcc gacggcatgc ggctcggcct caacgcccgc gctgcgctgg
     3301 tcacgcgcgg cctggccgag atgacgcgtc tgggcgttgc gctcggcgcc gacgcgacga
     3361 cgttcatggg gctctcgggc ctcggcgacc tggtgctgac gaccaccggc gacctgtcgc
     3421 gcaatcgtcg cgtcggcctc gggctcgccg agggcgtgcc gctggcgcgc atccaggccg
     3481 atctcggtca cgtcgccgag ggcgtctcga gc
//
LOCUS       DASRWS010000109         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3490284, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000109 DASRWS010000000
VERSION     DASRWS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3490284"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFF72_01970"
     CDS             complement(<1..805)
                     /locus_tag="VFF72_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91951.1"
                     /translation="MAMDRLLMLDLAAGGCAVEAHLNGMPVASLAASGGSCSVAVHEY
                     TLAGRNHLSLVVGPAPPGVTVASQPKVAIGATWARARLVLVRHGQSPADPEARVLGVV
                     EWTTAEGKSYDAPSTHGRDVDLPVSFPRWRWLDAPPIAINTSVQRTVLEFMQNVAVEL
                     GRGNPDPLVAASKLRFDELALAYQTDANAGMQRFRDHLQRLYAAKALKVPPPAAADLV
                     LRPLVDGRLLECLAATGGPLLRTQNDAPDLGDQAWPVRIAMVEGKIYVLR"
     gene            complement(805..2577)
                     /locus_tag="VFF72_01975"
     CDS             complement(805..2577)
                     /locus_tag="VFF72_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91952.1"
                     /translation="MLRLSWEQFEQLEQLALRRRAAGIGAVLAEVWPALTERLQGRWS
                     AFVEAAVLQGRKHGLVDTADLARYAGLWCIWGPAFDSKPAFAWAAEILADPRRSSTLK
                     LHQLLHRTREELRRAELAVAKPGAAAPGASGAPALDSAQFDAALAKVDARVATLAASR
                     TVFPEATTTIAVKACDIGAIDLMIAEPDDLQEYRHTANGWHRTAVPRIESPALHWTHA
                     PEPAVELAVTSRPLRAGVPARMNLIVETVAACDVKVHPEIVHVAASGRLTWKGRDAAR
                     LSLALYALPRRPEDTLAGIGALTVADAQTVDVSSCGLRDAGAPFGAVKVGLRVYPASQ
                     WMLEIGHAAWSPMLWPAVPEAPVADARCRLECDGAVVDSASWQRAWSSLRGLFRQGLD
                     RLFNEWTRVLDGQATRLEVEASPLVGQAGLTWGWRRTSASTAGMRVEGALDLLAMSID
                     LRLSGEIVEGPARSRIRLLCKGRSELRTPISEIAGEGVEGQTLKSAVRNWRFPFSLEI
                     EPLAAAEPATLGVAGVAVPVQGALTGECGLRARADGAGHQWFFALRVEPISIVVELVD
                     PLLGARRQTRKICGAMTLVDWSAG"
     gene            complement(2597..3148)
                     /locus_tag="VFF72_01980"
     CDS             complement(2597..3148)
                     /locus_tag="VFF72_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HZV91953.1"
                     /translation="MSTAFSPAQLRASLWAREGGHVYAVIDGGVVPGIAERLAGADVA
                     GWDCLHRGALNAAAAASAPYLVQLKSVSTFTDWLLGEAADAFACWGVLAMSGQAILPM
                     REHCRTIGEVLTPDGQRRPWRWYDPEVLRTILPTLLPGQLDELFAGGQGLVVVEAAAW
                     TRFSIEQGVLAVDTRRVMAAASR"
BASE COUNT          531 a         1131 c         1074 g          455 t
ORIGIN      
        1 aacgcagtac atagatcttc ccctcgacca tggcgatgcg taccggccac gcctgatcac
       61 cgagatcggg cgcatcgttc tgggttcgca gcagcgggcc accggtggcc gccaggcatt
      121 cgagtaggcg gccatcgacg agtggacgca acacgagatc tgcagcggct ggcggcggaa
      181 ccttcaatgc tttcgcagca tacagccgct gcagatgatc tcgaaagcgc tgcatgccgg
      241 cgttcgcgtc ggtctggtag gccagggcca gctcgtcgaa gcgaagcttc gacgcggcca
      301 ccagcggatc gggattgcct cggccgagct cgacggcgac gttttgcatg aactcgagga
      361 cggtgcgctg caccgacgta ttgatcgcga tcggcggcgc atcgagccag cgccagcgcg
      421 gaaagctcac cggcaggtcg acgtcgcggc cgtgcgtcga cggtgcgtcg tacgacttgc
      481 cttcggcagt cgtccattcg acgacgccga gcacgcgcgc ctcggggtcc gccggcgact
      541 gcccatgccg gacgagaacg aggcgcgcgc gcgcccacgt ggctccgatc gccaccttcg
      601 gctggcttgc gaccgtgacg cccggcggcg ccgggccgac gacgagcgaa aggtggtttc
      661 ggcctgccag cgtgtactcg tgcacggcga cgctgcagct tccgccgctt gcggcaagcg
      721 acgccaccgg catgccgttg agatgtgcct cgacggcgca cccgcccgca gcgaggtcga
      781 gcatcagcag ccggtccatc gccatcagcc ggcgctccag tctacgagcg tcatcgcgcc
      841 gcagatcttg cgtgtctgtc gccttgcgcc gagcagtgga tcgacaagct cgacgacgat
      901 cgatatcggc tcgacacgga gagcgaagaa ccactgatgg ccggcgccgt cggcgcgagc
      961 gcgcagaccg cattccccgg tcagcgcccc ctgcaccggc acggcgacgc ccgcaacgcc
     1021 gagcgtcgcc ggctcggcgg cggcgagagg ctcgatctcg agactgaatg gaaaccgcca
     1081 attgcggacc gccgacttga gcgtctgtcc ctcgacacct tcgccggcga tttcgctgat
     1141 cggcgtgcga agctcgctgc gccctttgca gagaaggcgg atgcgcgagc gcgccggccc
     1201 ttcgacgatt tctcccgaga gacgaagatc gatcgacatc gccagcagat cgagcgctcc
     1261 ctcgacccgc atgccggcag tcgaagccga cgtccgccgc cacccccacg tcaggccggc
     1321 ctgcccgacc agcggggaag cctcgacttc gagccgcgtc gcctggccgt cgaggacacg
     1381 cgtccattcg ttgaaaagcc gatcgagacc ctggcggaaa aggcctctga ggctcgacca
     1441 tgcccgttgc cacgaggcgc tgtcgacgac cgctccgtcg cattccaggc gacagcgagc
     1501 gtctgcgaca ggtgcttcgg gaaccgcagg ccacagcatg ggtgaccacg ccgcatggcc
     1561 gatctcgagc atccactggc tcgccggata gacgcgcaat cccaccttga cggcgccgaa
     1621 gggcgcgccc gcgtcgcgca ggccgcaact cgagacgtcg acggtctgcg cgtcggcgac
     1681 ggtcaatgct ccgattcccg cgagcgtgtc ctccggcctg cgcggcagtg catagagcgc
     1741 caggctcagt cgcgcggcgt cgcgaccctt ccacgtcaga cgcccggatg cggccacgtg
     1801 cacgatttcc ggatggacct tcacgtcgca ggctgcgacc gtctcgacaa tgagattcat
     1861 ccgcgccggc acgccggcgc gcagcggtcg gcttgtgacc gcaagctcga ccgcaggctc
     1921 gggcgcgtgc gtccagtgca gcgccggcga ctcgattcgt ggcacggcgg tgcgatgcca
     1981 gccattggcg gtatggcgat attcctgaag atcgtcaggc tcggcgatca tgagatcgat
     2041 ggcgccgatg tcgcacgcct tcaccgcgat cgtcgtcgtc gcctcgggaa acacggtccg
     2101 ggacgcggcg agcgtggcta cacgcgcatc cactttcgcc agcgcggcgt cgaactgggc
     2161 gctgtccagc gccggcgcgc cggacgcacc gggcgccgcg gcccccggct tcgccacagc
     2221 gagctcggcg cggcgcaact cctcgcgcgt gcgatgcagt agctggtgaa gcttgagggt
     2281 cgagcttcgt cgcgggtcgg cgaggatctc ggccgcccac gcgaaggccg gcttgctgtc
     2341 gaaggccggc ccccagatgc accagagccc cgcatagcgc gccagatcgg cggtgtcgac
     2401 aaggccgtgc ttgcgaccct gaagcaccgc ggcttcgacg aaggccgacc agcggccctg
     2461 cagtcgctcg gtgagcgcgg gccagacctc ggccaggacg gcgccgatcc ccgcggcacg
     2521 gcgcctcagc gcgagctgct cgagctgctc gaactgctcc cacgacagcc tcagcatgga
     2581 cgttgcgccc gcggcttcat cgcgacgctg ccgccatcac ccgccgcgtg tcgaccgcca
     2641 ggacgccctg ctcgatcgag aaccgggtcc aggcggcggc ctcgacgacg accaggccct
     2701 ggccgccggc gaacagctcg tcgagctgac cgggcaagag cgtgggcagg atcgttcgca
     2761 gcacctcggg gtcgtaccag cgccatgggc gacgttgccc atcgggcgtc agaacctcgc
     2821 cgatcgtccg gcagtgctcg cgcatcggca gaattgcctg gccagacatg gcgagcaccc
     2881 cccagcaggc gaatgcgtcg gcggcctcgc cgagcaacca gtcggtgaag gtcgaaacgg
     2941 acttgagctg caccagatat ggcgcgctcg ccgcagccgc agcattcaat gcgccgcgat
     3001 gcaggcagtc ccacccggcg acgtccgcgc ccgcaagcct ttcggcaatg ccgggaacca
     3061 cgccaccgtc gatcacggcg tagacatggc cgccttcgcg cgcccagagc gaggcgcgca
     3121 gctgggcggg cgaaaacgcg gtgctcatcg agcgggaggt cgaagtcgat caggacgagg
     3181 aagagtcctt g
//
LOCUS       DASRWS010000110         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2634092, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000110 DASRWS010000000
VERSION     DASRWS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634092"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2479
                     /locus_tag="VFF72_01985"
     CDS             <1..2479
                     /locus_tag="VFF72_01985"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZV91954.1"
                     /translation="ASAAPFVADVDKLGLHLQADAVIGKGAPALTIDKVGVDVHGVRL
                     VADRATEPFLQATQIALEDGRADLAARRITLGRLAVSGGRVMVTREANGNVPLLDAFT
                     ARPVSHEDRVTKPAAEGVAAAPGTAWHYRVEHVETHGFDVALNDRSVDPPLQATLVGI
                     EADAKGLSDDPKAAVPVSLKFSVKEGGRFEAHGSVVPSAPSADLQLRIADLALRPGQP
                     LLARYANLELVRGKAATAGRLRYRDGRFRFEGSASIDDLLVDEVPNKERFIAWKDLST
                     KRLVVTPDRLSTDEVMLDRLDTKLVIFKDRTVNVARILKRDASAPTPAAKADDAAALA
                     TMSSPRAAGEAMVGAQTVPPLRRGAGSAHAAAAGPHASKPSAFRVSVGRIRVSRGDLD
                     YADLSLALPFAARIHGLKGNIVGLSSEPGRPTTIELGGQVNEYGLARAAGSIDLFDPA
                     AFTDIKVVFRNIEMTELTPYSATFAGRKIASGKLSLDLEYKVEDRQLAGDNQVVMDQL
                     TLGERVDSPGAVNLPLDLALALLQDSDGKIELGLPVSGSLDDPKFSYGSLIWKAVVNV
                     VTRIVTAPFRALGGLFGKSDGKSAKVTFDAGSAALLPPEREKLNQLAQSLGKRAALSL
                     TVHATYVAATDRDALQELRLRRDVAAAAGRTPGPDENPGPIVTSDPKTQAALERLFAQ
                     RFGADAVAQLKRQIGQANPAPPPKAASARILDDVSGLFKAKETPLSAQQASELKGADA
                     YQVMLRRLLASEKIGDDDLVALAKARGDAIRRQLTSHGVATERIATEAPGAGPGKEPE
                     ATLDLGVKGRGTPAGPSASSPSAAGRT"
     gene            complement(2476..>2752)
                     /locus_tag="VFF72_01990"
     CDS             complement(2476..>2752)
                     /locus_tag="VFF72_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZV91955.1"
                     /translation="MAGRALPGPVDYPQIPALAERGLARVRQFLVALDHLLDGHDYVA
                     ADRFSVADITAVVAVDCARVVKIRPDDGHPHLLRWRAAMAGRPAMSL"
BASE COUNT          451 a          978 c          945 g          378 t
ORIGIN      
        1 ggcaagcgcg gcgcccttcg tcgccgacgt cgacaagctc ggcctccacc tgcaggccga
       61 cgcggtcatc ggcaagggcg ccccggcact caccatcgac aaggtcggag tcgacgtgca
      121 cggcgttcgc ctcgtcgccg atcgcgcgac ggagccgttc ctccaggcga cgcagatcgc
      181 tctcgaagac ggccgcgccg acctggccgc gcgccgcatc acgctcggcc gcctcgccgt
      241 gtcgggcgga cgcgtgatgg tcacgcgcga ggcgaacggc aacgtgcccc tgctcgacgc
      301 gttcacggcc aggcccgtgt cgcatgagga ccgggtcacg aagccggcgg ccgagggcgt
      361 cgcagcggct cccggtactg cgtggcatta ccgcgtcgag catgtcgaaa cgcacggctt
      421 cgacgtggcg ctgaacgatc gctcggtcga tccgcctctg caggcgacgc tggtcggcat
      481 cgaagccgac gccaaggggc tcagcgacga tccgaaggca gccgtgccgg tgagcctgaa
      541 gttctccgtc aaggaaggcg gacgattcga ggcgcacggc agcgtcgtgc cgagcgcgcc
      601 ttcggccgat ctgcagctgc gcatcgccga cctggcgctt cgccccggcc agccgctgct
      661 cgcgcgatac gccaatctcg agctcgtgcg cggcaaggcc gcgaccgccg gcaggctgcg
      721 ctaccgtgac ggcaggttcc gcttcgaagg cagcgcgtcg atcgacgacc tgctcgtcga
      781 cgaagtgccg aacaaggagc gcttcatcgc ctggaaggat ctctcgacga agcgactcgt
      841 cgtgacgccc gatcgcctct cgaccgacga ggtgatgctc gaccggctcg acacgaagct
      901 cgtcatcttc aaggatcgca ccgtcaacgt cgccaggatc ctcaagcgag atgcttcggc
      961 cccgacgccg gcggcaaaag cggatgacgc cgcggccctg gcgacgatgt cgtcgcctcg
     1021 cgctgcaggg gaggcgatgg tcggggcgca gaccgtaccg ccacttcgcc ggggcgcagg
     1081 gtcggcgcac gccgccgcgg cgggaccgca cgcatcgaag ccgtcggcct ttcgcgtcag
     1141 cgtcggacgg atacgggtaa gccgcggcga tctcgactac gccgacctgt cgctcgcgtt
     1201 gccgttcgcc gccaggatcc acggcctgaa gggaaacatc gtcggcctgt cgagcgagcc
     1261 gggccggccg acgacgatcg agctcggcgg ccaggtcaac gagtacggtc tggcgcgcgc
     1321 cgccggctcg atcgacctgt tcgatcccgc cgccttcacc gacatcaagg tcgtcttccg
     1381 caacatcgag atgaccgagc tgacgcccta ctcggccacc ttcgccggcc gcaagatcgc
     1441 ctcgggcaag ctctcgctcg atctcgaata caaggtcgag gaccgccagc tggctggcga
     1501 caaccaggtc gtgatggacc agctgacgct gggcgaacgc gtcgacagcc cgggcgccgt
     1561 gaacctgccg ctcgatctcg cgctcgccct gctgcaggac agcgacggca agatcgagct
     1621 cggcctgccg gtctcgggca gcctcgacga tccgaagttc agctacggca gcctcatctg
     1681 gaaggcggtc gtcaacgtcg tcaccaggat cgtcacggcg ccgtttcgcg ccctcggcgg
     1741 actcttcggc aagagcgacg gcaagtcggc caaggtgacg ttcgacgccg gcagcgcggc
     1801 gctccttccg cccgagcgcg agaagctgaa ccagctcgcg cagtcgctcg gcaagcgcgc
     1861 cgcgctgtcg ctcaccgtgc atgcgaccta cgtcgcggca accgatcgcg atgcgctgca
     1921 ggaactgcgc ctgcgtcgcg acgtggccgc ggcggccggg cgcacgccgg ggcccgacga
     1981 gaatccgggg ccgatcgtca cgagcgatcc gaagacgcag gcggcgctgg aaagactctt
     2041 cgcgcagcgt ttcggcgccg atgcggtggc gcagttgaaa cgccagatcg gccaggccaa
     2101 tccggcgcca ccgccgaagg cggcttcggc tcggatcctc gacgatgtct caggtctctt
     2161 caaggcgaag gagacgccac tgtcggcgca gcaggccagc gagctgaagg gtgccgatgc
     2221 ctaccaggtc atgctccggc gcctcctcgc cagcgagaag atcggcgacg atgatctcgt
     2281 ggccctcgca aaggcacgag gcgatgcgat ccggcgccag ttgacgtctc acggcgtcgc
     2341 cacggaacgc atcgcgaccg aggcgccagg ggcaggcccg ggcaaggaac ccgaggcgac
     2401 gctcgacctc ggcgtcaaag gccgcgggac gcccgccggg ccttcggctt cctcgccttc
     2461 ggcagcgggc cggacctaaa gcgacatcgc cggccggccg gccatcgccg cacgccagcg
     2521 cagcagatgc ggatgcccgt cgtccggcct gatcttgacg acgcgcgcgc aatcgacggc
     2581 gacgaccgcg gtgatgtcgg cgacgctgaa gcgatcggcg gccacgtagt cgtgaccgtc
     2641 gagaaggtgg tccagggcga cgaggaattg ccgtacgcgc gccagcccgc gttctgccag
     2701 cgcggggatc tgcgggtagt cgaccgggcc gggcagcgcc cttcccgcca tc
//
LOCUS       DASRWS010000111         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_622163, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000111 DASRWS010000000
VERSION     DASRWS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622163"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2457
                     /gene="secA"
                     /locus_tag="VFF72_01995"
     CDS             <1..>2457
                     /gene="secA"
                     /locus_tag="VFF72_01995"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZV91956.1"
                     /translation="EAGKRTLGQRHFDVQLIGGMILHEGSISEMKTGEGKTLVATLPV
                     YLNALTGRGVHVVTVNDYLAKRDAEWMGQIYGFLGLTTGVIVHGIDDEERKKAYDCDI
                     TYGTNNELGFDYLRDNMKYRMEDMVQRGHVYAIVDEVDSILVDEARTPLIISGPLDDR
                     SEFYNTIDTYIPQLDKSDYEVDEKQRTVTMTEAGMEKMETVLREAGLLKGGKLYDVEN
                     VSVVHHVNQALRAHKLFQRDKDYIVRNGEVVIIDEFTGRMMPGRRYSEGLHQALEAKE
                     HQPIQPENQTLASITFQNYFRMYEKLAGMTGTALTEAEEFGDIYNLDVLEVPTNKPLA
                     RIDDDDEVYRTAAEKNRSIITLIDDCRKRGQPVLVGTTSIEKSEHLAELLRKDGWEQH
                     DFSDPNAFRDLYSGETQKKVFAILNARYHEQEAFIVSQAGVPGAVTIATNMAGRGTDI
                     QLGGNADMRIRQELTDISDWGERDRHPRAAEIREQVAKLKDKALAAGGLFVLGTERHE
                     SRRIDNQLRGRSGRQGDPGHSKFFLSLEDDLMRIFGSDKLDGMLQKLGLKEGEAIVHP
                     WINKALEKAQQKVEARNFDIRKNLLKYDDVMNDQRKVIFEQRLDLMRDEAVDETIADM
                     RHEVVDELVAKHIPPNAYAEQWDAAGLHEALLNVLTLDLPVQEWAKEEGIADEEMKER
                     IERRADEFMAAKVAKWGPDVMRYVEKSILLQTLDHLWREHLVMLEHLRQVIGLRGYGQ
                     RDPLNEYKAEAFSLFEAMMSRLREAVTSQLMRVEIVQQQPEEPPPQQLPYMEAHKVDP
                     TTGEDELAPALAPMLAGAGNG"
BASE COUNT          529 a          784 c          786 g          358 t
ORIGIN      
        1 gaggcgggca aacgcaccct tggtcagcgc catttcgacg tgcagctcat cggcggcatg
       61 atcctgcacg agggcagtat ctccgaaatg aagaccggcg aaggcaagac gctggtcgca
      121 accttgcccg tctatctcaa cgcgctcacc ggccgtggcg tgcacgtcgt gacggtcaac
      181 gattacctcg ccaagcgcga cgctgaatgg atgggccaga tctacggctt cctcgggctg
      241 acgacaggcg tcatcgtgca cggcatcgac gacgaggagc gcaagaaggc ctacgactgc
      301 gacatcacct acgggaccaa caacgagctc ggcttcgact atcttcgcga caacatgaag
      361 taccggatgg aagacatggt ccagcgcggc cacgtctatg ccatcgtcga cgaagtcgac
      421 tcgatcctgg tcgacgaggc gcggacgccg ctgatcatct ccggcccgct cgacgaccgc
      481 tccgagttct acaacaccat cgacacgtac atcccgcagc tcgacaagag cgactacgag
      541 gtcgacgaga agcagcgcac cgtcaccatg accgaagccg gcatggagaa gatggagacg
      601 gtgctgcgcg aggcggggct gctcaagggc ggcaagctct acgacgtcga gaacgtctcg
      661 gtcgtgcacc acgtcaacca ggcgctgcgc gcgcacaagc tgttccagcg cgacaaggat
      721 tacatcgtga ggaacggcga ggtcgtcatc atcgacgagt tcactggccg catgatgccg
      781 ggccgccggt attccgaagg gctgcaccag gcgctcgagg ccaaagagca ccagccgatc
      841 cagccggaga accagacgct cgcctcgatc accttccaga actacttccg catgtacgag
      901 aagctcgccg gcatgacggg cacggcgctc acggaggccg aagagttcgg cgacatttac
      961 aatctcgatg tgctcgaggt gccgaccaac aagccgctcg cgcgtatcga cgacgacgac
     1021 gaggtctatc gcaccgcggc ggagaagaac cgctccatca tcacgctgat cgacgactgc
     1081 cgcaaacgcg gccagccggt gctggtcggt acgacgtcga ttgaaaagtc ggagcatctc
     1141 gcggagctgc tgcgcaagga tggctgggag cagcacgact tctccgaccc gaacgcgttc
     1201 cgcgatctct attccggcga aacgcagaag aaggtcttcg ccatcctgaa cgcccgctat
     1261 cacgagcagg aagcgttcat cgtcagccag gcgggcgtgc ccggggccgt caccatcgcg
     1321 accaacatgg ccggccgcgg cacggacatc cagctcggcg gcaacgccga catgcgcatc
     1381 cgccaggagc ttaccgacat ttccgactgg ggcgagcgcg accggcatcc gcgcgcggcc
     1441 gagatccgcg agcaggtagc caagctgaaa gacaaggcgc tcgcggccgg cggcctgttc
     1501 gtgctcggca cagagcgcca cgaaagccgc cgcatcgaca atcagctgcg cggccgctcc
     1561 ggccgtcagg gcgatccggg acactccaag ttcttcctgt cgctcgaaga cgacctgatg
     1621 cgcatcttcg gctccgacaa gctcgacggc atgttgcaga agctcgggct caaggaaggc
     1681 gaagccatcg tccatccctg gatcaacaag gcgctcgaga aggcgcagca gaaggtcgag
     1741 gcgcgcaact tcgacatccg aaagaacctg ctcaagtacg acgacgtgat gaacgaccag
     1801 cgcaaggtga tcttcgagca gcgactcgat ctcatgcgcg acgaagcggt cgacgagacg
     1861 atcgctgaca tgcgccacga agtggtcgat gaactggtgg cgaagcacat cccgccgaac
     1921 gcctacgcgg agcagtggga cgcggccggc ctgcacgagg cgctattgaa cgttctgacg
     1981 ctcgacctgc cggtgcagga atgggccaag gaagagggca tcgccgacga ggaaatgaag
     2041 gagcgcatcg agcgccgcgc cgacgagttc atggcggcga aggtggccaa gtggggcccc
     2101 gacgtcatgc gttacgtcga gaagtcgatc ctgttgcaga cgctggacca tctctggcgc
     2161 gagcatctcg tcatgctcga gcatctgcgc caggtgatcg gcctgcgcgg ctacggccag
     2221 cgcgatccgc tcaacgaata caaggcggag gccttcagcc tgttcgaggc gatgatgtcg
     2281 cgcctgcgcg aggcggtcac cagccagttg atgcgcgtcg agatcgtgca gcagcagcct
     2341 gaggagccgc cgccgcagca gcttccgtat atggaggcgc acaaggtcga cccgacgacc
     2401 ggcgaggacg agttggctcc ggcgctggcg ccgatgctgg ccggagccgg caacggc
//
LOCUS       DASRWS010000112         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3491486, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000112 DASRWS010000000
VERSION     DASRWS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3491486"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFF72_02000"
     CDS             complement(<1..722)
                     /locus_tag="VFF72_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91957.1"
                     /translation="MLALKLALVGASIVLATLAARRFGHSVSGTLSGIPMVAGPIMGF
                     VLIEQPVAHVRAIALATLVCLPAMVSHMVCFARAARHMRWYGALLVANAVFVAAGWLL
                     WQIALPLVVVCAVALLSPLAGMAVLPLRRADVPDRTVAVFIPRSELALRVAAAIALAA
                     VIMQGAALLPTMVSGLLLALPINGNVLPCFTLPRHGVAATEALLRGFLWGVFGFASFF
                     VVLVAALPTCGAALAYALAWLAA"
     gene            complement(785..4561)
                     /locus_tag="VFF72_02005"
     CDS             complement(785..4561)
                     /locus_tag="VFF72_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZV91958.1"
                     /translation="MTTSPLMATTRWQFWVDRGGTFTDVVGRRPDGSLATLKLLSENP
                     EQYRDAAVAGIRRLLGLAADEPITPDRVESVKMGTTVATNALLERKGEPTLLVVTRGF
                     RDALRIAYQDRPRLFEPHIVLPELLYARVVEADERVGAHGEIVRPLDEAGLRAALEAA
                     HADGLRSVAIVFMHGWRFTGHEAAAERLARAAGFTQVSVSHRVSPLMKLVGRGDTTVV
                     DAYLSPILRRYVDEVAAEMPGVRLYFMQSSGGLAEAGGFQGKDAILSGPAGGIVGMVR
                     TAERGLRAIDANTASRAGTSKEVAAEVPGLRVIGFDMGGTSTDVSHYAGEFERAFETR
                     VAGVRVRAPMMAIHTVAAGGGSVLGFDGARLRVGPHSAGANPGPASYRRGGPLTVTDA
                     NVALGKIQPDFFPRVFGPRGDEPLDAGTVTTQFATLADEIARATGVRRLPEEVAEGFI
                     DIAVGGMAGAIKKISVARGHDVTRYTLQCFGGAGGQHACRVADALGMSRVFIHPLAGV
                     LSAYGMGLADQAALRQAAVELPLDEAASAVASRLDELAAEAAAELVRQGVEAGRIAVD
                     RRVHIRYEGTDSALVVPFGDAPALREAFETAYRQRFAFLMRERRLVVEAVSVEAIGTG
                     ELAAESAWPLAASGAAPARALVRFFSEGGWHDVQLVERDDARPGHTIDGPAIVVERNA
                     TTIVEPGWRASVTPLDHLLLERIAPRAARRAIGTEVDPVLLEVFNNLFMNIAEQMGLQ
                     LQNTAYSVNIKERLDFSCALFDADGNLIANAPHMPVHLGSMSESIKTVLARNAGTMRP
                     GDVYVLNDPYHGGTHLPDVTVVTPVYLEERSPLPEGRRPSFPGAEDGGEGPHDAARAP
                     QAPAFFVGSRGHHADIGGTTPGSMPPFSTRIDEEGVQIDNVKLCENGRFLEDAMRALL
                     GSGAWPARNPDQNLADLKAQVAANEKGVRELRAMVADYGLDVVQGYMRHVQDNAEESV
                     RRVITRLSDGAFVLPLDNGAEIRVAIRVDAASRSAEIDFSGTSAQLSNNFNAPTAVCM
                     AAVLYVFRTLVDDEIPLNAGCLKPLRVVIPEASMLNPHPPASVVAGNVETSMCITNAL
                     YGALGTMASSQCTMNNFTFGNERYQYYETISGGSGAGPGFAGTSVVQTHMTNSRLTDP
                     EVLEFRYPVRLDSYEIRAGSGGGGAFRGGDGGIRRVRFLEPMTASILSNGRRHGAFGM
                     AGGEAGAVGVNRVERSDGRIETLGHIGSVEMRPGDVFVVETPGGGGYGAVARSL"
     gene            complement(4558..>5173)
                     /locus_tag="VFF72_02010"
     CDS             complement(4558..>5173)
                     /locus_tag="VFF72_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZV91959.1"
                     /translation="GTFPFPVAYRKVVGAAPRRVVVDADPRLLAPFVAAARDLAAEGV
                     RAIATSCGFLAIFQRELQAAVDIPVWTSSLLLVGGLERGLAEGRRVGIVTVDAASLSE
                     AHLRAAGAPVDAPIEGLAIDSALRRCLLDDLDHLDEDEAQRATVAAATRLVARHPEVA
                     VIVLECTNLPPYADAVRAATGRPVHDITTLIADRLPASTSANPT"
BASE COUNT          799 a         1824 c         1878 g          672 t
ORIGIN      
        1 gccgcgagcc aggccagcgc gtaggcgagc gccgcgccgc atgtcggcaa cgcggcgacg
       61 agcacgacga agaacgaggc gaagccgaac acgccccaca ggaagccgcg cagcagcgcc
      121 tcggtcgccg cgacaccatg gcgcggcagc gtgaagcagg gcagcacgtt gccgttgatt
      181 ggcagggcca gcagcaggcc gctgaccatg gtcggcagca gcgcggcacc ttgcatgatc
      241 accgccgcca gggcgatcgc ggccgccacg cgcaaggcca gctcgctgcg cggaatgaag
      301 acggcgacgg tacgatccgg cacgtcggca cggcgcaagg gaagcactgc catgccggcc
      361 agcggcgaca gcagcgccac cgcgcagacc acgacgagcg gcagcgcgat ctgccacagc
      421 agccagccgg cggcgacgaa caccgcgttg gccacgagca gcgcgccata ccaccgcatg
      481 tgccgcgccg cgcgggcgaa gcacaccatg tgagacacca tggccggcag gcacaccagc
      541 gtcgccaacg cgatcgcccg gacatgcgca accggctgct cgatcagcac gaaccccatg
      601 atcgggccgg cgaccatcgg aatgccgctc agggtgccgg agacgctgtg gccgaagcgc
      661 cgcgccgcca gcgtcgccag cacgatggac gcgccgacca gcgcgagctt gagcgcgagc
      721 acgcctcagg gcccggaatc gaatgggcgg gtcggcgacg ggccgcgccc cgccgggggc
      781 accgtcacag cgatcgcgcc acggcgccgt agcctccgcc gcccggcgtc tcgacgacga
      841 acacgtcgcc cgggcgcatc tccaccgagc cgatgtgccc gagcgtctcg atgcgtccgt
      901 cgctgcgttc gacccggttg acgcccaccg cgcccgcctc gccgccggcc atgccgaagg
      961 cgccgtggcg gcgcccgttc gacaggatcg acgcggtcat cggctcgagg aagcggacgc
     1021 gccggatgcc gccgtcgccg ccgcgaaacg cgccgccgcc acccgagccg gcgcggatct
     1081 cgtagctgtc gagacgcacc ggatagcgga actcgagcac ctccggatcg gtgaggcgcg
     1141 aattggtcat gtgcgtctgc accaccgacg tgccggcgaa gcccggcccg gcgcccgagc
     1201 cgccggagat cgtctcgtag tactggtagc gctcgttgcc gaaggtgaag ttgttcatcg
     1261 tgcactgcga cgacgccatc gtgccgagcg cgccgtagag cgcgttggtg atgcacatcg
     1321 acgtctcgac gttgccggcg acaaccgacg cgggcggatg cgggttgagc atcgaggcct
     1381 cgggaatgac gacgcgcagc ggcttcaggc agccggcgtt gagcggaatc tcgtcgtcca
     1441 ccagagtgcg aaagacgtag agcaccgccg ccatgcagac cgccgtcggc gcgttgaagt
     1501 tgttcgacag ctgcgccgag gtgccgctga aatcgatctc ggcgctgcgc gaggccgcat
     1561 cgacgcggat cgcgacgcgg atctcggcgc cgttgtcgag cggcagcacg aaggcgccgt
     1621 cggacaggcg ggtgatgacg cggcgcaccg actcctcggc gttgtcctgc acgtggcgca
     1681 tgtagccctg caccacgtcc aggccgtagt cggcgaccat ggcgcgcaac tcgcgcacgc
     1741 ccttctcgtt ggccgccacc tgcgccttca ggtcggcgag gttctgatcc gggttgcgcg
     1801 ccggccaggc gccgctgccg agcagggccc gcatcgcgtc ttcgaggaag cggccgttct
     1861 cgcacagctt gacgttgtcg atctgcacgc cctcctcgtc gatacgggtc gagaaaggcg
     1921 gcatcgagcc gggcgtcgtg ccgccgatgt cggcgtggtg gccacgtgaa ccgacgaaga
     1981 aggcgggcgc ctgcggcgcc cgcgcagcgt catggggccc ttctccgcca tcctccgccc
     2041 cgggaaagga gggccttctt ccttcgggca agggggaccg ttcctcgaga tacaccgggg
     2101 tcacgacggt gacgtcgggc aggtgcgtgc cgccgtggta gggatcgttg aggacgtaga
     2161 cgtcgcccgg ccgcatcgtc cccgcattgc gcgcgagcac ggtcttgatc gattcgctca
     2221 tcgagcccag gtgcaccggc atgtgcggcg cgttggcgat caggttgcca tcggcgtcga
     2281 agagcgcgca cgagaaatcg agccgctcct tgatgttgac cgagtacgcc gtgttctgca
     2341 gttgcagccc catctgctcg gcgatgttca tgaacaggtt gttgaagacc tcgagcagca
     2401 ccgggtcgac ctcggtgccg atggcccgcc tcgcggcgcg cggcgcgatg cgctcgagga
     2461 gcaggtggtc gagcggcgtg acgctcgccc gccagcccgg ctcgacgatg gtggtggcgt
     2521 ttcgctcgac gacgatcgcc ggtccgtcga tggtgtgccc gggccgcgcg tcgtcgcgct
     2581 cgacgagctg cacgtcgtgc cagccgccct cgctgaagaa gcgcaccagc gccctggccg
     2641 gtgccgcgcc gctggccgcg agcggccacg ccgactcggc cgcgagctcg ccagtgccga
     2701 tcgcctcgac cgacacggcc tcgacgacga ggcggcgctc gcgcatcagg aaggcgaagc
     2761 gctgccggta cgccgtctcg aacgcctcgc gcaaggccgg ggcgtcgccg aacgggacga
     2821 cgagcgccga atcggtgcct tcgtagcgga tgtggacgcg gcgatcgacg gcgatgcgcc
     2881 cggcctcgac cccctggcga acgagctcgg cggcggcctc ggcagcgagc tcgtcgagcc
     2941 gcgaggccac cgccgatgcc gcctcgtcga gcggcaactc gaccgccgcc tggcgcagcg
     3001 ccgcctggtc ggccaggccc atgccgtacg ccgagagcac gccggccagc ggatggatga
     3061 agacgcgcga catgccgagc gcgtcggcga cccggcaggc gtgctggccg ccggcgccgc
     3121 cgaagcactg cagggtgtag cgagtgacgt cgtggccgcg tgccaccgag atcttcttga
     3181 tcgccccggc catgccgccg acggcgatgt cgatgaagcc ttcggcaact tcctcgggca
     3241 accggcgcac gccggtagcg cgcgcgattt cgtcggcgag cgtggcgaac tgcgtcgtga
     3301 cggtgcccgc gtcgagcggc tcgtcgccgc gcggaccgaa cactcgcgga aagaagtcgg
     3361 gctggatctt gccgagggcg acgttggcgt cggtcaccgt cagcggcccg ccgcgccgat
     3421 acgacgccgg gcccgggttg gcgccggcgc tgtgcgggcc gacgcgcagc cgcgcgccgt
     3481 cgaagccgag aaccgagccg ccgcccgcgg cgacggtgtg gatcgccatc atcggcgcgc
     3541 gcacccgcac gccggcgaca cgcgtctcga aggcccgctc gaactcgccg gcgtagtggc
     3601 tgacgtcggt cgacgtgccg cccatgtcga aaccgatcac gcgcaggcca ggcacctccg
     3661 cggccacctc cttcgaggtt ccagcccggg atgcggtgtt cgcgtcgatg gcgcggagcc
     3721 cgcgctcggc ggtacgcacc atgccgacga tgccgccggc cgggcccgag aggatcgcat
     3781 ccttgccctg gaaaccgcca gcctcggcga ggccgccgga cgactgcatg aagtagaggc
     3841 gcacgcccgg catctcggcg gcgacctcgt cgacgtagcg gcgcaggatc ggcgagaggt
     3901 aggcatcgac cacggtggtg tcgccgcggc cgacgagctt catcagcggg ctgacgcgat
     3961 gcgagacgct cacctgcgtg aagccggcgg cccgcgccag gcgctcggcg gcggcttcgt
     4021 gcccggtgaa gcgccagccg tgcatgaaga cgatggcgac gctgcgcagc ccgtcggcgt
     4081 gggcggcctc cagcgcggcg cgcaagccgg cttcgtcgag cgggcgaaca atctcgccgt
     4141 gcgcgccgac gcgctcgtcg gcctcgacga cgcgggcgta gagcagctcg ggcaggacga
     4201 tgtgcggctc gaagagacgc ggccgatcct ggtaggcaat gcgcagcgca tcgcgaaatc
     4261 cgcgcgtgac gacgagcagg gtcggctctc cctttctctc gagcagcgca ttggtcgcga
     4321 ccgtcgtgcc catcttcacg ctttcgaccc gatccggcgt gatcggctcg tcggcggcga
     4381 ggcccagcaa gcggcggatg ccggcgacgg cggcatcccg atactgctcc gggttctcgg
     4441 aaagaagctt cagcgtcgcc agcgagccgt cgggccgccg gccgacgacg tcggtgaagg
     4501 tgccgccgcg atcgacccag aactgccagc gcgtcgtcgc catcaggggg ctggttgtca
     4561 tgtcggattt gcggaggtcg atgccggcag gcggtccgcg atcagtgtgg tgatgtcgtg
     4621 caccggacgt ccggtggcag cgcggacggc gtcggcgtac ggcggcagat tggtgcactc
     4681 gagaacgatc acggccacct cgggatggcg cgcgacgagg cgcgtcgcgg cggccaccgt
     4741 ggcgcgttgg gcctcgtcct cgtcgagatg gtcgaggtcg tcgagcaggc accttcgcaa
     4801 tgcggagtcg atcgccaggc cttcgatcgg cgcatcgacc ggggcgccgg cggcgcgcag
     4861 gtgcgcctcg gaaagcgacg ccgcatcgac cgtgacgatg ccgacgcgcc ggccttcggc
     4921 aaggccgcgc tcgagcccgc cgacgagcag caggctcgac gtccacacgg gaatgtcgac
     4981 ggcggcctgc agctcgcgct ggaagatcgc gaggaagccg cagctcgtcg cgatcgcccg
     5041 cacgccttct gcagccaggt cgcgcgccgc ggcaacgaac ggggcgagca gtcgcgggtc
     5101 ggcgtcgacg acgacccgcc tgggcgcggc gccgacgact ttgcgatacg ccaccgggaa
     5161 cgggaacgtg ccg
//
LOCUS       DASRWS010000113         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2634995, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000113 DASRWS010000000
VERSION     DASRWS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634995"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFF72_02015"
     CDS             <1..183
                     /locus_tag="VFF72_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91960.1"
                     /translation="PQFGTHNEPNPTASPEHGALPTVNVLTVSLAGSRRDTVFLGWFE
                     THTSLSTDIQSGAPGT"
     gene            394..>2402
                     /locus_tag="VFF72_02020"
     CDS             394..>2402
                     /locus_tag="VFF72_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZV91961.1"
                     /translation="METIRRPRKSLALAFSALVAVTVVRIAAQTPTSPAKATQPSGTP
                     TTAQVEFFEASIRPVLLETCGECHLDDEKGELRVDSRAALLKGGENGPAIVPGDPDKS
                     LLIQAIRRVGDAPKMPKKQAKLPDATIANFVEWVKQGAPWPTTTAASPSTPPAAKPPM
                     VITAEQRAWWSFRSLTKPLAPDVKDIEWPKTDVDRFVLARLEREGLQPVRPADRRTLI
                     RRATFDLTGLPPTMEEVEAFEKDTAPDAFAKVVDRLLASPRYGEAWARHWLDVARYAE
                     DDPRSLDPMNRGYNPYPNAYLYRDWVVRAFNDDLPYDRFVKAQLAADLFDEKDRVRAL
                     PALGFLGVGPWYYDNGSVEVTHADERHDRVDVVSRGFLGLTVACARCHDHKYDPIPQR
                     DYYGLAGVFLNTEYHEYPLAPKSVVDDYKALEKKKKNKQELLDDFTNTETRQLSETLA
                     LQSSKYMMAAWRVTGERKDDAAAAASSEKLDYELLQRWIRFLAKPPKFYPYLKDWQAM
                     IKDGGSEDEAKSLASTFQGLVTDVMFEAREIKDENDIIRAKALPGTKKKEKANLPNEF
                     VTNDDFCPGCGLELKSLSGDRVFLYADMFRGDLVDSADPANLKEDDKPGLFVFKGPAL
                     DRWLGADRRRYVEDLREDIESLGKAMPPKYSYVHGVADGAKAQTM"
BASE COUNT          455 a          780 c          779 g          388 t
ORIGIN      
        1 ccgcagttcg gcacccacaa cgagccgaat cccactgcga gccccgagca tggcgccttg
       61 ccaacggtga acgtgctcac ggtgtcgctc gcaggatcga ggcgggacac agtgttcttg
      121 ggctggttcg aaacccatac ctctttgtcg acggacatcc agtctggcgc tcccggcacc
      181 tgatagatcg ccgccggttt tagtttggtg atcgggatgc ggacgccggg cgtcgagacg
      241 ccgggacgcc gagccgctgc ggcgggggaa ggcgcgggcg cctgggcact tccgagcagc
      301 gatgcgcacg cgcacgcaaa gcatgtcgtc aatctacgca tcgggtctct tgggcactgc
      361 ggcataatat ccgcttcact gctgacgcga cgcatggaaa ccatccgacg gcctcgtaag
      421 tcactcgcgc tggcgttctc agcactcgtc gccgtcaccg tcgtccggat tgccgcgcag
      481 acgcccacgt cgccggcgaa agcgacgcag ccgtctggta cgcccaccac ggcgcaagtc
      541 gagttcttcg aagccagcat ccgcccggta ctcctcgaaa cgtgcggcga gtgccatctc
      601 gacgacgaaa agggggagct acgggtcgat tcccgtgccg cgctcctcaa gggcggcgag
      661 aacgggccgg cgatcgtgcc gggcgatccc gacaagagtc tcctcatcca ggcaatccga
      721 cgggtcggcg acgcgccgaa gatgccaaag aagcaggcga agctgcccga tgccacgatc
      781 gccaacttcg tcgaatgggt gaagcagggc gcgccctggc cgacgaccac cgcagcctcg
      841 ccgtcgactc cgccagcggc caagccgccg atggtgatca cggcggagca gcgggcgtgg
      901 tggtcgttcc gctcgctcac gaagccgttg gcccccgacg tcaaggacat tgagtggccg
      961 aagacggatg tggatcgatt cgtcctggcg cggctcgaac gcgaggggct ccagcccgtc
     1021 cgtccggcgg acaggcgcac gctgatccgc cgcgccacgt tcgatctcac tggcctcccg
     1081 ccgacgatgg aggaggtgga ggcgttcgag aaggacacgg cgcccgacgc gttcgcgaag
     1141 gtcgtcgatc gcctgcttgc gtcgccgcgg tacggtgaag catgggcacg ccactggctt
     1201 gacgtcgctc gctacgccga agacgatccg cgcagtctcg acccgatgaa ccgcggctac
     1261 aacccctacc cgaacgcgta cctgtatcgc gactgggtcg tccgcgcctt caacgacgac
     1321 ttgccgtacg accgcttcgt gaaggcgcag ctggcggcgg atctgttcga cgagaaggat
     1381 cgcgtccgcg cgttgccggc gctcggcttc ctcggcgtcg gtccctggta ctacgacaat
     1441 ggatcggtcg aggtgacgca cgccgacgag cgtcacgatc gtgtcgacgt cgtgtcgcgc
     1501 gggttcctcg ggctgacggt cgcctgcgcg cgctgccacg atcacaagta cgacccaatt
     1561 ccgcagcgcg actactacgg gctggccggc gtcttcctga acaccgagta ccacgagtat
     1621 ccgctcgcgc cgaaatccgt cgtcgacgac tacaaggcac tcgagaaaaa gaagaagaac
     1681 aagcaggagc tgctcgacga tttcaccaat accgaaacgc gccagctctc ggagacgctg
     1741 gcgctgcagt catcgaagta catgatggcc gcgtggcgtg tgacgggcga gcggaaagac
     1801 gatgccgccg cggccgcgtc gagcgagaag ctcgattacg agctccttca gcgctggatt
     1861 cgcttcctgg ccaagcctcc gaagttctat ccctacctga aagactggca ggcgatgatc
     1921 aaggacggcg gctccgagga cgaggcaaag tccctcgcgt cgacgttcca gggtctcgtg
     1981 accgatgtga tgttcgaggc gcgcgagatc aaggacgaga acgacatcat ccgcgccaag
     2041 gcgctgccgg gtaccaagaa gaaggagaag gcgaatctcc cgaacgagtt cgtgaccaac
     2101 gacgacttct gtccgggctg cggcctcgag ttgaagagcc tctcgggcga tcgcgtgttc
     2161 ctctacgccg acatgttccg tggcgatctc gtcgacagcg ccgatcccgc caatctcaag
     2221 gaagacgaca agcccggtct gttcgtgttc aagggaccgg cgctcgatcg ctggcttggc
     2281 gccgaccggc gtcgctacgt cgaggatctg cgggaggaca tcgagtcgct tggcaaggcg
     2341 atgcccccga agtactcata tgtgcacggt gtcgccgacg gggcaaaggc gcagacgatg
     2401 aa
//
LOCUS       DASRWS010000114         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_623432, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000114 DASRWS010000000
VERSION     DASRWS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623432"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFF72_02025"
     CDS             complement(<1..591)
                     /locus_tag="VFF72_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HZV91962.1"
                     /translation="MPDSKPLDPALREALESVTTATLTTVLLKKGLRNVWIRGAAPLG
                     PAAAQGRRAGRAFTLRFVPAREDLATPASWGSPISTRAAIEAIPEGAVAVVDAMGVAH
                     AGIFGDILCARMKKRGVAALVSDGVVRDVAGVLSTGLPVWCRGTASPASVAGLTFVGW
                     QEPIGCGGVAVMPDDVVVADLDGAVVVPAALLDEVVV"
     gene            813..1457
                     /locus_tag="VFF72_02030"
     CDS             813..1457
                     /locus_tag="VFF72_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HZV91963.1"
                     /translation="MADDRDQLLLFGSPSRPGSTLPSGWDYRENFVEAAEEASLIEAI
                     AALPLEEAVYRGYTARRRVARFGLAYDLDEQRERPAPRLPTIFEPLRTRAAAWIGQPP
                     EALVAMLVAEYRPGVPLGWHRDAPDFETVVGVSLAGWARMRFRRYPPMQPKKTDVVSL
                     DLAPRSAYVLRAEARWGWQHSVAPTPALRYSITLRTRRACRRKRATSRVALAAS"
     gene            complement(1469..>2095)
                     /locus_tag="VFF72_02035"
     CDS             complement(1469..>2095)
                     /locus_tag="VFF72_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZV91964.1"
                     /translation="SLGIAALFHFVPNTHVRWRHALAGGAFVAIGFALSKRLLAIYFG
                     TVPTYSMIYGAFAAVPIFLIWIYLSWIIVLLGAILAAYAPVARMQVRRWPPAPGSRFH
                     LALMMLRALVAERAAGRPGLTDIELARRLGTDPLQVQPLLDALVEIDWVGRLDEAGNA
                     RYVLVCDPQRVVAEALVGKLLLDPSPDLAGFWRQAHFAEMRLDEILRE"
BASE COUNT          344 a          737 c          733 g          281 t
ORIGIN      
        1 gacgacgacc tcgtcgagca gcgcggccgg cacgacgacc gcgccgtcga ggtcggcgac
       61 gacaacgtca tcgggcatga cggcgactcc gccgcagccg atcggctcct gccagcccac
      121 gaacgtgagg ccggcgaccg acgcaggcga cgccgtgccg cgacaccaca cgggcaggcc
      181 ggtcgagagc acgccggcga cgtcgcgcac gacgccgtcg ctcaccaggg ccgcgacgcc
      241 gcgtttcttc atgcgtgcgc aaaggatgtc gccgaagatg ccggcatggg caacgcccat
      301 cgcgtcgacg acggcgacgg cgccttcggg aatcgcctcg atcgccgcgc gcgtcgagat
      361 cggcgatccc cacgacgccg gcgtcgcgag gtcctcgcgg gcgggcacga agcgcagggt
      421 gaaggcgcgt ccggcgcggc ggccctgcgc ggcggccggg ccgagcggcg cggcgccgcg
      481 aatccagacg ttgcgcaggc ccttcttgag gaggacggtg gtgagcgtcg ccgtcgtcac
      541 cgactccagc gcttcccgaa gggcgggatc caggggcttc gaatcaggca tcggggcagc
      601 attggaattg cgttgcgtca gggtatagca cgccgaggtg ccgccgttcg acgcgaaggc
      661 cgggcattgc cccatccgag gcgaaggcca tcgcgatgtc gagcgcatgt cgacgatcat
      721 gacgtgaatc gccaccacga ttcgaccatc atgccgtgcg ggccgatcag gcgcggtcgc
      781 cgcgccgcag ttcgccgccg gacaatccac cgatggccga cgaccgcgac caactcctcc
      841 tgttcggctc gccgtctcgg cccggttcga cgcttccctc aggttgggac taccgcgaga
      901 acttcgtcga agccgccgag gaagcttcgc tgatcgaggc gatcgcggcg ctgccgctcg
      961 aggaggcggt ctatcgcggc tacacggcgc ggcggcgggt ggctcgcttc ggcctcgcct
     1021 acgacctcga cgaacagcgc gagcggcccg cgcctcgcct gccgacgatc ttcgagccgc
     1081 tgcgcacgcg cgccgctgcc tggatcggcc agccgccaga agcgctggtc gccatgctcg
     1141 tcgccgaata tcgccccggt gttccgctcg gctggcatcg cgacgccccc gatttcgaga
     1201 ccgtggtcgg cgtgtcgctc gccgggtggg cacggatgcg atttcgtcgg tatccgccca
     1261 tgcagccgaa gaaaaccgac gtcgtctcgc tcgacctcgc gccacgctcg gcctacgtgc
     1321 tgcgcgccga agcgcgctgg ggctggcagc acagcgtcgc gccgacgcca gcgttgcgct
     1381 attcgatcac cctgcgcact cgacgcgcct gtcgcaggaa gcgcgcgacg tcgcgcgtgg
     1441 ctttggccgc gtcttgacga tcggcgagtc actcccgcag gatctcgtcc aggcgcatct
     1501 ccgcgaagtg cgcctgtctc cagaagccgg caagatcggg cgacggatcg agcagcagct
     1561 tgccgaccag cgcctcggcc acgacccgct gcggatcgca gacgaggaca tagcgcgcat
     1621 tgcctgcctc gtcgagcctg ccgacccagt cgatttcgac aagcgcgtcg agcaacggct
     1681 ggacctgcaa ggggtcggtg cccagccgcc gcgccagctc gatgtcggtc agcccgggac
     1741 ggcccgccgc cctctcggcg accagcgcgc gcagcatcat gagtgccagg tggaagcgtg
     1801 aaccgggcgc cggtggccag cggcgcacct gcatgcgcgc caccggcgca tacgcggcga
     1861 ggatcgcgcc aaggaggacg atgatccagc tgaggtagat ccagatgaga aagatcggga
     1921 cggccgcgaa cgcgccgtag atcatcgaat aggtcggcac ggtgccgaag tagatcgcca
     1981 gaagacgctt cgacaaggcg aagccgatcg ccacgaaggc gccgccggcc agggcgtggc
     2041 gccagcgcac gtgggtgttc ggaacgaaat ggaagagcgc ggcgatgccc agcga
//
LOCUS       DASRWS010000115         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_903930, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000115 DASRWS010000000
VERSION     DASRWS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903930"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFF72_02040"
     CDS             complement(<1..696)
                     /locus_tag="VFF72_02040"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HZV91965.1"
                     /translation="MIQHLVDPLRRSLLAGAAVFLPTLAVAARRVDADADPSPSAPLL
                     AMLWDERIDPEPYLVSEKYDGVRAVWDGRLLRHRSGRTVSAPATFLRSLPDEAIDGEL
                     WLGRGRFDALSAIVRRHEPRPDEWAAVRYMAFEMPQASGSFADRADRLAALLGQRPRS
                     PVEAAPQRHVANRDALMRLLGSVVAGGGEGLMLHLASSPARSGRSADLLKLKPHLDAE
                     AVVCWHPGTGRNRS"
     gene            881..3505
                     /gene="leuS"
                     /locus_tag="VFF72_02045"
     CDS             881..3505
                     /gene="leuS"
                     /locus_tag="VFF72_02045"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZV91966.1"
                     /translation="MDPEFDPRGIEKRAQSAWTAADAYRVEEIATRPKFYACSMLPYP
                     SGRLHMGHVRNYTINDMLARHLRMTGMNVLMPMGWDAFGLPAENAAMKNGVPPARWTR
                     DNIAAMKAQMQSMGLAIDWSREIATCDPSYYKWNQWLFLKMLEAGIAERRTQTVNWDP
                     VDQTVLANEQVVDGRGWRSGAIVEKREVPGYYLKITKYAQELLDCVQTGLPGWPERVK
                     TMQENWIGRSEGVRFAFPHTIASDDGALIGGGRLWVFTTRADTIMGVTFCAVAPEHAL
                     AVHAAATRPEVAAFLEECKAGGTTEAELATQQKKGIFTGLFVEHPLSRQPIPLWVGNY
                     VLIGYGDGAVMGVPAHDERDFAFARQYGIDLLQVIHIDGKHFSYDHWQDWYAETDGAV
                     TINSDNYSGLDHVAAVDAVAAALAHHGLGGKRTVWRLRDWGISRQRYWGTPIPIVHCE
                     GTPGSPGCGVVPVPEQDLPVVLPDNLIPDGSGNPLNKSEAFLACACPRCGRPARRETD
                     TMDTFVDSSWYFMRYCDAHLEDAMVGAGTAYWMPMDQYIGGIEHAILHLLYARFWTKV
                     MRDLKLVDIDEPFTRLLTQGMVLNHVWARQTPQGGIEYFPPDEVTPVDSGEGRPSEGR
                     LADGRIVGYRGMLKMGKTERNGVDPQDIIDKYGADTARLFVMFASPPEQTLDWSNAGV
                     EGANRFLKRLWKFAARHAGAVTAAAGRQAQGASALRHEVHTVLRQVSYDYERMQYNTV
                     VSGAMKLLNALESAADGDPPALREGLGILLRVLYPVCPHVTWAIWGELGFDVERGPLL
                     DASWPSVDERALVQAEIELVLQVNGKLRGSLRVPATTDKAGIEAAALATPEVARFAQG
                     GSIKRVIVVPGRLVNVVV"
     gene            3505..3996
                     /gene="lptE"
                     /locus_tag="VFF72_02050"
     CDS             3505..3996
                     /gene="lptE"
                     /locus_tag="VFF72_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZV91967.1"
                     /translation="MPTRRSLVLSLAATLGGCGFTLKRPPELRFRTIQLTGFAVRSPI
                     AAELTRDIDASATTRVVASLKDAQVVLQALAEEREKSVVATTAANLVREYELRSRFRF
                     TLRSASGKDLVPSSEILLKRELTYTESAALGKEQEEAFLYRAMESDIVSQVLRRLASV
                     QVL"
     gene            3996..>4681
                     /gene="holA"
                     /locus_tag="VFF72_02055"
     CDS             3996..>4681
                     /gene="holA"
                     /locus_tag="VFF72_02055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZV91968.1"
                     /translation="MQVRPDALARQLERGLAPMYTVWGDEPLLAQEACDAIRAAARAG
                     GCSERQVHIVSGAHFDWSSLLGAAQALSLFADRQLVEIRIPSGKPGKEGSQALQRYCE
                     AAAAGGGRDVITLVQLPRLDGMQQKSAWFNALDRVGPTVRVSAIERNALPAWIAQRLS
                     AQGQAVPSGPEGQQALAFFADRVEGNLLAAHQEIQKLGLLYPQGELSFEQIESAVLNV
                     ARYDVFKLGEA"
BASE COUNT          763 a         1605 c         1628 g          685 t
ORIGIN      
        1 gctccggttc ctgccggtgc cgggatgcca gcagacgacg gcctcggcat cgagatgggg
       61 cttgagcttc agcaagtcgg cgcttcgccc gcttcgagcg ggggacgacg cgaggtgcag
      121 catcagccct tcgccgccac cggcgacaac gctgccgagc aggcgcatca aggcgtcgcg
      181 gttggcgacg tggcgctgcg gtgcggcctc gaccggtgac ctcggccgtt ggccgagcaa
      241 cgccgcgagc cggtcggcac ggtcggcgaa gctgccgctc gcctgcggca tctcgaacgc
      301 catgtagcgc accgccgccc attcgtcagg ccgcggctcg tggcggcgga cgatcgccga
      361 gagcgcatcg aagcggccgc ggcccagcca cagctcgccg tcgatcgcct cgtccggcag
      421 cgatcgcaag aaggtggctg gcgcggacac cgtcctgccg cttcggtggc gcagaagccg
      481 gccgtcccag accgcccgca cgccatcgta tttttcgctg accagatagg gctcgggatc
      541 gatgcgctcg tcccagagca tggcgagaag cggcgcgctc ggcgacggat ccgcatcggc
      601 gtcgacacgc cgggcggcga cggcgagcgt cggcagaaag accgcggcgc cggcgagcag
      661 cgagcggcgc agcggatcga cgaggtgctg gatcatggca gcgggccgga acggcgatgg
      721 acgcgaaggc gccatcgtcg acgtcggcga ggctctcgtc gagagcctcc gtcgggggcg
      781 ccggttccct cggcagggcg atgcacgccg cgcgcgaggc ggcgtcgaaa ccgatcggct
      841 ttctataatc gatcgatgcc tgcccgtcgc gtcgtctttc atggatcctg aattcgaccc
      901 gcgcgggatc gagaagcgcg cccaatcggc atggaccgcg gccgacgcgt atcgcgtcga
      961 ggaaatcgcg acgcgtccga agttctacgc ctgctcgatg ctgccgtatc ccagcggccg
     1021 cctgcacatg gggcatgtgc gcaactacac catcaacgac atgctggcgc gccacctgcg
     1081 catgaccggc atgaacgtcc tgatgccgat gggctgggat gccttcggcc tgccggccga
     1141 gaacgcggcg atgaagaacg gcgtgccgcc ggccaggtgg acgcgcgaca acatcgcggc
     1201 gatgaaggcg cagatgcagt cgatgggcct ggccatcgac tggagccgcg agatcgcgac
     1261 ctgcgacccg agctactaca agtggaacca gtggttgttc ctgaagatgc tcgaggcggg
     1321 catcgccgag cgacggactc agaccgtgaa ctgggatccg gtcgaccaga cggtgctggc
     1381 caacgagcag gtcgtcgatg ggcgcggctg gcgatcggga gcgatcgtcg agaagcgcga
     1441 ggtgccgggc tactacctga agatcacgaa gtacgcgcag gagctgctcg actgcgtgca
     1501 gaccggcctg cccggctggc ccgaacgcgt caagacgatg caggagaact ggatcggtcg
     1561 cagcgaaggc gtccgattcg cctttccgca caccatcgcc agcgacgacg gcgcgctgat
     1621 cggcggtggc aggctgtggg tcttcacgac ccgggccgac acgatcatgg gcgtgacctt
     1681 ctgcgccgtc gcgcccgagc acgcgctggc cgtgcacgcc gccgcgacgc ggcccgaggt
     1741 cgcggcgttc cttgaggagt gcaaggccgg cgggacgacc gaggccgagc tggcgacgca
     1801 gcaaaagaag ggcatcttca cggggctctt cgtcgagcat ccactcagcc gccagccgat
     1861 tcccctctgg gtcggcaact acgtgctgat cggctatggc gacggtgccg tgatgggcgt
     1921 gccggcgcac gacgagcgcg acttcgcctt cgcgaggcag tacggcatcg acctgctgca
     1981 ggtcatccac atcgacggca agcacttcag ctacgaccac tggcaggact ggtacgccga
     2041 gaccgacggc gccgtcacca tcaattccga caactacagc ggcctcgatc acgtcgccgc
     2101 cgtcgatgcc gtcgccgcgg cgctggcgca ccacgggctg ggcggcaagc gcacggtctg
     2161 gcgattgcgc gactggggca tcagccgcca acgctactgg ggcacgccga tcccgatcgt
     2221 ccattgcgag ggcacgccgg gttcacccgg ctgcggcgtc gtcccggtgc ccgagcagga
     2281 cctgccggtc gtgctgccgg acaacctcat ccccgacggc agcggcaacc cgctcaacaa
     2341 gagcgaggcg ttcctcgcgt gcgcctgtcc gcgctgcggc cggcccgcgc gccgcgagac
     2401 cgacacgatg gacaccttcg tcgacagctc gtggtacttc atgcgctact gcgacgcgca
     2461 tctcgaggac gcgatggtgg gcgccggcac cgcctactgg atgccgatgg accagtacat
     2521 cggcggcatc gagcacgcga tcctgcacct gctgtacgcg cgcttctgga ccaaggtgat
     2581 gcgcgacctg aagctggtcg atatcgacga gccgttcacg cgcctgctga cgcaggggat
     2641 ggtcctcaac cacgtctggg cgcggcagac gccgcaaggc ggcatcgaat acttcccgcc
     2701 cgacgaagtc acgccggtcg acagcggcga gggccgtccg agcgagggca ggctcgccga
     2761 cggccgcatc gtcggctatc gcggcatgct caagatgggc aagaccgagc gcaacggcgt
     2821 cgacccgcag gacatcatcg acaagtacgg tgccgacacg gcacgcctgt tcgtcatgtt
     2881 cgcttcgccg cccgagcaga cgctcgactg gagcaatgcc ggcgtcgaag gcgcgaatcg
     2941 attcctgaag aggctgtgga aattcgcggc ccgccacgcc ggcgcggtga ccgccgcggc
     3001 tggccggcag gcacagggtg cttcggccct gcgtcacgag gtccatacgg tgctgcgcca
     3061 ggtgagctac gactacgagc gcatgcagta caacaccgtc gtctcggggg cgatgaagct
     3121 gctcaacgcg ctcgaatccg ccgccgacgg cgatccgccg gcgctgcgcg agggcctggg
     3181 catcctgctg cgggtcctct acccggtctg tccgcacgtc acctgggcga tctggggcga
     3241 gctggggttc gatgtcgagc gcgggccgct gctcgatgcc tcctggcctt ctgtcgacga
     3301 gcgcgcgctc gtgcaggcag agatcgagct cgtgctgcag gtcaacggca agctgcgcgg
     3361 cagcctccgc gtgccggcga ccaccgacaa ggccggcatc gaggccgcgg ccctggccac
     3421 cccggaggtg gcgaggttcg cgcagggcgg cagcatcaag cgggtgatcg tggttccggg
     3481 gcggctggtc aacgtcgtcg tctgatgccg acgcgccgct cgctcgtcct ctcgttggcg
     3541 gcgacgctcg gaggctgcgg cttcacgctc aagcgccctc ccgagctgcg ctttcgcacc
     3601 atccagctca ccggattcgc ggtgcgatcg ccgatcgccg ccgagctcac tcgcgacatc
     3661 gacgccagcg cgacgacccg ggtcgtcgct tcgctgaaag atgcccaggt cgtcctgcaa
     3721 gcgctggccg aggaacgcga gaaaagcgtc gtcgcgacca cggccgccaa cctggtccgc
     3781 gaatacgagc tgcgctcgcg tttccgcttc acgctgcgca gcgcctcggg caaggatctc
     3841 gtgccgtcga gcgaaatcct gctgaagcgg gagctcacct ataccgagag cgcagcgctc
     3901 ggcaaggagc aggaagaagc ttttctctac cgtgcgatgg aaagcgacat cgtctcgcag
     3961 gtgctgcgcc gcctggcgtc ggtgcaggtg ctctgatgca ggttcggccc gacgcgctcg
     4021 ccaggcagct cgaacgcggc ctcgcgccga tgtacacggt gtggggcgac gagcccctgc
     4081 tcgctcagga agcgtgcgac gcgatccgcg cggcggcacg cgccggcggc tgcagcgagc
     4141 ggcaggtgca catcgtctcc ggcgcccact tcgactggag cagcctgctc ggcgccgccc
     4201 aggcgctcag cctcttcgcc gaccgccagc tcgtcgagat ccgcatcccg tcgggcaagc
     4261 ccggcaagga aggctcgcag gcactgcagc gctattgcga ggccgccgcg gccggtggcg
     4321 gccgcgacgt gatcacgctc gtgcagttgc cgcggctcga cggcatgcag caaaagagcg
     4381 cctggttcaa cgcgctcgat cgtgtcggcc cgaccgtgcg ggtcagcgcc atcgagcgca
     4441 acgcgctgcc ggcatggatc gcgcagcgtc tgtcggcgca ggggcaggcc gtcccttccg
     4501 gccccgaggg gcaacaggcg ctcgccttct tcgccgatcg cgtcgaaggc aatcttctcg
     4561 ccgcgcatca ggaaatccag aagctcggcc tgctctatcc gcagggcgag ctgagcttcg
     4621 agcagatcga gtcggcggtg ctcaacgtcg cgcgctacga cgtcttcaag ctcggcgagg
     4681 c
//
LOCUS       DASRWS010000116         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_623805, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000116 DASRWS010000000
VERSION     DASRWS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623805"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1213)
                     /gene="ccmI"
                     /locus_tag="VFF72_02060"
     CDS             complement(5..1213)
                     /gene="ccmI"
                     /locus_tag="VFF72_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HZV91969.1"
                     /translation="MDAFLASARKRLAELRVRRDAGTLAAADYEVQRRAIEREMSEHL
                     LADVDSAAVRPSARLWAGVAVFVLAVAAAGYWKTGSPSLAGAGPDRSSTLSAAAGGAS
                     AASGLQQIAAMVDKLAARMKERPDDAQGWLMLARSYSVLGRFAEAIPAYKRATELQPD
                     NAVLLADYADAVAASRGGVDNPESEQLVKRALAADPKQPKALALAGTIAFDRGDYKGA
                     IDRWQQMAEVLPADSAMLKQVQASIAEARQRAGAPAAAAAPGSAPLARVNDNAAATTP
                     SADAAPHLASGKSVSGTVTLAPGLAAQAAPGDTVFVFARAAGGGRMPLAVQRARVSDL
                     PLHYKLDDAMAMSPAATLSSAATVVVGARISKSGNAMPQAGDLAGEASPVAPGANDVD
                     IRIDSVLGAR"
     gene            complement(1219..1692)
                     /locus_tag="VFF72_02065"
     CDS             complement(1219..1692)
                     /locus_tag="VFF72_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZV91970.1"
                     /translation="MTPMRFLRGLAAALLAAAALAAAAKEAAPEAADPAVEARMHRVT
                     SELRCLVCQNQTIAESSSDLAADLRRETRELIKQGKTDRQIVDYMTARYGDFVLYRPP
                     LRATTVLLWFGPALMLLGGGGALLLVLRRRSRMTDAAFEPDDDDALLEGGSAAAR"
     gene            complement(1822..2361)
                     /locus_tag="VFF72_02070"
     CDS             complement(1822..2361)
                     /locus_tag="VFF72_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HZV91971.1"
                     /translation="MKRLWFLIPLAAFFALAAILARGLRLDPHEVPSPLINKPAPNFA
                     LTRLDDAHQTIRLDDMRGKVWMLNVWASWCVSCRAEHPLLVAFSKKHLVPIYGLDYKD
                     QRADATGWLTRLGNPYDVSLFDGEGRVGIDFGVYGVPETFIIDRDGVIRLKHIGPLTE
                     EALTTKIEPMLEKLKKSDA"
     gene            complement(2358..4343)
                     /locus_tag="VFF72_02075"
     CDS             complement(2358..4343)
                     /locus_tag="VFF72_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZV91972.1"
                     /translation="MIPELGHFALWLALGISATLGVMPMLGAARGRADWMALARPCAR
                     IVFALVAFSFVCLALAFAGSDFSVLYVAENSNRSLPMHYRLTAVWGGHEGSILLWLLM
                     LTFWTFAVAQFSRHLPPKVVARILAVMGWLAFGFLLFILITSNPFARLSPVPADGRDL
                     NPLLQDPGMVIHPPMLYMGYVGLSVAFSFAVAALIGGNLDSTWARWTRPWTTAAWIFL
                     TIGIALGSGWSYYVLGWGGWWFWDPVENASFMPWLVATALIHSLAVTEKRNSFKNWTV
                     LLAILAFSLSLLGTFLVRSGVLSSVHAFATDPRRGLFILVFLVVVIGASLTLYAWRAP
                     TVGLGARFALLSRESLLLGNNVLLVVACGAVLLGTLYPLALDALGLGKISVGPPYFDT
                     VFVPLLTPLIFLMGVGPMARWKQSELPDLARRLRWAAGASVVATLLVVWAKGGIGVVG
                     VIGLLMATWIVASVATDLVERLRPPAGFRSSVLQRARQIPPAMLGMMAAHLGIAIFIF
                     GVTMVKTGEVERDVRMNIGDTTEIHGYTFTFRGVQEATGPNYRAVQGRIDVTEGDRAV
                     TSLHPEKRFYPSTESTMTEASIDSGLTRDLYVSLGEPVAGGLAWIVRVYYKPFVRWIW
                     GGCFVMALGGLLAMSDRRYRARSRQTSPATAAVAASA"
     gene            complement(4340..4807)
                     /gene="ccmE"
                     /locus_tag="VFF72_02080"
     CDS             complement(4340..4807)
                     /gene="ccmE"
                     /locus_tag="VFF72_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZV91973.1"
                     /translation="MKPRHKRLALIATGVAVLGAAVALVLNAFQSNLVFFYSPSQVVA
                     HEAPAARTFRIGGLVERGSLKRDGIHVSFVVTDTAKSIPVSYDGILPDLFKEGKGVVA
                     QGRLQDGVFVAHEVLAKHDENYMPPEAADALKKAQQGNPKLAETLAGEPAKQP"
     gene            complement(4804..4968)
                     /gene="ccmD"
                     /locus_tag="VFF72_02085"
     CDS             complement(4804..4968)
                     /gene="ccmD"
                     /locus_tag="VFF72_02085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZV91974.1"
                     /translation="MTWHSAGDFFAMGGYGLYVWGAYGVAAACMAIEPVLAALRLARA
                     RRALRAGAAG"
BASE COUNT          816 a         1696 c         1855 g          682 t
ORIGIN      
        1 tctgtcagcg cgcgccgagc acgctgtcga tccggatgtc gacgtcgttc gcgccgggcg
       61 ccaccgggct ggcttcgccg gccaggtcac ccgcttgcgg catcgcattg ccgctcttgc
      121 tgatccgcgc gccgacgacg acggtcgcgg cgctcgagag cgtcgcggcc ggcgacatgg
      181 ccatcgcgtc gtcgagcttg tagtgcagcg gcaggtcgct cacgcgggcg cgctgcacgg
      241 ccagcggcat ccggccgccg ccggcggcgc gggcgaagac gaagaccgtg tcgcccggcg
      301 cggcctgggc ggcgagtccg ggcgccagcg tcacggtgcc gctcacgctc ttgcccgacg
      361 cgaggtgcgg cgcagcgtcg gccgaaggcg tcgtcgccgc ggcgttgtcg ttgacccggg
      421 cgagcggggc cgagcccggc gctgctgccg ctgccggcgc gccggcgcgc tggcgcgcct
      481 cggcgatgct cgcctgaacc tgcttcagca tggcgctgtc ggccggcagc acctcggcca
      541 tctgctgcca gcgatcgatg gcgcctttgt agtcgccacg gtcgaaggcg atggtgccgg
      601 cgagcgcgag cgccttgggc tgcttcgggt cggcggccag ggcgcgtttc acgagctgct
      661 ccgattccgg gttgtcgacg ccgccgcggc tggcggccac ggcatcggcg tagtcggcga
      721 gcagcacggc gttgtccggc tgcagctcgg tggcccgctt gtaggcgggg atcgcctcgg
      781 cgaaacgccc gagcacgctg tacgagcggg cgagcatcag ccagccttgc gcgtcgtccg
      841 gccgctcctt catgcgcgcg gcgagcttgt cgaccatggc ggcgatctgc tgcagtccgc
      901 tggccgcgct cgcaccacct gcggcggcgg acagcgtcga cgagcgatcg ggaccggcgc
      961 cggccagcga gggcgagccg gtcttccagt agccggctgc tgccaccgcc agcacgaaca
     1021 cggcgacgcc ggcccacagc cgggccgagg ggcggaccgc ggcggaatcg acgtcggcca
     1081 gcagatgctc gctcatctcg cgctcgatgg cgcgacgttg cacttcgtag tcggcggcgg
     1141 cgagggtgcc ggcatcgcgg cgaacccgca gttcggcgag gcgcttgcgc gcagaggcga
     1201 ggaaggcgtc catcgttgtc agcgggcggc agcgctgccg ccttcgagca gcgcgtcgtc
     1261 gtcgtcgggt tcgaacgccg cgtcggtcat gcggctgcgc cggcgcagca ctaggagcag
     1321 cgcgccgccg ccgccgagca gcatcagcgc cgggccgaac cagagcagca cggtggtcgc
     1381 gcgcagcggc ggccgataga gcacgaaatc gccgtagcgc gcggtcatgt agtcgacgat
     1441 ctggcggtcg gtcttgccct gcttgatcag ctcgcgcgtc tcgcgccgca ggtcggcggc
     1501 aaggtcggag ctggattcgg caatcgtctg gttctggcag acaaggcagc gcagctcgga
     1561 cgtgacgcga tgcatgcgcg cctcgacggc cgggtcggct gcttccggcg cggcttcctt
     1621 ggcggcggcg gcgagtgccg cggcggcgag caacgctgcc gcgaggccgc gcaggaaacg
     1681 catcggcgtc atctcgaacg ctcgactcgt cggtcgatgc cgaaggcatg gatccggcgc
     1741 cagcgagcac gaggcccgtc cacgtgtatc gccgtttcgc gctcgcgccc gtcagcgagc
     1801 ggagcgagga tgggggctcc atcacgcatc ggacttcttc agcttctcga gcatcggttc
     1861 gatcttggtc gtcagcgctt cctcggtgag cgggccgatg tgcttgagtc ggatgacgcc
     1921 gtcccggtcg atgatgaacg tctcgggaac gccgtagacg ccgaagtcga tgccgacccg
     1981 gccctcgccg tcgaacagcg agacgtcgta ggggttgccg aggcgggtga gccagccggt
     2041 cgcgtcggcg cgctggtcct tgtagtcgag cccgtagatc ggcaccagat gcttcttcga
     2101 gaaggcgacc agcagcgggt gctcggcacg gcacgagacg caccacgacg cccagacgtt
     2161 gagcatccac accttgccgc gcatgtcgtc gaggcggatc gtctggtgcg catcgtcgag
     2221 gcgggtcagc gcgaagttcg gcgccggctt gttgatgagg ggcgacggca cctcgtgcgg
     2281 gtcgaggcgc aggccgcgcg ccaggatcgc ggcgagcgcg aagaaagccg ccaggggaat
     2341 gaggaaccag agccgcttca tgccgatgcc gcgacagcgg ccgtggccgg cgacgtctgc
     2401 cgggagcggg cgcggtagcg ccggtcgctc atggcgagca ggccgcccag cgccatcacg
     2461 aagcagccgc cccagatcca gcgcacgaag ggcttgtagt agacgcgcac gatccaggcg
     2521 agaccgccgg cgacgggctc gccgagcgac acgtagaggt cgcgggtcag ccccgagtcg
     2581 atcgaggctt cggtcatcgt cgactcggtc gacgggtaga agcgcttctc ggggtgcaac
     2641 gacgtcacgg cgcgatcgcc ctcggtcacg tcgatccgtc cctgcacggc gcggtaattc
     2701 ggccccgtcg cttcctgcac gccgcgaaag gtgaacgtgt agccgtggat ctcggtggtg
     2761 tcgccgatgt tcatccgcac gtcgcgctcg acttcgccgg tcttcaccat ggtgacgccg
     2821 aagatgaaga tcgcaatgcc caggtgggcg gccatcatgc cgagcatcgc cggcgggatc
     2881 tgccgcgccc gctgcagcac gctgctgcga aagcccgccg gcggccgcag gcgctcgacc
     2941 aggtcggtgg cgaccgaggc gacgatccat gtcgccatca gcaggccgat gacaccgacc
     3001 acgccgatgc cacccttggc ccagacgacg agcaacgtcg cgacgacgct ggcgccggcg
     3061 gcccagcgca gtcgacgtgc gaggtcgggc agctcggatt gcttccagcg cgccatcgga
     3121 ccgacgccca tgaggaagat cagcggtgtc aggagcggca cgaacacggt gtcgaaatag
     3181 ggaggcccga ccgagatctt gccgagtccc agagcatcga gtgcgagcgg atagagcgtg
     3241 cccagcagca ccgcgccgca ggcgacgacg agcagcacgt tgttgccgag gaggagcgat
     3301 tcacgcgaca gcagcgcgaa gcgggcaccc agtcccaccg tgggagcgcg ccacgcatag
     3361 agggtcagcg aggcgccgat gacgacgaca aggaacacga ggatgaagag gccgcgccgc
     3421 ggatcggtgg cgaaggcgtg caccgacgaa agcacgcccg agcgaaccag gaaggtgccg
     3481 agcagcgaca gcgagaaggc gaggatggcg agcagcaccg tccagttctt gaagctgttg
     3541 cgcttctcgg tgacggcgag cgaatggatc agcgccgtcg cgaccagcca gggcatgaag
     3601 gaggcgttct cgaccgggtc ccagaaccac cagccgcccc agccgagcac gtagtacgac
     3661 cagccgctgc cgagggcgat gccgatggtg aggaagatcc aggccgccgt cgtccagggc
     3721 cgtgtccagc gcgcccaggt cgagtcgagg ttgccgccga tcagcgccgc gacggcgaac
     3781 gagaacgcca ccgacaggcc gacatagccc atgtagagca tcggcggatg gatgaccatg
     3841 cccgggtcct gcagcagcgg gttgaggtcg cgaccgtcgg cgggcaccgg ggagagccgc
     3901 gcgaacggat tcgatgtgat gaggatgaag aggaggaacc cgaaggcgag ccagcccatc
     3961 accgcgagga tgcgagcgac gaccttgggc ggcaggtggc ggctgaactg ggcgaccgcg
     4021 aaggtccaga acgtcagcat cagcagccag agcaggatcg agccctcgtg gccaccccac
     4081 acggcggtca gccggtagtg catcggcagc gagcggttcg agttctcggc gacgtagagc
     4141 accgagaaat cgctcccggc gaaagccagc gccaggcaga cgaacgagaa ggcgacgagc
     4201 gcgaagacga tgcgcgcaca cgggcgcgcc agcgccatcc agtcggcccg gccgcgcgcc
     4261 gcgccgagca tcggcatgac gccgagcgtc gcggaaatgc cgagagcgag ccagagcgcg
     4321 aagtgaccga gttcggggat catggctgct tcgccggctc gccggccagc gtctccgcga
     4381 gtttgggatt gccctgctgc gccttcttca gcgcgtcggc cgcctccggc ggcatgtagt
     4441 tctcgtcgtg cttggcgagc acctcgtggg cgacgaagac gccgtcctgc aggcgaccct
     4501 gggcgacgac gcccttgcct tccttgaaga ggtcgggaag gatgccgtcg tagctcaccg
     4561 ggatcgactt ggcggtgtcg gtgacgacga agctcacgtg aatgccgtcg cgcttgaggc
     4621 tgccgcgctc gaccaggccg ccgatgcgaa atgtccgcgc cgcgggcgcc tcgtgcgcga
     4681 ccacctgcga aggcgaatag aagaagacca ggttgctctg gaaggcgttg agcaccaggg
     4741 cgacggcggc gcccagcacc gcgacgccgg ttgcgatcag cgccagccgc ttgtgccggg
     4801 gcttcatcca gccgctccgg cacgaagcgc tcggcgggcg cgcgccaggc gcagcgcggc
     4861 aagcaccggc tcgatcgcca tgcaggccgc cgcgacgccg taggcgcccc agacgtagag
     4921 gccgtagcca cccatggcga agaaatcgcc ggcgctgtgc caggtcatgt cgccgctccc
     4981 tgtgcgtcga cccggcgccg gacgccgacc gcttcggatg cccactgggc gccacgctcg
     5041 cgctcgagg
//
LOCUS       DASRWS010000117         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2635550, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000117 DASRWS010000000
VERSION     DASRWS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635550"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFF72_02090"
     CDS             <1..414
                     /locus_tag="VFF72_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZV91975.1"
                     /translation="FIVGFPGETAGDFEKTMALVEEIGFDHSFSFLFSPRPGTPAATL
                     DDDTAPAEKNVRLQRLQAALEIQGKAISQSRVGTVRRVLVEGTSRRNAGELSGRTSCN
                     RVVNFAAPDRLVGTMADVRITEVQGHSLRGEVVVA"
     gene            complement(440..724)
                     /locus_tag="VFF72_02095"
     CDS             complement(440..724)
                     /locus_tag="VFF72_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91976.1"
                     /translation="MPIADAFWHIANFFSPSLSVAVIAASAAKLLWRRELAAVSWQRL
                     VICSAIPMALVAAAGLVIFERDGKMVTYVAMVCVCAASLWWAGFRRPSAR"
     gene            764..2047
                     /gene="hemA"
                     /locus_tag="VFF72_02100"
     CDS             764..2047
                     /gene="hemA"
                     /locus_tag="VFF72_02100"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZV91977.1"
                     /translation="MSVFALGLNHTTAPLDLRGRFAFAGPQLPAALQSFRSRHERVNE
                     VAIVSTCNRTELYLGADIAAADPALDWLAGVGGVASNTLRSHAYILEGPSAARHAFRV
                     ASGLDSMVLGEPQILGQLKHAVREADSAGTLGTTLQQMFQRSFAVAKEVRSSTEIGNH
                     SISMAAAAVRLASQLFESIAQTRVLFVGVGEMIELAATHFAARAPRAMTIANRSVERA
                     AELAARLGATAMPLADLPVRLAEFDIVVSSTASALPIIGLGAVERALKARRHRPMFML
                     DLAVPRDIEAEVARLSDIYLYTVDDLSALVQTAGQKREAAVAQAEAIIENGVQSFVQW
                     LDRRGSIPLIQALHSQADDWRSLEIARARRALAGGEDIGAVLEQLSRGLTQKMLHGTF
                     AELRAAEGDERNELARTVSRLFLRTRTRSSPDDKR"
BASE COUNT          364 a          710 c          700 g          338 t
ORIGIN      
        1 ttcatcgtcg gctttccggg cgagacggcg ggcgacttcg aaaagaccat ggcgctggtc
       61 gaagagatcg gcttcgacca cagcttcagc ttcctgttca gcccgcgtcc cggcacgccg
      121 gctgccactc tcgacgacga cacggcacct gccgaaaaga acgtgcggct gcagcggctg
      181 caggcagccc tggaaatcca gggaaaagcg atcagccagt ctcgcgtcgg cacggttcgg
      241 cgtgttctgg tcgaaggaac ctcgcgcagg aacgccggcg agctttccgg tcgcaccagt
      301 tgcaatcgcg tcgtcaattt cgcggcaccg gatcggctgg ttggaacgat ggctgacgtg
      361 cgcatcaccg aggtgcaggg tcactcgctt cgcggcgagg tcgtggtggc ctgacgcgat
      421 tcggccagaa cgcggacact caacgcgccg aagggcgccg gaatcctgcc caccagagcg
      481 acgcggcaca gacgcagacc atcgcgacat acgtgaccat cttgccgtcg cgctcgaaga
      541 tcaccaggcc ggcagcggcc acgagcgcca tcgggattgc cgagcagatc acgagtcgct
      601 gccaggaaac tgccgcgagc tcgcgtcgcc acaggagctt ggcggccgac gcggcgatga
      661 ccgccaccga caacgaaggc gagaaaaagt tggcgatgtg ccagaaagcg tcggcaatgg
      721 gcatgagaag cttcgaaccg ggcgacgatt ttataatccg acgatgagcg tcttcgcgct
      781 ggggctcaat cacacgaccg ctccgttgga cctgcgcggg cgattcgcat ttgccgggcc
      841 gcagcttccg gccgcgctgc aatctttccg ctcgcgccat gagcgggtca acgaagtcgc
      901 catcgtctcg acctgcaatc gcaccgagct gtatctcggc gccgacatcg ccgcggcgga
      961 tccggcgctc gactggctgg ccggggtcgg cggcgtcgcc agcaacaccc tgcgctcgca
     1021 tgcctacatc ctcgaaggcc cctcggcggc gcggcacgcc tttcgcgtcg ccagcggact
     1081 cgattcgatg gttctcggcg agccccagat cctgggtcag ctgaagcacg cggttcgcga
     1141 ggccgacagc gccgggacgc tcggaacgac gctgcagcag atgttccagc gttccttcgc
     1201 ggtcgccaag gaggtacgct catcgaccga gatcggcaat cattcgatca gcatggccgc
     1261 ggctgccgtc cgattggcgt cgcagctgtt cgagtcgatc gcgcagacgc gcgtcctctt
     1321 cgtcggtgtc ggcgagatga tcgagctcgc ggcaacccac ttcgcggcgc gcgcccctcg
     1381 cgcgatgacg atcgccaacc gcagcgtcga gcgtgccgca gagttggcgg cacggctggg
     1441 cgcgacggcg atgccgctgg ccgacctgcc ggtacggctt gccgagttcg atatcgtcgt
     1501 ttcgtcgacg gcgagcgcgc tgcccatcat cgggctgggc gcggtcgagc gagccctgaa
     1561 ggcacgtcgg caccggccga tgttcatgct cgacctcgcc gtgccacgcg acatcgaagc
     1621 cgaagtcgcg cggctgtcgg acatctacct ctacaccgtc gacgatctgt cggcgctggt
     1681 gcagaccgcc ggacagaagc gcgaagccgc cgtggcccag gccgaggcca tcatcgaaaa
     1741 cggcgtgcag agcttcgttc agtggctcga ccggcgtggc tcgattccgc tgatccaggc
     1801 gttgcactcg caggccgacg actggcgatc gctcgagatc gcgcgcgccc gcagggccct
     1861 ggcgggtggc gaagacatcg gcgccgtcct ggagcagttg tctcgcggac tgacgcagaa
     1921 gatgttgcac ggcacgttcg ctgaactgcg cgctgccgaa ggcgacgaac gcaacgagct
     1981 ggcgcggacc gtttcaaggc tcttcctgcg cacgagaacg cggtcgtcgc ccgacgacaa
     2041 gcggtagcga cgatcgtcgt cccgactgct gcgccgcctt cgcggcgcag tcttctcgcc
     2101 gatcccgact gc
//
LOCUS       DASRWS010000118         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_625144, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000118 DASRWS010000000
VERSION     DASRWS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625144"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFF72_02105"
     CDS             <1..1281
                     /locus_tag="VFF72_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZV91978.1"
                     /translation="AIGAESTLARIVRLVESAQEGKAPIQRLVDRVAAVFVPVVIGIA
                     LLTLLSWGLTTGNWPQAILDAVAVLVIACPCALGLATPTAIMAGTGVAARHGILIKDA
                     EALEVAHDLRIVAFDKTGTLSEGRPELVAFEAAIGSADALLATSASIQAGSEHPLARA
                     VVEAADRARLPVPIATEVRAIAGRGMSGRIGQRELRLGSGLFMGELGVDTSALAARSA
                     ALQSEGRSVSWLADVTAEPALVGLLAFGDRLKPSAANAVRALQALGVRAVLLTGDNRG
                     SAALAAQALGIADVRAEVLPEDKARIVGELKAGGDRVAMVGDGINDAPALAAADVGIA
                     MSSGTDVAMFAAGITLMRGDPSLVVDAIDISRRTYAKIRQNLFWAFAYNVVGIPLAAM
                     GLLSPVIAGAAMALSSVSVVTNALMLRRWKGRAQ"
     gene            1278..1697
                     /gene="cueR"
                     /locus_tag="VFF72_02110"
     CDS             1278..1697
                     /gene="cueR"
                     /locus_tag="VFF72_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HZV91979.1"
                     /translation="MSRTAIDPGSFNIGEAAARSGVSAKMVRHYESLGLLPKVARTES
                     GYRQYGEKEIHTLRFVRRARVLGFGMAEIADLLKLWQNRRRASADVKRIALAHVADLE
                     RRMDEMAAMKRTLERLADCCRGDDRPDCPILDELGEG"
     gene            complement(1816..2547)
                     /locus_tag="VFF72_02115"
     CDS             complement(1816..2547)
                     /locus_tag="VFF72_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HZV91980.1"
                     /translation="MFPPWFHALSLGALALGGACALAVGIDVVRRPQKMAVMNVVWPV
                     TALFGTVLTLWAYVRYGRASTGAATQKAAAGQRAMPTAAQPFAATVGKAALHCGAGCM
                     IGDVIAEWLAFAAPVVAVWLGWHTLFDEKIFADWVLDFLFAFALGIVFQYFAIVPMRN
                     LSPGKGIVAALKADTLSLVSWQIGMYGFMAFAQFLWFDHGFGAKAEVDTPEFWFAMQL
                     AMIAGFLTACPVNAWLIRSGIKERM"
BASE COUNT          459 a         1012 c         1023 g          404 t
ORIGIN      
        1 gccatcggcg ccgagtcgac cctggcgcgg atcgtccgtc tcgtcgagtc ggcccaggag
       61 gggaaggcac cgatccagcg tctggtcgat cgtgtcgccg cggtgttcgt tccggtcgtc
      121 atcggcattg ctctcctcac gctcctgagc tggggcctga cgaccggcaa ctggccgcag
      181 gcaatcctcg acgccgtcgc ggtgctcgtc atcgcctgcc cttgcgccct gggcctggcc
      241 acgcccacgg ccatcatggc gggcaccggc gtcgcggccc ggcacggcat cctgatcaag
      301 gatgccgaag ccctcgaggt ggcgcacgac ctgcggatcg tggcgttcga caagaccggg
      361 acgctcagcg agggcaggcc cgagctcgtg gcgttcgagg ccgccatcgg atcggccgac
      421 gcactgctgg cgacgagtgc ctccatccag gcgggcagcg agcacccgct ggcgcgggcc
      481 gtggtcgagg ccgccgatcg tgcgcggttg ccggtgccca tcgccacgga ggttcgcgca
      541 atcgccggac gcggcatgtc ggggcggatc ggccagcgcg agctgcgcct gggcagcggc
      601 ctcttcatgg gcgagctcgg tgtcgacacg tccgctctcg cagcgcgctc ggcggcgctg
      661 cagtccgaag ggcgcagcgt ctcctggctg gcagacgtca ccgccgagcc cgcgctggtg
      721 ggcctgctcg ccttcggcga ccggctgaag ccttcggcgg cgaacgcggt tcgcgcgctg
      781 caggcgctcg gcgtcagggc cgtcctgctc accggcgaca accggggcag cgccgcgctc
      841 gccgcgcagg cgctcggcat cgccgacgtt cgcgccgagg tgctgcccga ggacaaggcg
      901 cgcatcgtcg gcgagctgaa ggccggcggc gatcgcgtcg cgatggtcgg cgacggcatc
      961 aacgatgcgc cggccctcgc cgccgccgac gtcggcatcg ccatgtcgag cggaaccgac
     1021 gtggccatgt tcgccgccgg catcaccttg atgcgcggcg acccgtcgct cgttgtcgac
     1081 gcgatcgaca tctcgcggcg aacctacgcg aagatcaggc agaacctgtt ctgggcgttc
     1141 gcgtacaacg tcgtcggcat cccgctggcg gcgatgggcc tgctcagccc ggtcatcgcc
     1201 ggcgcggcga tggcgttgag cagcgtcagc gtggtgacca atgcgctcat gctgaggcga
     1261 tggaagggga gggcgcaatg agccggacag cgatcgatcc ggggtcgttc aacatcggcg
     1321 aggcagccgc gcgctcgggc gtgtcggcga agatggttcg tcactacgaa tcgctcggtc
     1381 tcctgccgaa ggtggctcgc accgagtccg ggtaccggca atacggcgag aaggaaatcc
     1441 acacgctgcg cttcgtccgg cgtgcccggg tcctcggctt cggcatggcc gagatcgcag
     1501 acctgctcaa gctctggcaa aaccggcgcc gcgccagcgc cgacgtgaag cgcatcgcgc
     1561 tcgcccacgt cgccgacctc gagcggcgca tggacgagat ggcggcgatg aagcgcaccc
     1621 tcgagcgact ggccgactgc tgccgcggcg acgaccgccc cgactgtccg atcctcgacg
     1681 aactcggcga gggttgacgc gggacacgcc acggacgatc gccggtccag ggtgcggccg
     1741 caccggcttc ttcctggaga ggaaatccgg acgggtgcgc aaaccgaagg tgaagcggga
     1801 cgactccggc gaagctcaca tcctttcctt gatgccgctt cggatgagcc acgcgttgac
     1861 ggggcaggcc gtcaggaagc cggcgatcat ggccagctgc atcgcgaacc agaactccgg
     1921 cgtatcgact tcggcctttg ccccgaaccc gtggtcgaac cagaggaact gggcgaacgc
     1981 catgaagccg tacatgccga tctgccacga gaccagcgac aaggtgtcgg ccttcagcgc
     2041 cgcgacgatg cccttgccag gcgacaggtt tcgcatcggc acgatggcga agtactggaa
     2101 gacgatgccc agcgcgaagg cgaagaggaa atcgagcacc cagtcggcga agatcttctc
     2161 gtcgaagagc gtatgccagc cgagccagac ggcgacgacc ggcgccgcga acgccagcca
     2221 ttcggcgatc acgtcgccga tcatgcagcc ggcaccgcag tgcagcgcgg ccttcccgac
     2281 ggtcgccgcg aacggctgag ccgctgtggg catcgcgcgc tggccggcgg ccgccttctg
     2341 cgtggccgct ccagtcgacg cgcgaccgta gcgaacgtag gcccatagcg tcaggaccgt
     2401 gccgaacaag gcggtcaccg gccagacgac gttcatgacc gccatcttct gggggcggcg
     2461 caccacgtcg atgccgaccg cgagcgcgca ggcgcctccg agcgcgagcg cgccgagact
     2521 cagcgcgtga aaccagggcg gaaacatcgg ggtgacaggg ccgaagcgaa gtgtcgatgg
     2581 gcgacaggcg ctgctcggca agccgcagac ccgcggcatg caggtcgatc gacgtgatcg
     2641 ccgggagact gccggcgccc ggcgtgcgcc gcggttgcac cgcgctggca caccagcaca
     2701 tcgcttcgtc gccgtccgcg aacgtgttcc ccctgcgcgc agcgacgtgc ggatgctcgg
     2761 cgatctaacc gtcggccacg accaactcgc cgctgcggcc ggccacccgc ccccagctca
     2821 gcgcccgccg gggcagcgcc tcgggatcga gcgtctcgaa gcgggtgcgc aggtcgacga
     2881 gctcatggcc ctggtcgc
//
LOCUS       DASRWS010000119         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2636466, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000119 DASRWS010000000
VERSION     DASRWS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2636466"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFF72_02120"
     CDS             complement(<1..364)
                     /locus_tag="VFF72_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZV91981.1"
                     /translation="MPFVQRYTRVAVVLHWLIALLIIVNVGLGLSAEALPDDWVRPVI
                     DTHKSIGITVLGLFLLRLLWRFSHPPPPLPSGLARWERIGAHLAHGALYFLMLALPVS
                     GWMHDSAWKDAATHPMSLF"
     gene            520..1995
                     /locus_tag="VFF72_02125"
     CDS             520..1995
                     /locus_tag="VFF72_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZV91982.1"
                     /translation="MIDPLAPALALSSSRLSPPPTPWRTVFIHAAVLVAWIALFSCAF
                     AQAGILAWAVGIAYIVYDTLLQLFVVVECRRLRAPVATPSIAVGSPSDLGVGVGVDVD
                     VGARLGAIVAAYNEATVLPATIAAMLGQTSPPDVIIVADDGSSDDTHAVLAAHYGLAP
                     PPLGSLGEASPVAPALRWLRLAHGGKARALNAALVECDAGIVLTVDADTLLEPGAVAA
                     VRAAFAAESELVAITGVITPRCKPTPGGRCLEWFQTYEYIRNFLSRYAWMQQGGLLLV
                     SGAFAGFRRSALLDVGGFDAACLVEDYELIHRLRRHAGDRGIDWRVRVLGDAQARTEA
                     PGSVGAFLRQRQRWFGGFLQTQYWYRSMVGDPKLGRLGTWMLPVKAIDTLQPLYGLTA
                     FALLVFYVATGRFAIVAPVAAVIGAKVAVDLGFHLWSVHLYRRWVGDAKRASLVAALV
                     AALAEPFTFQLLRHAGAALGWAAVVSRSRRWGRQTRFGLDR"
     gene            complement(2095..3429)
                     /locus_tag="VFF72_02130"
     CDS             complement(2095..3429)
                     /locus_tag="VFF72_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZV91983.1"
                     /translation="MVESQIPLPPGVSTLQVQTLRRLQRNSFDYFVHEVNDDNGLVLD
                     KTAENWPASIAAIGMALTVYAVGAERKFMSRERAVSRTLTTLRFLASLEQSEATDASG
                     YKGFFYHFLDMSSGRRAWNCELSSIDTALLMAGVLTAASYFDGTGTDEAEIRTLARTL
                     YERVDWTWMLAGQPTICHGWKPEQGFLTYHWEGYDEALVLYLLALGSPSHPIGSDSYA
                     AWCSTYEWKEVYGFEFLYAGPLFIHQMSHIWCDFRGIRDEFMRAHDCDYFENSRRATF
                     VQQQYAIRNPLGFKHVNDLCWGITASDGPGNQAKKVDGVERVFFDYIARGAPFGPDDG
                     TLAPWAVAASLPFAPEIVAPTISNLMALHVEVPNPYGFKASFNPSFHDGESKGKVGWV
                     SRYHFGINEGPTVLMIENERSGLVWSLMRRCEYLRKGLVAAGFTGGWLDAKA"
     gene            3616..>4847
                     /locus_tag="VFF72_02135"
     CDS             3616..>4847
                     /locus_tag="VFF72_02135"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZV91984.1"
                     /translation="MASQARAASRALAASPVAARNAALRSLASSLRAAVGPLAGANRR
                     DLDAGLAAGLSPPLVERLRLGRDAIETVALGCEQIAAMADPIGAVSGLVEQPSGIRVG
                     RMRVPIGVFAMIYESRPNVTIEAASLAIKSGNACILRGGSEALHSNLALAALVRDALA
                     GAGLPPDAVQLVGTTDRAAVGHLIAAEGAVDLVIPRGGKGLIERIAREAKVPVLKHLD
                     GNCHVYVDAAADLAMALAIVDNAKTQKLSPCNAAESLLVHADVAASFLPAIGAVFAAK
                     GIEMRCDPQALALLGGVAGAKLVPAAEADWSEEYLAAIISIKVVASLDAAMSHIDRYG
                     SHHTDAIVTADESAAKKFLTEVDSAIVLHNASTQFADGGEFGFGAEIGIATGRMHARG
                     PVGVEQLTTFKYRIRGTG"
BASE COUNT          706 a         1684 c         1661 g          796 t
ORIGIN      
        1 cgaagagcga catcgggtgc gtggcggcgt ccttccaggc cgagtcgtgc atccagcccg
       61 acaccggcag cgccagcatc agaaagtaga gggcgccatg cgccaggtgc gcgccgatcc
      121 gctcccagcg cgccaggccg ctcggcaacg gcggcggcgg gtgcgagaag cgccacagga
      181 gccggagcag gaaaagaccg agcacggtga tgccgatcga cttgtgcgtg tcgatgaccg
      241 ggcgcaccca gtcgtcgggc aatgcctcgg ccgacagccc gaggccgacg ttgacgatga
      301 tgagcaacgc gatgagccag tgcaggacca cggcgacccg cgtgtagcgc tgaacgaacg
      361 gcatggatgg catttcacct catcccggca gttgccgtgc acttcggtgc gatgtccgcg
      421 aacacgacac gccgacgcct ccgggaaagg gcgaccccgt gcctgcgggc aaatcgtttc
      481 cccgataggc tcgcgccttt gtccggaacg cggccgccct tgatcgatcc cctcgcgccg
      541 gcgcttgcgc tgtcgtcgtc ccgcctttcg ccgccgccga cgccctggcg gaccgtcttc
      601 atccacgcgg ccgttctggt cgcatggatc gccctgttct cgtgtgcctt cgcgcaggcc
      661 gggatcctgg cctgggcggt gggcatcgcc tacatcgtct acgacaccct gctgcagctg
      721 ttcgtcgtcg tcgagtgtcg gcgtctgcgg gctccggtgg cgacgccttc catcgcggtc
      781 ggctcgccat cggatctcgg cgtcggcgtc ggcgtcgacg tcgacgtcgg cgcgaggctc
      841 ggcgccatcg tcgctgccta caacgaggcc acggtgcttc cggcgacgat cgcggccatg
      901 ctcgggcaga cgagcccgcc cgacgtgatc atcgtcgccg acgacggctc gagcgacgac
      961 acccacgccg tgctggcggc gcactacggc ctcgccccgc cgccgctcgg ctcgctcggc
     1021 gaagccagcc cggtcgcgcc ggcgctgcgc tggcttcgcc tcgcgcatgg cggcaaggcc
     1081 cgggcgctca acgcggctct cgtcgaatgc gacgccggga tcgtcctcac cgtcgatgcc
     1141 gacaccttgc tcgaacccgg ggcggtcgcg gccgtccgtg ccgcgttcgc ggccgagtcg
     1201 gagctcgtcg cgatcaccgg cgtgatcacg ccgaggtgca agccgacgcc gggcggccgc
     1261 tgcctggaat ggttccagac ctatgagtac atccgcaact tcctgtcgcg ctacgcctgg
     1321 atgcagcagg gcggcctgct cctggtctcg ggcgccttcg ccggcttcag gcgcagcgcg
     1381 ctgctcgatg tcggcggctt cgacgcggcg tgtctcgtcg aggactacga gctgatccat
     1441 cggctgcgcc ggcacgcggg cgatcgcggc atcgactggc gggtccgcgt cctgggcgac
     1501 gcgcaggcgc gcaccgaggc gccgggcagc gtcggcgcct tcctgcgcca gcgccagcgc
     1561 tggttcggcg gcttcctgca gacgcaatac tggtaccgct cgatggtggg cgacccgaag
     1621 ctcggccggc tcggcacctg gatgcttccg gtcaaggcca tcgataccct gcaaccgctt
     1681 tacgggttga cggcgttcgc gctgctcgtc ttctacgtcg cgacgggtcg cttcgcgatc
     1741 gtcgcgccgg tggcggccgt catcggtgcc aaggtggcgg tcgacctcgg ctttcatctc
     1801 tggtcggtcc atctctaccg gcgctgggtc ggcgacgcga agcgcgccag cctcgtcgcc
     1861 gcgctcgtcg cggccctggc cgagccgttc acgttccagc ttcttcgtca tgccggcgct
     1921 gcgctcggct gggccgccgt cgtcagtcgc agccggcgct ggggacgaca gacgcgcttc
     1981 gggctcgatc gctgacccgt gcgtcgtttc tgccgggtct tcgacgatcg gtcgaagacc
     2041 gctcgtggcg ttgcaatgcg ccgagagcct ttcgcctctg gcgcgagcgc gaattcacgc
     2101 cttcgcgtcg agccagccgc cggtgaagcc ggccgcgacc aggccctttc tcaggtactc
     2161 gcagcgccgc atcagtgacc acaccaggcc gctccgctcg ttctcgatca tcagcacggt
     2221 cgggccttcg ttgatcccga agtgatagcg cgacacccag ccgaccttgc ccttcgactc
     2281 gccgtcgtgg aacgacggat tgaacgatgc cttgaagccg tacgggttgg gcacctcgac
     2341 gtgcagggcc atcaggttcg agatcgtcgg cgcgacgatc tcgggcgcga acggcagcga
     2401 tgcggcgacg gcccacgggg cgagggtgcc gtcgtcgggc ccgaagggcg cgccgcgggc
     2461 gatgtagtcg aagaagacgc gctcgacgcc gtcgaccttc ttggcctggt tgccgggccc
     2521 gtcgctcgcg gtgatgcccc agcacaggtc gttgacgtgc ttgaagccga gcgggttgcg
     2581 gatcgcgtac tgctgctgca cgaaggtggc gcggcggctg ttctcgaaat agtcgcagtc
     2641 gtgagctcgc atgaactcgt cgcggatgcc gcgaaagtcg caccagatgt gcgacatctg
     2701 gtggatgaag agcggaccgg cgtagaggaa ctcgaaaccg tagacctcct tccactcgta
     2761 ggtgctgcac catgcggcgt agctgtcgct gccgatcggg tggctgggcg agccgagggc
     2821 gagcaggtag aggacgaggg cttcgtcgta gccctcccag tggtaggtca ggaatccctg
     2881 ctcgggcttc cagccgtggc agatcgtcgg ctggccggcc agcatccagg tccagtcgac
     2941 ccgttcgtag agcgtgcggg ccagcgtccg gatctcggcc tcgtccgtgc cggtgccatc
     3001 gaagtagctc gccgccgtca gcacgcccgc catcagcaag gcggtatcga tgctcgacag
     3061 ctcgcagttc catgcgcgcc ggcccgagct catgtcgagg aagtggtaga aaaagccctt
     3121 gtagccgctc gcgtccgtgg cttcgctttg ctcgagcgaa gcgagaaacc gcagcgtcgt
     3181 cagcgtccgc gagacggccc gttcgcgcga catgaacttc cgctcggcgc cgaccgcgta
     3241 gaccgtcagc gccatgccaa tggcggcaat gctggcgggc cagttctcgg cggtcttgtc
     3301 gagtacgagg ccgttgtcgt cgttgacctc gtggacgaag tagtcgaagg aattgcgctg
     3361 cagccggcgc agagtctgca cctgcagggt cgatacgccc ggcggcaggg gaatttgcga
     3421 ttcgaccatt cttgtcagtc agtctggaag tcggacaggc cgacgccgcg aaaaacgggc
     3481 gccctgcacg ggttcgagcg tagcgcaggc gcgccgttcc cgagtccggt gcctgcttgt
     3541 tccgagcccg gtcgcgccga ctctgaaaca atccctgccg tgacctcacc agaccttcag
     3601 gcgaccatcg cgctcatggc ctcgcaggct cgggccgcca gccgggccct ggccgcttcg
     3661 ccggtcgccg cccgcaacgc cgcgctgcgg tcgctggcaa gttcgctgcg tgcggccgtc
     3721 gggccgctgg ccggggcgaa tcggcgcgat ctcgatgcgg gcctggccgc cggcctctcg
     3781 ccacccctcg tcgagcggct gcgcctcggc cgcgacgcca tcgagacggt ggcgctgggt
     3841 tgcgagcaga tcgccgccat ggccgatccg atcggtgcgg tgagcggcct cgtcgagcag
     3901 ccgagcggca ttcgcgtcgg ccgcatgcgc gtgccgatcg gcgtcttcgc catgatctac
     3961 gaaagccgtc cgaacgtgac catcgaggcg gcgtcgctgg cgatcaagag cggcaacgcc
     4021 tgcatcctgc gcggcggctc ggaagcgctt cattccaacc tggccctcgc cgcgctggta
     4081 cgggacgcac tcgccggggc cgggctgccg cccgatgccg tgcaactcgt cggcacgacc
     4141 gaccgggccg cggtcggcca tctgatcgcc gccgaaggcg cagtcgacct cgtcattccg
     4201 cgtggcggca agggcctgat cgagcggatc gcccgcgagg cgaaggtgcc cgtcctcaag
     4261 cacctcgacg gcaactgtca cgtctacgtc gacgccgccg ccgacctggc gatggcgctg
     4321 gcgatcgtcg acaacgccaa gacgcaaaag ctcagcccct gcaacgccgc cgaatcgctg
     4381 ctcgtccacg ccgatgtcgc ggcaagcttt ctgccggcga tcggtgccgt gttcgccgcc
     4441 aagggcatcg agatgcgctg cgatccgcag gcgctggcgc tcctcggcgg cgtcgccggc
     4501 gcgaagctgg tgccggcggc cgaagccgat tggagcgagg agtacctcgc cgcgatcatc
     4561 agcatcaagg tcgtcgcatc gctcgacgcg gcgatgtcgc acatcgaccg ctacggctcg
     4621 catcacaccg acgcgatcgt gacggcggac gaaagcgcgg cgaagaagtt tctcaccgaa
     4681 gtcgactcgg ccatcgtgct gcacaacgcc tcgacgcagt tcgccgatgg cggcgagttc
     4741 ggtttcggcg ccgagatcgg catcgcgacg ggccgcatgc acgcacgcgg gccggtcggc
     4801 gtcgagcagt tgaccacgtt caaatatcgc attcgcggaa cgggaca
//
LOCUS       DASRWS010000120         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3493970, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000120 DASRWS010000000
VERSION     DASRWS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493970"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFF72_02140"
     CDS             complement(<1..473)
                     /locus_tag="VFF72_02140"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZV91985.1"
                     /translation="MPPRKPLPDTGRAAAARPTRHGRDPERTRARLLAAARREFMRHG
                     FSGARVERIARSARSSERMLYYYFGSKEKLYLAVLEGVYADLGAAESALVLDASRPVE
                     ALTRLVEFTFGYYLAHPEFVALLSNENLMRGRHITKSVKVKRLSRPLLDVLETILV"
     gene            608..1447
                     /locus_tag="VFF72_02145"
     CDS             608..1447
                     /locus_tag="VFF72_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZV91986.1"
                     /translation="MIPRFERPASAAEAAHLLSAGEWTVLAGGTDLYPAHVGRPLGRP
                     LLDLTRIAALRGIRRGDNGWTIGATTTWTDVVRADLPPLFDALKAAGREIGGVQIQNV
                     GTVAGNVCNASPAADGTVALLALGAEVELLSARGTRRVDLAGFVLGSRKTARRDDELV
                     VALHVPARGAGARSVFRKLGGRRHLVISISMVGVAVDVDAAGRATHAAVAVGSCSAVA
                     QRLPTLEARLVGLDAAGWADAVGAEDLAALTPIDDVRGSADYRRDATLTLLGRALSEL
                     AAA"
     gene            1444..>2193
                     /locus_tag="VFF72_02150"
     CDS             1444..>2193
                     /locus_tag="VFF72_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV91987.1"
                     /translation="MTARDLEAAQIVHLSVNGTPCELVGEPARRLSDALRDELGLTGT
                     KIGCHAGDCGACTVLLDSAQVCACIVAVGQCEGRSVTTVEGLAGPDGELSLLQRAFVA
                     HGAAQCGICTPGMLMSAESLLREVARPSEAQVRDALAGVLCRCTGYTKIVEAVLAAAT
                     GVVAPARPPAAGCSVGERVERLDAPVKVRGEERFGADQRPAVANEAKADALAMRVVRS
                     PYPHAAFDVDVVDAFRQRWPGVVEVLVAADVP"
BASE COUNT          281 a          798 c          798 g          316 t
ORIGIN      
        1 acgagtattg tctccaggac gtcgagcagc ggccgcgaca gccgcttgac cttcaccgac
       61 ttggtgatat gccgcccgcg catcaggttc tcgttcgaca gcaaggcgac gaattcgggg
      121 tgcgcgaggt aatagccgaa cgtgaactcg accagccggg tcagcgcctc gaccgggcgg
      181 ctggcgtcga gcaccagcgc gctctcggcg gcgccgaggt cggcatagac gccttcgagc
      241 acggcgaggt agagcttttc cttgctgccg aagtagtagt agagcatccg ctcgctcgag
      301 cgtgccgacc tggcgatgcg ctcgacacgg gcgccactga agccgtgccg catgaactcg
      361 cgccgcgccg cggcgagcag gcgcgcgcgg gtccgttccg ggtcgcggcc gtgccttgtc
      421 gggcgggcgg ccgcggcgcg gccggtgtcg ggcaggggct ttcgaggagg catgtcgacg
      481 gcggtggtcg tgccgccagg ctttccgccc gcgccatcgc gcggcgcggt ctctagccaa
      541 ggcagcggcg gcatgctaga ttggctcgcc aatgaagtaa acacttcatt ggcagggagc
      601 ttatgccgtg attccccgat tcgagcgccc cgcgtcggcg gccgaggccg cgcacctgct
      661 ttcggctggc gagtggaccg tgctcgccgg cggcaccgac ctctatccgg cgcatgtcgg
      721 tcggccgctc ggccggccgc tcctcgacct cacccgcatc gccgcgctgc gcggcatccg
      781 gcgcggcgac aacggctgga ccatcggcgc gacgacgacc tggaccgatg tcgtccgcgc
      841 cgacctgcca ccgctcttcg acgccctcaa ggcggccggt cgcgagatcg gcggcgtgca
      901 gatccagaac gtcggcacgg tggccggcaa cgtctgcaat gcgtcgccgg ccgccgatgg
      961 cacggtcgcc ttgctcgcgc tcggcgccga ggtcgagttg ctcagcgcac gcggcacgcg
     1021 gcgcgtcgat ctcgccgggt tcgttctcgg cagccggaag accgcccgcc gcgacgacga
     1081 gctcgtcgtc gccctgcacg tgccggcgcg cggagcgggc gcgcgctcgg tctttcgcaa
     1141 gctcggcggc aggcgtcacc tcgtcatctc gatcagcatg gtcggcgtcg ccgtcgacgt
     1201 cgacgcggca ggccgcgcca cccacgccgc agtcgcggtc ggcagctgct cggccgtcgc
     1261 gcagcggctg ccgacgctcg aggcgcgcct ggtcggcctc gacgccgccg gctgggccga
     1321 tgcagtcggc gccgaagacc tcgccgccct gacgccgatc gacgacgtcc ggggcagcgc
     1381 cgactaccgc cgcgacgcga cgctgacgct gcttggccgc gctctttcgg agctcgccgc
     1441 cgcatgaccg cgcgcgacct cgaggcggcg cagatcgttc acctgagcgt caacgggacg
     1501 ccgtgcgagc tggtcggcga gccggcgcga cgtctctccg acgcgctgcg cgacgagctc
     1561 ggcctcaccg gcacgaagat cggctgccac gccggcgact gcggcgcctg cacggtgctg
     1621 ctcgattccg cgcaggtctg cgcctgcatc gtcgccgtcg gccagtgcga gggccgatcg
     1681 gtcacgacgg tcgaaggcct ggccggcccc gacggcgagc tgtcgctgct gcaacgcgcc
     1741 ttcgtcgccc atggcgcggc gcaatgcggc atctgcacgc ccggcatgct gatgagcgcc
     1801 gaatcgctgc tgcgcgaggt ggccaggccg agcgaggcgc aagtgcgcga cgcgctggcc
     1861 ggtgttctct gccgctgcac cggctacacc aagatcgtcg aggcggtgct cgccgcggcc
     1921 accggcgtcg tcgcgccggc gcggccgccg gcggcgggct gttcggtcgg cgagcgggtc
     1981 gagcgactcg acgcgcccgt caaggtgcgc ggcgaggagc gcttcggcgc cgaccagcgc
     2041 ccggccgtcg cgaacgaagc gaaggccgac gcgctggcga tgcgtgtggt ccggtcgccg
     2101 tatccgcacg ccgcgttcga cgtcgacgtc gtcgacgcct tcaggcagcg ctggcccggc
     2161 gtcgtcgaag tgctcgtcgc agccgacgtg cca
//
LOCUS       DASRWS010000121         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1488868, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000121 DASRWS010000000
VERSION     DASRWS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488868"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFF72_02155"
     CDS             complement(<1..1284)
                     /locus_tag="VFF72_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HZV91988.1"
                     /translation="MSPHLRYRRLGWAIALAAFCGVALGAAPHDFSMLGKYCSKCHNT
                     EDWAGGIAFETLSPENIPEDAETWEKAMRKLRGGLMPPAGNPQPDRKSVEAFVDWVEG
                     TIDRTALPHVEPGRVALHRLNRKEYANAVRDLLDVDVDARELLPRDDSHDGFDNIASA
                     LQVSPSFLEQYLAAARKVAKEALGDPQSRPVGTTYTAGGNGNQFFRAEGLPLGTRGGI
                     VVEHEFPADGEYVINIANMAQAIWVYNMEFENQVVVTLDRRKIYETSVGGEADMKAID
                     QRQDPAVDAINKRLKNIRFTATAGPHQIGVTFRQRSYAESESWLESNVPGGGQERVLR
                     VSSFEVRGPLSVTGVSETPSRRRIFSCHPTAASEEPACAREIVQRLAAQAFRHPVSDA
                     DIADLMRFYEAGRLEGGFESGIRQAITAMLADPEFL"
     gene            1466..>2333
                     /locus_tag="VFF72_02160"
     CDS             1466..>2333
                     /locus_tag="VFF72_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91989.1"
                     /translation="MPQTTQSSELALAEEHDAAGRHNEAIDVLARATHSGNVACMRQL
                     GKRLLAGDRAPLLAAEGARFLLDAAQRGDGEAAARIAALCALGLYLKPSWSDALRWLV
                     VAAQRGWQPAQGQLRALAETPAGSAEVPDWSRLASRLELTPWQSSPPARELSVDPQVQ
                     AFDSFIGDAVCEWVIARARGRLTRALVYNAVNQEDYASSTRTNSVANISLADIELLDV
                     LLQVKMSYACRVPMSHMEAPAVLHYCVGEEAANHYDFVNPNTPDYAAEIARNGQRIVT
                     FLIYLNDDYDGGE"
BASE COUNT          405 a          759 c          745 g          424 t
ORIGIN      
        1 caggaactcg ggatccgcca gcatggccgt gatggcttgc cggatccccg attcgaatcc
       61 cccctccagc cggcccgcct cgtaaaaccg catcagatcc gcgatatcgg catcgctgac
      121 gggatgccga aaggcctgcg cggcgaggcg ctgaacgatc tcacgcgcgc aagcgggctc
      181 ttccgaggca gcagtcgggt ggcagctgaa gattcgtcga cggctcggtg tctcgctgac
      241 tcccgtgacg ctcaaagggc cacgcacttc gaacgagctt acgcgcagca cgcgctcctg
      301 gccgccaccg gggacgttgg attcgagcca gctttcggac tccgcgtaac tgcgctgccg
      361 gaatgtgacg ccaatctggt gagggcctgc ggtcgcggtg aaccgaatgt tcttcaggcg
      421 tttgttgatc gcatcgacgg cagggtcctg ccgctggtcg atggccttca tgtcggcttc
      481 gccgccgacc gaggtctcgt agatcttgcg gcgatcgagc gtcacgacga cctggttctc
      541 gaactccatg ttgtagaccc agatcgcctg cgccatgttg gcgatgttga tgacgtactc
      601 gccgtcggcc gggaactcat gctcgacgac gatgccgcca cgcgtaccga gcggcaggcc
      661 ttcggcgcgg aagaactggt tgccgttacc tcccgcggtg tacgtcgttc cgacggggcg
      721 ggattgcggg tctcccagag cttccttcgc taccttgcgt gccgctgcga gatactgctc
      781 cagaaatgac ggcgacacct gcagcgcgct ggcaatgttg tcgaagccgt cgtgggagtc
      841 gtcgcgaggt aacagctcgc gcgcatcgac gtcgacatcg agcaagtcac gaaccgcgtt
      901 cgcatactcc ttgcgattga ggcgatgtag cgcgactcgg cccggctcaa cgtggggcag
      961 cgcggtgcga tcgatagtgc cttccaccca gtcgacgaac gcttcgacgg atttgcggtc
     1021 gggctgcgga ttgccggcgg gcggcatcag gccccctcgc agcttccgca tggccttttc
     1081 ccaggtttct gcgtcctcgg gaatgttctc cggcgacagg gtctcgaagg cgattccgcc
     1141 tgcccagtcc tccgtgttgt ggcacttgct gcagtacttc ccgagcatcg aaaagtcgtg
     1201 cggtgcggcg cccagggcaa cgccgcagaa ggcggcaagc gcgatggccc agcccaatcg
     1261 tcggtagcgc aggtgtggac tcatcgtcgg tcgagtgctc tgcaacgcag ctaagatcca
     1321 caaagataag aggaaaacag taatccccgc cagtgccgcc acgtttctta tgccaaatcg
     1381 agccgctctc ggaaccaccc cgatgggggt gccggctgcc gcatccgttg tgcggacgcc
     1441 tatttcgctc ttaagcttcg gaaacatgcc gcagaccact cagagttccg aactggcgct
     1501 cgctgaagag cacgacgccg ccggccgaca caacgaggcg atcgatgtcc tggcacgcgc
     1561 cacccattcg ggaaacgtgg cctgcatgcg gcagcttggg aaacggctgc tcgctggcga
     1621 ccgggcgccc ttgctcgctg ccgagggcgc ccgcttcctg ctggatgcgg ctcagcgagg
     1681 cgacggcgaa gcggccgcgc ggattgcggc gctgtgcgcc ctcggcctgt acctcaagcc
     1741 gagctggtcg gatgcgctgc gctggcttgt cgtcgcggcc cagcgagggt ggcagcccgc
     1801 acaggggcaa ctgcgggcgc ttgccgaaac gccagcgggc tccgccgaag tgcccgactg
     1861 gtcgcggctg gcgagccgac tcgagctcac cccttggcag tcgagcccgc ccgctcgcga
     1921 gctgagtgtg gatcctcagg tccaggcgtt cgattcgttc atcggcgacg ccgtatgcga
     1981 gtgggtcatc gcacgagcgc gtggccgctt gactcgagct ctggtctaca acgccgtgaa
     2041 tcaagaagac tacgccagct cgacgcgcac caattcagtc gcgaacatca gcctggcaga
     2101 catcgagctg ctggacgtgc tgctgcaggt gaagatgtcc tatgcctgcc gcgtgccgat
     2161 gagccacatg gaggcgcctg cggtcctgca ctactgcgtc ggcgaggaag ccgcaaacca
     2221 ttacgacttc gtcaatccca acacaccgga ctacgccgcg gagatcgccc gcaacggtca
     2281 gcgcatcgtg acgtttctca tctatctcaa cgatgactac gacggtggcg aaa
//
LOCUS       DASRWS010000122         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1489502, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000122 DASRWS010000000
VERSION     DASRWS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489502"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFF72_02165"
     CDS             complement(<1..191)
                     /locus_tag="VFF72_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV91990.1"
                     /translation="MAVFALAAPLAAPAQGAVADPHVASIEQLLGRLELANTSYEHGP
                     GRIDWRGTVASHTDCSGLI"
     gene            complement(250..1428)
                     /gene="metK"
                     /locus_tag="VFF72_02170"
     CDS             complement(250..1428)
                     /gene="metK"
                     /locus_tag="VFF72_02170"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZV91991.1"
                     /translation="MSNDFLFTSESVSEGHPDKVADQVSDAILDAIFAQDPRSRVAAE
                     TLCNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSN
                     DIAQGVDHASDDHLNTGAGDQGLMFGYACDETPDLMPAPIYYAHRLVERQAQLRKDGR
                     MPYLRPDAKSQVTMRYVDGKPHSIDTVVLSSQHAPEMSLGTKMKPEFYEAVIEQIIKP
                     VLPREWLKDTKYLVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPS
                     KVDRSAAYAARYVAKNIVAAGIARQCQIQVAYAIGVARPMNVTIYTEGTGKISDEKIA
                     ALVSEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGRDEPEFSWERTDKAQMLRAAAGL
                     "
     gene            1672..2226
                     /locus_tag="VFF72_02175"
     CDS             1672..2226
                     /locus_tag="VFF72_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZV91992.1"
                     /translation="MGDITELIHQAHEGDPTALDRLFAAIYPDLRRIARARLRGRLAE
                     ADLDTTALVNECFLKMHAARRLDSPDRGRFFAYMAMAMRSIIVDIARARASERRGGGA
                     AHVELDADNEPAGDLHDAAQIIRVHEALDEISHLDERLSRLVEMRYFGGYEDEEIAQA
                     LDLSVRTVRRDWQKARLLLSAALR"
     gene            complement(2249..>2607)
                     /locus_tag="VFF72_02180"
     CDS             complement(2249..>2607)
                     /locus_tag="VFF72_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV91993.1"
                     /translation="WSGSERDQSDGRLALLRSRQGRHDEAVGLARSSRDRLRTQPSKI
                     VRALAESTLGQVLFAAGRPGDAAAPLREAQRLFAERQTIRTPDRIDVDNALVAAGGAA
                     ATSAAAVPAPPRQTTR"
BASE COUNT          361 a          861 c          928 g          457 t
ORIGIN      
        1 tcgatgagac ccgagcagtc ggtgtgcgag gcgaccgtgc cgcgccagtc gatgcggccc
       61 ggtccgtgct cgtaactcgt gttcgcgagt tcgagtcgtc cgagcagctg ctcgatcgaa
      121 gcgacgtgcg ggtcggcgac ggcgccttgg gccggcgcgg ccagcggcgc cgcgagagcg
      181 aagacggcca cgagggcgct tgccgcacta gaccgaaagg ccggcccgcc catcgccttc
      241 ccgattcgtc tagagccccg ccgccgcgcg cagcatctgc gccttgtcgg tgcgttccca
      301 gctgaactcg ggctcgtcac ggccgaagtg gccgtaggcg gcggtcttct cgtagatcgg
      361 gcgcagcagg tcgagcatct ggatgatgcc cttggggcgc aggtcgaaat gctcgctgac
      421 gagcgccgcg atcttctcgt cggagatctt tccggtgcct tcggtgtaga tcgtcacgtt
      481 catcggcctg gcgacgccga tcgcgtaggc cacctggatc tggcactgcc tggcgatgcc
      541 ggcggcgacg atgttcttgg cgacgtagcg cgccgcgtag gctgccgagc ggtcgacctt
      601 gctcgggtcc ttgcccgaga acgcgccgcc gccgtgcggg caggcgccgc cgtaggtgtc
      661 gacgatgatc ttgcggccgg tcaggccgca gtcgccctgc ggtccgccga cgacgaagcg
      721 gccggtcgga ttgacgaggt acttggtgtc cttcagccac tccctgggca gcaccggctt
      781 gatgatctgc tcgatcaccg cctcgtagaa ctcgggcttc atcttcgtgc ccaggctcat
      841 ctcgggcgca tgctggctcg acagcaccac ggtgtcgatc gaatgcggct tgccgtcgac
      901 gtagcgcatc gtcacctggc tcttcgcgtc ggggcgcagg tagggcatgc ggccgtcctt
      961 ccgcagctgc gcctggcgct cgacgagtcg gtgcgcgtag tagatcggcg ccggcatcag
     1021 gtcgggggtc tcgtcgcagg cgtagccgaa catcaggccc tggtcgccgg cgccggtgtt
     1081 gaggtggtcg tcgctcgcgt ggtcgacgcc ctgggcgatg tcgttgctct gcttgtcgta
     1141 ggcgacgagc acggcgcagc ccttgtagtc gatgccgtag tcggtgttgt cgtagccgat
     1201 cctcttgatc gtgtcgcgcg cgacctggat gtagtcgacg tgcgcgttgg tcgtgatctc
     1261 gccggccagc accacgaggc cggtgttgca cagcgtctcg gcggcgaccc gtgagcgcgg
     1321 gtcctgggcg aagatggcgt cgaggatcgc gtcggagacc tggtcggcga ccttgtcggg
     1381 gtggccttcg gagaccgatt cggaggtgaa gaggaaatcg ttcgacacgc tgtgtctcca
     1441 gtgctggaag tcgtcgggcc cgcgatgaac gtcgcgttgc cggcctgggc tctggaggag
     1501 cggcgaacgc tttagcggaa ctcttttcgc gtcgccccgc aagttgttca ataactcggc
     1561 gaccgggcca ttataggaag agtcggcgcc ggctggctcg agtggccggg ctgcgtggcc
     1621 gccgcgaagt ccggcatgcc atcatccccg tcactcgtac cgacgacttc catgggcgac
     1681 atcaccgagc tcatccacca ggcccacgag ggcgacccga ccgcgctgga tcgcctgttc
     1741 gcggcgatct accccgacct gcggcggatt gcccgggcgc gcctgcgcgg ccggctggcc
     1801 gaggccgacc tcgacacgac cgccctcgtc aacgaatgct tcctgaaaat gcacgcggcg
     1861 cggcgcctcg actctcccga ccgggggcgc ttcttcgcct atatggcgat ggcgatgcgt
     1921 tcgatcatcg tcgacatcgc gcgggcccgg gccagcgagc ggcgcggtgg cggcgccgcc
     1981 cacgtcgagc tcgatgccga caacgagccg gcaggcgatc tgcacgatgc ggcccagatc
     2041 attcgcgtcc acgaggcgct cgacgagatc tcgcatctcg acgagcgact gtcgcggctc
     2101 gtcgagatgc gctacttcgg cggctacgag gacgaggaga tcgcacaggc gctcgacctg
     2161 tcggtgcgca ctgtgcggcg cgactggcag aaggcgcgcc tgttgctaag cgcggcattg
     2221 cgctgacagc ctcctgtcgc acgggctgtc aacgagtggt ctggcgtggc ggtgccggca
     2281 ccgccgccgc ggaagttgcc gcagcgcctc ctgcggccac cagcgcgttg tcgacgtcga
     2341 tccgatcggg cgtgcggatc gtctgccgtt cggcgaagag gcgctgcgct tcgcgcagcg
     2401 gcgccgcggc gtcgccgggg cgaccggccg cgaacaggac ctggccaaga gttgactcgg
     2461 cgagcgcgcg aacgatcttt gatggctgcg ttcgcagccg gtcgcgcgag gagcgggcca
     2521 acccgacggc ttcgtcatgg cgcccctggc ggctgcgcaa cagggcgagc cgcccgtcgc
     2581 tctggtctct ttcggagccg gaccaag
//
LOCUS       DASRWS010000123         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3791067, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000123 DASRWS010000000
VERSION     DASRWS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3791067"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="cobA"
                     /locus_tag="VFF72_02185"
     CDS             complement(<1..838)
                     /gene="cobA"
                     /locus_tag="VFF72_02185"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZV91994.1"
                     /translation="MTSSAPGRVAFVGAGPGQADLITLRGAARLREADVVLFDSLADP
                     ALRALAPRARWIDVGKRGFRGVAHGPADGGENTGQATINALLVKHAASSRLVVRLKGG
                     DPSVFGRLEEELEALDAAGIACEVVPGVTAALAAAAATQRPLTRRGQGRSVALTTAMT
                     HDGGLGAGRHADTEVFYMAGKQLGALARRLGDAGWSTATAALVVSRAGWPDELASDHT
                     IADLACAALLHAGRPTVVTVGVGARPVGGKRRPAADASELPFLRPELEGQDVSSGPAR
                     PRP"
     gene            complement(835..1332)
                     /locus_tag="VFF72_02190"
     CDS             complement(835..1332)
                     /locus_tag="VFF72_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZV91995.1"
                     /translation="MTTAAMQFIVAEDDASPRADGNVAVEAGTTALIDWSEWLRLRAD
                     WPADLRASVALPNDLDIDETLADLGRFGRIALRFPKWTDGRAYSQARLLRVRHRFTGE
                     LRAMGDVIADMAAQLHRTGFDAAVLRPGESVEVAQRMLGLIRVFYQGDAHERRLRFAR
                     VEATA"
     gene            complement(1329..3041)
                     /locus_tag="VFF72_02195"
     CDS             complement(1329..3041)
                     /locus_tag="VFF72_02195"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZV91996.1"
                     /translation="MYRYSEFDHQFVRQRAAQFRDQLTRHLAGTLSADDFGHMRRQNG
                     WYVQRHAPMARVAIPYGTLSSTQMRALARIARNHDRGYGHFTTRTNIQFNWIPLERAA
                     DVMDELAEVGMHGIQTCGNCIRNITADAFAGIARDEVADPRPFCEIMRQWSSLHPEFA
                     ALPRKFKIAVNGALEDRAATAWYDVGLQLRRNEAGELGFQVRVGGGMGRTPMIGSVIR
                     EFLPWPQLLAYLEAVLRVYNEFGRRDNIWKARIKVIVKAFGAEFTHKVEEEFAAILGN
                     DGDGVAPLISEAELARVAAHFETPKGVADQAASTSVVASLDESPAYRRWRQRNVHPHR
                     LAGHCAVTLSLKRPGQAPGDTTDRQMDAAAELADHFSAGELRVTHQQNLVLPWVREAD
                     LHRLWLEARESSFATPNVGLLTDMICCPGGDVCSLANARSIPVSAHIAERFDDLDELH
                     DIGDISLNISGCINSCGHHHSGHIGVLGVDKDGEEWYQVTLGGTDGAGGHGPAAPGRV
                     LGPAFRDSEIPDVIESLIETYLARRQGAGERFIDTFRRLGVEPFRAAANRARRDVAEP
                     ALVE"
     gene            3194..4162
                     /locus_tag="VFF72_02200"
     CDS             3194..4162
                     /locus_tag="VFF72_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZV91997.1"
                     /translation="MNKKPPLISPARRRGLLAGAGGTASLAMAALAGCAGVAPSPPVP
                     ALPPAPPPPAAPAAPAPPPRRLRVGLALGGGAARGFAHIGVIQALEQGGVEIDLVAGT
                     SAGSLVAALYASGRNGHDLELLADAMDETAFADWSYPGRGLIRGEALAKFVRDKTGGR
                     LIEQMRLPLGIAATDLESGEAVLFRRGDTGTAVRASSAVPAVFQPVKIGSREYVDGGL
                     SAPVPVHHAIAMGAEFVIAVDISAVTEGNATGDALRMLLQTFTIMGSHIKAFELKDAD
                     LVLQPKLAGVSGADFAARRHTIAAGRDVTLAHLAQLRGLLAAKSRL"
     gene            complement(4268..4819)
                     /locus_tag="VFF72_02205"
     CDS             complement(4268..4819)
                     /locus_tag="VFF72_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZV91998.1"
                     /translation="MMTVEQIMSAHKANVETLFGLSGLAFEGVEKIIELNIQATRTAL
                     TEAAETTQAALSVKDAQELLALQASLMQPTAEKAAAYGRHVYEIAATTGAEVNRVAEA
                     TAADAQAKFMAVVDNAVRNAPAGTENAVALVKSAVAAANNAFESVQKASKQAAEVADA
                     NFQAMSSTAVRATQAATKSRKAA"
     gene            5018..5944
                     /locus_tag="VFF72_02210"
     CDS             5018..5944
                     /locus_tag="VFF72_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZV91999.1"
                     /translation="MKAFHSDGHPLPLPAGHRFPQSKYRLLRTLVEGRPDLDLVEAPA
                     ASDDLLALAHSPAYVRSVASDGLTPLEQRAIGFPWSEAMVERSRRSVGATCEAARSAL
                     REGVAANLAGGTHHASADRGSGYCVFNDVAVASRLMQAQASAAPLRIVVIDLDVHQGD
                     GTARIFEGDPSVFTVSLHGERNFPFRKATSDLDVALPDACGDADYLASLDAALGVVLA
                     EHARAAFGLAFYLAGADPHEDDRLGRLKLSAAGLLERDRRVLGLLREQRIPAALAMAG
                     GYGRSLDVTVAIQAATIDAAIESWRRWNNAPR"
     gene            6033..>6218
                     /locus_tag="VFF72_02215"
     CDS             6033..>6218
                     /locus_tag="VFF72_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZV92000.1"
                     /translation="MTVTAESTRAVEAAITSRRSIRAFLPTPVPRPVIERILEVASRA
                     PSGTNTQPWKVYVLSGDP"
BASE COUNT          882 a         2122 c         2252 g          962 t
ORIGIN      
        1 agggcctcgg tcgtgccggg cccgacgaga cgtcttggcc ctcgagctcc gggcgaagaa
       61 agggcagctc gctcgcgtca gcggccggcc gccgcttgcc gcccacaggc cgcgcaccga
      121 caccgaccgt gaccacggtc ggtcgcccgg cgtgcagcaa tgccgcgcag gcgaggtcgg
      181 cgatcgtgtg gtcgctggcc agctcgtcgg gccagcccgc acgcgagacg acgagcgccg
      241 ccgtcgccgt ggaccagccc gcgtcgccga gccggcgagc gagcgcgccc agctgcttgc
      301 ccgccatgta gaagacttcg gtgtcggcgt gccgtccggc gccgaggccg ccgtcgtgcg
      361 tcatggccgt ggtgagcgcg acgctgcgac cctggccgcg ccgggtcaac gggcgctgcg
      421 tcgccgctgc cgcggcgagg gccgcggtca ctccgggaac gacctcgcac gcgatgcccg
      481 ccgcgtcgag cgcctcgagc tcttcctcga gacgaccgaa gacgctcggg tctccgccct
      541 tcaggcggac gacgaggcgc gagctggccg cgtgcttgac gagcagggcg ttgatggtcg
      601 cctgaccggt gttctcgccg ccgtcggccg ggccgtgggc gacgccgcga aagccgcgct
      661 tgccgacgtc gatccagcgg gcgcgcggcg ccagcgcgcg cagcgccggg tcggccagcg
      721 aatcgaagag gacgacgtcg gcctcgcgaa gccgggccgc gccgcgaagc gtgatgaggt
      781 cggcctggcc ggggccggcg ccgacgaagg cgacgcggcc tggggcagag gatgtcatgc
      841 cgtcgcctcg acgcgcgcga agcgcaggcg acgttcgtgc gcgtcgcctt gatagaagac
      901 gcgaatgagc ccgagcatcc gctgcgcgac ctccacgctc tcgcccggac gcaggactgc
      961 ggcgtcgaag ccggtgcgat gcagctgcgc ggccatgtcg gcgatcacgt cgcccatggc
     1021 gcgcagttca ccggtgaagc ggtggcgcac gcgcagcagt cgcgcctggc tatacgcgcg
     1081 gccatcggtc catttcggga aacgcagcgc gattcgcccg aaccggccga ggtcggcgag
     1141 cgtctcgtcg atgtcgaggt cgttgggcag tgcgacgctg gcccgaagat cggcaggcca
     1201 gtcggcacga agccgcaacc actcggacca gtcgatgagg gcggtcgtcc cggcctcgac
     1261 ggcgacgttg ccgtcggccc gcggcgaggc atcgtcttcg gcgacgatga actgcatcgc
     1321 tgcggtcgtc attcgacgag ggccggctcg gcgacgtcgc gcctcgcgcg gttcgcggca
     1381 gcgcgaaacg gctcgacccc gagtcggcga aaggtgtcga tgaaacgttc gcccgccccc
     1441 tgtcgccggg ccaggtaggt ctcgatcagc gactcgatga cgtcggggat ctcgctgtcg
     1501 cgaaaggccg gcccgaggac ccggccgggc gccgcggggc cgtggccgcc ggccccgtcg
     1561 gtgccgccga gggtgacctg gtaccactcc tcgccgtcct tgtcgacgcc gagcacgccg
     1621 atgtggccgc tgtggtggtg gccgcaggag ttgatgcagc cgctgatgtt gagcgagatg
     1681 tcgccgatgt cgtgcagctc gtcgagatcg tcgaagcgct cggcgatgtg tgccgatacc
     1741 ggaatcgagc gcgcgttggc cagcgagcag acgtcgcctc cggggcagca gatcatgtcc
     1801 gtcaacaggc cgacgttggg cgtcgcgaaa gagctttcgc gcgcctcgag ccagaggcgg
     1861 tgcaggtcgg cctcgcgcac ccagggcagg acgaggtttt gctggtgcgt gacgcgaagc
     1921 tcgccggcgc tgaaatggtc ggccagctcg gcggcggcgt ccatctggcg atcggtcgtg
     1981 tcgccgggtg cctggccggg ccgcttgagc gagagggtga cggcgcaatg gcccgcaagg
     2041 cggtgcggat gcacgttgcg ctggcgccag cgccggtagg cgggcgactc gtcgagggaa
     2101 gcgacgacgc tcgtggaggc ggcctggtcg gcgacgccct tcggcgtctc gaagtgggcg
     2161 gcgacgcggg ccagctcggc ttcgctgatc aacggcgcga cgccgtcgcc gtcgttgccg
     2221 agaatggccg cgaactcttc ctcgaccttg tgcgtgaatt cggcgccgaa ggccttgacg
     2281 atgaccttga tgcgcgcctt ccagatgttg tcgcggcgac cgaactcgtt gtagacgcgc
     2341 agcaccgcct ccaggtaggc cagcagttgc ggccacggca ggaactcgcg gatgacgctg
     2401 ccgatcatcg gcgtgcggcc catgccgccg ccgacgcgaa cctggaagcc gagctcgcca
     2461 gcctcgttgc gccgcagctg caagccgacg tcgtaccagg cggtcgcggc acggtcttcg
     2521 agcgcgccgt tgacggcgat cttgaacttg cgcggcagtg ccgcgaactc cgggtgcagg
     2581 ctcgaccact ggcgcatgat ttcgcagaac ggccgcggat cggcgacttc gtcgcgggcg
     2641 atgccggcga aggcgtcggc ggtgatgttg cggatgcagt tgccgcaggt ctggatgccg
     2701 tgcatgccga cttcggcgag ctcgtccatc acatcggccg cccgctcgag cgggatccag
     2761 ttgaactgga tgttggtgcg cgtcgtgaag tgaccgtagc ctcggtcgtg gttgcgggca
     2821 atccgcgcca gcgcgcgcat ctgcgtgctg ctgagcgtgc cgtatggaat cgccacccgc
     2881 gccatcggtg catgccgctg cacgtaccag ccgttttgcc gtcgcatgtg gccgaagtcg
     2941 tcagccgaca gcgtgccggc gaggtgacgc gtgagttggt cgcggaactg ggcggcgcgc
     3001 tggcgaacga actggtgatc gaattccgaa tagcggtaca tgcggcgaac gggtggtggc
     3061 gtcgagaatc gacggctcgc gcgcggcgga aagcggcgcc gccagcgaac gccccggctg
     3121 gtgaggaagc gggcactcta ggcggcgggc gccgcagccg caaacacaca ttcgtaggtt
     3181 gctcatcctt gccgtgaata agaagcctcc cctcatctcg ccggcacgtc gtcgcggcct
     3241 tctcgccggc gcaggcggta ccgcgtcgct ggcgatggcg gcgctggcag gctgcgccgg
     3301 ggtcgctccg tcgccgccgg tgccggcact tccaccggcg ccaccgccgc cggccgcacc
     3361 ggccgctccg gcaccgccgc cgcgacggct gcgcgtcggc ctcgcactcg gcggcggcgc
     3421 ggcgcgcggg ttcgcgcaca tcggcgtcat ccaggcgctc gagcaaggcg gcgtcgagat
     3481 cgatctggtc gccggcacgt cggccggcag cctcgtcgcg gcgctgtacg cgtcgggacg
     3541 aaacggtcac gatctcgagc tgcttgccga tgcgatggac gaaaccgcgt tcgccgactg
     3601 gtcgtatccc ggccgaggct tgatccgcgg cgaggcgctg gcgaagttcg tccgcgacaa
     3661 gacgggtggc cggctcatcg agcagatgcg cctgccgctc gggatcgccg cgactgacct
     3721 cgaaagcggc gaggcggttc tgttccgccg cggcgacaca ggcacggcgg tccgcgcttc
     3781 gagcgcggtg cccgcggtgt tccagccggt gaagatcggc agcagggagt acgtcgacgg
     3841 cggactgtcg gcgccggtgc ctgtccacca tgcgatcgcc atgggcgccg agttcgtgat
     3901 cgcggtcgac atttccgccg tcaccgaagg caatgcgacc ggcgacgcct tgcgcatgct
     3961 gctgcagacc ttcacgatca tgggcagcca tatcaaggcc ttcgagctga aggacgccga
     4021 tctcgtcctg cagccgaagc tggcgggtgt ctcgggggct gacttcgcgg ctcggcggca
     4081 caccatcgcg gcgggtcgcg acgtcaccct ggcccatctc gcgcagctgc gcggcctgct
     4141 cgcagccaag agccgtctgt gaggcggcac cagggaagcg gaacgcgaca ggcaaaaaaa
     4201 tggcccggac ggatccgggc cattcactgc gcggccgagg ccgcgcgcgt cttcctcgaa
     4261 ggcttgctta cgcggccttg cgcgacttgg tcgcggcttg cgtcgcgcgg acggcggtcg
     4321 acgacatggc ctggaagttc gcgtcggcga cttcggcggc ctgcttgctc gccttctgca
     4381 cgctttcgaa ggcgttgttg gcggcggcga cggccgactt gacgagcgcc acggcgtttt
     4441 cggtgccggc cggcgcattg cgaacggcgt tatcgacgac ggccatgaac ttcgcctgcg
     4501 cgtcggcggc agtcgcctcg gcgacgcggt tcacttcggc gccggtcgtc gcggcgattt
     4561 cgtagacgtg acggccgtag gcggcggcct tctcggcggt cggctgcatc aggctggcct
     4621 gcagggcgag cagctcctgc gcatccttga ccgacagggc ggcctgggtg gtctcggccg
     4681 cttcggtcag cgccgtgcgg gtggcctgga tgttgagctc gatgatcttc tcgacgcctt
     4741 cgaaggcgag gccgctcagg ccgaagaggg tttcgacgtt ggccttgtgg gcgctcatga
     4801 tctgttcaac ggtcatcatt tcggatctcc tgtgaaaatg gcgggtcgcc tcgggaggcg
     4861 attgatgctg cactgcaaca tagacgaagt atataggtaa accctaggtt tgcaagccgt
     4921 ttatgctgca tcgcaccaca ttagaggacg gcccctacga aggctcgcgg cggcagcgcc
     4981 tgcgcgccgt ccgccgcccg gcccgcaccc tcgccgcgtg aaggcgttcc acagcgacgg
     5041 gcacccgctg ccactgccgg cggggcaccg gtttccgcag tcgaaatacc ggctcttgcg
     5101 gaccctggtc gaaggtcgac ccgatctcga cctcgtcgag gcgccggcgg cgagcgacga
     5161 cttgctggcg ctggctcatt cgcccgccta cgtgcgcagc gtcgccagcg acggcctgac
     5221 gccgctcgag cagcgggcga tcggctttcc gtggagcgag gcgatggtcg agcgatcgcg
     5281 tcgctccgtc ggcgcgacgt gcgaggcggc gcgctcggcg cttcgcgagg gtgtcgccgc
     5341 caacctcgcc ggcggcacgc accacgcgag cgccgaccgg ggcagcggct attgcgtctt
     5401 caacgacgtc gccgtggcat cccggctgat gcaggcgcag gcgtcggcag cgccgctgcg
     5461 aatcgtcgtc atcgacctcg acgtccacca gggcgacggc acggcgcgga tcttcgaagg
     5521 cgatccgagc gtcttcaccg tgtcgctgca tggcgagagg aattttccgt ttcgcaaggc
     5581 gacgagcgat ctcgacgtcg ctttgcccga cgcctgcggc gatgccgact accttgcgtc
     5641 gctcgacgcc gcgctcggcg tcgtgctcgc cgagcacgcg cgggcggcgt tcggcctggc
     5701 gttctacctg gccggcgccg atccgcacga ggacgatcgt ctcggccggc tgaagctgag
     5761 cgccgccggc ctgctcgaga gagaccgccg cgtcctcggg ctgctgcgag aacagcgcat
     5821 cccggccgcc ctggcgatgg ccggcggcta cggcaggagc ctcgacgtca ccgtcgccat
     5881 ccaggccgcg acgatcgacg cggccatcga atcctggcgc cgatggaaca atgcgcctcg
     5941 atgaatccgc atcgatccct cccgagccaa gcgttgccgg cgcccttgcc ggtgccgacg
     6001 ttcgcctgcg cacgcgtgct gaaagaactc gaatgaccgt caccgccgaa tcgacccgcg
     6061 ccgtcgaggc cgccatcacc tcgcgacgct cgatccgagc cttcctgccg acgccggtgc
     6121 ccaggccggt catcgagcgc atcctcgagg tcgcctcgcg ggcaccgtcg ggaacgaaca
     6181 cgcagccgtg gaaggtctat gtgctctcgg gcgatccg
//
LOCUS       DASRWS010000124         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1772192, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000124 DASRWS010000000
VERSION     DASRWS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772192"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFF72_02220"
     CDS             complement(<1..287)
                     /locus_tag="VFF72_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HZV92001.1"
                     /translation="MEAQIEHVRRELGGVRTGRASVNILDSVHVEAYGSQMPLNQVAS
                     LSIPEPTLIVAQPFDPSLMAAIEKAIRSSNLGLNPTSDGKAIRVPIPALTE"
     gene            complement(349..1065)
                     /gene="pyrH"
                     /locus_tag="VFF72_02225"
     CDS             complement(349..1065)
                     /gene="pyrH"
                     /locus_tag="VFF72_02225"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZV92002.1"
                     /translation="MPPAYRRVLLKLSGEALMGEQAFGIDPGVTTQIAKDIAEIQQLG
                     VQAAVVIGGGNLFRGLAASAKGMDRATADYMGMLATVINALALQDALEHVGVNTRVAT
                     AIEMRAVAEPFIRRRAVRHLEKGRVVVFAAGTGNPYFTTDTAAALRAMEMKADVILKG
                     TKVDGIYTADPMLHPTATKFESISYIKVLEQGLKVMDATAISLCMDNRLPIVVFNLRT
                     PGNLKRVIMGDAVGTVVSAA"
     gene            complement(1087..1680)
                     /gene="tsf"
                     /locus_tag="VFF72_02230"
     CDS             complement(1087..1680)
                     /gene="tsf"
                     /locus_tag="VFF72_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZV92003.1"
                     /translation="MDITAAMVKTLRDATGAGMMECKAALAEAKGDMDEATTLLRKRG
                     LAQAAKKAGRSTSEGLIGHYIHMGGKIGVLVEVNCESDFVARTDAFQGLVKELAMHIA
                     AANPSYVRRDEVPGDVLAREREIYREQVKDKPAQVVDKIVEGKLESYYEQFCLMDQFS
                     IRDPKTRIRDVVQAAIAKLGENITITRFVRMKVGEAA"
     gene            complement(1845..>2508)
                     /gene="rpsB"
                     /locus_tag="VFF72_02235"
     CDS             complement(1845..>2508)
                     /gene="rpsB"
                     /locus_tag="VFF72_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZV92004.1"
                     /translation="TAKLFRDAEEFVTNLAADGRTVLFVGTKRQAQDAIAEEAQRSGM
                     FFVNQRWLGGLLTNFTTIQRSLARLRDLEAMETDGRYETLSKKEIAQLEKEKRKLQKN
                     LEGIRHMNRLPDALFVVDTRKEKIAVDEARKLKIPVIGVVDTNCDPDEVDFVIPGNDD
                     ALRAIRLFASKIAEAVIAGRGIAESSRGDDSDGEGDDRGRRSRPNRPAARAEQATTPA
                     SV"
BASE COUNT          362 a          848 c          813 g          485 t
ORIGIN      
        1 tcctcggtca gcgcgggaat cgggacccgg atcgctttcc cgtcgctcgt cgggttcagg
       61 ccaagattgg agctgcggat ggctttctcg attgccgcca tcaggctcgg atcgaacggc
      121 tgcgcgacga tcagcgtggg ctcgggaatg gagagcgacg ccacctggtt gagcggcatc
      181 tgcgaaccgt aggcctcgac gtgcaccgag tcgagaatgt tgaccgaggc gcgtcccgtg
      241 cggacgccgc ccagctcgcg gcgcacgtgc tcgatctgcg cctccatccg cttcttgacc
      301 tcggcgaaca ggctcttgac gtcatttaca tccatagaat ccccaccctt acgcagcgct
      361 cacgacagtc ccgacggcgt cgcccatgat gacgcgcttc aggttgccgg gcgtgcgcag
      421 gttgaacacc acgatcggca ggcggttgtc catgcacagc gagatcgccg ttgcatccat
      481 caccttgagc ccttgctcga gcaccttgat gtagctgatc gactcgaact tcgtcgccgt
      541 ggggtggagc atcgggtccg cggtgtagat gccgtcgacc ttcgtgccct tcaggatcac
      601 gtcggctttc atctccatcg cgcgcagcgc ggccgccgtg tcggtcgtga aatacgggtt
      661 gccggtgccg gcggcgaaga cgacgacgcg ccccttttcg aggtgacgca ccgcccgccg
      721 ccggatgaac ggctcggcga cggcgcgcat ctcgatggcg gtggcgacgc gggtgttgac
      781 gccgacatgt tcgagcgcgt cctgcaacgc cagcgcgttg atgacggtcg ccagcatgcc
      841 catgtagtcc gccgtcgcgc gatccatgcc tttcgcgctg gcggcaagac cgcgaaagag
      901 attgcccccg ccgatgacca ccgcggcctg gacgccgagc tgctggatct cggcgatgtc
      961 cttggcgatc tgggtggtga cgccgggatc gattccaaac gcctgttcgc ccatgagtgc
     1021 ttcgccggag agcttgagca ggacgcgacg gtaggccggc ggcatggctg tcaattatat
     1081 gagcaactac gccgcttccc cgaccttcat ccgcacgaac cgcgtgatcg tgatgttctc
     1141 gcccagcttg gcgatcgcgg cttgcacgac gtcgcgaatg cgggtcttcg ggtcgcggat
     1201 ggagaactgg tccatcaggc agaactgctc gtagtagctc tccagctttc cttcgacgat
     1261 cttgtcgacg acctgcgccg gcttgtcctt cacctgttcg cgatagatct cgcgctcgcg
     1321 cgcgagcacg tcacccggca cttcgtcgcg ccggacgtag ctggggttgg cggccgcgat
     1381 gtgcatcgcc agctccttca cgagcccctg gaaggcgtcg gtgcgcgcga cgaagtccga
     1441 ttcgcagttc acttcgacga ggacgccgat cttgccgccc atgtggatgt agtggccgat
     1501 cagtccttcc gacgtcgagc ggcccgcctt cttggccgcc tgcgcgaggc cgcgcttgcg
     1561 gagcagggtc gttgcctcgt ccatgtcgcc cttggcctcc gccagggccg ccttgcactc
     1621 catcatcccc gcgccggttg catcgcggag cgtcttcacc attgccgctg tgatgtccat
     1681 atgtgtcttc tctcaatgct cgcggggccc cacccccgct cgcgctcgct cggcggtcgg
     1741 cctgcggccg actgcactcc gtcgcctcgc tcgggccgca ggcgcttgta catgaaaacg
     1801 ccggcctcgg gaatcccggc cggcgcgtcg aaagcgtgcg gcggtcagac cgacgcgggg
     1861 gtcgtggcct gttccgcgcg ggcggccggg cggttcggcc gcgagcggcg gccgcgatcg
     1921 tcgccttcgc cgtccgagtc gtcgccgcgc gaggactcag cgatgccgcg gccggcgatc
     1981 accgcttcgg cgatcttcga cgcgaacaac cggatcgccc gcagcgcatc gtcgttgccg
     2041 gggatcacga agtcgacttc gtcggggtcg cagttggtgt cgacgacgcc gatgaccgga
     2101 atcttcagct tgcgcgcctc gtcgaccgca atcttctcct tgcgggtatc gacgacgaag
     2161 agggcgtcgg ggagccggtt catgtggcgg atgccttcga ggttcttctg gagcttgcgc
     2221 ttctccttct cgagctgcgc gatctccttc ttcgagagcg tctcgtagcg gccgtcggtc
     2281 tccatcgcct cgagatcccg caggcgggcg agactgcgct gaatcgtcgt gaagttggtg
     2341 aggagccccc cgagccaccg ctggttcacg aagaacatgc ccgaacgctg cgcttcctcg
     2401 gcgatcgcat cctgcgcctg gcgcttcgtg ccgacgaaca gcacggtgcg cccgtcagcg
     2461 gcgaggttgg tcacgaactc ctcggcgtcc cggaacaact tcgccgtc
//
LOCUS       DASRWS010000125         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_47926, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000125 DASRWS010000000
VERSION     DASRWS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47926"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VFF72_02240"
     CDS             complement(<1..118)
                     /locus_tag="VFF72_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZV92005.1"
                     /translation="MRTSAPGSETLRRESARAGGFTLLELMVVVAIIAIATGV"
     gene            complement(93..527)
                     /gene="gspG"
                     /locus_tag="VFF72_02245"
     CDS             complement(93..527)
                     /gene="gspG"
                     /locus_tag="VFF72_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HZV92006.1"
                     /translation="MHSLTKRPRRGRGFTLIELMVVLVIIGVLAALIVPNMLDRAEDA
                     RVTAARTDVNNLMQALKLYKLDNLRYPSGEQGLQALVSKPTTGTIPPNWRPYLDKLPS
                     DPWGHPYQYENPGLKGEIDVYSFGADGVVGGEGRNADIGSWQ"
     gene            586..1248
                     /locus_tag="VFF72_02250"
     CDS             586..1248
                     /locus_tag="VFF72_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92007.1"
                     /translation="MFARLSAFVIWSLVAATVVFWSLRLGVRSPPPPAYAVPAERSTP
                     LRGDLARLFGAAPVLASATDSRPEAPSRYRLLGVMAPRSSSAAAHDYGFALIAIDGKP
                     ARAYSVGAPLDSGLVLKSVGLRTASLGPADGGAQSMLLELPRLPTPATGVLPVPGARM
                     GTPIIARPVSPVNEAMTRAAPSVQPVPGVNSRPGAMEGETAPSRPEAGNGLPAADDFR
                     SQ"
     gene            complement(1297..2865)
                     /gene="ilvA"
                     /locus_tag="VFF72_02255"
     CDS             complement(1297..2865)
                     /gene="ilvA"
                     /locus_tag="VFF72_02255"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HZV92008.1"
                     /translation="MALNSRKSHLRRFAPLPATVLSGYLQKVLTARVYDVALESALDE
                     ASRLSDRLGHRVLLKREDTQPVFSFKLRGAYNKMARLPAEALARGVICASAGNHAQGV
                     ALAARHLGCRSLVVMPTTAPSLKIASVRALGAEVVLHGDSYSDAYAYARVLEATGKLT
                     FVHPFDDPDVIAGQGTIAMEILRQQPGPIDAIFVAVGGGGLISGIATYVKAVRPDIRV
                     VGVQMSDSDAMLRSIRAGRRVELSDVGLFSDGTAVKRVGAETFRLASRLVDDWVVVDT
                     DAACAAIKDVFEDTRSILEPAGALGVAAIKQYAQHRRRKGETYVAITCGANMNFDRLR
                     FVAERAEVGEEREALFAVTIPEARGSFRRFCEVLGPRAVTEFNYRISDARVAHVFVGI
                     ATVDRAESARLARDFASHGFATLDLTGDELAKEHVRHMVGGRSGLAVDERLYRFVFPE
                     RPGALMRFLSSMPAGWNISLFHYRNQGADYGRILVGMQVPRADRHAFSEFLESLDYPC
                     AEETDNPAYRLFLQ"
     gene            3144..3560
                     /locus_tag="VFF72_02260"
                     /pseudo
     CDS             3144..3560
                     /locus_tag="VFF72_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            complement(3672..4313)
                     /gene="coq7"
                     /locus_tag="VFF72_02265"
     CDS             complement(3672..4313)
                     /gene="coq7"
                     /locus_tag="VFF72_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HZV92009.1"
                     /translation="MHTRASSPTDRVLRFADQALRALTTTPVPARPMPRPPRDDVALD
                     ATQRRESGALMRVNHVGEVCAQALYAAQALTTPSPALRRQFEAAAADETDHLAWTSER
                     LRELGARPSLLNPAWYAGAFAIGLLAGRAGNAASLGFVVETERQVEQHLQRHLDRLPV
                     SDLASRAVVSQMKDDEARHASTAEQAGAKRLPWPVRLAMRAAAGLMTTTAHYV"
     gene            4528..>5271
                     /locus_tag="VFF72_02270"
     CDS             4528..>5271
                     /locus_tag="VFF72_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZV92010.1"
                     /translation="MKKSLLALAVLGTFAGAASAQSSVTIWGVVDLSLNSVKNGDTTS
                     RTMQSNQLNSNRLGFRGVEDLGGGMKAGFWLEAGMANENGTVGGSNGLAAPGAGTSFF
                     NRRSTVSLFTPMGELRLGRDYDPSFWNTVFDDVDGANGLGQGLNLISSLGSGAITIAR
                     DNNSIGYFLPSNLGGLYGQAMVAAGNNVPGQKYYGGRLGFATGPFDVNAGYGDTKTAV
                     SSDFKMWNVGGSYDFGFLKLYGYYNHNSFQ"
BASE COUNT          864 a         1709 c         1798 g          900 t
ORIGIN      
        1 ccacgcccgt ggcgatggcg atgatggcga cgaccaccat gagctcgagc agcgtgaagc
       61 cgcccgcccg ggcggattcc cggcgaagag tctcactgcc aggagccgat gtccgcattg
      121 cggccttcac cgccgacgac gccgtcggcg ccgaagctgt agacgtcgat ctcgcctttc
      181 aggcccgggt tttcgtactg gtacggatga ccccacggat cgctcggcag cttgtcgaga
      241 tagggtctcc agttcggcgg aatcgtgccc gtggtcggtt tgctcaccag cgcctgcagc
      301 ccctgttcgc cgctcggata gcgcaggttg tcgagcttgt aaagcttgag cgcctgcatg
      361 aggttgttga cgtcggtgcg cgcggcggtg acgcgcgcat cttcggcccg gtcgagcatg
      421 ttgggcacga tcagcgccgc cagcacgccg atgatgacga ggacgaccat cagctcgatc
      481 agcgtgaagc cgcgaccgcg gcgaggtctt ttcgtcaagg aatgcatcgt ggagttctac
      541 cgttcggaca ccttggcggc gaggtcgttc catcataatc gccggatgtt cgcgcgcctt
      601 tcggcattcg tgatctggtc gctggtcgcg gcgacggtcg tcttctggag ccttcggctg
      661 ggcgtacgtt cgccgccgcc tcccgcctac gccgttccgg ccgagcgctc gacgccattg
      721 cgcggcgatc tggcgcgtct cttcggcgcg gctccggtgc ttgcgtcggc caccgattcg
      781 cgtcccgagg cgccgtcgcg ctaccgcctg ctcggcgtca tggcgccgag atccagttcg
      841 gccgccgcgc acgactacgg ctttgcgctg atcgccatcg acggcaagcc ggcgcgtgcc
      901 tacagcgtcg gcgcgccgct cgacagtggt ctagtgttga agtcggtggg ccttcgcacc
      961 gcctcgctcg ggcccgcaga cggcggcgct caaagcatgc tgctcgagct gccgcggctg
     1021 ccgacgccgg cgacgggtgt cctgccggtc ccgggcgcga ggatgggcac gccgataatt
     1081 gcgaggcctg tttccccggt caacgaggcg atgacccggg ccgcgccatc cgtccagcct
     1141 gtgccgggcg tcaattcccg gcccggggcg atggagggcg agacggcacc gtcgcggccg
     1201 gaggcgggca acggcctgcc ggccgccgac gactttcgct cgcaatagcg agcggcgccg
     1261 caggtcgctc ggcccctgac ggggacgccg ctgcggctac tgcaggaaca atcgataggc
     1321 cgggttgtcg gtctcctcgg cacacgggta gtcgagcgat tcgaggaatt cggaaaatgc
     1381 gtgacggtcg gcccggggca cctgcatgcc gaccaggatc cgcccgtagt cggcgccctg
     1441 gttccgatag tggaagaggc tgatgttcca gccagcgggc atgctcgaga ggaagcgcat
     1501 cagcgctccc ggacgctcgg ggaaaacgaa ccggtagagg cgctcgtcga ccgcgagtcc
     1561 gctgcgcccg cccaccatgt gacggacgtg ctccttcgcc agctcgtccc cggtgaggtc
     1621 gagcgttgcg aacccgtggc ttgcgaaatc gcgcgcaagg cgcgccgact cggcacgatc
     1681 gaccgtcgcg atgccgacga agacatgggc gaccctggcg tccgaaatcc ggtagttgaa
     1741 ctcggtgacg gcgcgcggcc cgaggacttc gcagaagcgc cgaaagctgc cgcgcgcctc
     1801 ggggatggtg acggcgaaga gggcttcgcg ctcctcgcca acctcggcgc gttcggccac
     1861 gaagcgcagg cgatcgaagt tcatgttcgc accgcacgtg atggcgacgt aggtctcgcc
     1921 cttgcgccgg cggtgctgcg catactgctt gatcgcggcg acgccgagcg cgccggccgg
     1981 ctcgagaatg ctgcgcgtgt cttcgaagac gtccttgatc gccgcgcagg ctgcgtcggt
     2041 gtcgacgacg acccagtcgt cgacgagacg actcgccagg cgaaacgtct cggcgccgac
     2101 gcgcttgacg gcagttccgt cggagaaaag gccaacatcg gaaagctcga cgcggcgccc
     2161 ggcgcggatc gagcgcagca tcgcgtcgga atcgctcatc tgaacgccga cgacgcgaat
     2221 atcggggcgt accgccttga cgtaagtggc gatcccggag atcagcccgc cgcctcccac
     2281 cgccacgaag atcgcatcga tcggacccgg ctgctggcgc aggatctcca tggcgatcgt
     2341 gccctggccg gcgatcacgt cgggatcgtc aaacgggtgc acgaaggtga gcttgcccgt
     2401 cgcttcgaga acgcgtgcgt aggcgtaggc gtcggagtag ctgtcgccgt gcagcaccac
     2461 ctccgctccg agcgcccgca ccgaagcgat cttcagggag ggcgcggtcg tcggcatcac
     2521 caccagcgac cggcagccca gatggcgagc cgcgagcgcg acgccctggg cgtggttgcc
     2581 cgccgaagcg cagatgacgc cgcgcgccag cgcttcggca ggcagccggg ccatcttgtt
     2641 gtaggcgcca cgcagcttga agctgaagac cggctgcgtg tcctcgcgct tgaggagcac
     2701 gcgatgcccg agacggtccg acaggcgcga agcttcgtcg agtgcggatt cgagcgccac
     2761 gtcgtagacg cgcgcggtga gcaccttctg cagatatccc gacagcacgg tcgctgggag
     2821 cggtgcgaat cgacggaggt gcgacttcct ggagttcaat gccatgcccg tggcgctttc
     2881 gaccgtcggc gatatgcggc gctgcatcat aagcgtcggt cgcgacgcgg ttcctgggtg
     2941 gcgggaattc gccgcggcgt accgcgtcgc actggaaata aaagggcccg agactttgca
     3001 gccccgggcc gaatccacca atggaggagg tggaggagac aatcggtttg accgccggat
     3061 gcgccaggcc acgggccgga tgtcgattcg aaagtcagtt tagggtcttt tttgctgcag
     3121 tgcaatatga gaaggcgtac ccgatgaagt gcacgatcga ctggatgccc gccagcggca
     3181 tggccttttg tgccgagacc ggaagcggtc acatgctgac gatggacggc gcgccggacg
     3241 gaggcggtcg caacctggcg ccgcggccga tggagaccgt tctcgcaggc acgggcgcct
     3301 gcacggctta tgacgtcgtc ctcatccttc gtcgcggccg gcacgacgtt cggggctgcc
     3361 ggctggaagt gagctcgcaa cgtgccaaag tcgatccgaa ggtcttcacg cgcatcgagc
     3421 tgcacttcgt ggtcaccggc gtcggactgt cggcatcggc ggtcgagcgg gccatcgccc
     3481 tgtcgcacga gaaatactgc tcggcgacgg ccatgctcgg caagaccgcc gagatcgtca
     3541 ccagcttcga ggtcgtcgaa accggtgtcg ctggggcggc gacggacggt acttccggaa
     3601 cggacatgcg aaccttcgtt ccgccgggcg ttgccggctg acgccggagg cgcggctcgc
     3661 gaagggtcag gtcacacgta atgcgccgtc gtcgtcatca agccggccgc ggcgcgcatc
     3721 gcgagcctga ccggccaggg caggcgcttg gcacccgcct gttccgcagt ggacgcgtgc
     3781 cgcgcctcgt cgtccttcat ctgcgagacg acggcgcgtg aagcgaggtc ggaaacagga
     3841 agccggtcca gatgccgttg aaggtgttgt tcgacctgcc gctccgtttc gacgacgaag
     3901 cccagactgg cggcatttcc agcccggccc gccagaagtc cgatcgcgaa tgcgccggca
     3961 taccaggccg gattgagcag gctggggcgg gcgcccaatt cgcgcagccg ttcgcttgtc
     4021 cacgcgaggt gatcggtttc gtcggccgcg gccgcctcga actggcgcct tagagctggg
     4081 ctcggtgtcg tcagcgcctg cgcggcataa agcgcctgcg cgcagacctc gccgacgtgg
     4141 ttgacgcgca tcagcgctcc tgactcgcgc ctctgggtgg cgtcgagggc cacgtcatcg
     4201 cgaggcggcc gcggcatcgg cctggcgggc acgggggtgg tcgtgagggc gcgcaaggcc
     4261 tgatcggcga agcgcagcac gcggtctgtc ggcgaagaag cgcgggtgtg catgccactg
     4321 gagaataccc gtgccccggt gttacatgcc gcgaaatcct cgttgtgctg agacaacatt
     4381 aggcgttttt ttgcgccgca aggctttgca gggcgaggtc cctctggtgc aatacacaca
     4441 acttccgcaa agggggttgt acctgatctg ctccagcgcg ggtttttggt gatcgggacc
     4501 tcttgttcaa cctaggagat agtcgcaatg aaaaaatctc tgctcgctct cgctgtcctc
     4561 ggcacattcg ctggcgcggc ctcggcgcag tcttccgtga cgatttgggg cgtcgtcgac
     4621 ctgagcctga atagcgtcaa gaacggcgac accacgagtc gcacgatgca aagcaaccag
     4681 ctcaacagca accgcctcgg ctttcgcggt gtcgaagacc tcggcggcgg catgaaggca
     4741 ggcttctggc tcgaggccgg catggccaac gagaacggca ccgtgggcgg cagcaatggc
     4801 ttggccgctc cgggcgcagg cacatcgttc ttcaatcgtc gttccaccgt cagcttgttt
     4861 accccgatgg gtgaactgcg gctcggtcgc gactacgatc cgtccttctg gaacacggtg
     4921 ttcgacgacg tcgacggcgc caacggtctg ggccagggcc tgaacctgat cagctcgctc
     4981 ggcagcggtg cgatcaccat cgctcgcgac aacaactcga tcggctactt cctgccgagc
     5041 aacctcggtg gcctgtacgg ccaggcgatg gtcgccgcag gcaacaatgt ccccggccag
     5101 aagtactacg gcggccgtct cggcttcgcg accggtccgt tcgacgtcaa cgctggctat
     5161 ggcgacacga agactgccgt ctcgagcgac ttcaagatgt ggaacgtggg cggctcgtac
     5221 gacttcggat tcctgaagct ttacggctac tacaaccaca actcgttcca g
//
LOCUS       DASRWS010000126         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1200165, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000126 DASRWS010000000
VERSION     DASRWS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200165"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1005
                     /locus_tag="VFF72_02275"
     CDS             226..1005
                     /locus_tag="VFF72_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZV92011.1"
                     /translation="MLTRDRVAAGAVVPSAGVSPLVAERLSDRLAERLGREIGGGQLR
                     PGDRLPTEQRLAAAHGVSRTVVREAVHQLKSRGLLRSRQGSGVYVTAAPAGEALTLDL
                     SVIGSIDDVMRIREVRRALEAETAALAAERATRAQVASLRRALRAIDQSAAQGRDAVD
                     EDLAFHRLLAEAAGNPHFVRLLEFLEQYALEAMRVTRRNDLTRPDFVEAVRKEHRAIV
                     DAVALGDPALARRRALQHMRGGDRRMRTAAPISSTAKAARR"
     gene            1002..2015
                     /gene="denD"
                     /locus_tag="VFF72_02280"
     CDS             1002..2015
                     /gene="denD"
                     /locus_tag="VFF72_02280"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HZV92012.1"
                     /translation="MKILITGGAGFIGSRLARALLDRGSLAGHPIDRLVLADQVAPRA
                     ELAADGRVETRVGVLAAQCEALAADGFDGVFHLASAVSGECEADFDLGLRSNLDTTRA
                     LLDALRGTARAGRPPARLVFSSSVAVFGPDPSKPLPEQVGDDTLPAPKTSYGTHKLMC
                     EHLIADYSRKGFIDGRSARLMTVTVRPGRPNGAASSFFSGIVREPLAGVESICPVGPE
                     VSHPLTSAARTVEGLIAVYEASIEALGGRLALNLPAVNATVAQMLDALEAVAGKAVRD
                     RVRFVRDETIAGIVANWPRGATAARAARLGLRADADFQTIIRRYIDDCRAAGDDAALK
                     GLA"
     gene            2012..2764
                     /locus_tag="VFF72_02285"
     CDS             2012..2764
                     /locus_tag="VFF72_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV92013.1"
                     /translation="MNRIDLTDRVAIVTGGAGDIGHAIAERMLASGARVVLWDADTGK
                     LEEAKARHGGAATVSTRTVELTREDDVAAATEEAMRAHGRIDIVVNNAGITGGNATTW
                     QLEPEVWRRVIEVNLVAPYLVCRAVVPKMLASGYGRIVNIASIAGKEGNPNASHYSAS
                     KAGLISLTKSLGKELAGKNILVNAVTPAAAKTAMFASMTQQHIDFMLSKIPMGRFLEV
                     GEAAAMVAWLASEECSFTTGAVFDLSGGRATY"
     gene            2823..>3800
                     /locus_tag="VFF72_02290"
     CDS             2823..>3800
                     /locus_tag="VFF72_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV92014.1"
                     /translation="MIFEQVQSGGCRSYLIGCEASCAAALIDPEVSQIDRYLALATKH
                     GLRIRFVIDTHTHADHFSATRQLAQRFGAPTVMHRASPAPGIDIHVDDGEMIIVGQLR
                     VRVLHTPGHTQDSMSLVVEDRLFTGDTLLIGGTGRTDLPSGDPNALYDSLFGRLLKLD
                     PALRVHPAHEYKGRAESTLAAEIETNPRLQQREREAFVAMMKGLELTMPVHITEALRT
                     NMSGGKTVTQMLAEAAAQVSFMSLAELNTRVGSGSCDLIVLDVRERDAYETAHVPGAR
                     LLPRGQLELRVNEELPDPTRRIVTCCDFGRISTLAAATLREMGFMRTVAL"
BASE COUNT          562 a         1449 c         1276 g          513 t
ORIGIN      
        1 accagcacgg tgaacgagcc gtcagcgatg tcgatgtcga cgccatgcag cacggccgtg
       61 tggccgaagc tctttttcac cgactggatc gtcacactgg ccatcgacgc tcgaggaagc
      121 cacccacggg ggttgcgcga agtatctgca tcggccggcg cccccgccat gagggcaaac
      181 cggtagacgc ggccgccgcc ggattcgtat gatgacccga ccaccatgct gacccgagac
      241 cgtgtcgccg ccggcgccgt cgtgccttcg gccggcgtct ctcccctcgt cgccgagcgc
      301 ctgtccgacc gcctcgccga gcgcctgggc cgcgagatcg gcggcggcca gctgcgcccc
      361 ggcgatcgcc tgcccaccga gcagcgtctg gccgccgcgc acggcgtctc gcgcaccgtc
      421 gttcgcgagg cggtgcacca gctcaagtcg cgcggcctgc tgcgctcgcg ccagggctcg
      481 ggcgtctacg tcaccgccgc gcccgccggc gaagcgctga cgctcgatct gagcgtgatc
      541 ggctcgatcg acgacgtgat gcgaatccgc gaggtgcggc gcgccctcga ggccgagacc
      601 gcggcactcg ccgccgagcg ggcgacgcgg gcgcaggtcg ccagcctgcg ccgcgccctg
      661 cgcgccatcg accagagcgc ggcgcaaggc cgcgacgccg tcgacgaaga cctggccttc
      721 catcgcctgc tggccgaggc ggccggcaac ccgcacttcg tccgcctgct cgaattcctc
      781 gagcagtacg cgctcgaggc gatgcgggtg acccgccgca acgacctgac gcggcccgat
      841 ttcgtcgagg cggtacgcaa ggagcaccgc gccatcgtcg atgcggtcgc cctcggcgac
      901 ccggcgctgg cgcgtcgccg ggcgctgcag cacatgcgcg gcggcgatcg ccggatgcgc
      961 accgccgcac cgatctcctc caccgcgaag gcagcccgac gatgaagatc ctgatcaccg
     1021 gcggcgccgg attcatcggc tcgcggctgg cccgcgccct gctcgaccgc ggcagcctcg
     1081 ccggccaccc gatcgaccgg ctggtccttg ccgaccaggt ggcgccgcgc gccgagctgg
     1141 ccgccgacgg ccgggtcgag acccgcgtcg gcgtcctcgc cgcccagtgc gaagcgctcg
     1201 ccgccgatgg cttcgacggc gtcttccacc tcgcctccgc cgtctcgggc gaatgcgaag
     1261 ccgatttcga cctcggcctg cgctccaacc tcgacacgac gcgcgccctc ctcgacgcgc
     1321 ttcgcggcac ggcccgcgcc ggccggccgc cggcgcgcct cgtcttctcg agctcggtgg
     1381 cggttttcgg gcccgacccg tcgaagccgc ttcccgagca ggtcggcgac gacacgctgc
     1441 cggcgccgaa gacctcgtac ggcacgcaca agctgatgtg cgagcacctg atcgccgact
     1501 attcgaggaa gggcttcatc gacgggcgct cggcccggct gatgacggtg acggtgcggc
     1561 ccggccggcc gaacggcgcc gcgtcgtcgt tcttctccgg catcgtccgc gagccgctcg
     1621 ccggcgtcga atcgatctgc ccggtcggcc ccgaggtctc gcatccgctc acctcggccg
     1681 cgcgcaccgt cgaggggctg atcgccgtct acgaggcgag catcgaggcc ctcggcggcc
     1741 ggctcgccct caacctgccc gccgtcaacg ccaccgtcgc gcagatgctc gacgcgctcg
     1801 aggcggtcgc cggcaaggcg gtgcgagacc gggtccgctt cgtgcgcgac gagacgatcg
     1861 ccggcatcgt cgccaactgg ccgcgcggcg ccacggcggc gcgtgcggcg cggctcggcc
     1921 tgcgcgccga cgccgatttc cagaccatca tccgccgcta catcgacgac tgccgcgccg
     1981 ccggcgacga cgcggcgctc aagggactcg catgaaccgg atcgacctca ccgaccgcgt
     2041 cgccatcgtc accggcggcg ccggcgacat cggccacgcc atcgccgagc gcatgctcgc
     2101 gtcgggcgcc agggtcgtcc tctgggacgc cgacaccggc aagctcgaag aagcgaaggc
     2161 gcgccacggc ggcgccgcga ccgtctcgac gcgcaccgtc gagttgacgc gcgaagacga
     2221 cgtcgccgcc gccaccgaag aggcgatgcg cgcgcacggc cgcatcgaca tcgtcgtcaa
     2281 caacgccggc atcaccggcg gcaacgcgac gacgtggcag ctcgagcccg aggtctggcg
     2341 gcgcgtcatc gaggtcaacc tggtggcgcc ctacctggtc tgtcgcgcgg tcgtgccgaa
     2401 gatgctggct tcgggctacg gccgcatcgt caacatcgcc tcgatcgccg gcaaggaagg
     2461 caacccgaac gcctcgcact acagcgcgtc gaaggccggc ctgatctcgc tcaccaagtc
     2521 gctcggcaag gagctcgccg gcaagaacat cctggtcaac gcggtgacgc cggcggcggc
     2581 gaaaacggcg atgttcgcgt cgatgacgca acagcacatc gacttcatgc tctcgaagat
     2641 cccgatgggc cgctttctcg aggtcggcga ggccgcggcg atggtcgcct ggctggcctc
     2701 ggaggaatgc tcgttcacca ccggcgccgt cttcgatctc tcgggaggcc gggcgaccta
     2761 ttgatacgat cccccgatgc gcacgcgggc ccgcgaccgg gccgctctcc ggggaaccga
     2821 ccatgatctt cgagcaggtg caaagcggcg gctgccgctc ctatctcatc ggctgcgaag
     2881 cgagctgcgc cgccgcgctc atcgaccccg aggtcagcca gatcgaccgc tacctggcgc
     2941 tggcgacgaa gcacggcctg cggatccgct tcgtcatcga cacgcacacc cacgccgatc
     3001 atttctcggc cacgcgccag ctcgcccagc gcttcggcgc gccgacggtg atgcaccggg
     3061 ccagcccggc gccgggcatc gacatccacg tcgacgacgg cgagatgatc atcgtcggcc
     3121 agttgcgggt gcgcgtcctg catacgccgg ggcacaccca ggattcgatg agcctcgtcg
     3181 tcgaggatcg cctcttcacc ggcgacacgc tgctgatcgg cggcaccggc cgcaccgacc
     3241 tgccgagcgg cgacccgaac gcgctctacg acagcctgtt cggccgtctc ctcaagctcg
     3301 acccggccct gcgcgtccat ccggcgcacg aatacaaggg ccgcgccgag tcgacgctgg
     3361 ccgcggaaat cgagaccaac ccgcgcctgc agcagcgtga gcgcgaagcc ttcgtcgcca
     3421 tgatgaaggg cctcgagctg acgatgccgg tgcacatcac cgaggcgctg cgcacgaaca
     3481 tgagcggcgg caagacggtg acgcagatgc tcgccgaggc ggcggcgcag gtgagcttca
     3541 tgtcgctggc cgagttgaac acgcgagtcg gcagcggctc gtgcgacctg atcgtcctcg
     3601 acgtgcgcga gcgcgacgcc tacgagacgg cgcacgtgcc cggcgcgcgg ctgctgccgc
     3661 gcggccagct cgagctgcgc gtcaacgagg agctgcccga cccgacccgg cgcatcgtca
     3721 cctgctgcga cttcggccgc atctcgacac tcgccgccgc gacgctgcgc gagatgggct
     3781 tcatgcgcac ggtggcgctc
//
LOCUS       DASRWS010000127         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3792879, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000127 DASRWS010000000
VERSION     DASRWS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792879"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..140
                     /locus_tag="VFF72_02295"
     CDS             6..140
                     /locus_tag="VFF72_02295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HZV92015.1"
                     /translation="MFALGLCAAWAYDRSRLLMAPMIVHAIYNAAVIGWQNWVLVPPG
                     "
     gene            1239..2339
                     /locus_tag="VFF72_02300"
     CDS             1239..2339
                     /locus_tag="VFF72_02300"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZV92016.1"
                     /translation="MNAPFRADQVGSLLRPAELSRARRRLKAGELDADALSAIEDRAI
                     TEAVRRQQDIGLQGITDGEFRRDWWHLDFLARLDGVALQDNLGPKFNAEGHEQPPIAT
                     VTGKVACSKPIMVDDFAFLKSVTTGTPKMTIPSPSMLHLRGGRAAISKQAYPDLAEFW
                     SDVATAYRDAIGHLYDAGCRYLQLDDITFAYLCDAKIQSNCRANGDDPALLPRIYADT
                     INAALAGKPDDLRVTIHTCRGNFKSAWVAQGGYAPVVEAMFSTDVDGYFMEFDSERAG
                     GFEPLHVLQDSGDKAKKVVLGLVTTKVGALESKDELKRRIDEAARALPLERLCLSPQC
                     GFSSTHHGNALSPDEQWHKLERVVEVAHDVWG"
     gene            2357..3199
                     /locus_tag="VFF72_02305"
     CDS             2357..3199
                     /locus_tag="VFF72_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HZV92017.1"
                     /translation="MQLPWRVLSCLATIFCVGMAHGQAAPGDLPLLRQGNFSRLEAET
                     VSLQQRFATGQVTEFGLRQFYRSFYDLTEQDLARLDEWQAAYPRSYAVRLVRGTYYKL
                     AAFKARGGNFAANTPPESFAEMERLLARARPDLEASLSLTEKPLLSVFHLLDMTDGNR
                     AVRKALMDSGTRMLPSNRLIRGRYMFGLTPRWGGSYEEMKAFLAYAKRTGASKVGLME
                     MEALMYDDMAEVALHQGDQESAVVNFRKALELNAKVGPDGFGEPPFSKRYKCRLPALR
                     EYCR"
BASE COUNT          624 a         1131 c         1081 g          541 t
ORIGIN      
        1 tgccggtgtt cgcgctgggg ctgtgtgcgg cttgggccta cgatcgctca cgcctgctga
       61 tggcgccgat gatcgtgcac gcgatctaca acgctgccgt gatcggatgg cagaactggg
      121 ttctggtacc gcctggctaa cgtcgacttg cggctacagc ggtcgctcat aagccaatcc
      181 cgaccgacag catgtagtct gaagccgaga tctgcattgg ctgcccagaa gtgcgccccg
      241 ctgcagctcg atgccgatca ggcactctgt acccagctca cccctttgca agtcggtggt
      301 cgaacttaac cagcaagaca cggcttgccg ggcagaatgg catatgcgcg acatccttgt
      361 tgtcgcacgg gcgttgacat gcgtactgtg ctggggcgcg gcgagcgcgg aaaaggcgcc
      421 tcaatgggcg ttcttcgtcc ctaccgaggg gtccgtctcg tcgcaggccg ccctggccgg
      481 cgatggcacc ccatggcagc cgcccagtgc aagtcgctcg tacacgagcg ctcaactccg
      541 ggaagtattg aacccgcccg actggtatcc cgacgagcac cctgccatgc ccgcaatcgt
      601 tgcggggggc tctgccggcg aggttgcagg gcgtcgcgta cgtgccgcga ggcagtgtcg
      661 ccgccggcga gacaatcgtc gtgcagggag gctcgaacac gacgatcgcc tgcgccggct
      721 gccacggcgc cagatggacc ggcgcagagg caccgcccct cgccggccgg ccgccgactt
      781 acatagtccg ccaactttgg gccttcaaga ccggcacgcg ccacgggaat tcggccgcgc
      841 cgatgcaact cgtcacagcc agaatgacaa cgaaagacat gctggaaatc gcggcgttca
      901 tggcgtcacg accttcggag tagccgaaga cggaaggagg ccgggatcag cggcgccagg
      961 agcaaggccg cccgaagcgt gagaccacac catcaagctc aacgaaacaa caaacgggat
     1021 ggcgccagag gtcacgacgt agccaccgca ggtcgccgat agcggagtac gccagccagt
     1081 tcgcggacta cctcaacggg gaccttgaag ctgcgtaggc cgcgctccat gatctcgacc
     1141 ccaatctgca ccacgacaaa cttaccgtcc tcgccgctgc ccggcgacgg cagtctcact
     1201 ccggcgcgca agcgctgtcc accctgaagg acttgctcat gaacgcccct tttcgtgccg
     1261 accaggtcgg cagcctgctg cgccccgccg agctgagccg ggcgcggcgc cgactcaagg
     1321 ccggtgagct cgatgccgac gcgctctcgg ccatcgagga tcgcgcgatc accgaagccg
     1381 tgcgccggca gcaggacatc ggcctgcaag gcatcaccga cggcgagttc cggcgcgact
     1441 ggtggcacct cgacttcctg gcccggctcg acggcgttgc gctgcaggac aacctcgggc
     1501 cgaagttcaa cgccgagggg cacgagcagc cgccgattgc cacggtcacg ggcaaggtcg
     1561 cctgcagcaa acccatcatg gtcgacgact tcgcattcct gaagtcggtc accacgggca
     1621 cgccgaagat gacgattcct tcgccatcga tgctgcatct gcgcggcggc cgcgccgcga
     1681 tctcgaagca ggcctacccc gatctggccg agttctggtc cgacgtggcc acggcctacc
     1741 gcgacgccat cggccatctg tacgacgccg gctgccgcta cctgcagctc gacgacatca
     1801 ccttcgccta cctgtgcgac gcgaagatcc agtccaattg ccgcgccaac ggcgatgatc
     1861 cggccctgct gccgcgcatc tacgccgaca ccatcaacgc ggcgctggcc ggcaagcccg
     1921 acgacctgcg cgtgaccatc cacacctgcc gcggcaactt caagagcgcg tgggtcgcgc
     1981 aaggcggcta cgcgcccgtg gtcgaggcca tgttctcgac cgacgtcgat ggctacttca
     2041 tggagttcga cagcgagcgc gccggcggct tcgagccgct gcacgtgctg caggacagcg
     2101 gcgacaaggc caagaaggtg gtgctggggc tggtcaccac caaggtcggc gcgctggagt
     2161 cgaaggacga gctgaaacgg cgcatcgacg aagccgccag ggcgctgccg ctcgaacgcc
     2221 tgtgcctgtc gccgcaatgc ggcttttcca gtacccacca cggcaatgcc ctgtcgcccg
     2281 acgagcagtg gcacaagctc gaacgcgtgg tcgaggtggc acacgacgtg tggggctgat
     2341 caccgggacg agcgacatgc aacttccatg gcgagtgctt tcctgcctgg cgacgatctt
     2401 ctgcgtcggg atggcgcacg gccaggctgc gccgggcgac ctgccgcttc tgcgacaggg
     2461 gaacttcagc cgactggagg ccgaaacggt ctccctgcag cagcggttcg cgaccggcca
     2521 ggtcaccgaa ttcgggctca ggcagttcta tcggtctttc tacgacctca ccgaacaaga
     2581 cttggcgagg ctcgacgagt ggcaggcggc ctaccccagg tcgtacgcag tgcgccttgt
     2641 tcgcggcacc tactacaagc tggccgcatt caaggcaagg ggcggcaact tcgccgccaa
     2701 cacgcctccc gaaagctttg ccgagatgga gagactgctt gcccgggcgc gacccgacct
     2761 ggaagcgtcg ttgtctctca cggaaaagcc tctcttgagc gtgtttcacc tgctggacat
     2821 gaccgacggg aatcgcgccg tgcgcaaggc gctgatggac agcggcacgc gaatgctgcc
     2881 ctcgaaccgg ctcatccgcg gccgctacat gttcgggctg acgccgcgct ggggcggctc
     2941 gtacgaggag atgaaggcgt tcctggccta cgcgaagcgc accggcgcga gcaaggtcgg
     3001 attgatggag atggaagcgc tcatgtacga cgacatggcg gaggtcgctc ttcaccaggg
     3061 cgatcaagaa agcgccgtcg tcaatttccg caaggcgctc gagctcaacg ccaaggtcgg
     3121 ccccgacggt ttcggagagc ctccgttttc caaacgctac aaatgcaggc tgccggcgct
     3181 cagggagtac tgtcgctaga acgaatcgat cgttccgact ggcgcgcgcc cgtcgcgata
     3241 tgcattggca accccggtag acgaggcttg gatccagcct agccgcgcag ccggaaccgc
     3301 tgcagcttcc cggtttgcgt tcgcggaagg tcgacgcggt aggcgattgc ccgcggatat
     3361 ttgtacggcg cgatcgt
//
LOCUS       DASRWS010000128         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2349089, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000128 DASRWS010000000
VERSION     DASRWS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349089"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /gene="flgK"
                     /locus_tag="VFF72_02310"
     CDS             <1..1914
                     /gene="flgK"
                     /locus_tag="VFF72_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZV92018.1"
                     /translation="AAAQAGLLTAGHNIANVNTPGYNRQEALFAARPGLFTGAGYMGQ
                     GVDVTTVQRIYSSFAAQRTLQSQAASTAVDAQSSSLSQLDDLFGDGTSGLNVSLDAFF
                     ASVNTVAGNAADTPSRQQLLSSAQSLVSRLQSYAQQIDTLRNGVNQQISSTIDSVNGY
                     ARQVASLNQRIATLTANGTSKDLPNDLLDQRDQLVSQINAQIGVTPVVQDDGTFNLFL
                     ANGQALVTGSAAHALVAVRADDNPQNVDIGLDIGSGVIHFRSSDLAGGALSGLLAYRD
                     TTLAAAQNQFGRVAIVLGDAFNAQHKLGVDLNGNAGGDFFTVAAPDVQPATGNTGSAV
                     VGAVIADAGALTASDYTLRYDGSNYTVTRESDGTVTSYASMPQTIDGVTISIASGAMA
                     AGDRFRIAPTRNGAADIGVAITDPARVAAAAPVTTSATATNVGTASLANAAVSSGYPA
                     APLAAPVTLTYASGTNTLSGFPAVPVTLTVNGVGTTYPAATPVPFTAGATLAFGNMSF
                     TLQGTPANGDTFTIAKNTGGTGDNRNAALLSALAGATLVGGTASLDAAYGQIVARVGT
                     DTSQANIESTAQAAILSQAQSAQQSISGVNLDEEAANLQRFQQAYQASSKVMQVAGVM
                     FNSVLDIIASATA"
     gene            1917..>2650
                     /gene="flgL"
                     /locus_tag="VFF72_02315"
     CDS             1917..>2650
                     /gene="flgL"
                     /locus_tag="VFF72_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZV92019.1"
                     /translation="MRVSTSQIYALGAESIGRTQADLLRTQQQIAAMRRILTPSDDPV
                     GSATAVGVRQSQAGVDRHDASIGVAKDALGQYDSTLTSIVTLLQNARTTTVNAGNGTL
                     NDADRGALATQLSQQLDQLIGLANSKDGQGNSMFAGFNVGAQAFVKTASGVVYNGDSG
                     VRSLEVGAGRSMPVSMAGDALFLANPTGNGRFVASAAPANTGGAVVGTTQAVGALDGH
                     SYTVTFNVSGGVTTYDVYDVTTAASVS"
BASE COUNT          435 a          995 c          884 g          336 t
ORIGIN      
        1 gccgccgcac aggcggggct gttgacggcc ggccacaaca tcgccaacgt caacacgccc
       61 ggctacaacc gccaggaggc gctgttcgcc gcgcgcccgg ggcttttcac cggcgcgggc
      121 tacatggggc agggcgtcga cgtcacgacg gtgcagcgga tctacagctc gttcgccgcg
      181 cagcgcacgc tgcagtcgca ggcggcgagc acggcggtcg acgcccagtc gagctcgctg
      241 tcgcagctcg acgacctctt cggcgacggc acgtcgggat tgaacgtctc gctcgacgcg
      301 ttcttcgcga gcgtcaacac cgtcgccggc aacgcggcgg acacgccgtc gcgccagcag
      361 ctcctgtcgt cggcgcaatc gctggtgtcg cgcctgcagt cgtacgcgca gcagatcgac
      421 acgctgcgca acggcgtcaa ccagcagatc tcgtcgacga tcgactcggt caacggctac
      481 gcgcgccagg tggcgtcgct caaccagcgc atcgcgacgc tgaccgccaa cggcacgagc
      541 aaggacctgc cgaacgatct cctcgaccag cgcgaccagc ttgtgtcgca gatcaacgcg
      601 cagatcggcg tcacgccggt ggtgcaggac gacggcacgt tcaacctgtt cctggcgaac
      661 gggcaggcgc tcgtcaccgg cagcgcggcg cacgcgctcg tcgcggtgcg cgccgacgac
      721 aacccgcaga acgtcgacat cggcctcgac atcggcagcg gcgtcatcca tttcaggtcg
      781 agcgacctcg ccgggggcgc gctgtcgggg ctgctcgcct atcgcgacac gacgctcgcc
      841 gccgcgcaga accagttcgg ccgcgtggcg atcgtgctgg gcgacgcgtt caacgcgcag
      901 cacaagctcg gtgtcgacct caacggcaac gccggcggcg acttcttcac cgtcgccgcg
      961 cccgacgtgc agccggcgac cggcaacaca ggcagcgccg tcgtcggggc ggtgatcgcc
     1021 gatgccggcg cgctgaccgc gagcgactac acgctgcgct acgacggcag caactacacc
     1081 gtcacgcgcg aatcggatgg cacggtgacg agctacgcgt cgatgccgca gacgatcgac
     1141 ggcgtcacga tctcgatcgc gtcgggcgcg atggcggctg gcgaccgctt ccgcatcgcg
     1201 cccacgcgca acggcgcggc ggacatcggc gtcgcgatca ccgatcccgc gcgggtcgcg
     1261 gccgcggcgc cggtcacgac gtccgccacg gcgaccaacg tcggcacggc gagcctcgcc
     1321 aacgccgccg tgtcgagcgg ctatcccgcc gcgccgctcg ccgcgcccgt gacgctgacg
     1381 tacgcgagcg gcacgaatac gctgtccggc ttcccggcgg ttcccgtcac gctgaccgtc
     1441 aatggcgtcg gcacgacgta tcccgcggcg acgcccgtgc cgttcacggc cggcgcgacg
     1501 ctcgcgttcg gcaacatgag cttcacgctg cagggcacgc ccgcgaacgg cgacacgttc
     1561 acgatcgcga agaacacggg cggcaccggc gacaaccgca acgccgcgct cctttccgcg
     1621 ctggccggcg cgacgctcgt cggcggcacg gcgtcgctcg acgccgccta cggccagatc
     1681 gtcgcgcgcg tcggcacgga caccagccag gccaacatcg agtcgaccgc gcaggcggcg
     1741 atcctctcgc aggcacagtc cgcgcagcag tcgatctccg gcgtcaacct cgacgaggag
     1801 gccgccaacc tgcagcgctt ccagcaggcg taccaggcat cgagcaaggt gatgcaggtc
     1861 gccggcgtga tgttcaacag cgtgctcgac atcatcgcga gcgccacggc ctgatcatgc
     1921 gcgtcagcac ctcccagatc tacgcgctcg gcgcggagtc gatcggccga acgcaggccg
     1981 acctcctgcg cacccagcag cagatcgcgg cgatgcgccg catcctcacg ccgtccgacg
     2041 accccgtggg ctcggcgacg gcggtgggcg tacgccagtc gcaggccggc gtcgaccggc
     2101 acgacgccag catcggcgtc gccaaggacg cgctcggcca gtacgacagc acgctcacca
     2161 gcatcgtcac gctgctgcag aacgcgcgca cgacgacggt caacgccggc aacggcaccc
     2221 tcaacgacgc cgaccgcggc gcgctcgcga cgcagctatc gcagcagctc gaccagctga
     2281 tcggcctcgc caacagcaag gacggccagg ggaactcgat gttcgccggc ttcaacgtcg
     2341 gcgcgcaggc gttcgtcaag accgccagcg gcgtcgtcta taacggcgac tcgggcgtgc
     2401 gcagcctcga ggtcggcgcc gggcgctcga tgccggtcag catggcgggc gacgcgctgt
     2461 tcctcgccaa ccccacgggc aacggccgct tcgtcgcctc ggccgcaccg gcgaacaccg
     2521 gcggcgccgt cgtcggcacc acgcaggcgg tgggtgcgct cgacgggcat tcgtacaccg
     2581 tcacgttcaa tgtcagcggc ggcgtcacga cgtacgacgt ctacgacgtc acgaccgcgg
     2641 cgagcgtgtc
//
LOCUS       DASRWS010000129         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1492169, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000129 DASRWS010000000
VERSION     DASRWS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492169"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..640
                     /gene="leuD"
                     /locus_tag="VFF72_02320"
     CDS             26..640
                     /gene="leuD"
                     /locus_tag="VFF72_02320"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZV92020.1"
                     /translation="MEKFTTLRGVAAPLPMVNIDTDMIIPKNFLKTIKRTGLKDGLFY
                     ELRWTPDGQKKDFVLDQPAYQNAKIIVAGPNFGCGSSREHAPWALLDFGIRCVISENF
                     ADIFYNNCFKNGILPIKVPKDVIAKLMDDASRGSNAVIEVDLEKQEIKGPDGGTVHFD
                     IDPFRKHCLLNGLDDIGLTMEKKSEIDDFEARQKEAQPWLHSAA"
     gene            798..1916
                     /gene="leuB"
                     /locus_tag="VFF72_02325"
     CDS             798..1916
                     /gene="leuB"
                     /locus_tag="VFF72_02325"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZV92021.1"
                     /translation="MASNRNLLVLPGDGIGPEVMNEVRKIIAWMGKKRAVGFDIKEDV
                     VGGAALDKHGVPLSDDAMKAALEADAVLFGSVGGPKWDTADFSKKPERGLLRLRKEMD
                     LFANLRPAKVFDALVDASSLKPEIVKGLDIMIIRELTSGVYFGEPRGRQTNAQGEVEA
                     FDTQRYTVGEIRRVCAVAFELARKRKNKVLSAEKANVMHTGVLWREEATKLHKEKYSD
                     VKLEHMYADALAMQLLRAPNQFDVIVTDNLFGDILSDEASMLTGSLGLLPSASLGAPD
                     ATGRRKALYEPIHGSAPDIAGKGLANPIAETLSYAMMLRYSFDLGNEADLVEKAVENV
                     LAAGYRAGDLMVGKTQGVKKVGTQEMGDAIVKELDRLA"
     gene            complement(1989..>2408)
                     /locus_tag="VFF72_02330"
     CDS             complement(1989..>2408)
                     /locus_tag="VFF72_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZV92022.1"
                     /translation="DADELTAGFVRVASSDHPVDVVSSDRHTVKPWFAGKISYSPPVR
                     DLATGGFPLLGGRVDIFDGRKVAVLVYSHNQHKVALTLWPAGSDNDAPADIAQRDGFV
                     LAQWRHGGFEMRAVSDMEPAEMSAFVKAVNTAEGPDR"
BASE COUNT          476 a          793 c          752 g          387 t
ORIGIN      
        1 ttccggcctt tcgcgagggg tttccatgga aaaattcacg acgctgcgcg gcgtcgccgc
       61 gccgctgccg atggtcaata tcgacaccga tatgatcatc ccgaagaact tcctgaaaac
      121 catcaagcgc accggcctga aagacgggct gttctacgag ttgcgctgga cgcccgatgg
      181 ccagaagaag gatttcgttc tcgatcagcc ggcctaccag aacgccaaga tcatcgtcgc
      241 cggccccaat ttcggctgcg gatcgagccg cgagcatgcg ccctgggcac tgctcgactt
      301 cggcatccgc tgcgtcatct ccgagaattt cgccgacatc ttctacaaca actgcttcaa
      361 gaacggcatc ctgccgatca aggtgccgaa ggacgtcatc gccaagctga tggacgatgc
      421 gagccgcggc tccaacgcgg tcatcgaggt cgatctcgag aagcaggaga tcaagggtcc
      481 cgacggcggc accgtccatt tcgacatcga ccccttccgc aagcattgcc tgctgaacgg
      541 cctcgacgac atcggtctta ccatggagaa gaagtcggag atcgacgact tcgaggcgcg
      601 ccagaaggaa gcccagccct ggctgcactc cgccgcctga taccaaaccc gcctcaccct
      661 gaggaggccg cccggcggcc gtctcgaagg gtggcgtcaa acgtggtgct cgtgcccatc
      721 cttcgagacg cgcttcgcgc tcctcaggat gaggtgaaac tgaaatagga accgtcctaa
      781 cccgagagaa gaagacaatg gcgtcaaacc gcaatctgct ggtgctgcct ggcgacggca
      841 tcggacccga agtcatgaac gaagtgcgca agatcatcgc ctggatgggc aagaagcgcg
      901 cggtcggctt cgacatcaag gaagacgttg tgggtggcgc ggcgctcgac aaacacggcg
      961 tgcctctctc ggacgatgcg atgaaggcgg ccctcgaggc cgacgcggtg ctgttcggct
     1021 cggtcggcgg cccgaaatgg gacaccgccg acttcagcaa gaagcccgag cgcggtctcc
     1081 tgcgcctgcg caaggagatg gacctgttcg ccaacctgcg cccggccaag gtgttcgacg
     1141 cgctggtcga tgccagctcg ctcaagcccg agatcgtcaa gggcctcgac atcatgatca
     1201 tccgcgagct gacgagcggc gtgtatttcg gcgagccgcg cggccggcag accaacgccc
     1261 agggcgaggt cgaggcgttc gatacgcagc gctacaccgt gggcgagatc cgccgcgtct
     1321 gcgccgtggc gttcgagctc gcccgcaagc gcaagaacaa ggtgctgtcg gccgagaagg
     1381 ccaacgtcat gcacaccggc gtgctgtggc gcgaggaggc gaccaagctg cacaaggaga
     1441 agtactccga cgtgaagctg gagcacatgt acgccgatgc gctcgccatg cagctcctgc
     1501 gcgcgcccaa ccagttcgac gtcatcgtca ccgacaacct gttcggtgac attctctcgg
     1561 acgaggcctc gatgctgacg ggctcgctcg gcctcttgcc ctcggcctcg ctgggcgcac
     1621 ccgatgcgac cggccggcgc aaggcactct atgaaccgat ccacggcagc gcgcccgata
     1681 tcgcgggcaa ggggctcgcc aacccgatcg ccgagacgct ctcctatgcc atgatgctgc
     1741 gttactcctt cgacctcggc aacgaagccg acctggtcga gaaggcggtc gagaacgtgc
     1801 tggccgcggg ctatcgcgcc ggcgacctga tggtcggcaa gacccagggc gtcaagaagg
     1861 tcggcaccca ggagatgggc gacgcgatcg tgaaggaact cgatcgactg gcctgatgac
     1921 tggcctgatc tcttccggac cacgggcttc gctcactctt gagcgcgcag gatgcgcggt
     1981 ccaaaagatt accgatccgg cccttcggcg gtgttgacgg ccttgacgaa ggcagacatc
     2041 tccgccggct ccatgtccga cacggcgcgc atctcgaaac cgccatgccg ccattgcgcc
     2101 agcacgaagc cgtcgcgttg cgcgatatcg gccggcgcgt cgttgtccga cccggccggc
     2161 cagagcgtca gcgccacctt gtgctgattg tggctataga ccaggaccgc caccttgcgg
     2221 ccgtcgaaga tatcgactcg gccgcccagc agcgggaaac cgccagtggc gagatcccgc
     2281 accggtggcg aatacgagat cttgccggcg aaccacggct tcaccgtgtg gcggtccgac
     2341 gacacgacat cgaccggatg gtcgctcgag gcgacgcgca cgaagcccgc cgtcaactcg
     2401 tcggcgtc
//
LOCUS       DASRWS010000130         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_50812, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000130 DASRWS010000000
VERSION     DASRWS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50812"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFF72_02335"
     CDS             complement(<1..383)
                     /locus_tag="VFF72_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92023.1"
                     /translation="MTRAVIADDERLLREQLRARLAEVWPELEIVGEAKNGAEAIELV
                     GKTEPDLVFLDIRMPGINGIEAARAIAQLPTDDADDPAGADKAWHGCEIVFITAYDQY
                     AIEAFEQGVVDYVLKPAERDRLQVTV"
     gene            complement(380..769)
                     /locus_tag="VFF72_02340"
     CDS             complement(380..769)
                     /locus_tag="VFF72_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92024.1"
                     /translation="MKTVCPWIRVLLAIAALATLALIAAAALLGSTLPWPNATFTADG
                     QTLAIGGVAGWHVALALIGVALAVLIGLVVVVLAVAFGLGVAALAVAAVVIAALAALA
                     FVLSPLLVLGWLLWRLVRPRPAAGVSA"
     gene            complement(766..2259)
                     /locus_tag="VFF72_02345"
     CDS             complement(766..2259)
                     /locus_tag="VFF72_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZV92025.1"
                     /translation="MSPPTPPLPPPPPPAPRDWTRNARWQRWVDDFVRVFHAYANWLV
                     GITWTRFILLSLLLLILASVLADLAASNGGEREEVTIAPSVPIPPKPGAPRAEPGAPP
                     PGVTIRKKDADGRQVTISIDQNGVRINPSPAQEATSAPAGAASHAAPASPAPAASSAS
                     SAASAASATNANGDGVEIRLPRGADSAQVRAAVEEARAAVVEAIRERAREAAEQARDA
                     AQEAREEAEANRKAVRTIRRIRNEGSKPPLEGLAWLFIVASTILKISYKGRIQAEVKA
                     AAATETAESEQLKRQVVEARMAAMQAQVEPHFLFNTLASIDHLIETDPPRASQMQKNL
                     IALLRASMPSMRDATPAAHNLGREMAVIRPYLEILKVRMEDRLQTGIDVPDGLLSAEF
                     PSMMIQSLVENAIKHGLEPKAEGGRLDVKAEIVHGNLAVTVADTGLGFGRAATAGTGV
                     GMANIRERLKLLYGDRASMTVADNSPSGTVVTLTVPYQAQMRQGASA"
     gene            2367..3521
                     /locus_tag="VFF72_02350"
     CDS             2367..3521
                     /locus_tag="VFF72_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HZV92026.1"
                     /translation="MPAPAHSRVDKEFVPMQFSPSGRAVAALGLALATTAPMPSLAAS
                     SAATEPPLPDNVVQCAGLRVDAERLACYDKAVAPQLRAARTATAPGAAASEPAAVAAI
                     DAERRGHASPDDKGDFLDEFWELSPKRKRGTFNFTGYQPSYFFPVHVTSGLNRHPDSP
                     AGGHAGQLPDYRNVEAKLQISIRTKLAEDVGLPDADLWFAYTQQSLWQLYSAGISRPF
                     RATDHQPEIFYVVPAPFDLPYGVKLKMTGIGLAHQSNGQSLPFSRSWNRWYVLGGLEK
                     GEFALTARYNTRIHESDGNDDNPDLTKYRGRTELLGIWTPGFYTLSALWKTNFDSRGS
                     LQLDFTTPVSRKDPKGLRWYVQLFSGYGETLIDYNFRQTSLGAGVTLFGW"
     gene            complement(3529..>5639)
                     /locus_tag="VFF72_02355"
     CDS             complement(3529..>5639)
                     /locus_tag="VFF72_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZV92027.1"
                     /translation="SGVLAVRQAIAARVNITYKILFNDAVAMTGGQPIDGTLKVPEMT
                     RELDAEGAKKIVVVTDEPEKYEVHAVQSRLAGGVSVHHRDELDAVQKELREIAGCTVL
                     IYDQTCATEKRRRRKRGTMVDPARRVVINELVCEGCGDCSVQSNCLSVEPVETEFGRK
                     RRINQNTCNKDYSCLKGFCPSFVTVEGGTLKSAKQSGAVRPRLASVATPPDPALPLAE
                     KPWGIVVAGVGGTGVITIGQLLGMAAHLEGKGVVTQDSAGLAQKGGATWSHVQIANRA
                     EAILTTKVGTAEADLVIGCDPIVAANKASLAAMRSGRTYVALNSHATPTAAFVGDPDW
                     QFPAGQCERLVLDSVGPDHVGRIDADTLAVQLVGDSIYTNPLLLGFAWQQGRVPLSHA
                     ALMRAIELNGVQVENNKTAFEWGRRAANEPQAVKALARTGQVIELVKRSAGVDELIAR
                     REEFLVDYQDAAYARRYRAFVDKVRAREAELAFSMTATRPDAARPSTKLTEAVARYLF
                     KLMAYKDEYEVARLHADPKFLERIASQFEGTMGSDYQLSYYLAPPAIAKKNDRGELQK
                     RRFGPWMLRAFRVLARLKRLRGTPFDPFGRTDERRVERALIAEYRDSIEEVLRSLGTA
                     NHALAVEIARVPEMIRGYGHVKARHLEAMRPKWDALMADWRAGPLRIVPAGSAGPLAD
                     AGPETDAPVSTQPMEFRLPS"
BASE COUNT          815 a         1973 c         1951 g          900 t
ORIGIN      
        1 acggtgactt gcaggcgatc gcgttcggcc ggcttgagca cgtaatcgac gacgccctgc
       61 tcgaatgcct cgatcgcgta ctggtcgtag gcggtgatga agacgatctc gcatccgtgc
      121 catgccttgt cggcgccggc agggtcgtcg gcgtcgtcgg tgggcagctg ggcaatggcg
      181 cgcgccgcct cgatgccgtt gatgccgggc atgcggatgt cgagaaagac gaggtcgggc
      241 tcggtcttgc cgacgagctc gatcgcctcg gcgccgttct tggcttcgcc gacgatctcg
      301 agctccggcc acacctcggc gagtcgggcg cgaagttgct cgcgaagcag ccgctcgtcg
      361 tcggcgatga cggcgcgcgt catgccgaca ctcctgccgc cggcctcggc ctgaccagcc
      421 gccagagcag ccagccgagc acgagcagcg gcgagaggac gaaggcgagc gcggccaggg
      481 cggcgatcac gaccgccgcc accgccagcg ccgccacgcc gaggccgaag gccacggcaa
      541 ggacgacgac gaccaggccg atcagcacgg ccagtgccac gccgatcagg gccagcgcaa
      601 cgtgccagcc ggcgacgccg ccgatcgcca gggtctggcc atcggcggtg aacgtggcgt
      661 tcggccaggg cagcgtcgac ccgagcagcg ccgccgccgc gatcagcgcc agggtcgcca
      721 gcgccgcgat cgcgagcagg acccgtatcc atggacagac cgtcttcatg cgcttgctcc
      781 ttgccgcatc tgcgcctggt aggggaccgt gagcgtgacc accgtcccgc tcggcgaatt
      841 gtcggcgacc gtcatcgagg cgcggtcgcc gtagagcagc ttcagccgct cgcggatgtt
      901 cgccatgccg acgccggtgc ccgccgtcgc ggcgcggccg aagccgagcc cggtgtcggc
      961 gacggtgacc gccagattgc cgtgcacgat ctcggccttc acgtcgagcc gaccgccttc
     1021 ggccttcggc tcgaggccgt gcttgatggc gttttcgacc agcgactgga tcatcatcga
     1081 cgggaactcg gcagagagca gcccgtccgg cacgtcgatg cctgtctgca gcctgtcctc
     1141 catgcgcacc ttgaggatct cgaggtaggg ccggatcacc gccatttcgc ggccgaggtt
     1201 gtgcgcggcc ggcgtcgcgt cgcgcatcga cggcatcgag gcgcgcagca gggcgatcag
     1261 gttcttctgc atctggctgg cgcgcggcgg gtcggtctcg atcaggtgat cgatcgaggc
     1321 cagcgtgttg aagaggaagt gcggctcgac ctgggcctgc atcgccgcca tgcgcgcctc
     1381 gacgacctgg cgcttgagct gctccgactc ggcggtctcg gtcgccgcag cggccttgac
     1441 ctcggcctgg atcctgccct tgtaggaaat cttgaggatc gtcgaggcga cgatgaagag
     1501 ccacgccagg ccctccagcg gcggcttgga gccctcgttg cgaatgcgcc ggatcgtccg
     1561 cacggccttg cgattggcct cggcctcttc gcgggcctcc tgcgcggcgt cgcgggcctg
     1621 ctcggcggct tcgcgcgccc gctcgcggat cgcctcgacg accgccgcgc gggcctcctc
     1681 gaccgcggcg cggacctgcg cgctgtcggc gccgcgtggc agtcggatct cgaccccgtc
     1741 gccgttcgcg ttcgtcgcgc tcgctgcgga cgccgcggac gatgcggacg atgcggccgg
     1801 cgcgggtgat gccggtgcag cgtggctcgc ggcccccgcc ggcgccgatg tcgcctcctg
     1861 cgccggagaa ggattgatgc gcacgccgtt ctggtcgatc gagatcgtca cctggcggcc
     1921 gtcggcgtcc ttcttgcgaa tggtgacgcc gggcggcggg gcaccgggct ccgcccgcgg
     1981 tgcgcccggc ttcggaggga tcggcaccga cggcgcgatc gtcacttcct cgcgctcgcc
     2041 gccgttcgac gcggccaggt cggccagcac tgacgccagg atcagcagca gcagcgacag
     2101 caggatgaag cgggtccagg tgatgccgac cagccagttg gcatacgcat ggaagacgcg
     2161 cacgaagtcg tcgacccacc gttgccagcg cgcgttgcga gtccagtcgc gaggtgcggg
     2221 cggcggcggt ggtggcaggg gaggtgtcgg tggcgacatg gggtttccgt tcgacaccac
     2281 tgtacggggc gctgtcgtgc cttgccaaca acgatgcgac ggagcggacg acgcggccga
     2341 caggatgcat aaatgtgcga cgcctcgtgc cggcgcctgc acactcgcgg gtcgacaagg
     2401 aattcgtacc gatgcagttt tctccttcag ggcgcgccgt ggcggcgctg ggcctcgcgc
     2461 ttgccacgac ggcaccgatg ccgtcgctgg ccgcatcgtc ggccgcgacc gagccgccgt
     2521 tgccggacaa cgtcgtccaa tgcgcgggct tgcgcgtcga tgcggaacgg ctggcctgct
     2581 acgacaaggc ggtggcgccg cagctgcggg ccgcgcgcac ggccacggcg ccgggcgccg
     2641 cggcgagcga gccggccgcg gttgccgcca tcgatgccga gcgtcgcggc catgcctcac
     2701 ccgacgacaa gggcgacttc ctcgacgagt tctgggaact gagcccgaag cgcaagcgtg
     2761 gcacgttcaa cttcaccggg taccagccga gctacttctt tccggtccac gtgacgagcg
     2821 gcctgaaccg tcatccggac tcaccggccg ggggccacgc gggccagctc ccggactaca
     2881 ggaacgtcga ggccaagctg cagatctcga tccgcaccaa gcttgccgaa gacgtcggcc
     2941 tgcccgatgc cgatctctgg ttcgcctaca cgcagcagtc gctctggcag ctctacagcg
     3001 cgggcatctc gcggccgttt cgcgccaccg atcaccagcc cgagatcttc tacgtggtgc
     3061 ccgcaccctt cgacctgcct tacggcgtga agctgaagat gacgggcatc ggcctggccc
     3121 accagtcgaa cggccagtcg ctgcctttct cgcgcagctg gaaccgctgg tacgtgctgg
     3181 ggggcctcga gaaaggcgag ttcgcgctga ccgcgcgcta caacacccgc attcacgaga
     3241 gcgacggcaa cgacgacaac cccgatctca cgaagtaccg gggccgcacc gagctgctcg
     3301 gcatctggac gcccggcttc tacacgctga gcgcgctgtg gaagacgaac ttcgattcac
     3361 gcggctcgct gcagctcgac ttcacgacgc cggtcagccg caaggacccg aagggcctgc
     3421 gctggtacgt gcagctcttc agcggctacg gcgaaacgct gatcgactac aacttccggc
     3481 agacctcgct cggcgccggg gtgacgctgt tcggctggtg acgcggcgtc acgacggaag
     3541 ccggaactcc atcggctgag tcgataccgg ggcatcggtc tcggggccgg catcggcgag
     3601 cggacccgcc gagccggccg gcacgatgcg cagcgggccg gcccgccagt cggccatgag
     3661 cgcgtcccac ttcgggcgca tggcctcgag atggcgtgcc ttgacgtggc cgtagccgcg
     3721 gatcatctcg ggcacgcggg cgatctcgac cgccagcgcg tggttggccg tgcccaggct
     3781 gcgcagcacc tcctcgatcg agtcccggta ctcggcgatc aacgcgcgct cgacgcgcct
     3841 ctcgtcggta cgaccgaacg gatcgaacgg cgttccgcgc aagcgcttca accgcgccag
     3901 gacgcgaaac gcacgcagca tccagggccc gaagcggcgc ttctgcagct cgccgcggtc
     3961 gttcttcttc gcgatcgccg gtggcgcgag gtagtagctc agctggtagt cgctgcccat
     4021 cgtcccctcg aactggctcg cgatccgctc gagaaacttc gggtcggcgt gcagccgggc
     4081 gacctcgtac tcgtccttgt aggccatcag cttgaacagg tagcgcgcca ccgcttccgt
     4141 gagcttggtg ctcggccggg cggcgtcggg ccgcgtcgcc gtcatggaga aggcgagctc
     4201 ggcttccctg gcgcgaacct tgtcgacgaa ggcgcggtat cgacgcgcat aggcggcgtc
     4261 ctggtagtcg accaggaatt cctcgcgccg cgcgatcagc tcgtcgacgc ccgcggagcg
     4321 cttcaccagc tcgatcacct gccccgtgcg cgccaatgcc ttgacggcct gcggctcgtt
     4381 cgcggcacgc cggccccatt cgaacgcggt cttgttgttc tcgacctgca cgccattgag
     4441 ctcgatggcc cgcatcagcg cggcgtggct cagcggcacc cgtccctgct gccaggcgaa
     4501 tccgagcagg agcggattgg tgtagatcga atcgccgacg agctgcaccg cgagcgtgtc
     4561 ggcatcgatc ctgccgacat ggtcgggacc gacgctgtcg agcaccagcc gctcgcactg
     4621 gccggccggg aactgccagt cgggatcgcc gacgaaggcg gccgtcggcg tcgcgtggct
     4681 gttgagggcg acgtaggtgc ggcccgaacg catcgccgcg agcgacgcct tgttcgccgc
     4741 gacgatcgga tcgcagccga tcacgagatc ggcctcggcc gtgccgacct tggtcgtcag
     4801 gatggcttcg gcgcgattcg cgatctgcac gtggctccac gtcgcgccgc ccttttgcgc
     4861 gaggccggcc gaatcctggg tgacgacacc cttgccctcg aggtgcgcgg ccatgccgag
     4921 cagctggccg atggtgatga cgccggtgcc gccgacaccg gcgacgacga tgccccaggg
     4981 cttttcggcg agcggcagcg ccgggtcggg cggcgtggcg acgctggcca gccggggccg
     5041 caccgcgccg ctctgcttgg cgctcttcag cgttccgccc tcgaccgtga cgaagctcgg
     5101 gcagaagccc ttcaggcacg agtagtcctt gttgcaggtg ttctggttga tgcggcgctt
     5161 gcggccgaat tcggtctcga ccggctcgac cgacaggcag ttgctctgca ccgagcagtc
     5221 gccgcagccc tcgcagacga gctcgttgat gacgacgcgt cgggccggat ccaccatcgt
     5281 gccgcgtttc ctgcgccgcc gcttctcggt cgcacaggtc tggtcgtaga tcagcacggt
     5341 gcagccggca atctcgcgca gctccttctg caccgcgtcg agctcgtcgc gatggtgcac
     5401 ggagacgccg ccggccagcc gcgactgcac cgcgtgcacc tcgtacttct cgggctcgtc
     5461 ggtgacgacg acgatcttct tcgcgccctc ggcgtcgagc tcgcgcgtca tctcgggcac
     5521 cttcagcgtg ccgtcgatcg gctggccgcc ggtcatcgcc acggcgtcgt tgaagaggat
     5581 cttgtaggtg atgttgacgc gtgccgcgat cgcctggcgc accgccagca cgcccgagt
//
LOCUS       DASRWS010000131         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4080734, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000131 DASRWS010000000
VERSION     DASRWS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4080734"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFF72_02360"
     CDS             <1..1323
                     /locus_tag="VFF72_02360"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZV92028.1"
                     /translation="TDLAWDGQSLVCECGELLAASARFADESHLLTADVDLGRVARER
                     MRQNTFGQSVALHRARLASFRHVEFDLSVPSEFPWALRREVPRFPYVPADGAKRDERC
                     AEVYSIQVSALVQRLRATGIGKVVIGVSGGLDSTHALLVCARAMDVLGRPRTDVLAYT
                     MPGFATSERTLDQARRLMQAVGCSAHEIDILPSCRQMLADIGHPHAAGEPRFDITFEN
                     VQAGERTSHLFRLANLHGGLVVGTGDLSELALGWTTYGVGDHMSHYNVNASVPKTLIK
                     HLVRWVASREATAGTRRVLEDIVATEVSPELVPGAASDVPAQSSEDVVGPYDLQDFHL
                     YQVLRFGFLPSKVAFLAWTAWHDASLGAWPAGPDVARHAYSLAEIRRHLHTFLHRFFK
                     TSQFKRSVAPNAPKVGSGGSLSPRGDWRAPSDADATAWLDELREVPEE"
     gene            1320..1967
                     /locus_tag="VFF72_02365"
     CDS             1320..1967
                     /locus_tag="VFF72_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92029.1"
                     /translation="MSPGGGIGVTGSAGGARHSPGGLRRVGLSIMARRVTLAMAASTI
                     LVAACSSLLTPGAIEVPQARLQAALGKRFPVHRRLLDSIELEIGEPRLSLQPDSDRIA
                     VDVQLAAGSGGLVRSSITGTLRASAGLRLDADDNTVRLADPRIERLAIDGLPEPWRRA
                     LDRTARPLAANLLDGQVLYTLRPKDAAALAARGVRPGEIHVTPAGIVITLVPVAR"
     gene            2013..>2671
                     /locus_tag="VFF72_02370"
     CDS             2013..>2671
                     /locus_tag="VFF72_02370"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZV92030.1"
                     /translation="MDPILLFKAALMGIVEGVTEFLPVSSTGHLILAGSLLGFTESTV
                     RSAKLFEIVIQAGAIVAVVMVYWQRVRETLAGLGTGDPRQRRFALNVLIAFLPAAVIG
                     VLFNHAIKAHLFTPEVVATTFILGGIIILWAERRPPGVVRIDSVDAMSPLDALKVGLA
                     QCLALIPGTSRSGATIIGGMLFGLSRRAATDFSFFLAIPTLIGASVYSLWKDRATLGS
                     A"
BASE COUNT          359 a          960 c          924 g          428 t
ORIGIN      
        1 accgacctcg cgtgggacgg ccagtcgctg gtctgcgagt gcggcgagct gctggcggcg
       61 tcggcgcgct tcgccgatga atcgcacctg ctgacggccg acgtcgacct cggccgcgtg
      121 gcgcgcgagc ggatgcggca gaacaccttc ggccagtcgg tcgccctgca cagggcgcgg
      181 ctcgcgtcgt ttcgccacgt cgaattcgat ttgtcggtgc cgagcgaatt tccgtgggcc
      241 ctgcgtcgtg aggtgccgcg ctttccgtac gtgccggccg acggcgcgaa gcgcgacgaa
      301 cggtgcgccg aggtgtacag catccaggtt tcggcgctgg tgcagcgact gcgcgcgacg
      361 ggcatcggca aggtcgtcat cggcgtctcc ggtggcctcg attcgacgca tgcgctgctc
      421 gtctgcgccc gggcgatgga cgtcctcggc cggccgcgga ccgacgtcct cgcatacacg
      481 atgcccgggt tcgcgacgag cgagagaacg ctcgaccagg cgcgccggct gatgcaggcg
      541 gtgggctgca gcgcccacga gatcgacatc ctgccgagct gcaggcagat gctcgcggac
      601 atcggtcatc cgcatgccgc gggcgagccg cgcttcgaca tcaccttcga gaacgtgcag
      661 gcgggcgagc ggacgagcca cctctttcgc ctggccaacc tgcacggcgg gctcgtcgtc
      721 ggcaccggcg acctgagcga gctggcgctg ggttggacga cgtacggcgt cggcgaccac
      781 atgtcgcact acaacgtcaa tgccagcgtg ccgaagacgc tgatcaagca tctggtgcgc
      841 tgggttgcaa gccgcgaagc gacggccggc acgcgccgcg ttctcgaaga catcgtcgcc
      901 accgaggtca gcccggagct cgttcccggc gcggcgagcg acgtgccggc gcagtcgtcg
      961 gaggacgtcg tcgggccgta cgacctgcag gatttccatc tctaccaggt gctgcgcttc
     1021 ggcttcctgc cgagcaaggt cgcctttctc gcctggacgg cgtggcacga cgcgtcgctc
     1081 ggcgcctggc cggccggacc cgatgtcgcg cgtcacgcct actcgctggc cgagatcagg
     1141 aggcacctgc acacgttcct gcatcgcttc ttcaagacca gccagttcaa gcgctcggtc
     1201 gcaccgaatg cgccgaaggt cggatcgggc ggctcgctgt cgccgcgtgg cgactggcgc
     1261 gcgccgagcg acgccgacgc cacggcatgg ctcgacgagc tccgagaggt gcccgaggag
     1321 tgagcccggg cggcggcatc ggcgtgaccg ggagcgccgg gggcgcgagg cactctcctg
     1381 gcggcctgcg gcgtgtcggc ttgtcgatca tggctcggcg ggtgacgctc gcgatggcgg
     1441 cctcgacgat cctcgtcgcc gcctgcagct cgctgctgac gccgggcgcg atcgaggttc
     1501 cgcaggcgcg cctgcaggcg gcgctcggca agcgctttcc ggtccatcgg cgcttgctcg
     1561 actcgatcga gctcgagatc ggcgagccgc gcctgagctt gcagcccgac agcgaccgca
     1621 tcgccgtcga tgtgcagctg gccgcgggca gtggcggcct cgtccgctcg tcgatcaccg
     1681 gcacgctgcg cgccagcgcc gggctgcgcc tcgatgccga cgacaacacg gttcgcctcg
     1741 ccgacccgcg catcgagcgc ctggccatcg acggcctgcc cgagccctgg cggcgagcgc
     1801 tcgaccgcac cgcccggccc ttggccgcca atctgctcga cggccaggtt ctttacacgc
     1861 tgcgccccaa ggacgcggcg gccctcgccg cacggggcgt gcgcccgggc gagatccacg
     1921 tgacgcccgc cggcatcgtc atcacgctcg tgcccgtggc ccgctgagcg ccagcggcgt
     1981 cgctaagatg ccgccctttg ccggagaacg ccgtggaccc gatcctgctt ttcaaggccg
     2041 ccttgatggg catcgtcgag ggtgtcaccg aattcctgcc cgtctcgagc accggccacc
     2101 tgatcctcgc cggctcgctg ctcggcttca ccgagagcac cgtgcgctcg gccaagctgt
     2161 tcgagatcgt catccaggcc ggtgcgatcg tcgccgtcgt gatggtctat tggcagcggg
     2221 tgcgcgagac gcttgccggg ctgggcaccg gcgatccgcg gcaacgacgc ttcgcgctga
     2281 acgtgctgat cgccttcctg ccggcggccg tcatcggcgt cctcttcaat cacgcgatca
     2341 aggcgcacct cttcacgccc gaggtcgtcg cgaccacctt catcctcggc ggcatcatca
     2401 tcctgtgggc cgagcggcgg ccgcccggcg tcgtgcggat cgactcggtc gacgcgatga
     2461 gcccgctcga cgcgctcaag gtcggcctgg cgcaatgcct ggcgctgatt cccgggacca
     2521 gccggtcggg cgcgaccatc atcggcggca tgctgttcgg gctcagccgc cgcgcggcca
     2581 ccgacttcag cttctttctc gccatcccga cgctgatcgg cgccagcgtc tacagcctct
     2641 ggaaggaccg tgcgacgctc ggttcggccg a
//
LOCUS       DASRWS010000132         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3225956, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000132 DASRWS010000000
VERSION     DASRWS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225956"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFF72_02375"
     CDS             complement(<1..1188)
                     /locus_tag="VFF72_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZV92031.1"
                     /translation="MTLSIRLPRADGTLDRWRLRDAPAFAAPPQRAAFDRIAYSAAHV
                     VSDPLRASDPWLAAALDWDATIAYRQRLWSLGLGVAEAMDTAQRGMGLDWPTSLELIR
                     RSLDAAKDVPGALVACGCGTDHLDASAARSVDDVIRAYEEQMAAIEALGGRLIVMASR
                     ALARVADGPADYERVYDRILAQSRQPAILHWLGDMFDPALSGYWGSRDVDEAMATALG
                     VIAANPGKVDGIKISLLDKDKEIAMRRRLPEGVRMYTGDDFNYAELIEGDGTGTAATH
                     RHSDALLGIFDAIAPAASAALAALAAGDASRFHAFLAPTVPLSRHVFQAPTRFYKTGI
                     VFLAWINGHQSHFTMVGGQQSARSILHFAEAFRLADAAGLIERPELAVQRMTHLLALH
                     GLAG"
     gene            complement(1208..2371)
                     /locus_tag="VFF72_02380"
     CDS             complement(1208..2371)
                     /locus_tag="VFF72_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZV92032.1"
                     /translation="MATQRLGLIMHGVTGRMGMNQHLIRSICAIRAQGGVSLANGDRV
                     MPDPILIGRDAGKIEALARAHGIDRWGTDLDAALANQDDAVFFDAGTTQMRPTLLARA
                     IRAGKHVYCEKPIATNLNEAVEIARLAQASGLKNGAVQDKLFLPGLRKIDMLRRAGFF
                     GRMLAARIDFGYWVFEGDLQPIQRPSWNYRKEDGGGMILDMMCHWRYVLDNLFGEVKS
                     VSCLGATHIPTRWDEAGQPYAATADDAAYATCELAGHGGETVVAQLTMSWATRVRRDD
                     LVTFSVDGTNGSAVAGLQSCRAQSRVATPRPVWNPDEKQTMNFFDQWQEIPDTQVYDN
                     GFKIQWEHFIRHVVENAPYKWTLVEGAKGVQLVEAALESWQQRRWIDVPALAV"
     gene            complement(2395..3225)
                     /locus_tag="VFF72_02385"
     CDS             complement(2395..3225)
                     /locus_tag="VFF72_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZV92033.1"
                     /translation="MKVALCNEVLGGMPLEAQCELAAKLGYDGLEIAPFTLAGSPERI
                     DAAEAGRLRGVVESSGLVVTGLHWLLVAPAGLSVTTSDAAVRRRTLEVVTRLIDLCAG
                     LGGSVLVHGSPKQREVPAGETKTVAMARLRDFLADAAEVAARAGVVYCIEPLSTRETS
                     LVNTVAEATTLVRTIGQRSLRTMIDCSAAGQSEDKSVAELIDEWLPTGLIGHIQVNDP
                     NRRAPGQGEMRFAPILAALKRNGYAGTVAVEPFDYVPDGPGAAAFAAGYLRGIRESIS
                     "
     gene            complement(3232..>4701)
                     /locus_tag="VFF72_02390"
     CDS             complement(3232..>4701)
                     /locus_tag="VFF72_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZV92034.1"
                     /translation="LGLGGALLGIIVGILPGLSATLVIALLTTITIKLPANDAILVLI
                     CSYVGALYGGSRTAILLNIPGTAANAASCADGFALAQRGEAGRAIGIATSGAFMGTLF
                     GVICLAAFTSSLAEVALSFGAYEFFWLALFGVSMSGSIVGDDPLKGWLMGALGLFMAQ
                     IGQEGLYAFDRFTFGWDQLSGGISLIPALVGAFGLAEILTTLADPIEKKLVDLKDSVL
                     PRFREVLQYWRTVLRSGVIGVTTGLLPGIGEDAGAWMSYAAAKATTREKELFGKGSID
                     GLMAAETGDMASIPGHIIPALALGIPGSAPSAVLLAAMIIHGIQPGPMLMINHPQFVY
                     EVVAMTSLASVSILIFGLFLVRPLLVVLRIRRTVLMPVIFLLCTVGAFASASRLFDVY
                     AMVVIGIGAFFLRRRGYPMAPFVLGLVLGPLLDKSLRRGLVLSDGSVAPFFTRPISLV
                     FATVTIFTILLYVPAFKRGVRRVTGGARVLLRGVFTRRA"
BASE COUNT          757 a         1546 c         1723 g          675 t
ORIGIN      
        1 gccggccaga ccgtgcaatg caagcaggtg cgtcatgcgc tgcacggcga gctcaggccg
       61 ctcgatgagg ccggccgcgt cggcaaggcg gaacgcctct gcgaagtgca ggatggagcg
      121 agcgctttgc tggccgccga ccatcgtgaa gtgcgactgg tggccgttga tccaggcgag
      181 aaagacgatg cctgtcttgt agaagcgggt cggtgcctgg aagacatggc gcgacagcgg
      241 caccgtcggc gcgaggaagg catggaagcg cgaagcgtcg ccggcagcga gggcggcgag
      301 cgcagcgctc gccgccgggg cgatggcgtc gaagatgccg agcagggcgt cgctgtgccg
      361 gtgggtcgcg gccgtgcccg tgccgtcgcc ttcgatcaac tcggcgtagt tgaagtcgtc
      421 gccggtgtac atgcgaacgc cctcgggcag gcgccgccgc atcgcgatct ccttgtcctt
      481 gtcgagcagc gagatcttga ttccgtcgac cttgccgggg ttggcggcga tgacgccgag
      541 cgccgtcgcc atcgcctcgt cgacgtcgcg gctgccccag tatccggaaa gtgccggatc
      601 gaacatgtcg cccagccaat gcaggatcgc cggctgcctc gattgcgcga ggatccggtc
      661 gtagacgcgc tcgtagtcgg ccggcccgtc ggcgacgcgg gccagggccc ggctggccat
      721 gacgatcagc ctgccgccga gcgcctcgat ggcggccatc tgctcttcgt aggcccggat
      781 cacatcgtcg acggatcggg cggccgaggc gtcgagatgg tcggtgccgc agccgcaggc
      841 aacgagggcg cccggcacgt ccttggcggc atcgaggctg cgccggatca gctcgagcga
      901 ggtcggccag tcgagcccca tgccgcgctg cgcggtgtcc atcgcttcgg cgacgccgag
      961 gccgagcgac cagagccgct gccgataggc gatggtggca tcccagtcga gcgcggcggc
     1021 gagccacggg tcgctggcgc gcagcgggtc cgagacgacg tgcgccgccg agtaggcgat
     1081 gcggtcgaag gcggcgcgtt gcggcggcgc ggcgaaggcc ggcgcgtcgc gcaagcgcca
     1141 gcggtcgagc gtgccgtcgg cacggggaag gcggatcgag agggtcatga cgttgcccgg
     1201 gcaggtgtca gacggccagc gccggcacgt cgatccagcg tcgttgctgc cacgattcga
     1261 gcgccgcctc gacgagctgc acgcccttgg caccttcgac cagcgtccac ttgtacggcg
     1321 cgttctcgac gacgtggcgg atgaagtgct cccactggat cttgaagccg ttgtcgtaga
     1381 cctgggtgtc ggggatctcc tgccattgat cgaagaagtt catcgtctgc ttctcgtccg
     1441 gattccagac cggtcgcggc gtggcgacgc gcgattgcgc ccggcacgac tgcagcccgg
     1501 cgaccgcgga tccgttggtg ccgtcgacgc tgaaggtgac gaggtcgtcg cgccgcacgc
     1561 gtgtcgccca cgacatcgtc agctgcgcca ccaccgtctc gccgccgtgg ccggcgagct
     1621 cgcaggtcgc gtaggcggcg tcgtcggccg tcgccgcgta gggctggccg gcctcgtccc
     1681 agcgcgtcgg gatgtgcgtc gcgcccaggc acgacaccga cttcacctcg ccgaacaggt
     1741 tgtcgagcac gtagcgccag tggcacatca tgtcgaggat catgccgccg ccgtcttcct
     1801 tgcgatagtt ccagctcggc cgctggatcg gctgcaggtc gccctcgaag acccagtagc
     1861 cgaagtcgat gcgcgccgcg agcatgcggc cgaagaagcc ggcgcggcgc agcatgtcga
     1921 tcttgcgcag ccccggcagg aagagcttgt cctgcaccgc gccgttcttg aggcccgagg
     1981 cctgggccag ccgcgcgatc tcgaccgcct cgttgaggtt ggtggcgatc ggcttctcgc
     2041 aatagacgtg cttgccggcg cggatcgccc gggccagcag tgtcggccgc atctgcgtcg
     2101 tgccggcgtc gaagaagacg gcgtcgtcct ggttcgccag cgccgcatcg aggtcggtgc
     2161 cccagcggtc gatgccgtgc gcccgtgcga gtgcttcgat ctttccggcg tcgcggccga
     2221 tcaggatcgg atccggcatg acgcggtcgc cgttggcgag gctcacgccg ccctgcgccc
     2281 ggatcgcgca gatcgagcgg atgagatgct ggttcatgcc catgcgtccg gtgacgccgt
     2341 gcatgatgag tccgagtcgt tgcgtggcca tgtacgtgct tggtgaagga ggagtcagct
     2401 gatcgattcg cggatgccac gcagatagcc ggcggcgaag gcggcggcgc ccgggccgtc
     2461 gggaacgtag tcgaaaggct cgacggcgac ggtgcctgcg tagccgttgc gcttcagcgc
     2521 cgccaagatc ggcgcgaagc gcatctcgcc ctggccgggt gcgcgccggt tcgggtcgtt
     2581 gacctggatg tggccgatca ggccggttgg cagccattcg tcgatcagct cggcgacgct
     2641 cttgtcctcg gattgcccgg cggcgctgca gtcgatcatc gtgcgcaggc tgcgctggcc
     2701 gatggttcga accagggtcg tcgcctcggc gacggtattg accagcgacg tctcgcgcgt
     2761 ggacaacggc tcgatgcagt agacgacacc ggcgcgtgcc gcgacctcgg cggcgtcggc
     2821 caggaagtcg cgcagccgcg ccatggcgac ggtcttcgtc tcgccggccg gcacctcgcg
     2881 ctgcttcggc gagccgtgca cgaggaccga gccgccgagc ccggcgcaaa gatcgatcaa
     2941 tcgcgtcacg acctcgagcg tccggcggcg caccgcggca tcgctcgtcg tcaccgagag
     3001 accggctggc gccacgagca gccagtgcag cccggtcacc acgagacccg acgattcgac
     3061 gacgccgcgc aggcgtcctg cctcggcggc gtcgatccgc tcgggcgacc cggcgagcgt
     3121 gaagggcgcg atctcgaggc cgtcgtagcc gagcttggcg gcgagctcgc actgcgcctc
     3181 gagcggcatg ccgccgagga cctcgttgca cagcgcaacc ttcatgttcc ttcaggcccg
     3241 gcgcgtgaac acgccgcgga ggagcacccg cgcgccgccg gtgacgcgcc tgacgccgcg
     3301 cttgaacgcc gggacgtaca gcaggatggt gaagatcgtc accgtcgcga agacgagcga
     3361 gatcggacgg gtgaagaacg gcgcgacgct gccgtcggaa aggacgaggc cgcgacgcag
     3421 gctcttgtcg aggagcgggc cgaggacgag gccgagcacg aacggcgcca tcgggtagcc
     3481 gcgccggcgc aggaagaagg cgccgatgcc gatgacgacc atggcgtaga cgtcgaagag
     3541 gcgagacgcg ctggcgaagg cgccgacggt gcagagcagg aagatcaccg gcatcaggac
     3601 cgtgcggcgg atgcgcagca cgaccagcag cgggcgcacg aggaagaggc cgaagatgag
     3661 gatgctcacc gacgccagcg aggtcatggc gacgacctcg tagacgaact gcggatggtt
     3721 gatcatcagc atcggcccgg gctggatgcc gtggatgatc atcgccgcga gcagcaccgc
     3781 cgacggcgcc gagccgggaa tgccgagggc cagcgccgga atgatgtggc cgggaatcga
     3841 cgccatgtcg ccggtctcgg cggccatcag gccatcgatc gagcccttgc cgaagagctc
     3901 cttctcgcgc gtcgtcgcct tcgccgccgc gtacgacatc caggcgccgg cgtcttcgcc
     3961 gattccgggg agaagacccg tcgtcacgcc gatgacgccg gagcgcagca ccgtgcgcca
     4021 gtactgcagc acttcgcgaa agcgcggcag caccgaatcc ttcaggtcga cgagcttctt
     4081 ttcgatcggg tcggcgagcg tcgtcaggat ctcggcaagg ccgaacgcgc cgacgagcgc
     4141 cgggatgagc gagatgccgc ccgagagctg gtcccagccg aaggtgaagc ggtcgaacgc
     4201 atagaggccc tcctggccga tctgcgccat gaagaggccg agcgcgccca tcagccagcc
     4261 cttgagcggg tcgtcgccga cgatcgagcc cgacatcgag acgccgaaga gcgccagcca
     4321 gaagaactca taggcgccga acgagagcgc cacctcggcc agcgacgacg tgaaggcggc
     4381 caggcagatg acgccgaaga gcgttcccat gaacgcaccc gaggtggcga tgccgatggc
     4441 gcgaccggcc tcgccgcgct gcgccagcgc gaagccgtcg gcgcacgagg cggcgttggc
     4501 tgcggtgccg gggatgttga ggaggatggc cgtccgggaa ccgccgtaga gcgcgccgac
     4561 gtaggagcag atcaggacga ggatcgcgtc gttggcgggc agcttgatcg tgatcgtcgt
     4621 caggagcgcg atgacgagcg tcgccgacag tcccggcagg atgccgacga tgatgccgag
     4681 cagggcgccg ccgagcccga g
//
LOCUS       DASRWS010000133         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2068365, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000133 DASRWS010000000
VERSION     DASRWS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068365"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFF72_02395"
     CDS             <1..534
                     /locus_tag="VFF72_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZV92035.1"
                     /translation="TAAYGAVNQVVNYVQFPAISIAITASILGAQAIGAGRVERLGSI
                     ARTGIWMNVVLTGSLVLVGYLFSRHLMALFITSEPVIDLAQSLLHIMLWSSVVYGFAS
                     VVSGVMRSSGTVLAPMAIAIGCIVAVEVPSAYALADQFGVKGVWMAYPITFVSMLVLQ
                     TTFYRLVWKKKKIVRIV"
     gene            complement(659..1900)
                     /locus_tag="VFF72_02400"
     CDS             complement(659..1900)
                     /locus_tag="VFF72_02400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZV92036.1"
                     /translation="MLSSMSATDVAFTMVGLMQAVLGVAWLFGSWLLGDSRRVAVHWA
                     FFAASSAVSFAFYTMALHAPLVGRAESLRAGGNIILVLALIAMQRGIRIFVGRPSASR
                     IHAVALLVTLAMSYAGLSPHGSSLRVGVISLILASLAFGMARDLYRYAGDALRLRRPW
                     LMGLPGLCAAAAFAFRGLRALFLPASVASEMTTDSALNVAAALSYLVIVLSFHATLIT
                     LVVGRLLADLRHRTRHDDLTGLLNRRAMEETVAAQIRRSLRSGEPFSVLMLDLDHFKA
                     INDRFGHAAGDQALRHVAGLLQAGVREVDAIARVGGEEFLALMPGATLAAAGPVAERV
                     RAQLAGQPMTLPAGAAVVTVSIGIAEWSDPGEDMSRLLVRADAALYQAKQDGRDRVVE
                     AEPGKRPATGGPTATLTSQGA"
     gene            complement(1988..2422)
                     /locus_tag="VFF72_02405"
     CDS             complement(1988..2422)
                     /locus_tag="VFF72_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZV92037.1"
                     /translation="MEDPDRSIARTLESYKAAVLARDVPAFMRMYDTDVRVFDAWGVW
                     SYEGAAAWQVAVEGWFTSLGTERVKVEFADVQSVVGGDFASVSAFVTYAGMSAQGEQL
                     RSMENRLTWVLRMRGHVWRIVHEHTSAPVGFEDSRAILQRSR"
     gene            2549..>3407
                     /locus_tag="VFF72_02410"
     CDS             2549..>3407
                     /locus_tag="VFF72_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92038.1"
                     /translation="MGIVDVRRGLATLLCAGLSSAPALAGDEPPATAVRPVTDSYFGT
                     AVVDPYRYLENLKDPEVQAWMTAQSTHTRGVLDRIPGRAALLERIHALSNDATRRRGF
                     VRRGQRYFYELIEPGAEQPKLFYRDGLAGEEHLLLDPATLGRDAATHYAIDYFSPSWD
                     GRRVAYGVSKGGSEASVLHVLDVASGKPLAEAIARAHDSTVTWRADNASFYYFKFDPV
                     RPDTPPSESEYNARTYLHVLGHGVDGEADAAVFGRNVSGLGVPEGQITYVVGSPQSRW
                     AVAVANHNAD"
BASE COUNT          544 a         1191 c         1153 g          519 t
ORIGIN      
        1 accgccgcct acggcgccgt caaccaggtc gtcaactacg tgcagtttcc ggcgatctcg
       61 atcgccatca cggcatcgat cctgggcgcg caggcgatcg gtgccggtcg cgtcgaacgg
      121 ctcggttcga tcgcccgcac cggcatctgg atgaacgtcg tcctgaccgg ctcgctggtg
      181 ctggtcggct acctgttctc gcggcacctg atggcgctct tcatcaccag cgagccggtc
      241 atcgatctcg cccagtcgct gctgcacatc atgctctgga gcagcgtcgt ctacggcttc
      301 gcgtcggtcg tcagcggcgt gatgcgctct tcgggcaccg tgctcgcgcc gatggccatc
      361 gccatcggct gcatcgtcgc ggtcgaggtg ccgtcggcct atgcactggc cgaccaattc
      421 ggcgtcaagg gcgtctggat ggcctacccg atcaccttcg tgtcgatgct ggtcctgcag
      481 accacgttct accgcctggt gtggaagaaa aagaagatcg tccggatcgt ctgagcgaac
      541 cgcagcgcgg gaaggcggag ccattcacct cgccggtcgc gcatcctgac ccgtcagcat
      601 cgccgccagg gcgcggctcg cccaccgcgc ccggtcggca gcggcgccaa gccgcgggtc
      661 acgcaccctg gctcgtcaac gtcgccgtcg ggccaccggt tgccggccgc tttcccggct
      721 cggcctcgac gacgcggtcg cgcccgtcct gcttggcctg gtagagcgcc gcgtcggcgc
      781 gaacgagcag gcgcgacatg tcctcgccgg gatcggacca ctcggcgatg ccgatgctca
      841 cggtgaccac tgcggcgcct gcaggcaatg tcatcggctg accggcgagt tgcgcgcgta
      901 cgcgctcggc cacgggaccg gccgccgcca gcgtcgcgcc gggcatgagc gccaggaatt
      961 cctcgccgcc gacgcgcgcg atggcgtcga cctcgcgcac gccggcctgg agcaggccgg
     1021 cgacgtggcg cagcgcctgg tcgccagcgg catggccgaa gcggtcgttg atcgccttga
     1081 agtgatcgag gtcgagcatc agcaccgaga acggctcgcc gctgcgcagg ctgcggcgga
     1141 tctgcgccgc cacggtctcc tccatggcgc gccggttgag caagccggtg agatcgtcat
     1201 ggcgcgtgcg atgccgcagg tcggcgagaa ggcggccgac gaccagggtg atcagcgtcg
     1261 cgtggaagct gaggacgatg accagatagc tcaatgcggc ggcgacattg agggcactgt
     1321 cggtcgtcat ctccgaagcg acgcttgccg gaaggaagag cgcgcgcagg ccgcgaaagg
     1381 cgaacgcggc cgcggcgcaa aggcccggca aacccatcaa ccacggccgg cgcagccgca
     1441 gggcgtcgcc ggcgtagcgg tagaggtcgc gcgccatgcc gaatgcgagc gacgcgagga
     1501 tcaacgagat cacaccgacg cgcaggctgc tgccgtgcgg cgacagcccg gcgtacgaca
     1561 tcgccagcgt caccagcagg gcgacggcat ggatgcggct tgcggacggg cggccgacga
     1621 agatccggat gccgcgctgc atggcaatca gcgcgagcac gaggatgatg ttgccgcccg
     1681 cgcgcagcga ttcggcgcgg ccgacgagcg gcgcgtgcag cgccatcgtg tagaaggcga
     1741 agctgaccgc gctgctcgct gcgaagaagg cccagtgcac cgcgacgcgg cgcgagtcgc
     1801 cgagcagcca cgagccgaac agccaggcca cgccgagcac ggcctgcatc aggccgacca
     1861 tcgtaaaggc cacgtcggtg gccgacatcg aagacagcat cgccaagctc gccgccttgc
     1921 cgaaagggtc gcgcgttata gcaccgagac ggccgggcgc gctccgatcc ggccggtcag
     1981 cgcagcttca ccgcgatcgc tgcaggatgg ctctggaatc ctcgaacccg acgggggcgg
     2041 aggtgtgttc gtgaacgatc cgccagacgt gaccgcgcat tcgcagcacc caggtcagcc
     2101 ggttttccat cgaccgcagc tgctcgccct gggccgacat gccggcatac gtgacgaacg
     2161 cgctcaccga cgcgaagtcg ccgccgacga cgctttgcac gtcggcgaat tcgaccttca
     2221 cccgttccgt tcccaacgac gtgaaccagc cctcgaccgc gacctgccat gcggcggcgc
     2281 cttcgtacga ccagacgccc caggcgtcga agacgcgcac gtcggtgtcg tacatgcgca
     2341 tgaatgccgg cacgtccctg gcgagaacgg cggccttgta ggactcgaga gtccgcgcga
     2401 tcgatcggtc gggatcttcc atggctgtct cctgaacggc ctgccgggtg tgccaggctg
     2461 cgccttggca ggtgcgatcg cgtcgaggtg cctgccgttc gtcgtgcctg atctagactc
     2521 gcgcaccctc gattccaacg ggctttccat gggcattgtc gacgttcgcc gcggcctcgc
     2581 caccttgctt tgcgccggct tgtcgagcgc accggcgctg gctggcgacg aacctccggc
     2641 cacggccgtc cgccctgtga cggacagcta tttcggcacg gcggtcgtcg acccctatcg
     2701 ctatctcgag aacctgaagg atcccgaggt ccaggcgtgg atgacggcgc aatcgacgca
     2761 cacccgcggg gtgctcgacc gcattcccgg gcgcgccgcg ctgctcgagc gcatccacgc
     2821 gctgtcgaac gacgccacgc gccggcgcgg cttcgtgcgc cgcggccagc gctatttcta
     2881 cgagctgatc gagcctggcg ccgagcagcc gaagctgttc tatcgagacg gcctggccgg
     2941 tgaagagcac ctgctgctcg atccggcgac gctcgggcgc gacgccgcca cgcactatgc
     3001 gatcgactat ttcagcccat cgtgggacgg caggcgagtg gcctacgggg tgtcgaaagg
     3061 cggctccgag gcgagcgtgc tgcatgtgct cgacgtcgcg agcggcaagc ctctcgccga
     3121 ggcgatcgct cgagcccacg attcgaccgt cacctggcga gccgacaacg catcgttcta
     3181 ctacttcaag ttcgatccgg tgcggcccga cacgccgcct tcggagtcgg agtacaacgc
     3241 tcgcacgtac cttcacgtgc tcggtcacgg cgtcgacggc gaagccgacg cggccgtgtt
     3301 cggccgcaac gtctccgggc tcggcgtgcc ggagggccag atcacctacg tcgtcggttc
     3361 accgcagtcg cgctgggcgg tcgcggtcgc gaaccacaac gccgacg
//
LOCUS       DASRWS010000134         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2929034, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000134 DASRWS010000000
VERSION     DASRWS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929034"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFF72_02415"
     CDS             <1..836
                     /locus_tag="VFF72_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZV92039.1"
                     /translation="VGESGCGKSTTGRCILRLIEPTSGEVWFDGRDVSHADKDALRAL
                     ARDMQIIFQDPYASLNPRMTVGAIVGEALIIHKLTKGRNAFESRIVELLETVGLSADH
                     MRRYPHEFSGGQRQRIGIARALAVSPRLVVCDEAVSALDVSIQAQVINLLEDLQAQLG
                     LTYIFIAHDLSVVEHISNRVAVMYLGRIVELAPARELYTQPLHPYTEALLSAVPIPDP
                     AVKRKRIPLQGDVPSPIRPPAGCHFHTRCPIARFPICSTVSPPLKESRAGHHVACHFR
                     G"
     gene            1038..2477
                     /locus_tag="VFF72_02420"
     CDS             1038..2477
                     /locus_tag="VFF72_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZV92040.1"
                     /translation="MSRFDRPLLGLACAAIAGVLAGCGGSVSGGGSGNPPGTLTLTAP
                     ANLANGLTGSIALAASVPDGSDVTSVEFQIDGVTIGTPDTSAPYTATVDASAYPSGQH
                     VLRARSSSAGGAVSPWSSALVKFGGNVTQPAGFTRNESWVTGLTNATAFAQAPDGRIF
                     VCEQGGSLRVIKNGVLLPTPFATFNNVDSTGERGLIGVALHPNFASNGYVYVYSTRKA
                     GTISHNRISRLTANGDVAAAVPELAIVDLPNLSTATNHNGGGMHFGADGKLYVGVGEN
                     ANPPQAQDLSQPFGKLLRINDDGTFPGDNPFFATQAGFGQAVWAYGLRNPYTFAVDPT
                     SGRILINDVGQDTWEEIDAGAAGANYGWPNSEGPDNVTTGITGPIFAYKHSDASPPGS
                     GPGGFFVGGAIAGGSFYPATGPFPAGYQGQYYFADYVGQFVGRLDTANGNAAYAFATL
                     SGSPVDLLVGIDGAVYVLTRSGVTRISSP"
     gene            complement(2511..>2859)
                     /locus_tag="VFF72_02425"
     CDS             complement(2511..>2859)
                     /locus_tag="VFF72_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZV92041.1"
                     /translation="WAAAAFPDRLRCIASIHGANMATDRPDSPHRMAPKIRCESYFAC
                     AEIDKWAPPPAIATLEAALQEAGTPHRLEWYPGVEHGFVFPLRAGIYDRAAAERHWER
                     LFSLFARTLGTPP"
BASE COUNT          442 a         1080 c          924 g          413 t
ORIGIN      
        1 tcgtcggcga gtcgggctgc ggcaagagca cgacggggcg ctgcatcctg cgcctgatcg
       61 agccgacctc gggcgaggtc tggttcgacg gccgcgacgt gtcgcatgcc gacaaggacg
      121 cgctgcgggc gctggcgcgc gacatgcaga tcatcttcca ggatccgtac gcctcgctca
      181 atccgcgcat gacggtcggc gccatcgtcg gcgaggcgct catcatccac aagctcacga
      241 agggccgcaa cgccttcgag tcgcgcatcg tcgagctgct cgagacggtg ggcctctcgg
      301 ccgaccacat gcgccgctat ccgcacgagt tctcgggcgg acagcggcag cggatcggca
      361 tcgcccgggc actcgcggtg agcccgcggc tcgtcgtctg cgacgaggcc gtctcggcgc
      421 tcgacgtatc gatccaggcg caggtgatca acctcctcga agacctgcag gcgcagctcg
      481 gcctgaccta catcttcatc gcccacgacc tgtcggtggt cgagcacatc agcaaccgcg
      541 tcgccgtcat gtacctcggc cgcatcgtcg agctggcgcc cgcccgggag ctctacacgc
      601 agccgctgca tccctacacc gaggcgctgc tctcggcggt gccgattccc gaccccgccg
      661 tcaagcggaa gcgcatcccg ctgcaaggcg acgtgccgag cccgattcgt ccccccgcgg
      721 gatgtcactt ccacacgcgc tgcccgatcg cccgtttccc gatctgcagc accgtctcgc
      781 ctccattgaa ggagagccgc gccggtcatc acgtcgcctg ccactttcgc ggctagcgcg
      841 ccgccgcggc ggccccgact ccgaatcgct tcgatgccgg acgcgcaggc caacgcgccg
      901 cggagcgcgc ggaagcgacc gacgcgcatc acgactactt cctgtgacac tctcgcggca
      961 accggcgcga cgatgcgcgg gtcggagcgg gcccgtcgct tgcaacgccg gcatgaactt
     1021 tcacggagga ccgagacatg agccgattcg atcgacccct tctgggcctg gcctgcgccg
     1081 ccatcgccgg cgtcctcgcc ggctgcggcg gcagcgtctc cggcggcggc tcgggcaatc
     1141 cgccgggaac gctcaccctc accgcgcccg ccaatctggc caacggcctc accggctcga
     1201 tcgcgctcgc cgccagcgtt ccggacggca gcgacgtcac cagcgtcgag ttccagatcg
     1261 acggcgtcac gatcggcacg cccgacacct cggcgccgta cacggcgacc gtcgacgcca
     1321 gcgcctatcc ctcgggacag cacgtgctgc gtgcccgatc cagcagcgcc ggcggcgcgg
     1381 tgtcgccctg gtcgagcgcg ctcgtgaagt tcggcggcaa cgtcacccag ccggcgggat
     1441 tcacgcgcaa cgaaagctgg gtgaccggcc tcaccaatgc cacggcattc gcgcaggcgc
     1501 ccgacggccg gatcttcgtc tgcgagcaag gcggcagcct gcgcgtcatc aagaacggcg
     1561 ttctcctgcc gacgcctttc gccaccttca acaacgtcga ttcgacgggc gagcgcggcc
     1621 tgatcggtgt cgccctgcac ccgaacttcg ccagcaacgg ctacgtgtac gtgtattcga
     1681 cgcgcaaggc cgggacgatc tcgcacaacc ggatcagccg cctcaccgcc aacggcgacg
     1741 tcgcggcggc cgtgcccgag ctcgcgatcg tcgacctgcc gaacctgtcg accgccacca
     1801 accacaacgg cggcggcatg cacttcggcg ccgacggcaa gctctatgtc ggcgtcggcg
     1861 agaacgccaa cccgccgcag gcgcaagacc tgagccagcc cttcggcaag ctgctgcgca
     1921 tcaacgacga cggcaccttt ccgggcgaca acccgttctt cgcgacacag gccggattcg
     1981 gccaggccgt ctgggcctac ggcctgcgca acccctacac cttcgcggtc gacccgacga
     2041 gtggccgcat tctcatcaac gacgtcggcc aggacacctg ggaagagatc gacgccggtg
     2101 ccgcgggcgc caactacggc tggccgaatt ccgaaggccc cgacaacgtc acgaccggca
     2161 tcaccggccc gatcttcgcc tacaagcaca gcgacgccag cccgcccggc tcgggccccg
     2221 ggggcttctt cgtcggcggc gcgatcgccg gcggctcgtt ctatccggcc accgggccgt
     2281 ttcccgccgg gtaccagggc cagtactact tcgccgacta cgtcggccag ttcgtcggcc
     2341 gcctcgatac cgccaacggc aacgccgcct atgccttcgc gacgctctcg ggctcgccgg
     2401 tcgacctgct ggtcggcatc gacggcgccg tctacgtgct gacgcgtagc ggcgtgacgc
     2461 ggatcagctc gccctgagct gcgcgctctt cgcccgcagc gtcacggacg tcatggcggc
     2521 gtgccgagcg ttcgagcaaa gaggctgaag agccgctccc agtgccgctc ggcggcggcg
     2581 cggtcgtaga tgcccgcgcg cagcggaaag acgaagccgt gctcgacgcc cgggtaccac
     2641 tcgaggcggt gcggcgtgcc cgcctcctgc agcgccgcct cgagcgtggc gatcgctggc
     2701 ggcggcgccc acttgtcgat ctcggcgcag gcgaagtagc tctcgcagcg gatcttcggc
     2761 gccatgcggt gcggcgaatc gggccgatcg gtggccatgt tggcaccgtg gatcgaggcg
     2821 atgcagcgca gccggtcggg gaaggccgcc gcggcccac
//
LOCUS       DASRWS010000135         7076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_343509, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000135 DASRWS010000000
VERSION     DASRWS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343509"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFF72_02430"
     CDS             <1..324
                     /locus_tag="VFF72_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204619.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HZV92042.1"
                     /translation="RSKDLIISGGYNVYPAEIEAVLNEMAGVAESAVIGVPHADFGEA
                     VVAVLVPRGAASLDVAAMTQALKDRIANFKVPKAMFVAAELPRNAMGKVQKNQLRERH
                     RALFA"
     gene            complement(469..2175)
                     /locus_tag="VFF72_02435"
     CDS             complement(469..2175)
                     /locus_tag="VFF72_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92043.1"
                     /translation="MVTTTVPARAVIKRPMTREERKVIFASSLGTVFEWYDFYLYGSL
                     AGIIAKQFFTGLDPTAAFIASLLAFAAGFLVRPAGALVFGRLGDMIGRKYTFLITIMI
                     MGLSTVAVGILPTYAQIGVAAPVILVALRLLQGLALGGEYGGAATYVAEHAPQGKRGA
                     YTSWIQTTATLGLFLSLIVIIGTRSLLGDETFDKGWGWRVPFWLSLILLVVSIWIRLS
                     LNETPAFKRMKEQGRTSKAPLTEALARWGNGKVVLLALLGLTAGQGVVWYTGQFYALF
                     FLTAQLKVDATTAQLMIGASLLLGTPFFIFFGTLSDRIGRKPIIMAGCLLAAIGFIPL
                     FHGLTAAANPELAAAQATNKVMVRSDPSQCSFQGNPVARDIDFRTSCDIAKRFLAQSS
                     VSYDNIDAPYGTSSATITIGDKSITAPEGTVANQKFDAASVAAIAAFKKEASADLAAA
                     GYPAKAKPIEFMGAAWWMTVGILWVLVILVTMVYGPIAAMLVELFPTRIRYTSMSVPY
                     HFGNGWFGGLLPTTAFAIVASTGNMYNGLWYPIVIAAMSFVIGTLFIRETKDVDIYKN
                     DR"
     gene            complement(2361..3002)
                     /locus_tag="VFF72_02440"
     CDS             complement(2361..3002)
                     /locus_tag="VFF72_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZV92044.1"
                     /translation="MKQLDFWFDPISPYAYLAFERLPEAFAGLSYSVAYRPILFAALL
                     KHHAHKGPAEIEPKRAWTFRQVHWLAHRAGIAIETPARHPFNPLALLRLAWAAAPPGL
                     TPGRHVCERILRHVWLGGGDAEEPARLSALVAELAPRVDPAADDARQALRRATDEAIA
                     RGLFGVPTLGIDDKLFWGFDALEMAAACLRGDPWFDEPHWQREGEPRQGIRRA"
     gene            complement(2999..3208)
                     /locus_tag="VFF72_02445"
     CDS             complement(2999..3208)
                     /locus_tag="VFF72_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZV92045.1"
                     /translation="MSDTPVPSPCIDVCAIDPRTGWCEGCLRDIDEIAAWGGLDDAAK
                     RRVLGRLPSRRVHRDQRDGLEKKPA"
     gene            complement(3205..4914)
                     /locus_tag="VFF72_02450"
     CDS             complement(3205..4914)
                     /locus_tag="VFF72_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZV92046.1"
                     /translation="MQVDPITLAVLQNGLMQVCNEMDLAFVRSAFSPVIAEALDRSDG
                     IYHRDTGELIAQGELGLPVFVGTMQFTVQAVIERVRRLNIPLAPGDVFIVNDPYLGGT
                     HLMDVKFVQPFFRDGALWCWLANTGHWPDTGGMVPGGFSANATEVEQEGLRLPPVKLY
                     KQGVLDEEILSIILSNIRIADQRIGDIKAQSAALATGEKRLAALLDRYGDATVDVAVV
                     ELRRRAAAQMREQIATIPDGVYDGEAFVDSDGVDFEPLRIAMRITKTGGADTARAQLS
                     FDMSGSSPPCRGPMNSVIATTRSSIYLAMKHVFPEAAINAGTFEPLSIVDPEGTFLYA
                     HYPRPVSGCAAEVSQRIAEAVFAALGKAIPDRLFGAPAGTSGNLGIGGFDPEKNRAYV
                     MYVISGGGYGGSPHGDGISNGCSTIGISKTTPIEIMEQLYPVLFEEYSLHEGSGGAGE
                     FRGGFGVNYRIRLRRGEARVSMVMDHGRAGPPGAQGGSAGGVNKVSIIRDGAVYVPPH
                     LSKDQDIAIRAGDVIAVSTPGGGGFGLPEARAPEAVARDLALGYFAASPPGRPAAAAT
                     NAS"
     gene            5018..5434
                     /locus_tag="VFF72_02455"
     CDS             5018..5434
                     /locus_tag="VFF72_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HZV92047.1"
                     /translation="MPADLCTESEVADLVDSFYARVRADAELGPVFAAHVHDWSVHLP
                     KMVDFWSSALRKTARYRGTPMPVHIALPGLSAALFGRWLTLFHETTAAQPNAALRERA
                     DLLAERVAQSLWYGYQMHREPDRLPASLPVAADSAA"
     gene            5450..5869
                     /locus_tag="VFF72_02460"
     CDS             5450..5869
                     /locus_tag="VFF72_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZV92048.1"
                     /translation="MPSGLDALIVGQLAEALVFSDPHGIIERWNAGATAVFGFDAEEA
                     IGQRLDLIIPEHLRAAHWRGFDAAIARGATRLHGRATMTRGLHKSGRKCYVEMSFALV
                     RDDDGKVLGSVAVARDVTERIEREKSAAALAARPAAS"
     gene            complement(5897..>7076)
                     /locus_tag="VFF72_02465"
     CDS             complement(5897..>7076)
                     /locus_tag="VFF72_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZV92049.1"
                     /translation="DVGLIEGAVPQVSGELELEYAMPIHVPMVDVHTIGAGGGSIASV
                     DASGLLKVGPQSAGATPGPICYGRGGTRPTITDANLVLGRLDPDRLLAVDQAVSIDAI
                     RAVLLEQVGAPLGLDANGAAAAILRIANDRMAGAIRMVSLARGLDPRDFALFAFGGAG
                     PLHAVALARELGIPKVLIPARPGLTNALGCVVADLRHDFVATVNRPLEAVAEGRVGEI
                     LEAHIAAGQALIGRENVAVERIVALHRADMQFQGQSHILPVALESTRVTTDQLRAAFT
                     AAYLRRFGVELPEIRPVLVNLHTAVIGRRKALSLATLAPPHPGASLEDARRTSRPVWF
                     EESGFVTTPVYVRERLPAAVSFVGPAIVEQLDCTTVVDPGVAARLDPIGNFVLTMPGR
                     "
BASE COUNT         1099 a         2421 c         2516 g         1040 t
ORIGIN      
        1 cgcagcaagg acctcatcat cagcggcggc tacaacgtct atccggccga gatcgaggcg
       61 gtgctcaacg agatggccgg cgtcgccgag agcgccgtca tcggcgtgcc gcacgccgat
      121 ttcggcgagg ccgtcgtcgc cgtcctggtg ccgcgcggcg ctgcctcgct cgatgtcgcg
      181 gcgatgacgc aggcgctgaa ggaccgcatc gccaacttca aggtgcccaa ggcgatgttc
      241 gtcgctgccg agctgccgcg caacgcgatg ggcaaggtgc agaagaacca gctgcgcgag
      301 cgccaccgcg cgctgttcgc ctgagggctt ccgacacggg gtcatgcacg gccgcacggg
      361 cgagccgggt cacctcgacg gcggtcgcat ctttcgctgc cgccgccgtg ttcgcggaaa
      421 gcgcgtcggc ctcgtggcga cagccggcac cccggcgacg acgccgcgtc agcgatcgtt
      481 cttgtagatg tcgacgtcct tcgtctcgcg gatgaagagc gtgccgatga cgaagctcat
      541 cgccgcgatg acgatcgggt accacaggcc gttgtacatg ttgcccgtgg acgcgacgat
      601 ggcgaatgcc gtggtcggca gcaggccgcc gaaccagccg ttgccgaagt ggtacggcac
      661 gctcatcgag gtgtagcgga tgcgcgtcgg gaagagctcg accagcatcg ccgcaatcgg
      721 cccgtagacc atggtgacga ggatcaccaa cacccagagg atgccgacgg tcatccacca
      781 cgccgcgccc atgaactcga tcggcttggc cttggccgga tagcccgctg ccgccaggtc
      841 ggcgctcgcc tctttcttga acgccgcgat cgcggcgacg ctcgccgcgt cgaacttctg
      901 gttggcgacc gtgccctcgg gcgccgtgat cgacttgtcg ccgatggtga tcgtcgccga
      961 cgacgtgccg tacggcgcgt cgatgttgtc gtagctgacc gagctttgcg cgaggaagcg
     1021 cttggcgatg tcgcacgacg tcctgaagtc gatgtcgcgc gccaccggat tgccctggaa
     1081 cgagcactgg ctcgggtcgg aacgcaccat gaccttgttc gtcgcctgtg ccgcggcgag
     1141 ctcgggattg gccgcggcgg tgaggccgtg gaagagcggg atgaagccga tcgccgcgag
     1201 caggcaaccg gccatgatga tgggcttgcg gccgatccgg tcggacagcg tgccgaagaa
     1261 gatgaagaac ggcgtgccga ggagcagcga ggcgccgatc atcagctggg cggtcgtggc
     1321 gtcgaccttc agctgcgcgg tcaggaagaa gagcgcgtag aactggccgg tgtaccagac
     1381 gacaccctgc ccggccgtca ggccgagcag cgccagaaga acgaccttgc cgttgcccca
     1441 gcgcgccagc gcttcggtca gcggcgcctt cgacgtcctg ccctgttcct tcatgcgctt
     1501 gaaggcgggc gtctcgttca ggctgagccg gatccagatc gagacgacca gcaggatcag
     1561 cgacagccag aagggcacgc gccagcccca gcccttgtcg aaggtctcgt cgccgagcag
     1621 cgagcgggtg ccgatgatga cgatcagcga gaggaagaga ccgagcgtcg ccgtcgtctg
     1681 gatccacgac gtgtaggcac cgcgcttgcc ctgcggtgcg tgctcggcga cataggtcgc
     1741 cgcgccgccg tactcgccgc cgagcgccag gccctgcaag aggcgcagcg cgaccaggat
     1801 gaccggcgcg gcgacgccga tctgggcata ggtcggcagg atgccgaccg ccaccgtcga
     1861 caggcccatg atcatgatcg tgatcaggaa cgtgtacttg cggccgatca tgtcgccgag
     1921 ccggccgaac acgagcgcgc ccgccggccg caccaggaac cccgccgcga aggcgagcag
     1981 cgaggcgatg aacgccgccg tcggatcgag gccggtgaag aactgcttgg cgatgatgcc
     2041 ggcgagcgaa ccgtacaggt agaagtcgta ccactcgaag acggtaccga ggctcgaggc
     2101 gaagatgacc tttcgctcct cgcgcgtcat cggccgcttg atgacggcac gggccggtac
     2161 ggtggtcgtg accatggcag ggctccctga ggttggactg ttcttggcgc gcacgcgcgc
     2221 cggcgatctc tgcgccggcc gatattgtca accgccgatg cgtcgatgcg cgggacgcgt
     2281 cgcctgattc gccgaggcag tgccgcgcgg cgtgcgctaa gtctcggtcg cgtcggcgat
     2341 gggaggtcga ccgcgtccgg tcaagcgcgc cggatgcctt gtcgtggctc gccctcgcgc
     2401 tgccagtgcg gctcgtcgaa ccacgggtcg ccgcgcaggc aggcggcggc catctcgagc
     2461 gcgtcgaagc cccagaacag cttgtcgtcg atgccgagcg tcggcacgcc gaagaggccg
     2521 cgggcgatcg cctcgtcggt cgcccgtcga agggcctgcc tggcatcgtc ggccgcggga
     2581 tcgacgcgtg gcgccagctc ggcgacgagc gccgaaagcc gcgccggctc ctcggcgtcg
     2641 ccgccgccga gccagacgtg gcgaagaatc cgctcgcaga catggcgtcc cggcgtcagc
     2701 cccgggggcg ccgcggccca cgccaggcgc agcagcgcca gcggattgaa cggatggcgc
     2761 gccggcgtct cgatcgcgat gccggcacga tgcgccagcc agtgcacctg ccggaaggtc
     2821 cacgcccgct tcggctcgat ctcggccggg cccttgtgcg catggtgctt gagcagcgcg
     2881 gcgaagagga tgggccggta ggcgacgctg tacgacaggc cggcgaaggc ctcgggcaac
     2941 cgctcgaacg cgaggtacgc gtagggcgag atcgggtcga accagaagtc gagttgcttc
     3001 atgccggctt cttctcgaga ccgtcgcgct ggtcacgatg cacgcgccgc gacggcaggc
     3061 ggccgaggac ccggcgcttc gccgcatcgt cgaggcctcc ccaggcggcg atctcgtcga
     3121 tgtcgcgcag gcacccctcg caccagccgg tgcgcggatc gatcgcgcac acgtcgatgc
     3181 aggggctggg caccggtgtg tcgctcacga cgcgttcgtc gccgcggcgg ccggccggcc
     3241 cggcggcgaa gcggcgaaat agccgagcgc caggtcgcgg gcgacggcct cgggggcacg
     3301 ggcctcgggc aagccgaagc cgccgccacc cggtgtcgag acggcgatga cgtcgccggc
     3361 gcggatcgcg atgtcctggt ccttcgagag atgaggcggc acgtagacgg cgccgtcgcg
     3421 aatgatgctg accttgttga cgccgccggc gctgccgcct tgggcgccgg gcggcccggc
     3481 ccggccgtgg tccatcacca tcgacacgcg cgcctcgccg cgacgcagcc ggatccggta
     3541 gttgacgccg aagccgccgc ggaactcgcc ggcgccgccc gagccttcgt gcagcgaata
     3601 ctcctcgaac agcaccgggt agagctgctc catgatctcg atcggcgtcg tcttcgagat
     3661 gccgatcgtc gagcagccgt tgctgatgcc gtcgccgtgc gggctgccgc cgtaaccgcc
     3721 gcccgagatc acgtacatca cgtaggcgcg gttcttctcg ggatcgaagc cgccgatgcc
     3781 gaggttgccg ctggtgccgg ccggcgcgcc gaacaggcga tcgggaatcg ccttgccgag
     3841 cgccgcgaac accgcctcgg cgatgcgctg gctcacctcg gcggcgcagc ccgagaccgg
     3901 ccgcgggtaa tgcgcgtaga ggaaggtgcc ttcgggatcg acgatcgaca gcggctcgaa
     3961 ggtgccggca ttgatcgccg cctcgggaaa gacatgcttc atcgccaggt agatggacga
     4021 gcgcgtcgtt gcgatgacgc tgttcatcgg cccccggcac ggcggcgacg agccactcat
     4081 gtcgaacgag agctgcgccc gggcggtgtc ggcgccgccc gtcttggtga tgcgcatggc
     4141 gatgcgcagc ggctcgaaat cgacgccgtc ggaatcgacg aacgcctcgc cgtcgtagac
     4201 gccatcgggg atcgtcgcga tctgctcgcg catctgcgcc gccgcgcggc gccgcaattc
     4261 gacgacggcg acgtcgaccg tcgcgtcgcc gtagcgatcg agcagcgccg cgaggcgctt
     4321 ctcgccggtg gccagcgccg ccgactgcgc cttgatgtcg ccgatgcgct ggtcggcgat
     4381 gcggatgttg ctgaggatga tcgagaggat ctcttcgtcg aggacgccct gcttgtagag
     4441 cttgaccggc ggcagccgca ggccttcctg ctcgacctcg gtggcattgg cggaaaagcc
     4501 gccaggcacc atgccgccgg tgtcgggcca gtggccggtg ttggcgagcc agcaccagag
     4561 cgcgccgtcg cgaaagaacg gctgcacgaa cttgacgtcc atcaggtgcg tgccgccgag
     4621 gtacgggtcg ttgacgatga agacatcgcc cggcgcgagg ggaatgttca ggcgcctgac
     4681 gcgttcgatc accgcctgca ccgtgaactg catggtcccc acgaacaccg gcaggccgag
     4741 ctcgccctgc gcgatcagct cgccggtgtc gcggtgataa atgccgtcgg agcgatcgag
     4801 cgcttcggcg atcaccgggc tgaaggcgct gcgcacgaag gccaggtcca tttcgttgca
     4861 gacctgcatc aggccgttct gcagcaccgc gagggtgatc ggatcgactt gcatgtcgcg
     4921 attgtgcggc ggcggcgcgt cccatgcggc gtgtcgtcca ggggctgaaa cgattaggta
     4981 tacttcgcat atatcttcaa aagcctgccg atccgccatg cctgccgacc tttgcaccga
     5041 aagcgaagtc gctgatctcg tcgacagttt ctacgcgcgc gtgcgcgccg acgccgagct
     5101 cggcccggtc ttcgccgcgc acgtgcacga ctggagcgtg cacctgccca agatggtcga
     5161 tttctggtcg tcggcattgc gaaagacggc gcgctatcgc ggcacgccga tgcccgtcca
     5221 catcgccttg cccgggctga gcgcggccct cttcggccgc tggctgacgc tctttcacga
     5281 aacgaccgcc gcgcagccga acgcggcgct gcgcgaacgc gccgacctgc tggccgaacg
     5341 cgttgcgcaa agcctctggt acggctacca gatgcatcgc gagcccgacc ggttgcctgc
     5401 gtcgctgccg gtcgccgccg acagcgcggc ctgaaccgtc gccgcggcca tgccttcggg
     5461 tctcgacgcg ttgatcgtcg gccagctcgc cgaggcgctc gtcttcagcg acccgcacgg
     5521 catcatcgag cgctggaacg ccggcgccac ggccgtcttc ggcttcgacg ccgaagaagc
     5581 gatcggccag cgcctcgacc tcatcattcc cgagcatttg cgcgccgcgc actggcgcgg
     5641 tttcgacgcc gccatcgccc gcggtgcgac gcggctgcac ggccgcgcga cgatgacccg
     5701 cggcctgcac aagtccggcc gcaagtgcta cgtcgagatg agcttcgcgc tggtccgcga
     5761 cgacgacggc aaggtcctcg gctcggtcgc cgtcgcgcgc gacgtcaccg agcgcatcga
     5821 gcgggaaaag agcgcggcag ccctcgccgc gagacccgcg gcttcctgat tcgcggcgcg
     5881 gcgcgcgcgg ccggactcac cgacccggca tcgtcaggac gaagttgccg atcggatcga
     5941 gccgggccgc gacgcccggg tcgacgaccg tcgtgcagtc gagctgttcg acgatcgccg
     6001 ggccgacgaa agagaccgcc gcgggcagtc gctcgcggac gtagaccggc gtcgtcacga
     6061 agccggactc ttcgaaccag acgggtcgcg acgtgcgccg cgcgtcttcg agcgatgcgc
     6121 ccggatgcgg cggcgccagc gtcgccagcg acagcgcctt gcgccggccg atcaccgccg
     6181 tgtgcaggtt gacgagcacc ggccggatct ccggcagctc gacgccgaag cggcgcaggt
     6241 aggcggcggt gaacgccgcg cgcagctggt cggtcgtgac ccgcgtcgat tcgagcgcca
     6301 ccggcaggat gtggctctgg ccctggaact gcatgtcggc ccgatgcaag gcgacgatcc
     6361 gttcgacggc gacgttctcg cggccgatga gcgcctggcc ggcggcgatg tgggcctcga
     6421 ggatctcgcc gacccggccc tcggcgacgg cctcgagcgg ccggttgacg gtggcgacga
     6481 aatcgtgtcg caggtcggcg acgacgcagc cgagcgcgtt ggtgaggccc gggcgcgccg
     6541 ggatcagcac cttcgggatg ccgagctcgc gggccagcgc cacggcgtgc agcggtccgg
     6601 cgccgccgaa cgcgaagagc gcgaagtctc gcgggtcgag accgcgcgcc aggctcacca
     6661 tgcggatcgc gccggccatc cggtcgttgg caatgcgcag gatcgccgcc gccgcaccgt
     6721 tcgcgtcgag gccaagcggt gcgccgacct gctcgagcag gacggcgcgg atggcatcga
     6781 tcgagaccgc ttggtcgacg gcgagcaggc gatccgggtc gagccgcccg agcacgaggt
     6841 tggcatcggt gatcgtcggc cgcgttccgc cgcggccgta gcagatcggc cccggcgtcg
     6901 cgccggcgct ttgcgggccg accttgagca ggcccgaggc atcgaccgag gcgatcgagc
     6961 cgccgcccgc gccgatcgtg tgcacgtcga ccatgggcac gtggatgggc atggcatatt
     7021 cgagctcgag ctcgcccgag acctgcggca cggcgccctc gatcaggccg acgtcg
//
LOCUS       DASRWS010000136         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1776973, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000136 DASRWS010000000
VERSION     DASRWS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776973"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1055)
                     /locus_tag="VFF72_02470"
     CDS             complement(150..1055)
                     /locus_tag="VFF72_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZV92050.1"
                     /translation="MLKLPHHKRYDYIPITKRQNYSWPGGKRLAFFMALNIEHFAFGA
                     GRRLDPFNGFSSAPTSRNWAWLDYGNRIGNFRFFEMLDQFKLPASILLNSAVCYEYPE
                     IVEKIKARGDDVLGHGRTNAETLQGLWEDDERRHIRECVEVITKYIGVRPTGWMGAGA
                     IESSVTLDLLKEEGFTHCLDWPVDDQPIWMRTRSGPLLSVPYPMELNDAGTQGVRHHT
                     GREFADMLVDQFEELLEASDKQPLVFSLALHGYLLGQPMRLRPLRKAIKHCVEHKHAD
                     QVWFTRAGDIAKYCIDMKPGIIAGS"
     gene            complement(1121..2053)
                     /locus_tag="VFF72_02475"
     CDS             complement(1121..2053)
                     /locus_tag="VFF72_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92051.1"
                     /translation="MTDQRSPIRVNVFPGGFNWGLYVAIDRGLFARHGIDVTIQGTPN
                     SVAQMTGFAEGAFEIAMTAVDNIVAYVEGQGEAPIGAQPEFFAFMGSDSGFLSLVGVP
                     EIARVADFAGRTLSVDALTTGYAFVLLEILRRAGLDKGAYDVRRVGGMVQRFESLRDR
                     HEDGTMLSAPYNILAKDHGLRELVKATAVLGPYQGNVAAARRSWASRNEAQVVAFIRG
                     YREAIAWLYAPAHREEAIAILMRNLPQMPRAVAEASYVELLSPADGFFRDCDISLAGM
                     RCVLELRSRYGTGAAPLTDPTKYCDLRYYRSATG"
     gene            complement(2088..3329)
                     /locus_tag="VFF72_02480"
     CDS             complement(2088..3329)
                     /locus_tag="VFF72_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92052.1"
                     /translation="MNAPLKHADVVTSAKGGTAVMAGFAIAVLISSYAINGMDRVLFP
                     LLLTDVRHEYGFGLPEAGLLSTIFTLGMALAGLPTGYLMSRFSRKTVTQIGILIYSAG
                     TIVTVMSGGFADMLAYRAATGVGEAMQLTSLLAIFSSYFSRNRAVGVGTLNYAYAFGA
                     IIGPMLGTSLLVHYGTWRVPMIVFGVLGLVVMVLVAVVVRPWFSEVDGSTQRELRAGG
                     APTLRNRNTLVLALLSVLLGLGLYGYLGMYPTFLREQLHFAPTSIGRVMSAYGLGVLV
                     SLGTGWLGDRFPARAVLSLSFLVAAGTCALLFNGPTSFAAQAAFSFVLGATFSGTIFV
                     NLAGYSVKVVTDALAGRASGIFVTSLYGSATVAGYLVGWMARLFGWTTAADVLLVLLC
                     VIAAAISLLVQSPPASKASAR"
     gene            complement(3395..>3571)
                     /locus_tag="VFF72_02485"
     CDS             complement(3395..>3571)
                     /locus_tag="VFF72_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZV92053.1"
                     /translation="WSVPEIIANLSREYELQPGDLIFTGTPAGVGPVGPGDRLEGEID
                     GLGTLRITVAREPD"
BASE COUNT          632 a         1211 c         1182 g          546 t
ORIGIN      
        1 acgcgctcac cggagtcgat ggtgagcgcg ggcgggatcg tggcgtcgag atagccccag
       61 tgaaccgtct ccggggtggc ccggagcagc ctggtctgcg agttcggccg cgcgacgctc
      121 gacatctgat cttcttcccg ccgctgccgt cagctgcccg caatgatgcc gggcttcatg
      181 tcgatgcagt acttcgcgat gtcgccggcg cgcgtgaacc agacctggtc ggcgtgcttg
      241 tgctcgacgc aatgcttgat cgccttgcgc aacggacgca gccgcatcgg ctggccgagc
      301 aggtagccgt gcagcgccag cgagaagacg agcggctgct tgtccgatgc ctccagcagc
      361 tcctcgaact gatcgaccag catgtcggcg aattcgcgac ccgtatggtg gcgcacgccc
      421 tgggtgccgg cgtcattgag ctccatcggg taaggcaccg acagcagcgg ccccgaccgc
      481 gtccgcatcc agatcggctg gtcgtccacc ggccagtcga ggcagtgggt gaagccctcc
      541 tccttgagga gatcgagcgt cacggacgac tcgatcgcgc ccgcgcccat ccatccggtc
      601 ggccgcacgc cgatgtattt ggtgatgacc tcgacgcact cgcgaatgtg ccggcgctcg
      661 tcgtcctccc acagcccctg cagggtctcg gcgttggtgc ggccgtgacc gaggacgtcg
      721 tcgccgcgcg ctttgatctt ctcgacgatc tccggatact cgtagcagac cgccgagttc
      781 agcaggatcg aggcaggcag cttgaactgg tccagcatct cgaagaaacg gaagttgccg
      841 atgcggttgc cgtaatcgag ccaggcccag ttgcgcgagg tcggcgcgct gctgaagccg
      901 ttgaagggat cgagccgccg tcccgctccg aacgcgaagt gctcgatgtt cagcgccatg
      961 aagaacgcga gccgcttgcc gcccggccag gaatagttct gccgcttggt gatcggaatg
     1021 tagtcgtagc gcttgtgatg cggcagcttg agcatcgctg gatctcctga gttggccgga
     1081 tgacctgcag attgagcggg cggacgggcg gacgtcgggt tcagccggta gccgagcgat
     1141 aatagcgcag atcacagtat ttcgtcgggt ccgtgagcgg cgccgcgccg gtgccatacc
     1201 ggctgcgcag ctcgaggacg cagcgcatgc ccgcaagact gatgtcgcag tcgcggaaga
     1261 agccgtcggc cggactgagc agctcgacgt agcttgcctc ggcgaccgcg cgcggcatct
     1321 gcggcaggtt gcgcatcagg atggcgatcg cctcctcgcg gtgcgccggc gcgtagagcc
     1381 acgcgatggc ctcgcggtag ccgcgaatga aggcgacgac ctgcgcctcg ttgcggctcg
     1441 cccatgagcg ccgcgcggct gcgacattgc cctgataggg accgagcacg gccgtcgcct
     1501 tgacgagctc tcgcagcccg tgatccttcg ccaggatgtt gtagggcgcc gacagcatcg
     1561 tgccgtcctc gtggcgatcg cgcaggctct cgaagcgctg caccatcccg ccgacccgcc
     1621 ggacgtcgta ggcgcccttg tccaatccgg cgcgccgcag gatctccaac agcacgaagg
     1681 cgtagccggt cgtcagggca tccaccgaca gcgtgcggcc ggcgaagtcc gccacgcgcg
     1741 cgatctcggg aacgccgacc agactgagaa aaccgctgtc gctgcccatg aaggcgaaga
     1801 actcgggctg cgcgccgatc ggcgcctcgc cctgcccctc cacataggcg acgatgttgt
     1861 cgaccgccgt catggcgatc tcgaatgcgc cctcggcaaa cccggtcatc tgcgcgaccg
     1921 agttcggcgt tccctggatg gtcacgtcga tcccatgccg ggcgaacagg ccgcgatcga
     1981 tcgcgacata gaggccccag ttgaagccgc cgggaaagac gttgacccga atgggcgagc
     2041 gttgatcagt cattacgtat catgccacct gcgacctgcg cgcgccccta ccgtgccgac
     2101 gccttcgacg ccggcggaga ctggacgagg agcgagatcg ccgccgcaat aacacacaga
     2161 aggaccagca gcacgtcggc ggcggtcgtc cacccgaaca gccgcgccat ccagccgacg
     2221 agatagcccg cgacggtcgc cgaaccatag aggctcgtga cgaagatgcc cgacgcccgc
     2281 ccggccagcg cgtcggtcac gaccttgacg ctgtagcccg cgagattgac gaagatcgtc
     2341 ccgctgaagg tcgcgccgag cacgaacgag aacgccgcct gggccgcgaa gctcgtcggg
     2401 ccgttgaaca gcagcgcgca cgtgccggcg gcgaccagga agctcaggct cagcacggca
     2461 cgcgccggaa accggtcgcc cagccagccg gtcccgagcg agacgagcac gccgagcccg
     2521 taggcgctca tgacgcggcc gatactggtc ggcgcgaagt gcaactgctc gcgcaggaac
     2581 gtcgggtaca tgccgaggta gccataaagc cccaggccga gcagcacgct cagcagcgcc
     2641 agcaccaacg tgttgcgatt gcgcagcgtc ggggcaccgc ccgcgcgaag ctcgcgctgg
     2701 gtgctgccgt cgacctcgct gaaccacggc cgcaccacga cggcgaccag gaccatgacc
     2761 accaggccga gaaccccgaa gacgatcatc ggcacccgcc acgtgccgta gtgcaccagc
     2821 aggctggtgc cgagcatcgg cccgatgatc gccccgaacg cgtaggcgta gttcagcgtg
     2881 ccgacgccga ccgcacgatt gcgcgagaaa tagctggaga agatcgcgag cagcgaggtg
     2941 agttgcatcg cctcgccgac gccggtcgcg gcccgatagg cgagcatgtc ggcaaagcct
     3001 cccgacatca ccgtgacgat cgtgcctgcc gaatagatca ggatgccgat ctgggtgacg
     3061 gtctttcgcg aaaaccgcga catcaggtag ccggtcggca atccggcgag cgccatgccg
     3121 agcgtgaaga tggtcgacag gaggccggcc tcgggcagtc cgaacccgta ttcgtgccgc
     3181 acgtcggtga gcagcagcgg aaagaggacg cgatccatcc cgttgatcgc ataggaggag
     3241 atcaggaccg cgatggcgaa gccggccatg acggccgtgc cgccttttgc gctcgtcacc
     3301 acatccgcgt gcttgagagg ggcattcact gaacgtttcc ttcgtgtttt tcttgaaccc
     3361 gccggcgccg ctcaccggcc cggcctcgct cccgtcaatc cggctcgcgc gcgaccgtga
     3421 tgcgcaacgt gccgagccca tcgatctcgc cttcgaggcg gtcgcccggg ccgaccggcc
     3481 cgacgcccgc cggcgtgccg gtgaagatca ggtcgccggg ctgcagctcg tattcgcgcg
     3541 acaggttcgc gatgatctcc ggcacgctcc a
//
LOCUS       DASRWS010000137         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1494844, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000137 DASRWS010000000
VERSION     DASRWS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494844"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..498
                     /locus_tag="VFF72_02490"
     CDS             112..498
                     /locus_tag="VFF72_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HZV92054.1"
                     /translation="MAAESSLTLAQHVVQAGLWLVAAIAIFGGSLQMYLGQPDTAPRL
                     DNVHRFMAGVYLGTGLICLWAGWTIRQQGDLVYLLALGVFLAGVGRLVSMRRVGLPSP
                     AAVWLGYLVPELLLPFVIAGAHYASP"
     gene            564..1019
                     /locus_tag="VFF72_02495"
     CDS             564..1019
                     /locus_tag="VFF72_02495"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HZV92055.1"
                     /translation="MLLSSALFAFLHFAAVFGIFCTVFLEWQTMSPAPSLAEARRIQV
                     CDRWFGVFALVVLVVGFLRVYYFEKGAAYYEANLFFHAKLGLFVLIGLLSIYPTVRFI
                     KWRTQTRQGQAPVVAAAEYGRIMWLLRAELLLLLGMALCASLMARGFGA"
     gene            1035..1673
                     /locus_tag="VFF72_02500"
     CDS             1035..1673
                     /locus_tag="VFF72_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZV92056.1"
                     /translation="MFPWPALAGIVAALSVGVVSPGPSFVVVARTAMVASRSDGLGVA
                     LGMGVGGLLFAVAALLGLRTLLLALPTVYLALKLAGGVYLAWLGWRIWTSAKQTLVIP
                     AGMFQVTSRAAWRKSFMLGLGTHLGNPKTAVVYASVFAAFLPATQTAAFDIALVACVV
                     AIECGWYAAVALLLAAERPRRAYLRYKRHLDRVAGGVMMALGLRLAWSAFRS"
     gene            1681..>2021
                     /locus_tag="VFF72_02505"
     CDS             1681..>2021
                     /locus_tag="VFF72_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV92057.1"
                     /translation="MTRTFISSGSAFEAQIGYSRAVVDGDWVFVSGTTGFDYATMAIA
                     EGAPEQAEQCLKNIESALRQAGAGLADIVRVRYILPETADFEPCWPVLRRYLGEARPA
                     ATMIRAGLLDPR"
BASE COUNT          246 a          698 c          671 g          406 t
ORIGIN      
        1 caatcgcgct cgccttgcgc attgcgagac cgccgaacgg ctttctcttt cccgccgccc
       61 gatcgccccg tagcatcgcc ggatccgcac ctcgacactc agggagccgc cttggccgcc
      121 gaatcttcgt tgacccttgc acagcatgtc gtccaggcgg ggctgtggct ggtcgcggcg
      181 atcgcgatct tcggcggctc gctgcagatg tatctcggcc agcccgacac cgcgccgcga
      241 ctcgacaacg tgcatcgttt catggccggc gtgtacctcg gcacggggct gatctgcctc
      301 tgggccggat ggacgatccg ccagcagggc gatctcgtct acctgctcgc gctgggtgtg
      361 ttcctcgccg gcgtcggccg gctcgtctcg atgcgacgcg tcggcctgcc gagcccggcg
      421 gccgtgtggc tcggctatct cgttccggaa ctgttgctgc cgttcgtgat cgccggcgcc
      481 cattacgcga gcccctgaag cgttctcctt tcgcgagcgc cccggacccc gcgacacgtg
      541 caacgaactc tgctctgaaa tcgatgttgc tatccagcgc cctcttcgcc tttctccatt
      601 tcgctgcggt cttcggcatc ttctgcaccg tctttctcga atggcagacg atgagccctg
      661 cccccagcct ggcggaggcg cgtcggatcc aggtctgcga ccggtggttc ggcgtcttcg
      721 ccctcgtcgt tctcgtcgtc ggcttccttc gtgtctacta cttcgagaag ggtgccgcgt
      781 actacgaggc gaatcttttc ttccacgcca agctcggact gttcgtgctg atcggcctgc
      841 tctcgatcta cccgaccgtg cgcttcatca aatggcgcac gcagacgcgg caggggcagg
      901 cccccgtcgt tgccgcggcc gaatacgggc ggatcatgtg gttgctgcgt gccgagctgc
      961 tgctgcttct gggcatggcg ctgtgcgcga gcctgatggc ccgcggcttc ggcgcctgag
     1021 cgggaagtcg cgcgatgttc ccgtggcctg cccttgccgg catcgtcgcc gcgctgtcgg
     1081 tcggcgtggt gagcccgggc ccgagcttcg tggtcgttgc ccgcaccgcg atggtcgcgt
     1141 cgagatccga cggtctcggc gtggcgctcg gcatgggcgt gggcggcctt ctcttcgcgg
     1201 tcgcggcgtt gctcggcctg cggaccctgc tgctcgcttt gccgacggtc tatctggcgc
     1261 tcaagctggc aggcggcgtc tatctcgcct ggcttggctg gcggatatgg acgtcggcca
     1321 agcaaacgct ggtgatcccg gccggcatgt tccaggtgac atcgcgggcg gcgtggcgca
     1381 agtccttcat gctgggcctc ggcacgcacc tgggcaatcc gaagacggcg gtcgtctacg
     1441 cgagcgtctt cgcggccttc ctgccggcga cgcagaccgc cgccttcgac atcgccctgg
     1501 tggcgtgcgt cgtcgcgatc gagtgcggct ggtatgccgc ggtggcgttg ctgctggccg
     1561 cggagcgacc gcgtcgtgcc tatcttcgct acaagaggca tctcgaccgc gtcgccggcg
     1621 gcgtcatgat ggcgctcggg ctcaggctcg cgtggtcggc atttcgcagc taggttgcgc
     1681 atgaccagga cgttcatcag ctcaggctcg gccttcgagg cgcagatcgg ctattcgcgc
     1741 gccgtcgtcg acggcgactg ggtattcgtc tcgggaacga ccggcttcga ctacgcgacg
     1801 atggcgatcg ccgaaggcgc cccagagcag gccgagcaat gcctgaagaa catcgagagc
     1861 gcgctgcgcc aggctggcgc cgggctggcc gacatcgtcc gcgtcaggta catcctgccc
     1921 gagactgccg acttcgagcc ctgctggccc gtgctgcgcc gctacctcgg cgaagcgcgg
     1981 ccagcggcga cgatgattcg cgccggcctg ctcgacccgc g
//
LOCUS       DASRWS010000138         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2351922, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000138 DASRWS010000000
VERSION     DASRWS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351922"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="VFF72_02510"
     CDS             <1..1792
                     /locus_tag="VFF72_02510"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZV92058.1"
                     /translation="AGYYQRGIGEVALAALPAELRRLDASTLARRVSSAAKRALVDAR
                     DGKAPVAAPLLSDDQAIAGRRIDGTFATPGTVLLHGVTGSGKTEVYLRAAATALARGR
                     QALILVPEINLTPQLIARVSERFPGQRIVAMHSGLTIAQRLRHWLLAHDGSADIVLGT
                     RLAAFASMPRLGVVIVDEEHDPSYKQQDGSRYSARDLSVWRGRNEGVPVVLGSATPSL
                     ESWHRADGGSYQRLTLPTRIGSADWPDIRLVDMGRLPRAAGAAPALAPALVDAIESRV
                     ARGEQSLVFLNRRGYAPVLHCAECGWKSACPHCSAWRVFHRHDRTLRCHHCGLAEPVP
                     RACPDCGNADIAPIGRGTERLEEQLVHLFPRARVARIDADSTRRKGALEAQLGAVHAG
                     EVDVLVGTQMIAKGHDFRRIGLVAAVNPDGSLFSSDFRAPERLFSLLMQAAGRAGRDA
                     TQPGRSEMWVQTWHPAHPLYAALRAHDFAAFAAGQLDERRVAGLPPYSHLAVLRAEAR
                     SREAALAFLSAAKERALALPESGAVMVYAPVPLVVARVADVERMQMLVESTSRPALQA
                     MLQAWMPSLHSLRVERRVLRWAIDVDPLTI"
     gene            complement(1815..>2879)
                     /locus_tag="VFF72_02515"
     CDS             complement(1815..>2879)
                     /locus_tag="VFF72_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZV92059.1"
                     /translation="DTRVRGLSSHFVWTNRGKESLALDLKHGAASVILGKLLAGADVL
                     VQNLAPGAAARLGLSFEALHETHPRLVVCDISGYGDDADRPGPYRDRKAYDLLIQGES
                     GFLSVTGTADEPAKAGCSIADIAAAMYAYSGILAALIERDRTGVGRRIDVSMLESMVE
                     WMGFPLYYAHEGAPPPPRSGAAHATIYPYGPFPAGDGKSVLLGLQNEREWQVFCERVL
                     GRPELARDPRFASNAARNAARHELRSIIVSAFAASTAAAVTERLEAARIASAQVRTMA
                     DVWAHPQLQARQRWVDVETPAGPVAALLPPGRRDAADAGMGAVPRLGEHTAALLAELG
                     LAPTEIERLRGEGAIGEAIR"
BASE COUNT          407 a         1054 c         1003 g          415 t
ORIGIN      
        1 cgccggttac taccagcgcg gcatcggcga agtcgccttg gccgcgctgc cggcggagct
       61 gaggcgactc gacgcctcga ccctcgcgcg ccgggtttcg agcgccgcga agcgtgccct
      121 cgtcgacgcc cgcgacggca aggcgcccgt tgccgcgccg ctcctttccg atgatcaggc
      181 gatcgccggg agacggatcg acggcacctt cgcgacgccg ggaacggtgc ttctgcacgg
      241 cgtcaccggc agcggcaaga ccgaggtcta cctgcgcgcc gcggcgaccg ccctggcgcg
      301 tggcaggcag gcgctgatcc tcgttccgga gatcaacctg acgccgcaac tgatcgcgcg
      361 ggtgagcgag cgatttcccg gccagcggat cgtcgccatg cacagcggcc tgacgatcgc
      421 gcagcgactc cgacactggc tgctcgccca tgacgggagt gccgacatcg tcctcgggac
      481 gcggctggcc gcgtttgcat cgatgccgcg cctcggcgtg gtcatcgtcg acgaagagca
      541 cgatccttcg tacaagcagc aggacggctc ccgctattcg gcgcgcgacc tgtcggtctg
      601 gcgcggccgc aacgagggcg tgcccgtcgt cctcggctcg gcgacaccgt cgctcgagag
      661 ctggcaccgg gccgacggcg gcagctacca gcgcctgacg ctgccgacgc gcatcggcag
      721 cgccgactgg cccgacatcc gcctggtcga catgggacgc ttgccacgcg cggccggcgc
      781 cgcaccggcc ctggcgccgg cgctcgtcga tgcgatcgag tcgcgcgtcg cccgcggtga
      841 acaaagcctg gtgttcctga accggcgcgg ttacgccccc gtgctgcact gcgccgaatg
      901 cggctggaag agcgcctgtc cgcactgcag cgcctggcgc gtcttccatc gccatgaccg
      961 cacgctgcgc tgccatcact gcggcctcgc cgagccggtg ccgcgcgcct gtccggactg
     1021 cggcaacgcc gacatcgcgc cgatcgggcg tggcaccgag cgcctcgagg agcagctcgt
     1081 ccacctgttc ccgcgagccc gcgtcgcccg catcgatgcc gacagcacac ggcgaaaggg
     1141 agcgctcgag gcgcagctcg gcgcggtcca cgccggcgag gtcgacgtcc tcgtcggcac
     1201 ccagatgatc gccaagggac acgatttcag acgcatcggc ctggtcgccg cggtcaatcc
     1261 ggacgggtcg ctcttcagca gcgatttccg cgcccccgag cgcctgttct cgctgctcat
     1321 gcaggcggcg ggacgcgccg ggcgcgatgc cacgcagccc ggacgcagcg agatgtgggt
     1381 gcagacctgg catccggcgc acccgctcta tgcagcattg cgggcgcacg acttcgccgc
     1441 cttcgcggcc ggccagctcg acgagcgtcg cgtcgccgga ctgccgccct attcgcatct
     1501 ggccgtgctg cgcgccgaag cgagaagccg cgaagcggcg ctggccttcc tgtcggcggc
     1561 caaggagcgc gccctcgcac tgcccgaaag cggcgcggtc atggtgtacg cgccggtgcc
     1621 gctcgtggtc gcgcgggtcg ccgacgtcga gcgcatgcag atgctggtcg agtcgacgtc
     1681 gcggccggcg ctgcaggcga tgctgcaggc gtggatgccc tcgctgcatt cgcttcgcgt
     1741 cgaacgcagg gtcctgcgct gggccatcga tgtggatccg ctgacgatct agcagggacg
     1801 gcggcgacgc gccttcagcg aatcgcttcg ccgatcgcgc cttcgccacg cagacgttcg
     1861 atctccgtcg gcgccaagcc gagctcggcg agaagggcgg cggtgtgctc gcccagcctc
     1921 ggcacggcgc ccatccctgc atcggccgcg tcgcgcctgc cgggcggcag cagagccgcg
     1981 accgggccgg ccggcgtctc gacatccacc catcgctggc gtgcctgcag ctgcgggtgt
     2041 gcccagacgt cggccatcgt gcggacctgc gcgctggcga tgcgcgccgc ctccagccgc
     2101 tcggtcactg ccgcggcagt cgaggccgcg aaggcgctga cgatgatcga ccgcaactcg
     2161 tgccgggctg cgttgcgtgc cgcgttcgac gcgaaccgcg ggtcgcgggc gagctcgggc
     2221 cggcccagga cccgctcgca gaaaacctgc cattcgcgct cgttctgcaa gccaagcagc
     2281 accgacttgc catcgccggc aggaaacggg ccatacggat agatggtcgc gtgggcagcg
     2341 ccgctgcgcg gcggcggcgg agcgccttcg tgcgcgtagt acaagggaaa gcccatccac
     2401 tcgaccatgc tctcgagcat cgacacgtcg attcgccggc cgaccccggt gcgatcccgt
     2461 tcgatcagcg cggcgaggat gccgctgtac gcgtacatgg cggcggcgat gtcggcgatc
     2521 gagcagccgg ccttggcggg ctcgtcggcg gttccggtca ccgagaggaa accggactca
     2581 ccctggatca gcaggtcgta ggccttcctg tcgcgatacg gacccggtcg gtcggcgtcg
     2641 tcgccatagc ccgagatgtc gcagacgacg agccgcggat gcgtttcgtg cagcgcctcg
     2701 aacgacaggc ccaaccgcgc cgccgcaccc ggcgccaggt tctgcaccag cacgtcggcc
     2761 cccgccagca gcttgccgag gatcacgctc gcggcgccgt gcttcaggtc gagcgcgagg
     2821 ctttccttgc cgcggttggt ccagacgaaa tgcgacgaca ggccgcgaac gcgcgtgtc
//
LOCUS       DASRWS010000139         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_52831, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000139 DASRWS010000000
VERSION     DASRWS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52831"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFF72_02520"
     CDS             <1..356
                     /locus_tag="VFF72_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV92060.1"
                     /translation="LDGWIGLAGPYDFFPLEPGQPARPVFHHPNYPKNAQPIDDATPT
                     SPKTFLAAPENDKVVSPERSTVAMAARLRAAGVPVEMHLYHGISHVLLAGVFARPLRG
                     LAPALDDVARWINAN"
     gene            complement(343..642)
                     /locus_tag="VFF72_02525"
     CDS             complement(343..642)
                     /locus_tag="VFF72_02525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZV92061.1"
                     /translation="MLIVTGTLAARADTIDELRTLSLEHVARSLAEPGCLEHGVAADA
                     NDSLRLVFFERWADRAALDAHFKVPATRAFARRAGELAARPGTLQVYEASEVSLR"
     gene            774..>2019
                     /locus_tag="VFF72_02530"
     CDS             774..>2019
                     /locus_tag="VFF72_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92062.1"
                     /translation="MPRLAALSFVLAAALAVATSAHADDDTATLSPVAHARVGFENIR
                     LPGQEHLGLLGTSYLVDAPLVPGLALGPAVYGGITGRRGGFFTIGGEAAWRHKISGPL
                     GVELGLYAGGGGGGGAPVGGGLMLRPHVDLLWDFGPAALGLSLSRVRFPNGNIDSTQV
                     GLVLDAINDFRHVPASQLATPVFGIGRSGFGFDRVQLVAGAYRPRAGTALTDGTAAPR
                     TIETLGVRAEQAFGPNAYWGLEANGAAQRGVAGYAEYLGLLGYEVEALRERLNVGARV
                     EFGMGGGGGVPTGGGSLVKADVYGIVRLDNQLGLALNLGYADAPNGAWRATEASAALV
                     WSLDGPAASNIAARPARTDFSVGLERYRAARKDGSERTLGADVLRVDRYLGDRLYLSG
                     EVHSALAGGAGGYTAALVGGGWN"
BASE COUNT          266 a          761 c          713 g          279 t
ORIGIN      
        1 agctcgacgg ctggatcggc ctggccggcc cgtacgactt ctttccgctc gagcccggcc
       61 agccggcgcg cccggtgttc caccatccga actatccgaa gaacgcacag ccgatcgacg
      121 acgcgacgcc gacgtcgccg aagaccttcc tcgccgcgcc cgaaaacgac aaggtcgtga
      181 gccccgagcg cagcaccgtg gcgatggcgg caaggctgcg cgccgccggc gtgcccgtcg
      241 agatgcatct ctatcacggc atctcgcacg tcctgctcgc cggcgtcttc gcgcggccgc
      301 tgcgcggact ggcgccggcc ctcgacgacg tcgcgcgctg gatcaacgca aactgacctc
      361 gctcgcttcg tagacctgca gcgttcccgg ccgtgccgcg agctcgccgg cgcgccgtgc
      421 gaaagcgcgc gtcgccggca ccttgaagtg cgcgtcgagc gccgcgcggt cggcccagcg
      481 ctcgaagaag acaaggcgca gcgaatcgtt ggcgtcggcc gcgacgccgt gctccaggca
      541 gcccggttcg gcgagcgagc gggcgacgtg ctcgaggctc agcgtgcgca gctcgtcgat
      601 cgtgtcggcg cgggcggcga gggttccggt gacgatcagc atgttcggct ccgagtcctg
      661 aagacgggct gtcgaatcct agggcctcgt cacgaacgag cgcgatcgat gccgcgggcg
      721 gcgcgcgtgg cgcggcgacg cgcggcgagc ggacacaata gccgtcgctc gccatgcctc
      781 gcctcgctgc cctttctttc gtcctcgccg cggctctcgc ggtcgccacc tccgcacatg
      841 ccgacgacga cacggcgacg ctctcgccgg tcgcccatgc gcgcgtcggc ttcgagaaca
      901 tccgtctccc cgggcaggag cacctgggcc tgctcggcac cagctatctc gtcgacgcgc
      961 cgctcgtgcc gggactggcg ctggggccgg cggtctacgg cggcatcacc ggacggcgcg
     1021 gcggcttctt cacgatcggc ggcgaagcgg cgtggcgaca caagatcagc ggcccgctcg
     1081 gtgtcgagct cggcctctac gcgggcggcg gcggcggcgg cggagcgccg gtcggcggcg
     1141 gactcatgct gcgcccgcac gtcgacctgc tctgggactt cggccccgca gcgctcggcc
     1201 tgtcgctgtc gcgcgtgcgc tttccgaacg gcaacatcga cagcacccag gtgggcctcg
     1261 tcctcgacgc gatcaacgac ttccggcacg tgccggcgtc gcaactcgca acaccggtct
     1321 tcggcatcgg ccgcagcggc ttcggcttcg accgggtgca gcttgtcgcc ggcgcctacc
     1381 ggccccgcgc cggcacggcg ctcaccgacg gcacggcagc gccgcgcacc atcgagacgc
     1441 tcggcgtgcg cgccgagcag gcgttcgggc cgaacgccta ctgggggctc gaagccaacg
     1501 gcgcggcgca gcgcggcgtc gccggctacg ccgaatatct cggcctcctc ggctacgagg
     1561 tcgaagcgct gcgcgagcgg ctcaacgtcg gagcccgtgt cgaattcggc atgggcggcg
     1621 gcggcggcgt gccgaccggc ggcggctcgc tcgtcaaggc cgacgtctac ggcatcgttc
     1681 gcctcgacaa ccagctgggc ctcgccctca acctcgggta cgccgatgcg cccaacggcg
     1741 cgtggcgggc gaccgaagcg tcggccgcgc tcgtctggtc gctcgacggg ccggcggcgt
     1801 cgaacatcgc cgcccgaccg gcacgcaccg atttcagcgt cggcctcgag cgctatcgtg
     1861 ccgcccgcaa ggacggcagc gagcgcaccc tcggcgccga cgtgctgcgg gtcgatcgct
     1921 acctcggcga ccgcctctac ctgtccggcg aggtgcacag cgccctggcg ggcggcgccg
     1981 gcggctacac cgcggcgctg gtcggcggcg gctggaacc
//
LOCUS       DASRWS010000140         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3501079, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000140 DASRWS010000000
VERSION     DASRWS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501079"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..693
                     /locus_tag="VFF72_02535"
     CDS             142..693
                     /locus_tag="VFF72_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZV92063.1"
                     /translation="MNTDIFGHQAPAAGFEAPLEMLSACHARIQDQCETLRRLLHHLA
                     SQGVDEQARQAATAVMRYFDTAAPLHHADEEVDLFPALIESMAGSDPVCLRELAQSLG
                     SEHRQLEAAWRRVRKGLQQVVEGNAKALAPADVEALATLYARHIDREEKELLPMAARL
                     LGDAELDGVGRAMRERRGIAPIA"
     gene            complement(787..1860)
                     /locus_tag="VFF72_02540"
     CDS             complement(787..1860)
                     /locus_tag="VFF72_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZV92064.1"
                     /translation="MAADDEGLFLKNCWYVAAWDHELIDGTKLARTLLEKAVVLYKGE
                     SGRVVALEDRCCHRGAPLSMGRVEGDCIRCMYHGMKFEPSGRCIQIPGQDIIPAKLGV
                     RSFPVVERDHLVWIWMGDPAKADPALIIDYEPLHDPAWRGMPAYMHYDANWVLIVDNL
                     SDFAHLAFVHTKTLGGSEEYAFQTRPLNIERLADGFRSERWHMNSDSPPFHRKVIADK
                     TAKVDRRNIGRMHVPGIFFLESMFAPAGTGAEKGNTAGTRQYRNCQFMTPETRRTTHF
                     FWNYLHNFDLDDPNIALSLCNSLREGFMEDKAIIEAQQKLFDREPDTQLLAIAADGAL
                     AHFRRVLGQRIAEERGEMLSAAA"
     gene            1963..>2567
                     /locus_tag="VFF72_02545"
     CDS             1963..>2567
                     /locus_tag="VFF72_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZV92065.1"
                     /translation="MNWDDYDVFCHVIEQAGFSAAARAMERPKSTISAAVLRLEDGLG
                     VRLLERTTRQVRLTDAGEALYHGVGPLFASLREARADALAQGETVAGTLRIAAPYEFG
                     AHHLGPVACAMMARHPRLKVRIDVEHEAVDPLERRYDIAFAMLESVLPASSLVQRRMF
                     SLERGVYASPELLARHGEPTTPQALAELPLLCGSGDSRWTF"
BASE COUNT          390 a          845 c          913 g          419 t
ORIGIN      
        1 tcagcgacgt cgtgatcatc gggagatcct cgcgatcgag agggtgggca aagggtcttg
       61 cggcgatgtg gcggaccgct gcggcctatg atgtccgacg ccggtcgcca tcgatgcgga
      121 tcggcggcga cgggcgacga catgaacacg gacatcttcg ggcatcaggc tccggcagcc
      181 ggcttcgagg cgccgctcga gatgctttcg gcgtgtcatg cgcgcatcca ggaccagtgc
      241 gagaccttgc gccgcctgct gcaccacctc gcgtcgcagg gcgtggacga gcaggcgcgc
      301 caggcggcca cggcggtgat gcgctacttc gacacggccg cgcccttgca tcacgccgac
      361 gaggaggtgg atctttttcc ggcactgatc gagtcgatgg ccggctccga cccggtgtgc
      421 ctgcgcgagc ttgcgcagtc gctgggcagc gagcatcgcc agctcgaggc cgcgtggcgg
      481 cgagtgcgca aggggttgca gcaggtggtc gaaggaaacg ccaaggcgct cgcgccggcc
      541 gacgtcgagg cgctcgccac cctctatgcg cgccacatcg accgcgagga aaaggaactg
      601 ctgccgatgg ccgcgcgcct gctcggcgac gccgagctcg acggcgtcgg ccgcgcgatg
      661 cgcgagcggc gagggatcgc gccgatcgcc tgagcgcccg cactcgcggg ccacgccgac
      721 gcttgcctgc gagcggttcg cgcgagacgc gtcgacgccg gtgcttgccg cagcatgcga
      781 ccgctctcag gccgctgcgg aaagcatctc gccgcgctcc tcggcgattc gctggccgag
      841 gacgcgacga aagtgcgcca gtgcgccgtc ggcggcgatg gccagcagct gggtgtcggg
      901 ctcgcggtcg aagagctttt gctgcgcctc gatgatcgcc ttgtcttcca tgaagccttc
      961 gcgcaggctg ttgcacagcg acagcgcgat attcgggtcg tcgaggtcga agttgtgcag
     1021 gtagttccag aagaagtgcg tcgtccgccg cgtctcgggc gtcatgaact ggcagttgcg
     1081 gtactggcgc gtcccggcgg tgttgccctt ctcggcgccc gtgccggccg gcgcgaacat
     1141 cgactcgaga aagaagatgc ccggcacgtg catgcggccg atgttgcgtc ggtcgacctt
     1201 cgccgtcttg tcggcaatga ccttcctgtg gaacggcggc gaatcgctgt tcatgtgcca
     1261 gcgctcggaa cgaaagccgt cggccaggcg ctcgatgttc agcggccggg tctggaaggc
     1321 gtactcctcc gagccgccca gcgtcttggt gtggacgaag gcgaggtggg cgaagtcgct
     1381 gaggttgtcg acgatgagca cccagttggc gtcgtagtgc atgtaggccg gcatgccgcg
     1441 ccaggccggg tcgtgcagag gctcgtagtc gatgatgagg gccgggtcgg ccttggcagg
     1501 atcgcccatc cagatccaga cgagatggtc gcgctcgacc accggaaagc tgcgcacgcc
     1561 gagcttggcg gggatgatgt cctgccccgg gatctggatg cacctgcccg acggctcgaa
     1621 cttcatgccg tggtacatgc agcggatgca gtcgccctcg acgcgtccca tcgacagcgg
     1681 cgcgccgcgg tgacagcagc ggtcctcgag cgcgacgacc ctgccgctct cgcccttgta
     1741 gaggacgacc gccttctcga gcagggtgcg ggcgagcttg gtgccgtcga tcagctcgtg
     1801 gtcccatgcg gccacgtacc agcagttctt cagaaagagg ccttcgtcgt ctgctgccat
     1861 cgtcactctc ctgcggcttc gctccagctt agcgaggcca cgggtggacg gcaatcggct
     1921 ggcgccgaac acactgtccc gaattcggga caatgaatcg tcatgaactg ggacgactac
     1981 gacgtcttct gccacgtcat cgagcaggcg gggttctcgg ccgcggcgcg agccatggag
     2041 cggcccaagt cgacgatcag cgccgcggtg ctgcgcctgg aggacgggct gggcgtgagg
     2101 ctgctcgagc gaaccacccg ccaggtgcgc ctcaccgacg ccggcgaggc gctctaccac
     2161 ggcgtcgggc cgctcttcgc gagcctgcgc gaggcgcgtg ccgacgcgtt ggcgcagggc
     2221 gagacggtgg ccgggacgct gcgaatcgcc gccccgtacg agttcggcgc gcatcacctc
     2281 gggccggtgg cctgcgcgat gatggctcgc catccccgcc tgaaggtacg gatcgacgtc
     2341 gagcacgaag ccgtcgatcc gctcgagcga cgctacgaca tcgccttcgc catgctcgaa
     2401 agcgtcttgc ccgcctcgag cctggtccag cggcgcatgt tctcgctcga acgcggcgtg
     2461 tacgcgtcgc cggaattgct ggcgcggcac ggcgagccga cgacgccgca ggcactggcc
     2521 gagctgccgc tgctttgcgg cagcggcgac agccgctgga cgttcca
//
LOCUS       DASRWS010000141         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2071881, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000141 DASRWS010000000
VERSION     DASRWS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071881"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFF72_02550"
     CDS             <1..436
                     /locus_tag="VFF72_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92066.1"
                     /translation="VGVPSDDFRKALGKDADYAFGMTAWLPSPDLKDRWFGDASKFAA
                     EYKAKYGYEPDYHAASGAADVEAMVMAMESAGSIDPKKVRDALAKIKFESLYGPIAFS
                     SLGQIDLPQVVIQVQGDQVVEVYGAKGFVKQPKYPMPAWNAR"
     gene            465..1328
                     /locus_tag="VFF72_02555"
     CDS             465..1328
                     /locus_tag="VFF72_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92067.1"
                     /translation="MDLLAQILVNGILLGGLYAVMALGLALVWGVLNIVNLAHGAFIM
                     LGAYASWYLYTGAHIDPFLGLPITAIGMFLVGYVLQRGLLNLVVRAPMFNTLLITFGL
                     EVVITYCAQIAFSADFRTINPGYAGSSWQIGPVTLPVVRGAAFVVAMVLTVGMWLFLL
                     HSRLGRAIRATAQNLIAARLYGVEPRHLYAMTFGLGIGLAGAAGGLYGTVSQINPYIG
                     ASLTAKSFAIAIIGGLDNPLGVIVGGIFLGIVESLTTLYVGPTFADVASFGILLLVLI
                     VRPSGLLGRTA"
     gene            1405..2352
                     /locus_tag="VFF72_02560"
     CDS             1405..2352
                     /locus_tag="VFF72_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92068.1"
                     /translation="MKPWHIAVAGVALIALAALPWFGSDTLIQFGINTLLLAVLAQGW
                     NIIGGYTGYASFGNSVFYGLGSYGVAIAMVQWKLPFAVGMAFGVGLAIVFACSLGAAV
                     LRLRGHYFAIATLALAEVMTAIVSNIPLAGQNVGLVLPPLNNDRLFYELALGLLVLAT
                     LTIFWLTKSRFGFGLIAIRENEEGAAVMGVNTTLYKVMAFTLAGIFSALAGGIHAYWI
                     TFLDPDSAFDISLNVKMIIMAVFGGPGTVLGPIVGAFSLSAVSEFLSSEVTNIAGLFF
                     GVVIVAAVVLMPRGLADLVRNVHKTGWRYFMQNIRAHRL"
     gene            2363..>3007
                     /locus_tag="VFF72_02565"
     CDS             2363..>3007
                     /locus_tag="VFF72_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92069.1"
                     /translation="MTTALLEVRHATRRFAGLVAVSDVSFVLEQGEILGLIGPNGAGK
                     TTLISLVSGTLAPSEGDVLFQGESITDVPAFRRARLGIGRTFQIMRPFPGLSVLDNVA
                     VGALFGRGGGQPKLARAREQARACLDFVGLGKFVDQRADELGGPGRKRLELAKALAMQ
                     PKLLLCDEVMAGLNHVEIEEVIDVIRKVREQGISVLVIEHVIKAIKSLSDRLLVL"
BASE COUNT          436 a         1049 c          982 g          540 t
ORIGIN      
        1 ggtcggcgtg ccgagcgacg acttccgcaa ggcgctcggc aaggacgccg actacgcctt
       61 cggcatgacc gcgtggctgc cgtcgcccga cctgaaggac cgctggttcg gcgatgcctc
      121 gaagttcgcg gccgaataca aggccaagta cggctacgag cccgactatc acgccgcctc
      181 gggcgccgcc gacgtcgagg cgatggtgat ggcgatggaa agcgccggca gcatcgatcc
      241 gaagaaggtt cgcgacgcgc tcgccaagat caagttcgaa agcctctacg ggccgatcgc
      301 cttctcgtcg ctcggccaga tcgacctgcc gcaggtcgtg atccaggtcc agggcgacca
      361 ggtcgtcgag gtctacggag cgaagggctt cgtcaagcag cccaagtacc cgatgccggc
      421 ctggaacgcc cgctgatacg gcgcaggcaa cgcgaggacg caccttggac ctgctggcgc
      481 agattctcgt caatggcatc ctgctgggcg gcctctatgc ggtgatggcg ctcggcctgg
      541 cgctggtgtg gggcgtgctc aacatcgtca acctcgcgca cggcgccttc atcatgctgg
      601 gcgcctacgc gtcgtggtac ctctacaccg gcgcccacat cgatcccttc ctcggcctgc
      661 cgatcacggc gatcggcatg ttcctcgtcg gctatgtgct gcagcgcggg ctgctcaacc
      721 tggtcgtgcg ggcgccgatg ttcaacacgc tgctgatcac cttcggtctc gaggtcgtga
      781 tcacgtattg cgcgcagatc gccttctcgg ccgactttcg caccatcaat ccgggctacg
      841 ccggcagcag ctggcagatc ggcccggtga cgctgccggt cgtgcgcggc gcggcgttcg
      901 tcgtcgcgat ggtcctgacc gtcggcatgt ggctcttcct gcttcattcg cggctcggcc
      961 gggcgatccg ggccaccgcc cagaacctga ttgccgcgcg cctctacggc gtcgagccga
     1021 ggcacctgta cgcgatgacg ttcgggctcg gcatcgggct ggcgggcgcc gccggcggtc
     1081 tctatggcac ggtgtcgcag atcaacccct acatcggcgc gtcgctcacc gccaagtcgt
     1141 tcgccatcgc catcatcggc ggtctcgaca atccgctcgg cgtcatcgtc ggcggcatct
     1201 tcctgggcat cgtcgagtcg ctgacgacgc tctacgtcgg cccgaccttc gccgacgtcg
     1261 ccagcttcgg catcctgctg ctggtgctga tcgtgcggcc gagcggcctt ctggggcgca
     1321 cggcatgatc gcgccgggcc gttcccgagc gctcatccgc ggagcgcgtg agcgcgcagg
     1381 ggatccaacg acgatgacgc attcatgaag ccctggcaca tcgccgtcgc cggcgtcgcc
     1441 ctcatcgcgc tcgcggcatt gccgtggttc ggcagcgaca cgctgatcca gttcggcatc
     1501 aacacattgc tgctcgcggt gctggcgcag ggctggaaca tcatcggcgg ctacaccggc
     1561 tacgcgtcgt tcggcaactc ggtcttctac ggcctcggca gctacggcgt cgccatcgcc
     1621 atggtgcagt ggaagctgcc gttcgccgtc ggcatggcgt tcggcgtcgg cctggcgatc
     1681 gtcttcgcct gctcgctcgg cgccgccgtg ctccgcctgc gcggccacta cttcgcgatc
     1741 gcgacgctgg ccctggcgga ggtgatgacg gcgatcgtct ccaacatccc gctcgccggc
     1801 cagaacgtcg gcctcgtcct gccgccgctc aacaacgacc ggctcttcta cgagctggcg
     1861 ctcggcctgc tcgtgctggc gacgctgacg atcttctggc tgacgaagag ccgcttcggc
     1921 ttcggcctga tcgccatccg cgagaacgag gagggcgccg cggtgatggg tgtcaacacg
     1981 acgctctaca aggtgatggc gttcacgctc gccggcatct tcagcgcgct ggccggaggc
     2041 atccacgcct actggatcac cttcctcgac cccgacagcg ccttcgacat cagcctcaac
     2101 gtcaagatga tcatcatggc ggtgttcggc ggcccgggca ccgtgctcgg gccgatcgtc
     2161 ggtgccttct cgctttcggc ggtgtcggag ttcctgtcga gcgaggtgac caacatcgcc
     2221 ggcctgttct tcggcgtcgt catcgtcgcc gccgtggttc tcatgccgag gggcctggcc
     2281 gatctcgtgc gcaacgtgca caagaccggc tggcgctatt tcatgcagaa catccgcgcc
     2341 caccggctgt gacgagtccg cgatgacgac ggcccttctc gaagtccggc acgcgacgcg
     2401 gcgcttcgcc ggcctcgtcg ccgtcagcga cgtgagcttc gtgctcgagc agggcgagat
     2461 cctcggcctg atcggcccga acggcgccgg caagacgacg ctgatcagcc ttgtcagcgg
     2521 caccctggca ccgagcgagg gcgacgtgct gttccagggg gagtcgatca ccgacgtgcc
     2581 cgccttccgg cgcgcccgtc tgggcatcgg ccgaaccttc cagatcatgc ggccctttcc
     2641 cggcctgtcg gtgctcgaca acgtcgccgt cggcgccctg ttcggacgtg gtggcggcca
     2701 gcccaagctc gcccgcgccc gcgagcaggc gcgggcctgc ctcgatttcg tgggcctcgg
     2761 caagttcgtc gaccagcgtg ccgacgagct cggcggaccg ggcaggaagc gcctcgagct
     2821 ggccaaggcg ctggcgatgc agcccaagct cctgctctgc gacgaggtca tggccggcct
     2881 gaaccacgtc gagatcgagg aggtgatcga cgtcatccgc aaggtccgcg agcaaggcat
     2941 cagcgtgctc gtcatcgagc acgtcatcaa ggcgatcaag agcctttccg accggctgct
     3001 ggtgctg
//
LOCUS       DASRWS010000142         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3229604, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000142 DASRWS010000000
VERSION     DASRWS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229604"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFF72_02570"
     CDS             complement(<1..977)
                     /locus_tag="VFF72_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV92070.1"
                     /translation="MNAPASLAQLQAASVEMTLDGRKVAAFEGETILTVAARAGIDIP
                     TLCFKDGYRPDGNCRACVVEVDGERTLAPSCCRNVQAGMKVQTGSERAKKSQRMVLEM
                     LLADLPDEGFKWVDGDSARPHGELSDWATREGVSVRPALRALRRQQPRADVSHPAMAV
                     NLDACIQCKRCVRACREEQVNDVIGYAWRGGESKIVFDFDDPMGASTCVACGECVQAC
                     PTGALMPKTAVGSQQVDAKVDSVCPFCGVGCLLTYNVRDNEIVSVDGRDGPANHSRLC
                     VKGRFGFDYASHPQRLTKPLIRKPGFPKSADFTVDPANPLSVFREATWD"
     gene            complement(974..>2417)
                     /locus_tag="VFF72_02575"
     CDS             complement(974..>2417)
                     /locus_tag="VFF72_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZV92071.1"
                     /translation="EMAGARDLLERLPALLGADVAVIAAPCVGRCEQAPVAVVHQFPV
                     PRADLDAVVEAVHGNRERHPQGDGGAAFAPADFAERSVSEREAAIAPAFVDYDSYVAA
                     GGYALAAAVTRGDRDAESLIAALESSGLRGLGGAGFPAGRKWRIVREQPAPRLMAVNI
                     DEGEPGTFKDRTYLERDPHRFLEGLLVAAKVVGIETCYVYLRDEYHGCRELLTAELAK
                     LERHAAFPLPRIEMRRGAGAYICGEESAMIESIEGQRGEPRLRPPYIASVGLFGRPTL
                     EHNFETLYWVRDIAERGPEWFSGFGRHGRKGLRSFSVSGRVKRPGVKLAPAGITLSEL
                     VDEYCGGMQDGHALYGYLPGGASGGILPASMADIPLDFDTLQPHGFFIGSAAVIVLGD
                     RDRARDAALNMMRFFAHESCGQCTPCRVGTEKAAHLMQAPVWDQSTLEDLNEVMIDAS
                     ICGLGQAAPNPVRSVQKHFAHEIGGRREPS"
BASE COUNT          351 a          851 c          853 g          362 t
ORIGIN      
        1 tcgtcccacg tcgcttcgcg gaacaccgac agcggattcg cgggatcgac ggtgaagtcg
       61 gcggacttcg ggaatcccgg cttgcggatc agcggcttcg tgaggcgctg cggatggctc
      121 gcgtagtcga aaccgaagcg ccccttgacg cacaaccggc tgtgattggc cggcccgtcg
      181 cggccgtcga cgctgacgat ctcgttgtcg cgcacgttgt aggtcaggag gcagccgacg
      241 ccgcagaagg ggcacaccga atcgaccttg gcgtcgacct gctgcgagcc gaccgcggtc
      301 ttgggcatca gcgcgccggt cgggcaggcc tgcacgcact cgccgcaggc cacgcacgtg
      361 ctggcgccca tcggatcgtc gaagtcgaag acgatcttgc tctcgccgcc gcgccaggcg
      421 tagccgatga cgtcgttgac ctgctcctcg cggcaggcgc gcacgcagcg cttgcactgg
      481 atgcaggcat cgaggttgac ggccatggcc ggatgcgaga catcggccct cggctgctgc
      541 cgtcgcaacg cgcgcagcgc cggccgcacg ctcacgcctt cgcgcgtcgc ccagtcgctc
      601 agctcgccgt gcggccgcgc ggagtcgccg tcgacccact tgaacccttc gtcgggcagg
      661 tcggcgagga gcatctcgag gaccatccgc tggctcttct tcgcccgctc gctgccggtc
      721 tgcaccttca tgccggcctg cacgttgcgg cagcagctcg gcgccagcgt ccgttcgcca
      781 tcgacctcga cgacgcaggc gcgacagttg ccgtcgggcc ggtagccgtc cttgaagcac
      841 agcgtcggga tgtcgatgcc ggcccgggcg gccaccgtca ggatggtctc gccttcgaag
      901 gccgcgacct tgcggccgtc gagcgtcatc tcgaccgagg cggcctgcag ttgcgcgagc
      961 gaagcgggtg cgttcatgaa ggctctcggc ggccgccgat ctcgtgggcg aaatgcttct
     1021 gaacgctgcg caccggattc ggcgcggcct gcccgaggcc gcagatcgag gcgtcgatca
     1081 tcacctcgtt caggtcttcg agcgtcgact ggtcccagac cggggcctgc atcagatgcg
     1141 cggccttttc ggtaccgacg cggcagggcg tgcactggcc gcagctctcg tgggcgaaaa
     1201 agcgcatcat gttgagcgcc gcgtcgcggg cccggtcgcg atcgccgagg acgatcaccg
     1261 ccgccgatcc gatgaagaag ccgtgcggct gcagcgtgtc gaagtcgagc gggatgtcgg
     1321 ccatgctcgc cggcaggatg ccgcccgagg caccccccgg caggtagccg tagagcgcgt
     1381 gcccgtcctg catgccgccg caatattcgt cgacgagctc gctcaacgtg atgccggccg
     1441 gggcgagctt gacgcccggg cgcttgacgc ggccgctgac gctgaagctg cgcaggccct
     1501 tcctgccgtg ccggccgaaa ccggagaacc attcggggcc gcgctcggcg atgtcgcgaa
     1561 cccagtagag cgtctcgaag ttgtgctcga gggtcggccg gccgaacagg cccaccgacg
     1621 cgatgtaggg cggccgcagg cgcggctcgc cgcgctgccc ttcgatgctc tcgatcatcg
     1681 ccgactcttc gccgcagatg taggcgccgg cgccgcgccg catctcgatg cgcggcagcg
     1741 gaaaggccgc gtggcgctcg agcttggcga gctcggccgt cagcagctcg cggcagccgt
     1801 ggtattcgtc gcgcaggtag acgtagcacg tctcgatgcc gacgaccttg gccgccacga
     1861 gcaggccctc gagaaagcgg tgcggatcgc gctcgagata ggtgcggtcc ttgaaggtgc
     1921 cgggctcgcc ttcgtcgatg ttgacggcca tcagccgggg cgccggctgc tcgcgaacga
     1981 tgcgccactt gcggccggcc gggaaaccgg cgccgccgag accgcgcagg cccgacgatt
     2041 ccagcgccgc gatgagcgac tcggcgtcgc gatcgcccct cgtcaccgcc gcggcgagcg
     2101 catagccgcc cgcggcgacg tagctgtcgt agtcgacgaa ggccggcgcg atcgcggcct
     2161 cgcgctcgct gacgctgcgc tcggcgaagt cggccggcgc gaacgcggca ccgccatcgc
     2221 cttgcggatg ccgctcgcgg ttgccgtgca cggcttcgac gacggcgtcg agatcggcac
     2281 gcggtacggg gaactgatgc accacggcga ccggcgcctg ctcgcagcgg ccgacgcaag
     2341 gcgcggcgat cacggcgacg tcggcaccga gcagcgccgg cagacgttcg agcagatcgc
     2401 gggcacccgc catctcg
//
LOCUS       DASRWS010000143         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2931709, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000143 DASRWS010000000
VERSION     DASRWS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2931709"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..2193)
                     /gene="dxs"
                     /locus_tag="VFF72_02580"
     CDS             complement(277..2193)
                     /gene="dxs"
                     /locus_tag="VFF72_02580"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZV92072.1"
                     /translation="MTGQSKTPLLDTVDVPADIRRLKPEQLRQLADELRQETISAVSV
                     TGGHLGAGLGVVELTVALHHVFDTPRDRLLWDVGHQAYPHKIVTGRRSRIRTLRQEGG
                     LSGFTKRAESEYDPFGAAHSSTSISAGLGMAVARDLAGSNNNVIAVIGDGAMSAGMAY
                     EAMNNAGHLRSRLIVILNDNDMSIAPPVGAMSAHLSRLLSGRPYVTLRNLGRSVAEAL
                     PKPLEMAARRAEEFARGFVAGGTLFDEMGFYYVGPVDGHNLDHLLPVLKNARDSKLDK
                     PILIHVVTKKGKGYPPAEASADKYHGVVKFDVVTGKQAKPVANAPAYQAVFARALIAE
                     AEASDKVVAITAAMPSGTSLDKFAERFPERTFDVGIAEQHGVTFAAGLACEGFKPFAA
                     IYSTFLQRAYDQCVHDVAIQKLPVRFAIDRAGLVGADGATHAGSFDVTYLACLPGFVV
                     MAPADELELMHMVATAAAIDDRPCSFRYPRGEGVGVDLPARGTPLEIGKGRILREGGK
                     IAILSFGTRLAECLTAAEDLGAKGLSTTVADARFCKPLDTDLIRRLAREHEVLITIEE
                     GAIGGFASHVLQHLASTGMLDHGLKVRPMVLPDRFIEQASPARQYEWAGLDAAHIVET
                     ALSALGRQVERARG"
     gene            complement(2242..>3102)
                     /locus_tag="VFF72_02585"
     CDS             complement(2242..>3102)
                     /locus_tag="VFF72_02585"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZV92073.1"
                     /translation="AAESTERTMDRLIPRGEAGEARVFEAMRYSSLGGGKRLRAFFVL
                     ASSTLFKVSETSALRVASAIEFVHAYSLIHDDLPAMDDDDLRRGKPSCHKQFDEATAI
                     LAGDALQALAFEVLAHEATHGDPAVRAALVSELARAAGAQGMVGGQMLDLLAETQPDM
                     SIGAITRLQRLKTGALISFSCTAGAILGKAAEPLRVALAAYAHDLGLAFQIVDDLLDV
                     EGDPAELGKATRKDADAGKATFVSILGLERARSQAALLAQQAAAHLEPFGTPADLLKQ
                     AARFVVTRRS"
BASE COUNT          500 a         1050 c         1076 g          476 t
ORIGIN      
        1 tcgccctgcc gagaccgagg cccagccgct cgagcgcgcc ggcgaacaga tattggccgg
       61 gcgaccaaac ggcctcgaag taactgccat cgagcgcgat gtcgtcgacg cggggaatga
      121 tgagatggtt gaacgccgcg ccgcgcacca tcgaatccca gatgaggaag ccgtaggcgc
      181 tgtcggagta catttccggc acgacaacga acgcggcgat ggaataaacg gcgacgagcg
      241 ccgcgacgcc ggcgaagagc agtctcggaa tgtccgctat ccccgcgcgc gctcgacctg
      301 gcggccaagg gcagacaacg ccgtctcgac gatgtgcgcg gcatcgaggc ccgcccactc
      361 gtactggcgc gccggggacg cctgctcgat gaagcggtcg ggcaggacca tcgggcggac
      421 cttcagcccg tggtcgagca tgccggtcga ggccaggtgc tgcagcacgt ggctggcgaa
      481 gccgccgatc gcgccctcct cgatcgtgat cagcacctcg tgctcgcgcg ccaggcggcg
      541 gatcaggtcg gtgtcgagcg gcttgcagaa ccgcgcgtcg gccacggtcg tgctcaggcc
      601 cttggcgccg agatcctcgg ccgccgtcag gcattcggcc aggcgcgtgc cgaagctcag
      661 gatggcgatc ttgccgccct cgcgcaggat ccggcccttg ccgatctcca gcggcgtgcc
      721 gcgcgccggc aggtcgacgc cgacgccctc gccgcgcgga tagcggaacg agcacggacg
      781 atcgtcgatg gcggccgccg tcgccaccat gtgcatcaat tcgagttcgt cggccggcgc
      841 catgacgacg aagcccggca ggcaggcgag atacgtcacg tcgaaggaac cggcgtgcgt
      901 ggcgccgtcg gcaccgacga ggcccgcgcg gtcgatggcg aagcgtaccg gcagcttctg
      961 gatggcgacg tcatgaacgc actggtcgta ggcgcgctgc aggaaggtcg agtagatggc
     1021 ggcgaacggc ttgaagccct cgcaggcgag gccggccgcg aaggtgacgc cgtgctgctc
     1081 ggcgatgccg acatcgaagg tgcgctcggg gaagcgctcg gcgaacttgt cgaggctggt
     1141 gcccgacggc atggccgcgg tgattgccac gaccttgtcg ctcgcctccg cctcggcgat
     1201 cagggccctg gcgaacaccg cctggtaggc cggcgcgttg gccaccggct tggcctgctt
     1261 gccggtgacg acgtcgaact tcaccacgcc gtggtacttg tcggcactcg cctcggccgg
     1321 cgggtagccc ttgcccttct tggtgacgac gtggatcagg atcggcttgt cgagcttgct
     1381 gtcgcgcgcg ttcttcagca ccggcagcag atggtcgagg ttgtgaccgt cgaccgggcc
     1441 gacatagtag aagcccatct cgtcgaacag cgtgccgccg gcgacgaagc cgcgcgcgaa
     1501 ctcctcggcg cggcgggccg ccatctcgag cggcttgggc agcgcctcgg cgaccgagcg
     1561 gccgaggtta cgaagcgtga cgtacggacg tcccgacagc aggcgcgaga ggtgcgccga
     1621 catcgccccg accggcgggg cgatcgacat gtcgttgtcg ttcaggatca cgatcagccg
     1681 gctgcgcagg tggccggcat tgttcatcgc ctcgtaggcc atgccggcac tcatcgcgcc
     1741 gtcgccgatc acggcgatca cgttgttgtt gctgccggcg agatcgcgcg ccaccgccat
     1801 gccgaggccg gccgagatcg aggtcgagga atgcgccgcg ccgaacgggt cgtactcgct
     1861 ctcggcgcgc ttggtgaagc cggacaggcc gccttcctgg cgcagcgtgc ggatgcggct
     1921 gcgccgcccc gtgacgatct tgtgcggata ggcctggtgg ccgacgtccc acagcagccg
     1981 gtcgcgcggc gtgtcgaaca cgtggtgcag cgccaccgtg agctcgacca cgccgagccc
     2041 cgcaccgaga tggccgccgg tcaccgacac cgcggaaatc gtctcctggc gcaactcgtc
     2101 ggcgagctgg cgcaactgct ccggcttcag ccgacggatg tcggccggca catcgacggt
     2161 atcaagaagc ggcgttttgc tctgcccggt catggaatca ctatagacca taagaagttg
     2221 agaagttgtt gagacgaacc ctcacgaacg acgggttacc acaaatctgg ccgcctgttt
     2281 caggaggtcg gccggtgtgc cgaaaggctc tagatgagcg gccgcctgtt gcgcaagtag
     2341 tgcagcctgc gaccgcgcgc gctccaggcc gaggatcgac acgaaggtgg ccttgccggc
     2401 gtcggcgtcc ttccgggtcg ccttgccgag ctcggccgga tcgccctcga cgtcgagcag
     2461 gtcgtcgacg atctggaacg ccaacccaag atcgtgcgcg taggccgcaa gtgcaaccct
     2521 cagcggctcg gccgccttgc ccaggatcgc tccggccgtg caggagaacg agatcagggc
     2581 gccagtcttc aggcgctgca ggcgggtgat cgcgccgatc gacatatcgg gctgggtttc
     2641 ggccagcagg tcgagcattt gtccgcccac catgccctgc gcgccggccg ccctggccag
     2701 ctccgaaacc agcgccgccc gcactgcggg atcgccgtgc gtcgcctcgt gcgccagcac
     2761 ctcgaaggcc agcgcctgca gggcatcgcc ggccaggatc gcggtcgcct cgtcgaattg
     2821 cttgtggcag ctgggttttc cgcgccgcag gtcgtcgtcg tccatcgccg gcaaatcgtc
     2881 gtggatcagc gaataggcgt gtacgaattc gatcgcgctg gcgacgcgca gcgccgacgt
     2941 ctcgctcacc ttgaacaggg tcgaactcgc cagcacgaag aaggcgcgca ggcgtttgcc
     3001 gccgcccagg ctcgaatagc gcatcgcctc gaacacgcgc gcctcgccag cctcgcctcg
     3061 cgggatcagg cgatccatgg tgcgctcggt cgactcggcg gc
//
LOCUS       DASRWS010000144         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3800535, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000144 DASRWS010000000
VERSION     DASRWS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3800535"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFF72_02590"
     CDS             complement(<1..621)
                     /locus_tag="VFF72_02590"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZV92074.1"
                     /translation="MDPGYHGPMADAQAFAIFPTCLGDCAVAWNDIGLIGIWLPDESA
                     ARLRVRVARRCAGIVEAAPPPAVSQAIAAMTRLVADGRADLRDIRIDDSMLDAFDRRV
                     CAAAREILPGRVLTYGQLAQRLGGAATARRVGESMARNRFPMVVPCHRVVASGGGLGG
                     FSAPGGHVTKRRLLVVERARLDGTADLFDDPPPDGVGASRRSGSESG"
     gene            738..1232
                     /locus_tag="VFF72_02595"
     CDS             738..1232
                     /locus_tag="VFF72_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZV92075.1"
                     /translation="MSRLPVHRPRRLSCGVVILNPNNEVLLCHVTGQRHWDLPKGGID
                     EWETPSQAALRETVEETGLVIAPERLIDLGRFGYTARKDLHLFATRMAHVEPREMCCV
                     STFVERGSNRTLPEMDGFGWFHVERVHGLCTSRMAALLSGELDLVGLAVRLREPSGAE
                     RLAA"
     gene            complement(1295..1909)
                     /locus_tag="VFF72_02600"
     CDS             complement(1295..1909)
                     /locus_tag="VFF72_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZV92076.1"
                     /translation="MVGISDYPSFVVAIIVFLLIPGPGNLALITSTGKGGRRGGLAAT
                     FGLIAGDQVLLWLAVAGVAALLAAYPAAFRGVQWLGAGYLAWLGSRMLVSPDGARPVM
                     TIRAGHYLRQAFLITLLNPKAIVFYMAFFPLFVDPGRHAGIATFAVMAATIAALTFLY
                     GACVTLLTHRLAERLRANPRIGRTMTRFAGTFMIVFGVKLALSR"
     gene            2095..3315
                     /locus_tag="VFF72_02605"
     CDS             2095..3315
                     /locus_tag="VFF72_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZV92077.1"
                     /translation="MAEAVASHALGTLVLSKNRHAGESLKSVRARLVGLRGRPTLSIV
                     YRHETREDTRNFEPDAGLAAIGALLDPQGETSFAHAVAQTPSGQWQLLTGRRGRQTLR
                     RIGVAGAVATKQPGEGGSGIALPAHDRPKVRALPLDLPFLAELGIADEGHRLVPSMAR
                     KWRQIDKFLEVLDDALDAAHFEGGAPLRVVDFGSGKGYLTFAVHEHLRRRFGVAPEVT
                     GVELRGELVELCNGAATRCAMEGLVFVEGDLRSVEPPAVDVMIALHACDTATDHAIDL
                     GMRAGARILVCSPCCHKELRPQMRRPAPLGALFRHGIHVAQEAEMVTDSLRALVLEAC
                     GFDAKVFEFVSLEHTRKNKMILATRRGEPSPDEGAALAEIEGLKSFYGIREQCLERLV
                     RERVGEPFRSRGRS"
     gene            complement(3293..>4169)
                     /locus_tag="VFF72_02610"
     CDS             complement(3293..>4169)
                     /locus_tag="VFF72_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZV92078.1"
                     /translation="ILPFDSATLSKAQYLLTGTMSRVGDKSRHALHIDLALTDLKNGT
                     VVAQASALARDQGLDHTPLAYYRDSPILVKDQVTDGYARTSSTPPGQHADAFYFDRIA
                     TATLISEATALYNQERYEPSLDLYRRALATPSGEQIRVLNGIYLATARLGRSAEAEQA
                     FGRIVAFGIAHNQLGVKFLFNPGSTEFWADPKVSGAYAMWLRQIAKESTAAHACMDIV
                     GHTSRTGSEQSNDVLSLQRANFIRQRLTADAPLLTERTKTSGMGFRQNIVGSGTDNAV
                     DALDRRVEFKIVPCS"
BASE COUNT          669 a         1364 c         1468 g          668 t
ORIGIN      
        1 tcccgattcg cttccggaac gccgcgatgc cccgacgcca tccggcggcg ggtcgtcgaa
       61 gagatcggcg gttccgtcaa ggcgagcccg ctcgacgacg agaagccgcc gcttcgtgac
      121 gtggccgccg ggcgccgaaa atccgccgag gccgcctccc gatgcgacga ctcggtgaca
      181 aggcaccacc atcggaaaac ggttgcgggc catcgattca ccgacccggc gcgccgtggc
      241 ggcgccgccg agccgctgcg ccagttgccc gtaggtgagc acgcggccgg gcaggatttc
      301 gcgcgcagcg gcgcagacgc gacggtcgaa tgcgtcgagc atggaatcgt cgattcgaat
      361 gtcgcgcagg tcggctcgcc cgtcggccac cagtcgcgtc atcgcggcaa tggcctggga
      421 gacggcaggc ggcggcgctg cctcgacgat gccggcgcag cgtcgcgcaa cccgcacgcg
      481 cagtcgggct gcgctttcat cgggcagcca gatgccgatc agtccgatgt cgttccatgc
      541 gacggcgcag tcgccgaggc aggtcggaaa gatcgcgaag gcttgcgcat ccgccatcgg
      601 tccatggtag ccggggtcca tgccgtccga atgcgtgcgt cggccatcgc gtcttcgtgt
      661 caaggaattg gcactgcgcg tcagggccat gccgtggagg cctgctcgcc ggcggtcgct
      721 cgcgggacaa tcgcaaggtg tcccgtttgc ctgtccatcg tccccgtcgt ctcagctgcg
      781 gcgtcgtcat cctgaatccg aacaacgagg tcctgctgtg tcatgtcacc ggtcagcgtc
      841 actgggacct gcccaagggc ggcatcgatg aatgggagac gccgagccag gcggcacttc
      901 gcgagaccgt ggaggaaacg ggcctcgtca tcgcgccgga gcggttgatc gatctcgggc
      961 ggttcggcta tacggcgcgc aaggacctgc atctcttcgc aacgaggatg gcgcacgtcg
     1021 agccgcgcga aatgtgctgc gtcagcacgt tcgtggagcg aggatcgaat cgcacgctgc
     1081 ccgagatgga cggcttcggc tggttccacg tcgagcgggt ccacgggctc tgcacatcgc
     1141 gaatggcggc ccttctgagt ggcgagctcg atctggtcgg gttggccgtc cgcctgcgcg
     1201 agccttcggg cgccgaacgt ctcgcggcct agaacgacgt ggtgcggcgc gggcatcgcg
     1261 tcggcgagcg gcagccacgt ctgcgcggcg gcgctcagcg gctgagcgcg agcttgacgc
     1321 cgaagacgat catgaaggtg ccggcgaatc gcgtcatcgt ccgtccgatg cgcggattgg
     1381 cgcgcaaccg ctcggcaagc cgatgtgtca gcagggtgac gcaggcgccg tagagaaagg
     1441 tcaacgcggc gatcgtcgcg gccatgacgg cgaacgtggc gattccggcg tgccgccccg
     1501 gatcgacgaa gagcgggaag aacgccatgt agaagacgat tgccttcggg ttgagaagcg
     1561 tgatcaagaa ggcctggcgc aggtagtgtc ctgcccggat cgtcatcacc ggccgggcgc
     1621 catcgggcga cacgagcatt cgcgagccga gccacgccag gtagccggca cccagccact
     1681 gcacgccccg aaacgccgcg gggtacgcgg cgagcagcgc ggcgacgccg gcgaccgcca
     1741 gccacagcag gacctgatca ccggcaatga gtccgaacgt cgcggccagg ccgccacgcc
     1801 tgccgccctt gcctgtcgat gtgatgagcg ccaggttgcc cgggccgggg atcaacagga
     1861 agacgatgat ggcgacgacg aacgacgggt aatcggagat gcccaccagc atgacgccat
     1921 tgtcggcgag tcgcgccgct tcctcgccga gaatcgccga gcggatccgc atccgttccc
     1981 gaatcgtttt cgaccttcgc accccgcgtc acgcccaaca ccgctgtggc cacgtcatcc
     2041 gtaccatccc agcccgcggc gccgacgacg ccacgcgagg agtttcttcg tgccgtggcc
     2101 gaggccgtcg caagccacgc gctcggcacc ctcgttctct cgaagaaccg ccatgccggc
     2161 gagagcttga agagcgtgcg cgctcgcctt gtcggtctgc gtggcaggcc gacgctgtcg
     2221 atcgtgtatc gccacgaaac gcgcgaggac acgcgaaact tcgagcccga tgcgggcctc
     2281 gcggcgatcg gcgccttgct cgatccgcag ggcgaaacgt cgttcgctca cgccgtcgcg
     2341 cagacgccga gcggccaatg gcaattgctc accggccggc gcgggcgcca gacgctgcgt
     2401 cgcatcggcg ttgccggtgc ggtcgccacg aagcagcctg gcgaaggcgg ctccggcatc
     2461 gcgctacctg cgcacgaccg gcccaaggtg cgggcgttgc cactggatct gcccttcctg
     2521 gccgagctcg gcatcgccga tgaaggacac cgactcgtgc cgtcgatggc ccgcaagtgg
     2581 aggcagatcg acaagttcct cgaagtcctc gacgacgcgc tcgacgcggc gcacttcgag
     2641 ggcggtgcgc cgttgcgcgt cgtcgacttc ggctccggca agggctacct gaccttcgcg
     2701 gtgcacgagc atttgcgccg ccgcttcggc gtcgcacccg aagtcaccgg cgtcgagctg
     2761 cgtggcgagc tggtggagct ctgcaacggg gcggcgacgc gctgtgcgat ggaaggcctc
     2821 gtcttcgtcg agggcgacct gcgcagcgtc gaaccgccag cggtcgacgt gatgatcgcg
     2881 ctgcacgcct gcgacaccgc caccgaccat gcgatcgacc tcggcatgcg tgccggcgcc
     2941 cggatactcg tctgctcgcc gtgctgccac aaggaactgc gaccgcaaat gcgaagaccg
     3001 gcgccgctgg gcgcattgtt ccggcatggc atccacgtcg cccaggaagc ggagatggtg
     3061 accgactcct tgcgcgcact cgttctcgag gcgtgcggct tcgacgccaa ggtcttcgag
     3121 ttcgtctcgc tcgaacacac ccgcaagaac aagatgatcc tggcgacccg ccgtggcgag
     3181 ccgtcgcccg acgaaggggc ggcgctcgcc gagatcgagg gactgaagtc tttctacggc
     3241 atccgcgagc agtgcctcga gcgcctcgtt cgcgagcgcg tcggcgaacc cttcaggagc
     3301 aggggacgat cttgaactcg acgcgccggt cgagcgcgtc gacggcgttg tcggtgccgc
     3361 tgccgacgat gttctgtcga aagcccatgc ccgaggtctt ggtgcgctcc gtcaggagcg
     3421 gcgcatcggc ggtcaggcgc tgccggatga agttggcgcg ttgcagcgag aggacgtcgt
     3481 tggattgctc ggaaccggtc cggctggtgt gcccgacgat gtccatgcag gcgtgcgcgg
     3541 cggtgctttc cttggcgatc tggcgcagcc acatggcata ggcgccgctg accttggggt
     3601 cggcccagaa ctcggtgctg cccgggttga agaggaactt gacgccgagt tggttgtggg
     3661 cgatgccgaa ggcgacgatc cggccgaacg cctgctcggc ctcggcgctt cgacccagcc
     3721 tggcggtcgc aaggtagatg ccattgagga cgcggatctg ctcgcccgac ggcgtggcca
     3781 gggcgcggcg atagaggtcc agcgatggct cgtagcgttc ctggttgtag agcgcggttg
     3841 cttcgctgat cagcgtcgcc gtcgcgatgc ggtcgaaata gaaggcgtcg gcgtgctggc
     3901 ccgggggtgt cgaagacgtg cgcgcgtagc cgtcggtgac ctggtccttc acgaggatcg
     3961 gactgtcgcg gtaatacgcc agcggggtgt gatcgagtcc ctggtcgcgc gccagcgccg
     4021 aggcctgggc gacgacggtg ccgttcttca ggtcggtgag cgccaggtcg atgtgcaggg
     4081 catgtcggct tttgtcgccg acgcgactca tcgttccggt gagaaggtat tgggccttcg
     4141 acaaggtggc cgagtcgaat ggcaggatc
//
LOCUS       DASRWS010000145         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_635113, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000145 DASRWS010000000
VERSION     DASRWS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635113"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFF72_02615"
     CDS             <1..234
                     /locus_tag="VFF72_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92079.1"
                     /translation="YRVADELKAANVRKVPAWKAWLTHAAAAASAAGCTCLLVLFLLP
                     TGAAEIRPTANATSPSPGCAEPTPPPVRPRDRR"
     gene            767..1090
                     /locus_tag="VFF72_02620"
     CDS             767..1090
                     /locus_tag="VFF72_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZV92080.1"
                     /translation="MSKLSKFEELALADKDVKFGFDNYDVLRQIGSLVREMRVSGGFS
                     QTALQEMSGIPQADISRVESGTMERGPSLLTLVRLAHAAGKRLVIGIEDEEGEDKKTT
                     RILAL"
     gene            1101..1400
                     /locus_tag="VFF72_02625"
     CDS             1101..1400
                     /locus_tag="VFF72_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92081.1"
                     /translation="MTLENWSDLSGLLGSLALVVTAWRNDGLYGFIDKLRAAVKKAGD
                     DAKAASREATEPLADPVLKSLESELTKWSWVDRWSLRAGAFLLVLSYFLRLAFQH"
     gene            1746..2090
                     /locus_tag="VFF72_02630"
     CDS             1746..2090
                     /locus_tag="VFF72_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92082.1"
                     /translation="MPRPLQFGDTAPYAVKGGALRLGERLTLARKRRRLTLRELAAKA
                     GISYDTARATEAGNLQTGLGAYLAMLWAMGLESEIDGLADPDHDQTGKQLDLSRQPRR
                     VTHSKEKHDGDF"
     gene            2077..3378
                     /locus_tag="VFF72_02635"
     CDS             2077..3378
                     /locus_tag="VFF72_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HZV92083.1"
                     /translation="MATSERRDRDRRAYVYLQMPQSLEIVTAGYYELDFPQGVATGSF
                     VYSPAYLDRKDALPIEPYELPLSPRRATTIHLKGIFGALRDASPDAWGRRIIEKHVGH
                     ADLTEVDYLLHSPEDRAGALSFGRGKTPPGPLREFNQVVRLEALLAAAETFLAQDQVG
                     AQDIPEQLFELLRPGTSMGGARPKNVVEDGEGLWLAKFPDPGDRWSNARVEGAMLALA
                     AECGIRTAAYRVQCVAGKDVLLVKRFDRTRTDEDYLRHRMVSGLTILGAEDTYGDRSK
                     WSYLLLADELRRRSARPAEDLIELFRRMTFNALISNTDDHPRNHALIAPTEKWELSPA
                     YDLTPNPLVSQEKRDLAMACGRFNRYANRTNLLSEHAQFKLSLEQARALVDKVQTVVA
                     ERWLPVLRQHGAPEADCDRIAPAFNYPGFELDPVTVLAARA"
     gene            complement(3566..4585)
                     /locus_tag="VFF72_02640"
     CDS             complement(3566..4585)
                     /locus_tag="VFF72_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HZV92084.1"
                     /translation="MSPHDIDGVLVQWGERLFYPRNRIVRAASTPRLGGSVGQRAAAI
                     RQRIQATVVRRAPQVMVKVTGGGRGMAAIAAHFRYISKSGRLAFEDDRGVVREGKEAL
                     RDLAEQWRHGGSFIDETSHRREAFNIMLSMPSGTDPLVVQHAAREFARAELGDHRYVM
                     VLHDHQANPHVHISVRAESREGWRLNPRKADLQRWRETFAEKLRGWGIDAEATRQRTR
                     GQWRHYEPLWRVKAAQEGRLETPLRTRKSGDGIDRDRAVAAEAWSRIAEGLSRSDNAE
                     DKALAEQVDRFLGSTFAVAPKRRLDKEPEPQLRTKTVRQPLREPLRESPRIIARNRDG
                     PDRGR"
BASE COUNT          805 a         1574 c         1516 g          801 t
ORIGIN      
        1 taccgggtcg cagatgagct gaaagcggcg aatgtgcgca aggtacctgc gtggaaggcc
       61 tggctgacgc atgcggcggc cgcggccagc gctgccggct gcacgtgcct gctcgtgctg
      121 ttcctcctgc ccacgggcgc tgccgaaatc cggccgacgg ctaacgcgac ctccccctct
      181 ccgggctgcg cggagccgac accacctcct gtgaggccgc gcgatcggcg ctgaggccgg
      241 ggagcgcctg cgccttgtcg ggcatcgggg cgcgcacacg catgaacggc atcgcacgga
      301 cgtattgctc gatgtcttgc gggctgagcg agctcttatg tcggccagga acgaatccct
      361 gtcggtgccc ctcttgactt ctatgtggta aagcacataa tatcgtatgc agaagttcaa
      421 cgctgcggcc cagcaagagg cgatggacat cctcaacagg cagcgcgtgg agtgaagcag
      481 agcctgggca tgatgattca acgccatctg cggatttcaa gcggcgtgga gccgctgagt
      541 gcgtcggagt tctgcagcgc cggggcaggt gtttcatcgc tgtacgaggt cgactattcg
      601 cacagtctct ggtctcgcgt gatgtgcgtc tacgagacgg ttggcgcaga cttcacgctc
      661 ctggccatag acgcatatca agggaccgcg cgactgggac ttccgacatc tgccccgaat
      721 gcgagggccc gtgcgtgggg ccgctcatct tgaaagggaa acgatcatga gcaagctttc
      781 caaattcgaa gagctggccc ttgccgacaa ggacgtgaag ttcggcttcg acaactacga
      841 cgtcctgagg cagatcggca gcctcgttcg ggaaatgcga gtcagtggag ggttctctca
      901 gactgcgctt caggagatga gcggaattcc gcaggccgat atcagtcggg tcgagtccgg
      961 cacgatggag cgtgggccat cgctgctcac cctggttcgc cttgcccatg cggccggcaa
     1021 gcgcctcgtc atcgggatcg aggatgagga aggcgaggac aagaagacga cgcgcatcct
     1081 ggccctctga acgccttgcc gtgacgctcg agaactggtc cgatctgagc ggccttctcg
     1141 gaagcctcgc gctcgtcgtc actgcatggc gaaacgacgg gctgtacggc ttcatcgaca
     1201 agcttcgtgc cgcagtcaag aaagcaggcg acgacgcgaa ggctgcctct cgcgaagcga
     1261 cggaaccgct cgcggatccc gtcctcaagt ccctcgagtc cgagttgacg aagtggtcgt
     1321 gggtcgaccg ctggagcctt cgcgccggcg cattcctgct ggtgctctca tactttctgc
     1381 gattggcttt tcagcactga caccagtgag gtgccgacac ccgctccgag cagagaggcg
     1441 ttgccaagct tcttttccaa gcctctcgca gctgtctgaa gccaagatgg acgaaggctg
     1501 aggcaatgcg ttcgcaatcc cgctccgaaa cacctgctgc gccggccgtg tcgtgccaca
     1561 tgcggctgac ttgagcttcc atagcgtcga tcaaatagcg ccgctggcgt gccgccgtga
     1621 aggacggact gctgaaacaa acttatttgc ggataagatt gtcttcggtc gatcttttcc
     1681 tgacgacgtc tccttcgcat aggtatactg aaacattccc agaatcgttt tcgactggtt
     1741 tcagcatgcc gcgtcctctc cagttcggtg ataccgcgcc ctacgcggtc aagggcggtg
     1801 ccctgcgctt gggcgaacgc ctcaccctcg cccgcaagcg ccggcgcctg acgctgcgcg
     1861 agctggccgc caaggccggc atcagctacg acaccgcccg ggctaccgaa gcgggcaacc
     1921 tccagaccgg cctcggtgcc tacctcgcca tgctgtgggc gatgggcctc gaatcggaga
     1981 tcgacgggct ggccgatccg gatcacgacc agaccggcaa gcagctcgac ctgtcgcggc
     2041 agccacgccg agtgacgcac agcaaggaaa agcacgatgg cgacttctga acgtcgcgat
     2101 cgcgatcgcc gggcctacgt ctatctgcag atgccgcagt cgctcgagat cgtcaccgcg
     2161 ggctactacg agctcgactt tccgcaaggt gtggccacgg gctcgttcgt ctacagcccg
     2221 gcctacctcg atcgcaagga cgcgctgccg atcgaaccgt acgagttgcc gctcagccct
     2281 cgccgggcca cgaccatcca cctgaagggc atcttcggcg cgctgcgcga cgcctcgcca
     2341 gacgcgtggg gtcgacgcat catcgagaag cacgtgggcc atgccgatct caccgaggtc
     2401 gactacctgc tgcactcccc cgaggatcgc gccggcgcgc tgtcgttcgg gcgcggcaag
     2461 acgccacccg ggccgctgcg cgagttcaac caggtggtcc ggctcgaagc gctgctcgcc
     2521 gccgccgaaa ccttcctcgc gcaggaccag gtcggcgcac aggacatccc ggagcaattg
     2581 ttcgaactgc tgcgccccgg cacgtcgatg ggcggcgcca ggccgaagaa cgttgtcgaa
     2641 gacggcgagg gcctctggct cgccaagttc ccggaccccg gcgaccggtg gagcaacgcg
     2701 cgcgtggaag gcgccatgct ggcgctggcc gccgaatgcg gaatccgcac cgcggcgtat
     2761 cgcgtccaat gcgtggccgg caaggatgtc ttgcttgtga agcgattcga ccgcacccgc
     2821 accgacgagg actacctgcg ccatcgcatg gtgagcggcc tgacgattct cggtgccgag
     2881 gacacctacg gcgatcggag caagtggtcg tacctgctgc tggccgacga gctgcgccgg
     2941 cgcagcgcgc gcccggcgga agacctgata gagctcttcc gacgcatgac gttcaacgcg
     3001 ctcatctcga acaccgacga ccatccgcgc aaccatgcgc tgatcgcgcc gaccgagaag
     3061 tgggagctct cgccggcgta cgacctcacc cccaatccac tcgtgagcca ggaaaagcgc
     3121 gacctcgcga tggcctgcgg gcgcttcaac cgctacgcca accgcacgaa cctgttgtcc
     3181 gagcacgcac agttcaagct ttcgctcgag caggcgcgcg cgctcgtcga caaggtgcag
     3241 accgtcgtcg ccgagagatg gctgcccgtg ctgcggcagc acggtgcacc cgaggccgac
     3301 tgcgaccgca tcgcaccggc cttcaactac ccgggcttcg agctcgaccc cgtgaccgta
     3361 ctcgccgcgc gagcctgaat ccttgcggcc cggcttcggg cgtggcgggt ggccgaggtg
     3421 tcgatctggc ccggcgtgag cagcgcacag cgctgcgagg aacgacggcc agttcggcag
     3481 cggtctcgtt cacgcgcagc gtgaaggaaa gcaagaccgc gaacctcgac cacgggaacg
     3541 ggccgcactg gtcgcttctc cggcgctatc gcccgcgatc cggaccgtcg cgattccttg
     3601 cgatgattcg cggcgactcc cgtaacggct cgcgcagcgg ctgccgcacg gttttcgtcc
     3661 tgagctgcgg ttcaggttcc ttgtcgagcc tgcgcttggg agcaaccgcg aaggtgctgc
     3721 cgagaaaccg atcgacctgc tcggccagtg ccttgtcttc ggcattgtcc gaacgcgaga
     3781 ggccttcggc gatccggctc caggcttccg cggcgacggc tcggtccctg tcgatgccgt
     3841 cgcccgactt cctcgttcgc agaggcgtct cgaggcggcc ctcctgcgcc gccttgacgc
     3901 gccagagcgg ctcgtagtgg cgccactggc cgcgcgtgcg ctgccgcgtc gcttccgcgt
     3961 cgatgcccca gccgcgaagc ttctccgcga aggtttcgcg ccagcgctgc aggtcggcct
     4021 tgcgcgggtt gagccgccag ccctccctcg attcggcacg cacgctgatg tggacgtgcg
     4081 ggttggcctg gtggtcgtgc agcaccatca cgtagcggtg gtcgccgagc tcggcccggg
     4141 caaactctcg cgcagcgtgc tgaacgacga gcggatcggt gccactcggc atcgacagca
     4201 tgatgttgaa tgcctcgcgc cggtgactcg tctcgtcgat gaacgagccg ccatgccgcc
     4261 actgctcggc caggtcgcgc agcgcttcct tgccttcgcg gacaacgccg cggtcgtcct
     4321 cgaacgcgag ccggccgctc ttgctgatgt agcgaaagtg cgcggcgatc gccgccatgc
     4381 cacgaccgcc gccggtgacc ttgaccatca cctgaggtgc gcgacggacc accgtcgcct
     4441 ggatgcgctg ccggatcgcc gcggcccgct ggccgacgct gccgccaagg cgaggcgtcg
     4501 aagccgccct gacgatgcgg ttgcgtggat agaagagccg ctcgccccac tggacaagga
     4561 cgccatcgat gtcgtggggg ctcatcgaac gactggcgga ccgccgaagg gcttgccctg
     4621 ctgccgcacc ggagatgaac gcgtgggtcg aagctctgac aacaggcgag acgccagccc
     4681 cacgtgttgg cgcacg
//
LOCUS       DASRWS010000146         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2353410, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000146 DASRWS010000000
VERSION     DASRWS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353410"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFF72_02645"
     CDS             complement(<1..436)
                     /locus_tag="VFF72_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HZV92085.1"
                     /translation="MFDVLVYLYENYWRPDACPEHDQLARKLTSVGFESEEIEDALTW
                     LDGVAAAAEARVGEQRADSLRVYAKSELEHLGEASIGFISFLESAGVLPPPMREMVID
                     RASAIPGGPLDLEDLKIIVLMVFWSLGEEPDALILDELFVDEA"
     gene            complement(519..1634)
                     /gene="dprA"
                     /locus_tag="VFF72_02650"
     CDS             complement(519..1634)
                     /gene="dprA"
                     /locus_tag="VFF72_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZV92086.1"
                     /translation="MIDRDELAAWLRLLEAPGIGRQAVRLLLREFGSPQAVVSAPAAS
                     LRRRVGDASASALSSESADSSRLLAKTLAWIDAPVDETRDVLALGDARYPQSLLQTAD
                     PPLLVYVRGSVEPLNAASLAIVGSRNPTPQGLENARAFATHLSRSGLVVVSGLALGID
                     AAAHEGALDAAGTTIAVVGTGLDTVYPTRHRALADRIVQSGGAIVSEFAIGTPALPQN
                     FPIRNRIIAGLARGTLVVEAAVQSGSLITARLALESGRDVFAIPGSIHSPQARGCNAL
                     IKQGAKLVDAAADILEEYGVLVQPVASGRDIEAQADAGREDPLVEALGFDPIGLDALV
                     ARTGMGAAELSARLLELELAGRVARLPGQLFQRIERA"
     gene            complement(1684..>2281)
                     /locus_tag="VFF72_02655"
     CDS             complement(1684..>2281)
                     /locus_tag="VFF72_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZV92087.1"
                     /translation="GDIAYVRGNLGNEREYRIFREPKPLVDPTTKKILGYEATYVGSS
                     EYTAQGETRTGADGKVEIIPATFLVTSIRQEAGVGDRLSPVPAREYTNYAPHPPAGPM
                     SGQIVSIYGDGLTAGQNQIVSLNRGADEGLERGHVLALFHDGKVIVDPTDKSRTRIKL
                     PDEREGLLFVFRVFEHMSYALILSVKQPVVVGDRFTQP"
BASE COUNT          351 a          770 c          823 g          337 t
ORIGIN      
        1 ccgcctcgtc gacgaaaagc tcgtcgagga tgagcgcgtc gggctcttcg ccgagcgacc
       61 agaacaccat caggacgatg atcttcaggt cctcgagatc gagcgggccg ccggggatgg
      121 cgctcgcgcg gtcgatcacc atctcgcgca tcggcggcgg cagcacgccg gccgactcga
      181 gaaaggagat gaatccgatc gacgcttcgc ccaggtgttc gagctcgctc ttcgcgtaga
      241 cgcgaaggct gtcggcacgc tgttcgccga cgcgcgcctc ggccgccgcg gcgacgccat
      301 cgagccaggt cagcgcgtct tcgatttcct cggactcgaa gccgaccgac gtcagctttc
      361 gggccagctg gtcgtgctcc gggcaagcgt cgggacgcca gtagttctcg tagaggtaca
      421 cgagcacatc gaacatcgaa acactatacc cgagcgcctc ttcccgacgc cgggccgcgg
      481 acgaacggca ccggattccg cgcccctggc gagggcggtc aggcccgctc gatgcgctgg
      541 aagagctgcc ccggcagccg cgcgacgcgg cccgccagct cgagctcgag caatcgcgcc
      601 gacagctcgg cggcgcccat gccggtgcgc gccaccagcg cgtcgaggcc gatcgggtcg
      661 aacccgagcg cttcgacgag cgggtcttcg cggccggcgt ccgcctgtgc ctcgatgtcg
      721 cgtcctgagg cgacgggctg cacgagcacg ccgtactcct cgaggatgtc ggcggcagcg
      781 tcgacgagct tggcgccctg cttgatgagc gcattgcaac cgcgcgcctg cggcgaatgg
      841 atcgagccgg gaatcgcgaa gacgtcgcgg cccgattcga gcgccagccg cgccgtgatc
      901 agcgagcccg attgcactgc ggcctcgacg accagcgttc ctcgtgccag gccggcgatg
      961 atgcgatttc ggatcgggaa gttctggggc agcgccggcg tgccgatcgc gaattcgctg
     1021 acgatggcgc cgcccgactg aacgattcga tcggcgagcg cgcgatgcct cgtcggatag
     1081 accgtgtcga ggccggtgcc aacgacggcg atcgtggtcc ctgccgcgtc gagcgcgccc
     1141 tcgtgcgcgg ctgcgtcgat gccgagcgcc aggcccgaca cgacgacgag acccgatcgg
     1201 ctcaggtgcg tcgcaaaggc gcgcgcattc tcgaggccct gcggcgtcgg attgcggctg
     1261 ccgacgatgg cgagcgacgc ggcattgagc ggctcgaccg acccgcgcac gtagacgagg
     1321 agcggcgggt cggcggtctg cagcaatgac tgggggtagc gcgcatcgcc gagggccagc
     1381 acgtcgcgtg tctcgtcgac cggcgcgtcg atccaggcga gggtcttggc cagaaggcgg
     1441 gacgaatcgg ccgattcgct cgacagggcg gaagcggagg cgtcgccgac ccggcgccgc
     1501 aggctggccg ctggcgccga aacgactgcc tggggcgatc cgaactcgcg cagcaggagc
     1561 cgcacggcct gccggccgat gccgggcgcc tcgagaaggc gaagccaggc ggcgagctcg
     1621 tcgcggtcga tcatcgacgc gtctgcggcc gcaccggacc ggggccgacg cggcgcgccg
     1681 ggctcagggc tgggtgaagc ggtcgccgac gacgaccggc tgtttcaccg acaggatgag
     1741 cgcgtacgac atgtgctcga agacgcgaaa gacgaagagc agaccctcgc gctcgtcggg
     1801 cagcttgatc ctggtgcgcg acttgtcggt cgggtcgacg atgaccttgc cgtcgtgaaa
     1861 gagcgccagc acgtggccgc gctcgaggcc ttcgtcggcg ccgcggttga gcgagacgat
     1921 ctggttctgg cccgccgtca gcccgtcgcc gtagatggac acgatctgac ccgacatcgg
     1981 ccccgcgggc ggatgcggcg cgtagttcgt gtattcgcgc gccggcaccg gcgacagccg
     2041 gtcgcccacg cctgcctcct ggcggatgct cgtgacgagg aaggtggccg ggatgatctc
     2101 gaccttgccg tcggcgccgg tgcgcgtctc gccctgcgcg gtgtattccg acgagccgac
     2161 gtaggtcgcc tcgtagccga ggatcttctt ggtcgtcgga tcgacgagcg gcttgggctc
     2221 gcggaatatc cggtattcgc gctcgttgcc caggttgccg cgcacatagg cgatgtcgcc
     2281 a
//
LOCUS       DASRWS010000147         6086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_635824, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000147 DASRWS010000000
VERSION     DASRWS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635824"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFF72_02660"
     CDS             complement(<1..573)
                     /locus_tag="VFF72_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV92088.1"
                     /translation="MTMLEEPRPAGTVGEKTYQRVRADIVCGVLPPGERLTLDRMRDV
                     YEASVGTLRENLNRLSSEGLVVAEGSRGFQVTPISVVNLREVAAMRCLLETHALRESF
                     EAGDMEWEGSVVAAHHKLAAMEQRMKAGARARAQDWKRYDREFHRALIAACGSQVLLE
                     THAVIYDRYLRYQLVAGVYRGDVAAGEHRRL"
     gene            746..1732
                     /gene="dctP"
                     /locus_tag="VFF72_02665"
     CDS             746..1732
                     /gene="dctP"
                     /locus_tag="VFF72_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZV92089.1"
                     /translation="MSSTIARRTLLRAGASGLALSAAPSLFAQGTMKMRLSTAFTEQD
                     MRADAYKAFGAAMKSDFQLEPYWGNTLFKQGTELVALQRGNLEMANLAPQDIAKQLPA
                     WSLLTSAYLFRDVAHLQKTFKSDVGREFVKMARDKLDIEVITPVYFGARQVNLKPDRV
                     IRTPADLHGIKLRMPPGEFWQFLGESIGVNPTPVAFAEVYTALQTGAIDGQDNPLILS
                     KLMKFDEVTTQFVLTSHVIGFDVMTVSSKAWNALKPDVQARFRSEADKAIGDYTVKFE
                     GKESEVVSALKAEGKKVYKPDVNAFRTYAQKRYLDKYGSDWPKGALEKINAL"
     gene            1747..2277
                     /locus_tag="VFF72_02670"
     CDS             1747..2277
                     /locus_tag="VFF72_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZV92090.1"
                     /translation="MSTDASASGTATGRWLQRRANDIAVLLIATMFVSFLLQIVFRYA
                     LDKPLGWTEEVTVLCWVWIVLWGASFIVAERDEVRFDIVYNLVPLKLQRVFTIASSLG
                     MIALMGLSLPATWRYVAFMRREHSAYLGMRFDYLYSIYLLFAVVCIVRQARVTWQAWR
                     GVPLHGAADELTERAE"
     gene            2274..3575
                     /locus_tag="VFF72_02675"
     CDS             2274..3575
                     /locus_tag="VFF72_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZV92091.1"
                     /translation="MTGLSPFTMCVIILVVLALAGLPIGLSMIGASIFYLLASGLDPG
                     TVAEQILNGLYNSYVLLAVPLFILAADLMNTGSLTDRLLKFCLVLVGRFRGGLGHVNI
                     VSNIIFAGMSGSAIADAVGIGRIIIGMMTRGGKYPVAYAAAITASAAIIGPIIPPSIP
                     MVIYALVSDASIGYLFLGGVVPGLLLGVAFMVMNAVISRRRAYPVEAPVALREIPRIT
                     FDAIPALLLPVILLVGIYGGVMTPTEAAAAAAAYALFAAAVIYRAMTGRQLYGVLLSS
                     AKATTTVGMLIAGALAFNYVVTAENIPVSVQHFVAGFHLSKVGFLLLVNAILLILGCL
                     LEGTTILLIIVPIFIPTVKALGIDTVHFGVVVVVNIMIGLLTPPFGLLLFITANMTRQ
                     PLVAIVREATPFIVAAIAMLALITFVPETVLWLPRVMGYKG"
     gene            3603..4067
                     /locus_tag="VFF72_02680"
     CDS             3603..4067
                     /locus_tag="VFF72_02680"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZV92092.1"
                     /translation="MKLPIYVLNGPNLNRLGQREPDIYGHTTLAQIEALCRRTAGDRA
                     LEFRQTNSEAQLIDWIHEAIDRACGIVINPAAWSFTSIAILDALKMFPGPIIELHISN
                     IHRREEIYHRSLMSRAVTAVIAGLGAQGYAVAVQAVVERTDGSGAATATRPA"
     gene            4210..5661
                     /locus_tag="VFF72_02685"
     CDS             4210..5661
                     /locus_tag="VFF72_02685"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZV92093.1"
                     /translation="MTSDTQAQAGLAALTARLHEDLGWLEWPAAPWLPKRHDAAGHGA
                     IDVAIIGGGMAGLAAAASLRRLGIAAPIFDRAAAGFEGPWATTARMETLRSPKQLTGP
                     ALGLPALTFRAWFEAQYGLDAWAALDRIPRLLWMDYLRWYRRALDLEVNNESTVRAIV
                     PRADGFVELGIEAAGSVRTVLARRVVLATGRDGLGGAYVPELAAALPRDRWAHSSDLI
                     DESSLAVSRIGVVGAGASAMDSAATALEAGAASVDLLIRRADIPRVNKGKGAGNPGLT
                     FGWVGLPDTWKWRIRHYLNEQQTPPPRNSTLRVSRHANARFHLGCAIRDLRWNGGTIE
                     VDSSTGRFEFDFMIFSTGFRIDWHARPEFAAIAPHVRSWGDRFTPPPGEDDRELADSP
                     DLAAAFELQEKVPGSCPGLGRIHCFCFPAALSHGTVSGDIPAISDGATRLAQGIAGLF
                     YREDIDYHFARLQAYAEPEVFGDEWTPAPPPAR"
     gene            5683..>6086
                     /locus_tag="VFF72_02690"
     CDS             5683..>6086
                     /locus_tag="VFF72_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZV92094.1"
                     /translation="MNSASTSSPPGDDVVDRVAVLVDGGPLQSLRRERDKVRQATQGS
                     YGALFDPTLQGLTVADRLLVALSASRLTPVPALAAHYRERLERQQVDAGGVDPAALHA
                     ADGSGPVEAVAARLQALLVFARTLVERPIEGDR"
BASE COUNT          916 a         2192 c         1949 g         1029 t
ORIGIN      
        1 caggcggcgg tgctcgccgg cggcgacgtc gccacggtac acgcccgcca cgagctggta
       61 gcgcagatac cggtcgtaga tgacggcgtg cgtctcgagc agcacctgcg agccgcaggc
      121 cgcgatcagc gcgcggtgga attcgcggtc gtaacgcttc cagtcctgcg ccctggcgcg
      181 cgcgcccgcc ttcatccgtt gctccatcgc cgccagcttg tgatgcgcgg cgacgacgct
      241 gccctcccac tccatgtcgc cggcctcgaa cgattcacgc aacgcatggg tctcgagcag
      301 gcagcgcatc gcggccacct cgcgcagatt gaccaccgag atcggcgtca cctggaaccc
      361 gcgcgatcct tcggcgacga ccagtccttc cgacgacagc cggttcaggt tctcgcgcaa
      421 ggtgccgacg ctcgcctcgt agacatcgcg catccggtcg agcgtcagcc gttctccggg
      481 cgggagcacg ccgcagacga tgtcggcgcg tacgcgctga taggtcttct caccgacagt
      541 tccggccggc cgtggctctt ccagcatggt catggtcttc gccgtttcgt ccccgggagg
      601 gccgaggcag gtcgatcata ggtcaatcgg cgattcatct atcgtcgaag cctattgttt
      661 tcatccgatg aagaagctag cctccatccg tcgcccgccg catcggccgg ccgccccaca
      721 cccccaccct ccggagatcc gacccatgtc atcgaccatc gcacgccgca ccctcctgcg
      781 cgccggcgca tccggcctgg ccctttccgc cgctccctcg ctctttgcgc agggcacgat
      841 gaagatgcgc ctgagcaccg cgttcacgga gcaggacatg cgtgccgatg cctacaaggc
      901 cttcggcgcg gcgatgaaga gcgacttcca gctggagccg tattggggca acaccctgtt
      961 caagcagggc accgagctgg tggcgctgca gcgcggcaac ctcgagatgg ccaacctcgc
     1021 tccgcaggac atcgccaagc agttgcccgc atggtcgctc ctgacctcgg cctacctgtt
     1081 ccgcgatgtg gcccacctgc agaagacgtt caagagcgac gtcggccgcg agttcgtcaa
     1141 gatggcccgc gacaagctcg acatcgaggt gatcaccccg gtctacttcg gcgcccgcca
     1201 ggtcaatctc aagcccgacc gcgtcatccg cacgccggcc gacctgcacg gcatcaagct
     1261 gcgcatgccg ccgggcgagt tctggcaatt cctgggcgaa tcgatcggcg tcaatccgac
     1321 gccggttgct ttcgccgagg tctacaccgc cttgcagacc ggagcgatcg acggccagga
     1381 caacccgctg atcctcagca agctcatgaa gttcgacgag gtgacgacgc agttcgtcct
     1441 caccagccac gtcatcggct tcgacgtgat gaccgtctcg tcgaaggcat ggaacgcgct
     1501 caagcccgat gtgcaggcgc ggtttcgcag cgaggcggac aaggcgatcg gcgactacac
     1561 cgtcaagttc gagggcaagg agagcgaagt cgtcagtgcc ttgaaggcgg aaggcaagaa
     1621 ggtgtacaag cccgacgtca acgccttccg cacctacgcg cagaagcgct atctcgacaa
     1681 gtacggcagc gactggccca aaggcgcgct cgagaagatc aacgccctgt agacgggcgc
     1741 ttcgacatgt cgaccgacgc ctcggcctcg ggcaccgcga ccgggcgctg gctgcagcga
     1801 cgtgccaacg acatcgcggt cctgctgatc gcgacgatgt tcgtctcgtt cctgctgcag
     1861 atcgtctttc gctacgcgct cgacaagccg ctcggctgga ccgaggaggt gacggtcctg
     1921 tgctgggtgt ggatcgtgct ctggggtgcc tcgttcatcg tcgccgaacg cgacgaggtg
     1981 cgcttcgaca tcgtctacaa cctcgtgccg ctcaagctcc agcgcgtctt cacgatcgcc
     2041 tcgagcctcg gcatgatcgc gctgatgggg ttgtcgctgc cggcgacctg gcgctacgtc
     2101 gcgttcatgc gccgcgagca ctcggcctat ctcggcatgc gcttcgacta cctctactcc
     2161 atctacctgc tgttcgcggt cgtctgcatc gtccgccagg cccgggtgac gtggcaggcg
     2221 tggcgcggcg tgccgctgca cggcgccgct gacgaactga cggagcgcgc cgaatgacgg
     2281 gcctgtcgcc cttcacgatg tgcgtgatca tcctggtcgt gctcgcgctg gccgggctgc
     2341 cgatcgggct gtcgatgatc ggcgcctcga tcttctacct tctcgcctcg ggcctcgatc
     2401 ccggcaccgt cgccgagcag atcctcaacg gcctgtacaa cagctacgtg ctgctcgcgg
     2461 tgccgctctt catcctggcc gccgacctga tgaacaccgg cagcctgacc gaccggctgc
     2521 tgaagttctg cctggtgctg gtcggccggt tccgcggcgg gctcggccac gtcaacatcg
     2581 tctccaacat catcttcgcc ggcatgtccg gctcggcgat cgccgacgcc gtcggcatcg
     2641 gccggatcat catcggcatg atgactcgcg gcgggaagta tccggtcgcg tacgcggcgg
     2701 cgatcactgc atcggcggcg atcatcggtc cgatcatccc gccgtcgatc ccgatggtga
     2761 tctacgcgct cgtgtccgac gcgtcgatcg gctatctctt cctcggcggc gtcgtccccg
     2821 gcctgctgct cggcgtggcc ttcatggtga tgaacgccgt gatctcgcgt cgccgcgcct
     2881 acccggtgga agcgccggtg gcgctgcgcg agataccgcg catcaccttc gacgccattc
     2941 ccgccctgct gctgccggtc atcctgctgg tggggatcta cggcggcgtc atgacgccga
     3001 ccgaggccgc cgccgccgcc gcggcctatg cactcttcgc cgccgccgtc atctaccgcg
     3061 cgatgaccgg tcgccagctc tacggcgtgc tgctctcgag cgcgaaggcg acgacgaccg
     3121 tgggcatgct gatcgccggg gccctcgcct tcaattacgt cgtcaccgcc gagaacatcc
     3181 cggtctccgt gcagcacttc gtcgccggat tccacctgtc gaaggtcggc ttcctgcttc
     3241 tcgtcaacgc catcctgctc atcctcggct gtctcctcga gggaacgacc atcctgctga
     3301 tcatcgtgcc gatcttcatc ccgaccgtga aggcgctcgg catcgacacg gtccacttcg
     3361 gcgtggtcgt ggtcgtcaac atcatgatcg gcctgctgac cccgccgttc ggactgctgc
     3421 ttttcatcac cgccaacatg acccgccagc cactggtcgc gatcgttcgc gaggcgacgc
     3481 ccttcatcgt cgcggcgatc gcgatgctgg cgctgatcac cttcgttccc gaaaccgtgc
     3541 tctggctgcc gcgcgtcatg ggctacaagg gctgagcggc ggcgccaagg acaccccacc
     3601 ccatgaagct gccgatctac gtgctcaacg gccccaatct caatcgcctc ggccagcgcg
     3661 agcccgacat ctacggccac acgacgctgg cgcagatcga ggcgctgtgc cggcgcaccg
     3721 caggcgaccg ggcgctcgag ttccggcaga cgaacagcga ggcgcagctg atcgactgga
     3781 tccacgaggc gatcgaccgc gcctgcggca tcgtcatcaa ccccgccgcc tggtcgttca
     3841 cctcgatcgc gatcctcgac gcgctgaaga tgttccccgg accgatcatc gagctgcaca
     3901 tctccaacat ccatcggcgc gaggagatct atcaccgctc gctgatgtcg cgcgcggtga
     3961 ccgcggtcat cgccggtctc ggcgcgcagg gctacgcggt ggcggtccag gccgtcgtcg
     4021 agcgcaccga cggctccggc gccgcgacgg caacgcgccc cgcctgaaga tcgagggcga
     4081 ctcccggccg cctgcccgca tccgcatccg catccgcaac cgcatccgtc gtccgcatcc
     4141 gtcatcgtcg gcgacgggcc ggcgagcggc gaatcgccct gcgtcgatgt gttgtagtct
     4201 cgatgcagga tgacgagcga cacgcaggcg caggcaggac tggcggcatt gacggcgcgg
     4261 ctgcacgagg acctcggctg gctcgagtgg cccgcggcgc cctggctgcc gaagaggcac
     4321 gacgccgccg gccacggcgc gatcgatgtg gccatcatcg gcggcggcat ggcggggctc
     4381 gcggcagcgg catcactcag gcgcctgggc atcgcggcgc cgatcttcga tcgcgccgcc
     4441 gccggcttcg aagggccctg ggcgaccacg gcgcgcatgg agacgcttcg ctcgcccaag
     4501 cagctcacgg gaccggcgct cgggctgccg gccctcacct ttcgcgcctg gttcgaagcg
     4561 cagtacgggc tcgacgcgtg ggcggcgctc gaccggattc cgcgcctgct ctggatggac
     4621 tacctccgct ggtaccgccg ggccctcgat ctcgaggtga acaacgagag cacggtgcgc
     4681 gccatcgttc cgcgcgccga cggtttcgtc gagctgggca tcgaggccgc cggcagcgtc
     4741 cggaccgtgc tcgcccgccg cgtcgtcctg gcgaccggcc gcgacggact cgggggtgcc
     4801 tatgtccccg aactcgcggc cgcgttgccg cgcgatcgct gggcccattc gtccgacctc
     4861 atcgacgaga gctcgctggc cgtctcgcgg atcggggtcg tcggcgcggg cgcctcggcg
     4921 atggacagcg ccgccaccgc gctcgaggcc ggcgcggcgt cggtcgactt gctcatccgc
     4981 cgcgccgaca tcccgcgcgt caacaagggc aagggcgccg gcaaccccgg gctcaccttc
     5041 ggctgggtcg gcctgcccga cacgtggaag tggcgcatcc ggcactacct caacgagcag
     5101 cagacgccac cgccgcgcaa cagcacgttg cgcgtctcgc gccacgccaa tgcgcgcttc
     5161 cacctcggct gtgcgattcg cgaccttcgc tggaacggcg ggacgatcga agtcgactcg
     5221 tcgacgggcc ggttcgaatt cgacttcatg atcttctcga ccggctttcg catcgactgg
     5281 catgcgcggc cggagttcgc ggccatcgcg ccgcatgtcc ggtcctgggg cgatcgcttc
     5341 acgccgccgc ccggcgagga tgaccgcgag ctcgccgatt cgcccgatct cgcagccgcc
     5401 ttcgagctgc aggaaaaggt gccggggagt tgtcccggcc tcgggcggat ccactgcttc
     5461 tgctttccgg cagcactcag ccacggcacc gtgtcgggcg acattccggc gatcagcgac
     5521 ggcgcgacgc gcctggcaca aggcatcgcc ggcctcttct atcgcgaaga catcgactac
     5581 cacttcgcgc gcctgcaagc ctatgccgag cccgaggtgt tcggcgacga atggacaccg
     5641 gcgccgccgc cggcgcgatg atgtgagcat gaggagatgc cgatgaattc cgcctcgacg
     5701 agttcgcctc cgggcgacga cgttgtcgat cgagtcgccg ttctcgtcga cggcggcccg
     5761 ctgcagtcgt tgcgccgcga gcgcgacaag gtgcgccagg cgacgcaggg cagctacggc
     5821 gccctgttcg atccgacgct gcaagggctg acggtcgccg accgcctgct ggtggcgctg
     5881 tcggcgagcc ggctgacgcc ggtgccggcg ctggcggctc actatcgcga gcgactcgag
     5941 agacagcagg tcgacgccgg cggcgtcgat ccggcagcgc tccatgcggc cgacggcagc
     6001 gggccggtcg aagctgtcgc tgcgcgtctg caggccttgc tcgttttcgc ccgcaccctg
     6061 gtcgagcgcc cgatcgaggg tgaccg
//
LOCUS       DASRWS010000148         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2073944, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000148 DASRWS010000000
VERSION     DASRWS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073944"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFF72_02695"
     CDS             complement(<1..1139)
                     /locus_tag="VFF72_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZV92095.1"
                     /translation="MAKIPVLIAGGGPVGLALAVELGMAGISCLLVERRDGSVTVPKM
                     SGLSVRSMELSRRWGIADKAKRAGWPQTRPNDFVYCTSMIGQELTRIRIPAYVDKHPS
                     FTPEPDCGCAQIFFDPILLEKARSLPSVTLRHLTNLDSFAQDEHGVRATITDRKTNKS
                     EVIEAQYLAGCDGADGAVATALDPDYEGVGVFAKSTNIYFRSRELMEMHDKGWARFFR
                     FTDAGGSWGELIGIDGEETWRLSSLNAVTGYEPRDYMRRLAGRDFNYEIISVMDWERR
                     ERVATRYRAGKRVFIAGDAAHQNSPTCGLGLHTGLQDAVDLGWKLTAMLQGWGGEQLL
                     DSYGIERKPVALNNVRACTGQFELLAALPTGREIDDETPSGSALRA"
     gene            complement(1159..2214)
                     /locus_tag="VFF72_02700"
     CDS             complement(1159..2214)
                     /locus_tag="VFF72_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZV92096.1"
                     /translation="MTTIFLLASLSAHAADWKADWDATVAAANQEGALVLSVPSGRAW
                     RDALAKFSDAYPQIEIEMTPVASRDFWPRIIKEHEAGQYLWDLRIGGIDAPSYDMKNR
                     GELAPVRPLFVLPEITDEKVWQAGFDGLFLDKEKKYFPAFVAYESQSVYYNKTKIADT
                     SKLTMQTLDDPQWSGKMSMADPRGGASLNTLTVLDKLYGDEPIKKLMAQKPVITGEPR
                     QMMDWIASGRYPIAFGLPSATLVEYQQRGASIADFGRVPGLRIWSPGVGAIEYLAKAP
                     HPNAAKVFVNWLLAKDTQAMLMQAVKLNSQRLDVPQGDPAEAVNAKEMSQYTCTQAEE
                     LEPYEQRVVALLRQTLP"
BASE COUNT          415 a          768 c          801 g          446 t
ORIGIN      
        1 gcgcgcaacg cgctgcccga cggcgtttcg tcgtcgatct cccgaccggt cggcagcgcc
       61 gccagcaact cgaactggcc cgtgcaagcg cggacattgt tgagcgcgac gggcttgcgc
      121 tcgatcccat agctgtcgag aagttgctcg ccgccccagc cttgcagcat cgctgtgagt
      181 ttccagccga ggtccacggc gtcttgcaag ccggtgtgaa gcccaagccc gcaggtcggc
      241 gagttctgat gcgcggcgtc gccggcaatg aagacgcgct tgcccgcgcg atatcgcgtc
      301 gcgacccgct cgcgccgttc ccaatccatc accgagatga tctcgtagtt gaaatcgcgc
      361 ccggcgaggc ggcgcatata atcacgcggc tcgtatccgg tcacggcgtt gagcgacgag
      421 agccgccagg tctcttcgcc gtcaatgccg atgagctcgc cccaggagcc gccggcgtcg
      481 gtgaagcgga agaaccgcgc ccaccccttg tcgtgcatct ccatcaactc gcgcgagcgg
      541 aaatagatgt tggtgctctt cgcaaagacg ccgacgccct catagtcggg atcgagcgcg
      601 gtggcgacag ctccgtcggc gccgtcgcag ccggcgagat attgggcctc gatgacctcg
      661 ctcttgttgg tcttgcgatc ggtaatggtc gcgcgtacgc cgtgttcgtc ctgtgcgaag
      721 gagtccaggt tggtgagatg gcgcagcgtc accgagggca gcgagcgcgc cttttcgagc
      781 aggatcgggt cgaaaaagat ctgtgcgcag ccgcaatcgg gctcgggcgt gaaggaagga
      841 tgcttatcga catacgccgg gatgcggata cgggtcaact cctgcccgat catgctagtg
      901 caataaacga aatcgttggg gcgggtctgc ggccagccgg cgcgcttcgc tttgtcggcg
      961 attccccagc ggcggctgag ctccatgctg cggacggaaa gcccgctcat cttcggcacg
     1021 gtgaccgagc cgtcgcggcg ctcgaccagc aagcaggaaa tgcccgccat gccgagctcg
     1081 acggcgaggg cgaggccgac cgggccgccg cccgcgatca gtaccgggat tttcgccatc
     1141 ggtgccaggg ctcgcggctc agggcagggt ctggcgcagc agcgcgacga cgcgctgctc
     1201 gtaaggctcg agctcctcgg cctgggtgca ggtgtactgg ctcatctctt tggcgttgac
     1261 cgcttcggcg ggatcgcctt gcggcacgtc gaggcgctgg ctgttgagct tcaccgcctg
     1321 catcagcatc gcctgcgtgt ccttcgccaa gagccaattg acgaagacct tggcggcgtt
     1381 gggatgcggc gccttggcga gatactcgat cgcgccgacg cccgggctcc agatgcgcaa
     1441 gcccggcacg cggccgaaat cggcgatcga ggcgccgcgc tgctgatatt ccaccaaagt
     1501 cgcgctcggc aggccgaaag cgatcggata gcggcccgag gcgatccaat ccatcatctg
     1561 ccgcggctcg ccggtaatca ccggcttttg cgccatcagc ttcttgatcg gctcgtcgcc
     1621 atagagcttg tccaacacgg tgagcgtatt cagcgaggcg ccgccgcgcg gatcggccat
     1681 cgacatcttg ccggaccatt gcggatcgtc gagtgtctgc atcgtgagct tcgacgtgtc
     1741 ggcgatcttg gtcttgttgt agtagacgct ctggctctca taggcgacga aggcggggaa
     1801 gtatttcttc tccttgtcga ggaagaggcc gtcgaaaccg gcctgccaga ctttctcgtc
     1861 ggtgatctcg ggcaggacga agagcggccg cacgggcgcc agctcgccgc gattcttcat
     1921 gtcgtaagac ggcgcgtcga tgccgccgat acgcagatcc cagagatact gtcccgcctc
     1981 gtgctccttg atgatgcgcg gccagaaatc gcggctcgcg accggcgtca tctcgatctc
     2041 gatctgcgga taggcgtccg agaatttcgc cagggcgtcg cgccaggcgc ggcccgaggg
     2101 caccgagagg acgagcgcgc cttcctggtt ggcggcggcg acggtcgcgt cccaatcggc
     2161 cttccaatcg gcggcatggg cggaaagaga cgccagtaaa aaaatcgtcg tcattgcgag
     2221 gccgagcttg acgaggccga cgcaatccag cctggatcgc cacgaccgct tcgcggtctc
     2281 gcggtgacag taaaaaatat ggcgcaagat tctattccgc cgcggccgga ggcttgtgct
     2341 gccggctctt ccagatgtcg tgatagtcga cattgtgcgg cacgatctcg gagacgacga
     2401 cgatatgcga catgtcgttc tgcgccggcg
//
LOCUS       DASRWS010000149         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3231365, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000149 DASRWS010000000
VERSION     DASRWS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231365"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFF72_02705"
     CDS             <1..860
                     /locus_tag="VFF72_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZV92097.1"
                     /translation="PVPGYDASLYRSERLMATAGDGAQVPVSLVWREDTRRAGPRPLL
                     LYGYGSYGVPLDPWFSQSRVSLLDRGVVFAIAHVRGGGDLGRSWYEAAKMASKATSFG
                     DFVACAEMLIERGITAASMLAIEGGSAGGLLMCVAVNLRPELFRAAVVEVPFVDVVNT
                     MLDETLPLTTGEFLEWGNPKLPEQYAWLRAYSPYDNLRRGDYPSMFVRSGINDTQVAY
                     WEPAKYVAKLRTLKTDRRPLLLKVDLEVGHGGASGRFDALREIAENDTFLLVELGLAQ
                     VSDQRPRLA"
     gene            complement(908..2191)
                     /locus_tag="VFF72_02710"
     CDS             complement(908..2191)
                     /locus_tag="VFF72_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HZV92098.1"
                     /translation="MSGVLRKLVKAAGRAQRRGLKDATRAFRQAMAASVSIAAAGAPA
                     PRPKAKPRRASSRPAVPAKRERSSAWTPTHRAASAPGAWPPGFRVALEPAARTAPARP
                     DDAAGRFSEGRFTELDGTRSFKLFEPDGFEAGGRLPLVVMLHGCTQSADDFAAGTRMN
                     RLAQERGFHVLYPQQAPRSNANKCWNWFVPADQRRGSGEPALLAALTRQVIATHRIDA
                     DRVYVAGLSAGGAMAAILAREYPDIYAAAGVHSGLQAGAAHDVGSAFEAMRSGTTLPS
                     TGGSRPRKIDATVAASASAPGDSLPHADADASTGAGCGAPLIVFHGDADSTVHAANGA
                     HVVDSALGDARWIAERSVDLPATGRRGSTRIVYRAASSGIPSRTEHWVVHGAAHAWSG
                     GDASGSFTDPRGPDASREMLRFFFEHPRVRSPATS"
BASE COUNT          319 a          771 c          799 g          325 t
ORIGIN      
        1 agccggtacc cggctatgac gcgtcgctct atcgaagcga gcggctgatg gcgacggccg
       61 gcgacggggc gcaggtgccg gtctccctgg tctggcgcga ggacaccagg cgtgccggcc
      121 cgcggccgct gcttctctac ggctacggca gctacggtgt tccgctcgat ccgtggttct
      181 cgcagtcccg cgtgtcgctg ctcgaccgcg gcgtcgtttt cgccatcgcc cacgtccgcg
      241 gtggcggcga cctcggccga agctggtacg aggccgcgaa gatggcgagc aaggccacca
      301 gcttcggcga cttcgtcgcc tgcgccgaga tgctgatcga gcgcggcatc acggcggcct
      361 cgatgctggc catcgaaggc ggcagcgccg gtggcttgct gatgtgcgtc gcggtgaacc
      421 ttcggcccga gctctttcgc gccgccgtcg tcgaggtgcc gttcgtcgac gtcgtcaaca
      481 ccatgctgga cgagacgctg ccgctgacca cgggagagtt cctcgaatgg ggcaacccca
      541 agctgcccga gcagtatgcg tggctgcgcg cgtacagccc ctacgacaac cttcgacgcg
      601 gcgactaccc gtccatgttc gtgcgcagcg gcatcaacga cacgcaggtc gcgtactggg
      661 agccggccaa gtacgtggcc aagttgcgca cgctcaagac cgaccggcgg ccactcctcc
      721 tcaaggtcga cctcgaggtc ggccacggcg gcgcgtcggg tcgtttcgac gcgttgcgcg
      781 aaatcgccga gaacgacacc ttcctgctgg tcgagctcgg tctcgcgcag gtgagcgatc
      841 agagaccgcg cctcgcgtag cgacggtgcc gaagggcccg gcgcctgcgg gcggccatga
      901 gcccggttca gctcgtcgcg ggtgagcgga cgcgcggatg ctcgaagaag aaccgaagca
      961 tctcgcgcga cgcatcgggg ccgcgcggat cggtgaagct gcccgacgcg tcgccgcccg
     1021 accacgcatg cgcggcgcca tggaccaccc agtgctcggt tcggctggga atcccggacg
     1081 aggcggcgcg atagacgatg cgggtgctgc ccctgcgtcc ggtcgcaggc agatcgacgc
     1141 tgcgctcggc gatccagcgc gcatcgccga gggccgagtc gaccacatgc gcgccgttgg
     1201 cggcgtgcac ggtgctgtcg gcgtcgccat ggaagacgat cagcggagcg ccacacccgg
     1261 cgccggtcga ggcatcggca tcggcatgcg gcagcgagtc gcccggcgcg gatgccgacg
     1321 cagcgaccgt cgcgtcgatc ttccgcggcc tcgaccctcc tgtcgacgga agtgtcgttc
     1381 ccgaccgcat cgcctcgaaa gccgagccga cgtcgtgggc ggccccggct tgcagacccg
     1441 agtggactcc cgccgcggcg tagatatcgg gatattcgcg ggccaggatc gccgccatcg
     1501 cgcctcctgc cgacaagccg gcgacgtaga cgcgatcggc gtcgatgcga tgcgtcgcga
     1561 tgacctggcg tgtcagcgcg gcgagcaacg ccggctcgcc ggagccgcgc cgctggtcgg
     1621 cgggaacgaa ccagttccag cacttgttgg cgttcgatcg cggcgcctgt tgcgggtaga
     1681 ggacgtggaa gccgcgctcc tgcgcgagcc ggttcatccg cgtgccggcg gcgaagtcat
     1741 cggcgctctg ggtgcagcca tggagcatga cgacgagcgg cagccggccg ccagcctcga
     1801 aaccgtcagg ctcgaacagc ttgaacgagc gggtcccgtc gagctcggtg aagcggcctt
     1861 cgctgaagcg tccggcagcg tcgtcgggcc gggccggcgc agtcctcgct gcaggctcga
     1921 gggcgacacg aaatccgggc ggccacgcgc cgggagcgct tgcggcgcgg tgcgtcggcg
     1981 tccaggccga cgacctctcc cgcttcgccg ggactgcggg ccgcgacgag gcacgccgcg
     2041 gtttggcttt cggccggggc gcgggcgcgc cggccgcggc gatcgagacg gatgccgcca
     2101 tcgcctggcg aaatgcccgc gtcgcgtcct tcaggccgcg ccgctgggca cggcccgccg
     2161 ccttgacgag ctttcgaagg acgccgctca agcggagctc ttcacttggg acgc
//
LOCUS       DASRWS010000150         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2074176, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000150 DASRWS010000000
VERSION     DASRWS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074176"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1210
                     /locus_tag="VFF72_02715"
     CDS             176..1210
                     /locus_tag="VFF72_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZV92099.1"
                     /translation="MKRRSLIQGAAAAMLGAPGLAAIAQQTVTLKFHTFMAPQSNVWL
                     TMHKPWMAKVEKESNGRIKFEAYPAMQLGGTPVQLYDQARDGVVDVIWTVCGSTAGRF
                     PRVEVFELPFIMTNAEATSKAYWDYVQTNAPDEFKETQTLALHVHGPGLFHSAKKLIK
                     SPADLRGMKVRGPTRQITKLLGALGATPVGMPLPGIPDALSKGTIDACVIPWEVVPSI
                     KVDELARQHTEFPSNQRALYTTTFVMAMNKPKYEGLSAENKKVIDANSGRETSAWLGK
                     TQQGNDGNGRKVSLDHGGTIYTLTDAEAEPFHKVGKPIDDEWVADMDKRGHDGRKLLE
                     SARSLITKYT"
     gene            complement(1247..1996)
                     /locus_tag="VFF72_02720"
     CDS             complement(1247..1996)
                     /locus_tag="VFF72_02720"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HZV92100.1"
                     /translation="MSYILDALRRADEERERGAVPSLHSQHYAGASDDDERGTRRPLV
                     LVGAALAATVIVALVVALAWVSRGGAAAPRVAAQGPVTTPPPAMPAAAATTATAAMPI
                     ASGQVAPATPVAPTAPQPATAPQPAARGGVKRPDARERRELARALASMKAERRGGTRS
                     TTSTPATAPRMPAPNVGTGGRLPDELRRELPTLAFGGASYSKDPASRMVIFNGRIFHE
                     GDTIAPQLVLDRIEPKSAVFSYKGYRYEVEF"
     gene            complement(2019..>2794)
                     /locus_tag="VFF72_02725"
     CDS             complement(2019..>2794)
                     /locus_tag="VFF72_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZV92101.1"
                     /translation="SASASASAAGASHASSVPSTLAAVDPQAAPARPASAAADAGSAG
                     EDSAGPIRTVAALKADLKSLGHDEKSAWRELASLWKVDARGGDACAAATRQQIRCFKF
                     NATLPMLKSLDRPGFLGLRDEGRNVDVVLVGLEGDDALLQSEGSGTRRVSLAAIARLW
                     HGEFGTLWRLPPGYTHALGEGDSGPLVDRLAEQLAHLAHEPPPGNGQTMDAALRARVV
                     RFQTSHGLSGVGKAGPTTFMQLNRAVGVDEPTLRTVATD"
BASE COUNT          432 a          956 c          982 g          424 t
ORIGIN      
        1 ctaccgaggg gtcatgccgt tcctcgccag cgacgccctg cgcaccttgc tgctgcttct
       61 tttcccgccg atctcgctct gggtcctgcg ctttgtcggg tagggaaaat cgccagcgaa
      121 gacaccgact gtttgcccga aactcgacgt gtcaccggtc caaggagcat tgccaatgaa
      181 acgtcgctcg ctcatccagg gtgccgccgc cgccatgctc ggtgcgcccg gcctcgccgc
      241 gatcgcccag cagacggtga cgctgaagtt ccataccttc atggcgccgc agtcgaacgt
      301 ctggctgacg atgcacaagc cgtggatggc caaggtcgag aaggaatcga acggccgcat
      361 caagttcgag gcctacccgg cgatgcagct cggcggcacc cccgtgcagc tctacgacca
      421 ggcgcgcgac ggcgtcgtcg acgtcatctg gacggtctgc ggcagcaccg ccggccgctt
      481 tccgcgcgtc gaggtcttcg agctgccgtt catcatgacc aacgccgagg cgacgtcgaa
      541 ggcttactgg gactacgtcc agaccaacgc ccccgacgag ttcaaggaga cgcagacgct
      601 ggcgctgcac gtgcacgggc cgggcctctt ccattcggcg aagaagttga tcaagtcgcc
      661 cgccgacctg cggggaatga aggtgcgcgg accgacgcgg cagatcacca agctgctcgg
      721 cgcgctcggc gccacgccgg tcggcatgcc gctgccgggc atccccgatg cgctctcgaa
      781 gggcacgatc gacgcctgcg tcattccgtg ggaagtggtg ccgtcgatca aggtcgacga
      841 gctggccagg cagcacaccg agttcccgtc gaaccagcgg gcgctctaca cgacgacgtt
      901 cgtgatggcg atgaacaagc ccaagtacga gggactgtcg gccgagaaca agaaggtgat
      961 cgacgccaac tcgggtcgcg agacgtctgc ctggctcggc aagacccagc agggcaacga
     1021 cggcaacggg cgcaaggtct cgctcgatca cggcggcacg atctacaccc tgaccgacgc
     1081 cgaggccgag cctttccaca aggtgggcaa gccgatcgac gacgagtggg tcgccgacat
     1141 ggacaagcgc gggcacgatg gcaggaagct gctcgaaagc gcccgctcgc tgatcaccaa
     1201 gtacacctga gagccgggca acggccgcct tgcccggcgg ccggattcag aactcgacct
     1261 cgtagcgata gcccttgtac gagaagacgg ctgacttcgg ctcgatgcgg tcgaggacga
     1321 gttgcggtgc gatcgtgtcg ccctcgtgga agatgcggcc gttgaagatg accatccggc
     1381 tggccggatc cttgctgtag ctcgcgccgc cgaaggcgag cgtcggcaat tcgcggcgca
     1441 actcgtcggg cagcctgccg ccggtgccga cgttcggcgc gggcatccgt ggtgcggtcg
     1501 caggcgtcga tgtcgtgctt cgcgtgccac cccggcgttc ggccttcatc gaggccagag
     1561 ccctcgcaag ctcgcgcctt tcgcgggcat ctggccgctt gactccgccg cgtgccgccg
     1621 gctgtggcgc tgtcgccggc tgcggcgctg tcggcgcgac cggcgtcgcc ggcgcgacct
     1681 ggccggaagc gatcggcatg gccgctgtcg cggtcgtcgc cgcggccgca ggcatggccg
     1741 gcggcggggt cgtgaccggt ccctgggcgg cgacccgcgg tgccgcggcg ccaccgcgcg
     1801 agacccatgc gagcgcgacg acgagggcca cgatcactgt cgccgcgagg gctgcaccga
     1861 cgaggacgag cggtcttcgc gtcccgcgtt cgtcgtcgtc ggaagcgcct gcgtagtgct
     1921 gcgagtgcag gctcgggacc gcgccgcgtt cgcgctcctc gtcggctcgg cgcaaggcat
     1981 cgaggatgta cgacatggtt cgttgtcctc ggacccggtc agtcggttgc gacggtgcgc
     2041 agcgtcggct cgtcgacgcc gacggcgcga ttgagctgca tgaaggtggt cgggccggcc
     2101 ttgccgacgc cgctcagccc gtgactcgtc tggaagcgga ccaccctggc gcgcagcgcc
     2161 gcgtccatcg tctggccgtt gccgggcggc ggttcatggg cgaggtgggc gagctgctcg
     2221 gccagccgat cgacgagggg acccgaatct ccctcgccga gggcgtgcgt gtagccgggc
     2281 ggcaagcgcc agagcgtgcc gaactcgccg tgccagagcc tggcgatcgc ggccagggaa
     2341 acgcgacgcg tgccgctgcc ctcgctttgc agcagcgcgt cgtcgccttc gagaccgacg
     2401 aggacgacat cgacgttgcg tccttcgtcg cgcaggccga ggaagccggg ccggtcgagg
     2461 ctcttcagca tcggcagggt cgcgttgaac ttgaagcagc gaatctgctg gcgggtcgct
     2521 gccgcgcagg cgtcgccgcc gcgcgcgtcg accttccaga gcgacgccag ctcgcgccat
     2581 gccgacttct cgtcgtggcc cagtgacttg aggtcggcct tgagcgccgc gacggtacgg
     2641 atgggaccgg cggaatcttc gccggccgat cctgcgtcgg cggccgccga cgcgggccgg
     2701 gccggcgccg cctgcggatc gaccgccgcg agtgtcgacg ggacggacga ggcatgcgat
     2761 gcacccgccg ccgacgccga cgccgacgcc gacg
//
LOCUS       DASRWS010000151         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3801894, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000151 DASRWS010000000
VERSION     DASRWS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801894"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFF72_02730"
     CDS             <1..181
                     /locus_tag="VFF72_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZV92102.1"
                     /translation="LTSTVSMMSWAMVKMLVSVVMLALAPRIVAGLSWPAMLATLVVC
                     MQTYGLALLWRRRPA"
     gene            225..1124
                     /gene="atpB"
                     /locus_tag="VFF72_02735"
     CDS             225..1124
                     /gene="atpB"
                     /locus_tag="VFF72_02735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZV92103.1"
                     /translation="MAAEEHAALTPGEYVIHHLTHLSNARPKGLVDFSVVNFDSVFFS
                     VLLGAITCFVFWLAARKATSGVPGRFQAAVEFLFELVDNEAKGMIHNARSRRFVAPLA
                     LTVFVWVTLLNTMDLLPVDLLPSIWGWLYGVTGHNPEHAYLRVVPTADLSVAMGLSVG
                     VLLLSLFYSIKIKGLGGWLHELYVAPFGTIKLTANPLTWVAFLLLSIANVAMQFIEYV
                     SKTFSHGMRLFGNMYAGELLFLLIAMLGGAFALSVGGFALAALHIVAGVAWAIFHILI
                     IVLQAYIFMMLTLVYIGQAHDSH"
     gene            1191..1460
                     /gene="atpE"
                     /locus_tag="VFF72_02740"
     CDS             1191..1460
                     /gene="atpE"
                     /locus_tag="VFF72_02740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZV92104.1"
                     /translation="MELIGLVAVAAGLMIGLGALGACVGVGIMASKYLESAARQPELM
                     GVLQTKVFLLLGLIDASFIIGAGIALWYTTANPFLAQIQSLPIAK"
     gene            1531..2001
                     /locus_tag="VFF72_02745"
     CDS             1531..2001
                     /locus_tag="VFF72_02745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZV92105.1"
                     /translation="MSITSTFIIQIIVFLVLAWFTMKFIWPPITSALDERAQKIREGL
                     SAADKAKAELTAANKRVEEELSSARTDAAQRLADAERLAHSMIEEAKGRANEEGAKIV
                     AAARAEAEQEASKARETLREQVAALAVEGAEQILRREVNPQVHAELLDRLKAEL"
     gene            2006..2551
                     /locus_tag="VFF72_02750"
     CDS             2006..2551
                     /locus_tag="VFF72_02750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZV92106.1"
                     /translation="MAEIATIARPYAEALLKAVAPADAAALAGEVGAIGRVADDAQLR
                     QFADNPKIGPAQVLELILSVAEMPSGGAPSDAAKNLLGTVIDNGRLAALPEIAAQFQS
                     LVDAKSGTSQATIESAFPIDDAQLAAVTTAMERRFGRKLDAHVVVDPALIGGVRVVVG
                     DEVLDTSIRARLAQMKAALVA"
     gene            2636..>3177
                     /locus_tag="VFF72_02755"
     CDS             2636..>3177
                     /locus_tag="VFF72_02755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZV92107.1"
                     /translation="MQLNPAEISELIKSRIQGLAVSADIRNEGTVVSVTDGICRIHGQ
                     SDAMQGEMLEFPDNTFGLALNLERDSVGSVILGEYEHITEGDTVKCTGRILEVPVGPE
                     LIGRVVDSLGRPIDGKGPLNAKETDVIEKVAPGVIWRKKVAQPVQTGLKSIDAMVPIG
                     RGQRELIIGDRQTGKTAVAI"
BASE COUNT          517 a         1074 c         1033 g          553 t
ORIGIN      
        1 cctgaccagc acggtgagca tgatgagttg ggcgatggtg aagatgctgg tgtcggtcgt
       61 catgctggcc ctggcaccga gaatcgtcgc cgggctcagc tggccggcga tgcttgcgac
      121 cctggtggtc tgcatgcaga cctacggact tgcgctgctc tggcgtcgca ggcccgcctg
      181 atcgaacgag actctggacc gcaacgacat tcgcgattcg acacatggca gccgaagaac
      241 acgcagcgct cacccccggt gagtacgtca ttcaccacct cacgcacctg agcaatgcca
      301 ggcccaaggg gctggtcgat ttctcggtcg tcaatttcga ttcggtcttc ttctcggtcc
      361 tgctcggcgc gatcacctgc ttcgtgttct ggctggcggc gcgcaaggcc acctcgggcg
      421 tgcccgggcg cttccaggcg gcggtggaat tcctcttcga gctggtcgac aacgaagcca
      481 aggggatgat ccacaacgcg cgcagccgca ggttcgtcgc gccgctcgcc ctcacggtgt
      541 tcgtctgggt gaccctgctc aacacgatgg acctgctgcc ggtcgatctg ctgccctcga
      601 tctggggctg gctctacggc gtcacgggcc acaaccccga gcacgcctac ctgcgcgtgg
      661 tgccgacggc cgatctctcg gtggcgatgg gtctttccgt gggcgtgctg ctgctgagcc
      721 tcttctacag catcaagatc aaggggctgg gcggctggct gcacgagctc tacgtcgcgc
      781 ccttcgggac gatcaagctg acggccaacc cgctcacgtg ggtcgccttc ctgctgctgt
      841 cgatcgccaa cgtggcgatg cagttcatcg agtacgtgtc caagaccttc tcgcatggga
      901 tgcggctgtt cgggaacatg tacgccggag agctcctctt cctgctgatc gccatgctgg
      961 gcggcgcatt cgcgctctcg gtgggcggct tcgcgctggc cgcgctgcac atcgtcgccg
     1021 gtgtcgcctg ggcgatcttc cacatcctga tcatcgtcct gcaggcctac atcttcatga
     1081 tgctgacgct ggtctacatc ggccaggccc acgattcgca ctgacgccgc gcgccggcaa
     1141 gctcttttcc cttcgtcctt tcccctttct caccaaactc tggagtcaac atggaactca
     1201 tcggtctcgt cgcggtcgct gccggcctca tgatcggtct cggtgctctc ggcgcttgcg
     1261 tcggcgtcgg catcatggcc agcaaatatc tcgaatcggc ggcgcgccag cccgagttga
     1321 tgggcgtgct gcagacgaag gtgttcctgc tgctcggcct gatcgacgcc tcgttcatca
     1381 tcggcgccgg catcgcgctg tggtacacga cggccaatcc gttcctggcg cagatccaga
     1441 gcttgccgat cgcgaagtaa tccgggcgct ggcgcaggcc gtcgcggcgt tcgccgcggc
     1501 gcgcgccgtc accctcgcca aggtcacacc gtgagcatta cctccacctt catcatccag
     1561 atcatcgtgt tcctggtcct ggcctggttc acgatgaagt tcatctggcc gccgatcacg
     1621 agcgcgctcg acgagcgggc gcagaagatc cgcgaaggcc tgtctgcggc cgacaaggcc
     1681 aaggcggagc tgaccgcggc caacaagcgc gtcgaggaag agctgtcttc tgcccgcacc
     1741 gacgctgcgc aacgcctggc cgacgccgag cgcctggccc actcgatgat cgaggaggcc
     1801 aagggccgcg ccaatgaaga gggcgcgaag atcgtcgccg cggcgcgcgc cgaggccgag
     1861 caggaagcgt cgaaggcgcg cgagaccttg cgcgagcagg tcgccgcgct cgccgtcgag
     1921 ggagccgagc agatcctgcg ccgcgaggtc aatccccagg tgcatgccga gttgctcgac
     1981 cggctgaagg cggagctctg aggccatggc cgagatcgcc accattgcgc gaccgtacgc
     2041 cgaggcgttg ctgaaggccg tggcgcccgc cgacgcagcc gcgctcgccg gcgaggtcgg
     2101 cgcgatcggg cgggttgccg acgacgcgca gctgcgccag ttcgccgaca acccgaagat
     2161 cggtcctgcg caggtgctcg agctgatcct ctcggtcgcc gagatgccgt ccggcggcgc
     2221 cccgagcgac gcggcgaaga acttgctcgg caccgtcatc gacaacggcc ggctcgcggc
     2281 gttgcccgag atcgccgcgc agttccagtc gctcgtcgac gccaagagcg gcacctcgca
     2341 ggcgaccatc gagagcgcct ttccgatcga cgatgcgcag cttgccgcag tcacgacggc
     2401 gatggagcgg cgcttcggcc gaaagctcga cgcgcacgtc gtcgtcgacc cggccctgat
     2461 cggcggcgtg cgcgtggtcg taggcgacga agtcctcgac acctcgatcc gcgcccgcct
     2521 ggcgcagatg aaggcggcgc tcgtcgcctg agcggcgagt ccgtttcaac tgcgccgttt
     2581 cgcgaacact tgagtttcaa ccagcctgcc cgaactgcag tgcgaaggtc ctgacatgca
     2641 actgaatccc gctgaaatct ccgagctgat caagagccgc atccaggggc tcgccgtgtc
     2701 ggccgacatc cgcaacgaag gcaccgtcgt ctcggtgacc gacggcatct gccgcatcca
     2761 cggccagtcg gacgcgatgc agggcgagat gctcgagttc cccgacaaca ccttcggact
     2821 ggcgctgaac ctcgagcgcg actcggtcgg ctcggtgatt ctcggcgagt acgagcacat
     2881 caccgaaggc gacaccgtca agtgcacggg tcgcatcctc gaagtgccgg tcggacccga
     2941 gctgatcggc cgcgtcgtcg actcgctcgg ccgtccgatc gacggcaagg gcccgctgaa
     3001 cgcgaaggag acggacgtca tcgagaaggt cgcgccgggc gtgatctggc gcaagaaggt
     3061 ggcgcagccc gtgcagaccg ggctgaaatc gatcgatgcg atggtgccca tcggccgtgg
     3121 ccagcgcgag ctcatcatcg gcgatcgcca gaccggcaag acggcggttg cgatcga
//
LOCUS       DASRWS010000152         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2933514, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000152 DASRWS010000000
VERSION     DASRWS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2933514"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFF72_02760"
     CDS             <1..373
                     /locus_tag="VFF72_02760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZV92108.1"
                     /translation="KGGAFPAILHCYTGGPQLAKRAVELGLHVSFTGAVTFKKSEALR
                     DIAKAVPLDRLLVETDAPFLAPEPYRGKTNEPSYVVHTAATLAQVKGVSPAELAAATT
                     DNFFRLFRKVKRPAERGTSAA"
     gene            370..1167
                     /locus_tag="VFF72_02765"
     CDS             370..1167
                     /locus_tag="VFF72_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV92109.1"
                     /translation="MSYRATILGCGSSGGVPRIGTMWGACDPSNPKNRRRRCSALIER
                     MGKPRHTTVLIDTPPDLREQLLSVRCDALDGILYTHDHADHTHGIDDLRMVSYAMKKR
                     LDVWLDEPTQQSLIQRFAYCFEKPPESTYPPILSAHPIEPPASIRVEGPSGAIEFVPI
                     PQLHGDIGTLGYRVGGIAYSPDISDIPVASLPLLQNLDVWIVDALRFTPHPSHFSVKQ
                     ALEWIKRLRPKRAILTHMTTDLDYEALRRELPEGVEPAYDNMVIEFD"
     gene            complement(1174..1920)
                     /locus_tag="VFF72_02770"
     CDS             complement(1174..1920)
                     /locus_tag="VFF72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV92110.1"
                     /translation="MKNKKPLKDRLALVTGASRGIGRAVALALAQNGAHVVVAARTIG
                     ALEELDDEIRKGGGKATLLQMDLSKGEKIDQLGPTLFQRWGKLDIFVGNAGILGPLSP
                     LHHVTEDAWNSVIDINLSANWRLIRTLDPLLKRAEAGRAVFLTSAAATGKYAYWGPYA
                     VSKAGLEALVKTYAHELADTPVRVNLVNPGPVRTAMRAKAFPGEDPSTLPAPEELVPM
                     FLEFASPTCELNGRVVNFSDWQKKHAPTDT"
BASE COUNT          362 a          718 c          653 g          371 t
ORIGIN      
        1 caagggcggc gccttcccgg ccatcctgca ctgctatacc ggcggcccgc agcttgccaa
       61 acgtgctgtc gagctcggcc tccacgtgtc gttcaccggt gccgtcactt tcaagaagtc
      121 ggaggcgttg cgggatattg ccaaggcggt gccgctcgac cggctgctgg tcgagacgga
      181 tgcgccgttc ctggcgcccg agccctaccg cggcaagacg aatgagccat cttatgtcgt
      241 gcatacggcg gcgacgctcg ctcaggtgaa gggcgtgtca ccggccgagc tcgctgcggc
      301 gacgaccgac aacttcttcc gcctgttccg caaggtgaag cgtccggccg agcgtggtac
      361 ctctgccgca tgagctaccg cgccacgatc ctcggctgcg gctcttcggg cggcgtgccg
      421 cgcatcggga cgatgtgggg cgcctgcgat ccgtcgaacc cgaagaaccg ccggcggcgc
      481 tgcagcgcgc tcatcgagcg catgggcaaa ccgcgccaca cgacggtgct gatcgacacc
      541 ccgcccgacc tgcgcgagca gctactcagc gtgcgctgcg acgcgctcga cggcatcctc
      601 tacacgcacg accacgccga ccacacgcac ggcatcgacg atctacgcat ggtctcctac
      661 gccatgaaga agcgccttga tgtttggctc gacgagccga cgcagcaaag cctcatccag
      721 cgcttcgcct attgcttcga gaagccgccg gagtcgactt acccgccgat cctcagcgcg
      781 catccaatcg agccgccggc gtcaatccgc gtcgagggcc catccggcgc catcgagttc
      841 gtacccatcc cacaattgca cggcgacatc ggcacgctcg gctatcgcgt cggcgggatc
      901 gcctattcgc cggacatcag cgacatcccc gtcgcttcgt tgccgctgct gcagaacctc
      961 gacgtgtgga tcgtcgatgc cctgcgcttc accccccacc cgagccactt cagcgtcaag
     1021 caggcgctcg aatggatcaa gcgcttgcgc cccaagcgcg ccatcctcac gcacatgacg
     1081 acggatctcg actacgaggc gttgcggcgc gagctgcccg agggtgtcga gccggcctac
     1141 gacaacatgg tcatcgagtt cgattgagcg tgtttaggtg tcggtcggcg cgtgcttctt
     1201 ctgccagtcg ctgaaattga caacgcgtcc attcagctcg caagtgggcg aagcgaactc
     1261 gaggaacata ggcaccaact cttccggtgc cggcagcgtg cttggatctt cgccgggaaa
     1321 cgccttggcg cgcatcgccg tgcgtaccgg ccccgggttg acgaggttga cgcgcaccgg
     1381 ggtatccgcg agctcatggg cgtaggtctt caccagcgcc tcgagtccgg ccttcgacac
     1441 cgcgtacgga ccccagtagg catacttgcc cgttgctgca gccgacgtga ggaacaccgc
     1501 gcgccccgct tccgcgcgct ttagcagcgg atcgagcgtg cggatcagcc gccagttggc
     1561 cgaaaggttg atatcgatca ccgagttcca ggcatcctcg gtgacgtggt gcagcggcga
     1621 cagcgggccg aggatgccgg cattgccaac gaagatgtcg agcttgcccc agcgctggaa
     1681 cagcgtcggc ccgagttgat cgatcttctc gcccttggag aggtccatct gcagcagcgt
     1741 cgccttgccg ccgcccttgc ggatttcgtc gtcgagctcc tccagcgcgc cgatggtgcg
     1801 cgcggcgacg acgacatgcg cgccgttttg agcgagagcc aaagctaccg cgcgtccgat
     1861 gccgcgcgag gcgccggtga cgagtgccag ccggtctttg agcggtttct tattcttcat
     1921 gaccacgcac agcctggcgc aaaaatgcct cgcctgctct gacagctaaa ggtcatgagc
     1981 ggacgaggca agccctacca gggtcgtaga tcggcctcgg ctggttaacc gacttcggcc
     2041 agcaacgaca gctgacgcgg catcgtgtgc ccgctctgat cggtgagagg tgtcggatat
     2101 tcac
//
LOCUS       DASRWS010000153         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_55552, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000153 DASRWS010000000
VERSION     DASRWS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55552"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VFF72_02775"
     CDS             complement(<1..98)
                     /locus_tag="VFF72_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZV92111.1"
                     /translation="MKFTAYERTQQGTGASRRLRNAQKVPGIVYGAG"
     gene            complement(201..1163)
                     /locus_tag="VFF72_02780"
     CDS             complement(201..1163)
                     /locus_tag="VFF72_02780"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZV92112.1"
                     /translation="MLFNTVLFTGNANPTLAQDIATHLSINLGKAKVARFSDGEVDVE
                     IEQNVRARDIFVVQSTCSPTNENLMELCIMVDALKRASARRVTAVIPYFGYARQDRRP
                     RSTRVPISAKVVANLLEAVGVERLLTMDLHADQIQGFFNIPVDNIYASPVLLSDLQSK
                     RYEDLVVVSPDVGGVVRARALAKQAGCDLAIIDKRRPRANVSEVMHVIGDIEGRNCVI
                     MDDMIDTAGTLVKAADVLKERGAKKVYAYCTHAVFSGPAIERIQSSKLDEVVVTDTIP
                     LGEAGRKSAKVRQLSVAFLFAETIRRISDGESVTSLFAEQNNNF"
     gene            complement(1202..1278)
                     /locus_tag="VFF72_02785"
     tRNA            complement(1202..1278)
                     /locus_tag="VFF72_02785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1242..1244),aa:Gln,seq:ttg)
     gene            complement(1341..>2224)
                     /gene="ispE"
                     /locus_tag="VFF72_02790"
     CDS             complement(1341..>2224)
                     /gene="ispE"
                     /locus_tag="VFF72_02790"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZV92113.1"
                     /translation="NLFLHVTGRRDDGYHLIDSLMVPIDWCDTLHFERRDDGRLVRHD
                     LGAALPADDLCLGAATALQRASKTPLGVDISIDKSVPWGAGLGGGSSDAASTLLALNR
                     LWALDWPLERILKLGLSLGADVPFFLGATPARVGGIGERLEPVPFAARRFAVVKPAAN
                     LDTRSVFASDRVARTAEAARIAGFPEAAHRPQDGPAQSRESGPEIAEGFGRNDLQAAA
                     EELCPDVARAAAGLRAAFGNSRMTGSGSAVFAWVAEAVDGSSAAAMPADWPVGWSGRM
                     CRSLERHPLDGWARRES"
BASE COUNT          361 a          753 c          755 g          355 t
ORIGIN      
        1 cccgcgccgt agacgatgcc agggaccttt tgcgcgttgc gcaggcggcg gctcgctccg
       61 gtcccctgct gcgtgcgctc gtaagcggtg aatttcattt cgactccaaa agatgaggcc
      121 cgcgaccagg ctctcgggaa aaacggagcg acgctccgca actcgcaggg ggttcgccag
      181 ggcgagcccc cgatgtcctt ctagaaattg ttgttctgct cggcgaacag cgacgtcacc
      241 gattccccgt cggagatgcg gcggatcgtc tcggcaaaga ggaacgccac cgacagctgg
      301 cgaaccttcg cactcttgcg gccggcctcg ccgaggggga tcgtgtcggt gacgacgacc
      361 tcgtcgagct tcgagctctg gatgcgttcg atcgccgggc ccgagaacac cgcatgcgtg
      421 cagtacgcgt agaccttctt cgcgccgcgc tccttcagca cgtcggccgc cttcacgagc
      481 gtgccggcgg tgtcgatcat gtcgtccatg atcacgcagt tgcggccttc gatgtcgccg
      541 atgacgtgca tcacctcgga gacgttggcc cggggccggc gcttgtcgat gatcgccagg
      601 tcgcagcccg cctgcttggc gagcgcccgc gcgcgcacga cgccgccgac gtcgggcgag
      661 acgacgacca ggtcttcgta gcgcttgctc tgcaggtcgg acagaaggac cggcgaggcg
      721 tagatgttgt cgaccgggat gttgaagaag ccctggatct ggtcggcgtg caggtccatg
      781 gtgagcagcc gctcgacgcc gaccgcctcg agcaggttgg cgacgacctt ggcggaaatc
      841 ggcacccgcg tcgagcgtgg ccggcgatcc tggcgggcgt agccgaagta gggaatcacg
      901 gccgtcaccc gtcgcgccga ggcgcgcttg agcgcatcga ccatgatgca cagctccatc
      961 aggttctcgt tggtcggcga gcacgtcgac tggacgacga agatgtcgcg ggcgcgcacg
     1021 ttctgctcga tctcgacgtc gacctcgccg tcggagaagc gcgcgacctt ggccttgccg
     1081 agattgatcg acaggtgcgt ggcgatgtct tgcgccagcg tcgggttggc gttgcccgtg
     1141 aacagaacgg tgttgaacag caaggcgact ttcccggatc gagcgtttgc gctagcggat
     1201 ttggcagggg aggaaggact cgaaccctcg catgtcggaa tcaaaatccg atgccttgac
     1261 caacttggcg actcccctac accggtccgc ggccttcgcc gcgaaccttc aaccgtgcgc
     1321 gacccgcgtc gcgacgttcc tcaactttcc cgcctcgccc atccgtcgag cggatggcgc
     1381 tccagcgagc ggcacatccg gcccgaccag ccgacgggcc agtcggccgg catcgctgcc
     1441 gcgctcgagc catcgaccgc ctcggcgacc caggcgaaga ccgcgcttcc cgaacccgtc
     1501 atccggctgt tgccgaatgc ggcgcgaagc ccggccgccg cgcgagcgac atcggggcaa
     1561 agttcctcgg cggctgcctg cagatcgttg cgcccgaagc cttcggcaat ttccggcccg
     1621 gattccctcg actgagcagg cccgtcttgc gggcgatgcg cggcctcggg aaagcccgcg
     1681 attctagcag cctcggccgt tcgagcgacc cggtcgctgg cgaaaacgga gcgggtgtcg
     1741 aggttcgccg ccggcttgac gacggcgaat cgccgcgccg cgaagggcac cggctcgagc
     1801 cgctcgccga tgccgccgac gcgcgccggg gtggcgccga gaaagaacgg cacgtcggcg
     1861 cccagcgaca ggccgagctt caggattcgc tcgagcggcc agtcgagcgc ccagagccgg
     1921 ttgagggcca acagcgtcga cgcggcgtcg gagctgcctc cgcccagccc ggcgccccac
     1981 ggcacggact tgtcgatcga gatgtcgacg cccagcggcg tcttcgaagc gcgctgcagc
     2041 gcggtcgcgg cgcccaggca caggtcgtcg gcgggaagcg ctgcgcccag atcgtgacgg
     2101 accagcctgc catcgtcgcg gcgctcgaaa tgaagcgtgt cgcaccagtc gatcggcacc
     2161 atcagcgagt cgatgaggtg gtagccgtcg tcgcgccgcc cggtcacgtg caggaacaga
     2221 ttga
//
LOCUS       DASRWS010000154         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2075080, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000154 DASRWS010000000
VERSION     DASRWS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075080"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFF72_02795"
     CDS             complement(<1..1191)
                     /locus_tag="VFF72_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZV92114.1"
                     /translation="MRTSRGTAARPQMAAASLAGAAAVVLALAGCAVGPDFKTPAAPV
                     VADAAHPYTPVPMPAQTASAPVSGGAAQRLALGQDIPALWWDLFRSQPLDDLVRSALT
                     QSPTLASAQAALRQAQANYEADSGRKLEPSLTGSLGAEREQVSQVASGIPGGALYNLY
                     NASVNISYNIDAFGATRRELEGLQSAIDYQRYQVEATYLSLTTNLVTTAIQEASLRAQ
                     LKATRDVVEVQQKSLDLVQKQAQLGAIARGIVLSQQALLAQTRASLPGLEKALEQTRH
                     QLAVYAGRLPGDPGLPQFDLDTLHLPEELPVSLPSALVRQRPDVRAAEALLHQASAQI
                     GVATANLYPQITLSGSFGSQALKVDKLFSNGSLAWSAGAGLLQPIFNGGALQASKRAA
                     IAAYD"
     gene            complement(1188..2003)
                     /locus_tag="VFF72_02800"
     CDS             complement(1188..2003)
                     /locus_tag="VFF72_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HZV92115.1"
                     /translation="MNSDLPDDGLCVVAQSFAGLRDPGEPARERLLLAALRLFAEQGY
                     AKTSIRQIAQAAGANVAAVSYYFGNKAGLYRAVFWGGATPPPDPAAQVRAGAGSLESL
                     FEHILAPLRNGDKARLWIKLHRREMIEPAGLWREKVDRGMQPMHAALVALLCRRLGVD
                     RADDEVLALATLVIGPAVHLLVNCEVVDALAPQLLAAPDSVDVWRGRLVRSAETLIAA
                     ERRRRVAAARTKARKSRGTGPRAMPGAASATRKQATTVKKRSASQATRTRSRT"
     gene            complement(2058..2585)
                     /locus_tag="VFF72_02805"
     CDS             complement(2058..2585)
                     /locus_tag="VFF72_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZV92116.1"
                     /translation="MKHCRTCGGEVRYVTPEGDNRERATCSACATIHYENPLNVVGTL
                     PFWGEEVLLCRRNIEPRRGLWTLPAGFMELGESTAEGALRETEEEAGAHVELLDLFSL
                     LNVARVGQVHLFYRARMTDADFEPGPETIEAKLFGEADIPWDEIAFRTTRKTLELFFA
                     DRRAGAYGFHCVDIA"
BASE COUNT          366 a          870 c          949 g          403 t
ORIGIN      
        1 atcgtaggca gcgatcgcgg cacgcttgct cgcctgcagc gcgccgccat tgaagatggg
       61 ctgcaacagg ccggcgccag cgctccaggc caggctgccg ttcgagaaca gcttgtcgac
      121 cttgagcgcc tgcgagccga agcttccgct cagggtgatc tgcgggtaga ggttggcggt
      181 cgccacgccg atctgcgcgc tggcctggtg caggagtgcc tcggcggcgc gcacgtccgg
      241 gcgctgccgg accagtgccg aaggcaggct caccggcagt tcctcgggca ggtgcagggt
      301 atcgaggtcg aactgcggca ggcccgggtc gccgggaagc cggccggcat agacggcgag
      361 ctggtggcgg gtctgctcga gcgccttctc gagcccgggc aggctcgccc gcgtctgcgc
      421 cagcagcgcc tgctgcgaga ggacgatgcc gcgcgcgatc gcgccgagct gcgcctgctt
      481 ctgcaccagg tcgagcgatt tctgctgcac ctcgacgacg tcgcgcgtcg ccttcaattg
      541 cgcgcgcagc gacgcctcct ggatcgccgt cgtgacgaga ttggtggtca ggctgaggta
      601 ggtcgcctcg acctggtagc gctggtagtc gatcgccgac tgcaggccct cgagctcgcg
      661 tcgcgtcgca ccgaaggcgt cgatgttgta ggagatgttg accgaggcgt tgtagaggtt
      721 gtagagcgcg cctccgggaa tgccgctggc gacctgcgac acctgctcgc gctcggcgcc
      781 gagcgatccg gtcagcgaag gctcgagctt gcggccggag tcggcttcgt agttcgcctg
      841 ggcctggcgc agcgccgcct gggccgaggc cagggtcggg ctttgcgtca gggcggagcg
      901 aacgaggtcg tcgagcggct gcgagcgaaa cagatcccac cagagcgccg ggatgtcctg
      961 gccgagggcc aatcgctgcg cggcaccgcc cgataccggc gcgctggccg tctgcgccgg
     1021 catcggcacc ggcgtatacg ggtgcgcggc gtcggcgacg accggcgcgg ccggggtctt
     1081 gaagtcgggg ccgaccgcgc agccggccag agcgagcacg acggccgctg caccggccag
     1141 gctcgcggcc gccatctgcg ggcgcgccgc cgtgcctcgt gaagtcctca tgtcctgctc
     1201 ctcgtgcgcg tggcttgcga agccgatcgc ttcttcaccg tcgttgcctg tttccgggtc
     1261 gcgcttgccg cgcccggcat ggcgcgaggc ccggtaccgc gactcttgcg cgctttcgtc
     1321 cgggcggcgg cgacgcgtcg cctgcgctcg gcggcgatca gcgtctcggc actgcgaacc
     1381 agccggccgc gccagacatc gaccgagtcc ggcgctgcca gcagttgcgg cgcgagggcg
     1441 tcgaccactt cgcagttgac gagcaggtgc acggccgggc cgatgacgag cgtggccagc
     1501 gccagcacct cgtcgtcggc cctgtcgacg ccgagtcgac ggcagagcag agcgaccagc
     1561 gcggcgtgca tgggctgcat gccgcggtcg accttctcgc gccacaggcc ggccggctcg
     1621 atcatctcgc gccgatgcag cttgatccag agcctggcct tgtcgccgtt gcgaagcggc
     1681 gccaggatgt gttcgaagag gctttcgagc gacccggcac cggcgcggac ttgcgcggcg
     1741 gggtcaggcg gcggcgtcgc accgccccag aaaacggcgc ggtaaaggcc ggccttgttg
     1801 ccgaagtaat agctgacagc ggcgacgttg gcgcccgctg cctgggcgat ctgccggatc
     1861 gacgtcttgg cgtagccctg ctcggcgaac aggcgaaggg ccgccagcag caggcgctcg
     1921 cgggcgggct cgccggggtc gcgcagaccg gcgaacgatt gggcgacgac gcacaggccg
     1981 tcgtcgggca ggtcggaatt catcgccgcg cattctagcc acggcccgaa acaatcgttt
     2041 gattgagacg gcgacggtca ggcgatgtcg acgcagtgga aaccgtaggc gccggcgcgc
     2101 cggtcggcga agaagagctc gagcgtcttg cgggtcgttc gaaaggcgat ctcgtcccag
     2161 gggatgtcgg cctcgccgaa cagcttcgcc tcgatcgttt ccggtccggg ctcgaagtcg
     2221 gcgtcggtca tgcgggcccg gtagaaaaga tgcacctggc cgacccgcgc gacgttgagc
     2281 aggctgaaga ggtcgagcag ctcgacatgc gcgcctgcct cctcttccgt ctcgcgaagc
     2341 gcaccctcgg cggtgctttc gccgagctcc atgaagcccg ccggcagcgt ccacaggccc
     2401 cgccggggct cgatgttgcg ccggcacagc aacacctctt cgccccagaa gggcagcgtg
     2461 ccgacgacgt tgagcgggtt ctcgtagtgg atcgtggcgc aagcggagca ggttgcccgc
     2521 tcgcggttgt cgccttccgg cgtgacgtag cggacctcgc cgccgcaggt ccggcaatgc
     2581 ttgatgct
//
LOCUS       DASRWS010000155         5933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3802816, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000155 DASRWS010000000
VERSION     DASRWS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802816"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..832
                     /locus_tag="VFF72_02810"
     CDS             206..832
                     /locus_tag="VFF72_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV92117.1"
                     /translation="MVADRADGATTRASILRAARDRLVASGYANLNVRDIAREAGVNH
                     ALIGYHFGGKQQLVLAVLDEANKQLIARQTSMYEGTPKASEQWQQACDFYKVDLDSGF
                     VRLLMELMGASFHDEALRREFVPRLTQWHRLVEAAVLEFIARSKLQLPISAQAISAWI
                     VWFWTGMQAGTMLGIKEKDGHQREALEAMATLLRLVEGSAKTRVRKRP"
     gene            832..3000
                     /locus_tag="VFF72_02815"
     CDS             832..3000
                     /locus_tag="VFF72_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV92118.1"
                     /translation="MDTRPVSELPRRLALTAGPLPEQGTLPHPYPYESIAAVREGFVE
                     RGGVRSWYAQFGESGPWLAFAPVFQIANANMLKGVVPWLAQHFRVVVGDLRGNGRSDR
                     PTLPEQYGFDHYFADFVTVLDLLEVDHVALVGISAATMTVLRLAAEQPERITHLVIAG
                     GFAARLIEGEAAAKDARSVVERMCSDWPAYLDEFFGIVFSEPHSTKPFEDGVRNSAST
                     TGSIVAMGLLGWFGTDMRDNAKKVRCPALVIHGDDDGRVLYANGQQIAALIPGARLLT
                     VRGGGHLLPARDPVAFARIVRDFAGVPPLRSTWVRAMARKRRALFVSSAIGLGHVQRD
                     LAIAREMRKLHPDLEIDWFTVHPASTYLEREGERLHPITIRLANESRHFEARAGEHDL
                     QAFFALRTMDEVMARNFLVFAELLEQEHYDIVIGDEAWEVDYHYHENPELKRQPFVFL
                     TDFVGCLPMEEGNEREAFLCADRNADDIEHVARYPWVRDRAIFVGNPEDVSEQAFGPG
                     LPQIRDWTKRNFAFTGYALPFDPAQLADTEALRARHGYGKDQKLVIAAVGGTAVGLPL
                     LRRIADAFPLMKRQVPELRMVLVAGPRVPRSALPEHAGLDVLPYVHNLFEHLACSDLA
                     LVQGGLSTCMELVATRRRFLSFPLERHFEQCIHVRNRLHNYCADCSVRFRELATDELA
                     ERALAAMHAPVNYRPVETDGAVRAARQILAVMENRSWASA"
     gene            3161..4237
                     /locus_tag="VFF72_02820"
     CDS             3161..4237
                     /locus_tag="VFF72_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV92119.1"
                     /translation="MSTDSLRQPQIRFARSSDGTRIAYAVSGEGPPLVRAAHWLGNLD
                     FDWLTPVWGPWIGALSERHRLLRYDSRGCGLSDREAEAITIDGLVADLECVVDNAGLD
                     RFALLGMSQGGAVSIVYAARHPERVSHLVLCGAFARGPLCRNPTPQQVEATEAMVKLV
                     EFGWGQSNPAFLQMFTSQFFPQATLAQAHSFNEIQRHAASPRVAARLIRAFAVLDASA
                     ELAQVRAPTLVFHSRGDNRVPFEEGRFVATSIAGARFEPLDSNSHVPLPGELAFQRVL
                     DDMAAFVSPVGREAAPFAELTRREREVLDRIARGLDNAQIAAHLGLAEKTVRNHITAI
                     FEKMAVENRAQAIVAARVAGLGSV"
     gene            4402..4635
                     /locus_tag="VFF72_02825"
     CDS             4402..4635
                     /locus_tag="VFF72_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HZV92120.1"
                     /translation="MPFINVKLIEGVFSSEQKQQIVRKLTDAMVSIEGEAMRGVTWAV
                     IEEVKSGDWAIGGQPLTTADVKALAAARPAMAA"
     gene            complement(4886..4961)
                     /locus_tag="VFF72_02830"
     tRNA            complement(4886..4961)
                     /locus_tag="VFF72_02830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4926..4928),aa:Ala,seq:cgc)
     gene            5142..5561
                     /locus_tag="VFF72_02835"
     CDS             5142..5561
                     /locus_tag="VFF72_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZV92121.1"
                     /translation="MSLKGSKTEQSLRDAFAGESQANRRYLYFANKADIEGQNDVAAL
                     FRSTAEGETGHAHGHLEFLEQVGDPATGLPIGSSRLNLKAAVAGETHEYTDMYPGMAK
                     TAREEGFDEVADWFETLAKAERSHANRYQKALDALVD"
     gene            5638..>5933
                     /locus_tag="VFF72_02840"
     CDS             5638..>5933
                     /locus_tag="VFF72_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HZV92122.1"
                     /translation="MTLREGNLEAPTRHPIDWKGPDYHDEDKAFAEMERVFDICHGCR
                     RCVSLCQSFPNLFDLVDATADGEVHGVDKQDYWKVVDQCYLCDLCFMTKCPYVSP"
BASE COUNT         1005 a         1997 c         1934 g          997 t
ORIGIN      
        1 atggccgacc ctcgcgatgc gccgaacacg acgaccttgt cagcgcggca gcgtcgtcca
       61 ccgcctccag atcgaccgcc gcctgaccaa gggttgtcgc caggcatccc ttgacacatt
      121 ccttgggttc gtataaattg aacaaatgga taaaccagtt gatcaatcta ctcgtgccgg
      181 ccgcgttcca aagcagcaga gcacgttggt tgccgaccgc gctgacggcg cgaccacccg
      241 tgcctcgatc cttcgtgcgg cgcgtgacag gctcgtcgcg tcgggctacg ccaacctcaa
      301 cgtgcgcgac atcgctcgcg aggccggtgt gaatcacgcg ctgatcggct accacttcgg
      361 tggcaagcag cagctcgtcc ttgccgtgct cgatgaggcc aacaagcagc tcatcgcgcg
      421 gcagacaagc atgtacgaag ggacaccgaa ggccagtgag caatggcagc aggcctgcga
      481 tttctacaag gtggacctcg attcaggctt cgtgcgcctg ctgatggagc tgatgggcgc
      541 gagttttcat gatgaggcgt tgcgtcggga gttcgtgccg cgattgacgc agtggcaccg
      601 cctggtcgag gccgcggtgc tcgagttcat cgcccgctcc aagctccagc tgccgatatc
      661 ggctcaggcg atttcggcct ggatcgtctg gttctggacc ggcatgcagg cgggcacgat
      721 gctcggcatc aaagagaaag acggccatca gcgtgaggcg ctcgaggcca tggccacgct
      781 gctgcgtctc gtggaagggt ccgccaagac tcgcgttcgc aagaggccct gatggacaca
      841 agaccggttt ccgaattgcc acggcgcctc gcgttgacgg ctggtccgtt gccagagcaa
      901 ggcacgctgc cccatccgta tccgtacgag agcatcgcag cggttcgcga agggttcgtc
      961 gagcgcggtg gcgttcgctc ctggtacgcg caattcggcg agagcggacc ctggctcgcc
     1021 ttcgcgccgg tgttccagat cgccaacgcg aacatgttga aaggcgtcgt gccctggctg
     1081 gcccagcact tccgcgtcgt cgtcggcgat ctgcgcggca acggtcgctc cgaccggccc
     1141 acgttgcccg aacagtacgg gttcgatcac tacttcgccg acttcgtcac ggtgctcgac
     1201 ctcctggagg tcgaccacgt cgcgctcgtc ggcatctctg ccgcgaccat gaccgtgctg
     1261 aggctggcgg ccgagcagcc cgagcggatc acccatctgg tgattgccgg cggcttcgcg
     1321 gcacgactga tcgagggcga ggccgctgcc aaggatgccc gcagcgtcgt cgagcgcatg
     1381 tgcagcgatt ggccggctta cctcgacgag ttcttcggca tcgtcttcag cgagccccat
     1441 tcgaccaaac ccttcgaaga cggcgtgcgc aacagcgcga gcacgaccgg ttcgatcgtg
     1501 gcgatgggcc tcttggggtg gttcggcacg gatatgcgag acaacgccaa gaaggtccgc
     1561 tgcccggcgc tcgtcatcca cggcgacgac gacggccgcg tcctctacgc gaacggccag
     1621 cagatcgctg ccctgatccc gggcgcgaga ctgctgaccg tgcgcggcgg gggccacctt
     1681 ctgccggcgc gcgacccggt cgcgtttgcc cgcatcgtgc gcgattttgc cggcgtaccg
     1741 ccgttgcgct cgacctgggt ccgcgccatg gcaaggaagc gccgcgccct gttcgtatcg
     1801 agcgcgatcg gcctcggcca cgtccagcgc gacctggcca tcgcgcgcga gatgcgcaag
     1861 ctccatccgg acctcgagat cgattggttc accgtccacc ccgcgtcgac ctatctagag
     1921 cgcgagggtg aaagactcca tccgatcacg atccgtctcg ccaacgaaag ccgccatttc
     1981 gaagcccggg cgggcgagca tgacctgcag gccttcttcg cgctgcgcac gatggacgag
     2041 gtcatggcgc gcaactttct cgtcttcgcc gagctgctcg agcaggagca ctacgacatc
     2101 gtcatcggcg atgaagcctg ggaggtcgac taccactatc acgagaatcc ggagctcaag
     2161 cgccagccgt tcgtcttcct gaccgacttc gtcggttgcc tgccgatgga agagggcaac
     2221 gagcgcgagg cgtttctctg tgccgatcgc aatgccgacg acatcgagca cgtcgcgcgc
     2281 tacccgtggg tacgcgaccg tgcgatcttc gtcggcaatc cggaggacgt ttccgagcaa
     2341 gccttcggcc cgggtctgcc gcagatccgt gactggacga agcgcaactt tgcctttact
     2401 ggctacgcct tgccgttcga cccggcgcaa ttggccgaca ccgaggcctt gcgggcccgc
     2461 cacggctacg gaaaggacca gaagctggtc atcgccgccg tcggggggac ggccgtcggc
     2521 ctgcccttgc tgcgccggat cgccgatgcg ttcccgctca tgaaacgcca ggtgcccgag
     2581 cttcgcatgg tgctggtcgc tggcccacgc gtgccgcggt cggcgctgcc ggagcacgcc
     2641 gggctcgacg tgctgcccta cgtgcataac ctgttcgagc atctcgcttg ctcggacctt
     2701 gccctggttc agggcggctt gtcgacctgc atggaactcg tcgcgacgcg caggcgcttc
     2761 ctcagctttc cgctagaacg ccacttcgag cagtgcatcc acgtgcgcaa ccgacttcac
     2821 aactactgtg ccgactgttc ggtaaggttt cgcgagctgg cgacggatga actcgcagaa
     2881 cgcgccctgg cggcgatgca cgcacccgtc aattaccggc cggtcgagac cgacggcgcc
     2941 gtgcgcgcgg cgcgacagat cctggcggtc atggagaacc gctcgtgggc atcggcgtga
     3001 cgatcacggc cccaacgcgg acgagtcgtc cgcgacccga acaaatcgct ccggtgactt
     3061 ctcgatcggc acggcgacag gcgcctgccg agggccagga catccgccat cgtcgcgacg
     3121 aagagcctcg gcgccagcgg tcgccgcgca caatgtcagc atgagcacag attccctgcg
     3181 ccagccgcag atccggtttg ctcgctcgtc cgatggcacc aggatcgcct atgccgtcag
     3241 tggcgaaggg ccgccactgg tccgcgccgc gcactggctg ggcaacctcg acttcgactg
     3301 gctgacgccc gtctggggtc cgtggatcgg tgcgttgagc gagcgccatc ggttgctgcg
     3361 ctacgactcg cgtggctgcg gtctgtccga ccgcgaggcg gaggccatca cgatcgacgg
     3421 cctggtcgcc gatctcgagt gcgttgtcga caacgccggg ctcgatcgct tcgccttgct
     3481 cggaatgtcg caaggcggcg cggtgtcgat cgtctacgct gcgcgccatc ccgagcgggt
     3541 cagccatctc gtgctctgcg gcgcgttcgc gcgtgggccc ttgtgccgca atccgacgcc
     3601 gcagcaggtc gaggcgaccg aggccatggt caagctcgtc gaattcggtt gggggcagag
     3661 caacccggcc tttctgcaga tgttcacgtc gcagttcttt ccgcaggcga cgctggccca
     3721 ggcgcattcg ttcaacgaga tccagcgcca cgcggcctcg ccgcgcgtgg cggcgcggct
     3781 catccgcgcc tttgccgtgc tcgacgcgtc ggcggagctg gcgcaggtgc gggcgccgac
     3841 gctcgtgttc cacagccggg gcgacaaccg ggttccgttc gaggagggcc gtttcgtcgc
     3901 gacctcgatt gccggagcgc gcttcgagcc gctcgacagc aacagccacg tgccgctgcc
     3961 gggcgagctc gcctttcagc gcgtgctcga cgacatggcg gccttcgtgt cgcccgtggg
     4021 ccgggaagct gctccgttcg ccgagctgac gcgtcgggag cgcgaagtgc tggatcggat
     4081 cgcccgcggg ctcgacaacg cgcagatcgc cgcccatctg ggcctggcgg aaaagacggt
     4141 gcgcaaccac atcaccgcca tcttcgaaaa gatggcggtc gaaaaccgcg cgcaggcgat
     4201 cgtggccgcg cgtgtcgccg gcctcggcag cgtctgaggc ggccagggcc gcagacctac
     4261 gggcgaggag actttcgacg gccgatcacg ccaaaccggg acctcggccc cgggacgctg
     4321 cgccgatctg ctggcccact gcttctgccg gcaagacacc acgcacacgc cgcggtgccg
     4381 tccggagcag aggagcacac catgcctttc atcaacgtca agctcatcga gggcgttttc
     4441 tcgtccgagc agaagcaaca gatcgtccgc aagctcaccg acgcgatggt cagcatcgaa
     4501 ggtgaggcca tgcgaggcgt cacctgggcc gtgatcgagg aagtcaagag cggcgactgg
     4561 gcgatcggtg gccagccact gacgaccgct gacgtcaagg ccctggccgc ggccaggcca
     4621 gcgatggccg cgtagcctcc cctcgaagag gttcgtggga ccaggccggc gcgacggtgt
     4681 gaagggggca gcacatgaac accggagaag cgcacgacta cgtcgtcgtg ggcgcccgcg
     4741 ccgccctgaa ggccggcggg tgccgctttg ccggagcaac tggtcgcgcg ccggcacctc
     4801 gggcatcgcc ctgtccactg ggaatgcgat ctccgatttg tcgaagcctc ccgggattgc
     4861 tcgggatctt gctggacttg ccggatggtg gagaggagga ggatcgaact cccgaccttc
     4921 gcattgcgaa cgcgacgctc tcccagctga gctacccccc cacggcgacc ggcagtttag
     4981 caaactggcc agagcgttcc ccacctggcg cgtacggata gcccgaccca atactgcgga
     5041 ttgaagggct ttattgcctc gcggctcgcg acgcgctagg gtcggcaggt cgctcgccgt
     5101 tgccggccgc gttcacccac agacactttc aggagaccgt catgagtttg aagggatcga
     5161 aaaccgagca gagcctgcgc gatgcgttcg ccggcgagtc gcaggccaac cgccgctacc
     5221 tctatttcgc gaacaaggcc gacatcgagg gccagaacga cgtcgcggcg ttgtttcgct
     5281 cgaccgccga gggcgagacg ggccatgccc acggtcacct cgagttcctc gagcaggtcg
     5341 gcgacccggc caccggtttg ccgatcggct cgagccggct caacctgaag gccgccgtcg
     5401 ccggcgagac gcacgagtac accgacatgt accccggcat ggccaagacg gcccgcgagg
     5461 aaggcttcga cgaagtggcc gactggttcg agacgctggc caaggccgag cgctcgcacg
     5521 ccaatcgcta ccagaaggcg ctggacgcgc tggtcgactg atcggcagcg atcacgctcg
     5581 atcacacaac aagggccgtc gcggcccttg ttcgttgatg gcatacagga accgacgatg
     5641 accctgcgcg aaggaaacct cgaagcgccg acccggcacc cgatcgactg gaaagggccc
     5701 gactatcacg acgaggacaa ggccttcgcc gagatggagc gcgtcttcga catctgccac
     5761 ggctgccggc gctgcgtgag cctgtgccag tcgttcccga acctgttcga tctcgtcgac
     5821 gccacggccg acggcgaggt tcacggtgtc gacaagcagg actactggaa ggtcgtcgac
     5881 cagtgctacc tgtgcgatct ctgcttcatg accaagtgcc cgtacgtgtc gcc
//
LOCUS       DASRWS010000156         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_637445, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000156 DASRWS010000000
VERSION     DASRWS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637445"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..895
                     /locus_tag="VFF72_02845"
     CDS             134..895
                     /locus_tag="VFF72_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZV92123.1"
                     /translation="MEIRALTRAARILASISVSPTGQTLSELVASVDLSKATTHRFVQ
                     ALVTIDYLRYDSRSATYHIGPALLGFANAGAGLEELRALARPVLKDLRSATLETISLV
                     VPSGLSRLTVEVEFSEHELRAVPEPGGIKPIHAGAAGKALLAWYPKEALHGLLKDLDL
                     RAVTPQTITTAPELLRALARVRSRGYAISAGETEPGQSASAAPIFGADGTVMGCLNIS
                     GPASRLPAATLRANGELAVAAAKKLGSRLSNGQKT"
     gene            918..1886
                     /locus_tag="VFF72_02850"
     CDS             918..1886
                     /locus_tag="VFF72_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92124.1"
                     /translation="MKSLLAIAILGAAIAAAPAAAADTYPSRVIKIVNPYVTGGTNAF
                     FADLVAKSLSEQLGVSVIVTPQPGAGGNIGSANVARADPDGYTLLAGTSGSNAVNPAL
                     YKNMPYDARRDLRLIATLSSVDNILVVRSDSPYKSVHDIIAEATSSPAKVTYASSGIG
                     SVLHLCGATMASITGTTMTHVPYKGTAPALIDLMGGRVSFMFANAPSVVGQVKAGQLR
                     ALAVTGSQRSSVFPNVPTMQEAGVPGFDLTSWFGVFAPARTPEPIVAKLNLSIRKFLE
                     EPTTKERFASQSATPLIMDQAAAEAFFLKELDSWAQVVKISGAQVD"
     gene            1895..2812
                     /locus_tag="VFF72_02855"
     CDS             1895..2812
                     /locus_tag="VFF72_02855"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV92125.1"
                     /translation="MMRILVTGGAGNIGRDVTRLLRELGHEPVVYDLVAVDDDSGRCI
                     QGDIRDPSSLERAFDEVRPHGVIHLAGTLQFACEADPTGTVSVNVDGTAAVLDAALRA
                     GASRFVLSSSAAVYGSTAETVVETSPVQGDISVYGASKLLAERMLHRYAVLHGMTCRT
                     VRLSTVVSARRVASPGVAAAVRSILDSATGADVAVSGIAGHELRHFVHVSDAAQGAIL
                     ALVAGDDVKGELFNIAGSDDAYLDFDALVALVRRVRPHAGRVSFAGASGHRGRIDCTR
                     AKRELGYRPRYTMQRAIQEAIETCPVNTQ"
     gene            2809..>3153
                     /locus_tag="VFF72_02860"
     CDS             2809..>3153
                     /locus_tag="VFF72_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZV92126.1"
                     /translation="MKAKSDARAQGEVPDELQLEMLEKLVLVREFELACEAWWKAGVH
                     LVGEFHLSLGQEAFAVGTCMAAQPTDPICPSIRGMGVYLCRGAPMERLIASFLERMNG
                     ISDGRWAHWHTPV"
BASE COUNT          576 a         1059 c         1003 g          515 t
ORIGIN      
        1 ttcagcggcg tccgtacgaa aacctcgttc gtagcgtgct gggcgcaggt ctccgaacgt
       61 gccgcagttg ccagaaggcg cgatgatcga tacgataacc aagcaatgga cctgtagttc
      121 cactggctaa aacttggaaa ttcgagccct tactcgcgcc gcgcgaatcc tcgccagcat
      181 cagtgtcagt cccacgggcc aaacactgag cgagctcgtc gcgagcgtgg atctcagcaa
      241 ggcaacgacg catcgatttg tgcaggcgct cgtcaccatc gactacctgc gctacgacag
      301 tcgttcggcg acgtatcaca tcggcccggc gctactcggc ttcgccaacg ccggggccgg
      361 cctggaggaa ctgcgcgcgc tggcgcggcc agttctcaag gatctgcgaa gcgccacgct
      421 cgaaaccata tcgctggtcg tgccgtcggg actcagccgg ttgacggtgg aggtggagtt
      481 cagcgagcac gagctgcgcg ccgtgcctga gccggggggc atcaagccaa tccatgctgg
      541 cgctgcgggc aaggctctgc ttgcctggta tccaaaagaa gcgcttcacg gcctcctcaa
      601 ggaccttgac cttcgagcgg tgacgccgca aacgatcacg accgcgcctg agctattgcg
      661 cgcgctcgcg cgtgtccgct cccgtggata cgcaatcagc gccggcgaaa ccgaacccgg
      721 ccagtcggca agcgcagcgc cgatcttcgg cgccgacggg acggtaatgg gctgcctgaa
      781 catttccggc cccgccagcc ggctcccagc agcgaccctg cgcgccaacg gcgagctcgc
      841 ggtcgccgca gccaagaagc tgggaagccg actgagcaac ggccagaaga catgaacaac
      901 cacaggaagg agacttcatg aagagcttgc ttgcgatcgc catcctcggg gcggccatcg
      961 cggctgcacc ggctgcggcg gcggatacgt acccgagcag ggtgatcaag atcgtgaatc
     1021 cctatgtcac cggcggcacg aacgcgttct tcgccgatct cgtcgccaag tcgctgagcg
     1081 agcagctcgg tgtgagcgtg atcgttacgc cacaaccagg agcaggcgga aatatcggct
     1141 cggccaacgt ggcgagggcc gaccccgacg gatacacatt gctggccggc acgtccggtt
     1201 cgaacgccgt gaatccggcg ctctacaaga acatgccgta cgacgcgaga cgcgatctgc
     1261 gccttatcgc aacgctgtcg agcgtcgaca acatcctcgt cgtgcgcagc gacagccctt
     1321 acaagtcggt gcacgacatc atcgcggaag ccacgtccag cccggcgaaa gtgacgtatg
     1381 cctcctcggg gatcggcagc gtcttgcatc tgtgcggcgc gacgatggcc agcatcacag
     1441 gcaccaccat gacgcatgtg ccctacaagg gcaccgcgcc tgcgctcatc gacctgatgg
     1501 gcggccgggt gagcttcatg ttcgccaacg ccccgtcggt ggtcggccag gtcaaggcgg
     1561 ggcaactacg cgcactcgct gtgaccggct cgcagcgaag ctcggtgttc cccaacgttc
     1621 cgaccatgca ggaggcaggc gtacccggct tcgacctgac ctcgtggttc ggcgtcttcg
     1681 ctccggcgcg cacgcccgag cccatcgtcg ccaaactcaa tctttcgatc cgcaagttcc
     1741 tcgaggagcc gacaaccaag gagcgcttcg ccagccagag cgccacacca ctcatcatgg
     1801 accaagccgc cgccgaagcc ttcttcctga aggagctcga ctcgtgggcc caggtcgtca
     1861 agatctcggg cgcccaagtc gactgaccgc ggcgatgatg cgcatcctcg tcaccggcgg
     1921 cgccggaaac atcggacgcg acgtgacgcg cctgcttcgc gagctaggtc atgagcccgt
     1981 cgtctacgac ttggttgccg tcgacgatga cagcggccgc tgcatccagg gcgacatccg
     2041 cgatccgtca tcgctggagc gcgcgttcga cgaggtgagg ccccacggtg tgatccacct
     2101 tgcgggaacg ctccagttcg cctgcgaagc cgacccgacc ggcacggtct cggtcaacgt
     2161 cgatggcacc gctgctgtgc tggacgcagc actgcgcgct ggcgcgagtc gcttcgtgct
     2221 ctcgagctcg gccgctgtat acggctcgac cgccgagact gtcgtcgaga cgagcccggt
     2281 gcaaggcgat atcagcgtgt atggcgcgag caagctgctc gccgaacgca tgctgcatcg
     2341 ctacgcggta ctgcacggca tgacctgccg caccgtgcgc ctgtcgactg tcgtctcggc
     2401 gcgccgggtg gcgagccccg gcgtcgccgc ggcggtacgc tcgatcctcg actcggccac
     2461 cggcgccgac gtggccgtga gcggcatcgc cggccatgag ctacgtcatt tcgtccatgt
     2521 cagcgacgcg gcgcaagggg cgatcctcgc gctcgtcgcc ggcgacgatg tcaagggcga
     2581 gctcttcaac atcgccggca gcgacgacgc ctacctcgac ttcgacgcgc tggtcgcact
     2641 ggtgcgccgg gttcgtccac acgcaggacg cgtgagcttt gcgggcgcat ccggacaccg
     2701 cggccgcatc gactgcacgc gcgccaagcg cgaattgggc taccgcccgc gatacaccat
     2761 gcaacgtgcc attcaggagg cgatcgagac atgtcccgtc aacacccaat gaaggcgaaa
     2821 tccgacgcac gtgcgcaggg cgaggtgcct gacgagttgc agctcgagat gctcgagaag
     2881 ctcgtgctcg tgcgcgagtt cgagcttgcc tgcgaagcct ggtggaaggc cggcgtgcat
     2941 ctggtcggcg aattccacct gagcctgggc caggaagcct ttgcggtggg aacctgcatg
     3001 gcagcccaac cgacggatcc gatctgcccg tcgattcgcg gcatgggggt ctatctgtgc
     3061 cgcggggctc ccatggagcg gttgatagcc tcctttctcg aacgcatgaa tggaatcagc
     3121 gacggacggt gggcccactg gcacaccccg gtc
//
LOCUS       DASRWS010000157         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3804443, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000157 DASRWS010000000
VERSION     DASRWS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804443"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..2279)
                     /locus_tag="VFF72_02865"
     CDS             complement(156..2279)
                     /locus_tag="VFF72_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZV92127.1"
                     /translation="MPAQAERQLQAKPQPASKSLAPVAGDVTQLAPVVVTATRTESAA
                     FDVPASIDRIDRQTIEDARPMINISESLGAVAGLVARDRQNYAQDVQLSVRGFGARST
                     FGIRGVRLYVDGIPATLPDGQGQISNVDLGSADRIEVLRGPFSALYGNSSGGVVQVFT
                     EEGSGPPTLSSSLVAGSDGMRRESIKARGASGAFGYVVSGSDFSTDGYRQHSAAERRI
                     GNMKLTWRPDDDSKLTLVANSLSLPNADDPLGLTRAQFEADPRGVDPAALAFDTRKTV
                     DQSQLGIVYERRLDAANSLRALVYDGHRNTQQFQSIPVATQTNPLNPGGVIALGRDYR
                     GIDVRWSLRTRLGDAPLGVVGGLAWDTLREARQGFQNFIGPTLGVQGALRRNETNRVV
                     DLDPYVQATLKPSPAWTIDAGVRRSHVRFGSVDHYIVGANPDDSGSVGYAATLPVVGV
                     LFAASDDVHLYATAGRGFETPTLNELAYRPNGATGLDLGLQAATSDSVEAGVKTRIPG
                     VGTASAALFETRTNHEIVTLSNVGGRATYQNAGGTRRRGIELSADARLRDDWNARFAY
                     TLLDARYVDAFLTCTTTPCAAPTVPVEAGNRLPGVARSTLFASLGWTPPLGWRAGVEA
                     RLLSRVWVNDINSDAAAGYAVAAIDAGYRARFGPFSLDGFARVDNLLGRRYAGSVIVD
                     EGNSRYFEPAPGRTWTIGIAGSVRF"
BASE COUNT          369 a          860 c          983 g          379 t
ORIGIN      
        1 aagttgccga cggtgagggc acagcgcctg gcgaggtcgg ggtggcgaag gccgcgaaag
       61 acacgcatcg gccgattatg ttggcccgcg cctcgggcct cgtaggtccc ggccggcgag
      121 cgactgcgcc gagcgggcgt cgcggcgccg cagcctcaga accggacgga gccggcgatg
      181 ccgatcgtcc aggtgcggcc cggcgcgggc tcgaagtagc gcgaattgcc ctcgtcgacg
      241 atcaccgagc cggcgtagcg gcggccgagc aggttgtcga cgcgggcgaa cccgtcgagg
      301 ctgaacgggc cgaatcgtgc gcggtatccg gcgtcgatcg cggcgaccgc atagccggcg
      361 gccgcgtcgg aattgatgtc gttgacccag acccggctca gaaggcgtgc ctcgacaccg
      421 gcccgccagc cgagcggtgg cgtccagccg agcgaagcga agagcgtgga gcgggcgacg
      481 ccgggcagcc ggttgccggc ctcgaccggc accgtgggcg cggcgcacgg cgtcgtggtg
      541 caggtcagga acgcgtcgac gtagcgtgca tcgagcagcg tgtaggcgaa gcgggcattc
      601 cagtcgtcgc gaagccgggc atcggccgag agctcgatgc cgcggcgccg cgtgccgccg
      661 gcgttctggt aggtggcgcg gccgccgacg ttgctgagcg tgacgatctc gtggttcgtc
      721 cgcgtctcga acagcgcggc gctcgccgtg ccgacgccgg gaatgcgggt cttcacgccg
      781 gcctcgacgc tgtcgctcgt cgccgcctgc aggccgaggt cgaggccggt ggcgccgttc
      841 ggccggtacg ccagctcgtt gagggtcggc gtctcgaagc cgcggccggc ggtggcgtag
      901 agatgcacgt cgtcgctggc cgcgaagaga acgccgacga cggggagcgt cgcggcgtag
      961 ccgacgctgc cgctgtcgtc gggattggcg ccgacgatgt agtggtcgac cgagccgaaa
     1021 cggacgtggc tgcggcgcac gccggcatcg atcgtccagg ccggcgaggg cttgagcgtc
     1081 gcctgcacgt aggggtcgag gtcgacgacg cgattggtct cgtttcggcg cagcgctccc
     1141 tgcacgccga gcgtgggtcc gatgaagttc tggaagccct ggcgcgcctc gcgaagcgta
     1201 tcccaggcca gcccgccgac gacgccgagc ggcgcgtctc ccaggcgcgt ccgcaggctc
     1261 cagcgaacgt cgatgccgcg atagtcgcgg ccgagcgcga tcacgccgcc ggggttcagc
     1321 gggttcgtct gcgtcgccac cgggatcgac tggaattgct gggtgttgcg atggccgtcg
     1381 tagacgagcg cgcgcaacga attggcagca tcgagacggc gctcgtagac gatgccgagc
     1441 tggctctggt cgacggtctt gcgcgtgtcg aaggcgagcg cggccggatc gacgccgcgc
     1501 gggtcggctt cgaactgcgc ccgggtcagg ccgagcggat cgtcggcatt gggcagcgac
     1561 aggctgttgg cgaccagggt cagcttgctg tcgtcgtcgg gccgccacgt gagcttcatg
     1621 ttgccgatgc gtcgttcggc cgcgctgtgc tggcgatagc cgtcggtcga gaagtcgctg
     1681 ccgctgacga cgtagccgaa cgcgccgctc gctccgcgcg ccttgatcga ttcccgccgc
     1741 atgccgtcgc tgccggcgac caggctcgac gacagcgtcg gcgggccgct gccttcctcg
     1801 gtgaagactt gcacgacgcc gcccgaggag ttgccgtaca gcgccgagaa cggcccgcgc
     1861 aagacctcga tccggtcggc cgaaccgagg tcgacgttgg agatctggcc ctggccatcg
     1921 ggaagcgtcg ccggaatgcc gtcgacgtaa aggcggacac cgcgaatgcc gaacgtcgag
     1981 cgcgcgccga agccgcgcac cgacagctgc acgtcctgcg catagttctg ccggtcgcgg
     2041 gcaaccaggc cggcgaccgc gccgaggctt tccgagatgt tgatcatcgg ccgggcatcc
     2101 tcgatcgtct gccggtcgat ccggtcgatc gatgccggga cgtcgaaggc ggccgactcg
     2161 gtgcgcgtcg cggtgacgac caccggcgcc agctgggtga cgtcgccggc gacgggggcg
     2221 agggatttcg acgccggctg aggtttggcc tgcagttgcc gctccgcttg cgccggcact
     2281 ggcaccggcg tttgcgtttg cgtttgcgaa ggaacttgcg aaggcgtttg cgaaggcgct
     2341 tgcgacggca cctgcaatgc cggctgcgtc tgggcctgga caccggccgc cgccatcgcg
     2401 gccgcgacga acaggcatcg aagcgaggcc cgccgcgcgc tcgcatcggc gaatcgcccg
     2461 accgcgcgga cacaatccgg tcgccggcgg ctcgcgaagg cgaccttcat gcggtcgtcg
     2521 ctggcgcaac gggatcgtcg agcacgctgg ccgggccgtc gagccagcgc cattgtcccg
     2581 gggccagatc g
//
LOCUS       DASRWS010000158         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2356411, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000158 DASRWS010000000
VERSION     DASRWS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356411"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFF72_02870"
     CDS             <1..790
                     /locus_tag="VFF72_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZV92128.1"
                     /translation="RMHLLCDDESVIGRAFYVMEFVEGRVLWNQALPAFDPDGRAAIY
                     DEMNRVIAALHSVDPGAVGLAAYGKPGNYFERQIGRWTRQYQASITEPIEAMDRLIEW
                     LPSHIPPSARDDTQTSVVHGDFRLDNLVFHAEAPRVVAVLDWELSTLGHPLADFSYHC
                     MSWHIPPGSFRGIGGLDHAALGIPDEKSYVRRYCERTGRPDPDAVMADWNFYLAYNLF
                     RIAGILQGIAKRVEAGTASSAQARQSAAGARPLAEMGWQIARQA"
     gene            836..2077
                     /locus_tag="VFF72_02875"
     CDS             836..2077
                     /locus_tag="VFF72_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV92129.1"
                     /translation="MDFDYSPRTRELQAQLLRFMDEHIYPAEQRYEEEIEANTKAGKR
                     WTPLETIESLKPKARASGLWNLFLPPTAGGKGSKESGRTFGLSNSDYAPLAEIMGRVP
                     WSSEVFNCSAPDTGNMETIERYGSAEIKRQWLDPLLDGKIRSAFAMTEPNVASSDATN
                     IAARIERQGDEYVINGRKWWTSGAGDPRCKVFIFMGKTDPDAPRHSQQSMVVVPAETA
                     GITILRPLSVFGYDDAPHGHMEVDFKNVRVPVGNILLGEGRGFEIAQGRLGPGRIHHC
                     MRLVGLAERALELMCRRASSRVAFGRTVAEQGVTRERIADARCQIDMARLLTLKAAWM
                     MDVAGNKAAKAEIAMIKVVAPSMACQVIDWAMQAHGGGGMCQDFPLANFYAGARTLRF
                     ADGPDEVHRNAIAKIELGKHA"
BASE COUNT          362 a          744 c          696 g          317 t
ORIGIN      
        1 acggatgcat ctgctctgcg acgacgagtc ggtcatcggt cgcgccttct acgtgatgga
       61 gttcgtcgag ggtcgcgtgc tctggaacca ggcgttgccg gccttcgatc cggacggccg
      121 ggcggcgatc tacgacgaga tgaatcgcgt catcgccgcg ctgcattcgg tcgacccggg
      181 cgccgtcggc ctagcggcct acggcaagcc gggcaactac ttcgaacggc agatcggccg
      241 ctggacccgg cagtaccagg cgtcgatcac cgagccgatc gaggcgatgg accgcctgat
      301 cgaatggctg ccctcgcaca ttcccccgag cgcccgtgac gatacgcaga caagcgtcgt
      361 gcacggagac ttccgcctcg acaacctcgt cttccatgcc gaggcgccgc gcgtcgtcgc
      421 cgtgctcgac tgggagctgt cgaccctcgg ccatccgctc gccgacttca gctaccactg
      481 catgtcgtgg catatcccgc ccgggtcgtt tcgcggcatc ggcggcctcg accacgcggc
      541 cctcggcatt cccgacgaga agagctacgt gcgccgctat tgcgagcgca ccggccgacc
      601 cgaccccgat gcggtgatgg ccgactggaa tttctatctc gcctacaacc tctttcgcat
      661 cgccggcatc ctccagggaa tcgccaagcg cgtcgaggcc ggcacggcat cgagcgcaca
      721 ggcgcgccag tcggcggcag gcgcgcgacc gctcgccgag atgggctggc agatcgcccg
      781 gcaggcctga gggcgccggc cttcctcatt ccaggacacc accaaggacc acgccatgga
      841 cttcgactat tcgccgcgca ccagggaact gcaggcacag ctcctgcgct tcatggacga
      901 gcacatctat ccggccgagc agcgctacga agaggagatc gaggccaaca cgaaggccgg
      961 caagcgctgg acgccgctcg agaccatcga gtcgctcaag cccaaggcgc gggcgtcggg
     1021 cctgtggaat cttttcctgc cgcccaccgc cggaggcaag ggctcgaagg aaagcggccg
     1081 cacgttcggc ctgtcgaact ccgactacgc gccgctggcc gagatcatgg gccgcgtgcc
     1141 gtggtcgagc gaggtcttca attgctcggc gcccgatacc ggcaacatgg aaacgatcga
     1201 gcgctacggc tcggccgaga tcaagcggca gtggctcgac ccgctgctcg acggcaagat
     1261 ccgcagcgct ttcgcgatga ccgagcccaa cgtcgcctcg tccgacgcca ccaacatcgc
     1321 tgcgcgcatc gagcggcaag gcgacgaata cgtcatcaac ggccgcaagt ggtggaccag
     1381 cggcgccggc gatcctcgct gcaaggtctt catcttcatg ggcaagaccg accccgacgc
     1441 gccgcgtcat tcgcagcagt cgatggtcgt ggtgccggcc gaaacggcgg gcatcacgat
     1501 cctgcgcccg ctctccgtct tcggctacga cgacgcgccg cacggtcaca tggaggtcga
     1561 cttcaagaac gtccgcgttc cggtcggcaa catcctgctc ggcgaagggc gcggcttcga
     1621 gatcgcccag ggtcgcctcg gcccggggcg gatccaccac tgcatgcgcc tggtggggct
     1681 ggccgagcgg gccctcgagc tgatgtgcag gcgggcatcg agccgcgtcg ccttcggccg
     1741 caccgtcgcc gagcagggcg tgacgcgcga gcgcatcgcc gatgcccgtt gccagatcga
     1801 catggcgcgg ctgctgacgc tgaaagcggc gtggatgatg gacgtcgccg gcaacaaggc
     1861 cgccaaggcc gagatcgcga tgatcaaggt ggtcgcgccg tcgatggcct gccaggtgat
     1921 agactgggcg atgcaggcgc acggcggcgg cggcatgtgc caggacttcc cgctcgccaa
     1981 cttctatgcc ggcgcgcgca ccctgcgctt cgccgacggt cccgacgagg tgcaccgcaa
     2041 cgcgatcgcg aagatcgagt tgggcaagca cgcctgagcg aacgcgggct ccggcccgcg
     2101 ccgacctgcc gacccttca
//
LOCUS       DASRWS010000159         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1502951, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000159 DASRWS010000000
VERSION     DASRWS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502951"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFF72_02880"
     CDS             <1..345
                     /locus_tag="VFF72_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HZV92130.1"
                     /translation="YFGARRPDDGRIDWSRPAGEVYNLIRAVAPPYPGAFCEVAGRRL
                     VIAKARLASPLDASGPRPAAGLHVAGDRILGICGDGAAVIALEIRDEHDRAPLSLEAL
                     RSLLAADRAASP"
     gene            342..1394
                     /locus_tag="VFF72_02885"
     CDS             342..1394
                     /locus_tag="VFF72_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HZV92131.1"
                     /translation="MKKILILGVNGFIGHHLTQRILETTDWEVFGMDMAADRLGDDTS
                     HPRMHFFEGDITINKEWIEYHVKKCDVVLPLVAIATPATYVREPLRVFELDFEANLPI
                     VRAAVRHRKRLVFPSTSEVYGMCPDAQFDPDTSTLVYGPISKPRWIYACSKQLMDRVI
                     AAYGMEQGLDYTLFRPFNWIGPGLDSIFTKKEGSSRVVTQFLGQIVRGEPIQLVDGGR
                     QKRAFADIADGISALMTILRNPGGIASGRIYNIGNPSNHYSVRELAETMLAMAAQYPE
                     YAAAAADCRIADTSADDFYGEGYQDIDNRLPKIDATIRDLDWRPEVGMSESLRRIFES
                     YRSRVSEARSLTMLDA"
     gene            1400..>2157
                     /locus_tag="VFF72_02890"
     CDS             1400..>2157
                     /locus_tag="VFF72_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZV92132.1"
                     /translation="MARIALKIDVDTLRGTREGVPRLLELLAARNARATFLFSLGPDH
                     TGRALRRVFRPGFLAKVKRTSVLEHYGLRTLLYGTLLPGPDIGRRAAAEMKAVQAAGH
                     EVGIHTWDHVLWQDKVRDRDAAWTERQMQLAYDRFVDIFGERPATHGAAGWQTNDAAL
                     RRIDAWGMRYASDGRGGDPYRPLVGGRPLSHVQLPTTLPTLDELIGHDGIDKDNVADA
                     LLGRTEGAADQVFTLHAELEGGLFAPAFAALLRS"
BASE COUNT          348 a          777 c          696 g          336 t
ORIGIN      
        1 tatttcggcg cccgccgtcc cgacgacggc cgcatcgact ggtcgagacc ggccggcgag
       61 gtctacaacc tgatccgcgc cgtggcgccg ccgtacccgg gcgccttttg cgaggtcgcg
      121 gggcgacgcc tggtgatcgc gaaggcgcgc ctggcgagcc cgctcgatgc gagcggccca
      181 cgtcctgccg cgggcttgca cgtggccggc gaccggatcc tcggcatctg cggcgacggc
      241 gccgccgtca tcgccctcga aatccgcgac gagcacgacc gcgctccact gtcgctcgag
      301 gcccttcgtt ccctgctcgc cgccgatcgg gcggcctccc catgaaaaaa atcctgatcc
      361 tcggcgtcaa cggcttcatc ggccaccacc tcacgcaacg catcctcgag acgaccgact
      421 gggaagtctt cggcatggac atggcggccg accggctcgg cgacgacacg agccatccgc
      481 gcatgcactt cttcgagggc gacatcacga tcaacaagga gtggatcgag taccacgtca
      541 agaagtgcga cgtcgtcctg ccgctggtgg ccatcgccac gccggccacc tacgtgcgcg
      601 aaccgctgcg cgtcttcgag ctcgatttcg aggccaacct gccgatcgtg cgcgccgcgg
      661 tccggcacag gaagcgcctc gtctttccct cgacctccga ggtgtacggc atgtgccccg
      721 atgcgcaatt cgatcccgac acctcgaccc tcgtctacgg gccgatcagc aagccgcgct
      781 ggatctacgc ctgctcgaag cagctgatgg atcgcgtcat cgccgcgtac ggcatggagc
      841 agggcctcga ctacacgctg ttccggccgt tcaactggat cggccccggg ctcgattcga
      901 tcttcaccaa gaaggaaggt tcgtcgcggg tggtgacgca gttcctcggc cagatcgtgc
      961 gcggcgagcc gatccagctc gtcgacggcg gccggcagaa gcgggccttc gccgacatcg
     1021 ccgacggcat ctcggcgctg atgacgattc ttcgcaatcc cggcggcatc gcctcgggcc
     1081 gtatctacaa catcggcaat ccatccaacc attattcggt tcgcgagctg gccgagacga
     1141 tgctggcgat ggccgcgcag taccccgagt acgcggccgc ggcggcggat tgcaggatcg
     1201 ccgacacctc ggcagacgac ttctacggcg agggctatca ggacatcgac aaccggctgc
     1261 cgaagatcga cgccacgatc cgcgacctcg actggcggcc cgaggtcggg atgtcggaat
     1321 cgctgcggcg catcttcgag tcctatcgct cgagggtctc cgaggcacgg tcgctgacga
     1381 tgctcgacgc ctgacccgca tggcgcggat cgccctgaag atcgacgtcg acacgcttcg
     1441 cggcacgcgc gaaggcgtgc cgcggctgct cgagctcctg gcggcccgaa acgcgcgtgc
     1501 gacgtttctc ttcagcctcg gcccggacca caccggccgg gcgctgcggc gcgtctttcg
     1561 gcccggcttt ctcgccaagg tgaaacgaac ctcggtgctc gagcactacg gcctgcgcac
     1621 gctgctctac ggaacactcc tgccgggtcc ggacatcggc cgtcgcgccg cggccgagat
     1681 gaaggcggtg caagctgccg gccacgaggt cggcatccac acatgggatc acgtcctctg
     1741 gcaagacaag gtgcgtgacc gggacgccgc ctggaccgag cgccagatgc agctcgccta
     1801 cgaccgtttc gtcgacatct tcggcgaacg cccggccacc cacggtgcgg ccggctggca
     1861 gaccaacgac gcggcactgc ggcgaatcga tgcctggggc atgcgctacg cctccgacgg
     1921 gcgcggcggc gatccctacc ggccgctggt gggcggccgc ccgctgtcgc acgtccagtt
     1981 gccgaccacg ctgcccacgc tcgacgagct gatcggccac gacggcatcg acaaggacaa
     2041 cgtcgccgac gcgttgctgg gtcgaaccga aggcgcggcc gaccaggtct tcacgctgca
     2101 tgccgagctc gaaggcggcc tcttcgcgcc ggcgttcgcg gcgctgctgc gaagctg
//
LOCUS       DASRWS010000160         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_350873, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000160 DASRWS010000000
VERSION     DASRWS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350873"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1800
                     /locus_tag="VFF72_02895"
     CDS             1..1800
                     /locus_tag="VFF72_02895"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZV92133.1"
                     /translation="MDAMSLTLRRGETFALVGESGCGKSMTALALMRLLPENGRIVAG
                     RVLLEGEDMLALPESAMRSVRGGRIGMIFQEPGTSLNPVIRVGTQIVEAIEAHTALRG
                     AAARAKAIDWLGRVGIPEPERRIDEYPFRLSGGQKQRVMIAMTLASEPDFLIADEPTT
                     ALDVTIQAQILRLLKELQREQGMGLLLITHDLAVVSDMADRVALMYAGQIIEVADAAD
                     FFAAPRHPYARLLLQALPESARRGESLAAIRGSVPPLWLRFEGCRFAPRCDRAFAPCP
                     TTAPALTPLDGHSRSVRCLLYSEPGSHGAGVTASDGPASPRLPAAAAAGHDAGGAVDP
                     LLVVDNLAVRFPIRRGLLQRTAGYFSAVDGVSFEVAAGKTLALVGESGCGKTTTGKAI
                     VQLLRTTAVIEGGARFEGRDLFALEGEALRDARRRIQIIFQDPFSSLNPRMRVLAILD
                     EGMEALRPELDAGDRRARLEVLVEQVGLRRDALERFPHEFSGGQRQRIAIARALAVEP
                     RLIVCDEPTSALDVSVQAQILNLLSQLQRDLGVSLLFITHNIGVVEYLADDIVVMQRG
                     RVEESGSTRRVLDNPQSSYTRALLEAVPRMTVG"
     gene            2014..>2244
                     /locus_tag="VFF72_02900"
     CDS             2014..>2244
                     /locus_tag="VFF72_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92134.1"
                     /translation="MIAFGGAAIAPAYAQQTPPPAPPEQQLQRVEITGSSIKRIDAET
                     ALPVQVITREQIQHTGATNVEQLLETISAVSSS"
BASE COUNT          359 a          782 c          745 g          358 t
ORIGIN      
        1 atcgacgcca tgagcctgac cctgcggcgc ggcgagacct tcgcgctggt cggcgaatcg
       61 gggtgcggca agagcatgac cgcgctggcc ctgatgcgcc tgctccctga aaacggccgc
      121 atcgtcgccg ggcgggtcct cctcgagggc gaagacatgc tcgctctgcc cgagtcggcg
      181 atgcgatcgg tgcgcggtgg ccgcatcggc atgatcttcc aggaaccggg aaccagcctc
      241 aatccggtca tccgcgtcgg cacccagatc gtcgaggcga tcgaagcgca caccgcgctt
      301 cgcggcgccg cggcgcgggc caaggcgatc gactggctcg gccgggtcgg catccccgag
      361 ccggagaggc gcatcgacga gtatccgttc cggctgtcgg gcggacagaa gcagcgcgtc
      421 atgatcgcga tgacgctggc ctccgagccc gacttcctga tcgccgacga gccgacgacg
      481 gcgctcgacg tgacgatcca ggcgcagatc ctgcggctgc tgaaggagtt gcagcgcgag
      541 cagggcatgg gcctgttgct gatcacccac gacctcgccg tggtctccga catggccgac
      601 cgggtcgccc tgatgtacgc cggccagatc atcgaggtgg ccgacgcggc tgacttcttc
      661 gccgcgccgc ggcatcccta tgcccgtctg ctgttgcagg cactgcccga atcggcgcgc
      721 cgcggcgagt cgctggcggc gatccgcggt tcggtgccgc cgttgtggct cagattcgag
      781 ggatgccgct tcgcgccgcg ctgcgaccgc gcgttcgcgc cgtgcccgac gacggcacca
      841 gccttgacgc cgctcgacgg gcactcgcgc agcgtgcgct gcctgctcta cagcgagccc
      901 ggctcgcacg gcgccggggt gaccgccagc gatgggccgg cgtcgccgcg attgcccgcg
      961 gcggcggccg cggggcatga cgccggcggc gcggtcgatc ccttgctcgt cgtcgacaac
     1021 ctggccgtcc ggtttccgat tcgccgcggc ctgcttcagc gcaccgccgg atacttcagc
     1081 gccgtcgacg gcgtctcgtt cgaggtcgcg gccggcaaga cgctggcgct cgtcggcgaa
     1141 tcgggatgcg gcaagacgac gacgggcaag gcgatcgtgc aactgcttcg cacgaccgcc
     1201 gtgatcgagg gcggcgcgcg cttcgagggc cgggatctgt tcgcgctcga aggcgaggcg
     1261 ctgcgagacg cccggcgtcg catccagatc atcttccagg atccgttctc gtcgctgaac
     1321 ccgcgcatgc gcgtgctggc gatcctcgac gaaggcatgg aagccctgcg tcccgaactc
     1381 gacgctggcg accgccgtgc ccggctcgag gtgctcgtcg agcaggtcgg cctgcgccgc
     1441 gatgcgctgg aacgctttcc gcacgaattc tcgggcggcc aacggcaacg gatcgccatc
     1501 gcccgcgccc tcgcggtcga gccgcgactc atcgtctgcg acgagccgac ctcggcgctc
     1561 gacgtctcgg tccaggcgca gatcctgaac ctgctgtcgc agctgcagcg ggatctgggc
     1621 gtctcgctgc tcttcatcac gcacaacatc ggggtcgtcg aataccttgc cgacgacatc
     1681 gtcgtgatgc agcgcggacg cgtcgaagag agcgggtcga cacgccgggt gctggataac
     1741 ccgcagtcgt cgtacacccg cgccctgctc gaagccgtgc cgagaatgac tgttggttaa
     1801 agcagacgac tcgccaccaa ctgcagaaaa ttcgggtgaa cccgatcggg ccgttagttc
     1861 ccctccgtga caatagattt acagccttga cgctgccttg acactggttt acccgccggt
     1921 aacaagggca cagcaaggca accggtctat cgatccactt cttttcccgg agggaacatg
     1981 atgttgaaga gaacgaagct ctgcacgagc ctgatgatcg cctttggcgg cgcggcgatc
     2041 gccccggcct atgcccagca gacgccacct ccggctccgc ccgagcagca gctgcagcgc
     2101 gttgaaatca ccggctcgtc gatcaagcgg atcgacgcgg aaacggcgct tcccgtccag
     2161 gtcatcaccc gcgagcagat ccagcacacg ggcgcgacca acgtcgagca gctgctcgaa
     2221 acgatcagcg cagtctccag ttcc
//
LOCUS       DASRWS010000161         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1783788, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000161 DASRWS010000000
VERSION     DASRWS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783788"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFF72_02905"
     CDS             complement(<1..553)
                     /locus_tag="VFF72_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZV92135.1"
                     /translation="MSEISRVALFGKLNPVVYKTIEGATVFCKLRGNPYVELVHWLTQ
                     LLENSSSDIHAIVRHYALDAAALARDVTAALDRLPRGSTSLSDFAEHIPDAIERAWVY
                     GSLQFGDGQVRSGYLLLGILRTPSLRNALLAISRQFEKIKADDLADNFDAIVAKSGEN
                     GLGPQDGSGLSGGQPGEVSGAMAP"
     gene            complement(596..1702)
                     /gene="tssG"
                     /locus_tag="VFF72_02910"
     CDS             complement(596..1702)
                     /gene="tssG"
                     /locus_tag="VFF72_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HZV92136.1"
                     /translation="MRGSVADLGNVSVDPATGAAADEAFSVIEAHASRFDLFAAMRRI
                     ENACLDKPRLGDASRPSDETIRFAQDASLVFAPTAVVGLERREGRPPRLVQRVFGYMG
                     PNGALPIHLTEYARERETYNNDPTFARFLDTLLHRFGLFFYRAWARAQPVVGLDRPHD
                     APVVRHVGALIGIAERSLRDRDALGDAAKLFFAGRLARSVRDADGLEAWLSLRFEVPV
                     RVAQFQGHWMPLGRDERSRLQRQGQSALGRGAVLGRTVWDVQHKFRIVIGPLGWDRFS
                     SMLPGGRALEQLRALVRQYVGFEFAWDLRLILRRGDVPSWTLSGARDTRVGRLGRTAW
                     LNAGMRFRRDRDADDLIMNVESIRAAAVDSAVAV"
     gene            complement(1705..3576)
                     /gene="tssF"
                     /locus_tag="VFF72_02915"
     CDS             complement(1705..3576)
                     /gene="tssF"
                     /locus_tag="VFF72_02915"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HZV92137.1"
                     /translation="MDPRLLRYYNQELRYLREMGGEFARDFPKIAGRLGMEGMEVADP
                     YVERLIEGTAFLAARVQLKQDAEYTQLSQRLLEMICPNMTAPVPSMLVARIRPLPDPG
                     LIKGFRIERGSTLVGPETAMSRTRCEFRTAQEVVLTPLKMAAAEYFLSATDLPLSNLP
                     LPERPRSGVRIRLELPPGLSFGQLKLDALRLFIGGLGDVALRLHELITGACVGVLAGV
                     PARGNDAARQFLDGRCVRAVGLDDEDAMLPVTLRGLAGTRLMQEYFAFSQRFLFIDIG
                     SLQASFAKASGNAFEVVLLFDRYVAALEGAVESENFQLHCVPAINLFERRADRLHLDE
                     GATSYHLVPERTAALDYEVFDVLDVKAFRESSEEQAFLPLFATPQGERGAEGAYYSTV
                     REPRLPSDRGRREGPRSGYVGTETFLSLVDSHQSPYAGALRQLAVRVRCTNRDLPLFM
                     PTGQAGGELILSASAPIESISITAGPSRPQSAMREGAIAWRLLGLLSLNYLSLLDSDE
                     ETGAQALREILSLFAAAADVGLKRQIEGVRSVVVKPVVRRHPTPGPIAFSRGLEVKVT
                     VDDLSFEGGSAALLASVLHQYFSRHVSMNSFVQTVLASLTRGDVVTFTPRPGARTLL"
     gene            complement(3655..4188)
                     /gene="tssE"
                     /locus_tag="VFF72_02920"
     CDS             complement(3655..4188)
                     /gene="tssE"
                     /locus_tag="VFF72_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HZV92138.1"
                     /translation="MAGLTSQERLQPSLLDRLTDSEPASAREAIDARVLNKQQLRAAV
                     LRDLSWLFNTARSEPDPLRGAPEDVEMWRGAVEARNSVINYGIPSLSGVTISALQFEA
                     LELAIRQAIIQFEPRIDPKTLEVEVNRGAGFNIQHNSLRLIIRGHMWNQPVPLELLLS
                     ADVDVETGLAAVHDMRA"
BASE COUNT          758 a         1459 c         1470 g          640 t
ORIGIN      
        1 ccggtgccat cgcccccgac acttcgccgg gctggccacc cgagagcccc gagccgtcct
       61 gcggaccgag gccgttctcg ccgctcttgg cgacgatggc gtcgaaattg tcggcaaggt
      121 cgtcggcctt gatcttctcg aactggcgcg agatcgcgag caaggcgttg cgcaggctcg
      181 gtgtcctcag gatgcccagc agcaggtagc cgctgcgcac ttggccgtcg ccgaactgca
      241 gcgagccgta gacccacgcc cgctcgatcg catccgggat gtgctcggcg aaatccgaca
      301 gcgaggtcga gccgcgcggc aggcggtcga gcgccgcagt cacgtcgcgc gccagcgcgg
      361 cggcgtcgag cgcgtagtgg cgaacgatcg cgtggatgtc cgaactgctg ttctccagca
      421 gctgcgtgag ccagtgcacc agctccacgt agggattgcc gcggagcttg cagaagaccg
      481 tcgccccttc gatcgtcttg tagacgaccg gattgagctt gccgaacagt gccacccgac
      541 tgatttcact catggatcca ttcctcctct tgtcccttcg ccgtcgtcgc gtctcctaga
      601 cggccaccgc ggaatcgacc gccgccgcgc gaatgctctc gacgttcatg atcaggtcgt
      661 ccgcgtcgcg atcgcgcctg aaccgcattc cggcgttcag ccacgcggtg cgcccgagcc
      721 ggccgacgcg cgtgtcgcgt gcaccggaga gtgtccacga aggaacgtcg ccgcgcctca
      781 ggatcagccg caggtcccac gcaaattcga agccgacgta ctgccgaacg agcgcgcgaa
      841 gttgctcgag cgcgcggccg ccgggcagca tcgacgaaaa gcggtcccag ccgagcggac
      901 cgatgacgat gcgaaacttg tgctggacgt cccagacggt gcggcccagc actgcccccc
      961 ggccgagcgc ggattggccc tggcgctgaa ggcgcgaacg ctcgtcccgg ccgagcggca
     1021 tccagtggcc ctggaactgc gcgacacgca ccgggacttc gaaccggagc gacaaccaag
     1081 cctcgagccc gtcggcatcg cgcaccgatc gggccaggcg accggcgaag aagagcttcg
     1141 ccgcgtcgcc gagggcgtcg cgatcacgca ggcttcgctc ggcgatgccg atcagcgccc
     1201 cgacgtggcg aacgacgggc gcgtcgtgcg gccggtcgag cccgaccacg ggctgcgcgc
     1261 gcgcccatgc ccggtagaag aagaggccga agcgatgcag cagcgtgtcg aggaagcggg
     1321 cgaaggtcgg gtcgttgttg taggtctcgc gctcgcgcgc gtattcggtc aggtggatcg
     1381 gcaacgcccc gttcgggccc atgtagccga acacgcgctg caccaagcgc ggaggccgac
     1441 cctcgcggcg ctccaggccg acgaccgccg tcggcgcgaa gaccagcgac gcatcctggg
     1501 cgaagcggat cgtctcgtcg ctcggccgcg acgcatcgcc gaggcgaggc ttgtcgaggc
     1561 aggcgttctc gatgcgccgc atcgccgcga agagatcgaa gcgcgacgca tgcgcctcga
     1621 tgacggagaa cgcttcgtcg gccgcggcgc cggtggccgg gtcgacactg acgttgccga
     1681 gatcggcgac ggatccgcgc acgttcacag gagcgtcctt gcgcccggac gtggcgtgaa
     1741 ggtcaccaca tcgccccgtg tcagcgacgc gagcacggtc tggacgaagc tgttcatcga
     1801 gacgtgccgc gaaaaatact ggtgcagcac cgaggcgagg agcgccgcgc tgccgccctc
     1861 gaacgacagg tcatcgacgg tgaccttcac ctcgagcccc cgactgaagg cgatcggacc
     1921 cggcgtcgga tgtcggcgaa ccaccggctt cacgacgacc gagcgaacgc cttcgatctg
     1981 cctcttgaga ccgacatccg ccgcggcggc gaaaaggctc aggatctcgc gcaaggcctg
     2041 cgcaccggtc tcttcatcgc tgtcgagcag cgaaagatag ttgagcgaca acagtcccaa
     2101 caagcgccag gcgatcgcac cctcgcgcat cgcgctttgc ggccgtgacg gcccagcggt
     2161 gatcgaaatc gattcgatcg gcgcactggc gctcaggatg agctcgccgc cggcctgccc
     2221 cgtgggcatg aacaggggca gatcgcggtt ggtgcaacga acccgcacgg ccagctgtcg
     2281 caaggcgccg gcataaggtg actgatgcga gtcgaccagc gacaggaagg tttccgtgcc
     2341 cacgtagccc gacctcggtc cctcgcgcct gccgcggtcc gatggaagcc tcggctcgcg
     2401 caccgtgctg tagtaggcgc cctcggcgcc gcgctcgcct tgcggtgtcg cgaacaacgg
     2461 caggaaggcc tgctcctcgc tgctctcgcg aaaggccttg acatcgagaa cgtcgaacac
     2521 ctcgtaatcg agagcggcgg ttcgttcggg aaccaagtga tacgacgtgg cgccctcgtc
     2581 gagatggagg cggtcggccc gccgctcgaa gagattgatc gcgggaacgc aatgcagctg
     2641 gaagttctcg gactcgaccg caccctcgag cgccgcgacg tagcgatcga agaggaggac
     2701 gacctcgaag gcgttgccgg acgccttcgc gaacgaggcc tggaggctgc cgatgtcgat
     2761 gaaaaggaag cgctgcgaga aagcgaaata ctcctgcatc agccgcgttc cggcgaggcc
     2821 gcgcaaggtg accggcagca tcgcgtcttc atcgtcgagg ccgacagcgc ggacgcatcg
     2881 gccatcgaga aattgccgcg cggcgtcgtt gccgcgcgcc ggcacgcccg cgagcacgcc
     2941 gacgcaggca cccgtgatga gctcgtgcag gcgcagcgcg acgtcgccga ggccgccgat
     3001 gaacaggcga agcgcgtcga gcttgagctg gccgaaggaa aggcccggcg gaagctccag
     3061 ccggatccgc acgccgctgc gcggacgctc gggcagcggc aggttcgaca ggggcagatc
     3121 cgtcgcgctg aggaaatatt cggcggccgc catcttcagc ggcgtcagga cgacttcctg
     3181 cgcggtgcgg aactcgcagc gcgtgcggct catcgccgtc tccgggccaa cgagggtgct
     3241 tcctcgctcg atgcgaaagc ccttgatgag ccctggatcc ggaaggggcc tgatccgcgc
     3301 cacgagcatc gagggcaccg gcgccgtcat gttcgggcag atcatctcga gcaaccgctg
     3361 cgacaactgc gtatactcgg catcctgctt gagctgaacg cgcgcggcga ggaatgcggt
     3421 gccttcgatc agccgctcga cgtacggatc ggcgacttcc atgccttcca tgccgagccg
     3481 cccggcgatc ttcgggaagt cgcgcgcgaa ttcgccgccc atctcgcgca ggtagcgcaa
     3541 ctcctggttg taatagcgaa gaaggcgcgg gtccatgtca gagccgccgg cgcgatggtg
     3601 cgtccggcct cgcccgaggc aacagacgac ggtggtgcgt cgcgagcatc acggtcacgc
     3661 ccgcatgtcg tggacggcgg ccaggccggt ctcgacgtcg acatcggccg aaagaagcag
     3721 ctcgagggga accggctggt tccacatgtg gccacgaatg atgagccgca ggctgttgtg
     3781 ctggatgttg aagcccgcac ctcgattgac ctccacctcg agcgtcttgg gatcgatgcg
     3841 cggctcgaac tggatgatgg cctggcggat cgccagctcg agcgcctcga actgcaacgc
     3901 cgagatcgtg acgcccgaca gcgacgggat gccgtagttg atcaccgagt tgcgcgcctc
     3961 gaccgctcct cgccacatct cgacgtcttc cggggcgccg cgcaacggat cgggttcgga
     4021 ccgggcggtg ttgaacagcc aggaaaggtc gcgcaagacg gcggcgcgaa gttgctgctt
     4081 gttcaggacg cgagcgtcga tggcctcgcg tgccgaagcg ggttcggaat ccgtcagccg
     4141 gtcgagcaag gacggttgca gccgctcctg ggaagtcaga cccgccacta cgccgcctcg
     4201 ccggccgccg cgggtgcctc gtcgagctcg accgcacgca ccgacatgat ggcgtgatcg
     4261 cccgaatcgg tcgtccagat gcgctggccg aggccgtgcc agcggtcctc gccgacatcg
     4321 atccaat
//
LOCUS       DASRWS010000162         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_63620, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000162 DASRWS010000000
VERSION     DASRWS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63620"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFF72_02925"
     CDS             <1..254
                     /locus_tag="VFF72_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HZV92139.1"
                     /translation="DTPHLKTSVPIDSIGFADTVYEAGGLGRAMRLFRLPEANPHRHF
                     EFERSVDVAAAGDTPIYVRVTLENGHQAWSSPIYLFRRP"
     gene            275..1258
                     /locus_tag="VFF72_02930"
     CDS             275..1258
                     /locus_tag="VFF72_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92140.1"
                     /translation="MRTVSSCVALLGLCAGLTAAAPAALAQAWPSQKITIVVPFSAGG
                     GHDAMARICAEGLATRLGQPVIVENRPGANGMVGADNVVRAAPDGYTVLFSSPAEIVI
                     APSAYKTMRYDPEKDLIPVTLAATTPLVLVANPSLGVHTLPELIALAKRKPGSLSFGT
                     PGNGSSQQLAGAWLDSLAGIDMQHVPYKGAGPATNDVLAGTIPLAIVGMAPVLPLIKA
                     GKLVAIAVMTPERVPFAPDVPTVAETPGMAGFTASHWMGIFVPAHTPQPIVDQLQAAF
                     AAVLKTPAVRDKLLGMGIVAVGDTPAEFKAFLEADRVRFAKMYKLTGLTPE"
     gene            1263..2060
                     /locus_tag="VFF72_02935"
     CDS             1263..2060
                     /locus_tag="VFF72_02935"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HZV92141.1"
                     /translation="MNSDIPFDFVPIDGRRSRTKPRTSGLSMVIDDGLPLAYAASVLQ
                     MAAPYIDLMKIKTGTARLYDRSQLEAKLAMYRTAAVRSFIGGQFHEYVFAKLGEAALP
                     RFYDEALAVGFETIEISDNTVPLDDVQRRAQIEGAVRAGLKVFGEVGSKDTLSNPALL
                     VRQAQICFDAGAELVLVEAAELVDDDGKVRTSTLDIIKRELPVERVMIELPGPWIAGV
                     RACDIEAMKKTVFAEFGPNVNLANIDVRTIFDTEAQRVGLGTAGPLP"
     gene            2146..>2650
                     /locus_tag="VFF72_02940"
     CDS             2146..>2650
                     /locus_tag="VFF72_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92142.1"
                     /translation="MTNLRLALRCGLAVAIAASAAVAVAAGADTFPTRPVRLLVPLAA
                     GSTADILSRTLGAELTRAFGQQVVVENKPGAGGTIATAELARAPADGYTIEFASQGTL
                     VFNQSLYSRPGYDSLKDIAPILLVGGVSNVMVVPESSPANSPADVIAMARASTHELTF
                     SSGGNGTS"
BASE COUNT          415 a          961 c          859 g          415 t
ORIGIN      
        1 tcgacacgcc gcacctcaag acctcggtgc cgatcgactc gatcggcttc gccgacacgg
       61 tctacgaggc cggcggcctc ggtcgtgcca tgcggctgtt ccgcctgccg gaggcgaatc
      121 cgcaccgcca cttcgaattc gaacggagcg tcgacgtcgc tgctgctggc gacacgccga
      181 tctacgtccg cgtcacgctc gagaacggcc accaggcgtg gtcgagtccg atctacctgt
      241 ttcgccggcc ctgacaccga gcgaggagca cccgatgcgc accgtctctt cttgcgtggc
      301 cctgctcgga ctctgcgccg ggctcactgc cgcggcgccg gcggcactcg cccaggcgtg
      361 gccgtcgcaa aagatcacga tcgtcgtgcc tttttcggcc ggcggcggcc acgacgcgat
      421 ggcgcgcatc tgcgccgagg gactggcgac gcgtctggga cagcccgtca ttgtcgagaa
      481 caggccgggc gccaacggca tggtcggcgc cgacaacgtg gtgcgcgccg cgcccgatgg
      541 ctataccgtc ctgttctcgt cgccagccga gatcgtgatc gcgccgagcg cttacaagac
      601 gatgcgctac gacccggaaa aggacctgat cccggtgact ttggcggcca cgacgcctct
      661 cgttctcgtc gccaacccgt cactcggcgt gcacacgttg cccgagctga tcgccctggc
      721 caaaaggaag cccggctcgc tcagtttcgg cacgcccggc aacggcagct cgcagcagct
      781 cgccggggcc tggctcgact ctcttgccgg catcgacatg cagcacgtgc cctacaaggg
      841 cgccggcccg gcgaccaacg acgttctcgc cggcaccatt ccgctcgcca tcgtcggcat
      901 ggcgccggtg ctgccgctca tcaaggccgg caagctcgtt gcgatcgcgg tcatgacacc
      961 cgagcgcgtg ccgttcgcac cggacgtgcc gacggtcgcc gaaacgcccg ggatggccgg
     1021 gttcacggcc tcgcactgga tgggaatctt cgtgcccgcg cacacgccgc agcccatcgt
     1081 cgaccagctg caggcggcgt tcgcggcggt gctgaagacg cccgccgtgc gcgacaagct
     1141 gctcggaatg gggatcgttg cggtcggcga cacgccggcc gagttcaagg ccttcctcga
     1201 ggccgaccgg gtccgcttcg cgaagatgta caagctgacc gggctgacgc cggagtgaac
     1261 cgatgaacag cgatattccg ttcgacttcg tgccgatcga cggccggcgc agccgcacca
     1321 agccgcgcac cagcggcttg agcatggtga tcgacgacgg cctgccgctc gcctacgccg
     1381 cgagcgtgct gcagatggcg gcgccctaca tcgacctgat gaagatcaag accggcacgg
     1441 cgcgtctcta cgatcgatcg cagctcgaag ccaagctggc gatgtatcgc accgccgccg
     1501 tgcggtcgtt catcggcggc cagttccacg agtacgtgtt cgccaagctc ggcgaagccg
     1561 cgctgccgcg cttctacgac gaggcgcttg cggtcggctt cgagacgatc gagatctccg
     1621 ataacacggt gccgctcgac gatgtccagc ggcgcgccca gatcgaaggc gcggtgcgcg
     1681 ccggcctcaa ggtcttcggc gaggtcggat cgaaggacac gctcagcaac ccggcgctgc
     1741 tggtgcgcca ggcgcagatc tgcttcgacg ccggggccga gctggtgctg gtcgaggcag
     1801 ccgagctggt cgacgacgac ggcaaggtcc gcacttcgac cctcgacatc atcaagcgcg
     1861 agttgccggt cgagcgcgtg atgatcgagc tgcccggacc gtggatcgcc ggcgttcgcg
     1921 cctgcgacat cgaggcgatg aagaaaaccg tcttcgccga gttcggaccc aacgtcaacc
     1981 tcgccaacat cgacgtcagg acgatcttcg ataccgaggc ccagcgcgtc ggcctgggca
     2041 cggcggggcc gctgccctag ggccaagggc cgggccgaaa ggtcgtcgcg ccgagcccgg
     2101 ggcgcattcc acggctttgc acgcatgtcc acttccgcat ccgccatgac aaaccttcgc
     2161 ctcgcgcttc gctgtggtct cgctgtcgcc attgccgcgt ctgccgcggt cgccgtggcg
     2221 gccggtgccg ataccttccc gacgcggccg gtccgcctgc tcgtgccgct ggccgccggc
     2281 agcaccgccg acatcctgtc gcgcactctc ggcgccgaac tgacccgggc gttcggccag
     2341 caggtcgtcg tcgagaacaa gcccggcgcc ggcggcacca tcgccaccgc agagctcgcg
     2401 cgcgcgccgg ccgatggcta cacgatcgag ttcgcctcgc agggcacgct cgtcttcaac
     2461 cagtcgctct actcgcggcc cggctacgac tcgctcaagg acatcgcgcc catcctgctc
     2521 gtcggcggcg tctccaacgt catggtggtg cccgaatcga gccccgcgaa ctcgccggcc
     2581 gatgtcatcg ccatggccag ggccagcact cacgagctga cgttctcgtc gggcggcaac
     2641 ggcaccagcc
//
LOCUS       DASRWS010000163         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1784216, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000163 DASRWS010000000
VERSION     DASRWS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784216"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..645)
                     /locus_tag="VFF72_02945"
     CDS             complement(112..645)
                     /locus_tag="VFF72_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZV92143.1"
                     /translation="MAYDERLLGGNEHKKAPYRALQPFGQVPAYEEDGLALFESGAIV
                     LHIAERSTALMPASDPGRAQTKSWMFAALSSVEPPILALRAVGDSPGPARDRAAALVD
                     ERLRSLAAALEGRPHLLGAFSAADLLMTTVLREVESLASHPALEAYRDRCMTRPAFRK
                     ALADQLRPFAANAPAPA"
     gene            complement(741..1217)
                     /locus_tag="VFF72_02950"
     CDS             complement(741..1217)
                     /locus_tag="VFF72_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZV92144.1"
                     /translation="MNTLVNPPGTPATALVLRRTLDASPETAFAAWADPDHVREWWHP
                     EGFTTPSFEMDFRVGGQYRYCIRKDGNDGWARGTYREIVAPRRIVFTFRWEKDDLSNE
                     GETLITVTFEPEGDRTRLTFRQEPFADGASRHNHGQGWKQVLDAFDAFVATTKARP"
     gene            complement(1260..1667)
                     /locus_tag="VFF72_02955"
     CDS             complement(1260..1667)
                     /locus_tag="VFF72_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZV92145.1"
                     /translation="MDSLTATFAALADPTRRAILARLAAGDAVVKDIASPFAMSSPAV
                     SKHLRVLERAGLVEQGRERQWRPRRLRAEPLAEAANWLEGYRPFWDEKLDRLEAFVRK
                     LETTRRAAVPTAPASKRAPSAPPPPRTRKPARR"
     gene            1844..>3057
                     /locus_tag="VFF72_02960"
     CDS             1844..>3057
                     /locus_tag="VFF72_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZV92146.1"
                     /translation="MSRVAIAVGAVVVTLALVVVALNLMSGEKKIERRLSRLYPIESP
                     QFAQELGVLLGPPVEGGNRYRVLRNGDEIFPPMLGAIRGARTSINFETYIYWSGDIGR
                     AFADALTERARAGVPVHVMLDWVGSIKMDKALVEELEAAGVQVRKYHPLSWWQIGRFN
                     NRTHRKLLIVDGRVGFTGGVGIAPTWTGHAQDPDHWRDTHFEIEGPVVAQMQAVFLDN
                     WIKVAGEVLHGPDYFPRIEPAGDGRAQMFSSSPTGGSESMELMYLLAITAASKSIDLS
                     AAYFVPDDLTRAALIAAMKRGVRVRVIVPGKHIDSETVRMASRDRWGPLLEAGAVIAE
                     YRPTMFHCKVMIVDGLFLSVGSTNFDNRSFRLNDEATLNIVDTAFAAEQTAIFEDDLA
                     HASVVSLAQWRN"
BASE COUNT          466 a         1007 c         1115 g          469 t
ORIGIN      
        1 gccaagctct acgagatcgg tgccggcacg agcgagatcc ggcgcatgct gatcggccgc
       61 gagctgtacg ccgagagcat gtgacggcgc gtcgcgtcgg cggggcgaat gtcaggcagg
      121 cgccggcgcg ttggcggcga acggcctgag ctggtcggcg agggccttcc ggaacgccgg
      181 ccgggtcatg catcgatcgc gatacgcctc gagcgccgga tgcgacgcca gcgactcgac
      241 ctcgcgcagc accgtcgtca tcagcaggtc ggccgcgctg aatgcgccga gcagatgcgg
      301 ccgtccttcg agcgccgcgg ccaggctgcg caggcgctca tcgacaagag cggcggctcg
      361 atcgcgtgcc ggcccgggcg agtcgccgac ggcgcgcaac gcaaggatcg gcggctcgac
      421 gctgctgagc gcggcgaaca tccagctctt cgtctgcgcg cggccggggt cgctggccgg
      481 catcagcgcc gtcgagcgct cggcgatgtg cagcacgatc gcgcccgatt cgaagagggc
      541 gaggccgtct tcctcgtacg ccggcacctg cccgaacggc tgcagggccc ggtacggcgc
      601 cttcttgtgc tcgttgcccc cgagcagccg ctcgtcgtag gcgatgcctg cctcctagag
      661 cgcccagcgc acgcgcaggt cgcgaacgaa gccctgggcg aacggtggca cccatttgaa
      721 ggcgtagacg accggggcgc tcatggccgg gccttcgtcg tggcgacgaa cgcatcgaag
      781 gcgtcgagca cctgcttcca gccctggccg tggttgtggc gcgatgcgcc gtcggcgaag
      841 ggttcctgac gaaaggtgag ccgggtcctg tcgccctcgg gttcgaaggt gacggtgatc
      901 agcgtctcgc cttcgttcga gaggtcgtcc ttctcccagc gaaacgtgaa gacgatgcgc
      961 cgcggcgcga cgatctcgcg atacgtgccg cgcgcccagc catcgttgcc gtccttgcgg
     1021 atgcagtacc ggtactggcc gccgacgcgg aaatccatct cgaacgaggg cgtcgtgaag
     1081 ccttcgggat gccaccattc gcgcacgtgg tccgggtcgg cccaggcggc gaaggcggtc
     1141 tcgggcgacg cgtcgagcgt gcggcgaagg accagcgcgg tcgccggcgt gcccggcggg
     1201 ttgacgaggg tgttcatgca gtgatcctct gtgtggtggt gaggtggcgg tcgtcgcgtt
     1261 caccggcgag ccggctttcg cgtccgcggc gggggcggcg cagacggggc gcgcttcgag
     1321 gccggcgcag tcgggacggc ggcgcggcgc gtggtttcga gcttgcggac aaaggcctcg
     1381 aggcgatcga gcttctcgtc ccagaaaggc cggtagccct cgagccagtt tgccgcctcg
     1441 gcgagcggct cggcgcgcag acggcgcggc cgccattgcc tttcccgacc ctgctcgacg
     1501 aggccggcgc gttcgagaac gcgcaggtgc ttcgatacgg caggcgagct catcgcgaac
     1561 ggcgaagcga tgtccttgac gaccgcatcg ccggcggcga gccgcgcaag gatggcgcgg
     1621 cgggtcgggt cggcgagtgc ggcgaaggtg gcggtcagtg aatccatgcg gtcatggtaa
     1681 ccaatcggtt aaatatccgc aaggttattc atcgcccgct tcgcagcccg ttcgcggcag
     1741 ctcacgccga cgttaagctc gtgcccatgt cgagcgcgtg cgagcaggag catcgaggcg
     1801 aggcccggtc gggtcgggcg gctggaattt ccacgggtgt cgcatgagcc gcgtcgccat
     1861 cgccgtgggc gcggtcgtcg tcacgctggc gctcgtggtc gtcgcgctca acctgatgag
     1921 cggcgagaag aagatcgagc ggcgcctgag ccgcctctac ccgatcgaga gcccgcagtt
     1981 cgcgcaggag ctgggcgtcc tgctcggacc gccggtcgag ggcggcaacc gctatcgcgt
     2041 gctgaggaac ggcgacgaga tcttcccgcc gatgctgggc gcgatccgcg gtgcgaggac
     2101 gtcgatcaac ttcgagacct acatctactg gtcgggcgac atcggccgcg cgttcgccga
     2161 cgcgctgacc gagcgggcga gggcgggcgt tcccgtgcac gtgatgctcg actgggtcgg
     2221 cagcatcaag atggacaagg ccctcgtcga agagctcgag gcggccggcg tgcaggtgcg
     2281 caagtaccac ccgctcagct ggtggcagat cggccggttc aacaaccgca cccaccgcaa
     2341 gctgctcatc gtcgacggcc gggtcggctt cacgggaggc gtcggcatcg cgccgacgtg
     2401 gacggggcac gcccaggatc ccgaccactg gcgcgatacc cacttcgaga tcgagggccc
     2461 ggtcgtcgcg cagatgcagg cggtcttcct cgacaactgg atcaaggtcg cgggcgaggt
     2521 cctgcatggc cccgactact ttccccgcat cgagccggcc ggcgacggcc gggcgcagat
     2581 gttcagcagc tcgcccaccg gcggcagcga gagcatggag ctgatgtacc tgctggcgat
     2641 cacggcggcg tcgaagagca tcgatctgtc ggcggcctac ttcgtccccg acgacctgac
     2701 gcgcgcggcg ctgatcgcgg cgatgaagcg cggggtgcgg gtgcgcgtga tcgttcccgg
     2761 caagcacatc gactcggaga cggtccggat ggcctcgcgc gaccgctggg ggccgctgct
     2821 cgaggccggc gccgtcatcg ccgaataccg gccgacgatg ttccactgca aggtgatgat
     2881 cgtcgacggc ctgttcctgt cggtcggctc gaccaacttc gacaaccggt cgtttcgcct
     2941 caacgacgag gcgacgctca acatcgtcga taccgccttc gcggccgagc agaccgcgat
     3001 cttcgaggac gacctcgcgc acgcgagcgt cgtgtcgctg gcgcagtggc gaaacag
//
LOCUS       DASRWS010000164         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_922431, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000164 DASRWS010000000
VERSION     DASRWS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922431"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1085
                     /locus_tag="VFF72_02965"
     CDS             102..1085
                     /locus_tag="VFF72_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZV92147.1"
                     /translation="MQYKDYYETLGVPKTATHDEIKQAYRKLARKYHPDVSKLADAET
                     RFKEINEANEVLKDPEKRAAYDQVGSGFKAGQEFQPPPNWDAGFEFRGGGGEAPGGFG
                     SFGGEGVDASDFFESLFGARARGGAARPRRNAAMQGEDHHAKVLVDLDDAYHGAERSI
                     SLRVPKEGPDGRVVLEERRLDVHIPKGIRPGQHLRLAGQGAPGSGGAPAGDLYLEIEF
                     KPHPAYRVDGADVYVDLPLAPWEAALGATVDAQTPDGTVQLSVPKGSAAGRKLRLKGR
                     GLPARGKGTPGDLYAVLAIALPAADTDKARDAYASMASTFAGFDPRRARGG"
     gene            1089..1403
                     /locus_tag="VFF72_02970"
     CDS             1089..1403
                     /locus_tag="VFF72_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZV92148.1"
                     /translation="MDESRHLALRGSVVEDELALTTFELCRACNASEEYIEMWVSEGV
                     LQPSGGSREQWRFTGQALTRMRTAMRLSRDLDLNAPGVALALDLLDEIEALEARLRRR
                     SG"
     gene            complement(1318..1860)
                     /locus_tag="VFF72_02975"
     CDS             complement(1318..1860)
                     /locus_tag="VFF72_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZV92149.1"
                     /translation="MIVLDRKDHAILDALQRDSRQTLQQLASAVGLTSTPCWKRVKAM
                     EAAGVIRGYGAVVDRHSVGLALCVLAEVNLTQHTESTVREFERAVAACPEIVACYSTT
                     GGADYSIKVLVPDIRTYEAFLHETAFRLPGVTHIRSSVVLKEVKAEAGVPLTRSDVAA
                     SPRAPRSRPASRAPAPRRAR"
     gene            1986..>2344
                     /locus_tag="VFF72_02980"
     CDS             1986..>2344
                     /locus_tag="VFF72_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92150.1"
                     /translation="MPDTLARPDYRLADNLAGEDGVVFLTGTQALIRLPLMQARLDAA
                     RGIDSQGFVSGYRGSPLGMVDQQAWKAKKLLDAAGVKFLPAINEELGATAVLGTQRVE
                     ADPERTCDGVFAMWYGKG"
BASE COUNT          387 a          807 c          826 g          324 t
ORIGIN      
        1 ggcctcgacc gaagccgtga tgccggcttg cggcggcccg atggcgcccc caactgaagc
       61 acgcgaagcg gcccggctgg ccccgacgca ctgaccacaa gatgcaatac aaggactact
      121 acgagaccct gggcgtgccg aagacggcga cgcacgacga gatcaagcag gcctaccgca
      181 agctcgcccg caagtaccac cccgacgtca gcaagctcgc cgacgccgag acgcgcttca
      241 aggagatcaa cgaggccaac gaggtcctca aggatcccga gaagcgcgcc gcgtacgacc
      301 aggtcggcag cggcttcaag gcgggccagg agttccagcc gccgccgaac tgggacgccg
      361 gcttcgagtt ccgtggcggc ggcggcgagg cgccgggcgg cttcggctcg ttcggcggcg
      421 agggggtcga cgccagcgac ttcttcgaat cgctcttcgg cgcgagggcg cgaggcggcg
      481 ctgcgcgtcc gcggcgcaac gccgcgatgc agggcgagga ccaccacgcc aaggtgctcg
      541 tcgatctcga cgatgcctat cacggcgccg agcgcagcat cagcctgcga gttcccaagg
      601 aaggtcccga cggccgggtc gtcctcgaag agcgccgcct cgacgtccat atcccgaagg
      661 gcatccgccc cggccagcac ctgcgcctgg ccggccaggg cgcgccaggc agcggcggcg
      721 cgcccgccgg cgatctctac ctcgagatcg aattcaagcc gcatccggcc tatcgcgtcg
      781 acggcgccga cgtctatgtc gacctgccgc tcgcgccatg ggaagcggcg ctcggcgcga
      841 ccgtcgatgc gcagacaccc gacggcacgg tgcagctcag cgttccgaag ggctcggctg
      901 ccggccgcaa gctgcgcctg aagggccgcg gcctgccggc gcgcggcaag gggacgccgg
      961 gcgatctcta cgcggtgctg gcgatcgcgc tgccggccgc ggataccgac aaggcacgcg
     1021 acgcgtacgc atcgatggcg agcacgttcg ccgggttcga cccgcgtcgt gcccgcggag
     1081 gctgaaccat ggatgaatcg agacacctcg cgctgcgcgg cagcgtcgtc gaagacgagc
     1141 tggccctcac gaccttcgag ctctgccggg cctgcaacgc cagcgaggag tacatcgaga
     1201 tgtgggtgag cgagggcgtg ctgcagccaa gcggcggctc gcgcgagcag tggcgcttca
     1261 ccggccaggc gctgacgcgc atgcgcacgg cgatgcggct gagccgcgac ctcgacctca
     1321 acgcgcccgg cgtggcgctg gcgctcgact tgctggacga gatcgaggcg ctcgaggcga
     1381 ggctgcgacg tcgctccggg tgagcggcac gccggcctcg gccttgacct ccttcagcac
     1441 gacgctcgag cggatgtggg tgacgccggg caggcgaaac gcggtctcgt gcaggaacgc
     1501 ctcgtaggtc cggatgtctg gcaccagcac cttgatcgaa tagtcggcgc cgccggtcgt
     1561 gctgtagcag gcgacgatct cgggacaggc ggcgacggcg cgctcgaatt cgcgcaccgt
     1621 gctttcggtg tgctgcgtca ggttgacctc ggcgagcacg cacagcgcca ggccgacgct
     1681 gtggcgatcg acgacggcgc cgtagccgcg gatgacgccg gccgcttcca tcgccttgac
     1741 gcgcttccag caaggcgtcg aagtcaggcc gacggcgctg gcgagctgct gcaacgtctg
     1801 gcgcgaatcg cgctgcaggg cgtcgaggat cgcgtggtcc ttccggtcga gaacgatcat
     1861 gctgtcgagt gccgaaaatc gccgcatccg ttccacggca ggtcgattct gcgacaaaga
     1921 aagaatccca ttcttcgctt gcgtcactaa attggggtca tccgcgtcgt ccgatcggaa
     1981 tcgccatgcc cgacaccctc gcccgtcccg actatcgcct cgccgacaac ctggccggcg
     2041 aggacggcgt cgtcttcctc accggcacgc aggcgctgat ccggctgccg ctgatgcagg
     2101 cgcggctcga tgccgcgcgc ggcatcgatt cgcaaggctt cgtcagcggc taccgcggct
     2161 cgccgctcgg catggtcgac cagcaggcgt ggaaggcgaa gaagctgctc gatgcggcgg
     2221 gcgtgaagtt cctgcccgcg atcaacgagg agctcggtgc gaccgcggtg ctcggcacgc
     2281 agcgcgtcga ggccgatccg gagcgcacct gcgacggcgt gttcgcgatg tggtacggaa
     2341 aggg
//
LOCUS       DASRWS010000165         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3807983, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000165 DASRWS010000000
VERSION     DASRWS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3807983"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1490)
                     /locus_tag="VFF72_02985"
     CDS             complement(294..1490)
                     /locus_tag="VFF72_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZV92151.1"
                     /translation="MKKKPPSSTSLIAHAYAPPPGFESVQPAVHKASTVVFRNAAALR
                     ARSWKEKTGYTYGLHGTPTTFTLEERIATLEGGTSTLLVPSGLAAITLVGAALLRAGD
                     EILIPDNVYGPSRELARNELAAWGIAHRSYDPMDLASLAAALSPATRLVWLEAPGSVT
                     MEFPDLPALVDEVRRRPGVTVAIDNTWGAGLAFDPFDLGGSRAVDVSVQALTKYPSGG
                     GDVLMGSVTTRDPSLHQRLKATHMRMGWGVGANDVEAVLRSLPSLAVRYAAQDRAGRE
                     LAGWWSKRPEVTAVLHPALAGSPGHEHWQALCSQAAGLFSVVFDARFATRQVDAFVDA
                     LKLFRIGYSWAGPASLVVPYDLAAMRPQASAQGGGVVRFSLGLEDVADLVADCEQGLK
                     ALDRPA"
     gene            complement(1499..>2236)
                     /locus_tag="VFF72_02990"
     CDS             complement(1499..>2236)
                     /locus_tag="VFF72_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZV92152.1"
                     /translation="GFWNGRPVKTQMLALCEQLAAVAAPHGAWVRLHYVYPYPHVDDV
                     LPLMATGAILPYLDVPFQHSHPDVLRRMKRPASGEKNLERLARWREICPELVVRSTFI
                     AGFPGETEAEFEDLLQFVREARIDRAGCFAYSAVAGATANELPGMLPIEVREERRARF
                     MAVAEEVSTGKLGLRVGATMQVLVDSAPALGRRGGFGRSYADAPEIDGRVRLLPPEKA
                     SKTLKTGEFTRARIVATEGHDLVGLPI"
BASE COUNT          352 a          794 c          783 g          307 t
ORIGIN      
        1 aatcctcgag atgaagatcg cgctggcgac cctgttgcgc catttcgatg tcgagagcgt
       61 cgacaccgcc gacggcggcg agcccgacga gatcatgtcg ttcacgatga cgccgagcac
      121 gctggccatg cgactgcgcg aacgagaggc cgcctccttc cgctgaagcg gccggcgtcg
      181 caccctcggg aggcgccggc acccggccgg cggtcaggcc gggcggtcga gcgccttcag
      241 cgcctgttcg cagtcggcga cgagatcggc gacgtcttcc agtccgcccg cagtcaggcc
      301 gggcgatcga gcgccttcag cccctgttcg cagtcggcga cgagatcggc gacgtcttcc
      361 agtccgagcg agaagcggac gacgccgccg ccctgagccg aggcctgcgg ccgcatcgcc
      421 gcgaggtcgt acggcacgac caggctggcc ggccccgccc acgagtagcc gatgcgaaag
      481 agcttcaggg catcgacgaa ggcatcgacc tggcgggtcg cgaagcgcgc gtcgaagacg
      541 accgagaaga gccccgccgc ctgcgagcac aacgcctgcc aatgctcgtg gccgggcgac
      601 ccggccagcg ccggatgcag gacggccgtg acctccggcc gcttcgacca ccagccggcg
      661 agctcgcggc cggcgcgatc ctgtgccgcg tagcgaaccg ccagcgatgg aagcgatcgc
      721 aacaccgcct cgacgtcgtt cgcgccgacg ccccagccca tgcgcatgtg cgtcgccttg
      781 agtcgctggt gcagcgacgg atcgcgcgtc gtcaccgaac ccatcagcac gtcgccgccg
      841 cccgaggggt acttcgtcag ggcctggacc gacacgtcga ccgcgcgcga acctcccagg
      901 tcgaacgggt cgaaggcgag ccccgcgccc caggtgttgt cgatcgcgac ggtgaccccg
      961 ggacggcgac gcacttcatc gaccagcgcc ggcaggtccg gaaactccat cgtcaccgat
     1021 cccggcgctt caagccagac gagccgcgtc gccggcgaca gcgccgcggc gagcgaggcg
     1081 aggtccatcg gatcgtagga acggtgagcg atgccccagg cggcgagctc gttgcgcgcc
     1141 agctccctgc tcgggccgta gacgttgtcc ggaatcaaga tctcgtcacc ggcgcgaagc
     1201 agcgccgcgc cgacgagcgt gatcgccgcc aggccgctcg gcacgagcag cgtcgacgtg
     1261 ccgccctcga gcgtcgcgat ccgctcctcg agcgtgaagg tcgtcggcgt gccgtgcagg
     1321 ccgtaggtgt agccggtctt ttccttccag ctgcgcgccc gcagcgccgc ggcgtttcga
     1381 aagacgacgg tcgacgcctt gtgcacggcc ggctgcaccg actcaaagcc cggcggcggc
     1441 gcgtaggcgt gggcgatgag cgaggtgctg gagggcggct tcttcttcat ggcgtgtgtc
     1501 agatcggcag gccgacgagg tcgtggccct cggtcgcgac gatgcgcgcg cgcgtgaact
     1561 cgcccgtctt cagtgtcttc gacgccttct cgggcggcag caaccgcacg cggccgtcga
     1621 tctcgggcgc gtcggcgtag ctgcgaccga aaccgccccg ccggccgagg gcaggcgccg
     1681 agtcgacgag cacctgcatc gtcgcgccga cgcgcaggcc gagcttgccg gtcgacacct
     1741 cttcggcgac ggccatgaag cgggcgcgtc tttcctcgcg cacttcgatg ggcagcatgc
     1801 cgggcagttc gttggcggtc gcgcccgcga ccgccgaata ggcgaagcag ccggcccgat
     1861 cgatgcgtgc ctcgcgaacg aattgcagga gatcttcgaa ctcggcctcg gtctcgcccg
     1921 gaaacccggc gatgaaggtg ctgcgaacga ccagctcggg acagatctcg cgccagcgcg
     1981 cgagccgctc gaggttcttc tcgccgctgg ccgggcgctt catgcggcgc agcacgtcgg
     2041 gatgcgaatg ctggaacggc acgtcgaggt agggcaggat cgctccggtc gccatcagcg
     2101 gcagcacgtc gtcgacgtgc gggtacggat agacatagtg cagccgcacc caggcgccat
     2161 gcggcgccgc gaccgcggcg agctgctcgc aaagcgcgag catctgcgtc ttcacggggc
     2221 ggccgttcca gaagcc
//
LOCUS       DASRWS010000166         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1509310, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000166 DASRWS010000000
VERSION     DASRWS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509310"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /gene="pgl"
                     /locus_tag="VFF72_02995"
     CDS             <1..550
                     /gene="pgl"
                     /locus_tag="VFF72_02995"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZV92153.1"
                     /translation="AAYELLRHADADWASWHVFFGDERCEPPDDPQRNSRMARTAWLA
                     HVATPAGQVHEIEGERGAEEAARRAAATLSGVPDFDLVLLGLGEDGHTASLFPGHDAG
                     TSGDAPDALAVFNAPKPPPERVSLSAHRLGRAREVVFLVAGEEKREAVGRWRRGDDIP
                     ARAIAPPAGVDVLIEARLLSPA"
     gene            complement(701..1180)
                     /locus_tag="VFF72_03000"
     CDS             complement(701..1180)
                     /locus_tag="VFF72_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZV92154.1"
                     /translation="MSYIDSNLIAGERVVYRTRLHWSVFVVPVLFIVVILLPLAAWLV
                     ASNRQQLLWMPVVLAVLVLVPPVIKRQSSDFAVTNKRVMMKVGVFTTRSIELLLGKIE
                     AISVDQTVGGRIFGYGNIVVTGSGGTREAFSQIQGPLEFRKAVQSVSDGSPTPTAAA"
     gene            1371..>3292
                     /locus_tag="VFF72_03005"
     CDS             1371..>3292
                     /locus_tag="VFF72_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZV92155.1"
                     /translation="MASFLRPHPPLTTGLELASPLRARVTAACRRPEAVCVAEVLRDA
                     RLDPAAAGQAQALARRIALQLRGRKPSSGRPGIVQGLLQEYALSSQEGVALMCLAEAL
                     LRIPDAATRDALIRDKVARGEWQSHAGRSPSLFVNAATWGLLLTGKLVATHSEGHLLA
                     NLTRLVGKGGEPLIRKGVDLAMRMMGEQFVTGETIDRALDRARTLESQGFRYSYDMLG
                     EAAVTADDAARYRLAYEDAIRAIGRASAGRGIYDGPGISIKLSALHPRYCRAQRERVL
                     AELYPVLVELVTLARGVDVGINIDAEEADRLELSLDLLERLCAEPALAGWNGIGFVVQ
                     AYQKRCPAVIDLLVDLARRHGRRLMVRLVKGAYWDSEIKRAQVDGMDDYPVYTRKAHT
                     DVSYLACARKLLAAPEHIYPQFATHNAQTLAAIYWMADPARYRPGQYEFQCLHGMGEP
                     LYEQVVGRAEDGRLGRPCRIYAPVGTHETLLAYLVRRLLENGANSSFVNRIADPAIAL
                     ETLIEDPVTTLENTAAREGALGLPHPAIALPQGIYGAARANSRGLDLNDDDRLRALQA
                     TLAESAAEVWRAAPLLAESAATKAEAPETPASPWLDVRNPADHRDVVGQLREATPADI
                     EHALAWARRDATRWAQV"
BASE COUNT          487 a         1188 c         1145 g          472 t
ORIGIN      
        1 tgccgcctac gagctcctgc gccatgccga tgccgactgg gcatcgtggc acgtcttctt
       61 cggcgacgaa cgctgcgagc cgcccgacga cccgcagcgc aactcgcgga tggcccggac
      121 ggcatggctg gcccacgtgg ccactccggc cggccaggtg cacgagatcg aaggcgagcg
      181 gggtgccgaa gaagccgcgc gccgcgcggc ggcgacgctt tcgggcgttc ccgatttcga
      241 cctcgtgctg ctcggtctcg gcgaggacgg gcacaccgcc agcctctttc ccggccacga
      301 cgccggcacg tcgggcgatg cacccgatgc gctcgccgtg ttcaatgcac ccaagccgcc
      361 acccgagcgc gtgagcctga gcgcgcaccg actcggacgg gcgcgcgagg tcgtgttcct
      421 cgtcgccggc gaggaaaagc gcgaggccgt cgggcgctgg cgacgcggcg acgacatccc
      481 ggcccgcgcc atcgcgccgc cagccggggt cgatgtgctc atcgaggcgc ggctgctctc
      541 accggcctga tccggccact ccttcccgcc ggctcggtct gtcgcccgct cttgccggcc
      601 ggctcggctg ccgcgctcgc cgggctgacc ggtgtttcga ggacgcagcg ccgatgcggc
      661 ggcgcgaagc tcgccgagcg gcgggaatgc cgggaccgga tcaggccgcg gccgtcggcg
      721 tcggcgaacc gtcgctgacc gactgcacgg ccttgcggaa ctcgaggggg ccctggatct
      781 gcgagaacgc ctcccgcgtt ccgccgctgc cggtgacgac gatgttgccg tagccgaaga
      841 tcctgccgcc gacggtctgg tcgaccgaga tggcctcgat cttgccgagc agcagctcga
      901 tcgaccgcgt cgtgaagacg ccgaccttca tcatcacccg cttgttggtg acggcgaaat
      961 cggagctctg ccgcttgatg accggcggga cgaggaccag cacagcgagc accacgggca
     1021 tccagagcaa ctgctggcgg ttgctcgcga cgagccatgc ggccagcggc aggaggatga
     1081 cgacgatgaa gaggaccggc accacgaaca ccgaccagtg cagccgggtc cggtagacga
     1141 cgcgctctcc ggcgatcagg ttcgaatcga tgtagctcat cggctccagt atcgcgcact
     1201 gcgcggggtg ggcgagccgg gcgtccgttc ggcattcgct cggcggctgc gacccgttcg
     1261 tcgtctgccg gcgcggacgg cgacggcgac gacgacctcg gcacgcctcc cgcataccat
     1321 tcctctcgcc aaccccttcc gccagactcg ccgcgagcgc ctgcaccacc atggcttcgt
     1381 tccttcggcc gcacccgccg ctgaccactg gcctcgagct cgcgagtccc ctgcgcgccc
     1441 gcgtgacggc ggcctgtcgg cgacccgagg cggtatgcgt cgcagaggtg ttgcgcgacg
     1501 cccggctcga ccccgcagcc gccgggcagg cgcaggcgct ggcgcgacgc atcgccttgc
     1561 agcttcgcgg gcgcaagcca tcgtcggggc ggcccgggat cgtccagggc ctgcttcagg
     1621 aatatgcgct gtcgtcgcag gagggcgtgg cgctgatgtg cctggccgag gcgctgctcc
     1681 gcattccgga tgccgcgacg cgcgacgcgc tgattcgcga caaggtggcg cgcggcgagt
     1741 ggcaatcgca cgccggtcgc agcccgtcgc tgttcgtcaa cgcggcgact tgggggctgc
     1801 tgctgaccgg caagctcgtc gcgacgcaca gtgaaggcca tctcctggcg aacctcacgc
     1861 gcctcgtcgg caagggcggc gagccgctga tccgaaaggg cgtcgacctg gcgatgcgga
     1921 tgatgggcga gcagttcgtc accggcgaga cgatcgaccg cgcgctcgac cgcgcgcgca
     1981 cgctcgagtc gcagggcttt cgctactcgt acgacatgct cggcgaggcg gccgtcacgg
     2041 cggacgacgc cgcgcgctac cggctcgcct acgaagacgc gatccgggcg atcggccgcg
     2101 cctcggcggg ccgcggcatc tacgacggcc ccggcatctc gatcaagctt tcggcgctgc
     2161 atccgcgcta ctgccgggcg cagcgcgaac gcgttctggc cgagctctat ccggtcctcg
     2221 tcgagctggt gacgctggcc cgcggcgtcg acgtcggcat caacatcgac gccgaggaag
     2281 ccgatcgcct cgagctgtcg ctcgacctgc ttgaaaggct ctgcgccgag ccggccctcg
     2341 ccggctggaa cggaatcggc ttcgtggtcc aggcctacca gaagcgatgt cccgcggtga
     2401 tcgacctgct cgtcgatctc gcccgccggc acggtcgacg tctgatggtc cgcctcgtca
     2461 agggcgccta ttgggacagc gagatcaagc gcgcccaggt cgacggcatg gacgactacc
     2521 cggtctacac gcgcaaggcc cacaccgacg tgtcgtatct tgcctgcgcg agaaagctgc
     2581 tcgccgcccc cgagcacatc tatccgcagt tcgcgaccca caacgcgcag acgctggcag
     2641 cgatctactg gatggccgac ccggcacgct atcggccggg gcagtacgaa ttccagtgcc
     2701 tgcacggcat gggcgagccg ctgtacgagc aggtggtcgg ccgtgccgaa gacggccggc
     2761 tcggcaggcc gtgtcgcatc tacgcgccgg tcggcacgca cgagacgctc ctcgcctacc
     2821 tcgttcgccg gctgctcgag aacggcgcca acagctcgtt cgtcaatcgc atcgccgatc
     2881 cggccattgc gctcgagacg ctgatcgagg atccggtgac gacgctcgag aacacggcgg
     2941 cgcgcgaagg cgcattgggg ttgccgcatc cggcgatcgc cctgccgcag ggcatctatg
     3001 gcgcggcacg ggccaattcg cgcggcctcg acctgaacga cgacgaccgg ctccgcgcgc
     3061 tgcaggcgac gctcgccgag tcggcggccg aggtctggcg ggccgcaccg ctgcttgcgg
     3121 aatcggctgc gacaaaggcg gaggcgccgg agacgccggc gtcgccatgg ctcgacgtcc
     3181 gcaaccccgc cgatcaccgc gacgtcgtcg gccagctgcg cgaagcgacg ccggccgaca
     3241 tcgagcatgc actcgcctgg gcacggcggg atgcgacgcg ctgggcgcag gt
//
LOCUS       DASRWS010000167         4680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3241335, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000167 DASRWS010000000
VERSION     DASRWS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241335"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1405
                     /locus_tag="VFF72_03010"
     CDS             215..1405
                     /locus_tag="VFF72_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HZV92156.1"
                     /translation="MQVIESILADAAQIAAVRRDIHAHPELCFKEERTSDLIARQLTE
                     WGIPIQRGLGTTGIVAILKNGSSGRAVGLRADIDALPITEHNQFAHKSTHAGKMHACG
                     HDGHTAMLLAAAKHLSKHRNYDGTVYLIFQPAEEGGGGAREMMKDGLFEKFPMEAVFG
                     AHNWPGMKAGQFALKSGPAFASSNEFHITVRGKGSHAAMPHLGVDPVPVACQIVNAFQ
                     TIISRNKRPIDAGVISVTMIHAGEATNVVPDSCTIEGTVRTFTLEVLDLIEKRMKEVV
                     EHTCAAFDATCEFAFKRNYPPTINHPAETEFARKVMQDMVGAENVLEFEPTMGAEDFS
                     YFLLEKPGCYFLIGNGDGSHREGGHGMGPCMLHNPSYDFNDDLIPLGGSLWVRLAEAW
                     LAPR"
     gene            1402..2493
                     /locus_tag="VFF72_03015"
     CDS             1402..2493
                     /locus_tag="VFF72_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HZV92157.1"
                     /translation="MSAAAGFAQTYAEAREKFLAAARNRGLGIETHVHPLPGRDGEML
                     AMDVARDGAADAASVLIVSSACHGVEGFCGSGVQVALLGDDAWHRDCADAGVAVLYVH
                     GLNPYGFSWWRRTTHENVDLNRNFRDFAQPLPRNEGYDDLAELIVPEVWPPDEATTRG
                     IGSFVAERGFKALQQAMSSGQHHHPRGVFYGGDAPTWSQQTLRQVLRQHATRCRRLAW
                     IDLHTGLGPNGHGERIFASRDDAAAFERAKAWWGPVTSIYDGSSTSALLTGMMFFAAY
                     DECPQAEYTGMALEYGTEPAPQVLDALRADQWLENHPDTDEQTRRAIKKQVRDAFYTD
                     TDEWKERIVEQALDASWGALRGLSGTAST"
     gene            2519..2842
                     /locus_tag="VFF72_03020"
     CDS             2519..2842
                     /locus_tag="VFF72_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin family protein"
                     /protein_id="HZV92158.1"
                     /translation="MQRRYRVLIGLGLAAVASVAAAQITFFENDGFRGRSFTTDRAVG
                     DFNRFGFNDRASSVIVRSGRWELCDDARFGGRCIMLRPGRYRSLSAMGLNDRISSVRP
                     AGRRR"
     gene            2924..4378
                     /gene="zwf"
                     /locus_tag="VFF72_03025"
     CDS             2924..4378
                     /gene="zwf"
                     /locus_tag="VFF72_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZV92159.1"
                     /translation="MSFDLVVFGGTGDLAWRKLMPALFQAFRHGQLPAGGRILAVSRQ
                     EISDDAYRAWLKERFAQVDDAKRPTDDEFARFAALLHHIRVDLSHPGDYRRLGDWLAG
                     RDGGAAEVVVMYLATGPDLFPVICGQLGAAQLNGPTVRVVLEKPLGRDLASAQEINRI
                     VGSVFREEQAFRIDHYLGKPSVQNLMALRFGNALFEPLWRRESIANIQITLAEAIGVG
                     TRGDFYSRTGALRDMIQNHALQLLTMIAMEPPSRNDADAIRDEKLKVLRSLKPFTAES
                     VVRDVVRGQYRAGSVAGRQVPGFLEEDKVPAGSRTETFVALRTEVGNWRWASVPFYLR
                     TGKRLATRDAQIVVNFRPAPHSIFPGVNPPNKLVINLQPEDGLELHLLAAKGAGQSEA
                     LSPVSLDLDFDKAFAENRVGAYERLLLDVIAGRLNLFVRSDEQEEAWRWVEPILAAWS
                     ADDAGPRPYMAGTWGPAAASALVARDGNAWSEEQ"
     gene            complement(4398..4580)
                     /locus_tag="VFF72_03030"
     CDS             complement(4398..4580)
                     /locus_tag="VFF72_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92160.1"
                     /translation="MSKTPKSSGESPERAALERSEKHAAEKQPGSFKEEATDEKIVEI
                     PPAGPGKKPIRGLDPK"
BASE COUNT          748 a         1671 c         1557 g          704 t
ORIGIN      
        1 gagcatcgcg caggtgtccg ggcaatcgtg cggacaggcg gcgcgaacga cggtttgcga
       61 catcacgaaa tgatggcata gcgggcgatc cgtcagccgc atcggctctc gaccgcgccg
      121 atccgcccgg ccacccggta cccacgccgg ggcatccccg caccccgctg cggcgcccct
      181 gcgcgggtaa actgcccctc ttccgggagc tgccatgcaa gtcatcgaat ccatcctggc
      241 cgacgcggcc cagatcgcgg cggtgcgccg cgacatccat gcccatcccg agctgtgctt
      301 caaggaagag cggacgtccg acctgatcgc caggcaactc accgaatggg gcatcccgat
      361 ccagcggggc ctgggcacca ccggcatcgt cgcgatcctg aagaacggca gcagcggccg
      421 cgccgtcggc ctgcgcgccg acatcgacgc gctgccgatc accgagcaca accagttcgc
      481 gcacaagagc acgcatgccg ggaagatgca cgcctgcggc cacgacggcc acacggcgat
      541 gctgctggcc gccgccaagc acctttcgaa gcaccgcaac tacgacggca ccgtctacct
      601 catcttccag ccggccgaag agggcggtgg cggcgcccgc gagatgatga aggacggcct
      661 cttcgagaaa ttcccgatgg aggcggtgtt cggcgcgcac aactggcccg gcatgaaggc
      721 cggacagttc gcgctcaaga gcggcccggc gttcgcctcg agcaacgagt tccacatcac
      781 cgtccgcggc aagggctcgc acgcggccat gcctcacctg ggcgtcgatc cggtgccggt
      841 ggcgtgccag atcgtcaacg cgttccagac catcatcagc cgcaacaagc ggccgatcga
      901 cgccggcgtg atttcggtga ccatgatcca cgccggcgag gcgaccaacg tcgtgcccga
      961 cagctgcacg atcgagggca cggtgcgcac cttcacgctc gaggtgctcg acctcatcga
     1021 aaagcgcatg aaggaggtcg tcgagcacac ctgcgcggcc ttcgacgcga cctgcgaatt
     1081 cgcgttcaag cgcaactatc cgccgaccat caaccatccg gccgagaccg agttcgcgcg
     1141 caaggtgatg caggacatgg tgggagccga gaacgtcctc gagttcgagc cgacgatggg
     1201 tgccgaagac ttcagctact tcctgctcga aaagcccggc tgctatttcc tgatcggcaa
     1261 cggcgacggc tcgcaccgcg aaggcggcca cggcatgggc ccgtgcatgc tgcacaaccc
     1321 gagctacgac ttcaacgacg acctgatccc gctcggcggc tcgctgtggg ttcgcctggc
     1381 cgaggcctgg ctggcgcccc gatgagcgcc gcggccggct tcgcgcagac ctacgccgag
     1441 gcgcgcgaga agttcctggc cgccgcgagg aatcgcggcc tgggcatcga gacgcacgtc
     1501 cacccgctgc ccggccgcga cggcgagatg ctggcgatgg acgtcgcgcg ggacggcgcc
     1561 gccgatgccg cctccgtgct catcgtcagc agcgcctgcc atggcgtcga gggcttctgc
     1621 ggttcgggcg tgcaggtcgc cctgctcggc gacgacgcgt ggcaccgcga ctgcgccgac
     1681 gccggcgtcg ccgtgctcta tgtgcacggc ctcaacccgt acggattctc gtggtggcgg
     1741 cggacgacgc acgaaaacgt cgacctcaac cgcaacttcc gcgatttcgc ccagcccctg
     1801 ccgcgcaacg agggctacga cgacctggcc gaactgatcg ttccggaagt ctggccgccc
     1861 gacgaagcga cgactcgggg gatcggcagc ttcgtcgccg agcgcggctt caaggccttg
     1921 cagcaggcga tgtcgagcgg ccagcaccac catccgcgcg gtgtcttcta tggcggcgac
     1981 gcgccgacat ggagccagca gacgctgcgc caggtgctgc gacagcatgc gacccgctgt
     2041 cgccgcctgg cctggatcga cctgcacacc ggactcggcc cgaacggcca tggcgagcgc
     2101 atcttcgcca gccgcgacga cgccgccgcg ttcgagcgtg ccaaggcgtg gtggggtccc
     2161 gtcacgtcga tctacgacgg ctcgtcgacc tcggcgctgc tcaccggcat gatgttcttt
     2221 gccgcctacg acgagtgtcc gcaggccgag tacaccggca tggccctgga atacggcacc
     2281 gagcctgcgc cgcaggtcct cgatgcgctg cgcgccgacc agtggctcga gaatcatccc
     2341 gacaccgacg agcagacccg gcgcgcgatc aagaaacagg tgcgcgatgc cttctacacc
     2401 gacaccgacg agtggaaaga gcggatcgtc gagcaggcgc tcgacgcttc atggggcgcg
     2461 ctgcgcggac tgagcggcac ggcctcgacc tgaagaccca cgcacaggag cgaaccccat
     2521 gcagcgacgc tatcgtgttc tcatcggcct gggactggcc gcggtggcca gcgtcgccgc
     2581 cgcgcagatc accttcttcg agaacgacgg ctttcgtggt cgatcgttca cgaccgaccg
     2641 ggcggtcggc gacttcaatc gcttcggctt caacgatcgc gcctcctcgg tgatcgttcg
     2701 atcgggccgc tgggagctct gcgacgacgc ccgcttcggc ggccgctgca tcatgctgcg
     2761 gccgggccgc taccggtctc tcagcgcgat gggtctgaac gaccgcatct cgtcggtgcg
     2821 gccggccggc cggcggcgct gacgacgccg gaacgaccac tccggggcgc gcgcttcgcg
     2881 gcaccccggc gcgccggccg gcccgccgac gtatcctgag tgcatgtcat tcgatctcgt
     2941 cgtcttcggc ggcacgggcg acctggcgtg gcgcaagctc atgccggccc tgttccaggc
     3001 ctttcgccac ggccagttgc ctgcgggcgg tcgcatcctc gcggtgtccc gccaggagat
     3061 cagcgacgac gcgtatcgcg cctggctgaa ggagcgattc gcccaggtcg atgacgccaa
     3121 gcggccgacc gacgacgagt tcgcccgctt cgcagcgctg ctgcaccaca tccgcgtcga
     3181 cctgtcgcat cccggcgact accgccgtct cggcgactgg ctcgcggggc gcgacggcgg
     3241 cgccgccgag gtcgtcgtca tgtacctggc gaccggcccc gacctgttcc cggtcatctg
     3301 cggccagctc ggcgcggcgc aactgaacgg gccgacggtg cgcgtggtcc tggaaaagcc
     3361 gctcggccgc gacctcgcga gcgcgcagga aatcaaccgc atcgtcggct cggtgtttcg
     3421 cgaggagcag gcatttcgca tcgaccacta cctcggcaag ccgtcggtgc agaacctgat
     3481 ggcgctgcgc ttcggcaacg ccctgttcga gccgctctgg cggcgcgaat cgatcgccaa
     3541 catccagatc acgctggccg aggcgatcgg cgtcggcacg cgcggcgatt tctacagccg
     3601 caccggggcg ctgcgcgaca tgatccagaa ccacgcgctg cagttgctga ccatgatcgc
     3661 gatggagccg ccgtcgagga acgacgccga cgcgatccgc gacgagaagc tgaaggtgct
     3721 tcgctcgctc aagccgttca cggccgagag cgtcgttcgc gacgtggtcc gcggccagta
     3781 ccgcgccggc agcgtcgccg ggcggcaggt gcccggcttt ctggaggaag acaaggtgcc
     3841 cgccggcagc cgcaccgaga ccttcgtcgc cttgcgcacc gaggtcggca actggcgctg
     3901 ggcgtcggtg cccttctacc tgcgcaccgg caagcgcctg gccacgcgcg acgcgcagat
     3961 cgtcgtcaac ttccggcccg ctccgcacag catctttccg ggcgtcaacc cgccgaacaa
     4021 gctcgtcatc aacctgcagc ccgaggacgg cctcgagctg cacctgctcg ccgccaaggg
     4081 cgccggccag tcggaggcgc tctcgccggt gtcgctcgac ctcgacttcg acaaggcctt
     4141 cgccgagaac cgcgtcggcg cttacgagcg actgctgctc gacgtcatcg ccggccggct
     4201 caacctcttc gtgagaagcg acgagcagga agaggcatgg cgctgggtcg agccgatcct
     4261 ggccgcgtgg tctgccgacg acgccggacc gcggccctac atggcgggaa cgtgggggcc
     4321 ggccgcagcc agcgcgctgg tcgctcgcga cggcaacgcg tggtcggaag agcagtagcg
     4381 acgaatcgcg gggggttcta tttcgggtcg aggccgcgga tcggcttttt tcccgggccg
     4441 gcaggcggga tctcgacgat cttctcgtcg gtcgcctctt ccttgaagct tccgggctgc
     4501 ttttctgcgg cgtgcttctc ggaccgctcc agcgccgcgc gctcgggcga ctcgccggac
     4561 gacttcgggg tcttgctcat tcgaggttcc tcgttcgctg cgttgccgcg gcatgaacgc
     4621 catcgtcgcg ccgaggcgcg gtcgccgcga gccggcaacg gcgcgtccgg ctgtgggaaa
//
LOCUS       DASRWS010000168         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_644448, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000168 DASRWS010000000
VERSION     DASRWS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644448"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..698)
                     /locus_tag="VFF72_03035"
     CDS             complement(75..698)
                     /locus_tag="VFF72_03035"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZV92161.1"
                     /translation="MFTGIVSGVGRIVEAKPLGDRPDFGHRLVLAAPRGFLGDVRLGD
                     SIALSGACMTVVSLDDDADRFEIEISAESLACTTGLDAPGEVNLEKAMRVDGRLDGHL
                     VAGHVDGVGRVVGVTPIGESRELRIDAPASLSRFLAAKGSVAVDGVSLTVNRVDDSPS
                     GCRIVINLIAHTVASTSLRNLAAGSRVNLEVDLVARYVERMLGMRGA"
     gene            complement(860..1930)
                     /gene="ribD"
                     /locus_tag="VFF72_03040"
     CDS             complement(860..1930)
                     /gene="ribD"
                     /locus_tag="VFF72_03040"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZV92162.1"
                     /translation="MREALALAAEAIGRSDPNPRVGCVIVGAGGRVIGRGSTQRAGDA
                     HAEVMALRDAATRGADVRGATVFVSLEPCSHHGRTPPCADALVAAGVGRVVMAMRDPN
                     PLVAGRGAARLAAAGIVVEEGVCEREARELNVGFVSRMERGRPWLRMKAAVSLDGRSA
                     LANGVSQWITGDAARTDGHAWRRRAGATLTGVGTVLEDDPRLDVRLVPTAAQPLRVIV
                     DSRLETPPRARIVEPPGDVLVFAATDDGARSDALRQRGVEIVLLPSPAGKVDLPAMLA
                     ELGRRGINELHVEAGEKLNASFLREGLVDELLVYVAPRLLGEGRGLAALGPFERLDEA
                     LGFCFVDIAPVGDDLRLRLRRA"
     gene            complement(2011..>2864)
                     /locus_tag="VFF72_03045"
     CDS             complement(2011..>2864)
                     /locus_tag="VFF72_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92163.1"
                     /translation="TLETVIARVDPAYSGALAVGTGNHAARGAFGRSPAIPASARSLG
                     GGRSAWKPTPASPVVLVFDDASGQVVAQCAGVTTAMRDLVADELTGCVDGHWFLLSGT
                     VRFSAAIPPVANDANSPSLPFSVALAMTGGGEAVAPQCVVEAMKTVRRIDDGGLHLDA
                     VDVAATPQSEGFDNWEDTGERFAVYHCVVAPRADGRWSGRSTLAPLEWSLGTSSTDWR
                     VCRFAVDRDGSGAIDANIEHPADYHDVDTTLNGQNFLVVHGDQDCPVAAAGDPLAEAT
                     NTVPHQP"
BASE COUNT          433 a          982 c         1058 g          391 t
ORIGIN      
        1 cgagctccgg aatggcagcg atggacatca tgaaaattgt aggcgccggg gcgcttcgcg
       61 gtcgtggagg ggattcatgc gccgcgcatg ccgagcatgc gctcgacata gcgggcgacc
      121 aggtcgactt cgagattgac cctggagccg gccgcaagat tgcgcaggct cgtgctggcg
      181 acggtgtggg cgatcaggtt gatgacgatc cggcagcccg atggcgaatc gtcgacgcgg
      241 ttcacggtga ggctgacacc atcgaccgcg accgagccct tggcggcgag gaagcgcgaa
      301 agcgacgctg gcgcatcgat gcgcagctcg cgcgactcgc cgatcggcgt gacgccgacg
      361 acgcgaccga cgccgtcgac gtggccggcc acgagatgac catcgagtcg gccatcgacc
      421 cgcatcgcct tctcgaggtt cacttcgccg ggggcgtcga gcccggtggt gcaggccagg
      481 ctctccgcgg agatctcgat ctcgaagcgg tcggcgtcgt cgtcgagcga gaccacggtc
      541 atgcaggcac ccgacaaggc gatgctgtcg cccagcctga cgtcgccaag gaagccgcgg
      601 ggcgccgcga gcacgaggcg gtggccgaag tccggacgat cgcccagagg tttcgcttcg
      661 acgatgcggc cgacgccgct gacgatgccg gtgaacacga tgcgccgatt gtccgcccgc
      721 ggtcaaaggt tcggcgtcgc ggaggcgtca tgtcgccatc gccttggtcg gcgcgagacg
      781 gggccatggc gggcggcgcc gggtggcacc cggcgtcctg cctgctggcg cgggtcgagg
      841 ccagccggag gatggcgcct caggcgcggc gcaggcgcag acgaagatcg tcgccgaccg
      901 gggcgatgtc gacgaagcaa aatccgaggg cctcgtcgag gcgctcgaac ggccccagcg
      961 cggcgaggcc gcggccttcg ccgagcaggc gcggcgccac gtagacgagc agctcgtcga
     1021 ccagtccctc gcgcaggaac gaggcattga gcttctcgcc ggcttcgacg tgcagctcgt
     1081 tgatgccgcg ccggccgagc tcggcgagca tcgccggaag gtccaccttg ccggccggcg
     1141 agggcaggag aacgatctcg acgccgcgct gtcgcaaggc gtcgctgcgg gccccgtcgt
     1201 cggtcgcggc gaagacgagc acgtcgcccg gcggttcgac gatgcgcgcc cggggcggtg
     1261 tctcgagccg cgaatcgacg atcacgcgca gcggctgggc tgccgtcggc acgaggcgca
     1321 cgtcgagtcg cggatcgtct tcgagcacgg ttccgacgcc ggtcagcgtc gcccccgcac
     1381 gccgccgcca ggcgtgcccg tcggtgcgcg cggcgtcgcc ggtgatccat tggctgacgc
     1441 cgttggccag cgcgcttcga ccgtcgagcg agacggcggc cttcatgcgc agccacgggc
     1501 ggccgcgctc catgcgcgac acgaagccga cgttgagctc gcgggcctcg cgctcgcata
     1561 ccccctcctc gacgacgatg cccgcggccg cgaggcgcgc cgcgccgcgg ccggcgacga
     1621 gcgggttggg gtcgcgcatc gccatcacga cgcgcccgac acccgcggcc acgagcgcgt
     1681 cggcgcacgg cggcgtccgg ccatgatgcg agcagggctc gaggctgacg aagacggtgg
     1741 cgccgcgcac gtcggcgccc cgggtggcgg cgtcgcgcaa ggccatgacc tcggcgtgcg
     1801 cgtcgccggc gcgctgggtg ctgccgcggc cgatcaccct gccgcctgcg ccgacgatga
     1861 cgcagccgac gcgcgggttc ggatccgacc ggccgatcgc ctcggcggcg agcgcgagcg
     1921 cctcgcgcat cggcgcgatg acgctcgaat cgtcaccgaa cgatggttcg tcgggtgccg
     1981 gcgctggcgg gctgggagtc atgcgtttct tcagggctgg tgcggcacgg tgttcgtcgc
     2041 ctcggcaagc ggatcgccag cggcagcgac aggacagtcc tggtcgccat gcacgacaag
     2101 gaaattctgc ccgttcagcg tcgtgtcgac atcgtggtag tcggcggggt gctcgatgtt
     2161 cgcatcgatc gcgccgctgc cgtcgcggtc gaccgcgaaa cggcagacgc gccagtcggt
     2221 gctgctcgtg ccaaggctcc attcgagcgg cgccagcgtc gatcgacccg accagcgccc
     2281 gtcggcgcgc ggagcgacga cgcagtggta gacggcgaat cgttcgccgg tgtcctccca
     2341 gttgtcgaag ccctccgact gcggcgtcgc ggcgacgtcg acggcatcca ggtgaagccc
     2401 accgtcgtcg atgcggcgca ccgtcttcat cgcttcgacc acgcattgcg gggcgactgc
     2461 ctcgccgcct ccagtcattg ccagagcgac tgaaaaaggc agcgaaggcg aattcgcatc
     2521 gttcgcgacg ggtgggatgg cggcactgaa tcgcacggtg ccggagagga ggaaccagtg
     2581 gccgtcgacg cacccggtga gctcatcggc gacgagatcg cgcatcgccg tcgtcacacc
     2641 ggcgcactgg gcgacgacct ggccgctcgc atcgtcgaag acgagaacga cagggctcgc
     2701 gggtgtcggc ttccacgcac tgcggccgcc gcccaacgaa cgcgccgatg ccggaatcgc
     2761 cggtgaccgg ccgaaggcac cgcgagccgc gtggttgccg gtgccgacgg cgagcgctcc
     2821 ggaataggct ggatcgacgc gggcgatcac cgtctcgagc gtcg
//
LOCUS       DASRWS010000169         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_354980, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000169 DASRWS010000000
VERSION     DASRWS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354980"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFF72_03050"
     CDS             <1..1247
                     /locus_tag="VFF72_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZV92164.1"
                     /translation="EPPPGGGFGFCDVVFGWDMTDATYDNATHTGWQHGFPDALARLD
                     LATQRSVPWDGNVPFFLGEFVATDGSPLPICPRQTLKRVLARASKMGFSVMAGLEFEW
                     FNFLETPQTWAAKQGVAPTPLTPGMFGYSLLRMNRNREFMNALMDEMAAFGVPIEGLH
                     TETGPGVYEVAIAFSEALAQADRAILFKTGAKEIGARFGIMPSFMAKWSQQYPGCSGH
                     VHQSLREGPDGKINLFHDASSPTGMSPLFESYVAGQVACLMEFAPMFWPTINSYKRLV
                     DGFWAPVKPTWGIDNRTASFRVLAGSPKSTRLETRCPGADINPYIAMAAVIAAGLEGI
                     EKGWKLSAPPITGTNAGAEHVPRAPRTLIETTRIFQQSRVARDWLGDDFVDHFAATRD
                     WEWRRWLDGVTDWELKRYLEII"
     gene            1318..2925
                     /locus_tag="VFF72_03055"
     CDS             1318..2925
                     /locus_tag="VFF72_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92165.1"
                     /translation="MKSTSRPVRPGRAWRALAGALLLVVPLLGMAKPVRIATAFDPQT
                     MDPHALALLYHSRIVYQIYESLVGRDEQFRLEPALAVSWKAIDPLTWRLRIRQGVVFH
                     DGTPFTVDDAVFSIRRAMTPPSQRAFQLRGVRDAVKVDGQTLDIRLDAPDAVLPEKLF
                     ALPMMSRAWATLHRVEVAQDFNGKQETFAVRHANGTGPYRLERYEPDSRVVLRKNPRW
                     WGWSDRRSGNVDEVDWLVIRSDATRLAALISGEVDMVLDPPIPDVARLKGEAALNVLQ
                     TEDLGEQYLVFDVARDELVGSDVKGRNPFKDLRVRQAVYHAINVDLIARKVLRGLAVP
                     TGAYLSKRVDGSPPELDRRLAFDPAKARSLLASAGYPDGFSVTLDCVNVAWREAVCQA
                     MTAMLTQVGIRATLDSTPTNQFFPKLSGATTSLAEFGWTPTLDAWASLNALFHTWDRT
                     GLGTFNAGRYRNPKVDALIDALRVEPDLARRRAMVGDVLRIVAQDLPSIPLYRRTLNW
                     AIVRPLHAVQWPNDTLELRWLRWLRWQ"
     gene            2963..>3272
                     /locus_tag="VFF72_03060"
     CDS             2963..>3272
                     /locus_tag="VFF72_03060"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZV92166.1"
                     /translation="MTLHHFSFPTAIEFGAGARRSVAPHLAERGVARPLVVTDRGLAA
                     LPLIAEMMQSLSSSRLQAALFSGVSGNPTASQVMAAAAGYRSHGADCVVGIGGGAALD
                     V"
BASE COUNT          505 a         1136 c         1081 g          550 t
ORIGIN      
        1 ccgaaccgcc gcccggcggc ggcttcggct tttgcgacgt cgtcttcggc tgggacatga
       61 ccgacgccac ctacgacaac gcgacgcaca ccggttggca gcacggcttt cccgatgcgc
      121 tggctcgcct cgacctcgcg acccagcgca gcgtcccgtg ggacggcaac gtcccgttct
      181 ttctcggcga attcgtcgcc accgatggtt cgcctttgcc gatctgcccc cgccagacgc
      241 tcaagcgggt gctcgcgcgg gcctcgaaga tgggcttttc ggtgatggcc gggcttgagt
      301 tcgagtggtt caacttcctc gagacgccgc agacctgggc cgccaagcag ggcgtggcac
      361 cgacgccgct gacgccgggc atgttcggct actcgctgct gcggatgaac cgcaatcgcg
      421 aattcatgaa cgcgctgatg gacgagatgg cggctttcgg cgtgccgatc gaagggttgc
      481 acaccgaaac ggggccgggc gtctacgaag tggcgatcgc cttcagcgag gcgctggcgc
      541 aggccgatcg cgccatcctc ttcaagaccg gggccaagga gatcggtgct cgcttcggga
      601 tcatgcccag cttcatggcc aagtggagcc agcaatatcc cggctgcagc ggtcacgtcc
      661 accagagcct gcgcgaaggc cccgatggca agatcaatct ctttcacgat gcgagctcgc
      721 cgaccggcat gagccccctc ttcgaaagct acgtcgccgg ccaggtcgcc tgcctgatgg
      781 agttcgcgcc gatgttctgg ccgacgatca acagctacaa gcggctcgtc gacggcttct
      841 gggcgccggt caagccgacc tggggaatcg acaaccggac ggcgagcttt cgggtcctcg
      901 ccggcagccc gaaatcgact cggctcgaga cacgttgccc cggtgccgac atcaacccct
      961 acatcgcgat ggcggcggtg atcgcggccg gcctcgaagg catcgagaag ggatggaagc
     1021 tgagtgcccc gccgatcacg ggaacgaatg caggcgccga gcatgttccg cgcgcgccgc
     1081 gcaccctgat cgagacgacc aggatcttcc agcagtcgcg ggtggcccgc gactggctcg
     1141 gcgatgattt cgtcgaccac ttcgccgcga cccgcgactg ggagtggcgc cggtggctcg
     1201 acggcgtcac cgactgggag ctgaagcgct atctggagat catctgagcc gaagcggtgg
     1261 cgctcctggc gcgccatcca cccctgctgc gcgacccgaa ccaccgaagc aacgccgatg
     1321 aagtccacgt cacgtcctgt tcgaccaggc cgcgcatggc gggcactggc cggtgccttg
     1381 ctcctggtgg tgccgctgct gggcatggcc aagccggttc gcatcgccac cgcgttcgat
     1441 ccgcagacga tggatccgca cgcgctggca ttgctgtatc actcgcggat cgtctatcag
     1501 atctacgaat cgctggtcgg ccgcgatgag cagtttcgcc tcgagccggc gcttgccgtg
     1561 tcgtggaagg cgatcgaccc gttgacctgg cgcctgcgga tccgccaggg cgtcgtcttt
     1621 cacgacggca cgcctttcac cgtcgacgat gcggtctttt ccatccggcg ggcgatgacg
     1681 ccgccatcgc agcgggcgtt ccagctgcgc ggcgttcgcg atgccgtgaa ggtcgacggg
     1741 cagaccctcg acatccgcct cgacgctccc gacgccgtcc tgcccgagaa gctgttcgcg
     1801 ctgccgatga tgagccgagc ctgggcgacg ttgcaccggg tcgaggtcgc gcaggatttc
     1861 aacggcaagc aggaaacgtt tgccgttcgc cacgccaacg gcaccggtcc gtaccggctc
     1921 gagcgctacg agcccgactc gcgcgtcgtg ctcaggaaaa atccgcgctg gtggggatgg
     1981 agcgacaggc gctcgggcaa cgtcgacgag gtcgactggc tcgtcatccg atccgacgcg
     2041 acgcggctgg cggcgctgat ctcgggcgag gtcgacatgg tgctcgatcc gcccattccc
     2101 gacgtcgcgc gactgaaggg cgaggccgcc ctgaacgtgc tgcagaccga ggacctgggc
     2161 gagcagtacc tcgtcttcga cgtcgcgcgc gacgagctcg tcggctccga cgtcaagggc
     2221 cgcaatccgt tcaaggacct tcgcgttcgg caagccgtct accacgcgat caatgtcgac
     2281 ctgatcgccc gcaaggtcct gcgcggcctc gccgtgccga ccggcgccta cctgtcgaag
     2341 cgggtcgatg gatcgccgcc ggagctcgac cgccggctgg cgttcgaccc ggcgaaggcg
     2401 cgcagtctcc tcgcgtcggc gggctatccg gacgggtttt cggtcacgct cgattgcgtg
     2461 aacgtcgcct ggcgcgaagc cgtctgccag gcgatgacgg cgatgctgac ccaggtcggc
     2521 atccgggcca cgctcgactc gacgccgacg aaccagttct ttccgaagct gagcggtgcg
     2581 acgaccagtc tcgccgaatt cggctggacg ccgacgctcg acgcctgggc atcgctcaac
     2641 gcccttttcc acacctggga ccgcaccggc ctgggcacgt tcaacgccgg tcgctaccgc
     2701 aacccgaagg tcgacgcgct gatcgacgcc ttgcgcgtcg agcccgacct ggcgcgacgc
     2761 cgggcgatgg tgggcgacgt gctgcggatc gtcgcgcagg acctgccgtc gatcccgctc
     2821 tatcgccgca cgctcaactg ggcgatcgtt cggccgctgc acgccgtgca atggccgaac
     2881 gacactctcg aactgcgctg gctgcgctgg ctgcgctggc aatgagcgcg cgacttccct
     2941 gcgaacttcg acccacaccg cgatgacgtt gcaccacttt tcgtttccga ccgcgatcga
     3001 gttcggtgcc ggtgcccgcc gcagcgtggc gccgcacttg gctgaacgtg gcgttgcccg
     3061 cccgctcgtc gtcaccgacc ggggcctcgc tgcgttgccc ctgatcgccg agatgatgca
     3121 gagcctgtcg tcgagccggc tgcaagctgc gctcttctcc ggcgtgtcgg gcaacccgac
     3181 ggcgagccag gtcatggcgg ccgccgccgg ctatcggtcg cacggtgctg actgcgtcgt
     3241 cggcatcggc ggcggcgcgg cgctcgacgt cg
//
LOCUS       DASRWS010000170         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2084386, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000170 DASRWS010000000
VERSION     DASRWS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084386"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..>2063
                     /locus_tag="VFF72_03065"
     CDS             37..>2063
                     /locus_tag="VFF72_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZV92167.1"
                     /translation="MQIRRVTEGSAKVADRLRREVLYYIAIAAPAAPQIEAVQRAYQL
                     AALLPTPEALDADIVRVKPLLREARDQLASAKDAWLKAASGRAESLPKLTQLLASAHA
                     KAQEIGHPALAKLTAALAERLAAMPPQGASEPVAMEFATALLLAESAFENFGNLSPDF
                     ASQVDAMLARLAAVHTGEAPAQGAPALDEMSRRAQERVLLGQVMREVQANLRHMEQVL
                     DAFFRDHTRRLELGGLTRDSQQIRGALRMLELDDADRLLALCQAQIESYADPATAVDE
                     DGLELLAESLSGLGFYIDAVLHQRPDRERIIAPLIAKRLGEAPRAEVAEPQSVEESVA
                     ELRAALPRLLADVREAPADASARSELRGKLVSLRDDADLIGDAELATQVKAAIREIDG
                     GGAEAALAASVDLIVESGATPAPALSEETQRLLATDARALDAEILDIYLTEADEVLDA
                     VDENLRVLQHSPADRDALVTVRRQFHTLKGSGRMVGLTELGELAWGVERIHNRLLEEE
                     RRVTPAFLALIGVAATSFRQWVRELRESGRFATDASPLLSAMRAVEAEWPGAVPPALS
                     LPPVAPVKPVPVAAEHPIGPVSPPRTASPAGGIEAFLPFEGDAGGSIEFPASNDEAAS
                     IESRALGNSLDAEFTVPVDEMAGDEVAADDAPSAHELPRLPDLELVTFAELG"
BASE COUNT          321 a          730 c          724 g          288 t
ORIGIN      
        1 tcgttcggcg cgaagcagct cgtcgcgcgg atcgacatgc agattcgccg cgtcaccgaa
       61 ggcagcgcga aggtggccga ccggctgcgc cgcgaggtgc tctactacat cgcgatcgca
      121 gcgcctgcgg caccgcagat cgaggccgtg cagcgcgcgt atcagctcgc ggcgctgctg
      181 cccacccccg aagcgctcga tgccgacatc gtgcgcgtga agccgctgct gcgcgaggcg
      241 cgcgaccagc tcgcgagtgc caaggacgcg tggctgaagg cggcgtcggg acgtgccgaa
      301 agcctgccga agctcacgca gctgcttgcc tccgcacacg caaaggcgca ggagatcggc
      361 catccggcgc tggctaagct caccgcagcg ctcgccgagc gtctcgccgc gatgccgccg
      421 cagggcgcat ccgagccggt ggcgatggaa ttcgcaacgg cgttgctgct cgccgaaagc
      481 gcattcgaga acttcggcaa cctgtcgccc gatttcgcga gccaggtcga cgcgatgctg
      541 gcgcgcctcg ctgccgtgca caccggagag gcaccggcgc agggcgcgcc ggcgctcgac
      601 gagatgtcgc gccgcgcgca ggagcgcgtg ctgctcggtc aggtgatgcg cgaagtgcag
      661 gcgaacctgc ggcacatgga gcaggtgctc gacgcgttct tccgcgacca cacccgccgg
      721 ctcgagcttg gcggcctcac gcgcgacagc cagcagatcc gcggcgcgct gcgcatgctc
      781 gagctcgacg acgccgaccg gctgctggca ctttgccagg cgcagatcga aagctacgcc
      841 gatccggcaa ccgccgtcga cgaggacggc ctcgagctgc tcgccgaatc gctgtcgggt
      901 cttggcttct acatcgacgc ggtgctgcat cagcggcccg atcgcgagcg gatcattgcc
      961 ccgttgattg cgaagcgtct cggcgaagcg ccgcgcgccg aagtcgccga gccgcagtcg
     1021 gtcgaggagt cggtcgccga actgcgtgcg gcgctgccgc gcctcctggc cgacgtgcgc
     1081 gaggccccgg ccgacgcgtc ggcacgcagc gaactgcgcg gcaagctcgt gagcctgcgc
     1141 gacgacgccg acctcatcgg cgacgccgag ctcgccacgc aggtgaaagc ggcgatacgc
     1201 gaaatcgacg gcggcggcgc ggaggcggca cttgccgcga gcgtcgacct catcgtcgag
     1261 tcgggtgcca cgccggcccc ggcgctgtcg gaagaaacgc agcgtctgct tgccaccgac
     1321 gcgcgcgcac tcgacgccga gatcctcgac atctacctga cggaagcgga cgaggtgctc
     1381 gacgccgtcg acgagaacct gcgcgtgctc cagcattctc ctgccgatcg cgacgcgctc
     1441 gtcaccgtgc gccggcagtt ccatacgctg aaaggcagcg ggcggatggt cggcctgacc
     1501 gagctcggcg agctcgcctg gggcgtcgag cgcatccaca accggctgct cgaagaggag
     1561 cggcgcgtga cgccggcgtt cctagcgctg atcggcgtcg cggccacctc gttccgacaa
     1621 tgggtgcgcg agctccggga aagcgggcgt tttgcgaccg acgcatcgcc gctgttgtcg
     1681 gccatgcgcg ccgtggaagc cgaatggccg ggtgcagtgc cgccggcgct ttcgctgccg
     1741 ccggtcgcac cggtcaagcc cgtgcccgtc gccgcagagc atccgatcgg cccggtgtcg
     1801 ccgccgcgca ctgcatcgcc cgccgggggc atcgaagcct tcctgccatt cgagggtgac
     1861 gccggcggca gcatcgaatt tcccgcgtcg aacgacgagg cggcgagcat cgaatcgcgc
     1921 gcactcggca acagcctcga cgccgaattc acggtgcccg tcgacgagat ggcgggcgac
     1981 gaggttgcgg ccgatgacgc gccatcggcg cacgagctgc ctaggcttcc ggatctcgag
     2041 ctcgtcacgt ttgccgagct cgg
//
LOCUS       DASRWS010000171         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_646032, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000171 DASRWS010000000
VERSION     DASRWS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646032"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="serA"
                     /locus_tag="VFF72_03070"
     CDS             <1..817
                     /gene="serA"
                     /locus_tag="VFF72_03070"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZV92168.1"
                     /translation="WIKSAEHAYEVRGKTLGIVGYGHIGTQIGVLAEQLGMSVVYRDI
                     EAKLPLGNARETADLDELLAISDVVSLHVPETPATRSMIGAAQLAAMKPGSHLINASR
                     GSVVDIDALADALERRHLHGAAIDVFPVEPQGNDTAFTSPLTRFDNVILTPHIGGSTS
                     EAQVNIGKEVAAKLIRYGNNGSTTSAVNFPEVALPEHTGRSRLLHIHRNVPGVLAHVN
                     ERLSAAGINIAAQFLSTNEEVGYVVIDVDAAASLPALSELCGVPATIRCRILY"
     gene            938..1195
                     /locus_tag="VFF72_03075"
     CDS             938..1195
                     /locus_tag="VFF72_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HZV92169.1"
                     /translation="MSTPHTHLFADQTYPFDARGVAKRFRHAAIFGALDSLHPGETMH
                     FVNDHDPLPLLDQLRARYGDAIGIEYRQRDPGAIAIDFVRR"
     gene            1232..1570
                     /locus_tag="VFF72_03080"
     CDS             1232..1570
                     /locus_tag="VFF72_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZV92170.1"
                     /translation="MAITLTEAAARQINASIAKRGHGFGLRVGVKAVGCSGLTYTYEI
                     ADEARPGEAVFEGHGARLLTDDKALTFIDGARFDFVTEGLKQAFSVDNPNVDSTCGCG
                     ESFNVKVGVA"
     gene            1581..>3012
                     /gene="sufB"
                     /locus_tag="VFF72_03085"
     CDS             1581..>3012
                     /gene="sufB"
                     /locus_tag="VFF72_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZV92171.1"
                     /translation="MSALLQDLVNRPYKHGFVTDIESDVAAKGLTEDTIRLISAKKNE
                     PEWLLEFRLKAFRHWLTMTEPEWATVKYPKIDFQAISYYSAPKPKAKLASMDEVDPEL
                     LRTFEKLGVPMHERAALAGVAVDVIFDSVSVTTTYKARLAEVGIIFGSISEAVQNHPE
                     LVRKYLGTVVPPGDNYYAALNSAVFTDGSFCYIPKGVKCPMDLSTYFRINTEDTGQFE
                     RTLIVAEEGASVSYLEGCTAPKFDTNQLHAAVVELVALDRADIKYSTVQNWYAGDENG
                     LGGIYNFVTKRGMCRGKASRISWTQVETGSAITWKYPSCILLGDDSVGEFYSVAVTNH
                     HQQADTGTKMIHIGRNTRSTIVSKGISAGSSNNSYRGLVKVMPTAHDARNYSQCDSML
                     IGAHCSANTFPYIQVRNPEAKVEHEASTSKIGEDQLFYFAQRGIGAEEAVSMIINGFC
                     KDVFRQLPMEFAVEATKLLGVKLEGSV"
BASE COUNT          568 a         1065 c          909 g          470 t
ORIGIN      
        1 ctggatcaag agcgccgagc acgcctacga agtgcgcggc aagaccttgg gcatcgtcgg
       61 ctacgggcac atcggcacgc agatcggcgt tctcgccgaa caactcggca tgagcgtcgt
      121 gtatcgcgac atcgaggcga agctgccgct cggcaacgcc cgcgagaccg ccgacctcga
      181 cgagttgctt gccatctcgg acgtcgtcag cctgcacgtg cccgagacgc cggcgacgcg
      241 gtcgatgatc ggcgcggcgc agctcgccgc gatgaagccc ggaagccatc tgatcaatgc
      301 ctcgcgcggc agcgtcgtcg acatcgatgc gctcgccgac gcgctcgagc gccggcatct
      361 gcacggcgcg gcgatcgacg tcttcccggt cgagccccag ggcaacgaca ccgcgttcac
      421 ctcccccctg acacgcttcg acaacgtcat cctgacgccg cacatcggcg gctcgacgtc
      481 ggaggcacag gtcaacatcg gcaaggaagt ggccgccaag ctgatccgct acggcaacaa
      541 cggctcgacg acgtcggccg tcaacttccc cgaggtcgcg ctgcccgagc acaccggccg
      601 cagccggctg ctgcacatcc accggaacgt gccgggcgtg ctggcgcacg tcaacgaacg
      661 cctgtcggcg gccgggatca acatcgccgc gcagttcctg agcaccaacg aggaggtcgg
      721 ctacgtggtc atcgacgtcg acgccgcggc cagcctgccc gctctgagcg agctgtgcgg
      781 cgtcccggcg acgatccgct gccgcatcct ctactgacgg gcccgccaat gcttgccttt
      841 cgcacgtttg ccctccatct gatgcggtcg cccgccgcat tcgcggcgcc gtgccggtcg
      901 ttcgccgacc gccgacaatc ctgaaagggc acgtaccatg tcgacgcctc acacgcatct
      961 cttcgccgat cagacctatc cgttcgatgc gcgcggcgtc gccaagcgct tccgccacgc
     1021 cgccatcttc ggagcgctcg attcgctgca tcccggcgag acgatgcact tcgtcaacga
     1081 ccatgatccc ctgccgctcc tcgaccagct ccgggcgcgc tacggcgacg ccatcggcat
     1141 cgagtaccgc cagcgagatc caggcgccat cgccatcgat ttcgtgcgcc gctgatttca
     1201 cccatccttt tcgcttcgaa agacccgagc catggccatc acactgaccg aagccgccgc
     1261 ccgacagatc aacgccagca tcgccaagcg cggccacgga ttcggcctgc gcgtcggcgt
     1321 caaggccgtc ggttgttcgg ggctcaccta cacctacgag atcgccgacg aggcgcgccc
     1381 cggcgaagcc gttttcgagg gccacggcgc ccgcctgctg accgacgaca aggcgctgac
     1441 cttcatcgac ggcgcgcgat tcgacttcgt caccgaggga ctcaagcagg ccttcagcgt
     1501 cgacaacccc aacgtcgaca gcacctgcgg ctgcggcgag agcttcaacg tgaaggtcgg
     1561 ggtcgcctga ggaatcgccc atgagtgccc tgctgcaaga cctcgtcaac cggccctaca
     1621 agcacggctt cgtcaccgac atcgagtcgg acgtcgccgc caagggcttg accgaggaca
     1681 ccatccggct gatctcggcg aagaagaacg agcccgaatg gctgctcgag ttccgcctca
     1741 aggcgttccg gcactggctg acgatgaccg agcccgaatg ggccaccgtc aagtatccga
     1801 agatcgattt ccaggcgatc agctactact cggcgcccaa gcccaaggcc aagctcgcga
     1861 gcatggacga agtcgatccc gaactgctgc gcaccttcga gaagctcggc gtgccgatgc
     1921 acgaacgcgc ggcactggcc ggcgtcgcgg tcgacgtgat cttcgacagc gtctccgtca
     1981 ccaccaccta caaggccagg ctcgccgagg tcggcatcat cttcggctcg atctccgagg
     2041 ccgtgcagaa ccatcccgag ctcgtgcgca agtacctggg cacggtcgtg ccgcccggcg
     2101 acaactacta cgccgcgctc aattccgcgg tcttcaccga cgggtcgttc tgctacatcc
     2161 ccaagggcgt gaaatgcccg atggacctgt cgacctattt ccggatcaac accgaagaca
     2221 ccgggcagtt cgagcgcacc ctgatcgtcg ccgaggaagg cgcgtcggtg tcgtacctcg
     2281 aaggctgcac ggcgccgaag ttcgacacca accagctgca tgcggcggtc gtcgagctgg
     2341 tcgcgctcga ccgcgccgat atcaagtact cgacggtgca gaactggtac gccggcgacg
     2401 agaacgggct gggcggaatc tacaacttcg tcaccaagcg cggcatgtgc cgcggcaagg
     2461 cgtcgcgcat ctcgtggacg caggtcgaga ccggctcggc gatcacgtgg aaatatccgt
     2521 cgtgcatcct gctcggcgac gactcggtgg gcgagttcta ttcggtcgcg gtgaccaacc
     2581 atcatcaaca ggccgacacc ggaacgaaga tgatccacat cggcaggaac acgcgcagca
     2641 cgatcgtcag caagggcatc tcggcgggca gctcgaacaa cagctatcgc gggctggtca
     2701 aggtcatgcc gacggcccac gacgcacgca actactcgca gtgcgactcg atgctgatcg
     2761 gcgcgcattg cagcgccaac acctttccct acatccaggt gcgcaacccc gaagcgaagg
     2821 tcgagcacga ggcgtcgacc tcgaagatcg gcgaagacca gctcttctat ttcgcgcagc
     2881 gcggcatcgg cgccgaggag gccgtctcga tgatcatcaa cggcttctgc aaggacgtct
     2941 tccgccagtt gccgatggag ttcgccgtcg aggcgaccaa gctgctcggc gtcaagctcg
     3001 aagggagcgt cg
//
LOCUS       DASRWS010000172         3778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_68875, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000172 DASRWS010000000
VERSION     DASRWS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68875"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFF72_03090"
     CDS             <1..753
                     /locus_tag="VFF72_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92172.1"
                     /translation="LVLYLRRAHKEAVGADNFLALGLVGLAFGAASLASANTFLAVFA
                     AGFALRRLELQSPRPRRHRREGATQREAALAKADASAKAIAAEVDDPHAAIARVGRLA
                     TDPRHAPAYMTHAVREFNEQIQRIGEVAAVVAIGMLLWAVDWHEVSWGFVAVLLVVVR
                     PLSVAIGLFASRTSLPQRGLTAWFGIRGVGSLYYLVFAIHQGLRPSLASTLAALVLGS
                     VVVSIVVHGISVTPLMALYERRKRRRGRARDA"
     gene            834..3293
                     /locus_tag="VFF72_03095"
     CDS             834..3293
                     /locus_tag="VFF72_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HZV92173.1"
                     /translation="MKEKSAPGEVWFAGRGWQPFAFQREVWKAMRAGRSGLLHATTGS
                     GKTYAVWFGAIERAVALDVPLRGAHAPSLGVLWLTPMRALAADSARAIRASIDDLGLS
                     WTVGIRTGDTASAERAKQDRRMPTALVTTPESLSLMLTRESARDDLSGVHTVIVDEWH
                     ELMGSKRGVQVQLAIARLRRWCPGLVVWGLSATLGNLREAMQVLLGDDDGELVQGRID
                     KALVIDTLLPATPGRFSWGGHLGKQMQHPVVEEIERSSTTLVFVNMRSQAEAWYQLIL
                     AEKPAWAGLVALHHGSLDKEVRDWVEQGLKQGTLKAVVATSSLDLGVDFLPVERVLQI
                     GSAKGVARLLQRAGRSGHAPGRASRITLVPTNTLELIEAVAARRAAAAGRIEKRATHD
                     KPFDVLVQHLVTVALGGGFTADALFDEVRTAWAFRKLTRAEFQWALDFVVQGGDSLQA
                     YPEYHRVVDVEGIYRVPNRAIARRHRLGVGTIVSDSSMQVKYLSGGRIGVVEEGFVAR
                     LRKGDCFVFAGRTLEFVRAEEMTCYVKRADGRKAAMISWAGAKMPLSSELADSVMEVL
                     ADAAQGDFFDPEMEAARPMLETQMQLSRIPTPKTLLIEKLDSREGQHLFLYPFAGRNA
                     HIGLGSLLAWRLAKDAPNTFSIVINDYGLELLSARPVDPAPLVDQSLFEEGDLLHDVL
                     ASLNSSELARRRFREIARVSGLISQGFPGQPKSTRQLQASSTLFYEVFRKYDAGNRLL
                     GQAESEVLGQELDIGRLRQVLRRMREQRVVFVELAVPSPFALPLMVERFREKLTTEKL
                     KDRLERMLREMEREAASHDRS"
     gene            3368..3748
                     /locus_tag="VFF72_03100"
     CDS             3368..3748
                     /locus_tag="VFF72_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92174.1"
                     /translation="MNTIVSAFDNRAAAHAAADRLIADGFAADTVQLHDHASAVRNVA
                     ALETDELASGGFFTNLLGLFEQLLGTAPPPGDAGSYAEMVRREGTLLTLHVTVAADAQ
                     RARDLLHAAGAKIVSSLPQVGLEA"
BASE COUNT          536 a         1323 c         1342 g          577 t
ORIGIN      
        1 ctcgtcctct acctgcgccg cgcgcacaag gaggcggtcg gcgccgacaa tttcctcgcc
       61 ctcggcctgg tcgggctggc cttcggcgcc gccagcctcg cctcggcgaa caccttcctg
      121 gccgtcttcg ccgccggctt cgcgctgcgt cgcctcgagc tgcagtcgcc gcggccccgg
      181 cggcatcggc gtgaaggcgc gacgcagcgc gaggcggcac tggcgaaggc cgacgcctcg
      241 gcgaaggcga tcgccgccga agtcgacgat ccgcacgccg ccatcgcccg cgtcggccgg
      301 ctcgccaccg atccgcggca cgcgcccgcc tacatgacgc acgcggtgcg cgagttcaac
      361 gagcagatcc agcgcatcgg cgaggtggcg gcggtcgtcg ccatcggcat gctgctctgg
      421 gcggtcgact ggcacgaggt gtcgtggggc ttcgtcgcgg tgctgctcgt cgtcgtccgg
      481 cccttgtcgg tggcgatcgg cctgttcgcg tcgcgtacct cgctgccgca gcgcggcctc
      541 acggcctggt tcggcatccg cggcgtcggt tcgctgtatt acctggtctt cgcgatccat
      601 caaggcctga ggccgtcgct ggcgtcgacg ctcgcggcgc tggtgctcgg ctcggtcgtc
      661 gtctcgatcg tcgtccatgg catctccgtc acgccgctga tggcgctcta cgaacgccgc
      721 aagcgaagac gcggccgcgc ccgcgacgcc tgatcgtcac cgcggatgcg gccacggcac
      781 cgtcgctgat tccctgtttg cggatccggg cctcggcctg ataccgtggc gccgtgaagg
      841 agaagagcgc gcccggcgag gtctggttcg ccggtcgcgg ctggcagccg ttcgccttcc
      901 agcgcgaagt ctggaaagcg atgcgggccg gccgcagcgg cctcctgcac gcgaccaccg
      961 gctcgggcaa gacgtacgcg gtctggttcg gcgcgatcga gcgcgccgtc gcgctcgacg
     1021 tgccgctgcg cggtgcccat gcgccgtcgc tcggcgtgct ctggctgacg ccgatgcgcg
     1081 ccctcgcggc cgattcggcg cgggcgatcc gagcgtcgat cgacgacctg ggcctgtcgt
     1141 ggacggtggg catccgaacc ggcgacaccg cgtcggccga gcgcgcgaag caggatcgcc
     1201 gcatgccgac ggcgctcgtc acgacgcccg agtcgctgtc gctgatgctg acgcgcgagt
     1261 cggccaggga cgacctctcg ggcgtgcata ccgtcatcgt cgacgagtgg cacgagctga
     1321 tgggcagcaa gcgcggcgtg caggtgcaac tggcgatcgc acgcttgcgg cgctggtgtc
     1381 ccggcctcgt cgtctggggg ctgtcggcga cgctcggcaa tcttcgcgag gcgatgcagg
     1441 tgctgctcgg cgacgacgac ggcgagctcg tgcagggccg gatcgacaag gcgctcgtca
     1501 tcgacacact tcttccggcg acgcccggcc gcttctcgtg gggcggccat ctcggcaagc
     1561 agatgcagca tccggtcgtc gaggagatcg agcgctcgtc gacgaccctg gtcttcgtca
     1621 acatgcgctc gcaggccgag gcgtggtacc agctcatcct cgccgagaag cccgcgtggg
     1681 ccggcctcgt cgcgctgcat cacggctcgc tcgacaagga agtgcgcgac tgggtcgagc
     1741 agggcctcaa gcagggaacg ctgaaggccg tggtggcgac gtcgtcgctc gacctcggcg
     1801 tcgatttcct gccggtcgag cgggtgctgc agatcggctc ggccaagggc gtcgcgcgac
     1861 tgctgcagcg cgccggccgc agcggccacg cgccgggccg ggccagccgc atcacgctgg
     1921 tgccgaccaa cacgctcgag ctgatcgagg ccgtcgcggc gcggcgcgcg gcggcggccg
     1981 gccggatcga gaagcgggcg acccacgaca agccgttcga cgtgctggtg cagcacctcg
     2041 tcacggtcgc gcttggcggc ggcttcaccg ccgacgcgct gttcgacgag gtgcggacgg
     2101 cgtgggcctt tcgcaagctg acacgcgccg agttccagtg ggcgctcgac ttcgtcgtcc
     2161 agggtggcga cagcctgcag gcgtaccccg aataccaccg cgtcgtcgat gtcgagggca
     2221 tctatcgcgt gccgaaccgg gccatcgcgc ggcgccatcg actgggcgtc ggcacgatcg
     2281 tcagcgattc gtcgatgcag gtgaagtacc tgtcgggcgg gcgcatcggc gtcgtcgagg
     2341 agggcttcgt cgcgcgcctg cgcaagggcg actgcttcgt cttcgccggg cgcacgctcg
     2401 agttcgttcg cgccgaggag atgacctgct acgtcaagcg tgccgacggc cgcaaggcgg
     2461 cgatgatcag ctgggccggt gccaagatgc cgctgtcgag cgagcttgcc gactcggtga
     2521 tggaggtgct cgccgatgcc gcccagggcg atttcttcga ccccgagatg gaggccgcgc
     2581 ggcccatgct cgagacgcag atgcagctct cgcggatacc gacgccgaag acgctgctga
     2641 tcgaaaagct cgactctcgc gaaggacagc atctctttct ctatccgttc gccggccgca
     2701 acgcgcacat cggcctgggc agcctgctcg cctggcggct cgccaaggac gcgcccaaca
     2761 cgttttcgat cgtcatcaac gattacggac tcgagctgtt gagcgccagg ccggtcgacc
     2821 cggcgccgct cgtcgaccag agcctcttcg aggagggcga cctgctgcac gacgttctcg
     2881 ccagcctcaa ttcgagcgag ctggcgagac ggcgctttcg cgagatcgcc cgagtcagcg
     2941 gcctgatcag ccagggcttt cccgggcagc cgaagagcac gcgccagctg caggcatcga
     3001 gcaccctgtt ctacgaggtg tttcgcaagt acgacgccgg caatcgactg ctcggacagg
     3061 ccgaaagcga agtgctcgga caggagctcg acatcggccg cctgcgccag gtgctcaggc
     3121 gcatgcgcga gcagcgcgtg gtcttcgtcg agctcgcggt gccgagtccc ttcgcgttgc
     3181 cgctgatggt cgagcggttc cgcgagaagc tcacgaccga gaagctgaag gaccggctcg
     3241 agcggatgct gcgcgagatg gagcgcgagg cggcgagcca cgaccgctcc tgacgcgggc
     3301 gagcgggcac gccgcgtgcc tcgcagcatg gacccgccgc ccgggcgcgg cgcgtcggag
     3361 aaccggcatg aacaccatcg tttccgcatt cgacaaccgg gccgcggcgc acgccgccgc
     3421 cgaccgtctc atcgccgatg gattcgcggc cgacacggtg cagctgcacg accacgccag
     3481 cgccgtccgc aacgtcgccg cgctcgaaac agacgagctg gcgagcggcg gcttcttcac
     3541 caacctgctc ggcctcttcg agcagttgct cggcaccgcg ccgccgccgg gcgacgcggg
     3601 cagctacgcc gagatggtgc gccgcgaggg cacgctgctg acgctgcacg tcaccgtcgc
     3661 cgcggatgcg caacgcgctc gcgacctgct gcacgcggcg ggcgcgaaga tcgtctcgtc
     3721 gctgccgcag gtgggcctgg aagcctgaac gtggcgacgg cagcgaaagg gcccgagg
//
LOCUS       DASRWS010000173         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_926522, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000173 DASRWS010000000
VERSION     DASRWS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926522"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VFF72_03105"
     CDS             complement(<1..1066)
                     /locus_tag="VFF72_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV92175.1"
                     /translation="MNQRIHFIGRLAVSLAAAGAFALPALAATAAKPLQGQVVKLAWI
                     DPLSGPFGPVGTNQLNSWKFFIEKFNANNPAGVKFEITGFDNKVSPAESLNALKLAID
                     QGDRYIIQGNSSGVALALVDAINKYNERNPGKEVVYLNEAAVDPALTNDKCSFWHFRF
                     DADTTMKMEALSSFMKGQTDIHKVYILGQNYSHGIQVAKYAKEDIKRKRPDIQIVGED
                     LHPIGSVRDFSPYIAKIKASGADAVVTGNWGTDLSLLIKAAVEAGYTGKFFTYYAGVT
                     GTPTALGTSDAGKVFVIAYAGGALMGGDYDKDVYAFKKKYNDDFYTGSIIRMLEALSN
                     AMVKANSTDPVKVAFAMEGLK"
     gene            1294..1965
                     /locus_tag="VFF72_03110"
     CDS             1294..1965
                     /locus_tag="VFF72_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HZV92176.1"
                     /translation="MKLLLDFLPIILFFATFKVAEAHADAASRLATDHLGFLVSGGVV
                     GPLEAPVLLATIVVIVATLAQVAFLLARRRKVDTMLWITFVLVTVLGGATVWFHNPTF
                     IKWKPSGLYWAMAVVFWASQAFFGRNLLQQLVGEQLALPAPVWRRLNVAWVAFFLLMG
                     FVNLYVAYHYSTSTWATFKVFGLTGLMAAFMFVQGFYVSRHIKDDDTARGPVSAAAAN
                     TEHGA"
     gene            1962..2228
                     /locus_tag="VFF72_03115"
     CDS             1962..2228
                     /locus_tag="VFF72_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZV92177.1"
                     /translation="MSVAAGDIEQALRAAFSGSTVEVTDDSHLHAGHAGASEGRHFSV
                     RIVSRDFDGLPRVARHRLVYDSLHSLMPRGIHALAIDARVPDPP"
     gene            2295..3116
                     /locus_tag="VFF72_03120"
     CDS             2295..3116
                     /locus_tag="VFF72_03120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZV92178.1"
                     /translation="MKMRSIAASVVLAAVATTGFVPAVALAQNVAVVNGKPVPKSRVD
                     ALMAQVQKQAAARGQQLPPEIEKLARDKVVTDEILSQEAERRGLASTADFKQQMELAR
                     QSILIGLLSADLEKKTTISDADIQKEYDKLKAQSAGTEYHVSHILVQKEDEAKALIAQ
                     LKAGAKFDELAKKNSKDPGSAEKGGDLGFAAPSAYVPEFSQAMVKLKKGEYTQEPVKT
                     QFGYHIIKLDDTREAKFPPLADIKPQIQQRLAQERAMAFRDELRAKAKTDYKFTN"
     gene            complement(3134..3805)
                     /locus_tag="VFF72_03125"
     CDS             complement(3134..3805)
                     /locus_tag="VFF72_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV92179.1"
                     /translation="MSEPIIAVERLTKRVQDSTGVLTILHDIDFTLQAERSVAIVGAS
                     GSGKSTLLAIIAGLDTPSEGTVRLAGVDLFALDEDARAAVRAARLGFVFQSFQLLANL
                     NALENVMLPLELQGRADARLLATEMLKRVGLGERLRHYPRVLSGGEQQRVALARAFVV
                     RPSLLLADEPTGSLDFATGETVMQLMFELNRETGTTLVLVTHDPAIAARCDEQLRIEA
                     GRISV"
BASE COUNT          662 a         1329 c         1265 g          663 t
ORIGIN      
        1 atttcaggcc ttccatggca aaggcgacct tgaccgggtc ggtggagttg gccttgacca
       61 tcgcgttcga cagcgcctcc agcatccgga tgatcgagcc ggtgtagaag tcgtcgttgt
      121 acttcttctt gaaggcgtag acgtccttgt cgtagtcgcc gcccatgagg gcgccgccgg
      181 cataggcgat cacgaagacc ttgccggcat cgctcgtccc gagcgccgtc ggcgtgccgg
      241 tgacgccggc gtagtaggtg aagaacttgc cggtgtagcc ggcctcgacc gcggccttga
      301 tcaggagcga aaggtcggtc ccccagttgc ccgtgacgac cgcgtcggcg cccgaagcct
      361 tgatcttggc gatgtagggc gagaagtcgc gaaccgatcc gatcggatgg aggtcctcgc
      421 cgacgatctg gatgtcgggg cgcttcctct tgatgtcttc cttcgcgtac ttggcgacct
      481 ggatgccgtg cgagtagttc tggccgagga tgtagacctt gtggatgtcc gtctggccct
      541 tcatgaagct ggaaagcgcc tccatcttca tcgtcgtgtc ggcgtcgaag cggaagtgcc
      601 agaagctgca cttgtcgttc gtcagcgcgg gatcgacggc ggcctcgttc aggtagacca
      661 cttccttgcc cgggttgcgc tcgttgtact tgttgatcgc atcgacgagc gccagggcga
      721 cgccggagct gttgccctgg atgatgtagc ggtcgccctg atcgatcgcg agcttcagcg
      781 cgttgagcga ctcggccggc gagaccttgt tgtcgaagcc ggtgatctcg aacttcaccc
      841 cggccgggtt gttcgcgttg aacttctcga tgaagaactt ccagctgttg agctggttgg
      901 tgccgaccgg accgaacggg cccgacaacg ggtcgatcca ggccagcttg acgacctggc
      961 cctgcaaagg cttcgccgca gtggccgcga gcgcaggcag cgcgaaggcg ccggcggcag
     1021 ccaggctcac tgccagcctg ccgatgaaat ggatgcgttg gttcacgtaa gctcctcgga
     1081 cgaaacgacg accgtcgcgg aaggtaacgg aaggaacacc ggcgcggcaa cagggactac
     1141 ccccaaacgc cgcgtcccgg cagcgacacc ggccgtgccc gaagggcttt gcgcagggcg
     1201 cccgaccggg ctgactacac tgcacgccat gcaagtcacc gcgaatcctt cccgccaggc
     1261 tcccgccgac cgagcgacga aaccacgccg ccgatgaagc tgctgctcga cttcctgccg
     1321 atcatcctct tcttcgccac cttcaaggtg gccgaagccc acgccgacgc cgcgtcgcgg
     1381 ctggccacgg atcacctggg gttcctggtc tcgggcggcg tcgtcgggcc cctggaagcg
     1441 ccggtcctgc tggccaccat cgtcgtcatc gtcgcgacgc tggctcaggt cgcgttcctg
     1501 ctggcccggc gtcgcaaggt cgacaccatg ctctggatca cgttcgtcct cgtcaccgtg
     1561 ctgggcggag cgacggtatg gttccataac cccaccttca tcaagtggaa gccgagcggg
     1621 ctctactggg cgatggccgt ggtgttctgg gccagccagg cgttcttcgg ccgcaacctg
     1681 ctgcagcaac tggtgggcga gcagctcgcc cttccggcgc ccgtctggcg ccgcctcaac
     1741 gtcgcctggg tcgccttctt cctgctgatg ggcttcgtca acctctacgt ggcctaccac
     1801 tactcgacat cgacctgggc gaccttcaag gtcttcggcc tgaccggcct catggcggcg
     1861 ttcatgttcg tgcaggggtt ctacgtgtcg cggcacatca aggacgacga caccgcgcgc
     1921 gggcccgtca gcgctgccgc cgccaacacg gagcacggcg cttgagcgtt gccgccggcg
     1981 acatcgagca ggcgctgcgc gccgccttct ccggctcgac cgtcgaggtc accgacgaca
     2041 gccacctgca cgcgggccat gccggggcca gcgagggccg gcacttcagc gtccgcatcg
     2101 tcagccggga tttcgatggc cttccccgcg tcgcgcgtca tcgcctcgta tatgattccc
     2161 tgcactcact catgccgcgt gggatccacg ccttggccat cgacgcccgc gttccggacc
     2221 cgccctgaat cctcgggccg ggccgtgaag atgaggcgac ggccgtcctc aacccgcccg
     2281 aaaggccccg ttccatgaag atgcgttcca tcgccgccag cgtcgtgctg gctgccgtcg
     2341 cgaccacggg cttcgtcccg gcagtcgccc ttgcccagaa tgtcgccgtg gtcaacggca
     2401 agccggtgcc gaagtcgcgc gtcgacgcgc tgatggccca ggtccagaag caggccgcgg
     2461 cccgtggcca gcaactgccg ccggaaatcg agaagctcgc ccgcgacaag gtcgtcaccg
     2521 acgaaatcct ctcgcaggaa gccgaacggc gcggcctggc gtcgaccgcc gacttcaagc
     2581 agcagatgga gctcgcgcgt caaagcatcc tgatcggcct tctctccgcc gacctggaaa
     2641 agaagacgac gatcagcgac gccgacatcc agaaggaata cgacaagctg aaggcgcaat
     2701 cggctggcac cgagtaccac gtcagccaca tcctggtgca gaaggaagac gaagcgaagg
     2761 cgctgatcgc ccagctcaag gcgggcgcca agttcgacga gctggcgaag aagaactcca
     2821 aggatccggg ctcggcggaa aaaggcggcg acctcggctt cgccgcgccc agcgcctacg
     2881 tgcccgagtt ttcccaggcg atggtcaagc tgaagaaagg cgagtacacg caggagccgg
     2941 tgaagacgca gttcggctac cacatcatca agctcgacga cacgcgcgaa gccaagttcc
     3001 cccccctggc cgacatcaag ccccagatcc agcagcggct cgcccaggaa cgtgccatgg
     3061 ccttccgcga cgagcttcgc gccaaggcga agaccgacta caagttcacc aactgatccc
     3121 ggccgcgggc cggtcagacg ctgatccggc ccgcctcgat gcgcagttgc tcgtcgcaac
     3181 gcgcggcgat cgccggatcg tgcgtgacga ggaccagggt cgtcccggtc tcgcggttca
     3241 gctcgaacat cagctgcatc accgtctcgc cggtcgcgaa gtcgaggctg ccggtcggct
     3301 cgtcggcgag cagcaggctc ggccggacga cgaaggcacg ggccagggcg acgcgttgct
     3361 gctcgcctcc cgagagcacg cgcggatagt ggcgcaggcg ttcgccgagg ccgacccgct
     3421 tcagcatctc cgtcgccagc aggcgcgcat cggcacgtcc ctgcagctcg agcggaagca
     3481 tcacgttctc gagggcgttc aggttggcga gcaactggaa gctctggaac acgaagccca
     3541 gtcgtgccgc ccgcaccgcg gcgcgtgcat cctcgtcgag cgcgaagagg tcgacgccgg
     3601 ccaggcgcac cgtgccctcg ctcggcgtat cgagcccggc gatgatcgcg agcaaggtgc
     3661 tcttgcccga gcccgaggcg ccgacgatgg ccaccgaacg ctcggcctgc agggtgaaat
     3721 cgatgtcgtg gagaatcgtc aggacgccgg tcgagtcctg cacgcgcttg gtcagccgct
     3781 cgacggcgat gatgggctcc gacatggatg gcgcggctga ttcttctggt ttgactgcac
     3841 cggcacgacg ccgctggctg gcgcacttta gcgtggccct tgcattcgtc tcggcggtcg
     3901 cctcgatgcc ctcgctcgg
//
LOCUS       DASRWS010000174         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3518049, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000174 DASRWS010000000
VERSION     DASRWS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518049"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFF72_03130"
     CDS             complement(<1..330)
                     /locus_tag="VFF72_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZV92180.1"
                     /translation="MDEGRGVIASTTHLVVIPSYNTGATVYETVRAARAAWAPVWVVV
                     DGSDDGTAEGLRALAATDPALRVDVLPANRGKGAAVLQALETAAGAGFTHALTMDSDG
                     QHPASLIA"
     gene            complement(356..1105)
                     /locus_tag="VFF72_03135"
     CDS             complement(356..1105)
                     /locus_tag="VFF72_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZV92181.1"
                     /translation="MTTLDATKTPGALQRLWFAILIAQLGLMSLGWNLLAPLLSLLLP
                     RRLGTRVGRRAILVIYRCLWASATRLGMMRIDAGALDALDDEPGGLIVAANHPTMLDA
                     LVIVARLPRGVCIMKAELLRNIFLGAGARLARYIRNDAGRGMVRDAVASLKEGGQLVF
                     FPEGTRTVRPPVNEFKPGVALISHLAGVPIQTVLIESDSPYLTKGWPLLRPPPIPVVI
                     RVRLGDRFAPEADHRALRRRLEAYFHRELQR"
     gene            complement(1102..2028)
                     /locus_tag="VFF72_03140"
     CDS             complement(1102..2028)
                     /locus_tag="VFF72_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZV92182.1"
                     /translation="MTRVLLVRGRHAAPAILLAGLVLAAATAARADDVPVLGPVVQGS
                     VEKQGGADSSSRGEPLWELGMGVAAVRFADYRGSDHTSSYVLPLPFVAYRGRFLRADR
                     DGARAILFAGHQVVVDLSLSASVPTRSDRDDARRGMPDLAGTFEIGPNLNVELWQSAD
                     HRYKLDLRLPVREAITLQSSPRTIGVTFSPNLNLDVRNFAGSWNLGMLAGPLYADRRY
                     HGYFYSVAPEFATESRPAYKAPGGYAGWRATTAFSRRIGNAWLGGFLRYDDLNRAAFV
                     ASPLVRRQNSLTAGIGVSWIFAVSGQRVVVDD"
     gene            complement(2025..3365)
                     /locus_tag="VFF72_03145"
     CDS             complement(2025..3365)
                     /locus_tag="VFF72_03145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZV92183.1"
                     /translation="MLTESVPAPDQPPQPLPAFDCDVLVVGGGPAGSTIAALLAERGR
                     DVVLLEKGRHPRFHIGESLLPANAELFDALGVRGEVDRLGMAKYGVEFVSPDHDRSAM
                     IEFVEAWDKSMSYAWQVRRSELDELLFRNAAAKGAKTIEGSRVRQVRFDAHGASVDAE
                     FEGVARTWRTRFVVDASGRDTLLANQLRSKQKNRKHNSSALFAHFEGATRLPGKSEGN
                     ITIFWFEHGWFWFIPLADGVTSIGAVCWPYYLKTRTRPLPDFFADTIASCPALAERLA
                     GATRISDVQATGNYSYSSTVCSGERFVLLGDAFAFIDPVFSSGVLLAMKSAFAGADVV
                     ETTLDRPREAARARRRFDALSRHGTREFSWFIFRVSSPTLRGFFMQPQNPLRVKEALI
                     SLFAGDIYGKTPIWPSIYALKALYYIVSLGNLGRSLAFWRRRRLNIRDVEVPAS"
     gene            complement(3519..4223)
                     /locus_tag="VFF72_03150"
     CDS             complement(3519..4223)
                     /locus_tag="VFF72_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92184.1"
                     /translation="MLRVKGLRVAYGGIQAVKGVSFEVEEGELVSLIGANGAGKTTTL
                     KAITGTLPSTGGEIEFLGRSIAGQGPWDLVRQGLVMVPEGRGVFSRMSITENLQMGAF
                     VRDDKAGIEADIERIFGIFPRLKERRNQLAGTMSGGEQQMLAMGRALMARPKVLLMDE
                     PSMGLSPLLVDKIFEVVDDIHRRGTTILLVEQNASRALQLASRGYVMDSGEVTMHGPA
                     KELLADPKVRAAYLGQ"
BASE COUNT          712 a         1424 c         1510 g          602 t
ORIGIN      
        1 agcgatcagc gaagccggat gctggccgtc ggagtccatc gtcagcgcgt gcgtgaagcc
       61 ggcgccggcg gcggtttcca gcgcctgcag cacggccgcg cccttgccgc gattggcagg
      121 gagcacgtcg acgcgcagcg ccggatcggt cgcggcgagg gcacgcaagc cttcggcggt
      181 gccgtcgtcg ctgccgtcga cgacgaccca gaccggcgcc caggcggcgc gtgccgcgcg
      241 aaccgtctcg tacacggtgg cgccggtgtt gtagctcggg atgacgacga ggtgcgtcgt
      301 cgacgcgatc acgccgcgtc cctcgtccat acgcgacacc tgagcggcac ctgcctcacc
      361 gctgcagctc gcggtggaag tacgcttcga ggcggcggcg cagtgcgcga tggtcggcct
      421 cgggcgcgaa gcggtctccc agccggacgc ggatcaccac cggaatgggc ggcggccgaa
      481 gcagcggcca gcccttggtg aggtacggtg agtcgctctc gatcaaaacg gtctggatcg
      541 gcacgccggc aaggtgggag atcagggcga cgccgggctt gaactcgttc accggcggcc
      601 gcacggtgcg ggtgccttcg gggaagaaga ccagctggcc gccctccttc agcgaggcca
      661 ccgcatcccg caccatgccg cgtcctgcgt cgttgcggat gtagcgagcc agccgcgcgc
      721 cggcgcccag gaaaatgttg cgcagcaact cggctttcat gatgcagacg ccgcgaggca
      781 ggcgggcgac gatgacgagc gcatcgagca tggtcggatg gttcgccgcg acgatgagac
      841 caccgggctc gtcgtccagc gcgtcgagag cgccggcatc gattcgcatc atgcccagcc
      901 gcgtcgccga cgcccagagg cagcggtaga tgacgaggat ggcccggcga ccgacgcgcg
      961 tgccgagccg gcggggcagc agcagcgaca gcagcggcgc gagaagattc cagccgagcg
     1021 acatcaggcc aagctgcgcg atcaggatcg cgaaccacag gcgctgcagt gcgccgggtg
     1081 tcttcgtggc gtcgagcgtc gtcaatcgtc gaccaccact cgctgacccg acacggcgaa
     1141 gatccaggaa acgccgatgc cggcagtcaa cgagttctgg cggcggacca gcgggctcgc
     1201 gacgaaggcg gcacgattca ggtcgtcgta gcggagaaag ccgcccagcc aggcgttgcc
     1261 gatccggcgc gagaaggccg tcgtcgcacg ccagcctgcg tagccaccgg gcgccttgta
     1321 ggccggccgc gactcggtcg cgaattcggg agcgacgctg tagaagtacc cgtggtagcg
     1381 acgatcggca tagagcgggc cggcgagcat gccgaggttc cagctgccgg cgaagttgcg
     1441 cacgtcgagg ttgagattgg gcgaaaaggt gacgccgatc gttcgcggcg acgactgcag
     1501 cgtgatcgcc tcgcgcaccg gcagtcgcag gtcgagcttg tagcggtgat cggccgattg
     1561 ccacagctcg acgttgaggt tggggccgat ctcgaaggtg ccggcgagat cgggcatgcc
     1621 acggcgggca tcgtcgcgat cgctgcgcgt cggcaccgaa gccgagaggc tcaggtcgac
     1681 gacgacctgg tggccggcga agagaatcgc ccgcgcgccg tcgcggtcgg cccgcaggaa
     1741 gcggccgcga taggcgacga agggcagcgg cagcacgtag ctgctggtgt gatcggagcc
     1801 gcgatagtcg gcgaaccgca ccgcggcgac gcccatgccc agctcccaaa ggggctcgcc
     1861 ccgggacgac gagtcggcgc cgccctgctt ctcgacgctg ccctgcacga ccggcccgag
     1921 caccggcacg tcgtcggcgc gcgcggccgt cgccgctgcg agaacgaggc ccgcgagcag
     1981 gatcgcgggc gccgcatgcc ggccccgcac gagaagcact cgcgtcacga cgcgggaacc
     2041 tcgacgtcgc ggatattcag gcgacgccgg cgccagaagg cgaggcttcg ccccaggttg
     2101 ccgagcgaga cgatgtaata gagcgccttc agggcataga tcgacggcca gatcggtgtc
     2161 ttgccgtaga tgtcgcccgc gaagagcgag atcagcgctt ccttgacgcg cagcgggttt
     2221 tgcggctgca tgaagaatcc gcgcaatgtc gggctcgaga cacgaaagat gaaccacgag
     2281 aactcgcgcg tgccgtggcg cgacaacgcg tcgaagcggc gtcgggcccg cgccgcctcg
     2341 cgcggccgat cgagcgtcgt ctcgacgacg tcggcgccgg cgaaggcgct cttcatcgcc
     2401 agcagcactc ccgaagaaaa caccggatcg atgaatgcga aggcatcgcc cagcaggacg
     2461 aagcgctcgc cgctgcagac cgtgctgctg tacgagtagt tgccggttgc ctggacgtcg
     2521 gaaatgcgcg tcgccccggc gagccgttcc gcgagtgcgg ggcacgaggc gatcgtgtcg
     2581 gcgaagaaat cgggcagcgg ccgggtccgg gtcttcaggt agtaaggcca gcagacggcg
     2641 ccgatgctgg tgacaccgtc ggccagcggg atgaaccaga accagccgtg ctcgaaccag
     2701 aagatggtga tgttgccttc ggactttccg ggcaggcgcg tcgcgccttc gaagtgggcg
     2761 aacagcgccg agctgttgtg cttgcggttc ttctgcttgc tgcgcagctg gttcgcgagc
     2821 agcgtgtcgc ggccggacgc gtcgacgacg aagcgcgtcc gccacgtccg ggcgacgcct
     2881 tcgaactcgg cgtcgacgct cgcgccatgc gcgtcgaagc ggacctgcct cacccggctg
     2941 ccttcgatgg tcttcgctcc cttggccgcc gcgttgcgaa agagcagttc gtcgagctcg
     3001 gagcggcgca cctgccaggc gtacgacatc gacttgtccc acgcttcgac gaactcgatc
     3061 atggccgacc ggtcgtgatc gggcgaaacg aattcgacgc cgtatttcgc catgcccagg
     3121 cggtcgacct cgccgcgcac gccgagcgca tcgaagagct cggcgttggc aggcaacagc
     3181 gactcgccga tgtgaaagcg cgggtggcgg cctttctcga ggagaaccac gtcgcgcccg
     3241 cgctcggcga ggagcgccgc gatcgtcgag ccggccgggc cgccgccgac gacgaggacg
     3301 tcgcaatcga aggccggcag cggctgcggc ggctggtcag gggcgggaac ggactcggtc
     3361 agcatcgaaa ctccgaagcg cccggcggag cctcgcgccc cttcgcacaa gcccccgagc
     3421 cttgcccgga ttctatcgtc ggcgcccgtc gcagccgacg ccgaccgggg ccgccggcac
     3481 gacggatacc ggcccttcgt gacggacccg ccaaacgact actggccgag gtaggcggcg
     3541 cgcaccttgg ggtcggcgag aagctccttg gcagggccgt gcatcgtcac ttcgccggag
     3601 tccatgacgt agccgcgcga tgccagctgc agcgcgcggc tggcgttctg ctcgacgagc
     3661 aggatcgtcg tgccgcggcg gtggatgtcg tcgacgacct cgaagatctt gtcgacgaga
     3721 aggggcgaaa gccccatcga gggttcgtcc atcagcagga ctttcggacg cgccatcagc
     3781 gcccggccca tcgccagcat ctgctgctcg ccaccgctca tcgtgccggc gagctggttg
     3841 cgacgctcct tcaggcgcgg aaagatgccg aagatgcgct cgatgtcggc ctcgatgccg
     3901 gccttgtcgt cgcgcacgaa cgcacccatc tgcaggttct cggtgatcga catgcgcgag
     3961 aacacgcccc gtccctcagg caccatgacg aggccctgcc ggacgagatc ccagggccct
     4021 tggccggcga tgctgcgacc gaggaactcg atctcgccgc cggtcgaagg aagcgttccg
     4081 gtgatcgcct tcagcgtcgt cgtcttgccg gcgccgttgg cgccgatgag gctgacgagc
     4141 tcgccctcct cgacctcgaa cgaaacgccc ttgaccgcct ggatgccgcc gtaggcgacg
     4201 cgcaggccct tcactcgaag cagcgtcttg gtgctgcccg gcgcgggc
//
LOCUS       DASRWS010000175         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_927808, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000175 DASRWS010000000
VERSION     DASRWS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927808"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFF72_03155"
     CDS             <1..702
                     /locus_tag="VFF72_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92185.1"
                     /translation="MGARAADTQLRTRAVVVVRSAQQRVAVHVDEVLGNQEVVVKNLG
                     PQLSRLPGLAGMTLLASGAVALIYNPVALATLYGERARVATQAAMRPDSGAPGTPAAG
                     AADAPRAPLVLVVDDSLTVRRVTQRMLLREGYRVTLAKDGLDALERLAEETPQVILSD
                     IEMPRMDGFDLVRNLRADARWRGLPVIMITSRIAEKHRDHAAELGVDHYLGKPYSEED
                     LLALVARYTSAVAEA"
     gene            complement(709..2196)
                     /locus_tag="VFF72_03160"
     CDS             complement(709..2196)
                     /locus_tag="VFF72_03160"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZV92186.1"
                     /translation="MDKALDTALVWLRRDLRCDDNAALFHALKAARQVWCVFVYDREI
                     LDPLPRADRRVEFIVECVDALDASLAALGRSRDVHGVRLIVRHGIAVDEIVGLAGELH
                     VQAVYANHDDDPAALARDAHVRGALADRGIALHTSKDHVIFERNEVLTGSGKAYSVFT
                     PYRTAWLKKLDEFYVKAYPVERHAASLAPVPAGIAVALPSLAELGFIRTNLAALKVEG
                     GESSARALLGDFLGRIDEYDTAREFPAVKGPSYLGVHLRFGTISIRELVRAALGRGGR
                     PASRGAEVWLSELVWRDFYQQVLHHFPHVVGHACKPEFERLRFEHGKHADALFTAWCE
                     GKTGYPLVDAAMAQINQTGYMHNRLRMVAASFLVKDLGIDWRLGEAYFATHLIDYELA
                     SNNGGWQWAASTGFDAQPWFRIFNPLTQSRKFDPGGRFIRRYLPQLARLDDVVIHAPW
                     LAGPVDLEAAGIVIGRDYPAPLVDHAAARERTLARYAAARKKPPP"
     gene            2274..2861
                     /locus_tag="VFF72_03165"
     CDS             2274..2861
                     /locus_tag="VFF72_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZV92187.1"
                     /translation="MSAAAINLTNQFLIAMPGTGGGAFAGSVVYMCEHTDKGALGLVI
                     NKPIDITLKNLFEKVELSLDREDLAATPVYFGGPVQTERGFVLHEPTGSGGEEESGYN
                     SSLKIEGGLEMTTSKDVLEALASGAGPKRVLVTLGYSGWAAGQLEEEMGRNSWINVAA
                     EPGIIFDTPVAERYGKALLLLGIDAGMLSTAAGHA"
     gene            2935..3348
                     /gene="ruvX"
                     /locus_tag="VFF72_03170"
     CDS             2935..3348
                     /gene="ruvX"
                     /locus_tag="VFF72_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZV92188.1"
                     /translation="MRPALAPSASQVRSFLGFDFGTRRVGVATGNTLTRTASPLTTIA
                     AEGKARIDSIAALVEAWQPAALVVGVPLHPDGAAHVNTGRARRFARQLRGRFPLPVHE
                     VDERYTTVEARARGASDLDAAAAVVILEQYLADMP"
     gene            3345..3842
                     /gene="pyrR"
                     /locus_tag="VFF72_03175"
     CDS             3345..3842
                     /gene="pyrR"
                     /locus_tag="VFF72_03175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZV92189.1"
                     /translation="MSDVASLDAEALYASLVEGVRPLLRPQGALVGIWSGGAWLAERL
                     QRDLGLPGDAGVISSTLHRDDFSRRGLAGGADATRLPFEIEGCHIVLVDDVLYTGRTI
                     RAVVNELYDFGRPASVRLAVLVDRGGRELPIEASFAAATTEVAAERRLSLARDAAGRF
                     SFEIR"
     gene            3972..>4376
                     /gene="pyrB"
                     /locus_tag="VFF72_03180"
     CDS             3972..>4376
                     /gene="pyrB"
                     /locus_tag="VFF72_03180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZV92190.1"
                     /translation="MFVRRNPQLNRNDELIHLLSIEGLPASVVHHILDTAGTFLSVND
                     REVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDIARSSTAKGESLLD
                     TIANLSAMHADMFVVRHSESGAPYLIARHCAPH"
BASE COUNT          668 a         1530 c         1518 g          660 t
ORIGIN      
        1 atgggcgcgc gcgccgccga cacgcagctg cgcacccggg cggttgtcgt cgtgcgcagc
       61 gcccagcagc gggtcgccgt gcacgtcgac gaagtcctcg gcaaccagga agtcgtcgtc
      121 aagaacctcg gtccgcagct gtcgcgcctg cccggactcg ccggcatgac gctgctggcc
      181 tcgggcgcgg tggccctgat ctacaacccg gtcgccctgg ccacccttta cggcgagcgg
      241 gcgcgggtgg cgacccaggc cgccatgcgt cccgattcgg gcgcccccgg cacgccggcc
      301 gccggcgcgg cggatgcgcc gcgggcaccg ctggtcctcg tcgtcgacga ttcgctcacg
      361 gtgcgccgcg tcacccagcg gatgctgctg cgcgaggggt atcgcgtgac gctggccaag
      421 gacggcctcg acgcgctcga gcgcctggcc gaggaaacgc cgcaggtcat cctctcggac
      481 atcgagatgc cgcgcatgga cggcttcgat ctcgtccgca acctgcgagc cgacgcgcgc
      541 tggcggggcc tgccggtgat catgatcacg tcgcgcatcg ccgagaagca ccgcgaccac
      601 gccgccgagc tcggcgtcga tcactacctc ggcaagccgt actccgaaga agacctgctg
      661 gccctcgtcg cccgctacac gagcgccgtc gccgaagcct gatcggcgtc acggcggcgg
      721 cttcttcctg gcggcggcgt agcgggcgag cgttcgctcg cgggcggcgg cgtgatcgac
      781 gaggggcgcc gggtagtcgc ggccgatgac gatgccggca gcttccagat cgaccggtcc
      841 ggccagccac ggcgcgtgga tgacgacgtc gtcgagccgc gccagctgcg gcaggtagcg
      901 acggatgaag cgcccgccgg ggtcgaactt gcggctctgg gtcagcgggt tgaagatccg
      961 gaaccacggc tgcgcgtcga agccggtcga cgccgcccac tgccagccgc cgttgttcga
     1021 ggcgagctcg tagtcgatca ggtgggtggc gaaatacgcc tcgccaagcc gccagtcgat
     1081 gcccagatcc ttgaccagaa agctcgcggc taccatgcgc aggcggttgt gcatgtagcc
     1141 ggtctggttg atctgcgcca tcgccgcgtc gacgagcgga tagcccgtct ttccctcgca
     1201 ccaggccgtg aagagcgcgt cggcgtgctt gccgtgctcg aagcgaagtc gctcgaattc
     1261 gggcttgcag gcatggccca cgacatgcgg gaagtggtgc agcacctgct ggtagaagtc
     1321 gcgccagacc agctcggaca accagacctc ggcgcctcgg ctcgcaggac ggccgccgcg
     1381 tccgagcgcg gcgcgtacca gctcccggat cgagatggtg ccgaagcgca ggtgcacgcc
     1441 gaggtagctc ggccctttca ccgccggaaa ctcgcgggcc gtgtcgtatt cgtcgatgcg
     1501 accgaggaag tcgccgagaa gtgcccgtgc ggacgattcg ccgccttcga ccttcaacgc
     1561 cgcgagattg gtgcggatga atccgagctc ggccagcgac ggcagcgcga cggcgatgcc
     1621 tgccggtacg ggagcgaggc tcgccgcatg acgctcgacc gggtaggcct tgacgtagaa
     1681 ctcgtcgagc ttcttcagcc aggccgtgcg atagggcgtg aagacggaat acgccttgcc
     1741 gctccccgtc aacacttcgt tccgctcgaa gatgacgtgg tccttcgagg tgtgcagggc
     1801 gatgccgcga tcggccagcg cgccgcggac gtgggcatcg cgggccagcg ccgccgggtc
     1861 gtcgtcgtga ttcgcgtaga cggcctggac gtgcagctcg ccggcgaggc cgacgatctc
     1921 gtcgacggcg atgccgtgcc gcacgatcag ccggacgccg tggacgtcgc ggctgcggcc
     1981 gagcgcggcc agcgatgcgt cgagcgcgtc gacgcattcg acgatgaact cgacgcggcg
     2041 atcggcacgc ggcagcggat cgaggatctc gcggtcgtag acgaagacgc accacacctg
     2101 gcgtgccgcc ttgagggcat ggaacagcgc cgcgttgtcg tcgcagcgca ggtcgcggcg
     2161 aagccatacc aacgccgtgt cgagggcctt gtccatgggc cgccagtgtg cccaaaagcc
     2221 cggcgaaggc ctcggggtcg cacgggccgc gcggcgcgga ataaaatcgg cccatgtcgg
     2281 ctgcggccat caacctgacg aaccagttcc tgattgccat gcccggcacg ggcggcggcg
     2341 ccttcgcagg cagcgtcgtc tacatgtgcg agcacaccga caagggtgcg ctcggcctgg
     2401 tcatcaacaa gcccatcgac atcaccctga agaacctgtt cgagaaggtc gaactgtcgc
     2461 tcgaccgcga ggacctcgcc gccacgccgg tgtatttcgg cggtccggtg cagaccgagc
     2521 gcggcttcgt tctgcacgag ccgacgggct cgggcggcga ggaagaatcc ggctacaact
     2581 cgtcgctgaa gatcgagggc ggcctcgaga tgacgacgag caaggacgtg ctggaggcgc
     2641 ttgccagcgg cgcggggccg aagagggtgc tcgtgacgct cggctacagc ggctgggcgg
     2701 ccggccagct cgaggaagaa atgggccgca acagctggat caacgtcgcc gccgagcccg
     2761 gcatcatctt cgacacgccg gtcgcggagc gatacggcaa ggcgctcctg cttctgggca
     2821 tcgacgccgg catgctcagc acggcggcgg gccacgcatg aacgcgtcgc cgccgctgcc
     2881 gggttcgcgt cgccccgccg ctcgggcggc ccggcagtgc gccggagtgt ctcggtgagg
     2941 ccggccctgg cgccatccgc ctcgcaggtc cgctcctttc tcggtttcga tttcggcacc
     3001 cgccgtgtcg gtgtcgccac cggcaacacg ctgacacgga ccgcatcgcc gctcacgacc
     3061 attgccgccg aaggcaaggc acggatcgac tcgatcgccg cgctggtgga ggcctggcaa
     3121 ccggccgcgc tcgtcgtcgg cgtgcccctg catccggacg gcgccgccca cgtcaatacc
     3181 ggtcgggctc gccgtttcgc acggcagctg cgcgggcgct ttccgctgcc cgtgcacgag
     3241 gtcgacgagc gctacacgac ggtcgaggcg cgcgcccgcg gcgccagcga cctcgacgcc
     3301 gcggcggccg tggtcatcct cgagcaatac ctggcggaca tgccatgagc gacgtggcct
     3361 cgctcgacgc ggaggcgctc tacgcctcgc tggtcgaagg tgtccgcccg ctgctgcggc
     3421 cgcagggcgc gctggtcgga atctggtcgg gcggcgcctg gctggccgag cgcctgcagc
     3481 gcgacctcgg cctccccggc gacgcgggtg tcatctcgag cacgctgcat cgcgacgatt
     3541 tctcgcgccg cggactggcc ggcggcgccg acgcgacgcg gctgccgttc gagatcgaag
     3601 gttgtcatat cgtcctcgtc gacgacgtgc tctacaccgg ccggacgatc cgagcggtgg
     3661 tcaacgagct ctacgatttc ggaaggccgg cgagcgtgcg gctggccgtg ctcgtcgatc
     3721 gcggtggacg cgagctgccg atcgaggcgt cgttcgctgc ggccacgacc gaggtcgctg
     3781 ccgagcgccg gctttcgctg gcccgcgacg ccgccggccg cttcagcttc gagatcaggt
     3841 agcgtggatc gctgtcctgc cgacacccat cgccgcttcg acgcggacgc gcccgctgcc
     3901 ggcgggcgag ggcggcagcc cggtccgggg ccgtgcacgt gccggcccaa ccccgacgtc
     3961 gacgactgat catgttcgtt cgccgcaatc cgcagctcaa ccgcaatgac gagctcatcc
     4021 atctgttgtc gatcgaaggc ctgccggcgt cggtcgtgca ccacatcctc gacaccgccg
     4081 gaacctttct cagcgtcaac gaccgcgagg tgaagaaggt gccgctgttg cgcggcaaga
     4141 gcgtcttcaa cctcttcttc gagaactcga cccgcacgcg caccaccttc gagatcgccg
     4201 ccaagcggct gtcggccgat gtcattaacc tcgatatcgc gcgatcgtcg accgccaagg
     4261 gcgaaagcct tctcgacacc atcgccaacc tctcggcgat gcatgccgac atgttcgtgg
     4321 tccgccattc ggaatctgga gcgccctacc tgatcgcgcg gcattgcgcg ccgcac
//
LOCUS       DASRWS010000176         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_71930, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000176 DASRWS010000000
VERSION     DASRWS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71930"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFF72_03185"
     CDS             complement(<1..665)
                     /locus_tag="VFF72_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZV92191.1"
                     /translation="MTTTPVPTPARLRLFSPAGVLRSAVPLRRAVARLGKLGFAVEVD
                     EAATARAQRFAGDDADRLAALHRVAEAKPDVALATRGGYGLTRLLDRIDWKRIARSVD
                     SGTRWVGMSDLTALQLGLLEHTGATSWAGPLACDDFGRSDAEGGVDEVTQGCFTEAMS
                     GVLEAIGFRTGEGFDGLSVRGILWGGNLCVVNSLLATAHFPDVKGGILFLEDVNEHPY
                     RVER"
     gene            complement(662..1291)
                     /gene="tadA"
                     /locus_tag="VFF72_03190"
     CDS             complement(662..1291)
                     /gene="tadA"
                     /locus_tag="VFF72_03190"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZV92192.1"
                     /translation="MPHDDEAGMRLAIDQAHNAWLVGEVPVGAVIVRDSPSGRQVVAT
                     GYNRPITDHDPTAHAEIVALRHAAQLLGNYRLPECEIYVTLEPCAMCAMALLHARVKR
                     VVYAATDPKAGAAGSVIDLFANTALNHHTAVTGGVLADASAQLLRSFFAERREQYRQR
                     RGATTLPAPLAAGAGAPALPGGDDSLAMAADVSPPSIPAGESVELDGDA"
     gene            complement(1291..1647)
                     /locus_tag="VFF72_03195"
     CDS             complement(1291..1647)
                     /locus_tag="VFF72_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92193.1"
                     /translation="MMKAIFGILGLVLVLAIVGSIAKKQLQAVGLAGGGPGNVVTRAD
                     EASRRAADAAADTVPAGDRDGATLAIPGGMPGAVAADPNIGTVPQQARNIESQVRDNA
                     VRAMQQGMQRNQRAEP"
     gene            complement(1717..1968)
                     /locus_tag="VFF72_03200"
     CDS             complement(1717..1968)
                     /locus_tag="VFF72_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92194.1"
                     /translation="MRIFEGFVFLAIGVGLIAMTLRSLGAGWLPCGSRGFAERLEFRR
                     DEEPLGYWAMFVVYGAAGVGAIVFGLRLLTGHATPLPLH"
     gene            complement(1993..>2361)
                     /locus_tag="VFF72_03205"
     CDS             complement(1993..>2361)
                     /locus_tag="VFF72_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92195.1"
                     /translation="RRRSCVRTSGGDFFMALVVSMIVGLLSMPSATVFALARYLRHRR
                     AVPDDRRVTIGAFVGIGLLVAFGLYFAAATLSIDHACSRPRAGNLCGMIGAVVIGPIV
                     ASLGIVIVSVIGLPVAARRR"
BASE COUNT          375 a          827 c          848 g          311 t
ORIGIN      
        1 cgctcgaccc ggtagggatg ctcgttgacg tcctcgagaa agaggatgcc gcccttgacg
       61 tcaggaaaat gcgccgtcgc cagcagcgag ttgacgacgc agagatttcc tccccagagg
      121 atgccgcgca cgctgagacc gtcgaaaccc tcgccggtgc gaaagccgat cgcctcgagc
      181 acgcccgaca tcgcctcggt gaagcagccc tgcgtcacct cgtcgacgcc gccttcggcg
      241 tcgctgcggc cgaagtcgtc gcaggccagc ggccccgccc agctcgtcgc gccggtgtgc
      301 tcgagaaggc cgagttgcag cgcggtgagg tcgctcatgc cgacccagcg cgttccgctg
      361 tcgacgctgc gggcgatccg cttccagtcg atgcggtcga gcagccgcgt caggccgtag
      421 ccgccgcgcg tcgccagggc gacatcgggc ttggcttcgg cgacgcgatg cagcgccgcc
      481 agccggtccg cgtcgtcgcc ggcaaagcgc tgcgccctgg ccgtcgccgc ctcgtcgacc
      541 tcgaccgcaa agccgagctt gcccaggcga gcgacggccc gccgcagcgg caccgccgag
      601 cgaagcacgc cggcgggcga gaagaggcgc aggcgagccg gcgtcggcac gggcgttgtc
      661 gtcatgcgtc gccgtcgagc tcgaccgact cgccggcggg aatggaaggc ggcgagacgt
      721 cggccgccat cgccaaggaa tcgtcgcccc ctgggagcgc cggcgcgccc gcgccggcag
      781 ccagcggcgc cggcagggtg gtcgcgcctc gccgctgccg gtattgctcg cgccgctcgg
      841 cgaagaagga gcgcagcagc tgcgccgacg cgtcggcaag aacgccgccg gtgaccgcgg
      901 tgtggtggtt caaggcggtg ttcgcgaaga ggtcgatgac ggagccggcg gcgcccgctt
      961 tcggatcggt cgccgcatag acgactcgct tgacccgggc gtgcagcagc gccatcgcgc
     1021 acatcgcaca gggctccagg gtgacgtaga tctcgcactc gggcagccgg tagttgccga
     1081 ggagttgcgc cgcatggcgc aatgcgacga tctcggcatg cgcggtcgga tcgtgatcgg
     1141 tgatcggccg gttgtagccg gtggccacca cttgtcggcc ggagggcgaa tcgcgcacga
     1201 tgacggcgcc gacaggcacc tcccccacca gccaggcgtt gtgcgcctga tcgatggcca
     1261 gccgcatgcc ggcttcgtcg tcgtgcggca tcagggctcg gcgcgctggt ttcgctgcat
     1321 gccctgctgc atcgcgcgca ccgcgttgtc gcggacctgg ctctcgatgt tcctcgcctg
     1381 ctgcggcacc gtgccgatgt tcggatcggc agcgacagca cccggcatgc cgccggggat
     1441 ggcgagggtg gcgccgtcgc ggtcgcccgc gggcaccgta tcggcggctg cgtcggccgc
     1501 ccggcgcgac gcctcgtcgg cgcgcgtcac gacgttgccg gggccgccgc cggcgagccc
     1561 gaccgcttgc aattgctttt tggcgatcga cccgacgatc gccaggacga gcacgaggcc
     1621 gaggatgccg aaaatcgcct tcatcatgcg agcctcccgg aaggatggtg gatcgtagcc
     1681 gctgccaccc tgacgtcggc gccgaccggc ctgtcttcag tggagcggca ggggcgtggc
     1741 gtgtccggtg agcaggcgaa gtccgaacac gatggcgccg acgccggccg cgccatagac
     1801 gacgaacatc gcccagtagc cgagcggttc ctcgtcgcga cggaactcga gccgctcggc
     1861 gaagccgcgc gaaccgcagg gcagccagcc cgcgcccagg ctgcgcaggg tcatggcgat
     1921 caggccgaca ccgatcgcca ggaagacgaa accctcgaag atgcgcatgg tcgtcagccg
     1981 gccggcccga ggttaccgtc gccgcgcggc gacaggcagg ccgatgacgg aaacgatgac
     2041 gatgccgagc gaggcgacga tcgggccgat cacgaccgcg ccgatcatcc cgcagaggtt
     2101 gcccgcccgc ggccgcgagc aggcatggtc gatcgacagg gtcgcggcgg cgaaatagag
     2161 gccgaacgcc accagcaggc cgattcccac gaaggccccg atggtgacgc gccggtcgtc
     2221 cggcaccgcc cgccgatggc ggagatagcg ggccagcgcg aagaccgtgg ccgacggcat
     2281 cgacagcaag ccgacgatca tggaaacgac aagtgccatg aagaagtccc cgccggatgt
     2341 tctcacgcag gaccgtcgtc g
//
LOCUS       DASRWS010000177         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2945775, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000177 DASRWS010000000
VERSION     DASRWS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945775"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2327)
                     /gene="ligD"
                     /locus_tag="VFF72_03210"
     CDS             complement(<1..2327)
                     /gene="ligD"
                     /locus_tag="VFF72_03210"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZV92196.1"
                     /translation="MAAASKSQAGALGHYRQKRDFSITSEPAETAATPARNGEKRAFV
                     VQKHWASRLHYDFRLELDGVLVSWAVPKGPAFDPSKMQMAIHVEDHPLAYAAFEGTIP
                     PRQYGAGRVIVWDRGSWTPVGDAQAGLAAGKLVFRLHGEKLAGLWELVRIAKPGDRQD
                     AWMLFKKRDEWARPLTEYDVIAALPDSVVARPLGPVEEREPRGPQGEPARSAGDSRPP
                     DLGAAVRAPLPARLEPQLAVLAETPPAGAWLVETKFDGYRVMARIDHGKARLITRGGH
                     DWTAKMRPLAAAVEALGIETAWLDGEIVVMNAAGVPDFSALQNAIDSAAAEDIEYFVF
                     DLPFEGGRDLRAVPLRDRRAVLEQLLEGSRSDRIRLSRTFDAPPAAMLEAARQMGLEG
                     VILKRPDSPYVSRRSDSWIKLKCSLRQEFVVCGFTDRSGAKAEVGSLLLATHEAGGLV
                     YSGNVGTGWDAKTGRDLHARLTAIETSRPTLDVASIEPGRWSRRSAGGERWVRPELVV
                     EVSFREWTPDGHIRHAVFQGLRPDKPAAEVHREYAASLAPAPAAATPKASNRTATGAS
                     APSREEKGAGKRASAGKEIESGPGPGRAASKRRVGTSPPGNAAVRLTHPERVVDVSTG
                     LTKLDVARYYESVAERILPHLKDRPVSLVRAPEGIGGELFFQKHPETRVPGLQVFAAS
                     LWPGHAALLGIDSADALLSAAQMNTIELHTWNSTARRIDSPDRVVFDLDPGEGVTWAH
                     VREAAILTRTLLTELGLASWPKTSGGKGLHVVVPIA"
     gene            complement(2348..>2697)
                     /locus_tag="VFF72_03215"
     CDS             complement(2348..>2697)
                     /locus_tag="VFF72_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92197.1"
                     /translation="AGLDFMTDVLGVAAMREWNHRLAWQSAALLCRRWGREWTTPEAM
                     IGCMVTLPLPEGLGPAEAATAQRLRDALLVEHRIEVPVIARGGSLWLRLSAQVYNDES
                     DIDRLAVAIDALR"
BASE COUNT          380 a          989 c          920 g          408 t
ORIGIN      
        1 gcgatcggca cgacgacgtg caggcccttg ccgccactcg tcttcggcca gctcgccagg
       61 ccgagctcgg tgagcaaggt ccgcgtcagg atcgccgctt cacgcacatg ggcccacgtc
      121 accccctcgc ccgggtcgag gtcgaagacg acgcgatcgg ggctgtcgat ccgccgcgcc
      181 gtcgagttcc acgtgtggag ctcgatcgtg ttcatctgcg ccgccgacag cagcgcgtcg
      241 gcgctgtcga tgcccagcag cgccgcgtgg cccggccaga gcgacgccgc gaagacctgc
      301 agccccggca cgcgcgtctc gggatgcttc tggaagaaga gctcgccacc gatgccctcc
      361 ggtgcccgga ccagcgacac cggacggtcc ttcaggtgcg gcaggattcg ctcggcgacg
      421 ctctcgtagt agcgagccac gtcgagcttg gtcagccccg tcgagacatc gacgacgcgt
      481 tcgggatggg tcagcctgac ggcagcgttg cccggcggcg acgtgccgac gcgacgcttc
      541 gaagcggcgc gtcccggccc tggccccgat tcgatttctt tgcccgctga cgctcgcttt
      601 cccgcaccct tttcctcccg cgatggcgcg ctcgcccccg tcgccgtgcg attcgaagcc
      661 ttcggcgtgg ccgccgctgg cgcaggggca agcgacgcgg cgtactcgcg atgcacctcc
      721 gcggccggct tgtcggggcg caacccctgg aagacggcgt ggcggatgtg gccatcgggc
      781 gtccactcgc gaaacgagac ctcgacgacc agctccggac gcacccagcg ctcgccgccg
      841 gcgctgcgcc tcgaccagcg gcccggctcg atcgaggcga cgtccagcgt cggccggctc
      901 gtctcgatcg ccgtcagccg cgcatgcagg tcgcggcccg tcttcgcgtc ccagccggtg
      961 ccgacgttgc cgctgtagac gagaccgccg gcctcgtggg tcgcgagcag caggctgccg
     1021 acttctgcct tcgcgccgct gcgatcggtg aagccgcaga cgacgaactc ctggcgcagg
     1081 ctgcacttca gcttgatcca ggaatcgctc cgccgcgaca cgtagggcga atcgggccgc
     1141 ttgaggatga cgccttcgag ccccatttgc cgagctgcct cgagcatcgc tgccggcggc
     1201 gcatcgaagg tccggctgag acggatccgg tcgctcctgc tgccctcgag aagctgctcg
     1261 agcaccgcgc gccggtcgcg cagcggcacc gcgcgaaggt cgcggccgcc ttcgaacggc
     1321 aggtcgaaga cgaaatattc gatgtcttcg gcggccgccg aatcgatggc gttctgcaag
     1381 gcgctgaaat ccggaacgcc ggcggcgttc atgacgacga tctcgccgtc gagccaggcg
     1441 gtctcgattc cgagcgcctc gacggcagcg gcgagcggac gcatcttcgc cgtccagtcg
     1501 tggccgcctc gcgtgatcag tcgggccttg ccatggtcga tccgcgccat gacgcgatag
     1561 ccgtcgaact tggtctcgac cagccaggcg ccggccggcg gggtctcggc cagcacggcg
     1621 agctgcggct cgaggcgcgc cggcaacggc gcccggaccg cggcaccgag gtcgggcggc
     1681 cggctgtcgc cagcgcttcg cgcaggctcg ccctgcgggc cgcgcggctc gcgctcctcg
     1741 accgggccga gcggcctggc gacgacgctg tcgggcagcg ccgcgatcac gtcgtactcg
     1801 gtcagcggcc gggcccactc gtcgcgcttc ttgaaaagca tccacgcgtc ctgccgatcg
     1861 cccggcttgg cgatgcgcac cagctcccac aggcctgcga gcttttcccc gtgcagccga
     1921 aagaccagct tgccggcggc caggcctgcc tgcgcgtcgc ccaccggcgt ccagctgccg
     1981 cgatcccaga cgatgacgcg cccggcgccg tactgcctgg gcggaatcgt cccctcgaaa
     2041 gcggcgtagg cgaggggatg gtcctcgacg tggatcgcca tctgcatctt cgacggatcg
     2101 aacgccggcc ccttcggcac cgcccagctg acgagcacgc cgtcgagctc gagcctgaag
     2161 tcgtagtgca gtcgactcgc ccagtgcttc tgcacgacga aggcccgctt ttctccgttg
     2221 cgggcgggag tcgcggcggt ttccgccggc tcggacgtga tcgagaagtc gcgcttttgc
     2281 cgatagtggc cgagggctcc ggcctgactc ttcgacgcgg cggccatggc gcggtcgtgc
     2341 cgccggctca gcgcaaggcg tcgatggcga cggcgagccg gtcgatgtcg ctctcgtcgt
     2401 tgtagacctg cgcggacagg cgcagccaaa gcgagccgcc gcgggcgatc accggcactt
     2461 cgatccgatg ctcgacgagc aaggcatcgc gaaggcgctg cgccgtcgcc gcctccgcag
     2521 gtccgaggcc ctccggcaag ggcagcgtca ccatgcagcc gatcatcgcc tcgggcgtcg
     2581 tccactcgcg gccccagcgg cggcacagca gggcagcgct ctgccatgcg agacgatgat
     2641 tccattcgcg catcgccgcg acaccgagca cgtcggtcat gaagtcgagt ccggccg
//
LOCUS       DASRWS010000178         4877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2654401, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000178 DASRWS010000000
VERSION     DASRWS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654401"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFF72_03220"
     CDS             complement(<1..539)
                     /locus_tag="VFF72_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HZV92198.1"
                     /translation="MNFPSLRTAQRQQHTVTPRLQHAVRLLQLSSLDFAQEVHDAMGR
                     NPFLEVEEAAHEAPLAEAPSGADIDLKSYGSELAADSPYERDSWQQTSSSVRTSNGDG
                     DLGALDLVAANEGLRASLRMQINVLPLSPRDHALACAIAESLDDDGYLRTPLDELAAT
                     SGMTPAVDEEEMLIALKRV"
     gene            1142..2341
                     /locus_tag="VFF72_03225"
     CDS             1142..2341
                     /locus_tag="VFF72_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HZV92199.1"
                     /translation="MKPAPVGRSSARRSPRRRAARAAAAAASRGAPARPARPRPPAGS
                     QLAQPSPSRTKKAAASPSQQAPQGLVWCSDDGPGIRRERAGNGFVYRDPAGRLIRRAG
                     ELNRIKALAIPPAYEDVWICPRENGHLQATGRDARGRKQYRYHAAWRLARDAGKFERM
                     LEFGAALPRIRARVKVGLAAPLGAVPRREAVLAALVRLLDTTLIRVGNDEYARENRSF
                     GLTTLRRRHADVRGSHLRLRFRGKSGVERDVDVDDPRVARLVRRCQAIAGQHLFQYED
                     EQHGIHALGSADVNDYIREAAGADFTAKDFRTWHGSAYALELWVEQCAGDAQCRRTAN
                     QLLGDVASRLGNTLAVCRKSYVHPRVLEALASSADAKVLARLTTKRRAGLSAAERRLL
                     KFLSHGR"
     gene            2548..3351
                     /locus_tag="VFF72_03230"
     CDS             2548..3351
                     /locus_tag="VFF72_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZV92200.1"
                     /translation="MEESLVIVGHDAGVRTIRLNRPKALNSFTGPMHGELAAALESAA
                     ADADVRCVVLTGSGRGFCAGQDLADPAVAPNPEAGGVPTDVGAAIERFYKPLALRIRS
                     MPVPVLAAVNGVAAGAGANLALCCDIVVATRSASFIQAFAKIGLVPDCGGTWLLPRLA
                     GRARAIGLSMLGDKLAAEDAERMGLIWKCVDDAAFTTEVHGLAARLASMPTRALVAAR
                     NAIDAAQDLDLAGALSHEADLQRVLGASHDYLEGVAAFMAKRPAVFRDR"
     gene            3357..3815
                     /gene="paaI"
                     /locus_tag="VFF72_03235"
     CDS             3357..3815
                     /gene="paaI"
                     /locus_tag="VFF72_03235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HZV92201.1"
                     /translation="MDQPTSPDPAAVAAHMWAADRASRGLGMRLVEVGEGRATMTMAV
                     RDDMLNGHDICHGGFITTLADSAFAFACNSRNVVTVASGLAVDFLAPARGGDVLTAVA
                     LETSRAGRTGVYDMVVTNQHGARLALVRGRSHSLAGRTVLPTNPPTQGAS"
     gene            3812..>4877
                     /locus_tag="VFF72_03240"
     CDS             3812..>4877
                     /locus_tag="VFF72_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV92202.1"
                     /translation="MTVKRPVPGELEPIERASADELRSLQLQRLQWSLKHAYDNVAHY
                     RQAFDARGVHPSDLTELSHLSRFPFTVKGDLRDNYPFGMLAVPRERVARIHASSGTTG
                     KPTVVGYTLRDVDTWADLVARSIRAAGGRPGDMVHVAYGYGLFTGGLGAHYGAERAGC
                     TVIPVSGGQTEKQVQLILDLKPQIIMVTPSYMQVLIEELVRQRVDPTSTSLEVGIFGA
                     EPWTETMRKEIETHAAIDAVDIYGLSEVMGPGVASECVETKDGPVVWEDHFYPEIIDP
                     ESGEVLPDGEEGELVFTTLTKEALPVIRYRTRDLTRLLPPTARSMRRMGKIVGRSDDM
                     LIIRGVNLFPTQIEELVLQHG"
BASE COUNT          715 a         1741 c         1698 g          723 t
ORIGIN      
        1 tgcacgcgct tgagcgcaat cagcatttcc tcctcgtcga cggccggcgt catgccgctg
       61 gttgccgcca gctcgtcgag cggcgtgcgc agatagccgt cgtcgtcgag cgattcggcg
      121 atcgcgcagg cgagcgcgtg gtcgcgcggc gacagtggaa gcacgttgat ctgcatgcgc
      181 aggctggcgc gcagtccttc gttcgccgcg acgaggtcga gcgcgccgag atcgccgtcg
      241 ccgttcgacg tgcgaacgct cgacgaagtc tgctgccagc tgtcgcgctc gtaaggcgaa
      301 tcggcggcca gctcggagcc gtacgacttg aggtcgatgt cggcccccga cggcgcctcg
      361 gcgaggggcg cttcgtgggc tgcttcctcg acttcgagga acggattgcg ccccatcgcg
      421 tcatggacct cctgggcgaa gtcgagcgaa gacagctgca gcaggcgcac ggcatgctgc
      481 aggcgcgggg tgacggtgtg ctgttgacgt tgagcggtac gcagtgacgg gaaattcatc
      541 gactctccgg attcgggacc tggggcacga ccctgcgtcc cgaaggacgt cgatcgatcg
      601 aggaaaagca acgcaacacg cgtgccacgc gcgaacgggt cgccttgccc gggagcgcga
      661 ccggacgggc gccgcgccgg cgacgggcag gcgttcgagc gggcatcgct tgcggtaagt
      721 tttgccgtga ccgggcgtcg cgtggccggc gcattcgatc ctcggcgcgt gcgcgttgcg
      781 tcgggtgacg accgccatgc ccttcgaaga caggtcgccc gagaccgcgg tggctcgcgt
      841 gcgcccgacg cggcacgtga cttgcaaacg ggccgcggac catgagcgac ccgactcctc
      901 gttcccgttt ccctcgtctc gacgcgacag cgcaggtgcc tgcgtcgccg gcgattccgc
      961 cggggcgcgg acagcccccg ccgccgccga ggcccgtgcc gccgttgcca ccgacgcccg
     1021 gctcgccgca gggtgttccg ccggcgatcg acgatcccga tccgccgggc ggcattcctc
     1081 ccgttcgcga accgccgcgg ccggccgatc cggtcatcgc ccgggtcggc gccttcgctc
     1141 catgaagccc gcacccgtcg gccgcagttc ggcacggcgc agcccgcggc ggcgagcggc
     1201 ccgagccgcg gctgcggcgg cctcgcgcgg ggcgcccgcg aggcccgccc ggccccggcc
     1261 gccagccggc tcgcagctcg cgcagccgtc accgtcgcga acgaaaaaag ccgccgcgtc
     1321 gccgtcccag caagcacctc agggcctcgt ctggtgcagc gacgacggcc ccgggattcg
     1381 acgcgagcgc gccggcaacg gcttcgtcta ccgcgatccg gcgggtcgcc tgatccgtcg
     1441 cgccggcgag ctgaaccgca tcaaggcgct cgccattccg ccggcctacg aggacgtctg
     1501 gatctgtccg cgcgagaacg gccacctgca ggcgaccggg cgcgatgcac gtggccgcaa
     1561 gcaatatcgc tatcacgccg cctggcggct ggcgcgcgac gcgggcaagt tcgagcgcat
     1621 gctcgaattc ggcgcggccc tgccgcgcat ccgcgcgcgc gtcaaggtcg gcctggccgc
     1681 gccgctcggc gcggtcccgc gccgggaggc ggtcctcgcg gcgctggtgc ggcttctcga
     1741 cacgacgttg atccgcgtcg gcaacgacga atacgcgcgc gagaacaggt cgttcggcct
     1801 gacgacgctg cgccgtcggc acgccgacgt ccgcggctcg cacctgcgcc tgcgctttcg
     1861 cggcaagagc ggcgtcgagc gcgacgtcga tgtcgacgat ccgcgcgtcg cgcggctggt
     1921 gcgacgttgc caggccatcg ccggccagca cctcttccag tacgaggacg agcagcacgg
     1981 catccatgcg ctcggctcgg ccgacgtcaa cgactacatc cgcgaggccg ccggggccga
     2041 tttcaccgcc aaggactttc gcacctggca cggctcggcg tatgccctcg agctgtgggt
     2101 cgagcaatgc gcgggcgacg cccaatgccg gcgcaccgcc aaccagctgc tcggcgacgt
     2161 tgcgagccgt ctcggcaaca ctctcgcggt gtgccgcaag tcgtacgttc acccgcgtgt
     2221 tctcgaggcg ctggcgtcgt cggccgatgc gaaggtgctg gcgcgactga cgaccaagcg
     2281 acgtgcgggc ctgagcgccg ccgaacgaag gctgctcaag ttcctctcgc acggccgctg
     2341 acgatttcga ggcgtggcct gcatcgatcc gccagcgttc gccgacggca gcggcgacga
     2401 ggatccgggt ggcaggccgc aacgccttga agcgtcgccg cagccactct atactctacc
     2461 taccggtcgg tcgaatctgt ttgggccggt cgacgccgca ggcggcgccg ccagacggca
     2521 gctccgacag aaacacacgg agacagcgtg gaagaatccc tggtcatcgt cggccacgac
     2581 gccggcgtcc gcacgatccg gctgaatcgg ccgaaggcgc tgaacagctt caccgggccg
     2641 atgcacggcg agcttgccgc ggcgctcgag tcggccgccg cggatgccga tgtccgctgc
     2701 gtcgttctca ccggcagcgg ccggggcttt tgcgccggcc aggatcttgc cgatcccgcc
     2761 gtcgccccga atcccgaagc cggcggcgtt cccaccgacg tcggcgctgc gatcgagcgc
     2821 ttctacaagc cactcgcgtt gcgcatccgg tcgatgccgg tgccggtgct ggcggcggtg
     2881 aacggtgtcg cggccggtgc cggcgccaat ctcgcactgt gctgcgacat cgtcgtcgcg
     2941 acgcgatcgg ccagcttcat ccaggcgttc gcgaaaatcg gcctggtgcc cgactgcggc
     3001 ggcacatggc tgctgccgag gctggcgggc cgggcccggg caatcggcct gtcgatgctc
     3061 ggcgacaagc tggctgccga ggacgccgaa cggatgggtc tcatctggaa gtgcgtcgac
     3121 gacgccgcct tcacgaccga ggtgcacgga ctggcggcgc ggctggcgtc gatgccgacc
     3181 cgtgcgctgg tcgccgcacg caacgccatc gacgcggcgc aggatctcga cctggcgggc
     3241 gcattgagtc acgaggccga cctgcagcgc gtgctcgggg cgtcgcacga ctatctcgaa
     3301 ggcgtcgccg cgttcatggc caagcggccg gccgtctttc gcgaccggtg atgtcgatgg
     3361 atcaaccgac gtctcccgac ccggctgccg tggccgcgca catgtgggcc gccgatcgcg
     3421 cgtcgcgcgg cctgggaatg cggctcgtcg aggtcggcga gggcagggcg acgatgacga
     3481 tggcggtgcg tgacgacatg ctcaacggcc acgacatctg ccacggcggc ttcatcacga
     3541 cgctggccga ttccgccttc gccttcgcct gcaattcgcg caatgtcgtc accgtcgcct
     3601 cgggcctggc ggtcgacttc ctcgcgccgg cgcgaggcgg cgacgtgctg accgcggtgg
     3661 ccctcgaaac gtcgcgcgcc ggccggaccg gcgtctacga catggtcgtc accaatcagc
     3721 atggcgccag gctggcgctg gtgcgcggcc gctcgcacag cctcgccggt cgcaccgtgc
     3781 tgccgacgaa cccgccgacc cagggggcat catgaccgtc aagcgacccg ttccgggcga
     3841 gctcgagccg atcgagcggg ccagcgccga cgagttgcgc agcctgcagc tgcagcgact
     3901 ccaatggagc ctgaagcacg cctacgacaa cgtggcccat taccggcagg cgttcgacgc
     3961 caggggcgtt cacccgtccg acctgaccga gctctcgcac ctcagccggt ttcccttcac
     4021 cgtcaagggc gacctgcgcg acaactatcc gttcggcatg ctcgcggtgc cgcgcgagcg
     4081 ggtcgctcgc atccacgcct cctcggggac gacgggcaag ccgacggtcg tcggctacac
     4141 gctgcgcgac gtcgacacct gggccgacct cgtcgcgcgg tcgatccgcg ccgccggcgg
     4201 ccggcccggc gacatggtgc acgtcgccta cggctatggc ctcttcaccg gcggcctcgg
     4261 tgcgcattac ggtgccgagc gcgccggctg cacggtcatt ccggtgtcgg gcggacagac
     4321 cgagaagcag gtgcagctga tcctcgacct gaagccgcag atcatcatgg tcacgccctc
     4381 gtacatgcag gtgctgatcg aggagctggt gcgccagcgc gtcgacccga cctcgaccag
     4441 cctggaggtc ggcatcttcg gtgccgagcc gtggaccgag acgatgcgaa aggagatcga
     4501 gacccacgcc gccatcgacg ccgtcgacat ctacggtctt tcggaggtga tgggcccggg
     4561 agtcgccagc gagtgcgtcg agaccaagga cggcccggtc gtctgggaag accatttcta
     4621 tcccgagatc atcgacccgg aatcgggcga ggtccttccc gacggcgagg aaggcgagct
     4681 cgtcttcacg acgctgacga aagaggcgct gccggtgatc cgctatcgca cccgcgacct
     4741 gacgcgcctc ttgccgccga ccgcgcgctc gatgcggcgg atgggcaaga tcgtcggccg
     4801 cagcgacgac atgctgatca tccgcggcgt caacctcttt ccgacgcaga tcgaggagct
     4861 ggtgctgcag cacggcg
//
LOCUS       DASRWS010000179         4150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1223146, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000179 DASRWS010000000
VERSION     DASRWS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223146"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFF72_03245"
     CDS             <1..227
                     /locus_tag="VFF72_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921236.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZV92203.1"
                     /translation="TGSFRVANDGFGGAAETFALNSFAAGNNSLVPNLIRLTNREYQD
                     FVIAVPEPETYALMIAGLMAVGFISRRRKV"
     gene            373..1074
                     /locus_tag="VFF72_03250"
     CDS             373..1074
                     /locus_tag="VFF72_03250"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HZV92204.1"
                     /translation="MKTMRVAAIAATLMCVASIGSIAARPESKAADKGKIISLETMVP
                     KTFGDWSELRDQAVQVVNPETQQLLDKLYSQTLTRTYVDKQGYRIMLSLAYGDDQRGG
                     LQAHRPEVCYPAQGFKLGRVEESLLPTPFGSIDVRRLTTTLGQRHEPVTYWLTVGDQI
                     VQSSFDRRLAQIRLGLTGQIPDGLLFRVSSIDVDDARAFAMQQKFVADLMAAVPGEAR
                     KDLGGLDAQRRPLKS"
     gene            complement(1071..1988)
                     /gene="xrtB"
                     /locus_tag="VFF72_03255"
     CDS             complement(1071..1988)
                     /gene="xrtB"
                     /locus_tag="VFF72_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase B"
                     /protein_id="HZV92205.1"
                     /translation="MSSVLPPMPAARSRGGTPDASWWVALAGFAAMYLPVYWWAAGGI
                     WQSDEQAHGALVLLVMLWLFWGLRRPIADLDAAPSPAWGWPVFVFGLFVYFVGRVFEI
                     SILEFGSQPFVVAGVLLLIRGRAVIRLAWFPLLYFVFMVPLPGILVDAVTQPLKQWIS
                     AIVVDVLYDVGYPISRTGVVLSIGQYQMLVADACSGLHSMFSLSALGTLFMYITQRKS
                     VLHNAIMVASILPIAFAANVVRVMVLVLITYHFGDAAGQGFLHGAAGIVLMLVALGFL
                     FFLDAGLARVLRPRADRRDTPSPTSRGSA"
     gene            complement(1985..2893)
                     /gene="epsG"
                     /locus_tag="VFF72_03260"
     CDS             complement(1985..2893)
                     /gene="epsG"
                     /locus_tag="VFF72_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HZV92206.1"
                     /translation="MKNTVPTIGTPPRATRSLGAILVDGGQLKAEDAERVLQYQRDHN
                     LRFGEAAMRLGLISEADIQFALSRQFSYAYLRQSPGEARVLSDELVAAFQPFSTRVEQ
                     LRAIRSQLMLRWFDKTEGRQVLTVVGPERGEGRSYLAANLAIVFSQLGERTLLVDADM
                     REPRQHFLFYLENRIGLSTLLAGRSREEAIVRIPDLAGLSVLPAGPTPPNPLELLNRL
                     NFDEFMIQAKNAYDVIIVDTPAMSIGEDAAMIAVRTGAAIAVARGGSTRVAAFTDLVR
                     GLMDAGVSVVGSVLNEVPMKRKKKAK"
     gene            complement(2890..>4150)
                     /locus_tag="VFF72_03265"
     CDS             complement(2890..>4150)
                     /locus_tag="VFF72_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HZV92207.1"
                     /translation="LAPALAAPSYMATQIEILRSDRVATRAVKLLGVERSPAAVAQWR
                     DATKAKIPLDRYFADVLEKGLVVEQARGSSVLNLSFSAPDPIFAQAAANAFAQAYMDI
                     SVELRVAPARQSANFLDDQVKTLRTNLEQAQAKLSSYQQSKGIVVSDEKIDQENARYS
                     ALIAQLALAQAEQVDTAARQRYSGGENSPDVLQSPSVQALKGQLTTAEAKLVELSKVV
                     GKNYPSRVQLAAQIDELKQQLAVEVRRVAGSASLTNRGSSQKVAELQAMVDAQKKKLL
                     SMRSDHDQIAVYQRDVETAQRAYDAVTQRLGTLNLEGQNNQANTRLLSPAVEPIEPSR
                     PKILLGIVGSLIGGLAAGLLAALGLEMRDRRVRIPEDLIVIPGVPVIGVLRPAGSRLP
                     VFRRLLMVGPSPPVRPALAGPVPRLRS"
BASE COUNT          732 a         1373 c         1391 g          654 t
ORIGIN      
        1 cgaccggttc cttccgcgtc gccaacgacg gcttcggcgg cgctgccgaa acgttcgcgc
       61 tcaacagctt cgcggccggc aacaattccc tggttccgaa cctgattcgc ctgaccaacc
      121 gcgaatacca ggatttcgtc atcgccgtgc cagaacccga gacctatgcg ctgatgatcg
      181 ctggcctgat ggcggtggga tttatctcac ggcgccgcaa ggtctgaccc gcgcgcccgg
      241 caacgagccc gtgtagagtg caacgcgcgg cgggaacccg tcttcgccat cgagacccgc
      301 aaggcgactt gtgggtctct tttcatggcg aaccgacccg ccgattcccg cgaccgccgt
      361 gaaaggctcg aaatgaagac gatgcgcgta gcggccattg ccgctacgct gatgtgcgtg
      421 gcttcgatcg gcagcattgc ggcgaggccc gagtcgaaag ctgccgacaa aggcaagatt
      481 atctcgctcg agaccatggt gccgaagacc ttcggcgact ggagcgagct tcgcgatcag
      541 gcggtgcagg tcgtcaatcc cgagacccag cagctgctcg acaagctgta cagccaaacc
      601 ctcacccgca cctacgtcga caagcaaggc tatcgcatca tgctgtcgct cgcatatggc
      661 gacgatcagc gcggcggcct gcaggcccat cgtccggagg tctgctaccc ggcacagggc
      721 ttcaagctcg gtcgcgtcga ggaatcgttg ctgccgacac ccttcggatc gatcgacgtg
      781 cggcgcctca cgaccacgct cggccaacgc catgagccgg tcacgtattg gctcacggtc
      841 ggcgaccaga ttgtccagtc atcgttcgac cggcgactcg cccaaatccg gctcggcctg
      901 accgggcaga tccccgacgg gttgctcttt cgcgtctcgt cgatcgacgt cgacgacgcc
      961 cgtgccttcg ccatgcaaca gaagttcgtc gccgacctga tggccgccgt tcccggcgag
     1021 gcgcgcaagg atctgggcgg cctggacgcg caacgacggc cgttgaaatc ctaggccgaa
     1081 ccgcggctcg tcggcgaagg cgtatcgcgg cgatccgccc gcgggcgcag cacccgcgcc
     1141 agccccgcat ccagaaaaaa gaggaatccc agggccacga gcatcagcac gattcccgcc
     1201 gcgccgtgca ggaagccctg cccggccgcg tcgccgaagt gataggtgat gagcaccagc
     1261 accatcacgc gaacgacgtt ggcggcgaat gcgatcggca ggatgctcgc caccatgatg
     1321 gcgttgtgca gaacgctctt tcgctgcgtg atatacatga agagcgtgcc gagcgccgag
     1381 aggctgaaca tcgagtgcag ccccgagcag gcgtcggcca cgagcatctg gtattggccg
     1441 atgctcagca ccacgccggt gcggctgatc ggatagccca cgtcgtagag cacgtcgacg
     1501 acgattgcag agatccattg cttgagcggc tgcgtcacgg cgtcgacgag gatgcccggt
     1561 agcggaacca tgaagacgaa gtagaggagc ggaaaccacg ccaaccggat caccgcccgg
     1621 cccctgatca gcagcaggac gccagcgacg acaaagggct gcgagccgaa ctctaggatc
     1681 gagatctcga agacgcggcc gacgaagtag acgaagaggc cgaacacgaa gaccggccag
     1741 ccccacgccg gtgaaggcgc ggcgtcgaga tcggcgatcg gtcggcgcag accccagaaa
     1801 agccagagca tcaccagcag caccagggcg ccgtgggcct gctcgtcgct ttgccagatg
     1861 ccgcccgccg cccaccaata gaccggcagg tacatcgccg cgaacccggc cagcgcgacc
     1921 caccacgacg catcgggcgt gccgcccctg cttcgggcgg ccggcatcgg cggaagaacg
     1981 ctgctcactt tgccttcttc ttcctcttca ttggcacttc gttgagcacc gagcccacca
     2041 ccgaaacgcc ggcgtccatc aggccgcgaa cgaggtcggt gaacgcggcg actcgggtcg
     2101 agccgccccg ggcgacggcg atcgcagcgc cggtgcgcac ggcgatcatc gcggcgtcct
     2161 cgccgatcga catcgccggc gtgtcgacga tgatcacgtc gtaggcgttc ttcgcctgga
     2221 tcatgaactc gtcgaagttg agacggttca ggagctcgag cggattcggc ggcgtcggcc
     2281 ctgcgggcag cacggaaaga cccgcgaggt cggggatgcg aacgatcgct tcctcgcgcg
     2341 agcggccggc gagcagcgtc gacaggccga tgcggttctc gagatagaag aggaagtgct
     2401 ggcgcggctc gcgcatgtcg gcgtcgacaa gcagtgtgcg ctcaccgagc tgcgagaaga
     2461 cgatcgccag gttggcggcg agataggagc ggccctcgcc gcgctcgggg cccacgaccg
     2521 tgagcacctg ccgcccttcg gtcttgtcga accagcgcag catgagctgg ctgcggatcg
     2581 cccgcagttg ctcgacgcgc gtgctgaagg gctggaacgc ggccacgagc tcgtcgctca
     2641 gaacgcgtgc ttcgccgggc gattgccgca ggtaggcata cgagaactgg cgcgacagcg
     2701 cgaactggat gtcggcctcg ctgatgaggc ccagccgcat cgccgcttcg ccgaagcgaa
     2761 ggttgtggtc gcgctggtat tggaggacgc gctcggcatc ttctgccttg agctggccgc
     2821 catcgaccag gatggcgccg agcgagcgcg tggcccgcgg cggggtgccg atggtcggaa
     2881 cggtgttctt cacgaacgca acctcggaac ggggccggcc agcgcaggcc gcaccggggg
     2941 ggaagggccc accatgagca gccgacgaaa caccggcaac cgcgatccgg ccgggcgcag
     3001 cacgccgatc accggaacgc cgggaatgac gatcaggtct tcgggaatgc ggacgcgccg
     3061 gtcgcgcatc tcgaggccaa gcgccgcgag caggccggcg gcgaggccgc cgatcagcga
     3121 gccgacgatg ccgagcagga tcttcggccg cgaaggctcg atcggctcga ccgccgggct
     3181 gaggagacgg gtgttggcct ggttgttctg gccctcgagg ttgagcgtgc ccaggcgctg
     3241 cgtcacggcg tcgtaggcgc gctgcgcggt ctcgacgtcg cgctggtaga cggcgatctg
     3301 gtcgtgatcg gatcgcatcg agaggagctt tttcttctgc gcgtcgacca tcgcctgcag
     3361 ctcggccacc ttctgcgagc tgcctcgatt ggtgagcgat gcactgccgg ccacccgacg
     3421 aacttccacg gcgagttgct gcttcagctc gtcgatctgc gccgcgagct gcacccgcga
     3481 cggatagttc ttgccgacca ccttgctgag ctcgacgagc ttggcctcgg ccgtggtgag
     3541 ctgacccttg agtgcctgca ccgacgggct ctgcaacacg tcgggcgaat tctcgccacc
     3601 cgagtagcgc tgccgggcgg cggtgtcgac ctgctcggcc tgggccagcg cgagctgggc
     3661 gatgagcgcg ctgtagcggg cgttttcctg gtcgatcttc tcgtcgctga cgacaatgcc
     3721 cttcgactgc tggtagctcg agagcttggc ctgcgcctgt tcgaggttgg tgcgcagcgt
     3781 cttgacctgg tcgtcgagaa agttggccga ctgccgcgcc ggcgcgacgc gcaattcgac
     3841 cgagatatcc atgtaggcct gcgcgaaggc gttggcggcg gcctgcgcga agatcggatc
     3901 gggcgccgag aacgaaaggt tcagcacgct gctgcctcgc gcctgctcga cgacgaggcc
     3961 cttttcgagc acgtcggcga aatagcgatc gagcgggatc ttggccttgg tcgcgtcgcg
     4021 ccattgcgcc accgccgccg gcgagcgttc gacaccgagc agcttgaccg cgcgcgtggc
     4081 gacccggtcg ctcctcagga tctcgatctg tgttgccatg tagctcggcg cggcgagcgc
     4141 cggagccagc
//
LOCUS       DASRWS010000180         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2654904, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000180 DASRWS010000000
VERSION     DASRWS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654904"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1168
                     /gene="dctP"
                     /locus_tag="VFF72_03270"
     CDS             155..1168
                     /gene="dctP"
                     /locus_tag="VFF72_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZV92208.1"
                     /translation="MIDRRKFTTRSLGLAAAAIAGPGLGSTALAAEFTMRISHQFPPA
                     HQTAREFEQFARDVKSATHGRVEVQVFGAAQLFKPNQNHPAVASGKIEAASILNFQWG
                     KTISEMSVTIIPYFITSVARQRAFIASDAARQLEAKMEAHGVKCIAWIVDTNDGIFTS
                     QKKPLLTPADFAGLKIRGLNRLFDAGLVAMGASPSPMPGTEVYQALQTGILDAGFTGV
                     EAANSRKFYEVQKYGVASNIILAFDNLVVNPAWWNGLPPDIRGAIRKCADQAVQRSIH
                     KTDGIDPKTIEVLNEKGMKAVALTKAQERVMADAMQPAVKREFLIETGPDGVKLLQTI
                     DRL"
     gene            1174..1743
                     /locus_tag="VFF72_03275"
     CDS             1174..1743
                     /locus_tag="VFF72_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZV92209.1"
                     /translation="MGKTGEGDVASESRTLRLLGRAVAALATAAMAVAALALLLSLAL
                     IGWAVVMRYAFNAAPIWVDDVVGFLLVAIVMLAAADTLRRGEHIGVDLLVTNLPLRRR
                     RWVAAWAAFATALIAGVLVWNGWGAAMLAKTLGMVTEGALEWPSWLLMLLMPLGGMLL
                     LLTAVEAFWRAIAGAPPAVAPHALPTDAE"
     gene            1740..3026
                     /locus_tag="VFF72_03280"
     CDS             1740..3026
                     /locus_tag="VFF72_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZV92210.1"
                     /translation="MTVALIFAGLLILLFSGLPIFAGLALFGGGLLYLTQGELGSVTE
                     VIFGELNRYLLVAIPLFAFMAQIMIRGKIVDDLYHTAYTLTRHLPGGLGIATIAACTI
                     FAAISGSSVATALTIGAVAIPQMIRYGYDAKAAYGLVAAGGTLGILIPPSGPMVLYGV
                     TTDTSIGGLFMAGVLPGLLMAGVFAAWSMASTAVAHRHIAREPRASLREALVAIKKAL
                     WALTLPVFVLGGMYAGIFTATEAAAAGAWLALFIAVVVYRTVGWRAVWEASLDACRVS
                     AMLFMILAGAAVFGHVLTKLRLPQQIVQAVISADIGVTGFLVVMMALIFVLGMFLESI
                     SIILITTPVILPVMAQLHIDAIWYGVLLVINLELAQITPPVGMNLFTIKAITGAPLGP
                     IVRGSLPYVLLMIAVMALVMIWPQIALWLPSTMMRR"
     gene            complement(3048..>3377)
                     /locus_tag="VFF72_03285"
     CDS             complement(3048..>3377)
                     /locus_tag="VFF72_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HZV92211.1"
                     /translation="WMWFVFPQLRGLGTSVMAERYGVSGLAEAGAYLRHPLLGERLVD
                     CTRLVCAIPDRSLGEIFGSPDDLKFVSSMTLFDAAGSGVAVFADALRQHAGATRDAAT
                     LRLLGRA"
BASE COUNT          520 a         1200 c         1106 g          551 t
ORIGIN      
        1 gagcgactcc cgatccggct gcagccgccc cgccttggcg agcacaccca ggagttgctc
       61 gccggcctcg gctactcacg cgaagagatc gagacgctgc gcgcgcagcg cgccgtcgca
      121 tgaatccccc gaccgaaccc accaggagcc cgcgatgatc gaccgccgca agttcaccac
      181 ccgctcgctc ggcctcgccg ccgcggcgat cgccggaccg ggcctgggct cgaccgccct
      241 cgccgccgag ttcacgatgc gcatcagcca ccagttcccg ccggcgcacc agaccgcgcg
      301 cgaattcgag cagttcgcca gggacgtgaa atcggcgacg cacgggcgcg tcgaggttca
      361 ggtgttcggc gcggcccagc tcttcaagcc gaaccagaac catccggcgg tggcgagcgg
      421 caagatcgaa gcggcatcga tcctcaactt ccagtggggc aagacgatct cggagatgag
      481 cgtcaccatc attccgtact tcatcacctc ggtcgcccgg cagcgggcct tcatcgcatc
      541 cgacgccgca cgccagctcg aggcgaagat ggaggcgcac ggcgtcaagt gcatcgcctg
      601 gatcgtcgac accaacgacg gcatcttcac ctcgcagaaa aagcccctgc tgacgccggc
      661 ggacttcgcc ggactgaaga tccgcggcct caaccggctc ttcgacgccg gtctcgtggc
      721 gatgggcgcg tcgccatcgc cgatgcccgg caccgaggtc taccaggcac tgcagacggg
      781 cattctcgat gccggattca ccggcgtcga ggccgcgaac agccgcaagt tctacgaagt
      841 gcagaagtac ggcgtcgcct cgaacatcat cctcgccttc gacaacctgg tcgtcaatcc
      901 ggcgtggtgg aacggcttgc cgccggacat ccgcggtgcc atccggaagt gcgccgacca
      961 ggccgtgcaa cgctcgatcc acaagaccga cggcatcgac ccgaagacga tcgaggtgct
     1021 gaacgagaaa ggcatgaagg cggtggcgct gacgaaggcg caggaaagag tgatggcaga
     1081 cgcgatgcag cccgccgtga agcgcgagtt cctgatcgag accgggcccg acggcgtaaa
     1141 gctcctgcag acgatcgaca ggctctgaat cgcgtgggca agaccggcga gggcgacgtc
     1201 gcgtccgaga gccggacgct gcgcctgctc ggccgcgccg tcgccgcgct ggcgacggcg
     1261 gcgatggccg tcgccgccct ggccctcctc ctttcgctgg cgttgatcgg ctgggccgtc
     1321 gtcatgcgct acgccttcaa tgcggcgccg atctgggtcg acgatgtcgt cggcttcctg
     1381 ctcgtcgcca tcgtgatgct cgccgcggcc gacacgctgc gtcgcggcga gcacatcggc
     1441 gtcgacttgc tcgtcacgaa tctgccgttg cgccggcggc gctgggtcgc tgcctgggcg
     1501 gcgttcgcca ccgcgctgat cgcaggcgtt cttgtctgga acggctgggg cgccgcgatg
     1561 ctcgcgaaga cgctcggcat ggtgaccgag ggcgctctcg aatggccgag ctggctgctc
     1621 atgctgctga tgccgctagg cggcatgctg cttctgctca ccgccgtcga ggcgttctgg
     1681 cgggcgatcg caggcgcgcc gcccgcggtc gcgccgcacg ccttgccgac cgacgccgaa
     1741 tgaccgtcgc cctcatcttc gccggcctgc tcatcttgct cttttccggg ctgccgatct
     1801 tcgccgggct cgccctcttc ggcggcggcc tgctctacct cacgcagggg gagctcggct
     1861 cggtcaccga ggtgatcttc ggcgagctga accgctacct gctcgtggcg atcccgctct
     1921 tcgccttcat ggcgcagatc atgatccgcg gcaagatcgt cgacgatctc taccacaccg
     1981 cctacacgct gacgcgccat ctgccgggcg gcctgggcat cgcgacgatc gcggcctgca
     2041 cgatcttcgc cgccatctcg ggctcgagcg tggcgacggc gctgacgatc ggcgcggtcg
     2101 ccatcccgca gatgatccgc tacggctacg acgcgaaggc cgcctacggc ctcgtcgccg
     2161 ccggcggcac gctcggcatc ctcattccgc cgtcggggcc gatggtcctc tacggcgtca
     2221 ccaccgacac gtcgatcggc ggcctgttca tggctggcgt gctgcccggg ctgctcatgg
     2281 ccggcgtgtt cgcggcgtgg agcatggcga gcaccgcggt cgcgcatcgg cacatcgcgc
     2341 gcgagccgcg tgccagcctc cgcgaagcgc tcgtcgcgat caagaaggcg ctctgggcgc
     2401 tgacgctgcc ggtgttcgtg ctcggtggca tgtacgccgg cattttcacc gccaccgagg
     2461 cagccgcggc aggcgcctgg ctggcgctct tcatcgccgt cgtcgtctac cggacggtgg
     2521 gctggcgcgc cgtgtgggag gcgtcgctcg acgcctgccg cgtctcggcg atgctcttca
     2581 tgatcctcgc cggcgccgcc gtcttcggcc atgtcctcac caagctgcgc ctgccgcagc
     2641 agatcgtgca ggcggtcatc tcggccgaca tcggcgtcac cggatttctc gtcgtcatga
     2701 tggcgctgat cttcgtgctc ggcatgttcc tcgagtcgat ctcgatcatc ctcatcacca
     2761 cgccggtgat cctgccggtg atggcgcagc tccacatcga cgccatctgg tatggcgtgc
     2821 tgctcgtcat caacctcgag ctggcgcaga tcacgccgcc ggtcggcatg aacctgttca
     2881 ccatcaaggc gatcaccggc gcgccgctcg ggccgatcgt tcgcggctcg ctgccctacg
     2941 tgctgctcat gatcgcggtg atggcgctgg tcatgatctg gccgcagatc gcgctctggc
     3001 tgccaagcac catgatgcga cgctaggacg caccgcacgc cggtgcttca ggcgcgaccc
     3061 agaagccgga gcgtcgccgc gtcgcgcgtt gcgccggcat gctgccggag cgcgtcggca
     3121 aacacggcga cgccggagcc ggcggcatcg aaaagcgtca tcgaggagac gaacttgaga
     3181 tcatcgggcg agccgaagat ctcgcccagc gaccggtcgg gtattgcgca gacgagccgc
     3241 gtgcaatcga cgaggcgttc gccgagcagc gggtgacgga gataggcccc ggcctcggca
     3301 agacccgaga ccccgtagcg ctcggccatg acgctggtgc cgagcccgcg caattgcgga
     3361 aagacgaacc acatcca
//
LOCUS       DASRWS010000181         3421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4098796, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000181 DASRWS010000000
VERSION     DASRWS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4098796"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VFF72_03290"
     CDS             <1..1857
                     /locus_tag="VFF72_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZV92212.1"
                     /translation="LVSSVNEVAASIEQVAASADSLAGAVRQITTSVRDSGASVERVS
                     ATAQEMTAGADQVATSMSELTASVRSVSADTDTQVSSVNETAAAIEQMSRSIQGVAAG
                     SDDLAASAEETSSSISEVAASIEEVTAMTEGLASVVEQNVAATEQLSRSVQSVAQSGQ
                     RISEAADGAATSAAQLERTSQSVATLAKDADEATRRAGRDAEEGAVSVQRSIQGFTRV
                     RESVAQSTTVIREMGKQANNISGIVDTINLIAERTNLLSLNASIEAARAGDAGRGFAV
                     VAEEIRNLADRSAKATSDIAAIIKALQSVAQEAVASSNEGLRVMDESSVQAEEGANGL
                     RKILTSVSQATQTVAQIARGAQEQRDASRNVVTAISSTVEQARLIATSTAEQARSVTS
                     LVQANGQVRKTTLEVKKAMGEQARAARDILKAAQATREQAAAVRKATAEQAKTTTLIA
                     KAADTMRKGAAATARSLAEQSVAGEQISRAADSLRALSGAVARAMAEQTQAMREMSAS
                     ADTIRTQTDQTARAVKEQAKTLQGMTAGAHTTLKQIKLIAHANREHSTVSASVLSALS
                     DARQITDRNANGVQRTRGGADDLQRRTEALVTMMSRGADRPAGRRKGSNGRA"
     gene            1854..>3421
                     /locus_tag="VFF72_03295"
     CDS             1854..>3421
                     /locus_tag="VFF72_03295"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZV92213.1"
                     /translation="MTVGIFTVDARLVVQTWDPWMAEATGIPADAALGRPLADVVPDL
                     EARGVLAHLRSVLEHGTVEVLAPALHHYLIACPPRTPSGRFDRMQQHVTIGPLRREAA
                     IAGVVVTVQDVTARIESERALAGSIDAMAGTLGDPQWTNRQRNVRQLASHGQAIVETL
                     VDTLRTQHRNFSVLSSALDLLAMADLDVVDPVIACLDDQDPDLRVQAALILGRHSDPR
                     AVPALMRALSDPDLNVRFHAIEALGTLQATPAVDALIRIAETREFFLAFPAIQALARL
                     GDADTARRLLPLLGDELIGPAAVDVLGELGDDDVAAPLAELLGEPQAPVEAIAEALSS
                     LFDRYERRYGAGDQIATIVRRTATPAAVQQLVDAATRAASDRLRGIARVLGWLEGPSV
                     ERALARLLGQPSVRAQIVEALVRFGAGVVDLLIEQLGGGDVETRQAAAVALGRIGDHR
                     ATTSLVAALDDSALTVPAAAALARIGDSAAFDALMARIGDPDPAVRQAVIAALNSIGH
                     PDMPRRVLALMADS"
BASE COUNT          537 a         1223 c         1215 g          446 t
ORIGIN      
        1 ctggtgtcgt cggtcaacga ggtcgcggcg tccatcgagc aggtggcggc cagcgccgat
       61 tccctcgccg gcgccgtccg ccagatcacg acctcggtgc gcgactccgg cgcctccgtc
      121 gagcgtgtgt cggcgacggc gcaggagatg accgccggcg ccgaccaggt cgcgacgtcg
      181 atgagcgagc tcacggcgtc ggtgaggtcc gtgtcggccg acaccgatac gcaggtgtcg
      241 tcggtcaacg aaaccgccgc cgcgatcgag cagatgtcgc ggtcgatcca gggcgtcgcc
      301 gccggcagcg acgacctggc cgcctcggcc gaagagacct cgtcgtcgat cagcgaggtc
      361 gccgcgtcca tcgaagaagt gacggcgatg accgaaggcc tggcctcggt cgtcgagcag
      421 aacgtcgccg cgaccgagca gctgtcgcga tcggtgcaga gcgtcgcgca gagcggccag
      481 cgcatctccg aggcggcgga cggcgcggcg accagcgccg cgcagctcga gcgcacgagt
      541 cagtcggtgg cgacgctggc caaagacgcc gacgaagcga cccgccgcgc gggccgcgac
      601 gccgaagaag gcgccgtcag cgtccagcga tcgattcagg gcttcacgcg cgtccgcgaa
      661 tcggtcgcgc agtcgacgac cgtcatccgc gagatgggca aacaggcgaa caacatcagc
      721 ggcatcgtcg acacgatcaa cctgattgcc gagcggacga acctgctgtc cctcaacgcg
      781 tcgatcgaag cggcccgtgc cggtgacgcc ggccgcggct tcgccgtcgt cgccgaggag
      841 atccggaacc tcgccgaccg ctcggcgaaa gcgacctccg acatcgccgc gatcatcaag
      901 gcgctgcagt cggtggccca ggaggcggtc gcgtcgtcga acgaggggct gcgcgtgatg
      961 gacgagagca gcgtgcaggc cgaagaaggc gcaaacggcc tgcgcaaaat tctgacgagc
     1021 gtcagccagg cgacgcagac ggtggcgcag atcgcccgcg gcgcgcagga acagcgtgat
     1081 gcgtcgagaa acgtcgtcac ggcgatctcg agcacggtcg aacaggcccg cctgatcgcc
     1141 acgagcaccg ccgaacaggc gcgctcggtc acgagcctgg tgcaggcgaa cggccaggtg
     1201 cgcaagacca cgctcgaagt gaaaaaggcg atgggcgagc aggcccgcgc cgcgcgcgac
     1261 atcctcaagg ccgcgcaggc gacgcgcgag caggccgccg ccgtacgcaa agcgacggcc
     1321 gagcaggcga aaacgaccac cctcattgcc aaggccgccg acacgatgcg caagggcgcg
     1381 gcggcgacgg cgaggtcgct cgccgagcag tccgtcgccg gcgagcagat ttcacgggcg
     1441 gccgacagtc ttcgcgccct gagcggagcc gtggcgcggg cgatggccga acagacgcag
     1501 gccatgcgcg agatgtcggc ctcggccgac accattcgca cgcagaccga ccagaccgcg
     1561 cgggcggtga aggaacaggc caagacgctg cagggcatga ccgccggcgc ccacaccacg
     1621 ctgaagcaga tcaaactgat cgcccatgcg aaccgcgagc actccacggt gtcggcgtcg
     1681 gtgctgtcgg cgctctccga cgcgcggcag atcaccgatc gcaacgcgaa cggcgtccag
     1741 cggacgcgcg gcggcgccga tgacctgcag cgccggaccg aggcgctcgt cacgatgatg
     1801 tcgcgcgggg ccgaccgtcc ggcgggacgg cgcaaggggt ccaacggtcg ggcgtgacgg
     1861 tcggcatctt caccgtcgat gcgcggctcg tcgtgcagac gtgggatccg tggatggccg
     1921 aggccaccgg cattccggct gacgcggccc tcggccgtcc gcttgcggac gtcgtaccgg
     1981 acctcgaggc tcgaggcgtc ctggcgcacc tgcgttcggt cctcgagcac ggcaccgtcg
     2041 aggtgctcgc tccggccctg catcattacc tgatcgcctg tccgccgcgg acgccgtcgg
     2101 ggcggttcga tcgcatgcag cagcatgtga cgatcggccc gctcaggcgc gaggccgcca
     2161 tcgccggcgt cgtcgtgacg gtgcaggacg tgacggcgcg catcgagtcg gagcgcgcgc
     2221 tcgccggatc gatcgacgcg atggccggca cgctcggcga tccgcagtgg acgaaccggc
     2281 aacggaacgt cagacagctc gcgtcgcacg gccaggccat cgtcgagacc ctggtcgaca
     2341 ctcttcgcac gcagcatcgc aacttcagcg tgctgagcag cgcgctcgat ctgctcgcga
     2401 tggcggatct cgacgtcgtc gatccggtga tcgcctgtct cgacgatcag gatccggatc
     2461 tccgcgtcca ggccgcgctc attctcggcc ggcacagcga cccccgcgcg gttccggcgc
     2521 tgatgcgcgc gttgtccgat ccggacctca acgtccggtt tcacgccatc gaagcgctcg
     2581 gcaccctgca ggccacgcct gccgtcgacg cgctcatccg gattgccgag acgcgcgagt
     2641 tcttcctggc gtttccggcc attcaggcgc tcgcgcgtct gggcgacgcg gacacggcgc
     2701 gccggctgct gcccctgctc ggcgacgagc tcatcggtcc tgccgccgtc gacgtcctcg
     2761 gtgaactcgg cgacgacgac gtcgcggcgc cgctcgcgga gctgctcggc gagccgcagg
     2821 cgccggtaga agccatcgcc gaggcgctgt cgtcgctgtt cgatcgctac gaacgccggt
     2881 acggcgccgg cgaccagatc gcgaccatcg tgcggcggac ggcgacgccg gcggccgtgc
     2941 agcagctcgt cgacgccgcg acccgcgccg cgtcggatcg cctgcgcggc atcgcgcgcg
     3001 tccttggctg gctcgagggg ccgtcggtcg agcgggcgct ggcgcggttg ctgggccagc
     3061 cgtcggtgcg cgcgcagatc gtggaggcgc tggtccgctt cggagccggc gtcgtcgatc
     3121 tgctgatcga gcagctcggc ggcggcgacg tggagacccg tcaggcggcc gccgtcgcgc
     3181 tcggccgcat cggagaccac cgcgccacca cttcgctcgt ggccgcgctc gacgactccg
     3241 ctctcaccgt cccggccgcc gcggcgctcg cccgcatcgg cgactccgcg gcattcgatg
     3301 cgctcatggc gcgcatcggc gatcccgatc cggcggtccg ccaggcggtc atcgcggccc
     3361 tcaactcgat cggccatccc gacatgccgc gccgcgtgct ggcgctgatg gccgactccg
     3421 a
//
LOCUS       DASRWS010000182         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3523264, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000182 DASRWS010000000
VERSION     DASRWS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3523264"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFF72_03300"
     CDS             complement(<1..200)
                     /locus_tag="VFF72_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZV92214.1"
                     /translation="MPAEAAMANDARRLSGDALAAALRDSRATTLRRVLDASDDAWLV
                     PRQRGINPVAWELGHLAWFAEF"
     gene            344..1426
                     /gene="selD"
                     /locus_tag="VFF72_03305"
     CDS             344..1426
                     /gene="selD"
                     /locus_tag="VFF72_03305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZV92215.1"
                     /translation="MTADTDSIACAMPAQALRLTSFSHGGGCGCKIAPGVLSEILRSS
                     AGGIVPKELLVGIETADDAAVYQLNDEQALIATTDFFMPIVDDPFDFGRIAATNAISD
                     VYAMGGRPIMALALVAMPIDKLSVAQIGQVIQGGESVCRAAGIPIAGGHTIDSVEPIY
                     GLVVMGLVHPKKVRRNSDAREGDVLILGKPVGVGVLSAALKKNQLDDAGYKRLIDTTT
                     RLNTPGIALADMAAVHAITDVTGFGIAGHALEMARGAGLRAEVSWSKVPLLEGVGAMA
                     RDGVVTGASGRNWAGYGADVAIDAGLPAEARALLTDPQTSGGLLVSCDAAAVPAVLDI
                     FRADGFDSAAVIGTMRRGPAGLHIVA"
     gene            1465..2439
                     /gene="senB"
                     /locus_tag="VFF72_03310"
     CDS             1465..2439
                     /gene="senB"
                     /locus_tag="VFF72_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HZV92216.1"
                     /translation="MQRPAICIVTPALADAGNGNWRTARRWAGMLASDFRVELSASWQ
                     GGDARLLIALHARRSADSIRRWAEQMPHRPLVVVLTGTDVYRDIHRDESARRSLQLAD
                     RLVVLQERALDELAPEFRDKAIVCHQSTPERSALAKTNRHLRALMVGHLREEKDPQTY
                     FDAAALLGRRDDIRLDHVGSALDESWAAKARAARAAWPRYRWLGPLDHAATLARIRRA
                     HVLVHPSRMEGGAHVVMEAVRCGTPVLASRIPGNVGLLGEDYPGYFAVGDATALAAGL
                     ERCRDDEAFLPELARHCAARSPLFDPLVEKATLQAMVSSLLARSDGER"
     gene            complement(2453..>3050)
                     /locus_tag="VFF72_03315"
     CDS             complement(2453..>3050)
                     /locus_tag="VFF72_03315"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZV92217.1"
                     /translation="GTQALNRLIVACGVSSSLEGVPGIDLAVFWRDSLVAATAASKLA
                     PRLQADAESAYVCGLLHGTGHLILCRTYPDIANAMFTGFAVLRGAELAATEAECFGID
                     HGAVGALWVDTLGFPQEVADTIRKATQPVGDSRTPLDLTLRGACALAASIANGEAADA
                     ALAALPPAVLATFRGADGRPDAAFAKLYDALAETASSL"
BASE COUNT          441 a         1119 c         1085 g          405 t
ORIGIN      
        1 cagaactcgg cgaaccaggc caggtggccg agctcccacg cgaccggatt gatgccgcgc
       61 tggcgtggaa cgagccaggc gtcgtcgctc gcatcgagga cgcggcgcag cgtcgtcgca
      121 cggctgtcgc gcaaggcggc agcgagcgcg tcgcccgaca ggcgccgggc gtcgttcgcc
      181 atggcggctt cggcgggcat ggcgcgcagt gtgccagcgc gccgccacgg cgtgctcggc
      241 cagccgccca gacccccggc ggcgacgcgg ccaccccagg cgggacgcac cgcaatggcc
      301 gccgcgggcg acaatgccgc ctcattccac gagcgagcca cccatgaccg ccgacaccga
      361 ttcgatcgcc tgcgcgatgc ccgcgcaggc gctgcgcctg acgagctttt cccacggcgg
      421 cggctgcggc tgcaagatcg ctcccggcgt gctctcggaa atcctccgct cgagcgccgg
      481 cgggatcgtt cccaaggagc tgctggtcgg catcgagacc gccgacgacg ccgccgtcta
      541 ccagctcaac gacgagcagg cgctcatcgc cacgaccgac ttcttcatgc cgatcgtcga
      601 cgacccgttc gacttcggcc gcatcgccgc gaccaacgcg atcagcgacg tctacgcgat
      661 gggcggccgg ccgatcatgg cgctggcgct ggtggcgatg ccgatcgaca agctgagcgt
      721 cgcgcagatc ggccaggtga tccagggcgg cgaatcggtg tgccgcgctg ccggcattcc
      781 gatcgccggc ggtcacacga tcgactcggt cgagccgatc tacggcctcg tcgtcatggg
      841 cctcgtccac ccgaagaagg tgcgccgcaa ttccgatgcg cgcgaaggcg acgtgctcat
      901 cctcggcaag cccgtcggcg tcggcgtcct ttcggcggcg ctcaagaaga accagctcga
      961 cgacgccggc tacaagcggc tcatcgacac gacgacgcgc ctgaacacgc cgggcatcgc
     1021 gctggccgac atggcagccg tgcacgccat caccgacgtg accggcttcg gcatcgccgg
     1081 ccacgcgctc gagatggcgc gtggcgccgg cttgcgcgcg gaagtctcct ggtcgaaggt
     1141 gcccctcctc gaaggcgtcg gtgcgatggc ccgggacggc gtcgtcaccg gtgcgtcagg
     1201 ccgcaactgg gcaggctatg gcgccgacgt ggcgatcgac gccgggctgc ccgccgaggc
     1261 ccgcgccctc ctcaccgatc cgcagacctc gggtggcttg ctcgtcagct gcgacgccgc
     1321 cgccgtcccc gccgtcctcg acatcttccg cgccgacggc ttcgacagcg cggcggtgat
     1381 cggcacgatg cggcgcgggc cggccggcct gcacatcgtc gcctgaccgg cggctcggcg
     1441 caccggcgct tcggtgacac gccgatgcag cggccggcca tctgcatcgt caccccggcg
     1501 ctggccgacg ccggcaacgg caactggcga acggcccggc gctgggccgg catgctggcg
     1561 agcgactttc gcgtcgagct gagcgcctcg tggcaaggcg gcgacgcccg gctgctcatc
     1621 gccttgcacg ccaggcgatc ggccgattcg atccgccgtt gggccgagca gatgccgcac
     1681 cggccgctcg tcgtcgtcct caccggcacc gatgtctacc gcgacatcca tcgcgatgaa
     1741 agcgcgaggc gatcgctgca gctggccgat cgcctggtgg tgctgcagga acgtgcgctc
     1801 gacgagctgg cgcccgaatt ccgcgacaag gcgatcgtct gccaccagtc gacgcccgaa
     1861 cggtccgccc tggcgaagac gaaccgacac ctgcgcgcgc tgatggtggg ccacctgcgc
     1921 gaggaaaagg atccgcagac ctacttcgac gccgctgccc tgcttggccg gcgcgacgac
     1981 atccggctcg atcacgtcgg ctcggccctc gacgaatcgt gggcggcgaa ggcgcgcgcc
     2041 gcccgggccg catggccgcg ctaccgctgg ctcgggcccc tcgaccatgc cgcaacgctg
     2101 gcccggatcc ggcgcgcaca cgtcctcgtt cacccgagcc ggatggaagg cggcgcccac
     2161 gtcgtcatgg aggcggtgcg ctgcggcacg ccggtgctgg cgtcgcgcat ccccggcaac
     2221 gtcggcctcc tcggcgagga ctatccgggc tacttcgcgg tgggcgacgc gacagcgctg
     2281 gccgcagggc tcgagcgatg ccgcgacgac gaggcgttcc tgccggagct cgcccgccat
     2341 tgcgcggcgc gctcgccgtt gttcgatccg cttgtcgaga aggcgacgct gcaggcgatg
     2401 gtgagctcgc tgctcgcgcg cagcgacggc gagcgctgat cgcgcgccgg cgtcaaagcg
     2461 aggacgctgt ctccgccagc gcgtcgtaga gcttggcgaa cgcggcgtcc ggccggccgt
     2521 cggcaccgcg aaacgtcgcc agcaccgccg gcggcagcgc cgccagcgcc gcgtcggcgg
     2581 cctcgccgtt ggcgatcgag gcagccagcg cacaggcgcc gcgcagcgtc aggtcgagcg
     2641 gcgtgcggct gtcgccgaca ggctgcgtcg ccttgcggat ggtgtcggcg acctcttgcg
     2701 gaaagccgag cgtatcgacc cagagcgcac cgacggcgcc gtggtcgatg ccgaagcact
     2761 cggcctcggt ggcggcgagc tcggccccgc gcaggaccgc gaagccggtg aacatggcgt
     2821 tggcgatatc ggggtaggtg cggcacagga tcaggtggcc ggtgccgtgc aggaggccac
     2881 agacgtaggc gctctccgcg tcggcctgca ggcgcggcgc gagcttgctc gcggcggtcg
     2941 cggcgacgag cgaatcgcgc cagaagacgg ccagatcgat ccccggcacg ccctcgagcg
     3001 acgacgagac gccgcaggcg acgatgagtc gattcagcgc ctgcgtgccg
//
LOCUS       DASRWS010000183         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1514941, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000183 DASRWS010000000
VERSION     DASRWS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514941"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFF72_03320"
     CDS             <1..653
                     /locus_tag="VFF72_03320"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HZV92218.1"
                     /translation="AAATTATILIGEFDVDRAVFTGTAGGLHPQARVGDIVVADALLQ
                     HDMDATPLFPRHEVPLYGVDRFAADPVLSAALATAAGVVLDAASHEAHDAADAVAEAR
                     PAATQAAVDGQLLREFGIASPRVHRGLVVSGDRFVASGAESAELRHRLPDALAVEMEG
                     AALAQVCHDFGVPFAVVRTISDRADDSAHVDFSRFVAAVASRYTLAIMRRWLRNDG"
     gene            661..1434
                     /locus_tag="VFF72_03325"
     CDS             661..1434
                     /locus_tag="VFF72_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZV92219.1"
                     /translation="MLRADALSILIDADADRKASRARALDLDAPAGSDAALAEPGGIP
                     GRPERPPLVAHTEVRHRSMATAEGRAALVHALAHIELNAIDLALDLCWRFAGMPDAFY
                     RQWAGVAKEEALHFELLRDHLKSLGFAYGDFPAHNALWEMAERTKHDLLARVALVPRT
                     LEARGLDASPAIKAKLVAAGDRRAGAILDVILRDEIGHVATGNRWYRTLCAERSLDPV
                     ATYDTLAARYRAPRLRGPFNLEARRAAGFDEAELQALGR"
     gene            1573..1881
                     /locus_tag="VFF72_03330"
     CDS             1573..1881
                     /locus_tag="VFF72_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZV92220.1"
                     /translation="MAKLFAIYQQPKDPAAFDRYYFDKHLPLAKTIPGLKSYEVTRGD
                     VMGMGGKHGVYLIAILEFASMQAIGEAMASAEGQATAADLANFADAGVDVMMAETHLV
                     "
     gene            1985..>2323
                     /locus_tag="VFF72_03335"
     CDS             1985..>2323
                     /locus_tag="VFF72_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92221.1"
                     /translation="MDAERLSRRWYLVAITYFAIGIAFGVYMGASGNHSLFTVHAHIN
                     LLGWASMALTGLLYRSFPAAARSRLAVWHFWLYQAAVPVMLVAVVLILSGTKAAEPVA
                     GIASVVILISV"
BASE COUNT          349 a          836 c          774 g          364 t
ORIGIN      
        1 tcgcggcggc gacgacggcg acgatcctga tcggcgagtt cgacgtcgac cgcgccgtct
       61 tcaccggcac tgcgggcggc ctgcatccgc aggcccgcgt cggcgacatc gtcgtcgccg
      121 acgccctgct ccagcacgac atggacgcca cgccgctctt tccgcgccac gaagtgccgc
      181 tctacggcgt cgaccggttc gccgccgacc ccgtactttc ggcggcgctg gccacggccg
      241 ccggtgtcgt cctggacgcc gcaagccacg aggcacatga cgcggccgac gcggtcgccg
      301 aggctcgccc ggcagccacg caagccgccg tcgacgggca gctgttgcgc gaattcggca
      361 tcgcatcgcc ccgcgtgcat cgcggcctcg tcgtcagcgg cgatcgcttc gtcgccagcg
      421 gcgccgagag cgccgagctc cgccatcggc tgccggatgc actcgcagtc gagatggaag
      481 gcgcggcgct ggcccaggtc tgccacgact tcggcgtgcc gttcgccgtc gtgcgcacga
      541 tctcggaccg cgccgacgat tcggcccacg tcgacttctc gcgcttcgtc gccgcggtgg
      601 ccagccgcta cacgctggcg atcatgcggc gctggctgcg caacgacggc tgagccagcg
      661 atgcttcgag ccgacgcgct gtccatcctg atcgacgccg atgccgatcg caaggcgagc
      721 cgggcccgcg cgctggacct cgacgcgccc gcgggcagcg acgccgcgct ggccgagccc
      781 gggggcatcc ccggccggcc ggagcggccg ccgctcgtcg cccacaccga ggtccggcat
      841 cgatcgatgg cgacggccga gggccgcgca gcgctcgtcc atgcgctcgc ccacatcgag
      901 ctcaacgcca tcgatctggc gctcgacctg tgctggcgat tcgccgggat gcccgacgcg
      961 ttctatcggc agtgggccgg cgtcgccaag gaagaggcac tccacttcga gctgttgcgc
     1021 gaccatctga agtcgctggg cttcgcctac ggcgacttcc cggcccacaa cgcgctctgg
     1081 gagatggccg agcggaccaa gcacgacctg ctggcacggg tcgcgctggt gccgcgaacg
     1141 ctcgaggcgc gcggcctcga cgcgtcgcct gcgatcaagg ccaagctcgt cgcggcggga
     1201 gatcgtcgcg ccggcgccat cctcgacgtc atcctgcgcg acgagatcgg ccacgtcgcg
     1261 accggcaatc ggtggtaccg gacgctgtgt gccgagcgct cgctcgaccc ggtggccacg
     1321 tacgacacgc tcgctgcgcg ctaccgtgcg ccacgccttc gcggcccgtt caatctcgag
     1381 gcgcgccgcg ccgccggctt cgacgaggcc gagttgcagg cgctcggacg ctgaacgccg
     1441 ggcccggccg tcacccgcac cacgacactg ccgatcaggg caaacctgat tcaggacatc
     1501 ggacggtcct gtcaccatcg acgtccccga gcttcgcctc ccatccggcg gccggccacc
     1561 aaaggacacg tcatggcaaa gcttttcgcg atctaccagc agccaaagga tccggcggcg
     1621 ttcgaccgct actacttcga caagcatctg ccgctggcga agacgatccc cggcctgaag
     1681 tcgtacgaag tcacgcgtgg cgacgtcatg ggaatgggcg gcaagcacgg cgtctacctg
     1741 atcgcgattc tcgagtttgc ctcgatgcag gcgatcggcg aggcaatggc gtcggccgag
     1801 ggacaggcga cagcagcgga tctggcgaac ttcgcagacg ccggcgtcga cgtgatgatg
     1861 gcggagacac acctcgtctg agccggcgcg cgacagcgcc atcgcagttc gatgatttcg
     1921 cgacggccgg gtcgacgcgc agcgaaagcg cgaagcgcga gcgcaatgtt ttcgggagaa
     1981 cattttggac gcagaacgac tttcacgccg ctggtatctg gtggcgatca cgtatttcgc
     2041 gatcggcatc gccttcggcg tctacatggg tgcctcgggc aaccattcgc tcttcacggt
     2101 gcacgcgcac atcaatctgc tcggctgggc ctcgatggcg ctgacggggc tgctctatcg
     2161 atcgtttccg gcggcggcgc gaagtcgcct cgccgtctgg cacttctggc tctatcaggc
     2221 ggcggtgccg gtgatgctcg tcgcggtggt cctcatcctt tcgggaacga aggccgccga
     2281 acccgtcgca ggcatcgcct cggtggtgat cctgatatcc gtc
//
LOCUS       DASRWS010000184         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_931977, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000184 DASRWS010000000
VERSION     DASRWS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931977"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2456
                     /gene="dnaE"
                     /locus_tag="VFF72_03340"
     CDS             <1..2456
                     /gene="dnaE"
                     /locus_tag="VFF72_03340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZV92222.1"
                     /translation="ARANGCPVGPGRGSGAGSLVAYSLNITGLDPLRYQLLFERFLNP
                     ERVSMPDFDIDFCQANRDRVIDYVKQKYGRDAVSQIATFGTMAAKAALRDIGRVLGMS
                     YGHVDSVAKLVPAPPGKTVTLRRPPEKPDPGVIYARREAPEIEQRERNEEEVAELLAL
                     AERVEGIVRNVGMHAGGVLIAPGKITDFCPLYMQPGSESAVSQFDKDDVEAIGLVKFD
                     FLGLATLTILELAKELIVARHPDQAGFDYDALPLDDAKVYALFSDGRTESVFQFESAG
                     MQRMLRDARPSRLEDLIALNAMFRPGPMENIPSFCARKHGREEVSYPHPLLEKVLGET
                     YGIMVYQEQVMQAAQVLGGYSLGGADLLRRAMGKKKPEEMATHRTIFRDGAATQKIPA
                     AVADEVFDLMEKFAGYGFNKSHAAAYSLLAYQTAWIKVHYAAEFFAANMTIELDNSDK
                     LKALLVDAKTFGVTIETPDVNAGTYRFEPVSAHAVRFGLGAIKGTGQGAIEAIVAARQ
                     GGGAFRSLFDFCARVDRGRINRRLVEALVKAGAFDSLHASRESLLASVGLALDWADTQ
                     AAHADQGGLFDFGAEEAAHGSSTEEPALVAAAAWSIRERLTLEKSALGFYLSGHLFDQ
                     SAAEVRRFARLKIADAIDSHEPLLLAGIVGDLRIVNGQRGRVAIFKLDDASEAIEAVA
                     REELLDANRDLLKDDELIIVQGKVQPDRFSGGVRLNVQSVWDLVGARCRFGKYLRVEV
                     NGSVPPIAEVLRDFPTRRIPTEHGDLAQGLAVRLALQREGAAGEIDLGEAARFYPSEA
                     ALARWSSAAAHGGATVVYD"
     gene            2476..>2854
                     /locus_tag="VFF72_03345"
     CDS             2476..>2854
                     /locus_tag="VFF72_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZV92223.1"
                     /translation="MTDAALPNGLVLTGGGARAGYQVGVLKAIAQLRREAGAVASGNP
                     FPVITGTSAGAINAAALACRADDFDGAVAGICHVWENFRAEHVYRSDSLGVIRTGARW
                     LTMMSIGWLIARWRRARPRSLLDN"
BASE COUNT          453 a          987 c          976 g          438 t
ORIGIN      
        1 gggctcgcgc caacggctgc ccggtgggcc cggggcgcgg ctcgggtgcc ggctcgctgg
       61 tcgcctattc gctcaacatc acgggcctcg acccgctgcg ctaccagctc ctcttcgagc
      121 gcttcctcaa tcccgaacgg gtctcgatgc ccgacttcga catcgatttc tgccaggcga
      181 accgggaccg cgtcatcgac tacgtcaagc agaagtacgg acgcgacgcg gtgagccaga
      241 tcgccacgtt cggcaccatg gcggcgaagg cggcgctgcg cgacatcggc cgcgtgctcg
      301 gcatgagcta tggccacgtc gactcggtgg ccaagctcgt tcccgcgccg ccgggcaaga
      361 cggtgacgct gcgccggcct cccgagaagc ccgacccggg tgtcatctac gcccgccgcg
      421 aggcgcccga gatcgagcag cgcgagcgaa acgaagagga ggtcgcggag ctccttgcgc
      481 tcgccgagcg cgtcgagggg atcgtccgca atgtcgggat gcacgccggc ggcgtcctga
      541 tcgcgcccgg caagatcacc gacttctgcc cgctctacat gcagcccggc agcgagagcg
      601 ccgtcagcca gttcgacaag gacgacgtcg aggcgatcgg cctggtcaag ttcgacttcc
      661 tgggcctggc gacactgacc atcctcgagc tcgccaagga gctcatcgtc gcccgtcacc
      721 cggatcaggc gggattcgac tacgacgcgc tgccgctcga cgacgccaag gtctacgcgc
      781 tcttctcgga tgggcggacc gagtcggtgt tccagttcga aagcgcgggg atgcagcgga
      841 tgctgcgcga cgcgcggccg agccgcctcg aggacctgat cgcgctcaac gcgatgttcc
      901 ggccggggcc gatggaaaac atcccgagct tttgcgcgcg caagcacggt cgcgaggaag
      961 tgagctatcc gcatccgctg ctggagaagg tgctcggcga gacctacggg atcatggtct
     1021 accaggagca ggtgatgcag gcggcgcagg tgctgggcgg ctactcgctc ggcggcgccg
     1081 atctgctgcg gcgcgcgatg ggcaagaaaa agcccgagga gatggcgacg caccggacga
     1141 tctttcgcga cggcgcggcc acgcagaaga tcccggccgc ggttgccgac gaagtcttcg
     1201 acctgatgga gaagttcgcg ggctacggct tcaacaagtc gcatgctgcc gcctactcgc
     1261 tgctcgccta ccagaccgca tggatcaagg ttcactatgc ggccgagttc ttcgcggcca
     1321 acatgacgat cgagctcgac aacagcgaca agctgaaggc gctgctcgtc gacgcgaaga
     1381 ccttcggcgt gacgatcgaa accccggacg tgaacgccgg cacctaccgg ttcgagccgg
     1441 tgtccgcaca cgcggtccgc ttcggcctcg gcgcgatcaa gggtaccgga cagggcgcga
     1501 tcgaggcgat cgtcgcggct cggcaaggcg gtggtgcctt tcgcagcctg ttcgacttct
     1561 gcgcccgcgt cgatcgcggc cgcatcaacc ggcgcctcgt cgaggccctg gtcaaggccg
     1621 gcgccttcga ttcgctgcac gcctcgcgcg agagcctgct tgccagcgtc ggcctggccc
     1681 tcgactgggc cgatacccag gcggcgcacg ccgaccaggg cggcctcttc gatttcggtg
     1741 ccgaggaagc ggcgcacggc agcagcaccg aggagccggc cctggtcgcg gctgccgcct
     1801 ggagcattcg cgagcggctg acgctggaaa aatcggcgct gggcttctac ctttccggcc
     1861 atctcttcga ccagagcgcc gccgaggtgc gtcgcttcgc gcgcctgaag atcgccgatg
     1921 cgatcgacag ccatgagcct ttgctgctcg ccggcatcgt cggcgacctg cgcatcgtca
     1981 acggccagcg cggccgggtc gccatcttca agctcgacga cgccagcgag gcgatcgagg
     2041 ccgtggccag ggaagagttg ctcgacgcca accgcgacct gctcaaggac gacgaattga
     2101 tcatcgtcca gggcaaggtc cagccggacc gcttttcggg cggcgtccga ctcaacgtgc
     2161 agagcgtctg ggatctcgtc ggcgcgcgct gccgcttcgg caagtacctt cgcgtcgagg
     2221 tcaacggcag cgtgccgccg atcgccgaag tgctgcgcga ctttccgaca cgacgcattc
     2281 ccaccgagca cggcgacctg gcgcagggcc ttgccgtgcg gctggccctg cagcgcgaag
     2341 gcgccgccgg cgagatcgac ctcggcgaag cggcgcggtt ctatccgtcg gaggccgccc
     2401 ttgcgcgctg gagctcggcc gccgcgcacg gcggcgcgac ggtcgtctac gattgaagcg
     2461 aagcgcgcat cgacgatgac cgacgccgca ctgccgaacg ggctcgtcct caccggtggt
     2521 ggcgcccggg ccggctatca ggtcggcgtc ctgaaggcga tcgcgcagtt gcgccgcgag
     2581 gcaggcgccg ttgcttcggg caatccgttc ccggtgatca ccggcacgtc ggccggcgcc
     2641 atcaacgcgg cggcactggc ttgccgcgcc gacgacttcg acggcgccgt cgccggcatc
     2701 tgccacgtct gggagaactt tcgcgccgag cacgtgtacc ggtccgattc gctcggcgtg
     2761 atccgcaccg gcgcgcgctg gctgacgatg atgtcgatcg gctggctcat cgcccgctgg
     2821 cggcgggccc ggccgcggtc gctgctcgac aaca
//
LOCUS       DASRWS010000185         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_932627, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000185 DASRWS010000000
VERSION     DASRWS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932627"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFF72_03350"
     CDS             complement(<1..223)
                     /locus_tag="VFF72_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92224.1"
                     /translation="MDIIAALLDFVVHVDVHLARFVADHGAWVYLLLFAIIFVETGVV
                     VMPFLPGDSLLFVAGAMCAAGLLSLPLVLG"
     gene            complement(255..1571)
                     /locus_tag="VFF72_03355"
     CDS             complement(255..1571)
                     /locus_tag="VFF72_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZV92225.1"
                     /translation="MNDKASNAAGAEESPAFLQAVSDLGRTRQVVTSQPIFNAQGVKL
                     LEGGATVDQGLYDRLVSHRLSRPLDECVEAEPSVDGGVLREAALAAIERWPFFARMAP
                     VGRTREIVLEAIGAASLTKAIGLHLTLARDTRPLLFAHSILMALLCAHLAREGGASLH
                     DITIAATAGLLHDIGMLHIDPELLDSDDRLIGDQLKPVYVHPLTSSMLIGRFAEYPRE
                     IVRAVVEHHEQLDGSGYPRGLSGQAISPLGRMLSLAEVVTAMFDGEREHPEQRVSLLL
                     RINPRRYDAALVPSIHRLLRAAPAPEAGAGGPPERSIARLLRLSDELGRWRETSEQLA
                     SELDGAHAALLQSVSAQNETLQRMLYEAGITREQLGALGDDVGEDSAVGVELWALAEE
                     LLWQLHAAANQLKRRWQATDPALPHPDALAAWLRSVEALDDAAGPG"
     gene            1681..>3060
                     /gene="mutL"
                     /locus_tag="VFF72_03360"
     CDS             1681..>3060
                     /gene="mutL"
                     /locus_tag="VFF72_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZV92226.1"
                     /translation="MNAVLEPRRRRPIRELPGELISQIAAGEVVERPASVVRELLDNA
                     IDAGAATIVVRLTRGGTRAIAVEDDGFGIAAAELPLALRRHATSKIGSLAELEGVATL
                     GFRGEALAAIASVAEVSIASRTGDAPHATRLDARSGELLPAARSEGTSIEVRELFFNT
                     PARRKFLKTETTELAHCLEATRRQAIVRPAIAFAVWHDGKAVARWPVATATERVADVL
                     GAAFIAHSRVVGVDLGGLSIHGRAGTPESARGRADLQYVFVNGRPVRDKLIAHAVRAA
                     YDDVLHGGRHPAYLLFIDVAPERVDVNVHPTKIEVRFRDSREIHEAVRKAVQAALAAP
                     RAGVAPDAAEPEGSPAVASVSGVAAATAQRHLAWPASARWQVAEPPSPWSADAKTGPV
                     TRAAPSTFAHAHRLGEPGAGSTGGGQRAVDAGEGTDDTPWPLGRAIAQIAGTFILAEN
                     AARQLDDVAV"
BASE COUNT          462 a         1110 c         1093 g          395 t
ORIGIN      
        1 ggcccagcac cagcggcagg ctgagcagcc cggcagcgca catcgcaccg gcgacgaaca
       61 gcagcgagtc tcccggcagg aacggcatga cgacgacgcc cgtctcgacg aagatgatgg
      121 cgaagagcag caggtagacc caggcgccgt gatcggcgac gaagcgggcg agatggacgt
      181 cgacgtggac gacgaaatcg agcagcgcgg caatgatgtc catgccgccg attatcgacg
      241 gccgaatcgc tgcctcagcc cgggcctgcc gcgtcgtcga gcgcctcgac gctgcgcagc
      301 cacgcggcaa gcgcgtcggg atgcggcagt gccggatcgg tcgcctgcca gcggcgcttg
      361 agctggttgg ccgccgcatg cagctgccag agcagctctt cggcgagcgc ccagagctcg
      421 acgccgacgg cgctgtcttc gccgacgtcg tcgcccagcg cgccgagctg ctcgcgcgtg
      481 atgccggcct cgtagagcat tcgctgcagc gtctcgtttt gcgcgctcac cgactgcagc
      541 aacgcggcat gcgcgccgtc gagctcgctc gcgagctgct cgctggtctc gcgccagcgg
      601 ccgagctcgt ccgacagccg cagcagccgg gcgatcgagc gctccggcgg tccgcccgcg
      661 ccggcttcgg gcgccggcgc ggcgcgaaga aggcgatgga tcgacggcac gagcgccgcg
      721 tcgtagcgac gcggattgat ccgcaacagc agcgagacgc gctgttcggg gtgttcgcgc
      781 tcgccgtcga acatcgccgt gacgacctcg gccagcgaca gcatgcggcc gagcggactg
      841 atcgcctggc ccgacaggcc acgcggataa ccggagccat cgagctgctc gtggtgctcg
      901 acgacggcac gaacgatctc gcgcgggtat tcggcgaagc gaccgatcag catcgacgac
      961 gtcaacgggt ggacatagac gggcttgagc tggtcgccga tgaggcggtc gtcggaatcg
     1021 agcagctcag ggtcgatgtg cagcatgccg atgtcgtgga gcaggccggc ggtcgccgcg
     1081 atcgtgatgt cgtgcaggct ggcgccgcct tcgcgcgcca gatgcgcgca cagcagtgcc
     1141 atcaggatgc tgtgcgcgaa cagcagcggc cgggtgtccc gcgccagcgt caggtgcagg
     1201 ccgatcgcct tggtcaggct cgccgcgccg atcgcctcga gcacgatctc gcgggtgcgg
     1261 ccaaccggcg ccatgcgggc gaagaaaggc cagcgctcga tggcggcgag agcggcttcg
     1321 cgcagcaccc cgccgtcgac actcggctcg gcctcgacgc actcgtcgag cggccgcgac
     1381 aggcgatgcg agacgagacg gtcgtaaagg ccctggtcga cggtcgcccc gccctcgagc
     1441 agcttcaccc cctgggcgtt gaagatcggc tgcgaggtga ccacctgccg cgtccggccg
     1501 aggtcggaga cggcctgcag gaacgccggc gactcctcgg ccccggccgc gttgctggcc
     1561 ttgtcgttca tcgtccgaag ctaacacgtg acgaaccccg ttcgctgcca ggcgcccgaa
     1621 cacccgccaa ctccgtgacg ccttgccgcc ggacgggatc gccccttcct acaatgcgcc
     1681 atgaatgccg tgctcgagcc acgccggcgg cggccgatcc gcgagttgcc gggcgagctg
     1741 atcagccaga tcgccgccgg cgaggtcgtc gaacgacccg cctcggtggt ccgcgagctg
     1801 ctcgacaacg cgatcgacgc cggcgccgcc accatcgtcg tgcgcctgac gcgaggcggg
     1861 acgcgggcaa tcgccgtcga ggacgacggc ttcggcatcg ccgcggccga gctgccgctg
     1921 gcgctgcgtc gccatgccac cagcaagatc ggctcgctgg ccgagctcga gggcgtcgcc
     1981 acgctcggct ttcgcggcga ggcgctggcg gcgatcgcct ccgtggccga ggtctcgatc
     2041 gccagccgca ccggcgacgc gccgcacgcg acgcgcctcg atgcgcgaag cggcgagctg
     2101 ctgccggcag cgcgcagcga aggcacgagc atcgaggtcc gcgagctgtt cttcaacacg
     2161 ccggcgcggc gcaagttcct gaagacggag acgaccgagc tggcccattg cctcgaggcg
     2221 acgcgccgcc aggcgatcgt ccggccggca atcgccttcg cggtatggca cgacggcaag
     2281 gccgtggccc gctggcccgt ggcgacggcc accgagcggg tcgccgatgt gctgggcgcc
     2341 gccttcatcg cgcacagccg cgtcgtcggc gtcgacctcg gtggcctgtc gatccacggc
     2401 cgtgccggca cgcccgagtc ggcgcgcgga cgcgccgacc tgcagtacgt cttcgtcaac
     2461 ggacggccgg tgcgcgacaa gctcatcgcc cacgccgtgc gcgccgccta cgacgatgtg
     2521 ctgcacgggg gccgccaccc ggcctacctg ctcttcatcg acgtcgcacc cgaacgcgtc
     2581 gacgtcaacg tccatccgac gaagatcgag gtgcgctttc gcgattcgcg cgagatccac
     2641 gaggcggtgc gaaaggcggt ccaggcggca ctggcggcgc cgcgcgccgg ggtcgcgccg
     2701 gatgcagccg aaccggaagg gtcgccggcc gtggcgtcgg tgtccggcgt cgccgcggcc
     2761 accgcgcaac gccacctggc gtggcccgcg agcgcgcgct ggcaggttgc cgagccgcca
     2821 tcgccgtggt cggccgacgc gaagaccggg cccgtcaccc gcgccgcgcc gtcgaccttc
     2881 gctcacgcac atcgtctcgg cgagccgggc gccggctcga ccggcggcgg acagcgggcc
     2941 gtcgatgcgg gcgaaggcac cgacgacacg ccctggccgc tcggtcgggc gatcgcgcag
     3001 atcgccggaa ccttcatcct ggccgagaac gccgcgcgcc aactcgacga cgtcgccgtc
//
LOCUS       DASRWS010000186         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_76078, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000186 DASRWS010000000
VERSION     DASRWS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76078"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFF72_03365"
     CDS             <1..600
                     /locus_tag="VFF72_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZV92227.1"
                     /translation="GSTKRDVVDAARRVLKDRISSFVPAHPIAGKEVSGIAHADASLY
                     HGRQVILTPLPKTSSDLLQKATDAWASIGSQVLRMTPENHDAAFAAVSHLPHILAFAY
                     FNSVSRQPAGRDYLSLGGPGFRDFTRIAASNPEVWRDILMANREEILKQTQRFRHTLD
                     AMEHVVKTGNTEALEALIRSASDARAGWQMNAATPNTHR"
     gene            617..2644
                     /locus_tag="VFF72_03370"
     CDS             617..2644
                     /locus_tag="VFF72_03370"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HZV92228.1"
                     /translation="MYATRFLDLPPLAGVAGTLRVPGSKSISNRALLLAGFAAGTTTL
                     EGLLASDDTRVMIDALAALGAHIEQPGELVVVEGLGGKPLPNDATLFLGNAGTAMRPL
                     TAALAIASAGTGSTYELRGTPRMHERPIADLLAALRGLGCRIDCLEREGYPPLRIQAT
                     SGSLTATGPIHVRGDVSSQFLTALLLALPLRRDAADVTIVVDGELISRPYVDITVSLL
                     ERFGVVVDRQAGDRYLLRGGQRLRSPGRFAIEGDASSATYPIAAAAIAAIDAPLRIDG
                     VGGESIQGDIAFIDAVRAMGARVDVSADHLEVRRGRWPLAAIDLDCNAIPDAAMTLAA
                     LALFADGTSRLRNIASWRVKETDRLAAMASELRKLGAVVNEGADSLAITPPTTWRPAV
                     IDTYDDHRMAMAMSIAAFNGCAGAVPARPVRIVDPRCVGKTWPDYFEALFSMAQADRD
                     SIPVLTIDGPTASGKGTLASAVAQALGYRYLDSGALYRATAIAALEAGVGPDDEAALA
                     ALAGALDLHFDAGRTRLAGRDVTDSLRLEEVGATASKISAWPAVRAALLDLQLAFRRL
                     PGLVADGRDMGSVVFPDATLKVFLTAGAAQRAERRHKQLISKGISANIDSLRADLEAR
                     DARDRNRSASPLEPAEGALLLDNSALSIAASVEVVLAAWEERRPFDPKPSR"
BASE COUNT          409 a         1003 c          891 g          418 t
ORIGIN      
        1 ggctcgacca agcgcgacgt cgtcgacgcg gcgagacggg tgctgaagga ccgcatttcc
       61 tcgttcgtcc ccgcccatcc gatcgccggc aaggaagtgt ccggcatcgc ccatgccgac
      121 gcgtcgctct atcacggccg acaggtgatc ctgacgccgt tgcccaagac atcgagcgac
      181 cttctccaga aggccaccga cgcatgggcg tcgatcggct cgcaggtgct gcggatgacg
      241 ccggagaacc acgacgcggc gttcgccgcc gtgagtcacc tgccgcacat cctcgccttc
      301 gcctatttca attcggtgtc ccgccagccg gccggccggg actacctctc gctcggcggc
      361 ccgggctttc gcgacttcac gcgcatcgcc gccagcaacc cggaagtgtg gcgcgacatc
      421 ctgatggcca accgcgaaga gatcctgaag cagacccagc gcttcaggca caccctcgat
      481 gcgatggagc atgtcgtcaa gaccggcaac accgaggcgc tcgaagcgct gatccgcagc
      541 gccagcgatg cccgtgccgg ctggcagatg aacgccgcga cgccgaacac gcatcgctga
      601 gcgacaggcg gcgccgatgt acgccacgcg cttcctcgac ctcccgccgc tcgcgggcgt
      661 cgccggaacg ctgcgtgtgc cgggatcgaa gagcatctcg aatcgtgccc tgctgctggc
      721 cggattcgcg gcgggaacaa cgaccctcga gggtctgctc gcctcggacg acacccgcgt
      781 catgatcgac gcactcgccg cgctcggcgc gcacatcgag cagcctggcg aactcgtcgt
      841 ggtcgaaggc ctcggcggca agccgctgcc gaacgacgcg acgctgttcc tcggcaatgc
      901 cggcacggcg atgcgaccat tgactgcggc gctcgccatc gccagcgcag gcaccggcag
      961 cacttacgag ctgcgcggca cgccgcgcat gcacgaacgc ccgatcgccg atctgctggc
     1021 ggcgctgcgc ggcctcggct gccggatcga ttgcctggag cgcgaaggct atccgccgct
     1081 gcggatccag gcgacttccg ggagcctgac ggcgaccggc ccgatccacg ttcgcggcga
     1141 cgtatcgagc cagttcctca ccgccttgct gctcgcgctg cccctgcgcc gggacgccgc
     1201 cgacgtgacg atcgtcgtcg acggcgagct catttcccgt ccttacgtcg atatcacggt
     1261 ctcgttgctc gagcgcttcg gcgtcgtcgt cgaccggcaa gcgggagacc gctatctgct
     1321 gcgcggcggc cagcggctgc gctcgcccgg ccgcttcgcc atcgaaggcg acgcctcgtc
     1381 ggccacttac ccgatcgccg cagcggcgat cgccgccatc gacgcgccgc tgcggatcga
     1441 tggcgtcggc ggcgaatcga tccagggcga catcgccttc atcgacgccg tccgggcgat
     1501 gggcgcccgg gtcgacgttt cggccgatca cctggaggtg cgtcgcggtc gatggccgct
     1561 cgccgcgatc gacctcgact gcaacgcgat ccccgatgca gcgatgacgc tggccgcgct
     1621 cgccctgttc gccgacggaa cctcgcggtt gcgcaacatc gccagctggc gagtcaagga
     1681 aaccgaccgc ctggcggcga tggcaagcga gcttcgcaag ctcggcgccg tcgtgaatga
     1741 aggcgccgac agtctcgcca tcacgccgcc gacgacatgg cggccggccg tcatcgacac
     1801 ctacgacgac caccggatgg cgatggcgat gtcgatcgcg gcgttcaacg gctgcgccgg
     1861 cgccgtgccg gctcggcccg tccgcatcgt cgatccgcgc tgcgtcggca agacctggcc
     1921 ggactacttc gaggcgctgt tctcgatggc ccaggccgac cgggattcga tcccggtgct
     1981 caccatcgac gggcccacgg cttcggggaa ggggacgctc gccagcgccg tggcgcaggc
     2041 cctcggctac cgctatctcg actccggcgc cctctaccgc gcgaccgcga ttgccgcgct
     2101 cgaggccggc gtgggtcctg acgacgaggc tgcgctcgcc gccctcgccg gcgccctgga
     2161 cctgcacttc gacgccggac ggacacggct tgccggccgc gatgtcaccg attcgctgcg
     2221 cctcgaggaa gtcggcgcga cggcgtcgaa gatctcggcg tggcccgcgg ttcgcgccgc
     2281 gctgctcgac ctgcaactgg cgttccggcg cttgcccggg ctcgtggccg acgggcgcga
     2341 catgggcagc gtcgtctttc ccgacgcgac gctgaaggtc ttcctcaccg cgggcgccgc
     2401 ccaacgcgcc gagcgccgcc acaagcaatt gatttcaaag ggtatttccg ctaatatcga
     2461 cagtcttcgt gccgacttgg aggcgcgcga cgctcgcgac cgcaatcgca gcgcctcgcc
     2521 tctggagccg gcagaaggtg cgcttctcct cgacaactcg gcactttcga tcgcagcgtc
     2581 ggtcgaagtc gtgctggctg cctgggaaga gcgccggcct ttcgacccga agccgagccg
     2641 ctgagcggcc aggccggtct gaagaccgaa gggcccgccg ctttcccgcc tcgtgccact
     2701 ggcacatcga agcgtcggtt c
//
LOCUS       DASRWS010000187         8380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1792799, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000187 DASRWS010000000
VERSION     DASRWS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792799"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFF72_03375"
     CDS             <1..334
                     /locus_tag="VFF72_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92229.1"
                     /translation="VSTSFQESQLTDEPSMVEVATVELSGQLVGDIGMTLAKMDTELG
                     KATIVNVSCARLIRVDFIAAGDLLNWVLARRSENRTVNFVDAHRMVALFFGAMGINEH
                     ATVKVRTV"
     gene            386..922
                     /gene="hslV"
                     /locus_tag="VFF72_03380"
     CDS             386..922
                     /gene="hslV"
                     /locus_tag="VFF72_03380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZV92230.1"
                     /translation="MDSYHGTTIVSVRRGGLVALGGDGQVTLGNLVVKSTARKVRKLH
                     HDRVLAGFAGATADAFTLFERFEAKLEKHQGHLVRSAIELTKDWRTDRVLRRLEAMLA
                     IADSDTSLIISGNGDVLEPEYGIVAIGSGGSYASAAARALLEHTEMSPRDIVKRSLEI
                     AGDLCIYTNQNHTIETLE"
     gene            1053..2387
                     /gene="hslU"
                     /locus_tag="VFF72_03385"
     CDS             1053..2387
                     /gene="hslU"
                     /locus_tag="VFF72_03385"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZV92231.1"
                     /translation="MKSSMTPQEIVSELDRHIVGQNGAKRAVAIALRNRWRRQQVDES
                     MRGEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDSIVR
                     DLVDMSVKQEREQAMKRQRTRAEDAAEERILEIIVPGARNDLGLAPSPSADNTARQVM
                     RKRLREGALDDKEIEIEVAEAKPSMEIMGPAGMEEMTEQLRGMFANLGGTKKKPKKMR
                     IGDAHKLLIDEEAAKLVNDDDIKASALANAEQNGIVFIDEIDKVASRSEAQGTDVSRQ
                     GVQRDLLPLVEGTTVNTKYGMVKTDHILFIGSGAFHYAKPSDLIPELQGRFPIRVELG
                     SLSVDDFEAILTQTHASLVKQYTLLLATEGVTLELAPEAIRRLAQIAYDVNERTENIG
                     ARRLSTVLERLLDEVSFDAPNRGGQTIRIDAADVDAKLADLAQDVDLSRYIL"
     gene            complement(2416..3189)
                     /locus_tag="VFF72_03390"
     CDS             complement(2416..3189)
                     /locus_tag="VFF72_03390"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZV92232.1"
                     /translation="MAFLAIDIGNTRLKWAMYVEPRPGAAMLGHGATFLEAIDELAEK
                     AWSSVAAPSSMLGSNVAGEGIRRRAEAQLELWDVEPRWVVSSKSACGVSNGYDHPNRL
                     GTDRWVALIGARHRILARGRARPALVVMVGTAVTVDALDAGGLFLGGLILPGFGLMLR
                     ALEMGTAGLKTPTGETVDFPTNTSDALMSGGADAIAGAVDRMYRRLERHTGQAPVLLM
                     SGGAAVKLDTLAHLKVEVVETLIFDGLLQLQAQRLAPSA"
     gene            complement(3195..3773)
                     /locus_tag="VFF72_03395"
     CDS             complement(3195..3773)
                     /locus_tag="VFF72_03395"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HZV92233.1"
                     /translation="MRHARSRWLNPPGRVGDDAAPAALQSFAWRTFMMRQMPGPVEGV
                     VMPGIRRRLVVVAAVWTAAATGLPCVAADLVSAEDGRAIRAVVEGQLAALAADDAKRA
                     FAYAAPAIRQMLGTPERFMAMVRQSYPAVYRPVSTIFLQALREQGQHFQGVQITDADG
                     RLWLATYRLEHEPDGNWRISGCELRRSDAKMT"
     gene            3801..4127
                     /locus_tag="VFF72_03400"
     CDS             3801..4127
                     /locus_tag="VFF72_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92234.1"
                     /translation="MTTRTLVLAAAICSAGLSACVVTPARPYAYESGAVVTDVAPPAP
                     YVETIPVAPFVGAVWIGGYWGWRGNRHVWVPGRYEAPRPGYHWEPHHWVQHGGRWHLE
                     GGAWRR"
     gene            4342..5163
                     /locus_tag="VFF72_03405"
     CDS             4342..5163
                     /locus_tag="VFF72_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HZV92235.1"
                     /translation="MADYRFAGAGGAKSAGRFDLRRFDPSDKPFSSRSKAADKKAVAT
                     LATELDRLQDVFYADRRFKMLVVLQGTDTSGKDGTIRGVFGAMSAQGVQVVPFKVPTD
                     EERSHDFLWRIHQKVPGAGQVVVFNRSHYEDVLVPVVKGNLTARETARRYAQINGFER
                     LLADTGTVVLKFFLHISKEEQRQRLKARLDDPDKHWKFAAGDLDDRKLWDAFQSAYSA
                     AIAATGTPWAPWTVVPADSKTHRNLMIAAAVKEAFEGLGLRYPPGDPALAGIVVT"
     gene            5160..>8380
                     /gene="icmF"
                     /locus_tag="VFF72_03410"
     CDS             5160..>8380
                     /gene="icmF"
                     /locus_tag="VFF72_03410"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HZV92236.1"
                     /translation="MTELAADYKALAAYRPTHKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVVHLGHDRSVDDVVTAAVQEDVQGIAISSYQGGHVEYFKYMIDLLRQRGGAHI
                     RVFGGGGGVIVPAEIAELQAHGVERIYSPEDGQRMGLQGMIGEMLMRCDVDLSPHAPK
                     SMSAIRGTGDGTRRALAQLITALENQAVAPQLRESLRAAARDVRTPVVGITGTGGAGK
                     SSLTDELIRRLRLDQADALHIAVISIDPSRRKSGGALLGDRIRMNAIGPWGTSGSAIA
                     DAAGRRVFMRSLATRDFGSELSAALPDVLAAVKVAGFDLVIVETSGIGQGDAAIVPLV
                     DVPVYVMTPEFGAASQLEKIDMLDFAEFVAINKFDRKGAADALRDVAKQVQRNREAFG
                     QRPDQMPVYGTMASRFNDDGVTALYQAMLPRLSELGLRVETGLLPRVATRHSTHQIPI
                     VPGARARYLADIADTVRGYKRDALAQSRLAREIQQLREAARMLGDANPAKSNAVGAVN
                     GLADERETRLDPQARALLAGWPALQQAYAGDDYVVTVRDREIRTSLVHTTLSGSKIRK
                     VALPRFEDHGEILKWLLLDNVPGSFPYTAGTFAFKRENEDPTRMFAGEGDAFRTNRRF
                     KLLSAGMPAKRLSTAFDSVTLYGNDPAPRPDIYGKVGNSGVSIATLDDMKVLYDGFDL
                     TSPSTSVSMTINGPAPTILAMFMNTAIDQKVAVFRDEKKRDPSVAEAAEIAAWVKQNV
                     RGTVQADILKEDQGQNTCIFSTEFSLKVMGDIAEYFVEHGVRNFYSVSISGYHIAEAG
                     ANPISQLAFTLSNGFTFVEAYLARGMKIDDFAPNLSFFFSNGMDPEYNVMGRVARRIW
                     AVAMRERYGANERSQKLKYHVQTSGRSLHAQEVQFNDIRTTLQALIAIYDNCNSLHTN
                     AFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPTQGAFIIDELTELVEEAVLAEF
                     ERIAERGGVLGAMETGYQRGKIQEESMHYEMQKHSGEYPIVGVNTFRNPHGDPVAGHV
                     ELQRSTDEEKQSQLERLRDFHARHAGESPAMLRRLQDAVIANA"
BASE COUNT         1507 a         2905 c         2727 g         1241 t
ORIGIN      
        1 cgtctcgacg agcttccagg agtcgcagct gaccgacgag ccgtcgatgg tcgaggtggc
       61 gacggtggag ctctcgggcc agcttgtcgg cgacatcggc atgaccctcg ccaagatgga
      121 caccgagctc ggcaaggcga cgatcgtcaa cgtgtcctgc gcccggctga tccgggtcga
      181 cttcatcgct gccggcgacc tcctcaactg ggtgctggcc aggcgcagcg agaaccgcac
      241 cgtcaacttc gtcgatgccc accgaatggt cgccctcttc ttcggtgcga tgggaatcaa
      301 cgagcacgcc accgtcaagg tccgtaccgt ctgatcggcg gattcgcgcc ttgaatcgga
      361 tccgcggcgg cacaatgtgt ccccgatgga cagctatcac ggaacgacga tcgtcagcgt
      421 gcggcgcggc ggtctcgtcg ccctcggcgg cgacggccag gtcacgctcg gcaacctcgt
      481 cgtcaagtcg accgcccgca aggttcgcaa gctccaccac gatcgggtgc tcgccggctt
      541 cgcgggagcc actgccgacg ccttcacgct cttcgagcga ttcgaggcca agctggaaaa
      601 gcaccagggg cacctcgtcc gctcggccat cgagctgacg aaggactggc gcaccgaccg
      661 cgtcctgcgc cgtctcgaag cgatgctggc gatcgccgac agcgacacct ctctgatcat
      721 cagcggcaat ggcgatgtcc tcgagcccga gtacggcatc gtggcgatcg gctccggcgg
      781 gtcgtacgcg agcgccgcgg cgcgcgcgct cctcgagcac accgagatgt cgccgcgcga
      841 catcgtcaag cgctcgctgg aaatcgccgg cgacctgtgc atctacacca accagaacca
      901 cacgatcgag acgctcgaat gaagcgatgc ctgcacgcac acggggacac ccgcggtctc
      961 gcgaggcggc agcgcccggc cccgggcggc ccgatggccg gccagcggcg ccttgacgcc
     1021 tcctgcgtcc ggccagcggc gacggtgcgg ccatgaaatc gagcatgacc ccgcaggaga
     1081 tcgtctccga gctcgaccgg cacatcgtcg gccagaacgg cgccaagcgc gccgtcgcca
     1141 tcgccttgcg caatcgctgg cgtcgacagc aggtcgacga gtcgatgcgc ggcgagatca
     1201 cgccgaagaa catcctcatg atcgggccga ccggcgtcgg caagaccgag atcgcgcggc
     1261 gcctggcgcg cttggcggac gcgccgttca tcaaggtcga ggcgaccaag ttcaccgagg
     1321 tcggctatgt cggcaaggac gtcgactcga tcgtccgcga tctggtcgac atgtcggtca
     1381 agcaggagcg cgaacaggcg atgaagcgcc agcgcacccg cgccgaggat gccgccgaag
     1441 agcggatcct cgagatcatc gttcccggcg cgcgcaacga tctcggcctg gcgccctcgc
     1501 cctcggccga caacaccgcg cgccaggtca tgagaaagcg cctgcgcgaa ggcgcgctcg
     1561 acgacaagga gatcgagatc gaagtcgccg aggcgaagcc gtcgatggaa atcatggggc
     1621 cggccgggat ggaagagatg accgagcaat tgcgcggcat gttcgccaac ctcggcggca
     1681 cgaagaagaa gccgaagaag atgcgcatcg gcgatgcgca caagctgctg atcgacgaag
     1741 aggcggccaa gctcgtcaac gacgacgaca tcaaggccag cgcgctggcc aacgccgagc
     1801 agaacggcat cgtcttcatc gacgagatcg acaaggtggc ttcgcgatcg gaagcgcaag
     1861 gcaccgatgt gtcgcgccag ggggtgcagc gcgacctgtt gccgctggtc gagggcacga
     1921 ccgtcaacac caagtacggc atggtcaaga ccgaccacat cctcttcatc ggctcgggcg
     1981 cgttccacta cgccaaaccg agcgacctga tcccagaatt gcagggccgc tttccgatcc
     2041 gggtcgagct cggctcgctg tcggtcgacg atttcgaggc catcctgacg cagacgcatg
     2101 ccagcctcgt caagcagtac accctgcttc tggcgaccga gggcgtaacc ctcgagctcg
     2161 ctcccgaggc gatccgccgg ctggcccaga tcgcctacga cgtcaacgag cgcaccgaga
     2221 acatcggcgc acgccgcctg tcgacggtcc tcgaacggct gctcgacgag gtgagcttcg
     2281 atgcacccaa tcgcggcggc cagacgattc gcatcgacgc cgccgatgtc gatgcgaaac
     2341 tggccgacct ggcccaggac gtcgacctct cgcgctacat cctctgaccg gggggaacct
     2401 gcgtggcgcg gcgggtcagg ccgagggcgc caggcgctgg gcctgcaact gcagcagccc
     2461 gtcgaagatc agcgtctcga cgacctcgac cttgaggtgg gcgagcgtgt cgagcttgac
     2521 ggcggcgccg ccgctcatca ggagcacggg ggcttgcccg gtgtgtcttt cgagccgtcg
     2581 atacatccgg tcgacggcac cggcaatcgc gtcggcgccg ccgctcatca acgcgtcgct
     2641 ggtgttggtc gggaaatcga ccgtttcgcc ggtcggcgtc ttgaggccgg ccgtgcccat
     2701 ttccagggct ctcagcatga gaccgaagcc gggcaggatg aggccaccca ggaacagtcc
     2761 cccggcatcg agcgcgtcga cggtgacggc cgtcccgacc atgacgacga gcgccggcct
     2821 ggcccggccg cgcgcgagga tgcgatgccg cgcgccgatc agcgccaccc agcggtcggt
     2881 gccgagccga ttcggatggt cgtagccgtt gctgacgccg catgccgact tcgaactcac
     2941 cacccagcgt ggctcgacgt cccacagctc gagctgggcc tcggcacgac gccggatgcc
     3001 ttctccggca acgttgctgc cgagcatgct ggacggcgca gccaccgacg accaggcctt
     3061 ctcggcgagt tcatcgatcg cctcgagaaa cgtcgcaccg tggcccagca tcgccgcgcc
     3121 gggccgtggc tcgacgtaca tggcccactt cagccgcgtg tttccgatat cgatggccaa
     3181 aaacgccatg gccgtcaggt catcttcgcg tcgcttcgcc gaagctcgca gccgctgatc
     3241 cgccagttgc cgtccggctc gtgctcgagc cgatacgtcg ccagccacag ccggccgtcg
     3301 gcatccgtga tctgcacgcc ctggaaatgc tggccctgct cgcgcagcgc ctgcaggaag
     3361 atcgtcgaaa ccggtcggta cacggccggg taactctgac ggaccatcgc catgaaccgc
     3421 tcaggcgttc ccagcatctg ccgaatcgca ggcgccgcgt aggcgaacgc acgctttgca
     3481 tcgtcggccg ccaaggcggc aagctgccct tcgacgaccg cgcgaatcgc acgcccgtcc
     3541 tcggcggaca cgaggtcggc cgcgacgcag ggcaggccag tggcggcagc cgtccagacg
     3601 gcggccacga ccacgagtcg gcgacggatt ccaggcatga caactccttc gacgggaccg
     3661 ggcatttgcc gcatcatgaa cgtacgccag gcaaaggact gtaaagcggc cggcgcggcg
     3721 tcatcgccga cccgtcccgg cgggttaagc catcgtgatc gggcatggcg catgcccttt
     3781 gccttctcag gaggattcgc atgaccactc gcacactcgt cctcgccgcc gcgatctgca
     3841 gcgccggcct ctcggcctgc gtcgtcaccc cggcacgccc ctacgcctac gaatccggcg
     3901 ccgtcgtcac cgacgtcgct ccgccggcgc cgtacgtcga gaccatcccg gtggcgccgt
     3961 tcgtcggcgc ggtgtggatc ggcggctact ggggttggcg gggcaatcgc cacgtctggg
     4021 ttcccggacg ctacgaggcg ccgcgccctg gctatcactg ggagccgcac cactgggtcc
     4081 agcacggcgg ccgctggcat ctcgaaggcg gtgcctggcg tcgctgagcg gcaccgcgcc
     4141 tggacgcttg ccgtcgcgac ggtcccggcg ctgctgatcg cggaaagact cgccccggcg
     4201 gtcagcacgg gggcgtccgc catggtgcgg gctacacttc gagttgacgt atacgtcaac
     4261 tgaccggaat cgcccggtcg agtccgaagt agcggagacc ccttggcgaa aagcagcccg
     4321 aacggccacg agcaagcccg tctggcggac tatcgattcg ccggcgccgg cggggcgaag
     4381 tccgccggtc gcttcgacct tcgccggttc gacccatcgg acaagccgtt ttcgagtcgc
     4441 agcaaggcgg ccgacaagaa ggccgtcgcc acactggcga ccgagctcga ccgcctgcaa
     4501 gacgtcttct acgccgatcg tcgattcaag atgctggtgg tgttgcaagg caccgacacc
     4561 agtggcaagg acggcacgat ccgcggcgtc ttcggggcca tgagcgcgca aggcgttcag
     4621 gtcgtcccgt tcaaggtacc gaccgacgaa gagcgctcgc acgactttct ctggcgcatc
     4681 caccagaagg ttccaggcgc aggccaggtg gtcgtcttca accgcagcca ctacgaagac
     4741 gtcctggtgc cggtcgtgaa aggcaatctc actgcccggg agacggcgcg gcgctacgcg
     4801 cagatcaacg gcttcgagcg gcttctcgcc gacaccggca cggtggtgct caaattcttc
     4861 ctgcacatct cgaaggaaga gcagcgccag cgcctgaagg cgcgtctcga cgatcctgac
     4921 aagcactgga aattcgccgc cggcgatctg gacgaccgca agctgtggga cgcattccag
     4981 tcggcctaca gcgcggcgat cgcggccacc ggcacgccct gggcgccgtg gacagtcgtg
     5041 cccgccgatt cgaagacgca tcgaaatttg atgatcgccg ccgccgtgaa ggaggccttc
     5101 gagggcctcg gcctgcgcta cccgccgggt gatccggcgc ttgccggaat cgtcgtcaca
     5161 tgaccgaact cgctgccgac tacaaggcgc tggctgccta tcgcccgacc cacaaggtcc
     5221 gcttcgtcac cgccgcgagc ctgttcgacg gccacgacgc ggcgatcaac atcatgcgcc
     5281 gcatcctgca gggcatgggc gcggaagtcg tgcaccttgg ccacgaccgc tcggtcgatg
     5341 atgtggtgac ggcggcggtt caggaagacg tgcaaggcat cgccatcagc tcctaccagg
     5401 gcggccacgt cgagtacttc aagtacatga tcgatctgct gcgccagcgt ggcggagccc
     5461 acatccgggt cttcggcggc ggcggcggcg tgatcgtgcc ggcggagatt gccgagttgc
     5521 aggcgcatgg cgtcgagcgc atctacagcc ccgaggacgg ccagcgaatg ggcctgcaag
     5581 ggatgatcgg cgaaatgctg atgcgctgcg acgtcgactt gtcgccgcac gcgccgaagt
     5641 cgatgtcggc catccgcggg accggcgacg ggaccaggcg agcgctcgcg cagctgatca
     5701 cggcgctgga gaaccaggcc gtcgccccgc aacttcgcga gtcgctgcgc gcggcagcgc
     5761 gcgacgtgcg cacaccggtc gtcggcatca ccggtaccgg gggcgccggc aagtccagcc
     5821 tcaccgacga gctgatccgg cgcctgcgcc tcgaccaggc cgacgcactg cacatcgccg
     5881 tcatctcgat cgacccgtcg cgacgcaaga gcgggggcgc gctgctcggc gaccgcatcc
     5941 gcatgaacgc gatcggtccc tggggcacat cgggaagcgc gatcgccgac gccgccggac
     6001 ggcgcgtctt catgcgctcg ctcgccaccc gcgacttcgg ctccgaactc agcgccgcgc
     6061 tgcccgatgt gctcgccgcc gtgaaggtgg cggggttcga cctcgtgatc gtcgagacct
     6121 cgggcatcgg ccagggcgac gcggcgatcg tgccgctggt cgacgtcccg gtctacgtga
     6181 tgacgcccga gttcggcgcc gccagccagc tcgagaagat cgacatgctc gacttcgccg
     6241 agttcgtcgc catcaacaag ttcgaccgca agggtgcggc cgatgcgctg cgcgacgtcg
     6301 ccaagcaggt gcagagaaac cgcgaggcgt tcggccagcg gcccgaccag atgcctgtct
     6361 acggcacgat ggccagccgc ttcaacgacg acggcgtcac ggcgctgtac caggccatgc
     6421 tgccgcgact ttccgagctc ggcctgcgcg tcgagacggg gctgctgccg cgggtcgcga
     6481 cgcgacacag cacgcaccag atcccgatcg tgccgggcgc ccgggcccgc tacctggccg
     6541 acatcgccga caccgttcgc ggctacaagc gcgacgccct ggcgcaatcc cggctggcac
     6601 gcgagatcca gcagttgcgc gaggccgccc ggatgctggg cgacgccaac ccggcaaaga
     6661 gcaacgccgt cggcgccgtc aacggcctgg ccgacgaacg tgagacgcgc ctggacccgc
     6721 aagctcgcgc cctgctggct ggctggcccg cgctgcagca ggcctatgcc ggcgatgact
     6781 atgtcgtcac cgtacgcgac cgcgaaatcc gtacttccct cgtccacacg acgctgtcgg
     6841 gcagcaagat caggaaggtg gcgctgccgc gcttcgagga ccatggcgag atcctgaagt
     6901 ggcttctcct cgacaacgtt ccgggctcgt tcccgtacac cgcggggact ttcgcattca
     6961 agcgcgagaa cgaggatccg acccggatgt tcgccggcga aggcgacgct ttcagaacca
     7021 acaggcggtt caagctgctt tccgccggca tgccggccaa gcggctgtcg accgctttcg
     7081 attccgtcac gctctacggc aacgatccgg cgccgcggcc cgacatctac ggcaaggtcg
     7141 gcaactcggg cgtctcgatc gccacgctcg acgacatgaa ggtgctctac gacggcttcg
     7201 acctgaccag cccgagcacc tccgtgagca tgacgatcaa cggccccgcg ccgacgatcc
     7261 tggcgatgtt catgaacacc gccatcgacc agaaggtcgc ggtatttcgc gacgaaaaga
     7321 agcgcgatcc gagcgtcgcc gaggcagcgg aaatcgccgc ctgggtcaag cagaacgtgc
     7381 gcggcaccgt gcaggccgac atcctgaagg aagaccaggg ccagaacacc tgcatcttct
     7441 cgaccgagtt ctcgctgaag gtgatgggcg acatcgccga gtacttcgtc gagcacggtg
     7501 ttcgcaactt ctactcggtg agcatctcgg gctatcacat cgccgaggcc ggcgccaacc
     7561 cgatcagcca gctcgccttc acgctgtcga acggcttcac cttcgtcgag gcctacctcg
     7621 cgcgcggcat gaagatcgac gacttcgcgc cgaacctgtc gttcttcttc tccaacggca
     7681 tggacccgga atacaacgtc atgggccggg tcgcgcggcg catctgggcg gtggcgatgc
     7741 gtgagcgcta cggcgccaac gagcgctcgc aaaagctcaa gtaccacgtg cagacgagcg
     7801 gccgctcgct gcacgcccag gaggtgcagt tcaacgacat ccgcaccacc ttgcaggcgc
     7861 tgatcgccat ctacgacaac tgcaactcgc tgcacaccaa cgccttcgac gaggcgatca
     7921 cgacgccgac cgaagacagc gtgcgccggg cgatggcgat ccagctcatc atcaaccgcg
     7981 agtggggcct ggcgaagaac gagaacccga cgcagggcgc cttcatcatc gacgagctga
     8041 ccgagctggt cgaggaggcg gtgcttgccg agttcgagag gatcgccgag cgcggcggcg
     8101 tcctcggcgc gatggaaacg ggctatcagc gcggcaagat ccaggaagag tcgatgcact
     8161 acgagatgca gaagcactcg ggcgaatatc cgatcgtggg cgtcaatacc ttccgcaacc
     8221 cgcacggcga tccggtcgcc ggccacgtcg agctgcagcg ctccaccgat gaagaaaagc
     8281 agagccagct cgagcggctg cgcgacttcc atgcgcgcca tgccggcgag tcgccagcga
     8341 tgctgcgacg gctccaggac gcggtgatcg ccaacgccaa
//
LOCUS       DASRWS010000188         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3247328, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000188 DASRWS010000000
VERSION     DASRWS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247328"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFF72_03415"
     CDS             complement(<1..943)
                     /locus_tag="VFF72_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92237.1"
                     /translation="MKRIVASAPGKLVLTGEYAVLEGASAVVLAVDRRARVVLDDHDG
                     DDYRIEAPDIGVAVAHCRIEAAGRVRWVDVDGDRAARLALVAAVLQAAAQNGAPAPFR
                     VGLDTREFVAADGVGLQAKLGLGSSAAIAVALSGAIHVREGRQPPSPRSLIDAHRRFQ
                     GGRGSGLDVAASLSGGATIYRRRRGEPQITPAAWLGEIGLCCVWTGRAASTRAMLEHM
                     AAWRAREPALCASLMEELAAVAAIAADALRAGNARALLDAVRDYAGGLDRLGRASGVD
                     IVCAEHRAIGAIAAAHGVTYKSCGAGGGDVGVALAIDA"
     gene            complement(940..1932)
                     /gene="mvaD"
                     /locus_tag="VFF72_03420"
     CDS             complement(940..1932)
                     /gene="mvaD"
                     /locus_tag="VFF72_03420"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HZV92238.1"
                     /translation="MTAHAQAHPNIALIKYWGKRDDRLNLPATGSLSITLESMSARTR
                     VAFDRGLDDDVFRLDGEANPAALARVVACLDLLRARAGTTWRARVDSSNDFPAGAGLA
                     SSASGFAALVLAASTALDLRLDRRELSVLARRASGSAARSMFGGFVAMSAGSRADGDD
                     ACAEPLLDAGEWPLEVVIAVTSHGAKAIGSSTGMDISRRSSPFHGEWIAGSAADLDEA
                     RRAVLGRDFAALAAVSEHNGLKMHAVMLASRPALVYWSGATLDCLHCIRALREREGLE
                     VFFTSDAGAQAIAVCRPAAAARVAASLSQIPGVHRLLRSALGEGAHLVADIEAA"
     gene            complement(1989..3023)
                     /gene="hpnA"
                     /locus_tag="VFF72_03425"
     CDS             complement(1989..3023)
                     /gene="hpnA"
                     /locus_tag="VFF72_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HZV92239.1"
                     /translation="MTDVVLLTGATGFVGSAVARSLLAAGFHVRALVRATSRLDNLRG
                     LDVERVVGDVRTPQTLLAACTGCRGVFHVAADYRLWAPDPATIYASNVAGSTNVLRAA
                     AQAGVGRIVYTSSVATLGLHADGTPADEDTPVLLSEMAGDYKRSKFLAEQCVREIAAK
                     DGLDVVIVNPAAPVGPRDIRPTPTGRMILDAARGRMPAYVDTGLDIVHVDDVAAGHLL
                     AYERGVAGRRYVLGGENLTLRDILGRVARLSGRREPWLRLPHAAVLPMAWLAERWATV
                     GKGEPLVTLAGARLARHRMFFSHARAERELGYRARPAQQGIADALAWFEANGDLHLHE
                     HRGAAVGTGS"
     gene            complement(3020..3712)
                     /locus_tag="VFF72_03430"
     CDS             complement(3020..3712)
                     /locus_tag="VFF72_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZV92240.1"
                     /translation="MSVRSGRVALAGVGGERAAAAAARLIDAGARALLSWGVAGALAP
                     RLEPGDLLLPSKVVTARRSWFVDAAWREAILEGLGSAHGGNLWCSAVPVGDVAQKRDL
                     ADRGMVAVDMESAAVAAVAARRGLPFIAIKSICDPASRAVPAVATDLLGIDGRLHLAT
                     LARALRAGPRAWRELNRLRIDHAAACRSLRDAARTLPALGPRPAAPTRWPEPGCGQGQ
                     AGFGDVGRSAAQ"
BASE COUNT          574 a         1447 c         1425 g          492 t
ORIGIN      
        1 cggcatcgat ggcgagcgcg acgccgacgt cgccgcctcc ggcgccgcaa ctcttgtacg
       61 tgacgccgtg tgccgcagcg atggcgccga tcgcgcgatg ctcggcgcag acgatgtcga
      121 cgccgctcgc gcgaccgagc ctgtccagcc ccccggcgta atcgcgcacg gcatcgagca
      181 aggcccttgc gttgccggcg cgcagcgcgt cggcggcgat cgcggcgacc gctgcgagct
      241 cttccatcaa cgaagcgcaa agcgcaggct cgcgcgcacg ccacgccgcc atgtgttcga
      301 gcatggcccg ggtcgaggcc gcgcggccgg tccagacaca gcaaaggccg atctcgccga
      361 gccacgctgc gggcgtgatc tgcggctcgc cgcgccgacg ccgatagatc gtcgcgccgc
      421 cgctcaggct ggcggcgacg tccaggccgc tgccgcgtcc gccctggaag cgacggtggg
      481 cgtcgatcag gctgcgcggc gaaggcggct gtcggccctc gcgtacgtgg atcgctcccg
      541 acagggcgac cgcgatcgcc gcgctcgagc cgagcccgag cttggcctga aggccgacgc
      601 cgtcggcggc aacgaattcg cgcgtgtcga gcccgacgcg aaagggcgcc ggggcgccgt
      661 tctgggccgc tgcctggagc accgcggcga ccagcgccag tctcgccgcg cggtcgccat
      721 cgacatcgac ccagcggacc cggccggccg cttcgatgcg gcagtgcgcg acggcgacgc
      781 cgatgtcggg cgcctcgatc cggtagtcgt cgccgtcgtg gtcatcgagc acgacccgag
      841 cgcgacggtc gaccgccagc acgacggcgc tcgcgccttc gagcaccgcg tactcgccgg
      901 tgaggaccag cttgcccggc gccgacgcga cgatccgctt cacgccgcct cgatgtcggc
      961 gacgagatgc gcgccttctc cgagcgcgct cctcagcagc cgatgaacgc ccgggatctg
     1021 cgagagcgac gccgcgacgc gagctgccgc ggcgggccgg cacacggcga tggcctgcgc
     1081 cccagcgtcg ctcgtgaaga agacctccag gccctcgcgc tcgcgcaacg cccggatgca
     1141 atgcaggcag tcgagcgtcg ctccggacca gtacaccagc gccggccggc tcgccagcat
     1201 caccgcgtgc atcttcaggc cgttgtgttc gctgaccgct gcgagcgccg cgaaatcgcg
     1261 cccgaggacc gcgcggcgcg cttcgtccag gtcggcggcc gagccggcaa tccactcgcc
     1321 atggaagggc gagctgcgcc ggctgatgtc cattccggtg ctcgagccga tcgccttcgc
     1381 gccatgcgag gtgaccgcga tgaccacctc gagcggccat tcgccggcgt cgaggagtgg
     1441 ctcggcacag gcgtcgtcgc cgtcggcgcg gctgcccgcg gacatcgcca cgaagccgcc
     1501 gaacatcgag cgcgccgccg aaccggaagc acgccgcgcc agcaccgaca gctcgcgtcg
     1561 atcgagcctc aggtcgagcg cagtgctcgc ggcgaggacc agggctgcga aaccggaagc
     1621 cgaggacgcg agcccggcgc cggccgggaa gtcgttcgag gaatcgacgc gggcgcgcca
     1681 ggtcgtgccg gctcgcgcgc gcagcaggtc gaggcacgcg acgacgcgtg cgagcgccgc
     1741 cgggttcgcc tcgccgtcga gacgaaagac atcgtcgtcg agcccgcgat cgaatgcgac
     1801 acgcgtgcgt gcggacatcg attcgagcgt gatcgaaaga gagccggtcg ccggaaggtt
     1861 gagccggtcg tcgcgcttgc cccagtactt gatcagcgcg atgttggggt gcgcctgcgc
     1921 gtgagccgtc atgatcccgg tcgccgctgc agccgggcgc acggcaattc ggccgggcgc
     1981 accgcggctc atgatccggt tccgaccgcc gcgccgcggt gctcgtgcag gtgcaggtcg
     2041 ccgttggcct cgaaccacgc cagcgcatcg gcgatgccct gctgcgccgg ccgcgcccgg
     2101 tagccgagct cccgttccgc acgcgcatgc gagaagaaca tcctgtggcg cgccaggcgg
     2161 gccccggcca gggtgacgag cggctcgccc ttgcccaccg tcgcccagcg ctcggcgagc
     2221 cacgccatcg gcagcacggc ggcgtgcggc agccgcagcc acggctcgcg acggccgctg
     2281 agtcgggcga cccggccgag gatgtcgcgc agcgtcaggt tctcgccacc gaggacgtag
     2341 cgtcggccgg cgacgccgcg ctcgtaggcc aggaggtggc ccgcggcaac gtcgtcgacg
     2401 tgcacgatgt cgagaccggt gtcgacgtac gccggcatgc ggccgcgcgc cgcgtcgagg
     2461 atcatccttc cggtcggcgt cggcctgatg tcgcgcggac cgaccggcgc tgccgggttg
     2521 acgatcacga cgtccaggcc atccttcgcg gcgatttcgc gcacgcattg ctcggcgagg
     2581 aatttcgagc gcttgtagtc gcccgccatc tccgagagca gcaccggcgt gtcttcatcg
     2641 gcgggcgtgc catcggcatg aagtccgaga gtcgcgacac tgctcgtata gacgatgcgt
     2701 ccgacacccg cctgcgcggc tgcgcgcagg acgttcgtgc tgccggcgac attgctggcg
     2761 tagatcgtcg ccgggtcggg tgcccacagc cgatagtcgg cggcgacgtg gaagacgccg
     2821 cggcagcccg tgcaggcagc aagcaaggtc tgcggcgtgc gcacgtcgcc gacgacgcgc
     2881 tcgacgtcga gcccgcgcag gttgtcgagc cggctcgtcg cccgcaccag ggcgcggaca
     2941 tggaagccgg ccgccagcag gctgcgcgcg acggccgagc cgacgaagcc ggtggcgccg
     3001 gtcagcagga cgacatcggt cattgcgccg cgctcctgcc gacgtcgccg aaccccgcct
     3061 ggccttgccc gcaaccgggt tcgggccagc gcgtcggtgc ggcaggccgc gggcccaacg
     3121 cgggcaatgt ccgggccgcg tcgcgcagcg agcgacacgc ggcggcatgg tcgatgcgca
     3181 ggcggttcaa ttcgcgccag gcgcgcgggc ccgcgcgaag ggctcgcgcc agggtcgcca
     3241 gatgcagccg gccgtcgatg cccagcagat ccgtcgcgac tgccggaacg gcccgtgacg
     3301 ccgggtcgca tatcgacttg atcgcgatga agggcaggcc gcgacgcgcg gcgaccgccg
     3361 cgaccgcggc gctttccatg tcgacggcga ccatgccgcg gtcggcgagg tcgcgcttct
     3421 gcgcgacgtc gccgaccggc acggcactgc accagagatt gccgccgtgg gcgctgccga
     3481 ggccctcgag gatcgcctcg cgccaggccg catcgacgaa ccagctgcgc ctcgccgtga
     3541 ccaccttcga gggcagcagc aggtcgcccg gctcgagccg cggcgcgagg gcgcctgcga
     3601 cgccccagct cagaagcgct cgcgcaccgg catcgatcag cctggcggcg gcagcagcgg
     3661 cgcgctcgcc accgactccc gccaacgcga cgcggccgct tcgcacgctc atggagctgc
     3721 ccgggcggca ggatccggcg cccagcgcct gcgcctccgc ggtcatggcg acgacgatgc
     3781 ccagatcagg gcacgaggcc gacgcgcgca ttgcgatagc gggcgagcgc ccagtacgga
     3841 aagtagaggc tgtagccgtg gtacttcaga tagaagacgc gcggaaagcc cggggcgttc
     3901 gcgccgccgt catgccagcg gccctggcgc tgcagatg
//
LOCUS       DASRWS010000189         6395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1517715, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000189 DASRWS010000000
VERSION     DASRWS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517715"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1064
                     /locus_tag="VFF72_03435"
     CDS             303..1064
                     /locus_tag="VFF72_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV92241.1"
                     /translation="MSSHLFIVTGASRGMGAAIAGQLLDRPDTVLLGISRRDNPGLAE
                     RARASGSTVLQWPTDLADPLAAAARLRAWLDAQDGIGFATASLVNNAAALTRIGALED
                     CNAEELSRAVRVGLEAPLLLMAAFLGATAKWPGRRRVLNISSGLGRRAMAGQASYCAT
                     KAGLDHLSRAAALDQAQQTRGATVVSLAPGVIDTDMQSELRAARGDGFPERDNFVRLK
                     EDGGLASPDEAAARVIAWLERADFGTSPVADVRDA"
     gene            1395..2393
                     /locus_tag="VFF72_03440"
     CDS             1395..2393
                     /locus_tag="VFF72_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZV92242.1"
                     /translation="MFGFAKASSATRPAPGLLAHAAQGGWLKREEAIMGTAISAELWS
                     DDRAAGEAAIDAVMAEMHRIDAAMSPYKEDSELSRINRDASDGRVPVSAEMAMLLDRA
                     NRFSALSDGAFDITYAAVGHLYDYRRGIRPTDSELAHAREAVGWRHLIVDVAARTVRF
                     GRPGMRIDLGGFAKGHAVDNATAILRDLGIAHANVSAGGDSRVIGDRRGRPWTIGVRD
                     PRRPGEMVAVLPLEDVSISTSGDYERYFDEAGVRHHHLIDPSTGRSPSGVHSVTVLAE
                     DGLTTEAMSKTVFVLGVEKGLRLVESMPGIDAIVVAANGALHYSSGLLAPAQRAHQ"
     gene            2433..5429
                     /locus_tag="VFF72_03445"
     CDS             2433..5429
                     /locus_tag="VFF72_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92243.1"
                     /translation="MNRQLILGAWGCASLALLAGCSGGSTSDSSTLTVQGDVPIVYAQ
                     RVNTINANPTSVGGSAPGGDIMIREKSSPSAAEHNITAQFTQGNGDARDPDVSFDGKK
                     IVFSMKCPTSNTSMIGTVPACTGQWNIWEYDMTTGGLTGGTFRRLTNGGSNDVEPSYL
                     PAGKGFVFTSDRQTTSKTQQINGHTYFALDEYEREQVFNLHTMDAQGGNITQISVNQS
                     HDRNPTVRPTGDIMFSRWDHVGGRNHFKIFTVKPDGTNMFVLYGAHSPGNSFLNPRDM
                     DPAGPHAGFLSSDLMPLSRTHEGGGLVFIDAAHYSEDGTPATAGVPTSGGQHQATTQA
                     LNIGEGLSQYGRITTPYPLWDGTDRVLASFTPCEVNRKGVGTVSCSTLSAAEMARLSQ
                     TERLTTDIAKDDLQDNVPPSYSVYMFDPKAQTFLIVAAPPAGKMNVHPAPILARTEPN
                     ATEPLAGDPALAAQNLGLLDVRSVYDTDGLGRMGDAVLTAADLPAGCTTAIAKTAPTD
                     PEDTRAQIADLVKMKDPAQPAYNCAPARFIRAVRAVAPMQGMTGMRSAIGETEFEMQQ
                     ILGYAPIEPDGSFKLTVPANTPIGLAVVNADGLAFQTHTNWIQVRPGERRTCDGCHSP
                     RRGAAINSGATVNSQPASLLPAMASAHLSGETYADTRTRLDPSKLLLASDMIYTDVWA
                     DTSKAGVTGRSAIALKYTGNANPADDLATLAPVNGIINYPEHIQPLWTRDRGANTCTN
                     CHNDPDLLDLGGTIAGDGRVESYDEVMIGDPVLDPVTGLPETEIKEGVLVIKRGPALV
                     STMASEGDALGLARQSRLAEILWGETLMAGTDALAAHPNPPATAPDHSKMLNAAEKRL
                     LAEWMDTGGKYYNDPFNPNSGIRQIKALDEDSFTANVLPILTKTCAAYCHQAIGSDDP
                     TLPAQGTSFRSNRFVLTGDPDGDYGVTLSMISNACNPASNYLLSKPSSVPHPAGATGA
                     SASTTAVLPVGSADYNTIAAWIAGGC"
     gene            5528..6076
                     /locus_tag="VFF72_03450"
     CDS             5528..6076
                     /locus_tag="VFF72_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92244.1"
                     /translation="MASCGGGGTNPLDNPQDVSNPAVTSGQHLAFAYFQRCVEPILFS
                     QISNGTSINSCAGAGCHAEKTGTGGAFRLELDATPLDVTDPANTADVIRASDIYKNFY
                     SAQGEVVIGDPIQSKLFQKPLVLTVNHGGGKIFTSDTDPNATIFKYWITHPAPDGQDE
                     FSPATYSMFTPPDPNTGTCNTQ"
     gene            6073..>6395
                     /locus_tag="VFF72_03455"
     CDS             6073..>6395
                     /locus_tag="VFF72_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92245.1"
                     /translation="MKHRAFAWAAVLIAGALALSRADAADAAPPVASSPAATRSDAAS
                     PAVTSTSTASTRAPVSASGNEMLQVADPYLEMRTGPGRGYPVFFVVQRNDWIEIELRH
                     TDWFRV"
BASE COUNT         1112 a         2325 c         2005 g          953 t
ORIGIN      
        1 agccacgaac ccgggttctc gatcgctgcc tggctccaga ggtacccgct gaaccgcggc
       61 ccgtaccggc agcgactgtt gcagctgacg tccggcgtgc tgggctagcg tcccgccgag
      121 atcggcattg ctggcgatca cgtgccgacg gccgtcgcca tcgcggcaca cgatgggcga
      181 gcagctgaat cgagtgtcgt cgcctcgccg cgcagcgaac cgccgaggcg tcggcccgtc
      241 gcggcggggc ctcttatgat cggcgccctt gtcgcgacga tcgcttcgcg ggagcgccat
      301 ccatgtcaag ccacctcttc atcgtcaccg gcgcctcgcg cggcatgggc gctgccatcg
      361 ccggccaact gctcgatcgc cccgacaccg tcctgctcgg catctcgcgt cgcgacaatc
      421 cgggcctcgc cgaacgcgcc cgggcaagtg gctcgacggt gcttcaatgg ccgacggatc
      481 tggccgatcc cctggccgcc gcggcgcgtc tgcgcgcgtg gctcgatgcg caggacggca
      541 tcggcttcgc caccgcaagc ctcgtcaaca acgccgcggc actgactcgc atcggtgcgc
      601 tcgaggattg caacgccgaa gagctgtcgc gagccgtgcg cgtcggcctc gaagcaccgc
      661 tcctgttgat ggccgccttt ctcggcgcga ccgcgaagtg gcccggacgt cgccgcgtcc
      721 tgaacatctc ctcgggcctc ggtcgtcggg cgatggccgg acaggccagc tactgcgcga
      781 cgaaggcggg cctcgatcac ctgtcgcgcg ccgccgcgct cgatcaggca caacagaccc
      841 gcggggcgac ggtcgtgtcg ctggcgcctg gcgtcatcga caccgacatg cagtccgagc
      901 tgcgcgccgc acgaggcgat ggctttcccg agcgcgacaa cttcgtgcgc ctgaaggagg
      961 atggcgggct ggcatcgccc gacgaagcgg cggctcgcgt gatcgcctgg ctcgagcgcg
     1021 cggatttcgg aacgtcgccg gtggccgacg ttcgcgacgc ctgagccctc ggcaccttcg
     1081 gtcgacggcc gaagggcgtg tcacgcgagc cgggtctcga agccatgcgc atcgcgcgca
     1141 ccgcctcgca gcgccgattc cggtcggcga caaatccgtt gcgattcgtc acatctgaag
     1201 cgtctgaacg caaggacgtc gaaacgcgtc ccttcccact cccgccggca aggtctgaaa
     1261 gccattgcta acaaatattg acgcccttgc gaggtcgaac cgtggtcaag tctcgggcac
     1321 gtgatctgcg tgtcccccgg ggcgccgcaa gcgccaccga accgccaacc gacccgagcc
     1381 ttccacacgc caccatgttc ggcttcgcca aggcatcctc cgcaacccgg ccggcacccg
     1441 ggctgctcgc ccatgcggcg cagggcggct ggctgaagcg cgaagaagcc atcatgggaa
     1501 cggcgatcag tgccgagctc tggagcgatg accgggctgc cggcgaggcg gccatcgacg
     1561 cggtcatggc cgagatgcac cgcatcgacg cagcgatgag cccgtacaag gaagactcgg
     1621 agctgtcgcg catcaaccgc gacgcgtcgg acggccgggt tccggtgagc gcggagatgg
     1681 ccatgctgct ggatcgcgcc aaccgcttct cggcgctttc cgacggcgca ttcgacatca
     1741 cctacgcggc cgtcggccat ctctacgact accggcgcgg catccggccc accgactccg
     1801 aactggctca tgcgcgcgaa gcggtaggct ggcgacacct gatcgtcgac gtcgccgcgc
     1861 gcaccgtgcg cttcggtcgc cccggaatgc gcatcgacct cggcggcttc gccaagggtc
     1921 atgcggtcga caacgccacg gcgatccttc gcgatctcgg catcgcccat gccaatgtca
     1981 gcgccggcgg cgacagccgg gtcatcggcg accggcgcgg ccgcccctgg acgatcggcg
     2041 ttcgcgatcc gcggcggccc ggcgagatgg tcgcggtgct gccgctggaa gacgtgtcga
     2101 tctcgacgtc gggcgactac gaacgctatt tcgacgaagc gggcgtgcgc caccatcacc
     2161 tgatcgaccc ctcgaccggg cgctcgccgt cgggcgtgca cagcgtgacg gtgctcgccg
     2221 aagacggcct cacgaccgag gcgatgtcga agacggtgtt cgtgctcggg gtcgagaagg
     2281 gcctgcgcct ggtcgagtcg atgccgggca tcgatgcgat cgtcgtcgcc gcgaacggcg
     2341 cactgcacta ctcatccggc ctgctcgcac ctgcgcagcg ggcccatcaa tgacagttgg
     2401 cgtttgatca agaaaactct ttggaggtca ccatgaacag gcaactgatt ctgggcgcgt
     2461 ggggatgcgc ttctctggct ctgctggccg gctgcagcgg cggcagcacc agcgactcga
     2521 gcacgttgac ggtgcagggc gacgtgccca tcgtctatgc gcagcgcgtc aacacgatca
     2581 atgccaaccc gacctcggtc ggcggctcgg cgccgggtgg cgacatcatg atccgcgaga
     2641 aatcctcgcc gagcgccgcg gagcacaaca tcaccgccca gttcacccag ggcaatggcg
     2701 acgcgcgcga ccccgacgtg tcgttcgacg gcaagaagat cgtcttctcg atgaagtgcc
     2761 cgacctcgaa cacctcgatg atcggcaccg ttccggcctg caccggccaa tggaacatct
     2821 gggaatacga catgacgacg ggcggcctga cgggcggcac gttccgccgc ctcaccaacg
     2881 gcggcagcaa cgatgtcgag ccgagctacc tgccggcagg caagggattc gtcttcacgt
     2941 cggatcgcca gacgacctcg aagacgcagc agatcaacgg ccacacctac ttcgccctcg
     3001 acgaatacga gcgcgaacag gtattcaacc tgcacacgat ggacgcccag ggcggcaaca
     3061 tcacccagat ctcggtcaac cagagccacg atcgcaaccc gaccgtgcgc ccgaccggcg
     3121 acatcatgtt ctcgcgctgg gaccacgtcg gcggccgcaa ccacttcaag atcttcaccg
     3181 tcaagccgga cggcaccaac atgttcgtgc tgtacggcgc gcacagcccg ggcaacagct
     3241 tcctgaaccc gcgcgacatg gatccggccg gcccgcacgc aggcttcctg agctccgacc
     3301 tgatgcctct gtcgcgcacc catgagggcg gcggcctggt cttcatcgac gccgcgcact
     3361 attcagagga cggcacgccg gcgacggcgg gcgttccgac cagcggcggc cagcatcagg
     3421 cgaccaccca ggcgctcaac atcggcgaag gcctgtcgca atacggccgc atcacgacgc
     3481 cctatccgct gtgggacggc accgatcgcg tcctggcttc gttcacgccc tgcgaagtga
     3541 atcgcaaggg tgtcggcacc gtctcgtgct cgacgctgtc ggcggccgag atggcccgcc
     3601 tctctcagac cgagcggctg acgaccgaca tcgccaagga cgacctgcag gacaacgtgc
     3661 cgccgtcgta ctcggtctac atgttcgacc ccaaggcgca gaccttcctg atcgtcgccg
     3721 cgccgccggc cggcaagatg aacgtgcacc cggcaccgat cctggcgcgc accgagccga
     3781 acgccaccga acccctggcc ggcgaccccg ccctcgccgc gcagaacctc ggcctgctcg
     3841 acgtgcgcag cgtctacgac accgacggcc tcggccggat gggtgacgcc gtcctcacgg
     3901 cggccgacct gccggccggc tgcacgacgg cgatcgccaa gacggcgccg accgaccccg
     3961 aggacacgcg tgcccagatc gccgacctgg tgaagatgaa ggatccggcg cagccggcct
     4021 acaactgcgc cccggcacgg ttcattcgcg ccgtgcgcgc cgtcgcaccg atgcaaggca
     4081 tgaccggcat gcgcagcgcg atcggcgaga ccgagttcga gatgcagcag atcctcggct
     4141 atgcgccgat cgagcccgac ggctcattca agctgaccgt gccggccaac acgccgatcg
     4201 gcctggcggt cgtcaatgcc gatggcctcg ccttccagac gcacaccaac tggatccagg
     4261 ttcgtcccgg cgaacgtcgc acctgcgacg gctgccacag cccgcgtcgc ggcgccgcaa
     4321 tcaactccgg cgccaccgtc aacagccagc cggcgtcgtt gctgccggcg atggcctcgg
     4381 cgcatctctc gggcgagacc tacgccgaca cgcggacccg cctcgatccc agcaagctgc
     4441 tgctggccag cgacatgatc tacaccgatg tctgggccga taccagcaag gccggcgtca
     4501 ccggacgctc ggcaatcgcg ctgaaataca caggaaacgc gaatccggcg gacgatctcg
     4561 cgacgctcgc accggtgaat ggcatcatca attatccgga gcacatccag cccctgtgga
     4621 cgcgagatcg tggcgccaat acctgcacca actgccacaa cgaccccgac ctgctcgatc
     4681 tcggcggcac gattgccggc gatggtcgcg tcgaatcgta cgacgaggtc atgatcggcg
     4741 acccggtcct cgatccggtc accggcctgc cggaaacgga gatcaaggaa ggcgtgctgg
     4801 tcatcaagcg cggcccggcc ctggtcagca cgatggccag cgaaggcgat gcgctcggcc
     4861 ttgcgcggca aagccgtctc gctgaaatcc tctggggcga gacgctgatg gccggcaccg
     4921 acgcactggc ggcgcacccg aatccgcccg ccaccgcgcc cgaccattcg aagatgctca
     4981 acgccgccga gaagcgcctg cttgccgagt ggatggacac cggcggcaag tactacaacg
     5041 acccgttcaa tccgaacagc ggcatccgcc agatcaaggc cctcgatgaa gactcgttca
     5101 cggccaacgt cctgccgatc ctgacgaaga cctgcgccgc ctattgccac caggcgatcg
     5161 gcagcgacga tccgacgctg ccggcacagg gaacgtcgtt ccgcagcaac cgcttcgtgc
     5221 tgaccggcga cccggacggc gactacggcg tgacgctgtc gatgatctcg aacgcctgca
     5281 atccggcgtc gaactacctg ctgtcgaagc cttcgtcggt gccccacccg gccggcgcaa
     5341 cgggcgcgtc ggcctcgacg acggcggtgt tgccggtcgg cagcgccgac tacaacacca
     5401 tcgccgcctg gatcgcggga ggttgctgaa caagatgatc cggtctcacc cacgcgacga
     5461 cgttccaccg tctcttctcc ttcgctctgc acgacggtgt gtcatcgcgt ccgccgcggc
     5521 tctcctgatg gcgagctgcg gcgggggtgg aaccaacccg ctcgacaatc cgcaggacgt
     5581 ctccaatcct gccgtcacgt cgggccagca tctggccttc gcgtacttcc agcggtgcgt
     5641 cgagccgatc ctgttctccc agatctcgaa cggcacgtcg atcaactcgt gcgccggtgc
     5701 cggctgtcac gccgagaaga cgggcaccgg cggcgccttt cgcctcgagc tcgacgccac
     5761 ccccctcgac gtgaccgacc cggcgaacac cgctgacgtc attcgcgcga gcgacatcta
     5821 caagaacttc tattcggcgc aaggcgaggt cgtcatcggc gacccgatcc agagcaagct
     5881 gttccagaag ccgctcgtcc tcaccgtcaa tcatggtggc ggcaagatct tcacgagcga
     5941 caccgacccc aacgcgacga tcttcaagta ctggatcacg cacccggctc ccgatggcca
     6001 ggacgaattc agtcccgcga cctattcgat gttcactcct cccgacccga acaccggcac
     6061 gtgcaatacc caatgaagca ccgcgccttc gcgtgggcag cggtgctgat cgccggcgcg
     6121 ctcgcactga gccgcgccga tgccgccgat gcggcgcctc ccgttgcctc gagccccgcc
     6181 gcgacgcgca gcgacgccgc gtcgcccgcc gtcacgtcga cgagcaccgc ctcgacgcgg
     6241 gctccggtgt cggcgtcggg caacgagatg ctgcaggtcg ccgatccgta cctcgagatg
     6301 cggaccggcc cggggcgagg ctacccggtc ttcttcgtcg tccagcgcaa cgactggatc
     6361 gagatcgagc tgcgccacac cgactggttc cgtgt
//
LOCUS       DASRWS010000190         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2368088, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000190 DASRWS010000000
VERSION     DASRWS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368088"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFF72_03460"
     CDS             <1..515
                     /locus_tag="VFF72_03460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV92246.1"
                     /translation="ADPAGTASRPVPQALRDLFERFESGQSMLEVRVGAWRELEAAAK
                     SIRSAVFVGEQNIPAAMEWDDADADAVHAVAFNGLGRALATGRMLEHVPGVAKIGRMA
                     VLSDSRHGGVGRTVLEALLDAARKRGDREAVLHAQLGAAPFYEKVGFMRRGPEFDEAG
                     IAHVEMLRVL"
     gene            complement(522..827)
                     /locus_tag="VFF72_03465"
     CDS             complement(522..827)
                     /locus_tag="VFF72_03465"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HZV92247.1"
                     /translation="MRELFVYYRVDAASVRRARQAVDAMQGALRSAHPGLQTRLLERI
                     AVDGRITWMETYAFPAAARPAGVDAAIEKDIVERARPLAPFFDGDRHVEAFHACGEG"
     gene            complement(847..1788)
                     /locus_tag="VFF72_03470"
     CDS             complement(847..1788)
                     /locus_tag="VFF72_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZV92248.1"
                     /translation="MNSRPELTGACRLDDWGVIRARGADAATFLQGQLTNDVLGLDDA
                     AARLAGFCSAKGRLQASFVVWRTQAEEFLLACSADLLAPTLKRMSMFVLRARCQLVDA
                     SAEVTLRGLAGATASEHLRDVAVWHRRDAGEAQVIRLPDGAGHPRAILAAPSSGDATS
                     TAAPRLDVDVWRWLAVQSGIVTIEAATVDRFVPQMVNFERIGGVDFKKGCYPGQEVVA
                     RSQYRGTAKRRTFLFDCEAIPAAGQDVFATGAPQEAMGTVANAAPRPGESGGSALVEL
                     RLAALHQGELRLGAVDGPRLDRRELPYPLPAEPELAA"
     gene            1860..2858
                     /gene="mltG"
                     /locus_tag="VFF72_03475"
     CDS             1860..2858
                     /gene="mltG"
                     /locus_tag="VFF72_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZV92249.1"
                     /translation="MRWVRIALALALVGVALMAAASWWLHRPLVLASDPLELSIEPGS
                     SPREIAEAWVRAGVRSEPAALYEWFRWSGQARRIRAGSYQIGAGTTPIDLLEKMVRGE
                     QTQDYIRFTEGWTFRQIRDQLAHAASLKHTIDGLSDEDVMRELGAPGQSPEGRFHPDT
                     YAYSKGSTDLAVLKRAYRAMQVRLAAAWQERSADTPLHNPDEALTLASIVEKETGVEA
                     DRGLVAGVFVNRLRLGMPLQTDPTIIFGLGTAFDGSLHKRDLQTDSPYNTYLHAGLPP
                     TPISIPGRAALLAAVRPEATKALYFVARGDGSSEFSATLTEHNRAVNRYQRKPASP"
     gene            2855..3469
                     /gene="tmk"
                     /locus_tag="VFF72_03480"
     CDS             2855..3469
                     /gene="tmk"
                     /locus_tag="VFF72_03480"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZV92250.1"
                     /translation="MTGRFITFEGIDGAGKSTHIQPAAARCRDAGRRVTVTREPGGTD
                     LAETLREAILHTSMDGIVETLLVFAARRDHIDRVIAPALGRGDVVLCDRFTDATYAYQ
                     GFGRGQDLALLQHLEDAVQEGLQPDLTLWFDVDPRIAAKRRAQARAADRFEAEDLAFF
                     ERVRGGYLARMNAAPRRVVRIDASRDRLDVERQVGAAIDAQIDA"
     gene            3462..4502
                     /gene="holB"
                     /locus_tag="VFF72_03485"
     CDS             3462..4502
                     /gene="holB"
                     /locus_tag="VFF72_03485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZV92251.1"
                     /translation="MPEPQRAVLPWLDAPLRDALEGSNAHALLIHGSADVGQLEFALA
                     LARGWLCESTTAPMGSRPCGQCASCRLVAAGSHPDLLVLVPEAMRETAGAAGGGDDDG
                     EEKSSRKKPSKEIRVDEVRVAVAFALTTSARGRGKVVVVHPAERMNAIAANAFLKTLE
                     EPAGDARFLLCSAAPDALLPTIRSRCQALRLRLPTLGESLAWMREQGVEGAEVLLAAS
                     GGQPREAVARAAAGIDAASWKGLPARVAAGDSSAFRGWPLPLAIDALQKLCHDAACVA
                     AGAAPRFFPADTLSRVGSLPSLLHYARELSRFARHADHPWIADLGVESLVTQGREALH
                     TPRSSRRGPRVQ"
BASE COUNT          719 a         1580 c         1617 g          692 t
ORIGIN      
        1 tcgccgatcc ggcaggcacc gcgtcgcgac cggtgccgca ggcgctgcgc gacctcttcg
       61 aacgcttcga gtcgggccag tcgatgctcg aggtccgggt cggtgcctgg cgcgagctcg
      121 aagcggccgc gaagtcgatc cgcagcgcgg tgttcgtcgg cgaacagaac attcccgccg
      181 ccatggagtg ggacgacgcc gacgccgacg cggtgcatgc ggtcgccttc aacgggctgg
      241 ggcgcgcgct ggcgacgggg cgcatgctcg aacacgtgcc gggcgtcgcc aagatcggcc
      301 ggatggccgt cttgtcggat tcccgtcatg gcggcgtcgg ccggacggtc ctcgaagcgc
      361 tgctcgatgc cgctcgcaag cgaggcgatc gcgaagcagt gctccacgcg cagctcggcg
      421 cggcgccttt ttacgagaag gtcggattca tgcgacgcgg tcccgagttc gacgaggcgg
      481 gcatcgccca cgtcgagatg cttcgagtcc tctgaggcgg ctcagccctc gccgcaggcg
      541 tggaatgcct cgacgtgacg gtcgccgtcg aagaagggcg ccagcgggcg cgctcgttcg
      601 acgatgtcct tctcgatcgc cgcgtcgact ccggctggcc gcgccgcagc cgggaaggca
      661 tacgtttcca tccaggtgat gcgcccgtcg acagcgatcc gctcgagcag cctcgtctgg
      721 agcccgggat gcgccgatcg tagcgcgccc tgcattgcgt cgaccgcctg ccgagcgcgg
      781 cgcaccgacg cggcgtcgac ccggtagtag acgaacagtt cgcgcacgcg aaaaggcgaa
      841 tccggctcag gccgccagct cgggttcggc gggaagcgga taaggcaact cgcgtcggtc
      901 cagccgcgga ccgtcgaccg cgcccagacg cagctcgcct tgatggagcg ctgccaggcg
      961 aagctcgaca agggcgcttc cgccggactc tccgggccgg ggcgcggcgt tggctaccgt
     1021 tcccatggct tcctgtggcg cgccggtggc gaagacgtcc tgtccggccg cgggtatcgc
     1081 ctcgcagtcg aagagaaagg tccgtcgctt cgccgtgccg cggtattggc ttcgcgcgac
     1141 gacttcctgg ccggggtagc agcccttctt gaaatcgacg ccgccgatcc gctcgaagtt
     1201 caccatctgc ggcacgaacc ggtcaacggt cgccgcctcg atcgtcacga tgccgctctg
     1261 caccgcgagc cagcgccaga cgtcgacgtc gaggcgcggc gcggcggtgg acgttgcatc
     1321 gcccgacgac ggagcggcga ggatcgctcg cgggtgaccg gcgccatcgg gcaggcgaat
     1381 gacctgcgcc tcgcctgcat cgcgacggtg ccagaccgcg acgtcgcgca ggtgctccga
     1441 tgcggtggcc ccggcaaggc cccggagcgt cacctcggcg ctggcatcga cgagctggca
     1501 gcgggcgcga aggacgaaca tcgacatccg cttcaacgta ggcgccagga gatcggccga
     1561 gcacgcgaga agaaattcct cggcctgcgt ccgccagacg acgaagctgg cctgcagtcg
     1621 acccttcgcc gaacagaagc cggcgagtcg tgccgccgcg tcgtcgaggc cgaggacatc
     1681 gttcgtcaat tggccctgaa ggaaggtcgc cgcatcggcg ccgcgggccc ggatcacgcc
     1741 ccaatcgtcg agccggcaag cacccgtgag ctcaggtcgg ctgttcatgc gccattatca
     1801 tcggcagcca tgcgggatgt cgaagcgagg tcgcaagcgc gcgggttcgt cgcgggccga
     1861 tgaggtgggt ccggatcgcg ctcgcgctgg cgctcgtcgg ggtcgcgttg atggccgccg
     1921 catcgtggtg gctgcaccgg ccgctcgtgc tcgcgagcga tccgctcgag ctgtcgatcg
     1981 agccgggcag ctcgccacgc gaaatcgccg aagcatgggt gagggcggga gtgcggtcgg
     2041 agccggccgc gctgtacgaa tggttccgat ggtccgggca ggcgcgtcgg atccgcgccg
     2101 gcagctatca gatcggtgcc ggaacgacgc ccatcgactt gctcgagaag atggtgcgag
     2161 gcgaacagac gcaagactac atccggttca cggaagggtg gactttccgc cagatacgcg
     2221 atcagctggc gcatgcggct tctctcaaac acaccatcga cgggctcagc gacgaggatg
     2281 tcatgcgcga gctcggcgcg ccgggccagt cgccggaagg gcgctttcac cccgacacct
     2341 atgcctacag caagggctcg acggatctgg cggtgctcaa gcgggcctat cgggcgatgc
     2401 aggtgcgtct tgcggcggcc tggcaggaac gttcagccga cacgccgctg cacaacccgg
     2461 acgaggcgct gacgctcgcg tcgatcgtcg agaaggagac cggcgtcgaa gccgatcgcg
     2521 gactggtcgc cggcgtcttc gtgaacaggc tgcgcctggg aatgccgctg cagaccgatc
     2581 cgacaatcat cttcggcctc ggcaccgcgt tcgacggcag cctgcacaag cgtgatctgc
     2641 aaaccgacag tccctacaac acgtacctgc acgccgggct gccgccgacg cccatctcca
     2701 ttcctggccg ggcggccttg ctcgccgcgg tgcggcccga ggcgacgaaa gcgctctatt
     2761 tcgtcgcccg cggcgacggc agcagcgaat tcagcgccac gctgaccgag cacaatcgcg
     2821 cggtcaatcg ctaccagcgc aaacccgctt caccgtgacc ggccgcttca tcaccttcga
     2881 aggcatcgat ggcgcgggca agtcgacgca tatccagcca gccgcagcac gttgccgtga
     2941 cgcaggtcgt cgggtgacgg tgacccgcga gcccggcggg acggacctcg ccgagacatt
     3001 gcgcgaggcg atcctgcaca cgtcgatgga cgggatcgtc gagacgctgc tggtcttcgc
     3061 ggcgcgccgc gaccatatcg accgcgtgat cgcgccggcc ctcgggaggg gcgacgtcgt
     3121 cctgtgcgac cggttcaccg atgcgacgta cgcgtaccag ggcttcggcc gcggccagga
     3181 cctggcgctc ctccagcacc tcgaagacgc ggtgcaggaa ggcctgcagc ccgatctgac
     3241 gctgtggttc gacgtcgatc cgcggattgc ggcaaagcgc cgggcccagg cccgtgccgc
     3301 cgatcgtttc gaagccgaag acctggcgtt cttcgagcgg gtgcgcggtg gctacctcgc
     3361 acgcatgaac gccgcgccgc gacgcgtcgt gcggatcgac gcgtcgcgcg atcgcctcga
     3421 cgtcgagcgg caagtcgggg ccgcgatcga cgcgcaaatc gatgcctgaa ccgcaacggg
     3481 cggtgctgcc ctggctcgat gcgccgctgc gcgatgcgct cgaaggctcg aacgcgcatg
     3541 ccctgctgat ccacggatcg gccgatgtgg gtcaactcga gttcgccctc gccctggcgc
     3601 gaggctggct gtgcgagtcg acgaccgcac cgatgggcag ccggccctgc ggccagtgcg
     3661 cgagctgccg gctcgtagcg gccggttcgc accccgatct gctggtgctc gttccggaag
     3721 cgatgcgcga aacggccggc gcggccggcg gcggtgacga cgatggcgag gagaagtcgt
     3781 cgcgcaaaaa gccgagcaag gagatccgtg tcgacgaagt acgcgtcgcc gtggccttcg
     3841 cactcaccac gtcggcgcgg ggcaggggca aggtggtcgt cgtccatcct gccgagcgga
     3901 tgaacgccat tgccgccaac gcattcctga agacgctgga agagccggcc ggcgatgccc
     3961 gcttcctcct ctgcagcgcc gcgcctgacg cgttgttgcc gacgatacga agccgctgcc
     4021 aggcgctgcg cctgcgactg ccgacgctcg gggagtcgct tgcctggatg cgcgaacagg
     4081 gcgtcgaagg tgccgaggtg ctgcttgcgg cttcgggtgg gcagcctcgg gaagcagtgg
     4141 cgcgggcagc cgcaggcatc gacgccgcgt cgtggaaagg cctgcccgcc cgcgtcgccg
     4201 cgggggattc atcggccttt cgtggctggc cgctgccgtt ggccatcgac gccttgcaaa
     4261 agctctgcca cgacgccgcc tgcgtggctg caggcgcggc tccgcgcttc ttcccggccg
     4321 acacgctgtc gcgcgtcgga tcgttgccgt cgctgctgca ctatgcgcgc gagctgagcc
     4381 gattcgcccg ccatgccgac cacccctgga tcgccgacct cggggtcgag agcctcgtca
     4441 cccaggggcg cgaagcgttg catacgccgc gttcatcgcg gcgtggtccg agagtgcagt
     4501 agatacactc gcgcgcatgg ccgaacctgc agcaactcgg ggcacgacgc ccggaatcgt
     4561 cgcgccagca ccggccgccg cccggccgag catcatccag ctcgtgtt
//
LOCUS       DASRWS010000191         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_938238, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000191 DASRWS010000000
VERSION     DASRWS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938238"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFF72_03490"
     CDS             complement(<1..596)
                     /locus_tag="VFF72_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92252.1"
                     /translation="MLTSLFGDLGPARDGKKKSRDFDDTQADDNSGFAATAIMESVAT
                     EVNERGQIIDRHVSDLVVVGSPAQAIREHFAATRADLETASSMITLLDPVGVWASSVI
                     KALSDAGGRPIEKLHLREHTTLRTIAMIERTMLVRRNEDTLRIFHADVRAPGPENAEI
                     PVALMERSQMTAVIIGPMQPHAIDALLASLRHATSLAT"
     gene            complement(801..1544)
                     /locus_tag="VFF72_03495"
     CDS             complement(801..1544)
                     /locus_tag="VFF72_03495"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HZV92253.1"
                     /translation="MKFSRRQGLVIVGALASLMACSSLLAADGIVEGSGRMTSVTRPA
                     AGVARLAVAGPFSVEVRQGSHPGVTLEGDDNLLALVETRIDGPTDRATLHIAPIPGVT
                     LEPTQPIRVRIEVAALAAIDLAGDGRVFAFDARSDRLDVAIGGGGRVELPRLEAKVVA
                     VRIGGSGAIRADGRAGNLSVRIGGSGRCDADRLIVDDATVSIGGSGRADVDATERLVV
                     SIAGSGRVRQHGAAVPQVSVVGSGRVDRL"
     gene            complement(1727..>2350)
                     /locus_tag="VFF72_03500"
     CDS             complement(1727..>2350)
                     /locus_tag="VFF72_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZV92254.1"
                     /translation="NRRAIDEARTLGAPCLVLVVGGLPGAFTGSPNSKDLAAARRDVQ
                     DGIATTLEYARGVGMPLAIEPLHPMYAADRACVNTLEQALDLCDALDPASSRDAPRST
                     AALGVAVDVYHVWWDPRLEAQIARCGRDRLLAFHVCDWLVPTRDLLNDRGMMGDGVID
                     LRRIRGWVEAAGFTGYSEVEIFSTQNWWKRSADDVLSTCIERHRRVV"
BASE COUNT          348 a          802 c          834 g          366 t
ORIGIN      
        1 cacgtcgcca ggctggtggc atggcgcagc gacgcgagca gcgcgtcgat cgcgtgcggc
       61 tgcatcggcc cgatgatcac cgcggtcatc tggctgcgct ccatcagcgc gaccgggatc
      121 tcggcgttct cggggcccgg cgcgcggacg tcggcatgga agatacgaag cgtgtcctcg
      181 ttgcggcgga cgagcatcgt ccgctcgatc atcgcgatcg tgcgcagtgt cgtgtgctcg
      241 cgcaggtgca gcttctcgat cgggcggccg cccgcatcgg agagcgcctt gatcaccgac
      301 gaggcccaga cgccgaccgg gtcgagcagc gtgatcatcg agctggcggt ctcgagatcg
      361 gcgcgggtcg ccgcgaaatg ctcgcggatc gcttgcgccg gcgagccgac gacgacgagg
      421 tcgctgacat gacggtcgat gatctggccg cgctcgttga cctcggtggc gacgctttcc
      481 atgatcgcgg tcgcggcgaa accgctgttg tcgtcggcct gggtgtcgtc gaagtcgcgc
      541 gatttcttct tgccgtcgcg ggcgggtccg aggtctccga acagcgaggt cagcatgagc
      601 gttcctgtgc gccgcgcgat ggcggctctc cgatcgatcg gagtctaggc cggggctgcc
      661 aacgctgtct ttgtactttt ggttacaacg atgcacggct cgacgaggcc gatggccggc
      721 gcacgctcga ccgcgtcgtc tggcgccgtg ctcgtccgcc tggttggccc tgtgagccgg
      781 ggtagccgtt gccgtgggcc tcagaggcga tcgacgcgtc cgctgccgac gacgctcacc
      841 tgcggcaccg ccgcgccatg ctggcggacg cgccccgatc ctgcgatcga gacgaccagg
      901 cgctcggtcg cgtcgacgtc ggcccggccg ctgccgccga tcgagacggt cgcgtcgtcg
      961 acgatcaagc gatccgcatc gcaacggccg ctgccgccga tgcgcacgga gagattgccg
     1021 gcgcgtccat cggcgcgaat ggcgccgctg ccgccgatcc tgacggcgac caccttcgct
     1081 tccagccggg gcaactcgac ccggccgccg ccgccgatcg cgacgtcgag tcgatcggat
     1141 cgagcgtcga aggcaaagac gcgtccgtct cccgcgagat cgatggcggc aagggcggca
     1201 acttcgatgc gtactcggat cggttgagtg ggttcgagcg tgacgccggg gatcggcgcg
     1261 atatgcaggg tcgctcgatc cgtgggcccg tcgatgcggg tctcgacgag cgccagcagg
     1321 ttgtcgtcgc cttcgagcgt cacgcccgga tgcgagccct gtcgcacttc gacgctgaac
     1381 gggccggcca ccgccagcct ggcgacgccc gcggcgggac gcgtgacgct cgtcatccgg
     1441 cccgagcctt cgacgatgcc atcggcagcc aggagcgatg aacacgccat cagcgacgcc
     1501 aaggcgccga cgatgaccag accttgcctg cgggagaact tcatcctggt atccgaagcg
     1561 gaggaagact cgatgctgcc gcagccttca cgcccgagcc aggtcgatcc gacgggctgc
     1621 cgttcgcgcc gacgtgcggc acgagcaggc gacagtcggt cggtgggcgc cgcgaaaatg
     1681 acggaaggag ccgggtcgcg atccgcggcc gctggctctt cgctactcac acgacgcgcc
     1741 gatgccgttc gatgcaggtg ctcaacacgt cgtcggcgga gcgtttccac cagttctggg
     1801 tcgagaagat ctcgacctcg ctgtagccgg tgaaacccgc cgcctcgacc cagccgcgga
     1861 tgcgcctcag gtcgatgacg ccgtcgccca tcatgccgcg gtcgttgagc aggtcgcgcg
     1921 tcggcaccag ccagtcgcag acatggaagg ccagcagcct gtcgcgaccg cagcgggcga
     1981 tctgggcctc gagcctggga tcccaccaga cgtgatagac gtcgacggcc acgccgagcg
     2041 ccgccgtcga tcgcggcgcg tcgcggctcg atgccggatc gagcgcgtcg cacaggtcga
     2101 gcgcctgctc cagggtgttg acgcaggcac gatcggcggc gtacatcgga tgcagcggct
     2161 cgatggccaa cggcatgccg acgccgcgtg cgtattcgag cgtcgtggcg atgccatcct
     2221 gcacgtcgcg ccgcgccgcc gccaggtcct tcgaattcgg cgagccggtg aacgcgcccg
     2281 gcaggccgcc gacgacgagc acgaggcagg gcgcgccgag cgtgcgcgcc tcgtcgatgg
     2341 cgcggcggtt
//
LOCUS       DASRWS010000192         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_369058, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000192 DASRWS010000000
VERSION     DASRWS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369058"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFF72_03505"
     CDS             <1..194
                     /locus_tag="VFF72_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZV92255.1"
                     /translation="KLADLRYRNGASSYLDSLDAQRALFVAQQALIQVQAAQLQNTVA
                     LYRALGGGWKDEAATTATR"
     gene            complement(225..2423)
                     /locus_tag="VFF72_03510"
     CDS             complement(225..2423)
                     /locus_tag="VFF72_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZV92256.1"
                     /translation="MTLNDGEGRPLEDDATARVSRRTFFRWSSGWAIGAGPLGASIGL
                     AACGGGDSSPEPSSSLPPSAPAPGPTILENPATASISIQARQVQWVVGKTPANANAWV
                     YVADGSAPASGVLGNSLGPFFNVRRDSACTVTWTNTIGASPLVPARLADPPINVPLDL
                     GMCGRVVNQSPVGLAAHLHGARVQADADGWPLTPVGFAGNPYGFPQSRQDFYPNAQRG
                     TMLWYHDHGMDRVGRHVHAGLCGPYCIRDAADDAILALIGGRSQELLFVMTDRILNAD
                     QTAIDYDAGIPHDYSLSNIGSDDAVGRPEFLGDQIFINGHPAPELTLARGAWRLRVLD
                     LSNARTYALALCDPDAIAAQTGRVWYTQCMRLIGGDGGLMGRSVALQDTDALVIAPAQ
                     RRDVVVDLSAVPATVTRLRLVNISLRYLLAVDELTLEAIYTTFDDSVLAPTNARFDEA
                     DQELYDALDGPVAILARIDLEPAPATGGAALPAPDAAAVDSVLAAAADEDDFVWDGAQ
                     MGRVADTVLGANRLVLLVSNTERLEVGTSVNGVSGWSDVQIFEMQAGGSDWFIPFSVD
                     LATTTEPAPGEPAPSPQGYRLARRSFFAREVNADITLAKAYPALHTPTISVRSGTYER
                     WYVGNLNNSQPLDAAAGAPDMHPFHIHLVNFVVLRRWQLDATGRFVVVAPSDLDLDLI
                     ARQDTVAIPSNQLVELLVHYPIGYTGDFAYHCHILEHEDNCMMSHFHVDA"
     gene            2504..>3211
                     /locus_tag="VFF72_03515"
     CDS             2504..>3211
                     /locus_tag="VFF72_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92257.1"
                     /translation="MGWAWTAAGFALNPPDEGACEQAQGTVESTLRTWGSDVMFRSTN
                     FKSKLGARWGAGVALAVLVATLGACGGRSTPIDTTTPPDGGSGAGGVTGVAYLGPISG
                     ATASLFNVNPDGTNNGAAIEVVTTTATGFAFTHSVTAPSRICVDGGTYVDEATGTTQT
                     NTVTLCALVGGAATQVFITPMSGFVDEIVKAQLKLKTAPTLTDFTAALAAANTLIKSA
                     YSVTTTVDAIKPSFIAAD"
BASE COUNT          538 a         1035 c         1149 g          489 t
ORIGIN      
        1 acaagctcgc cgacctgcgc taccgcaacg gcgcgtcgag ctacctcgac tcgctcgacg
       61 cacagcgcgc gctcttcgtc gcgcagcagg cgctgatcca ggtccaggcc gcgcagctgc
      121 agaacaccgt ggcgctgtat cgggcgctgg ggggcgggtg gaaggacgag gcggcgacga
      181 cggcgacgcg ttgaacggcg gctcgcccgc cgtgcgctcg gggctcaggc atcgacgtgg
      241 aagtgcgaca tcatgcagtt gtcctcgtgc tcgaggatgt ggcagtgata ggcgaagtcg
      301 ccggtgtagc cgatcggata gtggacgagc aactcgacga gctggttcga aggaatggcg
      361 acggtgtctt gccgcgcgat caggtcgaga tcgaggtcgg acggtgccac gacgacgaag
      421 cggcccgtgg cgtcgagttg ccagcggcgc aggacgacaa agttgacgag gtggatgtgg
      481 aaggggtgca tgtcaggcgc acccgccgcc gcatcgagcg gctggctgtt gttcaggttg
      541 ccgacgtacc agcgctcgta ggtgcctgat ctcacgctga tggttggcgt gtgcagggcc
      601 ggataggctt tggccagggt gatatcggcg ttgacctccc gcgcaaagaa gctgcgccgg
      661 gcgaggcggt agccctgagg cgatggggcc ggctcgccgg gcgcgggctc ggtcgtggtg
      721 gcgaggtcga ccgagaaggg gatgaaccag tcggagccgc cggcctgcat ctcgaagatc
      781 tgcacgtcgc tccagccgga gacgccgttg acggaagtac cgacctcgag tcgctcggtg
      841 ttgctgacca gcaggacgag tcggttggcg cccaggacgg tgtccgcaac gcgacccatc
      901 tgcgcgccgt cccagacgaa gtcgtcttcg tcggcggctg cggcgaggac actgtcgacg
      961 gctgccgcgt ccggcgccgg caacgcagcg ccacccgtcg cgggcgccgg ctcgagatcg
     1021 atccgggcga ggatggcgac gggtccgtcg agcgcgtcgt agagctcctg atcggcttcg
     1081 tcgaagcggg cattcgtcgg cgccagcacc gagtcgtcga aggtcgtgta gatcgcctcc
     1141 agggtgagct cgtcgaccgc gagcagatag cgcagcgaga tgttgaccag gcgcagccgc
     1201 gtcaccgtcg ccggcactgc ggagagatcg acgacgacgt cgcggcgctg cgccggggcg
     1261 atgaccagcg cgtcggtgtc ctgcaacgcg accgacctgc ccatcaatcc gccatcgccg
     1321 ccgatcaggc gcatgcattg cgtgtaccag acgcggccgg tctgcgccgc gatggcgtcg
     1381 gggtcgcaaa gcgccagcgc ataggttcgc gcgttggaga gatcgagcac gcgcaaacgc
     1441 caggcgccgc gggccagcgt cagctcgggc gcgggatgtc cgttgatgaa gatctggtcg
     1501 ccgaggaatt ccggacgtcc caccgcatcg tcggagccga tgttcgacag gctgtagtcg
     1561 tggggaatgc cggcgtcgta gtcgatggcc gtctgatcgg cattcaggat ccggtctgtc
     1621 atcacgaaca ggagttcctg cgagcggccg ccgatgagcg ccaggatcgc gtcgtcggcc
     1681 gcgtcgcgta tgcaatacgg accgcagagg ccggcgtgca cgtgccgtcc gacgcggtcc
     1741 atgccgtggt cgtggtacca gagcatcgtg ccgcgctggg cattggggta gaagtcttgc
     1801 cggctctgcg ggaagccgta cggattgccg gcgaagccga ccggcgtcag cggccaaccg
     1861 tcggcgtcgg cctggacccg ggctccatgg agatgcgctg ctaggccgac cggcgactgg
     1921 ttgacgaccc ggccgcacat gcccagatcg agggggacgt tgatcggcgg atcggccagg
     1981 cgcgccggga cgaggggcga agcgccgatg gtgttggtcc acgtcaccgt gcaggcgctg
     2041 tcgcgacgga cgttgaagaa gggccccagc gagttgccca gaacgccgct ggcaggggcg
     2101 ctgccgtcgg ccacgtagac ccaggcattg gcattcgccg gagtcttgcc gacgacccac
     2161 tgcacctgcc gggcctggat cgagatcgat gccgtcgccg ggttttccag gatggtcggg
     2221 ccgggcgccg gcgccgaggg cggcagtgag gaagacggct cgggcgagga gtcgccgccg
     2281 ccgcacgccg cgagaccgat cgacgcaccc agcgggcccg cgccgatcgc ccacccgctg
     2341 ctccagcgaa aaaaggtgcg acgactgacg cgcgctgtcg catcgtcctc gagcggacgc
     2401 ccttccccat cgttgagagt catgccgatt ccccgccccc gaatcaacga aattgttggc
     2461 gcgagtgtgt gatgcgacgt tcgggctgac aagcacccct gccgtgggat gggcctggac
     2521 ggccgctggt tttgcgctga acccccccga tgaaggggct tgcgagcaag cgcagggcac
     2581 tgtggaatcc acgctccgaa catgggggag tgatgtgatg tttcgttcga caaacttcaa
     2641 aagcaaactc ggcgcaaggt ggggcgcggg cgtggcgctg gccgtccttg tcgcgacgct
     2701 cggcgcgtgt ggcggccgca gcacgcccat cgataccacc acgccgccag acggcggcag
     2761 cggcgcaggc ggcgtcaccg gcgtcgccta tctggggccg atctccggcg caaccgccag
     2821 cctgttcaat gtcaaccccg acggcaccaa caacggcgcg gcgatcgagg ttgtcacgac
     2881 cacggcgacc ggcttcgcct tcactcattc agtcaccgcg ccgtcgcgca tctgcgtcga
     2941 cggcggcact tacgtcgatg aagccaccgg cacgacgcag accaacacgg tcacgctgtg
     3001 cgcactggtg ggcggcgccg caacccaggt cttcatcacg cccatgagtg gcttcgtcga
     3061 cgagatcgtc aaggcccagc tcaagctcaa gaccgctccg acgctgaccg acttcacggc
     3121 agcgcttgcc gcggcgaaca ccctgatcaa gtcggcctac agcgtcacga cgacagtcga
     3181 cgcgatcaag ccgtcgttca ttgccgccga c
//
LOCUS       DASRWS010000193         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_938531, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000193 DASRWS010000000
VERSION     DASRWS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938531"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..721
                     /locus_tag="VFF72_03520"
     CDS             104..721
                     /locus_tag="VFF72_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZV92258.1"
                     /translation="MATSTRKPAAGKKAASKSSARKASATKKSGPARKPLRTAAKKPY
                     KDNTGDNTQPPKARPKDAVDLLVADHLAAGKCFKQYQTLVKKNGSAAARRELANKVCD
                     MLKVHTRIEEEIFYPAARAAGVDAGLLDEANVEHSSAKELIAQIEAARPGDDFYDAKV
                     KVLGDYVTHHVVEEQTEMFPKCRRSTMDLVALRRQLETMKAQLQG"
     gene            796..1518
                     /locus_tag="VFF72_03525"
     CDS             796..1518
                     /locus_tag="VFF72_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZV92259.1"
                     /translation="MDVSLVRELAPTGKLRASLNLGNPVLARSRTVAESPAGVTIDLS
                     RRLAQELGVELVFLEFATAAASVAALTSADADIGFMAIDPLRAATVHFTSAYVHIEGA
                     YLVPDDSPIERNDDVDRPGVHVVVGTGSAYELHLSRTLRHAEIVKVATSEDVVGAMLE
                     RGLEVAAGVRQQLEADARRVAGMRMLEGRFMVIRQAMAMPVTRSAAAAAFLDDFAARM
                     KAGGFVAAALARHGITGADVAP"
     gene            complement(1534..2082)
                     /locus_tag="VFF72_03530"
     CDS             complement(1534..2082)
                     /locus_tag="VFF72_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HZV92260.1"
                     /translation="MAHSYSQLVKQIESLTRKADMARKKEVAGVIGRIKEAIAFYALT
                     AEELGLRAKSLATRVHATSRHKTSHAKSKSKFGDTPKYRDEAGNEWVGRGPRPLWLRE
                     AIKSGRSLHEFAIDGAAAAKPAKAAKKATTRRKSRRGRKASTVAKYRDAATGKTWSGR
                     GRKPAWFVNALAEGKSLESMAA"
     gene            2845..>3671
                     /locus_tag="VFF72_03535"
     CDS             2845..>3671
                     /locus_tag="VFF72_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92261.1"
                     /translation="MKRTLATATAAGILLGVSVDAGAVLTLNATGIADGFSLSTFYTD
                     PGAYYGLIGAVSAPGSFAIGSGYARGQLYQFNDTDGQSFGTALTTASAGGTPTAIASV
                     GSKIYVGLLGGHYYQVDPTTLALSAITGNGINAYYGLWGNGVTGHLLAGTSQGLLDIN
                     PLDGSFFVVGQPGGNIDGVTVSPNGSIAYVETNSNALYGYSILTPNPSTPVFSRTALP
                     GGPDGTGVISGGIFDGDIVVNNNDGTVGLIDHLTGVETIIASGGSRGDLVAPDTSNGT
                     "
BASE COUNT          646 a         1166 c         1201 g          658 t
ORIGIN      
        1 ctttcacttc ttcgactgac atcatgggca agtacctttt cgcgtggatc ctcggcgttc
       61 cggcggtcct gctcgtcggc atctacgtcg tcgcgcatct gtgatggcca cctcgacaag
      121 gaagccggcc gccggcaaga aggcggcatc gaaatcgtcg gccaggaaag cctcggcgac
      181 gaagaagtcg ggcccggctc gcaagccgct aagaaccgcg gcgaagaagc cgtacaagga
      241 caacaccggc gacaacaccc agccgccgaa ggccaggccc aaggacgccg tcgacctcct
      301 ggtggccgac catctggcgg cgggcaaatg cttcaagcag taccagacgc tggtgaagaa
      361 gaacggctcg gccgcggcgc ggcgcgaatt ggcgaacaag gtgtgcgaca tgctcaaggt
      421 tcacacccgg atcgaggaag agatcttcta cccggcagcg cgcgccgccg gcgtcgacgc
      481 cggcctgctc gacgaagcga acgtcgagca ttcgagcgcc aaggagctga tcgcgcagat
      541 cgaggcggcc aggccgggcg atgatttcta cgacgccaag gtcaaggtcc tcggcgacta
      601 cgtgacccat cacgtggtcg aggagcagac cgagatgttc ccaaagtgcc gtcgctcgac
      661 gatggatctc gtcgcgcttc gtcggcagct cgaaacgatg aaggcgcagc ttcaaggctg
      721 acgcgggggc gacgccgcgc ggcgcttcgt gcaaactgac gagtggccgc ctcgtcgggc
      781 ggcggatgcg tgtcgatgga tgtgtcgctc gttcgtgaat tggctcccac cggcaagctg
      841 cgcgcgtcgc tcaatctcgg caatcccgtg ctcgcgcgca gccgcaccgt ggccgagagt
      901 ccggcaggcg tcaccatcga cctgtcgcga cgcttggcgc aagaactcgg cgtcgaactc
      961 gtctttctcg aattcgcgac agccgccgcg tcggtggccg cattgacctc cgccgacgcc
     1021 gacatcggct tcatggcgat cgacccgttg cgcgcggcga ccgtgcattt cacctccgcc
     1081 tacgtgcaca tcgaaggcgc gtacctcgtg ccggacgact cgccgatcga gcgcaacgac
     1141 gacgtcgatc ggcctggcgt ccatgtggtc gtcggcacag gcagcgccta cgagctgcat
     1201 ctcagccgaa cgctgcgcca cgccgagatc gtcaaggtcg cgacttcgga ggacgtcgtc
     1261 ggtgcgatgc tcgagcgagg cctcgaggtg gccgccggcg tcaggcagca gctcgaggcc
     1321 gatgcgcgac gggtcgcagg aatgcgaatg ctcgagggcc gcttcatggt gattcgccag
     1381 gcgatggcga tgccggtgac gcgcagcgcc gcggcggccg catttctgga cgacttcgcg
     1441 gcgaggatga aggccggcgg cttcgtcgcc gcggccctcg cccggcacgg catcacgggc
     1501 gcggacgtcg cgccctgagc cgcggggccg gcgtcaggcg gccatcgatt ccagcgactt
     1561 gccctcggcg agggcgttca cgaaccaggc gggctttcga ccgcgtcccg accacgtctt
     1621 gccagtggcg gcgtcccggt atttcgccac cgtcgatgcc ttgcggcccc gacgtgactt
     1681 gcgccgcgtt gtcgccttct tggctgcctt ggcgggcttg gccgcggcgg cgccgtcgat
     1741 ggcgaactcg tgcagcgagc ggccgctctt gatcgcctca cgcaaccaga gcggtcgcgg
     1801 gccgcgaccg acccattcgt tgccggcctc gtcgcggtat ttcggcgtgt cgccgaactt
     1861 gctcttcgac ttggcatgcg aagtcttgtg ccgcgaagtg gcgtggacgc gcgttgccaa
     1921 agacttggcc ctcagcccca attcctcggc ggtcagggcg tagaaggcga tcgcttcctt
     1981 gatccggccg atcacgccgg ccacttcctt cttgcgcgcc atgtcggcct tgcgcgtcag
     2041 ggattcgatc tgcttgacga gttgtgaata ggagtgagcc atgaattcga cgaagttgaa
     2101 gaatggttgg aattggaaga cgcgttggcg attctatgtc gggacgatcc gacttgaata
     2161 aggtgtcgat tcgacgcggg atttgaaaga ggattggtct tcgccgtgac gagcgggcat
     2221 gaggactgca attccgggcg aggggccctc gcagctcggg cccatggtcg ccggcagcaa
     2281 agaacgtctt tggtcgcacc gagcgtaatc ggcttcgaac cctcgacgtt tgaacatacg
     2341 gtgaacgact catgccatcg aatcgtctcc cgctcgctcg aacgtcgtgg agaaatcgcc
     2401 ctggatgcgt tcctgtttgc acgtcggctg ctcaaagcgg acattggccg cttccattgg
     2461 tcaaaggcag gggcatttcg gcagccgacg tttgacgtgg cagacgtcga gtgttcgcct
     2521 gtctgggcgg caaggtggcg ttgtcaaccc gctttcgact ttcgcgtggc tgcactgggc
     2581 gcttgaacga cgtcgttcat cacgacaagt caagacactg gccgcgagcc cggtcagcgg
     2641 tcgtcgttgg ctcaaagcga gccgcgaacg agaccgccac tgtgcccacc tccccccggg
     2701 aatgaggggg ttgcggcggc cggcgcgcgg gaggaaatta gcggcgcgct gagacaaacc
     2761 tcggtgtctc ggcgctaccg ctcacggacg accagcttat cggaggtcca tatgaatgtt
     2821 gagagtcgat atttcttgcg tggagtgaag cgaaccctcg caacggccac ggccgccggc
     2881 attcttctcg gcgtcagcgt cgacgccgga gcggtgctga cgctgaatgc gaccggcatc
     2941 gccgacggat tctccttgtc gaccttttat accgatcctg gcgcctatta cgggctaatc
     3001 ggcgcggtca gtgccccggg gagcttcgcg atcggctccg gatatgcgcg aggccagctg
     3061 taccagttca acgacacgga cggccaatca ttcggaactg cactgacgac ggcgtcggct
     3121 ggcggtacgc cgacggcgat cgcttcggtg ggcagcaaga tctatgtcgg cctcctcggg
     3181 ggccactact accaggtcga tccgacgacc ttggctctgt cggcgattac tggcaacggg
     3241 atcaacgcgt actacggcct gtggggcaac ggcgtgacgg gacacctgct cgccggcacg
     3301 tcacagggtc ttctcgacat caacccgctc gacggcagct tcttcgtcgt gggtcagcca
     3361 ggcggcaaca tcgacggcgt caccgtttcg ccgaacggct cgatcgccta cgtcgagacg
     3421 aacagcaacg cgctgtacgg ctacagcatc ctgacgccga acccaagcac gcccgtcttt
     3481 tcacggaccg cactacctgg cggcccggat ggcactggag taatcagcgg cggcatcttc
     3541 gacggagaca tcgttgtcaa caacaacgat ggcaccgtcg gattgatcga tcatcttact
     3601 ggcgtcgaaa cgatcatcgc gtcgggtggc agtcgcggcg acctggttgc gcccgatacg
     3661 agcaatggca c
//
LOCUS       DASRWS010000194         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3817425, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000194 DASRWS010000000
VERSION     DASRWS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3817425"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFF72_03540"
     CDS             <1..488
                     /locus_tag="VFF72_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HZV92262.1"
                     /translation="DGKSELPVFTTVLERKLVLDSGPWSQALGDDEKGYVMLLRRLEI
                     GGRFDCSSRMFLPASRFGRLLRIAARRLADTNLKMLLESDFAAPTLHSDGVAHVQRCA
                     QADARLIGVSAGSPALQVQICTYSFGRTPITWQRMYVPATGHGLKLDFNPPRAAANAR
                     P"
     gene            509..>2523
                     /locus_tag="VFF72_03545"
     CDS             509..>2523
                     /locus_tag="VFF72_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HZV92263.1"
                     /translation="MTRNAHIARLSYPPETLGHVRVEADPQTVAGSMATVRIVYTAGK
                     FGIDDQGSLRFLLRFASDAGRPQFDRPAAPNYCTATASNGTTLLVEYHPRGAFRPWFK
                     AIRVNVMREALHEGDTITLVLGDRSQGGAGWRTSTMREERFEVRVQVDPFGTVVYGDV
                     AGHATLDLVPGDAHVWKAVLPTLRRPGEPFELRIRADDACGNPVRRLPGSVSLSAVGA
                     IEGLPARVAADGATIVVGGLRASAPGVVRIAVRDVAGHLLVESNPLVVADAAGPSTWW
                     GDFHAQSEETIGTNSARSYFEFARDLAFCDFVGHQGNDFQITGEFWAELNRLYREFDA
                     PGRFVTLPGYEYSPVTHLGGDRNIFFFDEGRPIRRSSHALVEDLSDIDSDCNHVLDLF
                     AALKRNGENALAFAHVGGRYADLHAGHDVAIERSVEVHSAWGTFEWLLFDAFELGHRV
                     GVVCNSDDHKGRPGASHPGASIFGAYGGLTCLYLDALTRADIWSCMQSRHHYGTTGDR
                     LHLDVRATFAQEARRFAVDPKLDPAARHDPVREAMMGDIVSVASANATIRVRIDAVSP
                     IERVDLRAGARTLETVRPFSERDCGRRIRVIWEGAEYRGRGRMTTWDGSLRVGGNTIE
                     SFSPINFWHLDKSLARREPDMLAWESVTTGNFAGADIVFAETSGGRI"
BASE COUNT          375 a          932 c          825 g          391 t
ORIGIN      
        1 tcgacgggaa gagcgagctg ccggtcttca ccacggtgct cgaacgcaag ctcgtcctcg
       61 acagcggacc gtggtcgcag gcgctcggcg acgacgagaa gggctacgtc atgctgctgc
      121 gccggctcga gatcggcggc cgcttcgatt gcagcagccg catgttcctg ccggcgagcc
      181 gcttcggccg actcctccgg atcgcggcgc gccggctggc cgacaccaac ctgaagatgc
      241 tgctggagtc ggacttcgcg gcgccgacgc tgcactccga cggcgtcgcc cacgtccagc
      301 gctgtgcgca ggccgacgcg aggctgatcg gtgtcagcgc cggatcgccc gcactgcagg
      361 tgcagatctg cacctattcg ttcggccgca cgcccatcac ctggcagcgg atgtacgtgc
      421 cggcaaccgg tcacggcctc aagctcgatt tcaacccgcc gcgggccgcg gcgaatgctc
      481 gcccctgatt cgcctgtttc gccccgccat gacccgcaac gcccacatcg cccgcctgtc
      541 ctatccgccg gagacgctcg gccacgtgcg ggtcgaggcc gatccgcaga ccgtcgccgg
      601 ctcgatggcc acggtgcgca tcgtctacac cgccggcaag ttcggcatcg acgatcaggg
      661 cagcctgcgc ttcctgctgc gcttcgccag cgacgccggc cggccgcaat tcgaccgccc
      721 ggccgcaccc aactattgca ccgcaacagc gtcgaacggc accacgctgc tcgtcgaata
      781 tcaccctcgc ggcgcgttcc ggccctggtt caaggcgatc cgcgtcaacg tcatgcgcga
      841 agcgctgcac gagggcgaca cgatcacgct cgtcctcggc gaccgatcgc agggcggcgc
      901 gggctggcgc acgtcgacga tgcgcgagga gcgattcgag gtgcgcgtgc aggtcgaccc
      961 gttcggcacg gtcgtctatg gcgacgtcgc cggccacgcc acgctcgacc tcgtgccggg
     1021 cgatgcccac gtctggaagg ccgtgctgcc gacgctgcgc cgacccggcg agccgttcga
     1081 attgcgaatc cgcgctgacg acgcctgcgg aaacccggtt cgccgcttgc ccggcagcgt
     1141 ctcgctgagt gcggtcgggg cgatcgaagg cctgccggcg cgcgtcgccg ccgacggcgc
     1201 gacgatcgtc gttggcggcc ttcgcgcatc ggcacctggc gtcgtgcgca tcgcggtgcg
     1261 cgacgtcgcg ggccacctgc tcgtcgagtc caatccgctc gtcgtcgccg acgccgccgg
     1321 accctcgacg tggtggggcg acttccatgc gcaaagcgag gagacgatcg gcacgaacag
     1381 cgcgcgcagc tacttcgagt tcgcccgcga cctggcgttt tgcgacttcg tcggccacca
     1441 gggcaacgac ttccagatca ccggcgagtt ctgggccgag ctcaaccgcc tctatcgcga
     1501 gttcgacgcg cccggccgct tcgtgacgct gccgggctac gaatactcgc ccgtcaccca
     1561 ccttggcggc gaccgcaaca tcttcttctt cgacgagggc cggccgatcc gccgctcgtc
     1621 gcacgcgctg gtcgaagacc tgtcggacat cgacagcgac tgcaatcacg tcctcgacct
     1681 cttcgcggca ctgaagagga acggcgagaa cgcgctcgcc ttcgcccacg tcggcggccg
     1741 ctacgccgac ctgcacgcgg gacacgacgt cgccatcgag cgctcggtcg aggtccactc
     1801 ggcctggggc accttcgagt ggctgctctt cgacgcgttc gagctcggcc accgcgtcgg
     1861 cgtcgtctgc aattccgacg accacaaggg acgccccggc gccagtcatc cgggcgcctc
     1921 gatcttcggc gcctatggag gcctgacctg cctctatctc gacgcgctga cgcgcgccga
     1981 catctggtcg tgcatgcagt cgcggcatca ctacggcacc accggcgacc gcctccatct
     2041 cgacgtgcgc gccaccttcg cgcaggaggc taggcgcttc gcggtcgatc ccaagctcga
     2101 ccccgcggcc cgccacgatc cggtacgcga agcgatgatg ggcgacatcg tctcggtcgc
     2161 gtccgccaac gcgacgatcc gggttcgcat cgacgccgtg tcgccgatcg agcgcgtcga
     2221 cctgcgcgcc ggggcacgga cgctggagac ggtccgtccc ttttccgaac gcgactgcgg
     2281 ccggcgcatc cgagtgatct gggaaggggc cgagtaccgc gggcgcggcc gcatgacgac
     2341 ctgggacgga tcgctgcgcg tcggcggcaa caccatcgag agcttctcgc cgatcaactt
     2401 ctggcacctc gacaagtcgc tggcccggcg cgagcccgac atgctggcct gggagtcggt
     2461 gacgaccggc aacttcgcgg gggccgacat cgttttcgcc gaaacgtcgg gtggccggat
     2521 cga
//
LOCUS       DASRWS010000195         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1230872, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000195 DASRWS010000000
VERSION     DASRWS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230872"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VFF72_03550"
     CDS             <1..1069
                     /locus_tag="VFF72_03550"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZV92264.1"
                     /translation="VHRAVDEGLSFGAPTGREVDLAEAILRHVPSMEQVRLVSSGTEA
                     AMSALRLARGATGRDVIVKFEGCYHGHADALLVKAGSGLATFGHPTSAGVPQAVVQDT
                     LVLEYNNLDELAAAFARHGKRIACAIVEPIAGNMNFVRASVPFMRSLRELCSSHGALL
                     ILDEVMTGFRVGLGGAQALYARSLPGFAPDLSVFGKVIGGGMPLAAFGGKRAVMEQLA
                     PLGPVYQAGTLSGNPVATACGLATLHEIEKPGFFEALARTTRRLVAGLIEAAGAAGVP
                     MTGDCEGGMFGFFFAGELPQRYADVMGSDGARFNRFFHAMLERGVYLAPALYEAGFVS
                     AAHTPADVDATVAAAAASLAA"
     gene            1088..2470
                     /gene="mpl"
                     /locus_tag="VFF72_03555"
     CDS             1088..2470
                     /gene="mpl"
                     /locus_tag="VFF72_03555"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZV92265.1"
                     /translation="MHLHVLGICGTFMGGLAALAREAGHRVTGCDANVYPPMSGQLAA
                     LGIELIEGYDAGQLKLRPDVFVVGNVVTRGNALMEAVLDAGQPYTSGPQWLAGHVLAG
                     RHVLAVAGTHGKTGTTSMLARILDAAGRAPGFLVGGVPLDFGVSARLGAADAPFVIEA
                     DEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFDDLAAIERQFHHLVRTVPHSGRL
                     VVNARDEALQRVLAMGCWSEVQRFGGRKEEPGMLRARGEPHAFDVLRGGLKVARVDWS
                     LLGEHNQLNALAAIGAAEHVGVAAEVAAAALARFRNVRRRLELRGEAAGVAVYDDFAH
                     HPTAMRTTLDGLRRRVGQARILVAFEPRSNTMKLGAMKAQLPWALEEADLAFCHSGGL
                     GWDASAALAPLGQRAAVEATVPDLVARIVAAARPGDHVVCMSNGAFGGIHEKLLAALA
                     AAGPARTAAG"
     gene            2467..3084
                     /locus_tag="VFF72_03560"
     CDS             2467..3084
                     /locus_tag="VFF72_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HZV92266.1"
                     /translation="MNDAPVTHLLYLHGFRSSPKSTKARMMAAWMREHRPGVIWACPQ
                     LPPSPCEAMACIDAAVAAWPLAGMAVIGSSLGGFYATVLAERNGCRAVVLNPAVDPAR
                     DLADRVGEQKSWHDDDTFFFRPGYIDELRAMAPPPSLTRPDRYFAVIAKGDELLSWRE
                     MKSRYAGSDGVLIEGSDHALSDFADHLPAVVRFLQLGAARSAGSS"
     gene            complement(3088..3300)
                     /locus_tag="VFF72_03565"
     CDS             complement(3088..3300)
                     /locus_tag="VFF72_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92267.1"
                     /translation="MIVFRWIVGVLAAFFAVAALLGFAFHVAFDSSVWLGRARRMGRG
                     LWLIMLLWFNVEVWGRVAYTLIHWRH"
     gene            complement(3329..>3584)
                     /locus_tag="VFF72_03570"
     CDS             complement(3329..>3584)
                     /locus_tag="VFF72_03570"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZV92268.1"
                     /translation="YKVLNSDGLRYGAEFVKHKILDAIGDMMTAAHPLLASYRAFKSG
                     HALNNRLVRALLSDPSSHEVVTFDDEASAPAGLAELAPAW"
BASE COUNT          521 a         1284 c         1254 g          525 t
ORIGIN      
        1 cgtgcaccgc gccgtcgacg aaggcctgtc gttcggcgcg ccgaccgggc gcgaggtcga
       61 cctcgccgag gcaatccttc gccacgtgcc gagcatggag caggtgcgcc tcgtcagctc
      121 cggcaccgag gcggcgatga gcgcgttgcg gctggcgcgg ggagcgaccg gccgcgatgt
      181 catcgtcaag ttcgagggtt gctaccacgg ccacgccgac gcgttgctcg tcaaggccgg
      241 atcgggactc gccaccttcg gccatccgac cagcgccggc gtgccgcagg ccgtcgtcca
      301 ggacacgctg gtcctcgagt acaacaatct cgacgagctc gccgcggcgt tcgcccggca
      361 cggcaagcgc atcgcctgcg cgatcgtcga gccgatcgcc ggcaacatga acttcgtccg
      421 cgccagcgta cccttcatgc gcagcctgcg cgagctgtgc agcagccacg gcgccctgct
      481 catcctcgac gaggtgatga ccggctttcg cgtcgggctc ggcggcgcgc aggcactcta
      541 cgcccgctcg ctcccggggt tcgcgcccga cctcagcgtc ttcggcaagg tgatcggcgg
      601 cggcatgccg ctggccgcat tcggcggcaa gcgcgcggtg atggagcagc tcgcgccgct
      661 cggcccggtc taccaggcgg gaaccttgtc gggcaacccc gtggcgaccg cctgtggcct
      721 ggcgaccttg cacgagatcg agaagcccgg cttcttcgaa gcgctcgcac gaacgacgcg
      781 tcggctggtt gccggcctga tcgaggccgc cggcgccgcc ggcgtgccga tgaccggcga
      841 ttgcgagggc ggcatgttcg gcttcttctt cgccggcgag ctcccgcagc gctacgccga
      901 cgtgatgggc agcgacggcg cgcgcttcaa tcgcttcttt cacgcgatgc tcgagcgtgg
      961 cgtctacctc gcgccggccc tctacgaagc cggcttcgtc agcgccgcgc acacgcccgc
     1021 cgacgtcgac gccaccgtcg ccgccgccgc cgcgagcctc gccgcctgac caggcgcgcg
     1081 aaccggcatg cacctacacg tcctcggcat ctgcggcacg ttcatgggcg gcctcgcggc
     1141 gctggcacgc gaggcgggcc atcgcgtgac cggctgcgac gccaatgtct atccgccgat
     1201 gagcgggcag ctggcggcgc tcggcatcga gctcatcgaa ggctacgacg ccgggcagct
     1261 gaagctgcgg ccggacgtgt tcgtcgtcgg caacgtcgtc acgcgcggca acgcgctgat
     1321 ggaggcggtg ctcgacgccg gacagcccta cacgagcggc ccgcagtggc tggccgggca
     1381 cgtgctggcc ggccggcatg tcctcgccgt cgccggcacg catggcaaga ccggaacgac
     1441 atcgatgctc gcgagaatcc tcgacgcggc cggacgcgcg cccggattcc tggtcggcgg
     1501 cgtgccgctc gatttcggcg tctcggcgcg actcggggct gcggacgcgc ccttcgtcat
     1561 cgaggccgac gaatacgaca ccgccttctt cgacaagcgc agcaagttcg tccactaccg
     1621 gccgcgcacc gcgatcctca acaacctcga gttcgaccac gccgacatct tcgacgacct
     1681 cgccgcgatc gaacggcagt tccaccacct ggtgcgcacc gtgccgcaca gcggccggct
     1741 cgtcgtcaat gcgcgggacg aggcgttgca acgcgtcctg gcgatgggtt gctggagcga
     1801 ggtgcagcgc ttcggcggcc gcaaggaaga gccgggcatg ctgcgggcgc gcggcgagcc
     1861 gcatgccttc gacgtgctgc gcggcggcct gaaggtcgcc cgcgtcgact ggtcgctgct
     1921 cggcgagcac aaccagctca acgcgctggc agcgatcggc gcggccgagc atgtcggcgt
     1981 cgcggccgag gtcgccgccg cggcgctggc acgctttcgc aacgtccgcc gacggctcga
     2041 gctgcgcggc gaggcggcgg gcgtcgccgt ctacgacgac ttcgcgcatc acccgacggc
     2101 gatgcgaacg acactcgacg gcctgcgccg gcgcgtcggc caggcccgca tcctggtcgc
     2161 cttcgagccg cgttcgaaca ccatgaagct gggcgccatg aaggcgcagt tgccctgggc
     2221 gctggaagaa gccgacctcg cgttctgtca ttcgggaggg ctcggctggg acgcttcggc
     2281 ggcactcgca ccgctcggcc agcgggcggc ggtcgaggcg acggtgcccg atctcgtcgc
     2341 ccgcatcgtc gccgcggcgc ggccgggcga tcacgtcgtg tgcatgagca acggcgcctt
     2401 cggcggcatc cacgagaagc tgctcgccgc gctcgccgcc gccggaccgg cgcggacggc
     2461 ggcgggatga acgacgcgcc tgtcacccat ctgctctacc tgcacggctt tcgctcgtcg
     2521 ccgaaatcga ccaaggcgcg aatgatggcg gcctggatgc gcgagcatcg gcccggcgtg
     2581 atctgggcgt gcccgcagct gccgccatcg ccgtgcgagg cgatggcgtg catcgatgcg
     2641 gccgtcgctg cctggccgct cgccggcatg gcggtgatcg gcagctcgct gggcgggttc
     2701 tacgccaccg tgctggccga gcggaacggg tgtcgcgccg tcgttctcaa ccccgcggtc
     2761 gatccggcgc gcgacctggc cgaccgcgtc ggcgagcaga aatcctggca cgacgacgac
     2821 acgttcttct ttcgtcccgg ctacatcgac gagctgcggg cgatggcgcc gccgccctcc
     2881 ctcacccgac cggatcgcta cttcgccgtc atcgccaagg gcgacgagct gctgtcgtgg
     2941 cgcgagatga agagtcgcta tgccggcagc gacggcgtgc tcatcgaagg cagcgaccac
     3001 gccctgagcg acttcgccga tcacctgccc gctgtcgttc gcttcctgca actcggcgcg
     3061 gcccgctccg ccgggtcttc gtgagggtca gtgtcgccag tggatcaggg tgtaggccac
     3121 ccggccccag acctcgacat tgaaccacag cagcatgatc agccacaggc cgcggcccat
     3181 gcgccgggcg cggccgagcc agaccgacga gtcgaaggcg acatggaacg cgaaaccgag
     3241 gagggcggcg acggcgaaga acgccgcgag cacgccgacg atccagcgaa agacgatcaa
     3301 tgtcgtcggc tccgacgcgg gtcgcgattc accatgcggg cgccagctcg gcgaggccgg
     3361 cgggagcgct cgcctcgtcg tcgaaggtga cgacttcgtg gctggacggg tcggaaagaa
     3421 gcgcccggac gaggcggttg ttgagcgcgt gccccgactt gaaggcgcgg tacgacgcca
     3481 gcagcgggtg cgccgccgtc atcatgtcgc cgatcgcgtc caggatcttg tgcttgacga
     3541 actcggcgcc atagcgaagg ccgtcgctgt tgaggacctt gtat
//
LOCUS       DASRWS010000196         4894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2660056, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000196 DASRWS010000000
VERSION     DASRWS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660056"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFF72_03575"
     CDS             complement(<1..316)
                     /locus_tag="VFF72_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZV92269.1"
                     /translation="MSDGAAESGAPAFANRLHSFRPRLLDALADYDRRRFAADLAAGV
                     TVGIVALPLAMAFAIASGVKPEQGLFTAIIAGFVISALGGSSVQIGGPAGAFIVIVYG
                     IVA"
     gene            558..989
                     /locus_tag="VFF72_03580"
     CDS             558..989
                     /locus_tag="VFF72_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HZV92270.1"
                     /translation="MCPRLRRLRPLKMADYLVVKAVHESAVALSGLGFLARGAGAFSG
                     ADWVRHRVARSLPHVVDTVLLLSAITLVWMLRIDPRSAPWLMAKIVALPVYIGLGVVA
                     LRPAMPRRARVTAWLAALACFAYIVSVAITKSPLGVLAAFR"
     gene            complement(1125..1949)
                     /locus_tag="VFF72_03585"
     CDS             complement(1125..1949)
                     /locus_tag="VFF72_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZV92271.1"
                     /translation="MPELKSILLHVDATAASITRLVVSQQLAALHAARVTALYAAAAE
                     TGRVAFACSTAASLRSQEEWEPANVESRRRLQRLADTRGPRVEWSDVIGEEVDRAFLR
                     EAAYADLVVVGSPASEAPAGSVESGFAETMILESGRPVLVLPIEPRSSIVGRRVLVAW
                     DGSAPAARALTASLPILQHAEQVHVASWSDRPLAAPFSRVDVGRYLEEHDVVATRHGR
                     MATTRVADEVLAMATSLDADLVVMGCYGHSRTRERLFGGTSRRVLARMPVALLMAH"
     gene            2279..3094
                     /locus_tag="VFF72_03590"
     CDS             2279..3094
                     /locus_tag="VFF72_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZV92272.1"
                     /translation="MRTEPSPSIQQSGIDDTIAAATEPLPHGAGAGAADAAVDAGRHA
                     NERIGDAMRLLDERLAPQRRVVRADDVIYRAGESFTHLYVLNSGCVKIVSLADDGREQ
                     LVGLKLRGDWLGFDGIADGRYACDAIAVDTGDIRVLRYDRLIEAGVSCTPLLAALHEA
                     MSRELGRDRESLMSLCTLPADARVAEFLRRWADSLARRGLRCDEISLRMTRAEIGNHL
                     GMTLESVSRALSRLARDQIIAFAGKGRRDIDIPHVAALSAFVQRCIASPRVLQ"
     gene            complement(3112..>4894)
                     /locus_tag="VFF72_03595"
     CDS             complement(3112..>4894)
                     /locus_tag="VFF72_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZV92273.1"
                     /translation="RRKDGSEVGVEIALSPLQGAHGPLVVAAIRDIGAYPRMKQALQR
                     AHYSEQLAVLGRLAVDARDPQELLDRVPEVAAEALGVDVARVYLLETNLQQLRVASAV
                     GSVPGEPPGELLANRPDTSLGFVLAQGRSVIVSDYATEQRFSVPQGYLDVGLTSALAV
                     PISDRGRTVGVLAARSRGQRLFADDDVRFLESLANLLAGSLQRVRSEEALSHAERLES
                     VGQLTGGIAHDFNNLLTVIQGNLQVLDELPLLAGDGYARQLVAAAARASRRGAELTGK
                     LLAFSRRQVLQPVGVDVGALLQSLADMLRRTFDQRIRIDVDVDAATPLALADPGQLES
                     ALLNIAINARDAMPAGGALRFGAVHCADAPQDIRGEIEDADALSAGLVAIAVSDDGTG
                     MTEEARERAFEPFFTTKGAGRGTGLGLSTVYGFVKQSKGAITIDSALGRGTQVTLYIP
                     ALREVADAPLPRRERHLVVPPGLRVLLVEDDAQVRTIMHRFLDAMRCRVTAAASGEQA
                     LRAVAQGDGFDLLVTDIALGAGIRGTELATRIQRQCPGLAVLLVSGYSAELIDADRDA
                     PATWQLLRKPCSREELGHAVALAIAAA"
BASE COUNT          727 a         1744 c         1716 g          707 t
ORIGIN      
        1 gagcgacgat gccgtagacg atgacgatga atgcccccgc cgggccgccg atctgcacac
       61 tcgatccacc gagggcggag atgacgaagc cggcgatgat cgccgtgaaa agcccctgct
      121 cgggcttgac gccgctggcg atcgcaaagg ccatggccag cggcagggcg acgatgccga
      181 cagtgactcc ggccgcaaga tcggccgcga agcggcggcg gtcgtagtcg gcgagcgcgt
      241 ccagcagccg cggccggaac gaatggagcc ggttcgcgaa cgccggcgcg cccgattcgg
      301 cggcgccgtc gctcacgccg ccgacgccag gagaaacgca ccggtgccgt tctcgcccgc
      361 caacggcgcc ggtgcggcgc cgaagcggtc gatcgtgaag gcggtcatcg gcacatggag
      421 aagttcccgg cgccgatcgt aggcagcgag ccggggtcgc gattgcggga tgtcaagcga
      481 cggacagccg ctggccgcat agtcgcgatc gaagcgatcg cctcgtcctg cgccgtgatt
      541 ccgattcgtc cccgctcatg tgtccacgcc ttcggcgctt gcggccgctg aagatggcgg
      601 actatctcgt cgtcaaggcc gtccacgaaa gcgcggtcgc gctctcgggg ctcggctttc
      661 tcgcccgcgg cgccggcgcg ttttcgggag cggattgggt gcgccatcgc gtcgctcgtt
      721 cgttgccgca tgtcgtcgac accgtgttgc tgctatcggc gatcacgctc gtctggatgc
      781 tgagaatcga tccgcgctcc gctccctggc tgatggccaa gatcgtcgcc ctgcccgtgt
      841 acatcggcct cggcgtcgtc gcattgcgtc cggcgatgcc acgccgagcg agggtgaccg
      901 cctggctcgc ggcgctcgcc tgcttcgcct acatcgtgtc ggttgccatc acgaagagcc
      961 cgctcggcgt gctcgccgcg ttcagatgag catcgggccg cgaggcccgg cgccgcccat
     1021 acatcgcatc accgctgacc ggcgacgacg tcgagggtcc tgcgagcggt tcccgtcggg
     1081 tcgtcggcaa cgccgccgcc gtcgacatcc ggccatcgac cggctcagtg cgccatcagc
     1141 agcgcaaccg gcatccgcgc cagaacgcgg cgcgaagtgc cgccgaacag ccgctcccgc
     1201 gtccggctgt gtccgtagca gcccatgacg accagatcgg catcgaggct cgtcgccatg
     1261 gcgagcactt cgtccgcgac ccgggtcgtc gccatgcgcc cgtggcgggt cgcgaccacg
     1321 tcgtgttcct cgaggtagcg gccgacgtcg acacggctga acggcgccgc caacggccgg
     1381 tccgaccacg aggccacgtg gacctgctcg gcatgttgca ggatcggcag cgacgcggtg
     1441 agggcgcggg ccgcgggtgc cgatccatcc caggcgacca ggacccgccg gccgacgatg
     1501 ctcgatcgcg gctcgatcgg caggacgagg acaggacggc cgctttccag gatcatcgtt
     1561 tccgcaaaac cgctctcgac gctgcccgcc ggcgcctcgc tcgccggcga cccgacgacg
     1621 accagatcgg cgtaggccgc ctcccgaagg aaggcccgat cgacctcctc gccgatcacg
     1681 tcggaccact ccacccgcgg accgcgggta tcggccaggc gctgcaagcg gcgtcgcgac
     1741 tcgacgttgg ccggttccca ctcttcctgg ctcctcagcg aggcggctgt cgagcaggcg
     1801 aaggcgacgc ggccggtctc ggcggctgcg gcatagagcg ccgtgacgcg tgccgcgtgc
     1861 agcgcggcaa gctgttgcga gacgacgagg cgcgtgatgc tggccgccgt ggcgtcgacg
     1921 tggaggagga tcgacttgag ttccggcatg cgtcgctcgc tagacctcgc cgacgccccg
     1981 cgcgaccgcg ggtgccggcc cacgagcgcg tcggcccggt tcgcagcgca tgccgattcg
     2041 atgcatccgg caaagactag aggaggcgcc ggatcggacc ttgacgcgca tcaaggacct
     2101 ggccgccacg ccgggcgaat gcgcttcggg atcacccagg aattcgtcac gcgtgccggg
     2161 tgcgaaggtg ccgaagggcc cgcgtcgtca ccgagcggac acgcgccgtt cacacaccgc
     2221 taccgaaaga cccgcgccgt tgacacgccg ttgccgcccg attcctacca tgcgtcccat
     2281 gcgcaccgaa ccctctccat cgattcagca gtccggcatc gacgacacga tcgcagcagc
     2341 aaccgagccc ctgccgcatg gcgctggtgc cggcgccgcc gatgccgccg tcgacgccgg
     2401 gcgccacgcc aacgagcgca tcggcgacgc gatgcggctg ctcgacgagc ggctcgcgcc
     2461 gcagcggcgg gtcgtgcggg ccgacgacgt catctatcgg gccggcgagt ccttcactca
     2521 cctgtacgtc ctcaattccg gttgcgtgaa gatcgtcagc ctcgccgacg acggtcgcga
     2581 acaactggtc ggcctgaaat tgcgcggcga ctggctgggc ttcgacggca tcgccgacgg
     2641 ccgctacgcc tgcgacgcca tcgccgtcga caccggcgat atccgcgtcc ttcgctacga
     2701 caggttgatc gaggccggcg tctcctgcac gccgctgctg gccgccctgc acgaggccat
     2761 gagtcgcgag ctcgggcgcg atcgcgaatc gctgatgtcg ctctgcacgc tgcccgccga
     2821 cgcacgcgtc gccgaattcc tgcgccgctg ggccgattcg ctggctcgca ggggcttgcg
     2881 ctgcgacgag atctcgctgc ggatgacgcg cgccgagatc ggcaaccatc tcggcatgac
     2941 gctggagagt gtcagccggg cgctctcccg gctcgcccgc gaccagatca tcgccttcgc
     3001 cggcaaaggc cgccgagata tcgacattcc gcacgtcgcg gcgctgtcgg ccttcgtcca
     3061 gcgctgcatc gcctcgccgc gcgtcctgca atagccggcg gagacgctcg ctcaggcggc
     3121 ggcgatggcc agcgccacgg cgtgccccag ctcctcgcgg ctgcagggct tgcgcagcaa
     3181 ctgccatgtc gccggcgcgt cgcggtcggc atcgatcagc tcggccgagt agcccgacac
     3241 cagtagcacc gccaggcccg ggcattggcg ctggatgcgc gtcgccagct cggtgccgcg
     3301 gatgccggcg cccagggcga tgtcggtgac cagcaggtcg aagccgtcgc cttgcgccac
     3361 cgcacgcaac gcctgctcgc cgctggccgc cgcggtgaca cggcagcgca tcgcgtcgag
     3421 gaagcggtgc atgatggtgc ggacctgcgc atcgtcctcg accagcagga cacgcaagcc
     3481 cggcggcacg acgagatgcc gctctcgccg tggcagcggc gcgtcggcca cctcgcgcag
     3541 cgccgggatg tacagcgtga cctgggtgcc gcggccgagg gcgctgtcga tcgtgatcgc
     3601 tcctttcgac tgcttcacga agccgtagac tgtgctgagg ccgaggccgg tgccgcgacc
     3661 ggcgcccttg gtcgtgaaga agggctcgaa ggcgcgctcc cttgcctcct ccgtcatgcc
     3721 ggtgccgtca tcggagacgg cgatcgcgac caggcccgcg gacagcgcat ccgcgtcctc
     3781 gatttcgccg cggatgtctt gcggcgcgtc ggcgcagtgg acggcgccga agcgcagggc
     3841 gccgccggcc ggcatcgcgt cgcgggcatt gatcgcgatg ttgagcagcg ccgactcgag
     3901 ctgcccgggg tcggccagcg cgagcggggt cgcggcatcg acatcgacgt cgatgcggat
     3961 gcgctggtcg aacgtgcggc gaagcatgtc ggccagcgat tgcagcagcg cgccgacgtc
     4021 cacgcccacc ggctgcagca cctgacggcg cgaaaacgcg agcagcttgc cggtgagctc
     4081 ggcgccgcga cgcgaggccc gggcggcggc ggcgacgagc tggcgcgcgt agccgtcgcc
     4141 ggccagcagc gggagctcgt cgagaacctg caggttgccc tggatgacgg tcagcaggtt
     4201 gttgaagtcg tgggcgattc ctccggtcag ctgaccgacg ctttcgagac gttcggcatg
     4261 gctgagcgcc tcctcggagc ggacgcgctg caagctgccg gccagcaggt tggccagcga
     4321 ttccaggaag cggacgtcgt cgtcggcgaa cagccgctgg ccgcgcgaac gtgccgccag
     4381 gaccccgacg gtccggccgc gatccgagat gggcacggcg agtgcgctcg tcaatccgac
     4441 gtcgaggtaa ccctgcggga cggaaaagcg ttgctcggtc gcgtagtccg agacgatgac
     4501 cgagcgcccc tgcgcgagga cgaagccgag cgaggtgtcg ggccggttgg cgagaagctc
     4561 gccgggcggc tcgcccggca cgctgccgac cgcgctcgcg acccgcagct gctggaggtt
     4621 ggtctcgaga aggtagaccc tcgcgacgtc gactcccagg gcctcggccg cgacctcggg
     4681 gaccctgtcc agaagctcct gcggatccct ggcgtcgacg gcgaggcggc cgaggaccgc
     4741 gagctgctcg ctgtagtgcg cgcgctgcag cgcctgcttc atccgcggat aggcgccgat
     4801 gtcgcggatc gccgcgacga cgagcggccc gtgcgcgccc tgcaaggggc tgagcgcgat
     4861 ctcgacacca acttccgagc cgtccttgcg gcga
//
LOCUS       DASRWS010000197         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_654828, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000197 DASRWS010000000
VERSION     DASRWS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654828"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="VFF72_03600"
     CDS             <1..2197
                     /locus_tag="VFF72_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HZV92274.1"
                     /translation="DASLIVQANYEEHLEQLRECDLIIEAIAERMDWKLDLYKKIAPF
                     VAPHAIVASNTSGLSITRLSEALPDEIKPRFCGIHFFNPPRYMALVELIATPATDPHV
                     LDDLEAFVTTTLGKSVVRAKDTPNFVANRVGIAGMLATMKEAETYGLTYDVVDDLTGK
                     KLGRASSGTFRTADVVGLDTMAHVIKTLQDNLKDDPFYASYATPPVLDKLIAAGALGQ
                     KSGAGFYKKVGKDILRYEPKTGEYVPGGGKADEIVARILKKPPAERLKLLHDSKNPQA
                     QFLWAILRDSFHYAAVHLADIAESARDVDFAMRWGFGASQGPFELWQQAGWTQVAKWV
                     QEDIDAGKALSKAPLPSWVFEGLGTADGGVHRPEGSYSAAHARFDASSTLPVYQRQAF
                     RESVFGSGAAEPLKSGTEVWKNAEVRVWTLDGQVLIASITAKLHLISPAVTEGLLKAV
                     ELAEASYQGLVIWSPDDVFSAGANLEALMPVFMQGGGKAIAPEEKKLQDAMLRIRYAN
                     VPVVAAVRGMALGGGCELAIHCARRVAAMESYMGFVEVGVGLIPGGGGLAYVARRAAE
                     MATAGGANAYIFDFLRDAFTSAATARVGTSAIESRKLGYLLWSDVIVAHKDELLHVAT
                     AQAKAMADSGWRPPARTPFPVVGRSGIATIKGQLTNMKDGGFISAHDFHVAALIADVV
                     CGGDVDAGSLVTEEYLMALERKHFCGLLDHPKTQERIMGMLQTGKPVRN"
     gene            2304..3518
                     /locus_tag="VFF72_03605"
     CDS             2304..3518
                     /locus_tag="VFF72_03605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZV92275.1"
                     /translation="MSTKQVQEAYIVAATRTPIGRSGRGFFRNTRPDDLLIAAIHGAL
                     KQVPSLDPKAIEDAIIGCAMPEGEQGLNVARIGALLAGLPNTVGGVTVNRFCASGLSA
                     VQMAADRIRVGEADVMIAGGCESMSMVPMSGNKPSFNPKIFEHDENIGIAYGMGLTAE
                     KVATRWKVSREAQDAFALESHMRALKAQAAGEFGDEITPVDVIDRAPDLAARGEAGAQ
                     TQRTRTASLDEGPRPDTSLEGLAKLRPVFAAKGSVTAGNSSQTSDGAGALIVCSEAAL
                     KRFDLKPMARFVSFASRGVPPEIMGIGPIEAIPAALRYAGLKIDDMGWIELNEAFAAQ
                     SLAVIDTVGLDPAKVNPMGGAIALGHPLGATGAIRSATVIHGMRRKNLKYGMVTMCVG
                     TGQGAAGIFERM"
     gene            3577..>3830
                     /locus_tag="VFF72_03610"
     CDS             3577..>3830
                     /locus_tag="VFF72_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZV92276.1"
                     /translation="MTIRTATMNGVATIEIARPEKKNALTQAMYRQMADALDAAMADA
                     AVRAVLITGQPGIFTSGNDIDDFMKRPPGSAESPAFVFMKA"
BASE COUNT          662 a         1289 c         1328 g          551 t
ORIGIN      
        1 tgacgcgtcg ctcatcgtcc aggccaatta cgaagagcat ctggagcagc tgcgcgagtg
       61 cgacctcatc atcgaagcca tcgccgagcg catggactgg aagctcgacc tgtacaagaa
      121 gatcgcaccc ttcgtcgcgc cgcacgccat cgtcgcgtcc aacacctcgg gcctgtcgat
      181 caccaggttg tccgaggcct tgcccgatga aatcaagccg cgcttttgcg gcatccactt
      241 cttcaatccg ccgcgctaca tggcgctcgt cgagctgatc gcgacgcccg cgaccgaccc
      301 gcacgtgctc gacgacctcg aggccttcgt cacgacgacg ctcggcaagt cggtggtgcg
      361 ggccaaggac acgccgaact tcgtcgccaa ccgggtcggc atcgccggca tgctggcgac
      421 gatgaaggag gccgagacct acggcctcac ctacgacgtc gtcgacgatc tcaccggcaa
      481 gaagctcggc cgggccagtt cgggcacctt ccgcactgcc gatgtcgtcg gtctcgacac
      541 gatggcgcac gtcatcaaga cgctgcagga caacctgaag gacgatccgt tctacgccag
      601 ctacgcgacg ccgccggtgc tcgacaagct catcgccgcc ggcgccctgg gccagaagag
      661 cggcgccggc ttctacaaga aggtcggcaa ggacatcctg cgctacgagc cgaagaccgg
      721 cgagtacgtg cccggcggcg gcaaggccga cgagatcgtc gcccgcatcc tgaagaagcc
      781 gccggccgag cgattgaagc tgctgcacga ctcgaagaat ccgcaggcgc agtttctctg
      841 ggcgatcctg cgcgacagct tccactacgc cgcggtgcat ctcgccgaca tcgccgagag
      901 cgcccgcgac gtcgacttcg cgatgcgctg gggcttcggc gcgagccagg gcccgttcga
      961 gctctggcag caggccggct ggacgcaggt cgcgaagtgg gtgcaggaag acatcgacgc
     1021 cggcaaggcg ctctcgaagg cgccgctgcc gtcgtgggtg ttcgaaggtc tcggcaccgc
     1081 cgatggcggc gtgcatcgac cggagggctc gtacagcgcc gcgcacgccc gcttcgacgc
     1141 gtcgagcacg ctgcccgtct accagcgcca ggcgtttcgc gagagcgtgt tcggctccgg
     1201 cgcggccgag ccgctgaagt cgggcaccga ggtctggaag aacgcggaag tgcgcgtctg
     1261 gacgctcgac ggccaggtgc tgatcgccag catcacggcc aagctgcacc tcatcagccc
     1321 ggccgtgacc gaagggctgc tgaaggcagt cgagctcgcg gaggcgagct accagggcct
     1381 cgtgatctgg tcgcccgatg acgtcttctc cgccggcgcc aacctcgagg cgctgatgcc
     1441 ggtcttcatg cagggcgggg gcaaggcgat cgctccggag gagaagaagc tgcaggacgc
     1501 gatgctgcgg attcgctatg ccaacgtgcc ggtcgtcgcg gcggtgcgcg gcatggcgct
     1561 cggcggtggc tgcgagctgg cgatccattg cgcgcgccgc gtcgcggcca tggaaagcta
     1621 catgggcttc gtcgaggtcg gcgtcggcct gatcccgggc ggcggcgggc tcgcctacgt
     1681 cgcgcgtcgc gccgcggaaa tggcgactgc gggcggtgcc aacgcctaca tcttcgactt
     1741 cctgcgcgac gccttcacca gcgcggcgac ggccagggtc ggcaccagcg cgatcgaatc
     1801 gagaaagctc ggctacctgc tctggagcga cgtgatcgtc gcccacaagg acgagctgct
     1861 gcacgtcgcg acggcgcagg cgaaagcgat ggccgacagc ggctggcggc cgccggcgcg
     1921 cacgccgttc ccggtcgtgg gccgcagcgg catcgcgacg atcaagggcc agctcacgaa
     1981 catgaaggac ggcggcttca tcagcgccca cgactttcac gtcgccgcgc tgatcgccga
     2041 cgtcgtctgc ggcggtgacg tcgatgccgg ctcgcttgtc accgaggagt acctgatggc
     2101 gctcgagcgc aagcacttct gcggcctgct cgaccacccg aagacgcagg aacggatcat
     2161 gggcatgctg cagacgggca agcccgtcag gaattgacca ccgccaaagc ggctcacgcc
     2221 gcttctcctc aagcgggcgc cgctggcggc ccggcaatgc cggaaccgcg gcgcccgctt
     2281 gactcccgac atggagcatc gagatgagca cgaagcaagt tcaagaggcc tacatcgtcg
     2341 ccgcgacgcg cacgccgatc ggccgttcgg gccggggttt tttccgcaac acgcggcccg
     2401 acgatctgct gatcgcggcg atccatggcg cgctgaagca ggtgccttcg ctcgatccga
     2461 aggcgatcga ggacgccatc atcggctgcg ccatgcccga aggcgagcag ggcctcaacg
     2521 tggcgcgcat cggcgccttg ctcgccgggc tgccgaacac ggtgggcggc gtcaccgtca
     2581 accgcttctg cgcgtcgggc ctgagcgcgg tgcagatggc cgccgaccgg attcgcgtcg
     2641 gcgaggccga cgtgatgatc gccggcggct gcgagtcgat gagcatggtg ccgatgagcg
     2701 gcaacaagcc gtcgttcaac ccgaagatct tcgagcacga cgagaacatc ggcatcgcct
     2761 acggcatggg cctcacggcc gagaaagtgg cgacccggtg gaaggtgagc cgcgaggcgc
     2821 aggatgcctt cgcgctcgaa tcgcacatgc gcgcgctgaa ggcgcaggcg gctggcgaat
     2881 tcggcgacga gatcacgccg gtcgacgtga tcgatcgcgc gcccgatctc gcggcgcgcg
     2941 gcgaggccgg cgcgcagacg caaagaacgc gtaccgcctc gctcgacgaa ggtccgcggc
     3001 ccgatacctc gctcgagggc ctggcgaagc tgcggccggt gttcgcggcg aaggggtcgg
     3061 tcaccgccgg caacagctcg cagacgagcg acggcgccgg cgcgttgatc gtctgcagcg
     3121 aagcggcgct caagcgcttc gacctgaagc cgatggcccg cttcgtcagc ttcgccagcc
     3181 gcggcgtgcc gcccgagatc atgggcatcg gcccgatcga ggcgatcccg gcggcgttgc
     3241 gctatgccgg gctgaagatc gacgacatgg gctggatcga gctcaacgag gcgttcgcgg
     3301 cgcagtcgct cgccgtcatc gacaccgtcg gcctcgaccc ggcgaaggtc aatccgatgg
     3361 gcggcgcgat cgcgctcggc catccgctgg gcgccaccgg cgcgatccgc tcggcgacgg
     3421 tgattcacgg catgcgccgc aagaacctga agtacggcat ggtgacgatg tgcgtcggta
     3481 ccggccaggg cgcggcaggc attttcgagc ggatgtgagc gcggcgatgc aacgacgtgc
     3541 gggcatgagc gcccggacgc aacgacagga gcaggcatga ccatcaggac ggcgacgatg
     3601 aatggcgtcg cgacgatcga gatcgcgcgg cccgagaaga agaacgcgct gacccaggcg
     3661 atgtaccggc agatggccga cgcgctcgac gcggcgatgg ccgacgctgc cgtgcgcgcg
     3721 gtgctgatca ccggccagcc cggcatcttc acgtcgggca acgacatcga cgatttcatg
     3781 aagcggccgc ccggctcggc cgagtcgccg gcgttcgttt tcatgaaggc
//
LOCUS       DASRWS010000198         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2955280, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000198 DASRWS010000000
VERSION     DASRWS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955280"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /gene="pgi"
                     /locus_tag="VFF72_03615"
     CDS             <1..502
                     /gene="pgi"
                     /locus_tag="VFF72_03615"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052232.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZV92277.1"
                     /translation="HSFYQLIHQGTRLVPCDFIGFCRGLNPLGRQHDLLMSNLFAQTE
                     ALAFGKTAEEVAAEGTAAPLVPHRVFEGNRPTNALLADKLTPRTLGALVALYEHSTFV
                     QGVVWNIDSFDQWGVELGKALAVKTIPELESTSEPALHHDSSTNALIRRYRTVRDQAC
                     GDPHPT"
     gene            511..>2149
                     /gene="pgm"
                     /locus_tag="VFF72_03620"
     CDS             511..>2149
                     /gene="pgm"
                     /locus_tag="VFF72_03620"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HZV92278.1"
                     /translation="MSTRISPLAGKPAPLSLLVDVPRLVTAYYSLEPDPAEAAQRVAF
                     GTSGHRGSSFDVSFNEDHVLAITQAICDWRGKNGIDGPLFLGIDTHALSQPAAASALE
                     VLAANGVETMLAEGDEYTPTPAVSHAILVHNRGRKSGLADGIVVTPSHNPPDNGGFKY
                     NPPNGGPADAAITSAIEAAANGLLEKKLAGVRRMPYAKALAAATTHRHDFLGHYVADL
                     GSVVDLAAVREAKVRMGVDPLGGAGVHYWARIAEHYKLDLTVVSDVVDPTFRFMTVDW
                     DGQIRMDPSSPYAMQRLLDMKDRFDIAFACDTDHDRHGIVTRGDGLMQPNAYLATCID
                     YLFRHRPEWPAAAAVGKTVVSTQLIDRVAKRLGRKLYEVPVGFKWFAQGLFDGSLGFG
                     GEESAGAAFLRRDGTVWTTDKDGIVPALLSAEMTARTGKDPGRLHAALADELGRSVAD
                     RVDAPATPQQKRQLAALGADRLGASELAGEKIESVLDKAPGNGAAIGGIKVIARSGWF
                     AARPSGTEDIYKIYGESFNGPEHLKKILAQAQSVVDRA"
BASE COUNT          364 a          797 c          688 g          300 t
ORIGIN      
        1 gcactcgttc tatcagctga tccaccaggg cacgcgtctg gtgccgtgcg acttcatcgg
       61 cttttgccgg gggctgaacc cgctcggccg acagcacgac ctgctgatgt ccaacctgtt
      121 cgcgcagacc gaggcgctgg ccttcggcaa gaccgccgaa gaagtcgccg ccgaaggcac
      181 cgccgcgcct ctggtgccgc atcgcgtctt cgaaggcaac cggccgacca acgcgcttct
      241 cgccgacaag ctcacgccgc ggacgctcgg cgcgctcgtc gccctctacg aacacagcac
      301 cttcgtgcaa ggcgtcgtct ggaacatcga ttcgttcgac cagtggggcg tcgagctcgg
      361 caaggcgctg gcggtcaaga ccatcccgga gctcgagagc acgagcgagc cagcgctgca
      421 tcacgacagc tcgacgaacg cgctgatccg tcgctaccgc acggtgcgcg accaggcctg
      481 cggcgatccg cacccaacct gagcagcccc atgtccacac gcatcagtcc tctcgccggc
      541 aagccggcgc cgctgtccct tctcgtcgac gtgccccggc tcgtcaccgc ctactactcg
      601 ctcgagcccg acccggccga agccgcgcag cgcgtcgcct tcggcacgtc ggggcatcgc
      661 ggctcgtcgt tcgacgtctc gttcaacgag gatcacgtcc tcgccatcac ccaggcgatc
      721 tgcgactggc gtggcaagaa cggcatcgac ggcccgctct ttctcggcat cgacacccac
      781 gccctctcgc agccggcggc agccagcgcg ctcgaagtgc tcgccgccaa cggcgtcgag
      841 acgatgctcg ccgaaggcga cgaatacacg ccgacgccgg cggtgtcgca cgcgatcctg
      901 gtgcacaacc gcggccgcaa gagcgggctg gccgacggca tcgtcgtcac gccgtcgcac
      961 aacccgcccg acaacggcgg cttcaagtac aacccgccga acggcggccc ggccgacgcg
     1021 gcgatcacgt cggcgatcga ggcggccgcg aacggcttgc tcgagaaaaa gctcgccggg
     1081 gtcaggcgca tgccctacgc gaaggcgctg gcagcggcga cgacgcaccg ccacgacttc
     1141 ctcggccact acgtcgccga cctcggcagc gtcgtcgatc tggccgccgt ccgcgaggcg
     1201 aaggtgcgca tgggcgtcga tccgctcggg ggcgccggcg tccactactg ggcgcgcatc
     1261 gccgagcact acaagctcga cctgacggtc gtcagcgacg tcgtcgatcc gacgtttcgc
     1321 ttcatgacgg tcgactggga cggccagatc cggatggacc cgtcgtcgcc ttacgcgatg
     1381 cagcgcctgc tcgacatgaa agaccgcttc gacatcgcgt tcgcctgcga caccgaccac
     1441 gaccggcacg gcatcgtcac ccgcggcgac ggattgatgc agcccaacgc gtacctcgcc
     1501 acctgcatcg actacctgtt tcgccaccgt cccgagtggc cggcggctgc agccgtcggc
     1561 aagacggtcg tgagcacgca gctgatcgat cgcgtcgcga agcgactggg ccgcaagctc
     1621 tacgaagtgc ccgtcggctt caagtggttc gcccagggcc tgttcgacgg ctcgctcggc
     1681 ttcggcggcg aggagagcgc cggcgcggcg ttcctgcgcc gcgacggcac ggtctggacg
     1741 accgacaagg acggcatcgt gccggcgctc ctgtcggccg agatgacggc ccgcaccggc
     1801 aaggatcccg gcaggctcca cgccgcgctg gccgacgagc tcggccgctc cgtcgccgac
     1861 cgcgtcgacg cgccggcgac gccgcagcag aaaagacagc tcgcggcgct cggcgccgat
     1921 cgcctgggcg cgagcgaact cgccggcgag aagatcgaat ccgtgctcga caaggcaccc
     1981 ggcaacggcg ccgcgatcgg tggcatcaag gtcattgcca gaagcggctg gttcgcggca
     2041 cggccttccg gcaccgagga catctacaag atctacggcg agagcttcaa cggccccgag
     2101 cacctgaaga agatcctcgc ccaggctcaa tcggtcgtcg accgggcga
//
LOCUS       DASRWS010000199         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3252504, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000199 DASRWS010000000
VERSION     DASRWS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3252504"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VFF72_03625"
     CDS             complement(<1..1914)
                     /locus_tag="VFF72_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZV92279.1"
                     /translation="MPFFGRFELRPESRRLLHEGEPVPLRGRAFDVLLALWERSDRVV
                     SKSELLDLVWPGLVVEENNLAVHVSALRKHLGAQAIATVPGRGYRFTAAVAAAGPSGA
                     EGTPASAESSGSAHLVEHPRRLTNLPAALPPLYGRVDELGDLRELLGNHKLVSIVGAG
                     GMGKSRLAQTTAHAMVQRWTDGAWMIELAGVSNPALLPNVAAQCLDIKVAGGSPRDEL
                     VASLSQRSLLLVLDNCEHLLEAAASFAQAVLDGAPHVTLLMTSQEPLRLRGEQQFRIK
                     PLSVPPESTTVDARAFGAVALLEARVRSIHPRFAIGDDVMDVAVDICRCLDGLPLAIE
                     LAAARVATLGLRPVRDRLGERFRLLTGGSRSTLRRHQTLRAALEWSHSLLSHDEQAVF
                     RRLAVFAGGFAMEMAQLVAGDARLDDWAVLDHLSALVDKSLVVAGDGDSPRYRLLESA
                     RAFGLEQLAAAGETADTLRRHALAVRRHFETVDATNLDGGLRTEELEALLLPELDNLR
                     SAHGWATVDAVDVETAVVLAACSTALEDFTIESGDWLPPLQKWVEAGVEPAVAARYWR
                     AVASTNMFGHVPVTLQAEAADRARSLYLGLGLPRRAFSSVVQLAVFRLVLGDPAAART
                     AAEEARALIQEDWP"
     gene            complement(2017..>3115)
                     /locus_tag="VFF72_03630"
     CDS             complement(2017..>3115)
                     /locus_tag="VFF72_03630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZV92280.1"
                     /translation="YVAYVGSYLYMHLAYSGFGATEIWPDSPAIARFAILVGMTLFSS
                     SGLWFARGFLGLAQCAPKVDRAVAWLVRIALAAMAACVLADSPATAVDLAFGYIMLFT
                     FVMVGLGIVGIRHGREQSPIFLVASLISMAGALVTTLAVSGKLPFSALTFRAVEVGVM
                     VEASIWALALGLRLRRQQEDRAHALELARHDPLTGLYNRRGFLEQALPVYSTSARNAR
                     PVAVMMLDIDHFKLVNDQHGHDAGDRTLVAVADQLRSACRLGDIVARWGGEEFVMLLP
                     ETSAERAHGLAERLREVFAETVVAIGDGRIATFTASFGVASRTDSASLDEVLRKADAA
                     LYAAKGAGRDRVVTDTRFGEESGCALLTSDG"
BASE COUNT          509 a         1071 c         1098 g          437 t
ORIGIN      
        1 tggccagtcc tcctggatca gtgccctggc ttcttccgcg gccgtccgcg ccgccgcagg
       61 gtcgcccagc acgaggcgaa agaccgcgag ctgaaccacg ctggaaaagg ctcgccgcgg
      121 caagccgaga cccagataca acgatcgagc gcgatcggcc gcttcggcct gcagggtcac
      181 gggcacgtgg ccgaacatgt tcgtgctggc gacggcccgc caatagcgcg ccgcgacggc
      241 aggctcgacg ccggcctcga cccatttttg cagcggtggc aaccagtcgc cgctttcgat
      301 ggtgaagtcc tcgagtgcag tcgagcaggc cgcgagcacg acggcagtct cgacgtcgac
      361 ggcatcgacg gtcgcccacc cgtgcgccga ccgcaggttg tcgagctcgg gcagcagcaa
      421 ggcctcgagt tcctcggttc gcagtccgcc gtcgaggttg gtggcgtcca cggtttcgaa
      481 atgccgacgc accgccaagg cgtggcgcct cagcgtgtcg gccgtctcgc ccgcggcggc
      541 aagctgctcg agcccgaaag cccgtgccga ctcgagcagc cgatagcggg gtgagtcgcc
      601 gtcgccggca acgaccaggg acttgtcgac aagagcgctc aggtggtcga gcaccgccca
      661 atcgtcgagc cgtgcgtcgc cggcgacgag ctgcgccatt tccatggcga atccgccggc
      721 gaatacagcg aggcggcgaa acaccgcctg ctcgtcatgg ctcagaaggc tgtggctcca
      781 ctcgagcgcg gcgcgcagtg tctggtgccg ccgcaaagtc gagcgtgatc cgccggtcag
      841 gagtcgaaag cgttcgccga gcctgtcgcg cacggggcgc aggcccagcg tggcgacgcg
      901 cgccgctgcg agctcgatgg ccaggggcag gccgtcgagg cagcggcaga tatcgacggc
      961 gacgtccatg acgtcgtcgc cgatggcgaa tcgcgggtgg atcgaacgca ccctcgcttc
     1021 aaggagagcc accgcgccga aggcacgcgc gtcgaccgtg gtggattcgg gcggcaccga
     1081 caacggcttg atccgaaatt gctgctcccc tctcaggcgt agcggctcct ggctggtcat
     1141 cagcagcgtg acgtggggcg ctccatccag aaccgcctgc gcgaacgagg ctgccgcctc
     1201 gagcaggtgt tcgcagttgt cgagcaccag cagcaggctt cgctgcgaaa ggctggcgac
     1261 cagctcgtct cgaggagaac cgcccgcaac cttgatgtcg aggcactgcg ccgcgacgtt
     1321 cggcagcagc gccggattgg acacaccggc cagctcgatc atccatgccc cgtcggtcca
     1381 gcgctggacc atcgcatgcg cggtcgtctg cgccagtcgg ctcttgccca tgcctcctgc
     1441 gccaacgatg ctgacgagct tgtggttgcc gagcagctcg cgcaggtcgc cgagctcgtc
     1501 gactcgcccg tagagcggtg gcagcgccgc tggtagattg gtcagccgcc ttggatgctc
     1561 gacgaggtgg gcggaccccg aagactcggc tgacgccggc gttccttcgg cgccggaagg
     1621 gccggcggcg gccaccgccg cggtgaatcg gtagccccgg ccgggcaccg tcgcgatggc
     1681 ctgggcgccg agatgcttgc gcagcgcgct gacgtgcacg gcgaggttgt tctcctcgac
     1741 gacgagaccg ggccacacga gatcgagcag ctcgctcttg gagaccaccc ggtcgcttcg
     1801 ctcccacagc gcgagcagga cgtcgaatgc gcgcccgcgc agcggcaccg gctcgccctc
     1861 atgcagcagg cggcggctct cgggtcgcaa ctcgaagcgt ccgaagaacg gcactggcga
     1921 ctcccaggcg cctccgttcg gcctcgcagg acgtcgagga ggtgatcctg gattctagcg
     1981 agccgcgtca cgccgcccgg ggctggccgc ggctgctcag ccgtcgctgg tcaggagcgc
     2041 gcaaccgctc tcttcaccga agcgggtgtc ggtgacgacc cggtcccggc cggcgccctt
     2101 ggcggcatag agcgccgcgt cggcctttct gagcacttcg tcgaggctcg ccgaatcggt
     2161 tcgcgaggcg acgccgaagc tggccgtgaa agtcgcgatg cggccgtcgc cgatcgccac
     2221 cacggtctcg gcgaagacct cgcgcagccg ttcggccagc ccgtgcgcac gctcggccga
     2281 ggtttccggc agcagcatca cgaactcctc gccgccccag cgggcgacga tgtcaccgag
     2341 ccggcaggcg ctgcgcagct ggtcggccac ggcgaccagc gtacggtcgc cggcgtcgtg
     2401 cccgtgctgg tcgttgacga gcttgaaatg gtcgatgtcg agcatcatca ccgccaccgg
     2461 ccgggcgttg cgggccgacg tgctgtagac ggggagcgcc tgttccagga agccgcggcg
     2521 gttgtagagg cccgtgagcg gatcgtgtcg cgcgagctcg agggcgtggg cacggtcttc
     2581 ctgctggcgg cgcaggcgca ggccgagcgc cagcgcccag atcgaagcct cgaccatcac
     2641 gcccacctcg acggcgcgaa acgtgagggc gctgaacggc agcttgcccg acacggccag
     2701 cgtcgtcacg agcgcgccgg ccatgctgat gagcgaggcg acgagaaaga tcggcgactg
     2761 ctcgcggcca tggcggatgc cgacgatgcc caggcccacc atcacgaagg tgaacagcat
     2821 gatgtagccg aacgccaggt cgaccgccgt cgccggcgaa tcggcgagca cgcaggccgc
     2881 catcgccgcg agcgcgatgc ggaccagcca ggcgacggcg cggtcgacct tcggcgcgca
     2941 ttgcgccagg ccgaggaagc cgcgcgcgaa ccagagaccg ctgctcgaga acaacgtcat
     3001 gccgacgagg atcgcgaagc gggcgatcgc cggcgagtcg ggccagatct cggtcgcgcc
     3061 gaagcccgag taggcgagat gcatgtagag atagctgccg acgtaggcga cgtag
//
LOCUS       DASRWS010000200         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4108698, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000200 DASRWS010000000
VERSION     DASRWS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4108698"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFF72_03635"
     CDS             complement(<1..304)
                     /locus_tag="VFF72_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92281.1"
                     /translation="MSRILVVEDEPAIAELIAINLRHAAFDVTLAEDAAQAQAAVDQA
                     LPDLVILDWMLPGKSGHALAREWRAQPRTRELPIIKLTARADEADKVSALDAGADDY"
     gene            complement(321..1031)
                     /gene="phoU"
                     /locus_tag="VFF72_03640"
     CDS             complement(321..1031)
                     /gene="phoU"
                     /locus_tag="VFF72_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZV92282.1"
                     /translation="MSNDRHLSTQFDAELSGISSRVLEMGGMVESQVARAVQALVTFS
                     GDVASEVLATEERVNQTEIEIDLDLSTIIARRQPTARDLRLLIAISKTIANLERVGDE
                     AARIARTVQRLVDSGVSTRLRLPVNDLAYEAELAVAQLRKALDAFARLDVVRAVEVLK
                     RDDLIDQEFEGLLRKLITFMMEDARTISSSIDLIFVAKAIERVGDHAKNLAEQIIYIV
                     KGTDVRHNPVVRVESLVR"
     gene            complement(1042..1839)
                     /gene="pstB"
                     /locus_tag="VFF72_03645"
     CDS             complement(1042..1839)
                     /gene="pstB"
                     /locus_tag="VFF72_03645"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZV92283.1"
                     /translation="MDTRVDKSAATAITGTPKFSIRNLNFYYGKFHALKGISLDIPEN
                     KVTAFIGPSGCGKSTLLRTFNRMFELYPEQRAEGEIILDGENILASKEDASLIRARVG
                     MVFQKPTPFPMSIYDNVAYGIRLFESLPRVEMDERVEWALRKAALWTEVKDKLAQNGA
                     GLSGGQQQRLCIARGIAIKPEVLLLDEPCSALDPISTGKIEELIHELKSDYTVVIVTH
                     NLQQAARSSDFTAYMYLGDLIEFGRTQDLFMRPTKRDTEDYITGRFG"
     gene            complement(1851..2738)
                     /gene="pstA"
                     /locus_tag="VFF72_03650"
     CDS             complement(1851..2738)
                     /gene="pstA"
                     /locus_tag="VFF72_03650"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZV92284.1"
                     /translation="MSTAPKGTVSTAAQVARAARHAARKRVNAIALSLSLAAMAFGLF
                     WLLWILEETIRLGIGGMSLSLFTQSTPPPQAPTGGLANAIFGSLSMVAVATLIGTPIG
                     ILAGIYLAEYGTKSRLGHVTRFMNDILLAAPSIVIGLFIYSIVVVPTRTFSGWAGALA
                     LSLIVIPIVVRTTENMLRLIPHTLREAAFALGAPKWRVIMSITLKAARAGVITGVLLA
                     LARVAGETAPLLFTALSNQFWSSDLGGPTASLPVTIFKFAMSPYENWQKLAWAGVFVI
                     TAGVLALNILARVLVRTKP"
     gene            complement(2735..3775)
                     /gene="pstC"
                     /locus_tag="VFF72_03655"
     CDS             complement(2735..3775)
                     /gene="pstC"
                     /locus_tag="VFF72_03655"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HZV92285.1"
                     /translation="MIRQADLVATRPADAFPDAGSTARIVPKGRPAAIRRARPWADVL
                     FSATVHLAAWVTLAILVGIVLSLVVGALPAIEQFGLGFLTSAQWDPVQDKYGGLVMIY
                     GTLMTSFIALLIAVPVSFGIALFLTELSPAWLKRPLGTAVELLAAIPSIVYGMWGLLV
                     FSPVLSSYVQQPLEKTFAGMPVLGALFSGPPVGIGLLSAGIILAIMVIPFIASVMRDV
                     FEVTPPLLKESAYGLGSTTWEVMIKVVLPYTKTGVIGGIMLGLGRALGETMAVTFVIG
                     NFNQLDSLSLFEAANSITSALANEFAEAGDGLHKAALMYLALVLFFITFVVLGGSKLL
                     LARLGKREGARS"
     gene            complement(3797..4834)
                     /gene="pstS"
                     /locus_tag="VFF72_03660"
     CDS             complement(3797..4834)
                     /gene="pstS"
                     /locus_tag="VFF72_03660"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZV92286.1"
                     /translation="MNQLFVRSLAGAVLAASAGVSQAQDMTGAGSSFAAPIYSKWADS
                     YHNATGARLNYQSIGSGAGLQQIRARTVDFGASDMPLSDEQLAKDHLVQFPTVIGAVV
                     PVVNIKGVKPGEMKLTGAVLGDIYLGKITQWNDPAIVGLNPGVPLPDAAISTVRRADG
                     SGTTFIFTNYLSKVNPEWKTKVGEGTAVNWPKGTGGKGNEGVASFVQRLPDSIGYVEY
                     AYARQNKMAYVTMKNKAGNFVAPDADNVKAAAADADWSKSFHQLLTDQPGRQAWPISG
                     ATFVLMHRAQDKPARASNTLKFFDWALANGDKAAADLDYVTLPQAVKSMIRKEWSDDI
                     RDSDGKSIAFR"
     gene            5136..>5664
                     /locus_tag="VFF72_03665"
     CDS             5136..>5664
                     /locus_tag="VFF72_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZV92287.1"
                     /translation="MTDPTSIPHRRTALPADDRPLWRVFVRFLGPMVVGNVLQALTGT
                     FNSVFVGQMLGTKGYAAAAAVFPIVFFFVSLVIGIGSGGSVLIGQAWGAREPHRVKAI
                     AGTAIMLGLAVGALVALFGGLFTEPMLRALRTPPDVLPVSIGYARVMMLAMPGLLVFI
                     VSTQLLRGVGDTVTPL"
BASE COUNT          933 a         1821 c         1925 g          985 t
ORIGIN      
        1 gatagtcgtc ggcgccggcg tcgagggccg acaccttgtc ggcttcatcg gcgcgggcgg
       61 tgagcttgat gatcggaagc tctcgggttc gtggctgcgc gcgccattcg cgcgccagcg
      121 catggccgct cttgccgggc agcatccagt cgaggatcac gagatcgggc agcgcctggt
      181 cgacggcggc ctgcgcctgc gcggcgtctt cagccagcgt cacgtcgaac gccgcatgac
      241 gcaggttgat cgcgatcagc tcggcgatgg ccggctcgtc ctcgacgacc aaaattcggc
      301 tcatctcccg ggccctccgt tcagcgaacg aggctttcga ccctcaccac ggggttgtgg
      361 cgaacgtcgg tacccttgac gatatagatg atctgctcgg ccaggttctt ggcgtgatcg
      421 ccgacccgct cgatcgcctt ggcgacgaag atcaggtcga tgctcgagga aatcgtccgc
      481 gcgtcttcca tcatgaaggt gatgagcttt cgcagcaggc cctcgaactc ctggtcgatc
      541 aggtcgtcgc gcttgagcac ctcgacggcg cgaacgacgt cgagccgcgc gaaggcgtcg
      601 agcgccttcc tcagttgcgc gacggcgagc tcggcttcat aggcgaggtc gttcaccggc
      661 aggcgaagac gtgtcgagac gccggagtcg accagccgct gcaccgtgcg cgcgatccgc
      721 gctgcttcgt cgccgacgcg ctcgaggttg gcgatggtct tcgagatggc gatgagtagg
      781 cgcaggtcgc gcgccgtggg ctggcgtcgg gcgatgatgg tcgacaggtc gaggtcgatc
      841 tcgatctcgg tctggttgac gcgctcttcg gtggcgagca cctccgaggc gacgtcgccg
      901 ctgaaggtga caagcgcctg gacggcgcgt gccacttgcg actccaccat gccccccatc
      961 tcgagcacgc gcgacgagat gccgctcagc tcggcgtcga attgggtcga aagatgacga
     1021 tcgttgctca cgggatttcc tttagccgaa gcgaccggtg atgtagtcct cggtgtctct
     1081 cttcgtcggt cgcatgaaca ggtcctgcgt ccggccgaac tcgatcaggt cgcccaggta
     1141 catgtaggcg gtgaagtcgg agctgcgcgc cgcctgctgc aggttgtggg tgacgatgac
     1201 caccgtgtag tcgctcttca gctcgtggat cagctcctcg atcttgccgg tggaaatcgg
     1261 gtcgagggcc gagcagggct cgtcgagcag caggacttcc ggcttgatgg caatgccgcg
     1321 agcgatgcac agccgctgct gctgcccgcc ggacaggccc gcgccatttt gcgcgagctt
     1381 gtctttgacc tcggtccaca gggccgcctt cctgagcgcc cattcgacac gctcgtccat
     1441 ctcgacgcgg ggcagcgatt cgaagagcct gatgccgtag gcgacgttgt cgtagatcga
     1501 catcgggaaa ggcgtcggct tctggaacac catgccgacc ctggcacgaa tcagcgaggc
     1561 atcctccttc gaagccagga tgttttcgcc gtcgagaatg atctcgcctt cggcgcgctg
     1621 ctcggggtag agctcgaaca tccggttgaa ggtgcgcagc agcgtcgact tgccgcagcc
     1681 ggacggaccg atgaatgcgg tcaccttgtt ctccgggata tcgaggctga tgcctttcag
     1741 cgcatggaac ttcccgtagt agaagttcag gttccggatc gagaacttcg gcgtcccggt
     1801 gatcgccgtc gcggccgact tgtcgactcg tgtgtccatg acgttcctcg tcaaggcttc
     1861 gtgcgcacca gcacgcgggc cagaatgttg agcgccagca cgccggcggt gatcacgaag
     1921 acgccggccc aggcgagctt ttgccagttc tcgtacgggc tcatcgcgaa cttgaagatc
     1981 gtcacgggca ggctggcggt cggaccgccg aggtcgctgc tccagaactg gttcgacaag
     2041 gcggtgaaga gcagcggcgc cgtttcgccg gcaacgcgcg cgagcgcgag caggacgccg
     2101 gtgatgacac cggcacgcgc cgccttcagc gtgatgctca tgatcacgcg ccacttcggc
     2161 gcgccaagcg caaaggccgc ctcgcgcagc gtgtggggaa tgagcctgag catgttctcg
     2221 gtcgtccgga cgacgatcgg gatgacgatc agggacaacg cgagcgcccc cgcccacccg
     2281 gaaaacgtcc tcgtgggcac gacgacgatg ctgtagatga acaggccgat gacgatcgac
     2341 ggcgccgcca gcaagatgtc gttcatgaaa cgggtgacgt gcccgagccg gcttttcgtt
     2401 ccgtattcag cgaggtagat gccggcgagg attccgatcg gcgtgccgat cagcgtcgcc
     2461 acggcgacca tcgagagcga cccaaagatc gcgttggcga ggccgccggt cggtgcctgc
     2521 ggcggcggcg tcgactgggt gaacagcgac aggctcatgc ccccgattcc caggcggatg
     2581 gtttcctcga ggatccagag cagccagaag aggccgaagg ccatcgccgc cagcgacagc
     2641 gacagggcga tggcgttgac gcgcttgcgc gccgcatgcc tggccgcacg agccacctgc
     2701 gccgcggtcg aaacagtgcc cttcggcgcc gtgctcatga tcgcgccccc tcgcgcttgc
     2761 cgagcctggc cagcagcagc ttcgagccgc ccagcaccac gaaggtgatg aagaagagga
     2821 ccagcgcgag atacatcagg gcggccttgt gcaggccgtc gccggcctcg gcgaactcgt
     2881 tggccagcgc cgacgtgatg ctgttggccg cctcgaacag cgacagcgaa tccagctggt
     2941 tgaagttgcc gatcacgaag gtgacggcca tcgtctcgcc gagcgcgcgg ccgaggccga
     3001 gcatgatgcc gccgatgacg ccggtcttgg tgtagggcag gacgaccttg atcatcacct
     3061 cccacgtggt cgatccgagc ccgtacgcgg actccttcag cagcggcggg gtgacctcga
     3121 aaacgtcgcg catgaccgag gcgatgaagg ggatgaccat gatggccagg atgatccccg
     3181 cggaaaggag cccgatgccc acgggcggcc cggagaaaag cgcgcccaga accggcatgc
     3241 cggcgaaggt cttctcgagc ggctgctgga cgtagctcga cagcaccggg ctgaacacca
     3301 gcaggcccca catgccgtag acgatggacg gaatggcggc caggagctcg accgccgtac
     3361 cgagcgggcg cttcagccat gccggcgaga gctcggtcag gaacagggcg atgccgaagc
     3421 tgaccggcac cgcgatcagc agtgcgatga acgacgtcat cagcgtgccg tagatcatca
     3481 ccaggccgcc gtatttgtcc tgcaccggat cccactgtgc gctggtcagg aacccgagcc
     3541 cgaactgctc gatggccggc agggcgccga cgacgagcga aagcacgatg ccgacgagga
     3601 tcgcgagcgt cacccaggcc gcgaggtgga ccgtcgccga aaacagcacg tcggcccagg
     3661 gccgagcgcg ccggatcgcg gcaggacggc ccttcgggac gatgcgggcc gtgcttccgg
     3721 cgtccgggaa cgcgtccgcg ggacgcgtgg cgacgaggtc ggcttgtctg atcatcgacg
     3781 aggagttgcc actcagttac ctgaaggcga tcgacttgcc gtccgagtcg cggatgtcgt
     3841 cggaccactc cttgcggatc atcgacttca ccgcctgggg cagcgtcacg tagtcgaggt
     3901 cggccgccgc tttgtcgccg ttggccagcg cccagtcgaa gaacttcagt gtgttgctgg
     3961 cgcgagcggg cttgtcctgg gctcggtgca tcaggacgaa ggtcgcgccg ctgatcggcc
     4021 aggcttgcct tcccggctga tcggtcagca actggtggaa gctcttcgac cagtcggcat
     4081 cggcggcagc cgccttgaca ttgtccgcat cgggcgcgac gaaattgccg gccttgttct
     4141 tcatggtcac gtacgccatc ttgttctgcc tggcataggc gtattcaacg tagccgatgg
     4201 aatccggcag ccgctggacg aacgacgcga cgccctcgtt gcccttgccg ccggtaccct
     4261 tgggccagtt gacggccgtg ccctcgccca ccttcgtctt ccattcgggg ttcaccttgg
     4321 acaggtagtt ggtgaagatg aaggtcgtgc ccgatccgtc ggcccggcgg accgtcgaga
     4381 tcgcggcgtc gggaagagga acgccggggt tgaggccgac gatcgccgga tcgttccatt
     4441 gggtgatctt gccgaggtag atgtcgccga ggaccgcgcc ggtgagcttc atctcacccg
     4501 gcttgacgcc cttgatgttc accaccggca cgaccgcgcc gatcacggtc ggaaactgca
     4561 ccaggtggtc ctttgcgagt tgctcgtcgc tgagcggcat gtccgacgcg ccgaagtcga
     4621 cggtccgggc gcggatctgc tgcagaccgg cgcccgagcc gatcgactgg tagttcaggc
     4681 gagcgccggt cgcgttgtgg tacgagtcgg cccacttcga atagatcggg gcggcgaagc
     4741 tcgagccggc tccggtcatg tcctgggcct gcgagacgcc agcggaagcg gcgagcaccg
     4801 caccggcgag cgatcggacg aagagctgat tcatgcgcga gcaccttccg gcagagccga
     4861 agtcaatcgg atgcgctcac tctagaaagg ctttgtgacg ccttcgtgac tgcagcagtg
     4921 gcgcggcgag cgatggcggg tgcccgccag atccggctat cggtctcgcg tgcgacgagt
     4981 cggaccgagg gtcggccgac acccggtccg accaggcgaa acgatgcgtc gggtcggcgc
     5041 gacacgatgt gccgggccgg tcgggctcat cgcctgttgc gcggacaatc cgcccttccc
     5101 cgggccgagc cgcggcgtgg gcttccacgt ccgacatgac cgacccgaca tcgattccgc
     5161 accgtcgcac cgcgctgccc gccgacgacc ggccgctctg gcgcgtcttc gtgcgcttcc
     5221 tcggcccgat ggtcgtcggc aacgtgctgc aggcgctcac cggaacgttc aacagcgtct
     5281 tcgtcggcca gatgctcggc accaagggct atgccgcggc ggcggcggtc tttccgatcg
     5341 tgttcttctt cgtttcgctg gtgatcggca tcgggtccgg cggctcggtg ctcatcggcc
     5401 aggcgtgggg cgcacgcgag ccgcaccggg tcaaggcgat cgccggcacg gcgatcatgc
     5461 tgggactggc cgtcggtgcg ctggttgccc tcttcggggg cctcttcacc gagccgatgc
     5521 tgcgcgcgct gcgcacgccg cccgacgttc ttcccgtgtc gatcggctat gcacgagtca
     5581 tgatgctggc gatgccgggc ctgctggtgt tcatcgtcag cacgcaactg ctgcgcggcg
     5641 tcggcgatac cgtgacgccg ctct
//
LOCUS       DASRWS010000201         8869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_655115, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000201 DASRWS010000000
VERSION     DASRWS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655115"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFF72_03670"
     CDS             complement(<1..433)
                     /locus_tag="VFF72_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HZV92288.1"
                     /translation="MKLSDFILRDMEVILAQWEAFARTMPLSSHMDSLALRDHAEQIL
                     VAICKDIDLAQSVEEQSRKSRGLAAPLDARETAAQTHALLRARGGFDINQMASEYRAL
                     RASVLSLWAAACGPGGTDLQQTIRFNGAIDQALCESIAFFSA"
     gene            complement(526..1314)
                     /locus_tag="VFF72_03675"
     CDS             complement(526..1314)
                     /locus_tag="VFF72_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HZV92289.1"
                     /translation="MDAGERTGTRGGARRFAAPITAPFDLGNALSTCGHAVLDPRGFA
                     TFVSTPAAELEPLRASWSDLPPDNYLRDGGRYRRRRHSCFVVRGASVEPVAHRAHWQP
                     VEYNALHGGIERWFEPIEPAVIAQPSWTRTLRAMARAASDLRGEQPWYVEAHQFRIDT
                     SDGIGRPTPEGAHRDGVDLVAVVLVDRAGVKGGETRVFDADGPTGLRFTMEEPWTCLL
                     LDDARVIHESTPIQPIGEFGHRDTLVLTLRAGAFQGPAVVPPRA"
     gene            complement(1376..2407)
                     /locus_tag="VFF72_03680"
     CDS             complement(1376..2407)
                     /locus_tag="VFF72_03680"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZV92290.1"
                     /translation="MFETATAGSLPKPSWLSETVKLWPQWAASGAELDRAKADATLLW
                     LKVQEDAGVDVVGDGEQSRQHFVHGFLARVEGIDFEHKVKMGIRDNRYDAMVPQVTGP
                     LRLAGRVHETEARLVRGHTARRMKFTLPGPMTIVDTVADRHYGQGRDGRIRMAFAFAD
                     LLNQEARALEADGVDVIQFDEPAFNVYMDDAADWGVEALERAAAGLKCKTAVHICYGY
                     GIKANIDWKAGLGQEWRQYERVFPALAKSAIDQVSLECLHSHVPPELMRLLDGKDVMV
                     GVIDVASDVVETPQEIAETIGTALRFVPRERLIACTNCGMAPMRREIAEAKLVALVQG
                     AELARQRFA"
     gene            2467..3708
                     /locus_tag="VFF72_03685"
     CDS             2467..3708
                     /locus_tag="VFF72_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HZV92291.1"
                     /translation="MRDDTEAATSAGGPLPATRRIEVLDVLRGFALLGIFIMNMPSFA
                     HSIFTPPPAQDATLSGSVVFLRELLFAGKFNLIFGMVFGIGFTLQLRRLEQTRPAQAT
                     FIYMRRLAVLLVIGLVHAAFFWMGDVLVAYAVLGLGLLAIRRAPDAVVLALLGACLVF
                     PAVADAMSTHLFSDETQMLSTFEFYQFASTNDVAFGQGGFLDAVRETMRVFAWAYASP
                     MGLFTVGSFFVQMATGILAGFLVGRRGWIERLPALAPRLPAAQWIALGVVLVAAASWW
                     LVGGAAVADGSEDTAASFARTIGRAALMCFYAVTLVRLALAPATARFFRIFALPGRMP
                     LTNYLLQTAMGLAIFYGWGLGLWGRTTPLEEMALAVGLYALVQLPLSALWLGRFRYGP
                     IEYVWRRLTYGSLPRRALQRA"
     gene            complement(3741..4736)
                     /locus_tag="VFF72_03690"
     CDS             complement(3741..4736)
                     /locus_tag="VFF72_03690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZV92292.1"
                     /translation="MRLSVLDQSVSIAGSSEDAAIRDTVSLAEDCEKLGYSRFWVSEH
                     HGLPTIVGSAPEIVMAAIAARTSSIRIGSAGIMLPHYSALKVAEQFRVLEALAPGRID
                     LGLGRAPGGDMRTARALNPNASHAAEDFPIQVRDLHAWTSLGTHEGITAHPLGPHAPE
                     IWILGSSDYGAQLAAHFGLPYAFAYFFTDGRGADEAMALYRARYRPSERHPQPQSTLC
                     IWALVADTDAAASHLALSRDRWRIDRQRGRLGPLQSPDEIAASGFDSAEEAELASMRA
                     RSFVGSRANVGAKIRELARDFSLDEVVVNTWAHDPAVRRRSYGLLAEEFGLVRRD"
     gene            complement(4824..5312)
                     /locus_tag="VFF72_03695"
     CDS             complement(4824..5312)
                     /locus_tag="VFF72_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZV92293.1"
                     /translation="MSHDESHPSFAEAPWKHDGVRVIAAGQLDPNTAQTPGMNRKAAI
                     TFARVGAQKLWAGTVHIHADAKTGAHHHGHLESVIYVVKGRARMRWGNALEFTAEAGP
                     GDFIYVPPFVPHQEINASATETLECVLCRSDGEAVAVNLDIEPVEKPDTVRWIDPTHP
                     RG"
     gene            5497..5709
                     /locus_tag="VFF72_03700"
     CDS             5497..5709
                     /locus_tag="VFF72_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZV92294.1"
                     /translation="MTTQTGTVKWFNEAKGFGFIAQDAGGADLFAHFRDIQGNGFKIL
                     AENQRVEFEVKQGQKGLQAANIRVLA"
     gene            5864..6901
                     /locus_tag="VFF72_03705"
     CDS             5864..6901
                     /locus_tag="VFF72_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZV92295.1"
                     /translation="MASPIGETNHQVRLARRPVGLPTRADWSFTAETLTDADEGGVLV
                     KTLALSLDPAMRGWMNEGKSYIAPVGIDEVMRAGGIGTIVSSRSPKFAVGDRVSGPLG
                     VQEFARFDAAQLARSALQRIDLSAGSMTQWLNVLGMPGMTAYFGLLDVGQPKSGETVV
                     VSGAAGAVGQTVGQLAKIKGCRAVGIAGGPAKCKWVVETLGFDACIDYKAGAVKDGLK
                     AHCPDGVDVYFDNVGGEILDTVLTRLARKARIVICGAISQYNNTDAVKGPANYLSLLV
                     HRARMEGMVVFDYADRFPVAIAELAGYLRDGRLKSREDVVSGIEAFPEALLRLFRGEN
                     FGKLVLKVAEG"
     gene            6982..>8869
                     /locus_tag="VFF72_03710"
     CDS             6982..>8869
                     /locus_tag="VFF72_03710"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZV92296.1"
                     /translation="MSAPPNSRLTDDLSDDGPPAEVLRAFVAQSLELLVATDRDGLIR
                     WSNERFSSATGLVADGRANLLQAIAGGSAGETTRRRLQAGLDDDGIDATGLRLAGSNG
                     ADLWVDARARRVGDQVLWTFTDVTSTRFLANQARRQGELLDTAQEFGRLGVWERKIPS
                     GEGRWDRHVFGFWGLDPAGGTPSFVDAIERIHPEDREKMNYLESTRRAGRYSQRYRVI
                     HPDGRTRWIHSQWEVKNGPAGVPDRAFGVMMDDTEAYEAARALVDVSAQLKMAVELGN
                     IAIWRHDLRTQRMYYSNRAFDLLQMPTRSEGLSIDEVRALIHPEDIPGVIESAERALK
                     SDRPTDMEARYRRKDGSYRYVMTRRVVERDAAGQPIAFVGVALDVTEAVLHRRHADAL
                     ARRIEAASKAAGIGLWTTSDKPPHTDWNTQTFAIFDVDPPVVPDFSHWLRRCVHPDDR
                     TRVGNAAHAYLAHGDGPFEIEFRTVRRDGGVRWVVMRADRDRESTDQRRFLGVALDVT
                     DHHAAVDALRAASERATLIASYAGIGMWEGMLDGSPVRWDAQMFHLRGLSPEAAVPSR
                     DERLARVHPDDRQQLLDSLPEVEESARATSYEFRWKMPDGSWRWLASRSALVRDAAGV
                     ALRRV"
BASE COUNT         1333 a         3093 c         3023 g         1420 t
ORIGIN      
        1 cggcgctgaa aaacgcgatc gattcgcaca gggcctggtc gatcgctccg ttgaaccgga
       61 tcgtctgctg cagatccgtg ccgcccggtc cgcacgctgc cgcccagagg ctcaggacgc
      121 tcgcacgcaa cgcccgatac tcggaggcca tctggttgat gtcgaagccg ccgcgggccc
      181 gaagcagggc gtgcgtctgc gctgccgtct cccgggcatc gagcggagcc gccaggcccc
      241 gggacttcct ggactgttcc tcgaccgact gcgcgagatc gatgtccttg cagatggcga
      301 ccaggatctg ctcggcgtga tctcgcagcg ccagcgagtc catgtgcgac gacagaggca
      361 tcgttcgcgc gaacgcttcc cactgcgcga ggatcacctc catgtcgcgg agaatgaaat
      421 cggacagctt catcggcggc tccatgagcg agaagaactt gctcaggcca ggctggcggg
      481 tgccggggag aagcgtcgcg attatcgtcc cacccccggg aggggtcagg cccgcggcgg
      541 gacgacggcc gggccctgga aggcgcccgc acgcagggtc aggaccagcg tgtcgcgatg
      601 cccgaattca ccgatcggct ggatcggcgt gctttcgtgg atgacgcgcg cgtcgtcgag
      661 cagaaggcag gtccagggct cttccatcgt gaatcgcagg ccggtcgggc cgtcggcgtc
      721 gaagacacgc gtctcgccgc ccttgacgcc cgcgcggtcg acgaggacga cagcgacgag
      781 gtcgacaccg tcgcgatgcg ccccttccgg cgtcggccgg ccgatgccgt ctgacgtgtc
      841 gatgcgaaac tggtgcgcct cgacatacca aggctgctcg ccgcgcagat ccgacgccgc
      901 ccgcgccatc gcccgcagcg tcctggtcca cgacggctgg gcgatgaccg ccggctcgat
      961 cggctcgaac cagcgttcga tgccaccgtg cagcgcgttg tattcgaccg gctgccagtg
     1021 ggcccggtgg gccaccggct cgaccgacgc gccgcgcacg acgaagcacg aatggcggcg
     1081 acgccggtag cggccgccgt cgcgcaggta gttgtcgggc ggcaggtcgc tccagcttgc
     1141 acgcaagggt tcgagctcgg ccgccggcgt cgagacgaag gtcgcgaagc cccggggatc
     1201 gagcacggcg tgtccgcagg tcgacaaggc gttgccgagg tcgaagggcg ccgtgatcgg
     1261 tgcagcgaat cggcgagcac cgcccctcgt ccccgtcctt tcgccggcgt ccatgccttc
     1321 gcgcacggtc gtttccgctc cgggaatctt gccggatcgc tcgctcatgc ccggatcagg
     1381 cgaagcgctg gcgcgccagc tccgcgcctt gcaccagggc gacgagcttg gcctcggcga
     1441 tttcgcgacg catcggcgcc atgccgcaat tggtgcaggc gatgagccgc tcgcgcggga
     1501 cgaaacgaag cgcggtgccg atcgtctcgg cgatctcctg cggcgtctcg accacgtcgg
     1561 aggcgacgtc gatgacgccc accatcacgt ccttgccgtc gagcaggcgc atcagctccg
     1621 gcggcacatg cgaatgcagg cattcgagcg acacctggtc gatcgcgctt ttcgccagcg
     1681 cgggaaagac gcgctcgtac tggcgccact cctggccgag ccccgccttc cagtcgatgt
     1741 tggccttgat gccgtagcca tagcagatgt gcacggcggt cttgcacttc aggccggcgg
     1801 ccgcccgctc gagcgcctcg acgccccaat cggcggcgtc gtccatgtag acgttgaagg
     1861 ccggctcgtc gaactggatg acgtcgacgc cgtcggcctc gagcgctcgc gcctcctggt
     1921 tgagaaggtc ggcgaaggcg aacgccatgc ggatgcgtcc gtcccggccc tggccatagt
     1981 ggcgatcggc gacggtatcg acgatcgtca tcgggccggg cagcgtgaac ttcatccgcc
     2041 gcgcggtatg gccgcggacg agtcgcgcct cggtctcgtg cacgcgcccg gcgaggcgca
     2101 acggcccggt gacctgcggc accatcgcgt cgtagcggtt gtcgcggatt cccatcttca
     2161 ccttgtgctc gaagtcgatg ccttcgacgc gggcgagaaa gccgtgcacg aagtgctggc
     2221 gcgactgctc gccgtcgccg acgacgtcga cgccggcgtc ttcctggacc ttgagccaga
     2281 gcagcgtcgc atcggccttg gcccgatcga gctcggcacc gctggccgcc cactgcggcc
     2341 acagtttgac ggtctcggac agccacgacg gtttgggcag gctgccggcg gtggcggtct
     2401 cgaacatctc acgtttcctt ttgaaagcgg cacgccggcg atgcccgcct ttgctaccgt
     2461 tgcgccgtgc gagacgacac cgaagccgcg acatccgcag gcgggcccct gcccgcgaca
     2521 cggcggatcg aggtgctgga cgtgcttcgc ggcttcgcgc tgctcggcat cttcattatg
     2581 aacatgccga gcttcgcgca ctcgatcttc acgccgccgc cggcacagga cgcgaccctc
     2641 tccggcagcg tcgtcttcct gcgcgagctg ctgttcgccg gcaagttcaa cctgatattc
     2701 ggcatggtct tcggcatcgg cttcaccttg cagctgcgcc gcctcgagca gacccgcccg
     2761 gcgcaggcca ccttcatcta catgcgccgg ctggccgtgc tgctcgtcat cggcctcgtc
     2821 cacgccgcct ttttctggat gggcgacgtg ctcgtcgcct acgccgtgct cggcctgggg
     2881 ctgctggcga tccgccgcgc gccggacgcc gtggtgctgg cgctgctcgg cgcatgcctc
     2941 gtctttccgg cggtcgccga cgcgatgtcg acgcatctct tctcggacga gacgcagatg
     3001 ctctcgacct tcgagttcta ccagttcgca agcaccaacg acgtcgcctt cggccagggc
     3061 ggctttctcg atgccgtgcg cgagacgatg cgcgtgttcg cctgggccta cgcctcgccg
     3121 atggggctgt tcacggtcgg cagcttcttc gtacagatgg ccaccggcat cctggccggc
     3181 tttctcgtcg gccgccgggg ctggatcgaa cggctgccgg cgctggcacc gcggctgccg
     3241 gccgcgcagt ggatcgcgct cggggtcgtg ctggtcgccg ccgccagctg gtggctcgtc
     3301 ggcggcgcgg ccgttgccga cggcagtgag gacaccgccg cctcgttcgc gcgaacgatc
     3361 ggccgcgccg cgctgatgtg cttctatgcc gtgacgctgg ttcgcctggc gctcgccccg
     3421 gcgaccgccc gcttcttccg gatcttcgcg ctgcccggac gcatgccgct cacgaactac
     3481 ctgctgcaga cggccatggg actggccatc ttctacggct ggggcctggg cctgtggggc
     3541 cggacgacac cgctcgagga aatggcactg gccgtggggc tctatgccct ggtccagctg
     3601 ccgctcagtg ccctgtggct cggccgattc cgttacggcc cgatcgaata cgtgtggcgc
     3661 cgcctcacgt acggcagcct gccgcgacgc gcattgcaac gcgcctgacg ccgggaggtc
     3721 gattcgcccg tgatcgggtc tcagtcgcga cgaaccagcc cgaactcttc ggcgagcagt
     3781 ccgtacgaac ggcgccggac cgccggatcg tgcgcccagg tgttgacgac gacctcgtcg
     3841 agcgagaagt cccgcgccag ctcgcgaatc ttcgcgccga cgttcgcccg cgaaccgacg
     3901 aaggagcggg cgcgcatcga cgccagctcg gcctcctcgg ccgaatcgaa accgctcgcg
     3961 gcgatctcgt cgggcgactg cagcgggccg aggcgtccgc gctgccggtc gatccgccac
     4021 cggtcgcgcg acagcgcgag gtgcgaagcc gcggcgtcgg tgtccgcgac gagcgcccag
     4081 atgcagaggg tcgactgcgg ttgcggatgc ctctcgctcg gccgatagcg cgcgcggtaa
     4141 agcgccatcg cttcgtcggc gcctcgcccg tcggtgaaga aatacgcgaa ggcatacggc
     4201 aggccgaagt gggcggcgag ctgcgcgccg tagtcggagc tgccgaggat ccagatctcg
     4261 ggcgcgtgcg gtccgagcgg atgcgcggtg atgccttcgt gggtgccgag cgacgtccag
     4321 gcatgcaggt cacgcacctg gatcggaaag tcttcggccg cgtggctggc gttcgggttg
     4381 agggcccgcg ccgtgcgcat gtcgccgccc ggcgcgcggc ccagtccgag gtcgatccgc
     4441 cccggcgcga gcgcctcgag aacgcgaaac tgttcggcca ccttgagggc gctgtagtgc
     4501 ggcagcatga tgcccgccga gccgatccgg atcgacgacg ttcgcgccgc gatcgccgcc
     4561 atgacgatct cgggcgccga gccgacgatc gtcggcaggc cgtgatgctc gctgacccag
     4621 aaacgcgagt agccgagctt ctcgcaatcc tcggccagcg agacggtgtc gcggatcgcc
     4681 gcgtcctcgc tgcttccggc gatggagacc gactggtcga gcaccgacag gcgcaagctc
     4741 atgcgacgct gcccgcgagc ccgctcgccg acgtggcgtg ccgtccgcgg ggatcgagcc
     4801 gaagatgaat gaaagacatg cggtcagcct cgaggatggg tcggatcgat ccagcgcacc
     4861 gtgtcgggct tctcgaccgg ctcgatgtcg aggttgaccg ccaccgcctc gccgtcgctg
     4921 cggcaaagga cgcattcgag cgtctcggtg gcgctggcgt tgatctcctg gtgcggaacg
     4981 aagggcggca cgtagatgaa atcgcccggc ccggcttcgg cggtgaactc gagcgcgttg
     5041 ccccagcgca tccgcgcccg ccccttgacg acgtagatca cgctttcgag atgaccgtgg
     5101 tgatgcgccc cggtcttggc atcggcgtgg atgtgcacgg tgccggccca gagcttctgc
     5161 gcgccgacgc gcgcgaaggt gatcgccgcc ttgcggttca ttcccggcgt ctgcgccgtg
     5221 ttcggatcga gctggccggc agcgatgacg cgaacgccgt cgtgcttcca cggcgcctcg
     5281 gcgaaagaag ggtgcgattc gtcgtgcgac atggttgctc ggcctcgtgt acagttgcgc
     5341 ccacagttat acgcaagaca gtggtttgtt cgtgattctc gttccaccct cgcggttctc
     5401 gcggcgggcg tttgaaatcc ccctgatcgg ttcttgagtg tcgctgcatg cgtcgcatgt
     5461 gcgccgcatt catttcccat tgattggata cacgttatga cgacccagac cggaaccgtg
     5521 aagtggttca acgaagccaa gggcttcgga ttcatcgccc aggacgccgg cggtgccgac
     5581 ctcttcgcgc acttccgcga catccagggc aacggcttca agatcctggc cgagaaccag
     5641 cgcgtcgaat tcgaagtcaa gcagggccag aagggcctgc aggcagcgaa cattcgcgtc
     5701 ctcgcctgaa tccgggcatt cgacgccgga cgatcgttcg atcgccggcg cggatgcagg
     5761 aaagccgcgg ctcgccgcgg ctttttttat gggcggctgc gcggcgacgt catgccctgc
     5821 gcgccgcgac gcctaagatc gccgctgcaa cgaggagacg aacatggctt caccgatcgg
     5881 cgagacgaat caccaggtcc ggctggcccg gcgaccggtc gggctgccga cgcgagccga
     5941 ctggagcttc accgccgaga ccctcaccga cgccgacgag ggcggcgtgc tggtcaagac
     6001 gctggccctg tcgctcgatc cggccatgcg cggctggatg aacgaaggca agagctacat
     6061 cgccccggtc ggcatcgacg aggtgatgcg ggccggcggc atcggcacga tcgtgtcgtc
     6121 gcgaagcccg aagttcgcgg tcggcgaccg cgtgagcggg cccctcgggg tgcaggaatt
     6181 cgcgcgtttc gatgcggccc agcttgcaag atcggcgctg cagcgcatcg acctgagcgc
     6241 cggatcgatg acgcaatggc tcaacgtcct cggcatgccg ggcatgacgg cgtacttcgg
     6301 actgctcgac gtcggccagc cgaaatcggg cgagaccgtg gtcgtctcgg gcgcggccgg
     6361 cgccgtcggc cagacggtcg gccagctggc gaagatcaag ggctgccgcg ctgtcggcat
     6421 cgccggcggc ccggcgaagt gcaaatgggt ggtcgagacg ctcggcttcg atgcctgcat
     6481 cgactacaag gccggcgccg tcaaggacgg cctgaaggcc cattgcccgg acggcgtcga
     6541 cgtttacttc gacaacgtcg gcggcgagat cctcgacacc gtgctgaccc gcctcgcccg
     6601 caaggccagg atcgtcatct gcggcgcgat cagccagtac aacaacaccg acgcggtcaa
     6661 gggacccgcc aactacctgt cgctgctcgt tcaccgcgcc cggatggagg gcatggtcgt
     6721 cttcgactac gccgaccgct tcccggtggc gatcgccgag ctggccggct acctgcgcga
     6781 cgggcgactg aaaagccgcg aagacgtcgt ctcgggcatc gaggccttcc ccgaagcgct
     6841 gctgcgcctg tttcgcggcg agaacttcgg caagctcgtg ctgaaggtcg cggaaggctg
     6901 atcccgccgc ggcggcgcgg cgtggcccgc ggctagactt cgccggttcg ggccgagccc
     6961 gagaatcgcc ccgatcgggc catgagcgcg ccgcccaatt cccgtctcac cgatgacctg
     7021 agcgacgacg gcccgccggc cgaggtgttg cgcgccttcg tcgcgcaatc gctcgagctg
     7081 ctggtggcga ccgatcggga cggcctgatc cgctggagca acgagcgctt ttcgagcgcg
     7141 accgggctgg tcgccgacgg ccgcgcaaac ctcctgcagg cgatcgccgg tggaagcgcc
     7201 ggcgagacga cccgtcgccg gctccaggcc ggcctcgacg acgacggtat cgacgccacc
     7261 ggtctgcgcc tggccggcag caatggcgcc gatctctggg tcgacgcgcg cgcacgccgc
     7321 gtcggcgacc aggtgctgtg gaccttcacc gacgtcacgt cgacgcgctt cctcgccaac
     7381 caggcgcgcc gccagggcga attgctcgac accgcccagg aattcggccg gcttggcgtg
     7441 tgggagcgca agatcccttc cggcgaaggc cgctgggatc gccatgtctt cgggttctgg
     7501 gggctcgacc cggccggcgg cacgccgagc ttcgtcgacg ccatcgaacg gatccatccg
     7561 gaagaccgcg agaagatgaa ttacctcgag tcgacccgtc gcgccgggcg ctactcgcaa
     7621 cgctatcgcg tcatccatcc cgacggccgc acccgctgga tccattccca gtgggaagtg
     7681 aagaacgggc cggcaggcgt tcccgaccgc gccttcggcg tgatgatgga cgacaccgag
     7741 gcgtacgagg cggcgcgcgc cctggtcgac gtcagcgcgc aactcaagat ggccgtcgag
     7801 ctcggcaaca tcgcgatctg gcggcacgac ctgcgcaccc agcgcatgta ctacagcaat
     7861 cgcgccttcg acctgctgca gatgccgacg cgctccgaag gcctgtcgat cgacgaggtg
     7921 cgcgccctca tccaccccga agacattccc ggcgtgatcg agtcggccga gcgcgcgctg
     7981 aagtccgacc gcccgaccga catggaggcg cgctaccggc gcaaggacgg cagctaccgc
     8041 tacgtcatga cccgccgagt cgtcgagcgc gacgccgcgg gccagccgat cgccttcgtc
     8101 ggcgtcgccc tcgacgtcac cgaggcggtg ctgcaccgcc gccacgccga cgcgctggcg
     8161 cgacgcatcg aagccgcctc gaaggcggcg ggcatcggcc tgtggacgac gtccgacaag
     8221 ccgcctcata ccgactggaa cacgcagacc ttcgcgatct tcgacgtcga tccgcccgtc
     8281 gtgcccgact tcagccactg gctgcgccgc tgcgtccatc cggacgatcg cacgcgcgtg
     8341 ggcaacgccg cgcacgccta cctcgcgcac ggcgacggcc ctttcgagat cgagttccgg
     8401 accgttcgcc gcgacggcgg cgttcgctgg gtcgtgatgc gtgccgatcg cgaccgcgag
     8461 tcgaccgacc agcgccgatt cctcggcgtc gcactcgacg tcaccgacca ccacgcggcc
     8521 gtcgacgcgt tgcgcgccgc cagcgaacgc gcgacgctca tcgcaagcta cgccggcatc
     8581 ggcatgtggg aaggaatgct cgacggctct ccggtgcgat gggacgcgca gatgttccat
     8641 ctgcgcggcc tgtcgcccga ggccgccgtg ccgtcgcggg acgagcggct ggcccgggtg
     8701 catcccgacg atcgtcaaca gcttctcgac tccctgcccg aagtcgagga gagcgcgcgg
     8761 gcgacctcct acgaattccg ctggaagatg cccgacggca gctggcgctg gctggcctcg
     8821 cgctcggcgc tggttcgtga cgcggccggg gtggcgctgc gacgcgtcg
//
LOCUS       DASRWS010000202         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1799709, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000202 DASRWS010000000
VERSION     DASRWS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799709"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VFF72_03715"
     CDS             complement(<1..1282)
                     /locus_tag="VFF72_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZV92297.1"
                     /translation="MSANDAGGRPAVKAPHRGLITASVMLASIMQALDNTIANVALPR
                     IQGSLSATQDQMTWVLTSYIIAAAIMTPLSGWLAGQVGRKRVFLFSIVGFTIASGLCG
                     LSGSLAEIVLARLAQGLCGAALIPMSQAVLLDINPKERHAKAMAIWIMAVTIGPILGP
                     ALGGWLTENYNWRWVFYINVPFGILAFLGILSFMPETEIRRSRFDFFGFAALSLAVGA
                     FQLMLDRGQLKDWFASTEILVEAIVAGLALYLFVVHMLTTKKSPFISPALFKDRNFLT
                     GNLFIFVVGVVLFATLALLPPLLQDLLGYPVVLTGLVTAPRGLGTLAAMLVVTRLMGK
                     VDTRWIIAAGFAMTAFSLWQMTHFYLQMGTASVVWSGLTQGFGTGFVYVPLATITFAT
                     LSPQFRNEGTAVFSLVRNIGSSVGISVVTTLLVRN"
     gene            complement(1279..2475)
                     /locus_tag="VFF72_03720"
     CDS             complement(1279..2475)
                     /locus_tag="VFF72_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZV92298.1"
                     /translation="MTTSTADEDDELASPLPPLPPVPAPPPRRSWKERLRLPLMVLGP
                     AVVAVVAAWFYLSGGRYESTDDAYVQAARVAISANVAGRVSELDVRENQVVHAGEVLF
                     RLDDAPFRIAVDEAKAQLAAARLQVDSLKANYRQRRAELASAEDTLAFQQKELDRQQR
                     LFASGIASQAQVDRAQHARDDARAQIAGAKQQISAVVASLGGNPDIALDRHPGVEQAQ
                     ARLERARLDLSYTTVKAPSDGVVTRVDNLQVGSYINAAAPLFALVSVGDIWVEANFKE
                     DQLTHMRVGQATTVKIDSYPGKTFQGDVASLSPGTGSQFSVLPPENATGNWVKVVQRL
                     PVRIRLRDLDPRYPLHAGLSADVSVDTRYRRHLFAADGDVAPDAAPGGAAAEARAVPA
                     AEVARR"
     gene            complement(2472..3011)
                     /locus_tag="VFF72_03725"
     CDS             complement(2472..3011)
                     /locus_tag="VFF72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZV92299.1"
                     /translation="MDRLKNVGFLLKEVSRLYVQRFEQHAAELSLTLAQCRALVRLEK
                     NEGISQARLAELTDVDPMSMVRILDRMQADGVVERRPDPGDRRARRLHLTPLARPMLD
                     EIWRLADRTRAEAFAGVGRAERESFIEVLERLDANLLALPVVPAPSPRAARRPSPVSR
                     TARGRARRSSSRPRAARKS"
     gene            complement(3322..>4609)
                     /locus_tag="VFF72_03730"
     CDS             complement(3322..>4609)
                     /locus_tag="VFF72_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZV92300.1"
                     /translation="EAPAPGMTAERRAAMGRAAVDAARAVGYVGAGTVEFIAHPDGRF
                     YFMEMNTRLQVEHPVTEMITGLDLVEWQLRIAAGEPLPKRQSELSIDGHAIEARVYAE
                     DPARGFLPSTGRLVHLAPPRESAHVRLDTGVEEGDSITPFYDPMIAKLIVHDRDRAAA
                     LARMREALASYRIVGVANNVEFLGRLVSTASFDTADLDTSLIEREHDALFAAPPAPSD
                     DVWLVAALAEVEHEARSAAEPPAGGSSRLEATSPWRRSDGWRLNGRAARRMVFRAQEA
                     AREVDAFAEGGGRWTLVLDGQGHAASAAAHGHGLVTATIGQRRFVTGVVAQGDRRHVF
                     LGGRSHVLEFVDPLAGPAAGHEEEGSGLLAPMPGKVIALVASPGAAVDKGAPLLMLEA
                     MKMEHTITAPHAGTVKAFRYAVGDQVDEGASLVDFE"
BASE COUNT          724 a         1579 c         1633 g          673 t
ORIGIN      
        1 tattgcgcac gagcagcgtc gtcaccaccg agatgccgac actgctgccg atgttgcgga
       61 ccaggctgaa caccgccgtg ccttcgttgc ggaactgagg cgacagcgtc gcgaaggtga
      121 tcgtggccag cggcacgtag acgaaaccgg ttccgaaccc ctgcgtgagg cccgaccaga
      181 ccaccgatgc ggttcccatc tgcagataga aatgcgtcat ctgccagagc gagaaggcgg
      241 tcatcgcgaa gccggcggcg atgatccagc gggtgtcgac cttgcccatc aggcgggtga
      301 cgaccagcat cgccgcgagc gtgcccaggc cgcgcggcgc cgtgaccagc ccggtcagga
      361 cgaccgggta gccgagcagg tcttgcagca gcggcggcag cagcgccagc gtcgcgaaca
      421 ggacgacgcc gacgacgaag atgaacaggt tgccggtgag gaagttgcgg tccttgaaga
      481 gggccgggct gatgaacggg ctcttcttcg tcgtcagcat gtggacgacg aagagataga
      541 gcgccaggcc ggcgacgatc gcttcgacca ggatttcggt cgacgcgaac cagtccttga
      601 gctggccgcg atcgagcatc agctggaagg caccgaccgc gaggctgagc gcggcgaatc
      661 cgaagaagtc gaagcgcgac ctgcggatct cggtctccgg catgaagctc aggatgccga
      721 ggaaggcgag gatgccgaac gggacgttga tgtagaagac ccagcgccag ttgtagttct
      781 cggtgagcca gccgccgagc gccggaccga ggatgggacc gatcgtcact gccatgatcc
      841 agatcgccat cgccttggcg tgccgctcct tcggattgat gtcgaggagc accgcctgcg
      901 acatcgggat cagcgccgct ccgcacagcc cctgcgcgag gcgcgccagg acgatctcgg
      961 ccaggcttcc ggacaggccg cacagccccg aggcgatcgt gaagccgacg atcgagaaga
     1021 gaaagacgcg cttcctgccg acctgcccgg cgagccagcc cgacagcggc gtcatgatgg
     1081 ccgcggcgat gatgtaggag gtcagcaccc acgtcatctg gtcctgcgtc gccgaaagcg
     1141 acccctggat ccgcggcagg gcgacgttgg cgatcgtgtt gtcgagcgcc tgcatgatcg
     1201 aggcgagcat caccgaggcg gtgatgaggc cgcggtgcgg cgccttgacc gccggccggc
     1261 cgccggcgtc gttcgcgctc accgccgtgc gacctccgca gccggcaccg cgcgggcttc
     1321 ggccgcggcg ccgccgggcg cggcgtcggg cgcgacgtcg ccgtcggcgg cgaagagatg
     1381 cctccggtag cgcgtgtcga cgctgacatc ggcgctgagc ccggcgtgca gcggatagcg
     1441 tgggtcgagg tcgcgaagac ggatgcgcac cggcaggcgt tgcaccacct tgacccagtt
     1501 gccggtggcg ttttccggcg gcagcaccga gaactgggaa cccgtgcccg ggctgaggct
     1561 cgcgacgtcg ccctggaagg tcttgcccgg atagctgtcg attttgaccg tcgtcgcctg
     1621 gccgacccgc atgtgcgtga gctggtcttc cttgaagttg gcctcgaccc agatgtcgcc
     1681 gacggagacg agcgcgaaga gcggcgccgc cgcgttgatg tagctgccga cctgcaggtt
     1741 gtcgacccgc gtcacgacgc cgtcgctcgg cgccttcacc gtcgtgtacg agagatcgag
     1801 gcgagcccgc tcgagccgcg cctgcgcctg ctcgacgccc ggatggcgat cgagcgcgat
     1861 gtccggattg ccgccgaggc tcgcgacgac ggcgctgatc tgctgtttcg ccccggcgat
     1921 ctgggcgcgg gcgtcgtcgc gggcgtgctg ggcccgatcg acctgcgcct gcgaggcgat
     1981 gcccgaggcg aagaggcgct gctggcgatc gagctccttt tgctggaaag ccagcgtgtc
     2041 ttcggcggac gcgagctcgg cgcgccgctg ccgatagttc gccttgagcg agtcgacctg
     2101 caggcgcgcc gccgcgagtt gcgccttcgc ttcgtcgacg gcgatgcgaa acggcgcgtc
     2161 gtcgaggcgg aacagcacct cgccggcgtg gacgacctgg ttctcgcgga cgtcgagctc
     2221 gctgacccgc ccggccacgt tggcgctgat ggcgacccgg gcggcctgca cgtacgcgtc
     2281 gtcggtcgac tcgtagcgac cgcccgaaag atagaaccac gcggccacga ccgcgacgac
     2341 ggccggcccc agcaccatca gcggcaggcg cagccgttcc ttccagctgc gtcgcggcgg
     2401 tggcgccggc accggcggca gcggtggcaa tggcgaggcg agctcgtcgt cttcgtcggc
     2461 ggtcgaggtg gtcatgattt cctggcagcc cgcggccgcg acgatgagcg acgcgcccga
     2521 cccctggccg tccgagagac cggcgacggc cggcgcgccg ctcgcggcga aggagccggc
     2581 acgacgggca gcgccagcag gttggcgtcg agccgctcca gcacctcgat gaacgactcg
     2641 cgctcggcgc ggccgacgcc cgcgaacgcc tcggcacggg ttcggtcggc gagccgccag
     2701 atctcgtcga gcatcggcct ggccaggggc gtgaggtgca ggcggcgggc gcgacgatca
     2761 cccggatcag ggcggcgctc gacgacgccg tcggcttgca tgcggtcgag gatgcggacc
     2821 atcgacatcg gatcgacgtc ggtgagctcg gccaggcgcg cctggctgat gccctcgttc
     2881 ttctcgaggc gaaccagcgc cctgcactgg gcgagcgtga gcgacagctc ggcggcgtgc
     2941 tgctcgaacc gctgcacata caggcggctc acttccttca gcaggaatcc gacgttcttg
     3001 aggcgatcca tgagacgcga gacgcagatg cggtcgaagc ggcccgctca ggccatatcg
     3061 ccactgatag tcacatttgt gatgatatct catgcgacaa gctggcgcat ggaaaacgag
     3121 cgggattcgc acgcgcgtcg tcctcaccgg ctgcgtgggt gcctgtccga agaaggggat
     3181 cacggtcgcg gcggcgacgg acggcggagc catccgcagg gtcgcgttcg gccgccacga
     3241 cgacgccggc gctgccgtcg acgtcctgtt cgacctcggg ccgcgaacgg atctcctggc
     3301 ctcaggcgac cctggccgcg ctcactcgaa gtcgaccagc gacgcgcctt cgtcgacctg
     3361 atcgccgacg gcgtagcgaa acgccttcac cgtcccggcg tgcggtgccg tgatcgtgtg
     3421 ctccatcttc atcgcctcga gcatcagcag cggtgcgccc ttgtcgaccg cggcacccgg
     3481 cgaggcgacg agcgcgatca ccttgcccgg catcggcgcc agcaggccgc tgccttcttc
     3541 ttcgtggcca gcagccggcc cggcgagcgg gtcgacgaac tcgaggacgt gcgatcggcc
     3601 accgagaaac acgtggcggc gatctccctg cgcgacgact ccggtgacga atcggcgctg
     3661 gccgatcgtc gccgtgacga gtccatgccc gtgcgccgcg gccgaggccg cgtgaccctg
     3721 cccgtcgagg acgagcgtcc agcgtccgcc gccttcggcg aaggcatcga cctcgcgcgc
     3781 cgcctcctgc gcgcgaaaca ccatccgccg cgccgcccgt ccgttgagac gccagccatc
     3841 cgatcgccgc cacggcgagg tcgcctccag ccggctcgac ccgccggctg gaggttctgc
     3901 cgccgaccgc gcttcgtgct cgacctcggc cagcgcggcg accagccaga cgtcgtcgga
     3961 cggcgcgggc ggcgcggcga agagcgcgtc gtgctcgcgt tcgatcagcg acgtgtcgag
     4021 gtcggccgta tcgaacgatg ccgtcgagac gagccggcca aggaactcga cgttgttggc
     4081 gacgccgacg atccggtagc tggcgagcgc ctcgcgcatc cgcgccagcg ccgcggcgcg
     4141 gtcgcggtcg tggacgatca acttggcgat catcgggtcg tagaacggcg tgatcgaatc
     4201 gccttcctcg acgccggtat cgagccgcac gtgcgccgat tcgcgcggcg gcgcgaggtg
     4261 cacgagccga ccggtcgaag gcagaaagcc gcgcgccgga tcctcggcat agacgcgcgc
     4321 ctcgatcgcg tgcccgtcga tcgacagctc ggactgtcgc ttcggcaggg gttcgccggc
     4381 ggcgatgcgc aattgccatt cgacgagatc gaggccggtg atcatctcgg tgaccgggtg
     4441 ctcgacctgc agccgcgtat tcatctccat gaaatagaag cggccgtcgg gatgcgcgat
     4501 gaactcgacg gtgccggcgc cgacgtagcc gaccgcgcgc gccgcgtcga ccgccgcgcg
     4561 acccatcgca gctcgccgct cggccgtcat tcccggcgcc ggcgcctct
//
LOCUS       DASRWS010000203         5877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2959011, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000203 DASRWS010000000
VERSION     DASRWS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959011"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFF72_03735"
     CDS             complement(<1..568)
                     /locus_tag="VFF72_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZV92301.1"
                     /translation="MGGSSAEREISLMSGTGVLAALRSSGVDAHAFDPAERDLAELRR
                     EGFARCFVALHGRHGEDGSVQGALELLGIPYTGSGVLASAIAMDKVMTKRVWIGEGLP
                     TPRFVRLAGDQQSREQTRAVPDVLGLPLIVKPPREGSSIGITKVRGYSDMPAAVELAA
                     GYDADVLCEEFIEGDEVTCPVLGEGLHAR"
     gene            complement(613..2022)
                     /gene="murC"
                     /locus_tag="VFF72_03740"
     CDS             complement(613..2022)
                     /gene="murC"
                     /locus_tag="VFF72_03740"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZV92302.1"
                     /translation="MKHAVKHIHFVGIGGAGMSGIAEVLHNLGYRVSGSDQSESATTR
                     ALAQRGIRVAIGHDAAHIAGAEAVVTSSAVKGDNPEVIAARSRRIPVVPRAVMLAELM
                     RLKQGIAIAGTHGKTTTTSLVTSVLAEAGVDPTFVIGGRLNAAGANSRLGGGDYIVVE
                     ADESDASFLNLLPVMAVVTNIDADHMDTYGHDFARLKQAFVEFIHRMPFYGAAILCAD
                     DPGVRSIMPMVSRPIVTYGFGAESMIRATEVVADAGTMRFKVQRRNGVRMPDLAVTLN
                     LPGAHNVLNALAAIAVAAELELADAPVVKGLAEFDGVGRRFQSWGELAARGGGGRFVL
                     IDDYGHHPVEMAATLAAARGAFPGRRIVLAFQPHRYTRTRDCFEDFVKVIGSADAVLL
                     GEVYAAGESPIVAADGRSLARALRVAGRVDPVFVDEIGAMASAVVEHARGGDVVIVMG
                     AGSIGAVPAQIVELAGGRP"
     gene            complement(2126..3205)
                     /gene="murG"
                     /locus_tag="VFF72_03745"
     CDS             complement(2126..3205)
                     /gene="murG"
                     /locus_tag="VFF72_03745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZV92303.1"
                     /translation="MTPRHLVVVAAGTGGHVIPGLAVAREMTRRGWTVSWLGTTTGME
                     NRLVPPAGYALDRLAFAGLRGKGWLGNVVGGLRLLGAIVASWRTLRRRAATAVLGMGG
                     YVCFPAGWAARGLGRPLVLMNADASLLLSNRALLPAARRIAFGFDGPAAATVGAKAVV
                     TGNPVRESIEAASPPASRLAGRTGALRVLVVGGSLGARVLNECLPESIALIDATARPL
                     VIHQTGEAAVESVRAAYAARAVEASVRAFIDDMPEQLATCDLIVCRAGAITVSELCAA
                     GVAAVLVPLVVSTTGHQRDNAEWMASHGAAIHLPQAELSPRRLAELIAGLTRDALLTM
                     ATKARALSRPHAAQRVADEIEAVAA"
     gene            complement(3202..4566)
                     /gene="ftsW"
                     /locus_tag="VFF72_03750"
     CDS             complement(3202..4566)
                     /gene="ftsW"
                     /locus_tag="VFF72_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZV92304.1"
                     /translation="MSAVLPESAASSKAMSARARVGAAIAGGWHALRARLGAPPPASP
                     AAPSAARPRARAAVARTIPVREWISVDRGQPARLAGFDRTLVLVIIGLLALGLVMVYS
                     ASVAMSDSPKFARYTSTYFLDRHLLFVTISVLVSLVVVQVPVSVWERLSPWIFVAALL
                     LLVLVLVPFIGRGVNGARRWIPLGFMSFQPSELCKLAMAMYAAGYMVRRMDVKENFFR
                     AVLPMALSLAVVGLLLLAEPDMGAFLVIATIAMGILFLGGVNGRMFLIVSTVLTGAFV
                     LMITTSSWRRERIFAYLNPWDDRYTLGKAYQLSHSLIAFGRGEIFGQGLGSSIEKLHY
                     LPEAHTDFLLAVICEELGFVGVAAVVFAFFWLARRLFHIGRQAIALDRVFAGLFAQGA
                     GIWVGGQAFINMGVNLGVLPTKGLTLPLMSYGGSAIVMNLVALAIVVRIDIENRQLMR
                     GGRA"
     gene            complement(4563..>5877)
                     /gene="murD"
                     /locus_tag="VFF72_03755"
     CDS             complement(4563..>5877)
                     /gene="murD"
                     /locus_tag="VFF72_03755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZV92305.1"
                     /translation="MKSPGLAPHDARIAALLAAAAEAKTPVAGELDLFMAALDDLHRR
                     LDYAPRIVAVTGTNGKTTTTALTALLVERSGRRVASAGNIGPTMLQTLADALDAAPGD
                     ALRESLPEVWVLELSSFQLADVARFDADAAAILNLTEDHLDWHGSMDAYAQAKAKVFG
                     TRAIVVVNRDDAAVMRLVPAPQVAVGTRKGRSPRVVSRHVVGFGAQPPDRPGDWGLVV
                     DNDMTWLARARDDAVGDEPALQYLMPADALRIRGRHNALNALAALALATAIGCPLAPM
                     LHGLREYRGEPHRVEFVATIDGVDAYDDSKGTNVGATVAALDGLGADRAPARLVVILG
                     GDGKGQDFTPLGPALARHARGVVTIGRDAGSIETVVESAGVRAQRCATLPAAVEAAFA
                     LAVPGDAVLLSPACASLDMFRNYGHRAEVFVAAVRELAAGRGIVS"
BASE COUNT          917 a         2085 c         2122 g          753 t
ORIGIN      
        1 cgcgcgcatg caggccctcg ccgagcaccg ggcaggtgac ctcgtcgcct tcgatgaatt
       61 cctcgcacag cacgtcggcg tcgtaacccg cggcgagctc gacggcagcc ggcatgtcgg
      121 agtagccgcg caccttggtg atgccgatcg aggatccttc gcgtggcggc ttgacgatga
      181 gcggcaggcc gagcacgtcg ggcacggcgc gcgtctgttc gcgcgactgc tggtcgccgg
      241 cgaggcgaac gaagcgcggc gtcggcaggc cttcgccgat ccagacgcgc ttggtcatga
      301 ccttgtccat cgcgatcgcc gaggcgagga cgcccgatcc cgtgtacggg atgccgagca
      361 gctcgagggc gccctgcacg ctgccgtcct cgccatgccg gccgtgcagg gcgacgaagc
      421 agcgcgcgaa gccttcgcgg cgcagctcgg cgagatcgcg ttcggccgga tcgaaggcat
      481 gcgcgtcgac tccgctcgac ctcagtgcgg cgagaacgcc ggtccccgac atcagggaga
      541 tctcgcgctc ggcggagctg ccgcccatca gcacggcaac ccgacccagc gcggcgacgt
      601 cgaccgtcgt gctcacggcc gccctcctgc cagctcgacg atctgtgccg gcacggcgcc
      661 gatcgagccg gcgcccatga cgatgacgac gtcgccgccg cgcgcgtgct cgaccacggc
      721 cgaggccatc gcgccgatct cgtcgacgaa gacgggatcg accctgccgg cgactcgcaa
      781 tgcgcgggcc agcgaacggc cgtcggcggc gacgatgggc gattcgccgg cggcatacac
      841 ctcgcccagc agcaccgcat cggcgctgcc gatcaccttg acgaaatcct cgaagcagtc
      901 gcgcgtgcgc gtgtatcggt gcggctggaa ggcgaggacg atgcggcgtc ccgggaaggc
      961 accgcgcgcc gccgccagcg tcgccgccat ctcgaccggg tgatggccgt agtcgtcgat
     1021 caggacgaag cggccgccgc cgccgcgcgc cgcgagctcg ccccagctct ggaagcgacg
     1081 gccgacaccg tcgaattcgg ccaggccctt gacgaccggc gcatcggcca gctcgagctc
     1141 ggcggcgacg gcgatcgccg cgagcgcatt gaggacgttg tgggcgcccg gcaggttgag
     1201 cgtcaccgcg aggtcgggca tgcgcacgcc gttgcgccgc tgcaccttga accgcatcgt
     1261 cccggcgtcg gcgaccacct cggtggcgcg gatcatcgac tcggcgccga agccgtaggt
     1321 gacgatcggc cgcgagacca tcggcatgat cgagcgcacg cccggatcgt cggcgcacag
     1381 gatcgccgca ccgtagaacg gcatcctgtg gatgaactcg acgaacgcct gcttcagcct
     1441 ggcgaagtcg tggccgtagg tgtccatgtg gtcggcgtcg atgttggtga cgaccgccat
     1501 cacgggcagc aggttgagga aggaggcgtc ggactcgtcg gcctcgacga cgatgtagtc
     1561 gccgccgccg agccgcgagt tcgccccggc ggcgttgaga cggccgccga tgacgaacgt
     1621 cggatcgaca ccggcctcgg cgaggacgct cgtgacgagc gaggtcgtcg tcgtcttgcc
     1681 gtgcgttccg gcgatggcga tgccctgctt gaggcgcatc agctcggcca gcatgacggc
     1741 gcgcggcacc accgggatcc gccggctgcg ggcggcgatg acttcggggt tgtcgccctt
     1801 cacggcgctc gacgtgacga ccgcttcggc gccggcgatg tgcgcggcat cgtggccgat
     1861 ggcgacgcgg attccgcgct gcgccagcgc ccgcgtcgtc gcgctctcgc tctggtcgga
     1921 accggagacg cgatagccga ggttgtgcag cacctcggca atgccgctca tgccggcccc
     1981 gccgatgccg acgaagtgga tgtgcttgac cgcgtgcttc atcgatcgcg cccttcgccg
     2041 gcaccggcgg caggcgccga cacaggaaag gccacccgct gcgcggctgg ccggccgacg
     2101 cagtggatgt gcttgaccgc gagcttcatg ccgccaccgc ctcgatctcg tcagccactc
     2161 gctgcgcggc atgcggcctg gacagggcgc gggccttggt cgccatcgtc aacagcgcgt
     2221 cacgggtcag gccggcgatc agctcggcga ggcggcgagg cgacagctcg gcttgcggca
     2281 ggtggatcgc cgcgccgtgc gacgccatcc attcggcgtt gtcgcgttga tggccggtgg
     2341 tgctgacgac gaggggcacc agcaccgcgg cgacgccggc ggcgcagagc tcgctgacgg
     2401 tgatggcgcc ggcgcgacag acgatcaggt cgcacgtcgc cagctgctcg ggcatgtcgt
     2461 cgatgaacgc gcgcacgctc gcctcgaccg cgcgggcggc gtaggcggcc cgcaccgact
     2521 cgacggcggc ctcgccggtc tggtgaatga cgagcgggcg tgccgtcgcg tcgatgagcg
     2581 cgatcgactc gggaaggcac tcgttgagca cgcgcgcgcc gaggctgccg cccacgacca
     2641 gcacgcgcag cgcgccggtg cgtccggcca ggcgcgaggc cggcggcgac gccgcctcga
     2701 tcgactcgcg caccgggttg ccggtcacga ccgccttggc gccgaccgtc gccgccgcgg
     2761 gcccgtcgaa gccgaaggcg atccgacgcg ccgccggcag caaggcccgg ttgctgagca
     2821 gcaggctcgc gtcggcgttc atcagcacca gcggacgacc gagtccgcgc gccgcccagc
     2881 cggcgggaaa gcagacgtag ccacccatgc cgagcaccgc cgtcgccgcg cggcggcgca
     2941 gcgtccgcca tgacgcgacg atcgcgccca gcaggcgcag accgccgacg acgttgccga
     3001 gccagccctt gccgcgcagg ccggcgaagg ccagccgatc gagcgcgtag ccggccggtg
     3061 gcacgagacg attctccatg ccggtggtgg tgcccagcca gctcaccgtc cagccccggc
     3121 gcgtcatttc gcgagcgacg gccagacccg gaatcacgtg accccccgtg ccggcggcga
     3181 cgacgacgag atggcgcggc gtcatgcgcg gcccccgcgc atgagctgtc gattctcgat
     3241 gtcgatgcgc acgacgatgg ccagggcgac gaggttcatg acgatcgccg agccgccgta
     3301 gctcatcagc ggcagcgtca atcccttggt cggcagcacg ccgaggttga cgcccatgtt
     3361 gatgaacgcc tggccgccga cccagatgcc ggcgccctgc gcgaagaggc cggcgaagac
     3421 gcggtcgagg gcgatcgcct ggcggccgat gtgaaagagc cggcgggcca gccagaagaa
     3481 cgcgaagacg accgcggcga cgccgacgaa gccgagctcc tcgcagatga cggccaggag
     3541 gaagtcggta tgcgcctcgg gcaggtaatg cagcttctcg atgctcgagc ccaggccctg
     3601 gccgaagatc tcgccgcggc cgaaggcgat cagcgagtgc gagagctggt aggccttgcc
     3661 gagggtgtag cggtcgtccc agggattgag gtaggcgaag atgcgctcgc gccgccacga
     3721 cgaggtcgtg atcatcagca cgaaggcgcc ggtgagcacc gtgctgacga tcaggaacat
     3781 ccggccgttg acgccgccga gaaagaggat gcccatcgcg atcgtggcga tgacgaggaa
     3841 ggcgcccatg tcgggctcgg caagcagcag caggccgacc accgccagcg agagcgccat
     3901 cggcagcacg gcgcgaaaga agttctcctt cacgtccatc cgccgcacca tgtatccggc
     3961 cgcatacatc gccatcgcca gcttgcacag ctccgacggc tggaagctca tgaagccgag
     4021 cgggatccag cgccgcgctc cgttgacgcc cctgccgatg aacggcacca gcaccagcac
     4081 gagaagaagc agcgcggcga cgaagatcca gggcgagagc cgctcccaga ccgacaccgg
     4141 cacctggacg acgaccagcg agacgagcac cgagatggtg acgaagagca ggtgcctgtc
     4201 caggaagtag gtggacgtgt agcgggcgaa cttggggctg tccgacatcg ccaccgaggc
     4261 gctgtagacc atcaccaggc ccaacgccag caggccgatg atgaccagca ccagggtgcg
     4321 atcgaatccc gcgaggcgag ccggctggcc gcggtcgacg ctgatccact cgcgcaccgg
     4381 gatggtccgg gccaccgcgg cgcgggcccg cgggcgcgcc gccgaaggcg cggctggcga
     4441 cgcaggcggc ggcgcgccga ggcgggcgcg caaggcatgc cagccgccgg cgatggcagc
     4501 gccgaccctg gcgcgagcgg acatcgcctt cgacgaggcg gccgactccg gcagcaccgc
     4561 gctcacgaga cgatccccct gccggccgcg agctcgcgaa ccgccgcgac gaagacttcg
     4621 gcgcgatgcc cgtagttgcg aaacatgtcg aggctggcgc aggccgggct gagcagcacg
     4681 gcgtctcccg ggaccgcaag tgcaaaggcc gcctcgaccg ccgccggcag cgtcgcgcag
     4741 cgctgcgccc ggacgcccgc cgactcgacc accgtctcga tcgatccggc gtcgcggccg
     4801 atcgtcacga cgccgcgcgc gtgccgcgcc agcgccggac ccagcggcgt gaaatcctgg
     4861 cccttgccgt cgccgccgag gatgacgacg agcctggccg gcgcccggtc ggcgccgagg
     4921 ccgtcgaggg ccgccaccgt cgcgccgaca ttggtgccct tgctgtcgtc gtaggcatcg
     4981 acgccgtcga tcgtcgccac gaattcgacg cgatgcggct cgccgcgata ctcgcgcaag
     5041 ccatgcagca tcggcgcgag cgggcaaccg atcgccgtcg ccagcgccag cgcggccagc
     5101 gcgttcagcg cgttgtgccg gccgcggatg cgcagcgcgt cggcgggcat caggtattgc
     5161 agcgccggtt cgtcgccgac ggcgtcgtcg cgggcacgag ccagccacgt catgtcgttg
     5221 tcgacgacga gcccccagtc gcccggtcga tcgggcggct gcgcgccgaa gccgacgacg
     5281 tgccgggaaa cgacgcgcgg gctgcgtccc ttgcgggtac cgacggccac ttgcggcgcc
     5341 ggcaccaggc gcatgaccgc cgcatcgtcg cggttgacga cgacgatcgc gcgggtgccg
     5401 aagacctttg ccttggcctg cgcgtaggcg tccatcgagc cgtgccagtc gaggtggtcc
     5461 tcggtcaggt tgaggatcgc cgccgcatcg gcgtcgaagc gggcgacgtc ggcgagctgg
     5521 aagctcgaca gctcgagcac ccagacctcg ggcagcgact cgcgcagcgc atcgcccggc
     5581 gccgcgtcga gcgcatcggc gagcgtctgc agcatcgtcg ggccgatgtt gcctgccgac
     5641 gcgacgcgcc ggccgctgcg ctcgaccagc agcgccgtca gcgccgtcgt cgtcgtcttg
     5701 ccgttggtgc cggtgacggc gacgatccgc ggcgcgtagt cgagacgtcg gtgcaggtcg
     5761 tcgagcgccg ccatgaagag atcgagctcg cctgcgacgg gtgtcttcgc ctcggccgcg
     5821 gcggcgagaa gcgcggcgat gcgcgcgtcg tgcggcgcca gaccggggct tttcatg
//
LOCUS       DASRWS010000204         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_944257, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000204 DASRWS010000000
VERSION     DASRWS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944257"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..880
                     /locus_tag="VFF72_03760"
     CDS             215..880
                     /locus_tag="VFF72_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZV92306.1"
                     /translation="MYLPTHFAETRPEVLHQLVREHPFGLLVTQGGGRGIEANGVPFV
                     LDADPEGGPGILRAHVARANTIWREARSDVDSLVVFQGAQVYVSPAWYPSKAEHGKVV
                     PTWNYIMVQGRGRLRAIDDAEWLRRFVSSLTDDHEKRRVAQSPAQAATWAVTDAPAEF
                     VETMLKAIVGIEIVLTSLVGKWKASQNRPAADREGVARGLDDLAGSDAAAMARQVLAD
                     RDG"
     gene            916..1728
                     /locus_tag="VFF72_03765"
     CDS             916..1728
                     /locus_tag="VFF72_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZV92307.1"
                     /translation="MQATTTRWRLLRMLAVLLSAGLGTSMAAAFEDTMAQRVLACTGC
                     HGAQGRAAPDGYYPRLAGKPAGYLYNQLLNFRDERRHYALMNNLLAPLDDAYLREIAA
                     HFAGLEVPYPAPAPIVLDAQSRAAAERIVREGDARRGVPACVACHGKAMTGTAPFVPG
                     LLGLPRDYLNAQLGAWREDRRHAQAPDCMSRVAKSIDAREIGAIAGWLASQPVPADSK
                     PVGAFAGPLPFACGGVTNAPVAVPATGHAVSVEPPAAPAATAETARQHGTRR"
     gene            1725..>2057
                     /locus_tag="VFF72_03770"
     CDS             1725..>2057
                     /locus_tag="VFF72_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZV92308.1"
                     /translation="MTRRRRGIRVLVASVSAVIALLGLFAAIVAVLNVRGEAPVRPRE
                     AGSVGPIDPALIVRGEYLSRIGDCAACHTKRGGPFFAGGRAIETPFGSVYSSNLTPDV
                     QTGLGSWSA"
BASE COUNT          315 a          733 c          708 g          301 t
ORIGIN      
        1 aaggccgcat caggaatcga tcggcgtcgc gacgaacggg aaacgccgtc gaagcagtct
       61 agcttcggcc ggtcggcgag gcgatcggcc cgcgggagcg cggcaacgca cgagccggca
      121 tcaccggaac cggcctgcga ctgaggcaag atcgcggaat cgcgatctcg cgtgtgatcg
      181 atttgccgct ttgcccgaac caaggtcatc gtccatgtac cttcccacgc atttcgccga
      241 gacccggccc gaggtcctgc accagctcgt tcgcgagcat cccttcggac tcctggtgac
      301 gcaaggcggc ggccgcggca tcgaagccaa cggcgtcccg ttcgtgctcg acgccgaccc
      361 cgagggcggt cccggcatcc tgcgcgcgca cgtcgcccgg gccaacacga tctggcgtga
      421 agcgcgcagc gatgtcgatt cgctcgtcgt cttccagggc gcccaggtct acgtgtcgcc
      481 ggcgtggtat ccgagcaagg ccgagcacgg caaggtcgtg ccgacgtgga actacatcat
      541 ggtgcaaggg cgaggacgcc tgcgcgcgat cgacgacgcc gagtggttgc gccgcttcgt
      601 gtctagcctc accgacgatc atgagaaacg gcgcgtcgcc cagtcgccgg cccaggccgc
      661 gacctgggcg gtgaccgacg cgcccgccga attcgtcgag acgatgctga aggccatcgt
      721 cggcatcgag atcgtcctga cgtcgctggt cggcaagtgg aaggcgagcc agaaccggcc
      781 cgccgccgat cgcgaagggg tggcgcgcgg cctggacgat ctcgccggaa gcgatgcggc
      841 ggcgatggcg cgtcaggtgc ttgcagatcg ggacggatga gcggcggcca gagggcagac
      901 gtcgcggagg gcgcgttgca agccacgaca acgcggtgga ggctgctgcg catgctcgcc
      961 gtgctgctgt cggcgggcct cggcacgtcg atggcggcgg ccttcgagga cacgatggcg
     1021 caacgcgtcc tcgcctgcac cggctgccat ggcgcgcagg gccgcgccgc gcccgatggc
     1081 tactacccgc gtctcgccgg aaagccggct ggctacctct acaaccagct cctcaacttt
     1141 cgcgacgagc gccgtcacta cgcgctgatg aacaacctgc tggcgccgct cgacgatgcc
     1201 tacctgcgcg agatcgccgc tcacttcgcc gggctcgaag tcccttatcc ggcgccggcg
     1261 ccgatcgtcc tcgacgcgca gtcgcgcgca gcggccgagc gcatcgtgcg cgagggcgac
     1321 gcgaggcgcg gcgtgccggc ttgcgtcgcc tgccacggca aggcgatgac cggcacggcg
     1381 ccgttcgttc ccggcctgct cggcctgccg cgcgactacc tgaacgccca actgggcgcg
     1441 tggcgggagg accggcgcca tgcccaggca cccgactgca tgagccgcgt cgccaagtcg
     1501 atcgatgcgc gggaaatcgg cgccatcgcc ggctggctcg cgtcgcaacc cgttcccgcc
     1561 gattccaagc ccgtcggggc attcgcggga ccactgcctt tcgcttgcgg tggcgtcacg
     1621 aacgcgccgg tggccgtgcc tgcaaccgga cacgccgtct ccgtcgaacc cccggcggca
     1681 cctgccgcga cagccgaaac ggcacggcag cacggcaccc gccgatgaca cgacgccgac
     1741 gcggcatccg agtcctcgtc gccagcgttt cggcggtgat cgcgctgctg ggcctgttcg
     1801 ccgccatcgt tgccgtcctc aatgtccgcg gtgaagcacc ggtccggcca cgcgaggccg
     1861 gcagtgtggg gccgatcgat ccggcattga tcgtgcgcgg cgagtacctg tcgcggatcg
     1921 gcgactgcgc cgcttgccac acgaaacgcg gtggcccgtt cttcgcaggc ggccgcgcca
     1981 tcgagacgcc tttcggcagt gtctacagct cgaacctgac ccccgatgtg cagaccggtc
     2041 tcggcagctg gtcggcc
//
LOCUS       DASRWS010000205         9478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3822512, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000205 DASRWS010000000
VERSION     DASRWS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3822512"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFF72_03775"
     CDS             complement(<1..448)
                     /locus_tag="VFF72_03775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZV92309.1"
                     /translation="MTDRLFIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIE
                     AGFAASSNGDFEAVRAIAQAIRGSTVCSLARANDRDISRAAEALEGGESTRIHIFLAT
                     SDLHMEKKLRMTREQVFEQARLSVRFARKHCADVEFSPEDGSRSEPD"
     gene            complement(608..1441)
                     /gene="pssA"
                     /locus_tag="VFF72_03780"
     CDS             complement(608..1441)
                     /gene="pssA"
                     /locus_tag="VFF72_03780"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZV92310.1"
                     /translation="MHDDVLVPEATPSPPPPPPRLRRKGVYILPNLFTLGALFGGFYA
                     VVMSMNGRFEQAATGVFWAMILDSLDGRIARLTKTQSVFGEHMDSLSDMVSFGAAPAL
                     IGYEWSLRGLGKWGWVAAFVYCSCAALRLARFNTNVGVVDKRFFQGLPSPAAAALVMG
                     FIWLMDNQGYSGIEEGPWLSWTTFAITLYAGLTMVTNAPFYSFKDLSLKRSVPFVVIV
                     LIAIGIAVIALHPPFVLAAMVLAYGVSGYVVYAWKKFKGKPVSVIATSTEEPDEQGLH
                     H"
     gene            complement(1500..2516)
                     /gene="ilvC"
                     /locus_tag="VFF72_03785"
     CDS             complement(1500..2516)
                     /gene="ilvC"
                     /locus_tag="VFF72_03785"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZV92311.1"
                     /translation="MKVYYDKDADLSLVKGKSVTIIGYGSQGHAHAQNLSDSGVRVTV
                     GLRKGGASWAKAEAAKLKVAEVGEAVKSADIVMMLLPDEQIAEVYRKEVEPNIRQGAS
                     LAFAHGFNVHYGQVVPRADLDVWMVAPKAPGHTVRSTYQQGGGVPHLIAVHADKSGKA
                     RDLALSYAVANGGGKAGVIETNFREETETDLFGEQAVLCGGTVELIKAGFDTLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVSGPKVVDGRTRAAMKQ
                     MLDDIQSGEYAKSFILENRAGAPTLMSKRRLTAEHPIEVVGEKLRAMMPWIRKNRLVD
                     QTRN"
     gene            complement(2539..3030)
                     /gene="ilvN"
                     /locus_tag="VFF72_03790"
     CDS             complement(2539..3030)
                     /gene="ilvN"
                     /locus_tag="VFF72_03790"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZV92312.1"
                     /translation="MKHIIAVLLENEPGALSRVVALFSARGYNIETLTVAPTEDASLS
                     RMTIVTTGSDEVIEQITKHLNRLIEVVKVVDLTEGAYTERELMLIKVRAVGKERDEMK
                     RMAEIFRGRIIDVTEKSYTVELTGAAEKLDAFLEAIDRNVILETVRTGSSGIGRGERI
                     LRI"
     gene            complement(3121..4896)
                     /locus_tag="VFF72_03795"
     CDS             complement(3121..4896)
                     /locus_tag="VFF72_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HZV92313.1"
                     /translation="METSALELKRAPAPSLRPAPAGDPNGSEILVRCLQAENVRYLWG
                     YPGGAVLYIYDALYKQQTIEHVLVRHEQAAVHAADGFARATGEVGVALVTSGPGVTNA
                     ITGIATAYMDSIPMVIITGQVPTPAIGLDAFQECDTVGITRPVVKHNFLVKDVRDLAT
                     TMKKAFHIARTGRPGPVVVDVPKDVSMATTPYHYPATIEMRSYNPVKKGHGGQIRKAA
                     QLLLGAKRPYVYAGGGVVIGNASAELRELVDLLGFPCTNTLMGLGAYPASDPKFLGML
                     GMHGTYEANMTMQNCDVLLAIGARFDDRVIGNPKHFASVERKIVHIDIDPSSISKRVR
                     VDVPIVGDVRDVLQELTALLRESASRPDLKALSPWWAQINEWRKRDCLAYAGDGDVIK
                     PQHVVDTLWQLTKDRDTYITSDVGQHQMWAAQYYRFEQPRRWINSGGLGTMGVGLPYA
                     MGIKLARPDADVYCITGEGSIQMCIQELSTCLQYKTPVKIVSLNNRYLGMVRQWQEID
                     YGGRYSHSYMDALPDFVKLAEAYGHVGLRIDKPADVEPALKEAIRLKERTVFLDVRTD
                     PAANVWPMVQAGKGISEMLLGSEDL"
     gene            5147..5713
                     /locus_tag="VFF72_03800"
     CDS             5147..5713
                     /locus_tag="VFF72_03800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZV92314.1"
                     /translation="MATDKELADFLKSVEKRAWKRAVYAVRDEDAALDIVQDAMIRLS
                     EKYVDRPVAELPLLFQRIVSNATMDWFRRQKVRNQVVRNFSEFESAGNDGDFDLLETL
                     QALGSSLESESAADSVSRAQILQVIDHEVAQLPARQREAFLLRYWEELDVAETAASMG
                     CSEGSVKTHCSRAVHTLAKALQAKGISP"
     gene            5710..6150
                     /locus_tag="VFF72_03805"
     CDS             5710..6150
                     /locus_tag="VFF72_03805"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HZV92315.1"
                     /translation="MNTLESTYRTAAREAIEARFARGVVARLSEQADHVSPEVGERLR
                     FARQKALEAAQRARASGAVEAAGRSGRSATAGFARASWWLRVASVLPLVALVGGLVLI
                     QESQTRSQITTAAQVDTELLGDDLPLNAYRDAGFVEFLKTPDSE"
     gene            6162..7064
                     /locus_tag="VFF72_03810"
     CDS             6162..7064
                     /locus_tag="VFF72_03810"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZV92316.1"
                     /translation="MRARSARTARIAAASVLLVGACLVVASRGMAQPSPSAPAGRPVA
                     VPPHPAAVRATGGPVRPRPEEGVRWQKLNAAQRQALAPLAQQWSSIDAPRKQKWLVIA
                     QRFNSLPPLEQARITDRMTAWAKLTPAERGEMRLRYQESRQVRGDRSARWQAYQQLSP
                     EQRKEFEARAVLPAVAPTALQKRDTAGRQGSPGKINIVSNPALAQPPRPVAPTVVQAS
                     RGATTHLITRPVAPPAHQQTGMPKIEATPEFVNRSTLLPRRGPQAAGVMPPYIPIRPM
                     AAPRVQPAATAAHPGKTPEPAPAR"
     gene            7061..7624
                     /locus_tag="VFF72_03815"
     CDS             7061..7624
                     /locus_tag="VFF72_03815"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZV92317.1"
                     /translation="MSEAPAAAAMPGAAPTTPSIARRMACFVYEATLLFGLGLIPGVI
                     GAVFVAQTGQHGVLQSPTALRIYAWILYGVYFVWFWTVRGQTLAMQTWRIGLVTAAGH
                     LPTQARALTRYVACCIAWFGPSTAIAFALRLPPSTSLALAAAWIVVYALLARAAPGRQ
                     FWHDIACGTRLVDTRAIDWASLRSRPR"
     gene            complement(7605..7943)
                     /locus_tag="VFF72_03820"
     CDS             complement(7605..7943)
                     /locus_tag="VFF72_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZV92318.1"
                     /translation="MKQITAIVKPFKLEEVREALADIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVKDADAERCIEAVVKAAKTGKIGDGKIFVTAIESVVRI
                     RTGELNEDAI"
     gene            8007..9215
                     /locus_tag="VFF72_03825"
     CDS             8007..9215
                     /locus_tag="VFF72_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV92319.1"
                     /translation="MPASKMPARNAPRAADADAWNVRVCVDPGEIDAAAWDALVDAQP
                     SATPFMTLAYLRALHASGSASAETGWSPHFLLLENAGELLAACPLYLKDHSYGEYVFD
                     WAWADAYRRHGLDYYPKLLDAVPFTPVPGPRLLARDPEHRLVLLRGIEQLARSAGLSS
                     AHLLFLDADDQAAARAAGWSMRTTVQFHWHNREPTPWLDFADFLASLQREKRKKIQQE
                     RRRVAEAGVTFTASVGRAISDTDWDFFYRCYTLTYRAHHSTPYLTRDFFARAASDLAD
                     HWLLFVAYRQGRPIASSLVVIDPQRKAAFGRYWGAVEHVSCLHFDACYYEPLAWCIAH
                     GYRRFEGGAQGEHKMARGLLPVQTFSAHWLAHPQFARAIDDFLAREGAGIEEYVGELN
                     ERLPFKPPPG"
     gene            complement(9212..>9478)
                     /locus_tag="VFF72_03830"
     CDS             complement(9212..>9478)
                     /locus_tag="VFF72_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZV92320.1"
                     /translation="AGGDAKLLAVPIDKILPIYRHWQKPADMNELRLKQIQHFFEHYK
                     DLEAGKWVKTMGWDGPDAARDEIRQGIASYARQHPRTSAGAATL"
BASE COUNT         1474 a         3224 c         3147 g         1633 t
ORIGIN      
        1 aatccggttc cgaccggctg ccgtcctcgg gcgagaattc gacgtcggcg cagtgcttgc
       61 gcgcgaagcg caccgagagc cgcgcctgtt cgaacacctg ctcgcgcgtc atgcgcagct
      121 tcttctccat gtgcaggtcg ctggtcgcga ggaagatgtg gatgcgggtc gactctccgc
      181 cctcgagcgc ctcggccgcg cgcgagatgt cgcgatcgtt ggcacgggcg agcgagcaca
      241 ccgtcgagcc gcgaatcgcc tgggcaatcg cgcgcacggc ctcgaaatcg ccgttcgacg
      301 acgcggcgaa accggcctcg atgacatcga ccttgagccg ctcgagctgg cgcgcgatac
      361 gcagcttctc gtccttggtc atcgaggcgc cgggcgactg ctcgccgtcg cgcagggtgg
      421 tgtcgaagat gaacaggcga tcggtcaagg gagcctccgg aaaacaaaaa ggcccgctgc
      481 gtgatgcgag cgggccggtt tgacgaaacg ttgtgacgag gtcgttcagc gagccctgtg
      541 cggtcgaaga agaaggagcc gagccgagga aattcgctgc atcacgatct caatatagca
      601 cgccaggtca gtggtgcaag ccttgttcgt cgggttcctc ggtcgacgtt gcaatcacgc
      661 tgacaggctt tcccttgaac ttcttccagg cgtagacgac gtagcccgag acgccgtagg
      721 ccaggaccat cgcagcgagg acgaacggtg ggtgcagtgc gatgacggcg atgccgatgg
      781 cgatcaggac gatgacgacg aagggcaccg agcgcttcag gctcaggtcc ttgaagctgt
      841 agaaaggcgc gttggtcacc atcgtgagcc cggcatagag cgtgatggca aaggtcgtcc
      901 agctgagcca ggggccctcc tcgatgccgg agtatccctg gttgtccatc agccagatga
      961 agcccatgac cagcgccgcc gctgccgggc tcggcaggcc ctggaagaag cgcttgtcga
     1021 cgacgcccac gttggtattg aagcgggcca gccgcaatgc cgcgcacgag cagtacacga
     1081 acgcggcgac ccagccccac ttgccgagcc cgcgaagcga ccattcatag ccgatgagcg
     1141 ccggcgccgc accgaacgac accatgtcgg agagcgaatc catgtgttcg ccgaagacgc
     1201 tctgcgtctt cgtgagccgg gcgatgcgtc cgtcgaggct gtcgaggatc atcgcccaga
     1261 acacgcccgt ggcggcttgc tcgaagcggc cgttcatcga catcacgacg gcgtagaagc
     1321 cgccgaagag cgcgcccagg gtaaacaggt tgggcaggat gtagacgccc ttgcgccgca
     1381 accgcggcgg cggcggcggc ggagacggag tcgcctcggg gacgagtacg tcgtcgtgca
     1441 tcgtcatcgt gcgattagcg gctgcccgct cgtctgcccg ccccggcccg gcgacccgct
     1501 caattgcgcg tctggtcgac gaggcggttc ttcctgatcc acggcatcat cgcccgcagc
     1561 ttctcgccga ccacctcgat cggatgctct gccgtcaggc gccgcttgct catcagcgtc
     1621 ggcgccccgg cccggttctc gaggatgaag ctcttcgcgt attcgccgct ctggatgtcg
     1681 tcgagcattt gcttcatcgc cgcgcgcgtg cggccgtcga cgaccttcgg accgctcacg
     1741 tattcgccgt actccgcgtt gttggagatc gagtagttca tgttggcgat gccgccctcg
     1801 tagatgaggt cgacgatgag cttgagctcg tgcaggcatt cgaaatacgc catttcgggc
     1861 gcgtagccgg cctcgacgag cgtgtcgaac ccggccttga tcagctcgac cgtgccgccg
     1921 cacaacaccg cctgttcgcc gaacaggtcg gtctcggttt cctcgcggaa gttcgtctcg
     1981 atcacccccg ccttgccgcc gccgttggcg accgcgtagc tgagggccag gtcgcgggcc
     2041 ttgccggact tgtcggcgtg caccgcgatc aagtgcggca cgccgccacc ttgctggtac
     2101 gtggagcgaa cggtgtggcc gggcgccttc ggcgcgacca tccagacgtc gagatcggcg
     2161 cgaggcacga cctggccgta gtggacgttg aagccgtgcg cgaacgcgag cgatgcaccc
     2221 tgccggatgt tcggctcgac ttccttgcga tagacctcgg cgatctgctc gtcgggcaga
     2281 agcatcatga cgatgtcggc cgatttgacc gcctcgccca cttcggcgac cttgagcttg
     2341 gcggcttccg ccttggccca cgacgccccg cccttgcgca ggccgaccgt gacgcgcacg
     2401 ccgctgtcgg acaggttctg cgcatgcgcg tgaccctgcg agccataacc gatgatcgtg
     2461 acgctcttgc ccttgaccag gctgagatcg gcgtccttgt cgtagtagac cttcatgtga
     2521 tgttccggtt gattcagttt agatgcgcag gatgcgttcg ccccggccga tgccgctcga
     2581 gccggtgcgc accgtctcga ggatgacgtt cctgtcgatc gcctcgagga aggcgtcgag
     2641 tttctccgcc gctccggtga gctcgacggt gtagctcttc tcggtgacgt cgatgatgcg
     2701 gccgcggaag atctccgcca tccgcttcat ctcgtcgcgc tccttgccga cggcacggac
     2761 cttgatgagc atcagctcgc gctcggtata agcgccttcg gtgaggtcga ccaccttgac
     2821 cacctcgatc aggcggttga ggtgcttggt gatctgctcg atgacctcgt cggagccggt
     2881 ggtcacgatc gtcatccgcg acagggaagc gtcttcggtc ggagcgaccg tgagcgtctc
     2941 gatgttgtag ccgcgcgccg aaaagagcgc cacgacacgc gacagcgcac cgggctcgtt
     3001 ctcgagaagg accgcgatga tgtgcttcat cgcggcagcc tcgacgcatc gggccgctgc
     3061 aacgcgaggt tgcggcccgg gtgctgaccc ggggctgggc atgagttggc ggacttcatg
     3121 tcaaaggtct tccgagccga gcagcatctc gctgatgccc ttgccggcct gcaccatcgg
     3181 ccagacgttg gcggccgggt cggttcgcac gtcgaggaac accgtccgct ccttcaggcg
     3241 gatcgcctcc ttcagggccg gctcgacatc ggccggcttg tcgatgcgaa gaccgacgtg
     3301 tccgtaggcc tcggcgagct tgacgaaatc gggcagcgcg tccatgtagc tgtgcgagta
     3361 gcggccgccg tagtcgatct cctgccattg ccgcaccatg ccgaggtagc ggttgttcag
     3421 cgagacgatc ttcaccggcg tcttgtattg caggcaggtc gacagctcct ggatgcacat
     3481 ctggatcgag ccctcgccgg tgatgcagta gacgtcggca tcgggcctcg ccagcttgat
     3541 gcccatggcg tagggcaggc cgacgcccat cgtgccgagg ccgcccgagt tgatccagcg
     3601 ccgcggctgc tcgaagcggt agtactgggc ggcccacatc tggtgctggc cgacgtcgga
     3661 ggtgatgtag gtgtcgcggt ccttcgtcag ctgccagagg gtgtcgacga cgtgctgcgg
     3721 cttgatgaca tcgccgtcgc ccgcataagc gagacagtct cgctttcgcc attcgttgat
     3781 ctgcgcccac cacggcgaga gcgccttgag gtcgggccgc gacgccgatt ctcgaagaag
     3841 cgcagtcagc tcctgcagca cgtcgcgcac gtcgccgacg atcggcacgt cgacgcggac
     3901 gcgcttggag atcgacgacg ggtcgatgtc gatgtggacg atcttgcgct cgaccgacgc
     3961 gaagtgcttc gggttgccga tcacgcggtc gtcgaagcgc gcgccgatgg cgagcaggac
     4021 gtcgcagttc tgcatcgtca tgttcgcctc gtaggtacca tgcatgccca gcatgccgag
     4081 aaacttcgga tcgctcgccg ggtaggcacc cagccccatc agcgtgttcg tgcagggaaa
     4141 gccgagcagg tcgacgagct cgcgcagctc ggccgaagca ttgccgatga caaccccgcc
     4201 acccgcgtac acgtagggcc gcttcgctcc cagcagcagc tgggccgcct tgcggatctg
     4261 cccgccgtgg ccctttttca ccgggttgta cgagcgcatc tcgatcgtcg ccgggtagtg
     4321 gtacggcgtg gtggccatcg acacgtcctt gggaacgtcg acgacaacgg ggccgggccg
     4381 gccggtgcgc gcgatgtgaa aggccttctt catcgtcgtc gcgagatcgc ggacgtcctt
     4441 gacgaggaag ttgtgcttga cgaccggccg cgtgatgccg acggtgtcgc actcctggaa
     4501 ggcatcgagg ccgatcgccg gcgtcggcac ctggcccgtg atgatgacca tcgggatcga
     4561 gtccatgtac gccgtggcga tgccggtgat ggcgttcgtc acgcccggac ccgacgtgac
     4621 cagcgcgacg ccgacctcgc cggtcgcccg cgcgaagccg tcagcggcgt gaacggccgc
     4681 ctgctcgtga cgcacgagga cgtgctcgat ggtctgctgc ttgtaaagcg cgtcgtagat
     4741 gtagaggacg gcgccgcctg ggtagcccca caggtagcgg acgttttctg cctggaggca
     4801 acggacgagg atctcggagc cgttgggatc gccggccgga gcgggccgga gcgagggcgc
     4861 gggggcgcgc ttcagttcga gtgctgacgt ttccatgagt cgaaaacctc tgtgaatttc
     4921 tctgacgaaa aaccatcggc gcacctttcc tcgccctcgt gaggcgggtg cggtgcctgg
     4981 ggcgatcggc cggaccgccc gggatgcggg cgagtcctcg atcgaaactg tcgtgcaacc
     5041 gatgattatt gcatcggcag ccttcagacc ggccgcgccc gggcggtccg aagtggcgat
     5101 gtgataatcc gttcgcttcg acctgcggcc cgagggcttg ccttacttgg cgaccgacaa
     5161 agaacttgcc gattttctga aaagcgtcga gaagcgtgcc tggaagcgcg ccgtctacgc
     5221 ggtccgggac gaagacgcgg cgctcgacat cgtccaggac gcgatgatcc ggctgtcgga
     5281 aaagtacgtc gaccgaccgg ttgccgagct gccgctcctc tttcagcgga tcgtctcgaa
     5341 tgcgaccatg gactggtttc gccgccagaa ggtgcgcaac caggtcgtgc gcaatttctc
     5401 ggaattcgaa tcggcaggga acgacggcga cttcgacctg ctcgaaacgc tccaggcgct
     5461 cggcagctcg ctggagtccg agagcgccgc ggattcggtg tcccgggctc agatcttgca
     5521 agtcatcgac cacgaagtgg cgcagttgcc ggcacgtcaa cgcgaggcgt ttctcctgcg
     5581 ttactgggag gagctcgacg tcgccgaaac tgccgcctcg atgggttgtt ccgaaggcag
     5641 tgtcaagacc cattgctcgc gcgccgtgca cacgctcgcc aaggcgttgc aggccaaggg
     5701 aatttctcca tgaacacgct tgaatccacc taccgcacgg ccgcccggga ggcgatcgag
     5761 gcgcgcttcg cgcgcggcgt cgtggcgcgc ctgagcgaac aggccgacca cgtttcgccc
     5821 gaagtcggcg agcggctgcg tttcgcccgg cagaaggcgc tcgaggcggc gcaacgggca
     5881 cgcgcctccg gcgcggtcga ggcggccggt cgaagcggtc gctcggcaac cgctggattc
     5941 gcccgcgcct catggtggct gcgggtcgct tcggtgctgc cactggtcgc gctcgtcggc
     6001 ggcctcgtcc tgatccagga atcgcagacg cgctcgcaga tcacgacggc agcccaggtc
     6061 gacaccgagc tgctcggcga cgacctcccc ctcaacgcct atcgggacgc cggcttcgtc
     6121 gaattcctga aaacgccgga cagcgagtga ggtcgcacga catgcgagcg cggtctgccc
     6181 gaacggcgcg catcgctgcc gcatcggtgc tgctcgtcgg cgcctgcctg gtcgtcgcca
     6241 gccgggggat ggcgcagccg tccccgtccg cgcctgccgg acgccccgtt gccgtgccgc
     6301 cccacccggc agcggtcagg gccaccggcg gtccggttcg cccgcgcccc gaggaaggtg
     6361 tccgctggca gaagctcaac gcggcgcagc gccaggcgct cgcgccgctg gcccagcaat
     6421 ggtcgtcgat cgacgccccg cgcaagcaga aatggctggt catcgcccag cgcttcaatt
     6481 cgctgccgcc gctcgagcag gcccgcatca ccgaccggat gaccgcgtgg gcgaagctga
     6541 cgccggccga gcgcggcgag atgcgcctgc gctaccagga atcgcggcag gtccggggcg
     6601 atcgctcggc acgctggcag gcttaccagc agttgtcgcc cgagcagcgc aaggagttcg
     6661 aggcgcgtgc cgtgctgccg gccgtcgcgc cgaccgctct gcaaaagcgc gacacggcag
     6721 ggcggcaggg cagccccggc aagatcaaca tcgtcagcaa cccggcgctg gcgcaaccgc
     6781 cgcgtccggt cgcgccgacg gtcgtccagg cctcgcgcgg cgcgacgaca cacctgatca
     6841 cccggccggt ggcgccgccg gcgcaccagc agacaggcat gccgaagatc gaggcgacgc
     6901 ccgaattcgt caaccgctcg acgctgctgc cgcgtcgcgg accgcaggcg gccggcgtca
     6961 tgccgcccta catcccgatt cgtccgatgg ccgcgccccg ggtgcagccg gcggcgaccg
     7021 cagcgcatcc tggcaagacg cccgaacccg ccccggcgcg atgagcgagg ccccggccgc
     7081 ggcggcgatg cccggcgcgg cgccgaccac gccgtcgatc gcccggcgga tggcctgctt
     7141 cgtctatgaa gcgacgctgc tcttcggact gggactgatc ccgggcgtga tcggcgccgt
     7201 cttcgtcgcc cagaccggcc agcacggcgt gctgcagagc ccgaccgcgc ttcgcatcta
     7261 cgcctggatc ttgtacggcg tctacttcgt ctggttctgg acggtgcgcg gccagaccct
     7321 ggcgatgcag acgtggcgca tcggtctcgt cacggcggcc ggacaccttc cgacgcaggc
     7381 gcgggcgctg acgcgctacg tcgcttgctg catcgcctgg ttcgggccgt cgacggccat
     7441 cgcattcgcg ttgcgcctgc cgccgtcgac gagcctcgcg ctggcggccg cctggatcgt
     7501 cgtctacgcg ctgctcgcgc gggcggcacc cggccggcag ttctggcacg acatcgcctg
     7561 cggcacgcgc ctggtcgaca cacgcgccat cgactgggcc tcgctcagat cgcgtcctcg
     7621 ttgagctcgc cggtgcggat gcgcaccacg ctctcgattg ccgtcacgaa gatcttgcca
     7681 tcgccgatct tcccggtctt ggccgccttg acgacggctt cgatgcaacg ctcggcatcg
     7741 gcgtccttga cgacgacctc gaccttcacc ttgggcagga aatcgacgac gtattcggcg
     7801 cctcgataga gctcggtgtg ccccttctgc cggccgaagc ccttgacctc ggtcaccgtc
     7861 aggcccgaga cgccgatgtc ggcgagggcc tcgcgcactt cctcgagctt gaacggcttg
     7921 acgatggcgg tgatctgctt catggccggc tccggtgccc attcgatcgg tctagtttag
     7981 gccaaggccc taacatgcgg cgcgccatgc ccgcctcgaa gatgcccgcc aggaatgcgc
     8041 cacgcgcggc cgacgcggac gcatggaacg ttcgcgtctg cgtcgatccg ggcgagatcg
     8101 acgccgcggc ctgggacgcc ctcgtcgacg cgcagccgtc ggcgacaccc ttcatgacgc
     8161 tcgcctacct gcgcgcgctc catgcgtcgg gaagcgccag cgcagagacg ggctggtcgc
     8221 cgcatttcct cctgctcgag aacgccggcg agctgcttgc cgcgtgtccg ctctatctga
     8281 aggatcactc gtacggcgag tacgtgttcg actgggcctg ggccgacgcc tatcgccggc
     8341 acgggctcga ctactacccc aagctgctcg acgccgtgcc gttcacgccg gtgccggggc
     8401 cgcgcctgct ggcacgcgac cccgagcatc gtctcgtgct gctgcgcggc atcgagcagc
     8461 tcgcgcggtc cgccgggctg tcgtcggcgc acctgctctt tctcgacgcc gacgaccagg
     8521 cggcggcgcg cgccgccgga tggtcgatgc gcacgaccgt ccagttccac tggcacaacc
     8581 gtgagcccac gccctggctc gacttcgccg acttcctcgc cagcctgcag cgcgaaaagc
     8641 gcaagaagat ccagcaggag cgacgacgag tcgccgaagc cggcgtcacc ttcaccgcgt
     8701 cggtcggacg cgccatctct gacaccgact gggatttctt ctatcgctgc tacaccctca
     8761 cctatcgcgc ccaccactcg acgccgtacc tgacccggga tttcttcgct cgcgccgcca
     8821 gcgacctggc ggatcactgg ctgctcttcg tcgcttatcg ccaaggcagg ccgatcgctt
     8881 cttcgctcgt cgtcatcgac ccgcaacgaa aggccgcctt cggtcgctac tggggcgccg
     8941 tcgagcacgt cagctgcctg cacttcgacg cctgctacta cgagccgctc gcctggtgca
     9001 tcgcccacgg ctatcgacgc ttcgaaggcg gcgcgcaggg cgagcacaag atggcgcgcg
     9061 gactgctgcc ggtgcagacg ttctcggcgc actggctcgc gcatccgcag ttcgcgcgcg
     9121 cgatcgacga cttcctcgcc cgcgagggcg ccggcatcga ggagtacgtc ggcgagctga
     9181 acgagcgcct gccgttcaag ccgccgccgg gctagagcgt cgcggcgcct gccgacgtcc
     9241 gcgggtgctg gcgcgcgtag ctggcgatgc cctggcggat ctcgtcgcgc gccgcgtcgg
     9301 gaccgtccca gcccatcgtc ttcacccact tgcccgcctc gaggtccttg tagtgctcga
     9361 agaagtgctg gatctgcttc aggcgcagct cgttcatgtc ggcgggcttt tgccaatgcc
     9421 ggtagatcgg caggatcttg tcgatcggca ccgcgagcag cttggcgtcg ccgcccgc
//
LOCUS       DASRWS010000206         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3254619, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000206 DASRWS010000000
VERSION     DASRWS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3254619"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="lexA"
                     /locus_tag="VFF72_03835"
     CDS             <1..649
                     /gene="lexA"
                     /locus_tag="VFF72_03835"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZV92321.1"
                     /translation="RQQQILDLVQSAIERTGAPPTRAEIANELGFKSANAAEEHLQAL
                     ARKGVIELIGGTSRGIRLQSDTLRELNKARGKQFSLPLPSLAQLSLPLIGRVAAGSPI
                     LAQEHVDRTFLMEASLFSRRPDYLLKVRGMSMKDAGIMDGDLLAVQKAHEAKNGQIVV
                     ARLGDEVTVKRFRRSRGTVELLPENPDFETIVVGPEDVFAIEGLAVGLVRNHMLM"
     gene            657..956
                     /locus_tag="VFF72_03840"
     CDS             657..956
                     /locus_tag="VFF72_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZV92322.1"
                     /translation="MVIKSPKAASAAECQRLEQLPNVGPAIAADLRRIGIAHPADLRG
                     RDAFVLYRRLCENKGHRQDPCVLDTFLAVADFMRGAPAAPWWKYTARRKAAFGDL"
     gene            1046..1492
                     /locus_tag="VFF72_03845"
     CDS             1046..1492
                     /locus_tag="VFF72_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92323.1"
                     /translation="MAANNGGTGQSLPVGHVVMSPIRFLLSSLMPSVPTDTRSRRAES
                     CPPLARPRHRPWPLRLGGRAGRMLAAARLDFADALDDIRNAASLDAKTRIAVTRSLHE
                     LWHLREEVFSLVARRHDQSEASRRLARLNRHFLRRGGDSLRPFERS"
     gene            complement(1527..2036)
                     /locus_tag="VFF72_03850"
     CDS             complement(1527..2036)
                     /locus_tag="VFF72_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZV92324.1"
                     /translation="MSISMHSASVPIFVRMLGNMTRWLDKAEAHAKERKFDAANYLGA
                     RLAPDMLPFTKQIQIACDSAKFCVARLAGSEAPKFEDDEASLADLRERIRKTVDYVNS
                     VPASAIDGTDAKDIAVPRRDGTLTMKGEAYLKQFVLPNFFFHVTTVYALLRHNGVDLG
                     KSDYLGALG"
     gene            2145..>2664
                     /locus_tag="VFF72_03855"
     CDS             2145..>2664
                     /locus_tag="VFF72_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV92325.1"
                     /translation="MNIIILDDYQDAVRKLKCAAILEQLNAKVFTNTVKGIGQLSVRL
                     RDAEVLVLIRERTQFPRQLIEKLPKLRLIAQTGRVGPHIDIDTCTRLGVAVAEGVGSP
                     VAPAELTWALIMAAMRRLPHYISTLKHGAWQQSGLKAASMPPNFSLGLVLKGRTLGIW
                     GYGRIGQIVAGYG"
BASE COUNT          431 a          943 c          873 g          417 t
ORIGIN      
        1 tcgccagcag cagattctcg atctggtgca aagcgccatc gagcgcaccg gcgctccgcc
       61 gacacgcgcc gagatcgcca acgagctcgg cttcaagtcg gccaacgcgg ccgaggagca
      121 tctgcaagcg ctggcgcgaa agggcgtcat cgagctcatc ggtggcacgt cgcgcggcat
      181 ccggctgcag tctgacacgt tgcgcgagct caacaaggcg cgcggcaagc agttctcgct
      241 gccgctgccg agcctggcgc agctgtcgct accgctgatc ggccgggtcg ccgccggcag
      301 cccgatcctc gcccaggagc acgtcgatcg cacctttctc atggaggcga gcctgttctc
      361 ccgacggccc gactacctgc tcaaggttcg cggcatgagc atgaaggacg ccggcatcat
      421 ggacggcgac ctgctcgccg tgcagaaggc ccacgaagcc aagaacggcc agatcgtggt
      481 cgcccgcctg ggcgacgagg tcaccgtcaa gcggttccgg cgcagtcgcg gcacggtcga
      541 gttgctgccc gaaaatcccg acttcgagac catcgtcgtc gggcccgaag atgtcttcgc
      601 catcgaaggc ctggccgtcg gcctcgtccg caaccacatg ctgatgtgag cgggcgatgg
      661 tgatcaagtc gcccaaggca gcgagcgcgg cagagtgcca gcgcctcgag caactgccca
      721 acgtcgggcc ggcgattgcc gccgacctgc gccgcatcgg catcgcccat ccggccgacc
      781 tgcgcggccg cgacgcgttc gtcctctatc ggcgcctctg cgagaacaag gggcaccgcc
      841 aggatccttg cgtcctcgac accttcctgg ccgtcgccga tttcatgcgc ggtgcaccgg
      901 ccgcgccgtg gtggaaatac acggcccgcc gcaaggccgc attcggcgat ctgtgaggca
      961 acgcgtcggt cgcggcagac cggggtagac cggcccgggc gaacaaggcg ctccaggcag
     1021 cgcttttcag cctttcctcg tcggcatggc cgcgaacaat ggcggcacgg ggcagagcct
     1081 gcccgtcggc cacgtcgtca tgtcaccgat tcgattcctg ctgagctcgc tgatgccgtc
     1141 cgtgccgacc gacacgaggt cgagacgcgc cgaaagctgc ccgcccctgg cccgtccccg
     1201 gcaccggccc tggccattgc gcctcggcgg acgggccggc cggatgctgg ccgcggcccg
     1261 cctcgatttc gccgatgccc tcgacgacat ccgaaacgcc gcgtcgctcg acgccaagac
     1321 ccggatcgcg gtgacgcgct ccctgcacga gctctggcat cttcgcgagg aagtcttcag
     1381 cctcgtcgcg cggcgccacg atcaatcgga agcgagccgg cgtctggccc ggctgaatcg
     1441 ccatttcctt cgccgcggcg gcgactcgct tcgcccgttc gagcgctcct gatcgacggc
     1501 gaccgcagcg ccccgcccgc gccgactcag ccgagcgcgc cgagatagtc cgacttgccg
     1561 aggtcgacgc cgttgtggcg cagcagcgca tacacggtcg tgacgtggaa gaagaaattc
     1621 ggcagcacga actgcttcag gtaggcctcg cccttcatcg tcagggtacc gtcgcgtcgc
     1681 ggcacggcga tgtccttcgc atcggtgccg tcgatggccg acgccggcac cgaattcacg
     1741 tagtcgaccg tcttcctgat ccgctcgcgc aggtcggcga ggctggcctc gtcgtcctcg
     1801 aacttcggcg cttcgctgcc ggcaagccgg gcgacgcaga acttcgccga gtcgcaggcg
     1861 atctggatct gcttggtgaa cggcagcatg tcgggggcga ggcgcgcgcc gagatagttt
     1921 gcggcgtcga acttgcgctc cttggcatgg gcttctgcct tgtccagcca tcgggtcatg
     1981 ttgccgagca tgcgcacgaa gatcggcacg ctcgctgaat gcatcgagat cgacacgtcg
     2041 ttctccaggt gagaggaatc gcggcatgct acccgcgacc gccgagacgt gcacaatcga
     2101 cggccggggc gcctgcgcgg cgcgccgagc cggtatcgcg acccgtgaac atcatcatcc
     2161 tcgacgacta ccaggatgcg gtgcgcaagc tcaagtgcgc ggcgatcctc gagcaactca
     2221 acgccaaggt cttcaccaac accgtcaagg gcatcggcca gctgtcggtg cggttgcgcg
     2281 atgccgaggt gctggtgctg atccgcgagc gcacccagtt cccgagacag ctgatcgaga
     2341 agcttcccaa gctgaggctg atcgcccaga ccggccgggt cggcccgcac atcgacatcg
     2401 acacctgcac ccgcctcggt gtcgccgtgg ccgaaggcgt cggctcgccg gtcgcgcccg
     2461 ccgagctgac gtgggcgctg atcatggcgg caatgcggcg gctgccgcac tacatcagca
     2521 cgctcaagca cggcgcgtgg cagcagtcgg gcctgaaggc ggcgtcgatg ccgcccaact
     2581 tctcgctcgg actggtgctc aagggccgca cgctcggcat ctggggctac ggccgcatcg
     2641 ggcagatcgt cgccggctac ggcc
//
LOCUS       DASRWS010000207         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_944777, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000207 DASRWS010000000
VERSION     DASRWS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944777"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..512)
                     /locus_tag="VFF72_03860"
     CDS             complement(6..512)
                     /locus_tag="VFF72_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZV92326.1"
                     /translation="MNDNTDSRAHLWKLVKDIKFAMFTTRHGNGHLHARPMTTQNKNG
                     IEADESLWFFMSKKGDPVDDLKADPVVCVSYADPSKDTYVSVSGTAAMLQDQAKKDQL
                     WSKMAEAWFPGGSTDPDLALIQVQIVHANYWDVKESKLVQLFTMARAAVTGKPPTELG
                     EHGVVRMR"
     gene            complement(628..2037)
                     /locus_tag="VFF72_03865"
     CDS             complement(628..2037)
                     /locus_tag="VFF72_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426331.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HZV92327.1"
                     /translation="MQPHEDAPYAPPAASAGVPANDAGIRGVTLAAPTDLAGFRRACR
                     SLWSAQIAPERVVWRVAGEPRADLFETEPVASTRAAPPVRVPAAFLAQCESAILHNDA
                     GRFGLLYRLLWRLQIEPALRHDALDADRVAVDAMAQAVRRDMHAMQAQVRFSRVASDA
                     AASPTHVAWYAPEHHIVEATAPLFAQRFSQLRWVIATPDGSVRWDGQRLHFGKAARRD
                     QWPSVDADESAWRVFCQALFARTTDGPDSLMSDVAPGSSSHAMRPQPLATKSGPATPA
                     SLGALVDALQRCRECPIGEFATQAVPGEGSRHARLMFVGEQPGDQEDLQGRPFVGPAG
                     QLFDRALADLDIDRRTAYVTNAVKHFKFELRGKRRIHKTPAQQEAAACLHWLETEIAL
                     VDPGAIVALGATAARQLMGTAVAVTRQRGRWLKRPDGRQVLVTLHPSALLRMDPQDKD
                     DAYGAWLADLRKAADRSRP"
     gene            complement(2089..>2481)
                     /locus_tag="VFF72_03870"
     CDS             complement(2089..>2481)
                     /locus_tag="VFF72_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZV92328.1"
                     /translation="FLADFVLYFAKLFDGLRHDSGDPFVWGEKALRHYDKLRIDPKTK
                     RLVFSDGLDFDKAFALYRHFADRTMVGFGIGTHLTNDMGLKTLHIVMKLTHANRQPVA
                     KLSDSPGKLLCDDETFLAYLRQVFKVPA"
BASE COUNT          387 a          816 c          859 g          419 t
ORIGIN      
        1 gccgctcagc gcatgcgaac cacaccgtgc tctccgagct cggtcggcgg ttttccggtg
       61 acggccgccc tggccatcgt gaacaactgg acgagcttgc tttccttcac gtcccagtag
      121 ttcgcgtgga cgatctgcac ctggatcagg gccagatcgg ggtcggtgga accgccggga
      181 aaccaggcct cggccatctt gctccacaac tggtccttct tggcctggtc ctggagcatc
      241 gccgcggtgc cggacaccga cacataggtg tccttcgacg gatcggcgta cgagacgcat
      301 accaccggat cggccttcag gtcgtcgacg ggatcgccct tcttcgacat gaagaaccag
      361 aggctttcgt cggcctcgat gccgttcttg ttctgcgtcg tcatcggccg ggcatgcaaa
      421 tggccgttgc cgtgtcgggt cgtgaacatc gcgaacttga tgtccttgac gagtttccag
      481 aggtgcgcgc gtgagtcggt gttgtcgttc atgggatccc ggtcgataga tgccgcaaag
      541 gcgttgactg cattgaagca tcgttgccgc gaacagccgg tcgggcaggg cgcgcccgcg
      601 ctgtaggact ctttcggatc ggcggcctca gggccgagac ctgtcggcgg cctttcgcag
      661 gtcggccagc caggccccgt acgcgtcgtc cttgtcctgc ggatccatcc tcagcagcgc
      721 cgacggatga agcgtgacca gcacctgcct gccatcgggg cgcttcagcc agcgaccacg
      781 ctggcgtgtc accgcgacgg ccgtgcccat caactggcgc gccgcggtcg ctccgagcgc
      841 gacgatcgcg ccgggatcga cgagcgcgat ctcggtctcg agccagtgca ggcacgccgc
      901 ggcttcctgc tgcgccggcg tcttgtggat gcgtcgcttg ccgcgcagct cgaacttgaa
      961 atgcttgacc gcattggtga cgtaggccgt gcgccgatcg atgtcgaggt cagccagggc
     1021 gcggtcgaaa agctgtcctg ccggtccgac gaaaggccgg ccttgcaggt cctcctgatc
     1081 gcccggctgt tcgccgacga acatcagccg cgcatgtcgg ctcccctcac ccgggacggc
     1141 ttgggtcgcg aactcgccga tcgggcattc gcggcagcgt tggagcgcgt cgacgagcgc
     1201 cccgagcgag gcaggcgtgg ccgggccgga cttcgtggcg agtggctggg gacgcatcgc
     1261 gtggctcgac gaaccagggg cgacatcgct catgaggctg tccggcccgt cagtggtcct
     1321 tgcgaagaga gcttggcaaa agacgcgcca ggcgctttcg tccgcgtcga ccgaaggcca
     1381 ttgatcgcga cgcgccgcct tgccgaagtg aagccgctgg ccgtcccatc gcacgcttcc
     1441 gtccggggtc gcgatgaccc agcggagctg gctgaaacgc tgcgcgaaaa gcggcgccgt
     1501 cgcctcgacg atgtgatgct ccggagcgta ccacgccacg tgcgtcggtg aagccgcggc
     1561 gtcgctggcg actctcgaga agcggacctg cgcctgcatc gcgtgcatgt cccgccgcac
     1621 cgcctgcgcc atcgcatcga ccgcgacacg atcggcgtcg agcgcgtcgt ggcgcagcgc
     1681 cggctcgatc tgcagccgcc agaggagccg atagagcagg ccgaagcggc cggcgtcgtt
     1741 gtgcaggatc gcgctctcgc actgcgcgag aaaggccgcc gggacccgca ccggcggcgc
     1801 ggcccgcgtc gaagcgacgg gctcggtttc gaacaggtcc gctcggggct cgccggccac
     1861 tcgccagacg acccgctcgg gcgcaatctg cgccgaccac agcgaccggc aggcgcgccg
     1921 gaagccggcc agatcggtcg gagcggccag ggtgacgccg cggatcccgg catcgtttgc
     1981 cggtacgccc gcgctggcgg ccggcggcgc atacggcgca tcctcgtgcg gctgcatgtc
     2041 gctagcgtag cgcgtcggcg gagcccggcc gcggcttgac ggacttgtct acgcagggac
     2101 cttgaacacc tgtcgcaagt aggcgaggaa ggtctcgtcg tcgcagagga gcttgcccgg
     2161 actgtcagac agcttggcca ccggttgccg gttggcatgc gtcagcttca tgacgatgtg
     2221 cagcgtcttc aggcccatgt cgttggtgag atgggtgccg atgccgaagc cgaccatggt
     2281 ccggtcggcg aaatgccggt agagcgcgaa cgccttgtcg aagtcgaggc cgtcggaaaa
     2341 gaccagccgc ttggtcttgg ggtcgatgcg cagcttgtcg tagtggcgca gcgccttctc
     2401 gccccagacg aacgggtcgc ccgagtcgtg gcgcaggccg tcgaagagct tggcgaagta
     2461 gagaacgaaa tcggccagga a
//
LOCUS       DASRWS010000208         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_376177, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000208 DASRWS010000000
VERSION     DASRWS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376177"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFF72_03875"
     CDS             <1..306
                     /locus_tag="VFF72_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV92329.1"
                     /translation="EGGFALKRSAFFREQFRRQLETGQKAKGVDLWEAVQRIKAPTLA
                     LRASRSDLFSAETVPKLLAANPRIEFREIEGGHNLAGDNPQATLAVMRDFIDSLENT"
     gene            303..887
                     /locus_tag="VFF72_03880"
     CDS             303..887
                     /locus_tag="VFF72_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV92330.1"
                     /translation="MTRTQFDATPLRGIDHLAFVTDDLPTTMDFYTRVMQMRLVHVRR
                     VPFEKDRGQPPYDNLRHYFFDMGHDQLLAFFEYPKGLPRQDRDRPGGMQHIAFHVPPE
                     RFDALVEHVRSEGVDVVGPVALGGRFWSAYFYDPNGIRLELATSRLEPYGPVLDSVYQ
                     TQQEAREELQTLFDDPAVVDRWLSRMPLKDTRPA"
     gene            975..1919
                     /locus_tag="VFF72_03885"
     CDS             975..1919
                     /locus_tag="VFF72_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92331.1"
                     /translation="MLLSTTAAAFGSSPFAAMGADDVVKALVGFPPGGAIDTTARAFG
                     RAMTSVGTVVVENHPGAAGNIAAGLLAQSRPDGRTLMFAPVNVYCISQALYRNLAFDA
                     VRDFAPVGITATFPWSLAVHPSVPAHSLSEFVAWLKANPQKALCGMAAIGSEGHLMAY
                     AFSKEAGIPLQFVPYKGGAPMAQDLMAGQILMAFDPIVNMGPPHRAGKVRILAVTGRQ
                     RAASLPEVPTFSELGFPAITGQTWIGVAARKGTPSETIQSYSSALIAAANEPSLRERL
                     ALVGLTAVAGTPDEMGAQMAADTARYGKLARELGLRLD"
     gene            complement(2206..2934)
                     /locus_tag="VFF72_03890"
     CDS             complement(2206..2934)
                     /locus_tag="VFF72_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HZV92332.1"
                     /translation="MPDALTILSLLIEQIAHCLDEPSLVDLLRQTSFLTGAEAAFFAS
                     WFCQGTALASCHFLLACDPRWGLAYDEACCLDADPWLRYAMRHSAPLPSDRIACMDER
                     ERAVVELATSFGFHSAALVPAPAPSGSTHGGLLVLGSSRPAFFEGGRFHDLKVIARNI
                     AMELQERHADLLRAAFVRERHITPADLRLLARAREGTSTKALAGFEGLSPGAVDARFR
                     RLNAKFGSPNRETTARLAAGLGLL"
BASE COUNT          547 a          982 c          986 g          523 t
ORIGIN      
        1 gagggaggct tcgcgctgaa gcgaagcgcg tttttccgcg aacagtttcg ccgacagctc
       61 gagactggcc agaaggccaa gggcgttgat ctgtgggagg cggtgcaacg aatcaaggct
      121 ccgacgctcg cgttgcgggc gtcccgttct gatctgttca gcgccgagac agtgcccaag
      181 ttgttggctg ccaacccgcg aatcgaattt cgcgaaatcg agggcggcca caacctcgcg
      241 ggcgacaacc cgcaagccac gttggccgtc atgcgcgact tcatcgacag tctggagaac
      301 acatgactcg aacgcaattc gacgcgacgc ctctgcgcgg catcgaccat ctcgccttcg
      361 ttactgatga cttacctacg acgatggatt tctacacgcg cgtgatgcag atgcgactcg
      421 tgcacgtacg ccgtgtccca ttcgagaaag accgcggcca gcctccctac gacaacctgc
      481 gccactattt cttcgacatg ggccacgatc agttgctggc cttcttcgag tacccgaaag
      541 ggctaccgcg ccaggatcgc gaccgtccgg gcggcatgca gcacatcgcc ttccacgtcc
      601 cgcccgagcg gttcgatgcg ctcgtggaac acgtccgatc cgaaggggtc gacgtcgtcg
      661 ggccagtggc gcttggcggc cgcttctggt cggcctactt ctacgacccg aacggcatac
      721 ggctcgagct ggcaacgagc cggctcgagc cgtacggccc ggtgctcgac tccgtctacc
      781 agacccagca agaggcacga gaagagttac agacgctctt cgacgacccg gccgtggtcg
      841 accgctggct ctcccgcatg ccgctcaagg acacccggcc ggcgtagctg gcgagcgcgg
      901 cgcaaggcga cattcaatga cacgaggaga cgagcatgag taggacgacg acatcccccg
      961 tgacccggcg agccatgctg ctgtcgacga cggcagccgc gtttggcagc agtcccttcg
     1021 cggcaatggg ggccgacgat gtcgtcaagg cgttggtcgg ctttcctccg ggcggcgcca
     1081 tcgacaccac ggcgcgggcg ttcggtcgcg ccatgacaag cgttggcact gtggtcgtcg
     1141 agaaccatcc gggagccgcg ggaaatattg ccgcggggtt gcttgcgcaa tctcggcctg
     1201 acggccgaac gctgatgttc gcgccggtca atgtctactg catttcgcag gcgctctacc
     1261 gcaatctcgc cttcgatgcc gtgcgcgatt ttgcgcccgt cggaatcact gcaacgttcc
     1321 cctggtcctt ggccgtccac ccaagcgtgc cggcccattc actttccgaa tttgtcgcat
     1381 ggctgaaggc caatccgcag aaggcgctct gcggcatggc tgccattggc agtgagggtc
     1441 atctcatggc ctatgcgttt tccaaggagg ccggcatccc gctgcaattc gtcccttaca
     1501 agggcggcgc gccgatggcc caggacctca tggcgggcca gatcctcatg gccttcgatc
     1561 caatcgtcaa catggggccg cctcaccgtg ccgggaaggt gcggatcctc gccgtgaccg
     1621 gccgtcagcg cgctgcctcg ctacccgaag taccgacgtt cagtgaactt ggcttccctg
     1681 cgatcacggg tcagacctgg atcggcgtag cggcccgcaa gggcacgccc agcgagacga
     1741 tccagtcgta ttcgagcgcg ctcatcgctg cggcaaacga gccgtcactc cgcgagcgtc
     1801 ttgccctggt cggattgact gccgtcgctg gaacaccgga tgagatgggc gcccagatgg
     1861 ctgcggatac ggcgcggtac gggaagctgg cccgagagct tggtctccgg ctcgactgag
     1921 agcccgtcgc cggccgatga agctgactgt gttggccaag caggcgcgca tcgaggccat
     1981 gaaaaaaggg cgaatcgcta tttggtgagc ttcgtgcggt cagggttcga accgcaggat
     2041 cccgagaccg gtctgtcgtg atcgcgctgc acgacgcaac ccggctgtca cacacacggg
     2101 gacacgaacg cgtgcggttc agtcgtcccg gggagacttc ttgacactgc atccgggcgt
     2161 tgcgccgtcg gcgtcagctg ttgagacgcc ctggcttgat cgtgatcaaa gcagtcccag
     2221 gccggccgcc aagcgtgcag tcgtttcccg gttcggtgag ccgaacttcg cgttgagccg
     2281 ccgaaatcgg gcatcgacgg cgccgggcga caggccttcg aatcccgcca gggccttcgt
     2341 cgatgtccct tcgcgggctc gcgccaggag gcgcaggtcc gccggggtga tgtgacgctc
     2401 cctgacgaag gcggcgcgga gcagatctgc gtgtcgctcc tgcagctcca tcgcaatgtt
     2461 tctggcgatg accttcagat cgtggaacct accgccctcg aaaaacgccg ggcgcgaaga
     2521 gcccaggacg aggagtccgc cgtgggtcga gccgctcggc gccggcgccg gaacgagcgc
     2581 ggcgctgtgg aagccgaacg aggtcgcaag ctcgaccacg gcacgctcgc gctcgtccat
     2641 gcatgcaatg cggtcactgg gaagcggtgc tgagtgccgc atcgcgtaac gcagccaagg
     2701 atccgcgtcg aggcagcagg cctcgtcata cgccagtccc caacgcggat cgcaggcgag
     2761 caggaagtgg caggaggcaa gggccgtgcc ttggcagaac caactcgcaa agaacgcggc
     2821 ttcggcgccg gtcagaaacg acgtttggcg cagcaggtcg accagcgatg gctcgtcgag
     2881 gcagtgcgcg atctgctcga tcaagaggct caggatcgtc agggcatcag gcatctacgc
     2941 ctccggccag cgccggcgtg gcatcgaagg cggcgatgcc gcacttcttt acctgatgcc
     3001 agagcagggg atcggcgaag cgcgtcgggt cgacctcg
//
LOCUS       DASRWS010000209         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3533505, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000209 DASRWS010000000
VERSION     DASRWS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533505"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFF72_03895"
     CDS             complement(<1..210)
                     /locus_tag="VFF72_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92333.1"
                     /translation="MTLARAALAESLLAARDGGRLIALPSEGESAFGLADAYAVADEI
                     RRLRIARGEKSVGYKIGFTNRSIWDR"
     gene            complement(207..1502)
                     /gene="serS"
                     /locus_tag="VFF72_03900"
     CDS             complement(207..1502)
                     /gene="serS"
                     /locus_tag="VFF72_03900"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZV92334.1"
                     /translation="MLDISLLRKDLASVVARLETRKCPQPFLDVDRFTALESERKSLQ
                     VRTEELQARRNALSKEIGKLKAQGADASAVMAEVGGIGDELKRCAERLEQVLADLDAQ
                     LMALPNLPDASVPVGADESANVEIRRWGAPPIFDFAVRDHVDVGAPLGLDLDIGAKLS
                     GSRFVFLRGPVARLHRALAQFMLDVQTEEHGYVECWTPYIVNREVLEGTGQLPKFKDD
                     MFWVTRGGDDALPEQYLISTSEISLTNSVRQQVLAEAELPIKLTAHTPCFRSEAGAAG
                     RDQRGMIRQHQFDKVEMVQIVRPERSAEALEEMVGHAENILRRLGLPHRVVLLSSGDM
                     GFGAAKTYDLEVWVPAQRTYREISSVSNCEAFQARRMQTRFKTAQGRNELVHTLNGSG
                     LAVGRTLVAVLENFQRADGSVAVPAPLRPYLGGLDELRP"
     gene            complement(1588..1806)
                     /locus_tag="VFF72_03905"
     CDS             complement(1588..1806)
                     /locus_tag="VFF72_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZV92335.1"
                     /translation="MIVCICRRVTDHQIRRAAAEGAVSLECLQLELGVAMQCGRCADC
                     ATQVLCEARASTPAGGTARHANLVEQMA"
     gene            complement(1834..2313)
                     /gene="bfr"
                     /locus_tag="VFF72_03910"
     CDS             complement(1834..2313)
                     /gene="bfr"
                     /locus_tag="VFF72_03910"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZV92336.1"
                     /translation="MKSDPQVIVHLNAQLKNELTATNQYFLHYRMLKHWGFDRLAKKE
                     YEESLGEMKHADRLMERIFMLDGLPNLQDLGKLLIGETAPEILDCDLRSETGAQATIK
                     DGIAYCESVRDYVSRDILQHILDDTEEHIDWLETQIELLGKVGAENYLQSQMGVQDS"
     gene            2407..2682
                     /locus_tag="VFF72_03915"
     CDS             2407..2682
                     /locus_tag="VFF72_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZV92337.1"
                     /translation="MIASSVARPLSALAAGARATVARVVAASPDIDAVALRRLGELGF
                     LPGEPVQVLRRGPGGREPLAILIGETIFALRLLEAQCIEVTPESPAR"
     gene            2697..>3040
                     /locus_tag="VFF72_03920"
     CDS             2697..>3040
                     /locus_tag="VFF72_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HZV92338.1"
                     /translation="MSTRTLDAANHAVPWQIALVGNPNCGKTALFNLLTGARQKVANY
                     AGVTVERKVGTTRLKNGRSVSIIDLPGAYSLTPATPDEQVTLEVIEGRRHGEAAPDAI
                     VAVVDATNLRMNL"
BASE COUNT          457 a         1031 c         1032 g          520 t
ORIGIN      
        1 gcggtcccag atgctccggt tggtaaagcc gatcttgtag cccaccgact tttcgccgcg
       61 ggcgatgcga aggcgccgga tctcgtcggc cacggcgtag gcatcggcga gcccgaacgc
      121 cgattcgccc tcgctcggca gcgcgatcag ccggccgcca tcgcgcgcgg caaggagcga
      181 ctcggccagc gccgctcggg ccagcgtcac ggtcgcagtt cgtcgaggcc gccgaggtac
      241 ggacgcagcg gcgccggaac cgcgacgctg ccatcggcgc gctggaagtt ctcgagcacg
      301 gcgacgagcg tgcggccgac cgcgaggccc gagccattga gcgtgtgcac gagctcgttc
      361 ctgccctgag ccgtcttgaa ccgcgtctgc atgcgccgcg cctggaaggc ctcgcagttc
      421 gagaccgagc tgatctcgcg gtatgtgcgc tgcgccggca cccagacctc gaggtcgtag
      481 gtcttcgccg cgccgaagcc catgtcgccg ctcgacagga gcacgacgcg atgaggcagg
      541 ccgagccggc gcaggatgtt ctcggcgtgg ccgaccatct cctcgagcgc ttcggcactt
      601 cgctcggggc gcacgatctg caccatctcg accttgtcga actggtgctg gcggatcatg
      661 ccgcgctggt cgcggcccgc cgcgccggcc tccgagcgaa agcacggggt gtgcgcggtc
      721 agcttgatcg gcagctccgc ctccgccagc acctgctggc ggaccgagtt cgtcagcgag
      781 atctccgagg tcgagatgag gtattgctcg ggcaaggcgt catcgccgcc gcgcgtcacc
      841 cagaacatgt cgtccttgaa cttgggcagc tggccggtgc cctcgagcac ctcgcggttg
      901 acgatgtagg gcgtccagca ttcgacgtag ccgtgttcct cggtctgcac gtccagcatg
      961 aactgcgcga gggcgcgatg caggcgcgcg accggcccgc gcaggaagac gaaccgcgac
     1021 ccggagagct tggcgccgat gtcgagatcg aggccgagcg gcgcgccgac gtcgacgtgg
     1081 tcgcgcaccg cgaagtcgaa gatcggcggc gcgccccagc gtcgaatctc gacgttggcc
     1141 gactcgtccg cgccgaccgg cacgctcgca tcgggcaggt tgggcaacgc catgagttgc
     1201 gcgtcgagat cggcgagcac ctgctcgagc cgctcggcac agcgcttcag ctcgtcgccg
     1261 atgccgccga cctcggccat cacggcggat gcatccgcgc cttgcgcctt cagcttgccg
     1321 atttccttcg acagcgcgtt gcgacgggcc tgcaactcct cggtgcgtac ctgcagcgac
     1381 ttgcgctccg actcgagtgc cgtgaagcga tcgacgtcga gaaaaggctg cgggcacttt
     1441 cgggtttcca ggcgtgcgac gacgctcgca agatccttgc gcaggaggct gatgtcgagc
     1501 atggcgatga cgccgcagac gcggcgccgc gaagtgcaca gggctccgga ttgtagggag
     1561 cgagcctgga gcgacggcgt gcagccctca ggccatctgc tcgacgaggt tcgcgtggcg
     1621 cgcggtgccg cctgccggcg tcgacgcccg cgcttcgcac agcacctgcg ttgcgcagtc
     1681 cgcgcatcgg ccgcactgca tggccacgcc gagctcgagt tgaaggcact ccaggctcac
     1741 cgcgccctcg gccgcggcgc ggcggatctg gtggtcggtg acccggcggc agatgcagac
     1801 gatcatgtgt attccagcgt cgatgcgatg cgttcagctg tcttgaacgc ccatctgcga
     1861 ctgcagatag ttctcggcac cgaccttgcc gagcagttcg atctgcgtct ccagccagtc
     1921 gatgtgctct tcggtgtcgt cgagaatgtg ttgaaggatg tcgcgcgaga cgtagtcgcg
     1981 caccgattcg caataggcga tgccgtcctt gatggtcgcc tgcgcccccg tctccgagcg
     2041 caggtcgcag tcgaggatct cgggcgccgt ctcgccgatc agcagcttgc cgaggtcttg
     2101 caggttcggc aggccgtcga gcatgaagat gcgctccatc agcctgtcgg cgtgcttcat
     2161 ctcgcccagc gattcttcgt attccttctt ggccaggcgg tcgaagcccc agtgcttgag
     2221 catccggtag tgcaggaagt actggttggt cgccgtcagc tcgttcttga gctgggcgtt
     2281 gagatggacg atcacctgcg ggtcgctttt catgggtagg tgctccggcg gctcgattat
     2341 gagtgataat ggttcctatt tgcaatccat ggcccgcgtt ggccggatcg catgcaggac
     2401 ttccgcatga tcgcctcttc cgtcgcacgt ccgctgagcg ccctcgcggc cggcgcgcgg
     2461 gcgaccgtcg ctcgcgtcgt cgccgcatcg cccgatatcg atgccgtcgc gttgcgccgc
     2521 ctcggcgagc tcggcttcct gccaggcgag ccggtgcagg tgctgcgccg cggcccgggc
     2581 ggccgcgagc ccctcgccat cctcatcggc gagaccatct tcgcgctgcg tctgctcgaa
     2641 gcgcagtgca tcgaagtgac tccggagtcg ccggcccgct gagccggcag ccactcatgt
     2701 cgacgcggac cctcgacgct gccaaccatg cggtgccgtg gcagatcgcc ctggtcggca
     2761 atccgaactg cggcaagacg gcgctgttca acctgctgac gggggcgcgg caaaaggtcg
     2821 ccaactacgc cggcgtgacg gtcgagcgca aggtcggcac gacgcggctg aagaacggca
     2881 ggtcggtctc gatcatcgac ctgcccgggg cgtacagcct gacgccggcc acgcccgacg
     2941 agcaggtcac gctggaggtg atcgagggtc gccgtcacgg cgaagcggcg cccgatgcga
     3001 tcgtcgccgt cgtcgacgcc accaatctgc gcatgaacct
//
LOCUS       DASRWS010000210         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2959947, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000210 DASRWS010000000
VERSION     DASRWS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959947"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VFF72_03925"
     CDS             complement(<1..1199)
                     /locus_tag="VFF72_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZV92339.1"
                     /translation="MATAALDVLREVCKPALLPDAALERVDLTGADPVLPSSFAIGAA
                     AQASLAAAALAASEVGRLRNGLVQRVSVDMVDAAVEARAAFLVDGNAPNVWDPIAGLY
                     PCADGWVRVHTNFAHHRDGVLRLLGLPEGPESSRDAVPSALSRWPAIEFETAAAADGL
                     AVAALRNPGQWEAHPQSAAVATLPLVEISRLDVDAGPLDWKDLPRTARPLEGLRVLEA
                     TRILAGPVAGRTLAAYGADVMLVNAPHLPNIDALADTSRGKRSALADLRSSDDRERFV
                     RALSGSHVFLHSYRRGAFEGLGFGADEIARLRPGIVHASLSAYGRLGPWATRRGFDSL
                     VQTASGFNVDEAAAAGVVEPKPLPVQMLDMASGFLLAFGVEAALLRQRAEGGSWQVQV
                     SLARTGQ"
     gene            1274..>4758
                     /gene="hrpA"
                     /locus_tag="VFF72_03930"
     CDS             1274..>4758
                     /gene="hrpA"
                     /locus_tag="VFF72_03930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HZV92340.1"
                     /translation="MRPLNARDGRPRPSTPPAPLAAIEFPEALPVSARRLEIAEAMRM
                     HQAIIVCGETGSGKTTQLPKIALSIGRGRANGGGLIGHTQPRRLAATSVARRIADELK
                     TPLGDVVGYKIRFQDRLSAGASVKLMTDGILLVETQGDPLLRAYDTIIVDEAHERSLN
                     IDFLLGHLRQIMSRRPDLKIVVTSATIDAERFAAHFASAAGPAPVLQVSGRLYPVEIR
                     WRPFVETRDHDLADAVCDAVDELWRDGPATRAALQTEQGDALPVPRVASGAGDVLVFL
                     AGEREIRDVAERLRKHHPPGVEVLPLFARLNQQQQDEVFAPHGARRIVLATNIAETSL
                     TVPGIRYVIDAGTARIKRYSFRSKVEQLPVEPISQAAASQRAGRCGRVADGICIRLYD
                     EADFAGRPRFTDPEIVRSSLAGVILRMKSLDLGSVEEFPFLEAPPRRAIADGYQLLSE
                     LGATDEQNELTPIGRELSRLPLDPRVGRMILAARERGALGDVLVVASALSTQDVRDRP
                     VEQQQAADQAHRKFDDERSEFTGDLKLWAWLEASRGGHGEHRLSARKHGQLLRDNFIS
                     PRRVREWRDVHSQLQTVVAEHGWRIDDAPASYDQLHLSMLAGLLGNVGVKSDDDDSYL
                     GARGIRFHRHPGAHLSKKPGRWIVAAELVETTRLYGRGIAGIDAKWLELVGGHLLKKQ
                     LLEPHWEKKAAQVVALERATLYGLVVYANRRVDFGRVDPVAAREIFIREALVNGEWET
                     KLPFLAANRKLIAQVEELEHKARRQDVLVDDELIHAFYDQQVPADVHDARSFERWYRD
                     EATRQPRLLVLSREELMRHEAAGITTQAFPKTVRLGGIDCAAEYRHEPGQSRDGITVT
                     VPVYALNATSEERCEWLVPGMLREKVAALAKTLPQRSRSRLVPLPGFADAFVEAHAFG
                     EGDLLDTLCADIAQRTGIAVRRGDFRQEQLAAHLSMNLRVVDEHGRQLGESRHLAELK
                     AAWGEKARSAFQALAALRLPQAAQASIGGSIESRASSASGNSSSVARSVPSAPSSSLH
                     RAPQAAAAAKGGSSTGAGAAPAAPAFASGRSAATAPAQTAAPQPEPARFTDWTFGDLP
                     ELMEVSQGGQSLIGYPALIDKKSHVEIEVFDEPESATARHRLGLRRLFALQLRETIRH
                     LEKNIPELQKMSVAF"
BASE COUNT          762 a         1659 c         1645 g          692 t
ORIGIN      
        1 cactgtcccg tcctcgccaa cgacacttgg acctgccagc tgcccccttc ggcgcgctgg
       61 cgcagcaacg ccgcttcgac gccgaaggcg agaagaaacc ccgaggccat gtcgagcatc
      121 tgcaccggca gcggcttcgg ctcgaccacg ccggctgccg ctgcctcgtc gacgttgaag
      181 ccgctcgcgg tctgcacgag cgagtcgaag ccacgccgcg tcgcccacgg cccgagccgt
      241 ccgtaggccg agagcgaggc gtggacgatg cccggtcgga ggcgagcgat ctcgtcggcg
      301 ccgaagccga gtccctcgaa cgcgccgcgt cgatacgaat ggagaaagac gtgcgaaccg
      361 gacagggcgc gaacgaagcg ttcgcggtcg tccgagcttc gcaggtcggc cagcgccgag
      421 cgcttgcccc ggctcgtgtc ggcgagggcg tcgatgttcg gcagatgcgg cgcgttgacc
      481 agcatcacat cggcgccata cgccgccagc gttcgcccag cgaccggccc ggcgaggatg
      541 cgcgtcgctt cgaggacgcg caggccctcg agcggccgcg ccgttcgcgg caggtccttc
      601 caatcgagcg gcccggcgtc gacgtcgagg cggctgatct cgacgagtgg aagcgtcgcg
      661 acagcagcgc tttgcggatg ggcttcccat tgccccggat tgcgaagggc ggcgaccgcg
      721 agtccatcgg ccgcggccgc ggtctcgaat tcgatcgccg gccagcggga gagcgccgag
      781 ggaaccgcgt cgcgactcga ttccggccct tccggcaggc caagaaggcg caggacgccg
      841 tcgcggtgat gggcgaaatt ggtgtgcacg cgaacccagc cgtcggcgca tggatagagc
      901 ccggcgatcg gatcccacac gttcggggcg ttgccgtcga cgaggaatgc ggccctcgcc
      961 tcgacggcgg cgtcgaccat gtccaccgag acccgttgta cgaggccgtt gcgcagtcgt
     1021 cccacttcgc tggccgcgag cgcggcggcg gcgagcgacg cctgcgccgc ggcgccgatg
     1081 gcgaacgagg agggaaggac cggatcggcg cccgtcaggt cgacgcgctc gagcgcggca
     1141 tccggcaaca gcgccggctt gcagacttcg cgcagcacat cgagggcggc agtggccatg
     1201 gcacgacgac tataggatgc cggccgatga cctgccgagc ccgacgaccc cggaaatcga
     1261 cgacgatcct gcgatgcggc cactgaacgc acgcgacggc cggcctcggc cgtcgacgcc
     1321 gccggcgccg ctcgcggcga tcgaatttcc cgaggcgttg ccggtcagcg cccggcgcct
     1381 ggaaattgcc gaggcgatgc gaatgcacca ggcgatcatc gtctgcggag aaaccggctc
     1441 cggcaagacg acgcagttgc cgaagatcgc gctttcgatc ggccgcggac gcgccaacgg
     1501 cggcggcctg atcggccaca cgcagccgcg acgtctcgcg gcgacaagcg tcgcgcgacg
     1561 catcgccgac gaattgaaga cgccgctcgg cgacgtcgtc ggatacaaga tccggttcca
     1621 ggatcggctc tcggccggtg ccagcgtcaa gctgatgacc gacggcattc tgctggtcga
     1681 gacgcagggc gacccgctgc tgcgcgccta cgacacgatc atcgtcgatg aagcccacga
     1741 acgaagcctg aacatcgact tcctgctcgg gcatctgcgc cagatcatgt cgcgccggcc
     1801 cgacctgaag atcgtcgtca cctcggcgac catcgatgcc gagcgcttcg ccgcgcactt
     1861 cgcgtcggcg gccggaccgg cgcccgtgct gcaggtgtcg gggcgcctct atccggtcga
     1921 gatccgctgg cggccgttcg tcgagactcg cgaccacgac ctcgccgacg ccgtctgcga
     1981 cgccgtcgac gagttgtggc gggacggccc tgcgacacgc gcggccctgc agactgaaca
     2041 aggcgatgcg ctgcccgtgc ctcgagtcgc cagcggcgcc ggcgacgtgc tggtgttcct
     2101 ggcgggcgag cgcgaaatcc gcgacgtcgc ggagcgcctg cgcaagcacc atccgccagg
     2161 ggtcgaggtg ctgcccctct tcgcgaggct caaccagcag cagcaggacg aggtcttcgc
     2221 gccgcacggc gcgcggcgca tcgtccttgc gaccaacatc gccgagacgt cgttgacggt
     2281 gcccggcatc cgctacgtca tcgatgccgg caccgcgcgc atcaagcgct acagctttcg
     2341 cagcaaggtc gagcagctgc cggtcgagcc catcagccag gcggcggcga gccagcgcgc
     2401 cggccgctgc ggccgggtcg ccgacggcat ctgcatccgc ctttacgacg aagccgactt
     2461 cgccggccgg ccgcgcttca ccgatcccga gatcgtccgc tcgtcgctgg ccggcgtcat
     2521 cctgcgcatg aagtcgctcg atctcggcag cgtcgaggaa tttccgtttc tcgaggcgcc
     2581 gccgcgcagg gcgatcgcgg acggctacca actgctctcc gagctcggcg cgaccgacga
     2641 gcagaacgag ctgacgccga tcggccgtga actgtcgcga ctgccgctgg atccgcgcgt
     2701 cggccggatg atcctggcgg cgcgcgagcg gggcgcgctg ggcgacgtcc tcgtcgtcgc
     2761 cagcgcgctc tcgacgcagg acgtgcgcga ccggccggtc gagcagcagc aggcggccga
     2821 ccaggcgcac cgcaagttcg acgacgagcg ttccgaattc accggcgact tgaagctctg
     2881 ggcgtggctc gaggcttcgc gcggtggtca tggcgagcat cgcctctcgg cccgcaagca
     2941 cgggcagctg ctgcgcgaca acttcatctc gccccggcgc gtgcgcgagt ggcgcgacgt
     3001 gcactcgcaa ttgcagacgg tcgtcgccga gcacggctgg cgcatcgacg acgcgccggc
     3061 gagctacgac cagctccact tgtcgatgct tgccggactg ctcggcaacg tcggcgtgaa
     3121 gagcgacgac gacgattcgt acctcggcgc gcgcggcatc cgctttcatc gtcatcccgg
     3181 cgcccacctc tcgaagaagc ccggccgctg gatcgtcgcc gccgagctcg tcgaaacgac
     3241 gcggctctac ggtcgcggca tcgccggcat cgacgcgaag tggctcgaac tggtcggcgg
     3301 ccatctgctg aagaagcagc ttctcgagcc gcattgggaa aagaaggccg cccaggtcgt
     3361 ggcgctggag cgggcgacct tgtacggtct cgtcgtctat gcgaaccggc gcgtcgattt
     3421 cggccgcgtc gacccggtgg ccgcgcgcga gatcttcatt cgcgaagcgc tcgtcaacgg
     3481 cgaatgggag acgaagctgc cgttcctggc cgccaatcgc aagctgatcg cgcaggtcga
     3541 ggagctcgag cacaaggccc ggcgccagga cgtgctcgtc gacgacgagc tcatccatgc
     3601 cttctacgat cagcaggttc ccgccgatgt gcacgatgca cgcagcttcg agcgctggta
     3661 tcgcgacgag gcgacgaggc agccccggct cctggtcctg tcgcgggaag agctgatgcg
     3721 ccacgaggcc gccggcatca cgacgcaggc ctttccgaag acggtgcggc tcggcggcat
     3781 cgactgcgcc gccgagtatc gacacgagcc cggccagagt cgggacggga tcacggtgac
     3841 ggtgccggtc tacgccctca acgcgaccag cgaggagcgc tgcgaatggc tcgtgcccgg
     3901 gatgctcagg gagaaggtcg ccgctttggc gaagacgctg ccgcagcgct cgcgatcgcg
     3961 cctcgtcccg ttgcctggtt tcgccgatgc cttcgtcgaa gcgcacgcgt tcggcgaggg
     4021 ggatcttctc gacacgctgt gtgccgatat cgcgcagcgc accggcatcg ccgtcaggcg
     4081 cggcgacttt cgccaggagc agcttgccgc ccatctgtcg atgaatctgc gcgtcgtcga
     4141 cgagcatgga cgccagctcg gtgagtcgcg ccatcttgcc gagctgaagg cggcctgggg
     4201 cgaaaaggcg cgctcggcgt tccaggcgct ggcggcgctg cgcttgccac aggcagcaca
     4261 agcgtcgatc ggggggtcca tcgagtcccg cgcatcgagt gcaagcggca attcgagctc
     4321 cgtggcgaga tcggtgccgt ctgccccgtc gtcgtcgctt catcgtgcgc cgcaggccgc
     4381 agccgctgcg aaaggcggat cgtcgacggg tgcgggggct gcgccggctg ccccggcgtt
     4441 cgcgagcggc cggtcagccg caacggcgcc tgcccagacc gccgcgccgc agcccgaacc
     4501 ggcgcgcttc accgactgga ccttcggcga tcttccggag ttgatggaag tgagccaggg
     4561 cggccagtcg ctgatcggct accccgcgct catcgacaag aagagtcacg tcgagatcga
     4621 ggtcttcgac gagcccgaat ccgccactgc cagacatcgg ctgggcctgc gcaggctctt
     4681 tgccctgcag ctgcgcgaga cgatccgcca tctcgaaaag aatatcccgg aactgcagaa
     4741 gatgtcggtg gccttcat
//
LOCUS       DASRWS010000211         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3255247, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000211 DASRWS010000000
VERSION     DASRWS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255247"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFF72_03935"
     CDS             <1..265
                     /locus_tag="VFF72_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZV92341.1"
                     /translation="GQVWQGVGLVVFSVVVIGLIDNILRPILVGKNTQLPDYLILLST
                     IGGLAVIGLNGFVIGPVIAAMFVSAWQLFASERRRRPSVDAST"
     gene            400..621
                     /locus_tag="VFF72_03940"
     CDS             400..621
                     /locus_tag="VFF72_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92342.1"
                     /translation="MDEPLDRIRELEARLVAHGLILKTIARLLSESTLAELKAAAQGI
                     DDAGMQEANPDLHMRRVAEEMFGILEMVG"
     gene            complement(694..1197)
                     /locus_tag="VFF72_03945"
     CDS             complement(694..1197)
                     /locus_tag="VFF72_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HZV92343.1"
                     /translation="MSTHTSAGVAALVARLGGSLRGKGATMAAAESCTGGLIAAACTS
                     LAGSSDWFERGFVTYSNAAKIELLSVPDELIARHGAVSEEVARAMAEGALAHSRARFA
                     VAATGIAGPGGGTLLKPVGTVWIAVASTGSAAHASLLRLHGERDSIRQASVIEALSLL
                     AEHVDRS"
     gene            complement(1194..1751)
                     /locus_tag="VFF72_03950"
     CDS             complement(1194..1751)
                     /locus_tag="VFF72_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZV92344.1"
                     /translation="MSGEPAVNPPLRGGARRPGWRFLVSHPAHLLALGFGSGLSPVAP
                     GTAGTLFAWLVWEVLPESAIGAAGVAVALAVCTLVGWWACTVTARHLGQADPSAVVCD
                     EIVAFWVVLWLVMPAGFVAQGVAFVLFRLFDAVKPGPVAWADQLFKSRRGETPGWAQG
                     FGIMFDDFVAALCSLLVIALWRFVA"
     gene            complement(1748..>2025)
                     /locus_tag="VFF72_03955"
     CDS             complement(1748..>2025)
                     /locus_tag="VFF72_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZV92345.1"
                     /translation="LPRSSDVAALTPGWQRTCLLCGGDDYELVFTAPPGLAAAVADAG
                     VASSTPVTRIGSIVEGRGLRFVDATGCEVKEPAASFDHFRESAEPPP"
BASE COUNT          355 a          720 c          672 g          278 t
ORIGIN      
        1 cggccaggtc tggcaaggcg tcggactggt cgtcttcagc gtcgtcgtca tcggcctcat
       61 cgacaacatc ctgcggccga tcctcgtcgg caagaacacc cagctgcccg actatctgat
      121 cctcctgtcg accatcggcg gcctcgcggt catcggcctc aacggcttcg tcatcggccc
      181 ggtgatcgcc gcgatgttcg tttcggcctg gcagcttttc gccagcgagc gaaggcgtcg
      241 accgagcgtc gacgcctcca cctgaaggag ggaaggtcac gccgagtcgg cgccgtccta
      301 cgcgcaaacg gtcctcgctc tgtcaggatt gatcgcccgc ccgcgctcgc gcgacccgag
      361 ttcaacccgt aagctcgccg acttccgaag aacaatctca tggacgaacc cttggaccgc
      421 atccgtgaac tcgaggcgcg cctcgttgcc catggcctca tcctgaagac gattgccagg
      481 ctgctttccg agtcgacgct cgccgagctg aaggcggcag cccagggcat cgacgacgcc
      541 gggatgcagg aagccaatcc cgacctgcac atgcggcgtg tcgccgagga gatgttcggc
      601 attctcgaga tggtcggcta ggcgcccatc tcgagacgct gccggcccgc ggccgcccgc
      661 accgccttca gcgacggcgc tgacctgcgc cgatcaggac cggtcgacgt gttccgccag
      721 gagcgaaagc gcttcgatca ctgacgcttg gcgaatcgag tcacgctcgc cgtgcaatct
      781 cagcaagctg gcatgagccg cgctgcctgt cgatgcaacc gcgatccaca ccgtgccgac
      841 gggcttgagc aaggtgccgc cgccggggcc ggcaatgccc gtcgccgcga ctgcgaagcg
      901 ggctctcgaa tgcgccagcg ccccttcggc catcgcgcgt gccacttcct cgctgacggc
      961 accatgcctg gcgatcagct cgtcggggac ggaaaggagc tcgatcttgg ccgcattcga
     1021 ataggtgacg aaaccgcgct cgaaccagtc gctcgacccg gccagcgagg tgcatgccgc
     1081 agcgatcagg ccgccggtgc aggattcggc ggcggccatg gtcgcgccct tgccgcgcag
     1141 cgacccgccc aagcgcgcca cgagggcggc aacgccggcc gacgtgtggg tgctcatgcg
     1201 acgaagcgcc agagcgcgat gacgaggagg ctgcacagcg cggcgacgaa gtcgtcgaac
     1261 atgatgccga agccctgcgc ccagcccggt gtttcgccgc gccgggattt gaagagttga
     1321 tcggcccagg cgaccggacc gggcttgacg gcatcgaaga ggcgaaagag gacgaatgcg
     1381 accccctgcg cgacgaaccc cgccggcatg acgagccaga gaacgaccca gaaggcgacg
     1441 atctcgtcgc acaccacggc cgacgggtcg gcctggccga gatgtcgcgc ggtcacggtg
     1501 caggcccacc agccgacgag cgtgcagacg gcgagggcaa cggcgacacc ggctgcaccg
     1561 atcgccgatt ccgggagcac ctcccagacg agccatgcga agagagtgcc ggcggtcccg
     1621 ggcgcgaccg gggacaggcc gctgccgaat cccagcgcca acaggtgcgc cggatgactg
     1681 accagaaagc gccagccggg ccgcctcgcg ccgccgcgaa gcggcggatt gacggccggt
     1741 tcgccgctca tggcggcggc tccgcagact cgcggaaatg atcgaacgac gcagccggct
     1801 ccttcacttc gcaccctgtc gcatcgacga agcgcaggcc tcggccctcg acgatgctcc
     1861 cgatccgcgt caccggcgtc gacgatgcga cgccggcgtc ggccaccgcg gcggcaagcc
     1921 ctgggggcgc ggtgaacacc agctcgtagt cgtcgccacc gcaaagaagg caggtgcgct
     1981 gccagccggg cgtcagcgcc gcgacatcgc tgctgcgcgg cagcg
//
LOCUS       DASRWS010000212         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2663210, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000212 DASRWS010000000
VERSION     DASRWS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663210"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..491
                     /locus_tag="VFF72_03960"
     CDS             138..491
                     /locus_tag="VFF72_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92346.1"
                     /translation="MLPERLTRVSPALSRPIVATLVVAFVWLGYGGWSSWSGAHKLEA
                     AGIGAGAGRINVEIVLAFPPESFHLTRIQDIGRLTGVRGRSVFLKDVTGADLRALARN
                     YWVREIHRAPGSGAG"
     gene            521..1333
                     /locus_tag="VFF72_03965"
     CDS             521..1333
                     /locus_tag="VFF72_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92347.1"
                     /translation="MSPQSVAWRLGRRGARIAKGALGLVLFFVAWQVSVPLVGLEPYF
                     YPSPIDVWHAFVDLLAKGILPVYLADSVGRYLAGVGLGTVIGVAAGLGIGLVRPLARM
                     LSPLINFLFAIVEVAWIPIFVIWWGYGLTTILVALVYVVFFPLLYNTLLGVRTVPQVY
                     VNAVRSLGASRLQVLTQVILPAALPNIIAGFRVGAGFAFRGLIFAEIVAAKSGIGYLI
                     FEAAAHHQTARTIVGMIVMGLLWLFLDNFYLKPFERATVERWGQTTTAERRE"
     gene            1330..2118
                     /locus_tag="VFF72_03970"
     CDS             1330..2118
                     /locus_tag="VFF72_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92348.1"
                     /translation="MSGAVLRRSTLADRATGLVPFVAIILAWVIAPYLADYPSYVLPS
                     IGQVAERTWTGLSDGSLLEHVLGSLYRLAIGFVVGNAIAIPLGIAIASNRHVADLLRP
                     LLTFLQSIAGIAWVPLAIIWFGIGMGAVVFVIANTIFFSAIYNTVVGVESIPNILYRA
                     VRSQGARGWQVFTELVLPGALVQIILGLRTSMAYGWRALVAGEMIAGTNGLGYMTVEA
                     VQWYQTDTIIMGMIVIGLLWLILDRLLFVSLERATVARWGLIQR"
     gene            2115..>2540
                     /locus_tag="VFF72_03975"
     CDS             2115..>2540
                     /locus_tag="VFF72_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV92349.1"
                     /translation="MRSDAIALPRLGLGTAAALGVMGTAISDEAADAVVATAYARGIR
                     FFDTAPLYGGGLAEERLGRVLRTLPRDDIVLSTKTGVTRPYRQAASPPGGLRRRSADV
                     WDYSPEATKRSVALSLERLGVERLDLVHLHDVDGRVDQAM"
BASE COUNT          344 a          863 c          824 g          509 t
ORIGIN      
        1 cgccggcccc ggccgggtca aggagatcat cgcggtgccg atcgcacgcg agcggcggaa
       61 ttggcagacg ctcaatgccg acccgcagtt cattgcgctg cgcgaccagg tgctcggcct
      121 cgtgcgcgag acgaggcatg ctgcctgaac ggttgacccg ggtaagcccg gcgctgtccc
      181 gcccgatcgt cgcgacgctc gtggttgcat tcgtatggct gggctacggc ggatggtcga
      241 gctggtccgg cgcacacaag cttgaagctg ccggcatcgg cgccggtgcc gggcgcatca
      301 acgtcgagat cgtcctcgcg tttccgccgg aatccttcca tctcacgagg atacaggata
      361 tcgggcggct gaccggggtc cggggccgat cggtcttcct caaggacgtg acaggcgccg
      421 acctccgcgc gctcgcgcgc aactattggg tcagggaaat ccatcgtgct ccgggaagcg
      481 gcgcagggtg atgggacagg cgcgacccga tgcacccgcg ttgtcgccgc aatcggtcgc
      541 ctggcgcctc ggccggcgcg gcgccaggat tgccaagggc gcgctcggcc tcgtgctgtt
      601 ttttgtcgcc tggcaggtct cggtgccgtt ggtcgggctc gagccctatt tctacccgtc
      661 gccgatcgat gtgtggcatg ccttcgtcga cctgctcgcc aagggcatcc tgcccgtcta
      721 cctcgccgat agcgtcggcc gctatcttgc cggggttggg ctcgggaccg tgatcggtgt
      781 cgccgccgga ctcggcatcg gcctcgtccg tccgctcgcg cgcatgctct cgccgctcat
      841 caattttctc ttcgccatcg tcgaggtggc gtggatcccg atcttcgtga tctggtgggg
      901 ctacggcctc acgacgatcc tcgtcgccct ggtctacgtc gtgttcttcc cgcttctcta
      961 caacacgctg ctcggcgtgc gcactgtccc gcaggtctac gtcaatgccg tgcgatcgct
     1021 cggcgcgagc cgcctgcagg tcctcaccca agtcatcctt ccggcggcgt tgccgaacat
     1081 catcgctggg ttccgcgttg gcgccggctt cgccttccgc ggcctcatct tcgccgagat
     1141 cgttgcggcg aagagcggca ttggctacct catcttcgag gccgccgcgc atcaccagac
     1201 cgctcgtacg atcgtcggca tgatcgtgat ggggctgctc tggcttttcc ttgacaattt
     1261 ctacctgaag cccttcgagc gagcgaccgt cgagcgctgg ggtcagacga caacggcgga
     1321 gcgtcgcgaa tgagtggcgc ggtcttgcgc cggtcgacgc tggcggaccg cgcgacgggt
     1381 ctcgttccct ttgtcgcgat cattctcgcc tgggtgattg cgccctacct cgccgactac
     1441 ccaagctacg tgctgccgtc gatcgggcag gtcgccgagc ggacctggac ggggctgagc
     1501 gacggctcgc tgctcgagca tgtgctcggc agcctctatc gcctggcgat cggcttcgtc
     1561 gtcggcaatg cgatcgccat cccgctcggc atcgccatcg cctccaatcg ccatgtcgcc
     1621 gatctgctcc gcccgctgct caccttcctg cagtcgattg ccggcatcgc ctgggtgccg
     1681 ctggccatca tctggttcgg catcggtatg ggtgccgtcg tcttcgtgat cgccaacacg
     1741 attttcttca gcgcgatcta caatactgtg gtcggcgtcg agagcatccc gaacatcctc
     1801 taccgagcgg tgcgcagcca aggggcgcgc ggctggcagg tcttcaccga gctcgtcctg
     1861 ccgggcgccc tcgtacagat cattctcggc ttgcgcacct cgatggccta tggctggcgc
     1921 gcccttgtcg ccggcgagat gatcgctgga accaacggtc tcggctacat gacggtggag
     1981 gccgtgcagt ggtatcagac cgacacgatc atcatgggaa tgatcgtcat cggtctcctg
     2041 tggctcattc tcgaccgcct gctcttcgtt tcgctcgagc gggcgacggt cgcgcgttgg
     2101 ggcttgatcc aacgatgaga agcgatgcca tcgcgttgcc gcgcctcggc ctcggcaccg
     2161 ctgcggcgct tggggtcatg gggacggcga tcagcgacga ggcggccgac gccgttgtcg
     2221 cgacggccta cgcgcgcggc atccgcttct tcgacacggc accgctctat ggcggtgggc
     2281 tcgccgagga gcgcctcggc cgcgtacttc ggacattgcc gcgcgacgat atcgtcctct
     2341 cgaccaagac cggggtgacg cggccatacc gtcaggcggc gtcgccgcct ggcggcctgc
     2401 gccggcgctc tgccgacgtc tgggactata gtcccgaggc cacaaagcgc tcggtcgcgc
     2461 tcagtctcga gaggctcggc gtcgaacggc tcgaccttgt tcacctgcac gatgtcgacg
     2521 gacgcgtcga tcaggccatg
//
LOCUS       DASRWS010000213         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1522996, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000213 DASRWS010000000
VERSION     DASRWS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522996"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..459
                     /locus_tag="VFF72_03980"
                     /pseudo
     CDS             1..459
                     /locus_tag="VFF72_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
     gene            660..1871
                     /locus_tag="VFF72_03985"
     CDS             660..1871
                     /locus_tag="VFF72_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZV92350.1"
                     /translation="MRWARRLSGRFAGLERLRTGAKGQRRVAWLTERLALVRSSQGIV
                     AITVAPVWQLAALSFGGVSLTALIAGIAVAGVFQGGNERQAQENERQAREIVALRGEM
                     QALAERTTQAEHERTVALIEAGHRAVTERDTMVAETMTGQSRLAAERDAAVERADSTL
                     EQANLDRRQLEQERDRARTERDDLANEVDVALRELDSETRHTLADIEKIFAATGVDPR
                     LARPSSNRGEARGGPYIPWQGFAAASAESTNRFEGVARRLERLKALRDALQQLPIVMP
                     VTRAVLSGGFGFRFDPFNGVSARHEGVDLRATFDSTVYAPGVGTVISAGWSGEFGNMV
                     EVDHGFGVVTRYAHLSRISVRVGDVLTSRQPIGVIGATGRATGAHLHFEIRVAGRAID
                     PLKFLHAAEVR"
     gene            complement(1901..>2250)
                     /locus_tag="VFF72_03990"
     CDS             complement(1901..>2250)
                     /locus_tag="VFF72_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HZV92351.1"
                     /translation="PTGGLNSAHTHPNNYLSGVYYVSTPTGADAIEFTDPRPAAVACM
                     PRVKQLNRFNGNRMTVQAKPGRFVLFPAWLSHNVPVNRTNHERVSIAFNAMFANFAET
                     MSSPLWKGTLHLK"
BASE COUNT          346 a          722 c          832 g          350 t
ORIGIN      
        1 tcctgcctac ctgccgcagg gctcggagct gttcgacgat taccggacgg cggtcgaatg
       61 ggcgcagcag tacgcggcgc tcaaccggcg caccatgatg atggcggcac tcgaggcctt
      121 gacggccgag gtggccaagc cgttcgagct ggccgagcct gcggcatcat cccgggcagc
      181 atgggcgcgc ggtcgttcat cgtccgcggc aagggcaacg cccaaccgtt ccactcgtgc
      241 agccacgggg cgggacgggc gatgtcgcgg gccgaggcca agcggcggtt cgccgtcgag
      301 gaccacgtca aggcgaccgc gggcgtcgag tgccggaagg atgccgaggt gatcgacaag
      361 acgcctggcg cctacaagga tatcgacgcc gtcatggcgg cgcagtccga cctcgttgag
      421 gtggtgcaca cgctccggca ggtgctctgc gtcaagggcc gagaaacgag ggcggaggag
      481 ccgaaggctt cccccgcctt tttgcgccgc cactctgtcg ccggccacaa gagtcgcagc
      541 ctgccccaga ggtaacggcc cgacgggacg ttcacgtttt cgcgtgcgtc aaatcaacgg
      601 atgcgccgtc ggccgatcgt gcgtattgtc cggattgggg cagttgggaa tttcatttca
      661 tgcgctgggc acgacggcta tcaggccgct tcgcggggct cgagcgtttg cgcaccgggg
      721 caaaggggca gcggcgcgta gcatggctca ccgagcggct ggcactggtc cgcagttcgc
      781 agggaatcgt cgcgatcacg gtcgcgccgg tgtggcagct cgcggcgctt tccttcggcg
      841 gtgtatcgct tacggcgctg atcgccggca ttgcggtggc cggggtcttc cagggcggca
      901 acgagcggca ggcgcaggaa aacgagcggc aggcccggga aatcgtcgcg ctgcgcggtg
      961 agatgcaggc tctggccgaa cggaccacgc aggcggagca cgaacgcacc gtggcgctga
     1021 tcgaagccgg ccatcgcgcg gtcaccgagc gcgacacgat ggtcgcggag acgatgaccg
     1081 ggcagtcgcg actggccgcc gagcgcgatg ccgcggtcga gcgcgccgac tcgacgctcg
     1141 agcaggccaa cctcgatcgc cggcagctcg agcaggagcg cgaccgggcc aggaccgagc
     1201 gcgacgatct cgcgaacgag gtcgacgtcg ccctgcgcga gctcgacagc gagacgcggc
     1261 acacgctggc cgacatcgag aagatcttcg cggccaccgg cgtcgaccct cgcctggcgc
     1321 gtccgtcgtc gaatcggggc gaggcccgtg gcggtcccta cattccctgg cagggctttg
     1381 cggctgcaag cgcggagagc accaaccgat tcgagggcgt ggcgcgtcgc ctcgagcggc
     1441 tgaaggcgct gcgcgatgcc ctgcagcagt tgccgatcgt catgccggtg acccgcgcgg
     1501 tgctgtcggg cggcttcggc ttccggttcg atcccttcaa cggcgtgagc gcgcggcacg
     1561 agggcgtcga tctgcgggcg accttcgatt cgaccgtcta tgcgcccggc gtcggcacgg
     1621 tgatctcggc cggctggagc ggcgagttcg gcaacatggt cgaggtcgac cacggcttcg
     1681 gcgtggtgac gcgctacgcc catctctcgc gcatcagcgt gcgcgtcggc gacgtgctga
     1741 cgtcgcgcca gccgatcggc gtgatcggcg ccaccggccg ggcgaccggc gcgcatctgc
     1801 acttcgagat ccgcgtcgcc ggccgggcga tcgacccgct caagttcctc cacgccgccg
     1861 aggtccgcta gcctggcgcg gggtcgacgt ggaccggcgg tcacttcagg tgaagggtgc
     1921 ccttccacaa gggtgagctc atcgtctcgg cgaagttcgc gaacatggcg ttgaaggcga
     1981 tgctcacgcg ctcgtggttg gtgcgattga ccgggacgtt gtgcgacagc cacgccggga
     2041 acagcacgaa gcggcccggc ttggcctgca cggtcatgcg gttgccgttg aaccggttga
     2101 gttgcttgac gcgcggcatg caggcgacgg ccgccgggcg ggggtcggtg aactcgatgg
     2161 catcggcgcc ggtcggcgtg ctcacgtagt agacgccgct gagatagttg ttgggatggg
     2221 tatgggcgct gttcaaaccg ccggtcggat
//
LOCUS       DASRWS010000214         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_661957, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000214 DASRWS010000000
VERSION     DASRWS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661957"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFF72_03995"
     CDS             complement(<1..609)
                     /locus_tag="VFF72_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92352.1"
                     /translation="MKLVGLRSDPDEQLREINHFLGNQLEGSAPEARYARAAAFPPAD
                     DVLPPKVKETVARPPAAASDVRDVGCADLGRILSDDQVAAMHEHLRAKPVLLGHIPHS
                     ARGHAASLDLVPSESNYACYSYLDLWSSPGLIQFASQAKILDLAQAYLGCTPTLYSIN
                     AFWSLPNRASHKATQVFHRDWEDYRSLALFTQLTPVEKPEDGA"
     gene            complement(854..1555)
                     /locus_tag="VFF72_04000"
     CDS             complement(854..1555)
                     /locus_tag="VFF72_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92353.1"
                     /translation="MYRIDRHDIVAEDEAIWQLDDDALLAELQRMPSTPQAWIRQSDH
                     WGLRNLHTNACGDFTLMALRHWHRLRGHPHDDTVLTLDIDSLVMHAAAALGVRECRWP
                     DDCRIYKPRHENLSNARISQVWSSWQRALDKVLSQRVGREAALGARIFFDYPRRKIRG
                     VESVIGPSIERNFVAPASRWALGSKPPAWQPENWGLADVTLEERVICQADWETIPLAR
                     RKVPPKVKLSADHHD"
     gene            1535..2101
                     /locus_tag="VFF72_04005"
     CDS             1535..2101
                     /locus_tag="VFF72_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92354.1"
                     /translation="MAIDPVHRALVEVATGQRGDGLRREEGVGRLGRDEPAARATEAD
                     IGRLAGMDGALGGAGEGEVVLRFDDDTANADVPQMRWHIEDVDEVRAAGRRIPVGDDD
                     LAVDAGAFELPRKAGHGAVDPADVARRIGVIAAIGDEADVRGRGHSAVGRSGGLVWLH
                     ELPGMTFQEAVRRGPLHIFRTHFAKASR"
     gene            2098..>2283
                     /locus_tag="VFF72_04010"
     CDS             2098..>2283
                     /locus_tag="VFF72_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92355.1"
                     /translation="MTFVIPRQIRLETNIVLQSAKRQCARRLQCERSFRISVAQRSLA
                     GPVRVNHLKSPLDVEPQP"
BASE COUNT          413 a          680 c          778 g          412 t
ORIGIN      
        1 agcgccgtcc tccggcttct cgaccggcgt gagctgagtg aacagcgcca gagagcggta
       61 gtcctcccag tcacgatgga agacctgagt ggctttgtgc gatgcccggt tgggcagcga
      121 ccagaaggcg ttgatcgagt acagggtcgg cgtgcagccc aagtaggctt gcgccagatc
      181 caggatcttg gcctgcgacg cgaactggat gaggcccggg ctcgaccaga ggtcgagata
      241 gctgtagcag gcgtaattgc tctcggatgg gactagatcg agagacgctg catggccgcg
      301 cgcagaatgc ggaatgtgtc ccagcaggac gggctttgcg cgcaagtgct catgcatcgc
      361 cgcgacctga tcgtcgctca ggatccggcc caggtcggcg caccccacgt cgcgcacgtc
      421 actcgcagcg gccggcggtc gggccactgt ctccttcacc ttgggtggca gcacgtcgtc
      481 cgctggagga aaggcggccg cgcgcgcata gcgggcctca ggcgcactgc cttccagctg
      541 gttgccgagg aaatgattga tctcgcggag ctgctcgtcc ggatcggacc gaagaccaac
      601 aagcttcaac gcaagtcggg tgtcgccaat gcgagcgacc accttcttca ggcgggcgag
      661 atcaagccgt agcgcctcgg aaagcgcagt ctgggctccc cctgcactcc aaagccgttg
      721 ccaaatagac atatttccca acgtccgacg cgtcgggcgc gttgtagaag taccttgacc
      781 aagacgcaac ggttgccttt gggcggcgtg ggaaaactgc aggggcagtc tccgagatcg
      841 gcgctagagg ccttcaatcg tgatgatcgg ctgaaagttt gactttcggc ggcacctttc
      901 tccgtgcgag tgggatggtt tcccagtctg cttggcagat cacgcgctcc tcgagtgtca
      961 catcggccaa cccccaattc tccggctgcc atgctggtgg ctttgatccc agcgcccagc
     1021 ggctggcggg cgccacgaag ttgcgctcga tcgaggggcc gatcacggat tcgacgccgc
     1081 gaatcttgcg ccggggatag tcgaagaaga tgcgcgcgcc gagggctgct tcgcggccga
     1141 cccgctggga aaggaccttg tcgagcgcac gctgccagct gctccacacc tgcgagattc
     1201 gcgcattgct gaggttttcg tggcgtggct tatagatacg gcagtcgtcc ggccagcggc
     1261 attcgcgcac gccgagcgcg gcagcggcgt gcatgacgag cgaatcgatg tcgagggtaa
     1321 gcactgtgtc atcgtgcgga tgaccgcgca ggcgatgcca gtggcgcagc gccatcaagg
     1381 tgaagtcgcc gcaggcgttg gtgtgcaaat tgcgaaggcc ccagtgatcg gattgccgaa
     1441 tccaagcctg cggcgtcgac ggcatccgct gcaactcggc aagtagggcg tcgtcgtcga
     1501 gctgccagat cgcctcgtcc tcggccacga tgtcgtggcg atcgatccgg tacatcgtgc
     1561 cctcgtcgag gtcgcgacgg gccagcgtgg cgatggcctg cggcgagaag aaggtgtcgg
     1621 acgccttggg cgtgacgaac cggccgcgcg cgcgacggag gccgacattg gccgcctcgc
     1681 cggcatggat ggcgcgctcg gcggcgccgg cgaaggcgag gtggtgctca ggttcgacga
     1741 cgataccgcg aatgctgacg tgccgcagat gcgatggcac atcgaggatg tcgatgaggt
     1801 gcgggcggcc ggccggcgga ttccagtcgg cgatgatgat ctcgccgtcg atgccggcgc
     1861 tttcgagctg ccgcgcaagg caggccatgg cgcggttgac ccggcggacg tagctcggcg
     1921 tataggcgtc attgcggcca taggtgacga agctgacgta cggggtcgcg gtcattctgc
     1981 tgttggtcgg tcgggagggc tcgtctggct tcatgaactg ccggggatga catttcaaga
     2041 agcggtaagg cgtggcccgc tacatatatt tcgtacgcat ttcgccaaag cgagtcgatg
     2101 accttcgtca tcccccggca gatccgcctt gaaacgaaca tagtgctcca aagtgcaaaa
     2161 aggcagtgtg cacgccggtt gcaatgcgag aggagtttcc gtatttcagt agcacaacgg
     2221 tccctcgccg ggccggtgcg cgtcaaccac ttgaaaagcc ccctggatgt cgagccgcag
     2281 ccg
//
LOCUS       DASRWS010000215         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2101852, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000215 DASRWS010000000
VERSION     DASRWS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101852"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFF72_04015"
     CDS             <1..979
                     /locus_tag="VFF72_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HZV92356.1"
                     /translation="SYATYIKDRLSGFAGDHPRQIALDLQAFPEFRARMAVFAGKQSF
                     KRQACVGPIEIVDRQSLRNDLRNLRAAVQRHGAHDAFMNAASPGVVSAFQPNMYYPTH
                     AAYVEALATAMREEYEAIVAAGFVLQIDCPDLAMAHHTGFQDLSEAEFLKRAEHQVEA
                     LNEALRNVPATSLRMHLCWGNYEGPHTHDIALEKVLPIVLKAKPAAILFEAANPRHAH
                     EWTVWRSARVPQDKILVPGVLTSTSNYVEHPELVAQRLCQFADIVGRERIMAGTDCGF
                     GTFAGIGKMDAQISWLKLKSLVEGAERASQRLGAHPDAFRVRLRHARPP"
     gene            1013..1786
                     /locus_tag="VFF72_04020"
     CDS             1013..1786
                     /locus_tag="VFF72_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZV92357.1"
                     /translation="MATQPLILGSEVAKRIFRAGRSDSLASHVVAEVRDSLFAGRLAP
                     GDFLGTEKDIAARFGVSRIVARDALRSLEALGIAQIKMGKGGGARIAAGNPSLFAEAL
                     AVQLHLTGVSAAEIMDAQRAAECMAAELAAEHATAAEHARLEALLVDAAAKTTDTAAF
                     TLSCRDFHLAIAEASHNRVLVVQLASLQYVSWPKDNRQVTPKLVKHILDIHHELAGLI
                     QLRDGAAARRLMDEHVRMIRARRVARPERAAAQTGTGCC"
     gene            1823..>2083
                     /locus_tag="VFF72_04025"
     CDS             1823..>2083
                     /locus_tag="VFF72_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92358.1"
                     /translation="MSKFIIQPHFRLQEWVAEEKGYFAAEGLDYVFNEEVQATDGKVH
                     DKGKKVGAYQSFEAGRSSDVSCACHWTVNVAASNGHGKMMTDV"
BASE COUNT          378 a          689 c          660 g          356 t
ORIGIN      
        1 cagctacgcc acctacatca aggaccggct gagcggcttt gccggcgacc atccacggca
       61 gatcgccctc gacctgcagg cgtttcccga gtttcgggcg cgcatggccg tgttcgccgg
      121 caagcagtcc ttcaagcgac aggcctgcgt cggcccgatc gagatcgttg accgtcagtc
      181 gctgcggaac gacttgcgca acctgcgcgc ggccgtgcag agacatggcg cgcacgatgc
      241 attcatgaac gcggcctcgc caggcgtggt ctcggccttc cagccgaaca tgtactaccc
      301 gacgcacgca gcctacgtcg aagcgctggc aacggcgatg cgcgaggagt acgaggctat
      361 cgtcgccgca ggcttcgtgc tccagatcga ttgtccggat ctggcgatgg cgcatcacac
      421 gggtttccag gacctctccg aagccgagtt cctcaaacgc gctgagcatc aggtcgaagc
      481 tctcaacgag gcgctgcgca acgtgcccgc cacgtccctg cgaatgcatc tttgctgggg
      541 taactacgaa gggccgcata cgcacgatat cgcgctcgag aaagtgcttc ctatcgtgtt
      601 gaaggcgaaa ccggccgcga tcctcttcga ggccgccaat ccgcgccacg cgcacgaatg
      661 gaccgtgtgg cgctcagcca gggtcccgca ggacaagatc ctggtcccgg gtgtgctcac
      721 ttcgacctcg aactatgtcg agcaccccga gctggtggcc cagcgtctgt gccagttcgc
      781 ggacatcgtc ggccgcgagc gcatcatggc tggaacggac tgtggtttcg gcaccttcgc
      841 cggcatcggc aagatggacg cacagatctc gtggctgaag ctcaagtccc tggtcgaagg
      901 ggccgagcgc gcctcgcagc gcctcggggc ccaccctgac gcgttccggg tccggctgcg
      961 gcacgcgcgg ccgccttgag tacggctggc tttaaagata tcatctttaa ccatggccac
     1021 tcagccgctc atcctcggta gcgaggtcgc caaacggatt ttccgcgccg ggcgctccga
     1081 ctcccttgcc tcccacgtcg tggccgaggt gcgcgattcg ctgttcgccg gccggcttgc
     1141 gccgggggat ttcctgggca ccgagaaaga catcgccgcc aggtttggcg tgagccgcat
     1201 cgtggcacgc gacgcgctac gctcactgga agcgttgggc atcgcccaga tcaagatggg
     1261 caaaggcgga ggtgcgcgta tcgcggcggg caaccccagc ctgttcgctg aagcgctggc
     1321 ggttcagctg catctgacag gtgtcagcgc ggctgagatc atggatgcgc aacgcgcggc
     1381 agaatgcatg gcggcagaat tggcagctga gcacgcaacc gctgcggagc acgccagact
     1441 cgaggcgctg ctcgtcgacg cagccgcgaa gaccacggac actgccgcgt ttaccctctc
     1501 ctgccgcgac tttcacctgg cgatcgccga ggcgtcgcac aaccgcgtgc tcgtcgtaca
     1561 gctcgcctcc ctgcagtacg tatcgtggcc gaaggacaac cgccaggtca cgcccaagtt
     1621 ggtcaaacac atcctggaca tccaccacga gctcgccggg ctcattcagc tgcgcgatgg
     1681 ggcggctgca cgccgcctca tggacgagca cgtgaggatg atccgcgcgc ggcgcgttgc
     1741 ccgaccggag cgtgccgccg cacaaaccgg aacggggtgc tgctgaggca gcccatgctt
     1801 tccttatccg aggtacacct caatgagcaa attcatcatt cagccgcact tccgtctgca
     1861 ggagtgggtc gcggaggaga agggctattt cgccgcggaa ggcctcgact acgtgttcaa
     1921 cgaggaggtc caggcgaccg acggcaaggt gcatgacaag ggcaagaagg tcggcgccta
     1981 tcagtctttc gaggccggcc gctcctccga tgtgagctgc gcctgtcact ggacggtcaa
     2041 cgtcgccgcc tcgaacggcc acggcaagat gatgaccgac gtc
//
LOCUS       DASRWS010000216         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2665885, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000216 DASRWS010000000
VERSION     DASRWS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665885"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..712
                     /locus_tag="VFF72_04030"
     CDS             275..712
                     /locus_tag="VFF72_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92359.1"
                     /translation="MTCRHFGGVGTIEVWRNSAKAVARGEAAWVGTFRADAEPGAISY
                     SAETQRALGIVAAPANWPTLCHIAGSLAGVLAAGTTDWATIESRVAADAGAVDPDSAP
                     WSAADIAQVDAILRGRWHQVQDEVETVVDDFKRSRRGRVMFKS"
     gene            complement(719..>2093)
                     /locus_tag="VFF72_04035"
     CDS             complement(719..>2093)
                     /locus_tag="VFF72_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV92360.1"
                     /translation="GETVSFVMPNGLQTLRLLLGALHAGRCVNPVNLLAQPEQMRYVL
                     DHSDCRLVVVAPEWVERVRAALAGIDRDIPLLVVAPDGDAADLIAALPAGTTGSGTVV
                     SRIAPAPAAGDTALLMYTSGTTGVPKGVMLTQGNLAANASAICREHGLGADDRVLGVL
                     PLYHINAFLVTMLAPLASGGSVAVAPRFSAAQFWSQAIERRCTWLNVVPTIVSYLLEG
                     DVPPRASIAGLRFCRSASAALPPEHHRAFEAKFGIGIVETMGLTETAAPSFSNPIVAS
                     ERRAGSVGRVSGGEARVVDASQATLADGQTGEIVIRGPHVMRGYYKNEAATRASFTAD
                     GWLRTGDLGHRDADGFFFVTGRIKELIIKGGENIAPREIDEVLLAHPAVLDAAAVGMP
                     DRHYGQDILACVVLRDGVACDVDSLRAFCSERLGRFKTPREIRFVASLPRGPSGKVQR
                     LKLLDPA"
BASE COUNT          325 a          723 c          769 g          276 t
ORIGIN      
        1 gttcctgttc gaagccggtc gctcggccga cacctccggg cactaatcct cgtaccgccg
       61 ggaacctgcg ccgcgcccgg tcaggttccc gcaaggcgcg ctcggccaga ctcggccgat
      121 gcatgagtgg gcaccgtcag ccgcgcggcc ggctcgcctc gtcgaggatg gccgcgtccg
      181 accggcgccc ggatgttctc ggaaggcgcg gcacgagcac gaagtcgccg aacgcgagtt
      241 gcgcgcggtg gcatggcgcg aggccggccg tgccgtgacg tgccggcact tcggcggcgt
      301 cggcacgatc gaagtctggc gcaactccgc caaggcggtg gcccgcggcg aggccgcctg
      361 ggtcggcacg ttcagggccg acgccgagcc cggcgcgatc agctactcgg ccgagacgca
      421 gcgggcgctg ggcatcgtcg cggcgccggc gaactggccc actctttgtc acatcgccgg
      481 cagtctcgcc ggcgtgctgg cggccggcac caccgattgg gcgacgatcg aatcacgggt
      541 ggcggctgac gccggcgcgg tcgacccgga cagcgcgcca tggagcgcgg ccgacatcgc
      601 ccaggtcgat gcgatcctgc gcggacgctg gcatcaggtg caagacgagg tcgagacggt
      661 cgtcgacgac ttcaagcgat cgcggcgcgg ccgcgtcatg ttcaagagct gacgacgttc
      721 acgcgggatc gagcagcttc aaccgctgca ccttgcccga cggaccgcga ggcagcgaag
      781 cgacgaaacg gatctcgcgc ggcgtcttga accggccgag gcgctcgctg cagaacgcgc
      841 gcaggctgtc gacgtcgcag gcgacgccat cgcgcaggac cacgcaggcg aggatgtcct
      901 ggccgtagtg gcgatcgggc atgccgaccg ccgcggcgtc gagcaccgcc gggtgggcga
      961 gcagcacttc gtcgatctcg cgcggggcga tgttctcgcc gcccttgatg atcagctcct
     1021 tgatgcggcc ggtgacgaag aagaagccat cggcgtcgcg gtggccgaga tcgccggtgc
     1081 gcagccagcc gtcggcggtg aagctggccc tcgtcgccgc ctcgttcttg tagtagccgc
     1141 gcatcacgtg cgggccgcgg atgacgatct cgcccgtctg cccgtcggcc agcgtcgcct
     1201 gggacgcatc gacgacgcgg gcctcgccgc ccgagacgcg gccgaccgat cccgcccggc
     1261 gctcgctcgc cacgatcggg ttggaaaacg aaggcgccgc cgtttcggtc agtcccatcg
     1321 tctcgacgat gccgatgccg aacttcgcct cgaaggcgcg atgatgctcg ggcggcaagg
     1381 ccgccgacgc ggagcggcag aagcgcaaac cggcgatcga tgcacgcggc ggcacgtcgc
     1441 cttcgagcag gtaagagacg atcgtcggca cgacattgag ccatgtgcac cgtcgctcga
     1501 tcgcctggct ccagaactgc gccgccgaga aacgcggcgc gacggcgacg ctgccgccgc
     1561 tcgcgagcgg cgcgagcatc gtcaccagga aggcgttgat gtgatacagg ggcaacacac
     1621 cgaggacgcg gtcgtcggcg ccgagcccgt gctcgcgaca gatggcggag gcattggcgg
     1681 cgaggttgcc ctgggtcagc atcacgccct tcggcacgcc ggtggtgccc gacgtgtaca
     1741 tcaggagcgc ggtgtcgccg gcggcgggcg cgggcgcgat gcgggagacc accgtgccgg
     1801 acccggtcgt gcccgccggc agcgccgcga tcaagtccgc cgcatcgccg tcgggcgcga
     1861 cgacgagaag cgggatgtcg cgatcgattc ccgcgagcgc ggcgcgaacg cgctcgaccc
     1921 actccggcgc gacgacgacg agccggcaat ccgagtgatc cagcacgtag cgcatctgct
     1981 cgggctgggc gagcaggttg accggattga cgcagcggcc ggcgtgcagc gcgccgagca
     2041 gcagtcgcag cgtctgcagg ccgttgggca tcacgaagga gacggtctcg ccg
//
LOCUS       DASRWS010000217         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3536581, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000217 DASRWS010000000
VERSION     DASRWS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536581"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..122)
                     /locus_tag="VFF72_04040"
                     /pseudo
     CDS             complement(1..122)
                     /locus_tag="VFF72_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
     gene            176..328
                     /locus_tag="VFF72_04045"
     CDS             176..328
                     /locus_tag="VFF72_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92361.1"
                     /translation="MALVRHQLRRIAHGKDPALGTHPAYAFACDNVDQTRALRRYRSW
                     LQDVPA"
     gene            complement(342..476)
                     /locus_tag="VFF72_04050"
     CDS             complement(342..476)
                     /locus_tag="VFF72_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92362.1"
                     /translation="MRESVAVAAAPTSVEERARVEAGERVEDARIDGDVMAEIDSVEA
                     "
     gene            complement(482..1864)
                     /locus_tag="VFF72_04055"
     CDS             complement(482..1864)
                     /locus_tag="VFF72_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92363.1"
                     /translation="MLVVVSIAQLMVVLDATVVNIALPTAQADLGFDNSQRQWIVTAY
                     ALAFGSLLLLGGRVGDIVGRKRTFLIALVAFAGASALGGAAPSFAVLVIARALQGVAG
                     AMLAPAALGTLVTTFKDPRDRGKAFGVFGTVAVGGGAVGLILGGILTEYLSWRWCMYV
                     NVLFAAAAVVGALVYMSEAKPPVRPRIDVGGTVLAGLGLFGLVFGFSQAETDGWGSSV
                     TMASLAIGVLLLVAFVRVEQRVGHPLLPLRVVSDRSRGMAFGAVGIAGVAMFGLFLFL
                     TYYLQLVKHFSPVTSGLAFLPMIAAIMVSSNTSNIVTLPRFGPRVVITAGMLTGFLGL
                     GYLSRINVHSSYAGSVLPALLLMGLAMGMVMAPSMNTATAGVQPQDSGVAAALVSTMQ
                     QVGGSIGTAVTSTIAASVTASYVSSHGAVRGFNAVASTHGYATVFLVSALVFAAGGIA
                     AAALFPPRRG"
     gene            complement(1926..>2136)
                     /locus_tag="VFF72_04060"
     CDS             complement(1926..>2136)
                     /locus_tag="VFF72_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92364.1"
                     /translation="IRSEDEKDRRARIVALTPAGRNTFEAAQVVRLKTMDEIFGSLSA
                     RERSSLAALVATIRTNLASGEDSCG"
BASE COUNT          381 a          748 c          731 g          276 t
ORIGIN      
        1 agatggcttc ggtagccgac tgcggcaaca gccgagagga gcgcgtctcg atcggcgtag
       61 tgccggtacg gcgcggacgg cgcgacgccg gcccggcggg cgacggcgcg cagcgagagc
      121 ccgctcgcgc cgccccgtca cgtggtcgga caagaccctt gcatcagcgg ccgggatggc
      181 gctggtccgt caccaactgc gccgcatcgc gcacggcaag gaccctgctc tcgggaccca
      241 cccggcgtac gcgttcgcct gcgacaacgt cgaccagacg cgcgcgctgc gccggtaccg
      301 ctcctggctg caggacgtgc ccgcctgagc gggcgatgac ttcaggcttc gaccgagtcg
      361 atctcggcca tcacgtcacc gtcgatgcgc gcgtcttcga cgcgctcgcc ggcttcgacg
      421 cgcgcgcgtt cttcgacgct cgtcggcgcg gcagcaacgg cgaccgactc gcgcatcgcg
      481 gtcagccgcg ccttggaggg aacagcgccg ccgccgcgat accgccggcg gcgaagacca
      541 gtgccgacac gaggaagacg gtggcgtagc cgtgcgtgct ggcgacggcg ttgaagccac
      601 ggaccgctcc gtgtgacgag acgtaggacg cggtcaccga ggcggcaatc gtgctcgtga
      661 ctgccgtgcc gatcgagccg ccgacctgct gcatggtgct gacgagcgct gccgcgacac
      721 ccgagtcctg cggctgcacc ccggctgtcg cggtgttcat cgacggcgcc atcaccatgc
      781 ccatcgcgag acccatcagg agcagcgcgg gcaggacgct gccggcgtag gacgagtgga
      841 cgttgatgcg gctgagatag cccagcccca ggaagccggt cagcatgccc gccgtgatca
      901 cgacgcgcgg cccgaaccgg ggcagcgtga cgatgttcga cgtgttcgag ctgaccatga
      961 tcgcggcgat catcggcagg aacgccaggc ctgaggtgac cggactgaag tgcttgacca
     1021 gctgcaggta gtaggtcagg aagaggaaca ggccgaacat cgccacgccg gcgatgccca
     1081 ccgcgccgaa ggccatcccg cgcgaacggt cagagaccac gcgcagcgga agcagcggat
     1141 ggcccacccg ctgctcgacc cggacgaacg cgaccagcag cagcacgcca atggcgagcg
     1201 acgccatcgt gacgctcgag ccccaaccgt ccgtctctgc ctgggagaag ccgaacacca
     1261 ggccgaacag gcccagaccc gcgagcaccg tgccaccgac gtcgatacgc ggacggaccg
     1321 gtggcttcgc ctcggacatg tagaccagcg cgccgacgac cgcagccgcg gcgaacagca
     1381 cgttgacgta catgcaccag cgccacgaca ggtactccgt gaggatgccg ccgaggatca
     1441 gaccgaccgc gccgccgccc accgcgaccg tgccgaagac cccgaaggcc ttgccgcggt
     1501 cgcgtggatc cttgaacgtc gtgacgagcg tgcctagcgc ggccggcgcg agcatcgcgc
     1561 ccgcgacgcc ctgcagcgct cgggcgatga ccagcaccgc gaacgagggc gcagccccac
     1621 cgagagccga cgcgccggcg aaggcgacga gcgcgatcag gaacgtccgc ttgcggccga
     1681 cgatgtcgcc gacccggccg ccgagcagca acaagctgcc gaatgcgagt gcgtaggcgg
     1741 tgacgatcca ctgccgctgg ctgttgtcga agccgaggtc ggcctgggcc gtcggaagcg
     1801 cgatgttcac gaccgtggcg tcgaggacga ccatcaactg ggcgatcgac acgacgacga
     1861 gcacgagcca ccgatggtcg gacccgccgc gcgcctctgc cgctttccgt tcggctattg
     1921 ctgtattagc cacagctgtc ttctcctgac gcaagattcg tacgaatggt ggcgaccagg
     1981 gccgccagtg aggagcgttc acgcgcagac aggctgccga agatctcatc catcgtctta
     2041 aggcgaacaa cctgcgcagc ctcgaaggta ttccggccgg ccggggtcaa tgcgacgatg
     2101 cgtgcgcgcc ggtctttctc gtcctcggag cggatg
//
LOCUS       DASRWS010000218         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_380979, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000218 DASRWS010000000
VERSION     DASRWS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380979"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFF72_04065"
     CDS             complement(<1..717)
                     /locus_tag="VFF72_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92365.1"
                     /translation="MVDLLVAAAPDGVRYEGKASEDYVLAVAPLSPHGGVLHEAPTTS
                     TGPQRPLFKALVQSGRSDFHQPGWEAPPTLAAARAARGRARPVGPLAQVPGQESVAAE
                     TARAEAAPGDRGLAATDYLLGKYLENGATTESFESTRIELERSSHRVRERVDVYEMYW
                     ADLSRLSGAIPRIVTELFTLVFRLSKLGRETVDEARLQLGARAPRWRWLAGTQMALDW
                     AFVNGLAALFAQLGLLGVAIV"
     gene            complement(1063..>2229)
                     /locus_tag="VFF72_04070"
     CDS             complement(1063..>2229)
                     /locus_tag="VFF72_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZV92366.1"
                     /translation="GPLFPLDTPPPTVVACLSCIVAGLAAGALSATLTGALYRIEDAF
                     AKLPVHWMWWPAIGGIVVGIGGYYEPRAFGVGYDVIGDLLQQHLALGVVAGLLVAKTV
                     MWSVALGSGTSGGVLAPLLMIGAGLGSLMGPWLPGDSPQLWALVCMAAVLGGTMRAPL
                     TAVVFAFGLTHDANAFLPTILACAVAYGFTVIVMPRSILTEKIARRGNHVFREYGVDP
                     LEKNFVGDVMTDEPIVIDGGLTVAAALAQHFGPAQRHRAYPVVVEGRWRGLVDRAFLQ
                     GLPAQALEKPLADVMTPAQDEAFALPTETCRSVAARMAALGVERLAVLDPDDRKLLVG
                     VVSRSDLLKPSRQLYEDEFRRERLLGRRARGVRGASRGKLGVDTGGGTPPGPAA"
BASE COUNT          358 a          804 c          767 g          300 t
ORIGIN      
        1 gacgatggcc acccccagca gaccgagttg cgcgaacagt gccgccaggc cgttgacgaa
       61 ggcccagtcg agcgccatct gcgtgccggc gagccaacgc cacctcggcg cccgcgcgcc
      121 gagctggaga cgcgcttcat cgaccgtctc ccggccgagc ttggagagcc ggaacaccag
      181 cgtgaacaac tcggtgacga tgcgcggaat cgcacccgac aggcgcgaca ggtcggccca
      241 gtacatctcg tagacgtcga cgcgttcgcg gacgcggtgg ctgcttcgtt cgagctcgat
      301 gcgcgtgctc tcgaaggact ccgtcgtcgc gccgttctcc aaatacttgc cgagcaggta
      361 gtcggtggcg gccaagccgc gatcccccgg ggccgcctcg gctctcgccg tttctgccgc
      421 aaccgattcc tgtcccggga cctgcgcaag cggcccaacg gggcgtgccc gtccgcgggc
      481 agcacgtgcc gctgccagtg tcggaggagc ctcccagcca ggctggtgga aatcggagcg
      541 tcccgattga accagggcct tgaacagcgg gcgctgcggt ccggtcgaag tcgtcggggc
      601 ttcatgaaga acacccccgt gaggcgacaa cggcgcgact gcgagcacgt agtcctcgct
      661 ggccttgcct tcgtagcgca cgccgtccgg cgccgcggca accaggagat cgaccaccga
      721 tcgcgcagtc tcgcccggcc gctggtcggc gactccgtgg accacgacga cggccacgcc
      781 gccccggcgc gcttgttcgt gcatcgccgc cgactcccca ggttgtgacg cggcgacctc
      841 ttcgagaccg ctgccgacag cgaccgtcgc ccccgcaggg gcaacagcga ccagccgtca
      901 tcatcccacg ccgcgccgat ccccgacgcc gcgagtccga cgctgcgccg accgaatcga
      961 tctcggttcc ggcgatgtga gcgtcgatgg cttaccgatg acgcgttcgt cgcgccaccg
     1021 cagcttcgtc acgcagcctt cgccccgacc cgcctcgctt tgctatgcag ccgggcccgg
     1081 cggcgtgccg ccgccggtgt cgacgccgag cttcccgcgg cttgcgccgc gcacgcctcg
     1141 cgcacgacga ccgagcagcc gctcgcgacg gaactcgtct tcgtagagct gtcgcgaggg
     1201 tttgagcagg tcgctgcggc tgacaacgcc gacgagcagc ttgcggtcgt ccgggtcgag
     1261 gacggcgagg cgctcgacgc cgagcgcggc catccgtgcc gccaccgagc ggcaggtctc
     1321 ggtgggcagc gcgaaggcct cgtcctgcgc tggcgtcatc acgtcggcca gaggcttctc
     1381 cagtgcctgc gcaggcaatc cctgcaggaa ggcgcgatcg acgaggccgc gccagcgtcc
     1441 ttccacgacg accgggtagg cgcgatgccg ctgcgccggg ccgaagtgct gcgccagcgc
     1501 cgcggcgacc gtgaggccgc cgtcgatgac gatcggctcg tccgtcatca cgtcgccgac
     1561 gaagttcttc tcgagcggat cgaccccgta ctcgcgaaag acgtggttgc cacggcgcgc
     1621 gatcttctcg gtgaggatcg agcgtggcat gacgatgacg gtgaagccgt acgcgaccgc
     1681 acaggcgagg atcgtcggca ggaatgcatt ggcatcgtgg gtgagtccga acgcgaagac
     1741 gaccgcggtg agcggcgcgc gcatcgtccc gcccaggacc gccgccatgc agacgagcgc
     1801 ccagagttgc ggcgagtcgc ctggaagcca cggccccatg aggctgccca ggccggcacc
     1861 gatcatgagg agcggcgcga gcacgccacc cgaggtgccc gagccgaggg cgaccgacca
     1921 catcaccgtc ttggcgacca ggaggccggc gacgacgccg agcgcgagat gctgctgcag
     1981 cagatcgccg atgacgtcgt agccgacgcc gaacgcgcgc ggttcgtagt agccgccgat
     2041 gccgacgacg atgccgccga tcgccggcca ccacatccag tggaccggca acttcgcgaa
     2101 tgcgtcctcg atgcgataga gcgcgccggt cagcgtcgcc gagagggcgc ccgcggcgag
     2161 gccggcgacg atgcacgaca ggcaggccac gaccgtcggc ggcggcgtgt cgagcggaaa
     2221 cagcgggcc
//
LOCUS       DASRWS010000219         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2963102, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000219 DASRWS010000000
VERSION     DASRWS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963102"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..400)
                     /locus_tag="VFF72_04075"
     CDS             complement(83..400)
                     /locus_tag="VFF72_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92367.1"
                     /translation="MRRHPLLIALLAGSTLLAGCDMLGIDSPEKVAAARDADGKAIGA
                     ACRQSARALEDCYAMYKRADRASVYAGWRDMNDYMRENKIESVTPQLAAQAGQAKGDG
                     QSH"
     gene            complement(708..1562)
                     /gene="htpX"
                     /locus_tag="VFF72_04080"
     CDS             complement(708..1562)
                     /gene="htpX"
                     /locus_tag="VFF72_04080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZV92368.1"
                     /translation="MFNMLKTAVLMAAITALFMAIGAFVGGQQGMLLALLVAVALNFL
                     SYWFSDTLVLKMYNARRVDGTSAPQFHRLIAELAGKAGLPMPRVYLIEEDAPNAFATG
                     RNPEHAAVAATTGILRILSERELRGVMAHELTHVAHRDILISTISATMAGAIGMLANF
                     AMFFGGRDENGRAANPLAGLLVALLAPLAAGLIQMAISRAREFEADRGGAELSGDPQA
                     LASALQKIQSYSRGVPMESAERHPATAQMMIMNPLHGGGWASLFSTHPPVEERVHRLM
                     AMATGVAR"
     gene            complement(1847..>2114)
                     /locus_tag="VFF72_04085"
     CDS             complement(1847..>2114)
                     /locus_tag="VFF72_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZV92369.1"
                     /translation="FDPFPGARGTLDGEVVTCWRGDLRDDAGQPPGQVVAVENDALVV
                     ACGKGSLALTELQRAGGRRLPAAEFLRGRTLAPGASFEVAPGGP"
BASE COUNT          321 a          712 c          784 g          297 t
ORIGIN      
        1 gagagcgggc agcgggtcgt cgtcggcgag ttcgtggtcg ccgggcaggc gccgtgagaa
       61 gaaagggcgg ggtcctggga aatcagtgcg actggccgtc gcccttggcc tggccggcct
      121 gcgcggcgag ctgcggcgtc accgactcga tcttgttctc gcgcatgtag tcgttcatgt
      181 cgcgccagcc ggcgtacacg gacgcccggt cggcccgctt gtacatggca tagcaatcct
      241 cgagggcgcg tgccgactgg cgacaggccg cgccgatcgc cttgccgtcg gcgtcgcgcg
      301 ccgcggcgac cttctcgggc gaatcgatgc cgagcatgtc gcagccggcc aggagcgtcg
      361 agccggcgag gagggcgatg agaagggggt gcctgcgcat gacgaacgaa ggagggttcg
      421 caagcgatat cggcggcagg gcgcgcgact tgaggccgcg ccggcggatc ccggtgaggt
      481 cctcggggca ccgaaacctt tgttcggagg acgccgcgcg cgtatctccg atgacgcgaa
      541 aaggccgacg cggcatgcga cgcgcgcgcg atgcgcgtcg cgcggcgcct gcgcgatgcg
      601 gcgatgcggc gatgcggtga tgcggtgatg cgacacctgc gcgatgcgcc tcgccggcct
      661 tcgcgccgat gctggcgcgg cttcttttcg gcaaaggcgc cggccgctca gcgcgcgacg
      721 ccggtcgcca tcgccatcag cctgtggaca cgctcctcga ccggcggatg cgtggaaaag
      781 agcgacgccc agcccccgcc gtgcagcggg ttcatgatca tcatctgcgc cgtcgccggg
      841 tgacgctcgg ccgactccat cgggacgccg cgtgaatacg actggatctt ctgcagcgcc
      901 gaagcgagcg cctgcggatc gcccgacagc tcggcgccgc cgcggtcggc ctcgaactcg
      961 cgcgcccggc tgatcgccat ctggatcagg ccggcggcga gcggcgcaag cagcgcgacg
     1021 aggaggccgg cgagcgggtt ggccgcccgt ccgttctcgt cgcggccgcc gaagaacatc
     1081 gcgaagttgg cgagcatgcc gatcgcaccc gccatcgtcg cgctgatcgt cgagatgagg
     1141 atgtcgcggt gcgcgacgtg cgtcagctcg tgtgccatga cgccgcgcag ctcgcgctcg
     1201 gacaggatgc gcaggatgcc ggtggtcgcg gcgaccgcgg cgtgctcggg gttgcggccg
     1261 gtggcgaacg cgttcggcgc atcttcctcg atgaggtaga cgcgcggcat cggcaagccg
     1321 gccttgccgg cgagctccgc gatcaggcgg tggaactgcg gcgccgaggt cccgtcgacg
     1381 cggcgcgcgt tgtacatctt cagcaccagc gtgtcggaaa accagtagct taagaagttg
     1441 agcgcgacgg ccacgagcag cgccagcagc atgccttgct ggccaccgac gaaggcgccg
     1501 atggccatga agagcgccgt gatcgcggcc atcaggacgg cggtcttgag catgttgaac
     1561 atcgtgagga acgtggcaag cggcgccaag acggcgacaa ggcctcagat gggcgcgaag
     1621 cgccggagtt caaatcgcat ccggcgtcgg ccgcactgcg gtccctccgt cgacctgcgc
     1681 ggcgttgcgt cgatagactg ccagactcgc gacgacagtg aaatcgggac accggcggca
     1741 ccggcaacgg gcgatcggca ccatgggcgc gatatcgggc aggtcggcgc cgacctcgcg
     1801 ctctgccgcg ctcttcgaca gcctgctgcg cgccggcgcg cagatctcag gggccgcccg
     1861 gcgccacctc gaaactcgct cccggcgcca gcgtgcgacc gcgcaggaac tcggccgcgg
     1921 gaaggcggcg gccgccggct cgctgcagct cggtcagcgc caggctgcct ttgccgcagg
     1981 cgacgaccag tgcatcgttc tcgacggcga cgacctgccc cggcggctgg ccggcgtcgt
     2041 cgcgcagatc gccgcgccag caggtgacga cctcgccgtc gagcgtgccg cgtgcgccgg
     2101 gaaacggatc gaat
//
LOCUS       DASRWS010000220         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2963322, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000220 DASRWS010000000
VERSION     DASRWS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963322"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFF72_04090"
     CDS             <1..649
                     /locus_tag="VFF72_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZV92370.1"
                     /translation="FDPVRDLQPVTLVGKVPFILAVTPSLPVHTLAEFIAYAKAHPHQ
                     LNYSSFGRNTSNHLAGELFKSMTQIDAVHVPYKGSAPSVTDLLAGQVQYTFDTPPALV
                     GHIQAGKLRALAVAASERLPSLPSVPTFAEAGGPAFVAGTWFGLLAPAKTPPAIVDRI
                     NAAVVAVLASPELTKAMADRGIVASPTSPAEFGRFIHAEIAKWKALAGKIGIVAE"
     gene            659..1873
                     /locus_tag="VFF72_04095"
     CDS             659..1873
                     /locus_tag="VFF72_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV92371.1"
                     /translation="MRTLLKNANLVDCVEPLARADTSVLIEDGRIREIRPSAQLSQVG
                     DAEVVDLAGDWLMPGLWDVHIHPDYLSLAEVPLADQVTLFGNRLMTALTESGIVGLRC
                     AGSHHFMDVAWKRAFDAGLYTGPRVFAAGHFLTTTGGHFLTSGHALECDGPYGFVKAI
                     REQIKNGVDHIKLNLSGGIMGPPWDLHRHSFLLDEELKAAFDVCRLREFKVMAHATNP
                     QAVKNAIRMGAHSVEHGYILDDECIELFLEHGTWYVPTLSISHLSPSQASNDWEKRWV
                     KQRNLSHALCCRVDAASGVHTEWFRKALAAGVKMALGSDIRPLHDAALLELGLWIRDG
                     ATPWQALQAATRNAAAVCGVGDELGTIEVGKRADLIVVAGDPLADIQNVRRLRLVLKD
                     GRIVADKRTRAP"
     gene            1892..3142
                     /locus_tag="VFF72_04100"
     CDS             1892..3142
                     /locus_tag="VFF72_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZV92372.1"
                     /translation="MAEAASPRVAVVGAGIVGSCIALALGKRGADVTLIDRDEPGRGC
                     SFGNSGAISPGSVAPLAMPGIVSSLPAMLLDPEGPLHLRLGYLPRALPWLLRFVASAR
                     PAEVARNAARLAALHAGAIDRHRALTRELGVPELFLQRGHLHLYPDEQALAKDAAGWR
                     LRTEYGIRFDKVDRDGILALEPGVGSRYQVGIFVADHATIVDPFRYVQAMVAAFVGRG
                     GNVRRAEVRALTRGDDGWRVTDGQDADADTRYAHVVVATGAWSKGLLSPLGIDVAIES
                     QRGYHVQFAGARDTVSRTVVLADRKIFVTPMAGGLRVGGTVEIAGLARPPVARRSALL
                     ERIARETFAGLDAIEPSRWMGHRPCTPDSLPIVGAAPGQSGLWLAIGHGHLGVTDSIV
                     TSERIADGILGDRPGADPFDKERP"
     gene            3139..>3367
                     /locus_tag="VFF72_04105"
     CDS             3139..>3367
                     /locus_tag="VFF72_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92373.1"
                     /translation="MSQPSRPISGLRLLVAAATLIAGSLAHAQAWPTKPVHFVIPFPA
                     GGSTDVVGRLIADRLAQSLKQPFVIENKGGAG"
BASE COUNT          495 a         1251 c         1108 g          513 t
ORIGIN      
        1 cttcgatccg gtccgcgacc tgcagcccgt gacgctggtc ggcaaggtgc ccttcatcct
       61 cgccgtcacg ccatccttgc cggtgcacac gctcgccgaa ttcatcgcct acgcgaaggc
      121 gcatccgcat cagctgaact actcgtcgtt cggcaggaac acctccaacc acctcgccgg
      181 cgagctcttc aagtcgatga cgcagatcga cgccgtgcac gtgccctaca agggcagcgc
      241 gccttcggtg accgaccttc tcgccgggca ggtccagtac accttcgaca cgccgccggc
      301 gctggtcgga cacatccagg ccggcaagct gcgcgcgctc gccgtcgctg ccagcgagcg
      361 gttgccgagc ctgccctcgg tgccgacctt cgccgaggcc ggcggcccgg ccttcgtcgc
      421 cggcacctgg ttcggcctgc tggcgccggc gaagacacca ccggcgatcg tcgaccggat
      481 caacgccgcg gtcgtcgccg tgctggcctc gcccgagctg acgaaggcga tggccgatcg
      541 aggcatcgtc gcttcgccga cgagcccggc agaattcggc cgcttcatcc acgccgagat
      601 cgccaagtgg aaggccctcg ccggcaagat cggcatcgtc gcggaatagc gcgctcccat
      661 gcgaacactg ctgaagaacg ccaacctcgt cgactgcgtc gagccgctgg cccgcgccga
      721 cacgagcgtg ctgatcgagg atggccgcat tcgcgagatc cgtccctcgg cgcaactctc
      781 gcaagtcggc gatgccgagg tcgtcgacct cgccggcgac tggctcatgc ccggcctctg
      841 ggacgtccac atccaccccg actacctgtc gctcgccgag gtgccgctcg ccgaccaggt
      901 gaccctcttc ggcaaccgcc tgatgacggc gctcaccgag tcgggcatcg tcggcctgcg
      961 ctgcgccggc agccatcact tcatggatgt cgcctggaag cgggccttcg atgccggcct
     1021 ctacaccggc ccgcgcgtct tcgcggccgg ccacttcctg acgaccacgg gcggccactt
     1081 cctcacctcc gggcacgcgc tcgagtgcga cggaccctac ggcttcgtca aggcgatccg
     1141 cgagcagatc aagaacggcg tcgaccacat caagctcaat ctctccggcg gcatcatggg
     1201 gccgccgtgg gacctgcacc gccattcctt cttgctcgac gaggagctga aggccgcctt
     1261 cgacgtctgc cggctgcgcg aattcaaggt gatggcgcac gcgacgaatc cgcaagcggt
     1321 gaagaacgcg atccggatgg gtgcgcacag cgtcgagcac ggctacatcc tcgacgacga
     1381 atgcatcgag ctcttcctcg agcacggcac gtggtacgtg ccgacgcttt cgatcagcca
     1441 cctttcgccg agccaggcga gcaacgattg ggaaaagcgc tgggtcaagc agcgcaatct
     1501 ctcgcatgcc ttgtgctgcc gcgtcgatgc cgcctcgggc gtgcacacgg agtggtttcg
     1561 caaggccctc gccgccggcg tgaagatggc gctcggctcc gacatccggc cgctgcacga
     1621 cgccgcgctg ctcgagctcg gcctgtggat ccgcgacggg gcgaccccgt ggcaggccct
     1681 gcaggcggcc acaagaaacg ccgccgcggt atgcggcgtc ggcgacgagc tgggcacgat
     1741 cgaagtcggc aagcgcgccg acctgatcgt cgtcgccggc gacccgctgg ccgacatcca
     1801 gaacgtgcgg cgacttcgcc tcgtcctcaa ggacggccgg atcgtcgccg acaagcgcac
     1861 gcgcgcgccg tgagcggccg ggaccggacg catggctgag gccgcttccc cgcgcgtcgc
     1921 ggtcgtcggc gccggcatcg tcggcagctg catcgcgctg gcgctgggaa agcgcggcgc
     1981 cgacgtgacg ctgatcgatc gcgacgaacc cggccgcggc tgcagcttcg gcaactcggg
     2041 cgcgatcagc cccggttcgg tcgcgccgct ggcgatgccc ggaatcgtct cgtcgctgcc
     2101 ggccatgctg ctcgaccccg agggcccgct gcacctgcgc ctgggctacc tgccgcgggc
     2161 cctgccgtgg ctgctgcgct tcgtcgcctc ggcgcggccc gcggaagtgg cccgcaacgc
     2221 ggcgcggctg gctgcgctcc atgccggcgc catcgacagg caccgggcgc tgacgcgcga
     2281 gctcggcgtg cccgagctct tcctgcagcg cggccacctc catctctatc ccgacgaaca
     2341 ggctctcgcc aaggacgcgg cgggatggcg tctgcgaacc gagtacggga tacgcttcga
     2401 caaggtcgac cgcgacggga ttcttgcgct cgagcccggt gtcggcagtc gatatcaggt
     2461 cggcatcttc gttgccgatc acgcgaccat cgtcgatccg ttccgctacg tgcaggccat
     2521 ggtcgccgcg ttcgtcggcc gtggcggcaa cgtcaggcgc gccgaagtgc gggcgctgac
     2581 gaggggcgat gacgggtggc gcgtgaccga cggtcaagac gccgatgccg acacgcgcta
     2641 cgcgcacgtc gtcgtcgcca cgggcgcgtg gtcgaagggc cttctctcgc cactcggcat
     2701 cgacgtcgcg atcgagagcc agcgcggcta tcacgtccag ttcgcgggcg ctcgcgacac
     2761 ggtgtcgcgc accgtcgtgc tggccgaccg caagatcttc gtcacgccga tggccggagg
     2821 gttgcgcgtc ggcggcacgg tcgagatcgc ggggctggcg cggccgcccg tggcccgccg
     2881 ctcggcgctg ctcgagcgga tcgcccgcga gaccttcgcc gggctcgacg cgatcgaacc
     2941 cagtcgctgg atgggccacc gcccgtgcac ccccgattcg ctgcccatcg tcggcgccgc
     3001 gccgggccag agcggcctgt ggctcgcgat cggccacggc cacctgggcg tcaccgattc
     3061 gatcgtcacc tccgaacgca tcgccgacgg catcctcggc gaccggcccg gcgccgatcc
     3121 attcgacaag gaacgaccat gagccagcca tcccgcccga tctccggcct tcgccttctc
     3181 gtcgccgccg cgacattgat cgccggctcg ctcgcccatg cgcaagcgtg gccgacgaag
     3241 cccgtgcatt tcgtcattcc cttcccggcg ggcggctcga ccgacgtcgt cggccggctg
     3301 atcgccgacc gtctggcgca gagcctgaag cagcccttcg tcatcgagaa caagggcggt
     3361 gccggcg
//
LOCUS       DASRWS010000221         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1804482, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000221 DASRWS010000000
VERSION     DASRWS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804482"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFF72_04110"
     CDS             complement(<1..1284)
                     /locus_tag="VFF72_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZV92374.1"
                     /translation="MAAGEGVRTACPYCGVGCGVIAVPAKAGGIDISGDPMHPSNFGA
                     LCSKGAALGETLGLDGRLLHPMVLRDGELQRADWTAALDAVATGFRRAIKQHGPDAVA
                     FYLSGQLLTEDYYVANKLMKGFIGSANVDTNSRLCMASSVAGHRRAFGADTVPGVYAD
                     LDEADLIVLVGSNAAWCHPVLFRRMAANQRERGARLVVIDPRRTATAEDVDLFLPIAP
                     GSDTALFCGLLTHLAETGTLDDAFIGDHTTGFAEAIARARAIAPDAEATARATGLALA
                     DVVSFFGWFRATPRAVTCYSQGVNQSAQGTDKVNAIINCHLATGRIGKPGMGPFSLTG
                     QPNAMGGREVGGLANQLAAHMGFSPAEIDRVRRFWKAPRMATHEGLKAVQMFEAIERG
                     EIKALWVMATNPALSLPRATAMQSALRKLDLFVVSD"
     gene            complement(1284..>2675)
                     /locus_tag="VFF72_04115"
     CDS             complement(1284..>2675)
                     /locus_tag="VFF72_04115"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HZV92375.1"
                     /translation="DGRPLRRRMMAAMGQQDCGQCGYSCEDYSNALFLKKEERVNLCV
                     PGGKDTLRMLKKLSEEFAAAAPVAAPAAVTAEVRATDAPPASALAPRPAPGRSRDNPV
                     EAILLKRTRLNKPGSNKETWHIDIDLSGAGLDYVVGDAFGIMPTNDPALVDTVIHTLG
                     APADFPIGGRTLRDVLIDGVSLAPAPDMLFQLFSYLTGGDRRLKAKALAGGEDPDRDA
                     ASLDVLAAIEKFPGIRPDPEAFIESLEPLQPRLYSIASSPKVDPGRVSLTVDAVRYDV
                     ARRRRLGVASTFLAERLREGQKLKVYVQKAHAFGLPADPSVPVIMVGPGTGVAPFRAF
                     LHERMATRAPGRNWLFFGHQRSDYDFFYADELAGLKAKGVLTRLSLAWSRDTDEKFYV
                     QDRMRQVGRDLWAWLADGAHLYVCGDAQRMAKDVERALVDIVAQHGVRSADEAVAFVA
                     DLKRNGRYQQDVY"
BASE COUNT          428 a          915 c          934 g          398 t
ORIGIN      
        1 gtccgacacc acgaacaggt cgagcttgcg gagtgccgat tgcatcgccg tcgcgcgcgg
       61 cagcgacaat gccggattgg tcgccatcac ccacagcgcc ttgatctcgc cgcgctcgat
      121 cgcctcgaac atctgcacgg ccttgagacc ctcgtgcgtc gccatgcgcg gcgccttcca
      181 gaagcggcgc acgcggtcga tctcggcagg cgagaagccc atgtgcgcgg cgagctggtt
      241 ggccaggccg ccgacctcgc gtccgcccat cgcattgggc tggccggtga gcgagaacgg
      301 gcccatgccg ggcttgccga tgcggcccgt cgcgagatga cagttgatga tggcgttgac
      361 cttgtcggta ccctgcgccg actggttgac gccctgcgag tagcacgtga cggcgcgcgg
      421 cgtggcgcga aaccagccga agaacgacac cacgtcggcg agcgcgaggc cggtcgccct
      481 ggcggtggcc tctgcgtcgg gcgcgatcgc gcgcgcccgc gcgatcgcct cggcgaagcc
      541 cgtggtgtgg tccccgatga aggcgtcgtc gagggtgccg gtctcggcga gatgggtgag
      601 cagcccgcaa aagagcgccg tgtcgctgcc aggcgcgatg ggaagaaaca ggtccacgtc
      661 ttcggcggtc gcggtgcggc gcgggtcgat caccacaagg cgagcaccgc gctcgcgctg
      721 gttggcagcc atgcggcgaa acagcaccgg atggcaccag gccgcgttcg agccgacgag
      781 cacgatgaga tcggcctcgt cgagatccgc atagacgccg ggcaccgtgt cggcgccgaa
      841 ggcgcggcga tggcccgcga ccgacgacgc catgcatagg cgcgaattgg tgtcgacgtt
      901 ggcggatccg atgaaacctt tcatcagctt gttggcgacg tagtagtcct cggtgaggag
      961 ctgcccggag agatagaacg cgaccgcatc gggcccatgc tgcttgatgg cccgcctgaa
     1021 gccggtcgcg accgcgtcga gcgccgccgt ccagtccgcc cgctgcagct cgccgtcgcg
     1081 cagcaccatc ggatgcagca gccggccgtc cagccccaac gtctcgccca gcgccgcgcc
     1141 cttcgagcac agcgcgccga aattcgacgg atgcatcgga tcgccggaga tatcgatgcc
     1201 gccggctttg gccggcaccg caatgacgcc gcagccgacg ccgcaatacg gacacgccgt
     1261 gcgcacgccc tcccctgcgg ccatcaatac acgtcctgct gatagcggcc gttgcgcttg
     1321 agatcggcga cgaacgcgac cgcctcgtcg gcggaccgca ccccgtgctg ggcgacgata
     1381 tcgacgagcg cccgctcgac gtccttcgcc atgcgctggg cgtcgccgca gacgtagaga
     1441 tgggcgccgt cggcgagcca cgcccacaga tcgcggccga cctgccgcat gcggtcctgc
     1501 acgtagaatt tctcgtcggt gtcgcgcgac cacgcgagcg agaggcgggt gagcacgccc
     1561 ttggccttca ggccagcgag ctcgtcggcg tagaagaaat cgtagtccga gcgctggtgg
     1621 ccgaagaaca gccagttgcg gccgggcgcc ctggtcgcca tgcgctcgtg caggaacgcg
     1681 cggaacggcg cgacgccggt gccgggcccg accatgatga ccggcaccga tggatcggcc
     1741 gggaggccga aggcgtgcgc cttctgcaca tagaccttca gcttctgccc ttcgcgcagg
     1801 cgctcggcga gaaacgtcga tgcgacgccg agccggcgcc ggcgcgcgac gtcgtagcgt
     1861 accgcatcga ccgtgagcga gacgcgcccg ggatcgacct tcggcgacga cgcaatcgag
     1921 taaagccgcg gctgcagtgg ctcgagcgac tcgatgaagg cctcgggatc gggccgtatg
     1981 ccggggaatt tctcgatcgc ggcgagcacg tcgaggctcg cggcatcgcg atccggatcc
     2041 tcgccgccgg cgagcgcctt cgccttcagc cgccgatcgc cgcccgtcag ataggagaag
     2101 agctggaaca gcatgtcggg cgccggggcg agcgagacgc cgtcgatcag cacgtcgcgc
     2161 agcgtgcggc cgccgatggg aaaatcggcc ggtgccccca gcgtgtggat caccgtgtcg
     2221 accagcgccg ggtcgttggt gggcatgatg ccgaaggcat cgcccacgac gtagtcgagc
     2281 ccggcgccgg agaggtcgat gtcgatgtgc caggtttcct tgttcgagcc gggcttgttg
     2341 agccgcgtgc gcttgagcaa gatggcttcg accggattgt cgcgcgacct cccgggcgcg
     2401 ggtcgtggcg ccagtgccga tgcaggcggc gcatcggtcg cgcgcacttc cgccgtcacc
     2461 gccgcgggag cggcgaccgg ggcagcggcc gcgaactcct cgctcagctt cttgagcatg
     2521 cgcagcgtgt ccttgccgcc gggcacgcac aggttcactc gctcttcttt tttgagaaaa
     2581 agcgcgttcg aataatcttc gcacgagtaa ccgcactgcc cgcagtcctg ctggcccatc
     2641 gcggccatca tccgccggcg cagcggccgg ccgtc
//
LOCUS       DASRWS010000222         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1237291, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000222 DASRWS010000000
VERSION     DASRWS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237291"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /gene="sdhA"
                     /locus_tag="VFF72_04120"
     CDS             complement(<1..1348)
                     /gene="sdhA"
                     /locus_tag="VFF72_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZV92376.1"
                     /translation="MAGPAVHTSSVPTRRFDVVIVGAGGSGMRASLQLARAGLNVAVL
                     SKVFPTRSHTVAAQGGIGASLGNMSEDTWHYHFYDTVKGGDWLGDQDAIEFMCRAAPH
                     AVYELEHFGMPFDRNPDGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQQ
                     NVKARTNFFVEWMALDLIRDSEGAVVGVTALEMETGQLHVLHAKAVLLATGGAGRIFA
                     ASTNAYINTGDGLGMAARAGIPLEDMEFWQFHPTGVYGAGVLLTEGCRGEGAILRNAD
                     GERFMERYAPTLKDLAPRDFVSRCMDQEIKEGRGCGPEKDYIVLDMTHLGADTIHKRL
                     PSVLEIGHNFANVDITREPIPVVPTIHYQMGGVPTNLHGQVVAPRDGDPNAVVEGLYA
                     VGECSCVSVHGANRLGTNSLLDLLVFGRAAGNHIVEFNDKLKEHKELPADAADRTLE"
     gene            complement(1348..1710)
                     /gene="sdhD"
                     /locus_tag="VFF72_04125"
     CDS             complement(1348..1710)
                     /gene="sdhD"
                     /locus_tag="VFF72_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZV92377.1"
                     /translation="MAPTYGSRRLVVGAHYGLRDWLSQRITAIVMAVFSIVVVVQLLM
                     PGPIDYDRWSAMFAHQWMKVLTFIAVVSLLWHAWVGMRDIWMDYAKPVSVRLGVEVLT
                     LAWLVGCGGWAVQVLWRL"
     gene            complement(1721..2164)
                     /gene="sdhC"
                     /locus_tag="VFF72_04130"
     CDS             complement(1721..2164)
                     /gene="sdhC"
                     /locus_tag="VFF72_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZV92378.1"
                     /translation="MATSLKEQPLRTAPVYRNLRITQIASYRLPAAGWASIVHRISGA
                     LLFILLPLTLWMFDASVTSEVSFEQFANVFAFGIGPVPAWLFKIVVFGLIWAYLLHLC
                     IGLRYLWMDATHAVGKEFGRSSALVAMAVGTLVALALGAKLFSLY"
     gene            2299..>2552
                     /locus_tag="VFF72_04135"
     CDS             2299..>2552
                     /locus_tag="VFF72_04135"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746577.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZV92379.1"
                     /translation="MSKPPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLAGMTAHSDPLTAFKDA"
BASE COUNT          458 a          822 c          859 g          413 t
ORIGIN      
        1 gctccaatgt gcggtcggcg gcgtcggccg gcagttcctt gtgctccttg agcttgtcgt
       61 tgaactcgac gatgtggttg ccggccgcgc ggccgaacac cagcagatcg agcagtgagt
      121 tggtgcccag gcggttcgcg ccgtgcacgc tcacgcacga gcattcgccg acggcataga
      181 ggccctcgac gacggcgttc gggtcgccgt cgcgcggcgc gacgacctgg ccgtgcaggt
      241 tggtcggcac gccgcccatc tggtaatgga tcgtcggcac gaccgggatc ggctccctcg
      301 tgatgtcgac gttggcgaag ttgtggccga tctccagcac gctcggcagc cgcttgtgga
      361 tcgtgtcggc gcccaggtgc gtcatgtcga ggacgatgta gtccttctcg ggaccgcagc
      421 cacgtccttc cttgatttcc tggtccatgc agcgcgagac gaaatcgcgc ggcgccagat
      481 ccttcagcgt cggcgcataa cgctccatga agcgctcacc atcggcgttg cgcaggatcg
      541 ctccctcgcc gcggcagccc tcggtgagca ggacgccggc accgtagaca ccggtcgggt
      601 ggaactgcca gaactccatg tcctcgagcg gaatgccggc gcgtgccgcc atgcccaggc
      661 cgtcgccggt gttgatgtag gcgttggtcg aggcggcgaa gatgcggccc gccccgccgg
      721 tggcgagaag aacggccttg gcgtgcagga cgtgcaactg cccggtttcc atctcgagcg
      781 ccgtgacgcc gacgaccgcg ccttcgctgt cgcggatcag gtcgagcgcc atccactcca
      841 cgaagaagtt ggtgcgggcc ttgacgtttt gctgatagag ggtatgcagc atcgcgtggc
      901 cggtgcggtc ggccgcggcg caggcacgct ggaccggctt ttcgccgtag ttcgcggtat
      961 ggccgccgaa cggccgctga tagatcgtgc cgtcgggatt gcggtcgaac ggcatcccga
     1021 aatgctcgag ctcgtagacg gcgtgaggcg ccgcccggca catgaactcg atcgcgtcct
     1081 ggtcgcccag ccagtcgccg cccttgacgg tgtcgtagaa gtggtagtgc caggtgtcct
     1141 cgctcatgtt gccgagcgac gcgccgatgc cgccctgggc ggcaacggtg tgggatcggg
     1201 tcgggaacac cttggagagc acggcgacgt tgaggccggc gcgcgccagt tgcagcgagg
     1261 cgcgcatgcc ggagccgccc gcgccgacga tgacgacgtc gaaacgccgg gtcggaaccg
     1321 atgacgtgtg gacggcgggc cccgccatca cagcctccac aggacctgca ccgcccagcc
     1381 gccgcagccg acgagccacg ccagcgtcag cacctcgacg ccgaggcgaa cgctgaccgg
     1441 cttggcgtaa tccatccaga tgtcgcgcat gccgacccac gcgtgccaga gcagcgagac
     1501 gacggcgatg aaggtcagca ccttcatcca ttgatgcgcg aacatcgccg accagcggtc
     1561 gtagtcgatc ggtcccggca tgagcagctg gacgacgacg acgatcgaga acaccgccat
     1621 cacgatcgcc gtgatgcgtt gcgagagcca gtcgcgcaga ccatagtgcg cgccgacgac
     1681 gagacgcctg gaaccgtagg tgggagccat ctcgcgggac tcagtagaga gaaaagagct
     1741 tggcgccgag agcgagggcg accaaggtgc cgacagccat cgccaccagg gccgaacttc
     1801 ggccgaattc cttgccgacg gcatgcgtgg cgtccatcca caggtacctg aggccgatgc
     1861 acaggtgcag caggtaggcc cagatcaggc cgaagacgac gatcttgaag agccacgccg
     1921 gaacggggcc gatgccgaat gcgaaaacgt tggcgaactg ctcgaaggaa acttccgacg
     1981 tgacgctcgc gtcgaacatc cagagggtga gcggcaacag gatgaacagg agggcgccgc
     2041 tgatgcgatg gacaatcgaa gcccagccgg cggccggcag gcgatacgat gcgatctgcg
     2101 tgatgcggag gttgcggtag acgggagctg tcctgagcgg ttgttccttg agcgacgtgg
     2161 ccatgtcaac cttttccgtt ccgcgcgaag ccccgcgcag ccattcgagt ttattgcaac
     2221 gcacaagact ttaaccgcgg ccgtcgcgtc gcgcgcgaac ggtccttcat taccaacctc
     2281 acgtatcccg gagttttcat gagcaagccc cccgtccgag tcgcagtcac cggtgccgcc
     2341 ggccagatcg gatatgccct cctctttcgc attgcgtcgg gcgagatgct cggcaaggac
     2401 cagccggtca tcctgcagtt gctcgagatt cccgacgaga aggcgcagaa ggcgctcaag
     2461 ggcgtgatga tggagctcga cgactgcgcg ttcccgctcc tcgccggaat gacggcccac
     2521 agcgatcccc tgacggcctt caaggacgcc ga
//
LOCUS       DASRWS010000223         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_382535, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000223 DASRWS010000000
VERSION     DASRWS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382535"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..737
                     /locus_tag="VFF72_04140"
     CDS             9..737
                     /locus_tag="VFF72_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV92380.1"
                     /translation="MSDRKSILVIGAGDSTGGAIARRFAREGYAACVTRRTADKLEPL
                     VTQIQAAGGEAHGFASDARKEVDMQTLVAHVEREIAPIEVAVYNVGANVRFGIAETTE
                     RVYRKVWEMGALGGFLMGREVARAMLARGRGTIIFTGATASMRGSAGFAAFAGAKHAL
                     RALAQSMARELGPRGIHVAHVVIDGAIDTAFIAENFPERYAKKADAGILDPEAIAENY
                     WHLHGQAKSAWTHELDLRPWLESW"
     gene            complement(894..1733)
                     /gene="kynA"
                     /locus_tag="VFF72_04145"
     CDS             complement(894..1733)
                     /gene="kynA"
                     /locus_tag="VFF72_04145"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HZV92381.1"
                     /translation="MPTSDDAAGPGGAKVDFSRDMSYGDYLHLDEVLGAQHRRSGAHD
                     EMLFIIQHQVSELWMKLLLHELGAAIASIAADHLQPAFKMLARVTKIMEQMVHAWDVL
                     ATMTPPEYSQMRPSLASSSGFQSWQYRMIEFSLGNKNAAMLKAHSHRPDLLARVRAAH
                     EAPSLYDESLRLLARRGLPVPADHVERDWSQPYVASDGVEQAWLVVYRDPAAHWDLYQ
                     LGEELTDLEDIFRLWRFRHVTTVERIIGFKRGTGGTAGVSYLRKMLDVVLFPEIWKLR
                     TDL"
     gene            complement(1763..3061)
                     /gene="kynU"
                     /locus_tag="VFF72_04150"
     CDS             complement(1763..3061)
                     /gene="kynU"
                     /locus_tag="VFF72_04150"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZV92382.1"
                     /translation="MPGSFSREAALARDAADPLAPLRDAFSLPEDVVYLDGNSLGALP
                     RRSAERVRRTIEEEWGRGLIRSWNDAGWIDLSRSIGDRIAALIGAGAGEVSVGDSTSV
                     NLYKVLCAAATLQGLVDPRRTTIVSERSNFPTDLYIAESVAASTGWKVRLTDADKLVD
                     QLGRDTAVLMLTHVNYRTGRMHDMVETTRVAQASGALVVWDLAHSAGAVPVDLHGANA
                     DFAIGCGYKYLNGGPGAPAFVWAHSKHTARMDREQLRQPLSGWLGHAAPFEFATAYRP
                     AEGIARFVCGTPPIVAMRALESGLEVFAEAERFGGMAALRHKSVALTELFIERVEARC
                     AGHGLSLVTPREAVMRGSQASFAHDDGYPIMQALIERGVIGDFRAGDAANGEPNLLRF
                     GFTPLYTRHVDAWDAAEVLADILESGIWRQARFSQRAAVT"
BASE COUNT          537 a         1179 c         1227 g          536 t
ORIGIN      
        1 gcaagcgggt gagcgacagg aaatccattc tcgtcatcgg cgccggcgat tcgaccggcg
       61 gcgccatcgc ccggcgcttt gcacgcgaag gctatgccgc ctgcgtcacc cggcgcaccg
      121 ccgacaagct cgagccactc gtgacgcaga tccaggcggc aggcggcgag gcgcacggct
      181 tcgcatcgga cgcgcgcaag gaagtcgaca tgcagacgct cgtcgcccac gtcgagcgcg
      241 agatcgcgcc gatcgaggtc gcggtctaca acgtcggcgc caacgtccgc ttcggcattg
      301 ccgagaccac cgagcgcgtt taccgcaagg tctgggaaat gggtgcgctc ggcgggttcc
      361 tgatgggccg ggaagtcgcc cgggcgatgc tcgcgcgcgg tcgcggcacg atcatcttca
      421 ccggagcgac ggcgagcatg cggggctcgg ccggcttcgc cgcgttcgcc ggtgccaagc
      481 atgcgttgcg ggcgctggcg cagagcatgg cgcgcgagct cgggcccagg ggcatccacg
      541 tggcccacgt cgtcatcgac ggcgccatcg acaccgcctt catcgccgag aattttcccg
      601 agcgctacgc gaagaaggcc gacgccggca tcctcgaccc cgaagccatc gccgagaact
      661 actggcacct gcacggccag gcgaaaagcg cgtggacgca cgagctcgat ctgcgtccct
      721 ggctcgagtc ttggtgaacg cgtgaacact tccggcaccg cggccgccgc ggacggcacc
      781 ggcgccgcac gcgagccggc cgccgatgcg cggtcgctgg ggtatctctt cgacgtcggc
      841 agcccgacct cgtaactcgg ggcctcgcaa ccgccgcgcc ctgttccgtg ccgctacagg
      901 tcggtgcgca gcttccagat ctccggaaag agcacgacgt cgagcatctt gcgcaggtag
      961 ctcacgccgg cggtgccgcc ggtgccgcgc ttgaagccga tgatgcgctc gaccgtcgtt
     1021 acgtgacgga agcgccagag gcggaagatg tcctcgaggt cggtgagctc ctcgccgagc
     1081 tgatagaggt cccagtgggc ggccggatcg cgatagacga cgagccacgc ctgctcgacc
     1141 ccgtcgctgg ccacgtaagg ctgcgaccag tcgcgctcga cgtgatcggc cggcacgggc
     1201 aggccgcggc gggcgagcag gcgcagcgac tcgtcgtaga gcgaaggcgc ttcgtgcgcg
     1261 gcccgcacgc gggcgagcag gtcgggtcgg tggctgtgcg ccttgagcat cgccgcgttc
     1321 ttgttgccga ggctgaactc gatcatccgg tattgccagc tctggaagcc gctgctcgag
     1381 gcgagcgacg gccgcatttg cgaatactcg ggcggcgtca tcgtcgccag cacgtcccag
     1441 gcgtggacca tctgctccat gatcttggtc acccgcgcca gcatcttgaa cgcgggctgc
     1501 agatggtcgg cggcaatcga tgcgatcgcc gcgcccagct cgtgcagcaa aagcttcatc
     1561 cacagctcgc tcacctggtg ctggatgatg aagagcatct cgtcgtgagc cccggagcgg
     1621 cgatgctgtg cgccgagcac ttcgtcgaga tgaaggtagt cgccgtagct catgtcgcgg
     1681 ctgaaatcga ccttcgcgcc accggggccg gcggcatcgt cgctcgtcgg catcgcgccg
     1741 gtggcgtctc ggtcgccgct catcacgtca ccgcggccct ttgactgaag cgcgcttgcc
     1801 gccagatgcc gctctcgagg atgtcggcga gcacctcggc cgcgtcccat gcgtcgacgt
     1861 gacgggtgta gagcggcgtg aagccgaagc gcagcaggtt cggctcgccg ttcgccgcgt
     1921 cgccggcgcg gaaatcgccg atgacgccgc gctcgatcag cgcctgcatg atcggatagc
     1981 cgtcgtcgtg ggcgaagctc gcctggctgc cgcgcatcac cgcctcgcgc ggcgtcacga
     2041 ggctcaggcc atggccggcg caacgcgctt cgacgcgttc gatgaagagc tcggtcaggg
     2101 cgacggattt gtggcgaagt gccgccatgc cgccgaaacg ttcggcttcg gcaaagactt
     2161 cgaggcccga ttccagcgcc cgcatcgcga cgatgggagg tgtgccgcag acgaagcggg
     2221 cgatgccctc ggccgggcga taggcggtcg cgaactcgaa cggcgcggcg tggccgagcc
     2281 agcccgaaag cggctgtcgc agctgctcgc gatccatccg tgccgtgtgc ttcgaatgcg
     2341 cccagacgaa ggccggggcg ccgggccctc cgttgaggta cttgtagccg cagccgatgg
     2401 caaagtcggc gttcgcaccg tgcaggtcga cgggcacggc accggccgaa tgggcgagat
     2461 cccagacgac gagcgcgccg ctcgcctgcg cgacgcgcgt cgtctcgacc atgtcgtgca
     2521 tgcgaccggt gcggtagttg acatgggtca gcatcagcac cgccgtgtcg cggccgagct
     2581 ggtcgacgag cttgtcggcg tcggtcaggc gcaccttcca tccggtcgat gcggcgacgc
     2641 tttcggcgat gtagaggtcg gtcggaaagt tgcttcgctc ggacacgatc gtcgtgcgcc
     2701 gcgggtcgac caggccctga agggtcgccg cggcgcacag caccttgtag aggttgaccg
     2761 aggtcgaatc gccgacgctc acttcgccgg cgccggcgcc gatcaacgcg gcgatgcgat
     2821 cgccgatcga tcgtgaaaga tcgatccagc cggcgtcgtt ccagctgcgg atcaggccgc
     2881 gaccccattc ctcctcgatc gtccggcgca cccgttcggc gctgcgccgt ggcagcgccc
     2941 ccagcgaatt gccatcgagg tagacgacgt cttcgggcag gctgaaggcg tcgcgcaacg
     3001 gcgccagcgg atcggccgca tcgcgggcga gcgccgcctc gcgcgagaag cttccgggca
     3061 tcagcgactc cgcggccggg cccgcggcgg cgaggcgccg aaagccgaac ggcagctcgc
     3121 cggcgcgatc cgcagccgag gcgctatcgt cggcgcctgc tgccgcgcgc ggtggcgcaa
     3181 ttcatcggac agttctgacc ctggcgcggc ggcgcagatg tcgcatggcc ttttttcgtt
     3241 catgacgaca gtgacggtag caaaggatga agcggcgtgg tgtcggttaa atcggacacc
     3301 cgcgtgagcc gcgttgccgc ggcggcggcg atgcgccgcg aagcgcccgg cctggggccg
     3361 agaacgcttc gccggtgaag gcgatgagcc cggccgagcg ctggctgctc agcgcctatt
     3421 tcgtcgtcgt ctggggcgcc ggcttcgtcg ccacccgcgt ggcgatgatc tacgcggcg
//
LOCUS       DASRWS010000224         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2104156, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000224 DASRWS010000000
VERSION     DASRWS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104156"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFF72_04155"
     CDS             <1..865
                     /locus_tag="VFF72_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92383.1"
                     /translation="IWPHMSVFDNVAYPLQVRGMGRREIADKVAGVLRFVQLEGVERR
                     SATRLSGGQQQRVALARALAAEPKVILFDEPLSNLDAKLREETRKELKTFLGKLKITA
                     VYVTHDRVEALALSDDIAVMRAGRIVEIGTPQQIYFHAQHRFVADFIGRANLIGATVR
                     GHDDATTFVDTPLGPIACERRDLAVGSEATLCIRPEFMRVAAGARAGDDNVNGGKVGD
                     GSIGDGSVGGGNVIRGVVESLAFVGEVYEAEVRTGGLLLLARIDPGVDIHEGDAVSLC
                     VDPAHCLLVSR"
     gene            888..2576
                     /locus_tag="VFF72_04160"
     CDS             888..2576
                     /locus_tag="VFF72_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZV92384.1"
                     /translation="MRPSRPALTSTLIVIVALLTVAPVLMLAFGSFSQGLNAFGHFTL
                     AKYAAAYTDPALLRVIANTLVFVVGSTIVATGLALFLAYLNTRTDIPFKFLFGVLAIV
                     PMMIPHLLFSVSWGLLLNPSNGLVNRALQDAFSLQDAPFNIYSLPGMILVEGLLNMPV
                     AYLIVAPAMASFDVSLEESSRVFGAGTWRTLMRVTLPVLRPAILAAVVLGVVRSLASY
                     AVPRVLGTPGRVDVLATYLYGMIATGYSPDYGKAAALGMSVLATSIVLIWAYRRLTAE
                     SGRFVTISSRGWRPTVIELKGMRMPLFVVVALLSFVVVVLPVAVLLYTSFVPYSMVPG
                     AAAFAQMSLQHWREVIGDPTSRLALRNSVFLGVVGATLGVALSLFVAYVIVKVRTRAS
                     GLLETLTYLSFSFPGIVIGIGFMWFFVRTPLYATLTALLVGYIATYLPYGVRPLASAF
                     VQVHAHLEESSLVCGAGPLTTMRRIIVPLLVPGVVSAWILMATMFLRELTVSVVLSRP
                     GSEVLAVQILGFADDGLWGKLSALGIIMIVISTALVLLATFAGARFKAAQASAG"
BASE COUNT          339 a          941 c          889 g          484 t
ORIGIN      
        1 gatctggccg cacatgagcg tcttcgacaa cgtcgcctac ccgctgcagg tccgcggcat
       61 gggccggcgc gagatcgccg acaaggtggc gggcgtgctg cgtttcgtgc agctcgaagg
      121 cgtcgagagg cgctcggcga cgcgcctctc gggcggccag cagcagcgcg tcgccctcgc
      181 ccgggcgctc gccgccgagc cgaaggtgat cctcttcgac gaaccgctca gcaacctcga
      241 cgccaagctg cgcgaggaaa cgcgcaagga gctgaagacc ttcctcggca agctgaagat
      301 caccgccgtc tacgtgacgc acgaccgggt cgaggcgctg gcgctctcgg acgacatcgc
      361 ggtgatgcgc gccggccgca tcgtcgagat cggcacgccg cagcagatct acttccacgc
      421 ccagcatcgc ttcgtcgccg acttcatcgg ccgcgccaac ctgatcgggg cgacggtgcg
      481 cggccacgac gacgcgacga ccttcgtcga cacgccgctc ggcccgatcg cctgcgaacg
      541 gcgcgacctg gccgtgggca gcgaggcgac gctgtgcatc cggcccgagt tcatgcgcgt
      601 cgccgcgggt gcgcgcgccg gggacgacaa cgtcaacggt ggcaaggtcg gcgatggcag
      661 catcggcgat ggcagcgtcg gcggtggcaa cgtcatccgc ggcgtcgtcg agtcgctggc
      721 tttcgtcggc gaggtctacg aggcggaggt ccggacgggc ggcctgctgc tgctcgcgcg
      781 catcgacccg ggcgtcgaca tccacgaggg cgatgccgtg agcctctgcg tcgacccggc
      841 gcactgcctg ctcgtgtcgc gataggccgc tgcccgaacg catccgaatg agaccgtcgc
      901 gcccggcgct gacctcgacg ctgatcgtca tcgtcgcctt gctgacggtg gcgccggtgc
      961 tgatgctcgc gttcggcagc ttctcgcagg ggctgaacgc gttcggccac ttcacgctcg
     1021 ccaagtacgc cgccgcctac accgatccgg cgctgcttcg agtcatcgcc aacaccctcg
     1081 tcttcgttgt cggctcgacg atcgtcgcga ccgggctcgc gctctttctc gcctatctca
     1141 acacgcgcac cgacattccg ttcaagttcc tgttcggcgt gctggcgatc gtgccgatga
     1201 tgattccgca cctgctgttc tcggtgagct ggggactgct cctcaacccg tcgaacggcc
     1261 tcgtcaaccg ggcgctgcag gacgccttct cgctgcagga cgcgccgttc aacatctatt
     1321 cgctgcccgg catgatcctg gtcgagggcc ttctcaacat gcccgtcgcc tatctcatcg
     1381 tcgccccggc gatggcgtcg ttcgacgtgt cgctcgagga gtcgtcgcgc gtcttcggcg
     1441 ccggcacctg gcgcactctg atgcgagtca cgttgccggt gctcaggccg gcgatcctcg
     1501 cggcggtcgt gctcggcgtg gtgcgcagcc tggcgtcgta cgcggtgccg agggtgctcg
     1561 gcacgcccgg ccgcgtcgac gtgctggcga cctacctgta cggaatgatc gccaccggct
     1621 actcgccgga ctacggcaag gcggccgcgc tcggcatgag cgtgctggcg acgtcgatcg
     1681 tcctgatctg ggcctaccgc aggctcaccg ccgagagcgg ccggttcgtc accatctcga
     1741 gccgcggctg gcggccgacg gtgatcgagc tcaagggcat gcgcatgccg ctcttcgtcg
     1801 tcgtcgcgct gctgtcgttc gtcgtcgtcg tgctgccggt ggcggtgctg ctctacacct
     1861 cgttcgtgcc gtactcgatg gtgcccggcg ccgcggcctt cgcgcagatg agccttcagc
     1921 actggcgcga ggtgatcggc gacccgacat cgcggctcgc gttgcgcaac agcgtctttc
     1981 tcggcgtcgt cggcgcgacg ctcggcgtgg cgctctcgct cttcgtggcc tacgtgatcg
     2041 tcaaggtgcg aacccgcgcc tcgggcttgc tggaaacgct gacctacctg tcgttctcgt
     2101 ttcccggcat cgtcatcggc atcggcttca tgtggttctt cgtccggacg ccgctctacg
     2161 cgacgctgac cgcgctgctc gtcggctaca tcgccaccta cctgccgtac ggcgtgcggc
     2221 cgctggccag cgccttcgtg caggtgcacg cgcacctcga ggagtcgtcg ctggtgtgcg
     2281 gcgccgggcc gctgacgacg atgcgccgca tcatcgtgcc gctgctcgtt cccggcgtgg
     2341 tctcggcctg gatcctgatg gcgacgatgt tcctgcgcga gctcaccgtc tcggtcgtgc
     2401 tgtcgcggcc cggcagcgag gtgctggcgg tgcagatcct gggctttgcc gacgatggcc
     2461 tgtggggcaa gctctcggcc ctcggcatca tcatgatcgt catctcgacc gcgctcgtgc
     2521 tgctcgcgac ctttgccggg gcgcgcttca aggcggcgca ggcaagcgcc gggtagggcc
     2581 cggcgccgcg acgttcaata ccgcttggcc acctcgtcga gcatcacctc ggtcgcgccg
     2641 ccgccgatcg cga
//
LOCUS       DASRWS010000225         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_382966, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000225 DASRWS010000000
VERSION     DASRWS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382966"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1449
                     /locus_tag="VFF72_04165"
     CDS             181..1449
                     /locus_tag="VFF72_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV92385.1"
                     /translation="MSDPLAAPPSGAVPPVEGGTPSIAAPPSGAAERARAGRIARIVV
                     AVVVVLLIVGAGRTVIARIANSRVLEADAAAQSVQYVRTTFAKDSGAAQTLALPGTLQ
                     GWQQAPIAARATGYVKRWTHDIGSHVAKGELLAEIESPEVDQQLSQAVAARQQMTASQ
                     GLARSTAARWEALRKKDVVSQQELDEKRSAVAQADANLAAANANVQRLRQMQAFTRVT
                     APFAGVITRRSVDTGDLVDSAGRVLFVLTQMDPLRVYVNVPQSYAQLVRRGQKVVVTQ
                     PELRGRSFAGEVARTADSIDAASRTMQVEINLHNGDGALLPGAYVQVALPLAASATLV
                     VPTNVLLFRGDGTRVAVVDGAGHVRLRPVTLGRNLGQEIEVLDGIGPADRLVVNPSDS
                     LADGDTVQVAAADPAQAASGGAPTSVKSSP"
     gene            1446..2891
                     /locus_tag="VFF72_04170"
     CDS             1446..2891
                     /locus_tag="VFF72_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZV92386.1"
                     /translation="MRRGGSRAGVAFVAAAWLAGCTAGPDYVRPSVEMPATWKLEAPW
                     RAAAPDDAAPKGAWWRRFGDARLDALQEQALERSPTLAIAAARLEQARAVVRANAASL
                     LPSASLGVRDVRARISPNRPLSNYASPNFATVQNDFLPALSVSYEIDLAGRIRRGVEG
                     AQASAEQSAADLENARLLLAADLATAYFNLRATDIELDVLARSIALQRRSLDFVGTRH
                     DLGAASGLDVAQQQALLDTTLTQVDVLKRQRSGYEHAIATLVGTPAPSFSLAADTSEL
                     EPPAVPLGVPSDVLQRRPDVASAERAMAAANAQIGVARAAYYPSIVLSPAIGFESRSL
                     STLFEAPSILWSLGAQLMQPLFNAGRLDANVAFASAGYEATVGNYRRVVLTAMQEVED
                     GITGLADLGHANAQARTAVATARHVLDLANARYEGGATTYLDVITAQQSLLSAERLSA
                     QLGGQRLLVAVFLVKALGGGWQAPGGAIATR"
     gene            2932..3354
                     /locus_tag="VFF72_04175"
     CDS             2932..3354
                     /locus_tag="VFF72_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92387.1"
                     /translation="MPAGTTRLNVDVAASLAVQCLSREAEDNAMNASRTESLQSRLEN
                     VIALSGLLERVENTAISVDADQYRSLIRQLKSALSVPIPAPALDAILGAHPAVAELYE
                     NMNYETSGLSRISLDRSVATEMLASEVIARASRSARAG"
     gene            complement(3532..4167)
                     /locus_tag="VFF72_04180"
     CDS             complement(3532..4167)
                     /locus_tag="VFF72_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZV92388.1"
                     /translation="MPDPSRQPPRHFSMLRGFHLADFFTLANAACGVAGVLMAMQALV
                     SGRARDLLIAAAFAPAAFVFDVLDGRIARWRHTSSPLGRELDSLADVISFGVAPAALA
                     NACGMSGGWDAVVLVYFVCCGVSRLARYNVTAESLSGDSGKVAYFEGTPIPTSVLLTA
                     ILAIAAWQGRTGDALWGGAFRFADATLHPLVLMFALSGTLMVSKTLRIPKL"
BASE COUNT          619 a         1542 c         1470 g          585 t
ORIGIN      
        1 cgaacagaac gcgccgctcg gccgcgccgt catcggcggc cttgtcttcg cgacggtctc
       61 gacgctgctc ttcgtgcctg tcgtctacgc cgccatccac gatcgcctcg cccgccgcgc
      121 cgaacgacac gccgcacgcc atccggccgg cggtactgcc ctcctcgaag gttgaagccg
      181 atgtccgatc ctctcgccgc gccgccgtcc ggggccgtgc ctcccgtcga gggtgggacg
      241 ccttcgatcg ccgccccgcc gtccggtgcc gccgagcggg cccgggccgg acgaatcgcc
      301 cgcatcgtcg tcgccgtcgt cgtcgtgctg ctcatcgtcg gcgccggccg cacggtgatc
      361 gccaggatcg cgaactcgcg cgtgctcgag gcggacgcgg cggcacagtc ggtgcagtac
      421 gttcgcacca ccttcgcgaa agacagcggc gcggcgcaga ccctggcgct gcccggaacc
      481 ctgcagggct ggcagcaggc gccgatcgcg gcgcgcgcca ccggctacgt gaagcgatgg
      541 acgcacgaca tcggcagcca cgtcgccaag ggcgagctgc tggcggagat cgaatcgccc
      601 gaggtcgacc agcagctctc gcaggcggtc gctgcacgac agcagatgac ggcgagccag
      661 ggcctggcgc gaagcacggc ggcgcgctgg gaggcgctgc gcaagaagga cgtcgtctcg
      721 cagcaggagc tcgacgaaaa gcgcagcgcc gtcgcgcagg ccgacgccaa cctcgccgcc
      781 gcgaacgcca acgtgcagcg gctgcgccag atgcaggcgt tcacgcgcgt cacggcgccc
      841 tttgccggcg tcatcaccag gcgcagcgtc gataccggcg acctcgtcga ttcggccggc
      901 agggtgctgt tcgtgctgac ccagatggat ccgctgcgcg tctacgtcaa cgtgccgcag
      961 tcgtatgcgc agctcgtcag gcgcggccag aaggtcgtcg tgacgcagcc cgagttgcgc
     1021 ggccgcagct ttgcgggcga ggtggcgcgc acggccgact cgatcgacgc ggcgtcgcgc
     1081 acgatgcagg tcgagatcaa cctgcacaac ggcgacggcg cgcttcttcc cggcgcctac
     1141 gtgcaggtgg cgctgcccct ggcggccagc gcgaccctgg tcgtgccgac caacgtgctg
     1201 ctgtttcgcg gcgacggcac gcgagtcgcc gtcgtcgatg gcgccggaca cgtccgtctg
     1261 cggccggtga cgctcgggcg caacctcggc caggagatcg aggttctcga cggcatcggc
     1321 cccgccgacc ggctggtcgt caacccgtcg gactcgctcg ccgacggcga tacggtgcag
     1381 gtggccgcgg ccgatccggc gcaggccgcg agcggcggcg cgccgacgtc ggtgaagagc
     1441 tcgccgtgag gcgtggcggt tcgcgcgccg gcgtcgcctt cgtggcggcg gcctggctcg
     1501 ccggctgcac cgccggaccc gactatgtgc gcccctcggt cgagatgcct gccacctgga
     1561 agctcgaggc gccgtggcgt gccgccgcac ccgacgacgc cgcccccaag ggcgcgtggt
     1621 ggcgccgctt cggcgacgcc aggctcgacg cgttgcagga gcaggcgctc gaaagaagcc
     1681 cgacgctggc catcgccgcg gcccgcctcg agcaggcacg cgccgtcgtg cgcgccaacg
     1741 ccgcgtcgct gcttccgagc gccagcctcg gcgtgcgcga cgtccgcgcc cgcatctcgc
     1801 cgaaccggcc actcagcaac tacgcgtcgc ccaacttcgc gacggtgcag aacgatttcc
     1861 tgcccgcgct ctcggtcagc tacgagatcg acctggccgg ccgcatccgg cgcggcgtcg
     1921 aaggcgcgca ggcgagcgcc gagcaatcgg cggccgacct ggaaaacgcg cggctgttgc
     1981 tcgcggccga cctggcgacg gcctacttca acctgcgcgc gaccgacatc gagctcgatg
     2041 tcctcgcccg ttcgatcgcc ttgcagcgtc gctcgctcga cttcgtcggt acgcgccacg
     2101 acctgggcgc cgcgtccggg ctcgacgtcg cgcagcagca ggcgttgctc gatacgacgc
     2161 tgacgcaggt cgacgtgctc aagcgccagc gcagcggcta cgagcacgcg atcgcgaccc
     2221 tggtcgggac gccggcgcct tcgttctcgc tggccgccga cacgtccgag ctcgagccgc
     2281 cggccgtgcc gctcggcgtg ccctccgacg tgctgcagcg ccgcccggac gtcgcctcgg
     2341 cggagcgggc catggcggcg gcgaacgcgc agatcggcgt ggcccgcgcc gcctactacc
     2401 cgagcatcgt cctgtcgccg gccatcggct tcgagagccg cagcctgtcg acgcttttcg
     2461 aagcgccgag catcctctgg tcgctcggtg cgcaactgat gcagccgctc ttcaacgccg
     2521 gccgtctcga tgccaacgtc gccttcgcga gcgccggcta cgaggccacc gtcggcaact
     2581 accggcgcgt cgtcctcacg gcgatgcagg aagtcgaaga cggcatcacc gggctggccg
     2641 atctcgggca cgcgaatgcc caggcgcgga ccgcggtcgc gacggcgcgt cacgtcctcg
     2701 acctcgcgaa cgcccgctac gaaggcggcg cgacgaccta cctcgacgtc atcacggccc
     2761 agcagtcgct gctctcggcg gagaggttgt cggcgcagct cggcggacag cgcctcctcg
     2821 tcgcggtgtt cctcgtcaag gcgctgggcg gcggctggca ggcgccgggg ggcgcgatcg
     2881 cgacgcgctg acggggcgtc gtccggcagc gcggcggcac gccgtgcact cgtgccggcg
     2941 ggcacgacgc gcttgaatgt ggatgtcgca gcctcacttg ctgttcaatg cctctcccgg
     3001 gaggccgagg acaatgccat gaatgcttcc cgaaccgaat cgctgcagtc gcggctggaa
     3061 aacgtcatcg ccctgtcggg cctgctcgag cgcgtcgaga acaccgcgat cagtgtcgat
     3121 gccgatcaat accgcagcct gatccgtcag ctgaagtcgg cgctgtcggt gccgattccg
     3181 gcgcctgctc tcgatgccat cctcggcgcg cacccggccg tcgccgagct ctacgaaaac
     3241 atgaactacg agacttccgg gctgtcgcgc atctcgctcg accgctcggt cgccaccgag
     3301 atgcttgcat cggaggtgat cgcgcgcgcc tcgcgctcgg cgcgggcggg ctgaacgcaa
     3361 gggtcgtccc cacgacaggc gaaaggccgc ccttcgatgc cggcgggtcg acgtttcctc
     3421 ctgtgcgcga ggcaaagccc gaccgccgct gccccaggcc agccgcagtc cgcagacgaa
     3481 tggcacgcgt ctgttgcgcc ccgggcgggc gcacgccggg ctgtcgcgtc cctagagttt
     3541 cggaattcgc agcgtcttgc tcaccatcag cgttcccgac agggcgaaca tcagcacgag
     3601 cgggtgcagc gtcgcgtcgg cgaagcggaa ggcccctccc cacaaggcat cgcccgtccg
     3661 gccctgccag gcggcgatgg cgaggattgc ggtgaggagg acactggtcg gaatcggcgt
     3721 cccttcgaaa taggcgacct tgccgctgtc gcccgacagg ctttcggcgg tgacgttgta
     3781 gcgggccagc cgcgacacgc cgcagcagac gaaatacacg agcacgaccg cgtcccagcc
     3841 gccactcatg ccgcaggcgt tggcgagggc ggcgggtgcg accccgaagg agatgacgtc
     3901 ggccagcgaa tcgagctcgc ggccgagcgg cgacgacgtg tgtcgccagc gggcaatgcg
     3961 cccgtcgagg acgtcgaaga cgaacgccgc cggcgcgaag gcggcggcga tgagaaggtc
     4021 gcgcgcgcgc cccgacacca gcgcctgcat cgccatcagc acgccggcca ccccgcaggc
     4081 cgcgttggcc agagtgaaga agtcggcgag atgaaagccg cgcagcatcg agaaatggcg
     4141 gggcggctgg cgcgatggat cgggcatcgg gcgagtgttc cgcaagcctc gcccgttgtc
     4201 tgccagcgat ttccca
//
LOCUS       DASRWS010000226         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_950650, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000226 DASRWS010000000
VERSION     DASRWS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950650"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..2838)
                     /locus_tag="VFF72_04185"
     CDS             complement(214..2838)
                     /locus_tag="VFF72_04185"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HZV92389.1"
                     /translation="MPKSLIIAEKPSVAQDIVRALTPAAGKFDKHDEYFESEHYLVTS
                     AVGHLLEIKAPEEFDVKRGKWSFANLPVIPPHFDLAPLDKTKGRLNAIVKLVKRKDVA
                     ELINACDAGREGELIFRLIQQHAKSKQPVKRLWLQSMTPQAIRDGFEHLRSDKELRGL
                     AEAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIMVEREEQIRKH
                     VSRDYWEVRATFAVEAGEYEGKWFDPAWKKSPAVASGQEAAPVDNELRADRLWSEADA
                     LAIADAVRGEPASVTEEAKPSTQAPPLLYDLTSLQREANSRFGFSARTTLSIAQALYE
                     KHKVLTYPRNDSRALPEDYVAVARQTMEMISKEDLPGPLRPLAAHAKAALKAGYVKPS
                     KRIFDNAKVSDHFAIIPTLQAPRSLSEIEAKLYDMVAKRFLAVFFPSAEFMVTTRIST
                     VAKAGKEHKFQTNGKVMVKPGWLAVYGREAQEDDANLVAVGKGELARTEGVDVKALKT
                     RPPARYTEATLLSAMEGAGKLIDDDELREAMQEKGLGTPATRAAIIEGLIYEKYIHRE
                     GRELVPGAKAFQLMTLLRGLGVEDLTKPELTGNWEYQLAEMEKGQLDRDTFMKSIAAM
                     AERIVKKAKEYDRDTIPGDYATLAAPCPNCGGVVRENYRRYACTGVPAGAKAAAREPC
                     GFSISKIPGGRAFELAEVEAFLRDKRIGPLEGFRSKAGWPFTAELKLVFDDELANWKL
                     EFDFGEDAKREGESGEPVDFGDQEPLGHCPKTQGRVYEFGANYVCEHAVGANVTCDFK
                     SGKVILQQPVAREQMTKLLATGATDLLEGFVSNKTRRKFKARLAWDAKEGKVVFAFEP
                     RPPRAAKKAPAKAAKKAA"
     gene            2961..3452
                     /locus_tag="VFF72_04190"
     CDS             2961..3452
                     /locus_tag="VFF72_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZV92390.1"
                     /translation="MPRKTLSSPAESPKTLPRKAIEVRRSGIHGKGVFAAMPIAAGQR
                     IVEYTGPRITWKQAQAQHPHNPDDPNHTFFFHIDDKRVIDGLHGGPAKWINHACDPSC
                     EADEDDGRVFIKALRDIAVGEELNYDYGLVLEGRHTAKIKKEYECRCGSRKCRGTMLS
                     PKR"
     gene            3484..>4305
                     /locus_tag="VFF72_04195"
     CDS             3484..>4305
                     /locus_tag="VFF72_04195"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZV92391.1"
                     /translation="MSDAASMLEMLRAALGSRADQLDIVHVPSTGSTNADLLEKAREA
                     GAAMRAQLRVADRQTAGRGRLGRPWHGEPGASLAFSLAWPTRATTLAGLSLAIGVAAA
                     DALDPLPARRLRIGLKWPNDLWIVGDDEAAGRKLGGILVETTPAPGGRIVVVGVGLNI
                     LAQGVPGASSGVGWLREIDALATPSSALAKVAPALLDALARFDSEGFSPFAGRFATRD
                     LLCGRAVRTQAPTGGLPLEGVACGISAEGELLLQTAQGMVGIASGEVSVRPIVGES"
BASE COUNT          619 a         1508 c         1464 g          714 t
ORIGIN      
        1 atgcggccag cggcagcgcc gccgatgcga cggcgacgac gaccagccgg cgaaggaagg
       61 gggcggcacg ggtcacggac gttctcctcg atgtcgagga agagtctcgg gccgccgacg
      121 tcggccctcc gtcggacggc gccgcatcgg cagcgcggcc gaggcgccat cgcggcgccg
      181 gcctgcatcg cccggccgtc ggccacggcg ccgtcaggcc gctttcttcg cggctttcgc
      241 cggcgccttc ttggccgccc gcggcggccg cggttcgaag gcgaacacca ccttgccctc
      301 cttcgcgtcc caggccaggc gcgccttgaa cttgcgccgc gtcttgttgg agacgaagcc
      361 ttcgagcagg tcggtcgcgc cggtggcgag cagcttggtc atctgctcgc gcgccaccgg
      421 ctgctgcagg atgactttgc cgctcttgaa gtcgcaggtg acgttggcgc cgacagcgtg
      481 ctcgcagacg tagttggcgc cgaactcgta gaccctgccc tgcgtcttcg gacagtggcc
      541 gagcggctcc tggtcgccga agtcgaccgg ctcgcccgac tcgccctcgc gcttggcgtc
      601 ctcgccgaaa tcgaactcga gcttccagtt ggcgagctcg tcgtcgaaga cgagcttgag
      661 ctcggcggtg aacggccagc cggccttgga ccggaaaccc tcgagcggac cgatccgctt
      721 gtcgcgcagg aacgcctcga cctcggcgag ctcgaaggcg cgaccgcccg ggatcttgct
      781 gatcgaaaag ccgcacggct cgcgggccgc cgccttggcg ccggccggga cgccggtgca
      841 ggcgtagcga cgatagttct ccctgacgac gccgccgcag ttcggacacg gcgccgccag
      901 cgtcgcgtag tcgccgggga tggtgtcgcg gtcgtactcc ttggccttct tgacgatgcg
      961 ctcggccatc gccgcgatgc tcttcatgaa ggtgtcgcga tcgagctggc ccttttccat
     1021 ctcggccagc tggtactccc agttgccggt gagctcgggc ttggtcaggt cctcgacgcc
     1081 caggccgcgc agcagcgtca tcagctggaa cgccttggcg ccgggaacga gctcgcggcc
     1141 ttcgcgatgg atgtacttct cgtagatgag cccttcgatg atcgcggcgc gggtcgccgg
     1201 cgtgcccagg cccttttcct gcatcgcctc gcgcagctcg tcgtcgtcga tcagcttgcc
     1261 ggcgccttcc atcgccgaga gcagcgtcgc ttcggtgtag cgggccggcg gccgggtctt
     1321 cagggccttg acgtcgacgc cttcggtgcg cgccagctcg cccttgccga cggcgacgag
     1381 gttggcgtcg tcttcctgcg cctcgcggcc gtagaccgcg agccagccgg gcttgaccat
     1441 caccttgcca ttggtctgga acttgtgctc cttgccggcc ttggcgacgg tcgagatccg
     1501 cgtcgtcacc atgaattcgg ccgacggaaa gaacacggca agaaagcgct tggcgaccat
     1561 gtcgtagagc ttggcctcga tctccgagag gctcctgggc gcctgcaggg tcgggatgat
     1621 cgcgaagtgg tcggaaacct tcgcgttgtc gaagatccgc ttcgacggct tgacgtagcc
     1681 cgccttcagc gccgccttcg catgcgcggc caggggccgc agcggcccgg gcaggtcttc
     1741 cttcgagatc atctccatcg tctgccgggc gacggcgacg tagtcctcgg gcagggcgcg
     1801 gctgtcgttc cgcggatagg tgaggacctt gtgcttctcg tagagcgcct gggcgatcga
     1861 cagcgtcgtc cgcgccgaaa agccgaaacg cgagttggcc tcgcgctgca ggctggtcag
     1921 gtcgtagagg aggggcggcg cctgcgtgct cggcttggcc tcctcggtga ccgaggccgg
     1981 ctcgccccgc accgcgtcgg cgatggcgag cgcgtcggcc tcgctccaca ggcgatcggc
     2041 gcgcagctcg ttgtccaccg gagccgcctc ctggccggag gcgacggccg gactcttctt
     2101 ccaggcgggg tcgaaccact tgccctcgta ttcgccggcc tcgacggcga aagtcgcgcg
     2161 cacctcccag tagtcgcgcg agacgtgctt cctgatctgc tcctcgcgct cgaccatgat
     2221 cgacagcgtc ggcgtctgca cccggccgac ggtggtgagg aagaagccgc cgtcgcgcga
     2281 gttgaacgcc gtcatcgccc gcgtgccgtt gatgccgacc agccagtcgg cctcggagcg
     2341 gctgcgcgct gcctcggcca ggccgcgcag ctccttgtcg ctgcgcaggt gctcgaagcc
     2401 gtcgcggatc gcctgcggcg tcatgctctg cagccacagc cgcttgaccg gctgcttcga
     2461 cttcgcgtgc tgctggatca ggcgaaagat gagctcgccc tcgcgtccgg cgtcgcaggc
     2521 attgatcagc tcggcgacgt cctttcgctt gacgagcttg acgatggcat tgaggcggcc
     2581 cttggtcttg tcgagcggcg ccaggtcgaa gtgcggcggg atgaccggca ggttggcgaa
     2641 gctccacttg ccgcgcttga cgtcgaactc ctcgggcgcc ttgatctcga gcaggtgacc
     2701 gacggcggag gtgacgaggt agtgctcgct ctcgaaatac tcgtcgtgct tgtcgaactt
     2761 tcccgccgcg ggagtgaggg cgcgcacgat gtcctgtgcg acggaaggct tctcggcaat
     2821 gatcagtgac ttgggcatct gtggttcgag actcggcaag aggttcgcga tgcgcccggg
     2881 aacgcatcca tacaatccgg gctcctcgcg cgcacgcaca cacgcgcgcg cccatgaatt
     2941 caatgtaccc aaagaagccg atgccccgaa aaaccctgtc gagccctgcc gaatcgccga
     3001 agacgctgcc tcgcaaggcg atcgaggtgc ggcgttcggg aatccacggc aagggcgtgt
     3061 tcgcggccat gccgatcgcg gcggggcagc gcatcgtcga gtacaccggg ccgcgcatca
     3121 cctggaagca ggcgcaggca cagcatccgc acaaccccga cgatccgaac cacaccttct
     3181 tcttccacat cgacgacaag cgcgtgatcg acggcctgca cggcggcccg gcgaagtgga
     3241 tcaaccacgc ctgcgatccg agctgcgagg ccgacgagga cgacggccgc gtcttcatca
     3301 aggcgctgcg cgacatcgcc gtgggcgagg agctcaacta cgactacggc ctcgtcctcg
     3361 agggccggca cacggcgaag atcaagaagg aatacgaatg ccgctgcggc agccgcaaat
     3421 gccgcggcac gatgctctcg cccaagcgct gatggccatt tcgcccacgg cccggcctgc
     3481 accgtgagcg acgcggcgtc gatgctcgag atgcttcgcg ctgcgctcgg cagccgtgcc
     3541 gatcaactgg acatcgttca cgtcccgagc accggctcga ccaacgccga tctcctcgag
     3601 aaggcccgcg aggccggtgc cgcgatgcgc gcgcagctgc gggtcgccga tcggcagacg
     3661 gccggccgcg gccgtctcgg ccggccctgg cacggcgaac ccggtgcctc gctggccttc
     3721 tcgctcgcct ggccgacacg tgcgacgacc ctggccggcc tgtcgctggc gatcggcgtc
     3781 gccgccgccg acgcgctcga tcccttgccg gcccgacggc tgcgcatcgg cctcaagtgg
     3841 cccaacgacc tctggatcgt cggcgacgac gaggccgcgg gtcgcaagct cggcggcatc
     3901 ctcgtcgaga cgaccccggc gccaggcggc cgcatcgtcg tcgtcggcgt cggtctcaac
     3961 atcctcgcgc aaggcgttcc cggcgcatcg agcggcgtcg gctggctgcg cgagatcgac
     4021 gccctggcca cgccgtcgtc ggcgctcgca aaggtcgcgc cggcgctgct cgatgccctc
     4081 gcgcgattcg acagcgaagg cttcagccct ttcgccggcc gcttcgcgac gcgcgatctc
     4141 ctttgcggac gcgccgtccg cacccaggcg ccgacaggcg gcctgccgct cgagggcgtg
     4201 gcctgcggca tctcggccga gggcgagctg ctgctgcaga ccgcgcaggg catggtcggt
     4261 atcgccagcg gcgaagtcag cgtccgaccg atcgttggcg aatcg
//
LOCUS       DASRWS010000227         5204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_665497, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000227 DASRWS010000000
VERSION     DASRWS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665497"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFF72_04200"
     CDS             <1..429
                     /locus_tag="VFF72_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HZV92392.1"
                     /translation="ALKQAEAGVYDVERIAAFTENHRRSGGHGSLSEHYTAAYGRAAF
                     DKSLKKHIVFSDHSLATDSVFSEVQLVSCRNVLIYFDRALQDRALGLFADSLCRQGFL
                     GLGAKESLRFSAQAGVFDELVPGERIYRKHRNAPAAKAAA"
     gene            426..1061
                     /locus_tag="VFF72_04205"
     CDS             426..1061
                     /locus_tag="VFF72_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZV92393.1"
                     /translation="MSDVASLAGTPDAVAIGASAGGVEALSVLLPALPAGLAMPVFVV
                     LHLPRERPSLLVDIFQPKCALPVREAEDKEPVEPGVVYIAPPDYHLLVDAGPRIALSA
                     DEPVHFSRPSIDVLFESAADIYADRLLAIVLTGANEDGAAGAAAVERAGGKVLVQDPD
                     SAQASLMPASTIKRVGTAVVRDLAGLAALLASLGLPSHGTRRGAHARRAQR"
     gene            1058..2188
                     /locus_tag="VFF72_04210"
     CDS             1058..2188
                     /locus_tag="VFF72_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZV92394.1"
                     /translation="MSQSPVVNCLIVDDLEDNLIALEALLAEPGLRILRARSGAEALE
                     LLLVHPVALALLDVQMPEIDGFELAELMRGSERTRHIPIIFVTAGLGDQRRVFKGYES
                     GAVDFLLKPIEPQVLKSKARVFFQLHRQSLQIAEELRERTETLRMNELFTAMLGHDLR
                     GPLSAIVMASMIIERKSTDEGLRKMATRSLSSARSMSRMIEDMLDLARARIGGGIVVR
                     RVRGDLAAPMHRVADECAAAHPQGRLEWRVDGDCTGEWDVDRLAQVASNLIGNAFRHG
                     TPGEPVLVRLDGRADDEVVFSVENGGRIAAEVLPRLFDPFRGREEPSSRGEGLGIGLY
                     IVRQIVTAHGGRVDASSNDGERTVFTVRLPRRGEAAAAADAR"
     gene            2248..2883
                     /locus_tag="VFF72_04215"
     CDS             2248..2883
                     /locus_tag="VFF72_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92395.1"
                     /translation="MTTRRDKKQTGRILVVEDDWDILEVLKLMLEDEGHHVVTAKHGR
                     AALAAAAAKSFDLVVMDISMPEMSGIDVARELRADPKTADVYIVVHTGLDEHWVRERF
                     ADYDLFLTKAQDADVLVERIAGLLAQPRAERRQAAAEPARFSADDLMRAQHALRQGMG
                     LGNETYPLDTFLGLLGEDIAQLRRLERSDADIAELISGALGRPITAGDLPN"
     gene            2929..3498
                     /locus_tag="VFF72_04220"
     CDS             2929..3498
                     /locus_tag="VFF72_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92396.1"
                     /translation="MRAKARRVDSRERARRWLLTGVVLLVAGCATPAPPAPSSAQQQV
                     DAAEALAVERHWLQSWFRSTPVRIAQRASGAIDIEVPREFCFETGRSVVEPALEAVLD
                     KLAQSLGRVPAAQVSLIAAPGDGPVTTSAPGAATTIATGLALQRARAVREHLRDAGID
                     ASRLGPPSAAAAPSVHLYITATAAKLRKP"
     gene            complement(3625..4977)
                     /locus_tag="VFF72_04225"
     CDS             complement(3625..4977)
                     /locus_tag="VFF72_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZV92397.1"
                     /translation="MNAATLFRAIASMDGAPQARVSMPEVHRSIAVPLPGTGRSWRRW
                     LSFFGPGVLVAVGYMDPGNWATSLAGGSQFGFSLLSVILLSNFLAMLFQAAAVRLGIG
                     AGMDLAQACRKHFSARTSLLLWLFCEVAIVACNLAEVIGMAIGINLLFGLPLLVGVGL
                     TFVDVMLILALQTRGFRYLEAVVIGLVAAIAVCFAFQLFWLHPAMGDVLLGFVPRPEI
                     VANPQMLYLAVGIVGATVMPHNLYLHSAIVQTRRHEETARGKRDAIRFATIDSNLALG
                     LALFVNAAIMIVASGAFHRGGIGPVSDLGDAYKLLSPLLGVGVASALFGIALLCSGLS
                     SSVTGTLAGQIVMDGFLHLRVPAAWRAMLTRSVAIVPAIAVIAYYGSAGANSLLVFSQ
                     VVLSLQLPFAIVPLLMFTTSRKHLGEFAFGRATSVLMWAGAATIAILNVWMLQLLVTG
                     "
BASE COUNT          846 a         1848 c         1787 g          723 t
ORIGIN      
        1 gcgctgaagc aggccgaagc gggggtctac gatgtcgagc ggatcgcggc cttcaccgag
       61 aaccaccggc gctccggcgg ccacgggtcg ctgtccgagc actacaccgc cgcgtacggc
      121 cgcgccgcct tcgacaagtc cctgaagaag cacatcgtct tttccgacca cagcctggcc
      181 accgacagcg tcttttccga agtgcagctc gtctcgtgtc gcaacgtcct catctacttc
      241 gaccgggccc tgcaggaccg cgccctgggc ctgttcgccg actcgctgtg tcgccagggc
      301 ttcctcggcc tgggcgccaa ggagtcgctg agattctcgg cgcaggccgg cgtcttcgac
      361 gagctggtgc ccggcgagcg catctaccgc aagcaccgca acgcgcctgc cgcgaaggct
      421 gcggcatgag cgacgtcgcc agcctcgccg gaacgcccga tgccgtcgcc atcggcgcgt
      481 cggcgggggg cgtcgaggcc ctgtcggtgc tgctgccggc cctgcccgca gggctggcga
      541 tgccggtctt cgtcgtgctg cacctgccgc gcgagcggcc gagcctgctg gtcgacatct
      601 tccagccgaa gtgcgcgttg ccggtgcgcg aggccgagga caaggagccg gtcgagcccg
      661 gcgtcgtcta catcgcgccg cccgactacc acctgctcgt cgatgcggga ccgaggatcg
      721 ccctgtcggc cgacgagccg gtgcatttct cgcgaccgtc gatcgacgtg ctgttcgaat
      781 cggcggccga catctacgca gaccgcctgc tcgcgatcgt gctgacgggg gccaacgagg
      841 acggcgcggc cggtgccgcc gccgtggagc gggccggcgg caaggtgctt gtccaggatc
      901 ccgacagcgc ccaggcgtcg ctgatgccgg cctcgacgat caagcgcgtc ggcacggccg
      961 tcgttcgcga tctcgccgga ctggccgcgc tgctcgcttc gctcggcctg ccgagccacg
     1021 gcacgcgccg cggcgcccac gcaaggagag cgcagcgatg agccagtcgc ccgtcgtcaa
     1081 ctgcctcatc gtcgacgatc tcgaggacaa cctgatcgcc ctcgaggcgc ttctcgccga
     1141 gccgggactg cgcatcctgc gggcgcgttc gggcgccgag gcgctcgagc tgctgctcgt
     1201 ccacccggtg gcgctggccc tcctcgacgt gcagatgccc gagatcgacg gcttcgagct
     1261 ggccgagctg atgcgcggca gcgagcgcac gcgccacatc ccgatcatct tcgtcaccgc
     1321 cgggctcggc gaccagcgcc gcgtcttcaa gggctacgag agcggtgcgg tcgacttcct
     1381 cctgaagccg atcgagccgc aggtcctcaa gagcaaggcc cgcgtctttt tccagttgca
     1441 ccggcaaagc ctgcagatcg ccgaggagct gcgcgagcgc accgagacgc tgcgcatgaa
     1501 cgagctgttc acggcgatgc tcggccacga cctgcgcggc ccgttgagcg ccatcgtcat
     1561 ggcctcgatg atcatcgagc gaaagtccac cgacgagggc ctgcgcaaga tggcgacccg
     1621 gtcgctgtcg agcgcgcgca gcatgagccg gatgatcgag gacatgctcg acctggcgcg
     1681 tgcccgcatc ggcggaggca tcgtcgtgcg ccgggttcgc ggcgatctcg ccgcgccgat
     1741 gcaccgcgtc gccgacgagt gcgcggccgc ccatccgcaa ggccgcctcg aatggcgggt
     1801 cgacggcgac tgcacaggcg aatgggacgt cgaccggctc gcccaggtcg cctccaacct
     1861 gatcggcaac gccttcaggc acggcacgcc tggcgagccg gtcctcgtgc gcctcgatgg
     1921 tcgcgctgac gatgaggtcg tcttctcggt cgagaacggc ggccgcatcg ccgccgaggt
     1981 gttgccgcgc ctgttcgatc cgtttcgcgg ccgcgaggag ccgtcgtcgc gcggcgaagg
     2041 gctcgggatc ggcctgtaca tcgtccgcca gatcgtcacc gcccacgggg gccgcgtcga
     2101 cgcgagctcg aacgacggcg agcgcacggt gttcaccgtc cgcctgccgc gccgcggcga
     2161 ggcggccgca gcggccgacg ctcgctgaag cgcggccacg acccgaccag caacccgagg
     2221 cgccccgatg tctgccttct gcccgccatg acgacccgcc gcgacaagaa acaaaccggt
     2281 cgcatcctcg tcgtcgagga cgactgggac atcctcgagg ttctcaagct gatgctcgag
     2341 gacgaaggac accacgtcgt caccgcgaag cacggccggg ccgcgctcgc cgcggccgcc
     2401 gccaagtcgt tcgatctggt cgtcatggac atctcgatgc ccgagatgag cggcatcgat
     2461 gtcgcccgcg agctgcgcgc cgatccgaag accgccgacg tctacatcgt cgtccacacc
     2521 ggtctcgacg agcattgggt ccgcgagcgg ttcgccgact acgacctctt cctcaccaag
     2581 gcccaggacg ccgacgtgct ggtggaacgc atcgccggct tgctggcgca gccgcgcgcc
     2641 gaacgtcgcc aggccgctgc cgagccggcg cgcttcagcg ccgacgatct gatgcgggca
     2701 cagcatgcgc tgcgccaggg catgggcctc ggcaacgaga cctatccgct cgacacgttt
     2761 ctcggcctgc tcggcgagga catcgcgcaa ttgcgacggc tcgagcgcag cgacgccgac
     2821 attgccgagc tgatttccgg cgcgctcggc cggccgatca ctgccggcga cctgccgaac
     2881 tgacggatga gcgaccggac ggtcccgctc gggatgcgag agagcatcat gagggcgaag
     2941 gctcgccgcg tcgattcccg cgagcgagcc cgccggtggc tcctgaccgg cgtcgtcttg
     3001 ctggtggccg gatgcgcgac cccggcgccg cctgcaccgt cgagcgccca gcagcaggtc
     3061 gatgcggccg aggcgctcgc ggtagagcgc cactggctcc agtcgtggtt caggtcgacg
     3121 ccggtgcgga tcgcccagcg cgcaagcggc gccatcgata tcgaggtgcc gcgcgaattc
     3181 tgtttcgaga ccggacgcag cgtcgtcgag ccggcgctgg aggccgtgct cgacaagctc
     3241 gcgcaaagcc tcggccgcgt gcctgcagcc caggtgtcgc tgatcgccgc gcccggcgac
     3301 ggcccggtga cgacgagcgc ccccggcgcg gcgacgacga tcgcgaccgg cctggcgctg
     3361 cagcgggcac gggcggtgcg cgagcatctg cgggatgccg gcatcgacgc cagccgcctc
     3421 gggccgccga gcgccgctgc ggctccgtcg gttcatctct acatcacggc gacggcagcg
     3481 aagcttcgca agccgtgaga agcgtcggcg gccgttccac ggggccgcgt ggtcgtcggg
     3541 aaggtcgcgg cgcgccgtcc ccgacgacga cgcgccgcac ccgcgccacg cccgccacga
     3601 ccgtcacgct gcgcgcgcac gcagtcagcc cgtcacgagg agctgcagca tccagacgtt
     3661 gaggatggcg atggtcgccg cacccgccca catcagcacg ctggtcgcgc ggccgaaggc
     3721 gaattcgccg agatgcttgc ggctcgtcgt gaacatcagc agcgggacga tcgcaaaagg
     3781 cagctgcagg ctgaggacca cctggctgaa gacgagcagc gagttcgcac ccgcacttcc
     3841 gtagtacgcg atcacggcga tcgccggaac gatcgccacc gatcgcgtca gcatggcccg
     3901 ccacgccgct ggcacgcgca gatgaaggaa tccgtccatg acgatctgcc cggccagcgt
     3961 ccctgtcacc gacgagctga gcccggagca cagaagggcg atgccgaaca gcgcgctcgc
     4021 gacgccgacg ccgagcagcg gcgagagcag cttgtaggcg tcgccgaggt cggagaccgg
     4081 cccgatcccg ccgcggtgaa acgctcccga ggcgacgatc atgatcgccg cgttgacgaa
     4141 gagggccagc ccgagcgcga ggttggaatc gatcgtcgcg aagcggatcg cgtcgcgctt
     4201 gccccgggcc gtctcttcgt ggcgccgcgt ctgcacgatg gccgagtgca ggtagaggtt
     4261 gtgcggcatc accgtcgcgc cgacgatgcc cacggccagg tagagcatct gcggattggc
     4321 gacgatctcg ggtcgcggca cgaagccgag caggacatcg cccatcgcgg gatgcagcca
     4381 gaacagctgg aaggcgaagc agacggcgat cgccgcgacc aggccgatca ccaccgcctc
     4441 gaggtagcga aagccgcgcg tctgcagcgc caggatcagc atcacgtcga cgaacgtgag
     4501 gcccacgccg acgagcaacg gcagcccgaa caacaggttg atgccgatcg ccatgccaat
     4561 cacctcggcg aggttgcagg cgacgatggc cacctcgcag aacaaccaga gcaggaggct
     4621 ggtgcgcgcc gagaaatgct tgcggcaggc ttgcgcgaga tccattccgg cgccgatgcc
     4681 cagacgaacc gcggcggcct ggaacagcat cgccaggaaa ttggaaagaa ggatgaccga
     4741 gagcagcgaa aagccgaact gcgagccgcc cgccaaggag gtcgcccagt tgccaggatc
     4801 catgtagccc acggccacca gcacgcccgg gccgaagaac gacaaccatc gtcgccagct
     4861 gcgccccgtc cctggcaagg gcacggcgat cgaacggtgc acctcgggca tgctgacgcg
     4921 cgcctgcggc gcgccgtcca tcgacgcgat ggccctgaac agggtggcgg cattcatggt
     4981 cctgcttccc gtgcgaagtg aaacgcgggc acgtggcagg cccttcgcgc cgctggcgcg
     5041 aagggccgcg cctcgactat tccgcagtac tcatgaagat gtttccgttg accccggcag
     5101 ggaagaagcc gcccttggac gccgcggccg ccgtgaggta gacgatgttg agcacctgtc
     5161 cggtggtccg gctgtaggcg atgccgttgc tgtcgagcgg ggcg
//
LOCUS       DASRWS010000228         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4116337, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000228 DASRWS010000000
VERSION     DASRWS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4116337"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..1514
                     /locus_tag="VFF72_04230"
     CDS             429..1514
                     /locus_tag="VFF72_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92398.1"
                     /translation="MQYWLRHAADRDLDDVDWRNRPSVPQRTGRVPRAQEDRVLRARR
                     HLRERTALGEYGARAIHEFLRARHDDIPSVRTIGRILERRGALDVASRVRRPPPPPGW
                     YLHDVATREAELDSFDTVMGLVIANGPEVEVLNGISVHGGVVASWPTTGVRARDVVTF
                     LVEHWREVGLPRYAQFDNDTRFQGAHQFADAFGRVTRLCLSLGVVPVFAPPREPGFQG
                     AIEAYNGRWQAKVWARFHHTSLRALTRRSDAYVRAHRARNALRADAAPARRPFPARWS
                     FDVHGPLRGRVVYIRRTDGRGAVEVLGHRFSIDARWVHRLLRAEVHLDRGVIHFYALR
                     RRAPDSQPLLHTARYQPPLGAFRGRDP"
     gene            complement(1661..2008)
                     /locus_tag="VFF72_04235"
     CDS             complement(1661..2008)
                     /locus_tag="VFF72_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92399.1"
                     /translation="MRPAKFRGAVIEGHKGVVALVVPFDPRAVWELEPIALAGRRHGW
                     PVVGRIGRKRFTGYIGERWGRFFVMLDAAALGVRPGDTLSVSLEPTRDAEVIAAAVAQ
                     SRATTQPRKARKL"
     gene            complement(2011..>2552)
                     /locus_tag="VFF72_04240"
     CDS             complement(2011..>2552)
                     /locus_tag="VFF72_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92400.1"
                     /translation="LTQRYQAGGYADHGDKELVLAPGAHGLMIVREEMLYSAPGWTPD
                     PKAEFDASALASPITAIVKQDASDPHRVDDCGSVSYTLELTDAHKRKLTTDVGSGVIM
                     IDKPHATIAADPTADKLFDFGQWCAGGADYYQIVKNGDSLVVRYKAEDEGGDPDDPQP
                     APTWETRLTVKLAPGATIR"
BASE COUNT          356 a          890 c          881 g          425 t
ORIGIN      
        1 tcacctcgcc aagccgacgc tcgagtgcgg caacgcgctc gacgagatcc aggtcggcac
       61 gccggtgctc gtcgagggca aggtcacgag cggcaacccg acctggacca agcgcccgtt
      121 cgccggcccc gccctcgacg actgcaagct gatcacgccg tgatcaggcc tcggtcctcg
      181 ggcttcaggc ctcggccctg atccgagatg tcgcgattcg tctggctgag gcccgaggcc
      241 tgaggggcca ggtgtcagca cctcccggtc actccgagtg ccaccaccac ccggtcaccc
      301 cgggcccgaa ataggctgcg cggggtgcca agaagcgcgt agcgcggcgt cgcgccgcgg
      361 tggaagcagt tcgagctggc gagtcgcagc gctcagtcgc gcgacggttt cagctgccgt
      421 tgtcgaccgt gcagtactgg ctgcgacacg ctgcggatcg cgatctggac gacgttgact
      481 ggcgcaatcg gccgtccgtc ccacaacgca ccggacgtgt gccgcgagcg caggaggatc
      541 gcgtgttgcg agcgcggcgg cacttgcgcg agcgcacggc gctcggtgaa tacggagcgc
      601 gcgccattca cgagttcctg cgagcgcggc acgacgacat cccttccgtg cgcaccatcg
      661 gccgcatcct cgagcgccgc ggtgcactcg atgtggccag tcgtgtgcga cggccgccac
      721 cgccgcccgg ctggtatctg cacgacgtcg ccacgcgcga ggccgagctc gacagcttcg
      781 acacagtgat gggcctcgtc atcgcgaacg ggccggaggt cgaggtcttg aacggcatca
      841 gcgtgcacgg cggcgtggtc gcgtcgtggc cgacgaccgg cgtccgcgcg cgcgacgtgg
      901 tcacgtttct cgtcgagcat tggcgcgaag tcgggttgcc gcgctacgcg cagttcgaca
      961 acgacacgcg cttccagggc gcacatcagt tcgctgatgc gtttggtcgt gtcacgcgct
     1021 tgtgcctcag cttgggcgtc gtgcctgtgt tcgcgccgcc acgcgagccc ggcttccaag
     1081 gagccatcga ggcctacaac ggccgctggc aggcaaaggt ctgggcgcga ttccaccaca
     1141 cgtcgcttcg tgcactgacg agacgctccg atgcgtacgt acgcgcgcac cgcgctcgca
     1201 atgcgcttcg tgccgacgcc gcgccagcgc gacggccatt tcccgcgcga tggtccttcg
     1261 acgtgcacgg gccactccgc ggccgagtcg tctacatcag gcgaactgat ggtcgcggcg
     1321 ccgtcgaggt tctcggccat cgcttctcga tcgacgcacg ctgggtccac cgcctacttc
     1381 gtgctgaagt gcatctcgac cgcggcgtga tccatttcta tgccctgcga cggcgagcac
     1441 cggactcgca accactgctt cacactgccc gctaccagcc gccgctcggt gcttttcgag
     1501 gccgagatcc ctgaccggga ggtggtggca ctccagaggg gtcacccttc tacagggtga
     1561 ccggatggtg gtggcacttg gccgatcagg gctctggtac cggtgctgtc gctggcgctg
     1621 tcgctgccgc gctctcgtcg gccgcccgtc acgccaagcc ttagagcttg cgcgccttgc
     1681 gcggttgcgt cgtcgcgcgc gattgcgcga cggccgcggc gatgacctcg gcgtcgcggg
     1741 tcggctcgag cgagacggac agcgtgtcgc ccggtcggac gccgagcgcg gcggcgtcga
     1801 gcatgacgaa gaagcggccc cagcgctcgc cgatgtagcc ggtgaagcgc ttgcgcccga
     1861 tgcggccgac gacgggccag ccgtggcggc ggccggcgag cgcgatcggc tcgagctccc
     1921 agaccgcgcg cggatcgaac ggcacgacca gcgccaccac gcccttgtgg ccctcgatca
     1981 ccgcgccgcg gaacttcgcc gggcgcacgg ctacctgatc gtcgcgccgg gcgcgagctt
     2041 cacggtcagc cgcgtctccc aggtcggcgc cggctgtgga tcgtcgggat cgccgccttc
     2101 gtcctcggcc ttgtagcgca cgaccagcga gtcgccgttc ttcacgatct ggtagtagtc
     2161 ggcgccgccc gcgcaccact ggccgaagtc gaacagcttg tcggcggtcg gatcggcggc
     2221 gatcgtggcg tgcggcttgt cgatcatgat cacgccgctg ccgacgtcgg tggtgagctt
     2281 gcgcttgtgc gcgtcggtga gctcgagcgt gtagctgacg ctgccgcaat cgtcgacgcg
     2341 atgcggatcg ctcgcgtctt gcttcacgat cgcggtgatc ggcgaggcga gcgcgctggc
     2401 gtcgaattcg gctttcggat cgggcgtcca gccgggcgcg ctgtacagca tctcctcgcg
     2461 cacgatcatc aggccgtgcg cgccgggcgc gagcacgagc tccttgtcgc cgtgatcggc
     2521 gtaaccgccg gcctggtagc gctgggtcag ct
//
LOCUS       DASRWS010000229         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3538939, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000229 DASRWS010000000
VERSION     DASRWS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3538939"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1038
                     /locus_tag="VFF72_04245"
     CDS             55..1038
                     /locus_tag="VFF72_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZV92401.1"
                     /translation="MTSKTIKVALAGAGAFGTKHLDGIRNIDGVEVVSVIGRELGKTE
                     AVAKKYGIAHFGTDLSESLARPEVDAVILCTPTQMHAEQSIACLKAGKHVQVEIPLAD
                     SLEDAEAVAAMQKKTGLVAMVGHTRRFNPSHQWVHRRIAAGEFRIQQMDVQTYFFRRT
                     NTNALGQARSWTDHLLWHHAAHTVDLFAYQAGGEIVQAHAVQGPIHPVLGIAMDMSIQ
                     LKSSTGAICTLSLSFNNDGPLGTFFRYIGDTGTYLARYDDLFDGKDEKIDVSKVAVSM
                     NGIELQDREFFAAIREGREPNSSVAQVLPCYRTLHRLEQQLAAAGLGGRTP"
     gene            1035..2027
                     /locus_tag="VFF72_04250"
     CDS             1035..2027
                     /locus_tag="VFF72_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV92402.1"
                     /translation="MKQRRIGPLEVSAIGLGCMNLSHAYGAPPSPDVAEKVLLSALDL
                     GVTLFDTAALYGFGANETLLGRVLAPHRSKIVLASKCGMTGVNGVRVIDNRPETLRRS
                     CEESLQRLRTDVIDLYYLHRFDRKGPIEDSVGALADLVRAGKVRAIGLSEVSAATLRR
                     AHAVHPVSALQSEYSLWSRNPEIAGLEACREVGASFVAFSPIARGFLSGRLREVATLD
                     AQDIRRNMPRFQPAAFDANLSLLESFTALADEAGCTPAQLALAWLLAQDASLVPIPGT
                     TRVDHLAENVGAASVALEAGLLARVDATINRHTVKGPRYNATTQAEIDTEEFPA"
     gene            2024..>2315
                     /locus_tag="VFF72_04255"
     CDS             2024..>2315
                     /locus_tag="VFF72_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZV92403.1"
                     /translation="MRRRSMLTAVGGAALLRVAEAEATWPARPVTLVVPFAAGGIADL
                     TARTVTKAMATLLGQAIVVDNRPSAGSVVGSGMVAHATPDGYTLLLMSNANAI"
BASE COUNT          391 a          838 c          757 g          329 t
ORIGIN      
        1 tcacgtgccc gcgagcaaca cggccgtggg caacctgatc ctggagaacc cgcaatgact
       61 tcgaagacga tcaaggtggc actcgccggc gccggcgcgt tcggcaccaa gcacctcgac
      121 ggcatccgca acatcgacgg cgtcgaagtc gtctcggtga tcggccgcga gctcggcaag
      181 accgaggcgg tggcgaagaa gtacggcatc gcccatttcg gcaccgatct ctcggagagc
      241 ctcgcgcggc ccgaggtcga cgcggtgatc ctgtgcacgc cgacgcagat gcacgccgag
      301 cagagcatcg cctgcctgaa ggccggcaag cacgtgcagg tggagattcc gctcgccgac
      361 tcgctcgagg acgccgaggc cgtggccgcc atgcagaaga agaccggcct ggtcgcgatg
      421 gtcggccaca cgcgccgctt caacccgagc catcaatggg tgcaccggcg gatcgcggcg
      481 ggcgaattca ggatccagca gatggacgtg cagacctact tcttccggcg cacgaacacc
      541 aacgccctcg gccaggcgcg cagctggacc gaccacctgc tctggcacca cgcggcgcac
      601 accgtcgatc tgttcgcgta ccaggccggc ggcgagatcg tccaggccca tgccgtgcag
      661 gggccgatcc atccggtgct cggcatcgcc atggacatga gcatccagct gaaaagcagc
      721 accggcgcga tctgcacgct cagcctctcg ttcaacaacg acggcccgct cggcaccttc
      781 tttcgctaca tcggcgacac cggcacctac cttgcgcgct acgacgacct cttcgacggc
      841 aaggacgaga agatcgacgt ctcgaaggtc gcggtgtcga tgaacggcat cgagctgcag
      901 gaccgcgaat tctttgccgc gatccgcgaa ggccgcgagc ccaactcgag cgtcgcccag
      961 gtgctgccct gctaccgcac gctgcaccgg ctcgagcagc agctcgccgc ggcgggctta
     1021 ggcgggcgaa cgccatgaag cagcgccgca tcggaccgct cgaagtcagc gccatcggcc
     1081 tgggctgcat gaacctcagt cacgcctacg gcgcgccgcc ttcgcccgac gtcgccgaga
     1141 aggtgctgct gtccgcgctc gatctgggcg tgacgctctt cgataccgcc gcgctgtatg
     1201 gcttcggcgc caacgagacg ctgctcggcc gcgtcctcgc gccgcaccga tcgaaaatcg
     1261 tgctcgcgag caaatgcggc atgaccggcg tgaacggcgt gcgcgtcatc gacaaccggc
     1321 ccgaaacgct gcgtcgcagc tgcgaggaaa gcctgcagcg cctgcgcacc gacgtgatcg
     1381 acctctacta cctgcaccgc ttcgaccgca aggggccgat cgaggacagc gtcggcgccc
     1441 tggccgacct cgtgcgcgcc ggcaaggtgc gtgcgatcgg cctctcggaa gtctcggccg
     1501 cgacgctgcg ccgcgcccac gccgtgcatc cggtcagcgc cctgcagagc gaatattcgc
     1561 tctggtcgcg caaccccgaa atcgccggcc tcgaagcctg ccgggaggtc ggtgcgagct
     1621 tcgtcgcctt cagcccgatc gcgcgcggct ttctcagcgg ccggttgcgc gaggtggcga
     1681 cgctcgacgc ccaggacatt cgccgcaaca tgccgcgctt ccagccggcc gcgttcgacg
     1741 cgaacctctc gttgttggag agttttaccg cgctcgcgga cgaagccggc tgcacgccgg
     1801 cgcagctggc gctggcctgg ttgctcgcgc aggatgcgag cctcgtgccg attcccggca
     1861 cgacccgcgt cgatcacctg gccgagaacg tcggcgccgc atcggtcgcg ctcgaggccg
     1921 gcctgctcgc gcgggtcgac gccacgatca accggcacac cgtgaagggc ccacgctaca
     1981 acgcgacgac gcaggccgag atcgacaccg aggaatttcc ggcgtgagac gccgcagcat
     2041 gctgaccgct gtcggcggcg cggcgctgct gcgcgtcgcc gaagcggagg cgacgtggcc
     2101 ggcgcggccg gtgacgctgg tcgtgccgtt cgccgccggc ggcatcgccg atctcaccgc
     2161 gcgcaccgtc acgaaggcga tggcaaccct cctcggccag gcgatcgtcg tcgacaaccg
     2221 gccgagcgcc ggcagcgtcg tcggctcggg catggtggcc catgccacgc ccgacggcta
     2281 caccttgctg ctgatgagca acgccaacgc catca
//
LOCUS       DASRWS010000230         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3540665, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000230 DASRWS010000000
VERSION     DASRWS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540665"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFF72_04260"
     CDS             complement(<1..585)
                     /locus_tag="VFF72_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV92404.1"
                     /translation="MTTTPTFHEARDLLLRHRTDYDSAYREFRWPVLERFNWALDHFD
                     AIARGNESTALHIVGEDGSETRRSFAEMSVRSSQVANHLRGLGVKRGDRILLMLGNEL
                     ALWEVMLASIKLGAVVIPATSLLTTDDLRDRLERGAVRHIVTSSALAAKFADLPGRFT
                     RVAIGAPVAGWQDYADSEKAATAFVADGETRADDP"
     gene            complement(737..>2345)
                     /locus_tag="VFF72_04265"
     CDS             complement(737..>2345)
                     /locus_tag="VFF72_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92405.1"
                     /translation="IGRHRGKVAAGMLTLVAIVAGLVGTISQARRAERQSAIASQQAE
                     IARHERDGAVEQARLQRGSNEFLQLLLRDAAGGDPGTIRRQLDRAVALLETTRFEAPI
                     VKVALLRQTAGRYVEIGDVATGTVLLRRAIASIEGTPLADRTSAVPVNLACSLARDLH
                     EMDDQPGALAELDRADHLIAAGADVDLPSRVECHVTRAFAESALGRFERGVALDRDAL
                     RQLAGAGIRQGEQQRVTRSALTYTLAAAGHAAEAMPLARALLAETVAGTGRDSIGALR
                     RSAIVTSLTRIGGDPLAALGLADADLVDGARLLGSQTDAALELERGLDLLALGRDAQA
                     AKPLGRAGEAAVATGRRVLVLPAGLAETDALIGAGRIDTASERWNQLAPLRDIAARQG
                     RPEQITALRIAAMLSRARGDTAQAIGELDVAQKLVDAAGGVAHPLAFAVASARADLAI
                     AAAEPDTVALPFAERALAAARRTSIDERRSSQVGQALLLRARIEAAGGRADDARRDAA
                     AAAAQLTPTLGADAAATQQAMALASNR"
BASE COUNT          290 a          833 c          888 g          334 t
ORIGIN      
        1 tggatcgtcg gcgcgggtct cgccgtcggc gacgaaagcg gtcgctgcct tctcgctgtc
       61 tgcgtagtcc tgccagcccg cgaccggcgc gccgatcgcc acgcgcgtga agcgaccggg
      121 caggtcggcg aacttggcgg ccagggcact gctcgtcacg atgtgcctca ccgcgcctcg
      181 ctcgagccgg tcgcgcaggt cgtcggtcgt cagcagcgat gtcgcgggaa tgacgaccgc
      241 gccgagcttg atcgacgcca gcatcacctc ccacagcgcc agctcgttgc cgagcatcag
      301 caggatccgg tcgccgcgct tcacgccgag gccgcgcagg tgattggcga cctgcgacga
      361 gcgcaccgac atttccgcga agctgcgcct ggtctcgctg ccgtcctcgc cgacgatgtg
      421 cagcgccgtc gattcgttgc cgcgggcgat cgcgtcgaag tgatcgagcg cccagttgaa
      481 acgctcgagc accggccagc gaaactcgcg gtaggcgctg tcgtagtcgg tgcggtggcg
      541 cagcagcagg tcgcgcgcct cgtggaaggt tggtgtggtc gtcatggcgg actcgggctt
      601 ggacgaaggg ccgccgctcg gcgctgcgcc gcagcggcag gggactgcgt gcaagaccga
      661 tattgccaga gccggagagc gactcgtcgg ggcgtggcga gcgccccccg gagtgcctcc
      721 cgcggcgacc gctggctcag cgattcgatg ccagcgccat cgcctgttgc gtggccgcgg
      781 catcggcgcc cagcgtcggc gtcagctggg cggcggccgc cgccgcatcg cgccgggcat
      841 cgtcggcgcg gccgccagcc gcttcgatgc gggcgcgcag gagcagtgcc tgcccgacct
      901 ggctcgacct ccgctcgtcg atcgacgtcc ggcgagcggc cgccagggcg cgttcggcga
      961 aaggcagcgc gaccgtgtcg ggctcggcag cagcgatggc cagatcggcg cgcgccgacg
     1021 cgaccgcgaa cgccagcgga tgcgcgacgc cgccggccgc atcgacgagc ttttgcgcca
     1081 cgtcgagctc gccgatcgcc tgtgcggtgt cgccgcgggc gcggctcagc atcgccgcga
     1141 ttcgcagcgc ggtgatctgc tcgggtcgcc cttgcctggc ggcgatgtcg cgcaagggcg
     1201 cgagctggtt ccagcgctcg ctcgcggtgt cgatccggcc ggcgccgatc agcgcgtctg
     1261 tttcggccag gccggccggc aggacaagca cccggcgtcc cgtggccacg gcggcctcgc
     1321 cggctcggcc aagcggcttc gcggcttgcg catcgcggcc cagcgccagc agatcgagcc
     1381 cgcgttcgag ctcgagtgcc gcgtcggttt gcgagccgag caggcgtgcg ccatcgacga
     1441 ggtcggcgtc ggccaggccg agcgcggcga gtggatcgcc gccgatgcga gtcaggctcg
     1501 tcacgatggc cgagcggcgc agcgcgccga tcgaatcgcg acccgtgccg gccaccgttt
     1561 ccgcgagcag ggcccgcgcc agcggcatcg cctcggcggc gtgaccggcg gcggccagcg
     1621 tgtaggtgag ggcgcttcgc gtcacccgct gctgttcgcc ctgacggatg ccggcgccgg
     1681 ccagttgccg caaggcgtcc cggtcgaggg cgacgccgcg ctcgaagcgg ccgagcgcgc
     1741 tctcggcgaa ggcccgcgtg acgtgacact cgacccggct gggcagatcc acgtcggccc
     1801 cggcggcgat caggtggtcg gctcggtcga gctcggccag cgccccgggt tggtcgtcca
     1861 tctcgtgcag gtcgcgcgcc agcgagcagg cgaggttgac gggcacggcg ctggtccggt
     1921 cggcgagcgg tgtcccttcg atcgaggcga tggcccggcg cagcagcacc gtgccggtgg
     1981 cgacgtcgcc gatctcgaca tagcggcctg cggtctggcg caacagcgcg accttgacga
     2041 tgggcgcctc gaagcgggtc gtctcgagca gggcgaccgc gcggtcgagc tggcggcgga
     2101 tcgtgcccgg atcgccgccc gccgcatcgc gcaagaggag ctgcaggaac tcgttgctgc
     2161 cgcgttgcag gcgggcctgc tcgacggctc cgtcgcgctc gtggcgggcg atctctgcct
     2221 gctgcgatgc gatggcgctt tgccgctcgg cgcggcgcgc ctggctgatg gtgccgacca
     2281 gcccggccac gatcgcgacg agcgtcagca tgccggcggc gaccttgccg cgatggcggc
     2341 cgatg
//
LOCUS       DASRWS010000231         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_956749, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000231 DASRWS010000000
VERSION     DASRWS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956749"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VFF72_04270"
     CDS             complement(<1..1092)
                     /locus_tag="VFF72_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HZV92406.1"
                     /translation="MNDLMQQLQARLGPNAKAIRALMAPLVVVMILAMMVLPLPPFAL
                     DVLFTFNIAMALMVMMVAAYMVRPLDFAAFPAVILLTTLLRLSLNVASTRVVLLQGHT
                     GAAAAGHVIESFGHFLIGGNFAVGLIVFAILVVINFVVVTKGAERIAEVGARFTLDAM
                     PGKQMAIDADMNAGLIDQNEAKRRRAEVGDEAEFFGSMDGASKYVRGDAMAGLLILFI
                     NIIGGFIIGVFQHDLSASQAADSYILLAVGDALVAQIPALLISVAAAMVVSRVGKDQD
                     VGSQITKQVFSSPRSLAVVAGVLAVLGSIPGMPHTVFLLLAAGLGYGAWTLRERQQRA
                     RNAPKPAPPPPEANAEATWDDLQPVDTLGL"
     gene            complement(1138..2274)
                     /locus_tag="VFF72_04275"
     CDS             complement(1138..2274)
                     /locus_tag="VFF72_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZV92407.1"
                     /translation="MADTSAQDKNLPASERRLQKAREEGQVARSRDLGHFAAIAAGAG
                     LVMVAAPHVSAMLREAVATSLRFDRAQAVGTDTMARRLGDLTATWAWTALPFGVAMIA
                     VAVAGGLAISGWTWTFKPMTPKFTFLNPLAGIGRVFSKQQLVDTIKACFLALVLGVIG
                     ASWLKGHAMDFAGVLAMPLPAAISASGRAMTGGLVLLLVALALFAAVDVPLQLRMHAT
                     RLKMSRQELKQEHKELEGNAEIKGKVRARMREMTKRRMMAAVPKADLVVMNPTHYAVA
                     LRYDDKTMAAPRVVAKGADLLALRIRDLARDSKVPVLEAPVLARALYAHAELDREIPA
                     RLFSAVAQVLAYVYQLRTAMAGTGAMPGSLPVLDVPAELDPHAA"
BASE COUNT          371 a          821 c          869 g          332 t
ORIGIN      
        1 gaggccgagc gtgtcgactg gctgcaggtc gtcccaggtc gcctcggcgt tcgcctcggg
       61 cggcggcggc gccggcttcg gcgcgttcct ggcgcgctgc tggcgctcgc gcagcgtcca
      121 agcgccgtag ccgaggccgg cggccaggag caggaagacg gtgtgcggca tgccgggaat
      181 cgagcccagc accgcgagca cgccggcgac gacggcgagc gaacgcggcg aggagaagac
      241 ctgcttcgtg atctggctgc cgacgtcctg gtccttgccg acgcgcgaga cgaccatcgc
      301 cgcggcgacc gagatcagca gcgccgggat ctgcgccacc agcgcatcgc cgacggcgag
      361 caggatgtag ctgtcggcgg cctgcgaggc gctcaggtcg tgctggaaca cgccgatgat
      421 gaagccgccg atgatgttga tgaacaggat cagcaggccc gccatcgcgt cgccgcgcac
      481 gtacttggag gcgccgtcca tcgagccgaa gaactcggcc tcgtcgccga cctcggcgcg
      541 ccggcgcttg gcctcgttct ggtcgatcag gccggcgttc atgtcggcgt cgatcgccat
      601 ctgcttgccg ggcatcgcgt cgagggtgaa gcgggcgccg acctcggcga tccgctcggc
      661 gcccttggtg acgacgacga agttgatgac gacgaggatg gcgaagacga tcaggccgac
      721 cgcgaagttg ccgccgatca ggaagtggcc gaacgactcg atcacgtgcc cggccgccgc
      781 ggcgccggtg tgtccctgca gcagcacgac ccgcgtcgag gcgacgttga gcgacagccg
      841 caacagcgtc gtcagcagga tcaccgccgg gaaggcggcg aagtcgagcg gccgcaccat
      901 gtaggcggcg accatcatca ccatcagggc catggcgatg ttgaaggtga acagcacgtc
      961 gagcgcgaac ggcggcagcg gcagcaccat catcgccagg atcatcacga ccacgagcgg
     1021 cgccatcagg gcgcggatcg ccttggcgtt cgggccgagc cgggcctgca gctgttgcat
     1081 caggtcgttc atggggcgtc gtcgtccttc ggggagttcg ctcggggcct gctggcgcta
     1141 ggccgcgtgc gggtcgagct cggccggcac gtcgagcacc ggcaggctgc ccggcatcgc
     1201 gccggtgccg gccatcgccg tgcgaagctg gtacacgtag gccagcacct gcgccacggc
     1261 cgagaagagc cgcgccggaa tctcgcgatc gagctcggcg tgcgcgtaga gcgcccgcgc
     1321 cagcaccggc gcctcgagca ccggcacctt gctgtcccgg gcgagatcgc ggatccgcag
     1381 ggccagcagg tcggcgccct tggcgacgac gcgcggcgcg gccatcgtct tgtcgtcgta
     1441 ccggagcgcc accgcgtagt gggtcgggtt catgacgacc agatcggcct tcggcacggc
     1501 ggccatcatc cggcgcttgg tcatctcgcg catccgcgcc cgcaccttgc ccttgatctc
     1561 ggcgttgccc tcgagctcct tgtgctcctg cttgagctcc tggcggctca tcttgaggcg
     1621 cgtcgcgtgc atgcgaagct gcagcggcac gtcgaccgcg gcgaacaggg ccagggcgac
     1681 gaggagcagg acgaggccgc cagtcatcgc ccggcccgaa gcggagatcg ccgccggcag
     1741 cggcatcgcc agcacgccgg cgaagtccat cgcgtgcccc ttcagccagg acgcgccgat
     1801 gacgccgaga acgagcgcga ggaagcacgc cttgatcgtg tcgacgagct gctgtttcga
     1861 gaagacgcgg ccgatgccgg cgagcggatt gaggaacgtg aacttcggcg tcatcggctt
     1921 gaacgtccac gtccagccgc tgatcgccag tccgccggcg acggcgacgg cgatcatcgc
     1981 cacgccgaag ggcagcgccg tccaggccca ggtcgccgtc aggtcgccga ggcgccgggc
     2041 catcgtgtcg gtgccgaccg cctgcgcgcg atcgaagcgc agcgacgtgg cgacggcctc
     2101 gcgcagcatc gcgctgacgt ggggcgccgc caccatgacg aggccggcgc cggccgcgat
     2161 cgccgcgaaa tgaccgaggt cgcgcgatcg cgcgacctgc ccttcctcgc gcgccttctg
     2221 gagtcggcgc tcggaggcgg gaaggttctt gtcctgtgcg gaggtgtcgg ccatgggcga
     2281 aagatcagct ggcgacgatt gttccggccg acccgggaaa cttgagccgg ataagcgacc
     2341 ggaaggccgc gctattcgcc gcctcgccgc ggcggtcacg gccggccgaa gcc
//
LOCUS       DASRWS010000232         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2111173, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000232 DASRWS010000000
VERSION     DASRWS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111173"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2435)
                     /gene="lon"
                     /locus_tag="VFF72_04280"
     CDS             complement(<1..2435)
                     /gene="lon"
                     /locus_tag="VFF72_04280"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZV92408.1"
                     /translation="MPNDTSLPAGVTTLPLLPLRDVVVFPHMVIPLFVGRPKSIKALD
                     AAMDAGKHILLVAQKQAAKDDPTAADLYDIGSIATVLQMLKLPDGTVKVLVEGTQRAH
                     IRTVEEQGEYLSAQAVALPGDPGAAHEVEAMRRALLTQFDAYVKLNKKIPPEILTSMS
                     GIDDGGRLADAIAAHLPLKLEQKQQILEMSDVGKRLEHLLGVLEGEVDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGEGDENADLEEMEKKIRGAKMSREAEGKAMAE
                     LKKLRMMSPMSAEATVVRNYIDTLLGLPWRKKSKISKDLAHAVKVLDADHYGLEKVKE
                     RIVEYLAVQQRVDRLKAPILCLVGPPGVGKTSLGQSVAKATGRKFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMTKVAVRNPLFLLDEVDKMGQDFRGDPSSALLEVLDPEQ
                     NNTFVDHYVEVEYDLSDVMFVATANTMNIPAPLLDRMEVIRLSGYTEDEKIAIAKQYL
                     LPKQLKHNGLAPEEIHVAESALRDIVRYYTRESGVRNLERDISKICRKVVKSLLLRES
                     EGKARKARGPVDVNSKNLDKYLGVRRFTYGIAEKKNQVGQVTGLAWTEVGGDLLTIEA
                     VALPGKGKTTSTGKLGDVMNESIAAALSVVRSRAKQLGIAPDFYQKTDIHIHLPEGAT
                     PKDGPSAGAAVTTAMVSMLTGIPVRCDVAMTGEITLRGEVLPIGGLKEKLLAAGRGGV
                     KTVLIPEENVKDLAEVPDEIKNRIDIQPVRWIEQVLEHALESHPVPAVDDKDKPEAPV
                     PPPPASDEQATLI"
     gene            complement(2776..>3923)
                     /gene="clpX"
                     /locus_tag="VFF72_04285"
     CDS             complement(2776..>3923)
                     /gene="clpX"
                     /locus_tag="VFF72_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZV92409.1"
                     /translation="CNDIIREEGTGSETARADRSKLPTPHEIRQILDQYVIGQEPAKK
                     ILSVAVYNHYKRLESGGKDDEVELAKSNILLIGPTGSGKTLLAQTLARLLDVPFVIAD
                     ATTLTEAGYVGEDVENIIQKLLQKCDYDIEKAQRGIVYIDEIDKISRKSDNPSITRDV
                     SGEGVQQALLKLIEGTIASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLEKIIRQ
                     RTAKTGIGFGAEVASPDDRKTMTILLRDTEPEDLIKFGLIPEFVGRLPVVATLDELDE
                     AALIQILVEPKNALIKQYQKMFRMEAVELEVREAALRAIARKALARKTGARGLRSILE
                     QVLLDVMYDLPSLSNLARVVVDESTIANDGKPLLMFADQPKVAASAK"
BASE COUNT          544 a         1292 c         1303 g          784 t
ORIGIN      
        1 ttgatcagcg tcgcctgctc gtcgctcgcg ggtggcggcg gcaccggcgc ttccggcttg
       61 tccttgtcat cgacagcagg caccgggtgc gattcgagcg cgtgctcgag cacctgctcg
      121 atccagcgca ccggctggat gtcgatgcgg ttcttgatct cgtcgggcac ttcagcgagg
      181 tccttgacgt tctcctcggg gatcagcacg gtcttcacgc cgccgcggcc cgcggcgagc
      241 agcttctcct tcaggccgcc gatcggcagc acttcgccgc gcagcgtgat ctcgcccgtc
      301 atcgccacgt cgcagcgcac cgggatgccg gtcagcatcg acaccatggc cgtcgtcacc
      361 gcggcgcctg cggacgggcc gtccttgggt gtcgcacctt ccggcaagtg aatgtggata
      421 tcggtcttct ggtagaagtc cggcgcgatg ccgagctgct tcgcgcgcga gcgcacgacc
      481 gacaacgcgg cggcgatcga ttcgttcatc acgtcgccga gcttgccggt ggacgtggtc
      541 ttgcccttgc cgggcagcgc cacggcctcg atcgtcagca ggtcgccacc gacttcggtc
      601 cacgccaggc cggtgacctg gccgacctgg ttcttcttct cggcgatgcc gtacgtgaag
      661 cggcgcacgc cgaggtactt gtcgaggttc ttcgagttca catcgaccgg gccgcgtgcc
      721 ttccgcgcct tgccctcgct ctcgcgcagc agcagcgact tcaccacctt gcggcagatc
      781 ttgctgatgt cgcgctcgag attgcgcacg cccgattcgc gcgtgtagta gcgcacgatg
      841 tcgcgcagcg cgctctcggc gacatggatc tcctcgggcg ccaggccgtt gtgcttgagc
      901 tgcttgggca gcaggtactg cttggcgatc gcgatcttct cgtcctcggt gtagcccgag
      961 aggcgaatga cttccatccg gtcgagcagc ggcgccggga tgttcatcgt gttggcggtg
     1021 gcgacgaaca tcacgtcgga caggtcgtac tcgacctcga cgtaatggtc gacgaatgtg
     1081 ttgttctgct cgggatcgag cacctcgagc agcgccgacg acggatcgcc gcggaagtcc
     1141 tgccccatct tgtcgacttc gtcgagcagg aacagcggat tgcgcacggc aaccttcgtc
     1201 atgttctgca ggatcttgcc cggcatcgag ccgatgtacg tgcggcgatg gccgcggatc
     1261 tcggcctcgt cgcgcacgcc gccaaggctc atgcgcacga acttgcgtcc ggtggccttc
     1321 gccaccgact ggccgagcga cgtcttgccg acgccgggcg gcccgacgag gcacaggatc
     1381 ggcgccttca gccggtcgac gcgctgctgg accgccaggt attcgacgat gcgctccttg
     1441 accttctcga ggccgtagtg atcggcgtcg agcaccttga ccgcgtgcgc gaggtccttg
     1501 ctgatcttgc tcttcttgcg ccacggcagg ccgagcagcg tgtcgatgta gttgcgcacg
     1561 acggtcgctt cggcggacat cggcgacatc atgcgcagct tcttcaattc ggccatcgcc
     1621 ttgccttcgg cctcgcgcga catcttcgcg ccgcggatct tcttttccat ctcctcgagg
     1681 tcggcgttct cgtcgccctc gccgagttcc ttctggatgg ccttgacctg ctcgttcagg
     1741 tagtactcgc gctggctctt ctccatctgc cgcttgacgc ggccgcggat gcgcttctcg
     1801 acctgcagga tgtcgacctc gccttcgagc acgccgagca ggtgctcgag gcgcttgccg
     1861 acgtcgctca tctcgaggat ctgctgcttc tgctcgagct tgagcggaag gtgcgcagcg
     1921 atcgcgtcgg cgaggcggcc gccgtcgtcg atgcccgaca tcgacgtgag gatttccggc
     1981 gggatcttct tgttgagctt gacgtacgcg tcgaactgcg tcagcagcgc gcgacgcatc
     2041 gcctcgactt cgtgcgcggc gcccgggtca ccgggcaacg ccaccgcctg cgccgacagg
     2101 tattcgcctt gctcctcgac ggtgcgaatg tgcgcgcgct gcgtgccttc gacgagcacc
     2161 ttcacggtgc cgtcgggaag cttcagcatc tgcaggaccg tcgcgatgct gccgatgtcg
     2221 tagagatcgg cggccgtcgg gtcgtccttg gccgcctgct tctgcgcgac caggagaatg
     2281 tgcttgccgg catccatcgc ggcgtcgagc gccttgatgg acttcgggcg tcccacgaac
     2341 agcgggatca ccatgtgcgg gaacacgacg acgtcgcgca gcgggaggag cgggagcgtc
     2401 gtgacgccgg ccgggaggga tgtgtcgttg ggcattcgtg cctcgggtgg agaattcatc
     2461 ggtctacatg gggccgtggg gggtcccttc aaggagaagg gtacccggtg gccgggctcc
     2521 gccgtgcggc gaattgacag tcttacgtct ggcacgccgg cgccatccac caaggggcgt
     2581 ggtcagcggg accaccggcg atttccgccc gggatcgggt ccaccgtcct cacgctaaca
     2641 cgctgatccg gcggatcgtt ccgggagcgc gcgttgaacg tcgacgaagt cgccggtcga
     2701 acgagcgccg ttattgcgtc acgccggcat tcgccggcgc gcgggtgcgg gtgcccgtcg
     2761 gcgtgacgga acgacctact tggccgatgc cgctaccttc ggctggtcgg cgaacatgag
     2821 cagcggcttg ccgtcgttgg cgatcgtcga ttcgtcgacg acgacacgcg cgagattcga
     2881 cagcgacggc aggtcgtaca tcacgtcgag cagcacctgc tcgaggatgg accgcaggcc
     2941 gcgcgcgccg gtcttgcgcg ccagtgcctt gcgggcgatc gcccgcagcg cggcttcgcg
     3001 cacctccagc tcgaccgctt ccatgcggaa catcttctgg tactgcttga tgagcgcgtt
     3061 cttcggctcg accaggatct ggatcagcgc ggcctcgtcg agctcgtcga gggtcgcgac
     3121 caccggcagg cgacccacga actcgggaat gaggccgaac ttgatcaggt cctcgggctc
     3181 ggtgtcgcgc agcaggatcg tcatggtctt gcggtcgtcg ggcgacgcca cctcggcgcc
     3241 gaagccgatg cccgtcttgg ccgtgcgctg gcggatgatc ttttccagcc cgtcgaaggc
     3301 gccgccgcac acgaacagga tgttggtcgt gtcgacctga acgaactcct ggttcgggtg
     3361 cttgcggccg ccctgcggcg gcacgctggc gatggtgccc tcgatcagct tcaggagcgc
     3421 ctgctgcacg ccctcgcccg acacgtcacg ggtgatcgac gggttgtcgc tcttgcgcga
     3481 gatcttgtcg atctcgtcga tgtagacgat gccgcgctgc gccttctcga tgtcgtagtc
     3541 gcacttctgc agcagcttct ggatgatgtt ctcgacgtcc tcgccgacgt agccggcttc
     3601 ggtgagcgtc gtggcatcgg cgatgacgaa cggcacgtcg agcagccgcg cgagtgtctg
     3661 cgcgagcagc gtcttgcccg atccggtggg cccgatcagc aggatgttcg acttggcgag
     3721 ctcgacctcg tcgtccttgc cgcccgattc gagccgcttg tagtggttgt agacggcgac
     3781 cgagaggatc ttcttcgccg gctcctgccc gatcacgtac tggtcgagga tctgccggat
     3841 ttcgtgcggc gtgggcagct tgctgcgatc ggcgcgcgcg gtttcgctgc cggtgccctc
     3901 ctcgcggatg atgtcattgc aaa
//
LOCUS       DASRWS010000233         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_388433, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000233 DASRWS010000000
VERSION     DASRWS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388433"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFF72_04290"
     CDS             <1..770
                     /locus_tag="VFF72_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZV92410.1"
                     /translation="VATEGGALPESRLQPFIDDVDAARHARPFDRADLDSTSLGVAVD
                     ALLLPGNSQRPWRALVNLQPAVGHALDIARIRSAIAGVPGAQVVAVKPELDAIYARYL
                     HQATWQAGLGGLAVVLLLALYLRRLRRLVHVLQPLAAATLVVLAALTASHASLGILHL
                     VGLLLTVAIGSNYALFFDHLAQRGSADADADTLASLLLANLTTVVSFGLLATSRIPVL
                     QAVGEVVAPGALLCLVFSAAFIARPGLAQRRAKIGGP"
     gene            767..1696
                     /locus_tag="VFF72_04295"
     CDS             767..1696
                     /locus_tag="VFF72_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZV92411.1"
                     /translation="MNRNPIRGPIAAPATGCRSGIRPASSTWPTYLKASIACHAGAAV
                     TAAALPQAWPWALGAVVANHAVLTGAGLWPRSNWLGRNWTRLPAASVGRREIAVTIDD
                     GPEPDITPKVLDLLDAHGVRATFFAIGHQAAREPALCRQIVRRGHSVENHSDRHSPAF
                     SLLGPRAMAREVAAAQRRLADITGVAPRYFRAPAGLRNPFLSSVLDRLGLELVSWTRR
                     GFDTVRREPGRIVAALSRDLAAGDILLLHDGHAARTPEGRAVVLDVLPALLTRAAAAG
                     LRPVTLTAAFAGAELDCDAAGRIASDAASAGRR"
     gene            1693..2517
                     /locus_tag="VFF72_04300"
     CDS             1693..2517
                     /locus_tag="VFF72_04300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV92412.1"
                     /translation="MSATLAAVAWRSLVESAALPYAGGGRFALHFARGKLRWDPVFRH
                     LVGSGLIAPRARVLDIGCGQGLLASLLGAAGRLARRGGWPVDWAAAPSDVRVVGIELA
                     PLEVARARDALGADADFVCGDMRCTAFPNVDAVVILDALHYVSHAEQVEILRRVRDAL
                     PGGGCLMLRVGDASSRLAFAIGQGVDRLVALARGGRARPRAGRSLAAWQALLSGLGFD
                     VASLPMHRGTPFVANVLLVARVRVTPTVPIAPVTAIAPVTPVTPVTSITSITPVAP"
BASE COUNT          295 a          993 c          863 g          401 t
ORIGIN      
        1 cggtcgcgac cgagggcggc gcattgcccg aatcgcgcct gcagcctttc atcgacgacg
       61 tcgatgccgc acgccatgcc aggccgttcg accgcgccga tctcgactcg acgtcgctcg
      121 gcgtcgcagt cgacgcactc cttcttccgg gcaactcgca acgcccgtgg cgcgcgctcg
      181 tcaacctcca gccagcggtc gggcatgcgc tcgacatcgc ccgcatccgc tcggccatcg
      241 ccggcgtgcc tggcgcgcag gtggttgcgg tcaagcccga gctcgatgcc atctatgcgc
      301 gctatctgca ccaggcgaca tggcaggccg gcctcggcgg cctcgctgtc gtgctgctgc
      361 tggctctcta cctgcgccgg ctgcgccgcc tcgtgcacgt gctgcaaccg ctcgcggcgg
      421 cgaccctggt cgtgctggcg gcgctgacgg cgagccatgc ctcgctcggc atcctgcacc
      481 tcgtcggcct gctgctgacc gtggcgatcg gctccaacta cgccctcttc ttcgaccacc
      541 tcgcccagcg cggcagcgcc gacgccgacg ccgacacgct ggcttcgctg ctgctcgcca
      601 acctcaccac cgtcgtctcg ttcggcctgc ttgccacgtc acgcattccg gtgctgcagg
      661 cggtcggtga agtcgtcgcg ccgggcgccc tgctttgcct cgtcttctcc gccgcattca
      721 tcgcccggcc cggcctggcg cagaggcgtg cgaagatcgg cgggccatga atcgcaatcc
      781 gatccgcggt cccatcgctg cgccagccac gggctgtcga agcgggatcc ggcctgcctc
      841 ttcgacatgg cccacctatc tcaaggcaag catcgcctgc cacgccggtg ccgcggtgac
      901 cgccgcggcg ttgccccagg cgtggccctg ggcgctcggc gccgtcgtcg ccaaccacgc
      961 ggtgctgacc ggcgccgggc tgtggccccg gtcgaactgg ctcgggcgca actggacccg
     1021 cctcccggcg gcgtcggtcg gtcgacgcga aatcgcggtg acgatcgacg atggacccga
     1081 acccgacatc acgccaaagg tcctcgatct cctcgacgcc cacggcgtgc gggcgacgtt
     1141 cttcgcgatc ggacaccagg ccgcgcgcga gccggccctg tgccggcaga tcgtccggcg
     1201 cgggcacagc gtcgagaacc acagcgatcg ccactcgccc gccttctcgc tgctcgggcc
     1261 gcgcgcaatg gcacgcgagg tcgcggccgc acagcgccga ctggccgaca tcaccggcgt
     1321 cgcgcctcgc tacttccgcg ccccggccgg tctgcgcaat ccgttcctct cttcggtgct
     1381 cgaccggctc ggcctcgagc tcgtgagctg gacgcggcgc ggcttcgaca cggtccgccg
     1441 cgaacccgga cgcatcgtcg ccgcgctctc gcgcgatctc gccgcgggcg acatcctgct
     1501 gcttcacgac ggccacgcgg cacgaacgcc tgaaggccgt gccgtcgttc tcgacgtgct
     1561 gcctgccctg ctgacccgcg ccgcggccgc cggcctgcga cccgtcacgc tgaccgcggc
     1621 gttcgcaggc gcggagctcg actgcgacgc cgccggccgc atcgccagcg acgcggccag
     1681 cgcgggcagg cggtgagcgc gacgctcgct gccgtcgcct ggcgctcgct cgtcgagagc
     1741 gccgcgttgc cgtacgccgg cggcggccgg ttcgccctcc atttcgcgcg cggcaagctg
     1801 cgctgggatc ccgtctttcg ccacctcgtc ggcagcggtc tgatcgcgcc gcgggcacgg
     1861 gtgctcgaca tcggctgcgg ccaggggctg cttgccagcc tgctcggtgc cgccggtcgg
     1921 ctggcccgcc gtggcggctg gccggtcgac tgggcggcag cgccgagcga tgtccgcgtc
     1981 gtcggcatcg agctggcgcc gctcgaggtg gcccgggcac gcgacgcgct cggcgccgac
     2041 gccgacttcg tctgcggcga catgcggtgc acggcttttc cgaacgtcga tgcggtcgtc
     2101 atcctcgacg ccctgcatta cgtgagccac gccgagcagg tcgagatcct gcgccgcgtg
     2161 cgcgatgcac tgcccggcgg aggatgcctc atgctgcgcg tcggcgacgc gtcgtcgagg
     2221 ctcgcattcg cgatcggcca gggcgtcgac cggttggtgg ccctggcgcg aggcggtcgg
     2281 gcgaggccgc gtgcggggcg cagcctcgcg gcctggcagg cactgctctc cggtctcggc
     2341 ttcgatgtcg cgagcctgcc gatgcatcgc ggcacccctt ttgtcgccaa cgtcctgctc
     2401 gtggcaaggg tgcgcgtcac gccgacggtg ccgatcgcgc cggtcacggc gatcgcgccc
     2461 gtcacgccgg tcacgccggt cacgtcgatc acgtcgatca cgccggtcgc gccatgacga
     2521 cgccatgacg acgccatcga cgctcgacca cg
//
LOCUS       DASRWS010000234         5812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1529617, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000234 DASRWS010000000
VERSION     DASRWS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529617"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFF72_04305"
     CDS             <1..496
                     /locus_tag="VFF72_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859634.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV92413.1"
                     /translation="YVELIRNTPFLVQLFFFFFALPAIGLRWSAHTAALTAMVVNLGA
                     YATEIIRAGIEAIPKGQIEAGLALNLKAHEIFRFVVLKPALKAIYPALTSQFILLMLS
                     SSVVSVISADDLTSVASNLQSQTFRSFEIYIVVAGIYLALALSFSTLFRVVYRHAFNY
                     PDRR"
     gene            586..1239
                     /locus_tag="VFF72_04310"
     CDS             586..1239
                     /locus_tag="VFF72_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV92414.1"
                     /translation="MRTFGPGDFLYILAAARWTLGLSLIAFVGGAVGGLAVALSRTSD
                     IGWLRNASAAFIQLFQGTPLLLQLFLIFFGAPVLGFDINPWVAAGIALVLNSSAFLGQ
                     IWRGCIEAIPRGQREAADSLSLSYRDRMRDVILPQALRIAIAPTVGYMVQIIKGTSLA
                     AIIGFTEITRAGQIINNATFQPLIVFSAVAAIYFVLCWPLSLLAAGIERRHRRALAR"
     gene            1300..2103
                     /locus_tag="VFF72_04315"
     CDS             1300..2103
                     /locus_tag="VFF72_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZV92415.1"
                     /translation="MIRSPQRRRVAISLANVGLAAALPAWLVTARAQTVDEIKKKGEL
                     TVGLLVDFPPYGTVNSANEPDGYDADVARLLAKDWGVKLKLVPVTGPNRIPFLLTNKV
                     DLLIASLAITPERAKQVLFSKPYAAATIVLLGSKKAHIKGPADLKGLRIGVARASTQD
                     IALTAIAPPGTEIRRFDDDASGMQALMSGQVDALGCSTTVAAQIEKRAPANTYDEKFV
                     LRQQVMGVAMRPGQTELEKAVNAFIDRNKANGELDKLYEKWLGTKLPAM"
     gene            2129..2959
                     /locus_tag="VFF72_04320"
     CDS             2129..2959
                     /locus_tag="VFF72_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZV92416.1"
                     /translation="MSDPGAEPIILIEGLDKWFGHFQVLTDIDLSVRAGERIVICGPS
                     GSGKSTLIRCINRLETAQKGRIVVDGVDLAAGRRNVDRVRREVGMVFQQFNLFPHLTA
                     LQNCTLAPMRSRGMSKDEAEATAMKYLTRVRIPEQANKYPSQLSGGQQQRVAIARALC
                     MNPKIMLFDEPTSALDPEMVKEVLDTMIGLAEDGMTMLCVTHEMGFARSVADRVVFMA
                     EGRIVEEAPPEQFFAHPQHEKTRNFLGQILVSHHTRPERLRTEPASIPPVAPVFGPAP
                     "
     gene            3082..4287
                     /locus_tag="VFF72_04325"
     CDS             3082..4287
                     /locus_tag="VFF72_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92417.1"
                     /translation="MTDVVFSNKGALSKPSKASVMLPQFQFPAFELTFSDKTVEEMLK
                     KDSKDWARARTKMGEETAREYKVYKEAVEKGLAELDAMCKGKDKATCDKSVATFEAVL
                     KRATESVDASIRKIPPQVWSDWIKTRDKYKKYQYKTAVSIGKSVLGVISSAASLAGAV
                     GTYGATLPLSIIALTRTSIGLATDVKNAALEVEHVEKVIRTDAKILEAAFKKGGKAAL
                     VAGSMGVKAVQSILNTDIGPSMKTLTSNVTLWGNKFAGLEVTVTRLGGSVTEFVDKGE
                     ELTKFIKTLPVGKERKKMEEKQAKLEKATAKLLSANDAMNDRMEKAQKKFNTNCVPAI
                     KTLNAAYPKLATKFEDLAPVVVSLGLGLGGADYSAMASGSVEGLKAMLEVGNELAGAT
                     KEVYSALKK"
     gene            4472..4633
                     /locus_tag="VFF72_04330"
     CDS             4472..4633
                     /locus_tag="VFF72_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92418.1"
                     /translation="MPKRTLIALFILVDILVICGLGYAFRNYFWHYCETNQCKSNPKG
                     VYSASKPSI"
     gene            4973..>5812
                     /locus_tag="VFF72_04335"
     CDS             4973..>5812
                     /locus_tag="VFF72_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZV92419.1"
                     /translation="MAAVAEIAIDMATPLELDLASIYPIPLLLAAYTRRRQLLWSLAL
                     LLGFATLVVYALHAEALSPMLKGEVLSNRMFDVVALLVATGVLHHWMRSLEVRESQSR
                     LLGEQNRRLETANRLLREHEAQILKQNEELDRRHKDVVASSARMSQMLVAVSHDIRTP
                     IQTIGLVAELMRRTGEDRALADRVPQMAQRLQSNAATLVTMVSDLLDLARFDAGRTEL
                     RQTTFTLDELVASKCRDLGALAETKSLALIARTPSVPLWVRSDRAKLERVLSNLVGNA
                     IKFT"
BASE COUNT         1067 a         1972 c         1846 g          927 t
ORIGIN      
        1 ctacgtcgag ctgatccgca acacgccgtt tctggttcag ctcttcttct tcttcttcgc
       61 gctgccggcg atcggccttc gctggtcggc gcacaccgcg gcgctgaccg cgatggtcgt
      121 caacctgggc gcctacgcga ccgagatcat ccgcgccggc atcgaggcga tcccgaaagg
      181 gcagatcgaa gccggcctgg cgctcaacct gaaggcgcat gagatcttcc gcttcgtcgt
      241 cctcaagccg gcgctgaagg cgatctatcc ggcgctgacg agccagttca tcctgctcat
      301 gctgagctcg agcgtcgtct cggtgatctc ggccgacgac ctgacgtcgg tcgcctcgaa
      361 cctgcagtcg cagacctttc gcagcttcga gatctacatc gtcgtcgcgg ggatctacct
      421 ggcgctggcg ctgtcgttct cgaccctgtt tcgcgtcgtc taccgccacg cgttcaacta
      481 tcccgaccgt cgctaggcat gaacagagcc gatgacgcgt gtcttcgttc gctgcctccg
      541 caggctgcgt gccctcttcc ccggacgtct cgacgggact gacgcatgcg cacgttcgga
      601 cccggcgatt tcctgtacat cctcgcggcc gcgcgctgga cgctggggct ctcgctgatc
      661 gccttcgtcg gcggcgccgt cggcggcctc gccgtcgccc tcagccgcac cagcgacatc
      721 ggctggctgc gcaacgcctc ggcggccttc atccagctct tccagggcac gccgctgctg
      781 ttgcagcttt tcctcatttt cttcggcgcg ccggtgctcg ggttcgacat caacccgtgg
      841 gttgccgccg gcatcgccct cgtgctcaac agcagcgcct tcctcggcca gatctggcga
      901 ggctgcatcg aggcgatccc gcgcggccag cgcgaggccg ccgattcgct gagcctgtcg
      961 taccgcgacc ggatgcgcga cgtcatcctg ccgcaggcgc tgcggatcgc catcgcgccg
     1021 accgtcgggt acatggtgca gatcatcaag ggcacgtcgc tggcggcgat catcggcttc
     1081 accgagatca cgcgcgccgg ccagatcatc aacaacgcga ccttccagcc gctgatcgtc
     1141 ttcagcgccg tcgcggcgat ctacttcgtc ctctgctggc cgctgtcgct gctcgcggcg
     1201 ggcatcgaac gccggcaccg aagggcgctg gcgcgctgaa ctgcccacgg cgcgccgatt
     1261 gctgcatcat cgcttcatcc acgaagagga gacaacgtca tgatccgatc cccccaacgg
     1321 cgacgcgtcg caatcagcct ggcgaacgtc ggcctggccg ccgccctgcc cgcgtggctc
     1381 gtgaccgccc gggcgcagac cgtcgacgag atcaagaaaa agggcgagct caccgtcggc
     1441 ctgctggtcg actttccgcc ctacggcacg gtcaacagcg ccaacgagcc cgacggctac
     1501 gacgccgacg tcgcccgcct tctcgccaag gactggggcg tcaagctgaa gctcgttccc
     1561 gtcacggggc cgaaccggat cccgttcctg ctcaccaaca aggtcgacct gctgatcgcc
     1621 tcgctggcga tcacgcccga gcgtgccaag caggtgctgt tctcgaagcc gtacgcggcg
     1681 gcgacgatcg tcctgctcgg cagcaagaag gcccacatca aggggccggc cgacctcaag
     1741 ggcttgcgca tcggcgtcgc ccgggcgagc acgcaggaca tcgccctcac ggccatcgcg
     1801 cctcccggca ccgagatccg ccgcttcgac gacgacgcct cgggcatgca ggcgctgatg
     1861 tcgggccagg tcgacgcgct gggctgctcg accacggtgg cggcgcagat cgaaaagcgc
     1921 gcgccggcga acacctacga cgagaagttc gtcctgcgcc agcaggtgat gggcgtggcg
     1981 atgcggccgg gccagaccga gctcgagaag gcggtcaacg cgttcatcga ccggaacaag
     2041 gccaacggcg agctcgacaa gctctacgag aagtggctcg gcaccaagct gccggcgatg
     2101 taggcgagcc gatgcgggat cggagaccat gagcgacccc ggcgccgaac cgatcatcct
     2161 catcgagggg ctggacaagt ggttcggcca cttccaggtg ctgaccgaca tcgacctatc
     2221 ggtgcgcgcc ggcgagcgga tcgtcatctg cggcccttca ggctcgggca agtcgaccct
     2281 catccgctgc atcaaccgcc tcgagacggc gcaaaagggc cgcatcgtcg tcgacggcgt
     2341 cgatctcgcg gccggccggc gcaacgtcga ccgggtgcgg cgcgaggtcg gcatggtgtt
     2401 ccagcagttc aatctctttc cgcacctgac ggcgctgcag aactgcacgc tggcgccgat
     2461 gcgctcgcgc ggcatgagca aggacgaagc cgaagccacg gcgatgaagt acctgacccg
     2521 cgtgcgcatc cccgagcagg cgaacaagta tccgagccag ctctcgggag gccagcagca
     2581 acgcgtcgcg atcgcgcggg ccttgtgcat gaacccgaag atcatgctct tcgacgagcc
     2641 cacgtcggcg ctggaccccg agatggtcaa ggaggttctc gacaccatga tcggcctggc
     2701 cgaggacggc atgacgatgc tgtgcgtcac gcacgagatg gggttcgcgc gaagcgtcgc
     2761 cgatcgcgtc gtcttcatgg ccgaaggccg catcgtcgaa gaagcgccgc cggagcagtt
     2821 cttcgcccac ccacagcacg agaagacgcg caatttcctc ggccagatcc tcgtctcgca
     2881 tcacacgcga cccgagcggc tgcgcaccga gccggcgagc atcccgccgg tcgcgccggt
     2941 gttcggtccg gcaccctgat cgacgtcgac gcaagcgggg ccgatgtccg ggccctcgac
     3001 tccatgcggg tcggcacgat ggaccggtcg cctgatcgat cgtaggatcg aagtccacca
     3061 ataattcccg ggagtcattg aatgacggac gtcgttttct cgaacaaggg ggcgctttcg
     3121 aagccgtcga aagccagcgt gatgcttccg cagttccagt tccctgcctt cgaactgacc
     3181 ttcagcgaca agacggtgga ggagatgctg aagaaggaca gcaaggattg ggcgcgggct
     3241 cgcaccaaga tgggagaaga aacggccagg gagtacaagg tctacaagga ggccgtggaa
     3301 aaagggctcg ccgagctcga tgcgatgtgc aagggcaagg acaaggccac ctgcgacaag
     3361 tcggtcgcga cgttcgaggc ggtgctgaag cgggccaccg agtcggtcga cgcgagcatc
     3421 cgcaagattc cgccgcaggt ctggtccgac tggatcaaga cgcgcgacaa gtacaagaag
     3481 tatcagtaca agacggcggt ttccatcggc aagtcggtgc tgggcgtcat cagcagcgcc
     3541 gcctcgctgg ccggcgcggt gggcacctac ggcgccacgc tgccgctgtc catcatcgcg
     3601 ctgacgcgca ccagcatcgg cctggccacc gacgtcaaga acgcggcgct cgaggtcgag
     3661 catgtcgaga aagtcatccg caccgacgcc aagatcctgg aagcggcgtt caagaagggt
     3721 ggcaaggcgg ccctcgtcgc cggctcgatg ggcgtgaagg ccgtgcagtc gatcctcaac
     3781 accgacatcg gcccgagcat gaagacgctc accagcaatg tcacgctgtg gggcaacaag
     3841 tttgccggcc tcgaagtcac cgtgacccgc ctgggtggca gcgtcaccga gttcgtcgac
     3901 aagggcgagg agctcaccaa gttcatcaag acactgccgg tgggcaaaga acgaaagaag
     3961 atggaagaaa agcaggccaa gctggaaaag gcgaccgcca agctgctcag cgcgaacgac
     4021 gcgatgaacg accggatgga aaaggcgcag aagaaattca ataccaactg cgtgccggcc
     4081 atcaagacgc tgaatgcggc gtatccgaag ctggcgacga agttcgagga cctcgcgccg
     4141 gttgtcgtca gcctgggcct gggcctgggc ggcgccgact acagcgcgat ggcctccggc
     4201 tcggtggaag ggctgaaggc gatgctcgaa gtcggcaacg agctggccgg ggcgaccaag
     4261 gaggtgtatt cagccttgaa gaagtagagg cggcgcccgg cgtcgtcccg cagccggcgt
     4321 gtctctatcg ctggcacggg gccgcagcgg cgctctcggg cgcccgaatc atcgaaccgt
     4381 cgtccgatca cggccgggat cggacgcgat cgagctcatg gcatcgaagc aaggcgtctc
     4441 gcaaccccgc cttttcaaca gggaccgacg aatgccgaaa cgaaccctca tcgccctctt
     4501 cattcttgtc gacatcctgg tcatctgcgg tctgggctac gcgttcagga actacttctg
     4561 gcactactgc gagacgaacc aatgcaagag caatcccaaa ggggtctact ccgcgagcaa
     4621 gccgtccatc taggccgacg tattgccaac agcgccggcc gccgagcgac gtcgcgtcga
     4681 cgctgatccg caaggtgcca gcgccaacgc gctccggcaa gccgcgcctc gcgttgatcg
     4741 ccccgggcga aagtcgcgag agggacgcca cgccctgact tcggcagaag ggaacgacgc
     4801 aatacactcg actcgaacgt tcggctgcga atcgggtctg gccgcgtcgt tcatccaggt
     4861 gatcgcgagt ccgccaagca tgaagccaac cgatcccgcc gcgcagccga ggccgcctcc
     4921 gatcgcattt catcgggatc gcaccgcggc atgggctgct gcgctggcca tcgtggccgc
     4981 ggtcgccgag atcgcgatcg acatggccac cccgctcgaa ctcgacctcg cgtcgatcta
     5041 ccctatcccg ttgctgcttg ccgcgtatac ccgccgccgg caattgttgt ggtcactggc
     5101 actgctgctc ggattcgcca ccctcgtcgt ctacgcactc cacgccgaag ccctctcgcc
     5161 gatgctcaag ggcgaggtgc tttccaaccg gatgttcgac gtggtggcgc tcctggtcgc
     5221 caccggcgtg ctgcaccact ggatgcgctc gctcgaggtg cgcgagagcc agtccaggct
     5281 gctcggcgag cagaaccgac gcctcgagac ggccaaccgc ctcttgcgcg agcatgaggc
     5341 gcagatcctg aaacagaacg aggaactcga caggcgccac aaggatgtgg tggcgtcgag
     5401 cgcgcgcatg agccagatgc tcgtcgcggt ctcgcacgac atccgcacgc cgatccagac
     5461 catcgggctc gtggccgaac tgatgcgccg caccggcgaa gaccgggcgc tggccgaccg
     5521 cgttccgcag atggcccagc gcctgcaatc caatgcggcc acgctggtga ccatggtctc
     5581 agacctgctc gacctcgcgc gattcgatgc cggccgcacc gagctcaggc aaacgacctt
     5641 cacgctcgac gaactggtcg cttcgaaatg ccgtgacctg ggtgcgctgg cagagacgaa
     5701 atcgctggcg ctgatcgcgc gcacgccatc cgtgccgctc tgggtgcgca gcgaccgcgc
     5761 caaactcgag cgcgtgctct ccaacctggt cggcaatgcg atcaagttca cg
//
LOCUS       DASRWS010000235         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3541987, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000235 DASRWS010000000
VERSION     DASRWS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3541987"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFF72_04340"
     CDS             complement(<1..805)
                     /locus_tag="VFF72_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZV92420.1"
                     /translation="MKPVTILVTALGGEGGGVLAEWLVDTATRAGHAVQSTSIPGVAQ
                     RTGATTYYVEVFPVPERELDGRRPVFSLNPVPGALDLLASSELLETVRQVGNGMAHRQ
                     RTHVITSTSRSLTVAEKMQPGDGRVAEAELLRVVRDNALESEAFDMTAVARETGTVIS
                     AVLFGAIAASGLLPFSRADFETTIRHSERGADASLRGFAKAFDIVASMRAERRAVQDA
                     AQALARSLERPAVERDAALDAFPADVRDIIGLGCARLEDYQDAGYARLYR"
     gene            complement(802..>2177)
                     /locus_tag="VFF72_04345"
     CDS             complement(802..>2177)
                     /locus_tag="VFF72_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZV92421.1"
                     /translation="DVGLIVQGGLYNALVRALQQLGLADAFGESAISMLVLNVTFPLV
                     PDEIAAFCRDKRAVLVVEEGQPEYIERDILASLHRAGVDTALHGKDLLQMAGEYSVEV
                     LAAGLIDFAERHLPEVDIAATRDWLSGNAQRRADVAAALGGALPARPPGFCIGCPERP
                     VFSALKLAQQEVGPVHIAADIGCHALATFEPFSSGHSILGYGMSLASSAGVAPMMKRR
                     VLSVMGDGGFWHNGLLTGVQSALFNGDDSVLMIMKNGYTSATGTQDIISTPDEESKAR
                     APDKQQSLVDRNTTIESTLKGLGVQWLRTVHTYRVAEMKKTLEEAFTSEFAGLKVIIA
                     EGECQLERQRRIRPWIAGLLKRGKRVVRVKYGVDEDVCTGDHACIRLSGCPTLTLKDN
                     PDPLKVDPVATVIDGCVGCGLCGENAHAATLCPSFYRGEVVQNPRWGERAIESLRAVF
                     RRALQPA"
BASE COUNT          318 a          737 c          787 g          335 t
ORIGIN      
        1 cgcgatacag gcgggcgtag cccgcgtcct ggtagtcctc gagtcgggcg cagccgaggc
       61 cgatgatgtc gcgcacgtcg gcggggaagg catcgagggc cgcatcgcgc tcgaccgcgg
      121 gtcgctccag cgatcgcgcc agggcctgcg ccgcgtcctg cacggcgcga cgctcggcgc
      181 gcatcgaggc gacgatgtcg aaggccttcg cgaagccgcg caggctggca tcggcaccgc
      241 gttcggaatg gcggatcgtc gtttcgaagt cggcccgcga aaacggcagc aggccgcttg
      301 ccgcgatcgc gccgaagagg acggcgctga tgacggtgcc cgtttcgcgc gcgacggccg
      361 tcatgtcgaa ggcctcgctt tcgagggcgt tgtcgcggac gacgcgcagc agttcggcct
      421 cggcgacgcg gccgtcgccg ggctgcatct tctcggccac cgtgaggctg cgcgaggtcg
      481 aggtgatgac gtgggtgcgc tggcgatgcg ccatgccgtt gcccacttgt cgcaccgtct
      541 cgagcagctc cgacgacgcc agcaggtcga gcgcgccggg caccgggttg aggctgaaga
      601 ccggacggcg gccgtcaagc tcgcgctccg gcacgggaaa cacctcgacg tagtaggtgg
      661 tcgcgccggt gcgttgggcg actcccggaa tcgatgtgct ctgcacggcg tggccggccc
      721 gcgtcgccgt gtcgaccagc cattcggcga ggacgccgcc gccttcgccg ccgagcgccg
      781 tgacgaggat cgtgacgggc ttcatgccgg ctgcagggcg cgacggaaga ccgcccgcag
      841 cgactcgatc gcgcgctcgc cccagcgcgg gttctgcacc acctcgccgc gatagaacga
      901 cgggcacagc gtcgcggcat gggcgttctc gccgcacagg ccgcagccga cgcagccgtc
      961 gatcacggtg gcgaccggat cgaccttgag cgggtcgggg ttgtccttca aggtcagggt
     1021 cggacaaccc gacaggcgga tgcaggcgtg gtcgccggtg cagacgtcct cgtcgacgcc
     1081 gtacttcacg cgcaccaccc gcttgccgcg cttgaggagg ccggcgatcc acggccggat
     1141 gcggcgctgc cgctcgagct ggcattcgcc ttcggcgatg atcaccttca ggcccgcgaa
     1201 ttcgctcgtg aaggcttcct cgagcgtctt cttcatctcg gcgacgcggt aggtgtggac
     1261 ggtgcgaagc cactgcacgc ccagcccctt cagcgtcgat tcgatggtcg tgttgcggtc
     1321 gaccaggctt tgctgcttgt cgggcgcgcg ggccttgctc tcttcgtctg gcgtcgagat
     1381 gatgtcctgc gtgccggtcg cggacgtgta gccgttcttc atgatcatga gcaccgaatc
     1441 gtcgccgttg aagagcgccg attgcacgcc ggtgagaagg ccgttgtgcc agaacccgcc
     1501 gtcgcccatc accgagagga cgcgccgctt catcatcggc gcgacgcccg cgctcgaggc
     1561 caggctcatg ccgtagccga ggatcgagtg gcccgacgag aaaggctcga aggtggccag
     1621 tgcgtggcag ccgatgtcgg cggcgatgtg gaccggcccc acttcctgct gcgccagctt
     1681 cagcgccgag aacaccgggc gctccgggca gccgatgcag aacccgggcg gccgcgccgg
     1741 caatgcgccg ccgagcgcag cggcgacgtc ggcgcgacgc tgcgcgttgc cggacagcca
     1801 gtcgcgcgtg gcggcgatgt cgacctcggg cagatgccgt tcggcgaagt cgatcagccc
     1861 cgcggccagc acctcgaccg agtactctcc ggccatctgc agcaggtcct tgccgtgcag
     1921 cgcggtgtcg acgccggcgc gatgcagcga ggcgaggatg tcgcgctcga tgtactcggg
     1981 ctgcccttcc tcgacgacca ggacggcccg cttgtcgcgg cagaacgcgg cgatctcgtc
     2041 gggcacgagc gggaaggtga cgttgaggac gagcatcgag atcgcgcttt cgccgaacgc
     2101 gtcggcgagg ccgagctgct gcagcgcgcg aacgagcgcg ttgtagaggc cgccctggac
     2161 gatcagtccg acatcgc
//
LOCUS       DASRWS010000236         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_673583, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000236 DASRWS010000000
VERSION     DASRWS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673583"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFF72_04350"
     CDS             <1..238
                     /locus_tag="VFF72_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HZV92422.1"
                     /translation="LDDLTKMANGAMGALSGMKGEVENRVRDQIAKILDGMDIPRRDE
                     FEAVKAMAAKAREENEELKKQIADLQARLLNTGP"
     gene            242..2257
                     /locus_tag="VFF72_04355"
     CDS             242..2257
                     /locus_tag="VFF72_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZV92423.1"
                     /translation="MRVGVEVGGTFTDLVAVEGGRVVVTKVPSTPRSPDIGAFAALAA
                     SGIDLARIEDLGHGSTVATNAVLERKGAAVAFVATAGFRDLLFMQRHDRRNIYDLFYA
                     KPAPPVRRKDCFEVSERLRADGSVEMPLDEAKVRAELVPALQAGGYRAVAICLLNAYA
                     DPTHEKRLAALIVEALPGVLVTCSHQVAREFREFERASTTLLSAYVQPVIDGYLHRFE
                     NKLADAGFKGRFTVMQSNGGRLPAEAMRESAITALYSGPAAGVVGATRQAARSGFKDL
                     ITFDMGGTSTDVCLVQDGRPSLASESEIDGLPIRTPVLDIVSVGAGGGSIAWVDDGGM
                     LRVGPQSAGADPGPACYGRGGAEPTITDAHVMTGTIRPAAFLGGRMTLDAEAARKVFG
                     PVAERFSLGVEQAAAAALQLADANIVRAIQLVSTERGRDPRDYALVPFGGAGPLHAAR
                     IAEELGIATIVVPPNAGVISAYGLVASDYTKFDAVTRKMKLDETAAREASKSFADMRA
                     RLATQFADMKLPGELLYTHTLDMRFVGQAFEVGVEIPADRLASLDAAYLAELFADAHH
                     RTFMHGATLDRPVEIVTLRVGATLPIGMAPKLDRETVAAHESERTRIFHGEAWVDCAR
                     HTAEALTSGQQIVGPAVIEGHTATTWVPAGWTAILDAADNLILRRAS"
BASE COUNT          393 a          784 c          738 g          382 t
ORIGIN      
        1 tctcgacgac ctcaccaaga tggccaacgg cgccatgggc gcgctgtcgg gcatgaaggg
       61 cgaggtcgag aaccgcgtgc gcgaccagat cgccaagatc ctcgacggca tggacattcc
      121 gcggcgcgac gaattcgagg cggtgaaagc aatggccgcc aaagcgcgcg aggagaacga
      181 ggagttgaag aagcagatcg ccgacctgca ggctcggctt ctcaatacgg ggccgtaacc
      241 catgcgcgtc ggcgtcgagg ttggcggcac cttcacggat ctggtcgccg tcgagggagg
      301 tcgtgtcgtc gtcaccaagg tgccgagcac gccacgctcg cccgacatcg gcgccttcgc
      361 agcattggct gcctctggca tcgatctcgc gcgtatcgaa gatcttggcc acggttccac
      421 ggtcgcgacc aatgcggtgc tggagcgcaa aggcgcggcc gtcgctttcg tggcaaccgc
      481 cggctttcgt gacctgctct tcatgcagcg ccatgaccga cgcaacatct acgatctttt
      541 ttacgccaaa cccgcgcccc cggtgcgccg caaggattgc ttcgaggtca gcgaacgctt
      601 gcgcgccgac ggctcggtcg aaatgccgct cgacgaagcg aaggtgcggg ccgaactcgt
      661 tcctgcactt caggccggag gatatcgcgc tgtcgcgatc tgcctgctga acgcctacgc
      721 cgatccaacg cacgagaagc ggcttgccgc gctgatcgtc gaagcgcttc caggcgtact
      781 ggtcacctgc agccatcagg tcgcgcgcga gttccgcgag ttcgaacgcg cctcgacgac
      841 gctgctctcg gcctatgtcc agccggtgat cgacggctac ctgcaccgct tcgagaacaa
      901 gctcgccgac gcaggcttca agggccgctt caccgtgatg cagtcgaacg gcggtcggct
      961 gcccgcagag gcgatgcgcg agagcgcgat cacggcgctt tattccggac cagccgccgg
     1021 cgtcgtcggc gccacacgcc aggccgcgcg ctcaggcttc aaggatctga tcactttcga
     1081 catgggcggc acttcgaccg atgtctgcct ggtccaggac ggacggcctt cgctcgcctc
     1141 ggaaagcgag atcgacggtc tgccgatccg tacgccggtg ctcgacatcg tttcggttgg
     1201 tgcgggcggt ggctcgatcg cctgggtcga cgacggcggc atgctgcggg tcggcccgca
     1261 aagcgcgggc gccgatccgg gccccgcctg ctacggccgc ggcggcgccg agcccaccat
     1321 caccgatgcc catgtcatga cgggcacgat ccggccggcg gcgttcctgg gcggacgcat
     1381 gacactcgac gctgaggcgg cgaggaaggt cttcgggcca gtggcggaac gcttcagcct
     1441 cggcgtcgag caggcggcgg ccgcggcgtt gcagctcgcc gacgccaata tcgtgcgcgc
     1501 aattcagctc gtctcgaccg aacgcggccg cgacccgcgt gactatgccc tggtgccgtt
     1561 cggcggtgcc ggtcccctgc atgccgcgcg cattgccgag gaactcggca tcgccaccat
     1621 cgtcgtgccg ccaaacgctg gggtgatctc cgcctacggc ctggtcgcct ccgactacac
     1681 caagttcgat gccgtcaccc ggaagatgaa gctcgacgaa acggcggccc gtgaggcgag
     1741 caagtccttc gccgacatgc gcgccaggct cgccacgcaa ttcgccgaca tgaagcttcc
     1801 tggtgaactt ctgtacaccc acacgctcga tatgcgcttc gtcggccagg ccttcgaagt
     1861 cggtgtcgag attccggccg atcggctcgc ctcgctcgat gccgcttacc tcgccgagct
     1921 gttcgccgat gcgcatcatc gcaccttcat gcatggtgcg acgctcgacc ggccggtcga
     1981 gatcgtgacc ctgcgcgtgg gcgccacact gccgatcggg atggcaccca aactggaccg
     2041 cgagactgta gctgcgcacg agtccgaacg aacccgcatc ttccacggcg aagcctgggt
     2101 tgactgcgca cgccataccg ccgaggccct gacaagcggc cagcagatcg tcggaccagc
     2161 cgtgatcgaa ggtcacactg ccacgacctg ggtaccggcg ggctggaccg caatactcga
     2221 cgcggcggac aatctcatcc tgcggagggc atcatgagcg tcgtgctcac tccccttgat
     2281 tatgcggtga tcagcca
//
LOCUS       DASRWS010000237         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_959557, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000237 DASRWS010000000
VERSION     DASRWS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959557"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFF72_04360"
     CDS             complement(<1..608)
                     /locus_tag="VFF72_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZV92424.1"
                     /translation="MRHIDDALVDELIGPVDAQQALLEAFRSFGAGRAAMQERMRTES
                     GGVKLSTLGAVMPDQGFVGAKVYTTITGQFRFVILLFDAADGRPLASFDAGAITRLRT
                     AATSVIASRYLARTDAKTMALFGAGVQGREHAQQMAATYPLTELRVCDPFAATEVFTD
                     LERRCGVTVRRCGNGAEALAGADIVVTASRAKTPLFNGDRLP"
     gene            complement(631..1590)
                     /locus_tag="VFF72_04365"
     CDS             complement(631..1590)
                     /locus_tag="VFF72_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZV92425.1"
                     /translation="MNLFSKLVVLAAVAMSAATATAQTYPDKPVRVVVTTVPGPLDAF
                     ARVVLQQIEKRLKQTFIVDNRAGAGGNLGADIVAKSPADGYTLLFALDTTFTVNPWLY
                     RKMPFDVTKDFTIISVPVTYSQALAVNPNVKATSVAEFVNLAKSTHMSYASGGNGSPS
                     HLTMAAFMAAAGINMTHIPYKGTGASVIDVMGGQVDSLFAVGSGILPHAKAGKLRALA
                     VSGLTRSAAAPELPTIAESGYPGFNASFAYVLAAPAGTPDEIVQLLAREVALAMNTPQ
                     VRELDRQADYAATNLTQAQSGAWLAEMRQHWGTVVQKSGITIE"
     gene            complement(1593..2594)
                     /locus_tag="VFF72_04370"
     CDS             complement(1593..2594)
                     /locus_tag="VFF72_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV92426.1"
                     /translation="MGYRIEIQETGEIFEAGDGEAVLDAAMRQGIGLRHECTLGTCGT
                     CRVRLLEGSVRYDDVPPALSKAEAEQGFALMCQAHATSALVISTARTLATVAEPSRHA
                     AVIKEVRPLTSEVLHLKLGIAGLDAPNWRPGQYMNVHLPDGSHRSFSMASCPGSDSVD
                     FHVRRIAGGRFTDARLAAMRPGERLDIEFPLGPFGLHKADYRPLLMVATGTGIAPIKS
                     MLESLLDDDECPPVWLYWGMRTEADLYLDEVFRSWQGRLYEFQYVPVLSRADASWRGR
                     RGHVQQAAIADLPDLSEHGIYLCGSPAMIDEAKNEFVAHGAQLDHLHVDAFTFQQES"
BASE COUNT          423 a          863 c          884 g          436 t
ORIGIN      
        1 ctcggcagcc ggtcgccgtt gaacaagggc gtcttcgcgc gcgacgcggt gaccacgatg
       61 tcggcgcctg caagggcttc agcgccattg ccgcagcgtc gcaccgtgac gccgcatcga
      121 cgctcgagat cggtaaacac ctcggtcgcc gcgaacgggt cgcagacgcg caactccgtc
      181 agcgggtatg tcgcggccat ctgctgcgcg tgctcccggc cttgcacacc ggctccgaac
      241 agggccatcg tcttcgcatc cgttcgcgcc aggtagcgac tggcgatgac cgaggttgcc
      301 gccgtgcgca gccgtgtgat cgccccggcg tcgaacgacg ccagcggtcg gccgtcggcg
      361 gcatcgaaca acaggatgac gaagcggaac tggcccgtga tcgtcgtgta gaccttcgcc
      421 cccacgaagc cctggtcggg catgaccgcg ccgagcgtcg aaagcttgac gccgcccgac
      481 tcggtgcgca tgcgctcctg catcgccgcg cgacccgcgc cgaagctgcg gaacgcctcg
      541 agcaacgcct gttgggcatc gaccgggccg atcagctcgt cgaccagcgc gtcgtcgatg
      601 tgccgcatgc cgccgccggg tgccgaacgc tcactcgatc gtgattcccg acttctgcac
      661 caccgtgccc cagtgctggc gcatctcggc caaccaggcg ccggactgtg cctgcgtcag
      721 gttggtcgcc gcgtagtcgg cctggcgatc cagctcgcgg acttgcggcg tattcatcgc
      781 caacgcgacc tcgcgcgcca gcaactgcac gatctcgtcc ggtgtgccgg ccggcgcggc
      841 gagcacgtag gcgaagctgg cgttgaagcc cggatagccg ctctcggcga tcgtcggcag
      901 ttccggcgcg gcggccgatc gggtcagtcc cgacaccgca agcgcgcgca gcttgcccgc
      961 cttggcatgc ggcaggatgc cgcttccgac ggcgaacagc gagtcgacct ggccgcccat
     1021 cacgtcgatc accgacgcgc cggtgccctt gtacggaata tgggtcatgt tgatgcccgc
     1081 ggccgccatg aaagcggcca tcgtcagatg gctgggcgag ccgttgcccc ctgaggcgta
     1141 gctcatgtgc gtcgacttcg cgaggttcac aaattcggcg acgctggtgg ccttgacgtt
     1201 cgggttcacc gccagcgcct ggctgtaggt gactggaacc gagatgatgg tgaaatcctt
     1261 cgtcacgtcg aagggcatct tgcggtagag ccacggattg accgtgaacg tcgtgtcaag
     1321 tgcgaacagc agggtgtagc catcggcagg agacttggcc acgatgtcgg cgccgaggtt
     1381 gccgccggca ccggcacggt tgtcgacgat gaacgtctgc ttcaggcgct tctcgatctg
     1441 ttgcaggacg acccgggcga aggcgtcgag cggcccaggg acggtggtga cgacgacacg
     1501 aaccggcttg tccggatagg tctgtgccgt cgcggtggcg gccgacattg ccacggccgc
     1561 gaggaccacc agtttgctga agagattcat catcaagact cctgctgaaa agtgaacgcg
     1621 tcgacgtgaa gatggtcgag ttgcgctcca tgggccacga attcgttctt cgcttcgtcg
     1681 atcatggcgg gcgatccgca caggtagata ccgtgctcgg acaggtcggg aaggtcggcg
     1741 atcgcggctt gctgcacgtg gccacgccgg ccgcgccacg acgcatcggc gcgcgagagc
     1801 accggaacgt actgaaattc gtagaggcgc ccctgccacg aacgaaacac ctcgtcgagg
     1861 tagagatcgg cctcggtccg catgccccag tacagccaga ccggcgggca ttcatcgtcg
     1921 tcgagcagcg actcgagcat gctcttgatc ggcgcgatgc cggtgcccgt ggcgaccatc
     1981 agcagcggcc ggtagtcggc cttgtgcagg ccgaaggggc cgagcgggaa ctcgatgtcg
     2041 agcctctcgc ccgggcgcat cgccgccagt cttgcgtcgg tgaagcgacc gccggcgatg
     2101 cgcctcacgt ggaagtcgac gctgtcgctg ccagggcacg atgccatcga gaagctgcga
     2161 tgcgagccgt cggggaggtg cacgttcatg tactggccgg gccgccagtt cggcgcatcg
     2221 agtccggcga tcccgagctt caggtgcagc acttcggaag tcagcggccg aacctccttg
     2281 atgacggccg cgtgtcgcga cggttcggcg acggtcgcga gggtgcgggc cgtgctgatg
     2341 accagcgcgc tcgtggcatg ggcctggcac atcagcgcga agccctgctc ggcctcggcc
     2401 ttgctcaacg ccggtggcac gtcgtcgtag cggacgctgc cctcgagaag cctgacgcgg
     2461 caggtgccgc atgttccgag ggtgcattca tggcgcaggc caatgccctg acgcatcgcg
     2521 gcgtcgagaa cggcctcgcc gtccccggcc tcgaagatct cgccggtctc ctgtatctcg
     2581 atccgatagc ccatgaccgg tgggct
//
LOCUS       DASRWS010000238         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2384549, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000238 DASRWS010000000
VERSION     DASRWS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384549"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFF72_04375"
     CDS             complement(<1..699)
                     /locus_tag="VFF72_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HZV92427.1"
                     /translation="MEVSFSKEVEQLRLGAGDVFHGEGILAVTKALLQSGVAYVGGYQ
                     GAPVSHLLDVMVQAEPYMKELGVHVEACANEASAAAMLGASIHYPLRGAVTWKSIVGT
                     NVAADALSNLSSPGVTGGALIVVGEDYGEGASVIQERTHAYAMKSSMLMLDPRPELGN
                     LVRMVEEGFRLSEASNMPAMLELRIRACHVRGSFVCKDNVAPRVSTMQPLAEPARFDY
                     ARLAHPPVTFGHEKK"
     gene            815..1729
                     /locus_tag="VFF72_04380"
     CDS             815..1729
                     /locus_tag="VFF72_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZV92428.1"
                     /translation="MHIREIDLNLLRVFDSVHRLRSVSRAADALGLSQPAVSQSLSRL
                     RLLMKDALFTRVAGGVLPTASADALAVSVGEAMALLEQGLHGSASFEPAQARRTFRVH
                     MSDIGEAEFLPGLMRRIRAEAPDVRIETHQLDYAAIEDALDSGKVDVAFGYLPGVEKT
                     EHQRLFMERYVVLGRADHPVLTARPSHAVLGRLDYVVVRHHTETARVLERLSLTANVR
                     LSIPHFMVIPAIVGATNLAVVLPLRIARKFATDGRCRIARPRWTIDDIPIALHWSRRA
                     RADPGIRWLRQLTSELFLETPRRIATRD"
     gene            1751..2956
                     /locus_tag="VFF72_04385"
     CDS             1751..2956
                     /locus_tag="VFF72_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZV92429.1"
                     /translation="MPAMPLRAIAFLLYAYVGWRIAPALADRWLAGGFIAVLFASALT
                     LPHAFRARRVERPRPLDRALAWIGLVGMGLFSSLFVLTVLRDLGLVLVAGFAAWRPAD
                     VSFARVALLSARAVPVLAALVTAWGFLNARRTASVVRVDVPIAGLPAALQGFTIAQIS
                     DVHVGPTIRRDYLEAIVVVVNRLDADLVAVTGDLVDGSVADLAEHVAPLAELRSREGT
                     FFVTGNHEYYSGAAPWVRELRRLGLKVLMNEHVVVRRGDASLVVAGVTDFGAGHFDGS
                     QASDPAGAAAGSPPDAALRVLLAHQPRSAPAAEAAGFDLQLSGHTHGGQFLPWNFLVR
                     LQQPFTAGLHRWRGMWVYTSRGTGYWGPPKRIGAPSEITLLRLVAGAGPAAADRTSRQ
                     RALPAATPA"
     gene            3085..3360
                     /gene="yajC"
                     /locus_tag="VFF72_04390"
     CDS             3085..3360
                     /gene="yajC"
                     /locus_tag="VFF72_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZV92430.1"
                     /translation="MFGSIGSMLPLVLMFVVLYFVMIRPQMKRQKETKAMIEALAKGD
                     EIVTSGGMLGKISKIGESFLSVEVGNGVEIQVQRSAVVQVMPKGTLK"
     gene            3379..>4796
                     /gene="secD"
                     /locus_tag="VFF72_04395"
     CDS             3379..>4796
                     /gene="secD"
                     /locus_tag="VFF72_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZV92431.1"
                     /translation="MNRYPAWKYAILAVALVIGIVYTLPNFFGEAPAVQISSGKATLK
                     LDASLETTVTQILERAGLKPDFVEFDGNSVKARFDDLDTQRKAQDALIATLNPDPSNA
                     SYIVALNLLSRSPRWLTAIHALPMYLGLDLRGGVHFLMQVDMKSALTKKAEALTGDIR
                     TLLRDRDIRHSGITRDGDDVTIRFRDEATRTAAKNLLQDQLPDLLWQDESQGGDFALT
                     GALKADAAKRVQEAAITQNITTLHNRVNELGVAEPVIQQQGADRVVVELPGVQDTAKA
                     KDIIGRTATLEMRMVDDSPEARAAETGAGPVPFGDERYVERNRAPIIVKRQVIITGEN
                     LTDAQPGFDENHQPAVHLTVDAKGARIMRDISRDNIGKRMAILLFEKGKGEVVTAPVI
                     RGELGNRFQISGAMTTSEANDTALLLRAGSLAAPMEIIEERTIGPSLGAENIAKGFHS
                     VMYGFAAIAVFMCSYYLLFGVIS"
BASE COUNT          722 a         1706 c         1617 g          751 t
ORIGIN      
        1 cttcttctcg tgaccgaagg tgacgggagg atgggcgagc cgcgcatagt cgaagcgcgc
       61 cggctcggcc aggggctgca tcgtcgagac gcgcggcgcg acgttgtcct tgcagacgaa
      121 gctgccgcgc acatggcagg cgcggatgcg cagctcgagc atcgccggca tgttcgacgc
      181 ctcggacagg cgaaaccctt cctcgaccat gcgcaccagg ttgccgagct ccggacgcgg
      241 gtcgagcatc agcatcgacg acttcatcgc ataggcgtgg gtgcgctcct ggatcacgct
      301 ggcaccctcg ccgtagtcct cgccgacgac gatcagcgcc ccgccggtga cgcccggcga
      361 cgacaggttc gagagcgcat cggccgcgac gttggtgccg acgatcgatt tccaggtcac
      421 cgcgccgcgc agcggatagt ggatcgaagc gccgagcatg gccgccgccg aagcctcgtt
      481 ggcgcaggcc tcgacgtgca cgccgagctc cttcatgtag ggctcggcct ggaccatcac
      541 gtcgaggagg tgcgagaccg gcgcgccctg gtagccgccg acataggcga cgcccgattg
      601 cagcagcgcc ttcgtcaccg cgaggatgcc ttcgccatgg aagacgtcgc cggcgcccag
      661 gcgcagttgc tcgacttcct tgctgaacga cacttccacg cgacgactcc gcaggcggtc
      721 ggccggggcc gcgacgaccc cttccggatc gacgagctta tcggcggcga cgccgattca
      781 ccatcgacag gactgaataa gtaatattcg cgggatgcat atccgcgaga tcgatctcaa
      841 cctgctgagg gttttcgaca gcgtccaccg gctgcgcagc gtcagccggg ccgccgatgc
      901 gctcggcctg tcgcagccgg cggtgagcca gtcgctgagc cggctgcgcc tgctgatgaa
      961 ggatgcgctc ttcacccgcg ttgccggcgg cgtgctgccg accgcgtcgg ccgacgcgct
     1021 ggccgtgtcg gtgggcgagg cgatggcgct tctcgagcag ggcctgcacg gctcggcgag
     1081 cttcgagccg gcgcaggcga ggcggacgtt tcgcgtgcac atgagcgaca tcggcgaggc
     1141 cgagttcctg ccgggcctga tgcgccgcat ccgcgccgaa gcgccggacg tgcgcatcga
     1201 gacgcaccag ctcgactacg ccgcgatcga ggatgcgctc gacagcggca aggtcgacgt
     1261 cgcgttcggc tacctgccgg gcgtcgagaa gaccgagcat cagcgcctct tcatggagcg
     1321 ctacgtcgtt ctcggccgcg ccgaccatcc ggtgctgacc gcgcggccgt cgcacgcggt
     1381 gctggggcga ctcgactatg tcgtcgtgcg ccaccacacc gagaccgccc gcgtgctcga
     1441 gcggctgtcg ctcaccgcca acgtacgctt gagcattccg cacttcatgg tcattccggc
     1501 gatcgtcggc gcgaccaacc tcgccgtggt gctgccgctt cggatcgccc gcaagttcgc
     1561 gaccgatgga cgctgccgga tcgcccggcc gcgctggacg atcgacgaca tccccatcgc
     1621 gctgcactgg agccggcggg cccgcgccga tccgggcatc cgctggctgc gccagctgac
     1681 cagcgagctc ttcctcgaga cgccacgccg catcgcgacc agggattgaa gcgcggcttc
     1741 gcgctcccac atgccggcga tgccgcttcg cgcgatcgcg ttccttctct acgcctacgt
     1801 cggttggcgg atcgcgccgg ccttggccga ccggtggctg gccggcgggt tcatcgccgt
     1861 ccttttcgct tcggcgctga cgttgccgca cgcgtttcgc gcccgccggg tcgagcgccc
     1921 gcgcccgctc gaccgcgccc tggcctggat cggcctggtc ggcatgggcc tcttctcgtc
     1981 gctgttcgtc ctgacggtgc ttcgcgacct cggcctcgtt ctcgtcgccg ggttcgcggc
     2041 gtggcgaccg gccgacgttt cgtttgctcg cgtcgccttg ctcagcgctc gcgccgtgcc
     2101 ggtgctggct gcgctcgtca cggcctgggg ctttctcaac gcccggcgga ccgcgtccgt
     2161 cgttcgggtc gacgtgccga tcgccggcct gccggccgcg ctgcagggct tcacgatcgc
     2221 ccagatcagc gacgttcatg tcgggccgac catccggcgc gactatctcg aggcgatcgt
     2281 ggtcgtcgtc aaccgcctcg acgccgacct cgtcgccgtc accggcgatc tcgtcgacgg
     2341 ctcggtcgcc gacctcgccg agcacgtggc gccgctggcc gagctgcgat cgagggaggg
     2401 gaccttcttc gtcaccggca accacgagta ctactcgggg gccgctccct gggtgcgcga
     2461 gttgcgtcgg ctcggcctca aggtgctgat gaacgagcac gtcgtggtcc gccgcggcga
     2521 cgccagcctg gtcgtcgccg gcgtcaccga tttcggcgct ggccacttcg acggctccca
     2581 ggcgagcgat ccggccggcg ccgccgcggg cagcccgccg gatgcggcat tgcgcgtgct
     2641 gctcgcgcac cagccgcgca gcgcgccagc cgccgaagca gcgggcttcg acctgcagct
     2701 gtcggggcac acgcacggcg gccagttcct gccgtggaac ttcctggtcc gcctgcagca
     2761 gccgttcacc gccggcctgc accgctggcg agggatgtgg gtctacacga gccgcggcac
     2821 cggctactgg ggcccgccca agcggatcgg tgctccgtcg gagatcacgt tgctgcgcct
     2881 cgtggccggt gccggccccg cagcggccga ccggacgagc cggcagcgcg ccctgccggc
     2941 ggcgacgccg gcctgagcgg cggcggccct ctacaatcca caggttttgc cggccgtcgc
     3001 cgcgccttct tgcgaggacc ctcccccgtg tcgttcatta ccgaagccca cgcccaggct
     3061 gcgcctgccg ccggcgccga atcgatgttc ggaagcatcg gcagcatgct gccgctggtt
     3121 ctgatgtttg tcgtcctcta cttcgtcatg atccgtccgc agatgaagcg gcagaaggag
     3181 acgaaggcga tgatcgaggc cctcgccaag ggcgacgaga tcgtcacctc gggcggcatg
     3241 ctcggcaaga tctcgaagat cggcgagtcg ttcctgagcg tcgaggtcgg caacggcgtc
     3301 gagatccagg tgcagcgctc ggcggtcgtg caggtgatgc ccaagggcac cttgaagtag
     3361 gccggcggag caccgcgcgt gaaccggtat ccggcctgga agtacgcgat cctcgcggtc
     3421 gccctcgtca tcggcatcgt ctacacgctg ccgaatttct tcggcgaggc gccggcggtg
     3481 cagatctcga gcggcaaggc aacgctcaag ctcgacgcgt cgctcgagac gacggtcacg
     3541 cagattctcg agagggccgg cctgaagccc gatttcgtcg agttcgacgg caactcggtg
     3601 aaggcgcgct tcgacgacct cgatacccag cgcaaggcac aggacgcgct gatcgcgacg
     3661 ctgaatcccg atccgagcaa cgccagctat atcgtcgccc tcaacctgct gtcgcgttcg
     3721 ccgcgctggc tgacggccat tcacgcgctg ccgatgtacc tcggcctcga tctgcgcggc
     3781 ggcgtgcact tcctgatgca ggtcgacatg aagtcggcgt tgacgaagaa ggccgaggcg
     3841 ctgaccggcg acatccgcac cctgctgcgc gacagggaca tccgccactc cggcatcacc
     3901 cgcgacggcg acgacgtgac gatccgcttt cgcgacgagg cgacccggac cgcggcgaag
     3961 aacctgctgc aggaccagct gcccgacctg ctgtggcagg acgaatcgca gggcggagac
     4021 ttcgccctca ccggcgcgct caaggccgac gccgccaagc gggtccagga agcggcgatc
     4081 acgcagaaca tcacgacgct gcataaccgg gtcaacgagc tcggcgtcgc cgagccggtg
     4141 atccagcagc agggcgccga ccgcgtcgtc gtcgagctgc ccggagtgca ggacaccgcc
     4201 aaggccaagg acatcatcgg ccgcaccgcg accctcgaga tgcgcatggt cgacgacagc
     4261 cccgaggcgc gcgccgccga gacaggtgcc gggccggtgc cgttcggcga cgagcgttac
     4321 gtcgagcgca accgggcgcc catcatcgtc aagcgccagg tcatcatcac cggcgagaac
     4381 ctgaccgacg cccagccggg cttcgacgag aaccaccagc cggcggtgca cctgacggtc
     4441 gacgccaagg gcgcccgcat catgcgcgac atctcgcgcg acaacatcgg caagcgcatg
     4501 gcgatcctgc tcttcgagaa gggcaagggc gaggtcgtga ccgcgccggt gatccgcggc
     4561 gagctcggca accggttcca gatttccggc gccatgacga cgagcgaggc caacgacacc
     4621 gcgctgctgc tgcgggccgg ctcgctggcg gcgcccatgg agatcatcga ggagcgcacg
     4681 atcggcccga gcctgggcgc cgagaacatc gccaagggct ttcacagcgt catgtacggt
     4741 ttcgccgcga tcgccgtctt catgtgcagc tactacctgc tgttcggcgt gatctc
//
LOCUS       DASRWS010000239         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_674425, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000239 DASRWS010000000
VERSION     DASRWS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674425"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFF72_04400"
     CDS             <1..205
                     /locus_tag="VFF72_04400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZV92432.1"
                     /translation="TGWATGPHLHFEFKVDGEQQNPLLVAQNSQGTVLSPEAETRLTQ
                     LSASARAQLGIARTLAHAQIPGE"
     gene            217..1374
                     /locus_tag="VFF72_04405"
     CDS             217..1374
                     /locus_tag="VFF72_04405"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZV92433.1"
                     /translation="MSTAFIGLMSGTSLDGIDGVLAEFGAFGGDAAAPRPLRVLAHTH
                     AGFDPALRDELLALNRSGPDEIHRVALAANSLGRAYAGVVASLLVAAGVDRGDIAAIG
                     CHGQTVRHRPGEFDGTGYTVQINAPALLAELTGIDVVADFRSRDVAAGGQGAPLVPAF
                     HRAMFSRPGQASAVLNLGGIANLTAVAADGSVSGFDCGPGNTLLDRWSAASTGHPFDA
                     DGAWAAGGHVDDRLLRVLLREPYLSRRPPKSTGRDLFDERWLETRLVDAEARSRLRPQ
                     DIQATLAELTARTCADALDACGSAAGEVVVCGGGTRNADLMRRLSRAVAPRTVVSSDD
                     RGLPADQVEAAAFAWLARAFVGREAGNLASVTGAAGPRVLGALYPGRSPST"
     gene            complement(1429..1806)
                     /gene="erpA"
                     /locus_tag="VFF72_04410"
     CDS             complement(1429..1806)
                     /gene="erpA"
                     /locus_tag="VFF72_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZV92434.1"
                     /translation="MSAVAENIISSPEADVPPAPIVFTDSAANKVKELVDEEGNPDLK
                     LRVFVQGGGCSGFQYGFTFDEIVNEDDTQMSKNGVTLLIDAMSLQYLMGAEIDYKDDL
                     QGAQFVIKNPNATTTCGCGSSFS"
     gene            complement(1916..>2160)
                     /gene="rpsI"
                     /locus_tag="VFF72_04415"
     CDS             complement(1916..>2160)
                     /gene="rpsI"
                     /locus_tag="VFF72_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZV92435.1"
                     /translation="PLMLTANDAAFDVKVNVHGGGETGQAGAVRHGITRALIDYDVAL
                     KGELSRAGFVTRDAREVERKKVGLHGARRRKQFSKR"
BASE COUNT          319 a          761 c          704 g          376 t
ORIGIN      
        1 gaccggctgg gcgaccgggc cgcaccttca cttcgagttc aaggtcgacg gcgagcagca
       61 aaatcccttg ctcgtcgcgc agaattcgca aggtaccgtc ctttcgcccg aagccgagac
      121 gcgcctgacg cagctttcgg caagcgctcg cgcccagctc ggcatcgctc gcaccctggc
      181 ccacgcccag atccccggcg aataggcagc ggtgcgatga gcaccgcctt catcggcctg
      241 atgtcgggca cctcgctcga cggcatcgac ggcgtcctgg ccgaattcgg ggcattcggc
      301 ggcgacgcgg ctgcgccccg gccgctgcga gtcctggccc acacgcacgc cgggttcgat
      361 ccggcgcttc gcgacgagct gctggccctc aatcgcagtg gccccgacga gatccatcga
      421 gtcgccctcg ccgccaattc actcgggcgc gcctacgccg gcgtcgtcgc gtcgctgctc
      481 gtcgcagccg gagtcgatcg cggcgacatc gctgccatcg gctgtcatgg ccaaacggtt
      541 cgccaccgcc ctggtgaatt cgacggcacg ggctataccg tccagatcaa cgcgccggcg
      601 ctgctcgccg aattgacggg catcgacgtc gttgccgact tccgcagccg cgacgtcgcc
      661 gccggcggcc agggcgcacc gctcgtgccg gcgtttcatc gtgcgatgtt cagtcgcccg
      721 ggccaggcga gcgccgtcct caacctgggc ggtatcgcca atctcactgc cgtcgccgcc
      781 gacggctcgg tatcgggttt cgactgcggc cccggcaata ccctgctcga tcgctggtcg
      841 gcggcgtcga ccggtcatcc cttcgacgcg gacggcgcat gggccgcggg cggccacgtc
      901 gacgaccggc tgcttcgcgt ccttctccgc gagccgtacc tctcacgtcg gccgccgaaa
      961 agcaccggac gtgacctgtt cgacgagcgc tggctcgaga cccgcctcgt cgacgccgag
     1021 gcacggagcc ggctgcgtcc gcaggacatc caggcgacgc tcgccgagct gacggcgcga
     1081 acctgtgccg acgcgctcga cgcctgcggg tcggcggccg gcgaagtcgt cgtctgcggg
     1141 ggtggaacgc gaaacgccga cctcatgaga cgcctgtctc gcgccgtcgc gccgcgcacg
     1201 gtggtctcga gcgacgaccg aggcctgccg gccgaccagg tggaagccgc cgcgttcgcg
     1261 tggctggcac gcgcattcgt cggccgcgaa gcgggcaatc tggcctcggt caccggggct
     1321 gccgggcctc gcgtcctggg tgccctctat ccgggcagga gtccatcgac ctgacgcggc
     1381 ttccatgaaa aaggccgcca cgcggcggcc ttgtcggaat cgaaacggtc aggaaaaact
     1441 ggaaccgcat ccgcaggtcg tcgttgcgtt cgggttcttg atgacgaact gggcgccttg
     1501 cagatcgtcc ttgtagtcga tctctgctcc catcaggtac tgcaggctca tcgcgtcgat
     1561 gaggagcgtc acaccgttct tggacatctg ggtgtcgtct tcgttgacga tctcgtcgaa
     1621 ggtgaagccg tactggaacc ccgaacaacc gccgccttga acgaacacgc gcaacttgag
     1681 gtcggggttg ccttcctcgt cgaccagttc cttcaccttg ttggcggcac tgtcggtgaa
     1741 gacgatcggc gccggcggca cgtcggcttc gggagaggag atgatgtttt cggcgactgc
     1801 gctcatgcga cgctcctgcg atagggactc tttcgattat cgcagttggg ggatttccgc
     1861 gtgaatgcaa gaaagccgcc cgcaggcggc ttctgtggca ggaatttgcc gcgcttcagc
     1921 gcttgctgaa ctgcttgcgc cggcgagcac cgtgcaggcc gaccttcttg cgctcgacct
     1981 cgcgcgcgtc gcgcgtcacg aaaccggccc gactgagctc gcccttcagg gccacgtcgt
     2041 agtcgatcaa ggcccgcgtg atgccgtgcc gcacggcgcc ggcctggccg gtttcgccgc
     2101 ccccgtgaac gttcaccttg acgtcgaacg cggcatcgtt cgccgtcagc atcagcggct
//
LOCUS       DASRWS010000240         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4123790, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000240 DASRWS010000000
VERSION     DASRWS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123790"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFF72_04420"
     CDS             <1..324
                     /locus_tag="VFF72_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZV92436.1"
                     /translation="KYFTDVVRYECPCDLPPGEEEEIRRLVLAAYRTLGCRGWGRADL
                     MLRRVDRKPFLLEMNTSPGMTSHSLVPISARAAGISYEQLCVEIIAMAALDSNRPAAA
                     ASPRA"
     gene            330..1145
                     /locus_tag="VFF72_04425"
     CDS             330..1145
                     /locus_tag="VFF72_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZV92437.1"
                     /translation="MARAAFLSSGSEPPDVRFMNATASAFAVIAVLLIASAALLWLSR
                     QPFFAIRVIRIEGDVTHNSASTIRANAAPRLAGNFLTIDLGAVRRAFEAVPWVRHAVV
                     QRVWPNRLRVRLEEHRPVALWGGGDANADELVNSFGEVFEANVGDVEDDALPTLEGPD
                     GSSAHVLAMLQRLDPVFAPLRARIDALSLSSRGSWKATLDNGAVVEIGRGDDDAIVAR
                     TARFVATVGQVTGRYERPLEFADLRHTDGYAVRLKGVSTTTPAGTGDRAAAKK"
     gene            1238..>2452
                     /gene="ftsA"
                     /locus_tag="VFF72_04430"
     CDS             1238..>2452
                     /gene="ftsA"
                     /locus_tag="VFF72_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZV92438.1"
                     /translation="MAKEGKDLVVGLDIGTAKVMAVVAEVMPDGELRVAGLGSAPAHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKITRVYTGITGSHIRGQNSTGMVIVRDKE
                     VTPVDVTRVVETAKAINIPNDQRLLLVEPQEFVIDGHEVKEPIGMSGGRLEVKVHIVT
                     GAQSAAENIVKCVRRCGLEVDQLVLNPSASSHAVLTDDEKDLGVALVDIGAGTTDVAI
                     FTDGSIRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVEYGVAKQLLADPNEQLEVP
                     GLGDRPPRMLSRQALAGVIEPRIEEIYSLVHQVIRESGFEELLSSGVVITGGAAVMPG
                     MVELGEDIFLKPVRKGTPTYRGALHDMVANPRSATVMGLLEEARLARTRGQRAAQQAG
                     SVQTLFGRARDWF"
BASE COUNT          389 a          857 c          850 g          356 t
ORIGIN      
        1 aagtatttca ccgacgtcgt ccgctacgag tgtccctgcg acctgccgcc cggcgaggaa
       61 gaggagatcc ggcgcctcgt gctggccgcc taccgcacgc tcggttgtcg cggctggggg
      121 cgcgccgacc tgatgctgcg ccgtgtcgac cgcaagccct tcctcctcga gatgaacacc
      181 tcgccgggaa tgacgtccca ctcgctggtg ccgatatcgg cgcgtgccgc cggcatctcc
      241 tatgagcagc tctgcgtcga gatcatcgcc atggccgccc tcgactcgaa tcgtccggct
      301 gcggccgcga gcccgagggc atgacgacga tggcccgcgc cgccttcctt tcctccggat
      361 ccgagccgcc cgacgtgcgc ttcatgaacg cgaccgcgtc ggccttcgcg gtgatcgccg
      421 tgctgctgat cgcatcggcg gcgctgctgt ggctgtcgcg ccagcccttc ttcgcgatcc
      481 gcgtcatccg catcgaaggc gatgtcaccc acaacagcgc ctcgacgatc cgggccaacg
      541 ccgcgcctcg cctggccgga aacttcctga ccatcgacct gggcgcggtg cgccgcgcct
      601 tcgaggcggt gccctgggtg cggcatgcgg tggtccagcg cgtctggccg aaccggctgc
      661 gcgtgcgcct cgaggagcac cgcccggtgg cgctctgggg cggcggcgac gcgaacgccg
      721 acgagctcgt caacagcttc ggcgaggtct tcgaagccaa cgtcggcgac gtcgaggacg
      781 atgcgctgcc gacgctcgag ggcccggacg gcagttcggc gcacgtgctg gcgatgctcc
      841 agcgcctcga cccggtcttc gcgccgctga gggcccgcat cgacgcgctg tcgctgtcga
      901 gccgtggctc ctggaaggcg acgctcgaca acggcgccgt cgtcgagatc ggtcgcggcg
      961 acgacgacgc catcgtcgcg cgcacggcac ggttcgtcgc gacggtgggc caggtgaccg
     1021 gccgctacga gcggccgctc gagttcgcgg acctgcgtca taccgacggc tacgcggtcc
     1081 gcctgaaggg cgtgtcgacg acgacgccgg ccggcaccgg cgatcgcgcc gcagcaaaga
     1141 aataggagaa cggccatgaa ccgaccccac gctcatcgtc tccatcgcgt cgccaaggcg
     1201 ccgccggcac tcgcagcacg gcaggacggg cgctgacatg gccaaggagg gaaaggatct
     1261 cgtcgtcggc ctggacatcg gcaccgccaa ggtgatggcc gtcgtcgccg aggtgatgcc
     1321 cgatggcgag ctgcgcgtcg ccggcctcgg cagcgcgcct gcgcatggcc tgaagcgcgg
     1381 tgtcgtcgtc aacatcgatg cgaccgtgca gtcgatccag caggcgctga aagaggccga
     1441 gatgatggcc gactgcaaga tcacgcgcgt ctacaccggc atcaccggct cgcacatccg
     1501 cggccagaac tcgaccggca tggtgatcgt gcgcgacaag gaagtgacgc cggtcgacgt
     1561 gacgcgcgtc gtcgagaccg ccaaggcgat caacattccg aacgaccagc ggctcttgct
     1621 ggtcgagccg caggagttcg tgatcgacgg ccacgaggtc aaggagccga tcggcatgag
     1681 cggcggccgc ctcgaggtca aggtccacat cgtcaccggc gcgcagagcg cggccgagaa
     1741 catcgtcaag tgcgtgcgcc gatgcggcct cgaggtcgac cagctggtgc tcaacccgag
     1801 cgcctcgagc catgccgtgc tgaccgacga cgagaaggat ctcggcgtcg cgctggtcga
     1861 catcggcgcc ggcaccaccg acgtcgccat cttcaccgac ggatcgatcc gccacaccgc
     1921 ggtgatcccg atcgcgggcg acctgatcac cagcgacatc gcgatggcgc tgcgcacgcc
     1981 gaccaaggac gccgaggaga tcaaggtcga gtacggcgtc gccaagcagc tgctcgccga
     2041 tccgaacgag cagctcgagg tgccgggcct cggcgaccgg ccgccgcgca tgctctcgcg
     2101 ccaggcgctg gccggcgtga tcgagccgcg catcgaggag atctattcgt tggtgcatca
     2161 ggtgatccgc gaaagcggct tcgaggagct gctgtcgtcg ggtgtcgtca tcaccggagg
     2221 cgccgcggtg atgcccggca tggtcgagct cggcgaggac atcttcctca agcccgtcag
     2281 gaagggcacg cccacctatc gcggcgcgct gcacgacatg gtggccaatc cgcgctcggc
     2341 gacggtgatg ggcctgctcg aggaggcgcg gctggcgcga acgcgtggcc agcgcgcggc
     2401 gcagcaggcc ggctcggtgc agaccctgtt cgggcgcgcc agggactggt tc
//
LOCUS       DASRWS010000241         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1810710, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000241 DASRWS010000000
VERSION     DASRWS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810710"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFF72_04435"
     CDS             <1..417
                     /locus_tag="VFF72_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV92439.1"
                     /translation="MVIAAGLGPFAGTLALGLHTTGVLGRLYAESLENVPPEAERALR
                     EAGSGRVAAFAYATLPLLTTQGVAYALYRLEMNIRMAAVLGFVGAGGLGQLLYFHLSI
                     FQQPQAATLLIAMFLLVFAVDSFSAAVRQRLAPAHG"
     gene            complement(465..1706)
                     /locus_tag="VFF72_04440"
     CDS             complement(465..1706)
                     /locus_tag="VFF72_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92440.1"
                     /translation="MSGGKAGAFRSLRVRNYRLWAAGAFVSNIGTWMQRTAQDWLVLT
                     GLTHRSASAVGIVMALQFGPSLLLLPLTGLAADHLDRRKVLLVTQATMGTLAMCLGLL
                     TVFGVVQLWHVYVFALLLGCTTAFDAPARQTFVSELVGTTDLANAVALNSTSFQGARM
                     IGPAAAGLLIGAVGSGWAFLANAGSYAAVLCALAMLRTGELHRTERPPLRRGSLAEGL
                     RYVGGRSDLKVVLAMLFLIATFGLNFAIFIATMAVSVFHVGADQYGLLTSMMAIGSVT
                     GALLSARRAMPRIAVLVGGAAVFGVGCALGAAMPDYWLFGATLVLIGMASQTFMTTAN
                     STIQLACDPPMRGRVMAIYLAIALGSTPIGAPIVGWVADAFGPRWALAVGAAAGLAAA
                     LVGWRYIARIAHEKEAPPAPG"
     gene            complement(1773..2996)
                     /locus_tag="VFF72_04445"
     CDS             complement(1773..2996)
                     /locus_tag="VFF72_04445"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZV92441.1"
                     /translation="MTTTASAASVGLSAERLARIDRFLAERYIESGRLPCALLQVARG
                     GEVVHRSVLGHASLERRQPLAEDTIFRIYSMTKPVTSVAFMMLVEEGKVAIDDPVHRW
                     IPAWRDLAVYRSGLPGAFVTRRVDEPMRIVDLLRHTSGLTYAFQNRTSVDAAYRAGNV
                     DAFEATSLADFVDALGRLPLEFSPGTAWNYSVSTDVLGHLVALVSDMPFDEFVRRRIL
                     GPLGMIDTDFHVTDDKAERFAECYERSPSGQLAKASTHDYRRPPPAPSGGGGLVSTAA
                     DYMRFCEMLRRGGELGGVRLLGPKTLALMRANHLPGRRDLAELSVSMFSESIYQGVGF
                     GLGFAVTTDPARTGLAGSPGEYWWGGMASTAFWIDPVEDVCVVFMTQLIPSSSYPIRR
                     ELRTMVNAAIVDSKA"
     gene            complement(3069..3854)
                     /locus_tag="VFF72_04450"
     CDS             complement(3069..3854)
                     /locus_tag="VFF72_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92442.1"
                     /translation="MRTAVYQRQPAERVHVFDATPMPWEEQARSGLRMKTIRIDDGRG
                     EFLGLIGFDRFVRSAVHQHQGVASSFILEGGLTDYHGPVHQHEMGINFRGSTHDAMSY
                     VPTVLVSKLEGPVTYPKSEQLISGIHAGSTYSDFRNPDPHIPPELNVPVDSLVPVQTG
                     VAGLTRQPIYDYGGSGLVRRILQWKLRPETVVPTWQASDWVELWVRGGELVVNDERAW
                     ANCFVVIEPGATVRMSAPFGAIALVWAEGAESMAASDANLMGF"
BASE COUNT          636 a         1323 c         1397 g          551 t
ORIGIN      
        1 atggtgatcg ctgccggcct cggcccgttc gccggcacgc tcgctctcgg tctgcacacg
       61 accggcgtcc tcggccgcct ctacgccgaa agcctggaga acgtgccgcc cgaggccgag
      121 cgcgcattgc gcgaagccgg cagcggccgc gtcgccgcct tcgcctacgc gacgctgccg
      181 ttgctcacga cccagggcgt ggcttacgcg ctgtaccggc tggagatgaa catccgcatg
      241 gccgcggtgc tcggcttcgt cggtgccggc ggcctcggtc agctgctcta tttccacctc
      301 tcgatcttcc agcagccgca ggccgcgaca ctgctcatcg ccatgttcct gctcgtcttc
      361 gcggtcgact ccttcagtgc cgctgtccgg cagcggctcg cgccggcgca cggctgaatt
      421 gcggggcgac gccgcgtcgc cgccgacacg gcgaggcgcg cacctcagcc cggcgccggc
      481 ggcgcctctt tctcgtgcgc gatccgggcg atgtagcgcc agccgacgag cgccgcggcc
      541 agtcccgccg cggcaccgac ggcaagagcc cagcgcggac cgaaggcatc cgccacccag
      601 ccgacgatgg gcgcgccgat cggcgtgctg ccgagggcga tggcgagata gatggccatg
      661 acacggccgc gcatcggcgg atcgcacgcc agctggatcg tgctgttggc cgtggtcatg
      721 aaggtctgcg acgccatccc gatcaggacc agcgtcgccc cgaacagcca gtagtcgggc
      781 atcgccgccc cgagcgcgca gccgaccccg aacaccgccg cgccgccgac gagcacggcg
      841 atgcgcggca tcgcgcgccg cgccgacagc agcgccccgg tcaccgagcc gatcgccatc
      901 atcgaggtga gcaggccgta ctggtcggcg ccgacatgaa agacgctgac cgccatggtg
      961 gcgatgaaga tcgcgaagtt gaggccgaag gtcgcgatca ggaacagcat cgcgagcacg
     1021 accttcaggt cgcttcgtcc gccgacgtag cgcagcccct cggccaggct gccgcggcga
     1081 agcggcggcc gctcggtccg gtgcagctcg ccggtgcgca gcatcgccag tgcgcaaagc
     1141 accgcggcgt acgaccccgc attggcgaga aaggcccagc ccgagccgac ggcgccgatg
     1201 agcaggcccg ccgcggccgg gccgatcatg cgggcgccct ggaacgaggt cgagttgagc
     1261 gccacggcat tggccaggtc ggtcgtgccg acgagctccg acacgaaggt ctgccgcgcc
     1321 ggtgcgtcga acgccgtcgt gcagccgagg agcagcgcga agacgtagac gtgccagagc
     1381 tggacgacgc cgaacaccgt cagcaggccg agacacatgg cgagcgtgcc catcgtcgcc
     1441 tgcgtcacca gcagcacctt gcgccggtcg agatgatcgg cggcaaggcc ggtcagcggc
     1501 agcagcagca gcgacggccc gaactgcagc gccatgacga tgccgacagc cgaggcgctg
     1561 cgatgggtga ggccggtgag gacgagccag tcctgcgcgg tgcgctgcat ccaggtgccg
     1621 atgttcgaga cgaacgcgcc ggctgcccag agccggtagt tgcgcacgcg cagcgagcga
     1681 aaggcgccgg ccttcccgcc gctcatcggt ggccgggctc gacggtgcgg accagggcag
     1741 gatgcatcgc cagcgtcgcg tccacagggc cgtcaggcct tcgagtcgac gatcgccgcg
     1801 ttcaccatcg tgcgcagctc gcggcggatc ggatacgagc tcgaagggat gagctgcgtc
     1861 atgaagacga cgcacacgtc ctcgaccgga tcgatccaga acgcggtcga ggccatgcct
     1921 ccccaccagt attcgccggg cgagcccgcc aggccggtgc gcgcgggatc ggtcgtcacg
     1981 gcaaagccga ggccgaagcc gacgccctgg tagatcgatt cggagaacat cgacaccgac
     2041 agctcggcga ggtcgcggcg gcccggcagg tggttggcgc gcatcagcgc cagcgtcttc
     2101 ggcccgagca ggcgaacgcc gccgagctcg ccgccgcgtc gcagcatctc gcaaaaacgc
     2161 atgtagtctg cggccgtcga gacgagcccg ccgccgcccg agggtgcagg gggcggcctg
     2221 cggtagtcgt gggtcgaagc tttcgcgagc tgtccggagg gcgatcgttc gtaacactcg
     2281 gcgaagcgtt cggccttgtc gtcggtcacg tgaaagtcgg tgtcgatcat gccgagcggg
     2341 ccgaggatgc ggcgacgcac gaattcgtcg aacggcatgt ccgagacgag ggcgaccagg
     2401 tggccgagca cgtcggtcga gacggagtag ttccaggccg tgccgggcga gaactcgagc
     2461 ggcaggcggc cgagcgcatc gacgaagtcg gcgaggctgg tcgcttcgaa cgcgtcgaca
     2521 tttccggccc gataggcagc gtcgaccgac gtgcggttct ggaaggcgta ggtgaggccg
     2581 gaggtgtgac gcagcagatc gacgatgcgc atcggctcgt cgacccggcg cgtgacgaag
     2641 gcgccgggca ggcccgagcg atacacggcg aggtcgcgcc aggctggaat ccaccggtgc
     2701 accgggtcgt cgatggcgac cttgccttcc tcgacgagca tcatgaaggc gacgctcgtg
     2761 accggcttgg tcatcgagta gatgcgaaag atcgtgtcct cggcgagcgg ctgcctgcgc
     2821 tccagcgagg catggccgag aacgcttcgg tgcacgacct cgccgccgcg ggccacctgc
     2881 agcagcgcgc agggcaggcg gcccgattcg atgtagcgct cggcgaggaa gcggtcgatg
     2941 cgggcgaggc gttcggccga aagtccgacg ctggcggcgg aagcggtggt ggtcatcgcc
     3001 cgattctcca tcgttgcccc gggcgacgtg gcgacgacgg tggctgcgcc tgaagcgccg
     3061 cttcggactc agaatcccat caggttggcg tcgctcgcgg ccatcgattc agctccttcg
     3121 gcccagacca gggcgatcgc tccgaacggc gcgctcatgc gcaccgtcgc accgggctcg
     3181 atgacgacga agcagttggc ccaggccctc tcgtcgttga cgacgagctc gccgccgcgc
     3241 acccacagtt ccacccagtc gctggcctgc catgtcggca cgaccgtctc ggggcgcagc
     3301 ttccactgca ggatccggcg gaccaggccg ctgccgccgt agtcgtagat cggctggcgc
     3361 gtcaggccgg cgacgccggt ctgcaccggc acgagcgaat cgacgggcac gttgagctct
     3421 ggcggaatgt gcgggtcggg attgcggaag tcgctgtacg tcgagccggc gtgaatgcct
     3481 gaaatgagct gctcgctttt cggataggtg accggacctt cgagtttgga gacgagcacg
     3541 gtcggaacgt aggacatcgc gtcgtgcgtc gagccgcgaa agttgatgcc catctcgtgc
     3601 tggtgcacgg ggccgtgata gtcggtgagg ccgccttcga gaatgaagct cgaggcgacg
     3661 ccctggtgct ggtggacggc gctgcgcaca aagcggtcga agccgatcag gccgaggaat
     3721 tcgccgcgcc cgtcgtcgat gcggatcgtc ttcatgcgca gcccggaccg cgcctgctct
     3781 tcccacggca tcggcgtcgc atcgaagacg tggacccgct cggcgggctg gcgctgatag
     3841 acggcggttc gcatgcgggc acctcgcggg gggactgcgg cgactgtacc ccagacgcac
     3901 ggcaatg
//
LOCUS       DASRWS010000242         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2974905, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000242 DASRWS010000000
VERSION     DASRWS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974905"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFF72_04455"
     CDS             complement(<1..559)
                     /locus_tag="VFF72_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZV92443.1"
                     /translation="MSSVAASTEPVPRRLWRRLIGVWLAFWLLLFLLGVQEYLWSGGR
                     QIWRPLVDYGSSALLATLLAAVQIRRSRRFDALLGQPWRWFARMWAWLPLQLVAFVGA
                     MYGLRYLCYSLAGATYRIDLRAEVLAYEAAKFILYYALLGGIQFGQRSYHAWAVERLR
                     TEQQARLAQQAQLAQLTQQLQPHFLF"
     gene            complement(714..2462)
                     /locus_tag="VFF72_04460"
     CDS             complement(714..2462)
                     /locus_tag="VFF72_04460"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZV92444.1"
                     /translation="MKRFAQLFAELDRSTATLDKVAALKRYFARAAPRDAAWAVYFLA
                     GGKPRQVVPSGVLWATACRHAGIEEWLFDACYQAVGDFAETVSHVLPSPTSESDLGLA
                     EWVEARLLPLRGLAPEEQAMRVASYWDELDTPGRFLLNKLIGGGFRVGVSKLLVQRAL
                     AEGAGIDAKLVAQRMMGYTDGRVAPSAERYEQLTATGGPGPLDLGQPYPFFLAHQLDV
                     DAAQLEAKLASPDDWIVEWKYDGIRAQLVKRASQAWIWSRGEELVTERFPEIVAAASS
                     LPNGTVLDGEVVVWKDGRVQPFNLLQQRIGRKTLTRKVLEDAPAGFIAYDLLEVDGRD
                     LRDEPQADRRARLEELLASRPALHLSPVQSLPTWQDYARLREESRSRGVEGFMLKHRL
                     ARYGTGRRKQDDLAAGTWWKWKIDPLSVDCVLVYAQAGHGRRASVYTDYTFAVWNRAP
                     RDAAEAAAVVEAIAKREPAQAEALQLVAFAKAYSGLTDEEFKAVDRVIRQTTLEKFGP
                     VRSVRPSLVFELGFEGIQRSARHKSGIAVRFPRMLRIRDDKPLHEADTMQSLEALLDL
                     QQRGDAPAEPPSDVPS"
     gene            complement(2459..3637)
                     /locus_tag="VFF72_04465"
     CDS             complement(2459..3637)
                     /locus_tag="VFF72_04465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HZV92445.1"
                     /translation="MAAMSDSLPDLVVQRPDGLYCPPGDFYIDPWRPVERAVITHAHA
                     DHARRGHVRYLAAAPAEGVLRSRLGRDIALQTLAYGETIDHFGVRVSLHPAGHVLGSA
                     QVRLEHRGRVWVASGDYYVAGAVAAPGNDNPTCAPFEPVRCHCFITESTFGLPIYRWQ
                     AQQVLFAEIDDWWRGNAAAGRASLLLAYSFGKAQRILAGVDRTIGPIYVHGAVEPLNR
                     AYREAGVDLPETPLVVDVKDRAAFKGALVIAPPSVQGSTFTRRLGDHGDAFASGWMQL
                     RGARRRRSVDRGFVLSDHADWPGLQRAIAATGARRVIVTHGYEAVMVRWLTEQGFEAG
                     AFATEYGSEDDEMPASTEAAAAASEIAADHVAEGVDETTAAAVDVDGADEPPVTDAGA
                     "
     gene            3855..4262
                     /locus_tag="VFF72_04470"
     CDS             3855..4262
                     /locus_tag="VFF72_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV92446.1"
                     /translation="MDALNALFTVATGSTEPIYRQLVEQIRRRVVAGQWAPGQPMPSV
                     RELAEALAVNPMTVSKAFGQLESQGVLERRRGLSMVVAAQAGRATAADRAALLRPTLE
                     QAAGESRQLALSDSRALALFKSVLQETKPTDEE"
     gene            4265..5143
                     /locus_tag="VFF72_04475"
     CDS             4265..5143
                     /locus_tag="VFF72_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92447.1"
                     /translation="MQPSIGSTVTIRNLDLAYSGHSVLKGLDWNLPRGQVVGLLGRNG
                     AGKTSLLEAMLGLRDNATGQINLFDQPVARLDDTARERIGYVPQQSDLFEWLTPAQLL
                     AYFRSFYSHWNEAKVETLLTRWSIDRHLPIGKLSGGQQQRLSIIRAMAHEPDLLILDE
                     PVASLDPAGRRDFLQEIVAQVIDRDTTVVFSTHILSDLERVAFDVAFLSEGKIVLQAQ
                     LDELKDDVRSLAGPTLMVRQLIQEQGWQVLCESPSGSDRTRVLARREPEMEIRSTPGI
                     DVERLTLEDLFLELTR"
     gene            5254..>5672
                     /locus_tag="VFF72_04480"
     CDS             5254..>5672
                     /locus_tag="VFF72_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92448.1"
                     /translation="MVAVAVLIPALVWMTAPAAEHISIAPILAAALLIAGLMGVWTAI
                     VFNVLLQNHPRTARIVPCQLRALRQALWSAAAFASAASAVICGLAGGPVLMTFACAVL
                     ACTVIAACMRWPIFWFAIILIASTTAVWIRSDKINAIA"
BASE COUNT          901 a         1922 c         1970 g          879 t
ORIGIN      
        1 tgaacaggaa gtgcggctgc agctgctgcg tgagctgggc gagctgggcc tgctgggcca
       61 gccgcgcctg ctgctcggtg cgcaggcgct cgaccgccca ggcgtgatac gagcgctggc
      121 cgaactggat cccgcccagc agcgcgtagt agaggatgaa cttggcagct tcgtaggcca
      181 ggacttcggc acgcaggtcg atccggtacg tcgcgccggc gagcgaatag cagagatagc
      241 gcaggccgta catcgcgccg acgaacgcca ccagctgcag cggcagccat gcccacatcc
      301 gcgcgaacca gcgccacggc tggccgagca aggcgtcgaa gcggcgcgag cgccggatct
      361 gcacggccgc cagcagcgtc gccagaagcg cgctcgaccc gtagtcgacc agtggccgcc
      421 agatctgccg gccgccggac cagagatatt cctggacgcc gagcaggaac agcagcagcc
      481 agaaggccag ccagacgccg atgaggcgtc gccagaggcg cctgggaacg ggctcggtcg
      541 aggcggcgac gctgctcatg cgacgcggat gatgcgccaa tgtgcgcgac cgggcagtgg
      601 accgacaagc gatatcggca acgatcggtc atcgagccgc gacttcggaa ccatcatgcc
      661 ggtgccgcga tgcatgcctc gggtcaaggc atgccgacgt cagcggcggt cgatcacgaa
      721 ggcacgtccg aaggtggctc ggccggcgca tcgccgcgtt gctgcaagtc gagcagggcc
      781 tcgagcgact gcatcgtgtc ggcctcgtgc agcggcttgt cgtcgcggat gcgcagcatg
      841 cgcgggaaac gcacggcgat gccgctcttg tgccgcgcgc tgcgctggat gccttcgaag
      901 ccgagttcga agacgaggct cggacggacg ctgcgcaccg ggccgaactt ctcgagcgtc
      961 gtctggcgga tgacccggtc gactgccttg aattcctcgt cggtgagccc cgagtacgcc
     1021 ttcgcgaagg ccaccagctg gagtgcctcc gcctgcgccg gctcgcgctt cgcgatcgct
     1081 tcgacgaccg ccgccgcttc ggccgcgtcg cgcggcgcgc ggttccacac cgcgaacgtg
     1141 tagtcggtat agacgctggc gcgccggcca tgccccgcct gggcgtagac gaggacgcaa
     1201 tcgacgctga gcggatcgat cttccacttc caccacgtgc cggcggcgag atcgtcctgc
     1261 ttgcgccgac cggtgccgta gcgcgccagg cgatgcttga gcatgaagcc ctcgacgccg
     1321 cggctgcgcg attcctcgcg cagcctcgcg tagtcctgcc aggtcggcag cgactgcacc
     1381 ggcgacaggt gcaatgcggg ccgcgacgcg agcagttcct cgagtcgcgc tcgtcgatcg
     1441 gcctgcggct cgtcgcgcag gtcgcggccg tcgacttcga gcaggtcgta ggcgatgaag
     1501 ccggccggcg cgtcctccag gaccttcctc gtcagcgtct tgcggccgat ccgctgctgc
     1561 agcaggttga acggctggac gcggccgtcc ttccagacga cgacctcgcc gtcgagcacg
     1621 gtgccgttgg gcagcgacga cgccgccgcg acgatctccg gaaagcgctc ggtgacgagc
     1681 tcctcgccgc gcgaccagat ccacgcctgc gacgcgcgct tgacgagctg ggcgcggatg
     1741 ccgtcgtact tccactcgac gatccagtcg tcgggcgacg ccagcttcgc ctcgagctgc
     1801 gccgcgtcga catcgagctg atgggcgagg aagaagggat acggctggcc gagatcgagc
     1861 gggccggggc cgcccgtcgc cgtgagctgc tcgtagcgct cggcgctcgg cgcgacgcgg
     1921 ccatcggtgt agcccatcat gcgctgggcc acgagcttgg cgtcgatgcc ggctccctcg
     1981 gccagggcgc gctgcaccag cagcttcgag acgccgaccc gaaagccgcc gccgatcagc
     2041 ttgttgagca ggaatcggcc cggggtgtcg agctcgtccc agtacgaggc gacgcgcatc
     2101 gcctgctctt ccggcgcgag gccgcgcaac ggcagcagac gcgcctcgac ccattcggcg
     2161 aggccgaggt cgctctcgct cgtgggcgac ggcagcacgt gggagacggt ctcggcgaag
     2221 tcgcccaccg cctggtaaca cgcgtcgaag agccactcct cgatgccggc gtggcgacac
     2281 gcggtggccc agagcacgcc gctcggcacg acctggcgcg gcttgccgcc ggccaggaaa
     2341 tagaccgccc acgcggcgtc tcgcggcgcg gcccgggcga agtagcgctt cagcgcggcg
     2401 accttgtcga gcgtcgcggt gctgcggtcg agctcggcga agagctgcgc gaatcgcttc
     2461 atgcgccggc gtcggtgacg gggggctcgt cggcgccgtc gacgtcgacc gctgccgccg
     2521 tcgtttcgtc gacgccttcg gccacatggt cggccgcgat ctccgaggcg gcggccgcgg
     2581 cttcggtcga cgcgggcatc tcgtcgtcct cgctgccgta ttcggtggcg aaggcaccgg
     2641 cctcgaagcc ctgctccgtc agccagcgca ccatcaccgc ttcgtagccg tgggtgacga
     2701 tgacacgccg ggcgccggtg gccgcgatcg cgcgctgcag gcccggccag tcggcgtggt
     2761 cggagagcac gaagccgcga tcgaccgagc ggcggcggcg cgcgccgcgc agctgcatcc
     2821 atccgctcgc gaaggcgtcg ccatgatcgc ctaggcggcg cgtgaacgtc gagccctgga
     2881 ccgaaggcgg ggcgatgacg agagcgccct tgaacgcggc gcggtccttg acgtcgacga
     2941 cgagcggcgt ctcgggcagg tcgacgccgg cctcgcgata ggcgcggttg aggggctcga
     3001 ccgcgccgtg cacgtagatg ggcccgatcg tccggtcgac gccggcgagg atgcgctgcg
     3061 ccttgccgaa gctgtacgcg agcagcaggc tcgcccgccc ggcggcggcg ttgccgcgcc
     3121 accagtcgtc gatctcggcg aagagcacct gctgcgcctg ccagcgatag atcggcaacc
     3181 cgaaggtcga ctcggtgatg aagcagtggc agcgcaccgg ctcgaacggc gcgcaggtcg
     3241 ggttgtcgtt cccgggcgct gcgacggcgc cggccacgta gtaatcgccc gaagcgaccc
     3301 agacccggcc gcggtgctcg agccgcacct gcgccgagcc gaggacatgc ccggcaggat
     3361 gcaggctgac gcgcacgccg aagtggtcga tggtttcgcc gtaggcgagc gtctgcaggg
     3421 cgatgtcgcg gccgagccgc gagcgcagaa cgccctctgc gggcgcggcc gcgagatagc
     3481 ggacgtggcc gcgccgcgca tgatcggcgt gggcgtgggt gatgacggcg cgctcgactg
     3541 gacgccaggg atcgatgtag aaatcgcccg gcgggcagta gaggccgtcg ggccgctgca
     3601 cgacgagatc gggcaaggag tcgctcatcg cggccatcgt agccagcgcg gcgcggcgac
     3661 acgacgtgcg ggtcgcacgg acgcatcgcg aataccgcgg cgttcgcggc agcaacgaac
     3721 cctttccacc cccgtcggcg cggcgagccc gggtgcgtgg cccattcgcc ccggtgatga
     3781 ccacgagacg gacagggcga catcgatcga tcgacttgtg tcttgcgtcc tggtgtacta
     3841 gtatgctaat acacatggac gccctgaacg ctcttttcac cgtggcgacc ggctcgaccg
     3901 aaccgatcta tcggcagttg gtcgagcaga tcaggaggcg ggtggttgcc ggccagtggg
     3961 cgccgggcca gccgatgcca tcggtccgag agcttgccga ggccctggcc gtcaacccga
     4021 tgacggtgtc gaaggcattc ggccagctcg aatcgcaggg tgtcctcgag cgtcgccgtg
     4081 gcctttccat ggtggtcgcc gcgcaagccg ggcgagccac ggcagcagac cgcgcggcct
     4141 tgctgcgacc gacgctggaa caggcggcag gcgagtcgcg tcagctggcg ctgtccgact
     4201 cgcgagcgct ggcgctgttc aagtccgtcc tgcaggaaac gaaacccacc gacgaggagt
     4261 gagcatgcag ccttcaatcg gttcaaccgt cacgatcagg aacctcgatc tggcctatag
     4321 cggccactca gtgctgaaag gcctcgattg gaatctgcct cgcggtcaag tcgtcggcct
     4381 gctcggccga aacggcgccg gaaagacgag cctgctcgag gccatgctcg ggttgcgcga
     4441 caacgcaacc ggccagatca acctgttcga ccaaccggtg gcgaggctcg acgacaccgc
     4501 gcgcgaacgc atcggctacg ttccgcagca aagcgacttg ttcgaatggt tgacaccggc
     4561 ccagctcctg gcctacttca ggagctttta ttcgcattgg aacgaagcca aggtcgaaac
     4621 gctgctcacg cgctggtcga tcgatcgcca tctgccgatc ggcaagctct ccggcggcca
     4681 gcagcagcgc ctctccatca tccgcgccat ggcgcacgag cccgatcttc tgatcctcga
     4741 tgagcctgtc gccagcctgg atccggccgg ccggcgcgac ttcctgcagg agatcgtcgc
     4801 ccaggtcatc gaccgcgaca ccacggtcgt cttttccacc cacatcctct ccgacctcga
     4861 acgggttgcc ttcgacgttg ccttcctgag cgaagggaag atcgtgctgc aggcgcaact
     4921 cgacgagttg aaggatgacg tgcgcagcct cgccgggccg acgttgatgg tgcgacaact
     4981 cattcaggag cagggctggc aggtactttg cgagtcgccg tctggttcag accgaacccg
     5041 cgtcctggcg cggcgggagc cggagatgga aatacgttcg acaccaggga tcgacgtcga
     5101 gcgcctgacc ctcgaggatc tctttctgga gctgacgcga tgatcggcac tccgccttct
     5161 tcgcgccctg gccaactgac ccggacaaga cgggttctcc tgtctgcctg gcaatcaggg
     5221 agcaacccgg gcccgtttcg ggcatgggcg ctcgtggtgg ccgttgccgt gctgatccct
     5281 gcgttggtgt ggatgacggc gcccgcggcc gaacacattt cgattgcacc catcctggct
     5341 gcggctctgc tcatcgccgg cctgatgggg gtctggacgg caatcgtctt caatgtcctg
     5401 cttcagaatc acccgcgaac ggcccggatc gtcccctgcc agttgcgagc gctgcgccag
     5461 gccctgtgga gcgccgccgc tttcgcaagc gccgcgtccg cagtgatctg cggcttggca
     5521 ggtggcccgg tgttgatgac ttttgcctgc gcggtactgg cctgcaccgt gattgcggcg
     5581 tgcatgagat ggccgatttt ctggttcgcg atcatcctga ttgcctcgac gaccgccgtc
     5641 tggatcagaa gcgacaagat caacgcgatc gc
//
LOCUS       DASRWS010000243         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2117399, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000243 DASRWS010000000
VERSION     DASRWS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117399"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFF72_04485"
     CDS             complement(<1..1346)
                     /locus_tag="VFF72_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZV92449.1"
                     /translation="MDSADAPPPITLAELVATLSLVADLGMGRPMERVLRQTVIAMRL
                     AAAAGMEADVCAATYYTSLLTWVGCAADTSDLADLFGDETELYADTHDGDLAGMTMAL
                     FVASHLGRGSSVFRRIGIVGTFLATAGRSVQRVMESHCQSASELARRLDLGADVGEPL
                     LQAFERWDGKGVPGRAGAMQLAPAIRLVHLADNIEAFHASGGPQAAVRVAGERRATQF
                     DPRLVDCFVEHHDEILAGLDETAAWEDVIALDPRLGAPLTADEVDRALGAFADFTDLK
                     SPLRLGHSRGVAARARDAAITLGLPDPDVVMLHRAALVHDVGMIGVPSGVWNETKTWT
                     VSQRERARTHPYLAGRMLARTPLLAMVGRCASLHHERLDGSGYPHGLRGDAIPMPARI
                     LAAADVYCALRQPRPHRPAWDADVAKQTMRDEVRAGRLDGDVVNAVLAAAGHRVRHR"
     gene            complement(1352..1555)
                     /locus_tag="VFF72_04490"
     CDS             complement(1352..1555)
                     /locus_tag="VFF72_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92450.1"
                     /translation="MTVEVRGKAMAAIARLQENIHAVSAAVGSRSVSMNGSVMSLQAR
                     SGPERAAAVDADERSAPCHREEG"
     gene            complement(1604..2920)
                     /locus_tag="VFF72_04495"
     CDS             complement(1604..2920)
                     /locus_tag="VFF72_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZV92451.1"
                     /translation="MAKAIIELESVGKSFRSADGTARPVLEGVDFRLAEGEIVSLLGP
                     SGSGKSTMLRIMAGLIGVDAGDVRYRGQPLYGPARGVSMVFQSFALFPWLTVQGNVEL
                     GLEARGVAPAERARSAEAAIRMIGLAGFEGALPRELSGGMRQRVGIARALVVEPEVLL
                     MDEAFSALDVLTGERLREEILELWSSGEMPTKAILIVSHDIEEAVLMADRVLVLGTHP
                     GRIRFELPVSLPRPRDRDSIEVRGLIDEVYALMTTGEPRAGRPAEEPAVLQLAERLPE
                     ADIGRMDGLLELLAGDTFDGRADLPRLAEETELTDDELLPLAQALSLLGLARITEGDL
                     LLTPLGKRYVDDEHETRQKLFGAQLLAHVPLVAHIRASLDQEPSGELPEEPFVRLLSE
                     SLDAAEAERVLQTAIEWGRHGEVFEYDYNTGLIRLPRSEAAAETAS"
     gene            complement(2953..>3784)
                     /locus_tag="VFF72_04500"
     CDS             complement(2953..>3784)
                     /locus_tag="VFF72_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV92452.1"
                     /translation="VGAGLLGRSFRLFARPYDGTSIRARARPADPVWSRVFDASIAAG
                     ALFAAWHLVSYIHGEVDVAEVAHVFLLALYTLARVLVLIALASLVWVPIGVWIGMNPR
                     WSGRLQAVAQFLAAFPANLTFPVAVLFIVKWRLNPDIWLSPLMVLGTQWYLLFNVIAG
                     ASTIPAELRYAARNLGLGGWLRWRRYLLPAVFPSFVTGAITASGGSWNASIVAEFVTW
                     GDTTLRAQGLGSYIAQMTAVGDFPRIALGIGVMCIFVMAMNHFLWRRLYRLAEDRMHF
                     "
BASE COUNT          636 a         1275 c         1332 g          541 t
ORIGIN      
        1 cgatgtcgca cgcggtggcc ggcggcagcg agaacggcgt tgacgacgtc gccgtcgagc
       61 cggccggcgc gcacctcgtc tcgcatcgtc tgcttcgcga catcggcgtc ccatgccggg
      121 cgatgggggc gaggctggcg cagtgcgcag tagacatcgg cggcggcaag gatccgcgcc
      181 ggcatcggta tcgcgtcgcc gcgaaggccg tgcggatagc cggatccgtc gagcctttca
      241 tggtgcagcg acgcgcagcg gccgaccatc gccagcaggg gcgtgcgagc cagcatgcgt
      301 ccggccaggt acggatgggt acgggctcgt tcgcgttgag agacggtcca cgtcttcgtc
      361 tcgttccaca cgccgctcgg cacgccgatc atgcccacgt cgtgcaccag cgcggcgcga
      421 tgcagcatca ccacgtcggg gtcgggcagg ccgagcgtga tcgccgcgtc cctggcgcgg
      481 gcggcaacgc cgcgcgagtg ccccagacgc agcggcgact tgaggtcggt gaagtcggcg
      541 aaggcgccga gggcccggtc gacctcgtcg gcagtgagtg gggcgccaag acgagggtcg
      601 agggcgatga cgtcctccca cgccgcggtc tcgtcgaggc cggccagtat ctcgtcgtgg
      661 tgctcgacga agcagtcgac cagccgggga tcgaactgcg ttgcgcgtcg ctcgccagcg
      721 acgcgcactg cagcctgcgg accgccggac gcgtggaacg cctcaatgtt gtcggcgagg
      781 tgcacgaggc ggatcgccgg agccagctgc atcgcgccgg cgcggcccgg gacacccttg
      841 ccgtcccatc gttcgaatgc ctggagcagc ggctcaccga cgtcggcgcc aaggtcgagg
      901 cgccgggcga gctcgctggc agactggcag tgcgactcca tcacccgctg cacggagcgc
      961 cccgcggtgg cgaggaacgt gcccacgatg ccaatgcgcc tgaacacgga gctgccccga
     1021 cccaagtggc tggcgacgaa cagcgccatc gtcatgccgg ccaggtcgcc atcgtgggtg
     1081 tcggcataga gctcggtctc gtcgccgaag agatcggcca ggtcgctggt gtcggcggca
     1141 cagccgaccc acgtcaacag cgatgtgtag tacgtcgccg cgcagacgtc cgcctccatg
     1201 ccggcggcgg cggccagccg catcgcaatg acggtctggc gcaacacccg ttccatcggg
     1261 cgtcccatgc cgagatcggc gaccagcgag agcgttgcga ccagttccgc cagggtgatc
     1321 gggggcgggg catcggccga atccatctcc ctcagccttc ctcgcgatgg caaggagcgg
     1381 aacgctcgtc agcgtcgacg gctgccgcgc gttccggccc tgagcgagcc tgaagagaca
     1441 tcacagatcc gttcatgctg acgctccggg atccgacggc ggccgataca gcgtgaatat
     1501 tttcctgcag acgcgcgatc gccgccatcg ccttgccgcg gacttcaaca gtcaccgcgt
     1561 gctcgatgaa cccgcgattg catcgctcgg cgccccgcga gtgtcaggaa gccgtttcgg
     1621 cggcggcctc cgagcgcgga aggcggatca ggccggtgtt gtagtcgtac tcgaacactt
     1681 ctccgtggcg accccattcg atggcggtct gcagcacgcg ttcggcttcc gcggcgtcga
     1741 ggctttcgct gagaagacgc acgaacggct cttcgggcag ctcgccggat ggctcctggt
     1801 cgaggctggc gcggatgtgc gccaccagcg gaacgtgcgc gagcagctgt gcgccgaaga
     1861 gcttctgccg cgtctcgtgt tcgtcgtcga cgtagcgctt gcccagggga gtgagcagga
     1921 ggtcgccttc ggtgatgcgg gccagcccga gcaaagagag cgcctgcgcg agaggaagaa
     1981 gctcgtcgtc ggtgagctcg gtctcctcgg ccaggcgagg cagatcggcg cggccgtcga
     2041 aggtatcgcc ggcgagcagc tcgaggagac cgtccatgcg accgatgtcg gcttcgggaa
     2101 gtcgctcggc cagctgcagt accgccggct cctcggccgg acggccggcg cgaggctcgc
     2161 cggtcgtcat cagcgcgtag acctcgtcga tcaggccgcg aacctcgatg ctgtcgcggt
     2221 cgcggggccg cggcaaagac accggcaact cgaaacggat gcggccggga tgggtgccca
     2281 ggacgagcac ccggtcggcc atcaggacgg cttcctcgat gtcgtgcgag acgatgagga
     2341 tggccttggt cggcatctcg ccgctgctcc acagctcgag gatttcctcg cgaagacgct
     2401 cgccggtgag gacgtccagc gcggagaagg cctcgtccat gagcagcacc tcgggctcga
     2461 cgacgagggc ccgcgcgatt ccgacgcgtt gccgcatgcc gcccgagagc tcgcggggca
     2521 gcgcgccctc gaagcccgcc aggccgatca tgcggatcgc cgcctcggcg ctcctggccc
     2581 gctccgccgg cgccacgccg cgcgcctcga ggccgagctc gacgttgccc tggacggtca
     2641 gccaggggaa gagcgcgaac gactggaaca ccatgctgac gccgcgcgcc ggcccgtaga
     2701 ggggctgccc gcggtagcgg acatcgccgg catcgacgcc gatcaggccg gccatgatgc
     2761 gcagcatcgt cgacttccct gagcccgaag gtccgagcag cgaaacgatc tcgccttcgg
     2821 cgagtcgaaa gtcgacgccc tcgagaaccg gtcgtgcggt gccgtctgcc gagcggaacg
     2881 acttgccgac cgattcgagc tcgatgattg ccttggccat gcgcgctccg tcgttgccct
     2941 tgtcgttcgc catcagaaat gcatccggtc ttcggccaga cgatagagac gtcgccagag
     3001 gaaatgattc atcgccatca cgaagatgca catcacgccg atccccagcg cgatcctcgg
     3061 gaaatcgccc accgccgtca tctgcgcgat gtagctgccg aggccttgcg cgcgaagcgt
     3121 cgtgtcgccc caggtgacga actcggcgac gatgctggcg ttccatgagc cgccgctcgc
     3181 cgtgatcgcg ccggtgacga agctcgggaa caccgccgga agaaggtagc gacgccagcg
     3241 aagccagccg cccaggccga gattgcgggc ggcatatcgc agctctgccg ggatcgtcga
     3301 cgcgccggcg atgacgttga agagcaggta ccactgggtg ccgagaacca tgagcggcga
     3361 cagccagatg tccggattga gccgccactt gacgatgaag agcacggcca ccggaaacgt
     3421 caggttggcc ggaaaggcgg ccaggaactg ggcgacggcc tgcagccttc ccgaccatcg
     3481 cgggttcatg ccgatccata cgccaatcgg tacccagacc aacgaggcga gggcgatgag
     3541 gacgagtacg cgcgccaggg tgtagagcgc cagcaggaag acgtgcgcca cctccgccac
     3601 gtcgacttcg ccatggatgt agctcacaag atgccaggcc gcgaagagag cgccggccgc
     3661 gatgctggcg tcgaagacgc gcgaccagac gggatcggcg ggcctggcgc gggcccggat
     3721 cgacgtgccg tcgtaggggc gcgcgaagag ccggaacgac cggcccagga gccctgcgcc
     3781 gacc
//
LOCUS       DASRWS010000244         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3272679, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000244 DASRWS010000000
VERSION     DASRWS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272679"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VFF72_04505"
     CDS             <1..1316
                     /locus_tag="VFF72_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZV92453.1"
                     /translation="VIGFTNGIAVLIALSQVKDLLGLKIDRWPADFFAQAHTILAHAG
                     GFDPHALALGLACFFGLVLWARLAALPLAESQPDRRGPSTLSGARAMLRRAARAVTRV
                     PGPIVALVTLSALAAWLRLPVETIGTRFGGIPDALPALAWPAFDWSDIQRLFVPTITI
                     AMLGAVESLLCARVADGMTTQRRHDPNQELMAQGIANIVAPFFGGIPATGTIARTVTN
                     LRAGATSPVAGIVHSITLLAIVLVAAPLAASVPLAVLSGVLLFVAWNMGEWHQFSRLR
                     AFAPEHRAKLVATFLLTVMVDLTVAMEVGMALACIVFVYRMGTQFRIDRDPQAQAPSG
                     TLVYRLAGPLFFGAVAKVEALADDLPPATDTIVLDMREVFSIDSSGLAVVENLGRGLA
                     RVGVHLVLRDLSETCRAAFVRSGIELPVVDAAPVQEPGPAHPLRE"
     gene            complement(1427..>2522)
                     /locus_tag="VFF72_04510"
     CDS             complement(1427..>2522)
                     /locus_tag="VFF72_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HZV92454.1"
                     /translation="LPGSTLESTTTSAAPSPLFDRDGWLEIGRLYAVIALLHIAGWGS
                     IVLYARAQPTLVALGVAAYLFGLRHAFDVDHIAAVDDTVRLLLQRGRNALGVGFYFSL
                     GHSTIVLLVAIAVALATTSVSGQLPALHEAGTYIGALVSGAFLLLVGLLNLRVLSGIF
                     RAWSSARTGRHDHTHLESLLLQRGVMNRLFGGRLRRVVDHSWQMYPVGVLFGLGFDTA
                     SEISLLTLTAGASIALPLPAVLALPLLFAAGMLAMDTTDGVLMCKAYRWAFIDPTRKI
                     FYNFATTGLSVAVALFIGSVELAQTAIGMLDLRGQLASFIRNLDFAMLGDAIVGLFIL
                     GWGAAVLAWRFWIRRDSATPVAAGEAMKTS"
BASE COUNT          393 a          875 c          860 g          394 t
ORIGIN      
        1 tcgtcatcgg cttcaccaac ggcattgccg tgctgatcgc gctgtcgcag gtcaaggatc
       61 tgctcggcct gaagatcgac aggtggccgg ccgacttctt cgcacaggcg cacacgatcc
      121 tcgcgcacgc cggcggattc gacccgcacg cgctggcgct cggactggcg tgctttttcg
      181 ggctcgtcct ctgggcccgc ctcgccgcac tgcccttggc ggaaagtcag cccgaccggc
      241 gcggtccttc gaccctttcc ggcgcacggg cgatgctccg tcgcgcggcg cgcgcggtga
      301 cgcgtgttcc cggcccgatc gtcgccctcg tcacgctctc ggccctcgcc gcgtggctgc
      361 gacttcccgt cgagaccatc ggcacgcgct tcggcggcat tcccgacgcg ttgccggcgc
      421 tggcctggcc cgccttcgac tggagcgaca tccagcgact gttcgtgccg acgatcacca
      481 tcgccatgct cggcgccgtc gaatcgctgc tctgcgcccg cgtcgccgac ggcatgacga
      541 cgcagcgacg ccacgacccg aaccaggagt tgatggcgca gggcatcgcc aacatcgtcg
      601 ccccgttctt cggcggcatc ccggcgaccg gcacgatcgc tcgcaccgtc accaacctgc
      661 gcgccggcgc gacgagtccg gtcgcaggca tcgtccattc gatcactctc ctggcgatcg
      721 tcctcgtcgc cgcgccgctc gcggccagcg ttcccctcgc agtgctctcg ggcgtgctcc
      781 tcttcgtcgc ctggaacatg ggcgagtggc accagttctc gcgacttcgc gcctttgcgc
      841 ccgaacaccg ggccaagctg gtcgccacct tcctgctcac cgtgatggtc gatctcacgg
      901 tcgcgatgga ggtcggcatg gcgctcgcct gcatcgtctt cgtctatcgg atgggcaccc
      961 agttccggat cgaccgcgat ccgcaggcac aagcgccgtc cggcacgctc gtctaccggc
     1021 tcgcgggccc gctcttcttc ggcgcggtgg ccaaggtcga ggcactggcc gacgacctgc
     1081 cgccggccac ggacacgatc gttctcgaca tgcgcgaagt tttctcgatc gattcttccg
     1141 gtctggcggt cgtcgagaat ctcggccgcg ggctggcccg cgtcggcgtg cacctcgtgc
     1201 tgcgcgacct gagcgagacg tgccgcgccg ccttcgtgcg atccggcatc gagctgccgg
     1261 tcgtcgatgc cgcgcctgtc caggagcctg gtccggcgca cccgctgcgg gaatgagcgt
     1321 ggcccgatga ccggacgccg tccacgttgc ggcgcggcgg ctccgctgtc aggccgacct
     1381 tctcaaggta tgagtccggt cttgaagccg gatcgtgacg gccttttcag gatgtcttca
     1441 tcgcttcgcc agcggccacc ggagtggccg aatcgcggcg aatccagaac ctccacgcca
     1501 gcacggccgc accccagccg aggatgaaca ggccgacgat ggcgtcgccg agcatcgcga
     1561 aatcgagatt gcggatgaag ctggcgagct gcccgcgcag gtcgagcatg ccgatcgccg
     1621 tctgggcgag ctcgacgctg ccgatgaaga gcgcgacggc gaccgacagg ccggtggtcg
     1681 cgaagttgta gaagatcttg cgcgtcggat cgatgaaggc ccagcggtag gccttgcaca
     1741 tcaggacccc gtcggtcgtg tccatggcca gcatgccggc ggcgaagagc aatggcaggg
     1801 ccaggacggc tggcaggggc agcgcgatcg acgcgcccgc ggtcagcgtc aacaggctga
     1861 tctccgaagc ggtatcgaag cccagtccga acagcacgcc gaccgggtac atctgccagc
     1921 tgtggtcgac gacacgacgc agccggccgc cgaagagccg gttcatgacg ccgcgctgca
     1981 gcagcagcga ttcgagatgc gtgtggtcgt gtctgccggt gcgagccgac gaccaggcgc
     2041 gaaagatccc cgacaggacg cgcaggttga gcagcccgac gaggagcagg aaggcgccgg
     2101 agacgagggc gccgatgtag gtgccggcct cgtgaagcgc cggcagctgt ccactcacgg
     2161 acgtcgtcgc gagggccacc gcgatcgcga cgaggaggac gatcgtcgag tggccgagcg
     2221 agaagtagaa gccgacgccg agcgcattgc gaccgcgctg cagcagcagc cggacggtgt
     2281 cgtcgacagc ggcgatgtgg tcgacgtcga aggcgtggcg cagtccgaac agataggccg
     2341 ccacgcccag ggcgaccagc gtcggctgcg cccttgcgta gaggacgatc gatccccagc
     2401 cggcgatgtg cagcagggcg atgacggcat agaggcggcc gatctcgagc cagccgtcgc
     2461 ggtcgaagag cggagaaggg gcagccgacg tggtcgtcga ctcgagggtc gatccaggca
     2521 gg
//
LOCUS       DASRWS010000245         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2681820, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000245 DASRWS010000000
VERSION     DASRWS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681820"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFF72_04515"
     CDS             <1..1073
                     /locus_tag="VFF72_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZV92455.1"
                     /translation="FHDNDGSICEGVGGFSVADKRKDYGLLLSRGLIRVGLDVPSGAE
                     FIIEHVDDPNHCGPASNDASLYRRPLPATNLRFLSAVMWDGREPDLAHQANDATRGHA
                     QAAQDLSTAQIQQIVDFESSIYTAQARDNGAGNLDAEGAGGGPVRLSKENFFVGINDP
                     VGLNPNGTPFDASAFTIFDSWTKLRSSHDDPMSELRASIGRGQQIFNTRAFTISGVAG
                     LNGQTFSNGVTVPASISGTCTTCHDTPNAGDHSVKAPLNIGISDPDKAPYLPVYTLRN
                     LSTQERVKTTDPGRAMITGKWADVGKFKGPILRALAARAPYFHNGSAATLEQAVDFYD
                     TRFTIGLTASEKADLVAFLRAL"
     gene            complement(1015..>2231)
                     /locus_tag="VFF72_04520"
     CDS             complement(1015..>2231)
                     /locus_tag="VFF72_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV92456.1"
                     /translation="HEERARAERLSEQLRQGQKLESIGRLAGGVAHDFNNLLTVIRGN
                     TDVLARRVKGQAQAEPSIVAIRAATERAAKLTRQLLVFARGGPAEPVPVDLTRKVLDL
                     LGAMSQLVGVGVAIETDFEPGLPAVSVDPLQLEAALLNLAANARDAMDGSGMLYLRLR
                     RSGDWVALQVRDAGPGFDPQVLPRVFDPFFTTKPVGKGTGLGLSQVYGLVKGAGGRVE
                     AANAPGGGAVVTLSFPPAAAAASAPEAEAQPASPPAAGSGAATVLLVDDNEAMLTRAS
                     GALAGQCEVVGSVTDGESVLEAAGILQPDVIVLDISMRGMSGLEVARILRSRDCRAAI
                     VFLTVHEEDEVVLAARAAGALGYVIKARLGTDLAGAVLDASVGRAFVSASTFRAPAGT
                     RRGRPSPTPSGR"
BASE COUNT          353 a          799 c          779 g          300 t
ORIGIN      
        1 tctttcacga caacgacgga tcgatctgcg aaggcgtggg cggcttctcg gtggcggaca
       61 aacgaaagga ctacggcctg ctgctcagcc gcggtctgat tcgcgtcggc ctcgacgtcc
      121 cgtccggcgc cgagttcatc atcgagcacg tcgacgaccc gaaccactgc ggcccggcga
      181 gcaacgacgc gtcgctctac cgccgtccgc tgccggcgac gaacctgcgg ttcctcagcg
      241 cggtgatgtg ggacggccgc gagccggatc tcgcgcatca ggcgaacgac gcgacgcgcg
      301 gccatgcgca ggcggcgcag gatctgtcca ccgcgcagat tcagcagatc gtcgacttcg
      361 agtccagcat ctacacggcg caggcgcgcg acaacggcgc cggcaacctc gacgcggaag
      421 gcgcgggcgg cggacccgtg cggctgtcga aggagaactt cttcgtcggc atcaacgacc
      481 cggtcggcct caatccgaac ggcacgccgt ttgacgcctc cgccttcacg atcttcgact
      541 cgtggacgaa gctccggagc tcgcacgacg atccgatgag tgaactgcgg gcgtccatcg
      601 gccgaggaca gcagatcttc aacaccaggg cgttcaccat cagcggcgtc gccggcctga
      661 acggccagac gttcagcaac ggggtgaccg tgccggcgtc catcagcggc acgtgcacga
      721 cgtgccacga cacgccgaac gccggcgatc actcggtgaa ggcgcccctc aacatcggca
      781 tctccgatcc ggacaaggcg ccgtatcttc cggtgtacac gctgcggaat ctctccacgc
      841 aggaacgcgt gaagacgacc gaccccggcc gcgcgatgat cacgggcaag tgggccgacg
      901 tcggcaagtt caaggggccg atcctgcgcg cgctcgcggc gcgtgcgccg tacttccaca
      961 acggatcggc ggcgacgctc gagcaggccg tcgacttcta cgacacgcga ttcactatcg
     1021 gcctgacggc gtcggagaag gccgacctcg tcgcgttcct gcgggcgctc tgaacgtcga
     1081 tgccgagacg aacgcgcggc cgacgctcgc atccagcacg gcgccggcca gatcggtccc
     1141 gagccgggcc ttgatcacgt agccgagcgc gccggccgca cgggcggcga ggacgacttc
     1201 gtcttcctcg tggacggtga ggaacacgat cgccgcgcgg cagtcgcgcg accggaggat
     1261 gcgcgccacc tccagtcccg acatgccgcg catcgagatg tcgagcacga tcacgtcggg
     1321 ctgcaggatg ccggccgcct cgagcacgga ttcgccgtcg gtcaccgagc cgacgacctc
     1381 gcactgcccg gcgagggccc ccgatgcgcg cgtgagcatc gcctcgttgt cgtcgaccag
     1441 cagcaccgtg gccgcgccgg agccggcggc cggcgggctg gccggttggg cctccgcctc
     1501 gggcgcagat gcggccgccg ccgccggagg gaaggacagg gtcacgaccg cgccgccgcc
     1561 cggggcgttg gccgcctcaa cgcgtccgcc cgcgcccttg accaggccgt agacctgcga
     1621 caggccgagc ccggtgccct tgcccaccgg cttggtggtg aagaaggggt cgaacacccg
     1681 cggcagcacc tgagggtcga agcccggccc tgcgtcgcgc acctgcaggg ccacccagtc
     1741 gccggaccgg cgcagcctga ggtacagcat gcccgagccg tccatggcgt cgcgggcgtt
     1801 ggcggcgagg ttgagcaggg ccgcctccag ctgcagcgga tcgacgctga ccgccggcag
     1861 gccgggctcg aagtcggtct cgatcgccac cccgacgccg accagttgcg acatggcgcc
     1921 gagcaggtcg agcaccttgc gcgtcaggtc caccggcacg ggctcggccg gcccgccgcg
     1981 ggcgaacacc aggagctggc gggtgagctt ggcggcgcgc tcggtggcgg cgcggatcgc
     2041 gacgatggag ggctccgcct gggcctggcc cttcacccgc cgcgcgagga cgtcggtgtt
     2101 gccccggatc accgtgagca ggttgttgaa gtcgtgcgcg acgccgccgg cgaggcggcc
     2161 gatcgactcg agcttctgac cttggcgtag ctgctcggag aggcgctcgg cacgcgcgcg
     2221 ctcctcgtgc a
//
LOCUS       DASRWS010000246         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3546514, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000246 DASRWS010000000
VERSION     DASRWS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3546514"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFF72_04525"
     CDS             complement(<1..426)
                     /locus_tag="VFF72_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZV92457.1"
                     /translation="MPTAAVHVPHSPTPARAFGRYQLRRLLGKSEATMSWLALDSRNQ
                     VETMLTMPRVAPASSAGAAAWLLGARRAARLDHPDLARVVECGIHEHWPYVAVDRRAG
                     VTLDEWLVQHPRPSIDDAAEWIAGVLRGLAFAHDAGVAHL"
     gene            701..1030
                     /gene="trxA"
                     /locus_tag="VFF72_04530"
     CDS             701..1030
                     /gene="trxA"
                     /locus_tag="VFF72_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HZV92458.1"
                     /translation="MSSDLITHTSDASFNADVIEAKTPVLVDYWAEWCGPCKMIAPIL
                     DEVSKTYDGKLRVAKMNVDENRDVPAKYGIRGIPTLMLFKGGQLAATKVGALSKAQLT
                     AFIDSHL"
     gene            1276..>2292
                     /gene="rho"
                     /locus_tag="VFF72_04535"
     CDS             1276..>2292
                     /gene="rho"
                     /locus_tag="VFF72_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZV92459.1"
                     /translation="MHLSELKALHVSELIKMGEALEIENVARMRKQELMFAIMKKRAK
                     GGEQVFGDGVLEVLPDGFGFLRSPEASYMASTDDIYLSPSQVRRFNLHTGDMVEGEVR
                     VPKDGERYFALVKVDKINDLSPEESKHKIMFENLTPLFPKEQFKLERDIKNEENITSR
                     IVDLIAPIGKGQRALLVSAPKSGKTVMMQNLAHALIANYPEVYLIVLLVDERPEEVTE
                     MQRTVRGEVISSTFDEPAARHVQVAEMVIERAKRLVEMKKDVVILLDSITRLARAYNN
                     VLPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTALVETGSRMDEVIYEE
                     FKGTG"
BASE COUNT          447 a          750 c          733 g          362 t
ORIGIN      
        1 gagatgggcg acgccggcgt cgtgggcgaa agccaggccg cgcaggacgc cggcgatcca
       61 ctccgccgcg tcgtcgatcg acggcctcgg atgctggacc agccattcgt cgagcgtgac
      121 gccggcacgc cggtcgacgg ccacgtacgg ccagtgttcg tgaattccgc actcgacgac
      181 gcgggccagg tcaggatggt cgaggcgcgc cgcccggcgg gcaccgagca gccacgccgc
      241 tgctccggcc gaactcgccg gggcgactcg cggcatcgtc agcatcgtct cgacctgatt
      301 gcgcgagtcg agagcgagcc agctcatcgt cgcctcgctc ttgccgagca ggcggcgcag
      361 ctgatagcgg ccgaacgcgc gggccggcgt cggcgagtgt ggaacgtgaa cggcggcggt
      421 cggcatcgcg ggcgggaaaa cgagcaagct cgattggacc gcgaagcagc cgtcaggatc
      481 cgccgatcag gccgccgttc agccggcatc tctgccgcat ttcacgccgc ggcgattcca
      541 aagaagcgga cgcggggcgc gctatcgtct ccataggagg attcggggag ggaccgagcc
      601 gtggcccgaa gtccttgtgt cagaatcatt gcaccctcga cacgggactt gcactcggct
      661 gttgagcctc catctcgggt caggtccaga ggatttcatc atgagcagcg atctcatcac
      721 ccacacgtcc gacgcgtcat tcaacgccga cgtgatcgaa gcaaagaccc cggtcctggt
      781 cgactactgg gccgaatggt gcggcccttg caagatgatc gcgcccatcc tggacgaagt
      841 gtcgaagacc tacgacggca agctgcgcgt cgccaagatg aatgtcgacg agaaccgcga
      901 tgtgccggcg aagtacggca tccggggcat cccgacgctg atgctcttca aaggcggaca
      961 gctggccgcc accaaagtcg gtgcgctgtc gaaggcgcag ttgacggcat tcatcgacag
     1021 tcacctataa tcggttttag caccgggaag gcagccacgc tgccattccg cgaagcagcc
     1081 ggcaatagcc ggatcgtctc cccgacgttc ccatcgccgt tttccggcgt tcatcgacgc
     1141 ggatgccgcg tcgtcacctg acccaactgg catgaacggc cgccgcgaag cggcccgctc
     1201 tcccggccgg tcgcaagcgc gcccggccac gcccgtcgag atcccgtcct cgcgacatgt
     1261 cgtcgcgcac cctccatgca tctctccgaa ctgaaagccc tccacgtctc cgagctcatc
     1321 aagatgggcg aggcgctgga aatcgaaaac gtcgcccgca tgcgcaagca ggagctgatg
     1381 ttcgcgatca tgaagaagcg cgccaagggc ggcgagcagg tcttcggcga cggcgtcctc
     1441 gaggtcctgc ccgatggctt cggcttcctg cgctcgcccg aggcgagcta catggcatcg
     1501 accgacgaca tctacctttc gccgagccag gtgcgtcgct tcaacctgca caccggcgac
     1561 atggtcgagg gcgaggtccg ggttcccaag gacggtgagc gctatttcgc gctggtcaag
     1621 gtcgacaaga tcaacgacct ctcccccgag gagagcaagc acaagatcat gttcgagaac
     1681 ctgacgccgc tatttccgaa ggagcagttc aagctcgagc gcgacatcaa gaacgaagag
     1741 aacatcacca gccgcatcgt cgacctgatc gccccgatcg gcaagggcca gcgtgccctc
     1801 ctcgtctcgg cgcccaagag cggcaagacg gtgatgatgc aaaacctcgc gcatgcgctg
     1861 atcgccaact atcccgaggt ctacctgatc gtgttgctcg tcgatgagcg gcccgaggaa
     1921 gtgaccgaga tgcagcgcac cgttcgcggc gaggtcatca gctcgacgtt cgacgagccg
     1981 gcagctcgcc acgtgcaggt ggccgagatg gtcatcgagc gggccaagcg gctggtcgag
     2041 atgaaaaagg acgtcgtgat cctgctcgac tcgatcaccc ggctggcacg cgcctacaac
     2101 aacgtgctgc cctcgtcggg caaggtgctg acgggtggtg tcgacgccaa tgccctgcaa
     2161 cggcccaagc gctttttcgg tgccgcgcgc aatatcgagg aaggtggctc actcaccatc
     2221 atcggcacgg cgctggtcga aaccggcagc cggatggacg aggtgatcta cgaagagttc
     2281 aagggcacgg ga
//
LOCUS       DASRWS010000247         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3836284, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000247 DASRWS010000000
VERSION     DASRWS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836284"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1584
                     /locus_tag="VFF72_04540"
     CDS             148..1584
                     /locus_tag="VFF72_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92460.1"
                     /translation="MNDDDANGDTTGASGQSDGQAAARGATERGEHDPWLREALRHAP
                     DAALAAPAALREAILAEARSAIRPRSSRRTALPDRAIAFWDWLARPAVAAGFASVMAA
                     TIVGLMWWDRPMDETMPPPERAEPLPAASPGPQLAAPVAIAAKTAMPPEPTPPGAMQN
                     KADDESPRQAHRDAANEARAVPEAKGAPTGPTATSADAQSMPAGQAPAPFPASRAAAE
                     SARGSIRDRATSTLAKKQPGAPIAMPPRQDALDADRPAESARTERTTPHPSDRTAAAS
                     VEAGAAGRVAQDALDGRSAGATSTPPPPAASPAPTVESARDNVAPPAFAAAPVPAAAP
                     TLRRERAAGSGAAGPVETESSTRPSDTPNRPVAALLEAIDRAPQRWRRADSRAAATGL
                     DAATREWLRSVETAAAGRWQEARSDEPQTGSAAGVASLLLERDGQATASLRVEADGVR
                     LVMPSGTRFAPLPVDALARLRETLPPPR"
     gene            1667..>3409
                     /locus_tag="VFF72_04545"
     CDS             1667..>3409
                     /locus_tag="VFF72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZV92461.1"
                     /translation="MPSAESVSDAAAAPLATTSVGSETIRVRGARTHNLKNIDLDIPR
                     NRLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQRMDKPDVDIIEGLSP
                     AISIEQKATSHNPRSTVGTVTEIHDYLRLLFARAGTPYCPTHGLALQAQSVSQMVDAT
                     LALPAQARLMIVAPVARDRKGEFAELFQDMQSQGFVRFRVDGRTVEAADVPKLKKSEK
                     HDIDVVIDRVRVQPGIEQRLAESFEAALRIADGRALVVELDAAPSAGGQHTGASPHEH
                     LFSSRFACPLCDYSLPELEPRLFSFNSPVGACPTCGGLGMTTVFDAERVVAFPTLSLA
                     SGAVKGWDKRNAYTFSLLESVARHYAFDIDQPFESLPERARQILLRGSGSDEIEFVYA
                     AEGARGKQRQVKRRHPFEGILPNLERRFRETDSAAVREDLMRYQSAKPCPDCHGARLR
                     VEARNVFLADAKSGEKEPIFRIEHFTLRDCLDWFRRLRLEGAKAEIADKVIREICARL
                     KFLNDVGLNYLSLDRNAETLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNA
                     RLIGTLRHLRDLGNS"
BASE COUNT          529 a         1282 c         1156 g          442 t
ORIGIN      
        1 tcacgacgac ggcctcggcc tcgacgaggt ggcacgtgcc ctcgaggtcg gcttcgagac
       61 cgcgaagacg cgcctgcgct acgccatgag caagctgcgc acctgcatgg gcgcctatct
      121 cgagccgctg caaccggaaa cgcgcgcatg aacgacgacg acgcgaacgg cgacacgacc
      181 ggagcgagcg gccagtccga tgggcaggcc gcagcgcgcg gcgccacgga acgcggcgag
      241 cacgatccct ggttgcgcga ggcactgcgc catgcccccg acgccgccct cgcagccccc
      301 gccgcgctgc gcgaggcgat cctggctgag gcgcgctcgg cgatccggcc tcgatcctcg
      361 cgacgaacag cgcttcccga ccgggcgatc gcgttctggg actggctggc gcgcccggcc
      421 gtggcggcgg ggttcgccag cgtgatggcg gcgacgatcg tcggcctcat gtggtgggat
      481 cggccgatgg acgaaacgat gccgccgccg gagcgcgccg agccgctgcc cgctgcgagc
      541 ccgggcccgc aactcgccgc accagtcgcc atcgcggcca agacagccat gccgcccgag
      601 ccgacaccgc cgggtgcgat gcagaacaag gccgacgacg aatcgccccg ccaagcacat
      661 cgcgatgcag cgaacgaggc gcgcgcggtc ccagaagcca agggtgcgcc gacgggcccc
      721 acggccactt ccgccgatgc gcaatcgatg cccgccggcc aggcccctgc accctttccg
      781 gcgtcacgcg cggcggcgga gtcggcgcgc gggtcgatcc gcgaccgcgc gacatcgacg
      841 ctcgcgaaga agcagccggg cgcccccatc gcaatgccgc cgcggcaaga cgcgctcgac
      901 gccgatcgtc cggcggagtc ggcgcgcacg gagaggacga cgccgcatcc gagtgaccgt
      961 accgccgccg cttcggtcga ggccggggca gccggaaggg tggcgcagga tgcgctcgac
     1021 ggccgttcgg cgggcgcaac gtcgacgccg ccacctcccg ccgcttcgcc tgcacccacg
     1081 gtcgagtccg cacgagacaa cgtcgccccg cccgcgttcg ccgcggcgcc agtgcccgcg
     1141 gccgcaccga cgttgcgccg cgagcgcgct gcgggatcgg gcgcggccgg acccgtcgag
     1201 accgaatcct cgacccgtcc atccgacacg ccgaaccggc cggtcgcggc gctgctcgag
     1261 gcgatcgacc gggcgccgca acgctggcgg cgcgccgatt cccgggctgc ggcgacgggc
     1321 ctcgatgccg cgacacgcga gtggctccgc agcgtcgaga ccgctgccgc cggacgatgg
     1381 caggaggcgc gcagcgacga gccgcaaacc gggtcggccg caggcgttgc atccctgctt
     1441 ctcgagcgcg acggccaggc cacggcgtcg ctgcgggtcg aagcggacgg ggtgcgcctg
     1501 gtgatgccgt cgggcacccg cttcgctccg ctgccggtcg acgcgctggc gcgacttcgc
     1561 gagaccttgc caccgccgcg ctgatcggtg ccgcccggcc acccgtccgc tggcgccgga
     1621 aaagggtgcg cccgtatgat gatcggttcg ccttttgtcg ccgccgatgc cttctgccga
     1681 gtccgtttcc gacgccgctg ccgcgccact cgccaccacc tccgtcggca gcgagacgat
     1741 ccgggtccgc ggcgcccgca cccacaacct gaagaacatc gatctcgaca tcccgaggaa
     1801 ccgcctggtc gtcatcacgg gtctgtcggg ctcgggcaag tcgagcctcg ctttcgacac
     1861 cctctacgcc gaaggccagc gtcgctacgt cgagagcctc tcggcctatg cgcgtcagtt
     1921 cctgcagcgc atggacaagc ccgacgtcga catcatcgaa ggcctgtcgc cggcgatctc
     1981 gatcgagcag aaggcgactt cgcacaaccc gcgctcgacg gtcggcaccg tcaccgagat
     2041 ccacgactac ctgcgcctgc tcttcgcccg tgccggcacg ccgtactgcc cgacgcacgg
     2101 cctggcgctg caggcgcaaa gcgtcagcca gatggtcgat gcgacgctgg cgctgcccgc
     2161 gcaggcgcgc ctcatgatcg tggcgccggt ggcccgcgat cgcaagggcg agttcgccga
     2221 gctgttccaa gacatgcagt cgcaaggctt cgtccgcttc cgcgtcgacg ggcggacggt
     2281 cgaggccgcc gacgtgccga agctgaagaa atcggaaaag cacgacatcg acgtcgtcat
     2341 cgatcgcgtc agggtgcagc cgggaatcga gcagcgcctc gccgagagct tcgaggcggc
     2401 gttgcggatc gccgacgggc gcgcgctggt ggtggagctc gacgccgcac cgtcggccgg
     2461 cggccagcac accggcgcca gcccgcacga gcacctcttc tccagccgct tcgcctgccc
     2521 gctgtgcgac tactcgctgc ccgagctcga accacgactg ttttcgttca actccccggt
     2581 cggcgcctgc ccgacctgcg gcggactcgg catgacgacc gtgttcgacg ccgagcgcgt
     2641 cgtcgccttt ccgacgctga gcctggccag cggcgcggtc aaaggctggg acaagcgcaa
     2701 cgcctacacc ttctcgcttc tcgaaagcgt tgcccgccac tacgcgttcg acatcgacca
     2761 gccgttcgag agcctgcccg agcgggcgcg ccagatcctg cttcgcggct cgggcagcga
     2821 cgagatcgag ttcgtctacg ccgcggaagg ggcgcgcggc aagcagcggc aggtcaagcg
     2881 caggcatccc ttcgaaggca tcctgcccaa cctcgagcgg cgctttcgcg agaccgactc
     2941 ggccgccgtg cgcgaagacc tgatgcgcta tcagagcgcc aagccctgcc ccgactgcca
     3001 tggcgcgcgg ctgcgcgtcg aggcgcgcaa cgtctttctc gccgacgcga agagcggcga
     3061 gaaggagccg atcttccgga tcgagcactt cacgcttcgc gactgcctcg actggttccg
     3121 gcggctgcgc ctcgaagggg cgaaggcgga gatcgccgac aaggtgatcc gggagatctg
     3181 cgcccgcctg aagttcctca acgacgtcgg cctcaactac ctgagcctgg accgcaacgc
     3241 cgagacgctc tcgggcggcg aggcgcagcg gatccgcctc gcctcgcaga tcggctccgg
     3301 gctgaccggc gtgatgtacg tgctcgacga gccgtcgatc ggcctgcacc agcgcgacaa
     3361 cgcacggctc atcggcacgc tccgccacct gcgcgacctc ggcaacagc
//
LOCUS       DASRWS010000248         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2119896, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000248 DASRWS010000000
VERSION     DASRWS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119896"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1921)
                     /locus_tag="VFF72_04550"
     CDS             complement(77..1921)
                     /locus_tag="VFF72_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV92462.1"
                     /translation="MTKSQARGGAATRPAADESWFGDFGQAGDTQSLIEDSRFTATWQ
                     HEAEARLRRGRAAGVASTTFERIYRVFIAARAALGAALVATLGVANALGLRPGAIVGS
                     LSVAYATLAISMWLLPRLRRGAAPHTFARLRSPQWIATIGADLAFFTALHVLSPGSSF
                     NYAALLVLPVLMAGVLTPRVMALGTAALATLALLGAAWLGLLSGGDVALLMTQAGLVG
                     SGFFVISVLAGELAGRIAREELSARGSMEMARQQAQLNRLVLEEMEDGVLVVDRGGKV
                     RAANPAARRLLAPEGSSRAAPFQLRGVPAWGGLVQAVERAFVEAAWPEAGRDVTLPFA
                     PDLHRTLRARIRFTRKREPGASEDVCVVFLEDLRNVQARNRQEQLAAMGRVSAGIAHE
                     IRNPLAAIAQANALLSEDATDPAQRQLMHMVTENVERLKRIVDDVMELAPGRAQDAAS
                     VDATQHVANICSEWALAAGVPLGERGMLRVDLPAQPVGIVFDAEHLRRVLVNLLDNGH
                     RHASGKPAALLVSLRAEGDAHAEFRVASDGAPIPADVEPYLFEPFFSTRSRGAGLGLY
                     ICRELCERYGARIDYRLLPPDAPRRNEFVVGVRRAALQSSEARLHLTP"
     gene            complement(2010..2249)
                     /locus_tag="VFF72_04555"
     CDS             complement(2010..2249)
                     /locus_tag="VFF72_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HZV92463.1"
                     /translation="MKIVVFLIAVFVLLWLLRGGTGRRAPPAPGPGPRPAQPMLACAQ
                     CGIHLPRDEALPGRGGVFCSEAHRAAYEKAHPGAA"
     gene            complement(2246..3091)
                     /gene="ccsA"
                     /locus_tag="VFF72_04560"
     CDS             complement(2246..3091)
                     /gene="ccsA"
                     /locus_tag="VFF72_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZV92464.1"
                     /translation="MMILSFGPDPGRLSPLAVALSVPSVAALACYVAAAMPADRYASV
                     LRKALLVGWIAQGVSIVIDLVGVGSDVPGARFGFAPALSITIWFVLAVYELESRFVPL
                     PGARRALAWCGIVVVVLAWVYPGQVHPLASSVWAPLHWLLGIASYGLFGVAVLHAWLL
                     NRAERQMRATTAVSRAVNEGLPILRLERLTFRFVAAGFVALSAAIVLGAWFANPWRWD
                     HKTVFSILGWIVFAGLLAGRRAFGWRGPMAARWLYAGAVLLLLAYVGSRFVLEVLLHR
                     APPST"
     gene            3137..4507
                     /gene="ffh"
                     /locus_tag="VFF72_04565"
     CDS             3137..4507
                     /gene="ffh"
                     /locus_tag="VFF72_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZV92465.1"
                     /translation="MASALTDRLSRLVKTMRGQARITEDNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKTRALGAEVVGSLSPGQAFVGIVNRELAATMGEGVAELDLATQPPAV
                     ILMAGLQGAGKTTTTAKLARHLVEKRRKKVLTVSADVYRPAAIEQLKTVTRQAGAEWF
                     PSTANDSPLAIARSALDHARRHYFDVLIVDTAGRLAIDEKLMAEIRELHAELEPVETL
                     FVVDAMQGQDAVNTAKAFKEALPLTGIVLTKMDGDARGGAALSVKAITGAPIKFAGIS
                     EKIDGLEVFDAERHAGRVLGMGDIVGLVEEVQKNVDVAAAQKLADKVKAGDTFDLNDF
                     LSQISQMKKMGGLGGLLDKLPSELTGKAAHLGQADMDRAERDVRRMEGIICSMTPLER
                     RKPELLKATRKRRIASGAGVQVQEVNRLLNQYEQMRDVMKKMKGGGMMKMMKRMGAMK
                     GLGGRR"
     gene            4544..5356
                     /locus_tag="VFF72_04570"
     CDS             4544..5356
                     /locus_tag="VFF72_04570"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HZV92466.1"
                     /translation="MDPYYLISIVAAAAALGLAWWLVRRQGTVDRPEQPADRLDTLAA
                     WPPEPTRILRSQERLAFSTLKLALPGYMILAQVPIARFISVPKRNSYAEWMRRLGSQC
                     VDFVICDVTSQVVAVVEIRPPAEQIGDKLRVRLDRLARTLDAAGIPVHVWNEERMPTI
                     EAARSKILPNAPAVPPGLNRRSAAAPVQVLETADAGFVPAAATAREGVDVIEDGAPES
                     AVTVEGPAVDPFADTDRDWTQGEIIEVKEPAGSTWFDELEESVSPVLPAQRR"
     gene            complement(5373..>6247)
                     /gene="ftsY"
                     /locus_tag="VFF72_04575"
     CDS             complement(5373..>6247)
                     /gene="ftsY"
                     /locus_tag="VFF72_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZV92467.1"
                     /translation="AMYEDLEAALLQADTGSAAAKYLLDDLQARVTRADIRDPASVRR
                     LLAECVAELLAPLEKSLVIGEHVPTVIMVVGVNGAGKTTSVGKLARYLMDHDQKVLLA
                     AADTFRAAAREQLLAWAERADPGLRSDVSTPMGAAAHRIEIVSQEGGDPAGLTFDAVV
                     AGKARGCDVVIADTAGRLATQAHLMEELKKIRRTIGKAQAGAPHEVLLVIDGNTGQNA
                     LAQVQAFDAALQLTGLVITKLDGTAKGGVLAAIALWSRSRPSPLPVYFIGIGEKLADL
                     QTFRAGEFAEALVR"
BASE COUNT         1113 a         2133 c         2145 g          856 t
ORIGIN      
        1 agcagtgtca gttcgtacag ggttcgcaga tcgggttcgt cgtcgacgac gagcaggctg
       61 aagtgcgatg cggtggtcat ggggtgaggt gcaagcgggc ttccgaggat tgcaaggcgg
      121 cgcggcgcac gccgacgacg aattcgttgc gccgcggagc atccggaggc agcagtcggt
      181 agtcgatgcg tgccccgtat ctctcgcaga gctcgcgaca aatgtaaagg ccgagaccgg
      241 caccgcgact gcgggtcgag aaaaatggct cgaacaaata tggctcgacg tccgccggaa
      301 tgggcgcgcc gtcggaggcg acccggaact cggcgtgcgc gtcgccttcg gcacgcagcg
      361 acacgagaag cgctgccggc ttgccgctgg catggcggtg gccgttgtcg agcaggttga
      421 cgaggacccg gcgcagatgt tcggcgtcga agacgatgcc gaccggctgc gccggaagat
      481 cgacccgcag catgccgcgc tcgccgaggg gcacccctgc ggccagcgcc cattcgctgc
      541 agatgttggc gacatgctgc gtcgcgtcga cgcttgcggc gtcctgcgcg cggccgggag
      601 ccagttccat cacgtcgtcg acgatgcgct tcaggcgctc gacattttcc gtgaccatgt
      661 gcatcaactg acgctgcgcc gggtcggtgg cgtcttcgga caacaacgca ttggcctgcg
      721 cgatcgcggc gagcggattg cggatctcgt gcgcgatgcc tgccgatacg cgtcccatcg
      781 ccgcgagctg ctcctgccgg ttgcgggcct ggacgttgcg caggtcctcg agaaagacga
      841 cgcagacgtc ctcgctggcg ccgggctcgc gctttctcgt gaagcggatg cgcgcacgca
      901 gcgttcggtg caggtcgggc gcgaagggca gcgtcacgtc ccggccggcc tcgggccacg
      961 ctgcttcgac aaaggcacgc tcgaccgcct ggacgagccc tccccacgcc ggcacgccgc
     1021 gcaactggaa aggcgctgcg cgactcgacc cttcgggagc aagaaggcgc cgtgccgccg
     1081 gattggcggc gcgcaccttg ccgccgcggt cgacgacgag cacgccatcc tccatttcct
     1141 cgagcaccag gcggttcagc tgcgcctgct gtcgcgccat ctccatgctg ccgcgagcgc
     1201 tgagctcctc gcgcgcgatc cggcccgcga gttcgccggc cagcacgctg atgacgaaaa
     1261 agccgctgcc gaccagtccg gcctgcgtca tcagcagcgc cacgtcgccg cccgagagga
     1321 gaccgagcca ggccgcaccg agcagcgcca gcgtcgccag cgcggccgtt ccgagcgcca
     1381 tcacccgcgg cgtcaggaca ccggccatca gcaccggcag gacgagcagc gcggcgtagt
     1441 tgaagctcga acccggcgac aggacgtgca gcgccgtgaa gaaggccagg tcggcgccga
     1501 tggtggcgat ccattgcggg ctgcgcagcc tcgcgaaagt gtgcggcgcg gcaccacggc
     1561 gcagccgcgg cagcagccac atcgagatcg ccagcgtcgc gtacgcgacg ctcagcgagc
     1621 ccacgatggc gccgggtcga agtccgaggg cgttggccac gccgagggtc gccacaagcg
     1681 ccgcacccag ggccgcgcgg gccgcgatga aaacgcgata gatgcgctcg aaggtcgtcg
     1741 aggcgacgcc cgcggcgcgg ccgcggcgca gtcgcgcttc ggcttcgtgt tgccacgtcg
     1801 cggtgaagcg cgagtcctcg atcagggatt gggtatcgcc ggcctggccg aaatcgccga
     1861 accacgactc gtcggccgcg ggccgcgtcg ccgcgccgcc gcgagcctgt gacttcgtca
     1921 cgcgaggcgc cggcgtcacg tcatcccggt cgcgagtcga gcgcacgagc cgccgtcagg
     1981 aaaacggcac ggcatgggaa gcagccgtct catgcagcgc ccggatgcgc cttctcgtac
     2041 gcggcgcgat gcgcctcgct gcagaagacg ccgccccggc ccggaagcgc ttcgtcacgc
     2101 ggcagatgaa ttccgcattg cgcacaggcg agcatgggct gggccggacg cgggccgggg
     2161 cccggtgccg gcggtgccct gcgtccggta ccgccgcgca gcagccacag caacacgaag
     2221 acagcgatca ggaagacgac gatcttcatg tggacggcgg tgcgcgatgc agcagcacct
     2281 cgagaacgaa acgcgatccg acatacgcaa ggaggagcaa cacggctccg gcgtacagcc
     2341 agcgcgctgc catcggcccg cgccagccga acgcgcgtcg gccggcgagc aggccggcga
     2401 agacgatcca gccgaggatc gagaacaccg tcttgtgatc ccagcgccag ggattggcga
     2461 accaggcgcc gaggacaatg gccgccgaaa gggccacgaa acccgcggcg acgaagcgaa
     2521 acgtcagccg ctcgaggcga aggatcggca gcccttcgtt gacggcacgc gacaccgccg
     2581 tcgtcgcacg catctgtcgt tcggcgcgat tgagcagcca ggcatgcagg accgcgacgc
     2641 cgaacaagcc gtaggaggcg atgcccagca accagtgcaa cggcgcccac accgacgagg
     2701 cgaggggatg cacctggccc ggatagaccc acgcaagcac gacgacgacg atcccgcacc
     2761 aggccagcgc acgacgcgcg cccggcaggg gcacgaagcg gctctccagc tcgtagaccg
     2821 cgagcacgaa ccagatggtg atcgaaagcg ccggggcgaa gccgaagcgc gcccccggaa
     2881 cgtcggaacc gacacccacc agatcgatga cgatcgacac gccttgggcg atccatccaa
     2941 ccagcaaggc cttgcgaagg acactcgcgt atcggtcggc aggcatcgcc gccgccacgt
     3001 agcatgcgag cgccgcgacg ctcggcacgc tgagcgccac agcgaggggc gagagcctgc
     3061 ccgggtcggg accgaacgat agaatcatca tccaagtgta ctttcgcacc cggccgcacg
     3121 gccccgtgcg cgcctgatgg catctgccct caccgaccgc ctgagcaggc tcgtcaagac
     3181 gatgcgcggc caggcccgca tcaccgagga caacgtgcag gacatgctgc gcgaggtccg
     3241 gatggccctc ctcgaagccg acgtcgcgtt gccggtggtg cgcgacttca tcgcccgggt
     3301 gaagactcgc gcgctcggcg ccgaagtcgt cggttcgctc tcgccgggac aggcattcgt
     3361 gggcatcgtc aaccgcgagc tcgccgcgac gatgggcgaa ggcgtcgccg agctcgacct
     3421 ggcaacgcag ccaccggccg tgatcctgat ggccggcctt caaggcgccg gcaagacaac
     3481 gacgaccgcc aagctcgccc ggcacctcgt cgaaaagcgt cgcaagaagg tgctcaccgt
     3541 gtctgccgac gtctatcgcc cggccgcgat cgagcagttg aagacggtga cccgccaggc
     3601 aggcgccgag tggttcccgt cgaccgcgaa cgattcgccg ctggcgatcg cccgaagcgc
     3661 cctcgatcac gcccggcgcc attacttcga tgtcctcatc gtcgacacgg ccggacggct
     3721 ggcgatcgac gagaagctca tggcggagat ccgcgagctg cacgccgagc tcgagccggt
     3781 ggagacgctc ttcgtcgtcg acgcgatgca gggccaggac gccgtcaaca ccgcaaaggc
     3841 gttcaaggaa gcgcttccgc tgaccggcat cgtcctcacc aagatggacg gcgacgcccg
     3901 cggcggagcg gcgttgtcgg tgaaggcgat caccggcgcg cccatcaagt tcgccggcat
     3961 cagcgagaag atcgacggcc tggaggtgtt cgatgccgag cgacacgcgg gccgcgtcct
     4021 cggcatgggc gacatcgtcg gcctggtcga ggaggtgcag aagaacgtcg atgtcgccgc
     4081 agcgcagaaa ctcgccgaca aggtcaaggc cggagacacc ttcgacctga acgacttcct
     4141 gtcacagatt tcacagatga agaagatggg cggcctcggt ggcctcctgg acaagttacc
     4201 ctccgagctc accggaaagg cagcccatct cggccaggcc gacatggacc gggcggagcg
     4261 cgacgtgcgg cgcatggaag gcatcatctg ttcgatgacc ccgctcgaaa gacgcaagcc
     4321 cgagctgctg aaggcgacgc gaaagcggcg catcgccagc ggtgccggcg ttcaggtgca
     4381 ggaagtcaat cgtctgctca accagtatga gcagatgcgc gatgtgatga agaagatgaa
     4441 gggcggcggc atgatgaaga tgatgaagcg catgggcgcg atgaagggcc tcggcggccg
     4501 gcgctgagcg cgtcgagctg ccgcttccac agaccccgcc accatggacc cgtattacct
     4561 catctcgatc gtcgctgcgg ctgcggcgct tggattggcc tggtggctgg tccggcgcca
     4621 gggaacggtc gaccgacccg aacagccggc cgaccgcctc gacaccctgg cggcgtggcc
     4681 acccgagccg acgcggatcc tgcgttccca ggagcggttg gccttcagca ccctcaagct
     4741 cgcgctgccc ggctacatga tcctcgcgca ggtgccgatt gccaggttca tcagcgtgcc
     4801 caagcgcaac tcctacgccg aatggatgcg ccgcctcggc agccagtgcg tcgacttcgt
     4861 gatctgcgac gtcacctcgc aggtcgtcgc ggtcgtcgag atccggccgc ccgccgagca
     4921 gatcggcgac aagctgcgcg tccgcctcga tcgcctggcg cgaacgctcg acgcggcggg
     4981 catcccggtg catgtgtgga acgaggaacg gatgccaacg atcgaggcag cccgcagcaa
     5041 gatcctgccg aatgcgccgg ccgtgccgcc gggactgaat cgcaggtcgg cggccgcgcc
     5101 cgtccaggtg ctggaaaccg cagatgccgg attcgtcccc gccgccgcca cggcccgcga
     5161 aggcgtcgat gtcatcgagg acggcgcgcc cgaaagcgcg gtgaccgtcg aagggccggc
     5221 ggtggatccg ttcgccgaca ccgaccgcga ctggacgcag ggcgagatca tcgaggtcaa
     5281 ggagccggcc ggctcgacct ggttcgacga gctcgaggaa tcggtctcgc cggttcttcc
     5341 ggcgcagcgg cgataggctg gatcggaaag cctcagcgaa ccagcgcctc ggcaaattcg
     5401 ccggcacgaa acgtctgcag gtcggcaagc ttttcgccga tgccgatgaa atacaccggc
     5461 agcgggctcg gccgtgatcg agaccacaac gcaatcgccg ccagaacgcc gcccttggcg
     5521 gtgccgtcga gcttggtgat gacgagcccg gtgagctgca aggcggcgtc gaaagcctgc
     5581 acctgcgcca gggcgttctg cccggtgttg ccgtcgatca cgaggagcac ctcgtgcggc
     5641 gcgccggcct gcgccttgcc gatggtgcgg cggatcttct tcagctcctc catcaggtgc
     5701 gcctgggtcg ccagacggcc ggcggtgtcg gcgatgacga catcgcagcc gcgcgccttg
     5761 ccggcgacga cggcgtcgaa agtgagtccg gccgggtcgc caccttcctg gctcacgatc
     5821 tcgatgcgat gcgcggctgc ccccatcggt gtcgacacgt ccgagcgcaa tccgggatcg
     5881 gcccgttccg cccaggcgag cagttgttcg cgcgcggcgg cgcgaaaggt atcggcagcg
     5941 gcgagcagca ccttctgatc gtgatccatc agatagcgtg ccagcttgcc gacgctggtc
     6001 gtcttgccgg ctccgttgac gccgacgacc atgatcactg tcggcacgtg ctcgccgatc
     6061 acgaggctct tctcgagcgg cgcaagcagc tcggcgacgc attcggcaag caggcgccgc
     6121 acgctcgcag gatcgcggat atcggcccgg gtcacgcggg cctgaaggtc gtcaagaaga
     6181 tatttcgcgg ccgccgagcc ggtgtccgcc tgcagcaatg cggcttcgag gtcctcgtac
     6241 atcgccg
//
LOCUS       DASRWS010000249         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_966835, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000249 DASRWS010000000
VERSION     DASRWS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966835"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFF72_04580"
     CDS             <1..532
                     /locus_tag="VFF72_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92468.1"
                     /translation="GSIKLDGEEISTLPVHRVVRRGLALVPEGRMIVAPLTVEENLRL
                     SGYAGRGVPDALMARVYDLFPRLKERRGQVAGLMSGGEQQMLAIGRALMTEPRMLVLD
                     EPSMGLAPAIVDLVFQAIVTLHRQGQSILLVEQNAEIALSVCDYAYLMKRGSIVMQGT
                     VDALRADASVLEAYLA"
     gene            complement(657..1688)
                     /locus_tag="VFF72_04585"
     CDS             complement(657..1688)
                     /locus_tag="VFF72_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HZV92469.1"
                     /translation="MSEADSSLPALPVLPDSFTALRCRSDGRTAAVGALESMTAAELS
                     PGELLVRTRFAGVNYKDCLAITARARIIEAYPRIPGIELVGDVAASEVASFAPGTPVM
                     AHGFRTGIAFDGGFSPWVRVPAAHAMAVPAGLDVREIAILGVPGFTVGLCLDRFEQLG
                     VTPASGPVAVDGATGAVGMLAIAILARAGFEVVALTGKPSQESTLRALGASQVLETGP
                     LLASRRALETERFAAAIDNVGGALLGWLLKSMRSGGALASVGNASGNDYPGNVLPFIM
                     RSVQVFGVVANAPWPVRHRVWARLASEWRPDFECLEPHVRTIALDALPAHCGRQLAGA
                     TSGRTLVAF"
BASE COUNT          315 a          690 c          720 g          276 t
ORIGIN      
        1 tggctcgatc aagctcgatg gcgaagagat ctccacgctg cctgtccatc gcgtcgtgcg
       61 ccggggtctg gccctggtgc cggaaggccg catgattgtt gccccgctca ctgtcgagga
      121 aaacctcagg ctcagcggct acgccgggcg cggtgtcccg gatgcgctga tggcgcgtgt
      181 ctacgacctg tttccgcgtc tcaaggagcg gcgtgggcag gtcgcgggcc tgatgagcgg
      241 cggcgagcag cagatgctcg cgatcggccg cgccctgatg accgagccgc gcatgctggt
      301 gctcgacgag ccctcgatgg gcctggcacc ggccatcgtc gacctcgtgt ttcaggcgat
      361 cgtgaccctg catcggcagg gccagtcgat cctgctcgtc gagcagaacg ccgagattgc
      421 gctttcggtg tgcgactacg cctacctgat gaagcgcggc tccatcgtga tgcagggaac
      481 ggtggacgcg ctgcgcgccg acgccagcgt gctcgaggca tacctggcat gaggccggcg
      541 aacgcgggtc gccccacggg cgaggaggcg cgcgcagagc gtcgctgatc gaagcgcagg
      601 gcgatttcgg cctgcacgcg gtcggtcgcc ggtgcgcccg ctcctgaacg ttcgcctcag
      661 aacgcgacca gcgtgcgtcc gctcgtggcg ccggcgagct ggcgcccgca atgcgccggc
      721 agtgcgtcga gggcgatggt ccgcacatgc ggctcgagac actcgaagtc gggccgccac
      781 tcggacgcga gcctggccca gacgcggtgg cgcaccggcc atggcgcgtt ggcgacgacg
      841 ccgaagacct gcaccgagcg catgatgaac ggcagcacgt tgccgggata gtcgttgccc
      901 gacgcgttgc cgaccgaagc cagcgcgccg ccggacctca tcgacttcag gagccagccg
      961 agcaaggcac cgcccacgtt gtcgatggcg gccgcgaagc gctccgtctc gagcgcgcga
     1021 cggctggcga gaagcgggcc ggtttccagt acctggctgg cgccgagcgc gcgcaacgtc
     1081 gactcctgcg acggcttgcc ggtcagggcg acgacctcga agccggcgcg cgccaggatc
     1141 gcgatcgcga gcatcccgac ggcgcccgtc gcgccgtcga ccgcgacggg tccgctcgcc
     1201 ggcgtgacgc cgagctgttc gaagcggtcg agacagagcc cgaccgtgaa gccggggacg
     1261 ccgaggatcg cgatctcgcg cacgtcgagc cccgcgggca cggccatggc gtgcgccgcg
     1321 ggaacgcgca cccacggaga aaagccgccg tcgaatgcaa tgccggtgcg aaatccgtgc
     1381 gccatcaccg gcgtgccggg cgcgaaagac gcgacctccg aggcggccac gtcgccgacg
     1441 agttcgattc ccggaatacg cggatacgcc tcgatgatgc gggcgcgggc ggtgatggcg
     1501 aggcagtcct tgtagttgac gccggcgaag cgggtgcgga ccagcagctc gcccggtgac
     1561 agttcggcgg ccgtcatgct ctcgagcgcg ccgacggcag cggttctgcc gtcgctgcgg
     1621 cagcgcagcg ccgtgaatga gtccggcaag acgggcaacg cgggcaagga cgagtcggct
     1681 tcgctcatga acggaaatgg aacacatcga gccggcaggc gccaccggga gtaccgctga
     1741 tcgaaacgcc gcttcgcaac cagccgcccg aggcacgatg atcgccgctc ggcgatctcc
     1801 gcaccgcggc acgccggcct cggagaagac gatgacgcga ctgttctcgg accatcattt
     1861 gctggccctc gccgccgacg agcgtgcgct cgtcgagcgg acgcgcgcgc tcgcccgcga
     1921 gcgcatcggc cccgacgccg cgcaggtcgg cgcgcgcgac gtcttcgccc ggtcaacctt
     1981 cgatctgctc gctgccgagg g
//
LOCUS       DASRWS010000250         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3840562, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000250 DASRWS010000000
VERSION     DASRWS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3840562"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2385
                     /locus_tag="VFF72_04590"
     CDS             <1..>2385
                     /locus_tag="VFF72_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZV92470.1"
                     /translation="VGMAGRFPGAPDLRAYWDMLANGREGLTPLAENDLLTSGVPRAT
                     FGEPRYVRTAGALQGIDRFDAGFWGMSAHEASLMDPQQRILLELAWHALEDAGIDPAR
                     TDATIGVFVGTAVSTYLLFQLRDQISGPSAPSQLLSMVGNDKDYIATQLAYRLDLRGP
                     AVAVQTACSSSLVAVHLASQSLLAGECDIAIAGGVSVRVPHRVGYLYEAGGMVSPDGH
                     CRSFADDAAGTIFGSGAGLVVLRRADEAGGHRVRALLLGSAVNNDGNRKVGFTAPSQD
                     RQAAVIAEAMAIAGLRPADIGCIEGHGTATSLGDVVEVAALTAACRSAGPGAIALGSA
                     KSNIGHTETAACVAGLIKSVLMLEHATIAQSLHSDVPSTRIDWKSTPFQLARETLPWT
                     KRLVAGVSSFGIGGTNAHAIVGKAPASIGRLTAARLVISARDKAALGALTEEYRAALD
                     AKPQAFAEVAGAAARRPRHGFWVEARSASELAGAVVKEGTAPEVDTTGDGAPLALPSY
                     PFQRKRYWVDADQPLLGPAIATPFAETLHQIKLTPARLALLEQHVVEGIPILPGAFHL
                     AAFLALPRGAALSDVSFLRPMPLASLGDLQLWTKDGRLRVMTEVDGAWQELAEGRLST
                     VGAPGDAWPFDSGEAIDGATWEKILEASGLTFGPAFRSIASLERGADGVRAELGSDGT
                     RDPITLLDAGLQALGAAVAAADRGFRPASIAQLVLHRPISEARRVLARVTGDHPDVKV
                     GEAIWLDASNRIVAEARGIECRRGRSSVDRMIHRVKWVLQDDDALVAPHEALEV"
BASE COUNT          373 a          821 c          804 g          387 t
ORIGIN      
        1 ttgtcggcat ggccggccgc ttcccgggcg cgcccgatct tcgagcgtac tgggacatgc
       61 tcgccaatgg gcgagaaggc ctgacgccgc tggccgagaa cgacctgctg acctccggcg
      121 tgccgcgcgc caccttcggc gagccgcgct atgtgaggac ggccggcgcc ctgcaaggta
      181 tcgatcgctt cgacgcaggc ttctggggca tgagtgccca cgaggcctca ttgatggatc
      241 cgcagcagcg catcctgctc gagcttgcct ggcacgcgct cgaggatgcg ggcatcgatc
      301 cggcccgtac cgacgccacg atcggcgtgt tcgtcggcac tgccgtcagc acctatctcc
      361 tgttccagct gcgcgaccag atcagcggac cgagcgcgcc cagccagctg ctgtcgatgg
      421 tcggcaacga caaggactac atcgccacgc agctcgccta ccggctcgat ctgcgcgggc
      481 ccgcggtggc cgtgcagacc gcctgctcct cgtcgctggt cgcggtgcat ctcgccagcc
      541 aaagcttgct cgccggcgag tgcgacatcg ccatcgccgg cggcgtcagc gtgcgggtgc
      601 cacatcgagt cggctacctc tacgaggcgg gcggcatggt ctcgcccgac ggacactgcc
      661 gcagcttcgc cgacgacgcg gcaggcacca tcttcggcag cggcgcgggc ctggtggtcc
      721 tgcgccgcgc cgacgaagcc ggcggacatc gcgtgcgagc gttgctgctg ggctcggcgg
      781 tcaacaacga cggcaaccgc aaggtcggct tcactgcgcc aagccaggac cgacaggctg
      841 cggtgatcgc cgaagccatg gcgatcgccg gtcttcgtcc cgcggacatc ggctgcatcg
      901 aaggccacgg cacggcgacc tcgctcggcg atgtcgtcga agtggcggct ctcactgcag
      961 cttgtcgcag cgccgggcca ggcgcgatcg cccttggcag cgccaagagc aatatcggtc
     1021 acaccgagac cgccgcttgt gtcgcggggc tgatcaagtc cgtgctgatg ctggagcatg
     1081 cgacgattgc gcagagcctt cattcggatg taccgtcgac ccgcatcgac tggaagagca
     1141 caccattcca gctcgcgcgc gagacactgc cctggacgaa acgtcttgtt gccggcgtaa
     1201 gctcgttcgg catcggcggc acaaacgcgc atgcgatcgt cggcaaggca ccggcgtcca
     1261 ttggacggct cacggccgcg cgcctcgtca tctcggcgcg tgacaaggcg gccctcggtg
     1321 cgctgaccga agaatatcga gcggcactcg atgcaaagcc gcaggccttt gccgaggtcg
     1381 ccggcgccgc cgcgcgccgt ccgcgtcacg gcttctgggt cgaggcccga tcagcctcgg
     1441 agcttgccgg cgccgtcgtc aaggagggca cagctccgga agtcgacaca accggcgacg
     1501 gcgcgcctct cgcattgccg tcctatccgt tccagaggaa gcgctattgg gtcgacgccg
     1561 atcaaccgct gctcggcccg gcgatcgcga caccgttcgc cgagacgctg catcagatca
     1621 agctcacgcc ggcgcggctt gcgctgctgg agcagcatgt cgtcgagggc ataccgatcc
     1681 tgccgggcgc tttccatctc gccgccttcc tcgccctgcc gcgcggcgcc gcgctgtccg
     1741 atgtctcgtt cctgcggccg atgccgctcg cgagcctcgg cgacctgcaa ctctggacaa
     1801 aagacggtcg cctgcgagtg atgacggaag tcgacggcgc ctggcaggag cttgctgaag
     1861 gacgcctttc gaccgtcggc gcacctggcg atgcgtggcc tttcgacagt ggcgaagcca
     1921 ttgacggcgc gacttgggag aaaatcctcg aggcatccgg cctcaccttc ggacccgcgt
     1981 tcaggagcat cgcgagtctc gagcgcggcg ccgatggtgt gcgtgcggag ctcggctccg
     2041 acggaacgcg cgatccaatc acgctcctgg atgcaggcct gcaggcgctc ggggcagcgg
     2101 tcgctgcggc cgatcgcggc ttccggcctg cctcaatcgc gcagttggtg ttgcatcgcc
     2161 cgataagtga ggcgcgtcgc gttctggcgc gagtcaccgg cgatcatccg gacgtgaagg
     2221 tcggcgaggc gatctggctc gacgcgagca acaggatcgt cgcggaggct cgcggcatcg
     2281 aatgccggcg cggccgttct tcggtcgacc gcatgatcca tcgggtcaaa tgggtactgc
     2341 aagacgacga cgcactcgtg gcgccgcacg aggcgctgga agtgc
//
LOCUS       DASRWS010000251         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4132794, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000251 DASRWS010000000
VERSION     DASRWS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4132794"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFF72_04595"
     CDS             <1..207
                     /locus_tag="VFF72_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HZV92471.1"
                     /translation="GVAVEPPQTNIVFADVPAGLLDHLKSRGVLATGALYGARQRLRF
                     ATHLDVDAEGIDRTVAAIRDYLND"
     gene            220..1260
                     /gene="trpD"
                     /locus_tag="VFF72_04600"
     CDS             220..1260
                     /gene="trpD"
                     /locus_tag="VFF72_04600"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZV92472.1"
                     /translation="MSLTNVDALARTIDHREIFHDEMLSLVRRIMSGEMSPVMMAAVL
                     VGLRVKKETIGEITAAAQVMREFSAKVEVARREHLVDIVGTGGDSSHTFNISTCSMFV
                     AAACGARVSKHGNRGVSSKSGSADVLEAFGVPMNLSPAAIARSIEATGIGFMFAPNHH
                     PAMKNVAPVRKELGVRTIFNILGPLTNPADAPNILMGVFHPDLVGIQVRALQRLGAEH
                     AVVVYGKDGMDEVSLGAATMVGELKGGEILEYEIHPEDFGLTMSSNRALRVDGPAQSK
                     AMLEAVLADEPGPARDIVALNAGAALYAANVVPSIGQGLERAREAIAGGQARARLDAF
                     VAFARQSAATGQ"
     gene            1257..2045
                     /gene="trpC"
                     /locus_tag="VFF72_04605"
     CDS             1257..2045
                     /gene="trpC"
                     /locus_tag="VFF72_04605"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZV92473.1"
                     /translation="MSDILQRIVAVKRDEVAAARRRRDLASLRADAQARGDGRDFVAG
                     LRACVDAGRPAVIAEVKKASPSRGVLREDFHPGRIAASYERHGAAALSVLTDERFFQG
                     SARALEDARAAAFLPVLRKDFTIDAYQVVEARAMGADAILLIAAILDDAQLADFEAMA
                     IDLGLAVLVEVHDAVELDRATRLRTPLLGINNRDLRSFEVSLETTLALRARVPAGRVV
                     ISESGIRTPGDVARLRAGGVDAFLVGEAFMRAADPGLALADLLR"
     gene            2042..>2262
                     /locus_tag="VFF72_04610"
     CDS             2042..>2262
                     /locus_tag="VFF72_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZV92474.1"
                     /translation="MRAGELVESLSTLPPAWQAVLPGWTAERLRDVARCVAEVSGDRP
                     IAPDDPLRALRLVAPGQVRVVIVGQDPYPT"
BASE COUNT          326 a          790 c          805 g          341 t
ORIGIN      
        1 ggggttgcgg tcgagccgcc gcagaccaac atcgtctttg ccgatgtccc agccgggctg
       61 ctcgatcacc tcaagtcgcg cggcgtgctg gcgaccggcg cgctctacgg cgcccggcag
      121 cgcctgcgct tcgcgaccca cctcgacgtc gatgccgaag gcatcgaccg gacggtcgcg
      181 gcgattcgcg actacctcaa cgactgatac ggagccgtca tgtccctcac caatgtcgat
      241 gcgctggcgc gcacgatcga ccaccgcgag atcttccacg acgagatgct ctcgctggtg
      301 cgtcgcatca tgagcggcga gatgtcgccg gtgatgatgg ccgccgtcct ggtcggactg
      361 cgcgtcaaga aggaaacgat cggcgagatc accgccgcgg cgcaggtgat gcgcgagttt
      421 tcggccaagg tcgaggtcgc ccgccgcgag catctggtcg acatcgtcgg caccggcggc
      481 gactcgtcgc acaccttcaa catctcgacc tgcagcatgt tcgtcgccgc ggcctgcggc
      541 gcccgggtca gcaagcacgg caaccgcggc gtcagcagca agtcgggcag cgccgatgtc
      601 ctggaggcct tcggcgtgcc gatgaacctc tcgccggcgg cgatcgcccg ctcgatcgag
      661 gccaccggca tcggcttcat gttcgcgccc aatcaccacc cggcgatgaa gaacgtcgcc
      721 ccggtgcgca aggaactggg ggtgcggacg atcttcaaca tcctgggtcc gctgaccaac
      781 ccggccgacg ccccgaacat cctgatgggc gtcttccacc ccgatctcgt cggcatccag
      841 gtcagggcgt tgcagcggct cggtgccgag catgccgtcg tggtctatgg caaggacggg
      901 atggacgagg tctcgctcgg cgccgcgacg atggtcggcg aactgaaggg cggcgagatc
      961 ctcgagtacg agatccaccc ggaggatttc gggctgacca tgtcgagcaa ccgggcgctg
     1021 cgcgtcgacg gtccggcgca gtcgaaggcg atgctcgagg cggtgctcgc cgacgagccc
     1081 gggccggcgc gagacatcgt cgccctcaac gccggcgcgg cgctctacgc ggccaatgtc
     1141 gtgccgagca tcggccaggg tctggagcga gcccgcgagg cgatcgccgg tggccaggcg
     1201 cgggcccgcc tcgacgcctt cgtcgccttc gcgcggcagt cggcggcgac gggccagtga
     1261 gcgacatcct ccagcggatc gtcgccgtca agcgcgacga ggtggcggcg gccaggcgac
     1321 ggcgcgatct ggcatcgttg cgcgccgacg cccaggcgcg cggcgacggc cgcgatttcg
     1381 tcgccggcct gcgggcgtgc gtcgacgccg gtcggcccgc ggtcatcgcc gaggtgaaga
     1441 aggcgagccc gagtcgcggc gtcctgcgcg aagacttcca cccgggccgg atcgcggcga
     1501 gctacgagcg gcacggcgcg gcggcgctca gcgtcctcac cgacgagcgg ttcttccagg
     1561 gatcggcccg cgcgttggag gacgcgcgcg ctgccgcctt cttgccggtg ctgcgcaagg
     1621 acttcacgat cgacgcctac caggtggtcg aggcgcgggc gatgggcgct gacgcgattc
     1681 tcctcatcgc cgccatactc gacgacgccc agctcgccga tttcgaggcc atggccatcg
     1741 atctggggct ggcggtgctg gtcgaggttc acgacgctgt cgagctcgat cgcgcaacga
     1801 ggctgcgcac gcccctgctc ggcatcaaca accgcgacct gcgcagcttc gaggtgtcgc
     1861 tcgagacgac gctggccctg cgggcacgcg tgcctgccgg ccgcgtcgtc atcagcgaga
     1921 gcggtatccg cacgccgggc gacgtcgcgc ggctgcgcgc cggcggagtc gacgctttcc
     1981 tggtcggcga agcgttcatg cgagcggccg acccgggcct ggcgttggcc gacctgttgc
     2041 gatgagggcg ggcgaactcg tcgaatcgct gtcgacgctg ccgcctgcct ggcaggctgt
     2101 actgcccggc tggactgccg agcgactgcg cgacgtcgcg cgctgcgtgg ccgaggtgtc
     2161 gggcgaccgg ccgatcgcac ccgacgaccc gcttcgcgcc ttgcgactcg tggcgcccgg
     2221 gcaggtgcgg gtcgtcatcg tcgggcagga tccgtatccc ac
//
LOCUS       DASRWS010000252         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_968847, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000252 DASRWS010000000
VERSION     DASRWS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968847"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VFF72_04615"
     CDS             <1..1426
                     /locus_tag="VFF72_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZV92475.1"
                     /translation="LVVLIPVAALAVTATADAGNAPPAVHFQLDRKPSGTALTISSTG
                     PVDRGGPFFKSLGSNGRSCASCHAPEDGWSITPRSLRERFRASDGTDPVFRPVDGADS
                     PLADVSTRAARRKAYSMLLSRGVIRVGMPIPAGAQFELVGADDPYHYASAAELSLFRR
                     PLAATNLRFLSTVMWDGRETFRDAAGTDCIYNTTTCFATLHFDLADQANSATLGHAQA
                     ASPLSPGKQNAIVAFELGLTTAQVQDDDAGRLTAYGARGGPTVLQAQPFYFGINDTLV
                     GDYRSHASFTPTVMTMYDAWQLYIPDPRRPDAEGRDRGVAVARRAIARGEALFNSKPI
                     VITGVGGLNDDLGVAAIAGSCSTCHNAPNAGDHSVPLPLDIGIADASRRTADLPLYTL
                     RNKATGEIVRTTDPGRALVTGQWKDIGRFKGPTLRALASRAPYFHNGFAKTLRDVVDF
                     YNDRFGIGFSEQEKDDLVAFLKAL"
     gene            1523..2095
                     /locus_tag="VFF72_04620"
     CDS             1523..2095
                     /locus_tag="VFF72_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZV92476.1"
                     /translation="MSKPNDDHAGTAGAAAPEALARSPNDALFDTLYNELHRLARREA
                     ARGGANAPLGATTLLHEAYLDISQRQALSFPDRARFLCYAARAMRALVIDRLRANGAQ
                     KRGGGLDITSLDTETAQSIAEPGLVVEIGAALEELAVLEPALAEVVDLKFFCGFSMTE
                     IAAMQGTSERTVQRQWQKARLLLFRALRTS"
BASE COUNT          341 a          805 c          719 g          301 t
ORIGIN      
        1 cctggtcgtg ctgatcccgg tcgccgcgct ggcggtgacc gcaacggccg atgccggcaa
       61 cgcgccgcca gctgtccact tccagctcga tcgcaagccg agcggcacgg cgctgacgat
      121 cagcagcacc ggtccggtcg atcggggcgg ccccttcttc aagagcctgg gcagcaacgg
      181 ccgctcgtgc gccagctgcc acgcacccga agacggctgg tcgatcacgc cgcgcagcct
      241 gcgtgaacgg ttccgggcga gcgacggcac ggacccggtg tttcgccccg tcgacggcgc
      301 cgattcgccg ctggccgacg tgtcgacccg ggcggcgcgg cgcaaggcct acagcatgct
      361 gctgagccgc ggggtcatcc gcgtaggcat gccgatcccg gcgggagcgc agttcgaact
      421 cgtcggcgcc gacgatccct atcactatgc gtcggccgcc gagctgtcgc tgttccggcg
      481 ccctctcgcg gccaccaacc tgcgcttcct gagcacggtg atgtgggacg gacgcgagac
      541 gtttcgcgac gccgccggca ccgattgcat ctacaacacg acgacctgct tcgcgacgct
      601 gcatttcgac cttgccgacc aggcgaactc ggcgaccctc ggccacgccc aggccgcctc
      661 gccgctatcc cccggcaagc agaacgcgat cgtcgccttc gagctcggcc tgaccaccgc
      721 ccaggtgcag gacgatgacg ccggccggct gacggcctac ggcgcccgtg gcggcccgac
      781 ggtccttcag gcacagccct tctatttcgg catcaacgac acgctggtcg gcgactaccg
      841 gagccacgcg tcgttcacgc ccacggtgat gacgatgtac gacgcatggc agctctacat
      901 ccccgacccg cggcgccccg acgccgaggg acgtgaccgc ggggtcgcgg tcgcccgacg
      961 ggcgatcgcc cgcggcgagg cgctcttcaa cagcaagccg atcgtcatca ccggcgtcgg
     1021 cggactgaac gacgacctgg gcgtggccgc catcgccgga agctgctcga cttgccacaa
     1081 cgcgcccaac gccggggatc actcggtgcc cctgccgctc gacatcggca tcgccgacgc
     1141 cagccggcgc accgccgacc tgccgctcta cacgctgcgc aacaaggcca cgggcgaaat
     1201 cgtgcgcacg accgacccgg ggcgtgccct ggtgacgggc cagtggaagg acatcggccg
     1261 gttcaagggg ccgacgttgc gcgcgctcgc gtcgcgcgcc ccctacttcc acaacggctt
     1321 tgcaaagacg ctgcgcgacg tcgtcgattt ctacaacgac cgcttcggca tcggcttcag
     1381 cgagcaggag aaggacgatc tcgtggcctt cctgaaagcc ctgtgacgac cgtgcggcag
     1441 gcgtggcgga cgtccggatg cggcgagccg cgcgccgcaa gctgaggttc gcgccgtgga
     1501 gctgacgagc acaatccggc gcatgtcaaa gccgaacgac gaccatgccg ggacggccgg
     1561 ggcggccgcg ccggaagcac ttgcacgctc gccgaacgac gccctcttcg acacgctcta
     1621 caacgagctg caccgcctgg cacgccgcga ggccgcgcgc ggcggcgcga acgcgccgct
     1681 cggcgcgacg acgctcctgc acgaggcgta tctcgacatc tcgcagcgac aggcgctgag
     1741 ctttcccgac cgggcgcgct ttctctgcta cgccgcgcga gcgatgcgcg ccctggtgat
     1801 cgaccgcctg cgcgccaacg gcgcgcaaaa gcgcggtggc ggcctcgaca tcacgtcgct
     1861 cgacaccgag accgcgcaga gcatagccga gccggggctc gtcgtcgaga tcggcgcagc
     1921 gctcgaggag ctcgccgtgc tggagccggc cctcgctgaa gtggtcgacc tgaagttctt
     1981 ctgcggcttc tcgatgaccg agatcgcggc gatgcagggc acgtcggagc ggaccgtcca
     2041 gcggcaatgg cagaaggcgc ggctgctgct ctttcgcgcc ctccggacat cgtgaacatg
     2101 gttcccgacg ccgagcgctg gcggcgcctg agcccgctcc tcgacgagct cctcgacctc
     2161 gagccg
//
LOCUS       DASRWS010000253         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2981219, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000253 DASRWS010000000
VERSION     DASRWS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981219"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VFF72_04625"
     CDS             <1..1704
                     /locus_tag="VFF72_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZV92477.1"
                     /translation="ADADADGNVLVIGAPLVDSSLYPPLRGALAEAAAVVARLAGGPA
                     GIGAARVRALVSGVDDARSVINALFERPYRVVHVAGHGAPGGKGGVVLSGNTFLGPAE
                     VEAMRTVPELVFLNCCHLAARDAQSTLAPYDRAGFAANIAEALIEIGVRCVVAAGWAV
                     EDEPAEIFATTFYDALLGGGRFIDAIASARTAAWRARPGGSTWAAYQCYGDPGWTWRR
                     GGADGQGPATPLGDEFAAVSSAPALALALETIAVRLGFEGDGARSEGWLQAQRDRIRY
                     LESSFAPLWGEMGAVAEAFGLAFAEARDLDKAIEWYRRAVAAADGSASFRAAEQLGNQ
                     LARRGEAAADPAQGRVDIEEAIGRLQRLVGVQATVEREALLGSAYKRLVMNEARSLQT
                     SGATNGRKRKASPRVSAERLVALRAMARHYGNAERLARAGDVDNLFYPAKNGMSAELR
                     LAFLERRPTELALDRIEAVRESLDRAATERPDFWSVVGQIELRLLVALAARQLAPIAD
                     SLVDAFGKLKARVPSPAMWDSVHAEARFTLEPYGRVAVPAEKRAAATLLAALKAMASA
                     Q"
     gene            complement(1803..>2523)
                     /locus_tag="VFF72_04630"
     CDS             complement(1803..>2523)
                     /locus_tag="VFF72_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV92478.1"
                     /translation="GRGIGAATSRLCAARGWVVAVNYANDAAAADAIVTAIRGDGGQA
                     IAVRADVADNAAVASMFARLDSELPPLRGLVNNAGVVDLASRVDALSPARLQRMFAIN
                     VFGSFYCAREAVRRMSTKSASGRGGSIVNLSSAAARLGGAGQYVDYAAAKGAIDTFTV
                     GLAREVAGEGIRVNAVRPGIIETDIHASGGAPDRVRQMAPQLPMQRAGTAEEVAEAIV
                     WLLSDGSSYSTGSFVDVTGGR"
BASE COUNT          322 a          913 c          930 g          358 t
ORIGIN      
        1 gccgatgccg atgccgacgg caatgtgctc gtcatcggcg ccccgctggt cgattcgtcg
       61 ctgtacccgc cgctgcgcgg cgcgcttgcc gaggccgctg cggtcgtcgc gcggctggcc
      121 ggcggcccgg ccggcatcgg cgccgcccgc gtacgggcgc tggtgagcgg cgtcgacgac
      181 gcgcgctcgg tcatcaatgc gctcttcgag cggccgtatc gggtggtgca cgtcgccggc
      241 cacggcgcac cgggcggcaa gggcggcgtc gtgctgtcgg gcaacacctt cctcgggccc
      301 gccgaggtcg aggcgatgcg cacggtgccc gagctcgtgt tcctcaactg ctgccacctc
      361 gccgcgcgcg atgcgcaatc gacgctcgct ccctacgatc gcgccggctt cgccgccaac
      421 atcgccgaag cgctgatcga gatcggcgtg cgctgcgtcg tcgccgcggg ctgggccgtc
      481 gaagacgagc cggccgagat cttcgccacc accttctacg acgccctgct cggcggcggg
      541 cgcttcatcg acgcgatcgc ctcggcgcgc accgccgcct ggcgtgcccg ccccggggga
      601 agcacctggg ccgcctacca gtgctacggc gacccgggct ggacgtggcg acgcggcggt
      661 gccgacggcc agggtccggc gacgcccctc ggcgacgagt tcgcggcggt gtcgtcggcg
      721 cccgctctcg ccctcgctct cgagacgatc gcggtgcgac tcggcttcga gggcgatggc
      781 gcgcgcagcg agggctggct gcaggcgcag cgagaccgca tccgctatct cgaatcgagc
      841 ttcgcgccgc tctggggcga gatgggtgcg gtcgccgagg ccttcggtct ggcctttgcc
      901 gaggcccgcg acctcgacaa ggcgatcgag tggtatcgcc gagccgtcgc cgccgccgac
      961 ggcagcgcat cgtttcgcgc cgccgaacaa ctgggcaacc agctcgcccg gcgcggcgaa
     1021 gccgcggccg acccggcgca gggtcgcgtc gacatcgagg aagcgatcgg gcggctgcag
     1081 cgcctggtcg gcgtgcaggc caccgtcgag cgcgaagcgc tgctcggttc ggcctacaag
     1141 aggctggtca tgaacgaggc gcgctcgctg caaacgtccg gggccacgaa cggccgcaag
     1201 cgcaaggcat cgccgcgcgt ttcggccgaa cgactcgtgg ccctgcgggc gatggcgcgc
     1261 cattacggca acgccgagcg actggcccgc gcaggcgacg tcgacaacct cttctatccg
     1321 gcgaagaacg gcatgtcggc cgagctgcgg ctggcctttc tcgaacgccg gccgaccgag
     1381 ctcgctctcg accgcatcga ggcggtccgt gaatcgctcg accgcgccgc gaccgagcgc
     1441 cccgacttct ggtcggtggt cggccagatc gagcttcgcc tgctggtcgc gctggcagcg
     1501 cggcaactgg cgccgatcgc ggattcgctc gtcgatgcct tcggcaagct gaaggcacgc
     1561 gtgccgtcgc cggcgatgtg ggactcggtc catgccgagg cccgcttcac gctcgagccg
     1621 tacgggcgcg tcgccgtgcc ggcggaaaag cgcgccgcgg cgacgctgct cgcggcgttg
     1681 aaggcgatgg cgtcggcgca gtgatcgtcg ctgcgcgccg tctcggtcgt cttgacgccg
     1741 ccgcgctgcg gctcgtgccg cggcggcgcg gcgtgacggc gatccttccc gcgtaagcgg
     1801 ggctagcgtc cgccggtgac gtcgacgaag gagccggtgc tgtagctcga tccgtcgcta
     1861 agcagccaga cgatcgcttc ggccacttcc tcggccgtgc cggcacgctg catcggcaat
     1921 tgcggcgcca tctgccggac ccggtcgggc gccccgccgg aggcgtggat gtcggtttcg
     1981 atgatgccgg gacgaacggc attgacgcgg attccctcgc cggccacttc gcgcgcgagg
     2041 ccgacggtga aggtgtcgat ggcgcccttg gcggcggcgt agtcgacgta ctggccggcg
     2101 ccgccgagcc gcgccgcggc cgacgagagg ttgacgatcg aaccgccgcg gcccgaggcc
     2161 gacttcgtcg acatccgccg cactgcctcg cgggcgcagt agaagctgcc gaagacgttg
     2221 atggcgaaca tgcgctgcag gcgcgccggg ctcaacgcgt cgacgcgaga cgcgaggtcg
     2281 acgacgccgg cgttgttgac gaggccgcgc agcggcggca gctcgctgtc gaggcgcgcg
     2341 aacatcgacg ccacggccgc gttgtcggcg acatcggcgc gtaccgcgat cgcctggccg
     2401 ccgtcgccgc ggatcgcggt gacgatcgcg tcggcggcgg ccgcgtcgtt cgcatagttg
     2461 acggcgacca cccagccgcg cgccgcgcaa agccggctcg tcgccgcgcc gatgccgcgg
     2521 ccg
//
LOCUS       DASRWS010000254         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2686305, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000254 DASRWS010000000
VERSION     DASRWS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686305"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="map"
                     /locus_tag="VFF72_04635"
     CDS             <1..674
                     /gene="map"
                     /locus_tag="VFF72_04635"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZV92479.1"
                     /translation="HDYIFDVQGGVPAPLNYAPPGYTPYPKSICTSINHQVCHGIPND
                     RPLKNGDILNIDVTVIKDGWHGDTSRMFVAGDGSIAAKRLIHITYDAMWKGIMKVKPG
                     AHLGDIGNAIQTFAEGQGFSIVREFCGHGIGTRFHEEPQILHYGRPGTLDEIKPGMMF
                     TVEPMINAGKREIRETGDGWTIVTRDRSLSAQWEHTVLVTDSGYEVMTLSAGSPPPPA
                     FVTTG"
     gene            688..>2461
                     /gene="glnD"
                     /locus_tag="VFF72_04640"
     CDS             688..>2461
                     /gene="glnD"
                     /locus_tag="VFF72_04640"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZV92480.1"
                     /translation="MAPTAAAIAAPATGEAGGGVAAVRARHRDAKSELLARFARARPT
                     ARAALTLVRSLAGEVDSTLLALWQLAGMPAEASLVAVGGYGRGELYPHSDVDVLVLLP
                     DEATAGAAERHEATGRFITDCWDAGLEIGSSVRSVDECVAMARSDVTVQTALLESRLL
                     AGDRRIFAAFRKATDAAMDAKAFLRAKTLEMRQRHQKYENTPYSLEPNCKESPGGLRD
                     LQVVIWVARAAGLGRTWQQLAAYGLITPFEVRQLQRNEGVLRLIRARLHAIAGRREDR
                     LVFDLQTAVAESFGYLSNDRERASERLMRRFYWAAKAVTQLNQILMLGIEERVGGLLD
                     APMRPINDKFLEKGGMLEVASDDLYQRDPHAILETFLVYQQTVGPKGLSARTLRALYN
                     ARHLMDATFRRDPANHATFMKILKAHEGQTHAFRLMNETSVLGRYLRVFRRIVGQMQH
                     DLFHVYTVDQHILMVLRNVRRFFIPEHTHEYPFCSQLAANFDKPWLLYIAALFHDVAK
                     GRGGDHSMLGAREVRRFCRDHGVVGDDAALVEFLVVQHLTMSRIAQKEDLSDVDVIAD
                     FAASMGSERRLTALYLLTVADIRGT"
BASE COUNT          408 a          864 c          815 g          374 t
ORIGIN      
        1 cgcacgacta catcttcgac gtgcagggcg gtgtcccggc cccgctgaac tacgctccgc
       61 cgggctacac gccctacccg aagtcgatct gcacgtcgat caaccaccag gtctgccacg
      121 gcatcccgaa cgaccggccg ctgaagaacg gcgacatcct caacatcgac gtcacggtca
      181 tcaaggacgg ctggcatggc gacacgagcc gcatgttcgt cgccggcgac ggctcgatcg
      241 cagcgaagcg gctgatccac atcacctacg acgcgatgtg gaagggcatc atgaaggtga
      301 agccgggtgc tcatctggga gacatcggca acgccatcca gaccttcgcc gagggccagg
      361 gcttctcgat cgttcgcgag ttctgcggcc acggcatcgg cacccgtttc cacgaggagc
      421 cgcagatcct ccactacggc cggcccggca cgctcgacga gatcaagccg ggcatgatgt
      481 tcacggtcga gccgatgatc aacgccggca agcgcgagat ccgcgagacg ggcgatggct
      541 ggaccatcgt cacgcgcgac cggtcgctgt cggcgcagtg ggagcacacg gtgctcgtca
      601 ccgacagcgg ctacgaggtg atgacgctgt cggccggctc gccgccgccg ccggccttcg
      661 tcaccaccgg ctgagccgtc gtccgcgatg gcgccgacgg cagcagcgat cgcggcgcct
      721 gccacgggcg aagcgggcgg cggcgttgcc gccgtccggg cccgccatcg cgacgccaag
      781 tccgagctgc tcgcccgctt cgcccgggca cgaccgacgg cgcgagccgc gttgacgctg
      841 gtgcgctcgc tcgccggcga ggtcgactcg acgctgctcg cgctctggca actggcgggc
      901 atgcccgcgg aagcgtcgct ggtggcggtc ggcggctacg ggcgcggcga gctctatccg
      961 cactccgatg tcgacgttct cgtcctgctc ccggatgaag cgactgcggg cgcggccgag
     1021 cgccacgagg cgaccggccg attcatcacc gactgctggg acgccgggct cgagatcggc
     1081 tcgagcgttc gcagcgtcga cgaatgcgtg gcgatggcac gaagcgacgt cacggtgcag
     1141 acggcgttgc tcgagtcgcg cctgctggcc ggcgaccggc gcatcttcgc ggcgttccgc
     1201 aaggccaccg atgcggcaat ggacgcgaag gcctttctcc gcgccaagac gctcgagatg
     1261 cgccagcgcc accagaagta cgagaacacg ccctattcgc tggagccgaa ctgcaaggaa
     1321 agccccggcg gcctgcgcga cctgcaggtc gtgatctggg tggcgcgggc cgcgggcctc
     1381 ggccgaacct ggcagcagct ggccgcatac ggcctgatca cgccgttcga ggtgcgccag
     1441 ctgcaacgca acgaaggcgt gctgcggctg atccgcgcgc gactgcacgc gatcgccggg
     1501 cgacgcgagg atcgcctcgt cttcgacctg cagacggccg tcgccgagag cttcggctac
     1561 ctgtcgaacg accgcgagcg tgcgtcggag cgactgatgc gccgcttcta ctgggcggcc
     1621 aaggcggtca cccagctcaa ccagatcctg atgctcggca tcgaggagcg ggtcggcggc
     1681 ctgctggatg cgccgatgcg tcccatcaac gacaagttcc tcgagaaggg tggcatgctc
     1741 gaggtcgcga gcgacgatct ctaccagcgc gacccgcacg ccatcctcga gacgtttctc
     1801 gtctaccagc agaccgtggg gccgaagggg ctctcggcgc ggacgctgcg cgccctctac
     1861 aacgcgcgtc acctgatgga cgcgacgttt cgccgcgatc ccgccaacca tgcgaccttc
     1921 atgaagatcc tgaaggcgca cgaaggccag acgcacgcct tccggctgat gaacgagacg
     1981 agcgtgctcg gccgctacct gcgggtcttt cgccgcatcg tcggccagat gcagcacgac
     2041 ctgttccacg tctacacggt cgaccagcac atcctgatgg tgctgcgcaa cgtgcgccgc
     2101 ttcttcattc ccgagcacac ccacgagtac ccgttctgct cgcagctggc ggccaatttc
     2161 gacaagccgt ggctgctgta catcgccgcg ctctttcacg acgtggccaa ggggcgcggc
     2221 ggcgaccatt cgatgctcgg ggcgcgcgag gtacgccggt tctgtcgcga tcacggcgtc
     2281 gtcggcgacg atgcggcgct ggtcgaattc ctcgtcgtcc agcacctgac gatgtcgcgc
     2341 atcgcccaga aggaagatct ctcggacgtc gacgtcatcg ccgacttcgc cgcgtcgatg
     2401 ggcagcgagc ggcggctgac cgcgctctac ctgctgaccg tcgccgacat ccgcggcacc
     2461 a
//
LOCUS       DASRWS010000255         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3279894, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000255 DASRWS010000000
VERSION     DASRWS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3279894"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFF72_04645"
     CDS             <1..643
                     /locus_tag="VFF72_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZV92481.1"
                     /translation="AASFSTPTLFVLAEDLSSMQLRVNVDEADVGQVHPGQHATFTVD
                     AYPGRAFPATVERIDLASTNTASTAASSTGQASASGQTNSVVDYEARLTVANPNGLLR
                     PGMTATATIATETTAASLLVPNGALRFSPEDANKPQKGAGLQVQVGLQQEEQKATIGV
                     GSRQHVYVLKDDGTLQKIDVVVGLSDGRMTAVTSSQLKAGMKVVTGIKAKEKK"
     gene            651..1367
                     /locus_tag="VFF72_04650"
     CDS             651..1367
                     /locus_tag="VFF72_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92482.1"
                     /translation="MTGEPLIELTGITKTYGQGTAAFQALKGVDLGISQGEFAAIMGA
                     SGSGKSTMMNILGCLDSPTTGVYRFMGVEVQSMTKDQRSLLRRRYLGFVFQGFNLLAR
                     TSALENVELPLLYRGEKKTARREAAMKALDLVGLGPWADHTPAELSGGQQQRVAIARA
                     LVTEPEVLLADEPTGNLDSERSIEIMELLAELNRGGITVLMVTHEGEMAAFARTIVHF
                     RDGLIERVERGHRAETPVSG"
     gene            1367..2566
                     /locus_tag="VFF72_04655"
     CDS             1367..2566
                     /locus_tag="VFF72_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92483.1"
                     /translation="MFGATLLLALRQIRRHLLRSSLTTIGIIIGVAAVITMVTLGNGL
                     TAQIQKQISALGSDVLIAFPQQTDQNRQPRPFKQDDVIAVREQLAGVKSVAGEVNKNV
                     HAFHNGQTWSSKVEGVSDAFMDARGIGMDQGRRFTPAEEQSGANVCIIGPKVQQEIFV
                     EGVSPLGEQVRLDDVSCTVIGVFASRAQGGNNQDDDNSVFVPLKNAQRRFTGADTIGY
                     VVISYDPAYSGAAIQQALIDLLRERHRLQPGEPNDFNIIDSAALNDTVGSVVARLTVA
                     VAAIAGISLLVGGVGIMNIMLVSVTERTREIGIRLAIGALAREVRLQFLTEAVVLCCF
                     GGLIGIILALGLSFALAKAIDVPFVFSVSVNVLSFAFSALMGIIFGYYPARRASRLDP
                     IEALRHE"
BASE COUNT          471 a          912 c          892 g          425 t
ORIGIN      
        1 cgccgcctcg ttcagcactc ccacgctgtt cgtgctggcg gaagacctct cgagcatgca
       61 actgcgggtc aacgttgacg aagccgatgt cggccaggtc caccccggcc agcacgcgac
      121 cttcacggtc gatgcctatc ccggtcgcgc cttcccggcg acggtcgagc ggatcgatct
      181 cgcttcgacc aataccgcca gcacggcagc ctccagcacc gggcaggcat ccgccagcgg
      241 gcagacgaat tcggtggtcg actacgaagc ccgactgacc gtcgccaacc cgaacggcct
      301 gttgcggccg ggcatgaccg cgacggcgac catcgccacg gagaccaccg ccgccagcct
      361 gctcgtcccc aacggcgcgc tgcgcttcag ccccgaggac gcgaacaagc cgcagaaggg
      421 tgccggcctg caagtgcagg tcggcctgca gcaggaagag cagaaggcga cgatcggcgt
      481 cggcagccgc cagcacgtgt acgtgctcaa ggacgacggc acgttgcaga agatcgacgt
      541 ggtcgtgggc ttgagcgacg gccggatgac ggcggtcacc tccagccagc tcaaggcggg
      601 catgaaggtc gtcaccggca tcaaggccaa ggaaaagaag tgagccaccc gtgaccggcg
      661 aaccgctcat cgagctgacg gggatcacca agacctacgg ccagggcacg gccgcgttcc
      721 aggcgctgaa gggcgtcgac ctggggatct cccaaggtga gttcgcggcg atcatgggcg
      781 cgtctgggtc gggcaagtcg accatgatga atatcctcgg ctgcctcgat agcccgacga
      841 ccggcgtcta tcggttcatg ggcgtcgagg ttcaatcgat gaccaaggac cagcgctcgc
      901 tcctccggcg acgctatctc gggttcgtct tccagggctt caatctgctg gcccggacga
      961 gcgcgctcga aaacgtcgag ctgccgctgc tctaccgcgg tgagaagaag acggcccggc
     1021 gcgaagccgc gatgaaggcg ctcgacctcg tcggcctcgg cccctgggcc gatcacacgc
     1081 cggccgagct ttccggcggc cagcagcagc gcgtggcgat cgcccgcgcg ctggtgaccg
     1141 agcccgaagt cctgcttgcc gacgagccga ccggcaacct cgacagcgaa cgctcgatag
     1201 agatcatgga gctgctcgcc gagctgaacc gcggcgggat caccgtgctg atggtcaccc
     1261 acgaaggaga gatggcggcg ttcgcgcgca ccatagtcca cttccgcgac ggtctgatcg
     1321 aacgcgtaga gcgcggccat cgcgcggaaa ccccggtgag cggctgatgt tcggcgcgac
     1381 gctcctgctg gcgttgcggc agatccgccg ccatctgctg cgctccagcc tgacgacgat
     1441 cggcatcatc atcggcgtgg ccgcggtgat cacgatggtc acgctgggca acggcctgac
     1501 cgcgcagatc cagaagcaga tctccgcgct cggctcggac gtgctgatcg cctttccgca
     1561 gcagaccgac cagaaccgcc agccgcgccc gttcaagcag gacgacgtca tcgcggtccg
     1621 cgagcagctc gccggggtca aatcggtcgc cggcgaggtc aacaagaacg tccacgcgtt
     1681 ccacaacggc cagacctgga gctcgaaagt cgaaggcgtc agcgacgctt tcatggacgc
     1741 gcgcgggatc ggcatggacc agggccgccg ctttaccccg gccgaagagc aatcaggcgc
     1801 caacgtctgc atcatcggcc ccaaggtgca acaggagatt tttgtcgaag gagtgagccc
     1861 gctcggtgag caggtgcggc tcgacgacgt ttcctgcacg gtgatcggcg tcttcgctag
     1921 ccgcgctcaa ggcggcaaca accaggacga cgacaattcg gtgttcgtgc ccttaaagaa
     1981 cgcccagcgg cgcttcaccg gcgcggacac gatcggctac gtcgtcatct cctacgatcc
     2041 cgcctactcc ggggccgcta tccagcaggc gctgatcgac ctgctccgcg agcgccaccg
     2101 cctgcagccc ggcgagccca acgacttcaa catcatcgat tccgccgctc tcaacgacac
     2161 cgtcggcagc gtcgtcgcgc gcctgacggt ggcggtcgcg gctattgccg ggatcagcct
     2221 gctggtcggc ggcgtcggga tcatgaacat catgctggtc tcggtgaccg agcgcacgcg
     2281 ggagatcggc atccgcctcg cgatcggcgc gctcgcgcgg gaagtgcgcc tgcagtttct
     2341 caccgaggcg gtggtgctgt gctgcttcgg cgggctgatc gggatcatcc tcgcgcttgg
     2401 cctgtcgttc gccctggcca aggcgatcga cgtgccgttc gtgttcagcg tgtcggtcaa
     2461 cgtgctgagc ttcgcgttct cggcgctgat gggcatcatc ttcgggtatt atccggcgcg
     2521 gcgcgcctcg cgcctggacc cgatcgaggc gctgcgccac gaatagcccg cgcggggctt
     2581 tgcgccggcg tcgatttcgg tcaaggattg cttcgtcgcg cccgctattg gggcctgatc
     2641 gctcgtaccg agcgccgggg gcgaaggaga ggatggcgga aaacgattcc gggctggccg
//
LOCUS       DASRWS010000256         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2686571, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000256 DASRWS010000000
VERSION     DASRWS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686571"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /gene="glmS"
                     /locus_tag="VFF72_04660"
     CDS             complement(<1..1799)
                     /gene="glmS"
                     /locus_tag="VFF72_04660"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZV92484.1"
                     /translation="MCGIVAAVSSRNIVPLLVEGLRRLEYRGYDSCGVALHQAGRLRR
                     ARSTARVAELQANVSAEHLEGGTGIAHTRWATHGAPVVHNAHPHFSHGPGVTDAGEAS
                     GMAASGRVGLVHNGIIENHDELRGELRARNYHFATQTDSEVIAHLVDMYYDGDLLEAV
                     ERTLPRLRGAYAIAVFCRDEPHRVIGARSGSPLVLGVGGAAGASENFLASDAMALAGV
                     TDQIAYLEEGDVVDLQLGKYWVVRADATGTFRRVDRAVRTVLAHSGAAELGPYRHYMQ
                     KEIFEQPKAIADTLDAVPGVSPDLFGDGAHRIFREIEQVLILACGTSFYSGSVAKYWL
                     EGLARIPTTVEVASEYRYRDSVPNPKTLVVTISQSGETADTLAALRHARSLGMAHTLT
                     ICNVQTSAMVRECAMAYVTRAGVEIGVASTKAFTTQLVGLFLLALSLAQVRGRLDDEA
                     EAKHLKALRHLPVAVQSVLALEPQVIAWSEEFARKENALFLGRGMHYPVALEGALKLK
                     EISYIHAEAYPAGELKHGPLALVTAQMPVVAIAPNDALLEKLESNLQEVRARGGELFV
                     FADARTRIESGEGVHVIRMPEHYGALSPILHVVP"
     gene            1935..2456
                     /locus_tag="VFF72_04665"
     CDS             1935..2456
                     /locus_tag="VFF72_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZV92485.1"
                     /translation="MSIESVVPFEVDGVDLRILDALQQDASLTNQALAERVHTSAATC
                     LRRVKRLVDSGVIERRVALLSPQRMGHGLTAIVEVTLDRQSAESLAAFEARAVAEDAV
                     QQCHRVSPGPDFILVVHVADMPAYHDLVQRLLTADANVRNVKAFFSVHRAKFETRVAL
                     PRAVTATGRSAAS"
     gene            complement(2469..>3153)
                     /locus_tag="VFF72_04670"
     CDS             complement(2469..>3153)
                     /locus_tag="VFF72_04670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV92486.1"
                     /translation="AGQDGQHATCRVIREHGWRFGLDFATGHKTGFYLDQRDNRKRFA
                     DIVRQLGLRRVLNAYCYSGGFSVAALAGGAEHVTSVDSSAPALALAESNVVLNGFDPS
                     RHEAVDADVNRTLRQRIDAGQRFDAIVLDPPKLAPTVAHAERAARAYKDINRLALSLL
                     RPGGVLFTFSCSAAITAELFQKIVAGAGADAGVDGAVIAHLHAAADHPASLRFPEGEY
                     LKGLAIVRR"
BASE COUNT          497 a         1056 c         1094 g          506 t
ORIGIN      
        1 ggcacgacgt gcaggatcgg cgagagcgcg ccgtagtgct cgggcatgcg gatgacgtgc
       61 acgccctcgc ccgattcgat gcgggtgcgc gcgtcggcga agacgaagag ctcgccgccg
      121 cgggcgcgca cttcctgcag gttgctctcg agcttttcga gcagcgcgtc gttgggcgcg
      181 atggcgacca ccggcatctg cgccgtcacc agcgcgagcg gaccgtgctt caactcgccc
      241 gcggggtagg cctcggcatg gatgtagctg atctccttga gcttcagcgc gccctcgagg
      301 gcgaccggat agtgcatgcc gcggccgagg aagagcgcgt tttccttgcg cgcgaactcc
      361 tcgctccagg cgatcacctg cggctcgagc gcgagcaccg attgcaccgc caccggcagg
      421 tggcgcaggg ccttcaggtg cttcgcctcg gcctcgtcgt cgagccggcc acgcacctgg
      481 gcaagcgaga gcgccagcag gaagaggccg acgagctgcg tggtgaacgc cttcgtcgag
      541 gcaacgccga tctcgacgcc ggcccgcgtc acgtaggcca tcgcgcattc gcgcaccatg
      601 gcgctggtct gcacgttgca gatggtgagc gtgtgcgcca tgccgagcga gcgggcgtgc
      661 ctgagcgcgg ccagcgtatc ggccgtctcg ccgctttgcg agatcgtcac caccagggtc
      721 ttcggattcg gcacgctgtc gcgatagcgg tattcgctcg ccacctcgac ggtggtcgga
      781 atcctcgcca gcccctcgag ccagtacttg gcgaccgagc cgctgtagaa gctcgtgccg
      841 caggcgagga tcagcacctg ctcgatctct cgaaagatcc ggtgagcgcc gtcgccgaag
      901 aggtcgggcg acacgccggg cacggcgtcg agggtgtcgg cgatcgcttt cggctgctcg
      961 aagatctcct tttgcatgta gtgccggtaa ggcccgagct cggccgcgcc gctgtgcgcc
     1021 aggaccgtgc gcacagcgcg atcgacgcgg cgaaacgtgc cggtggcgtc ggcgcgaacg
     1081 acccagtact tgccgagctg caggtcgacg acgtcgcctt cctcgagata ggcgatctgg
     1141 tcggtgacgc ccgccagcgc catcgcgtcg gaggcgagga agttctcgct cgcaccggcc
     1201 gcgccgccca cgcccagcac cagcggcgag ccgctgcgtg cgccgatcac gcggtggggc
     1261 tcgtcgcggc agaagaccgc gatcgcatag gcgccgcgca ggcgcggcaa ggtacgctcg
     1321 acggcctcga gcaggtcgcc gtcgtagtac atgtcgacga gatgggcgat gacctcgctg
     1381 tcggtctgcg tggcgaagtg gtagttcctg gcgcgcagct cgccgcgcag ctcgtcgtgg
     1441 ttctcgatga tgccgttgtg gacgagcccg acgcggcccg acgcagccat gccggaagct
     1501 tcgccagcgt cggtgacgcc aggcccgtgc gaaaagtgcg gatgcgcgtt gtgcacgacc
     1561 ggcgcgccgt gcgtggccca gcgggtgtgg gcgatgccgg tgccgccctc gagatgctcg
     1621 gccgagacgt tcgcctgcag ctcggcgacc cgcgcggtgc tgcgcgcgcg gcggagacgt
     1681 cccgcctgat gcagcgccac cccacacgag tcgtagccgc gatattcgag ccgccgaagg
     1741 ccttcgacga gcagcggaac gatgtttcgg gagctgacgg cggcgacgat tccacacatg
     1801 gcgttgggct ttcggtgtca gggaaggggc gactgtacga agatcctaga gaatttatct
     1861 ctcataatcg gctcgatctg gacgtacgat ttccttgatc tgacgttctg aaatatttgc
     1921 atcaataatc tccaatgtcg atcgaatcag tcgtcccctt cgaggtcgat ggcgtcgacc
     1981 tccgcatcct cgacgccctc cagcaagacg cctcgctgac caaccaggcc ctcgcggagc
     2041 gcgtccacac ctccgcggcg acatgcctgc gacgggtcaa gcggctcgtc gattcgggcg
     2101 tcatcgaacg gcgcgtcgcc ctgctctcgc cgcagcgaat gggccacggc ctgacggcga
     2161 tcgtcgaggt gacgctcgac cggcaatcgg ccgaatcgct cgcggccttc gaggcgcgcg
     2221 ccgtcgccga agacgcggtg cagcaatgcc atcgcgtatc gcccggcccc gacttcatcc
     2281 tcgtcgttca cgtcgccgac atgccggctt accacgatct ggtgcagcgc cttctcaccg
     2341 ccgatgccaa cgtccgcaac gtcaaggcct tcttcagcgt gcatcgggcc aagttcgaga
     2401 cgcgcgtcgc cctgcctcgg gccgtgacgg cgaccggccg gtcggcggcc tcgtgaagcc
     2461 aggagcgatc aacgcctgac gatcgcgagg cccttcagat actcgccttc cgggaagcgc
     2521 agcgacgccg gatggtcggc agccgcgtgc aggtgggcga tcacggcgcc atcgacaccc
     2581 gcgtcggcgc cggcgccggc gacgatcttc tggaacagct cggccgtgat cgccgccgag
     2641 cacgagaacg tgaagaggac gccacccggg cgcagcagcg acagcgcgag acggttgatg
     2701 tccttgtagg cgcgagccgc gcgttcggcg tgggcgaccg tcggagcgag cttcggcgga
     2761 tcgaggacga tcgcatcgaa gcgctggccg gcgtcgatcc gctggcgcaa ggtccggttg
     2821 acgtctgcat cgaccgcctc gtgtcgcgac gggtcgaagc cgttcagcac cacattggac
     2881 tcggccagcg ccagtgccgg cgccgaggaa tcgacgctcg tcacgtgctc ggcgccgccg
     2941 gcaagcgcgg cgacgctgaa tccgccgctg tagcaatagg cattgagcac gcggcgaagg
     3001 ccgagctggc ggacgatgtc ggcgaagcgc tttcggttgt cgcgctgatc gagatagaag
     3061 ccggtcttgt ggccggtcgc gaagtcgaga ccgaagcgcc agccgtgctc gcggatgacg
     3121 cgacaggtgg cgtgttggcc gtcctggccg gca
//
LOCUS       DASRWS010000257         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2395642, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000257 DASRWS010000000
VERSION     DASRWS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395642"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="dusA"
                     /locus_tag="VFF72_04675"
     CDS             <1..714
                     /gene="dusA"
                     /locus_tag="VFF72_04675"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HZV92487.1"
                     /translation="ERVQRGAFGACLMAEPALVAECVKAMRDAVSIPVTVKHRVGIDR
                     TEEYGFVRDFVGGVSEEGGCTTFIVHARNAWLHGLSPKQNREIPPLRPELVLRLAQDF
                     PDIAFVINGGIAGDAAIEGHLHRLDGVMVGRAAYHEPFGMANWDQRFFAAPDDPVDRD
                     AVEAAFVEYMSAMAASGEPWSHASRHMLGLRNATPGARRWRQVWSDHRLHGLPPREVS
                     RRARAALAAPEVSIEPALA"
     gene            872..1531
                     /locus_tag="VFF72_04680"
     CDS             872..1531
                     /locus_tag="VFF72_04680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV92488.1"
                     /translation="MANRRTAIDDEVYDYVVAHGVRETTAQVGLRAATRSVPHAGMQI
                     GPDQAQFMALLVRLIGARRAIEIGVFTGYSALAVALAMPEDGYLLACDVSEPWTSVGK
                     PFWAEAGVAVKIDLRLGPALRTLDAEIAKGGKASFDFAFIDADKSGYDAYFERCMELL
                     RPGGLIAIDNVLWGGAVAHPSKDPDTAALQALNDKLHADARIDLSLLPIGDGLTLARK
                     R"
     gene            1572..1937
                     /locus_tag="VFF72_04685"
     CDS             1572..1937
                     /locus_tag="VFF72_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV92489.1"
                     /translation="MNHVTIVGEDRTRTLDFYVGLLGLREGHRPDLGFAGAWLYGAPA
                     EAVLHIYFDRSVPEPRTGVIDHVAFSASDLRSVKARFDARGVSYRLQRQRGSGHWQLF
                     TVDPSGVKVELDFDAAEAT"
     gene            complement(1996..2901)
                     /locus_tag="VFF72_04690"
     CDS             complement(1996..2901)
                     /locus_tag="VFF72_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZV92490.1"
                     /translation="MKPIPWLSYLCLAAAMAVVGAYVGFSKALVIVFPIFVLAWLRFA
                     IAAVAMLPWLRRPADEPALDGRTRGLLFLESFFGNFLFSICMLFGIARSTALAAGVIM
                     AAIPAVVALLSWVLLRERVHWRTGLGIAFAMAGIALVSMTRDDDGEFDRGSLVGNLLL
                     FGAVTCEALYVVLGKRLSARLGPKRISSLINLWGLALVTPLALWQLPQFHAGYIGGSF
                     WALLVAYALLASVATVWLWMTGLKRVPASSAGVFTVFLPISAAAVGVLFLGEQFRPIQ
                     LAAFALALAGVVLAAWHRDASAVPG"
     gene            complement(2898..>3353)
                     /locus_tag="VFF72_04695"
     CDS             complement(2898..>3353)
                     /locus_tag="VFF72_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV92491.1"
                     /translation="GFPERGMLTIWMGHLLLGMAYATVVVRARLQTLNPQLEEAAMDL
                     GARPWQVFWLVTLPMIGQALLSAFLLTFTLSLDDVVLSAFLSGPGATTMPLVIFSRAR
                     LGLNPSVNAVATIIIAVVAIGVVVASFLIARGERRRAADVTAANRALPA"
BASE COUNT          579 a         1117 c         1185 g          472 t
ORIGIN      
        1 gagcgcgtgc agcgcggcgc gttcggcgcc tgcctgatgg ccgagcccgc gctcgtcgcc
       61 gagtgcgtca aggcgatgcg cgacgcggtg tccatcccgg tcaccgtcaa gcaccgcgtc
      121 ggcatcgacc gcaccgagga atacggcttc gtgcgcgact tcgtcggcgg cgtcagcgaa
      181 gagggcggct gcaccacctt catcgtccat gcccgcaacg cctggctgca cgggctgagc
      241 cccaagcaga accgcgagat cccgccgctg cggccggaac tcgtcttgcg cctggctcag
      301 gatttcccgg atatcgcctt cgtgatcaac ggcgggatcg ccggcgacgc cgcgatcgaa
      361 ggccatctcc accggctcga cggggtcatg gtcggccgtg cggcgtatca cgagccgttc
      421 ggcatggcga actgggacca gcgcttcttc gcggcgcccg acgatcctgt ggatcgtgac
      481 gctgtcgagg ccgcgttcgt cgagtacatg tcggcgatgg ccgcgtccgg cgagccgtgg
      541 tcgcatgcgt cgaggcacat gctgggcctg cgcaatgcaa cgcccggcgc gaggcgctgg
      601 cgccaggtgt ggtccgacca tcgactgcac gggctgcctc cgcgcgaggt ctcgcgacgc
      661 gcccgtgccg cgctggccgc gccggaggtg agcatcgaac cggcgctggc ctgatcccct
      721 ccggacggcc tctttcaccg gagggcagca gaacggtgtt cgcattgccc gacaattcat
      781 cgcgttcgtc gcgagcgcga aagcatcggg caacgcggat cgtgcgccgc gctttcgatc
      841 gggaaaacgc aagcaacgcg gaggagccga catggcgaat cgaagaacag cgatcgatga
      901 cgaggtctac gactacgtcg tcgcgcacgg tgtgcgtgaa acgacggcgc aggtcggcct
      961 gcgcgccgcg acccgcagcg tgccgcatgc cggcatgcag atcggtcccg accaggcgca
     1021 attcatggcg ctgctcgtgc ggctgatcgg agcgcggcgg gcgatcgaga tcggcgtgtt
     1081 caccggctac agcgcccttg cggtggcgct ggcgatgcca gaggacggct atcttctggc
     1141 ctgcgatgtc agcgagccct ggacctccgt cggcaagccg ttctgggccg aagcgggcgt
     1201 cgccgtcaag atcgacctgc ggctcgggcc cgcgttgcgc acgctcgatg cggagatcgc
     1261 caaaggcggc aaggcaagct tcgacttcgc attcatcgac gccgacaagt ccggctacga
     1321 cgcgtatttc gagcgttgca tggagctgct gcgcccgggc ggcctgatcg cgatcgacaa
     1381 cgtcctctgg ggcggcgcgg tggcccaccc gtcgaaggat cccgacaccg cggccctgca
     1441 ggcgctcaac gacaagctgc acgccgatgc ccggatcgac ctgtcgttgc tgccgatcgg
     1501 cgatgggctg acgctggcgc gcaagcgctg atcgaccgcg tgttggccaa gaggtctcga
     1561 tgatcgaggg aatgaatcac gtcaccatcg tgggcgagga ccggacgcgc acgctggact
     1621 tctatgtcgg cctgctcggt cttcgcgaag gccatcgacc cgatctcggg ttcgccggtg
     1681 catggctcta cggcgctccg gccgaggccg tgctgcacat ctacttcgac cgctccgtcc
     1741 cggaaccgcg caccggcgtc atcgatcacg tcgccttctc ggcaagcgat ctgcgctcgg
     1801 tcaaggcgcg tttcgatgcg cgcggtgtct cgtatcggct gcagcgccaa aggggctccg
     1861 gccactggca gctcttcacc gtcgatccga gcggcgtcaa ggtcgagctc gatttcgacg
     1921 ctgcggaggc gacataaccg ttgccggcgc ggtggccgcc ggtgccgaac ggtgttttcg
     1981 tcgcaggccc gtggcctagc ccggcaccgc gctcgcgtcg cgatgccagg cggcgagcac
     2041 gacaccggcc agcgccagcg cgaaagcggc gagctggatc ggcctgaact gttcgccgag
     2101 gaagagcacg ccgaccgcgg ccgcgctgat cggcaggaac accgtgaaga cgcccgccga
     2161 tgaggccggc acgcgtttga gaccggtcat ccacagccag accgtggcga cgctggcgag
     2221 cagcgcgtat gccaccagca acgcccagaa cgatccgccg atgtagcccg cgtggaactg
     2281 gggcagctgc cagagtgcaa gcggtgtgac cagggcgagg ccccagaggt tgatcagcga
     2341 gctgatccgt ttcgggccca ggcgcgccga aagcctcttg ccgagaacga cgtagagcgc
     2401 ctcgcaggtc accgcgccga acagcagaag gttgcccacc agtgagccgc ggtcgaactc
     2461 gccatcgtcg tcgcgggtca tcgacacaag ggcgatgccg gccatcgcga aggcgatgcc
     2521 cagcccggtc cgccagtgca cgcgctcgcg caggagaacc cacgacagca gcgcgaccac
     2581 cgccgggatc gccgccatga tcacgcccgc cgcgagcgcg gtgctgcggg cgatgccgaa
     2641 gagcatgcag atcgagaaga ggaaattgcc gaagaaggac tcgaggaaca gcagcccgcg
     2701 ggtgcggccg tcgagcgccg gctcgtcggc gggtcgacgc agccacggca gcatcgcgac
     2761 ggcggcgatc gcgaagcgca gccatgccag aacgaagatc ggaaagacga tcacgagggc
     2821 cttcgagaag ccgacgtagg ctccgacgac cgccatcgcc gcggcgaggc aaagatacga
     2881 cagccagggg atgggtttca tgcggggagg gcccggttgg cggcggtgac gtcggcggcg
     2941 cgtcgccgct cgccgcgggc gatgaggaag ctggccacga cgaccccgat cgcgacgacg
     3001 gcgatgatga tcgtcgccac ggcgttgacg ctcggattga ggccgagccg ggcgcgcgag
     3061 aagatgacca gcggcatcgt tgtcgctccg gggccggaga ggaaggcgga gaggacgacg
     3121 tcgtcgagcg acagcgtgaa ggtcagcagg aaggccgaga gcagcgcctg gccgatcatc
     3181 ggcagcgtca cgagccagaa cacctgccag ggccgggcgc cgagatccat cgccgcctcc
     3241 tcgagctgcg ggttgagcgt ctgcaggcgg gcgcgaacga cgacggtggc gtaggccatg
     3301 ccgagcagca aatggcccat ccagatcgtc agcatgccgc gctccggaaa gcc
//
LOCUS       DASRWS010000258         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3282795, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000258 DASRWS010000000
VERSION     DASRWS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282795"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFF72_04700"
     CDS             complement(<1..640)
                     /locus_tag="VFF72_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92492.1"
                     /translation="MQDILRVSKLSKTYASGFTALRNIDLAIGRGEIFALLGPNGAGK
                     TTLISIVCGIVNASSGSVSVAGHDIVADYRAARTMIGLVPQELSTDAFESVWSTVSFS
                     RGLFGKPKNPAHIEKVLKDLSLWDKKDNKIITLSGGMKRRVMIAKALSHEPQILFLDE
                     PTAGVDVELRKGMWEVVRALQASGVTIILTTHYIEEAEEMADRIGVINKGEII"
     gene            765..2087
                     /locus_tag="VFF72_04705"
     CDS             765..2087
                     /locus_tag="VFF72_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZV92493.1"
                     /translation="MPAADLTEEQARTYMHKLLAAMSQAGGSDLFIAADFPPSMKANG
                     NMQPLSQQKLGAEATRMLAHALMNEAQREEFDKEMECNFAISIPGVSRFRVNVHMQQR
                     CVSMVIRTIASEIPSFEKLKLPETLKEVVMTKRGLVLVVGATGSGKSTTLAAMIDHRN
                     RASAGHIITVEDPVEYVHVSQKSLVTHREIGIDTKSWHHALKNTLRQAPDVILIGEIR
                     DAETMEHAIAFAETGHLCLGTLHANSASQTIERIINFFPDERRQQLLMDLSANLRAIV
                     SQRLVRREDGKGRSAAIEILLNTPSIAEKIFQSAFHEIKGLMAKSRELGMCTFDAALF
                     DLYNDGAISFDEAIRNADSANELRLNIKLKSKRGEPKQSAFGGLSIDPMTQPADLEER
                     AHQEMLKKQESRRRFEEEQLRQLREKKRLAEEAAMATKGPVLPGSMSN"
     gene            complement(2128..2844)
                     /locus_tag="VFF72_04710"
     CDS             complement(2128..2844)
                     /locus_tag="VFF72_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZV92494.1"
                     /translation="MATDAPVTAGPDAGAIALSVPVEAHGAVPALLLRPPSARACYVF
                     AHGAGAGMRHGFMAAFAAGLAARHIATLRFDFPFMARGSRRPDPPSLAHATVRGAVRT
                     AAEHCAGLPLFAGGKSFGGRMTSQAQALAPLPGVLGLVFVGFPLHPAGKPSNERAAHL
                     DKVGLPMLFLQGTRDELAAPELLLPVIGQLGGRATLAWFDDADHAFHVRARSGRTDAE
                     VLQAMLDKTSNWIGERAVAS"
     gene            3084..3320
                     /locus_tag="VFF72_04715"
     CDS             3084..3320
                     /locus_tag="VFF72_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92495.1"
                     /translation="MMTPTPEILDFDAWCERCARRLGEIYDQLTIAEARRLARDVYAF
                     ERTRAMDPSRAAEFVAVEMTRPDRGPFERRAVAR"
     gene            complement(3358..3909)
                     /locus_tag="VFF72_04720"
     CDS             complement(3358..3909)
                     /locus_tag="VFF72_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92496.1"
                     /translation="MKYPVPPPTVAVMTGIETKKVLFIRQRSERWQSFERRAGLAEFT
                     RRFVGFARPMVVVVPDFSRFEAKFQQTFVYVPLEGEDPPKTSWSSDELAFFRRCLDRV
                     GRASATIDGPPQVACIAARYLAPGGGSWTASFAEIEHGLEFVSRQACADRIVPILAGG
                     IGPDEGGLYAPYDGAGVFMLSSV"
BASE COUNT          698 a         1377 c         1347 g          574 t
ORIGIN      
        1 ggatgatttc gcccttgttg atgacgccga tgcgatcggc catctcctcg gcttcctcga
       61 tgtaatgcgt ggtcaggatg atggtgacgc cggaagcctg cagggcgcgc accacttccc
      121 acatcccctt gcgcaattcg acgtcgacgc ccgcggtcgg ctcgtcgaga aacaggatct
      181 gcggctcgtg cgacagcgcc ttcgcgatca tcacgcggcg cttcatgccg ccggacagcg
      241 tgatgatctt gttgtccttc ttgtcccaga gcgagagatc cttcagcacc ttctcgatat
      301 gcgccgggtt tttcggcttg ccgaacaggc cgcggctgaa gctcaccgtc gaccacaccg
      361 attcgaaggc gtcggtgctc agctcctggg gcaccaggcc gatcatggtt cgcgcggcgc
      421 ggtagtcggc gacgatgtcg tggccggcga cgctgaccga gcccgagctg gcattgacga
      481 tgccgcagac gatgctgatc agcgtcgtct tgccggcgcc gttcgggccg agcagcgcga
      541 agatctcgcc gcggccgatc gcgagatcga tgttccgcag ggccgtgaag cccgacgcat
      601 aggttttcga cagcttcgag acgcgaagga tgtcctgcat cggcccgagg ataacggcgg
      661 catcgccaag gtcggcaaaa cgtgcatttt ccgcgctgcc cgttccaccg aaacggcgaa
      721 cgctggccca atcgacggct tcgcaacccg cgccaacgct gcccatgccc gccgccgacc
      781 tgaccgaaga acaagcccgt acctacatgc acaagctgct ggccgcgatg agccaggccg
      841 gcggctccga cctcttcatc gccgccgact tcccgccgag catgaaggcc aacggcaaca
      901 tgcagccgct cagccagcaa aagctcggcg ccgaggcgac gcggatgctg gcccatgcgc
      961 tgatgaacga ggcccagcgc gaggagttcg acaaggagat ggagtgcaac ttcgccatct
     1021 cgattcccgg cgtctcgcgc tttcgcgtca acgtccacat gcagcagcgc tgcgtcagca
     1081 tggtgatccg caccatcgcc tccgagatcc cgagcttcga gaagctgaag cttcccgaga
     1141 cgctcaagga ggtggtgatg accaagcgcg gcctggtgct cgtggtgggc gccaccggct
     1201 cgggcaagtc gacgacgctg gcggcgatga tcgaccatcg caaccgcgcc tcggccggcc
     1261 acatcatcac cgtggaagac ccggtcgagt acgtgcacgt gtcgcagaag tcgctcgtca
     1321 cgcaccgcga gatcggcatc gacaccaaga gctggcatca cgcgctcaag aacaccttgc
     1381 gccaggcgcc cgacgtgatc ctgatcggcg agatccgcga cgccgagacg atggagcacg
     1441 ccatcgcctt cgccgagacc ggccacctct gcctgggcac gctgcacgcc aacagcgcca
     1501 gccagacgat cgagcggatc atcaatttct ttcccgacga gcggcgccag cagctgctga
     1561 tggacctctc ggccaacctg cgcgcgatcg tctcgcagcg cctggtgcgg cgcgaggacg
     1621 gcaaggggcg ctcggcggcg atcgagatcc tcctcaacac gcccagcatc gccgagaaaa
     1681 tcttccagag cgccttccac gagatcaagg gcctgatggc gaagtcgcgc gagctcggca
     1741 tgtgcacctt cgacgccgcc ctgttcgatc tctacaacga tggcgccatc agcttcgacg
     1801 aggcgatacg caacgccgac tcggccaacg agctgcgcct caacatcaag ctcaagagca
     1861 agcgcggcga gcccaagcag tcggcgttcg gcggcctgtc gatcgacccg atgacgcaac
     1921 ccgccgacct cgaggagcgt gcccaccagg agatgctgaa gaagcaggaa tcgcggcggc
     1981 gcttcgagga agagcagctg cgccagttgc gcgagaaaaa gcgcctcgcc gaagaagcgg
     2041 cgatggcgac gaaggggccg gtcctgccgg ggtcgatgtc gaactgacgg tcggcgacgc
     2101 ggcgccggcg acgcaatgcc ccgggcctca ggaggcgaca gcgcgctcgc cgatccagtt
     2161 cgacgtcttg tccagcatcg cctgcagcac ctcggcgtcg gtgcggccgc tgcgggcgcg
     2221 cacatggaac gcgtggtcgg catcgtcgaa ccaggccagt gtcgcccgcc cgcccagttg
     2281 gccgatgacg ggcagaagga gttccggcgc ggcaagctcg tcgcgcgtgc cctgcaggaa
     2341 gagcatcggc aatcccacct tgtccaggtg ggccgcccgc tcgttcgaag gcttgccggc
     2401 cggatggagc ggaaagccga cgaagacgag cccgagcacg ccggggagcg gcgccagcgc
     2461 ctgcgcctga gatgtcatcc gcccgccgaa cgacttgccg cctgcgaaga ggggcagtcc
     2521 ggcacagtgc tcggcggccg tcctgaccgc gccgcggacc gtcgcgtggg cgagcgacgg
     2581 cgggtcggga cgccgggagc ccctggccat gaagggaaag tcgaagcgca gcgtggcgat
     2641 gtgccgcgcc gccaggccgg cggcgaacgc tgccatgaag ccatgccgca tcccggcgcc
     2701 ggcgccgtgc gcgaagacat agcaggcacg cgccgacggc ggccgcagca gcagcgcggg
     2761 cacggcgcca tgcgcctcga caggcaccga aagggcgatc gcgccggcgt ccgggccggc
     2821 cgtcacggga gcatcggtgg ccatgaccgc attgtcccgg agcgagcggc aggctcgccg
     2881 cagcggcctt tcctcgccgc cttcatgcgg gctctctcga ccaggaaaac gacaccgcgc
     2941 cgatgccgcg acgggcttta cgccaacgcg tgccgccccg atgatggcgg tcggatgcgc
     3001 ccgacacgcg cccggccggc tcgccgacac agtctggcgg acgcgctcga tatcgttgcc
     3061 ctgggccgcc ctggagtgaa cgcatgatga ccccgacccc ggaaattctc gacttcgacg
     3121 cctggtgcga gcgctgcgcg cgtcgcctcg gcgagatcta cgaccagctg acgatcgccg
     3181 aggcgcggcg cctggcccgc gacgtctacg ccttcgagcg gacgcgggcg atggatcctt
     3241 cgcgggcggc agagttcgtc gccgtcgaga tgacgcggcc cgaccggggg ccgttcgagc
     3301 ggcgcgcggt ggcgcgctga gcgtgtcgcg ccgccggcgc tcgacatccg ccgcctttca
     3361 gacgctgctc agcatgaaca caccggctcc gtcgtacggg gcgtagaggc cgccctcgtc
     3421 ggggccgatg ccgccggcca ggatcggcac gatgcgatcg gcgcaggcct ggcgcgagac
     3481 gaattcgagc ccatgctcga tctcggcgaa cgacgccgtc cagctgccgc cgcccggcgc
     3541 gagatagcgg gcggcgatgc aggccacctg tggcggtccg tcgatcgtgg ccgatgccct
     3601 gccgacgcgg tcgaggcatc ggcggaaaaa ggcgagctcg tcgctcgacc agctcgtctt
     3661 cggaggatct tcgccttcga gcggcacgta gacgaaggtc tgctggaact tcgcctcgaa
     3721 gcgggagaag tcggggacca ccacgaccat cggccgggcg aagccgacga agcgccgggt
     3781 gaattcggcc agcccggcgc ggcgctcgaa gctctgccac cgctcgctgc gctggcgaat
     3841 gaaaagcacc ttcttcgttt cgatgccggt catgacggcc acggtgggcg gaggcacggg
     3901 atatttcatg gacgcaacct cgcttcgacg caacctggac gcgcgacgga cggctcagcg
     3961 cggcacggtg tcttggacgc gggcccgcgc gagatc
//
LOCUS       DASRWS010000259         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_972025, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000259 DASRWS010000000
VERSION     DASRWS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972025"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="gnd"
                     /locus_tag="VFF72_04725"
     CDS             complement(<1..938)
                     /gene="gnd"
                     /locus_tag="VFF72_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZV92497.1"
                     /translation="MQLGMIGLGRMGASMVRRLMKRGHECVVFDMQAKAVDAMAAEGA
                     RGAKTLQEFVAAMARPRAIWLMVPAGVVDKELDELVAHLDRDDIVIDGGNSYYRDDIR
                     RAGKLAESGLHYVDVGTSGGIAGQDRGYCLMIGGEDSVVARLKPIFEALAPGVDAAPR
                     TRGRSGDVSAAEQGFLHCGPHGAGHFVKMVHNGIEYGVMAAYAEGLNILRHANIGKRE
                     QTHDAETTPLRHPEYYQYEMKLPEIAEVWRRGSVIGSWLLDLTANALLADPELAAYGG
                     RVSDSGEGRWTIAAAIDEGVPAPVLATALFDRFISQG"
     gene            complement(952..>3051)
                     /locus_tag="VFF72_04730"
     CDS             complement(952..>3051)
                     /locus_tag="VFF72_04730"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HZV92498.1"
                     /translation="WLELFVAIDAPVKFSVLRLSNRSARQRTLSVTGYVEWVLGDERS
                     KTLMHVVSERDDSGALFARNDYATDFAGRVAFLDIDVDAPGASAGDPAGAVTFCADRG
                     AFFGASGSLAAPDALVEPALCGLVGAGLDPCAAMRVSFALAPGESRDVILRLGAGHSR
                     DEARALVRRWRGAAAAAEALAAARAWWRKTLGTLEVRTPDPAVDALANGWLLYQVIAS
                     RLWGRTAFYQSSGAYGFRDQLQDVMSLVHVAPSLVREHLLRAAGRQFVEGDVQHWWHP
                     PTGKGIRSRCSDDYLWLPFVACRYVEATGDASVMDVDVPFLQGAGLKDGEASDYALPQ
                     ISSEAASLYAHCVRAIQHGLRYGEHGLPLMGGGDWNDGMNLVGIEGKGESVWLAFFLV
                     AVLRRFEPVARGRGDGAFADRCAEESKALSERIEQAAWDGAWYQRAWFDDGTALGVDG
                     RSECRIDSIAQSWSVLSAAAPRERARTAMASLHQQLVHADSRVIQLLDPPFDTSTPSP
                     GYIQGYVPGVRENGGQYTHAAVWAALAFATLGDSRRAWELFGLLDPLGHGDSAERIAV
                     YKAEPYVIAGDVYAFAPHAGRGGWTWYTGSAGWMSQLVVEALLGFERRGNQLRLRPLL
                     PGHWPSFEMRYRFGESSYGIVCRAAAQGEGERLVVDGRDVDDGVITLVDDGGRHAVEV
                     TVRRDNGSEPVQTRHPH"
BASE COUNT          450 a         1072 c         1089 g          440 t
ORIGIN      
        1 ccttgcgaga tgaaccggtc gaacagcgcg gtcgcgagca cgggcgcggg cacgccttcg
       61 tcgatggcgg ccgcgatcgt ccagcggcct tcgccggaat cggagacgcg accgccgtag
      121 gcggcgagct cggggtcggc aagcagcgcg ttggcggtga ggtcgagaag ccacgagccg
      181 atcacgctgc cgcggcgcca gacctcggcg atctcgggca gcttcatttc gtactggtag
      241 tactcggggt ggcgcagcgg cgtcgtctcg gcgtcgtgcg tctgctcgcg cttgccgatg
      301 ttggcgtggc gcaggatgtt gagcccttcg gcgtacgctg ccatgacgcc gtactcgatg
      361 ccgttgtgca ccatcttcac gaagtggccg gcaccatgcg gaccgcaatg caggaagccc
      421 tgctcggccg ccgacacgtc gccgctgcgg ccgcgggtgc gcggcgccgc gtcgacgccc
      481 ggggccagcg cctcgaagat cggcttcagc cgggcgacga cgctgtcctc gccgccgatc
      541 atcaggcagt agccgcggtc ctggccggcg atgccgccgc tggtgccgac gtcgacgtag
      601 tgcagtcctg actcggccag cttcccggca cggcggatgt cgtcgcggta gtacgagttg
      661 ccgccgtcga tgacgatgtc gtcgcggtcg agatgggcga cgagttcgtc gagctccttg
      721 tcgacgacgc ccgccggcac catcagccag atcgcccgcg gcctggccat cgcggcgacg
      781 aattcctgca gcgtcttcgc gcccctggcg ccttccgcgg ccatcgcgtc gaccgccttg
      841 gcctgcatgt cgaagacgac gcactcgtgg ccccgcttca tgaggcggcg caccatgctc
      901 gcgcccattc gcccgaggcc gatcattccg agctgcatgc gtgttctcct gtcagtgagg
      961 gtgacgagtc tggaccggtt cgctgccgtt gtcgcggcgc accgtcacct cgaccgcatg
     1021 acgcccgccg tcgtcgacca gcgtgatcac gccgtcgtcg acgtcacggc cgtcgacgac
     1081 gaggcgttct ccctcgcctt gcgccgcggc ccggcagacg atgccgtagc tcgactcgcc
     1141 gaagcgatag cgcatctcga acgaaggcca gtgccctggt aggaggggac gcagccggag
     1201 ttggttcccg cgtcgctcga agccgagcaa tgcctcgacg acgagctgcg acatccagcc
     1261 cgccgagccg gtgtaccagg tccagccgcc gcggccggcg tgcggcgcga aggcgtagac
     1321 gtcgccggcg atcacgtagg gctcggcctt gtagacggcg atgcgctcgg cgctgtcgcc
     1381 gtggcccagc gggtcgagca ggccgaacag ctcccaggct cgtcggctgt cgccgagcgt
     1441 cgcgaaggcc agcgccgccc agaccgcggc gtgcgtgtac tggccgccgt tttctcgaac
     1501 gcccggcacg tagccctgga tgtagccggg cgacggcgtc gacgtgtcga aaggcggatc
     1561 gagcagctgg atgacacgcg aatcggcatg cacgagttgc tggtgcaggg acgccatcgc
     1621 ggtgcgggcg cgctcgcgcg gcgcggccgc cgacagcacc gaccagctct gcgcgatcga
     1681 gtcgatgcgg cattcgctgc ggccgtcgac gccgagcgcc gtgccgtcgt cgaaccatgc
     1741 gcgctggtac caggcaccgt cccacgcggc ttgctcgatc cgctccgaca gcgccttgga
     1801 ttcctcggcg cagcgatcgg cgaaggcgcc gtcgccgcgg ccgcgcgcga ccggctcgaa
     1861 acgacgcagc acggcgacga ggaagaaggc cagccacacg ctttcgccct tgccttcgat
     1921 gccgacgagg ttcatgccgt cgttccagtc gccgccgccc atcagcggca agccgtgctc
     1981 gccgtagcgc agcccgtgct ggatcgcgcg gacgcagtgc gcgtagagac tcgcggcctc
     2041 ggacgagatc tgcggcagag cgtagtcgct ggcctcgccg tccttcaggc cggcgccttg
     2101 cagaaacggc acgtcgacat ccatgaccga ggcgtcgccg gtcgcctcga cgtagcggca
     2161 ggcgacgaac ggcagccaca ggtaatcgtc ggaacagcgg ctgcggatgc ccttgccggt
     2221 cggcggatgc caccagtgct ggacgtcgcc ctcgacgaac tgccgtccgg cggcgcgcag
     2281 caggtgttcg cggaccagcg acggtgcgac gtggaccagc gacatcacgt cctgcaactg
     2341 gtcgcgaaag ccgtaggcgc cgctcgactg gtagaacgcc gtgcgtcccc agaggcgcga
     2401 ggcgatcacc tgatagagca gccagccgtt ggcgagcgcg tcgaccgccg gatcgggcgt
     2461 gcggacctcg agcgtgccga gcgtctttcg ccaccaggcg cgggccgcgg ccagcgcttc
     2521 ggcagccgcc gcggcgccgc gccagcgacg cacgagggcc cgcgcttcgt cgcggctgtg
     2581 ccccgcaccg aggcgcaaga tgacgtcgcg cgattcgccc ggcgccagtg cgaaactgac
     2641 tcgcatcgcc gcgcacgggt cgaggccggc gccgacgagg ccgcataacg ccggctcgac
     2701 gagcgcgtcg ggcgcggcga gcgagccgct cgcgccgaag aaggcaccgc gatcggcgca
     2761 gaaggtgacc gcgccggcgg ggtcgccggc cgacgcgccg ggcgcatcga cgtcgatgtc
     2821 gaggaaggcg acgcggcccg cgaagtcggt cgcgtagtcg ttgcgtgcga agagggcgcc
     2881 gctgtcgtcg cgctcgctga cgacgtgcat cagcgtcttg ctgcgctcgt cgccgaggac
     2941 ccattcgacg tagccggtga cgctcagcgt gcgttgccgc gccgaacgat tcgagaggcg
     3001 cagcaccgag aacttgaccg gcgcgtcgat ggcgacgaag agctcgagcc a
//
LOCUS       DASRWS010000260         5733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1257170, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000260 DASRWS010000000
VERSION     DASRWS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257170"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFF72_04735"
     CDS             complement(<1..908)
                     /locus_tag="VFF72_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZV92499.1"
                     /translation="MSLLHEIGVGFAVALLAPQLLAALCGAMLGTLLGALDVVSAPAA
                     IALLLPAMARLDATESIVLLAAVWYGSRYGAAVAAILLKAPADSVAAGTDGGQMARQG
                     RGHAALASAWLGTCVAGGIGALVLAVLALPLAEIAFRFGPAEYFSLIVLALVAAVAFS
                     PGSFVKGVSMLLLGLLLAQAGTDAASSSVRFAFGLEPLAGGIGIVALAVGLLAVGEMI
                     ARAGSVVVARDVIAGALHGERPRLDELREAWPSILRGSALGAMLGLLPGRGASFAASA
                     AYAIERRVAVEPRVPFGRGAVQGVAGP"
     gene            complement(943..1458)
                     /locus_tag="VFF72_04740"
     CDS             complement(943..1458)
                     /locus_tag="VFF72_04740"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZV92500.1"
                     /translation="MRIKSERDFWSGLAFVVVGVAFAVASTHHAMGPSCTAATPCADA
                     PLARLAQISARPGPGLLPLGLGVLLAISGALVLFKSMTLESEGGDLVGSVAWRPLAAV
                     VGALVAFAVLIEPLGWAAAVVMLVAIAGVAMPGWRWQRALVEIVGLSLLAWLVLFWIL
                     KLPVPAWPVHG"
     gene            complement(1550..3448)
                     /locus_tag="VFF72_04745"
     CDS             complement(1550..3448)
                     /locus_tag="VFF72_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZV92501.1"
                     /translation="MKTPIKIVRPEPPASPVAAGVAELAHERSISPSRAAGGFANTDL
                     SLGVVTSGKLTWRTVLAWLRDDKVLSAEDVERTARRFAGGNSAQHPLVRLGSAGLTRV
                     GDGKVLDVETLTEWLAKHSKLPYMRIDPLRVDVGRVADVMSINYAERRHALPLTFGAN
                     EVTIATCEPLDVAWVPEIESHTKRTVRLVIVNPVEIARYTTEFYALSRSVRNATKMGE
                     SAALANFEQLVELGKSSKQLDANDSGVIQVVDWLWQYAFDQRASDIHLEPRRERSVIR
                     FRIDGVMHTVYQLPPGVMSAMVARVKLLGRMDVVEKRRPLDGRIKVRNPAGDEVEMRL
                     STIPTAFGEKLVMRIFDPDTTVKSLESLGFGNHDGRRWEELVSKPHGIILVTGPTGSG
                     KTTTLYSTLRRLATDEVNVCTIEDPIEMIQPAFNQTQVQPQIDLNFAEGLRSLMRQDP
                     DIIMVGEIRDLQTADMAIQASLTGHLVFSTLHTNDSASAITRLNDLGVAPYLIGSTLI
                     GVLAQRLVRTLCPTCKVPDEEVTRETLHEVVKPWRLNGGVRPYKPVGCLECRMTGYRG
                     RAGLFELLTVSDAARSAIQPVCDETKLRAQALQDGLRPLRLAGAMKVAEGVTTLDEVL
                     RATPAWEQ"
     gene            complement(3445..4281)
                     /locus_tag="VFF72_04750"
     CDS             complement(3445..4281)
                     /locus_tag="VFF72_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HZV92502.1"
                     /translation="MSRAAVPSNAPVPGSAGAELPEVTISESDGVRFLHLGTPWIQGA
                     MRIDDPLDIELEYVQRMMAWLLCREDAAAPRGHAVQLGLGAGAITRFCLRRLKMRTTA
                     VELNRRVIDACRHWFRLPEESARFAMLEMDAGAFVADPRRRETIDVLCVDLYDAEAAG
                     PVLDSAEFYAACHGALCDGGVMTVNLFGRAATFERSVERIVAAFGVDGVRSLKPTREG
                     NSVVIAMKHVTFPGRDELARRAENIETRWKLPARKWLRILRANPDPSPAATLPLPALP
                     PA"
     gene            complement(4292..5113)
                     /locus_tag="VFF72_04755"
     CDS             complement(4292..5113)
                     /locus_tag="VFF72_04755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZV92503.1"
                     /translation="MWIDTHCHLDAAEFGGDRAAVVVAARAAGVGMIVLPAVDAGNFD
                     TVRELSKRHGLAYAIGIHPMCTGRAGDADLARLRAALQVARGDPRLVAIGEIGLDHFV
                     AGLDPARQGDFFAAQLDLAVEFDLPVLLHVRRAIDPVLKQLRSRRVPGGIAHAFNGSE
                     QQAEVFSGLGFRLGFGGTLTFERALRIRRVARTVPLEAIVMETDAPDIAPQWLYRTAE
                     ARAAGASMRNEPAELPRIGRELAALRGIETAAVEAATTANALASLPRLAALMSAP"
     gene            5283..>5733
                     /locus_tag="VFF72_04760"
     CDS             5283..>5733
                     /locus_tag="VFF72_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HZV92504.1"
                     /translation="MKKVPLYSRSQRPVSQPPAGETSGTHDADLTAPPASRKDRLGAF
                     VVRHERRLWALALVSLAAAFWWLNPTLVPKAPLTMAQIDAAMRQSIEDKPLIAPESRA
                     YDAILPSVVRVVGLMTEGDDGSEEKEHGNVERGIGSGVVIIDNGTILT"
BASE COUNT          918 a         2015 c         2037 g          763 t
ORIGIN      
        1 ggaccggcga cgccctggac cgcgccccgc ccgaatggca cgcgcggttc gaccgcgacc
       61 cgccgctcga tcgcgtaggc ggccgacgcg gcaaaggatg cgccgcggcc gggcagcagt
      121 cccagcatgg cgccgagcgc actgcctcgc aggatcgacg gccaggcctc gcgaagttcg
      181 tcgaggcgcg gccgctcgcc gtgcagcgca ccggcgatga catctcgagc gacaacaacc
      241 gagccggcgc gggcaatcat ctcgccgaca gcgaggagcc cgaccgccag cgcgacgatc
      301 ccgatgcccc cggccagcgg ctccaggccg aaggcgaatc gcacgctgga cgacgccgcg
      361 tcggtgcccg cctgcgccag caagaggccc agcagcagca tcgacacgcc cttgacgaac
      421 gagccgggcg agaacgcgac agcggccacc agcgccagga cgatcaggga aaagtattcg
      481 gcgggaccga agcgaaaggc gatttcggcc agcggcagtg ccagcacggc gaggacgagc
      541 gcgccgatgc cgccggcgac gcaggtgccg agccatgccg aggccagggc cgcgtggccg
      601 cggccctgtc gtgccatctg gccgccatcg gtgccggcag ccacggaatc ggccggcgcc
      661 ttcagcagga tcgcggcgac ggccgcgcca tagcgcgagc cgtaccagac cgccgccaac
      721 aggacgatcg actcggtggc atcgaggcgc gccatggccg gcagcagcaa ggcgatcgcc
      781 gcgggcgcac tgaccacatc gagcgccccc agcagcgtgc ccagcatcgc gccgcacagc
      841 gccgccagca attgaggcgc cagcagcgcg accgcgaagc cgacgccgat ctcgtgcagc
      901 aggctcattc gggcccggcc gcgaagtgac cgccgaagac gttcaaccgt gcaccggcca
      961 cgcgggcacg ggaagcttca ggatccagaa cagcaccagc caggccagga gactcaggcc
     1021 gacgatctcg acgagcgcgc gctgccagcg ccagccgggc atcgcgacgc cggcgatggc
     1081 gacgagcatc acgacggcag ccgcccagcc gagcggctcg atcaggacgg cgaaggcgac
     1141 cagcgcgccg acgacggcgg cgagcggccg ccaggcgacg gagccgacca ggtcgccgcc
     1201 ttcgctttcg agcgtcatcg acttgaagag caccagggcg ccggagatcg ccagcagcac
     1261 gccgagcccg agcggaagca gccccggacc gggccgggcc gaaatctgcg ccaggcgcgc
     1321 cagcggcgcg tcggcgcatg gtgtcgccgc ggtgcaggac ggccccatcg catggtgggt
     1381 cgacgccacg gcgaaggcga cgccgacgac gacgaaagcc aggcccgacc agaagtcgcg
     1441 ctcgctcttg atcctcatgc tggcctctcc tggccaggtg cgccgctcgc gtcgtcaggc
     1501 aggcacatcg aacgacgggc gccgcgatcg cgccgtgacc ggactcgcct cactgctccc
     1561 aggcaggcgt ggcgcgcagc acttcgtcga gcgtcgtcac gccctcggcg accttcatcg
     1621 cgccggcgag gcgcagcggt cgcagcccat cctgcaaggc ctgggcgcgc agcttcgtct
     1681 cgtcgcagac cggctggatc gcgctgcgtg ccgcatcgct caccgtcagc agctcgaaga
     1741 ggccggcgcg gccgcggtag cccgtcatgc ggcactcgag gcaaccgacc ggcttgtacg
     1801 gccgcacgcc gccattcagt cgccacggct tgacgacttc gtgcagcgtc tcgcgcgtca
     1861 cctcctcgtc gggcaccttg caggttgggc agagcgtgcg aacgaggcgc tgcgccagca
     1921 cgccgatcag cgtcgagccg atgagatacg gcgcgacgcc gaggtcgttg agacgcgtga
     1981 tcgccgaggc cgagtcgttg gtgtgcagcg tcgagaagac gaggtggccg gtgagcgacg
     2041 cctggatcgc catgtcggcg gtctgcaggt cgcggatctc gccgaccatg atgatgtccg
     2101 ggtcctggcg catcagcgag cgcaggcctt cggcgaagtt gaggtcgatc tgcggctgca
     2161 cctgcgtctg gttgaacgcc ggctggatca tctcgatcgg gtcttcgatc gtgcagacgt
     2221 tgacctcgtc ggtggccagg cggcgcagcg tcgagtagag cgtcgtcgtc ttgcccgagc
     2281 cggtcgggcc ggtgacgagg atgatgccgt gcggcttgga gaccagctct tcccagcgcc
     2341 ggccgtcgtg gttgccgaag cccaggctct cgagcgactt gacggtcgtg tcgggatcga
     2401 agatccgcat caccagcttt tcgccgaagg ccgtcgggat cgtcgacagg cgcatctcga
     2461 cctcgtcgcc ggcggggttg cggaccttga tgcggccgtc gagcggacgg cgcttctcga
     2521 ccacgtccat gcggccgagg agcttcacgc gtgccaccat cgcgctcatc acgcccggcg
     2581 gcagctggta gacggtgtgc atcacgccgt cgatgcggaa gcggatgacg ctgcgctcgc
     2641 ggcgcggctc gaggtggatg tcggaggcgc gctggtcgaa ggcgtattgc cagagccagt
     2701 cgacgacctg gatgacgccc gaatcgttgg cgtcgagctg cttcgagctc ttgccgagct
     2761 cgaccagctg ctcgaagttg gcgagcgccg ccgactcgcc catcttcgtg gcgttgcgca
     2821 ccgagcgcga gagggcatag aactcggtcg tgtagcgggc gatctcgacc gggttgacga
     2881 tcaccaggcg caccgtgcgc ttggtgtgcg actcgatctc cggcacccag gcgacgtcga
     2941 gcggctcgca ggtggcgatc gtcacctcgt tggcgccgaa ggtgagcggc aacgcgtgcc
     3001 ggcgctcggc gtagttgatc gacatgacgt cggcgacgcg gccgacgtcg acgcgcagcg
     3061 gatcgatccg catgtagggc agcttgctgt gcttggcgag ccactccgtc agcgtctcga
     3121 catcgagcac cttgccgtcg ccgacccgcg tcaggcccgc gctgccgagg cgcacgagcg
     3181 gatgctgggc gctgttgccg ccggcgaagc gccgcgccgt ccgctcgacg tcttcggcac
     3241 tgagcacctt gtcgtcgcgc agccacgcga gaaccgtgcg ccaggtgagc ttgccgctcg
     3301 tgacgacgcc gagcgacagg tcggtgttcg caaacccgcc ggccgcacgc gacggcgaga
     3361 tcgatcgctc gtgcgccagc tcggcgacgc cggcggcgac cggcgatgcc ggcggttcgg
     3421 ggcgcacgat cttgatcggc gttttcatgc gggaggaagg gcgggaagag gcagcgtggc
     3481 ggcaggcgac ggatcagggt tcgctcgcag gatgcgcaac cacttgcggg ccggcagctt
     3541 ccagcgagtt tcgatgtttt cggcacgccg ggcaagctcg tcccggccgg ggaacgtgac
     3601 atgcttcatc gcgatcacca cggaattgcc ttcgcgcgtc ggtttcaggc tgcgcacgcc
     3661 gtcgacgccg aaagccgcga cgatgcgctc gacgctgcgc tcgaaggtcg cggcccgccc
     3721 gaacaggttg accgtcatca cgccgccatc gcacagcgcg ccgtggcagg ccgcatagaa
     3781 ctcggcgctg tcgaggaccg ggcccgcggc ctcggcgtcg tagaggtcga cgcacagcac
     3841 atcgatcgtc tcccgtctgc gcggatcggc gacgaaggcg ccggcatcca tttcaagcat
     3901 cgcgaatcga gccgattcct cgggcaggcg aaaccagtgc cggcacgcgt cgatgacgcg
     3961 tcgattgagc tcgaccgccg tcgttcgcat cttcagccgg cgcaggcaaa agcgggtgat
     4021 ggcgcccgcg ccgaggccga gctggacggc gtggccgcgg ggcgccgcag cgtcttcgcg
     4081 acagagcagc cacgccatca tccgctgcac gtactcgagc tcgatgtcga gaggatcgtc
     4141 gatccgcatc gcaccctgga tccacggcgt gcccaggtgc aggaagcgca cgccgtcgga
     4201 ctccgagatc gtcacctcgg ggagctcggc ccccgcactc ccgggcaccg gggcattcga
     4261 cgggacggcc gcgcgactca cgacgacgca ctcacggcgc ggacatcagc gcggcgagcc
     4321 gaggcagcga cgccagcgca ttggccgtcg tcgcggcttc gaccgcagcc gtctcgatgc
     4381 cgcgcagcgc cgcgagctct cggccgatcc tcggcaactc ggccggctcg ttgcgcatcg
     4441 acgcgccggc cgcccgtgcc tcggccgtgc gatagagcca ctgcggcgcg atgtcgggcg
     4501 cgtcggtttc catgacgatc gcctccagcg gcacggtccg ggcgacgcgc cggatccgca
     4561 acgcgcgctc gaaggtcagc gtgccgccga atccgagacg aaagccgagc ccgctgaaca
     4621 cctcggcctg ctgctcgctg ccgttgaacg cgtgggcgat gccgccgggc acgcgccgtg
     4681 agcgcaactg cttgagcacc ggatcgatcg cgcgacgcac gtgcagcagc acgggaagat
     4741 cgaactcgac ggcgaggtcg agctgtgccg cgaagaaatc gccctggcga gccgggtcga
     4801 ggccggcgac gaaatgatcg aggccgatct cgccgatcgc gaccagtcgc gggtcgccgc
     4861 gcgcgacctg gagggcggcg cgaagccggg ccaggtcggc gtccccggcg cggccggtgc
     4921 acatcgggtg gatgccgatc gcatacgcca gcccatgccg cttcgagagc tcgcgcacgg
     4981 tgtcgaaatt gcccgcatcg accgccggca gcacgatcat gccgaccccg gcggcgcggg
     5041 cggcgacgac gacggcggcg cggtcgccgc cgaactcggc cgcgtcgaga tggcaatgcg
     5101 tgtcgatcca cacggtaggc gatgatgcct cggccgcgac agcgcccttt gccggacgcg
     5161 ccgggcccgg ccgtgctacc gtactgcgca taggcgtcgg cttcgttgcg cgggccgctc
     5221 gcgcggtgcc tcgaagcgtg acgcgcgcgc cctgtccgcg gatgcgtttg ccggatccga
     5281 cgatgaagaa agttccgctt tacagtcgat cgcagcgccc cgtttcgcag ccgcccgcgg
     5341 gcgagacgtc cggcacccac gacgcagatc tgacggcgcc gccggcgtcg cgaaaggacc
     5401 gcctcggcgc gttcgtcgtt cgccacgagc ggcgcctctg ggcgctggcc ctcgtcagcc
     5461 tcgcggcggc gttctggtgg ctgaatccga cgctggtgcc gaaagcgccg ctcaccatgg
     5521 cgcagatcga cgcggcgatg cgccagtcga tcgaggacaa gccgctgatc gcccccgagt
     5581 cgcgcgccta cgacgcgatc ctgccctcgg tggtgcgcgt cgtcgggctg atgaccgaag
     5641 gcgacgacgg cagcgaggag aaggagcacg gcaacgtcga gcgcggcatc ggcagcggcg
     5701 tcgtcatcat cgacaacggc accatcctga cga
//
LOCUS       DASRWS010000261         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1257792, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000261 DASRWS010000000
VERSION     DASRWS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257792"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFF72_04765"
     CDS             <1..1238
                     /locus_tag="VFF72_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZV92505.1"
                     /translation="TGEAHASASIGGRFGAPEYTGRVEASKLGVRNFLEGVDITDGRL
                     ALALHGAEARIEQFSARGGDGRVELTGDATFGSAPALSLSLRAEHFQLLGRVDRRIVV
                     SGNATVRIDASAFDVSGKLGVDEGLVDFTRSDAPTLGDDVEVVRRPAAPAATAAADRT
                     QAPPAAPPTNATATARRLKLDVRVSMGEKLRIRGRGLDARLTGDLHLTSPEGRLAVDG
                     TLRVVDGTYQAYGQKLGIDRGVLSFVGPVDNPRLDIEATRPNLDVRVGVTVTGTALNP
                     RIRLFSEPDMSDVDKLSWLVLGRASAGVGADDMALLQSAALALLSGEGPGVTDRLTHA
                     FGLDTFGVRQQSEGDVKETIVSLGKQISKRWYIGYERGLNATSGSWQLIYRIARRFTV
                     RAQAGDDNSIYLIWTLRWN"
     gene            complement(1329..1556)
                     /locus_tag="VFF72_04770"
     CDS             complement(1329..1556)
                     /locus_tag="VFF72_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92506.1"
                     /translation="MAILEGSHPQAGGRQVAYRAEYEVVGKTIHFKARFDNGDTHEGE
                     FDFDASRLDAAAAVDAFMHDHIEKADRDVAP"
     gene            1656..1727
                     /locus_tag="VFF72_04775"
     tRNA            1656..1727
                     /locus_tag="VFF72_04775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:cct)
     gene            1904..3319
                     /locus_tag="VFF72_04780"
     CDS             1904..3319
                     /locus_tag="VFF72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZV92507.1"
                     /translation="MGLTDTKLRNLKPPERPKRVFDGDGLYIELRPNGGKWWRFKYRF
                     DGKEKSLSFGTYPEVPLALARARRTEARMLVAAGTDPSAVRKQAKQAKQRAAVNTLEA
                     VSRAWLARRAPAWTKGTLEAITASLENHVFPSLGARPIAEIPARDIRVVVQAIDDQGA
                     GETAGRVFQRLRSIYRYALSEELVEADPTHALKPSEIFRPRSVSHRLALSATDMPAFF
                     RKLDVYEGDPSTKAALELLILTAVRPGELRGARWQEMDLSRSLWRIPANRMKMATEHL
                     VPLSTQALAVLEGMKALSGGQELVFPSPFYPGKPLSDGTLNSALTRLGYRGVATAHGF
                     RTLFSTCANEAGWNSDVIEKQLSHEERDEVRSAYNRAQWLPERVKLMQWWANHLQTLR
                     TVSTPSPGSQNKGSRRPGGRAGTRVRKRLGASTGVALARTGISGVPTPAPSLQSAVAD
                     PIEVLRALVAAFDRVSSRDQR"
BASE COUNT          712 a         1312 c         1267 g          640 t
ORIGIN      
        1 tgaccggcga agcgcacgcc agcgcgagca tcggcggacg cttcggtgcg cccgagtaca
       61 ccggccgcgt cgaggcgtcg aagctcggcg tgcgcaattt cctcgaaggc gtcgacatca
      121 ccgatggccg actggcgctc gcgctgcacg gcgcggaggc gcgcatcgag cagttctcgg
      181 cacggggcgg cgacggacgc gtcgagctga cgggcgatgc gaccttcgga tcggccccgg
      241 cgctttcgct ctcgctacgc gccgagcact tccagctgct cggccgcgtc gaccgccgca
      301 tcgtggtcag cggcaacgcg acggttcgca tagacgccag cgcattcgac gtctccggaa
      361 agctcggcgt cgacgagggc ctcgtcgact tcacgcgctc cgacgcaccc acgctcggcg
      421 atgacgtcga ggtcgtgcgc cgaccggcgg cgcccgccgc gaccgcagcc gcggatcgca
      481 cgcaagcgcc gcccgcggcg ccgccgacga acgccacggc cacggcacga cggctcaagc
      541 tcgatgtgcg cgtctcgatg ggggaaaagc tgcgcatccg cggccgcggc ctcgacgccc
      601 gtctgaccgg cgacctgcac ctcacctcgc ctgagggacg gctcgccgtc gacggcacgc
      661 tgcgcgtcgt cgacggtacc taccaggcgt acggacagaa gctcggcatc gaccgcggcg
      721 tcctcagctt cgtcggcccg gtcgacaacc cgcgcctcga catcgaggcg acccggccca
      781 atctcgacgt ccgcgtcggc gtcaccgtca ccggcaccgc gctcaatccg cgcatccggc
      841 tcttcagcga gcccgacatg agcgacgtcg acaagctgag ctggctggtg ctcggccgcg
      901 ccagcgccgg cgtcggcgcc gacgacatgg cgcttctgca aagcgcggcg ctggcgctgc
      961 tgtcgggcga agggccgggc gtgaccgacc ggctcaccca cgcattcggc ctcgacacat
     1021 tcggcgtgcg acagcaaagc gaaggcgacg tcaaggagac catcgtcagc ctcggcaagc
     1081 agatctcgaa gcgctggtac atcggctacg agcgcggcct caacgccacg agcgggagct
     1141 ggcagctgat ctaccggatc gcccgccgct tcacggtgcg cgcgcaggcc ggcgacgaca
     1201 attcgatcta cctgatctgg acgctgcgct ggaactagga gcggcaggcg cagaccggcg
     1261 aagcgcacgc atgagcgggc gactgcgtcg accgacgagg tcgcaacgcc cgaaagcgac
     1321 gaaccgcgtc aaggcgcgac gtcgcggtcg gccttctcga tgtggtcgtg catgaacgcg
     1381 tcgaccgcgg cggcggcatc gaggcgagag gcatcgaaat cgaattcgcc ctcgtgcgta
     1441 tcgccgttgt cgaagcgcgc cttgaaatgg atggtcttgc cgacgacttc gtactcggcg
     1501 cggtaggcga cctgccggcc gcccgcctgc ggatgggagc cttcgagaat tgccatggcc
     1561 tgctccgttt ctggcagcga cgatacgccc ggcccgcgcg cgtccggcga tcgaggtctt
     1621 acactgcccg ccgcggcgcg atctgccgcc gccagctcgc cgtagttcaa tggatagaac
     1681 ggccgcctcc taagcggcag atacaggttc gattcctgtc ggcgggactt ttgaatgatt
     1741 tcagggcgtc ccaagaaatc ccaaaaagtc ccctcagcgc gacgttttac cgggctagac
     1801 ggccgaatga tcgtcccggc acatacaaag cgagatcgtg acagccagca ctttttggag
     1861 tagattttag acggttaccc ccacggtttt tctgagttac cccatgggtc tgactgacac
     1921 gaagctgcgc aatctgaagc cgcccgagag gcccaagcgc gtcttcgatg gcgacggtct
     1981 gtacatcgag ctccggccga acggcggcaa gtggtggcgg ttcaagtacc ggttcgacgg
     2041 caaggaaaag tcgctgtcgt tcggcaccta tcccgaagta ccgctggcgc tggcacgtgc
     2101 ccgccggaca gaggctcgga tgctggtggc ggccggcacc gaccccagcg cggtgcgcaa
     2161 gcaagccaag caggcgaagc agcgagccgc agtcaatacg ctggaggctg tctcgcgcgc
     2221 ttggctggcg cgtcgcgctc cagcatggac gaaaggcacg ctcgaggcca tcacggcgtc
     2281 gctggagaac catgtcttcc cgtccctcgg tgctcgcccg atcgcagaga tcccggcgcg
     2341 cgacatcagg gtggtcgtgc aggcgatcga cgatcaaggc gctggagaga cagccggacg
     2401 ggtgttccag cgattgcgct cgatctaccg ctacgcgctc tctgaagaac tggtcgaggc
     2461 cgaccccacg catgcgctca agccgtcgga gatcttcagg ccgcgcagcg tcagccatcg
     2521 actcgcgctc tctgcgacgg acatgccggc gttctttcgc aagctcgatg tgtacgaggg
     2581 cgacccttcc acgaaggcgg cactcgagct gctgatcctc actgccgtcc gacctggcga
     2641 acttcgagga gcgcgatggc aagaaatgga tctttcccga tcgctctggc gaatcccagc
     2701 caaccggatg aagatggcaa ccgagcacct ggtgccgcta tcgacccaag cgctggccgt
     2761 tctggaaggc atgaaggcgc tgagcggcgg acaggagttg gtcttcccct ccccgttcta
     2821 tccgggaaag ccgctgagcg acggcactct gaacagcgcg ctgacccgcc tcggctaccg
     2881 gggcgttgct actgcgcatg gatttcggac gttgttttcc acgtgtgcca acgaagccgg
     2941 ctggaactcg gacgtgatcg agaagcagct gtcgcatgaa gagcgagacg aggttagaag
     3001 tgcctacaac cgagcgcagt ggctgcccga acgcgtcaag ctgatgcagt ggtgggcgaa
     3061 tcacttgcaa acgctccgca ccgtatcaac gccctcgccc gggtcacaaa acaaaggctc
     3121 ccgaaggccc ggaggtcgcg ccggcacgcg ggtgcggaaa cggctaggcg catccacggg
     3181 cgtcgccttg gcaagaacgg gaatcagtgg tgttccgaca ccggcacctt ccttgcagag
     3241 cgcagttgca gatccgattg aggtgttgcg ggcgttggta gccgcattcg accgagtttc
     3301 gagtcgcgat cagcgatagt ccagaagcga gcgtggctgt cgccgctgtt gaatacaggc
     3361 ttcgagggtg tctgccgcat cgctaggatc ggcaagcctt tgcggaacta tccgtgcacg
     3421 ccccccatcc cgcccctctt cctaaaagtg atgcccctct aggcacttgg ccgatcccac
     3481 agcgaacgca aagcgcgcga cggcttgtgc ccctaacgtc gacccttcct gcctttcgaa
     3541 acgaatgacg gcgggtcgtg ctctgaactc ccgctttcgg tcgcattgca gtcagccgag
     3601 cgtcagcgac cgaagggacc cgatggccgg cctcgaaggc actggtgaca ctgcgctggg
     3661 agccagcagg ttgcggtcac cgacatcttc cgaagtccaa ctagcttgat cgcgcgtgac
     3721 attcacacgc ccatctaccc gataggtccg gcttccgctg cctcgtcgtc gctaagtagt
     3781 ctgctgtaat cccctaacca atacattcag ggagacaggc atgtccggaa ggaacggcgg
     3841 cggtggagga ggcgggggtg gtgttcccga catccagcct tcctgcgacg cccttaagtt
     3901 cagcatcctt gtgtccagcc cgcaaccggc g
//
LOCUS       DASRWS010000262         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1258558, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000262 DASRWS010000000
VERSION     DASRWS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258558"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFF72_04785"
     CDS             <1..692
                     /locus_tag="VFF72_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZV92508.1"
                     /translation="AYGLVAETGFWPAALAWTERGGVLATAHVRGGGEYGEDWHLGGM
                     MKTKLNTVYDFIACGQYLVDQKYTTPRRLAAEGGSAGGIAVGRAMTMRPDLFGVILDH
                     VGLSDTLRFETEPNGPPNVVEFGSVKTEDGFHGLYAMSPYAHIVAGTAYPAVMYLTGA
                     NDPRVAPWNMMKMAARTQAATSSRKPVLLRIDDDAGHGIGSNRSQRERELADTWSFAL
                     WQMGDPAFQPK"
     gene            complement(775..1458)
                     /locus_tag="VFF72_04790"
     CDS             complement(775..1458)
                     /locus_tag="VFF72_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZV92509.1"
                     /translation="MKAPRLLAASVFLLASIGASATVTYTLGGGTGPYLALSGTTGST
                     CTGTAGADPCSLDGTFASISGGKIFTTGDPSFAALPAGTFGGNFLSAGPTPSNDEPAT
                     MTFASPISDIGFLWGSPDTYNLLTVETTMGNVSFTTGSLGFTVQDGDQSFSQYVWFHA
                     KDGAAITALVFDNNPATNAFEVANFSVSAVPEAQTYALMLFGLGALAMFARRRRGAAA
                     RGIGGGLAL"
     gene            1791..>2564
                     /locus_tag="VFF72_04795"
     CDS             1791..>2564
                     /locus_tag="VFF72_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZV92510.1"
                     /translation="MITPWLLAYFATGAFVGFLAGLLGIGGGMTLVPILAAMFTAQHF
                     APDHTVHLALGTGMASIVFTSSASVRSHHRLGGVDWSLVRRMGPGMVAGTLCSTALSG
                     WIPQRILALAFAVIVLAGATQILLGRKPSAARAPPGTPGLVLIGAGIGVVCGLVSAGG
                     AFMTVPFMLFCGVAMTTAIGTGAALGVPVAAMGTLGYVFSGWHVPTVPSLALGFVYVP
                     ALLGIVAGSVLTAPWGARSAHRMPVTTLRRIFAGLLYVLA"
BASE COUNT          364 a          898 c          902 g          400 t
ORIGIN      
        1 gcgcctacgg cctcgtcgcc gagaccggct tctggcccgc ggcgctggcc tggacggaac
       61 gcggcggcgt gctggcgacg gcgcacgttc gcggcggcgg cgagtacggg gaagactggc
      121 acctcggcgg catgatgaag accaagctca acaccgtcta cgacttcatc gcctgcggcc
      181 agtacctcgt cgaccagaaa tacacgacgc cccggcgcct cgcggccgaa ggcggcagcg
      241 ccggcggcat cgcggtgggc cgcgccatga cgatgcggcc cgacctcttc ggcgtgatcc
      301 tcgaccatgt cgggttgtcc gacacgctgc gcttcgagac cgagccgaac gggccaccga
      361 acgtcgtcga attcggctcg gtgaagaccg aagacggctt tcacggcctc tatgcgatga
      421 gcccgtacgc gcacatcgtt gcgggcaccg cctatccggc ggtcatgtac ctgaccggcg
      481 ccaacgaccc tcgtgtcgcg ccatggaaca tgatgaagat ggccgcgcga acgcaggcag
      541 cgacgtcgag ccgaaagccg gtgctgctgc gcatcgacga cgatgccggc cacggcatcg
      601 gctcgaaccg ctcgcagcgc gagcgggagc tggccgacac ctggagcttc gcgctctggc
      661 agatgggcga cccggcgttc cagccgaagt gaaggcggcg ccgtgacgac ggcgcgaacg
      721 caccgtctcc cgccgcgaat tcgccgggtc ctgcccggcg aggtcagggc attctcagag
      781 ggccagaccg ccgccgatgc cgcgcgcggc ggcgccgcga cgccggcggg cgaacatcgc
      841 gagggcgccc aggccgaaca gcatcagggc gtacgtctgc gcctcgggaa ccgccgagac
      901 gctgaagttc gcgacttcga aggcattggt cgccgggttg ttgtcgaaga ccagcgcggt
      961 gatggccgcg ccgtccttgg catggaacca cacgtactgc gagaacgact ggtcgccatc
     1021 ctgcaccgtg aacccgagcg agccggtggt gaacgacacg ttgcccatcg tcgtctcgac
     1081 ggtcagcagg ttgtaggtgt cgggcgagcc ccagaggaag ccgatgtcgg agatgggcga
     1141 cgcgaaggtc atcgtcgccg gctcgtcgtt ggacggcgtc ggcccggcgg acaggaagtt
     1201 gccgccgaac gtgccggcgg gcaacgccgc gaacgacgga tcgcccgtgg tgaagatctt
     1261 gccgccgctg atcgatgcga aggtgccgtc gaggctgcac gggtcggcgc cggcggtacc
     1321 ggtgcaggtg gagcccgtcg tgccgctcag cgccaggtac ggaccggtcc cgcctcccag
     1381 cgtgtaggtg acggtggcgc tcgccccgat cgaggcgaga aggaagaccg atgcggcgag
     1441 gagtcggggg gctttcattg ggatttcgat gccttgttgg aatctgaagc gcgcaggcgc
     1501 ttccacagca tcgacgttat ccgagcgccc ctgccgaacg cgatcccgcg ttcgcggggt
     1561 cgcggttcgc aaatgcaacc ggatcgtccg atttcgccac ttccgacacc gaatcgcggg
     1621 ccatccgcgg atgcggtacc ttctgcggcc atgcccgccg cccttttcgt tgccacctcc
     1681 ccggccggcg gatcgtcgag cgtctccgtg acgcgcgatg cgagccccgg ggcaccgtgc
     1741 gggagcggga ccgcgcacat gacgatcgcc gtcgcttgcg cggcgcaccg atgatcacgc
     1801 cctggctgct cgcctacttc gccaccggcg ccttcgtcgg ctttctcgcc gggctgctcg
     1861 gcatcggcgg gggcatgacg ctggtgccga tactggcggc gatgttcacg gcgcaacatt
     1921 tcgcgcccga ccacacggtg cacctcgccc tgggcacggg catggcgtcg atcgtgttca
     1981 cttccagcgc cagcgtgcgc agccaccatc ggctgggcgg cgtcgactgg tcgctggtgc
     2041 gccgcatggg ccccggcatg gtggccggga cgctgtgctc caccgcattg tcgggatgga
     2101 tcccgcaacg catcctcgcc ctggccttcg cggtgatcgt cctggccggc gcgacccaga
     2161 tcctgctcgg ccgcaagccc tccgcggcgc gcgcgccgcc cggcacaccg gggctcgtcc
     2221 tgatcggcgc cgggatcggc gtcgtctgcg gcctcgtgtc ggcgggcggg gcattcatga
     2281 ccgtgccgtt catgctgttc tgcggcgtgg cgatgaccac cgccatcggc accggcgccg
     2341 cgctgggcgt gcccgtggcg gcgatgggca cgctcggcta tgtgttcagc ggctggcacg
     2401 tgcccaccgt gccttctctc gccctgggct tcgtctacgt cccggccctg ctgggcatcg
     2461 tcgccggcag cgtgctcacg gcaccgtggg gcgcccgctc ggcccaccgc atgcccgtga
     2521 cgacgctgcg gcgcatcttc gccggcctgc tgtacgtcct ggcg
//
LOCUS       DASRWS010000263         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3849768, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000263 DASRWS010000000
VERSION     DASRWS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849768"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VFF72_04800"
     CDS             complement(<1..1607)
                     /locus_tag="VFF72_04800"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZV92511.1"
                     /translation="MEKIWLKQYPQGVPAEIDVTRYASLVALVDDSFRRFAARTAYRF
                     MGRSISFGQVDEASRIFAAWLQSQGLAKGDRVAVMLPNVPQYPVAVAAILRAGLVVVN
                     VNPLYTPRELEHQLKDSGAKVIVILENFAATLAKVLAAVPTKKVVLASMGDMLGFPKG
                     LVVNYVVRKVKKLVPAFDIAGAVRWNDAMAAGRGKTFTPVAVGPDDIAVLQYTGGTTG
                     VSKGAMLLNRNLVANILQSEAWYQPALKKIPAGEQLMTVCALPLYHIFGFNTNMMLSM
                     HMGGANILIANPRDLSGMFKDLQGLKFHAFPAVNTLFNAMANHPDFSTVDWSGLVISV
                     GGGMAVQSATAKLWLEKTGCPIVEGYGLSETSPSATCNPVDSTTYSGNIGLPMPNTEL
                     ELLDDEGREVPMGTPGEIAIRGPQVMAGYWQRPDETAKAMTADGFFRTGDIGTVDERG
                     YFKIVDRKKDMILVSGFNVYPNEVEDVVTQMPGILECAAVGVPDAKAGEAVKLVIVRK
                     DPAITEAMVRAYCEANLTGYKRPKVIE"
     gene            complement(1741..3198)
                     /locus_tag="VFF72_04805"
     CDS             complement(1741..3198)
                     /locus_tag="VFF72_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZV92512.1"
                     /translation="MRLRRFLPASVTARLVLAALALSALGACTVGTPYVRPDAPTPPA
                     YKEAAGWVPAEPADALERGAWWTLFGDAELDRLESKVEVSNQNVAAAVANYAQARALV
                     RVDQAALFPTLGATAGASRSGGGGGSGGVSRSGNSLQAGLDASWAPDVWGRLRLAVSG
                     AEASAQASEADLAAATLSAQALLATDYFALREADAEEALLRRTVAGYERAVTITQNSY
                     DAGISAKTDLLQAQTTLENARATLVGLTHQRAQLEHAIAVLVGLPPADFRIDVAPWVM
                     NVPTVPPGVPSTLLQRRPDIAAAERAVAAANAQIGIQRSAYFPSLSLSGSFGFASSTA
                     SRLFSAPSSLWSVGLSVAQTIFDAGATRARVEGAEAARDAAVAQYRQTVLAAFQNVED
                     QLSASRTLEAQEGSRRRASAAADLVEQQLLNQYRAGQVGYTSVVTAQASALSARQTLV
                     QLVSSRQASAVALIQALGGGWHVPQAFEGAASTPQ"
     gene            complement(3250..>4034)
                     /locus_tag="VFF72_04810"
     CDS             complement(3250..>4034)
                     /locus_tag="VFF72_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZV92513.1"
                     /translation="IYLVPLTSVAKFYETSVPTSVNHQDGELATTISFNLADGRNLND
                     AKAAINQAEADIGMPTNVRGAFAGTAATAQQSQGQQAMLIVAALVVIYIVLGVLYESL
                     IHPLTVLSTLPSAGVGAVLALMALRMEFSIIALIGVFLLIGIVKKNAILIIDFALEAE
                     RSRGLSSLEAVREACLLRFRPILMTTLAAALGALPLAIGFGDGSELRQPLGVAIIGGL
                     IASQLLTLLTTPVVYLLLDKLRRRAPDERHLSRGGGNTYVVA"
BASE COUNT          616 a         1343 c         1444 g          631 t
ORIGIN      
        1 aactcgatga ccttcggtcg cttgtatccg gtgaggttgg cttcgcagta ggcccgcacc
       61 atcgcctcgg tgatcgccgg atccttgcgg acgatcacca gcttgaccgc ctcgccggcc
      121 ttggcgtcgg gcacgccgac ggcggcgcat tcgaggatgc cgggcatctg ggtgacgaca
      181 tcctcgacct cgttcgggta gacgttgaag ccgctgacga ggatcatgtc cttcttgcga
      241 tcgacgatct tgaaatagcc ccgctcgtcg accgtgccga tgtcgccggt gcgaaagaag
      301 ccgtcggccg tcatcgcctt ggccgtttcg tcgggccgct gccagtagcc cgccatcacc
      361 tgcgggccgc ggatggcgat ctcgccaggc gttcccatcg gtacctcgcg gccttcgtcg
      421 tcgagcagct cgagctcggt gttaggcatc ggcaggccga tgttgccgct gtaggtggtg
      481 ctgtcgacgg gattgcaggt ggccgagggc gatgtctccg aaaggccgta gccctcgacg
      541 atcggacagc ccgtcttctc gagccagagc ttggcggtgg cgctttgcac cgccatgccg
      601 ccgcccaccg agatgacgag gccgctccag tcgacggtcg agaaatcggg atggttggcc
      661 atcgcgttga agagcgtgtt gacggccgga aaggcgtgga acttcaggcc ctgcaggtcc
      721 ttgaacatgc cggacaggtc gcgcggattg gcgatcagga tgttggctcc gcccatgtgc
      781 atggacagca tcatgttcgt gttgaagccg aagatgtgat agagcggcaa cgcgcacacc
      841 gtcatgagct gctcgccggc ggggatcttc ttcagcgccg gctggtacca ggcctccgac
      901 tgcaggatgt tggcgacgag attgcggttg agcagcatcg cgcccttgct gacgccggtc
      961 gttccgccgg tgtactggag cacggcgatg tcgtcggggc cgacggcgac cggcgtgaac
     1021 gtcttgccgc ggcccgcggc catcgcgtcg ttccatcgca ccgcgccggc gatgtcgaac
     1081 gccggcacca gcttcttcac cttgcgaacg acgtagttga cgacgagccc cttcggaaat
     1141 ccgagcatgt cgcccatcga cgcgagcacg accttcttcg tcggcaccgc ggccagcacc
     1201 ttcgccagcg tcgctgcgaa gttctcgagg atgacgatga ccttcgcgcc cgaatccttc
     1261 agctggtgct cgagctcgcg cggcgtgtag agcgggttga cgttgacgac gaccagcccg
     1321 gcccgaagga tcgccgcgac ggcgaccggg tattgcggca cgttgggcag catcacggcg
     1381 acgcggtcgc ccttggccag gccctgcgac tgcagccacg cggcaaagat gcgcgacgct
     1441 tcgtcgacct gtccgaacga gatcgacctg cccatgaagc gataggcggt gcgtgcggcg
     1501 aagcggcgaa agctgtcgtc gaccagggcg accagcgacg cgtatcgggt cacgtcgatc
     1561 tcggccggca cgccctgggg atactgtttc agccagatct tttccatcgc agcctctgcg
     1621 gggtttgcgc ggattctggg cggcgcgaac gcgcggggct agggtagttt cgatatgccc
     1681 cccgcgaggg cgacgacgcg acagggaagg aagcgtcggc ggccggcgcg gtcgacgccg
     1741 tcattgcggc gtcgatgcgg ccccttcgaa cgcctgcggc acatgccagc cgccgccgag
     1801 cgcctggatc agggcgacgg cgctcgcctg ccggctgctc accagctgca ccagggtctg
     1861 cctggcgctg agcgccgaag cctgcgcggt gacgacactg gtgtagccga cctggccggc
     1921 ccggtactgg ttgaggagtt gctgctcgac caggtcggcg gcggccgacg cccggcgccg
     1981 cgaaccttcc tgcgcctcga gcgtgcggct ggcgctcaac tggtcctcca cgttctggaa
     2041 cgccgccagt acggtctgcc ggtactgcgc gacggcggcg tcgcgggcgg cctcggcgcc
     2101 ctcgacgcgg gcccgcgtcg ccccggcatc gaagatcgtc tgtgcgaccg agaggcccac
     2161 cgaccagagg ctgctcggcg cactgaaaag cctgctcgcg gtcgagctcg cgaagccgaa
     2221 cgagccgctc agcgacaggc tcggaaaata agccgaacgc tggatgccga tctgcgcatt
     2281 ggcggccgcg accgcgcgct ccgccgcggc gatgtcgggc cgacgttgca gcagggtcga
     2341 cgggacgccc ggcggcaccg tcggcacgtt catgacccac ggcgcgacgt cgatgcggaa
     2401 gtcggccggc ggaagtccca cgaggacggc gatcgcgtgc tcgagctgcg cccgctggtg
     2461 ggtcaggccg accagcgtcg cgcgcgcgtt ctcgagcgtc gtctgcgcct gcagcagatc
     2521 ggtcttggcc gaaatgccgg cgtcgtagct gttctgggtg atcgtcaccg cacgctcgta
     2581 gccggcgacg gtgcggcgca gcagggcttc ttcggcatcg gcctcgcgca gcgcgaaata
     2641 gtcggtggcg agcagcgcct gggcggagag cgtggccgcc gcgaggtcgg cctcgctcgc
     2701 ctgcgcactg gcctcggcgc cgctcaccgc gaggcgcagc cggccccaga cgtcgggcgc
     2761 ccagctcgcg tcgaggccgg cctgcagcga attgccgctg cgcgacacgc cgccgctgcc
     2821 accgccgccg cccgaacgcg acgctccggc ggtcgcgccc aaggtcggga aaagcgccgc
     2881 ctggtcgacg cggacgaggg cacgcgcctg cgcgtagttg gcgaccgcgg cggcgacgtt
     2941 ctggttcgac acctcgacct tcgactcgag tcgatcgagc tcggcatcgc cgaagagcgt
     3001 ccaccaggcg ccgcgctcga gcgcatccgc cggctcggca ggcacccagc cggcggcttc
     3061 cttgtaggcg ggaggcgtcg gcgcgtcggg acgcacgtac ggcgtaccga cggtgcaggc
     3121 gccgagcgcc gacagggcga gggcagcgag gacgaggcgc gccgtcacgg acgcggggag
     3181 gaaacgacgc agtctcatgg ccgatgggga cctgttcgcg cgatggacga gtacgtggcg
     3241 gatttcatcc taggcgacca cgtaggtgtt gccgccgccg cggctgaggt gacgttcgtc
     3301 cggagcgcgg cggcgcagct tgtcgaggag gaggtagacg accggcgtcg tcagcagggt
     3361 cagcagctgg ctcgcgatca gcccgccgat gatggcgacg ccgagcggct ggcgcagttc
     3421 cgagccgtcg ccgaagccga tggccagcgg cagcgcgccg agcgccgccg cgagcgtcgt
     3481 catcaggatc ggccggaagc gcagcaggca cgcttcgcgc accgcctcga gcgagctcag
     3541 cccgcgcgac cgctcggcct cgagcgcgaa gtcgatgatg aggatggcgt tcttcttgac
     3601 gatgccgatc aggaggaaca cgccgatgag ggcgatgatc gagaactcca tgcgcagcgc
     3661 catgagcgcg agcaccgcgc cgacgccggc cgagggcagc gtcgacagca ccgtcagcgg
     3721 atggatcagg ctctcgtaga ggacaccgag gacgatgtag atgacgacca gggcggcgac
     3781 gatcagcatc gcctgctggc cctgcgattg ctgcgcggtc gccgccgtcc cggcgaaggc
     3841 gccgcgcacg ttggtcggca tgccgatgtc ggcttcggcc tggttgatcg ccgccttggc
     3901 gtcgttgagg ttgcgcccgt cggcgaggtt gaacgagatc gtcgtcgcca gctcgccgtc
     3961 ctggtggttg accgaggtcg gcaccgaggt ctcgtagaac ttcgcgaccg acgtcagcgg
     4021 caccaggtag atcg
//
LOCUS       DASRWS010000264         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3850002, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000264 DASRWS010000000
VERSION     DASRWS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850002"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFF72_04815"
     CDS             <1..731
                     /locus_tag="VFF72_04815"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HZV92514.1"
                     /translation="GLLAAALSAAVWGVFRRRNEAAIGRPSGAGSTETLPAQRDDSVD
                     PTRHGTAEVAILAVAYGLAGFGYIVTATFLPVIARTAVPDSSLIDLFWPIVGAGAVAG
                     ALLALRLPTTVDRRWLLAGSLVIQAAGIGLGIALPTVSGFAFGSLLVGIPFAALSLFT
                     LQEARRIRPHAAASTIGLVTVLYAIGQALGPQMVAFVLLRSGGDTPMAFQRSLLIAAM
                     VLLVGTVLAVGSARLWPASGRSPI"
     gene            complement(804..1013)
                     /locus_tag="VFF72_04820"
     CDS             complement(804..1013)
                     /locus_tag="VFF72_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92515.1"
                     /translation="MSESASPNGVYRATPIVEPWGDAGWKAGVLLSGDDARGDRPAKS
                     LCSQLFASREDAWRFAESEYSTLAD"
     gene            complement(1006..1197)
                     /locus_tag="VFF72_04825"
     CDS             complement(1006..1197)
                     /locus_tag="VFF72_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92516.1"
                     /translation="MGLDELTGQYRRLRNELDAAYAAPEWDSDRIDLITEALAPLEVA
                     LASVRKQPPTTTRCGGTHV"
     gene            1422..1946
                     /locus_tag="VFF72_04830"
     CDS             1422..1946
                     /locus_tag="VFF72_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HZV92517.1"
                     /translation="MFKPLRDLLDHLQHLAEPPDPRSAERALQLAAAVMLVEVMRADA
                     SIQPVEREAVHRALREQFSLSADEAASLAELAAQTASHATDLFEFTSRIDAALDMTQK
                     IRIVEHMWHVAYADGALSEDERHILWRIADLLHVPQGAYVNARLRAERAAGLSPIDDS
                     PAESQPHPRLQPEG"
     gene            2081..2338
                     /locus_tag="VFF72_04835"
     CDS             2081..2338
                     /locus_tag="VFF72_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HZV92518.1"
                     /translation="MNDATESTPPPPLPDRLSVDPQSPHHVPAVFERAIGIRLNGKER
                     HDVEEYCLSEGWVKVPAGKTLDRKGRPLLITLKGTVEAFYR"
     gene            2409..2687
                     /locus_tag="VFF72_04840"
     CDS             2409..2687
                     /locus_tag="VFF72_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92519.1"
                     /translation="MRRLFALWRLGREDLRLLVHALRHPSRPAWLWPAAAAMLLYAID
                     PLNFAMPLFGAVDDFVLLPMILHAVLAFLPGDIRASFARSRIPILRPR"
     gene            2814..>4139
                     /locus_tag="VFF72_04845"
     CDS             2814..>4139
                     /locus_tag="VFF72_04845"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HZV92520.1"
                     /translation="MPAHLRKLFAQLGDSFWLLPSGLCLLGIFGAEALVHLDRIGRVP
                     RWLIDGEWVYSGGGTGARTLLGAVASSTIGVAGTVFSITIAALSLAAGQMGPRLLRNF
                     TRDRGVQFTLGAFLGTFSYALMVLRSVRTQDEGAFTPHLSLTVGVLLAFVCVATLVYF
                     VGHMAGRINVDTVIELVSEDVRGAIGRLTVDAPGPAAPAESFWRGATTVVERRRGYLQ
                     ELDTHGLAAWAAEHRSVIRLLVRPGDYVFPGTPAALVLGPADGADAAIRRAMALGATR
                     LSSVDIEFAVRQLVEVAVRALSPGINDPHTAIGVLDRLGASLCDVAPVWLPTGVTLDN
                     GHAVLVVPSVDYDGLVDGMFHMIRQNAAGSAAVLIRLLEVLTAVASCERDPIRSATLQ
                     RHADLALGDAGRSIGTPADVADVRRVHAAFERMRQAGPSGMAGARAAVDE"
BASE COUNT          543 a         1461 c         1430 g          705 t
ORIGIN      
        1 tgggcttgct cgctgcggcg ctgagcgcgg cggtatgggg cgtctttcgg cgccgcaacg
       61 aagcggcgat cgggaggccg agcggtgcgg gcagcacgga aaccttgccg gcgcagcgcg
      121 acgactccgt cgatccgacc cgccacggaa cggccgaggt ggcgatcctg gccgtcgcct
      181 atggcctggc cggcttcggc tacatcgtca ccgcgacgtt tttgccggtg atcgcacgca
      241 ccgccgtccc cgactcgtcg ctcatcgatc tgttctggcc catcgtcggc gccggcgcgg
      301 tggccggtgc gctgctggcg ctgcggctgc cgacgacggt cgatcgccgc tggctcctgg
      361 ccggctccct ggtgatccag gcggccggca tcggcctggg catcgctttg ccgaccgtgt
      421 ccggattcgc gttcggcagc ctgctcgtcg gcatcccttt cgcggcattg agcctcttca
      481 cgctgcagga ggcgcgccgc atccgcccgc atgccgcggc gagcacgatc ggcctggtga
      541 ccgtgctcta cgccatcggc caggcgctcg gcccgcagat ggtcgccttc gttctgctgc
      601 gcagcggcgg cgatacgccc atggccttcc agcgatcgct gctcatcgcc gcgatggtgc
      661 tgctcgtggg aacggtgctg gccgtcggtt cggcgcggct gtggccagcg tcaggaaggt
      721 cgccgatctg atgccgaggg tcgccgcacg cccttcgatg agcggaacga gtcgcagatg
      781 ttcccggcat cggcgctctt gcctcagtcg gcgagcgtcg aatattccga ttctgcgaat
      841 cgccaggcgt cttcgcgcga cgcgaacagt tgcgagcaga ggctcttggc gggtcgatcg
      901 ccgcgggcgt cgtcgccgct gagaaggacg ccagccttcc agccggcgtc gccccagggc
      961 tcgacgatgg gcgtcgcacg gtagacgccg ttgggcgaag cgctttcaga catgggtgcc
     1021 tccgcagcgg gtcgtcgtcg gcggttgctt ccggaccgat gcgagtgcca cctccagcgg
     1081 cgccagcgct tcggtgatga ggtcgattcg atcgctgtcc cattcgggcg cggcataggc
     1141 cgcatcgagt tcgttgcgca ggcggcgata ttggccggtc agttcgtcga gtcccatttc
     1201 tccaaattct ccaggttcca tcgggcggtt ttgttgtgct gcagcatgcc tcgaacccct
     1261 gcgaagatca acggaggaac gagaggaatc ggcatcgtcc tacgacgaaa tcggaagggt
     1321 cgaagcgctg gctcgtgtcg agtggctcgt tggccgacac cgcgtcatgg ccggccttgc
     1381 agaaggcacg gcggagcgcg gggccgtgtc aacatcgcgg catgttcaag ccgctgcgcg
     1441 atctgctcga ccatttgcaa cacctcgccg agccgcccga cccacgtagt gccgagcggg
     1501 cgctgcaact cgctgccgcc gtcatgctcg tcgaggtgat gcgcgccgac gccagcatcc
     1561 agcccgtcga gcgcgaggcc gtgcatcgcg ccttgcgcga gcagttctcg ctgtccgccg
     1621 acgaggcggc cagtcttgcc gagctcgccg cgcagaccgc gtcgcacgcc accgatctct
     1681 tcgagttcac ctcgcgcatc gatgccgcgc tcgacatgac gcagaagatc cggatcgtcg
     1741 agcacatgtg gcacgtcgca tatgccgatg gcgcgttgag cgaggacgag cggcacatcc
     1801 tttggcgtat cgccgacctc ctgcatgtgc cgcaaggcgc ctacgtcaac gcgcggcttc
     1861 gggccgagcg agcggcgggg ctctcgccga tcgacgacag cccggccgaa agccagccgc
     1921 atccgcgact tcagcccgaa gggtagcggc gcgcctggct tcgcgcgggg cggtgccggc
     1981 agcgccgcga tgtcaacgcc gggtcggcga tctgggaggc gcggaccaaa aacgcgtcga
     2041 cgtccgggtg cgtttccgcc tccgacggac aatgcgggcc atgaacgacg cgaccgaatc
     2101 cacgccgccg ccgcctttgc ccgatcgtct ctccgtcgat ccgcagagcc ctcatcacgt
     2161 gccagccgtc ttcgagcgtg ccatcggcat ccggctcaac ggcaaggagc gccacgacgt
     2221 cgaggaatac tgcctgagcg aaggctgggt caaggtgccg gccggcaaga cgctggaccg
     2281 caagggcagg ccgctgctga tcacgctgaa gggaacggtc gaggcgttct accgctagcg
     2341 acgacgaccc tgcccggcgc aagaccccgc tcgcggcagg cgacgtcgcc agcggctacg
     2401 ctgtgccgat gagacgtctt ttcgctctgt ggcggctggg gcgcgaggat ctgcggctgc
     2461 tcgtccatgc cttgcgccac ccgtcgcgtc cggcgtggtt gtggccggcc gcggcagcga
     2521 tgctgctcta tgccatcgat ccgctcaact tcgcgatgcc gctgttcggc gccgtcgacg
     2581 atttcgtcct cctgcccatg atcctgcacg cggtgctcgc cttcctgccc ggcgacatcc
     2641 gcgccagctt cgcccgctcg cgcatcccga tccttcggcc gcgataggcg acgacgacgc
     2701 cgccacggcg gcgggcgtga gcgatcggcg tcggacaacg ccgcgatcca tccgtcgttg
     2761 tcgcgggctg cggcgcgggc cattaggctt ggtcgttcac cacggggcga cctttgcctg
     2821 cccatcttcg aaagcttttc gcgcagctgg gcgactcgtt ctggctgctg ccttccggcc
     2881 tgtgcctgct cggcatcttc ggcgcggagg cgctcgtcca tctcgaccgc atcggccggg
     2941 ttccgcgctg gttgatcgac ggtgaatggg tctactcggg aggcggcacc ggcgcgcgca
     3001 cgctgctcgg cgccgtcgcc tcctcgacca tcggcgtcgc cggcaccgtc ttctcgatca
     3061 ccatcgccgc gctgtcgctc gccgcgggcc agatggggcc gcgcctgctg cgcaacttca
     3121 cgcgcgatcg cggcgtgcag ttcacgctcg gcgctttcct cggcaccttc tcgtatgcgc
     3181 tgatggtgct gcgcagcgtg cgcacgcagg acgagggcgc cttcacgccg cacctgtcgc
     3241 tcaccgtggg cgtcctgctc gcgttcgtct gcgtcgcgac gctcgtctat ttcgtcggcc
     3301 acatggccgg tcgaatcaat gtcgacaccg tgatcgagct ggtgagcgag gacgtgcgag
     3361 gcgcgatcgg gcgcctcacc gtcgatgcgc ccggcccggc ggctcccgcc gaatcgttct
     3421 ggcgcggcgc gacgacggtc gtcgagcgcc gacgcggcta tctgcaggag ctcgacacgc
     3481 acgggctcgc cgcatgggcc gcggagcatc gcagcgtgat ccggctgctg gtgcggccgg
     3541 gcgactacgt ctttccgggc acccccgccg cgctcgtcct cggccccgcg gacggcgccg
     3601 atgcggcgat ccgccgcgcg atggcgctcg gtgcgacgcg gctcagctcg gtcgacatcg
     3661 agttcgcggt gcgccagctc gtcgaggtgg cggtgcgcgc cttgtcgccc gggatcaacg
     3721 acccgcacac cgcgatcggc gtcctcgatc gcctcggcgc gtcgctgtgc gatgtcgcgc
     3781 cggtctggct gccgaccggc gtgacgctcg acaacgggca cgcggtgctg gtggtgccca
     3841 gtgtcgacta cgacggtctc gtcgacggca tgttccacat gatccggcaa aacgccgccg
     3901 gcagcgccgc ggtgctgatc cgcctgctgg aggtgctcac cgcggtggcg agttgcgagc
     3961 gcgacccgat ccgctcggcg acgctgcagc gccacgccga cctcgcgctg ggcgacgccg
     4021 ggcgcagcat cgggacgccc gccgacgtcg ccgacgtccg gcgcgtccat gcggcgttcg
     4081 agcggatgcg ccaggccggg ccatcgggaa tggccggcgc gcgcgccgct gtcgacgag
//
LOCUS       DASRWS010000265         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3850244, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000265 DASRWS010000000
VERSION     DASRWS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850244"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..811)
                     /locus_tag="VFF72_04850"
     CDS             complement(176..811)
                     /locus_tag="VFF72_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HZV92521.1"
                     /translation="MKIDFVSDVACPWCAVGLHSLEKAIANLEGAVPIELHFEPFELN
                     PAMPPEGEDATEHLSRKYGAPPEQLAKSRAAMRERGAAVGFTFGDRPRVWNTFDAHRL
                     LHWAGLESADRQRALKHALLEAYHRDAENPGAHDVLLRLAGAAGLDVERARAILDSDE
                     YAGEVRERERFWQERGIHSVPAIVIDDRHLVSGGQPPEVFEKAIRQIAAGG"
     gene            951..>2246
                     /locus_tag="VFF72_04855"
     CDS             951..>2246
                     /locus_tag="VFF72_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HZV92522.1"
                     /translation="MTSGVIRIRGARQHNLKNLDLDLRTGEMTVVTGPSGSGKSSLVF
                     DTLYAEGQRRYVETFSAYARQFLERMDRPAVDKVEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLFARGGELFDRQTAQRVRHDSPETIHAELLRRAAVPEGMEEPRLVVTF
                     PVELPASTTAAEIEQWLAASGFTRVHAERTAAAPSQPTGVAPNKLLDVVADRFRIGTT
                     EKVRAIEAIETALKRGSGRVNVYAATANGEQLWRFSTGLHCPDSDLRYAEPQPALFSF
                     NSAYGACEACRGFGRVIGVDFGLVIPDHRKTLRSGAIRTIQTPAWKEIQDDLMKYAGE
                     AGIPRDTAWSQLTDAQRAWVVDGSPNWNGKWNQQWYGIKRFFGYLESKAYKMHIRVLL
                     SKYRSYTPCPTCGGARLKLEALLWRLGSKADADAVLPPAR"
BASE COUNT          357 a          801 c          765 g          323 t
ORIGIN      
        1 gcgatcacgc ccaggccgat gccggcgacg aggaggacga ggccgagcac cgcccgcacc
       61 gcgaagcggc cgtagcgcga gcgtgcgagc gccggaatcg aggccagcgc gagcaccagg
      121 ccgatgacgc cgacgacggc ggcgatggcg atcagcggca tcgcgtcgct gcctttcagc
      181 cgccggcggc gatttgccgg atcgcctttt cgaacacctc cggcggctgg ccgcccgaga
      241 cgagatggcg atcgtcgatg acgatcgccg gcaccgagtg gatgcctcgc tcctgccaga
      301 agcgctcgcg ttcgcgcacc tcgccggcat actcgtcgga gtcgaggatg gcgcgggcgc
      361 gctcgacatc gagtcccgcc gcgccggcca ggcgcaacag cacgtcgtgc gcgcccgggt
      421 tctcggcatc gcggtggtag gcctcgagca gggcgtgctt gagcgccctc tggcgatcgg
      481 ccgattcgag tccggcccag tgcagcaggc ggtgcgcgtc gaaggtgttc cagacgcgcg
      541 gccgatcgcc gaacgtgaag ccgacagcgg cgccgcgctc gcgcatcgcg gcgcgcgact
      601 tcgcgagctg ctcgggcggc gcgccgtact ttcgcgacag atgctcggtg gcgtcctcgc
      661 cctccggcgg catcgccggg ttcaattcga acggctcgaa gtgcaactcg atcggcaccg
      721 cgccttcgag gttggcgatc gccttttcga gcgagtggag gcccaccgcg caccacgggc
      781 aggcgacgtc ggaaacgaaa tcgatcttca tcggcgtggc agggcggtca cgatcgacgc
      841 agtctaacgg tggcccccgg ctttcgggcc gacgggtctt tcggcgccgc gccgacgtga
      901 agccgcccgc tccctagaat gctccgctcc cctcgcgaaa gacccttccg atgacatccg
      961 gcgtcatccg catccgcggc gcgcggcagc acaacctgaa gaacctcgac ctcgacctgc
     1021 gcaccggcga aatgacggtc gtgacggggc cgagcgggtc gggaaagtcg agcctcgtct
     1081 tcgacacctt gtatgccgaa gggcagcgac gctacgtcga gaccttcagc gcctatgcgc
     1141 gccagttcct cgagcgcatg gaccggccgg cggtcgacaa ggtcgaaggc gtgccgccgg
     1201 cgatcgccat cgaccagacc aacccggtgc gcagctcgcg ctcgacggtc ggcacgatga
     1261 ccgagctgaa cgaccacctg aagcttctct tcgcgcgcgg cggcgagctc ttcgaccggc
     1321 agaccgcgca gcgcgttcgt cacgactctc cggagacgat ccacgccgag ctgctgcgcc
     1381 gcgccgcggt gcccgagggc atggaggagc cgcgcctcgt cgtcacgttc ccggtcgaat
     1441 tgccggcatc gacgacggcc gcggagatcg agcagtggct cgccgccagc ggcttcaccc
     1501 gcgtccacgc ggagcgcacg gccgccgcgc cgtcgcagcc cacgggcgtg gcgccgaaca
     1561 agctcctcga tgtcgttgcc gaccgctttc gcatcggcac gaccgagaag gtgcgcgcca
     1621 tcgaggcgat cgagacggcg ctcaagcgcg gctcgggccg cgtcaacgtc tatgcggcca
     1681 ccgcgaacgg cgagcagctc tggcgctttt ccaccggcct gcattgtccc gacagcgacc
     1741 tgcgctatgc cgagccgcag ccggcgcttt tttccttcaa ctcggcctac ggcgcatgcg
     1801 aagcctgccg cggcttcggc cgcgtcatcg gcgtcgactt cggcctcgtc attccggatc
     1861 acaggaagac gctgcgttcg ggggcgatca ggacgatcca gacgccggcc tggaaggaga
     1921 tccaggacga cctcatgaag tacgccggcg aggccggcat cccgcgcgac accgcgtggt
     1981 cgcagctcac cgatgcccag cgcgcctggg tcgtcgacgg ctcgccgaac tggaacggca
     2041 agtggaacca gcagtggtac ggcatcaagc gcttcttcgg ctacctcgag tcgaaggcct
     2101 acaagatgca catccgcgtg ctgctctcga agtaccgcag ctacacgcca tgcccgacgt
     2161 gcggcggtgc ccgcctgaag ctcgaggcgc tgctctggcg gctcggctcg aaggcggacg
     2221 ccgatgccgt gctgccgccg gccagg
//
LOCUS       DASRWS010000266         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2691016, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000266 DASRWS010000000
VERSION     DASRWS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691016"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFF72_04860"
     CDS             complement(<1..653)
                     /locus_tag="VFF72_04860"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92523.1"
                     /translation="MLHIRCDGRESRAAPAIARWREVVDDVFSGLQVQPQRESRGFQA
                     AIDLLATPHLRLGTIRGSAHAVCRNAQSARRSDEIGLLLQGTGASRLDLDGISVDLAP
                     GRAVLFDNARPYRFDLGESFEHHLLLIERSSIGFGAGRLSARCGEILPPTLPARLLID
                     ALSRLCTELTTAPGAPVATLAEPLLAWLDVATERPVEKRPRGTDALFARIVADLGRRL
                     "
     gene            820..1839
                     /locus_tag="VFF72_04865"
     CDS             820..1839
                     /locus_tag="VFF72_04865"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZV92524.1"
                     /translation="MIRTAFTVSYRLDHPIACAGMAFAGMTPALPIAVSSAGAMGSLA
                     NVGFFPPPLLEQLVGSIRAATAKPFSINFITLYAQDAIIETAARLAPASVSFHWGRPE
                     PRWVTQLHQAGVRVWEQVGSVEQARRAVDDGIDLVIVQGSEAGGHNYGSMPTLTLLPA
                     VRDALGDEMPILAAGGIVDGRGLAAVLALGADGAWVGTRFVATEESDAHPQWKQRLVA
                     ADGTQTVRTHVFGRHHPAFNPMRVLRNDVVAQWHERVADIPQDNSSERVIGSMDVWGQ
                     SQQLHRFQNLAPMAGASGDFEEMPLLAGQGVGGVHEILPAGIVVERMSHDAQAILQRL
                     AGAAA"
     gene            1836..2717
                     /locus_tag="VFF72_04870"
     CDS             1836..2717
                     /locus_tag="VFF72_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZV92525.1"
                     /translation="MKPVIVAAATGRVGSRVCRRLDDAGIAWVGLSRKPESAAATARQ
                     AGFVPRHGRWAEADLEQPESVRERLADAAAFFLATPDHRRQDEVEIRLIDAASSAGVS
                     HIVKLSAQSARLTPPASFGRLHARSEEALRTSGAKFTILQPVFFLQSLLLFAKPVRGG
                     TLPVAAGAGRVAFVDADDVAGAAVAVLSEPGAHHGKSHVLTGPQAHSFGDVAAALSQR
                     LGHPVRHVSPQRLLARLVLPFLTGMPRWQAHQVVDLLAAIARDAQAVPSDAVRALTGR
                     PPRSLGDFLDREIAVFR"
BASE COUNT          424 a          948 c          953 g          413 t
ORIGIN      
        1 aggcgacgcc caagatcggc cacgatgcgt gcgaaaagcg cgtcggtgcc tcgtgggcgc
       61 ttttcgaccg gccgctcggt agcgacatcg agccacgcga gcaagggctc ggcgagcgtc
      121 gccaccggcg cgccaggcgc cgtcgtgagt tcagtgcaga gccggctcag ggcatcgatc
      181 agcaggcgtg ccgggagagt gggaggaagg atctcgccgc accgcgcact caggcgcccg
      241 gcgccgaagc cgatgctcga tcgttcgatc agcagcagat ggtgctcgaa gctttcgccg
      301 agatcgaagc ggtaggggcg cgcgttgtcg aagagcaccg cccgacccgg ggcgaggtcc
      361 accgagatgc catcgagatc gagacggctc gcccccgtgc cttgaagcag gagaccgatc
      421 tcgtcgcttc gtcgtgctga ctgggcgttg cgacacacgg catgcgcgct gcccctgatc
      481 gtgcccaatc gcaggtgcgg cgtggccagc aggtcgatgg cagcctggaa gccccgcgac
      541 tctcgctgcg gctgcacctg tagcccggaa aacacgtcgt cgacgacttc gcgccagcgg
      601 gcgatggccg gagcggcgcg ggattcgcgc ccgtcgcatc gaatgtgcag cacgcccgtc
      661 tcctcacgga agagtgccgg cgatgcacgc atcgagtgcc ggcaccgcac ggtactacga
      721 ccgagtcgag gttagtttag cgaagcccgc actgccggcg aatctcgagc gcctggggca
      781 acacccgctc ttcggccgcg ccgcgaacca ggagacaaca tgatacgtac cgccttcacc
      841 gtgtcctatc ggctggatca tccgatcgcc tgcgccggca tggcgttcgc cggcatgaca
      901 ccggcgttgc cgatcgccgt ctcctcggcc ggcgcgatgg gctcgctggc caacgtcggc
      961 ttcttcccgc caccattgct cgagcagttg gtcggcagca ttcgtgcggc gacggcgaag
     1021 ccgttctcga tcaatttcat cacgctctac gcgcaggatg cgatcatcga aaccgccgct
     1081 cggctggccc cggcctcggt gtcgttccac tggggccgac ccgagccgcg atgggtcacg
     1141 caattgcatc aggccggcgt gcgggtatgg gagcaggtcg gcagcgtcga gcaggcaagg
     1201 cgcgcggtcg acgacggcat cgacctggtg atcgtgcaag gcagtgaagc cggtggccac
     1261 aactacggct cgatgccgac gctgacgctc ctgccggcag tgcgcgacgc gctcggcgac
     1321 gagatgccga tcctcgccgc cggcggcatc gtcgacggcc gcgggctggc cgccgtgctg
     1381 gcgctcggcg ccgacggtgc ctgggtcggc acgcgcttcg tcgccaccga ggaatcggac
     1441 gcgcacccgc aatggaagca acgcctcgtt gccgccgacg gaacgcagac cgtgcgcacg
     1501 cacgtgttcg gccgccatca tccggcgttc aatcccatgc gggttctgcg caacgacgtc
     1561 gttgcccagt ggcacgagcg cgtcgccgac ataccacagg acaactccag cgaacgcgtc
     1621 atcggctcga tggatgtctg gggccagtcg cagcaactgc atcgattcca gaacctggcg
     1681 ccgatggccg gtgccagcgg cgacttcgag gagatgcccc tgctggccgg ccagggtgtc
     1741 ggcggcgtgc acgagatcct tccggcgggg atcgtggtcg agcgcatgag ccacgatgcg
     1801 caggcgatcc tgcagcgatt ggcgggagcg gccgcatgaa gccggtgatc gttgccgcag
     1861 cgacaggacg cgtcggaagc cgcgtatgcc gcaggctcga cgacgccggc atcgcctggg
     1921 tcggcctgtc gcgcaagccg gaatcggcgg cggccaccgc gcgacaggcc ggtttcgttc
     1981 cacgccacgg ccgctgggcc gaggccgacc tggagcagcc cgaatccgtg cgcgagcggc
     2041 ttgccgacgc cgctgccttc ttcctggcga cacccgatca tcgacgtcag gacgaggtcg
     2101 agatccggct catcgacgcc gcaagctcgg ccggcgtgtc gcacatcgtc aagctgtcgg
     2161 cgcagagcgc gcggctgacg ccgccggcca gcttcggccg cctgcatgca cgcagcgaag
     2221 aggctctgcg caccagcggc gccaagttca cgatcctgca gccggtgttc ttcctgcaat
     2281 cgcttctgct gttcgcaaag ccggtgcgcg gcggcacgct ccccgtggca gccggcgcgg
     2341 gacgggtcgc cttcgtcgat gccgacgacg tggcaggcgc tgcggtagcc gtgctgagcg
     2401 agccgggcgc ccatcatggc aagagtcatg tgcttaccgg gccgcaggcg cactcgttcg
     2461 gcgacgtggc ggcagcgctc tcgcaacggc tcgggcaccc ggttcgacac gtcagtccgc
     2521 aacggctgct ggcgcgcctg gtgctgccgt tcctcaccgg catgccacgc tggcaggccc
     2581 atcaggtggt cgacctcctg gccgcgattg cgcgtgacgc ccaggccgtt ccgagcgacg
     2641 ccgtgcgggc actgaccggg cggccgcccc gcagcctcgg cgacttcctc gatcgcgaga
     2701 tcgcggtgtt ccggtgaccg cggcgtcgac gaggctcg
//
LOCUS       DASRWS010000267         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_690989, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000267 DASRWS010000000
VERSION     DASRWS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690989"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFF72_04875"
     CDS             <1..363
                     /locus_tag="VFF72_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92526.1"
                     /translation="GGAGVLMLEKLWAANLALRRRFPDGVEPFKIIARLAEECGELSA
                     EVQRWEGMGVKRQKHGPPDREQLAKEVMDVLRAGLAIAEYYGIESELDAALDRSIAYA
                     LSVGQLSPDEARGVRPGP"
     gene            complement(368..688)
                     /locus_tag="VFF72_04880"
     CDS             complement(368..688)
                     /locus_tag="VFF72_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV92527.1"
                     /translation="MANITYIEHDGTEHVVDVKPGLSVMEGAVKNNIPGIDADCGGAC
                     ACATCHVYVDEAWLARTGSQSAMEESMLDFAEQVEPNSRLSCQIKVTDELDGLVVRMP
                     ESQH"
     gene            complement(710..1942)
                     /locus_tag="VFF72_04885"
     CDS             complement(710..1942)
                     /locus_tag="VFF72_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZV92528.1"
                     /translation="MSETAPHVVIVGAGHAGGTAAALLRQYGFAGHVTLVGEEPIPPY
                     QRPPLSKAWLKGEADADALTLKPESFYAEHDIDLRMGVCVTAIDRAARTVTLDDGRSV
                     AYDIMIIATGARARTLPIPGADLDGVLSLRSAANAEALKAALGPGKRMAVIGGGYIGL
                     EAAASGRALGAEVVVLEREANILARVAKEPLASFFADYHRAHGVTFEVNAQVEAFVGE
                     AGHVAGVRLGDGRTIPCDIALVGVGLVRNDDLAKAAGLDCNNGVIVDETARTSDPNIF
                     AIGDVTHRPMPHYGGRMTGLESVANALEQAKQAASFIAGRPAPPPEVTWNWSDQYDLK
                     LQMAGAAFDVDSTLVRGDPKSAKFAVFHLNGDVIQAVEAVNAPAEFMAGRQLIASRKP
                     ISREKLADPSISMKEVAA"
BASE COUNT          309 a          693 c          707 g          339 t
ORIGIN      
        1 ggtggagctg gagttctgat gctggagaag ctttgggccg ccaatctcgc tctgcggcgg
       61 cgattccccg atggggtcga gccgttcaag atcatcgcgc ggctcgccga ggaatgcggc
      121 gagctttccg ccgaggtgca acgctgggag ggcatgggcg tcaagcgcca gaagcacggg
      181 ccccccgacc gcgagcaact ggcgaaagag gtcatggacg tcctgcgcgc cgggctcgca
      241 atcgccgagt attacggcat cgagtccgaa ctggacgcgg ctctggaccg ctcgatcgcc
      301 tacgcgctct cggtcggtca gctcagccct gacgaagcga ggggcgtgcg gcctggcccc
      361 tagctagcta gtgctggctt tccggcatgc ggacgacgag gccgtccagc tcgtccgtca
      421 ccttgatctg gcaggaaagg cgcgagttcg gctcgacctg ttcggcgaag tcgagcatcg
      481 actcctccat cgccgactgg ctgccggtcc tggccagcca cgcctcgtcc acatagacgt
      541 ggcacgtcgc gcaggcgcag gcgcccccgc agtccgcatc gatgcccggg atgttgttct
      601 tcaccgcgcc ttccatgacc gaaagcccgg gcttcacgtc gacgacgtgc tcggttccgt
      661 catgttcgat gtaggtgatg ttcgccatac gcttttcgct tcgcctccct taggcggcga
      721 cctctttcat ggaaatggac ggatcggcaa gcttctcgcg cgaaatcggc ttgcgggaag
      781 cgatgagctg acggccggcc atgaattcgg ccggtgcgtt cacggcctcg acggcctgga
      841 tgacgtcgcc gttcagatgg aagacggcga acttcgcgga cttcgggtcg ccgcgcacga
      901 gggtcgaatc gacgtcgaac gccgccccgg ccatctgcag cttgaggtcg tactggtccg
      961 accagttcca cgtgacctcc gggggcggcg ccggccgccc cgcgatgaag ctcgccgcct
     1021 gcttggcctg ttcgagggcg ttggcgacgc tctccagccc ggtcatgcgc ccgccgtagt
     1081 gcggcatcgg ccgatgggtg acgtcgccga tggcgaagat gttgggatcg ctggtgcgcg
     1141 ccgtctcgtc gacgatgacg ccgttgttgc agtcgaggcc ggccgccttg gccaggtcgt
     1201 cgttgcgcac gagcccgacg ccgaccagcg cgatgtcgca agggatggtg cggccgtccc
     1261 ccaatcgaac gccggcgacg tggcccgctt cgcctacgaa ggcttcgacc tgggcgttca
     1321 cctcgaacgt cacgccgtgc gcccgatgat agtcggcgaa gaaggaggcc agcggctcct
     1381 tggccacgcg ggcgaggatg ttggcctcgc gctcgagcac caccacctcg gcgccgagcg
     1441 cgcgccccga cgccgccgcc tcgaggccga tgtagccgcc gccgatgacc gccatccgtt
     1501 tgccgggacc cagcgccgcc ttcagcgcct cggcgttcgc cgccgagcgc agggagagca
     1561 cgccgtcgag atccgcgccg gggatcggca gcgtccgcgc ccgcgcgccg gtggcgatga
     1621 tcatgatgtc gtaggccacc gagcggccgt catcgagcgt tacggtgcgc gccgcgcgat
     1681 cgatggcggt gacgcaaacg cccatgcgca ggtcgatgtc gtgctcggcg tagaagctct
     1741 ccggcttcag cgtcagggcg tcggcgtcgg cttcgccctt gagccaggcc ttggagagcg
     1801 gcggccgctg atagggcggg atcggctcct cgccgaccag ggtgacgtgg cccgcgaagc
     1861 cgtactggcg cagcagcgcg gcggccgtgc cgccggcatg gccggcgccg acgatcacca
     1921 catgcggggc ggtctcgctc atacggctcc gatgcagaag aatgacggag gcgtcaactt
     1981 accgctccga catgtttggc gaaggccgcc gcaagagcct gcgctcagac gacgcgctcg
     2041 accatcat
//
LOCUS       DASRWS010000268         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_413490, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000268 DASRWS010000000
VERSION     DASRWS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413490"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFF72_04890"
     CDS             <1..287
                     /locus_tag="VFF72_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZV92529.1"
                     /translation="SRYRPGDDWQKFPLGDPIARLKQHLIGLGQWSNEEHEATQKALE
                     AEVSAALKEAATFGSLLDGHIPPIETMFEDVYKEMPAHLRDQLRQAEEKS"
     gene            382..1386
                     /locus_tag="VFF72_04895"
     CDS             382..1386
                     /locus_tag="VFF72_04895"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZV92530.1"
                     /translation="MTMIQALRSAMDVMLGRDEDVVVYGQDVGYFGGVFRCTEGLQAR
                     YGRSRVFDAPISEGGIVGTAVGMAAYGLRPVVEVQFADYFYPASDQIVSEAARLRYRS
                     AGDFTAPITIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIASIE
                     NDDPVIFLEPKRLYNGPFDGHHDRPVVPWSAHPSSEVPEGHFTVPLDTASIFRAGRDV
                     TVLTYGTMVFVSEAAARDSGIDAEIIDLRTLWPLDLPTVVASVRKTGRCVVVHEATRS
                     SGFGAELAALVQEHCFYHLEAPIERVAGWDTPYPHALEWDYFPGPSRVAAAMRRVLEP
                     "
     gene            1463..>2778
                     /locus_tag="VFF72_04900"
     CDS             1463..>2778
                     /locus_tag="VFF72_04900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZV92531.1"
                     /translation="MSLHVIKVPDIGEGIAEVELVAWRVKAGDDIADDQILADVMTDK
                     ATVEIPSPVAGRVLSLGGEVGQMLAVGADLIRIDAVADTGALRPSAAVMNTGTNATQP
                     AAAAATTVSPAATVAPSAGARLSVQSTPSPTLATPGTPPPSTPAPGTPASSVSSAAAP
                     HRFVPPPSPASTPSPAESLSASSHDGARPIASPALRARAWERGIDLRDVVPSGPGGRV
                     MQRDLDAYGGPTRVPHLASASASVGAAAPSIGAATALASAASRHFGAGREPLAEGQPA
                     SATHAEKIVGLRRKIAQKMQEAKRRIPHFSYVEEIDVGEIEALREQLNAERGSSRGHL
                     TLLPFLVRAIVLAAREVPQVNALFDDEAGVVTRHDAVHLGIATQTENGLMVPVLRHAE
                     ARDLWDNAAEIARLAGAARSGKIVRDELAGSTLTLTSLGRLGGIVST"
BASE COUNT          431 a          998 c          935 g          414 t
ORIGIN      
        1 cctcgcgcta tcgaccgggc gacgactggc agaagtttcc gctcggcgat ccgatcgccc
       61 gcttgaagca gcacctgatc ggcctcggcc aatggtcgaa cgaggagcac gaggcaacac
      121 agaaagcgct cgaggccgag gtgtctgcgg cgctgaagga ggcagcgacc ttcggctcgc
      181 ttctcgatgg ccacattccg ccgatcgaga cgatgttcga ggatgtctac aaggagatgc
      241 cggcgcatct gcgcgaccag ctccggcaag ccgaggaaaa gagctgatgc gaaagcccgt
      301 gcacaaagcg gatctgcgtg gccgcgccgc gatcgcctct ccgcggcgtg gcagccgcca
      361 ggaggagcgc ccgtgccggt gatgaccatg atccaggccc tgcgctcggc catggacgtc
      421 atgctcggcc gcgacgagga cgtcgtcgtc tacgggcagg acgtcggcta cttcggcggc
      481 gtcttccgct gcaccgaagg gctccaggcc cgatacggaa ggtcgcgtgt cttcgacgcg
      541 ccgatttccg aaggcggcat cgtcggcacg gctgtcggca tggcggcata cggcctgcgg
      601 ccggtcgtcg aggtgcagtt cgccgactat ttctatccgg cttccgacca gatcgtctcc
      661 gaggccgcgc ggttgcgcta ccggtcggcg ggcgacttca ccgcgccgat cacgatccgc
      721 atgccctgcg gcggcggcat ctatggcggc cagacgcaca gccagagccc ggaggcgctg
      781 ttcacgcatg tctgcggact gcgcacggtc atgccctcga acccgtacga cgccaagggc
      841 ctgctcatcg cctcgatcga gaacgacgat ccggtcatct tcctcgagcc caagcgcctc
      901 tacaacggtc ccttcgacgg ccaccacgac cggcccgtgg tgccgtggtc ggcacacccg
      961 tcgagcgaag tgccggaagg gcatttcacg gtgccgctcg acaccgcgtc gatctttcgc
     1021 gccggccgcg acgtcacggt gctgacctac ggcacgatgg tcttcgtcag cgaggcggcg
     1081 gctcgcgaca gcggcatcga cgccgagatc atcgatctgc gcacgctctg gccgctcgat
     1141 ttgccgaccg tcgtcgcgtc ggtcaggaag acgggccgat gcgtcgtcgt ccacgaggcc
     1201 acgcgcagca gcggcttcgg cgcggagctc gcggcactcg tccaggagca ttgcttctac
     1261 catctcgagg caccgatcga acgcgtcgcc ggatgggaca cgccctatcc gcacgcgctc
     1321 gagtgggatt actttcccgg cccgtcccgt gtcgccgccg ccatgcgccg cgtgctcgag
     1381 ccatgagcgc cagcgcacga cgcactcact cgtgcctgtc cgcgacgccc ggtgcggcgg
     1441 cgcccgcgcg gaggaagctt tcgtgagcct gcatgtcatc aaggtgccgg acatcggcga
     1501 gggcatcgcc gaagtcgagc tcgtcgcatg gcgcgtgaag gcgggcgatg acatcgccga
     1561 cgaccagatc ctcgccgacg tcatgaccga caaggcgacc gtcgagatcc cgtcgccggt
     1621 ggccgggcgg gtcctgtcgc tcggtggaga ggtcggccag atgcttgcgg tcggtgccga
     1681 cctgatccgc atcgatgccg tcgccgatac gggcgccttg cgcccttcag cggctgtgat
     1741 gaataccggc acgaatgcca cgcaacctgc ggctgccgcg gcaacgacgg tttcgccggc
     1801 agcgaccgtc gccccctcgg ccggcgcacg gctgtcggtt cagtcgacgc cgtcgccgac
     1861 gctggcaacg cccggcacgc caccgcccag cacgcccgcc cccggcacgc cagcgtccag
     1921 cgtgtcttcg gcagcggcgc cccatcgttt cgtgccaccg ccatcgccag cctcgacacc
     1981 ttcaccggcc gaatctctct ctgcatcgag ccacgacggc gcccgcccga tcgcctcgcc
     2041 tgcgcttcgt gcccgcgcct gggagcgcgg catcgacctg cgcgacgtgg tgccgagcgg
     2101 accgggcggc cgcgtcatgc agcgcgacct cgatgcgtac ggtggcccga cccgcgtgcc
     2161 gcacctggcg tccgcgtcgg cgtcggtcgg ggctgccgcg ccgtccatcg gcgcggcgac
     2221 ggcgctcgcg agcgcggcgt cacgtcactt cggtgccggc cgggaacctc tcgccgaagg
     2281 tcagcccgcg agcgcgacgc atgccgagaa gatcgtcggc ctgcgccgca agatcgcgca
     2341 gaagatgcag gaagcgaagc ggcggatccc gcacttcagc tatgtcgagg agatcgacgt
     2401 cggcgagatc gaggcgctgc gcgagcagct gaacgccgag cggggaagca gccgcggcca
     2461 cctgaccttg ctgcccttcc tggtgcgggc gatcgtcctg gcggcgcgcg aggtgccgca
     2521 ggtcaacgcg ctcttcgacg acgaggccgg cgtcgtaacc cggcacgacg ccgtccacct
     2581 cggcatcgca acgcagaccg aaaatggcct gatggtgccg gtgctccggc atgccgaagc
     2641 acgcgatctc tgggacaacg ccgccgagat cgctcgcctg gccggggcgg cacgaagcgg
     2701 gaagatcgtt cgcgacgagc tcgccggctc gaccctgacg ctcaccagcc tcggccggct
     2761 cggcggcatc gtctcaac
//
LOCUS       DASRWS010000269         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1552166, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000269 DASRWS010000000
VERSION     DASRWS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552166"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..694
                     /gene="rpsD"
                     /locus_tag="VFF72_04905"
     CDS             71..694
                     /gene="rpsD"
                     /locus_tag="VFF72_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZV92532.1"
                     /translation="MARYLGPKAKLSRREGTDLFLKSARRPISDKAKFDSKPGQHGRT
                     SGSRTSDFGLQLREKQKVKRMYGVLEKQFRRYFEEAERRKGNTGANLLSLLESRLDNV
                     VYRMGFGSTRAEARQLVSHRAITVNEVVVNIASYSVKAGDTVAVREKAKKQLRITDAS
                     KLAEGQGLADWVQVDSAKLEGVFKKAPDRDQFGADIKEALIVELYSR"
     gene            930..1916
                     /gene="rpoA"
                     /locus_tag="VFF72_04910"
     CDS             930..1916
                     /gene="rpoA"
                     /locus_tag="VFF72_04910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZV92533.1"
                     /translation="MQTNLLKPKAINVEPLGDNKAKVTLEPFERGYGHTLGNALRRVL
                     LSSMVGYAPTEVTIAGVLHEYSAIDGVQEDVVHIMLNLKGVVFRLHNRDEVTLVLRKE
                     GEGPVKAGDIQTPHDVEIINPDHEIAHLAAGGKLDMQIKVEKGRSYVPGNLRRYGDEP
                     TKSIGRIVLDASFSPVARVSYTVDSARVEQRTDLDKLVMEISTNGAITPEEAIRSSAK
                     ILVEQLAVFAQLEGQIEGIFDPQPVRSTQFDPILLRPFDELELTVRSANCLKAENIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGARLENWPPQGLDKR"
     gene            2044..2439
                     /gene="rplQ"
                     /locus_tag="VFF72_04915"
     CDS             2044..2439
                     /gene="rplQ"
                     /locus_tag="VFF72_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZV92534.1"
                     /translation="MRHGHGLRKLNRTSEHRLAMLRNMCVSLLRHEAIKTTLPKAKEL
                     RRVVEPLITLAKEPTLAHRRLAFDRTRDREIVAKLFNELGPRYKARPGGYTRILKMGF
                     RVGDNAPMAFVELVDRPAPVEAEAADEKA"
     gene            2456..3376
                     /locus_tag="VFF72_04920"
     CDS             2456..3376
                     /locus_tag="VFF72_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV92535.1"
                     /translation="MPAPLLRIAHLTKKFGSATVVDDLSLEIMPGECLGVIGPNGAGK
                     TTTIRICLGLVGPDGGEIEAFGLPLPAQVREAKKRIGVVTQFDSLDPDFTCAENLRVY
                     GSYFGLPRDVIDERVPRLLDFAALQSKAAARPGELSGGMKRRLSLGRALINDPDLLIL
                     DEPTTGLDPQARHLMWERLQNLLKQGKAILLTTHFMDEAERLCDRLLVLDHGRKIAEG
                     TPRMLIAEHLEKDVVEVYGDGAGRAASAHGHLAERVETSGETVFFYLRDAKPLLDALA
                     DDHGVRTLHRPANLEDLFLKLTGRQIRDDA"
     gene            3486..>3732
                     /locus_tag="VFF72_04925"
     CDS             3486..>3732
                     /locus_tag="VFF72_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NodJ"
                     /protein_id="HZV92536.1"
                     /translation="MISFYAAPRLSRRFWPVVMRNMLVWKKLAGPSILGNIAEPLITL
                     VAFGYGVGSLIGHVDGMPYIKYLASGTVAVSAALAATF"
BASE COUNT          673 a         1311 c         1187 g          561 t
ORIGIN      
        1 cccaccgccg ccggcacgcc gccgatggct cccgcagttc tctgcggcag atgacataga
       61 aggaaatcac gtggcacgct acctcggccc caaggccaaa ctctcccgcc gcgaaggcac
      121 cgacctgttc ctgaagagcg cacggcgccc gatcagcgac aaggccaagt tcgactccaa
      181 gcccggccag cacggccgca cctcgggttc gcgcacttcc gacttcggcc tgcagcttcg
      241 cgagaagcag aaggtcaagc gcatgtacgg cgtgctcgag aagcagtttc gccgctactt
      301 cgaggaagcc gagcggcgca agggcaacac cggggcgaac ctgctgtcgc tgctcgagtc
      361 gcgcctcgac aacgtcgtct atcgcatggg tttcggctcg acgcgtgccg aggcacgcca
      421 gctggtctcg caccgcgcca tcaccgtcaa cgaggtcgtc gtcaacatcg cgtcgtactc
      481 ggtgaaggcg ggcgacacgg tcgccgtgcg cgagaaggcg aaaaagcagc tgcgcatcac
      541 cgacgcctcc aagctcgccg aaggccaggg cctggccgac tgggtccagg tcgattccgc
      601 gaagctcgag ggcgtcttca agaaggcacc cgatcgcgac cagttcggtg ccgacatcaa
      661 ggaagcgttg atcgtcgaac tctattcgag gtagcacgcg ccgcgcgtcg cgcggcccgg
      721 tcttgcatct gccctgtttc gccgtccctt cgggcggcag cgccgccgac gcaacctcga
      781 tgccgcccgc cgcgtgttcg ggtgtttttt ccaacggttc aggccgctgc cctcttgccg
      841 ggcacgcagc ggcgacatct ccagcccggc gcggtccatc agccttatcg gtgtaacgag
      901 ccgagggtat tgagaggaac aggacttcaa tgcaaacgaa tctgctgaaa cccaaggcca
      961 tcaacgtcga gccgctcggc gacaacaagg ccaaggtcac cctcgagccg ttcgagcgcg
     1021 gctacggtca taccctcggc aacgcgctgc gccgcgtcct gctgtcgtcg atggtcggct
     1081 acgcgccgac cgaagtgacg atcgccggag tgctccatga gtactcggcc atcgacggcg
     1141 tccaggaaga cgtcgtccac atcatgctca acctgaaagg cgtcgtcttc cgcctgcaca
     1201 accgcgacga agtgacgctg gtgctgcgca aggaaggcga aggcccggtc aaggccggcg
     1261 acatccagac gccgcacgac gtcgagatca tcaaccccga ccacgagatc gcccatctcg
     1321 ccgccggcgg caagctcgac atgcagatca aggtcgagaa gggccgcagc tacgtgccgg
     1381 gcaacctgcg ccgctacggc gacgagccga ccaagtcgat cggccggatc gtcctcgatg
     1441 cgtcgttctc gccggtcgcg cgcgtcagct acaccgtcga cagcgcgcgc gtcgagcagc
     1501 gcaccgacct cgacaagctg gtgatggaga tctccaccaa cggcgcgatc acgcccgaag
     1561 aggcaatccg ctcgtcggcc aagatcctgg tcgagcagct ggccgtcttc gcgcaactcg
     1621 aagggcagat cgagggcatc ttcgatccgc aaccggttcg ctcgacgcag ttcgatccga
     1681 tcctgctgcg cccgttcgat gagctcgagc tcacggtgcg ttcggcgaac tgcctgaagg
     1741 ccgagaacat ctactacatc ggcgacctca tccagcgcac cgagaccgag ttgctgaaga
     1801 cccccaacct cggccgcaag tcgctcaacg agatcaagga agtgctggcc tcgcgtggcc
     1861 tcacgctcgg cgcccggctc gagaactggc cgccgcaagg gctcgacaag cggtagcgct
     1921 tcgcgaacag gaaaaacggc cgcaaggccg ttcaaggtgg tcggcccttc ggggccggtc
     1981 ggtgcacccg ccagtacctg acacggctgg cggctcataa gcaagactga aaggaaaagc
     2041 accatgcgtc acggacacgg actccgcaag ctcaatcgca cctccgagca tcggctcgcg
     2101 atgctgcgca acatgtgcgt ctcgctgctt cgccacgaag ccatcaagac gacgctgccc
     2161 aaggccaagg agctgcgccg cgtcgtcgag ccgctcatca cgctggccaa ggagccgacg
     2221 ctcgcgcacc gccggctggc gttcgaccgc acccgcgatc gcgagatcgt cgccaagctc
     2281 ttcaacgagc tgggcccgcg ctacaaggcg cgcccgggcg gctacacccg catcctgaag
     2341 atgggctttc gcgtcggcga caacgcgccg atggccttcg tcgagctcgt cgaccggccg
     2401 gcgccggtcg aggccgaagc cgccgacgag aaggcctgat atcgaggggc gagccatgcc
     2461 ggcgccgctg ctgcgcattg ctcacctgac gaagaagttc ggcagcgcga ccgtcgtcga
     2521 cgatctgtcg ctggagatca tgcccggcga atgcctgggc gtgatcggcc ccaacggcgc
     2581 cggcaagacg accacgatcc ggatctgcct gggcctcgtc ggccccgacg gcggcgagat
     2641 cgaagccttc ggcctgccgc tgccggcgca ggttcgggaa gcgaagaagc ggatcggcgt
     2701 cgtcacccag ttcgacagcc tcgaccccga tttcacctgc gccgagaacc tgcgcgtcta
     2761 cggctcctac ttcggcttgc cgcgcgacgt gatcgacgag cgcgtgcccc ggctgctcga
     2821 cttcgcggcg ctgcaatcga aggcggccgc gcggcccgga gagttgtcgg gcggcatgaa
     2881 gcggcggctt tcgctcggcc gcgcgctcat caacgatccc gatctgctga tcctcgacga
     2941 gccgacgacc gggctcgatc cgcaggcgcg ccacctgatg tgggagcggc tgcagaacct
     3001 gctcaagcaa ggcaaggcga tcctgctgac gacgcacttc atggacgaag ccgagcggct
     3061 gtgcgaccgg ctgctggtgc tcgatcacgg caggaagatc gccgaaggca cgccgcgaat
     3121 gctgatcgcc gagcacctcg agaaggatgt cgtcgaggtc tacggcgacg gcgccggccg
     3181 tgccgcatcg gctcacggcc atctcgccga gcgtgtcgag acgagcggcg agacggtgtt
     3241 cttctacctg cgcgacgcca agccgctgct cgacgcgctg gccgacgacc acggcgtgcg
     3301 cacgctgcat cgaccggcca atctggagga cctgttcctc aagctgacgg gccggcagat
     3361 ccgggacgac gcatgagcct tgccacgacg tcgacgtcga cgagcgcgat cgctgctgct
     3421 gccaagggcg cggccggtcg tgacgccgcc ctgcgcccgc cagccaagcc ccggcgcttg
     3481 cgccgatgat ttccttctac gccgccccgc gtctgtcgcg ccgcttctgg ccggtggtga
     3541 tgcgcaacat gctcgtctgg aagaagctgg cgggcccgag catcctgggc aacatcgccg
     3601 agccgctgat cacgctcgtc gccttcggct acggcgtcgg ctcgctgatc ggccacgtcg
     3661 acggcatgcc ctacatcaag tacctcgcct cgggcaccgt cgcggtgagc gccgcgctcg
     3721 ccgccacgtt cg
//
LOCUS       DASRWS010000270         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1824847, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000270 DASRWS010000000
VERSION     DASRWS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824847"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..1574
                     /locus_tag="VFF72_04930"
     CDS             348..1574
                     /locus_tag="VFF72_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92537.1"
                     /translation="MIPSSRAAHVQPRRVEPALIVIAAGVCAALHVGKLPPAIATLRE
                     ALGMSLVQAGFLLSLVQVAGMTCGVAFGVVADGLGLRRSMMLGLVVLAVASALGGFSQ
                     GVAMLMVLRASEGFGFLLVVLPAPGLVRRLVAPQRVSAMMGLWGAYMPLATALALLIG
                     PLCIGAWGWRAWWWALAAVSLAMAVVLWRCVPVTLAAAPDVATAPWLQRLQRTLAVPG
                     PWLVALTFATYSGQWLAVIGFLPAIYTQAGVSGVATGLLTALAAAANIVGNVAAGRLL
                     QRGVAPARLLVIGFVTMAVASAATFAGVAGDGLPAWARFVAVLLFSGVGGLIPATLFA
                     LAVRVAPAEGTLSTTIGWMQQWSALGQFFGPPLVAWVASRTGGWQFTWLATGACSVGG
                     LVLAGALTRVGRLQRA"
     gene            complement(1659..2507)
                     /locus_tag="VFF72_04935"
     CDS             complement(1659..2507)
                     /locus_tag="VFF72_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HZV92538.1"
                     /translation="MKTPQRLRPLLEEGLIDSVVRQLMSGKEAMVYVVRCGDETRCAK
                     VYKEATQRSFRQAVDYTENRKVKNTRQARAMAKGTRFGREAQEAAWQSAEVDALHRLA
                     TAGVRVPRPINFHDGVLLMELVVDAEGGAAPRLNDVDFSAEEARAHHATLMGEVVRML
                     CAGVIHGDLSEFNILLAADGPVIIDLPQAVDAAGNNHAQRMLLRDVANLRDFFGRFDP
                     SLLATQYGPEIWALYTRGVLQPATPLTGRFEPGHRPVDVQGVLRVVADARDEETARLL
                     RMGTRE"
     gene            complement(2504..2866)
                     /locus_tag="VFF72_04940"
     CDS             complement(2504..2866)
                     /locus_tag="VFF72_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92539.1"
                     /translation="MHDFYDHNQTLVPESFLAIHSRNGRALGSRAGIEARYGLCEDVA
                     LHAAAFLAARHQEGDDTSAALRRCHEGLRADPQAFAAAEAAWVVRRVAELQEWDVPAW
                     LSDPDGDAGTGTARSGRA"
     gene            complement(2969..3439)
                     /locus_tag="VFF72_04945"
     CDS             complement(2969..3439)
                     /locus_tag="VFF72_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZV92540.1"
                     /translation="MARSTFVYVTYIRTTPQKLWHALTDAEFTTQYWFGMRSESEWRA
                     GAPWKLVARDGAVWDSGSVVEADPPRRLVIRWQHQIKPELKAEGDTICTMEIEPTDDG
                     TCKLAVTHSCEREHAKVIEAVSGGWPKVLSNLKSVLETGAATMREPSPAMQAKA"
     gene            complement(3432..3764)
                     /locus_tag="VFF72_04950"
     CDS             complement(3432..3764)
                     /locus_tag="VFF72_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZV92541.1"
                     /translation="MAAARDHDALFKALADPSRRKLLDLLHASDGRTLNELCEHLDMT
                     RQGVTQHLGVLEAANLVATVRQGREKLHFLNPVPLQSLYERWIAKFEKPRLKALSDLK
                     LRLEKPHG"
     gene            3908..5626
                     /locus_tag="VFF72_04955"
     CDS             3908..5626
                     /locus_tag="VFF72_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZV92542.1"
                     /translation="MITTLVTLLRDKLVGRADALVSGHPLARPLPSWRDALARIGRLA
                     VLAGIALLATACATLPPPAHPAPTQAIADYAQTPLGEMTRAVLPTDGRSGFLLQPYGP
                     NAFATRLALTRLATRSLDVQYYLLQGDNAGRSLMRALRDAAARGVRVRVLVDDLYTVG
                     EDELLLGLAAYPNVEVRLFNPFPAGRADEVSRFIASGLDFGRVNRRMHNKLFVADNAA
                     AVAGGRNMADEYVSNTLGRNFIDMDVFAAGPVVRALSAEFDHYWNSDLVFAVDRIATS
                     PLGRAELQARFERATRDATPPEAGAVAADGKVDHPIDGEPTSVSPDLAAMLNLPFELA
                     SHRIGPLLPADARVLFDPLTKTRGWNEREDSIAGTVTAGVIGWFRTARVSIKMVSPYF
                     VPNDAAVASLSEAVASGLDVEVVTNSLASTDEPLVYFGYWPHVAALLKAGVKIRELSP
                     SLSAERHRLGLFGRRTGALHMKNAIVDHRQVFLGSMNLDQRSAKLNTELGLIIDSAEM
                     ARQLEAMTDSGSTYRLRLNAGGGVEWVADEGDGKTRVYDAPPETTAWQRFKTRLLSVF
                     VPAGEL"
     gene            complement(5726..>6136)
                     /locus_tag="VFF72_04960"
     CDS             complement(5726..>6136)
                     /locus_tag="VFF72_04960"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HZV92543.1"
                     /translation="GRDVDPASNRLGGIQLGLFDVSDASQPKSLATLVVGASGSQTAL
                     DASSHGINWLQSGNVARIGLPMIITAAPGDPSPQHGLQRIEVDTQAQSLALKSLLPAP
                     AGSMTYPSLWGERSLQIGSKLVYLTGGQLVVADW"
BASE COUNT          767 a         2206 c         2174 g          989 t
ORIGIN      
        1 cggcgctgcc gaccattacc tgaggctcgc gatctaccac gccacgcatc gtgtgtacgc
       61 ggggctgtcg ctggtcgcat tgctgaccgt ggtggtcgtg ctcctgacgc cgcgccattt
      121 tccggagagc acgcgcctcg acccggcgga cgcgaaaggc gtcgcgggct gaaccgcggc
      181 cggcgcccgc ccctgcgcat gcagcgagcg catgaggatc ctgccgggtc ggcgccagtc
      241 gtcgtgctcg tcgtctcggc cgggcgcctc caggccggcc agttggccgg gcggcccggt
      301 tcccagggcc gagacggacg ccgccccggc ggcgatactc gcccgccgtg atcccttcct
      361 cccgcgctgc ccatgtgcag ccacgccgcg tcgagccggc actgatcgtc atcgccgccg
      421 gcgtctgcgc cgccctgcat gtcggcaagt tgccgccggc catcgccacg ctgcgcgagg
      481 cgctgggaat gtctctggtg caggccggct tcctgctctc gctggtgcag gtcgcgggaa
      541 tgacctgcgg cgtcgccttc ggcgtcgtgg ccgacggcct cggccttcgg cgcagcatga
      601 tgctcggcct cgtcgtgctg gccgtggcga gcgcgctcgg cggcttttcg caaggggtcg
      661 ccatgttgat ggtgctgcgg gcgagcgaag gcttcggctt cctgctcgtc gtcctgccgg
      721 ccccggggct cgtccggcgc ctggtggcgc cgcaacgcgt cagcgccatg atgggtctgt
      781 ggggcgccta catgccgctg gcgacggcgc tggccctcct gatcgggccg ctctgcatcg
      841 gcgcctgggg ctggcgcgcg tggtggtggg cgctggccgc ggtgtccctg gcgatggcgg
      901 tagtgttgtg gcgctgcgtg ccggtgacgc tggcggcggc gcccgacgtg gccaccgcgc
      961 cgtggctgca gcgtctgcag cggacgctgg cggtgccggg cccgtggctg gtggcgctga
     1021 ccttcgccac ctattccggc cagtggctgg ccgtgatcgg cttcctgccc gccatctaca
     1081 cgcaggccgg cgtctcgggc gtggcgaccg gcctgctcac cgcgctcgcc gcggccgcca
     1141 acatcgtcgg caacgtcgcc gccgggcggc tgttgcagcg tggcgtggcg ccggcccgcc
     1201 tgctggtcat cggcttcgtg acgatggccg tcgcatccgc cgccacgttc gccggtgtcg
     1261 ccggcgacgg gctgccggcc tgggcacgct tcgtcgcggt gctgcttttc tcgggtgtcg
     1321 gcggcctgat cccggcgacg ctgttcgcgc tggcggtgcg cgtggcaccg gccgaaggga
     1381 cgctttccac gaccatcggc tggatgcagc agtggtcggc gctcggccag ttcttcgggc
     1441 cgccgctggt ggcctgggtg gcgagccgca ccggcggctg gcagttcacc tggctggcca
     1501 cgggcgcctg ctcggtcggc ggcctcgtgc tggccggtgc gctgacgcga gtcggcagat
     1561 tgcaaagggc ttgaggcgca tgacggcggc gccgacgatc gtcacgccct cgggcaggcc
     1621 gtcctgcaaa tagccgcgcg ccgggcgccc gggccggtct attcccgcgt gcccatccgc
     1681 aagagccgcg ccgtctcctc gtcgcgggca tccgccacga cgcgaagcac cccctgcacg
     1741 tcgaccggtc ggtgcccggg ctcgaagcgc ccggtcagcg gcgtggccgg ctgcaggacg
     1801 ccgcgcgtgt agagcgccca gatctcggga ccgtactgcg tggcgagcaa cgacgggtcg
     1861 aagcgcccga agaaatcgcg caggttggcg acatcgcgca gcagcatgcg ctgcgcgtgg
     1921 ttgttgcccg ccgcatcgac ggcctgcggc aggtcgatga tgaccggccc gtcggcggcc
     1981 agcaggatgt tgaactccga caggtcgccg tggatgacgc cggcgcacag catgcgcacc
     2041 acttctccca tcagggtggc gtggtgcgcg cgtgcttctt cggcgctgaa gtcgacgtcg
     2101 ttgaggcgcg gcgccgcgcc gccctcggcg tcgaccacca gctccatgag cagcacgcca
     2161 tcgtggaagt tgattggcct gggcacgcgc acgccggccg tcgcgagtcg atggagcgcg
     2221 tcgacctcgg cgctctgcca tgccgcttcc tgcgcctcgc ggccgaagcg cgtgcccttg
     2281 gccatggcgc gcgcctgccg ggtgttcttg accttgcggt tctcggtgta gtcgaccgcc
     2341 tggcgaaagc ttcgctgcgt cgcctccttg tagaccttgg cgcagcgcgt ctcgtcgccg
     2401 catcgcacga cgtagaccat cgcctccttg ccgctcatga gctggcggac gacggagtcg
     2461 atcagtcctt cctcgagcag cgggcgcagg cgctgcggcg tcttcacgcg cggcccgatc
     2521 gcgcggtgcc ggtgccggca tcgccgtcgg gatcggaaag ccacgcaggg acgtcccact
     2581 cctgcagctc ggccacgcgg cggacgaccc aggccgcttc ggccgcggcg aacgcctgcg
     2641 gatcggcgcg aagaccttcg tggcagcgcc gcaaggccgc actcgtgtcg tcgccttcct
     2701 gatggcgggc ggcgaggaac gccgccgcgt gcagcgccac gtcttcgcaa agcccgtagc
     2761 gggcctcgat cccggcccgc gagccgagcg cgcggccgtt gcgcgaatgg atcgcgagga
     2821 acgactcggg gacgagcgtc tggttgtggt cgtagaaatc gtgcatgggc gggcgcgcga
     2881 gcgtacaccc gcgcgaggtt atggcgacat gccctcctcg cgcgacggcc acgtcgctgc
     2941 ctgcgcttgg cgcccggcgg cgcggcgatc aggccttcgc ctgcatcgcg ggagagggct
     3001 cgcgcatcgt cgccgcgccg gtctcgagca ccgacttgag gttcgagagc accttcggcc
     3061 agccacccga caccgcctcg atcaccttcg cgtgctctcg ctcgcagctg tgcgtgacgg
     3121 cgagcttgca ggtgccgtcg tcggtcggct cgatctccat cgtgcagatc gtgtcgccct
     3181 cggccttcag ctcgggcttg atctggtgct gccagcgaat gacgaggcgg cgcggcggat
     3241 cggcctcgac gacgctgccc gagtcccaga cggcgccgtc cctggcgacg agcttccacg
     3301 gcgcgccggc gcgccactcg ctctcggagc gcatgccgaa ccagtactgg gtggtgaact
     3361 cggcgtcggt gagcgcgtgc cagagctttt gcggcgtggt gcggatgtag gtcacgtaga
     3421 cgaaggtcga tctagccatg aggtttctcc agtcgaagtt tgaggtccga gagtgccttc
     3481 aggcgcggct tctcgaactt ggcgatccag cgctcgtaca ggctttgcag aggcaccggg
     3541 ttgaggaaat gaagcttctc gcgaccctgt cgcacggtgg cgacgaggtt ggccgcctcg
     3601 agcacgccca ggtgctgcgt cacgccctgg cgcgtcatgt ccaggtgctc gcacagctcg
     3661 ttcagggtgc ggccgtcgct cgcgtgcagc aggtcgagga gcttgcgccg gctcgggtcg
     3721 gccagcgcct tgaagagtgc atcgtgatcg cgggcggctg ccatggttca taggcaagga
     3781 attgcttgcc tgaatgatag gcaagtcttt gcttgcctgt caagtggcgg cggctggcac
     3841 ggcgccgacg ccttccgccc gagggtcgtt gtccgctatc gtctcgccgt cgtctttcga
     3901 gtcggccatg atcaccaccc tcgtcaccct gctgcgcgac aagctcgtcg gtcgtgccga
     3961 cgcgctcgtc tcgggccacc cgctcgcccg cccgctgccg tcatggcgcg acgcgctcgc
     4021 ccgcatcggc cgcctggcgg tgctggccgg catcgccctc ctcgccacgg cctgcgcgac
     4081 gctgccgccg cccgcgcacc cggcgccgac gcaggccatc gccgactacg cgcagacacc
     4141 gctgggcgag atgacgcggg cggtcctgcc gaccgacggc cgctcgggct tcctgctcca
     4201 gccctacggc cccaacgcct tcgcaacgcg gctcgcgctg acgcggctgg ccacgcgcag
     4261 cctcgacgtg cagtactacc tgctgcaggg cgacaacgcc gggcgctcgc tgatgcgcgc
     4321 gctgcgcgac gcggccgcgc gcggcgtgcg cgttcgcgtg ctggtcgacg atctctacac
     4381 cgtcggcgaa gacgagctgc tgctcggcct ggccgcgtat cccaacgtcg aggtgcggct
     4441 cttcaatccg tttcccgccg gccgcgccga cgaggtcagc cgcttcatcg cctcggggct
     4501 cgacttcggc cgcgtcaacc ggcgcatgca caacaagctc ttcgtcgccg acaacgccgc
     4561 cgcggtggcc ggcggccgca acatggccga cgagtacgtg tcgaacacgc tcgggcgcaa
     4621 cttcatcgac atggacgtct tcgccgccgg cccggtggtg cgcgcgctct cggccgagtt
     4681 cgatcactac tggaacagcg atctcgtctt cgccgtcgat cgcatcgcca ccagcccgct
     4741 cggccgcgcc gagctgcagg cgcgcttcga gcgcgccacc cgcgatgcga cgccgcccga
     4801 ggccggcgcg gtcgcggccg acggcaaggt cgatcatccg atcgacggcg agccgacgtc
     4861 ggtttcgccc gacctggcgg ccatgctgaa cctgccgttc gagctggcga gccaccgcat
     4921 cgggcccttg ctgccggccg atgcgcgcgt gctgttcgac ccgctgacca agacccgggg
     4981 ctggaacgag cgcgaagact cgatcgcggg caccgtgacg gcgggcgtga tcggctggtt
     5041 tcgcacggcg cgggtcagca tcaagatggt gtcgccgtat ttcgtgccga acgacgccgc
     5101 cgtcgccagc ctctccgagg ccgtcgcctc cggcctcgac gtcgaggtcg tcaccaactc
     5161 gctcgcctcg accgacgagc cgctcgtcta cttcggctac tggccgcacg tcgccgcctt
     5221 gctgaaggcc ggcgtgaaga tccgcgagct gagcccatcg ctcagtgccg agcggcacag
     5281 gctcggcctg ttcggccggc gcaccggcgc gctgcacatg aagaacgcca tcgtcgacca
     5341 ccgccaggtc ttcctcggct cgatgaacct cgaccagcga tcggccaagc tcaacaccga
     5401 gctcggcctg atcatcgaca gcgccgagat ggcgcgccag ctcgaggcga tgaccgactc
     5461 gggcagcacc taccgcctgc gcctgaatgc cgggggcggc gtcgaatggg tcgccgacga
     5521 aggcgacggc aagacgcgcg tctacgacgc gccgccggag acgaccgcgt ggcagcgctt
     5581 caagacgcgg ctgctgagcg tcttcgttcc ggccggcgag ctgtgaagcc cggcggcgcc
     5641 gctaccgggc ggcgacgacg agctgccagt cggcgacaac gagctgccag tcggcgacaa
     5701 cgagctgcca gtcagcggcg acgagctacc agtcggcgac gacgagctgc ccgcccgtga
     5761 ggtagacgag cttcgagccg atctgcaggc tgcgctcgcc ccacaggctc gggtacgtca
     5821 tcgacccggc cggcgcgggc agcagcgact tcagcgccag gctttgcgcc tgcgtgtcga
     5881 cctcgatccg ctgcagcccg tgctgcgggc tcgggtcgcc gggcgccgcc gtgatgatca
     5941 tcggcaggcc gatccgcgcc acgttgccgc tctgcagcca gttgatgccg tggctcgacg
     6001 cgtcgagcgc ggtctggctg cccgaggcgc cgacgacgag cgtggcgagc gacttcggct
     6061 ggctggcgtc gctgacgtcg aacaggccga gctggatgcc gccgagacgg ttgctcgccg
     6121 ggtcgacgtc gcggcc
//
LOCUS       DASRWS010000271         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_110088, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000271 DASRWS010000000
VERSION     DASRWS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110088"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..787)
                     /gene="otnC"
                     /locus_tag="VFF72_04965"
     CDS             complement(8..787)
                     /gene="otnC"
                     /locus_tag="VFF72_04965"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: protein motif:HMM:NF006000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HZV92544.1"
                     /translation="MRRGRSRRNRSLNHNCNRNCNCNHNQDHDYSRSRSRSRNGSQQP
                     ETCCMSDARVREKLCLLGRSLFERGLTHGRTGNISVRTDRGFLMTPTGASLGGLDPAR
                     LSLLDGDGAHVGGDVPTKEALLHLAMYRARSGDGAVLHLHSGHAVAVSVLADVDPANA
                     IPPLTAYQAMRVGTLPLLPYFAPGDPALAGIVGVAARKHHALLLSNHGPIVSGSTLVV
                     AADVVEELEATARLWLQVRHEPVRCLTPGQLTELAERYPRN"
     gene            complement(828..2081)
                     /gene="otnK"
                     /locus_tag="VFF72_04970"
     CDS             complement(828..2081)
                     /gene="otnK"
                     /locus_tag="VFF72_04970"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HZV92545.1"
                     /translation="MLLGCIADDLTGAADLGVNLAREGLSVMQVNGVPDELALPEADA
                     VVVALKSRTIAADEAVAQSLSALEWLRSRGAERIYFKYCSTFDSTPRGNIGPVTEALQ
                     AALGGRIVASTPAYPRNARTVYRGHLFVGDVLLSESGMRQHPLTPMTDANLVRVLSAQ
                     CRRPVGLVSVADLEAGAAATRDKLAALETEGHGHAIVDATRDEHLAVAAEAALGLPLC
                     AGGAGLGLGLARRLAARARPGLSGWKAPTGAGPVAWLAGSCSQATLGQIAAVRDRVAS
                     FQIDPFELAADAGCARRVAERAVDALGAEPVLVYASAAAEALSKAQRALGVERAAGLV
                     EEAFREIAVALHAAGVRRFVIAGGETSGAVVGALKVRALVIGREIDPGVPWSLAVGGE
                     PLWLAMKSGNFGSPDFFVKATRMLR"
BASE COUNT          445 a          929 c          942 g          405 t
ORIGIN      
        1 ggactgctca gttcctcgga tagcgctcgg caagctcggt cagttgcccg ggcgtcaggc
       61 atcgcacagg ctcgtgtcgt acctgcagcc acagtctcgc cgtggcctcg agctcctcga
      121 cgacatcggc agcaaccacg agcgtgctgc cgctgacgat cgggccgtga ttggaaagca
      181 gcagcgcgtg gtgctttcgc gctgccacgc cgacgatccc ggcgagcgcg ggatcgcccg
      241 gcgcgaagta gggcaggagc ggaagcgtgc cgacgcgcat ggcctggtag gcggtgagcg
      301 gcggtatggc gttcgcaggg tcgacatcgg ccagaaccga caccgcaacg gcgtggccgg
      361 aatgcagatg aagcacggcc ccgtcgccgc tgcgggcccg gtacatggcc aagtgcagca
      421 gcgcctcctt ggtcggcacg tcgccgccca cgtgcgcacc gtcgccgtcc agcagcgaca
      481 gccgtgccgg gtcgaggcca ccgagcgagg cgcccgtcgg cgtcatcaga aagccgcggt
      541 cggtgcgcac agaaatattg ccggtgcggc cgtgcgtgag cccccgctcg aataacgacc
      601 gccccagcaa gcagagcttc tcgcgcactc tggcgtcgct catgcagcaa gtctccggct
      661 gttggctacc gttgcggctg cgactgcgac ttcggctgta atcgtggtcc tggttgtggt
      721 tgcagttgca gttgcggttg cagttgtggt tgaggctgcg gttgcgtcga ctgcgcccgc
      781 gccgcatgca gcgcgacgcg tccttcccga tgggcaatga gagatcctca tcgcaacatt
      841 ctggtcgctt tgacgaagaa gtcggggctt ccgaaattgc ccgacttcat ggccagccag
      901 agcggctcgc cgccgacggc aagggaccat ggcacgccgg gatcgatctc gcggccgatg
      961 acgagcgccc gcaccttcaa tgcgccgacc accgcgcccg atgtctcgcc gccggcgatg
     1021 acgaagcggc gcacgccggc cgcatgcagc gcgaccgcga tctcccggaa ggcttcctcc
     1081 accagcccgg ccgcacgctc gacgccgagc gcacgctggg ctttcgacag cgcctcggcc
     1141 gccgcgctcg catagaccag caccggctcg gcaccgagcg cgtcgaccgc gcgttcggcc
     1201 acgcgccggg cgcagccggc gtcggcagcg agctcgaaag ggtcgatctg aaacgaggcg
     1261 acgcggtcgc gaaccgcggc gatctgcccc agggtggctt gcgaacaact gccggcgagc
     1321 caagcgacgg gaccggcacc ggtcggcgcc ttccatccgc tcaggcccgg gcgggcgcgg
     1381 gccgccagac ggcgcgccag tccgaggccg agcccggcac ccccggcgca cagcggcagg
     1441 ccgagtgccg cctcggcggc gacagcgaga tgctcgtcac gcgtggcatc gacgatcgcg
     1501 tgaccgtgcc cttcggtctc cagcgccgcc agcttgtcgc gcgtggcagc cgcaccggct
     1561 tcgagatcgg cgacgctgac gaggccgacc ggacgccggc actgtgccga tagcacgcgc
     1621 accaggttcg cgtccgtcat cggcgtcagc ggatgctggc gcatgccgga ttcgctcagc
     1681 agcacgtcgc cgacgaagag gtgtccccgg tagacggttc gggcgttcct cggatacgcg
     1741 ggcgtcgagg cgacgatgcg gccgccgagc gcggcctgca aggcctcggt cacggggccg
     1801 atgttgccgc gtggcgtcga atcgaaggtc gagcagtact tgaagtagat gcgttcggcg
     1861 ccgcggctgc gcaaccattc gagcgccgaa agcgactgcg ccaccgcctc gtcggcggcg
     1921 atggtgcgcg acttgagcgc gacgacgacc gcatcggcct cgggcagcgc gagctcgtcg
     1981 gggacgccat tgacctgcat caccgacagg ccttcgcgcg cgaggttgac gccgagatcg
     2041 gcggcgcccg tcaggtcgtc ggcaatgcag ccaagcagca tcgtggcctc aggcgaccaa
     2101 cacaagcgcg cagccggcct cgagcgcctg ccgggcccga gcccctgccc ggatgccggt
     2161 cacggcctgc ccgatcggcg cattcggaaa acacgatagt tgcatgcgaa caattatcga
     2221 ccggcgctca ccaagcgacg ccaattccaa ttggcaagac cggcgccaat gcagaaacgc
     2281 gatgtcgaca acgtgccgac ggcaggttgc gcacgggatt cgacgctcgt cgcgctcgcc
     2341 gtctgcgcca tcacgatacc tcgtcgagat ctccggcctg cttcatcgga tggccggaca
     2401 ccggcgtcga ctagcaggaa cccgattgac agcgacgaag ggccggcgca gcatcaagtt
     2461 cgcatcgatg catggtggtc caggccatcg cagtcctctc gtctgtccgg ggtcgcaagt
     2521 gggagtttcg ttgagcgaag tggcgctgag gctcgagatc tcgttgccgc tgttttccgc
     2581 gtgctgagca gcagcggctg cgctgtcgaa acgtgaccgg gatggagtcc gccgcaggtc
     2641 ggtgcgtcga ggttcgtggc gtatcgaagc aattcgagag ccgggccggg cggcagacgg
     2701 ccatctccgc agtcgatctg a
//
LOCUS       DASRWS010000272         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_110273, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000272 DASRWS010000000
VERSION     DASRWS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110273"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="ydiK"
                     /locus_tag="VFF72_04975"
     CDS             complement(<1..917)
                     /gene="ydiK"
                     /locus_tag="VFF72_04975"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HZV92546.1"
                     /translation="MPRRDLAQTLLSMVCLGALIVAAFWIVRPFIAATIWATTIVVVT
                     WPLLLRIEKRLWNSRWLSITAMLVILLILFMVPLALLVGTLLSNADQIAFEFNSLLAF
                     RMPTPPDWLVGLPVVGARLASLWHDTAAAGAEGLWARLAPYAGTVTGWLVARAGGVGY
                     LSLQYLLTLILAAFIYANGEEAAFAAKRLGRRLGGRSGEDLVHLAGQATRAVAIGVGV
                     TALVQSILGGIGLALAGVPFALLLTAVLFMLCIAQIGMLVVLIPAVAWVYWSGHPWIG
                     TVLLVWSLVVSLIDNVLRPLLVRRSADVP"
     gene            complement(1119..1892)
                     /locus_tag="VFF72_04980"
     CDS             complement(1119..1892)
                     /locus_tag="VFF72_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZV92547.1"
                     /translation="MTIETDGFRKGKTAMNFPPGETALATRPRGDLDRGPACQAGDED
                     PARNPSARLESWLAATLDEVDYGVVVLASADGIRFANRVAMAELRAAVALRAVDGRLR
                     SVRGEDAGPLERALADARQRSLRRMLIVGHGQERLGLSVMPLGTAREEAAGTVVLIGK
                     REVCPLSIAGYSRSHGLTDAEGRVLAELCRGARPQDVARRHAVSIATVRTQIGSIRAK
                     TGATSIVALLRQLASLPPMLVALGRVEASPLRGLARSSI"
     gene            2262..>2572
                     /locus_tag="VFF72_04985"
     CDS             2262..>2572
                     /locus_tag="VFF72_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZV92548.1"
                     /translation="MDENRLPAPLLEALVRHGQARSFRANTVLIHEGDAGDALYIVLD
                     GKARAFASSPDGRDVVLGDYGPGEYFGELALDGGPRSASVKTLVATSCRVVQGAELRP
                     FL"
BASE COUNT          441 a          875 c          908 g          348 t
ORIGIN      
        1 aagggtacgt cggcgctgcg gcgcacgagc agcggccgca ggacgttgtc gatcaggctg
       61 acgaccagcg accagacaag cagcaccgtg ccgatccacg gatggccgct ccagtagacc
      121 caggccaccg ccgggatgag cacgaccagc atgccgatct gcgcgatgca gagcatgaag
      181 aggacggcgg tgagaaggag cgcgaacggc acgccggcca gggccagccc gatgccgccg
      241 agaatcgact ggaccagcgc cgtcacgccg acgccgatcg cgacggcccg cgtcgcttgc
      301 ccggcaagat gaacgaggtc ctcgccgctg cgtccgccga ggcggcggcc cagccgcttc
      361 gcggcgaacg cggcttcctc gccattggcg tagatgaagg cggccaggat cagtgtcagg
      421 aggtattgca ggctcaggta gccgacgccg ccggcgcgcg cgaccagcca tcccgtcacc
      481 gtccccgcgt agggggcgag ccgggcccag agcccttcgg cgcccgccgc cgccgtgtcg
      541 tgccagaggc tcgccagtcg agcgccgaca accggcaggc cgacgagcca atcgggtggc
      601 gtcggcatgc gaaaggcgag cagggaattg aactcgaagg cgatctggtc ggcattcgag
      661 aggagcgttc cgaccagcag cgccagcggg accatgaaca ggatcagcag gatcacgagc
      721 atcgccgtga tcgacagcca gcggctgttc cacaggcgct tctcgattcg cagcagcagc
      781 ggccaggtga cgacgacgat cgtcgttgcc cagatcgtcg cggcgatgaa gggccggacg
      841 atccagaacg cggcgacgat gagcgcaccg aggcagacca tgctgagcaa ggtctgcgca
      901 aggtcgcgac gaggcatggg cactggcgag tggaagaagg cgccacgcgg tgtcgcgcgg
      961 tgcgcaaggc tacccgatgc cgggccgcgg gcgtccgtcc tggcgtcgac gctcgaaatg
     1021 cgtcgcttcg ccccgcatcg gcgagcgcgc tttggtgaga cgccgtattc cggcggagag
     1081 cggcgcctgt tcgaaggctg tcgaagcggc gtcacggatc agatggaaga ccgcgccagg
     1141 ccgcgcagcg gcgacgcctc gacgcgaccg agcgcgacca gcatcggcgg tagcgacgcg
     1201 agctggcgaa gcagggcgac gatgctggtc gcgccggtct tggcgcgaat gctgccgatc
     1261 tgggttcgca cggtggcgat cgataccgca tggcggcgtg ccacgtcctg cggacgggcg
     1321 ccacggcaca gctcggcaag cacgcggcct tccgcgtcgg tcaacccgtg cgagcgcgag
     1381 tagccggcga tcgacaacgg gcagacttcg cgcttgccga tcaggacgac cgtgcctgcg
     1441 gcctcctcgc gagccgtgcc gaggggcatg accgacagtc cgagcctttc ctggccgtgg
     1501 ccgacgatca gcatgcgacg caggcttcgc tgccgcgcat cggccagggc ccgctccagg
     1561 gggcccgcat cttcgccgcg gacgctgcgc aaccggccgt cgacggcccg cagcgcgacg
     1621 gcggcgcgaa gttccgccat ggcgacgcga ttcgcgaagc ggatcccgtc ggccgatgcc
     1681 aggacgacga cgccgtagtc gacttcgtcc agtgtcgcgg ccagccacga ctcgagccgt
     1741 gccgaagggt ttcgtgcggg atcttcgtcg ccagcctggc acgccggtcc tcggtcgagg
     1801 tcacctcgcg gccgggtggc gagggccgtt tcacccggag gaaagttcat ggcggtcttg
     1861 cctttcctga agccgtcggt ttcgatcgtc acacctgcac gccggcgtca gccccacgtg
     1921 ccgcgctcga tgcaagggtg cggggcgggc gaggagtgcc gggcccgacc cgactcaaat
     1981 ctggcccgtc accgcggcga agatcgccga aacggcggca aacccgtaga ccaggaccac
     2041 gacggcggta gcgacgtcat cgatgagcgt tttcatgtcg attcccttct gcgttgcgcc
     2101 atcgcgacct cgcgacgact gatcggcagt ctcgaagaac gacgaccgtt attgaaggca
     2161 gcaaggcaca gccggcgagt gacttgcgtc acaccgctgt ttgacgctcc aatcccggct
     2221 ggtgacgaaa gaccgggggc cacgccccaa aaggagcagc gatggacgag aaccgactac
     2281 ccgcgccgct gctcgaggcg ctcgtgcggc acggccaggc gcggtcgttt cgcgccaaca
     2341 ccgttctcat ccacgaaggc gacgcgggcg acgccctcta tatcgtgctc gacggcaagg
     2401 cacgcgcctt cgccagctcg cccgacgggc gcgatgtcgt cctcggcgac tacggtccgg
     2461 gcgaatactt cggcgagctc gccctcgacg gcggcccgcg ctcggcgtcg gtgaagacgc
     2521 tggtggccac ctcgtgccgc gtcgtccagg gggccgagtt gcgcccgttc ct
//
LOCUS       DASRWS010000273         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2128616, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000273 DASRWS010000000
VERSION     DASRWS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128616"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VFF72_04990"
     CDS             <1..1069
                     /locus_tag="VFF72_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZV92549.1"
                     /translation="RECCVKSLARSQLAHWTPDLETDDVPELVEQIRTRLLPRRGVFA
                     LREEIILTVGTQHAYFLLAEALFDEQTRLGLEEPGHPHARNSFALRGPRAVGIGVDAE
                     GLVVDALPPLDYVYVTPSHQSPTTATLSLERRQRLLHKAELQDFVLIEDDYEAENLYA
                     GTPMPALKSLDKSGRVIYIGSVSKNLSPALRLGYIVAPRALVQELRLLRHAMVRHPSA
                     FLQHAYALFLSLGHHESHARRVNQAMQERLAVAAEALRDELPDFDFILPQGGASLWVS
                     APEWVESADLAAMARGRGVLIEAGAVFFTRPPGPCPFFRLRFSSIGATQIRDGIHALA
                     LAVADLAAARGHERPATRLTH"
     gene            1319..>2315
                     /gene="tauA"
                     /locus_tag="VFF72_04995"
     CDS             1319..>2315
                     /gene="tauA"
                     /locus_tag="VFF72_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV92550.1"
                     /translation="MKLNLSRRDFNLTAANLLAGSVAGTSLLGAGNAFAQQKSVTVGY
                     QLIVGPFLVAIANGSFEKATGYKIDWRQFSSAGDITSAFASGDVPVGVLGSTGIAAAA
                     TRGVDMELFWILDNIGKSEALVARNGSGINKPSDLKGKKVAVPFVSTSHFHLLVALEQ
                     IWHIKPSDVQILNMKPPQIVAAWARGDIDAAYVWPPALTEIEKTGKTIITSEEVGKKS
                     VPTFDGLVASKSWAAKNEDFMVKFTKVLEQTYADYRANSAKWTADSPEVKAMVKLVGG
                     KAEDDADSLKLLVYPTAKEQASAEWLGGGKNGGALRALTKSAEFLKAQKQIDHVLP"
BASE COUNT          389 a          841 c          751 g          334 t
ORIGIN      
        1 ccgcgagtgc tgcgtcaaga gcctcgcccg ctcgcagctc gcgcactgga cgcccgacct
       61 cgagaccgac gacgtgcccg agctcgtcga gcagatccgg acccggctcc tgccgcggcg
      121 cggcgtcttc gcgttgcgcg aggagatcat cctcaccgtc ggcacgcagc acgcctattt
      181 cctgctcgcc gaggcgctgt tcgacgagca gacgcggctc ggcctcgagg agcccggcca
      241 tccgcacgcc cgaaacagct tcgccctgcg cgggccgcgc gcggtcggca tcggcgtcga
      301 tgccgaaggc ctcgtcgtcg atgcgctgcc gccgctcgac tatgtctacg tcacgccttc
      361 gcaccagagc ccgaccaccg cgacgctctc gctcgaacgc cggcagcggc tgctgcacaa
      421 ggccgagctg caggacttcg tgctgatcga ggacgactac gaggccgaga acctctacgc
      481 cggcacgccg atgccggcgc tgaagagcct cgacaagagc ggccgcgtga tctacatcgg
      541 ctcggtctcg aagaacctct cgccggcgct gcgcctgggc tacatcgtcg cgccgcgggc
      601 gctggtgcag gagctgcgcc tgctgcgcca cgcgatggtg cgccatccga gcgcattcct
      661 gcagcacgcc tacgccctct ttctctcgct cggccatcac gagtcgcacg cgcggcgggt
      721 caaccaggcg atgcaggaac ggctcgccgt cgccgccgaa gcgctgcgcg acgagctgcc
      781 cgacttcgac ttcatcctgc cgcagggagg tgcctcgctc tgggtgagtg cgccggagtg
      841 ggtcgagagc gccgatctcg cggcgatggc gcgcggccgc ggcgtgctga tcgaggccgg
      901 cgccgtcttc ttcacccggc ctccgggccc gtgcccgttc ttcaggctgc gcttttcatc
      961 gatcggcgca acccagatcc gcgacggcat ccacgccctg gccctggccg tcgccgacct
     1021 ggcggcggcg cgcggacacg agcgtccggc gacacggctg acccattgag gcgagccgct
     1081 gtcgcgcgac acggcccgac cctgttgcag cggcacaggg taagtcccga gggactggct
     1141 ccattcggca ccgagcgctg gcccttaccg gcggcgcgcg gcgcgcacac actcggctcc
     1201 gtcccataag atgctccgca aggtggctgg ctgggcggcg gcaagcccgc tgactcgacg
     1261 acacattgcc gcgcgccgcc ggcgaccagc gatccgaaac ccaaaaccga ggtccaccat
     1321 gaaactgaat ctctcgcgac gcgatttcaa cctgaccgct gccaacctgc tggcgggctc
     1381 ggtcgccggc acatcgctgc tgggggccgg caacgccttc gcgcagcaaa agagcgtcac
     1441 ggtcggctac cagctgatcg tcggcccctt cctcgtggcg atcgccaacg gcagcttcga
     1501 gaaggccacc ggctacaaga tcgactggcg ccagttttcc tcggcgggcg acatcacgag
     1561 cgccttcgca tcgggcgacg tgccggtcgg cgtgctcggc tcgaccggca tcgccgcggc
     1621 ggcgacgcgc ggcgtcgaca tggagctgtt ctggatcctc gacaacatcg gcaagtccga
     1681 agcgctcgtc gcgcgcaacg gctcgggcat caacaagcca agcgacctga agggcaagaa
     1741 ggtcgccgtc ccgttcgtct cgacctcgca cttccacctc ctcgtcgcac tcgagcagat
     1801 ctggcacatc aagccgagcg acgtgcagat cctcaacatg aagccgccgc agatcgtcgc
     1861 cgcgtgggcg cgcggcgaca tcgatgccgc ctacgtgtgg ccgccggcgc tcaccgagat
     1921 cgagaagacc ggcaagacca tcatcacgtc cgaggaagtc ggcaagaaga gcgtgccgac
     1981 cttcgacggc ctcgtcgcca gcaagtcgtg ggcggccaag aacgaggact tcatggtcaa
     2041 gttcaccaag gtcctcgagc agacctacgc cgactatcgg gccaacagcg ccaagtggac
     2101 cgccgactcg cccgaggtca aggcgatggt caagctcgtc ggcggcaagg ccgaggacga
     2161 cgccgattcg ctcaagctgc tcgtctatcc gaccgccaag gagcaggctt cggccgaatg
     2221 gctgggcggc ggcaagaacg gcggcgcgct cagggcgctc accaagagcg ccgaattcct
     2281 gaaggcgcag aagcagatcg accacgtgct gcccg
//
LOCUS       DASRWS010000274         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2692415, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000274 DASRWS010000000
VERSION     DASRWS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692415"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFF72_05000"
     CDS             <1..809
                     /locus_tag="VFF72_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZV92551.1"
                     /translation="CNRKRDVATLHTTLKKHGINAGALHGDMSQPARTETLEKFKAGD
                     IQILVASDVAARGLDVQDMSHVFNYDVPFSPEDYVHRIGRTGRAGKTGHSFTLATPNE
                     GDLIEAIQKLINTVIPRVEVPGLDIVTLEAGDGRRRGRGGRRGGRSSGGAPERGARGP
                     RREPREPRAERPAETVQAEAQPAATQAATESQPEARAPREPRPPREPRPDRPPRERRP
                     EQRGESRGERRPEQRSEHRSERRPERHHDRDEGNSPVGLGDHVPAFLARR"
     gene            834..1055
                     /locus_tag="VFF72_05005"
     CDS             834..1055
                     /locus_tag="VFF72_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZV92552.1"
                     /translation="MVKCELGKAYEVADDAVTVEQVSEVYSTSGQYDLLMKCYLDEKT
                     DIGHFVTSRIQTLAGVKDTFTLITFKAFT"
     gene            1169..2188
                     /locus_tag="VFF72_05010"
     CDS             1169..2188
                     /locus_tag="VFF72_05010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZV92553.1"
                     /translation="MRAGPVPSVLPRGEEIAPRYVADGLEHDVAAFMDRNAIVGVLVI
                     RRGKVVLERYGLGLGEHDRWSTMSTVKSMTSMLVGAAVHDGAIASIDDPLTRYLPALR
                     NSSYEGVSLRHVMTMSSGVRWTEDYGDRTSDVNRYSKSLADKVPGGVLRLMASLPRAR
                     PPGTTFLYNSGDTYLLGAALTAAIGKPLADYMSEKVWRPAGMECDAFYTVESEGGQEI
                     GGSRAGVVLRDFGRFALFVAADGVIGGKRVVPEGWVAASATPAFALAASPVADITHYG
                     YSWWLGDGVMAALGHAGQRIDIFRDEGLVVVTLGAFPEPQYVLPGAHDRRNEVVAFTR
                     AVRAT"
     gene            2185..>2525
                     /locus_tag="VFF72_05015"
     CDS             2185..>2525
                     /locus_tag="VFF72_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92554.1"
                     /translation="MIGGRAALAIWVDIDPADDAQFEHWHSREHVQERVNCPGWLRGS
                     RFKGVETSGRYLLLYDAEATSAFESEEYYARLRAPTQMSRAIFPKFRDTWRTVCRVEQ
                     RWGDGVGAAVLT"
BASE COUNT          384 a          915 c          858 g          368 t
ORIGIN      
        1 tctgcaaccg caagcgcgac gtcgcgaccc tgcacacgac gctgaagaag cacggcatca
       61 acgccggcgc cctgcacggc gacatgagcc agcccgcgcg caccgagacg ctcgagaagt
      121 tcaaggccgg cgacatccag atcctcgtcg cctccgacgt ggccgcgcgc ggcctcgacg
      181 ttcaggacat gagccacgtg ttcaactacg acgtgccgtt ctcgcctgag gactacgttc
      241 atcgcatcgg ccgcaccggc cgcgccggca agaccggtca ctccttcacc ctggcgacgc
      301 ccaacgaagg cgatctcatc gaggcgatcc agaagctgat caacaccgtc atcccgcggg
      361 tcgaagtgcc gggcctggac atcgtcacgc tcgaggccgg cgacggccgt cgtcgcggtc
      421 gcggcggccg ccggggcggt cgcagcagtg gcggcgcacc ggaacgtggt gcccgcggcc
      481 cgcgccgcga gccgcgcgag ccccgggccg aacggccggc ggagacggta caggccgagg
      541 cccagcctgc cgccacccag gccgccaccg agtcccagcc tgaagcccgc gcgccgcgcg
      601 aaccgcgtcc gccgcgcgag ccgcgccccg accgtccgcc gcgcgagcgc cggccggaac
      661 agcgcggcga gtcccgcggc gaacgccgtc ccgagcagag gtccgagcat cgttcggagc
      721 gccgcccgga gcgtcatcac gatcgtgacg agggcaactc gcccgtcggc ctcggcgatc
      781 acgtgccggc cttcctggcc cgccgctagg gcgcgatgca gacgatcttc gtcatggtga
      841 agtgcgaact cggcaaggcc tacgaggtcg ccgacgacgc ggtcacggtc gagcaggtgt
      901 cggaggtata ctcgacctcc ggccagtacg acctcctgat gaagtgctat ctcgacgaga
      961 agaccgacat cggccatttc gtcaccagca ggatccagac ccttgccggc gtgaaggaca
     1021 ccttcaccct catcaccttc aaggccttca cctgagcgtt tcggcgcctc gccagcatct
     1081 gccgcgcggc gaggacttcc tgctcctgcc gccgtcgatc cagccctacg cctatcgcat
     1141 cgtcgacaga ctgttcgcgc accgcgtcgt gcgcgctgga ccggtgccca gcgtgctgcc
     1201 gcgcggcgag gagatcgcgc cacgctacgt ggccgacggc ctcgagcacg acgtcgccgc
     1261 gttcatggac cgcaatgcca tcgtcggcgt gctggtgatc cgccgcggca aggtggtgct
     1321 ggagcgctac ggcctcggcc tcggcgagca cgaccgctgg tccaccatgt cgacggtgaa
     1381 gtcgatgacc tccatgctgg tcggtgcggc ggtccacgac ggcgccattg cctcgatcga
     1441 cgatccgctg acgcgctacc tgccggccct gcgcaattcc tcgtacgagg gcgtcagcct
     1501 gcggcacgtc atgaccatgt cgtcgggtgt gcgctggacc gaggactacg gcgaccgcac
     1561 ctccgacgtg aaccgctaca gcaagtcgct ggccgacaag gtgccgggcg gcgtgctgcg
     1621 cctgatggca tcgctgccgc gcgcccggcc gccgggcacc actttcctct acaattcggg
     1681 cgacacctat ctgctgggtg cggccttgac cgccgcgatc ggcaagccac tggcagacta
     1741 catgtcggag aaggtctggc ggccggccgg catggagtgc gacgccttct ataccgtcga
     1801 gagcgagggc ggccaggaga tcggcggcag ccgcgccggc gtcgtcctgc gcgacttcgg
     1861 ccgcttcgcg ctgttcgtcg ccgccgacgg cgtgatcggc ggcaagcgcg tcgtgccgga
     1921 gggctgggtc gcggcgtcgg cgacgccggc gtttgcgctc gcggcctctc cggtcgccga
     1981 catcacgcat tatggctaca gctggtggct cggcgacggc gtgatggcgg ccctcggcca
     2041 tgccggccag agaatcgaca tcttccgcga cgaaggcctg gtggtggtga ccttgggtgc
     2101 gttccccgag ccccagtacg tgctgcccgg cgcgcacgat cgacgcaacg aggtcgtggc
     2161 cttcacccgc gccgtgcgcg ccacgtgatc ggcgggcggg cggctctcgc gatctgggtc
     2221 gacatcgatc cggccgacga cgcccagttc gagcactggc acagccgcga gcatgtccag
     2281 gagcgggtga actgccccgg atggctgcgc ggcagccgct tcaagggcgt cgagacctcc
     2341 gggcgctatc tcctgctcta cgacgctgag gcgacgtcgg ccttcgagag cgaggaatat
     2401 tacgcgcgcc tgcgcgctcc gacgcagatg agccgcgcga tcttcccgaa gttccgcgac
     2461 acctggcgca ccgtttgccg ggtcgagcag cgctggggcg acggcgtcgg cgcggcggtg
     2521 ctcac
//
LOCUS       DASRWS010000275         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3287316, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000275 DASRWS010000000
VERSION     DASRWS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3287316"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFF72_05020"
     CDS             complement(<1..360)
                     /locus_tag="VFF72_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92555.1"
                     /translation="MLARLRRVADGQGVEVLAPALHKYLLPCPPRERDSRFEHMRQHV
                     TIAPLLQNGAVAGLIVTIEDVTARFDRERRLSADLDSSDESVRWRAVQALAAGGESPA
                     LLSSALTDQSWRIRRAAA"
     gene            complement(516..1976)
                     /locus_tag="VFF72_05025"
     CDS             complement(516..1976)
                     /locus_tag="VFF72_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZV92556.1"
                     /translation="MAASIEQVAASAENMTGLIEEVATSIEETSRSVAGVAQSAETIT
                     SSVESAATSATQLERSIRGVTQLVSEASDLAQRVTRDAHEGGQAIDRSIEGLARVRES
                     IGQSASVVKEVGKRTSDITSIVDTINLIAERTNLLSLNASIEAARAGEAGRGFAVVAE
                     EIRNLADRSAKATADIASIIKSLQNVVQDAVSSSNDVQRVAEESGRLAADGAAGLQRI
                     LGSIAQSSTKVAQIARATDEQLVAGQSLSAAIASAATQSRQVSAGTGEQARALQSLVR
                     TTSQMRSVAQQVSQAMNDQGQSSREIIKAGQNGAALASQVRRAMNEQSTAADQITKSM
                     DSIRRGASATARALAEQAAAVTEVTAETENLTRMIGSVSSGMAEQATAMSQISAATDA
                     VRRQTDQTTRGISEQARAAADLTTATRNVSTQMVLVTRANTEQSQAVAALSRDLLEAQ
                     RLGRATVQGAEATGALSQQLADRARALGEISAGSHS"
BASE COUNT          275 a          730 c          722 g          374 t
ORIGIN      
        1 cgccgcggcg cggcggattc gccagctctg atcggtgagt gcgctcgaca gcagcgcagg
       61 agactcgcca cccgccgcga gcgcctgcac ggcacgccac cgcaccgatt catccgagct
      121 gtcgagatcc gccgagagcc ggcgctcgcg gtcgaacctg gccgtgacgt cctcgatggt
      181 gacgatcagg ccggccaccg cgccgttctg gagcagcggc gcgatggtga catgctgccg
      241 catgtgctcg aaccgcgagt cgcgctcgcg cggtgggcag ggcagcaggt acttgtgcag
      301 cgcgggcgcg agcacttcga cgccctgacc gtcggcgacg cggcgcagcc gggcgagcat
      361 tccacgttcg gcgagaccag gatagaggcg ggacagcggc tcaccgcacg cggcggtttc
      421 cggaacgccg gtcgcctcgg cgagccacgg atcccacgtc tgaatgacga gcgcgcgatc
      481 cgtcgtgaac atgccgacgg acttgcgtcg gcgtgtcacg aatggcttcc ggctgagatt
      541 tcgccgaggg cgcgggcgcg atcggcgagc tgctggctga gcgcgccggt tgcctcggcg
      601 ccctgcaccg ttgcccggcc aaggcgctgc gcctcgagca gatccctgct gagcgcggcc
      661 accgcctgcg actgctcggt gttcgcgcgc gtgacgagca ccatttgcgt cgagacgttg
      721 cgcgtcgccg tcgtgagatc ggctgccgcg cgggcctgct ccgaaattcc cctggtcgtc
      781 tggtcggttt gccgccggac ggcgtcggtg gccgcggaga tctgcgacat cgccgtggcc
      841 tgctccgcca tgcccgagct gacgctgccg atcatcctgg tgaggttctc ggtttccgcg
      901 gtgacttcgg taacagcggc cgcctgctcg gcgagcgcgc gcgccgtggc actcgcgccg
      961 cgccggatgg agtccatcga ctttgtgatc tggtcggcgg cggtgctctg ctcgttcatg
     1021 gcgcggcgca cctgcgaggc gagcgccgct ccattctggc cggccttgat gatctcgcgc
     1081 gacgactggc cctgatcgtt catcgcctga cttacctgct gcgccaccga tcgcatttgc
     1141 gaggtggtgc ggaccagact ctgaagcgcg cgcgcctgtt cgcccgtgcc ggccgacacc
     1201 tgccgcgatt gggtggccgc ggaggcgatg gccgccgaca agctctggcc ggcgacgagc
     1261 tgctcgtcag tggcgcgggc gatctgcgcc accttggtcg agctctgtgc gatgctgccg
     1321 aggattcgct gcaagcccgc ggcgccgtcg gcggcgaggc gcccgctctc ttcggcgaca
     1381 cgctgcacgt cattcgacga cgacacggcg tcctgtacca cgttctgcag cgacttgatg
     1441 atcgaggcga tgtccgccgt cgccttggcc gaccggtcgg cgaggttgcg gatctcttcg
     1501 gcaacgacgg caaagccgcg ccccgcttcg ccagcacgcg ccgcttcgat cgacgcgttc
     1561 agcgacagca ggttcgttcg ctcggcgatc aggttgatcg tgtcgacgat cgacgtgatg
     1621 tcgctggttc gcttgccgac ttccttgacg accgacgccg attggccgat ggattcgcga
     1681 accctcgcga gcccctcgat cgatctatcg atggcctgac caccttcgtg cgcatcgcgg
     1741 gtcacgcgct gggcgagatc gctggcctcg ctcacgagct gcgtcactcc gcgaatggag
     1801 cgctcgagct gcgtggcgct ggtggccgcg ctctcgaccg acgacgtgat cgtctcggca
     1861 ctctgcgcca cgccggcgac cgagcgcgac gtctcttcga tcgatgttgc cacttcttcg
     1921 atgaggcccg tcatgttctc ggcgctggcg gcgacctgct cgatcgatgc agccatgctg
     1981 ttgatcgagc cggacgtttc ctccgacgcg ctcgccagct cgtcggcatt cgcacggacg
     2041 ccgtcgatcg agcgggccat ctgttcggcg gcggcggcca cctcgctcac ggatctggcc
     2101 a
//
LOCUS       DASRWS010000276         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2692929, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000276 DASRWS010000000
VERSION     DASRWS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692929"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFF72_05030"
     CDS             <1..619
                     /locus_tag="VFF72_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZV92557.1"
                     /translation="LMLKGLTVQYLFRQTFPLKGGETILFHAAAGGIGLIACQWARAL
                     GLKLIGTAGSDAKCALARANGAAFTINYRTEDFAARVKEITGGAGVKVVYDSIGRDTW
                     DKSLDCLRPLGLMASFGNASGVVPPFAPGILGAKGSLYVTRQTLFTHIATREAMQSMS
                     DDLFAAVTAGQVKIHVEQRYALADAAQAHRDLEARKTTGSSVLIP"
     gene            630..1262
                     /locus_tag="VFF72_05035"
     CDS             630..1262
                     /locus_tag="VFF72_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZV92558.1"
                     /translation="MTTLYGTKGSGSAAVEAALEVIGLPFRQVEAASWQPSAGLDELR
                     RVNPLAQIPTLVLDDGSVLNESAAILIDLGLAHPQSGLLATEPAPRAQQIRGLVYIAA
                     NCYAGIGILDYPDRWYPDPDDAVKAKMKERGRARLHELWDLFADQFPASPWLGGPRLG
                     ALDILAATVSRWSGTRQHLQASRPAFSELLARIDAEPRIAAVWARHWPKP"
     gene            complement(1272..1757)
                     /gene="lspA"
                     /locus_tag="VFF72_05040"
     CDS             complement(1272..1757)
                     /gene="lspA"
                     /locus_tag="VFF72_05040"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZV92559.1"
                     /translation="MAARRPSIWPWLAVSLVVILVDQLTKTIIQRTFSANESHSVNAF
                     LDIVRAHNTGAAFSFLAGASGWQRWFFIGLGAVAAVFIVFLLARHGGQRLFRWALALI
                     LGGAIGNVIDRLWHGWVVDFIQVHWRGAYFPSFNVADSAITIGACLLVLDELLRVRRS
                     R"
     gene            complement(1760..>3431)
                     /locus_tag="VFF72_05045"
     CDS             complement(1760..>3431)
                     /locus_tag="VFF72_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZV92560.1"
                     /translation="PVIYRAAAQWFIRMDRPDASTAGVFAVDPAPDALRETALAAIGD
                     TAFYPENGRARLHDMIAHRPDWCISRQRNWGVPLPFFLHKVTGELHPDTLALVDRAAA
                     IVEAGGVEAWSRLGADDVIGATGPHSAEHYAKSNDILDVWFDSGSTFFHVLRGSHPGT
                     TIADDDGARPEADLYLEGHDQHRGWFHSSLLIACALEGHAPYRGLLTHGFTVDSEGRK
                     MSKSLGNFVGLHETTEKLGAEIIRLWCASTDYSGDLAITPNILARVVDAYRRIRNTLR
                     FLLANTSDFDAAKDAVPHADMLEVDRWALARAAALQAEIVGDFDTASGLFRGGHYGVY
                     EFHPVVAKLQVFCSEDLGAFYLDVLKDRLYTTAPASRARRSAQTALAQIAQAMLRWMA
                     PFLSFTAEEAWKTIGPRDSVSIFAETYSDTRAWRDDALLAKWTRIGEVRAESNRQIEA
                     LRADGKLGSSLQAEVTIKAGPTRLAELATLGDDLRFVLITSAARLESAGDTADPGEFF
                     VHAAVSDAAKCERCWHYRTDVGIDPAHPTICGRCTSNLFGAGETRTVA"
BASE COUNT          540 a         1158 c         1230 g          503 t
ORIGIN      
        1 gctgatgctc aagggcctca ccgtgcaata cctgtttcgg cagacgttcc cgctcaaggg
       61 cggcgagacg atcctgttcc atgccgccgc gggcggtatc ggcctgatcg cctgccaatg
      121 ggcgcgcgcg ctcggactga agctgatcgg cacggcgggc agcgacgcga agtgcgccct
      181 ggcgcgcgcc aacggcgcag ccttcacgat caactaccgg accgaggatt tcgcggcccg
      241 cgtcaaggaa atcaccggcg gcgccggcgt caaggtcgtc tacgactcga tcggcaggga
      301 cacctgggac aagtcgctcg actgcctgcg tccgctcggc ctgatggcga gcttcggcaa
      361 cgcctcgggc gtcgtcccgc cgttcgcgcc gggcatcctc ggcgccaagg gctcgctcta
      421 cgtgacgcgg cagacgctct tcacgcacat cgccacgcgc gaggcgatgc agtcgatgtc
      481 cgacgacctg ttcgccgcgg tcaccgccgg acaggtgaag atccacgtcg agcaacgcta
      541 cgccctggcc gacgcggcgc aggcgcaccg cgatctcgag gcccgcaaga cgacgggatc
      601 gagcgtgctc attccctgaa ggtcggcgca tgacgaccct gtacggcacc aagggctcgg
      661 gatcggccgc cgtcgaggcg gcgctcgagg tgatcgggct gccctttcga caggtcgagg
      721 cggcgtcgtg gcagccttcg gccgggctcg acgagctgcg gcgtgtcaat ccgctggcgc
      781 agatcccgac gctcgtcctc gacgacggca gtgtgctcaa cgaaagcgcc gcgatcctga
      841 tcgacctcgg cctcgcgcat ccgcaaagcg gactcctggc gaccgagccg gcgcctcggg
      901 cgcagcagat ccgcggcctc gtctacatcg ccgccaactg ttacgcaggc atcggcatcc
      961 tcgactaccc cgaccgctgg tatccggatc ccgatgacgc cgtcaaggcg aagatgaagg
     1021 agcgcggccg ggcccggctg cacgagctgt gggacctgtt cgccgaccag ttcccggcgt
     1081 cgccgtggct gggcggcccg cgcctgggcg ccctcgacat cctggccgcg acagtctcga
     1141 gatggagtgg cacgcgccag catctgcagg catcgcggcc tgccttctcg gagttgctcg
     1201 cacggatcga cgccgagccg cgcatcgccg cggtctgggc gcggcactgg ccgaagccgt
     1261 agcgcctcgc ctcagcgcga acggcgcacc cgcagcagct cgtcgagcac gagcaggcag
     1321 gcgccgatgg tgatggcgct gtcggcgacg ttgaacgacg ggaagtaggc gccgcgccag
     1381 tgcacctgga tgaagtcgac gacccagccg tgccagaggc ggtcgatgac gttgccgatc
     1441 gcgccgccga ggatcagggc gagcgcccag cggaacagcc gctggccgcc gtggcgggcg
     1501 agcaggaaga cgatgaagac cgcggcgacc gcgcccagac cgatgaagaa ccagcgctgc
     1561 catcccgacg cgccggcgag gaacgagaac gccgcaccgg tgttgtgggc acggacgatg
     1621 tcgaggaagg cgttgacgct gtgcgactcg ttggcgctga acgttcgctg gatgatcgtc
     1681 ttcgtcagct ggtcgaccag gatgacgacg agcgataccg cgagccacgg ccagatcgag
     1741 ggacgtcggg cggccatggt caggccaccg tccgcgtctc gccggcgccg aacaggttgc
     1801 tcgtgcagcg gccgcagatc gtcggatgtg ccggatcgat gccgacgtcg gtgcggtagt
     1861 gccagcagcg ctcgcacttg gccgcgtcgc tgacggcggc atgcacgaag aactcgcccg
     1921 gatcggcggt gtcgccggcc gattcgaggc gcgccgccga cgtgatgagc acgaagcgca
     1981 ggtcgtcgcc gagcgtcgcc agctcggcca ggcgcgtcgg gccggccttg atcgtcactt
     2041 cggcctgcag cgacgagccg agcttgccgt cggcgcgcag cgcctcgatc tgccggttcg
     2101 actcggcgcg gacttcgccg atgcgcgtcc atttcgcgag cagcgcatcg tcccgccacg
     2161 cgcgggtgtc gctgtaggtc tcggcgaaga tcgagaccga atcgcgcggg ccgatcgtct
     2221 tccaggcctc ctcggcggtg aagctgagga agggtgccat ccagcgcagc atcgcctgcg
     2281 cgatctgcgc cagcgccgtc tgcgccgagc gacgcgcacg cgacgcgggc gccgtcgtgt
     2341 agaggcgatc cttcagcacg tcgagataga aggcgccgag gtcttccgag cagaacacct
     2401 gcagcttggc gacgaccgga tggaattcgt agacgccgta gtggccgcca cgaaaaaggc
     2461 cgctcgcggt gtcgaagtcg ccgacgatct cggcctgcag agccgccgcg cgggccaggg
     2521 cccagcgatc gacctcgagc atgtcggcat gcggcacggc gtctttggcg gcatcgaagt
     2581 cgctggtgtt ggcgagcagg aagcgaagcg tgttgcggat ccgccggtag gcgtcgacga
     2641 cgcgcgccag gatgttgggc gtgatcgcca ggtcgcccga gtagtcggtc gacgcgcacc
     2701 agaggcggat gatctcggcg ccgagcttct cggtcgtctc gtgcaggccg acgaagttgc
     2761 ccaggctctt gctcatcttg cggccttcgc tgtcgaccgt gaagccatgc gtcagcaggc
     2821 cgcgatacgg cgcgtggccc tcgagggcgc aggcgatgag cagcgacgag tggaaccagc
     2881 cgcggtgctg gtcatggccc tcgagataga gatcggcctc gggtcgggcg ccatcgtcgt
     2941 cggcgatggt cgtccccgga tggctgccgc gcaggacgtg gaagaaggtc gagcccgagt
     3001 cgaaccagac gtcgaggatg tcgttgctct tggcgtagtg ctcggccgag tgcgggcccg
     3061 tcgcgccgat cacgtcgtcg gcgcccaggc gcgaccaggc ctcgacaccg ccggcttcga
     3121 cgatcgccgc ggcccggtcg acgagggcca gcgtgtcggg gtgcaattcg cccgtcacct
     3181 tgtgcaggaa gaagggcagc ggcacgcccc agttgcgctg ccggctgatg caccagtcgg
     3241 ggcggtgcgc gatcatgtcg tgcagccggg cgcgtccgtt ctcggggtag aacgccgtgt
     3301 cgccgatcgc tgccagcgcg gtttcgcgca gcgcatcggg cgcggggtcg acggcgaaga
     3361 cgcccgccgt cgacgcgtcg ggccggtcca tccgaatgaa ccactgcgcc gcggcgcgat
     3421 agatcaccgg c
//
LOCUS       DASRWS010000277         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1264355, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000277 DASRWS010000000
VERSION     DASRWS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264355"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1163
                     /locus_tag="VFF72_05050"
     CDS             63..1163
                     /locus_tag="VFF72_05050"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZV92561.1"
                     /translation="MEAPRLERRLVAVLAADIVGYSRHMEHDETGALATLSRLRVVAD
                     ELIASHNGRITGTAGDSILAEFSSVVDALDCAVKIQSGVATAGADQAPDKRMQFRIGV
                     NVGDVMVKDGDIFGDGVNIAARLETLARPGGICVSRGVRDHVRKMGRYAFEDLGEQSV
                     KNIAQPIRAFRLHGAEEGVPDAAVPDPPVGLAATPAPPITTPAPFELPFWDAIKESRD
                     PAEFAAYLEKYPSGVFASLAEARRESLLAEQAEPAVPAAPPSDLATMELAFWESIKES
                     TDPVEFAAYLEQYPEGSFATLAEARRQSLLEIEAAPPPENTEADTTELTFWDSVKDSD
                     NPAMYSAYLEKYPEGAFATLAQARIEELATTA"
     gene            1232..1645
                     /locus_tag="VFF72_05055"
     CDS             1232..1645
                     /locus_tag="VFF72_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HZV92562.1"
                     /translation="MHAEAMMSVHPKVHGEVDPVLTRCIEACIDCAQVCRACADACLS
                     EEMVAMLPRCIRLDLDCADLCDATATILARRTSSNDEVLKHLVDICAEACRICADECA
                     RHGAQHAHCKACAETCRACEQACRDASPAILLPRQ"
     gene            complement(1664..2380)
                     /locus_tag="VFF72_05060"
     CDS             complement(1664..2380)
                     /locus_tag="VFF72_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92563.1"
                     /translation="MKNVLLAAFLLLAASGGTALATDELASADLASGGKLNYVLTTRS
                     PATIHYGVILMPGGNGRMGPHMEGAKIAFSGGGNFLIRSREQFAGQQFVTASTDATTT
                     PDRIMAIVHDLERRYGKITIYVIGTSRSTESTMSLAKSLDGQVAGFVHSSSMNAISSF
                     DPRGLKSRNLIVLHKMDACRVTSPSAGISSHNKFGTELIEMDGGKSTGDDCEAYAHHG
                     YYGIERETVDKIKAWILAGK"
     gene            complement(2380..>2586)
                     /locus_tag="VFF72_05065"
     CDS             complement(2380..>2586)
                     /locus_tag="VFF72_05065"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZV92564.1"
                     /translation="SDQLYDQAIALVARERKCSVSFVQRYLQIGYNRAARIVERMERE
                     GVVSSANHVGKREVMARDIDNRER"
BASE COUNT          423 a          904 c          849 g          410 t
ORIGIN      
        1 cttgtggcag agcgggcaaa gcagccggct cgagccccag caccgagcag caggagatcg
       61 tcgtggaagc gccccgtctc gaacgccggc ttgtcgctgt ccttgcggcc gacatcgtgg
      121 gctacagccg ccacatggag cacgacgaga caggcgccct ggccaccctg tcgcgcctgc
      181 gcgtcgttgc ggacgagctg atcgccagcc acaacggccg catcaccggc accgccggcg
      241 acagcatcct cgcggaattc tccagcgtgg tcgacgcact cgactgcgcg gtgaagatcc
      301 agtcgggcgt tgccacggcg ggcgccgacc aggcgccgga caagcgcatg cagttccgca
      361 tcggcgtcaa cgtcggcgat gtcatggtga aggacggcga catcttcggc gacggcgtca
      421 acatcgccgc ccgcctcgag acgctggccc ggcccggcgg catctgcgtg tcgcgcgggg
      481 ttcgcgacca tgtccgcaag atgggccgct atgccttcga ggatctgggc gagcagagcg
      541 tgaagaacat cgcccagccg attcgcgcct tccgcctgca cggcgccgag gaaggcgtgc
      601 ccgatgcggc cgtgcccgat ccgccggtcg gcctcgccgc cacgccggcg ccgcccataa
      661 ccacgccggc gccgttcgag ctgccgttct gggacgccat caaggagagc agggatccgg
      721 ccgagttcgc ggcctacctc gagaaatacc cgagcggcgt cttcgcctcg ttggccgagg
      781 cgcgccgcga gtccctgctg gccgaacagg ccgagcccgc ggttcccgcg gccccgccgt
      841 cggatctcgc gaccatggag ctggccttct gggagtcgat caaggagagc accgatccgg
      901 tcgagttcgc ggcctatctc gaacagtatc cggagggatc gttcgccacc ctcgccgagg
      961 cccgccgcca gtcgttgctt gagatcgagg cggcgccccc gcccgagaac accgaggccg
     1021 acacgacgga gctcaccttc tgggactcgg tcaaggacag cgacaatccg gcgatgtact
     1081 cggcctatct cgagaaatat cccgagggcg ccttcgccac cctggcccag gcgcgcatcg
     1141 aggaactggc gaccacggcc tgaaagcggc gaaactcgac aggtgcgccg ggcgttgaag
     1201 agacaacccc cttcaccgca aggagacgcc catgcacgcc gaagccatga tgtcggtcca
     1261 tcccaaggtc catggcgagg tcgacccggt cctgacccgg tgcatcgagg cctgcatcga
     1321 ctgcgcccag gtctgccgcg cgtgcgccga tgcctgcctc tccgaggaga tggtggcgat
     1381 gctgccgcgc tgcatccggc tcgacctcga ctgtgccgac ctgtgcgacg ccacggctac
     1441 gatcctggcg cgtcgcacca gcagcaacga cgaggtactg aagcacctgg tcgacatctg
     1501 cgccgaggcc tgccgcatct gcgccgacga atgcgccagg cacggcgccc agcatgccca
     1561 ttgcaaggcg tgcgcggaga cctgccgcgc ctgcgagcag gcctgccgcg acgcctcgcc
     1621 cgcgatcctg ctgccgcgcc agtgacggat acggattggg cggctacttg ccggccagga
     1681 tccacgcctt gatcttgtcg acagtctcgc gctcgatgcc gtagtagccg tgatgggcat
     1741 aggcctcgca gtcgtcgccg gtcgacttgc cgccgtccat ctcgatcagc tcggtgccga
     1801 acttgttgtg cgacgagatt ccggccgatg ggctggtcac cctgcaggcg tccatcttgt
     1861 gcaggacgat caggttgcgg ctcttcaggc cgcgcggatc gaagctcgag atggcgttca
     1921 tcgacgagct atgaacaaag cctgcgacct gcccgtcgag cgacttggcc aatgacatcg
     1981 tgctctcggt gctgcgcgag gtgccgatca cgtagatcgt gatcttgccg tagcggcgct
     2041 cgagatcatg cacgatcgcc atgatgcggt cgggcgtcgt cgtggcgtcg gtcgaggcgg
     2101 tcacgaactg ctggccggcg aactgctcgc gcgagcggat caggaagttg ccgccgcccg
     2161 aaaaggcaat cttcgccccc tccatgtggg gacccattcg cccgttgccg cccggcatca
     2221 ggatgacgcc gtagtggatc gtggccgggc tacgcgtcgt cagcacatag ttgagcttgc
     2281 cgcccgacgc gagatcggcg ctcgccagct cgtccgtcgc cagcgccgtg ccgccgctgg
     2341 ccgccagcag caggaaagcg gccaacagaa cgttcttcat caacgctccc ggttgtcgat
     2401 gtcgcgcgcc atgacctcgc gcttgccgac gtggttggcc gagctcacca cgccctcgcg
     2461 ctccatacgc tcgacgatgc gggcggcgcg gttgtagccg atctgcaggt agcgctggac
     2521 gaaactcacg ctgcacttgc gctcgcgggc gaccagcgcg atcgcctggt catagagctg
     2581 gtcgct
//
LOCUS       DASRWS010000278         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2989390, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000278 DASRWS010000000
VERSION     DASRWS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989390"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFF72_05070"
     CDS             complement(<1..1007)
                     /locus_tag="VFF72_05070"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZV92565.1"
                     /translation="MRPQSLTPLFAQVTSLPGIGPRLGKLVERLTGSLVLDLLWHLPV
                     GVIDRRNAPTVAKAQPGTVATLTVTVDEHIVSHNRRAPYRVWCSDKSGRLCLTYFNGR
                     EDYLKKLLPIGERRVISGKVELYQGDVQMTHPDHVVPVEELELILRVEPVYGLTTGLT
                     QRPLQKAITAAVERAPELPEWQDAAYLKKQKWPGWKVALTTAHAPADETDLSPMTPAR
                     ARLAFDELLASQLAIALVRHHNRSLPGRTTKGDERLQKRALAALPFKLTPSQTFATAE
                     IAGDMAKPERMVRLLQGDVGSGKTLVAFLAMLTAVEAGAQAALMAPTELLARQHYATI
                     AP"
     gene            1093..1362
                     /locus_tag="VFF72_05075"
     CDS             1093..1362
                     /locus_tag="VFF72_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HZV92566.1"
                     /translation="MGDDTELELRRKRLLYRSIYRGNKENDILLGQFARAHIAEFSAA
                     ELDQYERLLATGDNDIFDWVTGKVAVPPDMDTMVVRKLLAFRVRF"
     gene            1362..>2582
                     /locus_tag="VFF72_05080"
     CDS             1362..>2582
                     /locus_tag="VFF72_05080"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92567.1"
                     /translation="MAGLADRLASLHRKAGRWTLAGLPEGADAMVLAELAKTTGGQDI
                     LHVARDGQRLERLQDGLRFFAPEREVLVFPAWDCLPYDRLSPHPDIVAERLETMARLA
                     TPKKPGAPARVILASVGAALQKVPPRSLYAEAAKVLRKGENVDVNALTKYLGENGYGR
                     ADTVMEPGEYAVRGGLVDLFPPGTNEPLRLDLFGDVLETIRSFDPMSQLTTGERDEVV
                     LLPVSEVLLTPDSIERFRVGYRELFGNVAGDDLLYEAVSAGQRYAGMEHWLPLFHERL
                     ETLIDYCPEAIVTADHQIKEAFDARWELIDDYHDARVKTGAKGGMSGGADYKPLPTDR
                     LYLKPSQWDNLLEGHSVGQFTSFDAPPEAPNAVDLGGRRHEGFAQARTAQGVNLFDKV
                     SDHVKAANAAGRRVV"
BASE COUNT          404 a          884 c          890 g          404 t
ORIGIN      
        1 ggcgcgatgg tggcgtagtg ctggcgggcc agcagctcgg tcggcgccat cagggccgcc
       61 tgcgcgcctg cctcgaccgc ggtcagcatg gcgaggaagg cgaccagggt cttgccgctg
      121 ccgacatcgc cctgcagcag ccgcaccatg cgctcgggct tggccatgtc gccggcgatc
      181 tccgcggttg cgaaggtctg gctcggcgtc agcttgaacg gcagcgccgc cagcgcgcgc
      241 ttctgcagac gctcgtcgcc cttggtggtg cggccgggca gggagcgatt gtggtggcgc
      301 accagggcga tcgcgagctg gctcgccaac agctcgtcga aggcgagccg cgcccgggcc
      361 ggagtcatcg gcgagagatc tgtttcatcg gccggggcgt gcgcggtcgt gagcgcgacc
      421 ttccagcccg gccatttctg cttcttgaga taagctgcgt cctgccattc gggcagctcg
      481 ggcgcgcgct cgaccgcggc ggtgatcgcc ttctgcaagg ggcgctgcgt cagcccggtg
      541 gtcaggccgt agaccggctc gacgcgcagg atcaattcga gctcctcgac cggcacgacg
      601 tggtcgggat gcgtcatctg cacgtcgccc tggtagagct cgaccttgcc gctgatgacg
      661 cgccgctcgc cgatcggcag cagcttcttg agatagtcct cgcggccgtt gaagtaggtg
      721 aggcagagcc ggcccgactt gtcgctgcac cacacgcgat agggcgcccg ccgattgtgc
      781 gacacgatgt gctcgtcgac cgtgacagtg agggtcgcaa ccgtacccgg ctgtgccttg
      841 gcgaccgtcg gcgcgttgcg ccgatcgatc acgccaaccg gcagatgcca cagaagatcg
      901 agcaccagcg aacccgtcag gcgctcgacg agcttgccca gccgcggccc gattccgggc
      961 agcgacgtca cctgggcgaa caggggggtg aggctctgcg gacgcatggc gcgtatgggt
     1021 tctctgggac gtgacttcgc accccaccgc gcctatatgc tacgcccctt tttgacgaag
     1081 cctgtgggaa aaatgggcga cgacactgaa ctggagctgc ggcgcaagcg tttgctctat
     1141 cgcagcattt atcgcgggaa caaggagaac gacatcctcc ttggccagtt cgcgcgcgcc
     1201 catatcgcgg aattctccgc cgcggagctc gaccagtatg aacgcctgct cgccaccggc
     1261 gacaacgaca tcttcgactg ggtcaccggc aaggtcgcgg tgccgccgga catggacacg
     1321 atggtcgtgc gcaagcttct cgcattccgc gtgaggttct agtggcaggc ctcgccgacc
     1381 gcctcgccag cctgcaccgc aaggccggac gctggaccct cgccgggttg ccggagggcg
     1441 ccgacgcgat ggtgctggcc gagctcgcca agacgaccgg cgggcaggac atcctgcacg
     1501 tcgcgcgcga cggccagcgg ctggagcggc tgcaggatgg cctgcgcttc ttcgcgcccg
     1561 agcgcgaggt gctggtgttc ccggcgtggg attgcctgcc gtacgaccgc ctgtcgccgc
     1621 atcccgacat cgtcgccgaa cggctggaga cgatggcgcg gctggcgact ccgaagaagc
     1681 cgggcgcccc ggcgcgtgtc atcctcgcct cggtcggcgc agcgctgcag aaggtgccgc
     1741 cgcgtagcct ctacgccgag gcggccaagg tcctgcgcaa gggcgagaac gtcgacgtca
     1801 acgcgctcac caagtatctc ggcgagaacg gctatggccg cgccgacacg gtgatggagc
     1861 cgggcgagta cgccgtgcgc ggcggcctgg tcgatctctt cccaccgggc accaacgagc
     1921 ccttgcgcct cgacctgttc ggcgacgtgc tggagaccat ccgctcgttc gatccgatga
     1981 gccagctcac caccggcgag cgcgacgagg tcgtgctgct gccggtcagc gaggtgctgc
     2041 tgacgcccga cagcatcgag cgctttcgcg tcggctatcg cgagctgttc ggcaacgtcg
     2101 cgggcgacga cctgctctac gaggcggtgt cggccggcca gcgctacgcc ggcatggagc
     2161 actggctgcc attgttccac gagcggctcg agacgctgat cgactactgc cccgaggcga
     2221 tcgtcaccgc cgatcaccag atcaaggaag ccttcgacgc gcgctgggag ctgatcgacg
     2281 actatcacga cgcccgcgtg aagaccggcg ccaagggcgg catgtcgggc ggcgccgact
     2341 acaagccgtt gccgaccgac cggctctacc tcaagccgtc gcagtgggac aacctgctcg
     2401 agggccatag cgtcggccag ttcaccagct tcgacgcccc gcccgaggcg cccaacgcgg
     2461 tcgatctcgg cggccgtcgc cacgaaggat tcgcccaggc gcgcaccgcg cagggcgtga
     2521 acctgttcga caaggtgtcg gaccacgtga aggccgccaa cgccgccggc cgccgcgtcg
     2581 tg
//
LOCUS       DASRWS010000279         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_112567, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000279 DASRWS010000000
VERSION     DASRWS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112567"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFF72_05085"
     CDS             <1..194
                     /locus_tag="VFF72_05085"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346770.1"
                     /note="catalyzes the formation of pyruvate and
                     beta-alanine from L-alanine and 3-oxopropanoate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZV92568.1"
                     /translation="IELEAVPGSPGKRGFDVLRACFERGVLIRTTGDIIALSPPLIVE
                     ESHIDQAISTIADVLKTVA"
     gene            313..1425
                     /gene="potA"
                     /locus_tag="VFF72_05090"
     CDS             313..1425
                     /gene="potA"
                     /locus_tag="VFF72_05090"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HZV92569.1"
                     /translation="MSAPAFLQISHIVKDFDGHRAVDDVCIDIAKGEVFALLGSSGCG
                     KTTLLRMLAGFETPSSGSITLDGRDLAGLPAYERPMNMMFQSYALFPHLSVSDNIAFG
                     LRRDRLARDEIAARVDAMLRLVQLTGYAKRKPHQLSGGQQQRVALARSLAKKPQLLLL
                     DEPLGALDRKLREETQIELANIIHEVGVTCVMVTHDQEEAMTMASRIAVMSAGRFLQV
                     GAPAEIYETPASRFVADFIGNVNLMDGTVVEADADHVVVDCGDLRHWIDASADIAPGA
                     KVSVALRPEKIRIGRGAAPPPTAQEPAAPFNRVRGKVRDLAYFGSFTVYRLQLEGGRV
                     LKVSQSNTERQTEGALSWDDEAWAAWSPSSPIVLTQ"
     gene            1422..2354
                     /locus_tag="VFF72_05095"
     CDS             1422..2354
                     /locus_tag="VFF72_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV92570.1"
                     /translation="MIRPGRSGWPRGRAAVIGVPYVWLLVFFLLPFLVVAKISFSEME
                     TTSVASIVSWHDGGIHFNAHLDTYLQLVRDDLYLRTYAASLKYAAVTTLLCLVIGYPF
                     AYFMARARRTLQPALLMLVMLPFWTSFLLRVYAWKALLTGHGLAANAIEATGFDKVLA
                     AAGMIPGPGQLMNTPFSLVLGMTYAYLPFMILPLYSNLAKMDLRLLEAASDLGASAWT
                     AFWTVTVPLSRAGIIAGTMLVFIPCVGEYVIPELLGGPATQMIGRTLWDEFFSNNDWP
                     MACTVAVAMILFVIVPIAIFSRYQSEATDRRAPA"
     gene            2351..>2800
                     /locus_tag="VFF72_05100"
     CDS             2351..>2800
                     /locus_tag="VFF72_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HZV92571.1"
                     /translation="MKTATFNRAFALGWLGLGYAFLYIPIVALVVYSFNESPIPNEWR
                     GFTVKWYAALASDDELLAGLVLSLKVALSTACAAIVLGTLAAFAMTRYRRFFGSSLFA
                     GMVAAPLVMPEVIIGLSLLLMLVSLQRATEAIFGVGFPERGMLTIWMG"
BASE COUNT          423 a         1001 c          876 g          500 t
ORIGIN      
        1 cgatcgagct cgaagcggtt ccgggcagcc ccggcaagcg aggcttcgat gtccttcgcg
       61 cctgtttcga acgcggtgtg ctgatccgca ccaccggcga catcatcgcc ctctcgccgc
      121 cgctgatcgt cgaggaatcg cacatcgacc aggcgatcag cacgatcgcc gatgtgctga
      181 agacggtcgc ctgacgcgac cggtttccgg cggcagggca gtgcctcgcc gccgaccggc
      241 gcgtggcacg ccgtcgaagg tgttggacaa ttcgcggttt gcccgccgcc ccgggcgacg
      301 tcacgctgcc cgatgagcgc tccggccttc ctgcagatca gccacatcgt caaggacttc
      361 gacggccacc gcgccgtcga cgacgtctgc atcgacatcg ccaagggcga ggtcttcgcc
      421 ttgctcggct cgtcgggctg cggcaagacg acgctgctgc gaatgctcgc cggcttcgag
      481 accccgagct cgggatcgat cacgctcgac ggccgcgacc tcgccggcct gcccgcctac
      541 gagcggccga tgaacatgat gttccagagc tacgcgctct tcccgcatct ttcggtcagc
      601 gacaacatcg ccttcggact gaggcgcgac cggctggcca gggacgagat cgccgcccgc
      661 gtcgacgcga tgctgaggct cgtacaactc acgggctacg ccaagcgcaa gccccaccaa
      721 ctttccgggg ggcagcaaca acgtgtcgcc ctggcgcgaa gcctggcgaa gaaaccccag
      781 ctcctgctcc tcgacgagcc gctcggcgcg ctcgaccgga agctccgcga ggagacgcag
      841 atcgagcttg ccaacatcat ccacgaggtc ggcgtcacct gcgtcatggt gacgcacgac
      901 caggaagaag cgatgacgat ggcctcgcga atcgccgtga tgagcgccgg gcgattcctc
      961 caggtcgggg cgccggccga aatctacgag acgccggcga gccgattcgt ggccgacttc
     1021 atcggcaacg tcaacctcat ggacggcacc gtcgtcgagg ccgacgccga ccacgtcgtc
     1081 gtcgactgcg gagacttgcg acactggatc gacgccagtg cggacatcgc gccaggagcc
     1141 aaggtgtccg ttgcgctgcg tcccgagaag atccgcatcg gccgcggcgc ggcgccgccg
     1201 ccgacggcgc aggaacctgc cgcgcctttc aaccgggtgc gcggcaaggt ccgcgacctc
     1261 gcctacttcg gcagcttcac cgtctaccgg ttgcagctcg agggcggacg cgtcctgaag
     1321 gtcagccaga gcaacaccga acgacagacc gaaggcgcgc tctcctggga cgacgaggcg
     1381 tgggcggctt ggtcgccgtc gtcgccgatc gtcctgaccc aatgatccgg cccgggcgct
     1441 ccggctggcc gcgcggccgt gcggccgtga tcggcgttcc gtacgtctgg ctgctggttt
     1501 tcttcctcct gccttttctc gtcgtcgcca agatcagctt ctccgagatg gagacgacga
     1561 gcgtcgccag catcgtttcc tggcacgacg gcggcatcca cttcaacgcc catctcgaca
     1621 cctatctgca gctcgtccgc gacgatctct acctgcgcac ctacgcggcg agcctcaagt
     1681 acgccgcggt gacgacgctg ctgtgcctgg tcatcggcta cccgttcgcc tacttcatgg
     1741 cgcgagcgag gcgcaccctg cagccggcgc tgctgatgct cgtcatgctg ccgttctgga
     1801 cctccttcct gcttcgcgtc tatgcctgga aggcgctgct caccgggcac ggcctggccg
     1861 cgaacgcgat cgaggcgacc gggttcgaca aggtgctcgc cgcggccgga atgatccccg
     1921 gaccgggaca gctcatgaac acgccgttct cgctcgtcct cggcatgacc tacgcctatc
     1981 tgccgttcat gatcctgccg ctctattcga acctggcgaa gatggacctg cgcctgctcg
     2041 aggccgcctc cgacctcggc gcttcggcgt ggacggcgtt ctggacggtg accgttccgc
     2101 tgtcgagggc cggcatcatc gccgggacga tgctcgtctt cattccctgc gtcggcgagt
     2161 acgtgattcc cgagctcctc ggcgggccgg cgacgcagat gatcggccgc acgctctggg
     2221 acgagttctt cagcaacaac gactggccga tggcgtgcac cgtcgccgtc gcgatgatcc
     2281 ttttcgtgat cgtgccgatc gccatcttca gccgctacca gtcggaagcc acggatcgcc
     2341 gggcgcccgc atgaagacgg ccaccttcaa tcgcgccttc gcgctcggct ggctcggcct
     2401 gggctatgcc ttcctctaca tccccatcgt cgccctcgtc gtctattcgt tcaacgagtc
     2461 gccgatcccg aacgagtggc gcggcttcac cgtcaagtgg tacgccgccc tcgcctccga
     2521 cgacgagctt ctcgccggcc tggtgctgtc gctgaaggtg gcgctctcga cggcgtgcgc
     2581 cgcgatcgtc ctcggcacgc tcgccgcctt cgcgatgacg cgctaccggc gtttcttcgg
     2641 cagctcgctt ttcgccggca tggtcgcggc gccgcttgtc atgcccgaag tgatcatcgg
     2701 cctgtcgctg cttctcatgc tcgtgtcgct gcagcgcgcc accgaagcga tcttcggcgt
     2761 cggctttccg gagcgcggca tgctgacgat ctggatgggc
//
LOCUS       DASRWS010000280         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_113512, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000280 DASRWS010000000
VERSION     DASRWS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113512"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFF72_05105"
     CDS             <1..448
                     /locus_tag="VFF72_05105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZV92572.1"
                     /translation="AMFATGVTIVTANDANGQPVGLTANSFNSVSLRPPLVLWSLSRQ
                     AGSMPAFEQGSHYAINILAAEQHALAERFASHGLDRFDGVAFRAGAGGVPLLDGSAAV
                     FECFNRSRYEEGDHVIFVGEVERCSRRAGAQPLIFHGGRYFTELPL"
     gene            466..945
                     /locus_tag="VFF72_05110"
     CDS             466..945
                     /locus_tag="VFF72_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZV92573.1"
                     /translation="MAAALVLAASSGTVPAAPAERMLMNPFHDPFVQALAGRPCPPPL
                     GPAYTDAEIRLEEHSRVERGTSCWLAGKCAEPNAYRYDAANAKAAVAALRSDASLATG
                     AIWVTAQRRFIYLEGCVADAAQAARAEAAVKALADVDRVIPALSLPGERAPYARAPR"
     gene            complement(924..>2625)
                     /locus_tag="VFF72_05115"
     CDS             complement(924..>2625)
                     /locus_tag="VFF72_05115"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZV92574.1"
                     /translation="QSGDRVVAFMPNVPQTAIAFLACASLGAVWSVCSADMGPLAVLD
                     RFRQIEPVLIVACDGYTYGGAEHDRMAVLAELLAGLPTLRDVVLWRNLDAAADPGALA
                     SPTRRAHDFERLVASKQTLAPRSLAFDHPLWIVYSSGTTGLPKAIVHGHGGVMLEALK
                     SGALHNNVGSSVDTGDRYHWYSSTGWIMWNSQMGALLGGTTVCMYDGSPAGPLSARAG
                     ADRVVDWSTVWRFAAATGVTFFGAGAAFYASCLKEGVEPQQAGDLSAIRAIGSTGSPL
                     AVDCYEWIWDKLPKVDGRHVWLNPISGGTDFAGAFVAGMATLPVVAGEMQCRCLGAAV
                     EAWGEPDAEGHGESLVDAVGELVCTRPMPSMPLYLWGDEPPGPHGRRYHASYFDMYMS
                     RDGRHPVWRHGDWIRITPRGGAIIYGRSDATINRHGIRMGTAELYRAVEALPEVLDSL
                     VVDLEFLGSESWMPLFVVLRDDMQLDDALAARINAVIRQALSARHVPNAIFQVKAVPR
                     TLSGKKMELPVKKLLMGAPADDVFKTGAMANGESVAWFIELAEKRAAGRPASQRGALA
                     "
BASE COUNT          395 a          930 c          911 g          389 t
ORIGIN      
        1 cgcgatgttc gcgaccgggg tgacgatcgt caccgccaac gacgccaacg gccagcctgt
       61 cggtctcacg gccaattcgt tcaactcggt gtcgctcagg ccgccgctcg tcctctggag
      121 cctgtcgcgc caggccggtt cgatgccggc attcgagcaa ggctcgcact acgcgatcaa
      181 tatcctcgcg gccgagcagc acgccctggc cgagcgattc gcgagccacg gtctcgaccg
      241 gttcgacggc gtcgccttcc gggccggtgc cggcggtgtc ccgctgctcg acgggtcggc
      301 ggccgtcttc gagtgcttca atcgcagccg ctacgaggaa ggcgaccacg tcatcttcgt
      361 cggcgaagtc gagcgctgct ctcgccgtgc cggcgcgcag ccgctcatct tccacggggg
      421 acgctacttc acggagctgc cgctttgacg cgaatcgggc gacgtgtggc ggctgccctc
      481 gtcctcgcgg cctcgtcagg caccgtgccc gccgcgccgg ccgagcgcat gctgatgaat
      541 ccgttccacg acccgttcgt gcaggcgctc gccgggcggc cgtgcccccc gccgctcggg
      601 ccggcctata ccgacgcgga gatccgtctc gaggaacatt cgcgggtcga gcgcggcacc
      661 tcgtgctggc tcgcgggcaa gtgcgccgag ccgaacgcct atcgctacga cgcggccaac
      721 gcgaaagccg ctgtcgccgc attgcgcagc gacgcatcgc tcgcgaccgg cgcgatctgg
      781 gtcaccgcgc agcgccgctt catctacctc gagggttgcg tcgccgacgc ggcccaggcc
      841 gcacgcgccg aggccgccgt caaggcgctt gccgacgtcg accgcgtcat tccggcgctg
      901 tctctgcccg gtgaacgggc gccctacgcc agggcgccgc gctgagatgc cgggcgaccg
      961 gccgcgcgct tctcggccag ctcgataaac cacgccaccg attcgccgtt ggccatggcg
     1021 ccggtcttga agacatcgtc ggccggcgcg cccatcagga gcttcttcac gggcagttcc
     1081 atcttctttc ccgacagcgt cctcggcacc gccttgacct gaaagatcgc gttcgggacg
     1141 tggcgcgccg acagcgcctg gcgaatgacc gcgttgatgc gcgccgcgag cgcgtcgtcg
     1201 agctgcatgt cgtcgcgcag gacgacgaag agcggcatcc agctctcgct gccgaggaac
     1261 tcgaggtcga cgacgaggct gtcgagcact tcagggagcg cctcgacggc gcgatacagc
     1321 tcggccgtgc ccatgcggat gccgtgccgg ttgatcgttg catcgctgcg gccgtagatg
     1381 atcgcgccgc cgcgcggcgt gatgcggatc cagtcgccgt gccgccagac gggatgtcgg
     1441 ccgtcgcggc tcatgtacat gtcaaagtag ctcgcgtggt agcggcggcc gtgcggcccc
     1501 ggcggctcgt cgccccagag gtagagcggc atcgacggca tcggccgggt gcagacgagc
     1561 tcgccgacgg cgtcgacgag ggattcgcca tggccctcgg cgtcgggctc gccccacgct
     1621 tccacggcgg cgccgaggca gcggcattgc atctcgccgg cgacgaccgg cagcgtcgcc
     1681 atgccggcga cgaaagcgcc ggcgaagtcg gtgccgcccg agatcggatt gagccagaca
     1741 tggcgtccgt cgaccttcgg cagcttgtcc cagatccatt catagcagtc gacggccagc
     1801 ggcgagccgg tcgagccgat ggcgcggatg gccgacaggt cgcccgcctg ttgcggctcg
     1861 acgccctcct tcaggcaact ggcgtagaag gccgcgccgg caccgaagaa agtgacgccg
     1921 gtcgccgccg cgaagcgcca gaccgtcgac cagtcgacga cgcggtctgc accggcccgc
     1981 gccgagagcg ggccggccgg gctgccgtcg tacatgcaca ccgtggtgcc gccgagcaag
     2041 gcacccatct gcgagttcca catgatccag ccggtcgagc tgtaccagtg gtagcgatcg
     2101 ccggtgtcga cactgctgcc gacgttgttg tgcagcgcgc ccgacttgag cgcctcgagc
     2161 atgacgccgc cgtggccgtg gacgatcgcc ttcggcaagc cggtcgttcc gctcgagtag
     2221 acgatccaca gcggatgatc gaaagccagc gaacgcggcg cgagcgtctg cttcgaagcg
     2281 acgagacgct cgaagtcgtg ggcccgtcgc gtcggcgagg cgagggcccc gggatctgcc
     2341 gcggcgtcga ggttgcgcca gaggacgacg tcgcgcagcg tcggcaggcc ggcgagcaac
     2401 tcggcgagca cggccatccg gtcgtgttcg gcgccgccgt aggtgtagcc gtcgcaggcg
     2461 acgatcagca cgggctcgat ctggcggaac cggtcgagca cggccagcgg ccccatgtcg
     2521 gccgagcaca ccgaccagac ggcgccgaga ctggcgcagg cgagaaaggc aatcgccgtc
     2581 tgcgggacgt tcggcatgaa ggcgacaacc cgatcgccgg attga
//
LOCUS       DASRWS010000281        11107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_113687, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000281 DASRWS010000000
VERSION     DASRWS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113687"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFF72_05120"
     CDS             <1..328
                     /locus_tag="VFF72_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92575.1"
                     /translation="VVKKRSEELFAEFEKRLRAELVSVDQGQLPTADVDAGQPPRSVE
                     NAALAPVEAKRARGGLTLSGPRAGIGLALGVVVALAASVFGGSPWWWLALPALAVAGG
                     LRRRNA"
     gene            328..1191
                     /locus_tag="VFF72_05125"
     CDS             328..1191
                     /locus_tag="VFF72_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZV92576.1"
                     /translation="MRPHPFEFHRAAGLAEALALKASHGDEAAFYAGGTELLVALKAR
                     VLQYTHLIDIKRIAELRGIRPGKEGGLVIGALSTHHGISNDPLVRRLLPAYAELSEQV
                     GNIRVRIAGTVGGVLCFAEPHADAPTLLCALDARLRLAGADGERTLPSRDFHLGEFTT
                     ARRDDELLVSIEVPPQPEGSRAAYRCFGKAERPAVGVAAVWREEGPSRLSLWAGAIVG
                     APTRLVQTEKAAGELPTGLNAEQVWQRLQPVVAAEAARLDASDDLHGGADYKKHLVAV
                     LAKRAIEACLS"
     gene            1188..1700
                     /locus_tag="VFF72_05130"
     CDS             1188..1700
                     /locus_tag="VFF72_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZV92577.1"
                     /translation="MSSQAPKPASTRVDFVLNGHAVTVEIEPRELLIDVLRERLRLKG
                     TKRSCDVQVCGACTVLVDGLPVSSCTTLCVDAHEHTLTTIEGLAGEGRLDPVQRAFIA
                     HGALQCGFCTPGMILAVRSLLSQHAAPSDETIRHYLRGNICRCTGYVKIMEAIQDLVR
                     DPSRYQTEQK"
     gene            1697..2935
                     /locus_tag="VFF72_05135"
     CDS             1697..2935
                     /locus_tag="VFF72_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZV92578.1"
                     /translation="MMRDARPDECMRVVIAGAGMGGLTLALALLQKGIDVDVYERSPE
                     LREVGAGLWISANGSRVLAALGLQGAIDAINLPPEDRVVRFWRTGDEHSVYVRDNRES
                     AVGRVPKADHTLVQVLRAELQRVLYEAVCALKPDAVHFDAHVLGAANVASGAQLLIEG
                     QDAVEGAVVVGADGAHSRVRQSQFGPAPARYTGAVAWRGLAPMRLLKPQHRRALASTW
                     VGPRAHVTTYPVRRGTEEFVSFSAQVDSETWHTDSWFEPGQLEDALRDFEGWHQDILD
                     LFINSESLFRWGLFVRDPLEAWSRGRVTLLGDACHSMTPYLGMGVNITMEDACVLARC
                     LEAAPAEFDHALRRYDAARIERANRTKDESLKMHRIFHSPELARADTARSYIQKQWSP
                     QVVRDRYDWLLKYDATTADI"
     gene            2939..3655
                     /locus_tag="VFF72_05140"
     CDS             2939..3655
                     /locus_tag="VFF72_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZV92579.1"
                     /translation="MSTIVSSPKPRPWDGIIPAEELAIYERAGWGAPGGMGCRPAMLV
                     IDVQYRSMGERSLPIHEAIRESATSCGEYGWRALPHIAALLAVFREMRAPVLYPHVAF
                     KGPHNRGQFEAKVPGVMDVPLRGYDFVAEVAPRTEDITIPKFQASAFHATALTSHLVG
                     LGVDTLIVTGCTTSGCVRATVVDACALNFKVVVPQDAVYDRSQTSHAVNLFDMASKYA
                     DVLPTSELIEQLRSGPVQCR"
     gene            3670..4452
                     /locus_tag="VFF72_05145"
     CDS             3670..4452
                     /locus_tag="VFF72_05145"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92580.1"
                     /translation="MSPPGVPLLSIDRVTKTYETRRGEQVLAIGEVTLDLADQEFVSL
                     IGPSGCGKSTLLHIAAGLLKPTRGDVRVRGESRPIHAKEFGMVFQDAVLFPWRTIREN
                     VEMPAEVLGLPKDVYKPRAQELLELVGLAACGAMYPRELSGGMQQRASIARSMIHDPE
                     LLLMDEPFGAVDAITRENLNIELQRIRTLARKSVLFVTHSIAEAVFLSNRVVIMSAKP
                     SRVSAVVNIDLPADRGSELMESVELNDLVRRIHRELGGHAHP"
     gene            4490..5290
                     /locus_tag="VFF72_05150"
     CDS             4490..5290
                     /locus_tag="VFF72_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92581.1"
                     /translation="MQRENVLEHAFTGALVAGFSIFLVIAWQLVTSWHLLPTYLLPTP
                     AETMQAIWKDLTSVSFWTQQFLQTLLVVIVGFVIAAAAGLIVGPLIISHPLVERTLYP
                     FVVAFQTMPKIAMAPLLLVWLGYGHTSKFLITALVAFFPVVVNAIAGLKAGDERQLIL
                     MRSLRASWWTRMTKVRIPNALPYIFAGLDIAVVFAVIGAIVAEFLGSANGLGSLVIVR
                     QTNVDIAGLFSVLFFLAVMGSALHFIIASLGRKFTFWSQSTDAKSARR"
     gene            5358..6467
                     /locus_tag="VFF72_05155"
     CDS             5358..6467
                     /locus_tag="VFF72_05155"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92582.1"
                     /translation="MKMYAVAIGAAIAAFGGTSFGADAAANSSAASQTRAWISGVPAP
                     GAIKPAEERDVVVQGASPAPNIGYLALYVAQKMGYFKDEGLDVKINYSHGDSAPLQAI
                     GAGQAQIMSGTPEALIHGYERGLRGVLFYQTYDKLIFSVAVLKDGKITKPAELVGKTI
                     GVSSIASTCVLIAKVLVSDAGADPAGVKFLPVGTGQQALGALNSGQVDALCLWDAAYS
                     QIETGANKLTLVHWQPASLTHVGDGGYFTSWELIQKQPNMLAHFTRAIAKAMVAIHKN
                     PSGALQIYWEVNPSAKPKGSDAQALQTGLEQLQIVGRSLDLSGVPKPVDEASLNAYVK
                     TFLAQGVIQKSIPVEQIVTNAFVPIAKEAAGEASK"
     gene            6520..7566
                     /locus_tag="VFF72_05160"
     CDS             6520..7566
                     /locus_tag="VFF72_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase"
                     /protein_id="HZV92583.1"
                     /translation="MKPDAETEMLSLFHAELSLCKVCPGETVAVLTANNEKADYAQAC
                     MIAAQQLGATAYQINVPRAQTRAAGVQGRHPLAGNLPAIESAKRADLLIDLMGLLFSK
                     EQAEIQASGTRVLRVVEPLHVLKQMFPTEDLRRRVEFAKQLLESAKELRFTSPAGTDV
                     RYSLSQYPVICEYGYTDQPGRWDHFPSGFSFTQANDGGVDGIVVLRPGDIFAAFKRYV
                     QTAVRLTIRAGIIVEIDGEGMDAALMRSYIEGFGDPRGYAVSHIGWGLNERAQWHQFA
                     TSQHVQSEYVMNALSFYGNVLFSTGPNTELGGSNDTPCHLDLPMRGCSLWLDGVPILE
                     DGRVVHPQMKVPGR"
     gene            7636..8841
                     /locus_tag="VFF72_05165"
     CDS             7636..8841
                     /locus_tag="VFF72_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZV92584.1"
                     /translation="MAESGAPVLIAGAGLGGLAAGLALLRKGIDVEIFEQSASLGEVG
                     AGVQISANGTTVLFALGLEEDVRRDGVPVTDKEIRLWNSGQSWSLFKSDPLPAGGRYE
                     HPMFTLHRKDLHAMLVRAVRELKADAIHLGRRCVRFEATDADVRLAFEQGPPAAGSIL
                     IGADGLHSRIREQLCGPMDAEFTGQLAWRGMVPQDRLTPQMRRLVGTNWVGPSAHVTC
                     YPVQRGEVFNFVGQVDRTDWRVESWVTEGSTEECLADFSGWHPQVRHMIESAPKLFKW
                     ALFLRQPLPRWSVGRVTLLGDACHAMLPYLGQGANMALEDGFVLARCLAAASTDPQVA
                     LLRYENARRERTEKIVRASAEMARTFHNPALAEAASAQRYVETQWSGGMVRTRYDWIY
                     GYNASTSPI"
     gene            8875..9837
                     /locus_tag="VFF72_05170"
     CDS             8875..9837
                     /locus_tag="VFF72_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92585.1"
                     /translation="MKAVFRCAWLICLAMVAPLAVAQAYPEHAVKLVVPFPAGATMDA
                     VVRLIGEKLGSRWGQAVVVENRTGAAGLIGTASVAKAVPDGYTILAVANSFAANPTLR
                     SSMPYDTAKDFVPITLIGSTPLVLVANPHLPASSTAELVALAKKEPGRIPYGAAAGAS
                     PHLAMAWLESVAGIKLLFVPYRGQSQAQTDLLGGQVKLVFGNLPDILPLVKDGQLRAL
                     GIATGTRSPLAPDIPTLAEAGYKGPQWDSWYGFVAPVGTPAAIVDKFAADVRAVLAMP
                     DVKEALRSRGIVAVGDTPAEFDAFLKRTADDYAKIIRQAHIHVD"
     gene            9827..10642
                     /locus_tag="VFF72_05175"
     CDS             9827..10642
                     /locus_tag="VFF72_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV92586.1"
                     /translation="MSIEPRGQSYGAHVRANEIRQHYLRFPGAGQALIVVPGITSPAA
                     TWDFVGERLGRIFDTYVLDVRGRGLSQGGSDLSYDLDTCASDVEQFASALGLRDFALL
                     GHSMGGRIALRLAGRGGTAISRLVLADPPLSGPGRRPYGRSLDFYLEAIRRARAGTLD
                     AEAVRQTYPRWSDAQLRSRAQWLHTCDETAVAASVRGFQEDDIHVDFARLHLPTLLLA
                     AGSGGVVSDEDAAEVLRLMPSLEIRRLASAGHMLPFDDLEGFLAIAGEFLKRN"
     gene            10671..>11107
                     /locus_tag="VFF72_05180"
     CDS             10671..>11107
                     /locus_tag="VFF72_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZV92587.1"
                     /translation="MGDLAYLGIADTAKAFRAGSLTPTIVLTQLLDRIARLDSRLHAF
                     LRVDAQPALARAEQATREIASGRWRGPLHGVAIAVKDNIDVAGQATSCHSALMAGHVA
                     ARHAAVVRRLLDAGAIILGKTALHEFATGGPAFDLPWPPARNP"
BASE COUNT         1860 a         3629 c         3640 g         1978 t
ORIGIN      
        1 ggtcgtcaag aagcgatcgg aagagctctt cgccgagttc gagaagcggc tgcgcgcaga
       61 gctcgtcagt gtcgatcaag gccagcttcc gacggccgat gtcgatgcgg ggcagccgcc
      121 gcgatctgtc gagaatgccg cgctcgcgcc tgtcgaagcg aagcgggccc ggggtggcct
      181 caccctctcg gggccacggg cgggcatcgg attggcgctc ggcgtggtcg tggcgctggc
      241 ggccagcgtg ttcggcggat ccccgtggtg gtggttggcg ctgcctgcgt tggcagttgc
      301 cggcggccta cgtcgaagga acgcgtagtg cgcccgcatc cattcgagtt tcatcgggcc
      361 gccggccttg ccgaggcgct tgccctgaag gccagccatg gcgacgaggc ggcgttctac
      421 gctggcggca cggaactgct cgtcgcactc aaggctcggg tgctgcaata cacgcacctc
      481 attgacatca agcggattgc cgagttgcgc gggattcgcc ccggcaagga aggcggcctc
      541 gttatcggcg ccttgtcgac ccaccacggc atctcgaacg atccgctcgt gcgacgcctg
      601 ctgcccgcgt acgccgagct cagcgaacag gtgggaaaca tcagggttcg catcgccggc
      661 accgtcgggg gcgtcctctg ctttgccgag ccgcatgctg acgcgccgac gctgctttgt
      721 gcgctcgacg ctcgcctgcg gctggctggc gccgatggcg aacggacact accgtcacgc
      781 gactttcatc ttggcgaatt cacgacggca cgtcgggacg acgaactgct tgtctcgatc
      841 gaagtaccgc cgcagcccga aggaagccgg gctgcctacc gttgctttgg caaggccgag
      901 cgccccgcgg tcggcgtggc ggccgtctgg cgagaggaag ggccgtcccg gctgagcctt
      961 tgggcgggcg ccatcgtcgg cgcgccgacg cggctggtgc agaccgaaaa ggccgccggc
     1021 gagctgccca caggcttgaa tgccgagcag gtctggcagc gcctgcagcc cgtcgttgcc
     1081 gccgaagctg ctcgactcga tgccagcgac gacttgcacg gaggtgccga ctacaagaag
     1141 cacctcgtcg ccgtcctggc caagcgtgcc atcgaggctt gcctgtcatg agttcccaag
     1201 ccccgaagcc cgcgtcgacc agggtcgatt tcgtcttgaa cggtcatgcc gtgacggtcg
     1261 agatcgagcc cagagagttg ctcatcgacg tcctgcgtga gcggctgcgc ctcaagggaa
     1321 ccaagcgctc gtgcgacgtg caggtctgcg gcgcctgcac cgtcctggtc gacggcctgc
     1381 ccgtatcgag ctgcaccacg ctatgtgtgg atgcacacga gcacaccctc acgaccatcg
     1441 aaggactggc cggcgaaggc cggctggatc cggtgcagcg cgcgttcatt gcgcatggcg
     1501 cactgcaatg cgggttctgc acgcccggaa tgatcctcgc cgtgaggtcg ctgctcagcc
     1561 agcatgcggc tccgtccgac gagacgatcc gccactacct gcgcggaaac atctgccgct
     1621 gcaccggcta cgtcaagatc atggaagcca tacaagatct cgtacgcgat ccgtctcgct
     1681 atcagacgga gcagaaatga tgcgcgacgc caggccggac gagtgcatgc gcgtggtcat
     1741 tgccggcgcc ggcatgggcg gcctgacact ggctctggcg ttgctccaaa aaggcatcga
     1801 cgtcgatgtc tacgagcgat cccctgagct tcgtgaggtc ggcgctggcc tctggatctc
     1861 ggccaacggc agtcgtgtcc tggctgcgct gggtctgcaa ggcgcgattg acgccatcaa
     1921 tctgccgccg gaagaccggg tcgtcaggtt ctggcgcacg ggcgacgagc actccgtcta
     1981 cgtccgcgac aaccgcgaat cggcggtcgg ccgcgtaccg aaggcggacc acactctcgt
     2041 gcaggtcctg cgcgccgagc tccagcgcgt gctctacgag gcagtgtgcg cgctcaagcc
     2101 cgatgcggtg cacttcgatg cgcatgtgct cggcgccgcg aacgtagcct cgggggcgca
     2161 actgctgatc gagggacagg atgcggtcga aggcgcggtg gtggtgggcg ccgacggagc
     2221 acattcgcgc gtgcgccagt cgcagttcgg acccgcgcca gcgcgataca ccggcgcggt
     2281 cgcctggcgc gggctggcgc cgatgaggct gctgaaaccg cagcaccggc gcgctctggc
     2341 ctccacgtgg gtgggtccac gcgcccatgt cactacctac cccgtccggc gcggcacaga
     2401 ggagttcgtc agcttttcgg cgcaggtgga cagcgagacc tggcataccg attcctggtt
     2461 cgagccgggc cagctcgaag acgcgttgcg ggacttcgag ggttggcatc aagacatcct
     2521 cgatctcttc atcaattccg aaagcctctt tcgctggggc ttgttcgtgc gcgatccgct
     2581 ggaggcctgg tcgagaggcc gggtcacgct gctgggcgat gcctgtcact cgatgacgcc
     2641 ctatctgggt atgggggtga acatcacgat ggaggatgcc tgcgtgctcg cccgctgtct
     2701 cgaggccgcg ccggcggaat tcgatcacgc actcaggcgc tacgacgcag cccgcataga
     2761 gcgagccaat cgcacgaagg acgagtcgct gaagatgcac cggatattcc acagccccga
     2821 gctggcgcgt gccgacaccg cgcgttcgta catccagaag caatggtcac cgcaggtggt
     2881 tcgcgaccga tatgactggt tgttgaagta cgacgccacg accgcggaca tctgactcat
     2941 gagcaccatt gtttcgagcc cgaagccacg gccgtgggac ggcatcatcc ccgccgaaga
     3001 gcttgccatc tacgagcggg ccggatgggg cgcgccaggc ggcatgggct gccggccagc
     3061 gatgctcgtc atcgacgtgc agtaccgctc gatgggtgag aggtcgttgc cgatccacga
     3121 ggcaatccgc gaatcggcca ccagctgcgg cgaatacggc tggcgcgccc tgccgcacat
     3181 cgcggcgctg ctggctgtct ttcgcgagat gcgagcgccg gtgctgtacc cgcatgtggc
     3241 cttcaaaggc cctcataacc gcggccagtt cgaggccaag gttccgggcg tcatggatgt
     3301 gccgctcagg ggctacgact ttgtcgccga ggtggcgccg cggaccgaag acatcaccat
     3361 cccgaagttc caggcgagcg cctttcacgc gaccgcgctc accagccacc ttgtcggact
     3421 gggcgtcgac acactcatcg tgaccggctg caccaccagc ggctgtgtcc gcgccaccgt
     3481 cgtcgatgcc tgcgcgttga acttcaaggt cgtggtgccg caagacgccg tctacgaccg
     3541 gtcgcagacc tcacatgcgg tgaatctctt cgacatggcg agcaagtacg ccgacgtgct
     3601 gccgacgagc gaattgatcg agcaattgcg ctcagggccg gtccaatgca ggtagatcgc
     3661 catctttccg tgtcgccgcc gggcgtcccg ctgctctcta tcgatcgcgt caccaagact
     3721 tatgagacgc gtcgcggcga gcaggtcctt gccattggcg aagtgacgct cgatctcgcc
     3781 gaccaggaat tcgtttcgct gatcgggcct tcgggctgcg gcaagtcgac cctgctgcac
     3841 atcgccgccg gtttgctgaa gccgacccgc ggcgacgtcc gcgtgcgcgg cgaaagcagg
     3901 ccgattcacg ccaaagagtt cggcatggtc tttcaggacg cggtgctctt tccctggcgg
     3961 accatccgcg aaaacgtcga gatgccggcc gaggttctgg gactcccaaa ggatgtctac
     4021 aagccgcgcg cccaggaact gctcgagctc gttggccttg cggcttgcgg cgcaatgtac
     4081 ccgcgcgaac tatccggcgg gatgcagcag cgcgcttcga tcgcccggtc gatgatccat
     4141 gacccagagc tcttgctgat ggacgagccg ttcggcgcgg tcgacgccat cacgcgggaa
     4201 aacctgaaca tcgagctgca gcgcatccgc accctggccc gcaagagtgt ccttttcgtc
     4261 acccactcga tcgccgaagc ggtgttcctg tcgaaccgcg tcgtcatcat gagcgccaag
     4321 ccaagccggg tcagcgcggt ggtaaacatc gacctgccgg ccgatcgcgg gtccgaactc
     4381 atggaatcgg tcgagctgaa cgatctcgtg cgaaggatcc accgcgagct cggtggccat
     4441 gcgcatccct agctgcgtga cgtcgatgac tggcgcgagg agactgtcga tgcaaaggga
     4501 aaacgtgctg gagcatgcat tcacgggcgc gctcgttgcc ggattttcga tcttcctcgt
     4561 gatcgcatgg cagctggtca cgagctggca tctgctgcca acctatctgc tgccgacgcc
     4621 tgccgagacg atgcaggcga tctggaagga cctgaccagc gtctcgttct ggacccaaca
     4681 gttcctccag acgcttctcg tcgtgatcgt cggattcgtc atcgccgccg ccgccgggct
     4741 gatcgtcggg ccgctgatca tctcgcaccc gctcgtcgag aggaccttgt atccgttcgt
     4801 cgtcgccttc cagacaatgc ccaagatcgc gatggcgccg ctgctcttgg tctggctcgg
     4861 atacgggcac acgtcgaagt tcctgatcac cgcgctggtc gcattctttc ccgtcgtggt
     4921 caatgcgatt gccggactca aggccggcga cgagagacag ctcatcttga tgcgctcgct
     4981 tcgcgcgagc tggtggacac ggatgacgaa ggtgaggatt ccgaacgcgc tgccctacat
     5041 cttcgccggg ctcgacatcg ccgtggtttt cgccgtcatc ggtgccatcg tcgccgagtt
     5101 cctcggctcg gccaatggcc tgggcagcct cgtcatcgtc aggcagacga atgttgatat
     5161 cgccggtctg ttctccgtcc tgttctttct tgccgtcatg ggatctgcat tgcatttcat
     5221 catcgcaagc ctgggcagga agttcacgtt ctggtcgcaa agcaccgacg ccaagtcggc
     5281 caggcgctga tcgaaagtcg cacaccgttg ttcatcacct tgaaggaagg agtccagaaa
     5341 tgacacgtcc tagcgtcatg aagatgtacg ccgttgccat cggcgctgct atcgcggctt
     5401 tcggcggaac ctcgttcggt gcggatgcgg cggccaattc gtccgcggcc agccaaacgc
     5461 gcgcctggat cagcggcgtg cctgcacccg gcgcgatcaa gcccgcggaa gaaagggacg
     5521 tggtcgtcca gggcgcaagc ccggcgccca acatcggcta cctcgccctc tatgtcgccc
     5581 agaagatggg ctacttcaag gacgaagggc tggacgtcaa gatcaactat tcgcacggcg
     5641 attcggcgcc gcttcaggcc atcggcgcag gccaggcgca gatcatgagt ggcacgcctg
     5701 aagcactgat ccacggctat gaaaggggtt tgcgcggcgt cctcttctac cagacctatg
     5761 acaagctcat cttctcagtg gcggtgctca aggacggcaa gatcaccaag cccgccgaac
     5821 ttgtcggcaa gacaatcggc gtgtcgagca tcgccagcac ctgcgttctc atcgccaagg
     5881 tgctggtgtc ggacgcgggc gcggatccgg ccggcgtgaa gttcctgccc gtcggcaccg
     5941 ggcagcaggc tctcggggcg cttaattcgg ggcaggtcga cgcgctgtgc ctgtgggatg
     6001 ccgcctattc tcagatcgag accggcgcca acaagctgac gctggtgcat tggcagccgg
     6061 cgagcctgac gcacgttggc gatggcggtt atttcaccag ttgggagctg atccaaaagc
     6121 agccgaatat gctggcgcat ttcacccgcg cgatcgccaa ggcgatggtc gcgatccaca
     6181 agaatccttc gggagcgctg cagatctatt gggaggtcaa tccgtcggca aaacccaagg
     6241 gttccgacgc gcaggcgctg cagacaggcc tggagcaact gcagatcgtc ggccgctcgc
     6301 tggacctgag cggcgtgccc aagcctgtcg acgaagccag tctcaatgcc tacgtgaaaa
     6361 ccttcctggc gcagggcgtc attcagaagt cgattccggt cgagcagatc gtcacgaacg
     6421 cgttcgtgcc gatcgccaag gaggccgctg gcgaggcgag caagtgatgc gcctgattcg
     6481 cgcgtcggcg aaaaaggcag cgctcctcgg gaaaaaccta tgaagccgga cgccgagacg
     6541 gagatgctgt ctctctttca cgccgagttg agcttgtgca aggtgtgtcc tggcgagacc
     6601 gtggccgtgc tgaccgcgaa caacgagaag gccgactacg cgcaggcctg catgatcgcg
     6661 gcacagcagc tgggcgccac tgcgtaccag atcaacgtgc cgcgggcgca gacgcgcgcg
     6721 gccggcgtgc agggccgaca tccgctcgcc ggcaatctgc cggcgatcga gtcggccaag
     6781 cgcgccgatt tgctgatcga cctcatgggc ctgctcttct caaaggaaca ggccgagatc
     6841 caggcgtccg gcacgcgggt gctgcgcgtc gtcgagccac tgcacgtcct caagcagatg
     6901 tttccgaccg aggacctgcg ccgacgggtc gagttcgcca agcagttgct cgaatcggcg
     6961 aaggagcttc gcttcacgtc gccggccggc accgacgttc gctattcgct gtcgcagtac
     7021 ccggtgatct gcgaatacgg ctacaccgac cagcccggtc gctgggacca cttcccgtcg
     7081 ggcttttcct tcacccaggc caacgacggc ggcgtcgacg gtatcgtcgt tcttcgcccg
     7141 ggcgacatct tcgcggcgtt caaacgctac gtgcagaccg ctgtccgcct gacgatccgc
     7201 gccggcatca tcgtcgagat cgacggcgag gggatggatg cggcgctgat gcgaagctac
     7261 atcgaaggct tcggcgatcc gcgcggctac gccgtctctc acatcggctg gggcctcaac
     7321 gagcgggcgc agtggcacca gttcgcgacc tcacagcatg tgcaaagcga gtacgtgatg
     7381 aacgcgcttt ctttctacgg caacgtgctc ttctcgaccg gccccaatac cgaactcggc
     7441 ggtagcaacg acacgccgtg ccatctcgat ctgccgatga ggggctgcag cctgtggctt
     7501 gatggcgtgc cgatcctcga agacggccgg gttgtccatc cgcagatgaa ggtgcctggg
     7561 cgatgagtgg cgctgaaagg tcgacgacat cggcccgctc gccggtcggg tggcagtgcg
     7621 cgttcgggaa ggcccatggc tgaatccggg gcgccggtgt tgatcgccgg ggccggcctt
     7681 ggcggcctgg ccgcaggcct ggccctcttg cgaaagggca ttgatgtcga gattttcgag
     7741 cagtcggcga gcctgggcga ggtcggcgcg ggagtgcaga tctcggccaa cggaacgacc
     7801 gtgctcttcg cgcttggtct tgaggaggac gtgcgccgcg acggtgtgcc ggtgactgac
     7861 aaggagattc gtctgtggaa ctccgggcag tcttggtcac tcttcaagtc cgaccccttg
     7921 ccagcgggcg ggcgctatga gcatccgatg ttcaccctgc atcgcaagga cctgcacgcg
     7981 atgctggtgc gggcggtgcg cgagctgaag gctgacgcga ttcacctcgg ccgccgctgt
     8041 gtccgtttcg aagcaacgga tgcggacgtc aggctggcct ttgagcaggg accgccggcc
     8101 gcgggctcga tcctgatcgg tgccgacggc ctccattcgc gcatccgcga gcagctttgc
     8161 ggccccatgg acgcggagtt cacgggccag ctcgcttggc gcggcatggt gccgcaggat
     8221 cgattgacgc cgcagatgcg tcgtctggtc ggtacgaact gggtcggccc gagcgcgcac
     8281 gtcacctgct atccggtgca gcgcggcgag gtcttcaatt tcgtcggcca ggtcgaccgc
     8341 accgattggc gggtcgagtc gtgggtcacc gaaggctcga ctgaggagtg cctggccgac
     8401 ttctcgggat ggcatccgca ggtgcggcac atgatcgagt cggcgcccaa gctattcaaa
     8461 tgggcactct tcctgcgtca gcccctgccg cgctggagcg tcggccgcgt aaccctcctg
     8521 ggcgatgcct gccacgcgat gcttccttat ctcgggcagg gcgccaacat ggcgcttgaa
     8581 gacgggttcg tccttgcccg ctgcctggcg gccgcatcga ccgatccgca agtcgctctc
     8641 ttgcgctacg aaaacgcgcg ccgcgagcga accgagaaga tcgtccgcgc ttcggcggag
     8701 atggcgcgca ccttccacaa cccggcgctg gccgaggccg ctagcgcgca gcgctatgtc
     8761 gagacgcagt ggagtggcgg catggtgcgc acgcgctacg actggatcta tggctacaac
     8821 gcctcgacct cgccgatctg aacgaatcgg atttcaacca ggagcaatca gtccatgaaa
     8881 gctgtctttc gttgtgcgtg gctcatctgt ctcgcgatgg tggcgccatt ggcggtggcc
     8941 caggcttatc cggagcatgc cgtgaagctg gtcgtgccct tcccggcagg ggccaccatg
     9001 gacgccgtcg ttcgtctgat cggcgagaag ctcggcagcc gatgggggca ggccgtcgtc
     9061 gtagaaaacc gcaccggcgc cgccgggctg attggcaccg cttcggtcgc caaggccgtg
     9121 cccgatggct acaccatcct ggccgtcgcc aatagcttcg ccgccaatcc cacactgcgc
     9181 agcagcatgc cctacgacac ggcgaaggat ttcgtgccga tcacgctgat cggctcgacg
     9241 ccgctcgtgc tggtcgccaa cccgcacctg cccgcgagct cgacggccga gctcgtcgcg
     9301 ttggcgaaaa aggagccggg ccgcattccc tacggtgcgg cggccggcgc atcgccgcac
     9361 ttggcgatgg cttggctcga gtcggtggcg gggatcaagc ttctcttcgt gccgtatcga
     9421 ggccagtcgc aggcgcagac cgacctgctc ggcgggcaag tcaagctcgt cttcggcaat
     9481 ctcccggaca ttctgccgct ggtcaaggac ggtcagctga gggcactcgg catcgcgacg
     9541 ggcacgcgct cgccgctggc tcccgacatt ccgaccctcg ccgaggcagg ctacaaaggc
     9601 ccgcagtggg actcctggta cggattcgtt gccccggtcg ggacgcccgc tgccatcgtc
     9661 gacaagttcg ctgccgacgt ccgtgcagtt ctcgcgatgc ccgacgtgaa ggaggcgctg
     9721 cgcagccggg gcatcgtcgc tgtcggcgac acgccggcgg agttcgacgc cttcctcaaa
     9781 cgcacggctg acgactacgc gaagatcatc cggcaagcgc acatccatgt cgattgagcc
     9841 tcgcggccaa agctacggag cgcacgtccg ggcgaacgaa atccgccagc actacctgag
     9901 atttccgggc gccggccagg cgctgatcgt cgttcctggc attacgagcc cggccgcaac
     9961 ctgggatttc gtcggcgagc gactcggccg catcttcgac acctacgtgc tcgacgtgcg
    10021 cgggcgcggc ctgtcgcagg gcggcagcga cctgtcgtat gacctcgaca cctgcgccag
    10081 cgacgtggag cagttcgcaa gcgcgctcgg actgcgtgac ttcgccttgc ttggccattc
    10141 gatgggagga cgcatcgcgt tgaggctcgc gggccgcggc ggcaccgcga tttcgcgcct
    10201 cgtgctcgcc gacccgccac tgagcgggcc tggccgcagg ccctacggcc gctcgctcga
    10261 cttctatctc gaggcgatcc gtagggcacg cgccggcacg ctggacgcag aagccgttcg
    10321 gcaaacctat ccgcgttgga gcgacgcgca actgcgttca cgggcgcagt ggctgcatac
    10381 ctgcgacgag acggcggtcg cggccagcgt gcgcggcttc caggaagacg acatccacgt
    10441 cgacttcgca cgcctgcacc tgccgacgct gctgctcgcg gccggatcgg gcggcgtcgt
    10501 ctcagacgaa gacgccgccg aggtgctgcg gctcatgcct tcgctcgaga ttcgccggct
    10561 ggcatcggcc ggccacatgc tgcccttcga cgatctcgag ggcttcctcg ccatcgcagg
    10621 cgaatttctg aagcgcaatt gagaattggt ggcgattcgg ggacgatgca atgggtgatc
    10681 tcgcctatct cggcatcgct gatacggcca aggcatttcg cgcgggaagc ctgacgccga
    10741 cgatcgtcct gacgcagctt ctcgaccgga tcgcgcgcct cgacagtcgc ctgcatgcgt
    10801 tcctgcgcgt cgatgcgcag ccggccctgg ctcgcgcgga gcaggccacg cgggagatcg
    10861 cgtccggccg ctggcgcggg ccgctgcatg gcgtcgccat tgcggtcaaa gacaacatcg
    10921 atgtcgccgg ccaggcaacg agctgtcact cggccctcat ggccggccat gtcgccgcgc
    10981 ggcatgccgc cgtcgtccgt cgtcttctcg atgccggcgc gatcatcctt ggcaagacag
    11041 cgctgcacga gttcgctacc ggcggcccgg ctttcgatct tccttggccg ccggcgcgca
    11101 acccctg
//
LOCUS       DASRWS010000282         7100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1559845, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000282 DASRWS010000000
VERSION     DASRWS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559845"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFF72_05185"
     CDS             complement(<1..411)
                     /locus_tag="VFF72_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZV92588.1"
                     /translation="MSSPGAVQWARLFDLAEQPSLPLQARLRLAVVGAILEGHLGAGA
                     PLPSSRELSSLLGISRNTVTAAYVQLVDEGFLEARPRSGVFVAVGARPTDPAAAEPLS
                     NRQPVGLAPNWTERVRRSLTGRPTLAKPVRWQDYP"
     gene            617..985
                     /locus_tag="VFF72_05190"
     CDS             617..985
                     /locus_tag="VFF72_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZV92589.1"
                     /translation="MLAEFSAAWNRHDIDALMSFMHDDCVFEAAGGNEACGARHVGRE
                     AVKKAFAAAWSTVHDAQWTRGRHFVHGDFGVSQWTFTGTAADGSRIETDGVDVFTFKD
                     GRILVKNAFRKARPNLPAAR"
     gene            996..2441
                     /locus_tag="VFF72_05195"
     CDS             996..2441
                     /locus_tag="VFF72_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZV92590.1"
                     /translation="MADFATSPAAARVAAGPQQRYDPRYDPLVAADPGTGRAYAPSYW
                     VSTAGDPPEDDGPVHGDMDADVVVIGSGSTGISTAVYLAQEHGVRAVVLEANQASWGC
                     SSRSGGQGQNASGRLKRSQWIARWGLDVARRLDTEIRTGFDNFKALTREIDCNAWDGG
                     HLYLAHRADRLAGLAREAALMRDTFGYATRMMDIAEVRRDYCDEREAAGAMFEPEGVG
                     IQPLKFTFGMIRKARSLGARIHPASPVQGWRSEGGVHHLRTPGGTVRARRVAVCTGGY
                     TSQGLNPLLRNKIMPILSNSLATRPLTEAELAATNFRSQTFLTDTRTLRFYYRLLPDN
                     RLQIGSRSAVSGADAEDPVHLKLLTDAIARKFPPLAGIEIDYSWWGWVDVSHDMMPRI
                     VRPDPAQAIWYALGYGGNGVSFSTWAGKRLAERIVGKDAGRDVFTLPIYDSPLEYPNV
                     MGAVRSELFAPFRRFGQHLLYRWYWLNDEKL"
     gene            2498..4099
                     /locus_tag="VFF72_05200"
     CDS             2498..4099
                     /locus_tag="VFF72_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92591.1"
                     /translation="MTSPILTRRDALAAAGAMLAAAGLPLDALAVEDVKKGGTLVIGS
                     TQVPRHLNGAVQSGVATAMPGTQIFASPLRFDDKWQPQPYLAESWKLADDGKSLTLNL
                     RKNALFHDGKPVTSADVAFTVMAIKANHPFKTMMGPVEKVDTPDAHTAIIRMATPHPA
                     IVLCMSPALCPILPKHIYDDGQDLKNHPRNSKDVIGSGPFKVVEFTPSQRTVMEKFDK
                     FFLPGKPYLDKIVININPDGSTLMLGFERGDLQLYPFVSLPTDLKRVSANKNATLTSR
                     GYEGIGPINWMAFNLAKKPLSDLAVRKAIAMSIDKKFITKVLMGGFAVVDDGPIVPSS
                     PFAVTDVVLYPLDLKKAAEMLDGAGYKAGANGERFKLTIDYLPGQDDQQKNVAEYVRG
                     QLKKVGIAVEVRASADFPSWAKRMATHDFDLGMDLVFNWGDPVIGVARTYLSTNIKPI
                     VWTNTQSYNNPKVDELLEAAGTQLDPTKRKALYATFEKIVTDELPIDFINVIPYHTAA
                     SKRVGNLPTTIWGPMSPMDEVYLKT"
     gene            4106..5077
                     /locus_tag="VFF72_05205"
     CDS             4106..5077
                     /locus_tag="VFF72_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92592.1"
                     /translation="MKFARLFVLRLLQGALLVLAVVVLNFILVHAAPGDPVETIAGAS
                     GGMSPELMAQLRTQYGLDRPLVVQLGVYLAKVLHGNFGYSYYFNLPVTSLILERVPAT
                     LLLVLCAVILAFAVGTTLGVLAARRPNGVLSQFITVLSLVGFATPVFWMGIMLIILFA
                     SIWPILPVSGMRSVDAIGGGWRDVVDIAHHLVLPTVTLALVYLAQYSRLSRSSMLDVL
                     GADFIRTARAKGLPERVVLYKHALRNALLPVVTVLGLQFGNVLAGAILVETVFNWPGL
                     GRLAFDSVLRRDYPTILGVLVFASIVVIVMNLLTDLAYRLIDPRIKV"
     gene            5184..6080
                     /locus_tag="VFF72_05210"
     CDS             5184..6080
                     /locus_tag="VFF72_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92593.1"
                     /translation="MSAVLPPHAVPTASPPLKVEHPTVEALRMFMRNPSAIAGMVLML
                     AIVLVAVVGPWLYPADPFEIKAAPLTPPFGEDAWLGSDYLGRDVLTMLVYGGRATLLV
                     GAVAALLSVAIGIGIGAFAGYYGGRVDAWLMRVTEFFQVLPALLFAMVIVTLFGPTIV
                     HVTIAIGVVSWTATARLTRAEFLKVKGLEFIRAERAIGARHARIIWKVILPNALPPLV
                     VSATLAVGAAILFDAGLSFLGLGDPNQMSWGLMIGSSREYVLSCWWGVAFPGIAIFLT
                     VLAVSLIGDGLNDALNPKLRER"
     gene            6080..>7100
                     /locus_tag="VFF72_05215"
     CDS             6080..>7100
                     /locus_tag="VFF72_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92594.1"
                     /translation="MTAEAALLVVDDLRVEFRTRRGRALVLGGVEFELRGGETLCVVG
                     ESGCGKSMTALAILGLVPSPPGRVTGGRIVFQGEDLVQASDARLRDVRGNRISMIFQE
                     PMTSLNPVFSVGEQIAEALRLHAGLDGRAARARAIEMLQQVGIPAPERRVDEYPHQLS
                     GGMRQRVMIAMALACRPDILIADEPTTALDVTVQAQIFDLLRGLQREKGTAILLITHD
                     MGAVAEMADRVVVMYAGRIIEHGSAAQVLQAPAHPYTQGLIDCLPELGSSVAEAGGGK
                     PAPLAEIAGVVPSLWELASGCAFRERCTKAMPRCAADVPPMLAVGSADGHGAACWLLE
                     GTTPAA"
BASE COUNT         1064 a         2535 c         2396 g         1105 t
ORIGIN      
        1 ggggtagtcc tgccaacgga ccggcttggc gagcgtcggc cgtccggtga gcgaacggcg
       61 gacgcgctcc gtccagttcg gcgccaggcc gaccggctgc cggttcgaga gcggctcggc
      121 cgcggcgggg tcggtcggac gggcgccgac ggcgacgaaa acgccgctgc gcggccgcgc
      181 ctcgagaaac ccttcgtcga cgagctgcac gtaggccgcg gtcacggtgt tgcggctgat
      241 gccgagcagc gacgaaagct cgcgcgacga cggcagcggc gcgccggcgc cgaggtggcc
      301 ttcgaggatg gcgccgacga cggcaaggcg aagccgggcc tgcagcggca gcgacggctg
      361 ctcggcgagg tcgaagaggc gtgcccactg cacggcgccg ggcgaggaca tggcagcaag
      421 tctagtggcc tcgccgtggc cggcactcgg ccggcgaacg tcgcgaatcg cgccgtcgtc
      481 gcggaggtgc ggcatcgcgc tggctcgtcc gatcggctcg atctggcact accggggtcg
      541 cgacggcgcg cctacgctcg cggcgaaccg aggagcaaac gacttgagcg caacccgccc
      601 ccccgtcacc gaggccatgc tggccgaatt cagcgccgcc tggaaccgtc acgacatcga
      661 cgccctgatg tccttcatgc acgacgactg cgtcttcgag gcggccggag gcaacgaggc
      721 ttgcggcgcc cgccacgtcg gccgagaggc ggtgaagaag gcgttcgccg ccgcgtggtc
      781 gacggtgcat gatgcgcagt ggacgcgcgg acggcacttc gtgcatggcg acttcggcgt
      841 ctcgcaatgg accttcaccg gcaccgcggc ggacggctcg cgcatcgaga ccgacggcgt
      901 cgacgtcttc acgttcaagg acggccggat cctcgtcaag aacgcctttc gcaaggcgcg
      961 cccgaacctg cccgcggcac gctgacgcga cgacgatggc cgacttcgcg acatccccgg
     1021 ccgcggcgcg cgtcgcggcc ggcccgcagc agcgctacga cccgcgctac gacccgctcg
     1081 tcgctgccga tccgggcacc ggtcgcgcgt acgccccgag ctactgggtg tcgacggcgg
     1141 gcgacccgcc cgaagacgac ggccccgtgc acggcgacat ggacgccgat gtggtcgtca
     1201 tcggctcggg ctcgaccggc atctcgaccg cggtctacct ggcgcaggag cacggtgtgc
     1261 gggccgtcgt cctcgaggcc aaccaggcgt cgtggggatg ctcgagccgc agcggcggcc
     1321 agggccagaa cgccagcggc cgcctgaagc gctcgcaatg gatcgcccgc tgggggctcg
     1381 acgtcgccag gcggctcgac accgagatcc gcaccggctt cgacaacttc aaggcactga
     1441 cgcgcgagat cgattgcaac gcctgggacg gcggccacct ctacctcgcg caccgcgccg
     1501 atcgactcgc cgggctggcc cgcgaggcgg cgctgatgcg cgacaccttc ggctacgcga
     1561 cccggatgat ggacatcgcc gaggtgcgcc gcgactactg cgacgagcgc gaggccgcgg
     1621 gcgcgatgtt cgagcccgag ggcgtcggca tccagccgct gaaattcacc ttcggcatga
     1681 tcaggaaggc gcgctcgctg ggtgcgcgca tccatccggc cagcccggtg caaggctggc
     1741 gcagcgaggg cggcgtgcat cacctgcgaa cacccggcgg caccgtgcgg gcgcggcgcg
     1801 tcgccgtctg caccggcgga tacacctcgc aggggctcaa tccgctgctg cgcaacaaga
     1861 tcatgccgat cctgtcgaac tcgctggcga cgcggccgct caccgaggcc gagctggccg
     1921 ccaccaactt ccgttcgcag accttcctca ccgacacgcg tacgctgcgc ttctactacc
     1981 ggctgctgcc cgacaaccgg ctgcagatcg gcagccgcag cgccgtcagc ggcgccgatg
     2041 ccgaggatcc ggtgcacctg aagctgctca ccgacgcgat cgcccgcaag ttcccgccgc
     2101 tcgccggcat cgagatcgac tactcgtggt ggggctgggt cgacgtcagc cacgacatga
     2161 tgccgcgcat cgtccgcccg gatccggcgc aggcgatctg gtacgcgctc ggctacggcg
     2221 gcaatggcgt ctcgttctcg acctgggccg gcaagcgcct ggccgagcgc atcgtcggca
     2281 aggacgcagg ccgcgacgtg ttcacgctgc ccatctacga ttcgccgctc gagtatccga
     2341 acgtcatggg cgcggtacgc tcggaactgt tcgcgccgtt tcgccgcttc ggccagcacc
     2401 tgctctaccg ctggtactgg ctcaacgacg agaagctgtg aagccggtgc gctcgcgcag
     2461 cgataccctt actcatcaca acgacggaga cgaccccatg acctcgccca tcctgacgcg
     2521 tcgcgacgcg cttgccgccg ccggcgccat gctggccgcc gccggattgc cactcgacgc
     2581 cctggccgtc gaggacgtga agaagggtgg cacgctcgtc atcggcagca cgcaggtgcc
     2641 gcgccacctc aacggcgcgg tccagtcggg cgtcgccacg gcgatgcctg gcacgcagat
     2701 cttcgccagc ccgctgcgct tcgacgacaa gtggcagcct caaccctacc tggccgagtc
     2761 gtggaagctc gccgacgacg gcaagtcgct gaccctcaac ctgcgcaaga acgccctctt
     2821 ccacgacggc aagccggtga cctcggccga cgtcgccttc acggtcatgg cgatcaaggc
     2881 caaccatccg ttcaagacga tgatgggccc ggtcgagaag gtcgacacgc ccgatgcgca
     2941 cacggcgatc atccgcatgg cgacgccgca cccggcgatc gtgctgtgca tgagcccggc
     3001 cctctgcccg atcctgccca agcacatcta cgacgacggc caggacctca agaaccaccc
     3061 gcgcaacagc aaggacgtga tcgggtcagg gccgttcaag gtcgtcgagt tcacgccgtc
     3121 gcagcgcacg gtcatggaga agttcgacaa gttcttcctg ccgggcaagc cttacctcga
     3181 caagatcgtc atcaacatca atccggacgg gtcgaccttg atgctcggct tcgagcgcgg
     3241 cgacttgcag ctgtatccgt tcgtctcctt gccgaccgac ctcaagcgtg tctcggccaa
     3301 caagaatgcg acgcttacgt cgcgcggtta cgaaggcatc ggcccgatca actggatggc
     3361 cttcaacctc gccaagaagc cgctctccga cctggcggtg cgcaaggcga tcgccatgtc
     3421 gatcgacaag aagttcatca cgaaggtgct gatgggcggc ttcgccgtcg tcgacgacgg
     3481 cccgatcgtg ccgagcagcc cgttcgcggt gaccgacgtg gtcctctacc cgctcgattt
     3541 gaagaaggcc gccgagatgc tcgacggcgc cggctacaag gcgggcgcca acggcgagcg
     3601 cttcaagctc accatcgact acctgccggg gcaggacgac cagcagaaga acgtcgccga
     3661 gtacgtgcgc gggcagctga agaaggtcgg catcgccgtc gaggtgcgcg cctcggccga
     3721 cttcccgtcc tgggccaagc gcatggcgac gcacgacttc gacctcggca tggacctcgt
     3781 cttcaactgg ggcgacccgg tgatcggcgt cgcccgcacc tacctgtcga ccaacatcaa
     3841 gccgatcgtc tggaccaaca cccagtcgta caacaacccg aaggtcgacg agctgctcga
     3901 agcggccggc acccagctcg acccgaccaa gcgcaaggcg ctctacgcga ccttcgagaa
     3961 gatcgtcacc gacgagctgc cgatcgactt catcaacgtc atcccgtatc acacggcggc
     4021 gagcaagcgg gtcggcaact tgccgacgac gatctggggg ccgatgtcgc cgatggacga
     4081 ggtctacctc aagacctgag gcgtcgtgaa gttcgcccgc ctgttcgtcc tgcgcctgct
     4141 ccagggcgcg ctgctggtgc tggcggtcgt cgtcctcaac ttcatcctcg ttcacgcagc
     4201 gccgggcgat ccggtcgaga ccatcgccgg tgcgagcggc ggcatgagcc ccgagctgat
     4261 ggcgcagctg cgcacgcagt acgggctcga ccggccgctc gtcgtccagc tcggcgtcta
     4321 cctcgccaag gtgctgcacg gcaacttcgg ctattcgtac tacttcaacc tgccggtcac
     4381 ctcgctcatc ctcgagcgcg tgccggcgac gctgctgctc gtgctgtgcg cggtgatcct
     4441 cgccttcgcc gtcggcacga cgctgggcgt gctcgcggcg cggcgtccca acggcgtgct
     4501 gtcgcagttc atcacggtgc tgagcctggt cggcttcgcc acgccggtct tctggatggg
     4561 catcatgctc atcatcctgt tcgcctcgat ctggccgatc ctgccggtct ccggcatgcg
     4621 cagcgtcgat gcgatcggcg gcggctggcg cgacgtcgtc gacatcgccc accacctcgt
     4681 gctgccgacg gtgacgctgg cgctcgtcta cctggcccag tacagccggc tctcccgctc
     4741 gtcgatgctc gacgtgcttg gcgccgattt catccgcacc gcgcgcgcca aggggctgcc
     4801 cgagcgggtc gtcctctaca agcacgcgct gcgcaacgcg ctgctgccgg tggtgacggt
     4861 gctgggcctg cagttcggca acgtgctggc cggcgcgatc ctggtcgaga cggtgttcaa
     4921 ctggccgggc ctgggccgtc tcgccttcga ctcggtgctg cgccgcgact atccgaccat
     4981 cctcggcgtg ctcgtcttcg cctcgatcgt cgtcatcgtc atgaacctgc tgaccgacct
     5041 cgcgtaccgc ctgatcgatc cgcgcatcaa ggtctagatg aagcccccac gctcgcgttc
     5101 gctcgctgcc ccccgagggg gcgcccgccg atggaccggc gaagccggat ccatggcggt
     5161 ggcttggttg ggctggcact gacatgagtg ctgtgctgcc gccccacgcg gtgccgacgg
     5221 cctcgccgcc gctcaaggtc gagcatccga ccgtcgaggc gctgcgcatg ttcatgcgca
     5281 acccgtcggc gatcgccggg atggtgctga tgctggcaat cgtcctcgtc gccgtcgtcg
     5341 ggccgtggct gtatccggcc gaccctttcg agatcaaggc ggcgccgctg acgccgccct
     5401 tcggcgagga cgcctggctc ggcagcgact acctcggccg cgacgtgctg accatgctcg
     5461 tctacggcgg ccgggcgacg ctcctggtcg gcgccgtcgc ggcgctgctc tcggtggcga
     5521 tcggcatcgg catcggcgcc ttcgccggct actacggcgg ccgcgtcgac gcctggctga
     5581 tgcgtgtcac cgagttcttc caggtcttgc cggcgctcct cttcgcgatg gtcatcgtca
     5641 ccctgttcgg gccgacgatc gtgcatgtga cgatcgccat cggcgtcgtc agctggacgg
     5701 ccacggcccg gctgacgcgg gccgagttcc tcaaggtcaa ggggctcgag ttcatccgcg
     5761 ccgagcgcgc gatcggcgcc cgccatgcgc gcatcatctg gaaggtgatc ctgcccaacg
     5821 cgctgccgcc cctggtcgtc tcggcgacgc tcgccgtcgg cgcggccatc ctcttcgacg
     5881 ccggcctgtc gttcctcggc ctgggcgatc cgaaccagat gagctggggg ctgatgatcg
     5941 gctcgagccg cgagtacgtg ctgtcgtgct ggtggggcgt cgcctttccg ggcatcgcca
     6001 tcttcctcac cgtgctggcg gtgagcctga tcggcgacgg cctcaacgac gccctcaatc
     6061 cgaagctgcg cgagcgctga tgaccgccga ggccgccttg ctcgtcgtcg acgacctgcg
     6121 cgtcgagttc cggacgcggc gcggccgcgc gctggtgctg ggcggcgtcg agttcgagct
     6181 gcgcggcggc gagacgctgt gcgtcgtcgg cgaatcgggc tgcggcaaga gcatgacggc
     6241 actcgccatc ctcggcctgg tgccgtcgcc gcccggccgt gtcaccggcg gtcgcatcgt
     6301 cttccagggc gaagacctgg tccaggcgag cgatgcccgg ctgcgcgacg tgcgcggcaa
     6361 ccgcatctcg atgatcttcc aggagccgat gacctcgctc aacccggtct tcagcgtcgg
     6421 cgaacagatc gccgaggcgc tgcgcctgca cgccggcctc gatggccggg cggcgcgggc
     6481 gcgggcgatc gagatgttgc agcaggtcgg catcccggcg ccggagcggc gcgtcgacga
     6541 gtacccgcac cagctctcgg gcgggatgcg ccagcgcgtc atgatcgcga tggcgctggc
     6601 ctgccggccc gacatcctga tcgccgacga gccgaccacg gccctcgacg tcacggtgca
     6661 ggcgcagatc ttcgacctgc tgcgcggcct gcagcgcgag aagggcacgg cgatcctgct
     6721 catcacccac gacatgggcg ccgtcgccga gatggccgat cgcgtcgtcg tgatgtacgc
     6781 ggggcgcatc atcgagcacg gcagcgccgc gcaggtgctg caggcgccgg ctcatccgta
     6841 tacgcagggc ctcatcgact gcctgcccga gctcggcagc agcgtggccg aggccggcgg
     6901 cgggaagccg gcgccgctgg ccgagatcgc aggcgtcgtg ccttcgctgt gggagctggc
     6961 cagcggctgc gcctttcgcg agcgttgcac gaaggcgatg ccgcgctgtg ccgctgacgt
     7021 tccgccgatg ctggcggtgg gcagcgccga tggccacggc gcggcctgct ggctgctcga
     7081 agggacgacg ccggccgcgg
//
LOCUS       DASRWS010000283        10889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_422022, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000283 DASRWS010000000
VERSION     DASRWS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422022"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFF72_05220"
     CDS             <1..1398
                     /locus_tag="VFF72_05220"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZV92595.1"
                     /translation="RYPGVRADSDMYTLAYSFRPWRQPRAIVDGATILSYLQDTAREN
                     GIEERIRYGHRVTGAAWSTADARWTVEARCAESDQVVRFSCNFLFACSGYYRYAAGHA
                     PEIAGRDHYRGRVVHPQHWSDDIDHAGRRVAVIGSGATAMSLVPAIAATASHVTMVQR
                     SPTWVISQPADDRLARRLARFLPDRLAYTAVRWQRVLMGVFFFALCRGRPALASRLLT
                     AGVRARVGPQFDIATHFAPRYKPWEQRLCLVPDGDFFRTLRSGRASIVTSEIERFTET
                     GLRLRSGDLVDADLVVMATGLELELLGGIAVSVDGRVVDWASKLTYKGMMYDGVPNFA
                     SVFGYANASWTLRSDLISGYVCRLLQRMNRRGLRQCTPRNSDPGITTAPWLDLSSGYI
                     RRAAERLPKQGSRAPWSLCQNYLRDLAPMRWSRLDDGAMVFSNPGGRPPVASAPGCGK
                     APTKIVGSPLRDRSP"
     gene            1395..2489
                     /gene="ychF"
                     /locus_tag="VFF72_05225"
     CDS             1395..2489
                     /gene="ychF"
                     /locus_tag="VFF72_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZV92596.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDPRLARLAEIVKPERIVPAIVEFVDIAGLVAGASKGEGLGNQFLAHVRETDAIVN
                     VVRCFDDENVIHVAGRVDPVADIEVIQTELCLADLATVEKSLARQLKAAKSGNDKEAK
                     RLVDVLEKCNATLDQARPVRSIDFSKEELAVLKPLCLITAKPAMFVANVAENGFADNP
                     HLDRLREVASRQHAPVVAISAKTEAELADMDDADRQMFLAEMGQTESGLARLVRAAFT
                     LLGLQTYFTAGPKEVRAWTIHAGDTAPQAAGVIHTDFERGFIRAQTVAYEDFMAYGGE
                     HGARDAGRMRAEGKDYVVKDGDVLNFLFNV"
     gene            2526..5783
                     /locus_tag="VFF72_05230"
     CDS             2526..5783
                     /locus_tag="VFF72_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZV92597.1"
                     /translation="MTQTAEPPPRHTPASSWQLELPAFAATVVAYVAAGLVALLLTIA
                     PAGTSPLYPAAGIALASVLVQGWRMLGAVALGSLSVNLISLSLHGAGDHRVATAAVVA
                     TGAVLQAALGAALIRRYVRQPLVLTLPADVARFMAACLGASLVAPTIGALALSLFSAL
                     PPGGAVWHWTTSWIGDLAGLLIVTPIALTIFGRPASEWRPRRVSVGVTLSVVVVFLAL
                     GIVQLGRWNDERVREAFEHDASSASLMLATQMQEPLRALEALHDVFTVSHQIGGAEVR
                     RISQHWLNGTSVRAIGWSERVHHDDIAAFEARARADGALGYRIYDRPAVPGARPVAPV
                     ASLESDLGLGLRGDGGDVIAIRFVEPSEMNGAALGLNAVSIPTGGAAALIAAVDSGAP
                     AVSTGFQMTPEGDGEAQPGVLLYQAVYDGDPVPPGQRRSAFRGLVFVVSSLDRQLASM
                     AGRVPAYLDLCVVDATPGGSPRRLGGRPGCDTGTPELLHDRPIVFAGHPWDLRISARP
                     DAVPDANDRSAWIFSAAGLLSAALLGASLLITTGRTRRIESAVRDRTAALRAEVGERH
                     AAEAALRASEERFRNILDHVPIGIVYAGLDGRMIQANPRFCELTGYSEAELVALSPAD
                     LTDPDDLAHDQALTAELVSEKIPMYRRHRRYITKKGDVVWVRATVTLLRDSAQQPWRI
                     VGVVEDITEHLRLEEAERAREAAEAANVAKSEFLSRMSHELRTPLNAMLGFAQLLEID
                     KRQPLSPVQRPWVAQIQQAGWHLLEMINDVLDLSRIESGNLRLQITALELPELVAATV
                     ALVGSEAVRRNVRISQDLGPGTAGILGDPTRVKQILTNLLSNAVKYNVDGGRVHVASR
                     LTAADRVEISVTDTGMGMTAEQMSGLFKPFNRLGRERSALQGTGIGLVISRRLAELMG
                     GSITVRSLPGEGSSFTLRLPKSIDPDTVPSPLDALETGPAEYHRRIVHYIEDNETNVE
                     VMRGILSQRPQVQMEVSVTGLDGLAAVRARRPHLILLDMHLPDINGLELLRHLKSDPG
                     TAGVPVMIVSADALAPQIDAALAAGASRYLTKPVDVAELLGALDDLLDRMDTGFS"
     gene            5917..6843
                     /gene="rpoH"
                     /locus_tag="VFF72_05235"
     CDS             5917..6843
                     /gene="rpoH"
                     /locus_tag="VFF72_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HZV92598.1"
                     /translation="MNHSPAATALTVRDPWALVPSLGNLDAYISAINRIPLLTAEEES
                     RFARELRATGDLGAAGKLVLSHLRLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRF
                     DPDQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQSLKDDAV
                     AGDTHRSTLTQSEVDMLAGTLNVKREEVLEMETRLSGGDVALEPQNDDGEESYAPIAY
                     LADDNSEPTHVLESRKRDWLAADGISLALESLDPRSRRIVEERWLKVNDDSSGGMTLH
                     DLAGEYGVSAERIRQIEVAAMKKMKKALAHVT"
     gene            complement(6923..7534)
                     /locus_tag="VFF72_05240"
     CDS             complement(6923..7534)
                     /locus_tag="VFF72_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZV92599.1"
                     /translation="MTLSRRCLIGAAGLAILPLPGCDRLLGRSSEPRFHFTDITGAEF
                     ARRLELPDADGRTRSLADWKGKVIVVFFGYTQCPDFCPTTLTELAQVKKDLGAAGRRV
                     EVLFVTVDPERDTPQVMKSYVSNFGPDVVGLRGSPEQTAEAAKEFKVFYAKVPGKTPG
                     SYTMDHSAASFVFDTQGKVRLYVPYGADQKAFEQDLSQLLSAA"
     gene            complement(7531..8448)
                     /gene="cyoE"
                     /locus_tag="VFF72_05245"
     CDS             complement(7531..8448)
                     /gene="cyoE"
                     /locus_tag="VFF72_05245"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZV92600.1"
                     /translation="MSDTLASAAPQRASLPSRARQFYALTKPRVVQLIVFCAAIGMFL
                     ATPDLPAWRKAVPAVIGIWLVAAAAAAFNCLVEQRIDSRMARTSWRPTATGELTTRQA
                     LAFSVALCIGGSTLLWLFVNPLTMWLTFATFVGYAVVYTVLLKPMTPQNIVIGGASGA
                     MPPVLGWAAIRGDVGAEALILCLIIFLWTPPHFWALALYRAEDYRRAGLPMLPLTHGS
                     EFTRLHVFLYTIVLFAATLLPFVAGMSGPIYLAAAIVLGVMFCGYAWRLWRHYSDALA
                     RRTFRFSIVHLSLLFAALMVDHYLWPMLA"
     gene            complement(8468..9622)
                     /locus_tag="VFF72_05250"
     CDS             complement(8468..9622)
                     /locus_tag="VFF72_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZV92601.1"
                     /translation="MSAAVPAYDLAPVFAILATGLVLASGPLIWFWRRRRGDATGARL
                     RALALLALFLSFDLVILGAFTRLSDSGLGCPDWPGCYGHASPVGARAPINAAEAAVPT
                     GAVTHTKAWIEMAHRYSATAIGVLVIALAILAVVERRRGRLVASPRWAVATVLWIIAQ
                     GAFGALTVTMSLYPAIVVLHLVGGLGLLAMLALQAELYEPRPLPLGRTLRVGLAVVAA
                     LALVQIILGGWVSTNYAVLACRDFPTCQGQWWPDMNFAEGFVVLRPLGISGEGGFLPF
                     QALTAIHMAHRIGALVVLPMLLWLAARLAAAGDPQARRWGGWLAGIAAWQLASGLGNV
                     LLGWPLLTALAHTAGSAALVVMLTTLLARSRPLRQRVESPWPSRAQHVAP"
     gene            complement(9619..10323)
                     /locus_tag="VFF72_05255"
     CDS             complement(9619..10323)
                     /locus_tag="VFF72_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92602.1"
                     /translation="MTTDPPLLNLSVHSLPDPQAGPDAASRTRRGRWTMIVVLLICAA
                     PVVASYLAFYFDLRPSTLTNYSDLIVPPRPLPADLPLRDLRGRAVAAPALQGQWLLVV
                     VSGGACDANCEHRLWLQRQLHEAIGGERDRVDKVWFVDDSADPRDATLRAVGAVAGGA
                     PQEIAKPSVLRVDRRALGKWLEPARGHALEDHLYVVDPRGNWMMRAPANADPGRLKRD
                     IDRLLRASASWDRPSR"
     gene            complement(10313..>10889)
                     /locus_tag="VFF72_05260"
     CDS             complement(10313..>10889)
                     /locus_tag="VFF72_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZV92603.1"
                     /translation="ARALAREPAAVAAQFDRRVRLAGQWINDKTVFLDNRQMQGRVGF
                     FVVTPLRLDRSPDVVLVERGWVARNFVDRNSLPAVVTPAGEVEVDGRVAATPSRLFQF
                     ADAASGVIRQNIDPRAYARETGLPLLPLSIVETPTAANAHDGLTRIWSAPAVDVQMNY
                     GYAFQWFAIGAVIVFLYVRFALLRPRRRRDD"
BASE COUNT         1847 a         3777 c         3641 g         1624 t
ORIGIN      
        1 cgctatccgg gcgtccgtgc cgattccgac atgtacaccc tggcctactc gtttcgcccc
       61 tggcgccagc ccagggcgat cgtcgacggc gcgacgatcc tctcgtacct gcaggacacg
      121 gcgcgcgaga acggcatcga ggagcgcatc cgctacgggc atcgcgtcac cggcgcggcg
      181 tggtcgacgg cagatgcgcg gtggacggtc gaggcgcgat gcgccgaaag cgaccaggtc
      241 gtccgcttca gctgcaattt cctcttcgcg tgcagcggct actaccgcta cgccgccggt
      301 cacgcgccgg agatcgccgg ccgcgatcat taccgtggcc gcgtcgtcca tccccagcac
      361 tggagcgacg acatcgacca cgcgggcagg cgtgtcgcgg tgatcggcag cggcgcgacg
      421 gcgatgtcgc tggtgccggc catcgcggcg acggcttcgc atgtcacgat ggtgcagcga
      481 tcgccgacgt gggtcatctc gcaaccggcc gacgaccgcc tggcacgccg cctggcgcgg
      541 ttcctgcccg atcggctcgc ctacaccgcc gttcgttggc agcgggtgct gatgggcgtc
      601 ttcttcttcg ccttgtgcag agggcgcccg gcactcgcga gtcgcctcct gacggccgga
      661 gtccgagccc gggtcgggcc gcagttcgac atcgccaccc atttcgcgcc gcgctacaag
      721 ccctgggagc agcgcttgtg cctggttccc gacggcgact tcttccggac ccttcgcagc
      781 ggccgggcca gcatcgtcac gagcgagatc gaacgcttca cggagaccgg cctgcgtctg
      841 cgatcgggcg acctcgtcga tgccgatctg gtcgtgatgg cgaccggcct cgagctcgag
      901 ttgctcggcg gcattgcagt gagcgtcgac ggccgcgtcg tcgactgggc gtcgaagctc
      961 acctacaagg gcatgatgta cgacggcgtg cccaatttcg cgtccgtctt cggttacgcg
     1021 aacgcgtcct ggaccttgcg atccgacctg atctccggat acgtctgccg gttgctccag
     1081 cgcatgaacc ggcggggcct gcgtcagtgc acgccacgaa acagcgaccc gggcatcacc
     1141 acggcgccgt ggctggacct gtcttccgga tacatccggc gcgccgccga gcgcttgccc
     1201 aagcagggct cgcgggcgcc ctggagcctg tgccagaact atcttcgcga cctggcgcca
     1261 atgcgttggt ctcggctcga cgacggcgcg atggtcttct cgaatcccgg cggccgcccg
     1321 cccgtcgcgt ctgcgccggg ctgcggcaag gcgccgacta aaatcgtcgg ctcaccgctg
     1381 cgagaccgtt ctccatgagc ctgaaatgcg gcatcgtggg cctgcccaac gtcggcaagt
     1441 ccaccctctt caacgcgctc acgaaagccg ggatcgccgc ggagaactat ccgttctgca
     1501 cgatcgagcc caacgtcggc atcgtcgagt tgcccgaccc gcgcctcgcc cgcctcgccg
     1561 aaatcgtcaa gcccgagcgc atcgtcccgg cgatcgtcga gttcgtcgac atcgccggcc
     1621 tcgtcgccgg ggcatcgaaa ggcgagggac tcggcaacca gttcctcgcc catgtccgcg
     1681 aaaccgatgc gatcgtcaac gtcgttcgct gcttcgacga cgagaacgtg atccacgtcg
     1741 ccggcagggt cgacccggtc gccgatatcg aggtgatcca gaccgagctc tgtctcgccg
     1801 acctggcgac cgtggaaaag agcctcgccc gccagctgaa ggcggccaag tcgggtaacg
     1861 acaaggaagc caagcgcctc gtcgacgtgc tcgagaagtg caacgcgacg ctcgaccagg
     1921 cgcggccggt gcgctcgatc gacttcagca aggaagagct cgcggtcctc aagccgcttt
     1981 gcctcatcac cgccaagccg gcgatgttcg tggccaacgt cgccgagaac ggcttcgccg
     2041 acaacccgca cctcgaccgc ctgcgcgagg tcgccagccg ccagcacgct cctgtcgtcg
     2101 cgatttcggc gaagacggaa gccgagctcg ccgacatgga cgatgccgac cggcagatgt
     2161 tcctcgccga gatggggcag accgagtcgg gcctggcccg cctggtccgc gccgccttca
     2221 cgctcctcgg gctgcagacc tacttcaccg ccggtccgaa ggaagtccgt gcctggacga
     2281 tccacgccgg cgacacggcg ccgcaggccg ccggcgtcat ccacaccgat ttcgagcgcg
     2341 gcttcatccg tgcccagacc gtcgcctacg aagacttcat ggcctacggc ggcgaacacg
     2401 gcgcccggga cgccgggcgg atgcgcgccg aaggcaagga ctacgtcgtc aaggacggcg
     2461 acgtgctgaa cttcctgttc aacgtctagg acaggccccg cctgccacgc tgcgccgacc
     2521 ggtccatgac acagacggcc gaaccgccgc cgcgccatac acccgcgtcg agctggcagc
     2581 tcgagttgcc ggcgttcgcg gcgaccgtgg tcgcgtacgt cgcggcgggt ctcgtcgcgc
     2641 tgctcctgac gatcgcgccc gcaggcacct cgccgttgta tcccgcagcc ggcatcgccc
     2701 tggccagcgt cctcgtccag ggctggcgga tgctcggcgc tgtcgccctc ggctccttga
     2761 gcgtcaacct catcagcctg tcgctgcacg gcgccgggga tcatcgcgtt gccacggccg
     2821 cggtcgtggc cacgggcgcc gtgctgcagg cggcactcgg cgcagcgctg atcagacgct
     2881 acgttcgtca acctctggtc ctgaccttgc cggccgacgt cgcgcgcttc atggccgcgt
     2941 gcctcggagc cagcctggtg gcgccgacga tcggcgcact cgcgctgagc ctgttttccg
     3001 cgttgccgcc gggaggcgcc gtgtggcact ggacgacgtc gtggatcggc gatctcgccg
     3061 ggctgctgat cgtcactccc atcgcgctga cgatcttcgg caggcccgca tcggagtggc
     3121 ggccgcgacg agtctccgtc ggcgtgacgc tttcggtggt ggtcgtcttt ctggcgctcg
     3181 gcatcgtcca gctcggccgc tggaacgacg agcgcgttcg cgaagccttc gagcacgacg
     3241 cttcgagcgc ttcgctgatg ctggcgacgc agatgcagga gccccttcga gctctcgagg
     3301 ctctgcacga tgtcttcacc gtctcgcacc agatcggcgg cgccgaagtt cgccgcatca
     3361 gccagcactg gctgaacgga acctccgtcc gcgccatcgg ctggagcgag cgggttcatc
     3421 acgacgacat cgccgccttc gaagcgcgcg cccgcgccga cggggcactc ggctaccgca
     3481 tctacgatcg accggcggtg cctggcgcgc gcccggtggc gcctgtggcc agcctcgaat
     3541 ccgacctcgg gctggggctt cgcggcgatg gcggcgacgt gatcgccatt cgcttcgtcg
     3601 agccgtcgga gatgaacggc gccgcactcg gcctcaacgc cgtctcgatt ccgaccggcg
     3661 gcgctgccgc cctgatcgcc gcggtcgact cgggcgcgcc cgcggtgtcg accggctttc
     3721 aaatgacgcc agaaggcgac ggcgaggcgc agccaggggt ccttctctac caggccgtct
     3781 acgacggcga tccggttcct ccggggcaac gtcgctcggc atttcgcggc ctcgttttcg
     3841 tcgtttcctc gctcgatcgt cagctcgcct cgatggccgg cagggtcccg gcatacctcg
     3901 acctctgcgt cgtcgacgca acacccggcg ggtcgccccg ccgtctcggc ggccggcccg
     3961 ggtgcgacac cggcacgccc gagcttctcc acgatcgccc gatcgtcttt gccgggcatc
     4021 cgtgggattt gcgcatctcg gcccgtcccg acgccgtccc cgatgcgaac gatcgcagcg
     4081 cctggatctt ctcggccgcg gggttgcttt ccgctgctct gctcggcgct tcgctcctca
     4141 tcacgaccgg gcgcacccga cgcatcgaaa gcgccgtccg cgaccggacg gccgcgctgc
     4201 gcgccgaggt cggcgagcgc catgcggccg aggcggctct gcgggcgagc gaggagcgat
     4261 tccgcaacat cctcgaccac gtgccgatcg gcatcgtcta cgccgggctc gatggccgaa
     4321 tgatccaggc caatccgcgc ttttgcgagt tgacgggata cagcgaggcg gagctcgtcg
     4381 ccctctcgcc ggccgacctg accgaccccg acgacctggc ccacgaccag gcgctgaccg
     4441 ccgagctcgt cagcgaaaag attccgatgt accgccggca tcgacgctac atcacgaaaa
     4501 agggcgacgt ggtctgggtg cgcgcgaccg tcacgctgct gcgcgattcg gcgcagcagc
     4561 cctggcgcat cgtcggcgtc gtcgaagaca tcaccgagca tctccggctc gaggaggccg
     4621 agcgcgcccg ggaggccgct gaggccgcga acgtcgccaa gagcgagttc ctgtcgcgaa
     4681 tgagccacga gcttcgaacc ccgttgaacg cgatgctcgg tttcgcacag ctgctcgaga
     4741 tcgacaagcg gcagcctctt tccccggtgc agcggccgtg ggtcgcgcag atccagcagg
     4801 ccggatggca cctgctcgag atgatcaacg acgtgctcga cctgtcgcga atcgagtcgg
     4861 gcaacctgcg cctgcagatc acggccctcg agctgccgga gctggtggcg gcgacggtgg
     4921 cgctcgtcgg cagcgaagcc gtccgccgga acgtgcgcat ctcgcaagat ctcggcccgg
     4981 gcacggccgg catcctcggc gatccgacgc gggtcaagca gatcctgacg aacctcctca
     5041 gcaacgccgt caagtacaac gtcgatggcg gccgggtgca tgtcgccagc cgcctgacgg
     5101 ccgcggaccg ggtcgagatc tcggtcaccg acacgggcat gggcatgacc gccgagcaga
     5161 tgtccgggtt gttcaagccg ttcaatcgcc tcggccgcga acgctcggcg ctgcagggaa
     5221 ccggcatcgg gctggtcatc agccggcggc tcgccgagct gatgggcggc tccatcaccg
     5281 tgcgcagcct gccgggcgag ggatcgtcgt tcactctcag gctgccgaag tcgatcgatc
     5341 ccgataccgt cccttcgccg ctggacgccc tcgagaccgg gccggccgaa taccaccggc
     5401 gaatcgttca ctacatcgag gacaacgaga ccaacgtcga ggtcatgcgc ggcatcctct
     5461 cgcagcggcc ccaggtgcag atggaggtgt cggtgaccgg tctcgatggc ctggccgcgg
     5521 ttcgagcccg caggccgcac ctgatcctgc tcgacatgca cttgcccgac atcaacggcc
     5581 tcgaactgct gcgccacctg aagtccgatc ccggaacggc gggcgtgccg gtgatgatcg
     5641 tctcggccga tgcgctcgcg ccgcagatcg atgccgcgct cgccgcaggc gccagccgct
     5701 acctgaccaa gcccgtcgac gtcgccgagc ttctcggcgc gctggacgac cttctcgacc
     5761 ggatggacac cggattcagc tagagcaagg cgggcacccg ccttgccccg ccgataggga
     5821 ggcccgatcg aggcgattgc cgctgccttg gcactctcga gcctcgagtg ctaatatttg
     5881 cggaacgaaa gggtgatcgg cttgtctgat atcactatga accattcccc tgctgccact
     5941 gctctgaccg tccgcgaccc ttgggccctc gtgccgtcgc tgggcaatct ggacgcgtac
     6001 atctcggcga tcaaccggat tcctcttctg accgccgaag aggagtcgag gttcgcccgc
     6061 gagttgcgcg ccaccggcga cctgggagcc gccggaaagc tcgttctctc gcaccttcgc
     6121 ctggtcgtgt cgatctcgcg caagtatctc ggctacggtc tgccgcatgg cgacctcatc
     6181 caggaaggca acatcggcct gatgaaggcg gtgaagcgct ttgatccgga ccagggcgtc
     6241 cggctcgtga gctacgccat gcactggatc aaggccgaga tccacgaata catcctgaag
     6301 aactggcgca tggtcaaggt cgcgacgacc aaggcgcagc gcaagctctt cttcaacctg
     6361 cgctccatga agcagagcct caaggacgac gcggtggcgg gcgacacgca tcgttcgacc
     6421 ttgacgcaga gcgaggtcga catgcttgcc ggcacgctca atgtcaagcg cgaggaagtt
     6481 ctcgagatgg agacgcgcct gtcgggcggc gacgtcgcgc tcgagccgca gaacgacgac
     6541 ggtgaagaaa gctacgcgcc gatcgcctat cttgccgacg acaacagcga gccgacgcat
     6601 gttctcgagt ccaggaaacg cgactggctg gctgccgacg gcatctccct ggcgctggag
     6661 tcgctcgacc cgcgcagccg gcgcatcgtc gaggaacgct ggctcaaggt gaacgacgac
     6721 agctccggtg gcatgactct gcacgacttg gccggggaat acggcgtcag cgccgaacgc
     6781 atccggcaga tcgaggtggc ggccatgaag aagatgaaga aggctctcgc gcacgtcacc
     6841 tgacgaacag gtcgagcctt ctcacaagcg gctccctcgg gggccgcttc gcttttcggg
     6901 gcagcctgcc gaccgtgcga tctcaggcag ccgagagaag ttggctcaga tcctgctcga
     6961 aggccttctg atcggcaccg tagggcacgt acaggcgcac cttgccctgg gtgtcgaaga
     7021 cgaaggaggc tgcgctgtga tccatcgtgt agctgccggg cgtcttgccg ggcaccttcg
     7081 cgtagaagac cttgaattcc ttcgccgcct cggccgtctg ctcaggcgaa ccgcgcagtc
     7141 cgaccacgtc cggtccgaag ttggagacgt agcttttcat cacctgcggc gtgtcgcgct
     7201 cgggatcgac ggtgacgaac aagacttcca cgcgccggcc cgccgccccg aggtccttct
     7261 tgacctgagc cagctccgtc agggtcgtcg ggcagaagtc gggacactgc gtatagccga
     7321 aaaagaccac gatgaccttg cccttccagt cggcgaggct tcgggtgcgg ccgtcggcat
     7381 cgggcagctc gagacgccgg gcgaactcgg caccggtgat gtcggtgaaa tggaacctgg
     7441 gctcgctcga acgacccaga agacggtcgc accccggcag cggcaggatc gcaaggccgg
     7501 ctgccccgat caggcatcgt cgagaaagag tcatgccagc atcggccaga ggtagtgatc
     7561 gaccatgagt gctgcgaaca gcagcgaaag atggacgatc gagaagcgaa acgtccgccg
     7621 tgccagcgcg tccgaataat gtcgccagag ccgccatgcg taaccgcaga acatgacgcc
     7681 gaggacgatc gcggcggcca ggtagatcgg cccgctcatg ccggccacga aaggcagcag
     7741 cgtcgccgcg aacagcacga tcgtgtagag gaagacgtgc agccgggtga actcggaacc
     7801 gtgcgtcagc ggcagcatcg gcaagccggc ccgccggtaa tcctcggcgc ggtacaacgc
     7861 gagtgcccag aagtgaggcg gcgtccagag gaagatgatg aggcacagga tgagcgcttc
     7921 ggcgccgacg tcgccgcgga tggccgccca gccgagcacc ggcggcatcg cccccgaagc
     7981 gccgccgatg acgatgttct gcggcgtcat cggcttcaac agcaccgtgt agacgaccgc
     8041 gtagccgacg aaggtcgcga aggtcagcca catcgtcaac ggattgacga aaagccagag
     8101 aagcgtgctg ccgccgatgc acagcgccac ggaaaacgca agcgcctggc gggtggtcag
     8161 ctcgccggtg gcggtcggcc gccacgaggt ccgcgccatg cgcgaatcga tgcgttgctc
     8221 gaccaggcag ttgaatgccg ccgcggctgc cgcgaccagc cagatgccga tcaccgccgg
     8281 cacggccttg cgccaagcgg gaagatcggg agtcgccagg aacatgccga tcgctgcaca
     8341 gaagacgatc agctggacca ctcgcggctt cgtcagcgca tagaactggc gcgcgcggga
     8401 cggcagggag gcgcgctgcg gcgcggcgga cgcgagggta tcggacatga cggacaacgc
     8461 ccgcattcta aggagcgacg tgctgcgcac ggctcggcca aggcgattcg acacgttggc
     8521 gcaagggccg ggaacgcgcg agaagcgtcg tcagcatgac gacgagcgcc gcgctgcctg
     8581 cggtgtgcgc gagcgcggtc aagagcggcc agccaagcag gacattgccg aggccgctcg
     8641 ccagttgcca ggcggcgatc cccgccagcc acccgcccca tcgacgcgcc tgcggatcgc
     8701 ccgccgcagc caggcgcgcc gccagccaca gcagcatcgg caggaccacg agcgcgccga
     8761 ttcgatgcgc catgtggatc gcggtgaggg cctggaaagg caggaagccg ccctcgccgc
     8821 tgatgccgag cggcctcagc acgacaaagc cttcggcgaa attcatgtcg ggccaccact
     8881 ggccctggca ggtcgggaaa tcccggcagg cgagcaccgc atagttcgtg ctcacccagc
     8941 cgccgaggat gatctgcacc aatgccagtg ccgccacgac cgccaggccg acgcgcaacg
     9001 tccggccgag cggcaatggc cgcggctcgt acagctctgc ctgcagggcc agcatcgcga
     9061 gcaacccgag gccgccgacg agatgaagga cgacgatggc cggatagagg ctcatcgtca
     9121 ccgtcaacgc gccgaaggcg ccctgcgcga tgatccagag cacggtggca accgcccagc
     9181 gaggcgacgc gacgagccgg ccgcgccgtc gctcgaccac cgccaggatc gccagggcga
     9241 tcacgaggac gccgatcgcc gtcgccgaat accggtgggc catctcgatc caggccttcg
     9301 tgtgggtcac tgcaccggtc ggcacggcgg cctcggcggc gttgatcggc gcgcgcgctc
     9361 cgaccggact cgcgtgcccg tagcagcccg gccaatcggg acagccgagc ccggaatcgg
     9421 aaaggcgggt gaaggcaccg aggatcacga ggtcgaagct gaggaacagc gccagcaacg
     9481 ccagggcacg cagccgcgcg ccggtggcgt cgccgcgtcg ccggcgccag aaccagatga
     9541 gcggccccga ggcgagaacc aggcccgtcg caagaatcgc gaagacgggc gccaggtcgt
     9601 acgccggaac ggcggcactc atcggctcgg tcggtcccaa ctcgcggacg cgcgcagcag
     9661 gcgatcgata tcgcgcttca ggcgccccgg gtcggcattc gcgggggcgc gcatcatcca
     9721 gttgccgcgc ggatcgacga catagagatg atcctcgagc gcgtggccac gggccggctc
     9781 gagccatttc ccgagggctc gcctgtcgac tcgcagcacc gacggcttgg cgatttcctg
     9841 cggcgccccg ccagcgaccg cgccgacggc acgcagcgtc gcgtcgcgag gatcggccga
     9901 gtcgtcgacg aaccagacct tgtcgacccg gtcgcgctcg ccgccgatgg cttcgtgaag
     9961 ctgacgctgc aaccacagcc ggtgctcgca gttcgcgtcg caggcgcctc ccgaaacgac
    10021 gacgagcagc cactggccct gcagcgccgg cgcagcgacg gcgcggccgc gaagatcgcg
    10081 caatggcagg tcggccggca acggcctcgg cggcacgatc agatcgctgt agttcgtgag
    10141 cgtgcttggc cggaggtcga agtaaaaggc gaggtacgaa gccaccacgg gggcggcgca
    10201 gatcagaagc acgacgatca tcgtccagcg cccgcgacgg gtccgcgaag cggcgtcggg
    10261 gccggcctgg ggatcgggca acgaatgaac gctcaggttc agcaagggag ggtcagtcgt
    10321 cacggcggcg tcggggacgg aggagcgcga agcggacata gaggaagacg atgaccgcgc
    10381 cgatggcgaa ccactggaag gcgtagccgt agttcatctg gacgtcgacg gccggcgcgg
    10441 accagatgcg cgtcaagccg tcgtgggcgt tggccgctgt cggggtctcg acgatcgaca
    10501 gcggcaacag cggcaggccg gtctcccggg cgtacgcccg agggtcgata ttctgacgga
    10561 tgaccccgct ggcggcgtcc gcgaactgga acagccgcga cggcgttgcg gcgactcgac
    10621 cgtccacctc gacttctccc gccggcgtga cgactgccgg aagcgaattc cggtcgacga
    10681 agttgcgtgc cacccagccg cgctcgacga gtacgacgtc gggcgagcga tcgagccgaa
    10741 gcggcgtgac gacgaaaaag ccgacgcgtc cctgcatctg ccggttgtcg aggaagacgg
    10801 tcttgtcgtt gatccactgt ccagccagac gcacccggcg atcgaactgc gcggcgacgg
    10861 cagccggctc gcgggcaagc gcacgcgcc
//
LOCUS       DASRWS010000284         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1561862, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000284 DASRWS010000000
VERSION     DASRWS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561862"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFF72_05265"
     CDS             complement(<1..214)
                     /locus_tag="VFF72_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZV92604.1"
                     /translation="MDAPRFATLVVGHDARNRRIARLRLNRPERLNAIDPRMPAEIRA
                     AVDWANADDEVHVVVVEGEGRAFCAGY"
     gene            complement(237..908)
                     /locus_tag="VFF72_05270"
     CDS             complement(237..908)
                     /locus_tag="VFF72_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92605.1"
                     /translation="MRRSRLALSLAVAAVAGVLAACGSMNMARPADMTFFVSSVGSGK
                     GGDLGGLAGADRLCTSLATAAGAGGHTWHAYLSTVPHDGMKGVNARDRIGTGPWRNAR
                     GVVIAQDVVDLHGPTNNLNKQTALTEKGEVVNGYGDTPNEHDILTGSQPDGTAIAGGI
                     DTTCHNWTSSGEGAAMLGHSDRTGPNDSAPLKSWNSAHQSRGCSVDALKSTGGDGRLY
                     CFATN"
     gene            1078..2151
                     /locus_tag="VFF72_05275"
     CDS             1078..2151
                     /locus_tag="VFF72_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HZV92606.1"
                     /translation="MPSRRSILAAALLAPHLSHAAPGLPAQPGLVFPRDFGSHPETRI
                     EWWYVTGSLEAAARRWGFQVTFFRAATGFADSASRFAAGDVLFAHAAITDLEPARIRF
                     DQRIARPGFGIAEARPGDTSLRLRDWRLGRESASGPSRYHASVASERAAFAFELTFDA
                     TEPVLVQGQDGISRKGPAPAEFSHYYSEPQLAARGHVALDGRAHEVTGRAWLDHEWSD
                     SFLAPDAVGWDWIGMNLDDGAALTAFQIRRADGTTLWAGGSHRPRGGPTRNFAPHEVT
                     FEAERRWTSPATRAIYPVRSRIVTPAGRFVVAALLDDQEIDSRASSGAVYWEGLSDLL
                     VAQGRRVGGGYLEMTGYVAPLVL"
     gene            complement(2168..>3011)
                     /locus_tag="VFF72_05280"
     CDS             complement(2168..>3011)
                     /locus_tag="VFF72_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92607.1"
                     /translation="TQIIVLASVAAIGFALFLAWELTEEHPVVDLRLFGRRNFLWGTL
                     ALSVAYGLFFGNIVLLPLWLQQYLGYTATWAGMATAPVGILAIMLSPWVGKNVSKIDP
                     RKLATVAFLGFGLIMWMRSRFNVQADFASILVPTFLQGAAMAFFFIPLQAIIFSGLAP
                     NRIADAAGLSNFVRITAGAIGTSIFTTAWENRAILHHSEIAETVNRGNDAATATIAQL
                     GQGGLDTSQALAMINRLIDQQSYTMAATDLFYISSVLFVFLLVFVWMTKPTLGAAAAA
                     GGAH"
BASE COUNT          463 a         1041 c         1057 g          450 t
ORIGIN      
        1 cgtaaccggc acagaaggcg cggccttcgc cctcgacgac gacgacgtgc acctcgtcgt
       61 cggcattggc ccagtcgacg gcggcacgaa tctcggccgg catccgcgga tcgatggcgt
      121 tgagccgctc cggccggttc agacgcagcc gggcgatgcg ccggttgcgg gcgtcgtggc
      181 cgacgaccag cgtggcgaag cgaggcgcat ccatcggatc gttcgccggc gccggttcag
      241 ttcgtcgcga agcagtagag ccggccgtcg ccgccggtcg acttcagcgc atcgacgctg
      301 cagccgcgcg actggtgcgc cgaattccac gacttgagcg gcgcgctgtc gttcgggccg
      361 gtgcggtcgc tgtggccgag catcgccgcg ccttcgccgc tgcttgtcca gttgtggcag
      421 gtggtatcga tgccgccggc gatcgcggtt ccgtcgggct gcgagccggt aaggatgtcg
      481 tgctcgttcg gcgtgtcgcc gtagccgttg accacctcgc ccttctcggt cagggcggtc
      541 tgcttgttca ggttgttggt gggaccgtgc aggtcgacca cgtcctgggc gatgacgacg
      601 cccctggcgt tgcgccacgg tcccgtgccg atccggtcgc gtgcgttaac gcctttcatg
      661 ccgtcgtggg gcacggtgct gagatacgcg tgccaggtgt gacctccggc gccggcggcc
      721 gtggcgagcg acgtgcagag ccggtcggcc ccggcgagcc cgccgaggtc gccgcccttg
      781 cccgagccga cgctcgaaac gaagaaggtc atgtcggccg gtcgcgccat gttcatcgag
      841 ccgcaggcgg cgaggacgcc cgccacggca gcaacggcaa gagagagggc aagcctcgat
      901 cgacgcatga acggctcctg aagatggatg gatgaagact ggcttgtcac ggcgacgaag
      961 cgcgctgacg cggcgcagac tagtgcaaag cgctcgccgc ttcaagccgc ggtcatcggc
     1021 gcaccggggc atccggccgc gccgcgacgc acatcgaaag ccgtgtcatg ctagacggtg
     1081 ccctcgcgtc gatcgatcct ggccgcggcc ctcctcgcgc cgcacctgtc gcacgccgcg
     1141 ccgggcctgc ctgcgcagcc cggtctcgtc ttcccgcgcg acttcggctc gcatcccgag
     1201 acccggatcg aatggtggta cgtcaccggc agtctcgaag ccgcggcgcg gcgctggggc
     1261 ttccaggtga cgttctttcg cgccgcgacg gggttcgccg actccgcgag ccgattcgcg
     1321 gccggcgacg tgctcttcgc gcacgcggcg atcaccgatc tcgaaccggc gcggatccgc
     1381 ttcgaccagc gcatcgcccg cccgggcttc ggcatcgccg aggcccggcc gggcgatacg
     1441 tcgctgcgac tgcgcgactg gcggctcggg cgcgaaagcg cgtcgggccc gagtcgctat
     1501 cacgcctcgg tggcgagcga gcgggccgcc ttcgccttcg agctgacgtt cgacgcgacc
     1561 gagcccgtcc tcgtgcaggg gcaggacggc atctcgcgca agggtcccgc gccggccgag
     1621 ttcagccact actacagcga accgcagctc gccgcgcgcg gccacgtcgc cctcgacggc
     1681 cgcgcccacg aagtgaccgg ccgagcctgg ctcgatcacg agtggagcga ttccttcctc
     1741 gcgcccgacg cggtcggctg ggactggatc ggcatgaacc tcgacgacgg cgccgcgctg
     1801 acggccttcc aaatccgccg cgccgacggc acgacgctgt gggccggcgg cagccatcgc
     1861 ccgcgcggtg ggccgacgcg caacttcgcg ccgcacgagg tcacgttcga ggccgaacgc
     1921 cgctggacga gcccggcgac gcgggcgatc tacccggtgc gctcgcgcat cgtcacgccg
     1981 gccggacgct tcgtcgtcgc cgccttgctc gacgaccagg agatcgacag ccgcgccagc
     2041 agcggcgcgg tgtactggga aggcctgtcg gaccttctcg tcgcgcaggg ccgccgcgtc
     2101 ggcggcggct acctcgagat gaccggctac gtcgcgccgc tcgttctctg agcgtgccgc
     2161 ggcgcgatca gtgcgcgccg ccggcggcag ccgctgcgcc gagcgtgggc ttggtcatcc
     2221 agacgaagac gagcaggaag acgaacagca ccgacgagat gtagaagaga tcggtcgccg
     2281 ccatcgtgta cgactgctgg tcgatgagcc ggttgatcat cgcgagcgcc tgcgaggtgt
     2341 cgaggccgcc ctgtccgagc tgcgcgatcg tcgccgtggc cgcgtcgttg ccgcgattga
     2401 cggtctcggc gatctcggaa tggtggagga tggctcggtt ctcccacgcc gtcgtgaaga
     2461 tcgaggtgcc gatcgcgccg gcggtgatgc gaacgaagtt gctcaggccc gcggcgtcgg
     2521 cgatgcggtt cggagccagc cccgagaaga tgatcgcctg cagcgggatg aagaagaagg
     2581 ccatcgccgc gccctgcagg aacgtcggca ccagaatcga cgcgaaatcg gcctggacgt
     2641 tgaagcgcga acgcatccac atgatcagcc cgaagccgag gaaggccacc gtcgccagct
     2701 tgcgcggatc gatcttcgag acgttcttgc cgacccatgg cgacagcatg atcgccagga
     2761 tgccgaccgg cgccgtcgcc attccggccc acgtcgccgt gtagcccaag tactgctgaa
     2821 gccagagcgg aagcagcacg atgttgccga aaaagaggcc gtaggcgacc gacagcgcca
     2881 gcgttcccca aaggaaattt cgtcgcccga aaaggcgaag gtcgaccacc ggatgttcct
     2941 cggtgagctc ccaggcgaga aagagcgcga agccgatcgc cgccaccgac gcgagcacga
     3001 tgatctgggt c
//
LOCUS       DASRWS010000285         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1561896, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000285 DASRWS010000000
VERSION     DASRWS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561896"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFF72_05285"
     CDS             <1..841
                     /locus_tag="VFF72_05285"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZV92608.1"
                     /translation="GGGYGSDAAWLRDELARVRAATTRPWGVGFITWSTDPGILEVAL
                     AHEPHAVMLSFGDPRPFGRVVRAAGCKLICQVQDLAGARLAAEAGADLVVAQGSEGGG
                     HGGTRATLPLVPAVIDAVAPIPVIAAGGIADGRGLAAALLLGAQGALIGTRFYASDEA
                     LGHAAAKAAIVSGSGDQTTRTTIFDTVRDLAWPLPYTGRALRNDFMARWHGREKELAS
                     ALSTERGRYRQAASTGDVDTAVIWAGEGIDLIDALGAAGETMRRMADDAEARLRDVPR
                     LLS"
     gene            complement(949..1350)
                     /locus_tag="VFF72_05290"
     CDS             complement(949..1350)
                     /locus_tag="VFF72_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZV92609.1"
                     /translation="MTARRLFVAGVLSTCLLAPASAAPDAARGEQVYARCEACHALAY
                     DRVGPRHCGLIGRVAGSVPGFAYSAAMKNSHIVWNGKTLDRFLASPLKMVPGTAMTYD
                     GVPDARDRADLIAYLESVDRLPQCKALKAKR"
     gene            complement(1347..1919)
                     /locus_tag="VFF72_05295"
     CDS             complement(1347..1919)
                     /locus_tag="VFF72_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZV92610.1"
                     /translation="MSFLQMPTPVVARRMFLGRSTGLVLSGAAVALLAGNDALAASSG
                     GGDPKDVQILNTALGAELEAIAAYQLGAESKLLQKPVLDLAVTFQGHHKEHAELLSKT
                     IVKLGGHPVAAKADYHFPAEQLKSQADVLRFAAKLEQGAVSAYLGAVPLFANRDLSKA
                     AASILGDEAMHWAVLRNALGEAPVPVAFMS"
     gene            complement(1949..>2324)
                     /locus_tag="VFF72_05300"
     CDS             complement(1949..>2324)
                     /locus_tag="VFF72_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZV92611.1"
                     /translation="VDEQADDPGMEDDVDRGPERVAMRGELRRLMEKRIDTLPDVFRT
                     VFMLRAVEELSVEETAAALEIPEATVRTRFFRARSLLREGLSRDVDFAIADAFSFAGA
                     RCDRIVASVLASLSDDREDSRP"
BASE COUNT          347 a          822 c          816 g          339 t
ORIGIN      
        1 cggcggcggt tacggcagcg acgcggcctg gctgcgcgac gaactcgcgc gcgtgcgcgc
       61 cgccaccaca cgcccctggg gcgtgggctt catcacctgg tcgaccgatc cgggcatcct
      121 cgaggtcgcg ctggcccacg agccgcatgc ggtgatgctg tcgttcggcg atccccggcc
      181 attcggacgc gtcgtcaggg cggccggctg caagctgatc tgccaggtgc aggacctcgc
      241 cggcgcgcgg ctcgccgccg aagccggtgc agacctcgtc gtcgcccagg gcagcgaagg
      301 cggcggccat ggcggaacgc gggcgaccct gccgctggtg cccgccgtga tcgacgccgt
      361 cgcaccaatc ccggtgatcg ccgctggcgg catcgccgac ggacgcggcc tggccgcggc
      421 gctcctgctc ggcgcgcagg gcgccctgat cggcacgcgc ttctacgcca gcgacgaggc
      481 gctcggccac gcggccgcca aggcggcgat cgtctcgggc agtggcgacc agacgacccg
      541 caccacgatc ttcgacacgg tgcgcgacct cgcctggccc ctgccctaca ccggccgcgc
      601 gctgcgcaac gacttcatgg cacgctggca cggccgcgag aaggaactcg cgagcgcgct
      661 gtcgacggag cgcggccgct accggcaggc ggcaagcacg ggcgacgtcg acaccgccgt
      721 catctgggcc ggagaaggca tcgacctcat cgacgcgctc ggcgccgccg gcgagacgat
      781 gcgccgcatg gccgacgacg ccgaagcacg gctgcgcgac gttccgcgcc tgttgtcatg
      841 accgtcgcgc tccggcgcgg cccgcagtcc cggccttcgc ctttcgaggc cttgtaccgg
      901 catctgtttg acttggcggc tggctgctcg cgccgctccg gcgcgagctc agcgcttcgc
      961 cttcagggcc ttgcactgcg gcaaccgatc gacgctctcc aggtaggcga tcaggtcggc
     1021 gcgatccctg gcatcgggaa cgccgtcata cgtcatcgcc gtgccaggaa ccatcttcag
     1081 cggcgaggcg agaaaccggt cgagcgtctt cccgttccag acgatatgcg agttcttcat
     1141 cgctgcggaa taggcgaacc ccggcacgct ccccgcgacg cggccgatga gaccgcagtg
     1201 gcgcggaccg acgcggtcgt aggcgagcgc atgacaggcc tcgcagcgcg cgtagacctg
     1261 ctcgccgcgc gcggcgtcgg gcgccgccga cgccggcgcc aaaaggcagg tcgagagcac
     1321 gccggccacg aagagccggc gcgcggtcat gacatgaacg cgacggggac cggcgcttcg
     1381 ccgagcgcgt tgcgcaacac cgcccagtgc atcgcctcgt cgccgaggat gctggccgcc
     1441 gctttcgaga ggtcgcgatt ggcgaacagc ggcaccgcac cgaggtaggc gctgaccgct
     1501 ccctgttcaa gcttggccgc gaatcgcagc acatcggcct gcgacttcag ctgttcggcc
     1561 gggaagtgat agtcggcctt cgccgcgacc ggatggcctc ccagcttcac gatggtcttg
     1621 ctcagcaact cggcgtgctc cttgtggtgc ccctggaagg tgacggccag gtcgagcacc
     1681 ggcttttgca gcagcttgct ctcggccccg agctgatacg cggcgatcgc ttcgagctcg
     1741 gcgccgagcg cggtattgag gatttgcacg tccttcggat cgccgccgcc tgacgacgct
     1801 gccagcgcgt cgttgccggc gaggagcgcg acggccgcgc ccgaaaggac gaggcccgtg
     1861 gaccggccga gaaacatccg gcgcgccacc accggtgtcg gcatttgcag gaaggacatg
     1921 gcgatctcct cgaatcgttg agttgttgtc agggccggga atcttcccgg tcgtcggaaa
     1981 ggctggcgag cacgctggcg acgatgcggt cgcagcgcgc gccggcgaac gagaaagcgt
     2041 cggcgatggc gaaatcgacg tcgcgcgaca gaccttcgcg caacaggctg cgggcgcgaa
     2101 agaagcgggt gcgcacggtg gcttcgggga tctcgagcgc cgcggcggtt tcctccacgc
     2161 tcagctcctc gacggcgcgc agcatgaaga cggtgcgaaa gacatcgggc agcgtgtcga
     2221 tgcgcttttc catgagccgg cgcaattcgc cgcgcatggc cacgcgctcg gggccgcggt
     2281 cgacgtcgtc ttccatgccc gggtcgtcgg cttgctcgtc gaca
//
LOCUS       DASRWS010000286         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_116410, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000286 DASRWS010000000
VERSION     DASRWS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116410"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFF72_05305"
     CDS             complement(<1..1056)
                     /locus_tag="VFF72_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZV92612.1"
                     /translation="MADALRPYLRKRDFSITREPRGGKRSKSGQLAFVVQKHAASHLH
                     YDFRLELGGTLKSWAVPKGPSLDPTVKRMAVHVEDHPMAYAGFEGDIPKGQYGGGTVI
                     VWDNGTWEPVGDAQAGYAAGKLKFRLHGHKLSGGWTLVRMHGRATERQDPWLLIKERD
                     ESARPASQYSVVDAEPASVLSDRTIPDPAPTATRKSTSKSGAATGATSGPSTATATTK
                     ATTKATTATATRPATRTRTSTPKTPRSSTAGTASKPVAAGARAPARPSRPAHPAVPDG
                     AKKAALPATLSPQLATLVAQAPDDDGWTYEIKFDGYRLLARVDGSDVRLVTRNGNDWS
                     SRMKGLVDAVRDLGIGSA"
     gene            1177..2406
                     /locus_tag="VFF72_05310"
     CDS             1177..2406
                     /locus_tag="VFF72_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZV92613.1"
                     /translation="MNIALLVSWWQPAAVALVAALVALLVYRLVWPLAGRLVRRSPTL
                     QAVLQRLQGPLHWVVPLVVLEIVLEGVPETLPALDGIQHLTGVLLIIAITATAIGAVR
                     GVADAVVLRHPRDVADNLQARRILTQTRVLSRIAIGILAFAGLAFVLMTFPKARQFGA
                     SLLASAGLSALVVGLAARSVFGNLLAGLQIALSQPIRIDDVLIVENEWGRVEEITSTY
                     VVLKIWDERRMVIPLQWFIEHPFQNWTRTSSQIIGSVMFWVDYTTDLEPLREEARRLA
                     QGSKDYDGRVCLLQVVDTNERAMQLRLIVSSPSAGQSWDLRCLMREGVIAFLQRTQPQ
                     ALPRVRAEIDALASPPARPDEPTAPGRAQPARPLPQSTKAREPGEPKAAVNDDTGMAS
                     LNAPTEKKDQVVEPDTA"
     gene            2661..>3005
                     /locus_tag="VFF72_05315"
     CDS             2661..>3005
                     /locus_tag="VFF72_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV92614.1"
                     /translation="MTDLFSLAGRTALVTGGSRGIGRMIAAGFLKQGARRVYIASRKA
                     ADCDAAANELAAFGDCVSLPADVSTLDGVNALAAALGARETAGRLDILVNNAGAAWGE
                     GFDTFPEKGWDKV"
BASE COUNT          390 a         1084 c         1040 g          491 t
ORIGIN      
        1 cgccgagccg atgccgagat cgcgaaccgc gtcgacaagc cccttcatcc gtgacgacca
       61 gtcgttgccg ttgcgagtga cgaggcgcac gtcgctgccg tcgacacgcg ccagcagtcg
      121 atagccgtcg aacttgatct cgtaggtcca gccgtcgtcg tcgggcgcct gcgcgacgag
      181 cgtggcgagc tgcggcgaga gcgtcgccgg cagcgccgcc ttcttcgcgc cgtcggggac
      241 tgccggatgt gccggtcgcg acggccgtgc cggcgcgcgg gcgccggcgg ccaccggctt
      301 cgacgccgtc ccggccgtcg agcttcgcgg tgtcttcggc gtcgatgtcc tcgtcctcgt
      361 cgccggtctc gttgccgtcg ccgtcgtcgc ctttgtcgtc gctttcgttg tcgccgtggc
      421 cgtcgatggt cccgacgtcg cgcccgtcgc ggcgcccgac ttcgacgtcg acttgcgggt
      481 cgcggtcggc gcagggtccg ggatcgtgcg gtcggagagg acgctcgcgg gttcggcatc
      541 gacgacgctg tactgcgagg ccggccgcgc cgattcgtcg cgctccttga tgagcagcca
      601 cggatcctgc cgctcggtcg cgcggccgtg catgcgcacc agcgtccagc cgcccgagag
      661 cttgtggccg tgcaaccgga acttgagctt gccggcggcg tagccggcct gcgcatcgcc
      721 gactggctcc cacgtgccgt tgtcccagac gatgacggtg ccgccgccgt actggccctt
      781 cgggatgtcg ccctcgaagc cggcgtaggc catcggatgg tcttcgacgt gcacggccat
      841 ccgcttgacg gtcgggtcga ggctcggtcc cttgggtacg gcccagctct tcagcgtgcc
      901 gccaagctcg agacggaagt cgtagtgcag gtggctggca gcgtgcttct ggacgacgaa
      961 ggcgagctgt cccgacttcg accgcttgcc gccgcgcggc tcgcgggtga tcgagaagtc
     1021 gcgtttgcga agatacgggc gaagcgcgtc ggccatggcc agcgccgagc aatcgacgtg
     1081 ccgcggccgt ccgccatcga tgtcgatgcg ggcctgccac cggtcgcaat gtcattgcgc
     1141 gtcgcccgcg aatcgacgag ccggtaccct tggccgatga acattgctct tctcgtgtcc
     1201 tggtggcaac ccgccgccgt cgctctggtg gccgcgctcg tcgcccttct cgtctatcgc
     1261 ctcgtctggc cgctcgccgg ccgcctcgtt cgccgctcgc cgacattgca ggccgtcttg
     1321 cagcggctgc agggtccgtt gcactgggtc gtgccgctgg tggtgctgga gatcgtcctg
     1381 gaaggcgttc cggagacgct gccggctctc gacggcatac agcatctcac gggcgtgctg
     1441 ctgatcatcg ccatcaccgc gacggcgatc ggcgcggtcc ggggcgtcgc cgacgcggtg
     1501 gtgcttcgcc acccgcgcga cgtcgccgac aacctgcagg cgcgccgcat cctgacgcag
     1561 acgcgggtgc tttcgcgcat cgccatcggc atcctcgcct tcgccggcct cgccttcgtg
     1621 ctcatgacct tcccgaaggc gcgccagttc ggcgccagtc tgctcgcctc ggccgggctg
     1681 tcggccttgg tggtcggcct ggccgcgaga tcggtcttcg gcaacctgct cgccggcctg
     1741 cagatcgcgc tctcgcagcc gatccgcatc gacgacgtgc tgatcgtcga gaacgaatgg
     1801 ggtcgcgtcg aggagatcac cagcacctac gtcgtgctca agatctggga cgagcgtcgg
     1861 atggtgattc cgctgcagtg gttcatcgag catccgttcc agaactggac ccgcaccagc
     1921 tcgcagatca tcggcagcgt catgttctgg gtcgactaca ccaccgatct cgagccgctg
     1981 cgcgaggaag cgcggcggct ggcgcagggc tcgaaggact acgacgggcg cgtctgcctg
     2041 ctccaggtcg tcgacaccaa cgagcgcgcc atgcagttgc gcctgatcgt cagctcgccg
     2101 tcggccggcc agagctggga cctgcgctgc ctgatgcgcg aaggcgtgat cgccttcctg
     2161 cagcgcaccc agccgcaggc gctgcctcgc gttcgcgccg aaatcgatgc gctggcgtcg
     2221 ccgccggcca ggccagacga gccgaccgcg cccggccgcg cgcaacccgc caggccgttg
     2281 ccgcaatcga cgaaggcgcg cgagccaggc gagccgaagg ccgccgtcaa cgacgacacc
     2341 ggcatggcct cgctgaacgc gcccacggaa aagaaggacc aggtcgtcga gcccgatacc
     2401 gcctgagcgg ggcccgcgcg tcacggcagg cgccgccgcg cgtcgcagcc ggcaacccga
     2461 gcggaacctc ctgagccgcg cgcgccggat ccgtggggcc gctggcaacg aatcccggca
     2521 gggggtcgac ccgggctttc cgcgggcggt cgccctggac gccggcgggc ggtctggccg
     2581 agcctctaca gtgcgggagg gctcgcccga gccgttccgg gcgccttcga tgccgcccct
     2641 ctgtcgccgc tggagttgcc atgaccgatc tcttttccct cgccgggcgc accgccctcg
     2701 tcaccggtgg atcgcgcggc atcggccgca tgatcgccgc cggattcctg aagcaaggcg
     2761 ccaggcgcgt ctacatcgcg tcgcgcaagg ccgccgattg cgacgccgcc gcgaacgagc
     2821 tggcagcgtt cggcgactgc gtgtcgctgc cggccgacgt ctcgacgctc gacggcgtga
     2881 acgccctggc cgccgcgctc ggcgcgcgcg aaacggccgg caggctcgac atcctcgtca
     2941 acaacgccgg cgccgcctgg ggcgagggct tcgacacctt tcccgagaag ggctgggaca
     3001 aggtg
//
LOCUS       DASRWS010000287         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2699147, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000287 DASRWS010000000
VERSION     DASRWS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2699147"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFF72_05320"
     CDS             <1..370
                     /locus_tag="VFF72_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZV92615.1"
                     /translation="FGIRCVVAPGFSDIFFENAFKNGLLPVILPDRDVQELLDFLETT
                     NDPSLTVDLERRVIETLQGRTISFTIPEARRTALLEGLDEIGQTLRQEAEIAAFQARD
                     REARPWIHRRERGLVAKARG"
     gene            372..1256
                     /locus_tag="VFF72_05325"
     CDS             372..1256
                     /locus_tag="VFF72_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HZV92616.1"
                     /translation="MAGHKSLRQIAAEERPLVTPVAHDALAARLIARAGFKAIAIGGS
                     SMLAARYGLPDLGLAALGEMVDGAREIVAATDLPCIIDADDGYGDVKSVARTMELYQR
                     LGVAAIILEDQARDAKQPGDAKARGLVPEAIVEQKIAAAGAARSDPDLLIIARTDSYG
                     VEGLDGALRRGERYLKAGADGIFIPAIAKPEEIERAGAAFRGTYQMIVMSEGGKTPWL
                     SPGELHGMGYSQVAYPSYVVLRSVLAIESALADLKSIAQGRKSPVAWPDYDRARAVFQ
                     DAVRQQQWEEFELRHGRA"
     gene            1243..>3428
                     /locus_tag="VFF72_05330"
     CDS             1243..>3428
                     /locus_tag="VFF72_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZV92617.1"
                     /translation="MVGRDATTPVGVTLDDKYTLDRGRIYLTGIQALVRLPMMQRQLD
                     LAAGLNTGGFISGYRGSPLGGYDTALWAARKHLERANIHFQPGLNEDLAATSLWGSQQ
                     IGMFPGATVDGVFGIWYGKGPGVDRSMDVLKHANLAGTSPRGGVLAIAGDDHACQSSS
                     IPNSSDQVLVAAMIPVLNPASLQDYLDFGLFGFALSRYSGCWVGFKAVGQTVESSASI
                     LVDPDRSRFVLPEDFVIPAGGLGIRWPDPPLEQEARLHGAKMDAVAAFARVNRVDRIM
                     LDSPRPRLAIFTTGKAYADVRQALSDLGIDDARAGALGLRLYKIGLAWPLEPEGARHA
                     ASGVEEVLVVEEKRPLIEDQLVKLLYRVDASRRPEVVGKRDETGGALLPSVGELDPVV
                     VAQAVLARLSRLHGDLPELAQRLAVIEAYQRSATAPGTGAAQRLPFFCAGCPHNTSTK
                     LPDGSRATAGIGCHGMVMWMPYRHTAMTTHMGGEGLMWVGQAPFTSESHVFQNLGDGT
                     YTHSGSLAIRAAVAAGVNITYKVLYNDAVAMTGGQLPEGHMSVPQIARQLAAENVQRI
                     AIVTDEVDKYPVGANFPPGVAIRHRDELDAVQRELRAVPGVTVLIYDQTCAAEKRRRR
                     KRGSFPDPDKRVFINEAVCEGCGDCSEQSHCIAVVPLETELGRKRAIDQSSCNKDFSC
                     LKGFCPSFVTVYGGTPRRPSVEQRSAGLPPLAEPAQPSLDQPYSILV"
BASE COUNT          575 a         1161 c         1112 g          580 t
ORIGIN      
        1 cttcggtata cggtgcgtcg tcgcgccggg cttcagcgat atcttcttcg aaaacgcgtt
       61 caagaatggt ctgctccctg tgattctacc ggaccgcgac gttcaggaac tcctggactt
      121 ccttgagacg accaatgacc cgtccctcac cgtcgatctc gagcgtcgcg tcatcgagac
      181 gctgcaaggc cgcaccatct ccttcaccat cccggaggca cgacggacgg cactgctgga
      241 agggctcgac gagatcggcc agaccttgcg tcaagaggcc gagattgccg cattccaggc
      301 gcgcgaccgc gaggcgcggc cgtggattca tcgtcgcgag cgcggcctcg ttgcgaaagc
      361 gaggggctaa gatggccggg cacaaatccc ttcgccagat cgccgccgaa gagcggcctc
      421 tcgtgacgcc ggtggcgcac gacgcgttgg ccgctcgcct catcgcgcgc gccggcttca
      481 aggcgatcgc gatcggcggc tcatcgatgc tggcggcgcg ctacggcctc cccgatcttg
      541 gcctggccgc gctgggcgag atggtcgacg gcgcgcgcga gatcgtcgcg gcgaccgatc
      601 ttccatgcat catcgacgcc gatgatggct atggcgacgt gaagagcgtt gcgcgaacga
      661 tggagctcta tcaacggctc ggcgttgccg cgatcattct cgaggatcag gcgcgtgacg
      721 ccaagcagcc gggcgacgcc aaggcacgcg ggctggtacc cgaggcaatc gtcgagcaga
      781 agattgccgc cgccggcgca gcgcggtccg atccagacct tctcataatc gcccgcacgg
      841 actcttatgg tgtcgagggg ctcgatggcg ccttgcgtcg cggtgagcgc tatttgaagg
      901 caggtgccga cgggatcttc attccggcga tcgcaaagcc cgaagagatc gagcgtgcag
      961 gcgccgcgtt ccgcggcacc taccagatga tcgtcatgag cgagggcggg aagacgccat
     1021 ggctttcgcc cggcgagctg cacggtatgg gctacagcca agtcgcatac ccgagctatg
     1081 tcgtgctgcg cagcgtgctg gccatcgaga gcgcgctcgc cgatctcaag agcatcgccc
     1141 aaggtaggaa gtcgcccgtc gcttggcccg attacgaccg tgctcgagcg gtctttcagg
     1201 acgccgtacg gcagcaacaa tgggaagagt tcgagctacg tcatggtcgg gcgtgatgcg
     1261 acgacgccgg tcggcgtcac gctcgacgac aagtacacgc tggatcgcgg ccgcatctat
     1321 ctgaccggca tccaagcgct cgtgcggcta ccgatgatgc agcgccagct cgaccttgcg
     1381 gcgggactca ataccggcgg cttcatctcg ggctatcgcg gctcgccgct tggcggttac
     1441 gacaccgccc tctgggcggc acggaagcat ctcgaacgcg ccaacatcca tttccagccg
     1501 ggcctcaacg aagacctcgc ggcgacatcg ctctggggaa gccagcagat tgggatgttt
     1561 cctggtgcaa cggtggacgg cgtattcggt atttggtacg gcaaaggacc gggcgtcgac
     1621 cgttcgatgg acgtcctgaa gcacgccaac ctcgccggca cttcgccgcg tggcggcgtt
     1681 cttgccattg ccggcgacga tcatgcctgc cagtcatcct cgatcccgaa cagtagcgac
     1741 caggtcctgg tcgcggcgat gatcccggtg ctcaatccgg caagcctgca ggactatctc
     1801 gacttcggtc tcttcggctt cgccctctct cgctactcgg gttgctgggt cggcttcaag
     1861 gcggtcgggc agaccgtcga gagttcggcc tccattctgg tggacccgga ccgctcgcgc
     1921 ttcgtcctgc cggaggattt cgtcattccg gccggcggtc ttggaatccg ctggcccgat
     1981 ccgccgctcg agcaggaggc gaggctgcac ggcgccaaga tggacgctgt cgccgccttc
     2041 gccagggtca atcgcgtcga ccggatcatg ctcgactcgc cccggccgcg ccttgcgatc
     2101 ttcaccaccg gcaaagccta tgccgatgtc cgccaggcgc tcagcgatct cgggatcgac
     2161 gacgcccgcg ccggcgcgct gggcctgcgt ctctacaaga tcggcctggc ctggccgctc
     2221 gaacccgaag gcgcgcgcca cgccgcctcg ggcgtcgagg aggtgctcgt cgtcgaggag
     2281 aagcgcccgc tgatcgagga tcaactggtc aagctgctgt accgggtaga cgcatcgcga
     2341 cgccccgagg tggtcggcaa gcgcgacgag accggaggag ccctgctgcc gagcgtcggt
     2401 gagctcgacc cggtcgtcgt ggcgcaggcc gtcctcgcgc gcttgagccg cctgcacggc
     2461 gatctgccgg agctggcgca acgcctcgcc gtcatcgagg cctaccagcg aagcgcgacc
     2521 gcgcccggca ccggcgcggc gcagcgctta cccttcttct gcgccggttg cccccacaat
     2581 acctcgacaa agctgccgga cggcagtcgt gcgacggccg gtatcggctg ccacggcatg
     2641 gtcatgtgga tgccctatcg ccatacggcg atgacgacgc atatgggcgg cgaaggcttg
     2701 atgtgggtcg gccaggcgcc gttcaccagc gagagccacg tcttccagaa cctcggggac
     2761 ggcacctata cgcattcggg ctcgctggcg atccgcgcgg cggtggctgc cggggtcaac
     2821 atcacctaca aggtgctcta caacgatgcc gtggcaatga ccggcgggca attgccggaa
     2881 ggccacatgt ccgtgccgca gattgcccgt cagctcgccg ccgagaacgt ccagcgcatc
     2941 gccatcgtga ccgacgaggt cgacaagtac cctgtgggtg cgaacttccc gccgggcgtc
     3001 gccatccgcc atcgcgacga gctcgacgcg gtgcagcgcg agctgcgcgc ggttcccggc
     3061 gtcacggtcc tgatctacga ccagacctgc gccgccgaga agcgccgccg gcgcaagcgc
     3121 ggcagcttcc ccgatcccga caagcgcgtc ttcatcaacg aagccgtctg cgagggttgc
     3181 ggcgattgct ccgagcagtc gcactgcatc gccgtagtac ctctcgaaac cgagctcggg
     3241 cgcaagcggg ccatcgatca gtcgagctgc aacaaggatt tctcctgcct taagggattc
     3301 tgcccgagct tcgtcactgt ctatggcggc acaccacggc gacccagcgt cgagcagcgt
     3361 tcggccgggc tgccgccgct cgccgagccg gcccagccat cgctcgacca gccatacagc
     3421 atcctcgt
//
LOCUS       DASRWS010000288         5499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1563641, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000288 DASRWS010000000
VERSION     DASRWS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563641"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFF72_05335"
     CDS             <1..308
                     /locus_tag="VFF72_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZV92618.1"
                     /translation="HLSRGEAEAFYAVHKARPFFNDLVSFMISGPVVIQVLEGSDAIA
                     KNRQLMGATDPKKADKGTIRADFADSIDANAVHGSDAAETAAIEIAFFFAGMNVYGR"
     gene            328..1524
                     /gene="rlmN"
                     /locus_tag="VFF72_05340"
     CDS             328..1524
                     /gene="rlmN"
                     /locus_tag="VFF72_05340"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZV92619.1"
                     /translation="MVNLLQFDRPGLADFCAELGEKRFRATQLFRWIHQRGESDLAAM
                     SDLARPLRDKIAAAAGVLPLPLQSEQRAGDGTVKWLFDVGGGDAVETVFIPETGRGTL
                     CISSQAGCAVNCRFCATGHQGFSRNLTTGEIVGQLWHAEFDLRRRLGLPPGERAITNV
                     VMMGMGEPLQNYAALVPALRTMLDDHGYGLSRRRVTVSTSGVVPMIDRLREDCPVALA
                     VSLHAADDALRDELVPINRKYPIAELLAACRRYLDRAPRDFVTVEYCMLDGVNDSQAQ
                     AEALLALLRGDGSAQRPGLSCKVNLIPFNPFAESGLHRSPPERVAAFASVLQEGGIVT
                     TVRKTRGDDIAAACGQLAGEVKDRTRVVDRRRQSMGELSEHRMSGPVSRRDAAGRSHR
                     PERILP"
     gene            1521..2360
                     /gene="pilW"
                     /locus_tag="VFF72_05345"
     CDS             1521..2360
                     /gene="pilW"
                     /locus_tag="VFF72_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HZV92620.1"
                     /translation="MTLGRFFRLVIGAPVAAAALAAAMTGCVTTTTSSTQSAAASDNH
                     DIATSADEANPQKRARARVDLAAAYFGRGQMTIALDQVKLALAADPNYGQAYNLRGLI
                     FANLGDDVRAEESFKRALQIDPKDGDTLHNYGYYLCEHKRYAESTTLFRQALTVQQYR
                     GTSRTLLALGVCQARAGDLAGADASLSRAYELDTGNPVISSNLAEVLYRRGQYERARF
                     YIRRVNAMPNVSNAHTLWLAARIEHRLHDDRGATDFGVQLRNRFPDSREADSFARGAF
                     DDE"
     gene            2353..3285
                     /locus_tag="VFF72_05350"
     CDS             2353..3285
                     /locus_tag="VFF72_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZV92621.1"
                     /translation="MNEEAAQGVEKPTAGRLLREAREKQGLHIAALAASIKVAPKKLE
                     SLESDRFDELPDATFARALAQTVCRALKIDAAPIMRLLPPPSGHRLEQVGEGLNTPFR
                     DRPGLAVQGDWQAFAMNPWAWVVLSILVAAAVVYLLPGRWIDLGAARLRPSASASSVV
                     EPGMPPEQREAAASAPLTSPAPIVTGTAPAATRPEMPPAAAAETAGDTASGPASTDAA
                     STPVASSSSAAASPSAVLQFRSTGPSWIEVTDAQGKTLISRVVGAGETIGLDGTAPFK
                     LKIGNASATRVTLRGQPVALEAFTRDNVARLELH"
     gene            3297..4562
                     /gene="ispG"
                     /locus_tag="VFF72_05355"
                     /gene_synonym="gcpE"
     CDS             3297..4562
                     /gene="ispG"
                     /locus_tag="VFF72_05355"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZV92622.1"
                     /translation="MRDESISLASFIEPAAPAPRRSRQASVRWGSRVVTVGGDAPVRV
                     QAMTNTDTVDAIGTAIQVKELAIAGSEMVRITVNTPEAAAAVPHIREQLDRMGIDVPL
                     IGDFHYNGHRLLTDFPACAEALSKYRINPGNVGKGDKHDRQFAQMVEVAVRHGKPVRI
                     GVNWGSLDQDLLAELMDENALLAEPQEPRQVMYRAIVLSALSSARRAEEIGLAPEQII
                     LSCKMSGVQDLVSVYRALARQSDYPLHLGLTEAGMGTKGTVASTAALAILLEEGIGDT
                     IRVSLTPQPGEARTQEVVVALEILQSLGLRSFNPSVTACPGCGRTTSTTFQELAKQID
                     DHLRARMPEWKARYPGVETMKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFID
                     GEKAMTLRGDRIAEEFHALVESYIEKRFG"
     gene            4635..>5499
                     /gene="hisS"
                     /locus_tag="VFF72_05360"
     CDS             4635..>5499
                     /gene="hisS"
                     /locus_tag="VFF72_05360"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZV92623.1"
                     /translation="MSEALRAVKGMNDILPPESARWEWFEAKVRDLMRRYGYANVRTP
                     IVEPTALFVRGLGEVTDIVEKEMYSFEDSMNGDRLTLRPEATAGIVRAMVEHNALYNG
                     PLRLWSQVAVFRHERPQKGRYRQFHQIDVEALGHPGPDVDAEQILMCRALLRDLGLVE
                     GRDVRLEINSLGQADERIAHRAALIAHLQQHALDLDEDARRRLQSNPLRILDSKSPAM
                     QAIIEAAPRLIDFLGPQSLAHLDAVRAVLDAAGLAYRVNPRLVRGLDYYNLTVFEWVT
                     DRLGAQGTVCGG"
BASE COUNT          886 a         1888 c         1854 g          871 t
ORIGIN      
        1 cgcatctctc gcgcggcgag gcggaagcgt tctacgccgt gcacaaggcg cgtcccttct
       61 tcaacgacct ggtctcgttc atgatctccg gtcccgtcgt gatccaggtt ctcgaaggca
      121 gcgatgccat cgccaagaac cgccagttga tgggcgcgac cgatccgaag aaggccgaca
      181 agggaaccat ccgtgccgac ttcgccgaca gcatcgatgc caacgcggtc cacggctcgg
      241 acgccgcgga aacggcggcc atcgagatcg ccttcttctt tgccgggatg aacgtctacg
      301 gacgttgaat gaccgtcccc ttccggcatg gtcaacctgc tgcagttcga ccgaccaggg
      361 cttgccgatt tctgcgccga gctcggcgaa aagcgctttc gggcgaccca gctctttcgc
      421 tggatccatc agcgtggcga gtccgatctt gcggcgatga gtgacctcgc gcggcccctg
      481 cgcgacaaga tcgccgctgc tgccggggtg ctgccgcttc ccctgcaaag cgagcagcgg
      541 gccggcgacg gcaccgtcaa gtggctcttc gacgtcggcg gcggcgacgc cgtcgagacc
      601 gtcttcatcc ccgagaccgg ccgcggcacg ctgtgcatct cttcgcaggc gggctgcgcg
      661 gtgaactgcc gcttctgcgc caccggtcac caggggttca gccgcaacct gacgaccggc
      721 gagatcgtcg gccagctctg gcatgccgag ttcgacctgc gacgacgcct cggcctgccg
      781 cctggcgaac gagcgatcac gaacgtcgtc atgatgggca tgggcgagcc cctgcagaac
      841 tatgcggcgc tggtgccggc gctgcggacc atgctcgacg accacgggta cggactgtcg
      901 aggcgccgcg tcacggtctc gacgtccggt gtcgtgccca tgatcgaccg gctgcgcgag
      961 gactgtccgg tggcgctcgc cgtgtcgctc catgccgccg acgacgcgct gcgcgacgaa
     1021 ctcgtgccga tcaatcgcaa atatccgatc gccgagttgc tcgctgcctg ccgtcgctac
     1081 ctggaccggg cgccgcgcga cttcgtgact gtcgagtact gcatgctgga cggcgtcaac
     1141 gactcgcaag cgcaagccga ggcgctcctc gcgttgcttc ggggcgatgg cagcgcgcaa
     1201 cggccgggac tgtcgtgcaa ggtgaacctg attcccttca atccgttcgc ggagtcgggg
     1261 ttgcaccgat cgccgccgga gcgagtcgct gcattcgcat cagtcctgca ggaaggtggc
     1321 atcgtgacca ccgtgcgcaa gacgcgcggc gacgacatcg ccgccgcctg tggccagctg
     1381 gccggcgagg tgaaggatcg aacccgcgtc gtcgatcggc gccgtcagtc gatgggcgag
     1441 ctctccgagc accggatgtc cggcccggtg tcgcgtcgcg atgccgccgg gcgcagccat
     1501 cgcccggagc gcatcctgcc atgaccctgg gccgattctt tcgtctggtc atcggcgcgc
     1561 cggtcgctgc cgcagccttg gccgcggcga tgaccggatg cgtgacgacc acgacttcgt
     1621 cgacgcagtc ggcggcggcg tcggacaacc acgacatcgc cacgtcggcc gacgaggcca
     1681 atccgcaaaa gcgggcgcgc gcccgcgtcg atctggcggc ggcgtacttc gggcgtggcc
     1741 agatgacgat cgccctcgat caggtgaagc tcgccctggc cgcggatccg aactacggac
     1801 aggcctacaa cctgcgcggg ctgatcttcg ccaatctcgg cgacgacgtc cgtgccgaag
     1861 agagcttcaa gcgagcgctg cagatcgatc cgaaggacgg cgacacgctg cacaactacg
     1921 gctactacct ctgcgagcac aagcgctacg ccgagtcgac gacgctcttc cggcaggcgt
     1981 tgacggtgca gcagtaccgg ggcacgtccc ggacgcttct ggcgctcggt gtctgccagg
     2041 cacgcgcggg ggatctggcg ggggccgacg cttcgctctc gcgcgcctac gaactcgata
     2101 ccggcaaccc ggtcatctcg agcaatcttg ccgaggtcct ctaccgccgc ggccagtacg
     2161 aacgggcacg cttctatatc cggcgcgtca acgcgatgcc caacgtctcg aatgcccata
     2221 cgctttggct ggcggcgcgg atcgagcacc ggcttcacga cgatcgcggc gccaccgact
     2281 tcggcgtgca gttgcgcaac cggtttcccg actcccggga ggccgattcg ttcgcgcgtg
     2341 gtgctttcga cgatgaatga agaggcggcg caaggcgtcg agaagccgac cgccgggcgc
     2401 ctccttcgcg aggctcgaga gaagcagggc ctgcacatcg ccgcgctcgc ggcgtcgatc
     2461 aaggtggcgc cgaagaagct cgagtcgctg gagtcggatc gcttcgacga actgcccgac
     2521 gcgactttcg cgcgggccct ggcgcaaacc gtctgccggg cgctcaagat cgacgcggcg
     2581 ccgatcatgc ggctgctgcc cccgccttcg gggcatcgac tcgagcaggt cggcgaaggc
     2641 ctcaacacac cctttcgcga ccggcccggc ctggcggtgc agggcgactg gcaggccttc
     2701 gcgatgaatc cgtgggcgtg ggtggtcctg tcgatcctcg tcgcggccgc cgtggtctac
     2761 ctgctgcccg gccgctggat cgacctcggc gcggcgcgcc ttcgaccctc ggcgtcggcc
     2821 tcgtccgtcg tcgaacccgg catgccgccc gagcagcgcg aggccgcggc gtcggcgccg
     2881 ctcacctcgc ccgcgcccat cgtcacgggc accgcgcccg cggcgacgcg tccggagatg
     2941 ccaccggcgg cagccgcgga gacggcgggc gatacggcgt cggggccggc gagcaccgat
     3001 gcggcgagca cgcccgtggc gtcgtcgtcg tcggccgctg cgtcgccctc cgccgtgttg
     3061 caattccgca gcaccggacc ctcctggatc gaagtgaccg atgcgcaagg caagaccctg
     3121 atatcgagag tggtcggcgc cggcgagacg atcggcctcg acggcacggc gcccttcaag
     3181 ctgaagatcg gcaatgcctc ggcgacgcgg gtcacgttgc gcggccagcc ggtggctctc
     3241 gaggccttca cccgcgacaa cgtggctcgg ctcgaacttc actgaggagc gacaacatgc
     3301 gcgacgaaag catttcactc gcatcgttca tcgaacctgc ggcgccggcg ccgcgccggt
     3361 cgcgccaggc cagcgtgcgc tggggctcgc gcgtcgtgac cgtcggcggc gacgcgccgg
     3421 tgcgcgtcca ggcgatgacc aacaccgaca cggtcgacgc gatcggcacc gccatccagg
     3481 tcaaggaact ggcgatcgcc ggctccgaga tggtccgcat caccgtcaac acgccggagg
     3541 ccgccgcggc ggtgccgcac attcgcgaac aactcgaccg gatgggcatc gacgtgccgc
     3601 tgatcggcga tttccactac aacggccatc gccttctgac cgacttcccg gcgtgtgccg
     3661 aggcgctgtc gaaataccgc atcaatcctg gcaacgtcgg caagggcgac aagcacgacc
     3721 ggcagttcgc gcagatggtc gaagtggccg ttcgccacgg caagccggtg cgcatcggcg
     3781 tcaactgggg cagtctcgac caggacctcc tcgccgagct gatggacgag aacgccttgc
     3841 tcgccgagcc gcaggagccg cgccaggtga tgtaccgggc gatcgtcctt tccgcgctgt
     3901 cgtcggcgcg gcgggccgag gagatcggcc tggcgccgga gcagatcatc ctgtcgtgca
     3961 agatgtcggg tgtccaggac ctcgtcagcg tctatcgtgc gttggcacgc caatccgact
     4021 acccgcttca tctcggactc accgaagcgg gcatgggcac caagggcacc gtcgcttcga
     4081 cggcggcgct ggcgatcctc ctcgaagaag gcatcggcga caccattcgc gtctcgctga
     4141 cgccgcagcc gggcgaagcg cgcacgcagg aagtcgttgt cgccctggaa atcctgcagt
     4201 cgctcggcct gcgctcgttc aacccgagcg tcacggcatg tccgggttgc ggccggacga
     4261 cgagcacgac cttccaggaa ctggccaagc agatcgacga ccaccttcgc gccaggatgc
     4321 cggagtggaa ggcacgctat cccggcgtcg agacgatgaa ggtcgccgtg atgggctgca
     4381 tcgtcaacgg tccgggcgag agcaagcatg ccgacatcgg catcagtctt cccggaaccg
     4441 gcgaagcgcc ggcggcgccc gtgttcatcg acggcgagaa ggcgatgacg cttcgcggcg
     4501 accggatcgc ggaggaattc catgcgctcg tcgaaagcta catcgagaag cgcttcggct
     4561 gacgccatga cgtccggtgg ccggtcgcgg cgatacccgt tctgctgccg cggcgagcaa
     4621 ggaatcgtct tgtcatgagc gaggcgctgc gagccgtcaa gggcatgaac gacatcctgc
     4681 cgccggagtc ggcgcgctgg gagtggttcg aggcgaaggt gcgcgacctg atgcggcgct
     4741 atggctatgc caacgtgcgc acgccgatcg tcgagccgac ggcgctcttc gtgcgcggcc
     4801 tcggcgaggt gaccgacatc gtcgagaagg agatgtattc cttcgaggat tcgatgaacg
     4861 gtgatcgcct gacgctgcgg ccggaagcga cggcgggcat cgtccgcgcg atggtcgagc
     4921 acaacgccct ctacaacggc ccgttgcggc tgtggagcca ggtggccgtg ttccgccacg
     4981 agcggccgca gaagggtcgc tatcgtcagt ttcaccagat cgatgtcgaa gcgctcggcc
     5041 atcccggccc cgacgtcgac gcggagcaga tcctgatgtg ccgcgcgttg cttcgcgacc
     5101 tcggcctggt cgaagggcgc gacgttcgtc tcgagatcaa cagccttggc caggcggatg
     5161 agcgcattgc gcaccgtgcg gcgttgatcg cgcacctgca acagcatgcc ttggacctcg
     5221 acgaggacgc ccggcgtcgc ctgcagagca atccgcttcg catcctcgac agcaagagcc
     5281 cggcgatgca ggcgatcatc gaggcggcgc cgcgcctgat cgattttctc gggccgcagt
     5341 cgctcgcgca tctcgacgcg gtccgcgccg tgctcgacgc cgcggggctg gcgtaccggg
     5401 tcaatccacg tctcgtacgc gggctcgact actacaacct gacggtcttc gaatgggtga
     5461 ccgatcgcct cggcgcgcag ggcaccgtgt gcggtggcg
//
LOCUS       DASRWS010000289         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_117603, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000289 DASRWS010000000
VERSION     DASRWS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117603"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="VFF72_05365"
     CDS             <1..158
                     /locus_tag="VFF72_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZV92624.1"
                     /translation="NPHIGYDKSAQIAKEAHRSGSTLREAAVASGWLSGEQFDAWVDP
                     RRMTGRS"
     gene            155..832
                     /gene="rpiA"
                     /locus_tag="VFF72_05370"
     CDS             155..832
                     /gene="rpiA"
                     /locus_tag="VFF72_05370"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HZV92625.1"
                     /translation="MNALSKKDQDELKRQVGLAAVEQVEAGSIVGVGTGSTVNCFIDA
                     LGAVRDRVAGAVSSSEASTARLRALGVRVLAAGDVERLGVYIDGADEIDHAGCMIKGG
                     GGALTREKIVADLADRFVCIADESKLVDRLGRFPLPIEVVPMAVAQVSRRFVALGGDP
                     RVREGFVTDNGGVIIDVHGLAIDDPLRLESEIDQWAGVVCCGIFAQHRASVCLLGTSG
                     GVRTLRF"
     gene            912..1097
                     /locus_tag="VFF72_05375"
     CDS             912..1097
                     /locus_tag="VFF72_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92626.1"
                     /translation="MAKPNYSFEKRQRELAKKKKKEEKEKEKAGRKLAADLPAEAANG
                     QAADQDPADPPAVGPAG"
     gene            complement(1055..>2804)
                     /gene="mnmC"
                     /locus_tag="VFF72_05380"
     CDS             complement(1055..>2804)
                     /gene="mnmC"
                     /locus_tag="VFF72_05380"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HZV92627.1"
                     /translation="LAAVPRDPIVMPLAQQLVDDWPPHTFNLHRLPFEDGRVELLLAF
                     GDARDWLPQIDAAVDAFFLDGFAPAKNPQLWEPRLFKSMGRIAVPEATAATWSAAKPV
                     RDGLRAAGFDARRADGRDGKRDITVARYAPAFATRLRDRRRPATPSRSGDPVLVVGAG
                     LAGCATACALAEHGLASIVIDRTEAIASEGSANPGGLFHGVVHRDDARHARWHRAAAF
                     EARRQVADAIASHGVAGGLDGLLRLEIPVQAGHFDACVARMKDTVLRLGFPASYVAPL
                     TPSQASAHGGVPVAVPAWFFPGGGWVDPRGLARAYIDRAGSSVELRLDTTVAALRIDD
                     DGRWLLLDPAGRTIASSPHVVLANGGGALELLGANDCTGLIEKRRGQLSAAPTSALPL
                     QARVRLPIAGAGYVLPPIDGRVWFGATSDLEDGDAPRENDHRRNVERLRHLLPYAPLL
                     SPDKLDDRVAFRYSTRDRLPLIGAVPAAWLGPALREKRAIADSRHPEQVRSAARAPGL
                     FMLSALGSRGIAGSALGARLVAAAIGGAPMTIEADLLDAIDPARFACRAWRRGRGGVA
                     SLDQPAGPTAGGSAGS"
BASE COUNT          474 a          940 c         1005 g          385 t
ORIGIN      
        1 tcaatccgca catcggctac gacaagtcgg cgcagatcgc caaggaggcg catcgcagcg
       61 gctcgacgct tcgcgaggcc gcggtcgcgt ccggctggct gagtggcgag cagttcgatg
      121 cctgggtcga cccccgacgg atgacaggcc gctcatgaac gctctttcga agaaggatca
      181 ggacgagctg aagcggcagg tcgggctggc ggccgtcgag caggtcgagg cgggcagcat
      241 cgtcggcgtg ggaaccggct cgaccgtcaa ctgcttcatc gatgccctcg gcgcggttcg
      301 cgaccgcgtc gccggcgccg tctcgagcag cgaggccagc acggcccggc tgcgcgccct
      361 cggcgtccga gtgctcgctg ccggcgacgt cgaaaggctc ggtgtctaca tcgacggtgc
      421 cgacgagatc gatcatgcgg gctgcatgat caagggcgga ggcggtgcgc tgacccggga
      481 aaagatcgtc gccgatctcg ccgatcgatt cgtctgcatc gccgacgaat cgaagctcgt
      541 cgatcggctc ggccgctttc cgctgccgat cgaggtggtg ccgatggccg tggcgcaggt
      601 atccaggcgg ttcgttgccc tgggcggcga tcctcgcgtg cgtgagggct tcgtcaccga
      661 caacggcggc gtcatcatcg acgtgcacgg cttggcgatc gacgacccgc tccggctcga
      721 gtccgagatc gatcaatggg ccggcgtggt gtgttgcggc atcttcgcgc aacaccgggc
      781 gagcgtctgc ctgctcggca cgtcgggagg cgttcgaacc ttgcgctttt gacaagcggt
      841 gacggttcgc cgggcaaaag cgcgtaacct cgggctcctg cacgtcttgc agcactgagg
      901 aaggacggcc cgtggccaaa ccgaactatt cattcgagaa acgacaacga gaactcgcca
      961 agaagaagaa gaaggaagag aaggaaaagg aaaaagccgg ccgcaagctc gcggccgacc
     1021 tgcccgccga agcggccaac gggcaggctg ccgatcagga tcctgccgat ccgccggccg
     1081 tgggcccggc gggctgatcg agggacgcga cgccgccccg gccgcgccgc caggcgcggc
     1141 acgcgaaccg ggccgggtcg atcgcgtcga gcagatcggc ctcgatggtc atcggcgcac
     1201 cgccgatcgc cgccgcaacg agtcgagcgc caagcgccga tcctgcgatc ccccgcgagc
     1261 cgagagcgga aagcatgaag agcccgggcg cgcgtgcagc cgaccgcacc tgctccgggt
     1321 gcctggaatc cgcaatagcc cgtttctccc gcaaggccgg accgagccag gccgccggca
     1381 ccgcgccgat caggggcagc cgatcgcgcg tcgagtaacg aaaggcaaca cgatcgtcga
     1441 gcttgtctgg cgacagcaac ggcgcgtacg gcagcaagtg ccggagccgt tcgacgttgc
     1501 ggcgatggtc gttttcgcgt ggcgcatcgc cgtcctcgag gtcgctcgtg gcaccgaacc
     1561 atacgcggcc gtcgatcggc ggcaacacgt aaccggcgcc agcgatcggc aatcgcaccc
     1621 gcgcttggag gggaagggcg ctcgtcggcg cggcgctcag ctggccgcgg cgcttttcga
     1681 tcagaccggt gcagtcattg gcaccgagca gttcgagcgc tccgccccca ttggcgagca
     1741 cgacatgagg tgacgaagcg atcgtcctgc ctgccggatc gagaagcagc cagcgaccgt
     1801 cgtcgtcgat gcgcagggcg gcgacggtcg tgtccaggcg gagctcgacc gacgagccgg
     1861 cgcgatcgat gtatgcgcgc gccaggccgc gcgggtcgac ccagccgccg cccgggaaga
     1921 accacgctgg caccgcgacg ggtactccgc cgtgagcgct ggcctgcgag ggcgtcaggg
     1981 gcgcgacgta agaggccggg aagccgaggc gcaagaccgt gtctttcatc cgggcgacgc
     2041 aggcatcgaa gtggcccgct tgcaccggaa tttcgagtcg caggaggcca tcgaggccgc
     2101 cggcgacgcc gtgcgacgcg atcgcgtcgg cgacctgccg cctcgcctcg aacgccgccg
     2161 cgcggtgcca gcgcgcatgt ctcgcgtcgt cgcgatggac cacgccatgg aacaacccgc
     2221 caggattggc ggagccttcg ctggcgatgg cctccgtgcg atcgatgacg atcgaagcga
     2281 gaccgtgctc tgccagcgcg cacgctgtcg cacagccggc gagtccggcg ccgaccacga
     2341 gcaccggatc gccggatcgg ctcggcgtgg cggggcgacg gcgatcgcgc aggcgcgtcg
     2401 cgaaggcagg cgcgtagcgc gcgacggtga tgtcgcgctt gccatcgcgt ccatcggcac
     2461 ggcgagcgtc gaagccggcc gcgcgcaagc cgtcgcgcac cggcttcgcg gccgaccagg
     2521 tcgccgcagt cgcctcgggc acagcgatcc ttcccatcga cttgaaaagg cgcggttccc
     2581 agagctgcgg atttttcgcc ggcgcgaagc cgtcgaggaa gaaggcgtca accgcggcgt
     2641 cgatctgcgg cagccagtcg cgggcgtcgc cgaacgcgag caacagttcg acccgcccgt
     2701 cctcgaaggg aaggcggtgc aggttgaagg tgtgcggcgg ccagtcgtcg acgagttgct
     2761 gcgccagcgg catcacgatc gggtcgcgcg gcactgccgc cagc
//
LOCUS       DASRWS010000290         5498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3573413, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000290 DASRWS010000000
VERSION     DASRWS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573413"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="soxY"
                     /locus_tag="VFF72_05385"
     CDS             complement(<1..444)
                     /gene="soxY"
                     /locus_tag="VFF72_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HZV92628.1"
                     /translation="MHMNRRHLLRQGGALALLVSCGVATLDEALAAEASGFDAKTLDD
                     ALASLGAKPADSKDITLTSPDIAENGAVVPLTISSAIPKTDTLYVLVEKNPFPLAASF
                     SIPDGTEPFVAARVKMGESTRVYAVVKADGKLYSTFKETKVTLGGC"
     gene            complement(689..1819)
                     /locus_tag="VFF72_05390"
     CDS             complement(689..1819)
                     /locus_tag="VFF72_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZV92629.1"
                     /translation="MKRPLLVRSFLALALLPLAVAAAPRAPAAPASVPWAGIGRAATP
                     AEVAAWNIDVRGDFQGLPKGAGSVDLGTTVWEAKCASCHGEFAESNQVFTPIVGGTSK
                     KDIESGHTWFLSHPGYPHRTTLMKISHLSSIWDYVNRAMPWNAPKSLKTDEVYGVLAY
                     ILNLGDIVPADFTLSDANIAEVEKRLPNRYGHVFFKSMWQTRGKGDVSNPLCMRDCPV
                     EGRIESSIPAAAMNSNGNIADQVRPFGPERGTDTARIAALAGGGADAAAPSAAGPAPA
                     VAATPQAASIAATPAGVDVQALIKANGCTGCHGIDQKIVGPAFREVAQKHVGSTDLTG
                     FLAARIRNGGQGTWGAIPMPAQPQLSEADARAIAHWIANGAR"
     gene            complement(1834..3159)
                     /gene="soxC"
                     /locus_tag="VFF72_05395"
     CDS             complement(1834..3159)
                     /gene="soxC"
                     /locus_tag="VFF72_05395"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HZV92630.1"
                     /translation="MNLPAMPGRIRKAPENHLDADELERARRARRRFMGAALAMGAGA
                     AVAAQPPIGEGDPNILRLPEHSKGLGLPVASTGYGSPSKWEKNIQRRPSPGLTQVAQA
                     SVSFCPLQSLFGIITPSGLHFERHHQGWWDIDPSKHRLMINGLVEEARVFTMDDLMRL
                     PQVSRIHFIECGANSAMEWGNSAVPSVQYTHGMLSCSEYTGVRLSTLLEMCGADTKRG
                     RYVLAEGADGSSMTRTLPMANALDDILVAWGMNGEMLRPENGYPLRLVAPGIQGVSWV
                     KYLRRIKVGDQPFFTKDEESHYVDLMPDGMLRQFTSIQECKSVITTPSGAQTLLDKGY
                     YNVTGLAWSGRGKVTRVDVSADGGRNWRTARLEGPVLGKALTRFNIDWVWDGSPAILQ
                     SRAVDDTGYVQPKINQLRAVRGSRSIYHNNAIQSWRVSETGDVSNVQAY"
     gene            complement(3172..3654)
                     /locus_tag="VFF72_05400"
     CDS             complement(3172..3654)
                     /locus_tag="VFF72_05400"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZV92631.1"
                     /translation="MLQAIDARKSRGAARAGKAAAAAGGEPTRATGKAVTEPTGGATA
                     ATDEMGEVFESVARYFLVLAEPARLRILHAVCQGERTVGEIVAETGLTQSNASRHLNM
                     MFHLGALRRRRDGAQVFYRVADSTLTDVCRAVCVRVSSELDEKTGLRTGVHGLIERLR
                     "
     gene            3752..4141
                     /locus_tag="VFF72_05405"
     CDS             3752..4141
                     /locus_tag="VFF72_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZV92632.1"
                     /translation="MRPLPTALEARPGGIEIVLFSQADCEFCAEIRDHYLRPLMAERR
                     PAIAVAESRIDADLPIRDWQGRSTTQRLFSAGQGVRFAPTVMFFDASGRSLAPPIVGL
                     STDFFGSYLDQRIAVALAAARRSDNRP"
     gene            4165..4608
                     /locus_tag="VFF72_05410"
     CDS             4165..4608
                     /locus_tag="VFF72_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZV92633.1"
                     /translation="MRPTSRRLFAFGTGLAAAAAALPSARAAAAGEPGRNKVVFQVSD
                     GDPSKWNLTLNNVRNFQTELPDDESDIEIVAYGPGIDMLKGGSSAAHRIAETLKHGVK
                     VVACENSMRGQKLVYADMLPDIGFVPAGVVELVRKQQQGYAYIRA"
     gene            4798..>5498
                     /locus_tag="VFF72_05415"
     CDS             4798..>5498
                     /locus_tag="VFF72_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZV92634.1"
                     /translation="MTEADFQHTTALVLALAFVIGVLFGAIGQRTRFCTMGAISDMAV
                     MGDPARLRMWLVAAGVAIVGFNALAGLGLVRAADSIYAAPRLLWLSALVGGAMFGFGM
                     VLASGCTSKNLMRAGSGNLKSVVVLLVVAFAGFATLKGATAIVRVEWLDSVHVELAGG
                     QDLPHILERLTGVAARPLAVGLGLALGAALLLAGIVPRETRTSDLFVGGIGIGVLIVA
                     AWYVSGVIGFVAENP"
BASE COUNT          752 a         1930 c         1902 g          914 t
ORIGIN      
        1 gcagccgccg agcgtgacct tggtttcctt gaaggtcgaa tacagcttgc cgtcggcctt
       61 gacgacggcg tagacccgcg tgctttcgcc catcttcacg cgggcggcga cgaacggctc
      121 ggtgccgtcg ggaatcgaga agctcgcggc gagcggaaac gggttcttct cgaccagcac
      181 gtagagggtg tcggtcttcg ggatcgcgct cgagatcgtc aggggcacga ccgcgccgtt
      241 ctcggcgatg tcgggcgagg tgagcgtgat gtccttgctg tccgccggct tggcgccgag
      301 cgaggcgagc gcgtcgtcga gcgtcttcgc gtcgaacccc gacgcctcgg cggcgagcgc
      361 ctcgtcgagc gtcgccacgc cgcagctcac gaggagcgcg agcgcgccgc cctggcgcag
      421 gagatgtcgt cggttcatgt gcatcgaaag ttcctctcgt ctcggtgcgc tgtcagcgtt
      481 gcgcagattt tcggaaatgt tcgcggattt cgccccggcg tgccacggca cgaacccgcg
      541 accgcacagc gcctctcgcc tcgacgcgcg gggccgtggt ctcggtcggt ctcatgttcg
      601 ggtcgccgga gtcgttggtc gttggtcgtg gtcgtggtcg tggacgaggt cgaggtcgag
      661 gtcgaggtcg aggtcgaggt cgcgggaatc atcgcgcgcc gttggcgatc caatgcgcga
      721 tggcgcgcgc atcggcctcg ctcagttgcg gctgcgccgg catcgggatc gcgccccagg
      781 tgccttggcc gccgttgcgt atcctggcgg cgagaaagcc cgtgaggtcg gtgctgccga
      841 cgtgcttttg cgccacctcg cgaaaagccg gcccgacgat cttctggtcg atgccgtggc
      901 atccggtgca accgttggcc ttgatcagcg cctgcacgtc gacgccggct ggcgtcgcgg
      961 cgatcgacgc ggcctgcggc gtcgcggcca cggcgggcgc cgggccggcc gcggaaggcg
     1021 ctgcggcatc cgcgccgccg ccggccaacg ccgcgatccg cgcggtgtcg gtgccgcgct
     1081 ccgggccgaa cggccgcacc tggtcggcga tgttgccgtt ggaattcatc gccgcggccg
     1141 gaatcgacga ctcgatccgg ccctcgaccg gacagtcgcg catgcacagc ggattgctga
     1201 cgtcgccctt gccgcgcgtc tgccacatgc tcttgaagaa gacatggccg tagcggttgg
     1261 gcaggcgctt ctcgacctcg gcgatgttgg cgtcggagag cgtgaagtcg gccggaacga
     1321 tgtcgccgag gttgagaatg taggcgagca cgccgtacac ctcgtcggtc ttgagcgact
     1381 tcggcgcgtt ccacggcatc gcgcggttga cgtagtccca gatcgacgac aggtgcgaga
     1441 tcttcatcag cgtcgtgcgg tgcggatagc ccggatgcga aagaaaccag gtgtggccgc
     1501 tttcgatgtc cttcttcgac gtcccgccga cgatcggcgt gaagacctga ttcgactcgg
     1561 cgaactcgcc gtggcacgag gcgcacttcg cttcccagac ggtggtgccg aggtcgacgc
     1621 tgcccgcacc cttgggcagg ccctggaagt cgccgcgcac gtcgatgttc caggccgcga
     1681 cctcggcagg cgtcgccgcc cggccgatgc cggcccaggg caccgacgcc ggcgcggccg
     1741 gcgcgcgtgg cgcggcggcg accgcgagcg gcagcagcgc cagcgcaagg aacgatcgaa
     1801 cgaggagcgg acgtttcatc gcgacggcac cgttcaatac gcctggacgt tgctcacgtc
     1861 gcccgtctcg ctgacgcgcc acgactggat cgcgttgttg tgatagatcg agcgcgagcc
     1921 gcgcacggcg cgcagctggt tgatcttcgg ctgcacgtag ccggtgtcgt cgacggcgcg
     1981 cgattgcagg atcgccggcg agccgtccca gacccagtcg atgttgaagc gggtcagtgc
     2041 cttgccgagg accgggccct cgagccgcgc cgtgcgccag ttgcggccgc cgtcggcact
     2101 cacgtcgacg cgcgtcacct tgccgcggcc cgaccaggcc aggccggtga cgttgtagta
     2161 gcccttgtcg agcagcgtct gcgcgcccga cggcgtggtg atgaccgact tgcactcctg
     2221 gatcgaggtg aactgccgca acatgccgtc gggcatcagg tcgacgtagt ggctctcctc
     2281 gtccttggtg aagaacggct ggtcgccgac cttgatgcgg cgcaggtact tcacccagct
     2341 cacgccctgg atgccgggcg cgacgaggcg cagcgggtag ccgttctcgg gtcgcagcat
     2401 ctcgccgttc atgccccagg cgacgaggat gtcgtcgagc gcattggcca tcggcagcgt
     2461 gcgcgtcatc gacgagccgt cggcgccttc ggcgagcacg tagcggccgc gcttcgtgtc
     2521 ggcgccgcac atctcgagca aggtcgagag cctgacgccg gtgtattccg agcacgacag
     2581 catgccgtgc gtgtactgca cgctcggcac cgccgaattg ccccactcca tcgcgctgtt
     2641 ggcgccgcac tcgatgaagt ggatgcgcga gacctgcggc aggcgcatca ggtcgtccat
     2701 cgtgaagacg cgcgcctcct ccaccaggcc gttgatcatg aggcgatgct tcgacggatc
     2761 gatgtcccac cagccctggt gatgccgctc gaaatgcagg ccgctcggcg tgatgatgcc
     2821 gaacagcgac tgcagcgggc agaacgacac cgatgcctgc gccacctggg tgagcccggg
     2881 gctcggccgg cgctggatgt tcttttccca cttcgagggc gagccgtagc cggtgctcgc
     2941 gaccggcagg ccgaggccct tcgagtgctc gggcagccga aggatgttcg ggtcgccctc
     3001 gccgatcggc ggctgcgccg caaccgcggc acccgcgccc atcgccagcg ccgcgcccat
     3061 gaagcggcgg cgcgcccggc gcgcccgctc gagctcgtcg gcatcgagat ggttctcggg
     3121 cgccttgcgg atccgccccg gcatcgccgg caggttcatc gtctttcggg cctagcgcaa
     3181 tcgctcgatc aggccgtgca cgcccgtgcg caggccggtc ttctcgtcga gctcggacga
     3241 gacgcgcacg cagacggcgc ggcagacgtc ggtgagggtc gaatcggcga cgcggtagaa
     3301 cacctgcgcg ccgtcgcgtc gccgccgcag cgcgcccagg tggaacatca tgttgaggtg
     3361 gcgggacgcg ttgctttgcg tgagccccgt ctcggcgacg atctcgccca ccgttcgctc
     3421 gccctggcag acggcatgca ggatgcgaag ccgggccggc tcggcgagga cgaggaaata
     3481 acgggccacc gattcgaaca cctcgcccat ctcgtcggtc gcggccgtgg cgccgccggt
     3541 gggctcggtg acggccttcc cggttgcgcg ggtcggctcg ccaccggctg cggccgcagc
     3601 cttgccggcc ctggcggccc cgcgcgactt cctggcatcg atcgcttgca gcatccccgt
     3661 taatctatgc gcctatcctc atatgcgcat gggtagttgc ccgcgcttgg catgccgatg
     3721 gcgcccgtcg tctaatcgcg gcatggaagc cgtgcgtccc ctgcccactg ccctcgaggc
     3781 gcggcccggt ggcatcgaga tcgtcctgtt ctcccaggcc gactgcgagt tctgcgccga
     3841 gatccgcgat cactacctgc ggccgctgat ggccgagcgc cggcccgcga tcgccgtggc
     3901 cgagtcgcgc atcgacgccg acctgccgat acgcgactgg cagggacggt cgacgacgca
     3961 gcgcctcttc tccgccggcc agggcgtccg cttcgcaccg acggtgatgt tcttcgatgc
     4021 gtcggggcgc tcgctcgcgc ccccgatcgt cggcctgtcg accgacttct tcggcagcta
     4081 tctcgatcag cggatcgccg tggcgctggc cgctgcgcgc cggtccgaca atcgccccta
     4141 gacccatttt cgaggagaga cccgatgcgt cccacttccc gccgcctctt cgccttcggc
     4201 accggcctcg ccgccgcggc cgccgccctt ccctcggcgc gcgccgcggc tgccggagag
     4261 ccggggcgca acaaggtcgt cttccaggtg agcgatggcg atccgtcgaa gtggaatctc
     4321 accctcaaca acgtacgcaa cttccagacc gagcttcccg acgacgaaag cgacatcgag
     4381 atcgtcgcct acggcccggg catcgacatg ctgaagggcg gctcatcggc ggcgcaccga
     4441 atcgccgaga cgctcaagca cggcgtcaag gtggtcgcgt gcgagaactc catgcgcggc
     4501 cagaagctcg tctacgccga catgctcccc gacatcggct ttgtgccggc cggcgtcgtc
     4561 gagctcgtgc gcaagcaaca gcagggctac gcctacatcc gggcctgacc ggccggcgcg
     4621 cgccgccggc ttggcccgac cggggcgcag ccggcccacc gcgccgcgtc acccgaaggc
     4681 ccgcgtgcgt cggtgtcgcc tggccacgcc gccgggccgc gccgacccgg cgtccccgcc
     4741 ggcgtttccc ggccgagtcc cgtacagttg cgggccgcga ccgcgagacg acacgccttg
     4801 accgaagccg attttcaaca cacgaccgcg ctggtcctgg cactggcctt cgtcatcggc
     4861 gtcctgttcg gcgcgatcgg ccagcgcacg cgcttttgca cgatgggcgc catcagcgac
     4921 atggcggtga tgggcgatcc ggcacgactg cggatgtggc tggtggccgc cggcgtcgcc
     4981 atcgtcggct tcaacgcact cgccggcctc ggcctggtcc gcgccgccga cagcatctac
     5041 gcggcgccgc gcctgctctg gttgtccgcg ctcgtcggcg gagccatgtt cggcttcggc
     5101 atggtgctgg cgtcgggctg caccagcaag aacctgatgc gggccggttc cggcaacctg
     5161 aagtcggtcg tcgtgctgct cgtcgtcgcg ttcgccggct tcgccacgct gaagggtgcg
     5221 accgcgatcg tccgggtcga atggctcgac agcgtccacg tcgagctcgc cggcggccag
     5281 gacctgccgc acatcctcga gcggctcacc ggcgtcgccg cccgcccgct cgccgtgggt
     5341 ctcggcctgg ccctcggcgc ggcactgctg ctggcgggca tcgtgccgcg cgagacacgc
     5401 acgtccgacc tcttcgtggg cggcatcggc atcggcgtgc tgatcgttgc cgcgtggtac
     5461 gtctcgggcg tgatcggctt cgtcgccgaa aacccgaa
//
LOCUS       DASRWS010000291         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3573855, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000291 DASRWS010000000
VERSION     DASRWS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573855"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFF72_05420"
     CDS             <1..849
                     /locus_tag="VFF72_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZV92635.1"
                     /translation="AQGGEFGFVVFQTAAQAGVVDAPTSSLLVAAVAISMLLTPLFLV
                     VADRWWIPRIAGSRPGAEVEELHEPQDAPVIIAGFGRYGQIVGRMLYANGVKPTVLDH
                     DAEAIEALRKFGWPVFYGDATRLDLMRTAGADNARVLVLAIDDTQQSVECAKMVRENF
                     PDLTIVARARNVQHFYELFELGVTMIERETLDSALMTARGALEQLGWQPHHARNLALR
                     FRRHNVAQLAALAPHRKDEARLIAAAKQGRQQLEELFAIERRQAEERQSRAGWSPVQQ
                     EPPTPS"
     gene            978..1409
                     /locus_tag="VFF72_05425"
     CDS             978..1409
                     /locus_tag="VFF72_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HZV92636.1"
                     /translation="MFDPSPDQVRDFFVEAKDKRGRGAPLTPIETIAADWIAEHPEYD
                     ADLADAAKARAAVYDAGSGRENPFLHLSMHLSISEQVSIDQPRGIRQAFERLAARRGS
                     AHEAQHEVMECLGRMLWESQRSGRPPDAEAYVDCVRRRATR"
     gene            complement(1498..2070)
                     /locus_tag="VFF72_05430"
     CDS             complement(1498..2070)
                     /locus_tag="VFF72_05430"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HZV92637.1"
                     /translation="MELHGERLIPASRDATWAALNDPDILKACIAGCESLERTGDDAY
                     AAVVAMKVGPVSARFKGNLKMTQVDAPNGYTIAFDGQGGVAGFGKGSADVRLAAEGDA
                     TTRLVYDARATVGGKMAQIGSRLIDAAAAKITEDFFRAFEARLQARLPASVAAAPPVV
                     APPPMREPAGALGWVLSAIVVLVVVYLVFR"
     gene            complement(2084..3274)
                     /locus_tag="VFF72_05435"
     CDS             complement(2084..3274)
                     /locus_tag="VFF72_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZV92638.1"
                     /translation="MFADNVTHFARILRAAGMPVGPDRVLAALDALSVVGLGRRDDVH
                     AALSAVMIDRHEQQEIFDVAFAAFWRDPRLLEQMMAQMLPKVHGRADRDRRSNRLAEA
                     LATPRVEPKGRHADAGKDDEVDIETTFTFSDRERLQGTDFESMTAAEFELAKRIAEEL
                     PLPFAPVRRRRHEASARGHLDLRSTLLAMARQPATLVPRYSEPRSELPAIVVLLDISG
                     SMDRYARLMLHYVHGLTRRHLKVHTLTFGTRLTNITRALRNRDPDVALAMAASQVQDW
                     KGGTRIASCLDDFNRHWARRLLGANAAVLLVTDGLDTDEHGDLTRAARQLRLFAHQIV
                     WLNPLLRFDRFEPRAAGIRAILPNVDRFLPVHNLASLAELATALAPRRVAPRAAPQAS
                     RLAA"
     gene            complement(3264..>3900)
                     /locus_tag="VFF72_05440"
     CDS             complement(3264..>3900)
                     /locus_tag="VFF72_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZV92639.1"
                     /translation="HAVGDAAERQRLSHSLYSRDMLIERPLLQALTQDASPVLLIDEL
                     DRADEPFDAFLLEVLAENQLTIPELGTLKAKPGCEPIVVITSNRTREIHDALKRRCLY
                     HWVDYPSVERELEIVRIRAPGASDRLYAEVVEFVQRMRTVDLFKAPGVAEAIDWTNAL
                     VALNTMSLDPASVQDTLGVLLKHRDDIVKMQGGDVTRFLAELRETGRAGVR"
BASE COUNT          588 a         1348 c         1402 g          562 t
ORIGIN      
        1 gcgcaaggcg gcgagttcgg cttcgtcgtc ttccagacag cggcgcaggc tggcgtcgtc
       61 gacgcaccga cgtcttccct gctggttgcg gcggtggcga tctcgatgct gctgacgccg
      121 ctctttctcg tcgtcgccga ccgctggtgg attccgcgca tcgccggctc gcggccgggt
      181 gccgaggtcg aggagctgca cgagccgcag gacgcgccgg tgatcatcgc cggcttcggc
      241 cgctacggcc agatcgtcgg gcgcatgctc tatgccaacg gcgtcaagcc gacggtgctc
      301 gaccacgatg ccgaggcgat cgaggcgctg cgcaagttcg gctggccggt gttctacggc
      361 gacgcgacac gactcgacct gatgcgcacc gccggcgccg acaatgcccg ggtgctggtg
      421 ctcgccatcg acgacacgca gcaaagcgtc gagtgcgcga agatggttcg cgagaatttc
      481 cccgacctga cgatcgtcgc ccgggcccgc aacgtccagc acttctacga gctcttcgag
      541 ctcggcgtga cgatgatcga gcgcgagacg ctcgactcgg cgctcatgac ggcgcgcggc
      601 gcgctcgagc agctcggctg gcagccgcac cacgcgagaa acctggcgct gcgctttcgc
      661 cgccacaacg tcgcccagct ggcggccctc gcgccgcacc gcaaggacga agcccgcctc
      721 atcgccgccg ccaagcaggg ccgccagcag ctcgaggagc tgttcgcgat cgagcgccgc
      781 caggccgagg aacgccagtc gcgcgccggc tggagccctg tccagcagga gccgccgacg
      841 ccgtcgtgaa ggcgccggtc gatgcgccgt ggcgccggcg ggtcgatcat tgaccactct
      901 tggcgttgtc cgcatcgaac gaaaggactc gtcgccagct ctccggctgc gttcaggtgc
      961 gcatcgacaa tcgccctgtg ttcgacccca gccccgacca ggttcgcgat ttcttcgtcg
     1021 aggcgaagga caagcgcggg cgcggcgcgc cgctgactcc gatcgagacg atcgccgccg
     1081 actggatcgc cgagcatccc gagtacgacg ccgacctcgc cgacgcggcg aaggcgcgcg
     1141 ccgccgtcta cgacgccggg tccggccgcg agaacccatt cctgcacctg tcgatgcacc
     1201 tgtcgatcag cgagcaggta tcgatcgacc agccgcgcgg catccgccag gcgttcgaac
     1261 ggctggcggc ccggcggggc tcggcgcacg aggcccagca cgaggtgatg gaatgcctgg
     1321 gccgcatgct ctgggagtcg cagcgaagcg gccggcctcc cgacgccgag gcctacgtcg
     1381 actgcgtccg ccgccgcgcg acgcgctgag cgcgcctggc ctcacgcgtc accgacgctg
     1441 cccgaccgcc gcatcggcgg tcaagacgtg gccacgaagg aacgcggccg gtcgacgtca
     1501 gcgaaagacg agatacacga cgaccaggac gacgatcgcc gacagcaccc agccgagcgc
     1561 accggcgggc tcgcgcatcg gcggcggcgc cacgaccggc ggcgccgcag ccaccgacgc
     1621 cggcaggcgc gcctgcagac gagcctcgaa ggcacggaag aagtcttccg tgatcttcgc
     1681 cgccgcagcg tcgatgagac gcgagccgat ctgcgccatc ttgccgccga cggtggcgcg
     1741 agcgtcgtag acgagccgcg tcgtcgcgtc gccctcggcg gcgagccgca cgtcggcgga
     1801 gcccttgccg aaaccggcga cgccgccctg gccgtcgaag gcgatcgtgt agccgttcgg
     1861 cgcgtcgacc tgggtcatct tcaggttgcc cttgaagcgg gcactgaccg gaccgacctt
     1921 catcgccacg acggcggcgt acgcgtcgtc gccggtgcgc tcgagcgatt cgcagccggc
     1981 gatgcaggcc ttgaggatgt cggggtcgtt gagcgcggcc caggtcgcgt cgcgcgacgc
     2041 cgggatcagt ctttcaccgt gcagttccat gacgccgatt ctgctacgcg gcgaggcggc
     2101 tcgcctgagg cgccgctcgt ggcgcgacgc ggcgcggggc gagcgcggtc gcgagctcgg
     2161 ccaggctggc caggttgtgc accggcagga agcggtcgac gttgggcagg atggcgcgga
     2221 tgccggccgc gcgcggctcg aaccggtcga agcgcagcag cgggttgagc cagacgatct
     2281 ggtgggcgaa gaggcgcagc tggcgggcgg cacgcgtcag gtcgccgtgc tcgtcggtat
     2341 cgaggccgtc ggtgaccagc agcaccgcgg cgttggcacc gagcaggcgc cgcgcccagt
     2401 ggcggttgaa gtcgtcgagg cacgacgcga tgcgggtgcc gcccttccag tcctgcacct
     2461 gcgacgccgc catcgccagc gccacgtcgg ggtcgcggtt gcgaagcgcc cgcgtgatgt
     2521 tggtcaggcg cgtgccgaag gtcagggtgt gcaccttgag gtgtcttcgc gtcaggccgt
     2581 gcacgtagtg cagcatgagg cgggcgtagc gatccatcga acccgagatg tcgagcagga
     2641 cgacgatcgc cggcagctcg ctgcgcggct cgctgtagcg cggcaccagc gtcgccggct
     2701 ggcgcgccat cgccagcagg gtcgagcgca gatcgagatg gccgcgcgca ctggcctcgt
     2761 gacggcggcg tcgcaccggc gcgaacggca gcggcagctc ctcggcgatg cgcttggcga
     2821 gctcgaactc ggccgccgtc atcgactcga aatccgtgcc ctgcaggcgt tcgcgatccg
     2881 agaaggtgaa ggtcgtctcg atgtcgacct cgtcgtcctt cccggcgtcg gcgtgtcggc
     2941 ccttcggctc gacgcgcggc gtcgccaggg cctcggccag ccgattcgag cgccggtcgc
     3001 gatcggcgcg gccatgcacc ttgggcagca tctgcgccat catctgttcg agcaggcgcg
     3061 gatcgcgcca gaaggcggcg aaggcgacgt cgaagatctc ctgctgctcg tggcggtcga
     3121 tcatcaccgc cgacagcgcg gcgtggacat cgtcgcggcg gccgaggccg acgaccgaga
     3181 gcgcgtcgag ggcggcgagc acgcggtcgg gaccgaccgg cattcccgcg gcgcgaagga
     3241 tgcgggcgaa gtgggtgacg ttgtcagcga acaccggcgc gacccgtctc gcgcagctcg
     3301 gccagaaaac gcgtcacgtc gccgccctgc atcttgacga tgtcgtcgcg atgcttgagc
     3361 agcacgccga gcgtgtcctg cacgctggcc gggtccaggc tcatggtgtt gagggcgacc
     3421 agcgcattgg tccagtcgat ggcctcggcg acaccgggtg ccttgaacag gtcgacggtg
     3481 cgcatgcgct gcacgaactc gacgacctcg gcatagaggc gatccgaagc acccggcgcg
     3541 cggatgcgca cgatctccag ctcgcgctcg accgacgggt agtcgaccca gtgatagagg
     3601 cagcggcgct tcagcgcgtc gtggatctcg cgcgtgcggt tgctggtgat gacgacgatc
     3661 ggctcgcatc ccggcttcgc cttcagcgtg cccagctcgg gaatggtgag ctggttctcg
     3721 gccagcacct cgagcaggaa cgcatcgaat ggttcgtcgg cacggtcgag ctcgtcgatc
     3781 agcagcaccg gcgaggcgtc ctgcgtcaac gcctgcagca gcggtcgctc gatcagcatg
     3841 tcgcgcgagt agaggctgtg cgacaggcgc tggcgctcgg ccgcgtcgcc gacggcgtgc
//
LOCUS       DASRWS010000292         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1835998, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000292 DASRWS010000000
VERSION     DASRWS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835998"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..284
                     /locus_tag="VFF72_05445"
     CDS             6..284
                     /locus_tag="VFF72_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HZV92640.1"
                     /translation="MATKKRWSQHVTDTSDALDLKDGVFRLTDPKKIARSLKASAEHS
                     DRRKSDPYRSAMSMLTFYINRAGSTLPKAQHKHLEDAKDELRKLFGRA"
     gene            467..646
                     /locus_tag="VFF72_05450"
     CDS             467..646
                     /locus_tag="VFF72_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HZV92641.1"
                     /translation="MSDDRTKTGQDRKLIALTEPYEVRDWCKTLGCSKEELERAVKAV
                     GHSADKVREFLRSGS"
     gene            711..989
                     /locus_tag="VFF72_05455"
     CDS             711..989
                     /locus_tag="VFF72_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92642.1"
                     /translation="MDALGLLQWPAMVVTLLAAWLVASKRRSRRRWGFATFILSNILW
                     IVWGWHTASYALIVLQVGLFFMNRRGEEQNSQAGSSGKPKTIGPSSGG"
     gene            1240..>2859
                     /locus_tag="VFF72_05460"
     CDS             1240..>2859
                     /locus_tag="VFF72_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HZV92643.1"
                     /translation="MSQNVAEFVWKRLSEWGLSRVYGYPGDGVGGLDVALEKARDFMQ
                     YVQVRHEEMAAFMASAHAKFTGQVGLCYATSGPGAIHLLTGLYDAKADHVPVVAIVGQ
                     QARSALGASYQQEVDLQNLFKDVASDYVMTAVVPEQVRTVIDRAVRIAQSKRAVTCVI
                     LPNDLQLLDYVDPPVAHGATHTGVGYPGVSTLPEPDALRRAADILNAGKKVAMLVGAG
                     ALHATDEVMAVADRLNAGVAKALLGKAVLPDDLAYVTGSLGLLGTKPSWDLMKECDTL
                     LMVGSAFPYSEFLPEPGKARGVQIDIDGANLSLRYPMEYSLAGDSRQTLAALLPLLQQ
                     NTNDDWRRGIEKNVAAWWKTLEERAMAAAKPINPQRIFWELSPLLPDDVIMTGDSGSV
                     ANWYARDIKIRRGMMASLSGGLASLGAATPYAVAAKMAYPHRPVIGFIGDGAMQMNGL
                     NVMITVGKYWKNWPDPRFIIMVLNNQDLNQVTWEERIQIGSGKTESTQSIPDFAYHTY
                     AELLGLKGIMVNDPERIGAAWDEALAADRPVV"
BASE COUNT          525 a          959 c          945 g          430 t
ORIGIN      
        1 cggcgatggc gaccaaaaag cgctggtccc aacacgtcac tgacaccagc gatgcgctcg
       61 atctgaagga cggggtcttc aggctgaccg atccgaagaa gatcgcccgc tcgctgaagg
      121 cttcggccga gcacagcgac cgcaggaaga gcgacccgta tcgctcggcc atgtcgatgc
      181 tcaccttcta catcaaccgc gccggcagca cgcttcccaa ggcccagcac aagcatctcg
      241 aagacgcgaa ggacgagttg cgaaagctgt tcgggcgggc gtgacggccg caatggccgc
      301 ggatcgccgc cgtcacgcga acgcgttttg cgcaaccgat cggggcgagc aggcacgcgg
      361 acggctctcg ccgatgagta cgcgagtacg tcgatccgaa gccggccacc cggcctcggg
      421 tacgccatat gccctcgcca tggaccgaat gacgaaaggt gctttgatga gcgacgacag
      481 gacgaagacc ggccaggacc gcaagctgat tgccctgacc gaaccctacg aagtacgaga
      541 ctggtgcaag acgctcggct gctcgaaaga ggaactcgag cgcgcggtca aggcggtcgg
      601 acactcggcc gacaaggtgc gcgagttcct ccggagcggc tcgtaaggcg gccgagggaa
      661 ctcgcgatcg cgacgactga acgcgaaccg gtcgcccgtc catcgatgcc atggacgcgc
      721 taggcctgct tcagtggccg gccatggtcg tcacgctcct ggccgcgtgg ctcgtcgcct
      781 cgaaacgccg gtctcgtcgc cgctggggat tcgccacgtt catcctcagc aacatcctgt
      841 ggatcgtctg gggctggcac acggcgagct acgccttgat cgtcctgcag gtcggcctct
      901 tcttcatgaa ccggcgcggc gaggaacaga attcgcaagc cggatcgtcc ggtaagccaa
      961 agacgatcgg cccttcgtcg ggcggttagc gatgcgtcga tcgatgcatc gccgaagcgc
     1021 aaggtgacga ccgcgaggca ccgtcggcga cgccggagcg tcccgggtac cgaagcgtgc
     1081 gcgatctcga ctctctcgcc tgcctgctga agcggcaagc gtgaatgcgg cgccagcacc
     1141 cgcgcttcgg cgccgccttc atgatccggc gacgccgccg gcggcgctga accccggcat
     1201 cggaattgcc atgactcgag gcgcagccaa ggagtcaccg tgtcgcaaaa cgtcgccgag
     1261 ttcgtctgga agcgcctgtc cgaatggggg ctctcgcggg tctacggcta tccgggcgac
     1321 ggcgtcggtg gtctcgacgt ggcgctcgaa aaggccaggg acttcatgca gtacgtgcag
     1381 gtccggcacg aggaaatggc cgccttcatg gcttcggcgc acgcgaagtt caccggccag
     1441 gtcggccttt gctacgcgac gtcgggaccc ggtgccatcc atctgctgac gggcctctac
     1501 gacgcgaagg ccgaccacgt cccggtggtc gcgatcgtcg gccagcaggc gcgcagcgcg
     1561 ctcggcgcca gctaccagca ggaggtcgac ctgcaaaacc tcttcaagga cgtggcgtcc
     1621 gactacgtca tgaccgccgt ggtgcccgag caggtgcgaa cggtcatcga ccgcgccgtt
     1681 cgcatcgccc agagcaagcg cgcggtgacc tgcgtgatcc tgccgaacga cctgcaactg
     1741 ctcgactacg tcgatccgcc ggtggcgcac ggcgcgacgc acaccggggt cggctatccc
     1801 ggcgtctcga cgctgccgga accagacgcg ctccgacgcg cggccgacat cctcaacgcc
     1861 ggcaagaaag tcgcgatgct tgtcggcgcc ggcgcgttgc acgcgaccga cgaggtcatg
     1921 gcggtcgccg atcgcctcaa cgccggcgtc gccaaggcgc tcctcggcaa ggccgtgctt
     1981 ccggacgatc tcgcctacgt gaccggctcg ctcggcctgc tgggcaccaa gccgagctgg
     2041 gacctgatga aggagtgcga cacgctgctc atggtcgggt cggcgtttcc gtacagcgaa
     2101 ttcctgcccg agcccggcaa ggcccgcggc gtgcagatcg acatcgacgg cgccaatctc
     2161 agcctgcgct acccgatgga atattcgctt gccggcgaca gcaggcagac gctggcggcg
     2221 ttgctgccgc tgctccagca gaacacgaac gacgactggc gacgcggcat cgagaagaac
     2281 gtcgccgcct ggtggaagac gctcgaggaa cgggcgatgg cggcggccaa gccgatcaac
     2341 ccgcagcgca tcttctggga gctctcgccg ctgctgcccg acgacgtgat catgaccggc
     2401 gacagcggct cggtcgccaa ctggtacgcg cgcgacatca agatccggcg cggcatgatg
     2461 gcttcgctct cgggcggcct cgcctcgctc ggcgccgcga cgccgtacgc ggtggcggcg
     2521 aagatggcct acccgcaccg gccggtgatc ggcttcatcg gcgacggcgc gatgcagatg
     2581 aacggcctca acgtgatgat cacggtcggc aagtactgga agaactggcc cgatccgcgc
     2641 ttcatcatca tggtcctgaa caaccaggac ctgaaccagg tcacctggga ggaacgcatc
     2701 cagatcggct ccggcaagac ggaatccact cagagcatcc ccgatttcgc ctaccacacc
     2761 tatgccgagc tgctcgggct gaagggcatc atggtcaatg atcccgagcg gatcggcgcg
     2821 gcctgggacg aggcgctggc ggcggatcgg ccggtggtc
//
LOCUS       DASRWS010000293         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_118030, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000293 DASRWS010000000
VERSION     DASRWS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118030"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFF72_05465"
     CDS             complement(<1..507)
                     /locus_tag="VFF72_05465"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HZV92644.1"
                     /translation="MAFSLDTAIAAHRFGIGEADLGVVGGDPRGWLTAQIGPADPARG
                     EGLLDTAQALAFVAAERERRAMAKNPPPGMTAAQVLGGYYREVIVADSRSRLATATLT
                     GRPFMERTQWFWTNHFTVSLAKGSTRGLVGAFERDAIRPHLEGRFETMLVASTTHPAM
                     LRYLDNSQS"
     gene            complement(632..1981)
                     /locus_tag="VFF72_05470"
     CDS             complement(632..1981)
                     /locus_tag="VFF72_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZV92645.1"
                     /translation="MDFASPARHAFRLAAAHRLAALGVAAGALLAPGTAGAQSLLELY
                     EAAHAYDAAYLSARSLAESAPYRVEQTRALMRPNVTVSAGASRDTVKPPAEYGYDSNT
                     LQATIGVRQPLFNRANSSSIAQADRSLESSLADLDSAEQDLMLRVAQAYFDVLAAQDT
                     LTTTRANKAAIAEQLASAKRNFEVGTATITDTREAQARFDLATAQEVTADNDLVTRRI
                     ALDQLVGRDGVVPRQLRVPVVLPLPTTSLEQLVADAALDHPAVRRARVAYDIAALETE
                     KARAQGLPTVDAVASAGGGRSTIAPGFTGPALNTSIGVQINMPLFAGFAIQNRLKETL
                     LLEERARNDFDAAQRGVVQATRQAYYTLQSSAAQVKALEAAEASSQLALEATQVGYRV
                     GVRVNVDVLNAQSQLYQTRRDLARARYDVLLNTLRLDQQAGRLTPGDVLAINNLLAP"
     gene            complement(2175..2867)
                     /locus_tag="VFF72_05475"
     CDS             complement(2175..2867)
                     /locus_tag="VFF72_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZV92646.1"
                     /translation="MNIEQARFNMIEQQIRTWEVLDDSVLSLLSVVKREDFVPAAYRA
                     MAFVDTELPLPHGQSMLAPRVEARLLQELAVQRHERVLEIGSGSGYMAALLAHKARHV
                     TTLEIVPDLAQFAADNLRRAWIANATVLEADASKAPPIDGPFDVIVLSGSVAEVPPAL
                     LGQLKPAGRLVAIVGQLPVMRAVLLTRAAAETGPDAPRDVVPGGGHSTVELFDTVAPR
                     LVGFGEPSRFTF"
     gene            2996..>3710
                     /locus_tag="VFF72_05480"
     CDS             2996..>3710
                     /locus_tag="VFF72_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZV92647.1"
                     /translation="MTRSLPFRNPYRHDFVRVGVGVPRLRVADPDYNAKETVALLERA
                     CAEGVVVVVFPELGLAGYTCDDLFHQQAVLDAAEKALSSVLEATRRLPLLAAVGLPLR
                     VEQKLFNCAAIVHGGRVLGVVPKTYLPNYGEFYEARQFAAAEAALSSEIEVLGQTVPF
                     GNDLVFRAADLPLLALHAEICEDLWVPIPPSSYAALAGATVLLNLSASNVTVGKAGYR
                     RDLVSGQSARCLAAYLYSAS"
BASE COUNT          513 a         1230 c         1383 g          584 t
ORIGIN      
        1 cgactgcgaa ttgtcgaggt agcgcagcat cgccgggtgc gtggtcgagg cgacgagcat
       61 cgtctcgaag cgtccttcga ggtgcgggcg gatcgcatcg cgctcgaagg cgccgacgag
      121 gccgcgcgtg cttcccttgg cgagcgacac cgtgaagtgg ttggtccaga accactgcgt
      181 ccgttccatg aacgggcgtc cggtcagcgt ggccgtcgcc aggcgcgagc gcgagtcggc
      241 gacgatcact tcgcggtagt agccgccgag cacctgtgca gcagtcatcc cgggcggggg
      301 atttttcgcc atcgcgcggc gctcgcgctc ggcggcgacg aaggccagcg cctgtgccgt
      361 gtcgagcagg ccttcgccgc gcgccggatc ggccggcccg atctgcgccg tcagccaacc
      421 ccgcgggtcg ccgccgacga cgccgaggtc ggcctcgccg atgccgaacc ggtgcgctgc
      481 aatcgcggtg tcgagagaga aggccatgcg cgttcctgct tgacggcatt gtcttgccgg
      541 cagagtcaac ggcgcatgtg cgcccggtca agaaatgtgc agtcgggcac ggcggccgtt
      601 cggcggcgga gcgccagccg cccgcagcct ttcagggcgc caggaggttg ttgatcgcca
      661 gcacgtcgcc cggcgtcagg cgaccggcct gctggtcgag gcgcaaagtg ttgaggagaa
      721 cgtcgtagcg ggcgcgggcg aggtcgcggc gcgtctggta gagctggctc tgcgcattga
      781 ggacatcgac gttgacgcgc acgccgaccc ggtagccgac ctgcgtcgct tccagcgcga
      841 gctggctcga tgcctcggcc gcttccagcg ccttgacctg ggcggcgctc gactgcaggg
      901 tgtagtaggc ctgccgggtc gcctggacga caccgcgttg cgcggcgtcg aaatcgttgc
      961 gggcgcgctc ctcgagaagc agcgtctcct tgagccggtt ctggatcgcg aagccggcaa
     1021 agagcggcat gttgatctgc acgccgatgc tggtgttcag cgccggaccg gtgaagcccg
     1081 gcgcgatcgt gctgcgaccg ccgccggcgc tcgccaccgc gtcgacggtc ggcagcccct
     1141 gggcccgggc cttctccgtt tcgagcgcgg cgatgtcgta ggcgacgcgg gctcggcgca
     1201 cggcgggatg atcgagcgcc gcgtcggcga cgagctgctc gaggctcgtc gtcggcagtg
     1261 gcagaacgac gggcacccgc aactgccgcg gcacgacgcc gtcgcgaccg acgagctggt
     1321 cgagagcgat gcggcgcgtg acgaggtcgt tgtcggcagt cacctcctgc gccgtggcga
     1381 ggtcgaagcg ggcctgcgcc tcgcgcgtgt cggtgatcgt cgccgtgccg acctcgaaat
     1441 tgcgcttcgc cgatgccagc tgttcggcga tggccgcctt gttcgcgcgg gtcgtcgtca
     1501 aggtgtcctg ggcggcgaga acgtcgaaat aggcctgggc gacgcgcagc atcaggtcct
     1561 gctcggccga gtcgagatcg gcgagcgacg attcgagcga gcggtcggcc tgggcgatgg
     1621 acgacgagtt ggcgcgattg aaaagcggtt gccggacccc gatcgtcgcc tgcagcgtgt
     1681 tgctgtcgta gccgtattcg gccggcggct tgacggtgtc gcgcgaggcg ccggcgctga
     1741 cggtgacgtt cggccgcatc agggcgcgcg tctgctcgac ccggtagggc gccgactcgg
     1801 ccagcgagcg cgccgagaga taggccgcgt cgtaggcgtg cgccgcctcg tagagctcga
     1861 ggagcgactg cgcgccggcc gtgccggggg ccagaagggc gccagcggcg acgcccaggg
     1921 cagcgaggcg atgggctgcg gcgaggcgaa aggcgtggcg ggcaggcgag gcgaagtcca
     1981 tggatcgcac atttcttgtt cccggccggt gaacatggct ccctcggcgc gggccgggcc
     2041 gccgcgtcgc ttgcgcggtg ttataggcga cgcgaggccg gccggcgagg ttcttgtgac
     2101 ggattgcgcg cagcggccgc cgaagcggcg atggccgtga caggcggctt cccggcggcc
     2161 cgccgtttgc ggcgtcagaa ggtgaagcgg ctgggctcgc cgaagccgac cagcctcggc
     2221 gcgacggtat cgaacagctc gacggtggag tggccgcccc cggggacgac gtcgcgcggg
     2281 gcgtcgggcc ccgtctcggc ggcggcgcgg gtgagcagca ccgcccgcat caccggcagc
     2341 tggccgacga tcgcgaccag ccggccggcc ggcttcagtt gtccgagcaa cgccggcggc
     2401 acctccgcca ccgatccgga caggacgatg acatcgaacg gcccgtcgat cggcggcgcc
     2461 ttcgacgcgt cggcctcgag aaccgtcgcg ttggcgatcc aggcccgccg caggttgtcg
     2521 gcggcgaatt gcgccaggtc gggaacgatc tcgagcgtcg tcacatgccg ggccttgtgc
     2581 gcgagaagcg cagccatgta gcccgacccg gagccgatct cgaggacgcg ctcgtggcgt
     2641 tgcacggcga gctcctgcag cagccgggcc tcgacccgcg gcgccagcat cgactggccg
     2701 tgcggcagcg gcagctcggt gtcgacgaag gccatcgccc gataggcggc cggaacgaag
     2761 tcttcgcgct tgacgaccga cagcagcgac aggacgctgt cgtcgagcac ctcccaggtg
     2821 cggatctgct gctcgatcat gttgaatcgg gcttgttcga tgttcatggc ggtctggcgc
     2881 aaggattcgg cgaaaccggc aggataacgg ggcggccgcc cgacgtcgtg gcacgccggc
     2941 acggcgagcg ccggcgcatg acatcatcgt cgtcgtgaca ggaggctttt cacgggtgac
     3001 gcgctcgctg ccgtttcgca acccctaccg ccacgacttc gttcgcgtcg gtgtcggcgt
     3061 cccgcgattg cgcgtcgccg atccggacta caacgcgaag gaaacggtcg cgcttctcga
     3121 gcgcgcctgt gccgagggcg tcgtcgtcgt cgtcttcccc gagctgggcc tggccggcta
     3181 cacctgcgac gacctcttcc atcagcaggc ggtgctcgat gccgccgaaa aggcgctttc
     3241 gtcggtgctc gaggcgacgc ggcgcctgcc gctgctggcc gcggtgggcc tgccgcttcg
     3301 cgtcgagcaa aagctcttca attgcgcggc gatcgtccac ggcggccggg tcctcggcgt
     3361 cgttccgaag acctaccttc ccaactacgg agagttctac gaggcgcgcc agttcgccgc
     3421 ggccgaggcg gcgctgtcca gcgagatcga ggtgctgggc cagaccgtgc ctttcggcaa
     3481 cgacctcgtg ttccgggccg ccgacctgcc gttgctggcc ctgcatgccg agatctgcga
     3541 agacctctgg gtgccgatcc cgccgtcgtc gtacgcggcg ctggccggcg cgacggtgct
     3601 gttgaacctg tcggcctcga acgtgacggt cgggaaggct ggctatcggc gggatctggt
     3661 gtctgggcag tcggcgcgct gcctggcggc gtatctgtac tccgcctcgg
//
LOCUS       DASRWS010000294         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2700616, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000294 DASRWS010000000
VERSION     DASRWS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700616"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFF72_05485"
     CDS             <1..602
                     /locus_tag="VFF72_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV92648.1"
                     /translation="VRAGLKQFFSDQVDLTVVAEAANGRDAIDIVRKGDVDVILMDLS
                     MPDQSGVDALAAIKARAPDLPVLILSGFPEEHYATTLLRQGASGYLNKDCDPEEIVKA
                     IRTVYRGRKYITAGVAELLADGLGGGSADRPAHEQLSEREFQVFLRLAKGETIGHMAN
                     SMSLSVKTVSTYRTRVMEKMKLESNSDLTYYALKNGLIQ"
     gene            complement(642..1052)
                     /locus_tag="VFF72_05490"
     CDS             complement(642..1052)
                     /locus_tag="VFF72_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92649.1"
                     /translation="MTQLRAFIVEDSPVIRENLVAALEEMAPIDVVGTAEDEASAVSW
                     LALPGNPCDLVVVDIFLKSGSGLGVLKSATSAPSTPKLVVLSNYATPDMRRKCLELGA
                     DRVFDKSNEIDALILYCCRLADGGTGSSELDALA"
     gene            1246..2808
                     /locus_tag="VFF72_05495"
     CDS             1246..2808
                     /locus_tag="VFF72_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV92650.1"
                     /translation="MFVSASIFASSSLAADGDTRTTVVGSKRFTESYILGEIVTQTLR
                     AAGRPAVHKQGLGNTGILEQALADGSIDLYPEYTGTIVRELLKREGNPWLDELNRWLS
                     PRGFAVAVPFGFNDSYALAMSEAKARSLGISRLSDLASAKARSLRFGLSHEFLQRGDG
                     WPALQKAYAIEATPIGLDHGLAYEAITSGKVDVIDVYSTDAKLGRLGLRVLVDDRDFF
                     PKYDAVLLMRKGLDIAPLQKLRGSIDDKAMIAMNAQVELDGRSFADVARQFLVSRGIV
                     AASASASSAAAASRTGASAAAAAGAASALTARPGLLARIFAPDFARLAGEHLVLVFGS
                     LALAIAVGVPMGIAAWRWPACAGVLLGAVGVLQTVPSLALLAFLIALLGMIGVVPALV
                     ALFLYALLPIVRNTYAGLDSVTRSMGQAALSLGLTRPQALAFVQLPLATPTLLAGIKT
                     AAVINVGTATMAAFIGAGGFGERIVAGLAVNDNAVMLAGALPAAVLALLVQLAFDAAE
                     RWALPSRAAQRS"
     gene            2840..>3837
                     /gene="rmuC"
                     /locus_tag="VFF72_05500"
     CDS             2840..>3837
                     /gene="rmuC"
                     /locus_tag="VFF72_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZV92651.1"
                     /translation="MTFDWLMPALLAVLVGITLWIALRRPPVDADAQRQGRDLREEVA
                     RSAQGVRVEIGSMLGDFQRTLLTQQGDAARTQNERQTAALKDLAESLGRQLQALVESN
                     DRRMGEMRGSVEGRLAAIQVDNEKKLEQIRATVDEKLHATLEQRLGESFRQVAERLEQ
                     VHRGIGEMQKLASDVGSLNRVLTNVKTRGMFGEVQLAALLEQALTIEQYAVNVETVPG
                     SGARVEFAIKLPGRRDDNAPLWLPIDCKFPRDDYERLVEAQEAADRVGVETYSRAIEM
                     RLRKEALTIREKYVAPPHTTDFGILFLPTEGLYAEALRRPGLVESLQRDYKVMLTGP"
BASE COUNT          636 a         1274 c         1296 g          631 t
ORIGIN      
        1 ttgtccgggc aggcctcaag cagttctttt ccgaccaggt cgacctgacg gtcgtggccg
       61 aggccgcgaa cggcagggac gcgatcgaca tcgtgagaaa gggcgacgtc gacgtcatcc
      121 tcatggacct gtcgatgccc gaccagagcg gcgtcgacgc gctggcggcg atcaaggcgc
      181 gggcgcccga tcttccggtg ctcatccttt cgggctttcc cgaagagcac tatgcgacga
      241 cgctgcttcg ccagggtgcc agcggctacc tgaacaagga ttgcgacccc gaagagatcg
      301 tcaaggcgat ccgcacggtc tatcgcggcc gcaagtacat caccgccgga gtggccgagc
      361 ttcttgccga cggcctcggc ggcggcagcg ccgaccggcc ggcccacgaa cagctttccg
      421 agcgcgagtt ccaggtgttc ctgcgcctgg ccaagggcga gaccatcggc cacatggcca
      481 acagcatgtc gctcagcgtc aagacggtca gtacctatcg gacccgggtc atggagaaga
      541 tgaagctcga gtcgaacagc gatctcacct actacgccct gaagaacggg ctcatccagt
      601 aggaagcggg aacgccgccc agcgaaacgc acggcgcgtt cctaggccag tgcgtcgagc
      661 tcgctgcttc ccgttccgcc atcggcgagg cgacagcaat acaggatgag cgcgtcgatc
      721 tcgttggact tgtcgaagac acgatcggcg ccgagctcca gacacttgcg ccgcatgtcg
      781 ggggtcgcgt agttgctgag gacgacgagc ttgggcgtgc tcggcgccga agtcgccgac
      841 ttcagtacgc ccagccccga accgctcttg agaaaaatgt cgacgaccac gagatcgcac
      901 ggattgcccg gaagcgcgag ccagctcact gccgacgcct cgtcctctgc cgttccgacg
      961 acgtcgatcg gtgccatctc ctcgagcgcg gcgacgaggt tttccctgat cacggggctg
     1021 tcctcgacga tgaacgcgcg caactgggtc atggacgagc gtttctcatc ctgttccggc
     1081 acctcgcgcc gggaagcggg cgaaggggag tggacgacga cgagggacaa ggcgaaggca
     1141 cgctctgcgg gcgagaggga aggtcgaagt gtcgtccgca gtgtcggcaa actcctaggc
     1201 caaggggctg ttttgcgcgt aggagcagtc ctacgaatcg tcctgatgtt cgtgagcgcg
     1261 tcgatcttcg cctcgtcgtc gctcgcagcc gacggcgaca cacgcacgac ggtggtgggc
     1321 tcgaagcgat tcaccgagtc gtacatcctc ggcgagatcg tcacccagac gttgcgcgcc
     1381 gccggccgac ccgccgtgca caagcagggc ctcggcaata ccggcatcct cgagcaggct
     1441 cttgccgacg gctcgatcga cctctatccc gaatacaccg gcacgatcgt tcgggaattg
     1501 ctcaagcgcg aaggcaatcc ttggctcgac gagctcaatc ggtggttgag cccgcgcgga
     1561 ttcgccgtcg ccgttccgtt cggcttcaac gacagctatg cgctggcgat gagcgaagcc
     1621 aaggccaggt cgctcgggat ctcgcgcctc tcggatctcg cctcggccaa ggcgcgcagt
     1681 ctgcgcttcg gcctgtcgca cgagtttctc cagcgaggcg atggctggcc ggctcttcaa
     1741 aaggcctatg cgatcgaggc gacgccgatc ggcctcgatc acggcctggc gtacgaggcg
     1801 atcaccagcg gcaaggtcga cgtgatcgac gtctactcga ccgatgccaa gctcggccgg
     1861 ctcggcttgc gtgtcctggt cgacgatcgc gatttctttc cgaaatacga cgcggtcctg
     1921 ctgatgcgaa aggggctgga catcgcgccg ctgcaaaagc tgcgcggcag catcgacgac
     1981 aaggcgatga tcgcgatgaa cgcgcaggtc gagctcgacg gacgcagctt tgccgacgtg
     2041 gcccggcagt tcctggtgtc tcggggcatc gtcgcggcga gcgcaagcgc gagttcggct
     2101 gcggcggcgt cgcgaaccgg cgcatcggcc gcggccgcgg ctggcgccgc ctcggccttg
     2161 accgcacgtc ccggtctcct cgcccggatc ttcgcccccg acttcgcacg cctggccggc
     2221 gagcaccttg tccttgtctt cggctcactg gcgctggcca tcgccgtcgg ggtgccgatg
     2281 ggcatcgcgg cctggcgctg gccggcatgc gccggcgtgc tgctcggcgc cgtcggtgtg
     2341 ctgcagaccg tcccgtcgct cgcgctgctg gcgttcctga tcgccctgct cggcatgatc
     2401 ggcgtcgtcc cggcgttggt cgcgcttttc ctctacgcct tgttgccgat cgtgcgaaat
     2461 acctacgccg ggctcgacag cgtgacgcgc agcatgggcc aggcggcgct gtccctgggc
     2521 ctgacccggc cgcaggcgct cgccttcgtg caactgcccc tggccacacc gaccctgctg
     2581 gccggcatca agacagcggc cgtcatcaac gtcggcaccg cgacgatggc ggcgttcatc
     2641 ggtgccggcg gcttcggcga gcgaatcgtc gccgggctcg ctgtgaacga caacgccgtc
     2701 atgctggccg gtgcgctgcc cgccgcggtg ctggccctgc tggtccagct ggccttcgat
     2761 gccgccgagc gctgggcact gccgtccagg gcggcgcagc gctcctgagc gagcgcgtcg
     2821 gcgctgcgac aatcgggcga tgacgttcga ctggctgatg ccggcgcttc tcgccgttct
     2881 tgtcggcatc accttgtgga tcgcgttgcg ccgaccgccc gtcgatgccg acgcgcagcg
     2941 ccagggccgc gaccttcgcg aggaggtggc acgcagcgcc cagggcgtcc gcgtcgagat
     3001 cggctcgatg ctcggtgatt tccagcgcac cctgctgacc cagcaaggcg atgcggcgcg
     3061 cacccagaac gagcgccaga cggcggcgct gaaggatctg gccgaatcgc tcgggcgcca
     3121 gttgcaggca ctggtcgaga gcaacgacag gcgcatgggc gagatgcgcg gcagcgtcga
     3181 gggcaggctg gcggcgatcc aggtcgacaa cgagaaaaag ctcgagcaga ttcgcgcgac
     3241 ggtcgacgag aagctgcacg cgaccctgga gcagcggctg ggcgaaagct tccgccaggt
     3301 cgccgagcgc ctggaacagg tgcatcgcgg catcggcgag atgcaaaagc tcgccagcga
     3361 tgtcggctcg ctcaatcgcg tcctcacgaa tgtgaaaacg cgcgggatgt tcggcgaggt
     3421 gcagctcgcg gccctgctcg agcaggcgct gacgatcgag caatacgctg tgaacgtcga
     3481 aacggtgccg gggtcgggtg cgcgcgtcga gttcgcgatc aagttgcccg gtcgacgtga
     3541 cgacaacgcg cccttgtggc tgccgatcga ctgcaagttt ccgcgcgacg actacgaacg
     3601 actcgtcgaa gcccaggagg ctgccgaccg ggtcggcgtc gagacctatt cgcgggcgat
     3661 cgagatgcgg ttgcgcaagg aggcgctgac gatccgcgag aagtatgtcg ccccgccgca
     3721 cacgaccgac ttcggcatcc tgttcctgcc gaccgagggc ctctatgccg aggcgctgcg
     3781 ccggccgggg ctcgtcgaga gcctgcagcg cgactacaag gtgatgctca ccggacc
//
LOCUS       DASRWS010000295         8608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_425585, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000295 DASRWS010000000
VERSION     DASRWS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425585"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFF72_05505"
     CDS             <1..761
                     /locus_tag="VFF72_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV92652.1"
                     /translation="RICLSVVSHGQGALVDRLLDDLSRLPAGTISRLVVTVNLPNDTW
                     ADPSGTAFHSVVVLRNSVPMGFSANHNQAFLHCEEPVFAVVNPDIRLDGNPFPALLDR
                     LSDHADFAVVAPLQVDAAGRLESFARTVPTPFNVAWRRLCQANGTPQTVRGRIEWLAG
                     AFMLWRAEAFRRLGGFDDRYRLYCEDVDICLRLQLQGQRFAVVETARVIHAAQRTSRA
                     SSRFLLMHVRSLLRLWLSPVFWRFVLTRPRALGA"
     gene            842..2077
                     /locus_tag="VFF72_05510"
     CDS             842..2077
                     /locus_tag="VFF72_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZV92653.1"
                     /translation="MTLLLWQGMGLAAGAALLWIARLEVPHTEPWRIFIHTLTWAWLA
                     SLGSAALLNVLIARQPYLHFTVTLVSVVAAFGVLFIWYIGTHATYSRWAIVIGLGFAI
                     TLHAYAWRRWRQRTTLKLGVLDEAMRERLAPASRFLSDGSDFERCDWRVLSDTMPFDQ
                     VDGVVLPDDQSAAIRQGAALRAKLAGAQVYSESFVRALLTGRLDIDRADEVFLDDVPT
                     SFVYASVKRCLDILGALALGLFALPVTLVCLALIRLESPGEALLVQPRVGLRGAVFGM
                     RKLRTMIEATEGLAEGDRHARITPLGRWLRRSRFDELPQLWNVLRGEMSLIGPRPEWT
                     ETAKALEAQIPQFAFRYLVRPGITGWAQVQQGHVTAEKAVRRKLEYDLYYAKNMSLAL
                     DLAIGAMTVRTVLTGSGAK"
     gene            2074..3006
                     /locus_tag="VFF72_05515"
     CDS             2074..3006
                     /locus_tag="VFF72_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZV92654.1"
                     /translation="MTGEPTRDCRIVAVLVAYRPEPELFAKVLAAIDDQVAAAIVVDN
                     GNGAGLEQIAFDERLERIDMHGNAGVAAAQNAGIRRALSAGATHVLILDHDSVSAFGM
                     TDKLLRATHDLEASGERVAAVGADYHDPRHPDSSPFVVMRPWGFGRVVSPTQADAVAV
                     PFLISAGSLIGADALTDVGLMEERLFIDYIDIEWCLRARGKGWTCHGVPGARLEHRLG
                     DAVLACGSSGRPVRIFGRTFSSRSPLRHYYMFRNGCWLVLHSALPLRWKLLETKRLVA
                     CFGVIAMFATQRRQQVRAMAKGVVHGIRGCLGPA"
     gene            3003..3503
                     /locus_tag="VFF72_05520"
     CDS             3003..3503
                     /locus_tag="VFF72_05520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV92655.1"
                     /translation="MMTRPIAVITVCRIPGVQLAAAIESVQALGDVRTKQIIIDGAST
                     DGTPAHLKSIESSLHHWSTAPDSGIYDAMNKGWAAAPADSYVIFIGAGDLLLGLPSAD
                     ELLQVERDGVAIIYGVTRTALGAFRSRYGAELRLRNTLHHQSLLVRKEAYLDPPFDTR
                     YRIYGD"
     gene            3816..4589
                     /gene="rfbF"
                     /locus_tag="VFF72_05525"
     CDS             3816..4589
                     /gene="rfbF"
                     /locus_tag="VFF72_05525"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HZV92656.1"
                     /translation="MKAVILAGGFGTRISEETQVRPKPMVEVGGRPILWHILKMYSAH
                     GIDDFIVCCGYKGYLVKEYFANYFLHMSDVTFDMTTNQMQVHQRKAEPWKVTLVDTGE
                     NTFTGGRLKRVASYLEGEEAFCFTYGDGVADLDISASVEFHKAHGKLATVTAVQPAGR
                     YGALALEGVSVRGFIEKPRGDGGWINGGFFVLSPKCLAGIENDESSWEGHDLPRIAAA
                     GELRAFEHRGFWQPMDTLRDKIQLEQLWASGAAPWKTWE"
     gene            4580..5647
                     /gene="rfbG"
                     /locus_tag="VFF72_05530"
     CDS             4580..5647
                     /gene="rfbG"
                     /locus_tag="VFF72_05530"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HZV92657.1"
                     /translation="MGMSRGFWVGRRVFLTGHTGFKGGWLALALHHAGARVSGYATDP
                     PTEPSLFAVARVASLLSNHTIADIRNAEALGVALRAASPEIVFHLAAQSLVRESYRNP
                     VETYAVNVFGSVNLLETVRGCSSVRAVVNVTSDKCYENVGTGRAHGEADALGGHDPYS
                     SSKACAELVTSAYRDSFLAAQGVTVATARAGNVIGGGDWASDRLVPDFLRALDAGRAA
                     SIRHPDAIRPWQHVLEPVSAYLLLAQALVEQGVARAGAWNFGPDEAEACTVSWMLDRL
                     TAQRPHARWERDVGCHPHEAPRLMLNSAKARKELGWQPHWDVAEAVDRTLEWHDAWRR
                     GDDMRALSLEQIEAHRQPVSA"
     gene            5653..6210
                     /locus_tag="VFF72_05535"
     CDS             5653..6210
                     /locus_tag="VFF72_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZV92658.1"
                     /translation="MGRLRICPTPLDGLTVVERSVDEDERGHFSRLFCAEELRGAGGE
                     PFAVAQINHSVTERAGSVRGLHFQWPPHGETKLVSCLRGAVFDVAVDLRRGSPTFLRW
                     HGEVLSRENRRAALIPEGFAHGFQTLEPGCELIYVHSRAYDPSAEGGLDPADTALAIA
                     WPLPFADVSARDRGHPRIGPAFVGL"
     gene            6221..7447
                     /locus_tag="VFF72_05540"
     CDS             6221..7447
                     /locus_tag="VFF72_05540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV92659.1"
                     /translation="MNCRHCGAELTLPLVDLGTAPPSNAYLSRAQLAAPERYFPLRVF
                     ACTGCWLVQTEDFADRSQLFAGDYAYFSSYSNSWLAHAEAFVDAAVARFALGTASLVV
                     EVASNDGYLLQYVKRKHIPCFGIEPTAGTARAAREKGIETQEAFFGTALAQSLSRDGH
                     TADLLIANNVLAHVPDINDFVTGFSHLLRPQGVASFEFPHLLRLIADCQFDTIYHEHY
                     SYLSLTAVGTIFERCGLAIFDVDELPTHGGSLRVYAQRADTGKRDPGPAVAELIARER
                     AAGLASPALAVGFQRRADRIKDGLLAFLIGQKRAGHTVAGYGAAAKGNSLLNYAGVRS
                     DLLAFVADASPHKQGSYLPGSRIPVVNEAVLRDERPAFVLILPWNLRSEIVQQLGYIA
                     NWGGRFATAVPEMQIS"
     gene            7444..8583
                     /locus_tag="VFF72_05545"
     CDS             7444..8583
                     /locus_tag="VFF72_05545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZV92660.1"
                     /translation="MSRIYYTKPSITELEVAYATDAVRNGWGEHCYDYIERFEAGFKK
                     HLGVSHAIGTSSCTGALHMGLAALGIGPGDEVIIGDINWIASAAPITYLGAKPVFVDV
                     LEDSWCLDPKRVAAAITPRTKAVIAVHLYGNLCDMDALLDIGARHGLPIIEDAAEAIG
                     SRIGDRAAGSMGSFGVFSFHGTKTITTGEGGMWVTREEALYERVRMLSNHGRSATQTR
                     QFWPDVLGFKYKMSNIEAAIGCAQLERINALVAGKRRVFRAYQERLRDLPLTMNVEPP
                     GTTNGYWMPSIVVTPGVPFDRQELLRDFRAAQIDARDFFWPLSQLPMFSPCPVNVVAY
                     GLSQRAVNLPTYHDLAEADIDRVAALVRKAVRGPERTHRGGPRHE"
BASE COUNT         1436 a         2838 c         2750 g         1584 t
ORIGIN      
        1 gacgaatctg cctttcggtg gtgagccacg gtcagggcgc gctggtagac cggctgctcg
       61 acgacttgtc gcgactcccc gcaggcacca tctctcggct tgtcgtcacg gtcaatctcc
      121 ccaacgacac ctgggccgat ccttcaggca cggctttcca ctctgtcgtc gtgctgcgca
      181 attcggtacc gatgggtttc agcgcgaacc ataaccaggc gttcttgcat tgcgaagaac
      241 ccgtgttcgc cgtcgtcaat cccgatattc gcctcgatgg caaccccttc ccggctctcc
      301 tcgatcgcct aagcgaccac gccgacttcg ccgtcgtcgc accgcttcaa gtcgacgcgg
      361 cggggagact cgaatcgttc gcgcggacag tgcctacccc tttcaatgtt gcatggcgac
      421 gactttgcca agccaacggc acgccgcaga cggtgcgcgg ccggatcgaa tggctcgccg
      481 gcgccttcat gctgtggcgc gccgaggcat tccgtcgcct tggcggcttc gacgatcgtt
      541 accgcctgta ttgcgaagac gtcgacattt gcctgcgtct gcaattgcag ggccagcgtt
      601 tcgcggtcgt cgagacggct cgcgtcatac atgcggcgca acgaaccagc cgggcgtcgt
      661 cgcgatttct gctcatgcac gttcgcagcc tgctacgcct ctggctgtcg ccggtgttct
      721 ggcgcttcgt gctcacgcgt ccgcgagctc ttggcgcatg agtcgccccg tgccgtcggc
      781 agccgtcggt gcctcgctgc gggccacgtc gcgtggcggc tccgtctggc aaagccgacg
      841 gatgacgctc ttgctgtggc agggaatggg actcgctgct ggcgccgcct tgctctggat
      901 cgcacgactc gaggtgccac acaccgagcc gtggcggatc ttcatccaca cgctgacctg
      961 ggcgtggctc gcatcactcg gctcggcggc attgctcaac gtcctgatcg cgcggcagcc
     1021 gtatctccac ttcacggtca cgctagtcag cgtcgtggcc gccttcggcg tactgttcat
     1081 ctggtatatc ggcactcacg ccacctactc gcgctgggcg atcgttatcg gcttggggtt
     1141 cgcgattacc ctgcatgcct acgcgtggcg gcgctggcgg cagcgcacga cgctcaagtt
     1201 gggcgtgctc gacgaggcga tgcgcgaacg gcttgccccg gccagtcgct tcctttccga
     1261 cgggtcggat ttcgaacgct gcgactggcg cgtcctctcc gatacgatgc cgttcgacca
     1321 ggtcgatggc gtcgtgcttc ccgacgatca aagcgcggcg atacgccaag gcgctgcgtt
     1381 gcgcgccaag ctggccggtg cacaggtgta ttcggagtca ttcgtacggg ctctgctcac
     1441 cggccgactc gacatcgatc gcgccgacga agtcttcctc gacgacgtgc cgacaagttt
     1501 cgtctatgcc tccgtcaagc gttgcctgga cattctcggt gcgctcgcgc tcggcttgtt
     1561 tgccctgcct gtcaccctcg tctgcctcgc cctgattaga ctcgagagtc cgggcgaggc
     1621 actgcttgtg cagccccgtg tcggcctgcg cggtgccgta ttcggcatgc gaaagctgcg
     1681 cacgatgatc gaggcgaccg agggcttggc cgaaggtgat cggcatgccc gcatcacgcc
     1741 gctcggccgc tggctgcgcc gctcccgctt cgacgagctt ccgcaactgt ggaacgtctt
     1801 gcgcggagag atgagcctga tcgggcctcg gcccgagtgg accgagactg ccaaggcgct
     1861 ggaggcacaa atcccgcagt tcgcctttcg gtatcttgta cgccctggca tcaccggctg
     1921 ggcgcaggtg cagcagggcc acgtcacggc cgaaaaggcc gttcgcagaa agctcgagta
     1981 cgacttgtat tacgccaaga acatgtcgct ggcgctggac ttagcgatcg gggcgatgac
     2041 ggtacggaca gtgctgacgg ggagcggcgc gaaatgaccg gcgaaccgac gcgggactgt
     2101 cgcattgtcg ctgtcctcgt cgcctatcgc cccgagccgg aattgtttgc gaaggtgctc
     2161 gccgcgatcg acgatcaggt cgcggcagcg atcgtcgtcg acaatggaaa cggggctggt
     2221 ctcgagcaaa tcgcattcga cgagcgactc gagcggatcg acatgcacgg caacgcgggc
     2281 gtcgccgccg cacagaacgc cggcatccgc agggcgttga gcgcgggtgc cacccacgtt
     2341 ctgatccttg accacgacag tgtctcagcg ttcggtatga ccgacaagct tcttcgagcc
     2401 acgcatgacc tcgaggccag cggtgaacgc gttgcggcgg tcggcgccga ttaccacgac
     2461 ccgaggcatc ctgactcgtc gccgtttgtc gtgatgcgcc cctggggatt cggtcgtgtc
     2521 gtctcaccca cccaggccga cgccgtcgcc gttccgtttc tcatcagcgc cggcagcctg
     2581 atcggcgccg acgctctcac cgacgttggc ctcatggaag agcgcctctt catcgactac
     2641 atcgatatcg aatggtgtct tcgcgctcgc ggcaagggat ggacctgcca cggcgtgccc
     2701 ggagcgcgtc tagagcatcg ccttggcgac gcggtactgg cctgcggcag tagcgggcgg
     2761 ccggtgcgca tcttcggccg cacgttttcc tcgcgcagtc ctctccggca ctactacatg
     2821 tttcgcaacg gttgctggct ggttctccac agtgcgctgc cgctacgatg gaagcttctc
     2881 gagaccaagc gcctcgtcgc ctgcttcggt gtcatcgcga tgttcgccac acaacgccga
     2941 caacaggttc gggcgatggc caaaggcgtc gtgcatggca ttcgcggatg cctcgggccg
     3001 gcatgatgac tcggcccatt gcggtcatca cggtctgcag aatcccgggc gtccagctcg
     3061 ctgccgcgat cgagagtgtg caagcactcg gcgatgtcag gaccaagcag atcatcatcg
     3121 atggggcgtc cacggacggc acgccggccc atctgaaatc catcgaaagt tccctgcacc
     3181 actggagcac tgcgccggac tcgggcattt acgatgcgat gaacaaagga tgggccgccg
     3241 cccccgccga ctcgtacgtg atcttcatcg gcgcgggcga tctgctactc gggctgccgt
     3301 cggcggacga gctactgcaa gtcgagcggg atggcgtcgc gatcatttac ggcgtgacgc
     3361 gaaccgcgct cggtgcgttc cgatcgcggt acggagccga gctgagattg cgcaacaccc
     3421 tccaccacca gtcactgctg gttcgcaagg aggcgtacct ggacccgccc ttcgacacgc
     3481 gctatcggat ctacggtgac tgacctcaac attcgaatgt ggcgtgccgg attgcaggcg
     3541 gcgcattgcg agtccctcca ggcgtcagcc ggcgccggcg gggccagcgc gatgcagccc
     3601 ttgggcgaga cctttttatc gcccgacgaa actcgggcgt gctgctcgcc ggtgcggcat
     3661 ggtcgctcgt cgccggaagc cgcctgcgtc gcagtgcgac gaaaaagctg cgccatttca
     3721 cggatcgatt tggcattcgc gcctgaatca caccctgcgt tgatcgaacg ccaaatccgg
     3781 ccatatagag ttgcacacca tcgctgagga cgcgcatgaa ggcagtcatt cttgccggtg
     3841 gatttggaac ccgcatttcc gaagaaacac aggttcgacc gaagccgatg gtcgaggttg
     3901 gcggccgccc gatcctgtgg catatcctga aaatgtattc agctcacggc atcgacgact
     3961 tcatcgtctg ctgtggctac aagggctact tggtgaagga gtacttcgcc aactatttcc
     4021 tgcacatgtc cgatgtcacg ttcgacatga caaccaacca aatgcaggtt caccagcgca
     4081 aggccgagcc atggaaggtg acgctcgtcg acaccggcga aaacactttc accggaggcc
     4141 ggctaaagcg agtcgcctct tatctcgaag gggaagaggc gttctgcttc acctatggtg
     4201 acggcgttgc cgacctcgac atctcggcga gcgtcgagtt tcacaaggca catggcaagc
     4261 tcgcgacggt caccgcggta cagcccgccg gccgctacgg ggcgctggcg ctagaaggcg
     4321 tttcggtccg tggcttcatc gaaaagccgc gcggcgacgg cgggtggatc aatggtggct
     4381 tcttcgttct ctcgccgaag tgtctggcgg gcatagagaa cgacgaatcg agctgggagg
     4441 gacacgacct gccgcgcatc gccgccgccg gcgagttgcg ggcgttcgag caccgtggct
     4501 tctggcaacc catggacacc ctccgcgaca agatccagct cgaacagctg tgggcctcgg
     4561 gcgctgcgcc ctggaagaca tgggaatgag ccgcgggttc tgggtgggac ggcgtgtctt
     4621 cctgaccggc cacacgggct ttaagggggg ctggctggcg ctggcgcttc accacgccgg
     4681 cgcgcgggtc tccggatacg cgacggaccc gcctaccgag ccgagcctgt tcgccgtcgc
     4741 ccgcgtcgcg agcctgctgt cgaaccacac gatcgccgac atacgcaatg ctgaggcttt
     4801 gggtgtcgcg ctgcgcgcgg catcgcccga aatcgtgttt catctcgccg cgcagtcact
     4861 ggtgcgcgag tcgtaccgca atccagtgga gacgtatgcg gtcaacgtct tcggcagcgt
     4921 caatcttctc gaaacggtgc gcggctgctc gtcggtgcgc gccgtcgtca acgtcacttc
     4981 ggacaagtgc tacgagaacg tcggcaccgg ccgcgctcat ggcgaggccg atgcgctggg
     5041 cggccacgat ccctactcga gcagcaaggc ctgcgccgag ctggtcacct ccgcctaccg
     5101 cgattcgttc ctcgcggcac agggcgtgac cgtggcgacg gcgcgcgccg gcaacgtgat
     5161 cggcggcggc gactgggctt cggatcggct cgttcccgat ttcctgcgcg ctctggatgc
     5221 gggacgcgcc gcgtccatcc ggcatcccga cgccattcgt ccctggcagc atgtgctcga
     5281 gccggtttcg gcgtacctcc tgttggcaca ggccctcgtc gaacaaggcg tggcgcgtgc
     5341 cggagcctgg aacttcggcc cggacgaagc cgaggcgtgt acggtcagct ggatgctcga
     5401 tcgcctgacg gcacaacggc cgcatgcgcg ctgggagcgc gacgtcggtt gtcatcctca
     5461 cgaagcgccg cgactcatgc tgaacagcgc gaaggcacgc aaggagctcg gctggcagcc
     5521 gcactgggac gtggctgaag cggtcgaccg aacgctcgaa tggcacgacg cctggcgacg
     5581 cggcgacgac atgcgcgcgc tgagcctcga gcagatcgag gcgcaccggc aaccggtcag
     5641 cgcatgacga cgatgggtcg acttcgcatt tgcccgacgc cgctcgacgg cctcaccgtt
     5701 gtcgaacgca gcgtcgatga agacgaacgc ggtcatttca gccggctgtt ctgcgccgaa
     5761 gagctgcgcg gtgcgggcgg cgagcccttt gccgtcgccc agatcaacca cagcgtcacg
     5821 gaacgggccg gctcggtgcg ggggctgcat ttccagtggc ctcctcacgg cgagaccaag
     5881 ctggtgagct gtctgcgcgg cgccgtgttc gacgtggccg tggacttgcg gcgcggatcg
     5941 cccactttcc tgcgctggca tggcgaagtc ttgtcgcgcg agaaccggcg tgcggcgctg
     6001 atcccggaag gattcgcgca cggcttccag acactggagc cgggctgcga attgatctac
     6061 gtgcattcac gcgcctatga cccgagcgcc gagggcggcc tcgacccggc cgacacggcg
     6121 ctggcgatcg cctggccgct gccgttcgcc gatgtgtcgg cgcgcgatcg cggccatccg
     6181 cgcatcggcc cggcatttgt cggcctgtga gacgcagacg atgaactgcc gccactgtgg
     6241 ggccgagctc acgctgcctc tggtcgatct gggcaccgca ccgccctcga acgcctatct
     6301 gagtcgcgct caactggccg cgcccgaacg ctacttcccg ctgcgcgtct tcgcctgcac
     6361 cgggtgctgg ctggtgcaga ccgaggattt cgccgaccgc tcgcaattgt tcgccggcga
     6421 ctacgcctat ttcagctcgt actcgaacag ctggctcgcc cacgcggaag ccttcgtgga
     6481 cgcggccgtg gcccgcttcg cgctcggcac cgcgtcgctg gtcgtcgagg tagcctcgaa
     6541 cgacggctac ctgcttcagt acgtgaagcg caagcacatt ccttgcttcg gcatcgagcc
     6601 gaccgccggc acggcgcggg cggctcgcga gaagggaatc gagacccagg aagccttttt
     6661 cggcacagcc ctcgcgcaat cgctgtcgcg cgatgggcac accgccgacc tgctgatcgc
     6721 gaacaacgtg ctcgctcacg tgccggacat caacgacttc gtcaccggct tttcgcacct
     6781 cctcaggccg caaggcgtcg ccagcttcga gttcccgcac cttctgcggc tcatcgccga
     6841 ctgccaattc gacaccatct atcacgagca ctactcgtac ttgtcactga cggctgtcgg
     6901 cacgatcttc gaacgctgtg gtcttgcgat cttcgacgtc gatgagttgc cgacgcacgg
     6961 cggcagcctt cgcgtctacg cccaacgggc cgatacaggc aagcgcgacc ccggcccggc
     7021 tgtagccgag ctgatagcgc gcgagcgggc ggccggtctg gcgtcgcccg cgctggccgt
     7081 aggatttcag cggcgtgccg atcgcatcaa ggacgggctc ctcgcgttcc tcatcgggca
     7141 gaagcgcgcc ggtcacacgg tcgcggggta tggcgcggca gccaagggca acagcttgct
     7201 caactatgcg ggcgtgcgtt cggacctgct ggcattcgtc gccgacgcgt cgccgcacaa
     7261 acaagggtcg tacttgccag gctcgcgaat tccggtcgtg aacgaagcgg tcttgcgcga
     7321 cgagaggccc gcgttcgtcc tgatactgcc gtggaatctg cgcagcgaaa tcgtgcagca
     7381 actcggctac atcgcaaact ggggcggccg cttcgcgacc gccgtgcccg agatgcagat
     7441 ctcatgagcc gcatctacta caccaagcct tcgatcacgg aactcgaggt ggcctacgcg
     7501 accgacgcag tgcgcaatgg ctggggcgag cactgttacg actacatcga gcgcttcgag
     7561 gccggcttca agaaacacct cggcgtgagc cacgccatcg gcacctcgag ttgcaccggc
     7621 gcgctgcaca tgggtctagc cgcgctggga atcggacccg gcgacgaagt gatcatcggc
     7681 gacatcaact ggatcgcctc ggccgcgccg atcacctatc tcggcgcgaa gccggtgttc
     7741 gtcgacgtgc tggaagacag ctggtgcctc gacccgaagc gcgtggcggc cgccatcacg
     7801 cccaggacca aggccgtcat cgccgttcat ctgtatggaa acttgtgcga catggatgcg
     7861 ttgctcgaca tcggggcacg gcacggattg cccatcatcg aggacgccgc cgaagcgatc
     7921 ggctcgcgca tcggcgatcg cgccgcaggt tcgatgggct cctttggcgt cttctctttt
     7981 cacggcacga agacgatcac caccggcgaa ggcggaatgt gggtgacgcg ggaagaggcg
     8041 ttgtacgaac gcgtgcgaat gctgtccaac cacggccgct ccgcgacgca aacgcgacag
     8101 ttctggccgg acgtgctcgg cttcaagtac aagatgtcaa acatcgaagc ggcgatcggg
     8161 tgcgcccagc tcgagcgcat caacgcgctt gtcgcgggta agcgcagggt gtttcgtgcc
     8221 taccaggaac gcctgcgcga tctgccgctg accatgaacg tggaaccccc cggaacgacc
     8281 aacggctact ggatgccgag catcgtggtc accccgggcg tgccgttcga tcgccaagag
     8341 ctgcttcgcg actttcgggc cgcgcagatc gatgcacgcg atttcttctg gccattgagc
     8401 cagctgccga tgttctcgcc ctgcccggtg aatgttgtgg cctacggcct ttcgcagcgt
     8461 gcagtgaatc ttccgaccta tcacgacctc gctgaggccg acatcgaccg ggtggctgcc
     8521 ttggttcgca aagcggtgag gggaccagag cgaacgcacc gcggagggcc tcgacatgag
     8581 tgacggggaa ttcctgagca acgagcag
//
LOCUS       DASRWS010000296         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1566889, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000296 DASRWS010000000
VERSION     DASRWS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566889"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..888
                     /gene="ugpQ"
                     /locus_tag="VFF72_05550"
     CDS             118..888
                     /gene="ugpQ"
                     /locus_tag="VFF72_05550"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZV92661.1"
                     /translation="MTAFKPSDWPCPRWIAHRGAGKLAPENTLAAFRIGAVHGFRAFE
                     CDVKLSADEVPFLLHDTNLQRTTDARGRAADRPWHELSRIDAGGWHSRTFAGEPLPSL
                     EAIARFCLRNGFFLDLEIKPTPGSEELTGRIVAEHVERLWGSAPTLPLLTSFAPVALD
                     AARRTRPDLPRGLLLDSLRPGWLDEAQGLGCKTVVTNYSVADAAMIASIHDRDMKALV
                     YTVNDPAAAKRLIALGIDGLVTDAVDRFSPGVEDAIAD"
     gene            complement(930..1535)
                     /locus_tag="VFF72_05555"
     CDS             complement(930..1535)
                     /locus_tag="VFF72_05555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZV92662.1"
                     /translation="MQASIEPLLIDVPTRIETARLILRSPRQGDGAAVNAAVIASHHE
                     LDKWLPWAIDLPTLDESEAHCRRQQARFILREDLAMFIFAREPDGGEGELLGGTGLHR
                     IDWALRRFEIGYWRRSGLEGRGIVTEAVRALSRMAFDVLAARRVEVRMDNDNERSWKV
                     AERAGFTLEAVLRFDSSTPAGEPRSTRVYARVRGAEEPVGP"
     gene            complement(1543..>2528)
                     /locus_tag="VFF72_05560"
     CDS             complement(1543..>2528)
                     /locus_tag="VFF72_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92663.1"
                     /translation="YVCSFVIGIVYTVAGSAAQIVLMQVVARDRLVEAHAKNALANSG
                     AEVCGPGVAGVLLKLVGAPLALLVDAVLVSISALILRGLAIQEQPAPRRGTEGAAVQF
                     WGELRAGVRFVGHHRLLVTLAVVVGAWQACYNAALVVQILFATRTLGLSEQAVGLSYT
                     AMGVGTVFASVIGDRVSRRLGPGPCLLAGIAICGCGWLLLSLAPASAWGVAAFAATLM
                     MFSTGAVFIFINFLALRQAVTPAPLLGRMTSTMRWLILVPAGPGALLGGWLGQHSGLR
                     TSLAVAGVGSLLLAFAASRVGVVRGIKRLPSPDEVEAWLDAEAGVAPAAIV"
BASE COUNT          427 a          912 c          848 g          341 t
ORIGIN      
        1 agcttccgca gcctcgacca ccttcgcaga aatcgtcatg gagttgaagt ccctcaccgc
       61 cgagttcatc ggtcggtcgc cgatgaacct cgttcgcggc gccgcgcgca tcggcacatg
      121 accgccttca aaccgagcga ctggccctgc ccgcgctgga tcgcgcaccg cggtgccggc
      181 aagctggcgc cagagaacac gctggcggcg tttcgcatcg gcgccgtgca cggctttcgc
      241 gcgttcgaat gcgacgtcaa gctgtccgcc gacgaggtgc cgttcctcct gcacgacacg
      301 aacctgcagc gcaccaccga tgcgcgcggt cgcgccgccg acaggccttg gcacgagctg
      361 tcgcgcatcg acgccggcgg ctggcacagc cggacgttcg ccggcgagcc gttgccctcg
      421 ctcgaggcga tcgcccgctt ctgccttcgc aacggctttt tcctcgacct cgagatcaag
      481 ccgacgccag gcagcgaaga actcaccggg cggatcgtcg ccgagcacgt cgagcggctg
      541 tgggggagcg caccgacgct gccgctgctc acctcgttcg ctccggtggc cctcgatgcg
      601 gcgcgacgta cccggccgga tctgccgcgc ggactcctgc tcgattcttt gcgaccgggc
      661 tggctcgacg aggcgcaagg gctcggctgc aagaccgtgg tgacgaacta ttcggttgcc
      721 gacgccgcca tgatcgccag catccacgac cgcgacatga aggcgctggt ctacaccgtc
      781 aacgatccgg ccgccgcgaa gcgtttgatc gcgctgggca tcgacggcct cgtcaccgac
      841 gccgtcgacc gattctcgcc gggagtcgaa gacgccatcg ccgactgagc cgcgacgtcc
      901 cgtcgccccg cctctcgtcg ggcgatcgtt caggggccga ccggctcctc ggcgccgcga
      961 acgcgggcat agacccgggt gctgcgaggt tcgcccgccg gtgtcgacga atcgaatcgc
     1021 agcacggcct cgagcgtgaa gccggcgcgc tcggcgactt tccagctgcg ctcgttgtcg
     1081 ttgtccatcc ggacctcgac acggcgggcg gcaagcacgt cgaaggccat ccggctgagc
     1141 gcccgcacgg cctccgtcac gatgccgcgc ccctcgaggc cggaccttcg ccagtagccg
     1201 atctcgaaac gacgcagcgc ccagtcgatc cggtgcagcc cggtgccgcc gagcagctcg
     1261 ccttcgccgc catcgggctc gcgcgcgaag atgaacatcg cgaggtcttc gcgcaggatg
     1321 aagcgggctt gctggcggcg gcagtgggcc tccgactcat cgagcgtcgg caggtcgatc
     1381 gcccagggca gccacttgtc gagctcgtgg tgcgacgcga tcactgccgc attgacggcc
     1441 gctccgtccc cttgccgcgg gctgcgaagg atcagccgcg ccgtctcgat gcgcgtcggc
     1501 acatcgatca gcagcggttc aatcgacgcc tgcacgatcc gctcacacga tggcggccgg
     1561 ggcgacgccc gcttcggcgt ccagccaagc ctcgacctcg tcgggcgacg gcagtctctt
     1621 gatgccgcga acgacgccca cgcgcgatgc cgcgaaggcg agcagcaacg agccgacacc
     1681 cgcgaccgca agcgaggtgc gcagtcccga gtgctggcca agccagccgc caagcaaagc
     1741 gccggggccc gccggaacga ggatcagcca acgcatcgtg cttgtcatgc gcccgagcag
     1801 gggcgcaggc gtcaccgcct ggcgcagggc caggaaattg atgaagatga agacggcgcc
     1861 ggtcgagaac atcatcaggg tcgcggcgaa cgcggcgacg ccccaggcgc tggccggcgc
     1921 cagcgacaac agcaaccaac cgcagccgca gatggcgatg cccgcaagca ggcagggccc
     1981 ggggccgagg cgtcggctga cacggtcgcc gatgacgctg gcgaacaccg tgccgacccc
     2041 catcgcggta tagctcagcc cgaccgcctg ctccgaaagg ccgagggtgc gcgtcgcaaa
     2101 gaggatctgc acgacgagag cggcgttgta gcaggcctgc caggcaccga cgacgaccgc
     2161 gagcgtgacg agcaggcggt gatgcccgac gaaacggacg ccggcgcgca actcgcccca
     2221 gaactgcacc gccgcgccct cggtgccccg tcgcggtgcc ggttgctcct ggatggcgag
     2281 gccgcgcagg atcagcgccg agatcgagac gagcacggcg tcgacgagca acgccagcgg
     2341 tgcgccgacg agcttgagga gaacaccggc gacgcccgga ccgcacactt ccgcccccga
     2401 gttggcgagc gcgttcttcg catgcgcctc gaccagtcgg tcgcgcgcaa cgacctgcat
     2461 caggacgatc tgggcggcac tgccggcgac ggtgtagacg atgccgatga cgaacgagca
     2521 gacataga
//
LOCUS       DASRWS010000297         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2409370, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000297 DASRWS010000000
VERSION     DASRWS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409370"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFF72_05565"
     CDS             complement(<1..455)
                     /locus_tag="VFF72_05565"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZV92664.1"
                     /translation="MAQHFEVHPTNPQPRLLRQAVQILHDGGVAAIPTDSSYALVCHL
                     DDKAAAEQLRRLRGVDGRHHLTLLCRDLSEVASYARVDNKQYRLLKASTPGPFTFILE
                     ATREVPRRVSHPSRRTIGLRVPEHPVTSQLLELLGQPLLATTLIAPGES"
     gene            complement(483..1334)
                     /locus_tag="VFF72_05570"
     CDS             complement(483..1334)
                     /locus_tag="VFF72_05570"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HZV92665.1"
                     /translation="MNADLHCHSTVSDGTLDPEAVAARASANGVELWALTDHDEIDGQ
                     QRALAAARTLGLPYLTGTEISVTFAGTTVHIVGLGFDPADERLVRGLAATRGGREARA
                     REMADALAKVGIADAFEGALRFVGNPELISRTHFARFLVDSGACADTQEVFRRFLVEG
                     RPGFVPHRWATLRDAVTWISDAGGVAVVAHPARYKFTATEEYALFTEFKTLGGRGVEV
                     VTGAHSAADARRYADTAREFDLLASRGSDFHSPEESRIDIGTLPPLPEGLEPVWKALE
                     PRIHAPG"
     gene            complement(1404..>2077)
                     /locus_tag="VFF72_05575"
     CDS             complement(1404..>2077)
                     /locus_tag="VFF72_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZV92666.1"
                     /translation="VAPAFAVFHESFHEGVVGIIASRLKDRLHRPTFVFARGQDGDLK
                     GSGRSIEGLHLRDALDLVSKRLPLLLRRFGGHSMAAGCTIARADLDAFRAALSDATLA
                     LGTAPGVRRLATDGPLPAEHWHAETVRLIGQAVWGSAFEAPVFCDAVDVVSQRLVGEK
                     HLKLSLRLNGALRDAIWFGRVEPVADRLRLAYRLELDSYNGDERLQMIVVAAEPARGA
                     ASPSA"
BASE COUNT          294 a          696 c          772 g          315 t
ORIGIN      
        1 tccgactcgc cgggtgcgat cagcgtcgtc gccagcagcg gctggccgag cagctcgagc
       61 agctggctcg tcaccggatg ctcgggaacg cgcaggccga tcgttcgcct cgacgggtgc
      121 gagacgcgcc gcggaacctc ccgcgtcgcc tcgaggatga aggtgaacgg gcccggggtc
      181 gatgccttca gcagccggta ttgcttgttg tcgacgcgcg cgtagctggc gacctcggac
      241 aggtcgcggc agagcagcgt caggtgatgt cgtccgtcga cgccgcgcag tcgtcgcagc
      301 tgttcggcgg cggccttgtc gtcgaggtgg cagacgagcg cgtagctgga gtcggtgggt
      361 atcgcggcga cgccgccatc gtgcaggatc tgcacggcct gccggagcag cctcggctgc
      421 gggttcgtgg ggtggacttc gaagtgctgc gccatccctc gattgtcgac gccgcggtca
      481 gatcagccgg gggcgtggat tcgcggctct agggctttcc acaccggctc gagaccttcg
      541 ggcagcggcg gcagggtgcc gatgtcgatg cggctctcct cggggctgtg gaagtcgctg
      601 ccgcgcgagg cgagcaggtc gaactcgcgc gccgtatcgg cgtagcgcct cgcatcggcg
      661 gcgctgtgcg cgccggtcac cacctcgacg ccgcgcccgc ccagtgtttt gaactcggtg
      721 aagagcgcgt attcctcggt cgccgtgaac ttgtagcgcg ccggatgggc gacgacggcg
      781 acgccgccgg cgtcgctgat ccaggtcacc gcgtcgcgca gcgtcgccca gcggtgcggc
      841 acgaagccgg gcctgccttc gaccaggaag cggcggaaca cctcctgggt gtcggcgcag
      901 gcaccgctgt cgacgagaaa gcgcgcgaag tgggtgcgcg agatgagctc ggggttgccg
      961 acgaaccgca aggcgccctc gaacgcatcg gcgataccga ccttggcgag cgcgtcggcc
     1021 atctcgcggg cgcgcgcctc gcggcctcca cgcgtcgccg ccaggccgcg caccagccgc
     1081 tcgtcggccg ggtcgaagcc gaggccgacg atgtgcaccg tcgtcccggc gaaggtcacc
     1141 gagatctcgg tcccggtgag gtacggcaat ccgagcgtcc gggctgcggc gagcgcgcgc
     1201 tgctggccgt cgatttcgtc gtgatcggtc agcgcccaga gctcgacgcc gttggcgctg
     1261 gcgcgcgccg ccaccgcttc aggatcgagc gtgccgtcgg agacggtcga gtggcagtgg
     1321 aggtcggcgt tcatgagcac ctgcgattcg tcgcatcata gtggccgctt tccgatcgtg
     1381 tcggctggcg tggatgctgc ggatcaggcc gaaggcgatg cagcgccgcg tgccggctcg
     1441 gccgcgacga cgatcatctg cagtcgttcg tcgccgttgt agctgtcgag ctcgagccgg
     1501 taggccaggc gcaatcggtc ggcgacgggc tcgacccggc cgaaccagat ggcatcgcgc
     1561 agcgcgccgt tcaggcgcag cgagagcttc aggtgctttt cgccgacgag ccgctgcgag
     1621 acgacgtcga ccgcgtcgca gaagaccggc gcctcgaagg ccgagcccca gaccgcttgg
     1681 ccgatcaggc gcaccgtctc ggcgtgccag tgctcggccg gcagcggtcc gtcggtggcg
     1741 agtcgccgca cgcccggcgc ggtgccgagc gccagcgtgg cgtcgctcaa ggcggcgcga
     1801 aaggcgtcga ggtcggcgcg cgcgatggtg cagccggccg ccatcgaatg gccaccgaaa
     1861 cgcctgagca gcagcggcag tcgcttgctc acgaggtcga gcgcgtcgcg caggtgcaac
     1921 ccttcgatcg agcggcccga gcccttcagg tcgccgtcct ggccacgtgc gaagacgaat
     1981 gtcggccggt gcaggcgatc cttcagtcgg gacgcgatga tgccgaccac gccttcgtgg
     2041 aagctctcgt ggaagacggc gaacgccggc gccacgc
//
LOCUS       DASRWS010000298         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4155089, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000298 DASRWS010000000
VERSION     DASRWS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4155089"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFF72_05580"
     CDS             <1..399
                     /locus_tag="VFF72_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZV92667.1"
                     /translation="DWLEAEPGRCVAKAYDVVLNGVELGGGSVRIHKEDVQSKVFRAL
                     KIDAEEAQAKFGFLLDALQYGAPPHGGIAIGLDRFVMLMTGAESLRDVIAFPKTQRAQ
                     DLLTNAPGPVDEKQLRELHIRLRTAAPAAA"
     gene            413..883
                     /gene="nudB"
                     /locus_tag="VFF72_05585"
     CDS             413..883
                     /gene="nudB"
                     /locus_tag="VFF72_05585"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HZV92668.1"
                     /translation="MAQASRHKIPESVLVVIHSAALDVLLLERADMPGFWQSVTGSKD
                     RAGEPLAETCRREVGEETGIVIGGERVHADCLRDWHMRNVYDIYPHWQHRYAPGVTRN
                     TEHVWGLVVPRDVEVRLAPREHLRFEWLPWQRAAERCSSPSNAEAILHLPRLHG"
     gene            916..1710
                     /locus_tag="VFF72_05590"
     CDS             916..1710
                     /locus_tag="VFF72_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZV92669.1"
                     /translation="MRLPSTITTTQGANVLRVATYNIHKGVRGLGPRQRLEIHNLVLG
                     IESLDTDLVFLQEVRRMNRAAARHFPDTKIGWPKVPQAEYLAPEGYEVAYRTNAVTRH
                     GEHGNALLSRWPIGEVGHHDVSDHRFEQRGLLHVPVTWNGTPMHSIVAHFGLIHSSRV
                     RQAQRLREFIVANVPAGEPLLVAGDFNDWGERLDELMAHCELARACIPGDPPSRFNTF
                     PSRVPLFSMDRIYMRGFRCVSTSVPRGTAWARMSDHLPLIAELELT"
     gene            1719..>2128
                     /locus_tag="VFF72_05595"
     CDS             1719..>2128
                     /locus_tag="VFF72_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZV92670.1"
                     /translation="MARSPSRAEASRALLGGHRITLIKGGVAFFAALVEAIDAARAEV
                     LLETYIFDFHGGAVAIAEALERAAARKLTVRVVVDGFGTPAIPVDWQERWTRAGVHWR
                     VFSPGGSLSLLRPGHWRRLHRKLAVVDAAVAFCGG"
BASE COUNT          310 a          761 c          713 g          344 t
ORIGIN      
        1 gactggctcg aagccgagcc gggtcgttgc gtcgccaagg cctacgacgt ggtgctcaat
       61 ggcgtcgagc tcggcggcgg ctcggtccgg atccacaagg aagacgtgca gagcaaggtc
      121 ttccgggcct tgaagatcga cgccgaggag gcccaggcca agttcggctt cctgctcgac
      181 gcgctgcagt acggcgcgcc gccgcacgga ggcatcgcca tcggcctcga tcgcttcgtg
      241 atgctgatga cgggcgccga atcgctgcgc gacgtgatcg cctttccgaa gacccagcgg
      301 gcgcaggatc tgctcaccaa tgccccgggt ccggtcgacg agaagcagtt gcgcgagctg
      361 cacatccggc tgcgcacggc ggcgccggcg gccgcctgag acgggcgcgg cgatggcgca
      421 ggcctcccgg cacaagattc ccgaatcggt gctggtggtc atccacagcg cggcgctcga
      481 cgtgctgctg ctcgagcgcg ccgacatgcc cggattctgg caaagcgtca ccggctcgaa
      541 ggatcgtgcc ggcgagccgc tggccgaaac ctgccggcgc gaagtcggcg aagagacggg
      601 catcgtcatc ggcggcgagc gtgtgcacgc cgattgcctg cgggactggc acatgcgaaa
      661 cgtctacgac atctacccgc actggcagca ccgctatgct cccggggtga cccgcaatac
      721 cgagcatgtc tggggcctcg tcgtgccgcg cgacgtcgag gtccggctgg cgccacgcga
      781 acacctgcgc ttcgaatggc tgccctggca acgagccgcc gagcgatgca gctcgccgtc
      841 gaacgccgag gcgatcctgc acctgcctcg gttgcacgga tgactctcct tcccgttccg
      901 gctccgttgc cttccatgcg ccttccgtcc acgatcacca cgacccaggg cgcgaacgtg
      961 cttcgcgtcg ccacctacaa catccacaag ggcgtgcgcg gcctgggccc gcgccagcgg
     1021 ctcgagatcc acaacctcgt gctcggcatc gagtcgctcg acaccgacct ggtcttcctg
     1081 caggaggtgc ggcggatgaa ccgcgccgcg gcgcgtcatt tccccgatac caagatcggc
     1141 tggccgaagg tgccgcaggc ggagtacctg gcgcccgagg gctacgaggt cgcctaccgc
     1201 accaacgcgg tgacgcgcca cggcgagcac ggaaacgcgc tgctgtcgcg ctggccgatc
     1261 ggcgaagtcg gccatcatga cgtttccgat caccgcttcg agcaacgcgg cctgctccac
     1321 gttcccgtca cctggaacgg cacgccgatg cattcgatcg tcgcccattt cggcctcatc
     1381 cattcgagcc gggtccgcca ggcgcagcgg ctgcgcgaat tcatcgtcgc caacgttccg
     1441 gccggcgagc cgctgctcgt cgccggtgac ttcaacgact ggggcgagcg gctcgacgag
     1501 ctgatggccc actgcgagct ggcgcgcgcc tgcatcccgg gcgacccgcc gtcgcgcttc
     1561 aacacctttc cgtcgcgggt gccgctcttc tcgatggatc gcatctacat gcgcggcttt
     1621 cgctgcgtgt cgacgtcggt gccgcgcggc acggcatggg cacgcatgtc agaccatctg
     1681 ccgctcatcg ccgagctcga gctcacctga tcgcgcgcat ggccaggtcg ccgtcgcgtg
     1741 ccgaggcctc gcgagccctc ctcggcggcc atcggatcac cttgatcaag ggcggcgtcg
     1801 cgttcttcgc ggcgctggtc gaggcgatcg acgcggcgcg cgccgaggtg ttgctcgaga
     1861 cctacatctt cgatttccac ggcggcgccg tcgcgatcgc cgaggcgctg gagcgcgccg
     1921 cggcgcgcaa gctcacggtg cgtgtcgtcg tcgacggttt cggcacgccg gccattccgg
     1981 tcgactggca ggagcgctgg acgcgggcgg gcgtccactg gcgggtcttc agccccggcg
     2041 gcagcctgag cctgcttcgt cccggccact ggcgccgcct ccaccgcaag ctcgccgtcg
     2101 tcgacgctgc ggtcgccttt tgcggtgg
//
LOCUS       DASRWS010000299         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_702736, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000299 DASRWS010000000
VERSION     DASRWS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702736"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2552)
                     /locus_tag="VFF72_05600"
     CDS             complement(114..2552)
                     /locus_tag="VFF72_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HZV92671.1"
                     /translation="MSLGRVGPLASVSALARAGTKSAAWIVVVLVAIATFTAAAWAQT
                     AAVPESEPASAPAPSAPASAASGIQLRQAAALQPLPRGDAGRQLPIILRDREIHGRPD
                     LDAEAVGDVEFRRGDVVVRADRLTYDQTDDIARASGHVVISRDGNVFSGPELQLKVER
                     FEGFFESPSYRFAKTHAGGKATRIDFIDDQRSIAFDATYSSCTVEDPQEPAWILKAQS
                     IRIDNEINEGVAKNAVLRFYGVPILASPVLSFPLNEERKSGFLPPSIGLDSRSGVQSA
                     LPYYWNIAPNRDATFTASDSLRRGPGLETEFRYLEPEYSGEADFKLLPHDDTVGRSRY
                     SLRIAHEGTLPFGAYAQAHVLRVSDDDYWKDFPDELKTTTQRLLQTDLQVSRPFGDWS
                     TYARVERWQLLQTVDPTTRIDPAPYERMPQIGARYAAPWAHGFEVGFETEFNRFSNPD
                     DTYLLPRQTGLRVDALGSISRPFYSPGWTLTPKLSFNAASYALDMPLADGRRSASRVI
                     PTLSIDSAWTLERETTLFGRDVRQTLEPRLFYVDTPYVRQDGLPNFDSFAKDFNFDSV
                     FTENSFSGVDRVSDSHQLTAGVTTRFLDPQSGAESMRLGIAQRYLFRDQLVTPDGPPL
                     TQRFSDILVLGSTSIVPHWNFDADAQYNPDSHRIEQSLAGFRYSPGPFRTVSFNYRLS
                     RGLAEQVELGWQWPVYGRARGSDRNRPAGGGGCGGTLYSVGRVNYSTRDSRLTDSIVG
                     FEYDAGCWIGRIVVERLSTGLSEATTRLLLQLELVGLSRLGSNPLHVLKDNIPGYQLL
                     REERDTNTPFTPYD"
     gene            2781..3818
                     /locus_tag="VFF72_05605"
     CDS             2781..3818
                     /locus_tag="VFF72_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZV92672.1"
                     /translation="MAWADPAREAAFLDWLGCVASRHDLVVATLRQASSDASFRRYLR
                     VDRADGGSMVVMDAPPPEENVRPFLHVAGLIERAGLHAPHVFEADPEHGFVLLEDLGG
                     TLYLEALLATQQDDDPQRADSLMQVAIRALVAWQRDLPATTLPAYDDALLRRELAIFP
                     EWCVCREHRVTWTDAEAETWARACNLLVASALAQPVVTVHRDWMPRNLMVSDPNPGIL
                     DFQDAVRGPIAYDVASLLRDAFVSWDEEREIDWAIRYWEGARKAGLPVRDDFGEFWRE
                     LEWIGLQRHLKVLGIFCRLKHRDDKPAYSADLPRFFAYAHRVSTRYSALRPLSRLLEP
                     LMGVSRIEAFH"
     gene            3834..>4460
                     /locus_tag="VFF72_05610"
     CDS             3834..>4460
                     /locus_tag="VFF72_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92673.1"
                     /translation="MLRLTELRLPLEHTQAELRAAVLGRLGIDESSLGSWRVFRRGYD
                     ARKRGAIVLVYTIDCEIEDDAAVLGAHAGDPNLRPAPDTTYRFVAHSDSRQRGPRPVV
                     VGFGPCGLFAALILAQSGFRPLVIERGKAVRERTRDTWGLWRRGVLDPESNVQFGEGG
                     AGTFSDGKLWSQISDPRHLTRKVIDEFIAAGAPEEIGFVAKPHIGTFRL"
BASE COUNT          747 a         1489 c         1487 g          737 t
ORIGIN      
        1 ccagctcctg gttgacgatc gccacgatgt agtcgccgct ctcggcattg gcatgcacgg
       61 cagccgagac catcacgacg gcggcggcca gagcgcgcca ggagaagcgg aattcagtca
      121 tagggcgtga aaggcgtgtt cgtgtcgcgc tcttcgcgca acagctgata gcccggaata
      181 ttgtccttca acacatgcaa cggattggat ccaagccgcg acagcccgac caactcgagt
      241 tgcagcagca gccgcgtcgt cgcttcgctc aggccggtcg acaggcgctc gacgacgatc
      301 cggccgatcc agcagccagc gtcgtactcg aaaccgacga tcgaatccgt cagacggctg
      361 tcgcgggtgc tgtagttgac ccggccgacg ctgtagagcg tgccgccgca acccccgcca
      421 ccggccggcc ggttgcggtc gctgcctcgg gcccgtccat agaccggcca ttgccaaccg
      481 agttcaacct gttcggcaag gccgcgcgag aggcggtagt tgaagctgac ggtccgaaac
      541 ggacccggcg agtagcgaaa gccggccagc gactgctcga tccggtggct gtcggggttg
      601 tattgggcat cggcatcgaa attccagtgc ggcacgatgg aggtcgagcc gaggaccagg
      661 atgtcggaaa agcgctgcgt gaggggcggc ccgtcgggcg tgaccagctg gtcgcgaaac
      721 agatagcgct gggcgatgcc cagccgcatc gattccgccc cgctctgcgg atcgagaaag
      781 cgcgtcgtga cgccggccgt cagctgatgc gagtctgaga cacgatcgac gcccgaaaac
      841 gagttctcgg tgaagaccga gtcgaaattg aagtccttcg cgaacgagtc gaaattcggc
      901 aggccgtctt ggcggacgta cggcgtatcg acatagaaca gccgcggctc gagcgtctgg
      961 cgaacgtcgc ggccgaacag cgtcgtctcg cgctcgagcg tccaggcgct gtcgatgctg
     1021 agcgtgggta tcacgcgaga ggcgctgcgc cgtccgtcgg caagcggcat gtcgagcgca
     1081 tacgacgcgg cgttgaacga gagcttgggc gtgagcgtcc agcccggtga atagaacggc
     1141 cggctgatgc tgccgagcgc gtccacgcga aggccggtct ggcgcggcag caggtaggtg
     1201 tcgtcgggat tggagaaacg attgaattcg gtctcgaaac caacctcgaa gccgtgtgcc
     1261 caaggtgcgg catagcgcgc gccgatctgc ggcatgcgtt cgtacggcgc ggggtcgatc
     1321 cgcgtcgtcg gatcgaccgt ctgcagcagt tgccagcgct cgacgcgtgc gtaggtcgac
     1381 cagtcaccga acggccgcga cacctgcagg tcggtttgca gcaggcgctg cgtcgtcgtc
     1441 ttcagttcgt cgggaaaatc cttccagtag tcgtcgtcgg agacacgcag cacgtgggcc
     1501 tgcgcatacg ctccgaacgg caacgtgcct tcatgcgcga tgcggagcga atagcgcgaa
     1561 cgcccgaccg tatcgtcgtg gggcaggagc ttgaagtcgg cctcgcccga gtattcgggc
     1621 tcgaggtagc ggaattcggt ctccagaccc ggcccgcgac gcaggctgtc tgatgcagtg
     1681 aaggtggcgt cgcggtttgg cgcgatgttc cagtagtagg gaagcgcgga ctgcacgccg
     1741 ctgcgactgt cgaggccgat gctcggcggc aggaaccccg acttgcgctc ctcgttcagc
     1801 ggaaagctca gtaccggtga cgccaggatg ggaacgccgt agaaacgcaa cacggcgttc
     1861 ttggcgacgc cctcgttgat ctcgttgtcg atgcggatgc tctgcgcctt caggatccag
     1921 gcaggctcct gcgggtcctc gaccgtgcaa ctcgagtacg tcgcatcgaa agcgatggag
     1981 cgctggtcgt cgatgaagtc gatccgggtc gccttgcctc cggcgtgtgt cttcgcgaac
     2041 cggtacgacg gcgactcgaa aaacccttcg aagcgctcga ccttcagttg cagttcgggt
     2101 ccggagaaga cgttgccatc gcggctgatg acgacgtgcc cggaggcccg cgctatgtcg
     2161 tccgtctgat cgtaggtcaa ccgatcggcg cgcacgacga catcgccacg gcgaaactcg
     2221 acgtcgccga ccgcttcggc gtcgaggtcc gggcgtccgt ggatctcgcg gtcacgaaga
     2281 atgatcggca actgccgccc cgcgtcgccg cgcggcaagg gctgcaacgc ggccgcctgg
     2341 cgaagctgga tccccgaggc cgccgatgcc ggcgccgaag gtgccggagc gcttgcaggc
     2401 tcgctttccg gcaccgctgc agtttgcgcc caggcagcgg cagtgaacgt cgcgatggcg
     2461 accagcacca cgacgatcca tgctgccgac ttcgtgccgg ccctggcgag cgccgacacc
     2521 gaagccagcg gcccgacccg gcccagtgac attcgagact cttggctgaa ggaatggcgc
     2581 acgaggcgga cggcagcaga acgcgggctg aagcgccggc acggcgcgaa gccggcgttc
     2641 gcgagccgcg gtgccgggcg gacgatgccg gcctcggtct agcggcatcg atttgtagaa
     2701 tcgattatcc acgaggcacg cgcgtccgca cctcgcagca acgtttccgc cccgtgattc
     2761 ctccttcctc gccaggccgc atcgcctggg ccgatccggc ccgcgaagcg gcatttctcg
     2821 actggctcgg atgcgtcgcg tcgcgccacg acctggtcgt cgccacgctt cgccaagcgt
     2881 ccagcgatgc gagctttcgc cgctatctgc gcgtcgaccg cgccgacggc ggcagcatgg
     2941 tcgtcatgga tgcgccgccg cctgaagaaa acgtgcgacc cttccttcac gtcgccggcc
     3001 tgatcgagcg cgccggcctg cacgcgccgc acgtgttcga agccgacccg gagcatggct
     3061 tcgtcctgct cgaagacctc ggcggcaccc tgtatctcga ggcgctgctg gccacgcagc
     3121 aggacgacga tccgcaacgc gcggactcgc tgatgcaggt cgccatccgc gcgctcgtcg
     3181 cctggcaacg cgatctgccg gcaacgaccc tgcctgccta cgacgacgca ctgctgcgac
     3241 gcgaactggc aatctttccc gagtggtgcg tctgccgcga gcatcgggtc acgtggaccg
     3301 acgcagaagc ggagacctgg gctcgcgcct gcaatctgct cgtcgccagc gcactcgcgc
     3361 agccggtcgt cacggtgcat cgcgactgga tgccgcgcaa cctgatggtg agcgacccca
     3421 acccgggcat tctcgacttc caggatgcgg ttcgcggtcc gattgcctac gacgtcgctt
     3481 cgctgcttcg cgacgccttc gtgtcgtggg acgaggagcg cgaaatcgac tgggcgatcc
     3541 gctactggga gggtgcgcgc aaggccggac tccccgtgcg cgacgatttc ggtgaattct
     3601 ggcgcgagct cgagtggatc ggactgcagc ggcacctcaa ggtgctcggc atcttctgtc
     3661 gtctcaagca ccgagacgac aagcccgcct acagcgccga cctgccgcgc ttcttcgcgt
     3721 acgcgcaccg cgtctcgacg cggtattcgg cgctgcggcc gctgtcccga ttgctcgagc
     3781 cgctgatggg cgtctctcgc atcgaggctt tccattagcc ttccgagcgg accatgcttc
     3841 gccttaccga gctgcgcctg ccgctcgagc acacgcaggc cgagctccgc gctgccgtgc
     3901 tcggccggct cggcatcgac gagtcgtcgc tcggatcatg gagagtcttt cgccgcggct
     3961 acgacgcgcg aaagcgcggc gcgatcgtcc tcgtctacac gatcgactgc gagatcgaag
     4021 acgacgctgc cgtcctcggt gcgcatgccg gcgatccgaa tctgcgaccg gcgcccgaca
     4081 cgacctaccg attcgttgcg cactccgact cgcgccagcg cgggccgcgg ccagtggtcg
     4141 tcggcttcgg accctgcggc ctgttcgcgg cgctcatcct ggcgcagtcc ggctttcgtc
     4201 cgctcgtcat cgaacgcggc aaggccgtgc gcgagcgcac ccgcgacacg tggggcctgt
     4261 ggcggcgcgg cgtcctcgac ccggagtcga acgtccagtt cggcgaaggc ggcgccggta
     4321 ccttctccga cggcaagctg tggagccaga tcagcgaccc acgccacctg acgcgcaagg
     4381 tcatcgacga attcatcgcc gccggagcgc ccgaggagat cggcttcgtc gccaagccgc
     4441 acatcggcac cttccgcctg
//
LOCUS       DASRWS010000300         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_992078, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000300 DASRWS010000000
VERSION     DASRWS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992078"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFF72_05615"
     CDS             complement(<1..1178)
                     /locus_tag="VFF72_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZV92674.1"
                     /translation="MSPLELTALIFFLMLALMALRVPIAISMFIAGSIGYVTQAGWAP
                     YSNFLNEEAFARFASYDLSVIPLFILMGHFASQGGISKALFAFASVVMGRFRGGLAMA
                     AVLASGAFGAICGSSIATAATITTVALPEMRRHGYSGRLATGTLAAGGTLGILIPPSV
                     PLIVYAILAEQNIAKLFAAATIPGLLAMIGYLIAISIYVRLFPGHAPQADQVPGRLTW
                     RLVVGVLPIGVIFAVVFGGIYGGLFSPTEGAAVGAASTFVAAILRREIDWAKFKHCFY
                     ATAQASGMIFLIFIGADMMNSALALTQVPMQLAEVVKAMGLPPLLVVTAILLLYMALG
                     CIMDELSILLLTIPIFFPLVIGLDFGMPRESVAIWFGIMVLVTVGFGLLSPPVGLNVY
                     V"
     gene            complement(1228..1701)
                     /locus_tag="VFF72_05620"
     CDS             complement(1228..1701)
                     /locus_tag="VFF72_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZV92675.1"
                     /translation="MRTLEMLAKLCAVAAGLLLTAVTLMTCGSLIGRNLGGMTIVGDF
                     ELSGAAAGAAVALFMPWCQIERGNIIVDFFTVRASEATRQRLDRFGALLLGLAMALLA
                     WRTTLGALSSYRAGSGTMLMNFPNWVIYTAMVPPLVLTALIGLAQSWRGFPVVKE"
     gene            complement(1759..2094)
                     /locus_tag="VFF72_05625"
     CDS             complement(1759..2094)
                     /locus_tag="VFF72_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV92676.1"
                     /translation="MDADGVEVFLVEPDGLRHRVEARIGQSLMRAATDAGIDAIKADC
                     GGLMTCATCHVYVEPDWAERLPPPTSDEDAMLEMTAAPRRATSRLSCQIVLSRELAGL
                     TAGLPETQY"
     gene            complement(2102..3586)
                     /locus_tag="VFF72_05630"
     CDS             complement(2102..3586)
                     /locus_tag="VFF72_05630"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZV92677.1"
                     /translation="MTTFAEACGAAGPARMAAAARMLGRMRDEGIERVRIGWCDLHGA
                     LRGKTLMPESVPRAIDAGVGMVSTILLKDSSDRTAYRVFEPGVTTALPGFGFANNVVL
                     LPDPASFQSLPWAPGTGWLRAEAWFEDATPVPIDTRQVLRAALDRLAAAGFGLKCGLE
                     VEFHIHRLVDGDADASLDPDRAGWPGQTPAVRMIHPGYNLLAESATDMADDALRIVQH
                     TAQGLGLPLASLEIELGPSQVEAVFDATDALTAADQMVAFRNGVRQALRREGFHASFV
                     CVPPFAEAMASGWHLHQSLVDLRSGENAFRRTVPGVDCGPLDAASTLSDAGAGWLAGL
                     LAHGRAMAVFCAPTINAYARFRPNAMAPNAVIWGRDHRGAMLRVLGRCGDPATRIENR
                     IGEPAANPYLYLAAQIHAGLDGIERGLRPPPATGAPYADEHERLPASLGEALDALAAD
                     APLCKAFGSTVVDWLSRVKRFEIARHDAADDKRSWEAREYFGRL"
     gene            complement(3583..4971)
                     /locus_tag="VFF72_05635"
     CDS             complement(3583..4971)
                     /locus_tag="VFF72_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZV92678.1"
                     /translation="MNAEQNERLTRIGPGTACGALMRSYWQPAALLDEFDPRLDPSMA
                     VRPMKAVRLLGEDLVMFRDGDGRFGLIERACPHRGADLAFGRLEPEGLRCPFHGWKFT
                     VDGRCLETPAEPAGSTLCQRIRQRSYPVMERSGVLFAWMGPEGSTPPALPAFDAFIAP
                     ASHSFAFKGLWHCNWLQAFEVGIDPAHPSFLHRYLEDESLDDAYGRQFRSASVGEVGG
                     ERWPMTRVMREFSQPEIRFDNVAEGLVRLTTLRPMTEALTHVRVTHAAFPTTFVIPLS
                     ETITITQMHVPVDDTHTYWYSFFTSFAEPLDKEAMRSQRLAHVSLPDYAPRNGRHNRW
                     GFDAEEQRTRTYLGMGEEDINRHDQWAVESMGAIQDRTREHLGTTDKVIMANRRTLLK
                     AIETVAQGGRPPLAVDADAAAALQGPDTIDCIAPAGSWAEFWSNGAAAKRRAAGWLAA
                     AVPRADLEGAHP"
     gene            5153..>5438
                     /locus_tag="VFF72_05640"
     CDS             5153..>5438
                     /locus_tag="VFF72_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV92679.1"
                     /translation="MQAATQPVLARFSAGLAALPKHARLREAIVGAVQAGELPAGTKM
                     AGERELSAALGLSLGTTQKALGRLVDEGFLVRRQGHGTFVGTSRRAVAGSW"
BASE COUNT          888 a         1846 c         1939 g          765 t
ORIGIN      
        1 acgtagacgt tcaggcccac cggcggcgag agcaggccga agccgaccgt caccaggacc
       61 atgatcccga accagatcgc caccgattcc cggggcatgc cgaagtcgag gccgatgacg
      121 agcggaaaga agatcgggat cgtcagcagc aggatcgaga gctcgtccat gatgcagccg
      181 agcgccatgt agagcagcag gattgccgtc acgacgagca gcggcggcag gcccatcgcc
      241 ttgacgacct cggccagctg catcggcacc tgcgtcaggg ccagggcgga attcatcatg
      301 tcggcgccga tgaagatcag aaagatcatc cccgaggcct gcgccgtcgc gtagaagcag
      361 tgcttgaact tcgcccagtc gatttcccgc cggaggatcg cggcgacgaa ggtcgatgcg
      421 gcgccgaccg cggcgccttc ggtcggcgag aagaggccac cgtagatgcc gccgaagacg
      481 accgcgaaga tgacgccgat gggcagcacg ccgacgacga gccgccaggt gagccgtccc
      541 ggcacctggt cggcctgcgg ggcgtggccc gggaagaggc ggacgtagat cgagatggcg
      601 atcaggtagc cgatcatcgc caggagtccc ggtatcgtcg cggcagcgaa cagcttggcg
      661 atgttctgct cggcgaggat cgcgtagacg atcagcggca ccgaaggcgg aatgaggatg
      721 cccagggtgc cgccggcggc cagcgtgccg gtcgcgagcc gacccgaata gccgtggcgc
      781 cgcatctcgg gcagcgcgac ggtcgtgatc gtcgccgccg tcgcgatcga tgagccgcag
      841 atcgcgccga acgcccccga agccagcacc gcggccatcg ccaggccgcc gcgaaaacgc
      901 cccatgacga cgctcgcgaa cgcgaagagg gccttcgaga tgccgccctg cgaagcgaag
      961 tggcccatca ggatgaaaag cggaatgacc gagaggtcgt agctcgcgaa gcgcgcgaac
     1021 gcctcctcgt tgaggaagtt cgaatacggc gcccagccgg cctgcgtgac gtagccgatc
     1081 gagccggcga tgaacatgga gatggcgatc ggcacgcgca gcgccatcag cgccagcatg
     1141 agaaagaaga tgagcgccgt gagctctagc ggggacatga agcccccacg cttgccgcca
     1201 tcgcggccgc gctgccgacc acggccctca ctccttcacc accggaaagc cgcgccacga
     1261 ttgggcgagg ccgatcagcg cggtgaggac gagcggcggc accatggccg tgtagatgac
     1321 ccagttcgga aagttcatga gcatcgttcc cgaaccggcg cgatacgagc tgagggcgcc
     1381 cagcgtggtg cgccacgcga gcagcgccat ggcgaggccg aggagcagcg cgccgaaacg
     1441 gtcgaggcgc tggcgcgtcg cctcgctggc gcgcacggtg aagaagtcga cgatgatgtt
     1501 gccgcgctcg atctggcacc acggcatgaa gagcgccacg gcggcgccgg cggcggcacc
     1561 cgacagctcg aagtcgccga cgatcgtcat gccgccgagg ttgcggccga tcaggctgcc
     1621 gcaggtcatc agcgtcaccg cggtaaggag caggcccgcc gcgacagcgc aaagcttggc
     1681 gagcatctcg agcgttcgca aggcgtttcc cgggtcgacc ggaagctgtg tggatggggc
     1741 aagtatcgag gctgcgcctc agtattgcgt ctcgggtaag cccgccgtca atcccgcgag
     1801 ttcccggctc agcacgatct ggcagctgag gcggctggtc gcccggcgcg gcgccgccgt
     1861 catctcgagc atcgcgtcct cgtccgaggt cggcggcggc aaccgttcgg cccagtctgg
     1921 ctcgacgtag acgtggcagg tggcacacgt catgagaccg ccgcaatcgg ccttgatggc
     1981 gtcgatgccg gcgtcggtcg cggcgcgcat caggctttgg ccgatgcgag cctcgacgcg
     2041 gtgacgcagg ccgtcgggct cgaccaggaa gacctcgacc ccatcggcat ccatgcgcgt
     2101 ctcagaggcg gccgaagtac tcgcgcgctt cccaagaccg cttgtcgtcg gccgcgtcgt
     2161 gacgcgcgat ctcgaagcgc ttgacgcggc tcagccagtc gacgaccgtc gagccgaagg
     2221 ctttgcaaag aggcgcgtcg gcggccagcg cgtcgagcgc ttcgcccagg ctcgccggca
     2281 ggcgctcgtg ctcgtcggcg taaggcgcgc cggtggccgg cggcggccgc aggccgcgct
     2341 cgatgccgtc gagaccggcg tggatctgcg ccgccaggta gaggtacgga ttggcggccg
     2401 gctcgccgat gcggttctcg atccgcgtcg ccggatcgcc gcaacggccg agcacgcgca
     2461 gcatcgcgcc gcggtgatcg cgtccccaga tgaccgcatt gggcgccatc gcgttgggac
     2521 ggaagcgcgc atacgcattg atcgtcggcg cgcagaagac ggccatggcg cggccgtgcg
     2581 cgagcagccc ggcaagccag cccgcgccgg cgtcggagag cgtgctcgcc gcatccaggg
     2641 gcccgcagtc gacgccgggc actgtgcggc gaaacgcgtt ctcgccactg cgcaggtcga
     2701 cgagcgactg atgcaggtgc cagccgctgg ccatcgcctc ggcgaacggc ggcacgcaga
     2761 caaagctcgc gtggaatccc tcgcgtcgca gcgcctggcg aacgccgttg cgaaaggcga
     2821 ccatctgatc ggccgcggtc agtgcatcgg tcgcgtcgaa caccgcctcg acctggctcg
     2881 ggccgagctc gatctcgagc gaggcgagcg gcaggccgag gccctgggcg gtgtgctgga
     2941 cgatgcgcaa cgcgtcgtcg gccatgtcgg tcgcgctctc ggcgagcagg ttgtagcccg
     3001 gatggatcat gcgcacggcc ggcgtctggc ccggccagcc ggcccgatcc gggtcgagcg
     3061 aggcatcggc gtcgccgtcg acgagccgat ggatgtggaa ttcgacctcg agcccgcact
     3121 tcaatccgaa gccggcggcg gccagacggt cgagcgccgc gcgcaggacc tggcgcgtgt
     3181 cgatgggcac cggcgtcgcg tcttcgaacc acgcctcggc gcgcagccag ccggtgcccg
     3241 gcgcccatgg caggctttga aagctcgccg gatcgggcag cagcaccacg ttgttggcaa
     3301 agccgaatcc gggcagcgcc gtcgtcactc ccggctcgaa gacgcgatag gcggtgcggt
     3361 cggagctgtc cttcagcaag atggtgctga ccatgccgac gccggcatcg atggcgcgcg
     3421 gcacgctttc gggcatcagc gtcttgccac gcaaggcgcc gtgcaggtcg caccagccga
     3481 tccgcacccg ctcgatgcct tcgtcgcgca tccgcccgag catccgcgcg gcggccgcca
     3541 tgcgcgccgg tccggcagcg ccgcacgcct cggcgaaggt cgtcaagggt gcgcgccctc
     3601 gaggtcggcg cgtggtacgg cagcggcgag ccagccggcg gcacgccgct tcgccgccgc
     3661 gccgttcgac cagaactcgg cccacgaccc ggccggagcg atgcagtcga tcgtgtccgg
     3721 accctgcagc gccgcggcgg cgtcggcatc gacggcgagc ggcggccggc cgccctgcgc
     3781 caccgtctcg atcgccttca gcaacgtacg tcggttggcc atgatcacct tgtcggtggt
     3841 gcccagatgc tcgcgggtgc ggtcctggat ggcgcccatg ctctcgaccg cccactggtc
     3901 gtggcgattg atgtcttcct cgcccatgcc gagataggtg cgcgtgcgtt gctcctcggc
     3961 atcgaagccc cagcggttgt ggcggccgtt gcgcggcgcg tagtcgggca gcgagacgtg
     4021 ggccagccgc tggctgcgca tcgcctcctt gtcaagcggc tcggcgaagc tcgtgaagaa
     4081 gctgtaccag taggtgtggg tgtcgtcgac cggcacgtgc atctgcgtga tcgtgatggt
     4141 ttccgagagc ggaatgacga aggtcgtcgg gaaggccgcg tgcgtgaccc gcacgtgggt
     4201 gagcgcctcg gtcatcggcc tgagcgtggt cagccggacc aggccttcgg cgacgttgtc
     4261 gaagcggatc tcgggctggc tgaactcgcg catcaccctc gtcatcggcc agcgctcgcc
     4321 gccgacttcg ccgacgctgg cgctgcggaa ttgccggcca taggcgtcgt cgagcgattc
     4381 gtcctcgaga tagcggtgca ggaacgacgg atgcgccgga tcgatgccga cctcgaaggc
     4441 ttgcagccag ttgcaatgcc acaacccctt gaaggcgaag ctgtggctcg ccggcgcaat
     4501 gaaggcgtcg aacgccggca gcgccggcgg cgtcgatccc tccggcccca tccacgcgaa
     4561 gagaacgccc gagcgttcca tcaccggata gctgcgctgg cggatgcgct ggcaaagcgt
     4621 cgaccccgcg ggctcggccg gcgtctcgag gcaacggccg tcgaccgtga acttccagcc
     4681 gtggaacggg cagcgcaagc cttcgggctc gagccggccg aaggccaggt cggcgccgcg
     4741 atgcgggcac gcccgctcga tgaggccgaa gcggccgtcg ccgtcgcgaa acatgacgag
     4801 gtcctcgccg agaagtcgca cggccttcat gggccgcacc gccatcgacg ggtcgaggcg
     4861 cggatcgaac tcgtcgagca gcgccgccgg ttgccagtag ctgcgcatca gcgccccgca
     4921 ggccgtgccc gggccgatgc gggtgagacg ttcgttctgc tcggcgttca tcgctggatc
     4981 ctttcggcgg cagtctacag gcggggatcg gcctggagcg tacgggccgc gacggcctgc
     5041 agcgcactcc ccgttgcatc gggcgaaggt caggcacacg gaacacaagc tccggtgctc
     5101 tagagctttc gtgctcttga ccccgtcatg gcggctgcct agactccgcg tgatgcaggc
     5161 cgccacccag ccggttctcg cccgcttcag cgccggtctt gccgcgttgc ccaagcacgc
     5221 ccggctgcgc gaagccatcg tcggcgcggt gcaggccggc gaactgcccg ccggcacgaa
     5281 gatggccggc gagcgcgagc tgagcgcagc gctcgggctc agcctcggaa cgacgcaaaa
     5341 ggccctcggc cgacttgtcg acgagggatt cctggttcgc cgtcaggggc acggcacgtt
     5401 cgtcggcacg agccggcgcg cggtcgcggg ttcctggc
//
LOCUS       DASRWS010000301         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4156477, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000301 DASRWS010000000
VERSION     DASRWS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4156477"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..874
                     /locus_tag="VFF72_05645"
     CDS             239..874
                     /locus_tag="VFF72_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92680.1"
                     /translation="MNTLTLDATFGSVVVLVAALLYGLVCAVVAWRFTTARRARARSA
                     PAVAEAVRFAARDGRVRIDAWHLPAKAARGAIVFVHGKDACRGDELKSPTFALARAFV
                     AAGLSVLMIDLRGHGSSGGARLTYGANERFDVLAPSIGCAATATSASACWARRWVRRH
                     RSSLPPTSRASPRSSPTARSSTSARWSNASTAACRTCRPAFFPVHWRWAGC"
     gene            937..1173
                     /locus_tag="VFF72_05650"
     CDS             937..1173
                     /locus_tag="VFF72_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92681.1"
                     /translation="MLVIHSSRDHFIPVADAWAIAGSAGADLWVTDSDGHLGSYRADP
                     IAYTERVVTFFGRHLAAPEAAQTGVSTTPLALAA"
     gene            complement(1230..1640)
                     /locus_tag="VFF72_05655"
     CDS             complement(1230..1640)
                     /locus_tag="VFF72_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZV92682.1"
                     /translation="MNTTEPFKRSPCAIANSLDIVGDKWSLLVVRDLLHGKRTYGELA
                     NSRERIPTNILAERLKRLQAAGIVVGTAYQQHPVRYAYALTPKGKALGDVLLAFVRWG
                     KQHIPGTMAIKQEPARASSAGSPATKHRARTSRE"
     gene            1839..2495
                     /locus_tag="VFF72_05660"
     CDS             1839..2495
                     /locus_tag="VFF72_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92683.1"
                     /translation="MSATHLLLPAASQPAGGNRIRLAVGLVLAAWFLLVVSLGAAGAF
                     VGPPRTPPVAIAVGFAAPLLLFLAWLRLSPSFRDFVLSLDLRLIAAIQAWRFAGMELL
                     ALYAHGVLPAMFAFPAGLGDMAVGLAAPWMVLALVRQPEFAVRPAFIRWNVLGILDLV
                     VAIGLGALGAALATGAPGETSTAPMATLPLLLIPAFLVPLFLMLHAAALMQSRRMARR
                     "
     gene            2648..>3080
                     /locus_tag="VFF72_05665"
     CDS             2648..>3080
                     /locus_tag="VFF72_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92684.1"
                     /translation="MNPDLPRRNLLKAGTASLGGAVMGLQAAAARAQGASTEAGTTVV
                     AVPPLAKASGLRLATCDFGDGGPARIAIVLDGGHVVDVAAQARRVGMKLSFPADSMLA
                     MLASGDGAMAQMRSLAAMVAGGRVATLPMRQVDFHSPIPKPQ"
BASE COUNT          385 a         1081 c         1074 g          540 t
ORIGIN      
        1 gcgacgcgtc gcaggttcac ggaactggcg gtgagggcgg cggcagttgt catggcggcg
       61 gtggcctgga gcggtgccgc catcgtctgc agccgcgttg ccggcgtcct gaagccgccc
      121 tgaaacgttt ctcaaaccgc gccggtcgcg tctttcagaa aggcttgaga cgggcttcag
      181 gacgcgaggg tggccgcacg agacagtggc gactcgtcct tcacccactg gaatcgccat
      241 gaacacgctc actctcgatg cgaccttcgg cagcgtcgtc gtcctcgtcg ccgcgctgct
      301 ctacggcctg gtctgtgccg tcgtggcgtg gcgtttcacc accgcgcgcc gggcgagggc
      361 gcggtcggcg ccggcggtgg ccgaggcggt gcgcttcgct gcgcgcgacg gccgcgtccg
      421 gatcgacgcg tggcatctgc cggcgaaggc ggcgcgcggt gccatcgtct tcgtgcacgg
      481 caaggacgcc tgtcgtggcg acgagctgaa gtcgccgacc ttcgcgctcg cccgggcctt
      541 cgtcgccgcg gggctttcgg tgctgatgat cgatctgcgc ggccacggca gcagcggcgg
      601 ggcgcggctg acctacggcg cgaacgaacg cttcgacgtg ctggcgccgt cgattggctg
      661 cgcggccacg gccaccagcg catcggcctg ctgggcgcgt cgatgggtgc ggcgacatcg
      721 atcttcgctg ccgccgacga gccgggcgtc gccgcggtcg tcgccgacag cccgttcgtc
      781 gacttcggcg cgatggtcga acgccagtac cgccgcctgt cgcacctgcc gacctgcttt
      841 cttcccggtg cactggcgct gggccggctg ctgaccggcg tcgacctgcc gcgagtgcgg
      901 ccgctggagg cggcgcgtgc gctgtccggt cgtccgatgc tggtgatcca cagcagccgc
      961 gaccatttca tccccgtggc cgacgcctgg gccattgccg gcagcgccgg agccgacctc
     1021 tgggtgaccg acagcgacgg ccacctgggc tcgtaccgcg ccgaccccat tgcctacacc
     1081 gagcgcgtcg tcaccttctt cggccggcac ctggcggcgc ccgaggcggc gcagacaggc
     1141 gtgtcgacga cgccgcttgc gctggcggcg tgagcgacgg cggtacccgg atgctccccg
     1201 acacgtccgt catttcctcc cggtgaccat cactcgcggc tcgtccgcgc gcgatgcttg
     1261 gtcgccggcg accctgccga cgaggcacgc gccggctcct gcttgatcgc catcgtgccg
     1321 ggtatgtgct gcttgcccca gcgcacgaac gcgagcagca cgtcgcccaa cgccttgccc
     1381 ttcggagtca gggcgtaggc gtatcgcacc ggatgctgtt gataggcggt gccgacgacg
     1441 atccctgcgg cttgcaggcg cttgaggcgc tcggcaagga tgttggtcgg aatgcgctcc
     1501 cgcgagttcg cgagttcccc gtaggtgcgc ttgccgtgca gcaagtcgcg caccacgagc
     1561 agggaccact tgtcgccgac gatgtcgagg gaattggcga tcgcgcacgg ggagcgcttg
     1621 aaaggctcgg tggtattcat cctgggcagt tttccatggg gtcgccgatc cagtgtgcgg
     1681 cacggcccga tcggcagcgc gtcggcgtcg cgacgcaggg atctgggcgg ccggcgtttc
     1741 gcaattctct gctccgtcag ctggtgactt gcatgatgca agtgagtatg ctacgttccc
     1801 tgtaacttgc aaaatgcaag tcactaagga gagcattcat gtcagccaca caccttctcc
     1861 tcccggcggc gtcgcagccc gccggcggca accgcattcg cctggccgtc ggcctcgttc
     1921 tcgccgcctg gttcctgctc gtcgtgtcgc tcggtgcggc aggcgctttc gtcggccccc
     1981 cgcgaacgcc gccggttgcc attgccgtcg ggttcgcggc gcccttgctg ctgtttcttg
     2041 catggctgcg cctctcgccg tcatttcgcg atttcgttct ctcgctcgat ctgcgtctga
     2101 tcgcggcgat tcaggcgtgg cgcttcgccg gaatggaact tctcgcgctg tacgcgcacg
     2161 gcgtcctgcc agccatgttc gcattcccgg ccggcctggg cgacatggcc gtaggcctgg
     2221 cggcgccgtg gatggtgctt gccctggtgc gccaacccga gttcgctgtc cggccggcgt
     2281 tcatccgctg gaatgtgctg ggcatcctcg acctcgtcgt ggccatcggc ctcggcgctc
     2341 tcggtgcggc gctggccacg ggcgccccgg gtgagaccag cacggcgccg atggcgacac
     2401 tgccgctgct gctgatcccg gcgttcctcg tgccgttgtt cctgatgctg cacgcggcag
     2461 cgttgatgca gagccggcga atggcccgac gctgaggagg gtgggcgggc agacgcgtgg
     2521 ccaacggcgg caccgcgccg gagccgcatc gccatcacgg catcgccgcg tcgaaacgtg
     2581 tgcgcgaatg cgggttcggc cgaagcctag actctcggct gatcccctca cgacacccgg
     2641 agccaccatg aaccctgacc tgccacgccg caacctgctc aaggccggca ccgcctcgct
     2701 cggcggcgct gtgatgggcc tgcaggccgc agcggcacgc gcccagggcg catcgaccga
     2761 agccggcacc acggtcgtcg ccgtgccgcc gctggccaag gcgtccgggt tgcgcctggc
     2821 gacctgcgac ttcggcgacg gcggcccggc gcgtatcgcc atcgtcctcg acggcggcca
     2881 tgtcgtcgac gtcgcagccc aggccaggcg cgtcggcatg aagctgagct tccccgccga
     2941 ttcgatgctc gccatgctgg ccagcggcga cggcgccatg gcgcagatgc gttcgctggc
     3001 ggcgatggtc gcgggcgggc gggtcgcgac gttgccgatg cggcaggtcg acttccattc
     3061 gccgatcccg aagccgcagg
//
LOCUS       DASRWS010000302         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_992342, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000302 DASRWS010000000
VERSION     DASRWS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992342"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2132)
                     /gene="mfd"
                     /locus_tag="VFF72_05670"
     CDS             complement(<1..>2132)
                     /gene="mfd"
                     /locus_tag="VFF72_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZV92685.1"
                     /translation="DDAGAAPWATALPDLSVDRGAPEPLSRLQRHVEATPNRVLIVAE
                     SEGRRETLLELLRDSRIDPPATASLAEFEVGSERFAIAVAPLAEGFAWLRPGATAGIE
                     FVTETELFATAPGTRRRRRQEQTSDVTALIKDLSELKVGDPIVHVNHGIGRYHGLTTI
                     ALGGPDEEPSEFLHLEYAEKATLYVPVAQLHLIARYTGVSAEEAPLHKLGSGQWEKAR
                     RKAAEQVRDTAAELLALYARRAAREGFAFRFEAHDYEAFAASFGFEETPDQHAAIHAV
                     IQDMVSPRPMDRLVCGDVGFGKTEVALRAAFVAVSGGRQVAILAPTTLLAEQHFHNLA
                     DRFGKWPIKVAELSRFRSPKEVKAALAGLAEGTIDIVVGTHKLLSADVKFARLGLLVI
                     DEEHRFGVRHKEQIKAMRAEVDVLTLTATPIPRTLGMALEGLRDLSVIATAPQRRLAI
                     KTFVRNEASGTIREAVLRELKRGGQMYFLHNDVATIEHRRETLQALVPEARIAVAHGQ
                     MPERELERVMRDFVAQRFNVLLCSTIIETGIDVPSANTIVISRADKFGLAQLHQLRGR
                     VGRSHHQAYAYLLVPDVEGLTRQAAQRLEAIQQMEELGSGFYLAMHDLEIRGAGEVLG
                     ESQSGNILEVGFQLYNDMLAEAVRALRNGREPDLAGPLAATTEINLHAPALLPDAYCG
                     DVHTRLSLYKRLASAEKAEQIDALLEEI"
BASE COUNT          287 a          714 c          777 g          354 t
ORIGIN      
        1 tgatctcttc gagcaaggca tcgatctgct cggccttttc cgcgctcgca agccgcttgt
       61 agagcgacag ccgcgtgtgc acgtcgccgc agtaggcgtc gggcagcagc gcgggagcgt
      121 gcaggttgat ctcggtcgtc gccgccagcg ggccggcgag atccggctcg cggccgtttc
      181 tcagcgcgcg cacggcctcg gccagcatgt cgttgtagag ctggaagccg acttccagga
      241 tgttgccgct ctggctctcg ccgagcacct cgccggcacc gcggatctcg aggtcgtgca
      301 tggcgaggta gaagcccgag cccagctctt ccatctgctg gatcgcctcg agacgctggg
      361 ccgcctgcct ggtcagtccc tcgacgtcgg ggacgagcag gtaggcatag gcctggtgat
      421 gcgagcggcc gacacggccg cgcagttggt gcagctgggc cagcccgaac ttgtcggcgc
      481 gcgagatgac gatggtgttg gcgctcggca cgtcgatgcc ggtctcgatg atcgtcgagc
      541 acagcagtac gttgaagcgc tgcgccacga agtcgcgcat cacccgctcg agctcgcgct
      601 ccggcatctg gccgtgggcg acggcgatgc gtgcctcggg gacgagcgcc tgcagcgtct
      661 cgcggcgatg ctcgatcgtc gccacgtcgt tgtgcaggaa gtacatctgg ccgccacgct
      721 tgagctcgcg cagcaccgcc tcgcggatcg ttcccgacgc ctcgttgcgc acgaaggtct
      781 tgatcgccag gcgacgctgc ggcgcggtgg cgatcacgga gaggtcgcgc agtccttcga
      841 gcgccatgcc gagggtgcgc gggatcggcg tcgccgtcag cgtgagcacg tcgacctcgg
      901 cgcgcatcgc cttgatctgc tccttgtggc gaacgccgaa gcggtgctcc tcgtcgatga
      961 cgagcaggcc cagccgggcg aacttcacgt cggccgagag cagcttgtgc gtgccgacga
     1021 cgatgtcgat cgtgccttca gcgaggccgg cgagggccgc cttcacttcc ttgggcgagc
     1081 ggaagcgcga cagctcggcg accttgatcg gccacttgcc gaagcggtcg gcgaggttgt
     1141 ggaagtgctg ctcggcgagc agcgtcgtcg gcgccaggat ggcgacctgc ctgccgccgc
     1201 tgacggcgac gaaggcggca cgcagggcga cttcggtctt gccgaagccg acgtcgccgc
     1261 agaccaggcg atccatcggc cgcggcgaga ccatgtcctg gatgaccgcg tggatggcgg
     1321 cgtgctggtc gggcgtttcc tcgaagccga agctggctgc gaaggcctcg tagtcgtgcg
     1381 cctcgaagcg gaacgcgaag ccttcgcgcg cggcgcgtcg ggcatacagg gcgagcagct
     1441 cggccgcggt gtcgcgcacc tgctcggcgg ccttgcggcg ggccttttcc cactggccgg
     1501 agccgagctt gtgcagcggc gcctcctcgg cgctgacgcc ggtatagcgg gcgatcaggt
     1561 ggagctgcgc caccggcacg tagagcgtcg ccttctcggc gtattcgaga tgcaggaatt
     1621 cgctcggctc ttcgtccggc ccgccgaggg cgatcgtcgt caggccgtgg tagcggccga
     1681 tgccgtggtt gacgtggacg atcggatcgc ccaccttcag ctccgaaagg tccttgatca
     1741 gcgcggtgac gtcgctcgtc tgctcctggc gccggcgccg tctcgttccg ggcgccgtcg
     1801 cgaagagctc ggtctcggtg acgaactcga tccccgcggt cgcgccgggt cgcagccacg
     1861 cgaagccctc ggccagcggc gccaccgcga tcgcgaagcg ctcgctgccg acctcgaatt
     1921 cggcgagcga ggccgtggcg ggcggatcga tgcggctgtc gcgcagcagc tcgagaaggg
     1981 tctcccgccg gccttcgctt tcggcgacga tgaggacgcg attcggcgtc gcctcgacgt
     2041 ggcgctgcag gcggctcagc ggctcgggtg cgccgcggtc gacgctcagg tcgggcagcg
     2101 ccgtcgccca gggcgccgcg ccggcgtcgt cg
//
LOCUS       DASRWS010000303         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2703758, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000303 DASRWS010000000
VERSION     DASRWS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703758"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VFF72_05675"
     CDS             complement(<1..1204)
                     /locus_tag="VFF72_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HZV92686.1"
                     /translation="MDVEDIEDLAGPANALAPPPTPVQTTLGRTSAASLAAATLAACG
                     GGSDAGSNDGGGTGTGGSGSSPPPTEAEASRFLAQASMGANRAQIARVQAIGYSAWLD
                     EQIAMAATPTRWDALVAAGYADPSYKNTEAGFDSVTWQKLLSSPDTLRQRITLAFSEI
                     FVVGIDGLIGSGWKAFAAAGYLDLLEANVFGNHRALLQQISSNPAMGMWLTFRGSMKA
                     NPASGSLPDENYAREVMQLFTIGLVELNLDGTPQLTGGATTYTYGQSDVTGLARVFTG
                     WDFDLNGLSAAAATATPDFVRRPMISYPSRYETGAKTFLGTTIPAGTDATTGLSMALD
                     TIFAHPNVGPFFSRQMIQRLVTSNPTPGYVARVATVFNDDGSGTRGNLGAVIKAILLD
                     DEARSATVA"
     gene            complement(1407..>2519)
                     /locus_tag="VFF72_05680"
     CDS             complement(1407..>2519)
                     /locus_tag="VFF72_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZV92687.1"
                     /translation="VDHLPAWLDGLARALGAAPGGAEAVQAADRETRNAEAHGLQRWQ
                     QGYDLHEVTREWAALHRCLVDEIESYLATRSPPSRRLAAQAHATLAFFIGEATSESAC
                     QYFRLDRLEAAGALHDLEKALADVQETDRRRGELWQQAAHDLRGNLGVVANVAHGLSI
                     NDLPVQRRDDFVGLLRHNVKALHGLLDDVTELARLQAGQETRRVASFDAGELLQRLGD
                     DVRPLAGERNLSLDVRGPRPLAVEGDAVKVRRIAQNLVLNALKYTSQGGVELTWGEGD
                     VADGRWWFEVRDSGPGFHAGPGAPLVSALGAVPGEAPAAADARPVNQAQGEGLGLAIV
                     KRLCELLGAQVELTTATGRGTVVRVLVPRNYAGSSN"
BASE COUNT          376 a          869 c          892 g          382 t
ORIGIN      
        1 cggcgaccgt cgcgctgcgc gcttcgtcgt cgagaaggat cgccttgatg accgcgccga
       61 ggttgccgcg cgtgccgctg ccgtcgtcgt tgaagacggt ggcgacgcgc gccacgtagc
      121 cgggcgtcgg gttgctggtg acgagcctct ggatcatctg cctggaaaag aaggggccga
      181 cgttcgggtg cgcgaagatg gtgtcgagtg ccatcgacag accggtcgtc gcgtcggtgc
      241 cggccgggat ggtcgtcccg agaaaggtct ttgcgccggt ctcgtagcgc gacggatacg
      301 agatcatcgg ccggcgcacg aagtcgggcg ttgccgtggc cgcggcggcg ctcaggccgt
      361 tcaggtcgaa gtcccagccg gtgaagacgc gcgccaggcc ggtgacgtcg ctctgtccgt
      421 aggtgtaggt ggtggcgccg ccggtgagct gcggcgtgcc gtcgagattg agctcgacga
      481 ggccgatcgt gaacagctgc atcacctcgc gcgcatagtt ctcgtcgggc agcgagcccg
      541 aggccgggtt ggccttcatg ctgccgcgaa aggtgagcca catccccatc gccgggttgc
      601 tcgagatctg ttgcagcagc gcgcggtggt tgccgaacac gttggcttcg agcaggtcga
      661 ggtagccggc cgccgcgaac gctttccagc cgctgccgat cagtccgtcg atgccgacga
      721 cgaagatctc cgagaacgcg agcgtgatcc gctggcgcag cgtgtcgggc gaagacagga
      781 gcttttgcca ggtgacgctg tcgaagcccg cttcggtgtt cttgtacgac gggtcggcgt
      841 agccggcagc gacgagcgca tcccagcgcg tcggtgtcgc ggccatcgcg atctgctcgt
      901 cgagccacgc cgagtaaccg atcgcctgca cccgcgcgat ctgcgcgcgg ttggcgccca
      961 tcgaggcctg tgccaggaag cgcgacgctt cggcctcggt cggcggtggc gacgagcctg
     1021 agccgccggt gccggtcccg ccgccgtcgt tcgagccggc atcggaaccg ccgccgcacg
     1081 cggcgagcgt cgcggcggcg agcgatgccg cggacgtgcg accaagcgtc gtctgcaccg
     1141 gcgtcggcgg tggcgcaagc gcattcgccg gacctgccag gtcctcgatg tcttcgacgt
     1201 ccatcccatg accctccttg cgtgacagtg tccgacgaca acggccggca ccgatgaagg
     1261 gaagtcgaca cgtcggtaaa ggcgggtaaa ggaagtgcgt cgacttgcaa cgagctgcgc
     1321 agcgacgtga acaaagtcgc gatcgaccga ggaccaggga ctacggacgg cggaccgcgg
     1381 gccccgcgac cggcagaacg cgcaattcag ttcgacgaac ccgcgtagtt gcgcgggaca
     1441 agcacccgca cgacggtgcc tcggccggtc gccgtcgtca gctcgacctg cgcgccgagg
     1501 agctcgcaca gtcgcttgac gatcgccagc cccaggcctt cgccttgcgc ctggttcacg
     1561 ggccgcgcgt cggccgccgc gggcgcttcg cccggcacgg caccgagcgc gctgaccagc
     1621 ggcgcgccgg gcccggcatg gaatcccggc ccgctgtcgc gaacctcgaa ccaccagcga
     1681 ccatcggcca cgtcgccctc gccccaggtc agctcgacgc cgccttgcga ggtgtacttg
     1741 agggcgttga gaaccaggtt ctgggcgatc cgcctgacct tgacggcatc cccctcgaca
     1801 gcgagcgggc gcggcccccg cacatcgagg gagaggttcc tctcgccggc cagcggcctg
     1861 acgtcgtcgc cgaggcgttg cagcagctcg ccggcgtcga acgacgcgac gcgtcgtgtc
     1921 tcctgccccg cctgcaaccg ggccaactcg gtcacgtcgt cgagcaggcc gtgcaaggcc
     1981 ttgacgttgt ggcgcagcag gccgacgaaa tcgtcgcgcc gctggactgg cagatcgttg
     2041 atcgacaggc cgtgcgcgac gttggcgacg acgcccaggt tgccgcgcag gtcgtgcgcc
     2101 gcctgctgcc acagctcgcc acggcgccga tccgtttcct gcacatcggc gagcgccttc
     2161 tcgagatcgt gcagcgcacc cgcagcctcg agacgatcga ggcggaagta ctggcaggcg
     2221 ctttcgctcg tcgcctcgcc gatgaagaat gccagcgtcg cgtgagcctg cgcggcgagc
     2281 cggcgcgagg gcggcgaacg cgtggcgaga tacgactcga tctcgtcgac caggcagcgg
     2341 tgcagcgccg cccactcgcg cgtcacctcg tgcaggtcgt agccttgctg ccagcgctgc
     2401 aggccatgcg cctctgcgtt ccgtgtctcg cgatccgcgg cctgaaccgc ttcggcgccg
     2461 cccggcgccg cgccgagcgc acgggcgagc ccgtcgagcc acgccggcag atgatcgac
//
LOCUS       DASRWS010000304         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_703780, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000304 DASRWS010000000
VERSION     DASRWS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703780"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1497)
                     /gene="gltX"
                     /locus_tag="VFF72_05685"
     CDS             complement(85..1497)
                     /gene="gltX"
                     /locus_tag="VFF72_05685"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZV92688.1"
                     /translation="MHGRVRTRIAPSPTGFLHLGTARTALFSWAFARHHGGDFVLRIE
                     DTDVARSSQESVAQILASMRWLGLDHDEGPIYQMQRLARYREVAEELLATDKAYRCYA
                     SPAELEAMRESQRARGEKPLYDGRWRPEPGKVLPPVPEGVAPVIRLRNPRDGAVTWDD
                     LVKGPITISNHEIDDLVLVRADGVPTYNFAVVVDDHDMAISHVFRGDEHVNNTPWQIN
                     IFKALGAPLPVFAHVPVILGDDGQKLSKRRGAVSVTAYEDEGYLPDAMLNYLARLGWS
                     HGDEEIFTRQQIVDWFDGSHLARHPAQWDGAKLAWVNAHYLKGLPEGALVELAKAQLA
                     RRGIATPADDRALLAACLVFRERCNTGADLAGWLAMLYEDAPPSAADLAQYVVDSVRP
                     AVATLADRLEAAEWSVAGIAAAIKETLAAHGLKMAQLAPAVRVLVCGRPQTPSLDAVL
                     ALFSRPIVLHRLRTLPAASL"
     gene            complement(1521..2744)
                     /locus_tag="VFF72_05690"
     CDS             complement(1521..2744)
                     /locus_tag="VFF72_05690"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HZV92689.1"
                     /translation="MSVPKKIPRVDLPASARRDTRAVREGLPPSAWGENSEALFLTSS
                     FVQPDAASAVARFANEEEAFIYSRFTNPTVTMFERRLAALEGSESCIATSSGMSAILL
                     LGMGLLKAGDHVVCSQSVFGSTVMLFGREFAKFGIETSFVSQTDVAAWRDAVKPNTKL
                     LFAESPTNPLTEVCDIRALATVAHEAGALLAVDNCFCSPALQRPIEYGADIVVHSGTK
                     YLDGQGRVIAGALCASESLVEGKFVPVMRSAGMSLSPFNAWVVLKGLETLSIRMQAQG
                     ERALVLARWLEAQPGVARVFYPGLESHPQHALAMKQQSGGGAVVSFVVAGDDPETARR
                     NAFRVIDSTRILSITANLGDTKTTITHPASTSHGRLSEAQRQAAGIVQGMLRVAVGLD
                     DVLDIEDDLARGLAA"
     gene            complement(2744..4270)
                     /gene="purF"
                     /locus_tag="VFF72_05695"
     CDS             complement(2744..4270)
                     /gene="purF"
                     /locus_tag="VFF72_05695"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZV92690.1"
                     /translation="MCGIVGVISKSPVNQLIYDALLLLQHRGQDAAGIVTMQGTKCFM
                     QKARGMVRDVFRTRNMRALPGTVGLGQVRYPTAGNAYSEEEAQPFYVNAPYGIVLVHN
                     GNLTNAQSLKRELFDVDRRHINTGSDTEVLINVLAHELEGAAREWQLTADEIFAAVRA
                     VHGRVKGSYAVVALIAGQGLLAFRDPHGIRPLCFGEGEGPDGPEVMVASESVAIVGTG
                     HTLTRDVAPGEAIFIDLEGRVHSRQCADGATLNPCMFEFVYLARPDSVMDGISVYQAR
                     LNLGETLAQRLISTMPPSEIDVVIPIPESSRPSAMQLAQKIGKPYREGFVKNRYVGRT
                     FIMPGQGVRKKSVRQKLNAIGVEFKGRNVLLVDDSIVRGTTSKEIVQMARDAGAKKVY
                     LASAAPPVRFPNVYGIDMPTKDELIAHGRTIEEIRAFIGADALIYQDVAAMKRVVAAL
                     NPKLDGFDASCFDGRYVAGDVSADDVEAMAAQRARAPDEEDSGDDRSRLALRNAAEIV
                     "
     gene            complement(4290..>4790)
                     /locus_tag="VFF72_05700"
     CDS             complement(4290..>4790)
                     /locus_tag="VFF72_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZV92691.1"
                     /translation="WLPIGWVDIAMLGVVALSAVVGLWRGLTFELLSLAGWFAAYFGA
                     SWLVPWLAPHLPIGAPGSALNHGVAFASAFLAVLILWSLLSRAIAALVGATPLRPLDR
                     LLGAVFGVARAGVVLLAAATLVSFTPFSATAAWRQSSGAAMLGEALHRWLPRLTGDEA
                     VPARRV"
BASE COUNT          793 a         1619 c         1639 g          739 t
ORIGIN      
        1 gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat acccccatcg
       61 atgcggtttc aggaaagtcc acgattatag cgaggccgcc ggcaacgtcc gcagccggtg
      121 caacacgatc ggtctcgaaa agagcgcgag cacggcatcg agcgacggcg tctggggccg
      181 gccgcagacg agaacgcgca ccgcgggcgc cagctgcgcc atcttcaggc cgtgcgcggc
      241 gagcgtctcc ttgatcgccg cggcgattcc tgccacgctc cattcggcgg cctcgagccg
      301 gtcggccagc gtcgcgaccg ccgggcggac cgagtcgacg acgtattgcg cgaggtcggc
      361 agcgctcggc ggcgcatctt catagagcat ggcgagccaa ccggccagat cggcgcccgt
      421 gttgcagcgc tcgcgaaaga cgaggcaggc ggccagcaat gcgcggtcgt cggcaggagt
      481 ggcgatgccg cgtcgcgcca gttgcgcctt cgccagctcg acgagcgccc cctcgggcag
      541 gcccttcagg taatgcgcgt tgacccacgc cagcttggcg ccgtcccact gcgccggatg
      601 acgggcgaga tggctgccgt cgaaccagtc gacgatctgc tgccgggtga agatctcctc
      661 gtcgccgtgg ctccagccga gtcgcgcgag gtagttgagc atcgcatcgg gcaggtaccc
      721 ctcgtcttcg taggccgtca cgctgaccgc gccgcgccgc ttcgagagct tctgcccgtc
      781 gtcgccgagg atgaccggca cgtgcgcgaa gacgggcagc ggcgcgccca gggccttgaa
      841 gatgttgatc tgccagggcg tgttgttgac gtgctcgtca ccgcgaaaga cgtggctgat
      901 cgccatgtcg tggtcatcga cgacgaccgc gaagttgtag gtgggcacgc catcagctcg
      961 gacgaggacc agatcgtcga tctcgtggtt ggagatcgtg atcggcccct tgacgagatc
     1021 gtcccaggtg acggcgccat cgcgcggatt gcgaaggcgg atgacgggtg ccacgccctc
     1081 gggcaccggc ggcaggacct tgcccggctc gggccgccag cggccgtcgt agagcggctt
     1141 ttcgccgcgg gcgcgttgcg attcgcgcat cgcctcgagt tcggcgggcg acgcgtagca
     1201 gcgataggcc ttgtcggtcg cgagcagttc ctcggcgact tcccgatatc gcgcgagtcg
     1261 ctgcatctgg tagatcggac cctcgtcatg gtcgagcccg agccagcgca tcgaggccag
     1321 gatctgcgcc accgactcct ggctcgaccg ggcaacgtcg gtgtcttcga tccgcagcac
     1381 gaaatcgcca ccatggtggc gggcgaatgc ccaggaaaag agcgccgtgc gcgccgtgcc
     1441 cagatgcagg aagccggtcg gcgacggcgc gatgcgggtg cggacacggc cgtgcactgc
     1501 cggcgccgac gaggtgccgc tcacgctgcg agcccgcggg ccaggtcgtc ctcgatgtcg
     1561 agaacgtcgt cgaggccgac cgccaccctc agcatcccct ggacgatgcc ggcggcctgg
     1621 cgctgcgcct cgctgagccg accgtgcgag gtgctggccg gatgggtgat cgtcgtcttg
     1681 gtatcgccga gattggcggt gatcgaaagg atgcgggtcg agtcgatgac gcgaaaggcg
     1741 ttgcgccgtg ccgtctcggg gtcgtcgccg gcgacgacga agctgacgac ggcgccgcct
     1801 cccgactgct gcttcattgc cagcgcgtgc tgcggatgcg attcgagccc cggatagaag
     1861 acccgggcga cacccggctg cgcctcgagc cagcgagcca gcaccagcgc ccgctcgcct
     1921 tgcgcctgca tgcggatcga cagcgtttcc agccccttca ggacgaccca ggcattgaac
     1981 ggcgagagcg acatgccggc gcttcgcatc accggaacga acttgccctc gaccagcgac
     2041 tcgctcgcgc agagcgcgcc ggcgatgacg cggccctggc cgtcgaggta tttcgtaccc
     2101 gaatggacga cgatgtcggc gccgtattcg atcggccgct gcagcgccgg cgagcagaag
     2161 cagttatcga cggccagcag cgcaccggcc tcgtgcgcca cggtcgcgag agcacggatg
     2221 tcgcagacct cggtgagcgg attggtcggc gattcggcga acaacagctt cgtgttcggc
     2281 ttcactgcat cgcgccacgc cgccacgtcg gtctgcgaca cgaagctcgt ctcgatgcca
     2341 aacttggcga actcgcgccc gaacagcatc accgtcgagc cgaagacgct ctgcgagcag
     2401 acgacgtggt cgccggcctt gagcaggccc attcccagaa ggaggatcgc gctcatgccc
     2461 gacgacgtgg cgatgcacga ctcgctgcct tcgagcgcgg cgaggcgccg ctcgaacatc
     2521 gtcaccgtcg ggttggtgaa gcgcgagtag atgaacgcct cttcctcgtt ggcgaagcgg
     2581 gcgacggcgc tcgccgcgtc gggctggacg aagctgctgg tgagaaagag cgcctccgag
     2641 ttctcgcccc aggcgctcgg cggcaggccc tcgcgcactg cgcgcgtatc gcgccgcgcg
     2701 ctggcaggca ggtcgacgcg aggaatcttc ttcggcacgc tcatcagacg atctcggcgg
     2761 cgttgcgcaa ggcgagccgc gatcggtcgt cgccgctgtc ttcctcgtca ggcgcgcggg
     2821 cacgctgcgc cgccatcgcc tcgacgtcgt cggcgctgac gtcgcccgcg acgtagcgcc
     2881 cgtcgaagca cgacgcatcg aagccgtcga gcttcgggtt gagcgccgcg acgacgcgct
     2941 tcatcgccgc gacgtcctgg tagatgaggg catcggcgcc aatgaaggca cggatctcct
     3001 cgatcgtccg gccgtgcgcg atcagctcgt ccttggtggg catgtcgatg ccgtagacgt
     3061 tcggaaagcg caccggcggc gccgccgacg cgagatagac cttcttcgca ccggcgtcgc
     3121 gtgccatctg cacgatctcc ttgctggtgg tgccgcgaac gatcgagtcg tcgacgagca
     3181 ggacgttgcg gcccttgaat tcgacgccga tggcattgag cttttgccga accgacttct
     3241 tgcgaacgcc ctggcccggc atgatgaagg tgcggccgac gtagcggttc ttgacgaatc
     3301 cttcgcggta cggcttgccg atcttttgcg ccagctgcat cgccgacggc cggctcgact
     3361 cgggaatcgg aatgacgacg tcgatctccg aaggcggcat cgtcgagatc agccgctgcg
     3421 ccagcgtttc gccgagattg agccgcgcct ggtacaccga gatgccgtcc atcaccgagt
     3481 cgggccgggc gagatagacg aattcgaaca tgcaggggtt cagcgtcgcg ccgtcggcgc
     3541 actggcgtga atgaacgcgt ccttcgaggt cgatgaagat cgcctcgccg ggggcgacgt
     3601 ctcgagtcag ggtgtggccg gtgccgacga tggcgacgga ctcgctggcg accatcactt
     3661 cgggtccgtc gggaccttcg ccctcgccga agcacagcgg ccggatgcca tgcggatcgc
     3721 gaaacgccag cagcccctgg ccggcgatca gggcgacgac cgcgtacgag cccttgacgc
     3781 ggccgtgcac ggcccgcacc gcggcgaaga tctcgtcggc ggtgagctgc cattcgcgcg
     3841 cggcgccctc gagctcgtgc gccagcacgt tgatcaggac ctcggtatcg ctgccggtgt
     3901 tgatgtgacg gcgatcgaca tcgaagagct cgcgcttgag cgactgcgca ttcgtgaggt
     3961 tgccgttgtg cacgagcacg atgccgtaag gcgcgttgac gtagaagggc tgcgcctctt
     4021 cctcgctgta ggcgttccct gcggtcggat agcggacctg gccgaggccg acggtgcccg
     4081 gcagcgcccg catgttgcgg gtgcgaaaga cgtcgcgaac catgccacgt gccttctgca
     4141 tgaagcactt cgttccctgc atcgtgacga tgccggcggc gtcctggccg cgatgctgca
     4201 gcaacagcag cgcgtcgtag atcaactggt tgaccggcga cttcgagatc actccgacga
     4261 ttccgcacat ggctcgtgtc ctcagtctct caaacccgcc gggccggcac ggcctcgtcg
     4321 cccgtcagcc gcggcaacca gcgatgcaac gcctcgccga gcatcgcggc gccgctcgat
     4381 tgccgccagg ccgccgtggc ggaaaacggc gtgaacgaaa ccagcgtcgc cgccgcgagg
     4441 agaacgacgc cggcccgggc cacgccgaag acggcgccga gcagccggtc gagcggccgc
     4501 agcggcgtcg cgccgaccag tgccgcgatc gcgcgcgaaa gcaggctcca gaggatcagc
     4561 accgcaagga acgcagatgc gaacgcaacg ccgtgattca aggccgaacc cggggcgccg
     4621 atcggcaggt gtggggccag ccacggcacc agccagctcg ctccgaaata ggcggcgaac
     4681 cagccggcca gcgagagcaa ctcgaacgtc aggccgcgcc agagaccgac caccgccgag
     4741 agggccacga cacccagcat cgcgatgtcg acccagccga tcggcagcca
//
LOCUS       DASRWS010000305         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2414577, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000305 DASRWS010000000
VERSION     DASRWS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414577"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFF72_05705"
     CDS             <1..653
                     /locus_tag="VFF72_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZV92692.1"
                     /translation="RFDLGGMNVEGRITSLRKVDWSSMRVNFFVLFPSSSMPDAPVSF
                     IAAYRAPATPGFDNMLSRDFPNITSIDVSASIAEIQRVLDQVIRAVEFLFAFTLAAGL
                     VVLFAAVSATRESRAREFAVMRALGASGKLLAQVQRAELLGVGALAGVLASLAAVAVS
                     WALARYVFEFSWNPSPLVPVAGGAVGALLALAAGWWSLREVLQRPVVQTLRQAADV"
     gene            674..1525
                     /locus_tag="VFF72_05710"
     CDS             674..1525
                     /locus_tag="VFF72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HZV92693.1"
                     /translation="MKRQHQVLVAVVIAVVAIAVIVWFGWLRHHEEGESAAVAPVETP
                     AASAPIETPAPPEPASAPASAVIQHPIGAVDSEEATPDAATMLADVFGKRVAESLFVP
                     DDFAHRLAATVDGLGRVTASSRLWPAQPTPGHFSVAETNDGATVVAADNAERYAAFIG
                     IFERVDPIRVAQAYKRFYPQLQKAYQDLGYPDGYFNDRLVDVIDSMLAAPEPVGPVEV
                     RLPNVEGASAPARPWLVYEFADPALASLPAGQKLLVRMGVANERRVKALLLIFRGLVA
                     PGPAAAR"
     gene            1607..2749
                     /locus_tag="VFF72_05715"
     CDS             1607..2749
                     /locus_tag="VFF72_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92694.1"
                     /translation="MPAQPRDRVFRILHLEDSEVDHELMLAHLIRGGLTAETRRVESE
                     ASFLEALEEAWDVVVSDYNLPGFSGLVALELLKRHARDVPFVLVSGEIGEDTAVEAMR
                     NGASDYLQKSHLARLVPALLHAVDAAETRRARVKADRELVASKQRLHEFAQHLQTSIE
                     LERAAIAREIHDDVGGSLTALKFDLAWIARHSSSPQVVSRVQSALETVTHAVEASQRI
                     MHNLRPAILEQGLAAALHWIALRFERRAGVVCTIRLPKKPLDLPPGVPLVAYRTAQEA
                     LTNISKHARASRVQIDLSMAGGVLSLEISDNGRGLDQEDLAKARSFGIRGLHERATTV
                     GGWIDLSSGPHGTTLILSVPLDVPDSNYIPEAEPDRSYDPSQWADV"
     gene            2892..>3211
                     /locus_tag="VFF72_05720"
     CDS             2892..>3211
                     /locus_tag="VFF72_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV92695.1"
                     /translation="MIKVFLCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRVLMR
                     EKKAEVLVLDINLPGRSGLDVLHALKDEGSEMRVLVVSMYPEDQYALRALRAGAAGYV
                     NKAG"
BASE COUNT          471 a         1150 c         1086 g          504 t
ORIGIN      
        1 tgcgcttcga cctcggcggc atgaacgtgg aaggacgcat caccagcctg cgcaaggtcg
       61 actggtcgtc gatgcgggtc aacttcttcg tgctgtttcc ctcgtccagc atgccggatg
      121 cgccggtgag tttcatcgcc gcttaccgcg caccggcgac gcccggcttc gacaacatgc
      181 tgtcgcgcga cttcccgaac atcacctcga tcgacgtctc cgcgtcgatc gccgagatcc
      241 agcgtgtcct cgaccaggtg atccgcgccg tcgagttcct gttcgccttc accctggcgg
      301 ccggcctcgt cgtgctcttc gccgcagtca gcgcaacgcg cgagtcgcgc gcccgcgaat
      361 tcgcggtgat gcgcgccctc ggcgcgtcgg gcaagctgct ggcgcaggtc cagcgggccg
      421 agctgctggg cgtcggcgcc cttgccggcg tgctcgcctc gctggcggcg gtggccgtca
      481 gctgggccct ggcgaggtac gtcttcgaat tcagctggaa cccttcgccc cttgtcccgg
      541 tcgcaggggg agcggtggga gcgctgctgg cgctcgccgc cggctggtgg agcctgcgcg
      601 aagtgctgca acgaccggtc gtgcagacct tgcgccaggc ggcggacgta taagccggat
      661 cgaggaggca ggaatgaagc gacaacatca ggtgctcgtc gccgtcgtca tcgcggtggt
      721 ggcgatcgcc gtgatcgtct ggttcggctg gctgcgtcac catgaggagg gcgaaagcgc
      781 ggcggtcgcg cccgtagaga cgccggcagc gtcggcgccg atcgagacgc ctgccccgcc
      841 tgagcctgca tcggcgccgg cgtcggcggt catccagcat ccgatcggcg ccgtcgattc
      901 ggaagaggcc acgcccgacg cggcgacgat gctggccgac gtgttcggca agcgcgtcgc
      961 cgaatcgctc ttcgttcccg acgacttcgc gcatcgcctg gcggcgacgg tcgatggcct
     1021 cggccgggta acggcctcgt cgcggctttg gccggcgcag cccacgccgg gccacttcag
     1081 cgtcgccgag acgaacgacg gcgcgacggt cgtcgccgcc gacaacgccg agcgctatgc
     1141 cgcattcatc ggcatcttcg agcgggtcga cccgattcgc gtcgcccagg cctacaagcg
     1201 cttctatccg cagctgcaga aggcctacca ggacctgggc taccccgacg gctacttcaa
     1261 cgaccgtctc gtcgacgtca tcgattcgat gctcgcggcg cccgagccgg tcgggccggt
     1321 cgaggtccgg ttgcccaacg tcgagggcgc gtcggcgccg gcgcggccgt ggctggtcta
     1381 cgaattcgcc gatcccgcgc tcgcttccct gcccgcaggg cagaagctgc tcgtgcgcat
     1441 gggagtggcg aacgagcggc gggtcaaggc ccttctgctc attttccggg gcctcgtcgc
     1501 ccccgggccc gcagcggcgc gatagcgcgt ctcgactgca acacgcccgc ttcgggcgag
     1561 acgcagtgcg cagacgctcc tctcgtctcg cggcatactg cgtcacatgc cggcacagcc
     1621 acgggaccgc gtctttcgca tcctgcatct cgaagattcc gaggtcgacc acgagctgat
     1681 gctcgcgcat ctgatccgcg gcggcctgac cgccgagacc cgccgcgtcg aatcggaagc
     1741 atcgtttctc gaagccctcg aggaggcctg ggatgtcgtc gtctccgact acaacctgcc
     1801 gggcttcagc ggcctcgtcg ccctcgagct gctgaagcgg catgcccgcg acgtgccgtt
     1861 cgtcctcgtc tcgggcgaga tcggcgagga cacggcagtc gaggcgatgc gcaacggcgc
     1921 cagcgactac ctccagaaaa gccacctcgc gcgactcgtg ccggcgctgc tgcacgcggt
     1981 cgacgcggcg gagacgagac gcgctcgcgt caaggccgac cgcgagctgg tcgcctcgaa
     2041 acagcgcctg cacgagttcg cgcagcatct gcagaccagc atcgagctcg aacgcgcggc
     2101 gatcgcccgc gagatccacg acgacgtcgg cggctcgttg acggcgctga agttcgatct
     2161 cgcctggatc gcccgccatt cgagctcgcc gcaggtcgtc tcgcgggtcc agtcggcgct
     2221 cgagacggtg acccacgccg tcgaggcgag ccagaggatc atgcacaacc ttcgcccggc
     2281 catcctcgag caggggctgg ccgcggcgct gcactggatc gcgctgcgct tcgaaaggcg
     2341 cgccggcgtc gtctgcacga tccggctgcc gaagaagccg ctcgacctgc cgcccggcgt
     2401 gccgctggtc gcctaccgca ccgcccagga ggcgctcacc aacatcagca agcacgcgcg
     2461 tgcgagccgg gtgcagatcg acctgtcgat ggccggcggc gtgctctcgc tcgagatcag
     2521 cgacaacggc cgcggcctcg accaggaaga cctcgccaag gcgcgctcct tcggcatccg
     2581 cggcctgcac gagcgcgcga cgaccgtcgg cggctggatc gacctgtcga gcggcccgca
     2641 cggcacgacg ctgatcctgt cggtgccgct cgacgtaccg gacagcaact acattcccga
     2701 ggccgagccc gaccggtctt acgaccccag ccagtgggcg gacgtgtgaa gccggccccc
     2761 acgctcacgc gcacgttcgc tgcgtcccca aggggcgcgg ccggcctcgg gacggcccgg
     2821 cggccggccg atgaggtccc ctcgatcgcg ggcacgttcg ctgaccctcg ccgtcttcgc
     2881 caagcttcgc catgatcaag gtctttctct gcgacgacca cgcgctgatc cggcgcggca
     2941 tccgcgacac gctttccgat gcgcccgaca tcgaggtcgt cggcgaggcc ggcgactatg
     3001 gcgagttgcg cgtgctgatg cgcgagaaga aggccgaggt gctggtgctc gacatcaatc
     3061 tgcccggccg cagcggcctc gacgtgctgc atgcgctgaa ggacgaaggc agcgagatgc
     3121 gcgtgctcgt cgtctcgatg tacccggaag accagtacgc gctgcgcgcg ctgcgtgccg
     3181 gcgccgccgg ctacgtcaac aaggcgggtg a
//
LOCUS       DASRWS010000306         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_430240, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000306 DASRWS010000000
VERSION     DASRWS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430240"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFF72_05725"
     CDS             complement(<1..340)
                     /locus_tag="VFF72_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZV92696.1"
                     /translation="MPTTPTSYRDDPAAVRALVQPDRVHRDLYIDEEIFALEQEHFFA
                     NTWNYVGHDSQVPHAGDYLRAEIAGQPLIVVRHVDDTVRVLMNRCAHKGSRLVSAPSG
                     NTGRHFRCPYH"
     gene            complement(411..2063)
                     /locus_tag="VFF72_05730"
                     /pseudo
     CDS             complement(411..2063)
                     /locus_tag="VFF72_05730"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
BASE COUNT          303 a          766 c          831 g          283 t
ORIGIN      
        1 cgtggtacgg gcagcggaag tggcggccgg tgttgcccga cggcgcgctg acgaggcgcg
       61 accctttgtg cgcgcagcgg ttcatcagca cgcgcaccgt gtcgtcgacg tggcgaacga
      121 cgatcagcgg ctggccggcg atctcggcgc gcaggtagtc gcccgcatgc ggcacctgcg
      181 agtcgtggcc gacgtagttc caggtgttgg cgaagaaatg ctcctgttcc agagcgaaga
      241 tctcctcgtc gatgtagagg tcgcggtgga cgcggtcggg ctgcaccagg gcgcgcaccg
      301 ctgccggatc gtcgcggtag gaggtcgggg tcgtcggcat ggctgtctcg ctgcgtgcgt
      361 cggtgcgtgc ggtcgctctg aaggttcctc tcaggccagc gcgtgccggc cagcgtcgcc
      421 cgcagtgtct caccctgctc gtcggcgagc gccgcctcgc gcagctcgcg cagggtcgaa
      481 ggcgccagcg acgacccggc gcggcgaatg gcagccatca gcatgtcgaa atggcgcacg
      541 ccgcgctcgt gggtgtcgct gtagcccttg accaggcgct ggcattgcgc cacctcgacg
      601 gcaagctccg gatcgcgacc ggccgtctcg ccgatgcagg cgagccaggc gacgatggcg
      661 gcgtcctcgg cggcgtagcg cgtcgtcgag cggcgccagc gcctcatgcc ggcgaccgtc
      721 cacagcatca ggaagccgcc gagcgaactg gtcgtgacga cgcggccctt cctcgtgaag
      781 cgctcgacga gccggcgcag cgcacccggc cgctcgatcc ggcggccgag ccagccgggc
      841 agcgtctcgg cgatctcctg aacccgcggg tgcaggtact cgtcgatcgc cagcacctgc
      901 ccggcgccgg agcgcacctc gctgcgcacg cgctcgaagc gcgagccgcg cgtcttcagc
      961 tgggcgacgc gcatcgtgtc ctcgtacgac atccacagcg cgaggtggcg cgcggtctcg
     1021 caaaggagcg caggcgccgc cgtcgacggc gcgacgagcg gggccaggcg gtcgagataa
     1081 aggccggcgt aggccgggtc ctggtagtcg acgaggcggc gcaggccctc gcgcacgacg
     1141 ccgtgcgagg cgggcggaat ctcgctctcg agccggccga gcagggcccg gacgcgcgcg
     1201 tcgacgggac gcggggtggc cgtcgacgca tcggaggcgg gagacgccga agaagcgacc
     1261 cgcaacgcgc cggcgtcgga cgcgtcgcgg ccgccgacgg agccgcggac ggacccgtcg
     1321 gcggcaccgt cgggcgcgtc ggtgccgccg tcgagggcgg cggccgccgg ctctgcgacg
     1381 gtctggacgc ggtcgtcggt cggcgctgct ccggcatcgc cgcggacacg cacatcgccg
     1441 ccggcttcgt cgaaggcgag cgcgaacgcc ctcaggctcg gcgcgacacc gacgccgccg
     1501 cgcgtgatcg tcgcctcgaa ctgctcgcgc gagaacggca gcacaccggt gccggcgagc
     1561 gcgccgaaca gcacggcgct cagcacgctg ccggtacgct cggcagcttc ggccatgtcg
     1621 aagtggacga agcgcttcgc cgctgccgcg gcgtgcgcga gaagggcgtc gccatcgacc
     1681 cggccgtcgc cgagcgccgt cttctcggcg atcgagtaga cgcgatgcgt cgaggcgacg
     1741 agcgtggtgc ggtcgggcgt gacgaggccg cgctgcacgg cgcggccgca ttccatgagc
     1801 tcggaggcca gcaccacgtc gacgtcgccg ggcagcggca tcaatgcaag gaccgggacg
     1861 ccgccgtcga tctcggcctg gcggcgcgga tagagctcga cgtagtagat ggtggcgccg
     1921 gtgcgctgcg cgacgccggg caccgaggtc gtctgcgcga agtggtcgtt cgattcgccg
     1981 aggtcgacga tccagtcggc gagcacgccg ccgccctcgc cgcccatggc gaggatggcg
     2041 atcttgatcg gctgcgcgct caagccggct ccgcgctcag aacgcgagcc gggcgcgcgc
     2101 cctggcgtcg cgccgctgca gccagccgat caccgcgccg cggatgcgga ccatgaggcg
     2161 atcccgcctc gacgggttgg tga
//
LOCUS       DASRWS010000307         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2144518, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000307 DASRWS010000000
VERSION     DASRWS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144518"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFF72_05735"
     CDS             complement(<1..578)
                     /locus_tag="VFF72_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV92697.1"
                     /translation="MLHIVDGKVVAKKKRTILARFAAFLCDPFVSTWRHRDLVDAILR
                     RELAERFKGSVAGWVWAITAPLLSLIVYTVAFAGGPVSLPGGGKSGSNFDYALFIFGG
                     LIAFNLFTEMAYRAPSLLHEYAHFIKQTIFPAEMLPIISTLRATFYATIGLALMLVAQ
                     FALTGTLHLTVLLLPLWFVPFVAFLIGLTWILS"
     gene            complement(680..1744)
                     /locus_tag="VFF72_05740"
     CDS             complement(680..1744)
                     /locus_tag="VFF72_05740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZV92698.1"
                     /translation="MEFGWLTLALSPSPEEDAERIGQLIEQACLAERLGFSDVWLTEH
                     YFTGESVYNDSLLFASALAMRTERVRIGFAVVQTPFHHPVRLATQLALLDNLSNGRID
                     VGLGKGTVYNEYEFVGHGLTSADSRERMAETVEILERAWSGEPFRYDGQFHRVQVPAL
                     RPKPVQQPGPPLWRSVISPASFEECGRLGLPILTARLPVARIRERWALYDKGLQAGGH
                     DEATRDRLLGRSALWRNVYVADSQAQAEDELANLLVETRAHMMHVREAYNPPDFSPAP
                     ETLNPWTDPRVADDVAIPYVLETGSLYGTPKRVTEQVAELQEAGVRHLLCQTGFGAMP
                     HDLNVASMRRFGEQVMPRFR"
     gene            1968..2735
                     /locus_tag="VFF72_05745"
     CDS             1968..2735
                     /locus_tag="VFF72_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZV92699.1"
                     /translation="MLKIVKDCVLKVSRSFGYDIVPLREMKERDFALHLRALLNFLKV
                     DCVLDVGANVGQYHDFLRDKVLYDGPIVSFEPVSRHVDFLRERARTDGGWHIEGYALG
                     AKDGNLPINVMVSDQFSSFLQPDNSRVRAYDGLNVPCHVENVLVRTLDVVMPVLQQKL
                     GFQRPYLKLDTQGFDIEVLQGARDSLAEVRALQTEASVIGIYKGMPGYMDTIRYLDER
                     GFDITGFYPVSRDSSLRLVEFDCVMINRAAAAVQAAA"
     gene            complement(2698..>3209)
                     /locus_tag="VFF72_05750"
     CDS             complement(2698..>3209)
                     /locus_tag="VFF72_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92700.1"
                     /translation="AERLRGRVHFKFRGALTTVDPASFHTALARLPNVEFGGPYVPHK
                     DLEELYSSVDFAWALDLEHTDHNSRWLMPCRFYESGYFGVPCLAVRSFEVGSVLERHH
                     IGFTFERPLEEQLVHFFETLTVADYEHIRARLRAVPREMFVADDDATRLCSMMEGLKQ
                     QPEQQPQRG"
BASE COUNT          609 a          993 c         1059 g          548 t
ORIGIN      
        1 gacagtatcc aggtgaggcc gatcaggaag gccacgaacg gcacgaacca tagcggcagc
       61 aacagcaccg tgagatgcag cgtgccggtc agcgcaaact gggcgaccag catcagggcg
      121 aggccaatcg tggcgtagaa cgtcgcccgc agcgtcgaga tgatcggcag catctccgcc
      181 ggaaagatgg tctgcttgat gaagtgcgca tactcatgca gcagcgacgg tgcgcgatag
      241 gccatctcgg tgaagaggtt gaaggcgatc agcccgccga agatgaacag cgcgtagtcg
      301 aagttcgagc cggacttgcc gccgccgggc agcgacaccg ggccgccggc gaaggccact
      361 gtgtagacga tcagcgacag cagcggcgcg gtgatggccc acacccagcc cgcgaccgat
      421 cccttgaatc gctcggcaag ctcacgtcgc aggatggcgt cgaccagatc gcggtggcgc
      481 cacgtggaga cgaaaggatc gcaaaggaac gcagcaaagc gcgcaagaat cgtgcgcttt
      541 ttcttggcga caaccttgcc atcaacgatg tgcaacaacg gcaattctac tcacctggcc
      601 ggaagggggt cggcctctct tcggccgcct tatctatagt accacgccgg cggtgcaact
      661 gcttcgcccg gtgggttttt caccggaatc tcggcattac ctgctcgccg aaccggcgca
      721 tcgacgcgac gttgagatcg tgcggcatgg cgccaaaacc ggtttggcag aggagatggc
      781 gcacacccgc ctcctggagc tccgcgacct gctcggtcac ccgcttgggc gtcccgtaga
      841 gcgagcccgt ttcgaggacg taggggatgg ccacgtcgtc ggccaccctg gggtcggtcc
      901 agggattgag cgtttcgggg gccggcgaga agtcgggcgg attataggcc tcgcgcacat
      961 gcatcatatg ggcgcgcgtc tcgaccagga ggttggcaag ctcgtcctcc gcctgcgcct
     1021 gggagtcggc gacgtagaca ttgcgccaga gggcgctgcg gcccagcagc cgatcgcgcg
     1081 tggcttcgtc atggccgccg gcttgcaggc ccttgtcgta aagggcccag cgctcgcgga
     1141 tccgcgccac cggcagtcgc gcggtcagga tcggcaggcc gagccggccg cattcctcga
     1201 acgaggctgg cgagatgacg ctgcgccaga gcggcgggcc cggctgctgc accggcttgg
     1261 gccgtagcgc cggcacctgg acgcgatgga actggccatc gtagcggaag ggctcgccgc
     1321 tccaggcgcg ctccaggatc tcgacggtct ccgccatccg ctcgcggctg tcagcgctcg
     1381 tgaggccgtg acccacgaac tcgtactcgt tgtagaccgt gcccttgccg aggccgacgt
     1441 cgatgcggcc gttgctgaga ttgtcgagca gtgccagttg ggtggcgagc cggacggggt
     1501 gatggaacgg cgtttgcacg acggcaaagc cgatgcgcac gcgctccgtt cgcatggcaa
     1561 gtgcggaagc gaacagtagc gagtcgttat agacactctc gcctgtgaaa tagtgctcgg
     1621 tcagccacac gtcgctgaaa cccaaccgct cggccaggca ggcctgctcg atcagctggc
     1681 cgatgcgttc ggcatcctcc tcgggcgacg gcgatagcgc caaggtcagc cagccaaatt
     1741 ccatcctcaa gtccctcacg cgaacgcgat ggagtaaaaa ctagcaccaa acagcggttc
     1801 ctcgtacgaa taggtctccc agacccaagc ttgtggacag acgctgccgt agcgtttcgt
     1861 ccctggcacg gtgcgggcaa tatgccagcg gcgtgacaac gcatctgtga ccggcagcca
     1921 ccgaatgatc actttaaagg cacggggcac tgacgaatgc ttgcgacatg ctaaagatcg
     1981 ttaaggattg cgtgctgaaa gtcagccgct cgttcggcta tgacattgtc cctctgcgcg
     2041 agatgaagga acgcgacttt gcgctccatc tgcgcgcgtt gcttaacttt ttgaaggtcg
     2101 attgcgtgct cgatgtcggc gccaatgtcg gccagtacca tgacttcttg cgtgacaagg
     2161 ttttatacga tggcccgatc gtgtcgttcg agccggtcag ccgtcatgtc gacttcctgc
     2221 gcgagcgcgc ccgcaccgac ggcggctggc acatcgaggg ctatgcgctg ggcgccaagg
     2281 acggcaacct gccgatcaac gtcatggtat ccgaccagtt cagctcgttc ctgcagccgg
     2341 acaacagccg cgtacgcgcc tatgacgggc tgaacgtgcc ctgccatgtc gagaacgtgc
     2401 tggtgcgcac actcgatgtc gtgatgccgg tgctgcagca gaagctcggc ttccagcggc
     2461 cctatctcaa gctcgacacg caaggcttcg acatcgaggt actgcagggt gcgcgcgaca
     2521 gccttgccga ggtacgggcg ctgcagaccg aggcgtcggt catcggcatc tacaagggca
     2581 tgccgggata tatggacacg atccgctatc tcgacgaacg cggcttcgac atcaccggct
     2641 tctatccggt gagccgcgac agctcgctgc ggctggtcga gttcgactgc gtgatgatca
     2701 accgcgctgc ggctgctgtt caggctgctg cttaagccct tccatcatcg agcagagccg
     2761 cgtcgcgtcg tcgtcggcga cgaacatctc gcgcggcacg gcgcgaaggc gagcgcggat
     2821 gtgctcgtag tcggcgaccg tcagggtctc gaagaaatgc accagttgct cctcgagcgg
     2881 ccgctcgaag gtgaaaccga tgtggtggcg ctccagcacc gagccgacct cgaagctgcg
     2941 cacggcgagg cacggcacgc cgaagtagcc cgactcgtag aagcggcagg gcatcagcca
     3001 gcgtgaattg tggtcggtgt gctcgaggtc gagcgcccag gcgaagtcga cgctgctgta
     3061 gagttcctcg agatccttgt gcggcacgta gggaccgccg aactccacgt tgggcagccg
     3121 cgcaagggcg gtgtggaacg aggccggatc gaccgtggta agggcgccac ggaacttgaa
     3181 gtggacgcgg ccccgcaggc gttcggcca
//
LOCUS       DASRWS010000308         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1279484, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000308 DASRWS010000000
VERSION     DASRWS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279484"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFF72_05755"
     CDS             complement(<1..155)
                     /locus_tag="VFF72_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HZV92701.1"
                     /translation="MSKAFTREGAGDEADDDDTDDAHGAAAPLPAGTKNYLTPEGYSR
                     LRAELMQL"
     gene            277..843
                     /locus_tag="VFF72_05760"
     CDS             277..843
                     /locus_tag="VFF72_05760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZV92702.1"
                     /translation="MASPHHVAVVVGSLRKGSFSRKIALALKAMAQPPLALEIVEIGD
                     LPLFNQDFESDLPPAVVAFKAAVAKADAVLFVTPEYNRSVPGVLKNAIDCASRPYGKS
                     AWSGKPAAVLSNSPGAMGGFGANHHLRQSLVFLDMPTLAQPEAYVGQAGELLDEHGQW
                     KKPETKQFMEKFLAAFAAWIEKNAKKAG"
     gene            complement(871..2310)
                     /locus_tag="VFF72_05765"
     CDS             complement(871..2310)
                     /locus_tag="VFF72_05765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HZV92703.1"
                     /translation="MIRRLARLSITGRLVAMFAAATLATFAVIGAVLYVVMQHELVRH
                     EDEELNTNVQNLTYSIERAGTTDRWGRVQAKMDTLTPPDGSIRFWVLSDDPRFQYGKG
                     LAAIETMLRNDDGSGTIALPSAAAPLRTMTRDISAFQDRPAVRLIVASDTGPYLRTMR
                     VFLVALVGLSLATMLAVVLVGTWIARVGLRPLERLSREARALRPKTPAQRLQVSHLPV
                     ELADFAVAFNGAIGRLEDAYRQLEAFNADVAHELRTPLANLIGGTQVALARPRSAAEL
                     NEILQSNLEELEQLRLIINDMLFLARADQGEVATGLVRTVLADEVRKTIDFYEVLLDD
                     LGAAVSIEGDVDTEASIERALFRRALSNLLQNAIEHSAPGARIVVEIRRGESDVWVSV
                     VNPGEPIAESHVRRLFDRFYRVDAARNVPGRPHGHGLGLAIVKAVATMHSGSVAAASA
                     GGRTTIGFSVPLASPFVVAPARVRSDVPG"
     gene            complement(2307..>2636)
                     /locus_tag="VFF72_05770"
     CDS             complement(2307..>2636)
                     /locus_tag="VFF72_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZV92704.1"
                     /translation="QEPMQIKIANLQIDLLARKAWRGATRIDLTGKEFALLAVLARRQ
                     GEILSKTAIAELVWDMNFDSNTNVVEVAIKRLRAKVDAGFAPRLLHTIRGMGYVMEVR
                     DDAGAEP"
BASE COUNT          408 a          915 c          898 g          415 t
ORIGIN      
        1 agctgcatca gctcggcgcg caggcgcgag tagccctcgg gagtgaggta gttcttcgta
       61 ccggcaggca gcggcgcggc ggcgccgtgg gcgtcgtcgg tgtcgtcgtc gtcggcttcg
      121 tcgccggcgc cttctcgggt gaaagccttg ctcacggtcg tccttgatcg ccacggccga
      181 cgcgtcacgc gccgccaagg catcatcatc gccgcaatcg cctcggccgc ccggccgtgc
      241 gcgttcctgt cccctcgaac cgaaaggatc caattcatgg cctcccccca ccacgtcgct
      301 gtcgtcgtcg gcagcctgcg caagggctcg ttcagccgca agatcgcgct cgcgctcaag
      361 gccatggcgc agccaccgct ggcgctcgaa atcgtcgaga tcggcgactt gccccttttc
      421 aaccaggact tcgagtccga tctgccgccc gccgtggtcg ccttcaaggc agccgtcgcc
      481 aaggccgacg cggtgctctt cgtgaccccc gaatacaacc gatcggtgcc cggcgtgctg
      541 aagaatgcca tcgactgcgc ctcgaggccc tacgggaaaa gcgcgtggag cggcaagccc
      601 gcggccgtgc tcagcaactc gccgggggcg atgggcggat tcggcgccaa ccaccacctg
      661 cgccagtcgc tcgtcttcct cgacatgccg acgctggcgc agcccgaggc ctacgtcggc
      721 caggccggcg agctgctcga cgagcacggg cagtggaaaa agcccgagac gaagcagttc
      781 atggagaagt tcctcgccgc cttcgccgcc tggatcgaga agaacgcaaa gaaggcgggc
      841 tgacgcggcc cggcgccggt cggtacgacg tcagcccggg acgtcgctgc gcacgcgcgc
      901 cggcgcgacg acgaaaggcg acgccagcgg caccgaaaat ccgatcgtcg ttcggccgcc
      961 cgcgctggcc gcggcgacgc ttccggaatg catggtcgcc accgccttga cgatggccag
     1021 gccgagcccg tgaccatgcg gacgtccggg cacgttgcgc gcggcatcga cgcggtagaa
     1081 gcggtcgaac aggcggcgca cgtggctttc ggcgatcggc tcgccggggt tcacgaccga
     1141 gacccacacg tcgctctcgc cgcgccggat ctcgacgacg atccgtgccc ccggcgccga
     1201 gtgctcgatc gcgttttgca agagattcga cagggcgcgg cgaaagagcg cgcgctcgat
     1261 cgaagcttcg gtgtcgacgt cgccttcgat cgacacggcg gcgccgaggt cgtcgagcag
     1321 gacttcgtag aagtcgatcg tctttcgcac ctcgtcggcc agcacggtgc gcaccaggcc
     1381 ggtggccacc tcgccctggt cggcacgcgc caagaacagc atgtcgttga tgatgagccg
     1441 cagctgctcg agctcttcga ggttcgactg caggatttcg ttgagctcgg cggcgctgcg
     1501 cggccgcgcc agggccacct gggtgccgcc gatcaggttg gcgagcggcg tcctcagctc
     1561 gtgggcgacg tcggcgttga aagcctcgag ctggcggtac gcgtcctcga gacggccgat
     1621 cgcgccgttg aaggcgaccg cgaaatcggc cagctcgacc ggcaggtgcg agacctgcag
     1681 ccgctgtgcc ggcgtcttcg gccgcagtgc cctggcttcg cgcgaaagac gctcgagcgg
     1741 ccgcaggccg acgcgggcga tccaggtacc gacgagaacg accgcaagca tcgtcgccag
     1801 cgacaggccg acgagggcta cgaggaagac gcgcatcgtc cgcaggtagg ggccggtatc
     1861 gctggcgacg atcaggcgaa ccgcgggccg atcctggaac gccgagatgt cgcgcgtcat
     1921 cgtgcgcagc ggcgctgccg cgctcggcag agcgatggtg ccgctgccgt cgtcgtttct
     1981 cagcatcgtc tcgatcgccg ccagtccctt gccgtactgg aaccgcggat cgtcgctcag
     2041 cacccagaag cggatgctgc cgtcgggcgg cgtcagcgtg tccatcttcg cctgcacgcg
     2101 tccccagcgg tcggtcgttc cggcgcgctc gatcgaatag gtgaggttct gcacgttggt
     2161 gttgagctcc tcgtcctcgt ggcgcacgag ctcgtgctgc atgacgacgt agagcacggc
     2221 gccgatgacc gcgaaggtgg ccagcgtcgc cgccgcgaac atcgcgacca gccggccggt
     2281 gatcgacagc cgagcgagcc gtcggatcac ggctcggccc ccgcgtcgtc gcgcacttcc
     2341 atgacgtagc ccatgccgcg gatggtgtgc agcaggcgcg gcgcgaaacc ggcgtcgacc
     2401 ttggcccgca gccgcttgat cgcgacctcg acgacgttgg tgttgctgtc gaagttcatg
     2461 tcccagacca gctcggcgat cgccgtcttg gagagaatct cgccctggcg gcgcgccagc
     2521 acggcgagca gcgcgaactc cttgcccgtc aggtcgatcc gggtcgcgcc gcgccaggcc
     2581 tttctcgcca gcaggtcgat ctgcaggttg gcgatcttga tctgcatcgg ctcctg
//
LOCUS       DASRWS010000309         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1279539, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000309 DASRWS010000000
VERSION     DASRWS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279539"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VFF72_05775"
     CDS             <1..1231
                     /locus_tag="VFF72_05775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV92705.1"
                     /translation="GVGKGHAALRTALAGNVEQTYILIEAPLDRRYADLIRTYGGDVR
                     FAEDLEDVARLAREHDVVQFEFWNHPRLFECLARTDFAAIRSVFWAHVSGLFTPVVPP
                     GLVAEAGRFVFSTEASLTIPWLAEIPHDRIAVIRSGFGFPPRLPERAVDGLPETAYLG
                     TVDFVKMNPGFFEVVDALDMDALVRVWGECSTPVIARALAMNNPQRIRFCGQTATPAA
                     ALANAEIFFYPLQPAHYGTAENALVEAMSLGLVPVVLNNPAETAIVQDGQTGLVGETI
                     DHCVALLQSLLTSVSLRDRLSRNAAAAMAATRTPLRSAAAFVALWQSLLAEAPRRHDF
                     ASAIGDMPGDWYAATQRLPGAAWTPIIEPDAPTSKGTFAHFEHVFPDDRSLALLRAES
                     VVRQAGGMPHFTRAVAI"
     gene            1243..2262
                     /locus_tag="VFF72_05780"
     CDS             1243..2262
                     /locus_tag="VFF72_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HZV92706.1"
                     /translation="MGIEMDRRKIFMLPKAYPREQLGDLLPLPNPISMQVDPASLCNF
                     KCAFCPTGDPKLIKQSGRAQVFMKFELFEKIVADIAKFERPLKVLKLFKDGEPMLNPR
                     FVDMVALAKREPKIERVETTSNGSKLAPAFNERLVASGIDRVVLSIEGVTAERYLSFA
                     RVRFDYDAFVENVRHLFSIRGGMKLHVKTVAQNLDYTLGEDRKFFDTFGPISDGIYIE
                     NTVSSWPQFSVAGAVHEDVDAYGRETVHKELCPYPYYSISINADGVVSPCCVDWNRDL
                     AIGSVATESLTDIWNGQALRALRRQHLDEGLGANPSCASCGQVHACTHDNLDGHKAKL
                     KSLFA"
BASE COUNT          404 a          789 c          730 g          432 t
ORIGIN      
        1 cggcgtcggc aagggccacg cggccttgag gactgcgctc gccggtaacg tcgagcagac
       61 ctatatcctt atcgaggcgc cgctcgatcg ccgctatgcg gacttgatca ggacctacgg
      121 cggggacgtg cgtttcgccg aagatctgga agatgtcgcg cggctggcgc gcgaacacga
      181 cgtcgtccag ttcgaattct ggaaccatcc ccgtctcttc gagtgccttg ctcgcaccga
      241 ttttgcggcc atacgcagcg ttttctgggc gcacgtttcc ggcctcttca cgccggtcgt
      301 cccccccggc ctggttgccg aggcgggccg tttcgttttc agcaccgaag catcgctgac
      361 gatcccgtgg ctggccgaga taccgcacga ccgcatcgcg gtgatccgca gcggtttcgg
      421 cttcccgccg aggcttccgg agcgtgcggt ggacggcctt cccgagacgg cgtatctcgg
      481 caccgtcgat ttcgtcaaaa tgaatccggg cttctttgaa gtcgtcgatg cgctcgacat
      541 ggatgcactg gtgagggtgt ggggcgaatg ctccacgcca gtgatcgcgc gtgcgctggc
      601 gatgaataac ccgcagcgca tccgcttctg cggtcagacc gccacgcccg cggccgcgct
      661 cgccaatgcc gagatctttt tctatccgtt gcagccagcc cactacggca cggccgaaaa
      721 cgcgctcgtc gaagcgatgt cgctcgggct ggtgccggtc gtgctgaaca atcccgcgga
      781 aaccgcgatc gtccaggacg ggcaaaccgg ccttgtcggc gaaaccatcg accattgcgt
      841 cgcgcttctg caatcgctct tgacttctgt gagcctgcgc gaccgcctgt cgcgcaatgc
      901 ggcggcggcg atggcggcga cgcggacgcc gttacgcagc gcggccgcct tcgtcgcgct
      961 ttggcagtcg ctgctcgcgg aggcgccacg ccggcacgac ttcgcaagcg ccatcggcga
     1021 tatgcccggc gattggtacg cggcgacaca gcgcctgccc ggcgcggcat ggacgccgat
     1081 catcgagccg gacgccccga cgtcgaaagg cactttcgcg catttcgagc acgtgtttcc
     1141 cgacgatcgt tcgctggcgc tgctgcgcgc cgagagcgtc gtgagacagg ccggcgggat
     1201 gccgcatttc acaagagcgg ttgcgatcta agggagcgcc cgatgggcat cgagatggac
     1261 cggcgaaaga tcttcatgtt gcccaaggcc tatccgcgcg agcagctcgg ggaccttctg
     1321 ccactgccga acccgatctc gatgcaggtc gacccggcat cgttgtgcaa cttcaaatgc
     1381 gccttttgcc cgactggcga tccgaagctc atcaagcaat ccggccgcgc ccaggtcttc
     1441 atgaagttcg agctgttcga aaagatcgtc gccgacattg cgaagttcga gcgcccgctc
     1501 aaggtgctca agctgttcaa agacggcgag ccgatgctca atccgcgctt tgtcgacatg
     1561 gtcgcgcttg ccaagcggga gccgaagatc gaacgcgtcg aaacgacgag caacggctcg
     1621 aagctggcgc cggcattcaa cgaacggctg gtcgcctccg gcatcgaccg agtcgtgctg
     1681 tcgatcgaag gcgtcaccgc cgaacgctat ctctccttcg cccgcgtccg cttcgattat
     1741 gacgccttcg tcgagaacgt acgccacctg ttctcgatcc gaggcggcat gaagcttcac
     1801 gtcaagacgg tggcgcaaaa cctcgattat acgctcggcg aggaccgcaa gttcttcgac
     1861 acgttcgggc cgatctccga cgggatctac atcgagaaca ccgtttcgtc gtggccacag
     1921 ttctcggtcg ccggcgcggt gcacgaagac gtcgacgcct atggccgcga gaccgtgcac
     1981 aaggagctct gcccttaccc gtattactcg atctcgatca acgccgacgg cgtcgtcagc
     2041 ccgtgctgcg tcgactggaa tcgtgatctc gcgatcggct cggtcgcgac cgagagcctc
     2101 accgacatct ggaacggcca ggcgctccgc gcactgcgcc gccagcatct cgacgagggc
     2161 ctcggcgcca atccctcctg tgcctcctgc ggtcaggtgc acgcctgcac gcacgacaat
     2221 ctcgacgggc acaaggcgaa gctgaagtcg ctgttcgcgt gacgagcccg gccggcacat
     2281 acaaggacgc cctggcgcgc ggacgctacg cgctcatcgg cgccggccaa ctcggcgcca
     2341 tgtcgctgcg gcttt
//
LOCUS       DASRWS010000310         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2145182, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000310 DASRWS010000000
VERSION     DASRWS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145182"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFF72_05785"
     CDS             complement(<1..937)
                     /locus_tag="VFF72_05785"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HZV92707.1"
                     /translation="MNPNERRRAFVSLPFAAAGAAWGKGALGQVAASAAAAGDDSPTR
                     LALLIGNRSYPAPFDLPPVYKNVRDLGGALQARGFKVTSALDQNPGQMQAAIQAFSKV
                     AAASPPDATILFYYTGHGMQIDAENLMLGSGVAPDARVETLVSSSLQLRRDVIDQLPR
                     RASGLTIAVVDACRRSLRAALKATDGFNQVEAPLGCLIVFSTGAGKPAIAPAVDTLNT
                     FYTASLVKLLGSSDGETSFSDLFRLVKFDVQETMENYPVAAIRQFTQIPFIAENTQVR
                     FPLALRPEAQAAPPKFSAEDEAAAWRSLEESLWPAD"
     gene            complement(934..>2621)
                     /locus_tag="VFF72_05790"
     CDS             complement(934..>2621)
                     /locus_tag="VFF72_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92708.1"
                     /translation="ARSSAAPSRGRTSCSGSGTLSIAPVDATLSVGVAGGAGALKVSQ
                     AVLDGAIGFGTHVIGRADGSGAISAGNLTLRADTTLQTGSGDITVSGSIDGPFALALD
                     SGGTTRISGPIGVTAPLKSLTTDNNPAAADWDGTSGERTLFDTADGSGSARVFTTGSQ
                     TYGDPVTASVPILFSGGAISAMQATNQFAAPVAANADSLQLHSSLDLNLGSITLANGG
                     AVDTDGVLHLTGSVQLAAGTLSFTANATPTAIDITDADFQGKPFNFGFVPIKEASATI
                     VEDVGASLSSASGSLIAFRSPNGGTLQLDQPGNTLLGGISAVSGKLDDNDLSRFNSAS
                     TLTLGFIRIASSEIHVAGSPPADGDQTTLPAGIEGDVVKLTANVLATGTDGLIRARLP
                     YNNVQGSQTSIPGITFVLTPTALATGNGAFGSPASDNYIRVQVGGAEGGFVTADPKGV
                     GGENAVIFLGGNADVRPFYDENGKLSEIRIFYNGDAPRTPQEAGALAAVIALIEEARH
                     ERFEEAVRTENVSSRLRSGVIAEVGAGRPATVGRESIRLPDTCDLKPGTLRCE"
BASE COUNT          392 a          840 c          942 g          447 t
ORIGIN      
        1 cgtcggccgg ccagaggctt tcctcgaggg accgccatgc ggcggcttcg tcttcggcgc
       61 tgaacttcgg cggcgcggcc tgggcctcgg ggcgcaatgc cagtggaaaa cgcacctggg
      121 tgttctcggc gatgaagggg atctgcgtga actggcggat cgcggcgacc ggatagttct
      181 ccatcgtctc ctgcacgtcg aacttgacga ggcgaaacag gtcggaaaac gacgtctcgc
      241 cgtccgacga gcccagcaac ttgacaagcg atgcggtata gaacgtattg agcgtatcga
      301 cggccggggc gatcgcgggt ttgcccgcgc cagtcgaaaa gacgatcagg cacccgagcg
      361 gcgcttccac ctggttgaac ccgtcggtgg ccttgagggc tgcgcgaagc gacctgcggc
      421 aggcatccac gacggcgatc gtcagtccgg acgcgcgccg cggcagctga tcgatgacgt
      481 cgcggcgaag ctgcaggctg ctcgacacca gcgtctccac gcgcgcatcg ggcgccaccc
      541 cggagccgag catcaggttc tcggcatcga tctgcatgcc gtggccggtg tagtagaaaa
      601 gaatggtcgc gtcgggtggc gacgccgcag cgaccttcga aaatgcctgg atcgcggcct
      661 gcatctggcc cgggttctgg tcgagcgcgg aagtgacctt gaagccgcgc gcttgcagcg
      721 cgccgccgag atcgcgcacg ttcttgtaga ccggtggcag atcgaacggc gcgggatagg
      781 atcggttgcc gatcagcagg gcgaggcgcg tcgggctgtc gtctcctgcc gccgcggcgg
      841 atgccgccac ctggcccagc gcacccttgc cccaggccgc acccgcagca gcgaagggca
      901 gtgagacgaa ggctcggcga cgttcgttgg ggttcattcg cagcgcagcg tacccggctt
      961 caggtcgcac gtgtcgggca ggcggatcga ttcgcgtccg acggtggccg gccgcccggc
     1021 gccgacttcg gcgatgacgc ccgagcgcag tcgcgacgac acgttttccg tgcggacggc
     1081 ttcttcgaag cgctcgtgcc gtgcctcctc gatcagcgcg atgaccgcgg ccagcgcgcc
     1141 ggcttcctgc ggcgtgcgtg gcgcatcgcc gttgtagaag atgcgaattt ccgacagctt
     1201 gccgttttcg tcatagaagg gccggacgtc ggcgttgccg ccgaggaaga tcacggcgtt
     1261 ctcgccgccg actcccttcg gatcggctgt gacgaagccg ccttcggcgc cgcccacttg
     1321 cacccggatg tagttgtcgg aggccgggct gccgaaggcg ccattgcccg tggccagggc
     1381 cgtgggcgtg aggacgaagg taatgcccgg gatcgacgtc tgcgaaccct ggacgttgtt
     1441 gtagggcagc cgcgcccgga tcagcccgtc ggtgccggtc gcgagcacat tggcggtgag
     1501 cttgaccacg tcgccttcga tgcccgcggg cagcgtcgtc tggtcgccat ccgccggcgg
     1561 cgaaccggcg acatggatct cgctcgaggc gatgcggatg aagccgagcg tgagggtgct
     1621 ggcactgttg aagcggctga ggtcgttgtc gtcgagcttg ccgctgacag ccgagatgcc
     1681 gccgagcagc gtgttgccgg gctggtcgag ctgcaaggtg ccgccgttcg gcgatcgaaa
     1741 cgcgatcagg ctgccgctgg ccgaggaaag cgacgcgccg acatcctcga cgatcgttgc
     1801 gctcgcctcc ttgatcggca cgaagccgaa gttgaagggc ttgccctgga agtccgcatc
     1861 ggtgatgtcg atcgccgtcg gtgttgcgtt cgcggtgaac gaaagcgttc ctgcggcgag
     1921 ctgtacgcta ccggtgaggt ggagcacccc gtccgtgtcg acggcgccgc cgttggcgag
     1981 cgtgatgctt cccaggttca ggtcgagcga ggaatgcagt tgcagcgagt cggcattcgc
     2041 tgccaccggc gcggcgaact ggttcgtcgc ctgcatggcg gaaatcgcgc cgccgctgaa
     2101 gagaatgggc acgctcgccg tcaccggatc gccgtaggtt tgcgagccag tcgtgaagac
     2161 gcgggcgctg ccgctcccat ccgccgtgtc gaatagcgtt cgttcgccgc tggtgccgtc
     2221 ccagtcggcg gcggccggat tgttgtcggt cgtcagggat ttgagtggcg ccgtgactcc
     2281 gatcggtccg gaaattcggg tcgtcccgcc gctgtcgagg gccagggcga aggggccgtc
     2341 gatgctgccg ctgacagtga tgtcgccgct gcccgtctgc agcgtcgtgt cggcgcgcaa
     2401 ggtcaggttg ccggcgctga tggctccgct gccatcggcg cgtccgatca cgtgggtgcc
     2461 gaatccgatc gcaccgtcga gcaccgcctg ggagaccttc aacgcgccgg cgccgcccgc
     2521 gactccgacc gacagcgtcg catcgaccgg ggcgatgctg agcgtgccgc tgcccgagca
     2581 ggatgtgcgc ccgcggcttg gcgccgcgct cgacctggcg g
//
LOCUS       DASRWS010000311         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1280174, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000311 DASRWS010000000
VERSION     DASRWS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280174"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFF72_05795"
     CDS             <1..216
                     /locus_tag="VFF72_05795"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803763.1"
                     /note="lipoyl/octanoyltransferase; catalyzes the transfer
                     of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto
                     lipoate-dependent enzymes like pyruvate dehydrogenase and
                     the glycine cleavage system H protein; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase"
                     /protein_id="HZV92709.1"
                     /translation="KIAAIGVKVSRHRTYHGVALNVTMDLRPFDGIDPCGHAGLKTVD
                     LSTIGVRAAWDDVATTLGESLATTLGP"
     gene            213..1178
                     /gene="lipA"
                     /locus_tag="VFF72_05800"
     CDS             213..1178
                     /gene="lipA"
                     /locus_tag="VFF72_05800"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZV92710.1"
                     /translation="MMTPTPYDPTQKQKAKDKTARIPIKVVAAEALKKPDWIRVKAAS
                     PSTRFYEIKQILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGH
                     GRPDPLDAEEPLHLAQTIAALRLKYVVITSVDRDDLRDGGAAHFVACIEKTRELSPAT
                     KIEILTPDFRGRMDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYAYSLNLLRRF
                     KEFAPDVPTKSGLMVGLGETDDEILQVMRDMREHGIDMLTIGQYLAPSGHHLPVRRYV
                     HPDTFRMFEREAAAMGFRHAAVGALVRSSYHADQQAHAAGVAEAA"
     gene            1185..2177
                     /locus_tag="VFF72_05805"
     CDS             1185..2177
                     /locus_tag="VFF72_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92711.1"
                     /translation="MTPDGEGPGSGADPVLACGNCHAPMQRLALCGHYGSKVELDLCD
                     SCDLVWFDDVETARLNGPGMLELIDRMARAYDLPYETLRDAVTCPRCAATLVLAHNQS
                     RWGRSVQLQCPRGHGDYQSFAEFLEEKGLLRPMSRIDRSRLLRDKGRIDCVNCGASVG
                     LDDERCPYCHSIPSLLDVARLARALDPEQMLGAEPLPGARATQEAMQCAACGAALPPG
                     ETMSCAQCGATLAIASLREANAAVQALAPALRANAVRPPPQVVARRLAAIDADLPRQR
                     EWAAGMERDARHGSQESNDEDFEWSSLFRRGTNPVRAVLIAFALWYLWAYWHRG"
     gene            2209..>2745
                     /locus_tag="VFF72_05810"
     CDS             2209..>2745
                     /locus_tag="VFF72_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZV92712.1"
                     /translation="MSALLPALGAIALWSTLASFGVALARVPPFLLTGLALLVGSLPA
                     WPLARHWRVSASTLLLGVCGLFGFHFLLFVALRTAPPVEANLVNYLWPLLIVVLAPVV
                     LPGLSLRPVHVVAALTGFAGAAMAILGGHGLDHGFAWGYVAALGSALIWALYSLLTRR
                     VADFPTAAVGLFALVSGLL"
BASE COUNT          399 a          958 c          926 g          462 t
ORIGIN      
        1 aagattgccg cgatcggcgt caaggtcagc cggcaccgca cgtaccatgg cgtcgccctc
       61 aacgtgacga tggacctgcg acccttcgac ggcatcgacc cctgcggaca cgccggcctg
      121 aagacggtcg atctttctac aatcggcgtt cgtgctgcgt gggacgacgt ggcgacgacg
      181 ctcggcgaga gtctcgccac caccctcggc ccatgatgac cccgacgccc tacgatccga
      241 cgcaaaagca gaaggcgaaa gacaagacgg cgcgcatccc gatcaaggtg gtcgccgccg
      301 aagcgctgaa gaagcccgac tggatccgcg tcaaggcggc gtcgccgtcg acgcgcttct
      361 acgagatcaa gcagatcctg cgcgagcaca acctgcatac ggtgtgcgag gaagcgagct
      421 gcccgaacat cggcgaatgc ttcggcaagg gcaccgcgac gttcatgatc atgggcgaca
      481 agtgcacgcg ccgctgcccg ttctgcgacg tcggccacgg ccggcccgac ccgctcgatg
      541 cagaagagcc gctgcacctg gcgcagacca tcgccgcgct gcggctgaag tacgtcgtga
      601 tcaccagcgt cgaccgcgac gacctgcgcg atggcggcgc cgcgcacttc gtggcctgca
      661 tcgagaagac ccgcgagctg tcgccggcga ccaagatcga gatcctgacg cccgactttc
      721 gcggccgcat ggatcgggcc ctcgagatcc tcaaggcggc gccgcccgac gtgatgaacc
      781 acaacctcga gacggccccg cgcctctaca aggaagcgcg gccgggctcc gactacgcgt
      841 attcgctgaa ccttctcagg cggttcaagg agttcgcgcc ggacgtgccg accaagagcg
      901 gcctgatggt cgggctgggc gagaccgacg acgagatcct gcaggtcatg cgcgacatgc
      961 gcgagcacgg catcgacatg ctgacgatcg gtcagtacct cgccccgagc gggcatcacc
     1021 tgccggtgcg ccgctacgtc caccccgaca ccttccggat gttcgagcgc gaggctgcgg
     1081 cgatgggctt ccgtcacgcc gcggtcggcg cgctggtgcg ctcgagttat cacgctgacc
     1141 agcaggcgca tgccgcgggt gtcgccgagg cggcctgagg ccggatgacg ccggacggcg
     1201 aaggccctgg cagcggcgcc gatccggtgc tcgcctgcgg caattgccac gcgccgatgc
     1261 agcggctcgc gctctgcggc cattacggct cgaaagtcga actcgatctt tgcgacagtt
     1321 gcgacctcgt ctggttcgac gacgtcgaga cggcccgcct gaacgggccc ggcatgctcg
     1381 agttgatcga tcgcatggcc cgcgcctacg acctgcctta tgagacgctg cgcgatgccg
     1441 tgacctgccc gcgctgtgcg gcgacgctgg tgttggcgca caaccagtcg cgttggggcc
     1501 gttcggtgca gttgcagtgc ccgcgtggcc acggcgacta tcagtcgttc gccgagtttc
     1561 tcgaggaaaa gggcctgctg cggccgatgt cccggatcga tcgctcgcgc ctgctgcgcg
     1621 acaaagggcg gatcgattgc gtgaactgcg gcgcgtcggt cggtctcgac gacgagcggt
     1681 gcccttactg ccactcgata ccgagcctgc ttgacgtcgc tcggttggcg cgcgcgctcg
     1741 atccggagca gatgctgggc gccgagccgc tgcccggcgc ccgcgccaca caggaagcga
     1801 tgcagtgcgc tgcctgcggc gcggcactgc cgcccggcga gacgatgagt tgcgcgcagt
     1861 gcggggcgac gctggccatt gcgagcctgc gcgaagcgaa cgccgcggtc caggccttgg
     1921 caccggcctt gcgcgccaac gccgtccggc cgccgccgca ggtcgtcgcc cgtcgcctcg
     1981 ccgccatcga tgccgatctg ccgcggcaac gcgaatgggc cgccggaatg gaacgcgacg
     2041 cgcgtcatgg atcgcaggag tcgaacgacg aggatttcga atggtcgtcg ctttttcggc
     2101 gcggcacgaa cccggttcgc gccgtgctga tcgccttcgc tctctggtac ctttgggcgt
     2161 actggcatcg cggctgacag gcggacgacg acggcacgct gcccggcaat gagtgcgctg
     2221 ttgccggcac tgggtgcgat cgcgctgtgg tcgacgctcg cttcgttcgg cgtcgccctg
     2281 gcacgggttc cgccctttct tctcaccggc ctggcgctgc tggtcggcag cctgccggca
     2341 tggccgctgg cgcgccactg gcgggtctcc gcctcgacgc tgctcctggg cgtctgcggc
     2401 ctgttcgggt ttcatttcct gcttttcgtg gcgctgcgca cggcgccgcc ggtcgaggcc
     2461 aacctcgtca actacctgtg gccgctcctg atcgtcgttc tcgcgcccgt cgtcctgccg
     2521 ggtctctcgc tgcgtccggt acacgtcgtc gcggcgctga ccggattcgc gggcgcggcg
     2581 atggcgatcc tcggcggaca cggtctcgac cacgggttcg cctggggcta tgtcgctgcg
     2641 ctcgggtcgg cgttgatctg ggccctctac tcgctcctga cgcgtcgcgt cgccgacttc
     2701 ccgaccgccg cggtcggtct tttcgcgctc gtctccgggc tcctt
//
LOCUS       DASRWS010000312         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2146248, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000312 DASRWS010000000
VERSION     DASRWS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146248"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFF72_05815"
     CDS             complement(<1..625)
                     /locus_tag="VFF72_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HZV92713.1"
                     /translation="MKSRGQALRDYVVRRLLLMIPTFIGITFVTFLLCQFVPGGQIDQ
                     MRMRLAGAEGGEMSSGGGRAQLEIPDAQLAQLRAYYGFDKPLPVAYVDWLGKALRLNF
                     GESFRYNVPVTQVIASRLPVSIYYGLITALFTYGICIPLGTLKAIRHRTRLDTATSVL
                     IFLGYAIPGFALGAVLSNLLAVHWAIFPLGGFQSAGAARLGFWAHAAD"
     gene            complement(838..1170)
                     /locus_tag="VFF72_05820"
     CDS             complement(838..1170)
                     /locus_tag="VFF72_05820"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HZV92714.1"
                     /translation="MGLLDGLLGGVVGGEMVSVVNGLIERHGGVQGIVQQLQSQGLGP
                     TVQSWIGNGPNQGISPDQVHQTFGAETINALAAKAGLSPQELASKLSAILPQAIDRLT
                     PGGVVPAQ"
     gene            complement(1239..2555)
                     /locus_tag="VFF72_05825"
     CDS             complement(1239..2555)
                     /locus_tag="VFF72_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZV92715.1"
                     /translation="MSWYAALLVMLGPLCGLLLLGLPVAFAFLLVDIVGALIFLGGDA
                     GLLQMVRNAVTALTNYSLAPIPLFILMGELLLHSGVAFRAINAIEKLIAKVPGRLPIV
                     SVVGGTIFAALSGSSLANTAMLGSALLPDMLKRGYEPRIAMGPIMATGGIAMLIPPSA
                     LAVLLGSLAGVSISKLLIAGIVPALLMAALFIGYIVAVCVLQPGRAPRDEGSTEHGWA
                     RWRPFVVDVLPLSLVFVAVIGSLVGGIATPTESAALGCVAAAVAALGYRSLDWRKLVI
                     SFRETARVSVMTLFIIAASVTFAQILAFSGATDGLVSVVRQAGLPPFGLALGMVAILL
                     FLGCFVDQVSMLMITIPLFIPMAQTAGIDLLWLGVLYLLTMEISFLTPPFGLLLFVMK
                     GVAPPQITLAQVYRAALPFLGLELLVLALLLVWPAIGTWLPEMLVR"
BASE COUNT          477 a          852 c          918 g          356 t
ORIGIN      
        1 tgtcggccgc gtgcgcccag aagccgaggc gcgccgcgcc tgccgactgg aagccgccca
       61 gcggaaagat agcccagtgc acggccagca ggttgctgag cacggcgccc agggcgaagc
      121 cggggatggc gtagccgagg aagatcagca ccgaagtcgc ggtgtcgaga cgggtgcggt
      181 ggcggatcgc cttcagcgtg cccagcggaa tgcagattcc gtaggtgaac aaggcggtga
      241 tcaggccgta gtagatcgac accggcaagc ggctggcgat gacctgcgtc accggcacgt
      301 tgtagcggaa cgactcgccg aagttcagtc gcagcgcctt gcccagccag tcgacatagg
      361 ccaccggcaa gggcttgtcg aacccgtagt aggcgcgaag ctgggcgagc tgcgcgtcgg
      421 ggatctcgag ctgggcccgg ccaccgccgc tgctcatctc gccgccttcg gcgccggcga
      481 gccgcatgcg catctggtcg atctggccgc cgggcacgaa ctggcagagc aggaaggtga
      541 cgaaggtgat gccgatgaag gtcgggatca tcagcagcag ccggcgcacg acgtagtcgc
      601 gcagggcctg accgcgagat ttcactcgag cgagccttcg gcaaacgagg tcgcgccgtc
      661 catggtccgg gtcctgcagg gcttgcagga cttgtcgagc gtctgctgcc gggtcgtctt
      721 ggtcacgtcg acttccttgt cttctccgtc tcgcctcgac attgtgccgt cccggacagg
      781 gcggagcccc gacggcgcgt cgaccgggtc attcgaagcg cgccgcgaag cgctcgttca
      841 ttgcgccggg acgacgcctc ccggcgtgag cctgtcgatc gcctgcggca ggattgccga
      901 cagcttgctc gccagctcct gcggcgacag gcccgccttc gccgccagcg cattgatcgt
      961 ctcggcgccg aaggtctggt gcacctggtc gggcgagatg ccctggttcg ggccgttgcc
     1021 gatccacgac tgcacggtcg ggccgaggcc ctggctctgc aattgctgga cgatgccctg
     1081 cacgccgccg tgtcgttcga tcagaccgtt gacgacggag accatctcgc caccgacgac
     1141 gccacccaga agtccgtcaa gcagtcccat gtgttcgctc ctgtgaaacg ttggaggatg
     1201 ccacgttcga cgggcggctg gccggccgtt cctgacggct acctgacgag catttccggc
     1261 agccaggtgc cgatcgcggg ccagaccagc agcaaggcca gcacgagcag ttccagcccg
     1321 aggaagggca gcgctgcgcg atacacctgg gcgagcgtga tctgcggcgg cgccacgccc
     1381 ttcatcacga agagcagcaa gccaaaaggc ggcgtcagga agctgatctc catggtcagc
     1441 aggtacagca cgccgagcca gagcaggtcg atgccggcgg tttgcgccat cgggatgaag
     1501 agcgggatcg tgatcatcag catgctcacc tgatcgacga agcagccgag gaacagcagg
     1561 atcgcgacca tccccagggc caggccgaac ggcggaaggc cggcttgccg gacgaccgag
     1621 acgaggccgt cggtcgcgcc cgagaacgcc aggatctggg cgaaggtgac cgaagcggcg
     1681 atgatgaaga gcgtcatgac gctgacgcgc gccgtctcgc gaaacgagat caccagcttg
     1741 cgccaatcga gcgagcggta gccgagcgcg gcgaccgccg ccgcgacgca gccgagcgcg
     1801 gccgactctg tcggcgtggc gatgccgccg acgagactgc cgatgacggc gacgaagacg
     1861 agcgacagcg gcagcacgtc gacgacgaac ggtcgccagc gtgcccagcc gtgttcggtc
     1921 gaaccctcgt cgcgtggcgc ccggccgggc tggaggacgc agacggcgac gatgtagccg
     1981 atgaagagcg ccgccatcag caacgccggg acgatgcccg cgatgagcag cttcgagatc
     2041 gacacgccgg cgaggctgcc caggagcacc gccagcgccg acggcgggat cagcatggcg
     2101 atgccgccgg tggccatgat cggccccatg gcgatgcgcg gctcgtagcc gcgcttgagc
     2161 atgtcgggca ggagcgcgct gccgagcatc gccgtgttgg cgagcgacga tcccgacagc
     2221 gccgcgaaga tggtgccgcc gacgacgctg acgatcggca ggcgtcccgg caccttggcg
     2281 atcagcttct cgatcgcatt gatggcccga aaggcaacgc cgctgtgcag cagcagctcg
     2341 cccatcagga tgaagagcgg aatcggcgcc aatgaatagt tggtgagcgc ggtgaccgcg
     2401 ttgcggacca tctgcagcaa gccggcatcg ccacccagga agatgagcgc accgacgatg
     2461 tcgaccagca ggaatgcgaa ggcgacgggc aggccgagca gcagcaggcc gcagagcggc
     2521 cccagcatga ccagcagcgc ggcgtaccag ctcatcgttc gggcgacgtc gccctacagg
     2581 tcggccggcg ccggcgcgtc gcc
//
LOCUS       DASRWS010000313         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1576852, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000313 DASRWS010000000
VERSION     DASRWS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576852"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1480)
                     /locus_tag="VFF72_05830"
     CDS             complement(134..1480)
                     /locus_tag="VFF72_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92716.1"
                     /translation="MTIPLEADDPDPAGQRAEPDRIRRRVVQVAGTVLAAATLPAGLA
                     RAATGDSRALKVGFISPRTGPLAGFGECDPHVLQLARKALATGWKVDGKTYAVEILDR
                     DSQSDPARASQLANSLINEARIDMMLVTSTPEVVNPVSDACEAAGMPCLSTVVPWESW
                     YFGRGAKPGQPSPFKWTYHFCFGGHDFFVAYNSLWNGPVKTNKRVGILYPNDADGNAV
                     REHLAPALAKAGFTIIDSGAYEDGTTDFSAQIAKFKAEQCEIFNTFPIPPDFAAFWRQ
                     AAQQGYTKMVKIAQIAKTGLFPSQVEALGSLGYKLATACYWHPTWPYKSPLTGLSSQA
                     LSDGYEKATGRQWTQQVGASMALLDAGFAALSNSGNPKVKADVARSMSTLKVVTPVGP
                     VDFTAGPVPNVSTTPIIGSQWVKAKAGSRFKLALPLTEHADDPHVPIGAQLQPYNA"
     gene            complement(1600..2097)
                     /locus_tag="VFF72_05835"
     CDS             complement(1600..2097)
                     /locus_tag="VFF72_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92717.1"
                     /translation="MKTHWRTLWQQRDIVVYRDEVEVDRLAADRIARVLLVYRGHGDF
                     PGDIATSIVELAGDPGGFVLFDACTGFAGRVNFERQAYWQERACVYWVPAPSAALPWR
                     LRLGGWRGEAATRAFRRIGRSELEGCIAGWVLEGPQIWEERKQSRLERSRPFGAASTG
                     WRAAA"
     gene            complement(2160..>2973)
                     /locus_tag="VFF72_05840"
     CDS             complement(2160..>2973)
                     /locus_tag="VFF72_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZV92718.1"
                     /translation="LKLTDESAKHIGRLARLESLNLQYTGLTDAGLRRAYQFWTSRGI
                     AIVDVNYGGSSGYGRAYRERLKGRWGIVDVDDCVAAARFLAARGDVDARRLAIRGHSA
                     GGFTTLAALTFRDCFQAGASHYGISDLEALARDTHKFESRYLDSLIGPYPARADLYRA
                     RSPIHAAGRLSSALILLQGAEDKVVPPGQAESMFEAVRAKGLPVALLVFAGEQHGFRQ
                     AAHIVRALEAELYFYGRVFGFVPADTIEPMPIENLPTERTFRLDTTRIDEQR"
BASE COUNT          447 a          992 c         1065 g          469 t
ORIGIN      
        1 cgtcttgccg gctccgttcg ggccgacgat gcccacggcc tcgtcggcgg cgacggcgaa
       61 gtcgatccgc tcgatcgcgg ccagcgcacc gaatttcttg ccgatgccgt cagccgcgag
      121 gatcgtcacc gcgtcaggcg ttgtagggct gcagctgcgc tccgatcgga acgtgcgggt
      181 cgtcggcgtg ctcggtcagc ggcagcgcca gcttgaaacg gctgccggcc ttcgccttga
      241 cccactggct gccgatgatc ggcgtggtcg agacattcgg caccggcccg gccgtgaaat
      301 cgaccggccc gaccggcgtc accaccttga gcgtgctcat cgacctggcg acgtcggcct
      361 tgaccttcgg gttgccgctg ttcgacaagg cggcgaagcc ggcgtcgagc agcgccatgc
      421 tggcgccgac ctgctgcgtc cactggcgtc ccgtggcctt ctcgtagccg tcgctcaacg
      481 cctgcgacga caaccccgtc aacggcgact tgtacggcca tgtcgggtgc cagtagcagg
      541 cggtcgcgag cttgtagccc agcgagccga gcgcttcgac ctgcgacggg aagaggcccg
      601 tcttggcgat ctgcgcgatc ttcaccatct tcgtgtagcc ctgctgcgcc gcctggcgcc
      661 agaaggcggc gaagtcgggc gggatcggga aggtgttgaa gatctcgcac tgctcggcct
      721 tgaacttggc gatctgcgcc gagaagtcgg tggtgccgtc ttcgtacgcg cccgaatcga
      781 tgatcgtgaa gccggccttg gccagcgccg gagcgagatg ctcgcgcacg gcgttgccgt
      841 cggcgtcgtt ggggtagagg atgccgaccc gcttgttggt cttgaccggg ccgttccaca
      901 gcgagttgta ggcgacgaag aagtcgtggc cgccgaagca gaagtgatac gtccatttga
      961 aaggcgacgg ctggcccggc ttggcgccgc ggccgaaata ccaggactcc cacggcacga
     1021 ccgtcgacag gcacggcatg cccgcggcct cgcaggcgtc cgacaccggg ttgacgacct
     1081 ccggcgtcga ggtgaccagc atcatgtcga tgcgggcctc gttgatgagg ctgttggcga
     1141 gctggctggc gcgtgccgga tcggactgcg agtcgcggtc gaggatctcc accgcatagg
     1201 tcttgccatc caccttccaa cccgtggcca gcgcctttcg agccagttgc aggacgtggg
     1261 ggtcgcactc gccgaagccc gccagcgggc cggtgcgcgg gctgatgaac ccgaccttga
     1321 gcgccctcga atcgcccgtt gcggcgcgcg ccaggccggc cggcagcgtc gcggcggcga
     1381 gaacggtgcc cgccacctgc acgacgcggc ggcgaatgcg atcgggttcg gcgcgttgcc
     1441 ctgccgggtc cgggtcgtct gcttccaatg ggatggtcat ggcgtctcct tcaaagatcg
     1501 aggaatacca tgacggggct cacagcacga taagtagtgc tcccgatgac agggtgatgc
     1561 cggctgcgac caccggcttc ggacaacctt cggcgcgcgt caggcggcgg cgcgccagcc
     1621 ggtcgaggcg gcgccgaaag gccggctgcg ctccaggcgg ctttgctttc gctcttccca
     1681 gatctgcggt ccctcgagaa cccacccggc gatgcagccc tcgagctcgg agcggccgat
     1741 gcggcgaaag gcccgcgtcg ccgcttcgcc gcgccagccg cccaggcgca gccgccaagg
     1801 cagtgccgcg ctcggcgccg gcacccaata gacgcaggcg cgctcctgcc agtacgcctg
     1861 gcgttcgaaa ttgacgcggc cggcgaaacc ggtgcaggcg tcgaacagca cgaacccgcc
     1921 cgggtcgccc gcaagctcga cgatgctggt cgcgatgtcg cccggaaagt cgccgtggcc
     1981 ccggtagacg aggaggacgc gggcgatccg gtcggcggcc agccggtcga cctcgacctc
     2041 gtcgcggtag acgacgatgt cgcgttgttg ccagagggtt cgccagtggg tcttcatggc
     2101 cggtcctttg cggcacgcca tcggcttgcg tgccgtgatg aaagcaggga cctgcgacct
     2161 tagcgctgct cgtcgatccg agttgtgtcg aggcgaaacg tccgctccgt cggcaggttc
     2221 tcgatcggca tcggctcgat cgtgtcggcc ggcacgaagc cgaagacgcg gccgtagaag
     2281 tacagctcgg cctcgagcgc acggacgatg tgggcggcct ggcgaaagcc atgctgctcg
     2341 cccgcgaaga cgagcagggc caccggcagc cccttcgcgc gaaccgcctc gaacatcgac
     2401 tcggcctggc ctggcggcac gaccttgtcc tcggcgccct ggagcaggat cagcgcgctc
     2461 gacagccgcc cggcggcatg gatcggcgag cgggcgcggt agagatcggc gcgggccggg
     2521 taggggccga tcagcgaatc gaggtagcgc gattcgaact tgtgggtgtc gcgggcgagt
     2581 gcctcgaggt cgctgatgcc gtagtggctg gcgccggcct ggaaacagtc gcgaaaggtg
     2641 agggcggcca gcgtcgtgaa gccgccggcg ctgtggccgc ggatcgccag ccgccgcgca
     2701 tcgacgtcgc cgcgcgcggc gagaaagcgc gccgcggcga cgcagtcgtc gacgtcgacg
     2761 atgccccagc ggcccttgag ccgctcgcgg taggcgcggc cgtagccgct cgagccgccg
     2821 tagttgacgt cgacgatggc gatgccgcgg ctggtccaga actgataggc gcggcgcagg
     2881 ccggcgtcgg tgaggccggt gtactggagg ttcagcgatt cgagccgcgc cagccggccg
     2941 atgtgcttcg ccgactcgtc ggtcagcttc aga
//
LOCUS       DASRWS010000314         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3007895, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000314 DASRWS010000000
VERSION     DASRWS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007895"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="bamC"
                     /locus_tag="VFF72_05845"
     CDS             <1..890
                     /gene="bamC"
                     /locus_tag="VFF72_05845"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HZV92719.1"
                     /translation="AAIAPSSIGDFHMERQGDERWLRTSLPPEQVYPQVRAFWKDNGF
                     ALTEDRPEAGVLETDWAENRAKLPLDYLRRTIGKALDGLYSTSELDKFRTRIERTPSG
                     GSDIFITHRGMAEVYTGPRHDGTIWQPRPVDPQLEAEFLSRLMVKLGAKEETAKAQLA
                     AVSTAPVAPPHARMLADRPGPTLQLDDNFDRAWRRVGIALDRSGFTVEDRDRTQGVYF
                     VRYVDPKFAGREEPNFFSRLFSFGHKKAEDGSPVKYRVKVTGENATSSTVSVEDLQGK
                     PENSDNGKRIARLLLEDLR"
     gene            928..1707
                     /locus_tag="VFF72_05850"
     CDS             928..1707
                     /locus_tag="VFF72_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV92720.1"
                     /translation="MRFRSLGSGSGGNATVVEASAGITTTRLLVDAGFSLRELDTRLA
                     RAGLAGDDLDAIFITHEHGDHIGCAVALATRHGLPLWMSRGTWRAIGEPVAPPGLRFA
                     RDDELLRIGDLEVLPFTVAHDAAEPLQLRCGDGAARLVVLTDLGSITAHMIERVERCD
                     AIVLECNHDAAMLAASRYPASLKARIGGRFGHLSNETATQILRRCASARLRHVVAAHL
                     SRENNRPDIARAALACSWGGPNGRDIVIADPVTGCDWLQIA"
     gene            1962..2378
                     /locus_tag="VFF72_05855"
     CDS             1962..2378
                     /locus_tag="VFF72_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92721.1"
                     /translation="MADDAAIIPDVAAAEAESIADEAAIMLDVAAAEAESIADEAASI
                     ADEAGAIVAGGVAVVVVVVVDVSSFLLQAAKEIAAASETMSRAVFIFLLDFQVVRTIS
                     GNCGNPSARTPSFRRHGMQGHLPCLTAHYRHLKGFP"
     gene            complement(2375..2863)
                     /locus_tag="VFF72_05860"
     CDS             complement(2375..2863)
                     /locus_tag="VFF72_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92722.1"
                     /translation="MNDESVTRIESRNEFRAAVGTALAKAAQDGASEICMIDPRFVDW
                     PLNDPAIVELLSRWVSSRRRCLVMAHGFDDLVRAAPRFVEWRRQWSHVVQCRTTEEIE
                     ADQVPTLLWVREMACLKLLDRVRYGGTFSGREIDLVETRETIDALLQRSVEAFPATTL
                     GL"
     gene            complement(2860..3996)
                     /locus_tag="VFF72_05865"
     CDS             complement(2860..3996)
                     /locus_tag="VFF72_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZV92723.1"
                     /translation="METDRPLALLGGLSASTFMRTHWQARPLLVRSALRDVDLHIPDR
                     SRLFELAARDDVESRLVIREGERWSVRHGPFRRRALPPLTQHGWTLLVQGVDLHDDAA
                     HDLLARFRFISDARLDDAMFSYASDGGGVGPHVDSYDVFLLQTSGRRRWRIGRARRAR
                     LRDDVPLKILRDFVPSHDWLLEPGDMLYLPPGWAHEGTAQGPCITCSIGFRAPATNLL
                     AADVLQRLAETRVDDGAQGELYGDRGQAATVHPGRIPAGLQRFADAAVGMALRDREAR
                     RRALGEVLSEPKRDVWFEAQPAAATGRAVVLDRRSRMLYDARHVFINGEAYRAGGGDA
                     TLMRRLADARRLCARDVQGLSRPARRLLGRWVQAGWCVEIEEGP"
     gene            4021..4542
                     /locus_tag="VFF72_05870"
     CDS             4021..4542
                     /locus_tag="VFF72_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZV92724.1"
                     /translation="MQIAAPCVVTLTWRLVDAQGGEIDELVDPIEFFFGGDDLLAKVE
                     EALAGHEAGYETTLHLEPEHAFGDYDPQLVFFEARNILPEGIEPGMQFEGPPPGAQTP
                     GLSAEAIYTVTEVYPDHVVLDGNHPLAGIALRLDIKVRDVRAATDAEIEAGSVGGSAV
                     SVLATAPSGTRLH"
     gene            complement(4588..5052)
                     /gene="dut"
                     /locus_tag="VFF72_05875"
     CDS             complement(4588..5052)
                     /gene="dut"
                     /locus_tag="VFF72_05875"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZV92725.1"
                     /translation="MTPGPVADIDVRVLDPRVADQLPSYATAGSAGLDLRACIDAPMT
                     LAPGTARLIATGLAIHIADPSLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVS
                     CWNRGTSPYTVQPLERIAQLVIVPVVQARFHVVESFEDSARGGGGFGSTGRA"
     gene            complement(5049..>5456)
                     /locus_tag="VFF72_05880"
     CDS             complement(5049..>5456)
                     /locus_tag="VFF72_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HZV92726.1"
                     /translation="DVFVATAAVADWRAAEVSAAKIKKTTDRRVPALALVENPDILAA
                     VAALAKPPYCVGFAAESDNLLAHARDKMKRKKVPLIVANIGADTFGEDDNAITLVDAG
                     GEQAWPRAGKLALARRLVAEIATRLGSAARSLG"
BASE COUNT          917 a         1916 c         1806 g          817 t
ORIGIN      
        1 cggccgcgat cgcgccttcg tcgatcggtg atttccacat ggagcgccag ggcgacgagc
       61 gctggctgcg caccagcctg cctccggagc aggtctaccc gcaggtgcgt gcattctgga
      121 aggacaacgg cttcgccctc accgaggacc gacccgaagc gggcgtgctc gaaaccgact
      181 gggcggaaaa ccgcgccaag ctgccgctcg actatctgcg ccggacgatc ggcaaggccc
      241 tcgacggcct gtattcgacc agcgagctcg acaagttccg gacccggatc gaacggactc
      301 cgtccggcgg ctccgacatc ttcatcaccc atcgcggcat ggccgaggtc tacaccgggc
      361 cgcgccacga cggcacgatc tggcaaccac gcccggtcga cccgcaactc gaggcggaat
      421 tcctgtcgcg gctgatggtc aagctgggtg ccaaggagga gacggcgaag gcgcagctcg
      481 ccgccgtatc gacggctccc gtcgcgccgc ctcatgcccg catgctggcc gaccggccgg
      541 gcccgacgct gcagctcgac gacaacttcg atcgcgcctg gcgtcgcgtc ggcatcgccc
      601 tcgaccgcag cggcttcacc gtcgaggacc gcgaccggac ccagggcgtc tacttcgtgc
      661 gctacgtcga cccgaagttc gccggccgcg aggaacccaa cttcttcagc cgcctgttca
      721 gcttcggcca caagaaggcc gaagacggtt ccccggtgaa ataccgcgtc aaggtcaccg
      781 gcgaaaacgc gaccagcagc acggtctcgg tcgaggacct gcagggcaag cccgaaaaca
      841 gcgacaacgg caaacgcatc gccaggctgc ttctagaaga cctgcggtga gctcgcggct
      901 cgacgcctcg gcgcggtcgt cagggtgatc cgctttcgca gcctcggcag cggcagcggc
      961 ggcaacgcga cggttgtcga ggccagcgcc ggcatcacga caacgcgcct gctcgtcgat
     1021 gccggcttct cgctgcgcga gctcgacacc cggctggccc gggccggcct cgctggagac
     1081 gacctcgacg ccatcttcat cacccacgag cacggcgatc acatcggctg cgcagtggcc
     1141 ctggcgacgc gtcacggctt gccgctgtgg atgagccgcg gcacctggcg cgccatcggc
     1201 gagccggttg cgccgcccgg gttgcgcttc gcgcgtgacg acgagctgct tcgcatcggc
     1261 gacctcgagg tgctgccctt caccgtcgcg cacgacgccg ccgagccgct gcaacttcgc
     1321 tgcggcgacg gcgccgcccg gctggtcgtc ctcaccgacc tcggctcgat cacggcgcac
     1381 atgatcgaac gcgtcgaacg atgcgacgcg atcgtcctcg agtgcaatca cgatgcggcg
     1441 atgctcgccg catcgcgcta cccggcgtcg ctgaaggcac gcatcggcgg ccgcttcggg
     1501 cacctcagca acgagacggc gacgcagatc cttcgtcgat gcgccagcgc gagattgcgc
     1561 catgtggtcg cggcgcacct gagccgtgaa aacaaccggc ccgatatcgc ccgcgccgcg
     1621 ctggcttgca gctggggcgg cccgaatggc cgcgatatcg tgatcgccga cccggtcacc
     1681 ggctgcgact ggctgcaaat cgcctgaccc gcaaggcggc cggaccaccg gcccggtgtg
     1741 ccgcattcgg cgcaggagct gcgtgtaaaa gctgccggcg cggcaggttt cgtgcatcga
     1801 agcctggctt gcgggactcg aaatcgacgg tggggccgtc ctcggcggcg cgtcgacgac
     1861 ttccgcttcc atcagcccgc gagggcaagc aaaaggccgc ccggaggcgg cctttgcacg
     1921 gagcgcaggc tccttacttc atcgcgccgg aagcggcttc catggccgac gacgcggcca
     1981 tcattcccga tgtggcggca gccgaggccg agtccatcgc cgacgaagcg gccatcatgc
     2041 tggatgtggc agcagccgag gcggagtcca tcgccgacga ggcggcatcc atcgccgacg
     2101 aagccggagc catcgtcgcg gggggcgtcg ccgtggtggt ggtcgtcgtg gtcgacgtgt
     2161 cttccttctt gctgcaggca gcgaaggaaa tcgcggcggc gagcgaaacc atgagcagag
     2221 ctgttttcat atttctcctc gattttcagg tggttcgaac aatttccggc aattgtggga
     2281 acccctctgc gaggacccca tcgttcagaa gacatggaat gcaggggcat ttaccgtgcc
     2341 taactgcgca ttataggcac cttaagggct ttccctagag tccgagcgtc gtcgctggaa
     2401 aggcctcgac agagcgttgc aaaagcgcat caatcgtttc ccgtgtctcg accagatcga
     2461 tttcccggcc cgagaacgtg ccgccgtaac ggacgcgatc gagcagtttc aggcaagcca
     2521 tttcccgcac ccagagcaac gtgggaacct gatccgcctc gatctcttcg gtggtccggc
     2581 attgcacgac atgagaccac tggcgacgcc attcgacgaa acgcggagcc gctcggacga
     2641 ggtcgtcgaa gccatgcgcc atgacgaggc agcgacgcct ggacgacacc cagcgcgaca
     2701 gcaactcgac gatcgcaggg tcgttcaacg gccagtcgac gaagcgcggg tcgatcatgc
     2761 agatctccga tgcgccgtcc tgcgccgcct tggccagcgc cgtgcccacg gcagcgcgaa
     2821 attcgttccg tgattcgatc cgggtcacgc tctcgtcgtt catggtcctt cctcgatttc
     2881 cacacaccaa cctgcctgca cccagcgccc cagcaaccga cgcgccggac ggctcagccc
     2941 ttgaacatcg cgtgcgcaca gacggcgcgc atcggcgagt cgacgcatca gtgtcgcatc
     3001 gccaccgccg gcgcggtagg cctcgccgtt gatgaacacg tgccgcgcgt cgtagagcat
     3061 gcgagagcga cgatcgagga cgaccgcgcg tccggtcgcg gcggcgggct gcgcctcgaa
     3121 ccagacgtcg cgcttgggct cgctcagcac ctcgccgagg gcgcgacgtc gagcttcgcg
     3181 gtcgcgcagc gccatgccga cggccgcatc ggcgaagcgc tgcaagccgg cggggatgcg
     3241 tccgggatgc acggtcgccg cttgtccgcg gtcgccataa agctcgcctt gcgcgccgtc
     3301 gtcgacccgc gtctcggcca ggcgctgcaa cacgtcggca gcgagaaggt tcgtcgccgg
     3361 cgcgcgaaag ccgatcgagc acgtgatgca cggcccttgc gcggttccct cgtgggccca
     3421 gcccggcggc aggtaaagca tgtcgcccgg ctcgaggagc cagtcgtggc tcggtacgaa
     3481 gtcgcgcagg atcttgagcg gcacgtcgtc acgcagccgg gcacgacggg cgcgaccgat
     3541 ccgccagcgc cgtcgcccgc tcgtctggag caggaagaca tcgtaggagt cgacgtgcgg
     3601 cccgacaccg ccgccgtcgc tcgcatacga gaacatcgcg tcatcgagac gagcgtcgga
     3661 tatgaagcga aagcgcgcga gcaggtcgtg cgccgcgtcg tcgtggaggt cgaccccctg
     3721 aaccagcagc gtccagccgt gttgcgtcag cggcggcagc gcgcgacgcc ggaagggccc
     3781 gtgccgcacc gaccagcgtt caccctcgcg aatgaccagg cgcgactcga catcgtcgcg
     3841 cgcggcaagc tcgaacaatc gcgaccgatc cggaatgtgg aggtccacgt cgcgcagggc
     3901 actgcgaacc agcagcggcc ttgcctgcca gtgcgtgcgc atgaacgtcg acgcggacaa
     3961 gccgccgagc agcgccaacg gacgatcggt ctccatgggt cattgtgcga tgatcgttcg
     4021 atgcagatcg ccgcaccttg cgtcgtcacc ctcacctggc gcctggtcga tgcgcaaggt
     4081 ggcgaaatcg acgagctcgt cgacccgatc gaattcttct tcggcggcga cgacctcctc
     4141 gccaaggtcg aggaagcgct ggccgggcac gaggccggct atgaaacgac gctgcacctc
     4201 gagcccgagc atgccttcgg cgactacgac ccgcaactgg tcttcttcga ggcgcgcaac
     4261 atccttcccg aaggcatcga gccgggcatg cagttcgagg ggccgccccc cggcgcccag
     4321 acacccggcc tgtcggccga ggccatctat accgtgaccg aggtctatcc ggaccacgtc
     4381 gtcctcgacg gcaaccatcc cctcgccgga atcgcccttc gcctcgacat caaggtgcgc
     4441 gacgtccgcg cggccaccga cgccgagatc gaggccggca gcgtcggcgg ctcggccgtc
     4501 agcgtgctcg cgacggcgcc atccggcacc cgccttcact gagcaagcgg ctcggccgag
     4561 tccgacgtgg ctgcgtcagc gcgtcgatca tgcccgcccg gtcgaaccga agccaccgcc
     4621 gccgcgcgca ctgtcctcga aagactcgac gacgtggaag cgcgcctgca cgacgggcac
     4681 gatgacgagt tgcgcgatcc gctcgagcgg ctgcacggta tagggcgacg ttccgcgatt
     4741 ccagcagctc accatgagcg gcccctggta atcggaatcg atgaggccga ccaggttgcc
     4801 gagaacgatg ccgtgcttgt ggccgagtcc ggaacgcggc aggatcatcg ccgccagcga
     4861 aggatctgcg atgtggatgg ccaggccggt ggcgatcagc cgggccgtgc cgggggccag
     4921 cgtcatcggc gcgtcgatgc aggcgcgcag gtcgagcccg gcgctgccgg cggtcgcgta
     4981 cgacggaagc tggtcggcga cgcgcgggtc gagcacgcgc acgtcgatgt cggcgacggg
     5041 acccggcgtc acccgaggct cctggcagcg ctgccgagcc gcgtcgcgat ctcggccacc
     5101 aggcgacgcg ccagcgccag cttgccggcc cgcggccacg cctgctcgcc tcccgcatcg
     5161 acgagggtga tggcgttgtc gtcctcgccg aacgtgtcgg cgccgatgtt ggcgacgatc
     5221 agcggcacct tctttcgctt catcttgtcg cgcgcgtggg ccagcaggtt gtcgctctcg
     5281 gccgcgaagc cgacgcagta aggcggcttc gcgagcgccg cgaccgccgc gaggatgtcg
     5341 gggttttcga ccagcgccaa cgcgggaacc cgtcggtcgg tggtcttctt gatcttcgcc
     5401 gcggacactt cggcagcccg ccagtcggcg accgccgcgg tggccacgaa gacgtc
//
LOCUS       DASRWS010000315         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_128594, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000315 DASRWS010000000
VERSION     DASRWS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128594"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFF72_05885"
     CDS             <1..456
                     /locus_tag="VFF72_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV92727.1"
                     /translation="IGYGRPGAGQAEIEAAARAARIHDFIVSTPKGYQTPVGERGLKL
                     SGGEKQRVAIARTLLKDPPIVIFDEATSALDSANERAIQAELQSAARNRTTLVIAHRL
                     STVVDAHQILVMEQGRIVERGTHAELVARDGRYAEMWRLQQSGAEEAID"
     gene            709..1617
                     /locus_tag="VFF72_05890"
     CDS             709..1617
                     /locus_tag="VFF72_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV92728.1"
                     /translation="MKKQILASLALAAGALAFAGAAHAEDTLKKIKDSGSITEGVRDS
                     SGVLSFTLGDGKYAGFHYEVCAHIIDDIKKHLGMSKIDVKYQPVTSQNRIPLVENGTV
                     DIECGSTTNNAARAKQVAFAPTLYVEEVRIAVKANSGINKIADLNGKTIATTTGTTSV
                     QLLRKNKRAAGMDFKELNGKDHSDSFLLLESGRADAFVMDGQILAGLIAKSKNPADFK
                     IVGEPLSVEPIAIMFRKDDPSFKKLVDDSVKAMIKDGEVAKLYDKWFMKPIPPNNTSL
                     NLPASEATRNAWAHPNDKPTEDYAEK"
     gene            1765..2469
                     /locus_tag="VFF72_05895"
     CDS             1765..2469
                     /locus_tag="VFF72_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV92729.1"
                     /translation="MLDFSFFLHDTGGDETYLHWLMTAWGWTLSVAALSLIVALVAGS
                     VIGIMRTTPSRALVFIGNAWTEFFRNIPLLVQIFVWYYVVPSIFLSLRAVPSYVLVVL
                     ALGLFTSARIAEQVRAGIQSLPKGQRYAGLAMGLTLAQTYRYVLLPMAYRIIVPPLTS
                     ESMNIVKNTSVAFAVSIPELTQFALQAQEETSRGTEIYIAVTFLYFISAFAVNRIARV
                     IERRVQVPGMMIGGKS"
     gene            2469..3134
                     /locus_tag="VFF72_05900"
     CDS             2469..3134
                     /locus_tag="VFF72_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV92730.1"
                     /translation="MQLDFTFFSWKLIQSFILKGLVFSVELTVVAMLGGIAIGTLLAL
                     MRLSGRPWLTRPAAFYVDTLRSIPLVMVILWFFLLIPMLIGRPLGTDWSAIITFTVFE
                     AAYYSEIMRAGIQSVPRGQVGAGYAMGMSYAQCMRLVVLPQAFRNMLPVLLTQTIILF
                     QDTSLVYAIGAYDLLKGFEVAGKNYNRPIEAYLIAAVVYFVICFSLSMLVRQLQRKIA
                     IIR"
     gene            3150..>3755
                     /locus_tag="VFF72_05905"
     CDS             3150..>3755
                     /locus_tag="VFF72_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZV92731.1"
                     /translation="MIDIKNVSKWYGSFQVLTDCSTKIEKGEVVVVCGPSGSGKSTLI
                     KTVNALEPFQKGDIVVDGISLADPKTNLPKLRSRVGMVFQHFELFPHLTVTQNLTLAQ
                     IKVLNRSQDDAMTRGLKMLDRVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMVLLAKEGMTMMVVTHEMGFARKV"
BASE COUNT          694 a         1254 c         1173 g          634 t
ORIGIN      
        1 atcggctacg gccgccccgg cgcgggccag gccgagatcg aggcggcggc gcgcgccgcg
       61 cgcatccacg acttcatcgt ctccacgccg aagggctacc agacgccggt gggcgagcgc
      121 gggctgaagc tctcgggcgg cgaaaagcag cgcgtggcaa tcgcgcgcac cctgctcaag
      181 gacccgccga tcgtcatctt cgacgaggcg acctcggcgc tcgattcggc caacgagcgc
      241 gcgatccagg ccgagctgca gagcgcggcg agaaaccgca cgacactggt catcgcgcat
      301 cgtctgtcga cggtggtcga cgcgcaccag atcctcgtca tggagcaggg acgcatcgtc
      361 gaacgcggca cgcacgccga gctcgtcgcc agggacggcc gatacgccga gatgtggcgc
      421 ctgcagcaaa gcggcgcaga ggaagcgatc gactgagccg tcgccgcagg gaggatttcc
      481 ggaaagcact cgcgcgatcc acctgacgag gcgacagtga agaccggccc ggccagttcg
      541 cgaggcgccg cgctcatgga cgatcgcctc cggcaaaacc cttgcttccc gggcaaacac
      601 gcgcaggccg gtcagccggg ttgcctagaa ttccgttctt ccagagccgc cgggccgtcg
      661 cgtcagcgcc gcccggcaac gaccctgaaa ccttttcgag gaaagcccat gaagaagcag
      721 atcctggcca gcctggcact ggccgccggt gcactggcat tcgccggagc cgcgcacgcc
      781 gaagacacgc tgaagaagat caaggacagc gggtcgatca ccgaaggcgt gcgcgattcg
      841 tcgggcgtgt tgtcgttcac cctgggcgac ggcaagtacg ccggctttca ctacgaggtc
      901 tgcgcccaca tcatcgacga catcaagaag cacctcggca tgtcgaagat cgatgtcaag
      961 taccagccgg tgacttcgca gaaccgcatc ccgctggtcg agaacggcac cgtcgacatc
     1021 gaatgcggct cgacgaccaa caacgccgcc cgcgcgaagc aggtcgcctt cgcgccgacg
     1081 ctctacgtcg aggaggtgcg gatcgccgtc aaggcgaact ccggcatcaa caagatcgcc
     1141 gacctgaacg gcaagacgat cgccacgacg acgggcacca cctcggtcca gctgctgcgc
     1201 aagaacaagc gcgccgccgg aatggacttc aaggagctca acggcaagga ccattccgac
     1261 agcttcctgc tcctcgagtc cggccgcgcc gacgcgttcg tgatggacgg ccagatcctc
     1321 gccggcctga tcgcgaaatc gaagaatccg gccgatttca agatcgtcgg cgagccgctc
     1381 tcggtcgagc cgatcgccat catgttcagg aaggacgacc cgtccttcaa gaagctcgtc
     1441 gacgacagcg tcaaggcgat gatcaaggac ggcgaggtcg ccaagctcta cgacaagtgg
     1501 ttcatgaagc cgatcccgcc gaacaacacc agcctcaacc tgcccgcgtc ggaggcgacc
     1561 cgcaacgcct gggcgcatcc caatgacaag ccgaccgagg attacgccga gaagtgatcg
     1621 gctgacaggc tgaggtaagc cctagcgccc ggcggcgcgg actttgcata cattgctcga
     1681 gagcgcggcg cggagacctt cgcgtcgcgc tcttcgtcat tccggtcgcg tcaagacggc
     1741 cagccacggg agaacggcgc tcgcatgctc gacttcagct tcttcctgca cgacaccggc
     1801 ggcgacgaga cctatctcca ctggttgatg acggcctggg gctggacgct ctcggtcgcg
     1861 gcgctctcgc tcatcgtcgc gctggtcgcc ggctcggtga tcggcatcat gcgcacgacg
     1921 ccgagccggg ccctggtctt catcggcaat gcctggaccg agttcttccg caacatcccg
     1981 ctgctggtgc agatcttcgt ctggtactac gtcgtcccgt cgatctttct ctcgctgcgg
     2041 gctgtgccca gctacgttct cgtcgtcctg gcgctgggcc tgttcacttc ggcgcggatc
     2101 gccgaacagg tccgtgccgg catccagtcg ctgccgaagg gccagcgcta cgcgggcctg
     2161 gcaatggggc tgacgctggc gcagacctac cgctacgtgc tgctgccgat ggcctaccgg
     2221 atcatcgtgc cgccgctcac cagcgaatcg atgaacatcg tcaagaacac ctcggtggcg
     2281 tttgcggtca gcattcccga gctcacccag ttcgcgctgc aggcgcagga agagacctcg
     2341 cgcggcaccg agatctacat cgcggtgacc ttcctctact tcatctcggc attcgcggtc
     2401 aaccggatcg cccgggtcat cgagcggcgc gtccaggtac cggggatgat gatcggaggc
     2461 aagtcgtgat gcagctcgac ttcaccttct tcagctggaa gctgatccag agcttcatcc
     2521 tcaaggggct ggtcttctcg gtcgagctga ccgtcgtcgc catgctcggc ggcattgcca
     2581 tcggcacgct gctggctctg atgcgcctgt cgggcaggcc ctggctgacg cgccccgccg
     2641 cgttctatgt cgacaccctg cgctcgatcc cgctggtgat ggtgatcctg tggttcttcc
     2701 tgctgattcc gatgctgatc ggcaggccgc tgggcaccga ctggtcggcg atcatcacct
     2761 tcaccgtctt cgaggcggcc tactattcgg agatcatgcg cgccggcatc cagtcggtac
     2821 cgcgcggcca ggtcggcgcc ggctacgcga tgggaatgag ctacgcgcag tgcatgaggc
     2881 tggtcgtcct gccgcaggca tttcgcaaca tgcttcccgt cctgctgacg cagacgatca
     2941 tcctgttcca ggacacatcg ctcgtctacg ccatcggcgc ctacgacctg ctcaagggat
     3001 tcgaggtcgc gggaaagaac tacaaccggc cgatcgaggc gtacctgatc gccgcggtcg
     3061 tctacttcgt catctgcttc tcgctgtcga tgctcgtgcg tcaactgcag aggaagatcg
     3121 ccatcatccg ctaacgcgcc aggccgaaca tgatcgacat caagaacgtc tcgaagtggt
     3181 acggcagctt ccaggtgctg accgactgca gcaccaagat cgagaagggc gaggtcgtcg
     3241 tcgtctgcgg gccctcgggc tcgggcaagt cgaccctgat caagactgtc aatgcgctcg
     3301 agccattcca gaaaggcgac atcgtcgtcg acggcatctc gctcgccgac ccgaagacga
     3361 acctgccgaa gctgcggtcg agggtcggca tggtcttcca gcacttcgag ctgtttccgc
     3421 atctcaccgt cacgcagaac ctgacgctgg cgcagatcaa ggtgctgaat cgctcgcagg
     3481 acgacgcgat gacgcgcggc ctgaagatgc tcgaccgggt cggcctgatg gcgcacaagg
     3541 acaagtttcc gggccagctc tcgggcggcc agcagcagcg cgtcgccatc gcccgcgcgc
     3601 tcagcatgga tccgatcgtc atgctcttcg acgagccgac ttcggcgctc gatcccgaga
     3661 tggtgggcga ggtgctcgac gtcatggtgc tgctcgccaa ggaaggcatg acgatgatgg
     3721 tcgtcaccca cgagatggga ttcgcgcgca aggtc
//
LOCUS       DASRWS010000316         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2148220, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000316 DASRWS010000000
VERSION     DASRWS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148220"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFF72_05910"
     CDS             complement(<1..584)
                     /locus_tag="VFF72_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZV92732.1"
                     /translation="MNDATAVALSAGFDLKRLPETFYDDPFPTYRALREHEPVKRLAG
                     GGIFLTRHADIVAVYKDVATFSSDKHEEFFPKYGATPLFEHHTTSLVFNDPPLHARVR
                     RLIAGALTPRHVAAMEGRLVELVDGLLDAMAARGGGAIDLIEAFCGAIPVEVIGNLLG
                     VPRGERGAMRRWSLAILGALEPTLTAEQHALGNAA"
     gene            complement(581..1666)
                     /locus_tag="VFF72_05915"
     CDS             complement(581..1666)
                     /locus_tag="VFF72_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZV92733.1"
                     /translation="MKPQTARTAAVIGLIAACLWLLHGLLAPIVWAALIAMATWPLHD
                     RLRAWFGRSREGLSAVLLTGAVVLFLLVPLGYLLYRGAREMPHLLRFWATTEAGGLAA
                     PTWLASLPGIGTWATLRWNETLAVPGALGDYAHSFLGGVDLAKGRSLVGVFAHHAMGL
                     FFCVVVLFALYLHGDELAGQIDAALVRHVGPAGRRTRLLALRSIRGAVNGLVLVGCGV
                     AVFMSFLYLVAGVPHPFAWGLATGLLGMVPFGAGIVIIGVVLYFLANAAMPAAIAVGA
                     IGAIAIFIADHFVRPHFMSGASKLPLVLALLGIVGGLETFGLLGLFLGPTLLAVMLAV
                     WRELSAPHEDASAAGDAAAAPPSRAGA"
     gene            1804..2709
                     /locus_tag="VFF72_05920"
     CDS             1804..2709
                     /locus_tag="VFF72_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZV92734.1"
                     /translation="MSHIIWKGAISFGLVHVPVALYPASQEIGIDFDWLDKRTMDPVG
                     YKRINKRTGKEIDSSDIVKGIKQADGDYVVLSEDEIKAAYPKSTQTIAIEAFVKASEI
                     SFVYLERPYYTAPIGKGDRVYALLREAMRESGVIGIARVVLHTKEYLAALIPDGAALV
                     LNTIRWASEVRPVADIDLPAAGKSAAKLKEGELKMARQLIGDMTTAWKPDAFADEFTS
                     AIHKLAARRVEAGKTEKVTELEDEPATEAAGNVVDLTALLRKSLASRKPAKKSAAGSR
                     SSATKTARKTAGKTAKGPTPRRRAA"
     gene            complement(2724..>2948)
                     /locus_tag="VFF72_05925"
     CDS             complement(2724..>2948)
                     /locus_tag="VFF72_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92735.1"
                     /translation="MQSPERTMNQGAPRQFAEHERSAPERTTPMREAPQGRIGRQAER
                     APMHEAAHQGGGEPHHGGNRGGERGGERER"
BASE COUNT          518 a         1017 c         1051 g          362 t
ORIGIN      
        1 gcggcgttgc cgagcgcatg ctgctcggcg gtgagcgtcg gttcgagcgc tccgaggatc
       61 gccagcgacc atcgacgcat cgccccgcgc tcgccgcgcg gcacgcccag caggttgccg
      121 atcacctcga ccgggatcgc gccgcagaag gcctcgatga gatcgatcgc gccgccgcca
      181 cgcgcagcca tcgcgtcgag caggccgtcg acgagctcga cgaggcgccc ttccatcgcc
      241 gccacgtgcc gcggcgtcag cgctccggcg atcagtcgcc tgacccgcgc atgcagcggc
      301 ggatcgttga agacgaggct cgtcgtgtga tgctcgaaca gcggcgtcgc gccgtacttg
      361 ggaaagaact cctcgtgctt gtcggagctg aacgtggcga cgtccttgta cacggcgacg
      421 atgtccgcgt gccgggtcag gaagatgccg ccgccggcca gccgcttgac gggctcgtgc
      481 tcgcgcagag cccggtaggt gggaaacgga tcgtcgtaga aggtttcggg cagccgcttc
      541 agatcgaagc cggccgacag cgcaacggca gtggcgtcgt tcacgccccg gcccgcgacg
      601 gcggcgccgc cgcggcgtcg ccagcggccg aagcgtcttc gtgcggcgcc gacagctcgc
      661 gccagacggc gagcatcacg gcaagaaggg tcgggccgag gaagagcccg agcaggccga
      721 aggtctccag gccaccgacg atcccgagca gcgccagcac cagcggcagc ttcgacgcgc
      781 cggacatgaa gtgcggacgc acgaagtgat cggcgatgaa gatggcgatc gctccgatcg
      841 cgccgacggc gatggccgcc ggcatcgccg cgttggcgag gaaatagagg acgacgccga
      901 tgatgacgat ccccgccccg aacgggacca tgccgagcag tcccgtcgcg agcccccagg
      961 cgaagggatg cggcacgccc gcgacgaggt agagaaagct catgaacacg gcgacgccgc
     1021 agccgacgag caccagtccg ttgacggcgc cgcgaatcga acgcagcgcc agcagccgcg
     1081 tccgccggcc ggccggcccg acgtgccgaa ccagcgccgc gtcgatctgc ccagcgagct
     1141 cgtcgccgtg gagatagagc gcgaagagga cgacgacgca gaagaacagt cccatcgcgt
     1201 ggtgcgcgaa gacgccgacg agggagcggc ccttggccag gtcgacgccg ccgaggaagg
     1261 agtgggcgta gtcgccgagc gcacccggca cggcgagggt ctcgttccag cgcaaggtgg
     1321 cccaggtgcc gattccgggc agcgacgcga gccacgtcgg cgccgcgagg ccgcccgcct
     1381 cggtcgtcgc ccagaagcgc agcaggtgcg gcatctcgcg cgcgccacgg tagagaaggt
     1441 agccgagcgg cacgagcagg aagaggacga cggcgccggt cagcagcacc gcgctcaggc
     1501 cctcgcggct gcgcccgaac caggcgcgca gccggtcgtg caggggccag gtcgccatcg
     1561 cgatcagcgc cgcccacacg atcggtgcga gcaggccgtg cagcagccac aggcaggcag
     1621 cgatcaggcc gatcacggcg gcggtgcggg cggtctgcgg cttcacttcg aggtctcccg
     1681 ggggcgaagc gtacgcgatg gcgaaaacgc tgtaggcagc cgcccacagg cgcatcaggc
     1741 gagcggcaat ggtccgtccg cgcgaagcgg gtcaccatcg acgctttctc cccggacttc
     1801 gtcatgtcgc acatcatctg gaaaggcgcc atcagcttcg gcctcgtcca tgttccggtg
     1861 gcgctctacc cggcgtcgca ggagatcggc atcgacttcg actggctcga caagcggacc
     1921 atggacccgg tcggctacaa gcggatcaac aagcgcaccg gcaaggaaat cgacagcagc
     1981 gacatcgtca agggcatcaa gcaggccgat ggcgactacg tcgtgctgag cgaggacgag
     2041 atcaaggcgg cgtacccgaa gtcgacgcag acgatcgcca tcgaggcgtt cgtcaaggcg
     2101 agcgagatct ccttcgtcta cctggagcgc ccctactaca ccgcgccgat cggcaagggc
     2161 gaccgcgtct acgcgctgct gcgcgaggcg atgcgcgagt ccggcgtcat cggcatcgcc
     2221 cgagtcgtgc tgcacaccaa ggaatacctg gcggcgctga ttcccgacgg cgccgccctc
     2281 gtcctcaaca cgatccgctg ggcgagcgaa gtgcggcccg tggccgacat cgacttgccg
     2341 gcggccggca agtcggcggc gaagctgaag gagggcgagc tgaagatggc gcgccagctg
     2401 atcggcgaca tgacgacagc gtggaagccg gacgccttcg ccgacgaatt cacctcggcg
     2461 atccacaagc tcgccgcccg gcgcgtcgag gccggcaaga cggagaaggt gacggagctc
     2521 gaggacgagc cggcgaccga ggcggccggc aacgtcgtcg acctgacggc gttgcttcga
     2581 aaaagcctgg cttcgcgaaa gccggccaag aaatctgccg ccggctcgcg ctcgtcggcg
     2641 acgaagacgg ccaggaagac ggctggaaag acggcaaaag ggccgacccc gaggcggcgc
     2701 gccgcctgag ggtccggcgc cgctcagcgt tcgcgctcgc cgccgcgctc gccgccgcga
     2761 ttgccgccgt gatgcggctc gccgccgccc tggtgcgcgg cctcgtgcat cggtgcgcgc
     2821 tcggcctgcc ggccgatgcg gccctggggc gcctctcgca tcggcgtcgt ccgctcgggc
     2881 gccgaccgct cgtgctcggc gaactggcgg ggcgctccct gattcatcgt tcgctcaggc
     2941 gattgcat
//
LOCUS       DASRWS010000317         7625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3874920, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000317 DASRWS010000000
VERSION     DASRWS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3874920"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VFF72_05930"
     CDS             <1..1355
                     /locus_tag="VFF72_05930"
                     /inference="COORDINATES: protein motif:HMM:NF018454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HZV92736.1"
                     /translation="GLRPSESTRSLPLGKANVELTNRVRRLYFEEYIKVWDRYLADVR
                     IVKLQGLEQSLQVARALSGIDSPLAAFMRGVSRETTLVQPATTAANNANAPAQLAQAD
                     QKAAQAKREIAAVLGKLNAPGSGAPQGPPLEQMVDDHFANIHRLTAGQPPPMDDILKL
                     FNDVYVQLAAVDAAQKSKSAPPPAGGGERIAAAAGQLPEPARSMLEKLAGAAATSGRV
                     VERQGLTSDLKPISEFCNRAITGRYPFTASSKADVLPEDFAQLFGPGGMLDDFYNRKL
                     ASLVDTGTNPWRFKPVGDGSKPVNAAALVDFQRAARIKEVFFRSGGKTPSFKVDVRAL
                     DMDESLKDITLEIDGTPIKFVAGNTTPVTIQWPSARVASQIRLSSSSGITPLTFDGPW
                     ALFRLFDRFDVQPTAQPERFIVALNLDGKRARLEVTASSVFNPFRLREIQQFRCPGSL
                     "
     gene            1378..2547
                     /gene="tagF"
                     /locus_tag="VFF72_05935"
     CDS             1378..2547
                     /gene="tagF"
                     /locus_tag="VFF72_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HZV92737.1"
                     /translation="MNLASDIAATPGWYGKLPTLGDFASRRLEADFIEPWDLWLGEGL
                     QAQRDALGEGWLDAYLQSPVWRFVLQPGALASFEPSLAIVGILMPSVDRVGRYFPLTI
                     AASMRFLPGEARQFDALLEWLRRLEDTGLAALQQDWTIGRLEEALVEVGSPIQMLQGR
                     EDRLAPNRRSIEAALSSGGFVDLQQPLDRDDIAMLLVDVARPVGFASVPPGDAHRRPG
                     ERGVALWLADTGSPPRLLLSRGLPAQADFVAMFSGAAESVSSAPPTGRHDETAWPAAA
                     TSVVAGAPNVDPDGSDVLGLFASPVPRGSEEETTRPVPLGAWPDPDATAPGELTSDSV
                     ALPSDKSQNEDILALFDATPQTHAPESELETRPGSPDILDLFGTSKLKPDFDKTR"
     gene            2553..4460
                     /locus_tag="VFF72_05940"
     CDS             2553..4460
                     /locus_tag="VFF72_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZV92738.1"
                     /translation="MSNDDEATRIIAGTPTSRAPLPPSAPPVAISSDAEMPPEEGDAT
                     IIRPKPKIPPPAAEGGNMLPVGFYLGEFEITQVLGEGGFGIVYLAQDHSLGRRVALKE
                     YMPSSLAQRVGGTQVSVKSERHRDTFEAGLKSFVNEARLLAQFDHPSLVKVYRFWEAN
                     GTAYMVMPFYEGITLKDELKAMGAPPDEAWLRDLLEPLTEALAVIHAEQCFHRDIAPD
                     NVILLKGSNLPLLLDFGAARRVIGDMTQALTVILKPGYAPVEQYAEVPGMKQGPWTDV
                     YALAAVVYYAITGKTPPTSVGRLMNDNYVPMTQAGAGRYGNEFLAAMDRALVVKPEAR
                     TQSIDELRRDLGMEPVVADAAKTQRRSGPSTVAAALPAAAAVARKSRVGVFAGIGVVV
                     VLAIAGGVYGWIGSRPATVATAAGPSTATNGGPTTSATVASPAAVTGASPASQVTAAA
                     GPEHAAPVAPAPAGPFDPAREFERVAAAQSPDYKVEAAADKPQLRIDKDKLTFKVSSD
                     KPGHLYVLMHGTDGSIIQLFPNAAAKNNQIRAGSTLSLPAAKGIWDISVAGPPGTDHY
                     LAIVSKWPRDFSALGLKMRDGFEQTTFGAAKAAAEAQAGASSVFMGRPICEQPCTDDY
                     GAAVFNADEIN"
     gene            4503..6290
                     /locus_tag="VFF72_05945"
     CDS             4503..6290
                     /locus_tag="VFF72_05945"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HZV92739.1"
                     /translation="MDDDDWTPDFGTLEETAPEWASKRPVRIAVLGDFSAGAAAGRLE
                     TGDDLARRKMIPVEFDNLEDTLARLEVKLALPIGQGGDGVEVEFSELDSFHPDSLYRE
                     LPMFKSLADLRKRLNNTATFAKAAADVQAMREGGKRRASRSGARRSRSGAPAADAKLS
                     DFARLVGSAAEVRVDAPVDALLKRIVGPFVTATADPKRDAMVTIVDNALSDAMRTVLH
                     QPEFQNLEALWRGMDMLLRRVETGPSLQVLLVDMSAEEFAADLSSASDLSETGLYSML
                     VDKPSQDKAGGVSLILGLYQFEATPPHAELLGRMAKIARLAGAPFITSITADAFMDRR
                     NAPHPLMSSAMEALRELPEASHLALLAPRFMLRHPYGKKSDPVSAFAFEEFSAEEGLR
                     GMLWGHPSLLAASLLAAPVGNTLSIGDLPFHYVVDADGDQVALPTTERLVNLAAAEML
                     RRVGIDALMAHKGQPELRVAGLDTVNGTQIVLAGLPKPAARMSFTTAVQGKLPDAEGG
                     GRSSKGKDADDEPSDSGSDDTSGGDSDDTSSDSSADSSGDSGLDDLLAGLGEDSAAPS
                     EDADPASDEAPAADAEMDPDLAELLKSLE"
     gene            complement(6306..6548)
                     /locus_tag="VFF72_05950"
     CDS             complement(6306..6548)
                     /locus_tag="VFF72_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZV92740.1"
                     /translation="MKADPRQATVTPPRRTLRCPACGGESVYALSNPARPFCSARCRL
                     TDLGAWASEGYRVASSEPRDDAEESVSGDPDPASAR"
     gene            complement(6545..7300)
                     /gene="zapD"
                     /locus_tag="VFF72_05955"
     CDS             complement(6545..7300)
                     /gene="zapD"
                     /locus_tag="VFF72_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HZV92741.1"
                     /translation="MVLYEYPFNESIRTMLRLEHLFDRLGQLIERNARVDHHHALATI
                     FEIMDVGSRADLKSDLLKELEKHRQQLVGFRGNPSITQSVLDDVIGRIDGAHARLNQV
                     AGKAGATLATNEWLMSIRSRINIPGGTCEFDLPAYYAWQQHDATRRRTDLLQWVSTLM
                     PLAEALQVLLGLLRDSGTPHKVVAPGGQYSQSLAAGRVFQLIRVRLDDGAELVPEISG
                     HRLMVQVRLMRQESDGRLRPNAADTTFELTLCA"
     gene            complement(7424..>7625)
                     /locus_tag="VFF72_05960"
     CDS             complement(7424..>7625)
                     /locus_tag="VFF72_05960"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZV92742.1"
                     /translation="CAEATQVARVVQRSGWTTEAVQRTIAQQASRAQRRAIADAVIVN
                     DDLSLAQLDAEVDALSAAWVAP"
BASE COUNT         1291 a         2544 c         2553 g         1237 t
ORIGIN      
        1 tcggcctgcg tcccagcgag tcgacgcggt cgctgccgct cggcaaggcc aacgtcgagc
       61 tgacaaatcg tgtgcgtcgc ctctacttcg aggagtacat caaggtctgg gaccgctatc
      121 tggccgatgt gcgcatcgtc aagctccagg ggctcgagca aagcctgcag gtggcgcgtg
      181 cgctgtcggg aatcgactcg ccgctggccg cgttcatgcg aggcgtctct cgcgagacga
      241 ccctggtgca gcccgcgacg acagcggcca acaatgcgaa cgcgccggcg caactggccc
      301 aggccgacca gaaggcggcg caggccaagc gggagattgc cgccgtcctc ggcaagctca
      361 acgcgccggg aagcggcgcg ccgcagggac cgccgcttga gcagatggtc gacgaccact
      421 tcgcgaacat tcatcgcctc acggccggac agccgccgcc gatggacgac atcctcaagc
      481 tgttcaatga tgtgtatgtg cagctcgcgg cggtcgatgc ggcgcagaag agcaaatcgg
      541 caccgcctcc cgcgggtggc ggcgagcgca tcgctgctgc ggccggccag ttgcccgagc
      601 ccgccagatc gatgctcgag aagctcgccg gcgcggcagc gacatccggc cgcgttgtcg
      661 agcgtcaggg cctgaccagc gatctcaagc cgatcagcga gttctgcaac cgcgcgatca
      721 ccggccgcta tccctttacc gcaagctcca aggcggatgt cctgcccgaa gacttcgcgc
      781 aactgttcgg ccccggcggc atgctcgacg atttctacaa tcgcaagctg gccagcctgg
      841 tcgataccgg caccaacccc tggcgtttca agcccgtcgg agatggatcg aagcccgtga
      901 acgcggcggc gctcgtcgac ttccagcgcg cggcgcggat caaggaagtc ttctttcgca
      961 gtggcggcaa gaccccttcc ttcaaggtcg atgttcgagc cctcgacatg gacgagagcc
     1021 tcaaggacat cacgctggag atcgacggca cgccgatcaa gttcgtggcg ggcaacacga
     1081 ctccggtgac gatccagtgg ccgagcgccc gggttgcatc gcagatcagg ctctccagct
     1141 catccgggat cacgccgctg accttcgacg gaccgtgggc gctgttccgg ctcttcgatc
     1201 gtttcgatgt gcaaccgacg gcgcaaccgg aacgcttcat cgtggcactc aaccttgacg
     1261 gcaagcgggc gcggctcgag gtgacggcga gcagcgtctt caatccgttt cgcctgcgtg
     1321 aaatccagca attccggtgt ccgggttcgc tatgagtccc gggcccgcga cgcatcgatg
     1381 aatctcgcca gcgatatcgc cgcgacaccg ggttggtacg gcaagctgcc cacgctgggc
     1441 gacttcgcct cgcgccgact cgaagcggac ttcatcgagc cgtgggatct ctggctcggc
     1501 gagggtctgc aggcgcagcg cgatgcgttg ggcgaaggtt ggctcgacgc ttatctgcaa
     1561 agccccgtct ggcgtttcgt tctgcagccc ggcgcgctgg cgtccttcga accgagcctg
     1621 gcgattgtgg gcatcctgat gccgtcggtc gatcgcgtcg gccgttattt tccgctgacc
     1681 attgccgcgt cgatgcggtt cttgccgggc gaggcgcggc aattcgacgc cttgctcgaa
     1741 tggcttcgtc gcctggaaga caccgggctc gccgccctgc agcaggactg gaccatcggc
     1801 cggctcgaag aggcgctggt cgaggttggc tcgccgattc agatgctgca aggccgcgag
     1861 gatcggctgg cacccaatcg ccggtcgatc gaggcggcgt tgtccagcgg cggcttcgtc
     1921 gacctgcagc agcctctgga ccgcgacgat atcgcgatgc ttcttgtcga cgtggcacga
     1981 cctgtcggtt tcgcttccgt gccgccaggc gatgcccatc gccggcccgg cgaacgaggc
     2041 gtagccctgt ggctcgccga caccgggagc ccgcctcgtt tgctgctcag ccggggcctg
     2101 ccggcgcagg ccgatttcgt cgcgatgttc agcggagctg ccgaatcggt ttcatcggcg
     2161 ccgccgactg gccggcacga cgaaacggct tggccggccg cggcgacatc ggtcgtcgcc
     2221 ggagcgccca atgtcgatcc cgacggaagc gacgtgctgg gcctgttcgc gtcgcccgtc
     2281 ccccggggca gcgaagagga aacgacgcgg ccggttccgc tcggtgcatg gcccgacccc
     2341 gatgccacgg caccgggcga actgacgagc gactctgtgg ctctccccag cgacaagtcg
     2401 cagaacgagg acatcctcgc cctgttcgat gcgacgccgc agacccatgc gccagagtcc
     2461 gaactggaga cccggccggg atcgccggac attctcgatc tgttcggcac atcgaaattg
     2521 aagcccgact tcgacaagac acgctgagtc tcatgagcaa cgacgacgaa gcgactcgca
     2581 tcattgccgg cacgccgacg agccgcgccc cgctgcctcc gtcggccccg ccggtcgcga
     2641 tatcgagcga cgccgagatg ccacctgaag agggcgacgc gacgatcatc cgaccaaagc
     2701 cgaagatccc accgcccgcg gcggaaggcg gcaacatgct gcccgtcggg ttttatctcg
     2761 gcgagttcga gatcacgcaa gtgctcggcg agggcgggtt cggaatcgtc tatctggccc
     2821 aggaccattc gcttggacgc cgggtggcgc tgaaggaata catgccctcg tcgctggccc
     2881 agcgcgtcgg cggcacgcag gtgtcggtga agtccgaacg ccatcgcgac accttcgagg
     2941 cgggactgaa gagcttcgtc aacgaagcgc gactgctcgc ccagttcgac catccgtcgc
     3001 tggtcaaggt ctatcggttc tgggaggcca acggcacggc ctacatggtg atgccgttct
     3061 acgaaggcat caccctcaag gatgaattga aggccatggg cgcgccgccc gacgaggcat
     3121 ggctgcgcga ccttctcgag cccttgacgg aagcgctcgc ggtgatccac gccgagcagt
     3181 gctttcaccg cgacatcgcc cccgacaacg tgatcctgct caagggcagc aacctgcccc
     3241 tgctgctcga cttcggcgcc gcccggcgcg tcatcggcga catgacccag gctctgacgg
     3301 tgatcctcaa gccgggctat gcgccggtcg agcaatacgc cgaggtgcct ggcatgaagc
     3361 aggggccgtg gacggacgtc tacgcgcttg ccgccgtcgt ctactacgcc atcacgggca
     3421 agacgccgcc gacatcggtc ggccggctca tgaacgacaa ctacgtaccg atgacgcagg
     3481 ccggcgccgg gcggtacggc aacgaattcc tggcggcgat ggaccgggcg ctggtcgtca
     3541 agcccgaagc gcgcacgcag tcgatcgacg agctgcggcg cgacctcggc atggagccgg
     3601 tcgtcgccga cgcagccaag acacagcgtc gcagcggccc gtcgacggta gccgcggcgt
     3661 tgcctgccgc ggccgccgtc gcgcgaaaga gcagggtcgg cgtgttcgcg ggcatcgggg
     3721 tcgtcgtcgt gctggccatc gccggcggtg tctatggctg gatcggttcc aggccggcca
     3781 ccgtggcgac ggcggccggc ccttcgactg caacgaacgg cggcccgacc acctcggcga
     3841 cggtcgcgtc cccggccgcg gtcaccggcg ccagtcctgc atcgcaggtc accgccgcgg
     3901 cgggcccgga gcacgccgcc cctgtggcgc cggcgccagc gggcccgttc gatccggccc
     3961 gcgagttcga gcgtgtcgcg gcggctcaat cgcccgacta caaggtcgag gccgcggccg
     4021 acaaaccgca gttgcggatc gacaaggaca agctgacctt caaggtgagc agcgacaagc
     4081 cgggtcatct ctacgtgctg atgcacggca ccgacggaag catcatccag ttgtttccga
     4141 acgctgcggc gaagaacaac cagattcgag ccggctcgac gctttctctg ccggccgcca
     4201 agggcatctg ggacatcagc gtcgcggggc cgcctggcac cgatcattac ctggccatcg
     4261 tgtcgaagtg gcctcgcgat ttctcggccc tcggcctgaa gatgcgcgac ggcttcgagc
     4321 aaaccacctt cggtgccgcg aaggcggccg ccgaggcgca ggccggcgca tcgtcggtct
     4381 tcatgggccg gccgatctgc gaacaaccct gcaccgacga ctatggagcc gcggtgttca
     4441 acgccgacga gatcaattga tgccgggggc tgaactcatg cccggctgtt gatggagaaa
     4501 tcatggacga cgacgactgg acacccgatt tcggaacgct cgaagagacc gcgcctgaat
     4561 gggcgagcaa gcgaccggtg cgaatcgccg tgcttggcga cttcagcgcc ggggcggcgg
     4621 caggtcgcct cgaaaccggg gacgatcttg cgcgtcgaaa gatgatcccg gtcgaattcg
     4681 acaacctcga agacacgctg gcccggctcg aagtgaagct cgcgttgccg atcggccagg
     4741 gcggcgatgg cgtcgaggtc gaattcagcg aactggacag cttccatccc gactcgctct
     4801 accgcgaatt gccgatgttc aagtcgctcg ccgatctccg caagcggctc aacaacactg
     4861 cgacctttgc caaggccgcg gccgacgtac aggcgatgcg cgagggcggg aagcggcgtg
     4921 cctcgcgcag cggcgctcgt cgctcgcgca gcggcgctcc cgccgccgac gcgaagctct
     4981 cggactttgc ccgtctcgtc ggcagcgccg ccgaggtgcg tgtcgacgcg ccggtcgatg
     5041 cgctgctcaa acgaatcgtc gggccgttcg tgacggcgac ggccgacccc aagcgcgacg
     5101 ccatggtgac gatcgtcgac aacgcgttgt ccgacgcgat gcgcacggtg ctgcaccagc
     5161 cggagttcca gaacctcgag gcactctggc gcggcatgga catgctcctt cgacgcgtcg
     5221 agaccgggcc gtcgctgcag gtgctgctgg tcgacatgtc ggccgaggaa ttcgctgccg
     5281 acctgagcag cgccagcgac ctcagcgaga cagggctcta ttcgatgctt gtcgacaagc
     5341 cgtcgcaaga caaggctggc ggcgtctcgc tcatcctcgg cctctaccag ttcgaggcga
     5401 cgccaccgca cgcggagctg ctcggacgga tggccaagat cgccaggctt gcaggcgctc
     5461 cgttcatcac atcgatcacc gccgacgcct tcatggatcg aaggaatgcg cctcatccgc
     5521 tcatgtcgag tgcgatggaa gcgctgcgcg aattgcccga ggcgtcgcac ctcgcgctgt
     5581 tggcgccacg cttcatgctg cgtcatcctt atggcaagaa gagcgatccg gtcagcgcgt
     5641 tcgcgttcga ggaattcagc gccgaggaag ggctgcgcgg catgctctgg gggcacccgt
     5701 ccctgctcgc ggcgtccctt ctcgccgcgc ctgtgggcaa cacgctatcg atcggcgacc
     5761 tgccgtttca ctatgtcgtc gatgccgacg gcgatcaggt tgccctgccg acaacggagc
     5821 gcctggtcaa cctggcagct gcagagatgc tgcgccgcgt cggcatcgac gcgctgatgg
     5881 cgcacaaggg tcagcccgag cttcgtgtcg ccgggctcga taccgtcaat ggcacgcaga
     5941 tcgttctcgc cggcttgccg aagccggcgg cccgaatgtc gttcacgacc gcggtgcaag
     6001 gcaagctgcc tgatgccgaa ggcggcggtc ggtcgagcaa ggggaaggat gccgacgacg
     6061 agccgagcga cagcggaagc gacgatacga gtggtggcga cagcgacgac acgtcgagcg
     6121 attcgtcggc ggacagcagc ggtgactcag gcctggatga tcttctggcg ggtctcggcg
     6181 aagacagcgc cgcgccgagc gaagacgccg acccggcatc cgacgaagcg ccggctgccg
     6241 atgccgaaat ggatcccgac cttgcggagc tgctgaagtc gctcgagtga acgcgatcgg
     6301 cgctttcagc gcgctgatgc ggggtcggga tcgccgctga cgctttcctc ggcgtcgtcc
     6361 cggggctcgg acgaggcgac ccggtagcct tcgctggccc atgcgcccag gtccgtcaga
     6421 cggcaacggg cgctgcagaa cggccgagcc ggattcgaga gggcgtagac gctctcgcca
     6481 ccgcacgccg ggcacctcag ggtgcggcga ggcggggtga ccgtggcctg acgcggatcg
     6541 gccttcatgc gcaaagcgtc agctcgaagg tcgtgtcagc ggcgttcggt cgcaggcgcc
     6601 catcggattc ctggcgcatc agccgaacct ggaccatcaa ccgatgtccg ctgatctcgg
     6661 gaacgagctc ggcgccgtcg tcgagtcgga cgcggatgag ttgaaagacg cgtccggcgg
     6721 cgaggctttg cgaatactgg ccgccggggg cgacgacctt gtgcggcgtc ccggagtcgc
     6781 gcagcaggcc gagcaacacc tgcagtgcct cggccagcgg catcaaggtc gacacccact
     6841 gcaggagatc ggtccgccgg cgtgtcgcgt catgctgctg ccacgcgtag tacgcgggca
     6901 ggtcgaactc gcaggtgcct ccgggaatgt tgatgcggct gcggatgctc atcagccact
     6961 cgttcgtcgc cagcgtcgca ccggccttgc cggccacctg gttgagccgg gcgtgggcgc
     7021 cgtcgatgcg accgatgacg tcgtcgagga ccgactgggt gatcgaaggg ttgcctcgga
     7081 agccgaccag ttgctggcgg tgcttttcga gctccttgag aagatcggac ttcagatcgg
     7141 cgcgcgaccc gacgtccatg atctcgaaga tcgtcgccag tgcgtggtga tggtcgaccc
     7201 gagcgtttct ctcgatcagc tggcccaggc gatcaaagag gtgttcgagg cgaagcatcg
     7261 tgcggatgct ttcgttgaac gggtattcgt ataggaccaa ggcgttctct ggttcggctg
     7321 tgaccgcgat tgtttcacac gcgttacgac cttcgcgccc cccaaggcgc atgggcgcgc
     7381 aatctccagc tttgtcctac aagaagcagg ggacccggac cgctcagggc gcgacccacg
     7441 ccgccgacaa cgcgtccact tcggcgtcca gctgcgccag ggacaagtcg tcgttgacga
     7501 tcaccgcgtc ggcgatggcg cgccgctgtg cgcgcgacgc ctgttgcgcg atcgtccgtt
     7561 ggacggcctc ggtcgtccag ccagagcgct gcacgacgcg ggcgacctgc gtggcctcgg
     7621 cacag
//
LOCUS       DASRWS010000318         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4163236, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000318 DASRWS010000000
VERSION     DASRWS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163236"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..777
                     /locus_tag="VFF72_05965"
     CDS             166..777
                     /locus_tag="VFF72_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92743.1"
                     /translation="MIGESRRRCVGVSAFVFLALLMLPVKHVLEASMTAQMLLQIPLL
                     VGVGCLLSRALPARAQAAADDWNDRGITGLVLASVAGAFWMLPRMLDAAVTEPLIDAA
                     KYLSVPLLIGVPFAISWPRMTFIVRGVFLVEFIATLFRLGWLYLISPERLCNVYLLDD
                     QQRLGRYMLLIGAALFLAFAAKLIFGRFDSLVAPQPTSPSATR"
     gene            862..1323
                     /locus_tag="VFF72_05970"
     CDS             862..1323
                     /locus_tag="VFF72_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZV92744.1"
                     /translation="MQLTRFTDYALRVMLFVGRQHGHICTMGEIATYYGISLEHLRKV
                     VHKLAKLGYLRSSRGRGGGLLLGHDPAGIRIGDVILAMEEDLSIVDCVAVDCALAPGC
                     SLKTALDRACAAFIASLNEVSLADLLRDRGMRRQFKNIDIAIASRAAARRS"
     gene            complement(1407..>2528)
                     /locus_tag="VFF72_05975"
     CDS             complement(1407..>2528)
                     /locus_tag="VFF72_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92745.1"
                     /translation="GGVGPGVSPNVALTVAGLKVDVDALPGNLQAKLKQGKVDLDSPA
                     TTLALLKLNSVVGVKGFFDKGGSLSSIGITCALCHSTVDDSFAPGIGKRLDGWPNQEL
                     NTGLIVSLAPDLAPVAEQVLGASDEATIDTVKKVLLSWGPGFYDAELNIDGIGFRPDG
                     KSAAVRIPAAYGHLGEDLHTWTGGFGNVTYWNAYVANLQMHGNGNFNDERFDDPAKYP
                     AAVRAGSFKVRHNPDLVTSKLAPLHFYQLSLQAPKPPAGSFDPAAAKRGEAVFNGQGK
                     CTDCHMPPLYTDAGYNAHTPTEMCIDSFQADRGPTGAIGSTYVTPQLAGLWARSKRGF
                     FHDGRFPTLLDVADHYNACFNLGLSQSQKSDLVEFLKSR"
BASE COUNT          391 a          806 c          829 g          502 t
ORIGIN      
        1 acgactcgag cgagctttcg gtatcaccgt catcccggat ggggctggcg gctatacaca
       61 caacgcggcg atccatctcg tcgatccgcg cggccgactc gttgcgatat tcgacgccga
      121 cgatccgcgg ctcgtcgccc acgccttgct gcagagcctg gatcgatgat cggcgaatcg
      181 cggcgccgct gcgtcggcgt ctcggcattc gtgttcctcg ctttgctgat gttgccggtg
      241 aagcacgtgc tcgaagcgag catgaccgcg cagatgctgc tgcagatccc gttgctcgtc
      301 ggcgtcggtt gcctgttgtc gcgggcgcta ccggcgcgcg cgcaggcggc cgcggacgac
      361 tggaacgatc gcggcatcac cgggctggtg ctcgcgagcg tcgccggcgc gttctggatg
      421 ctgccgcgca tgctcgatgc ggcggtgacc gagccgctga tcgacgcggc caagtatctt
      481 tcggtgccgc ttctgatcgg agtgcctttc gcgatcagct ggcctcgcat gaccttcatc
      541 gtccgaggcg tattcctggt cgaattcatc gcgacgctat ttcgcctggg ctggctctac
      601 ctgatatcgc ccgagcggtt gtgcaacgtc tatctgctcg acgaccagca gcgtctcggc
      661 cggtacatgc tgttgatcgg cgccgcgttg ttcctcgcat ttgccgccaa gctcatcttc
      721 ggtcgcttcg attcgctcgt ggcgccgcag ccgacgtctc catcggcgac gcgctgatgc
      781 gcggtgtata ttcaagctcg cgccgaaagg cgctctcgtt gcggccgttc tcgcgcaacg
      841 cttccaatcg tcgttaccac catgcaactg acccgcttta ccgattacgc gttgcgcgtg
      901 atgcttttcg tcggaaggca gcacggccat atttgcacga tgggcgagat cgcgacgtac
      961 tacggcatct cgctcgagca cctgcgcaag gtggtccaca agctggcgaa gctgggttat
     1021 ctccgttcct cccgcgggcg gggcggcggc ctgttgctcg gtcacgaccc ggccgggata
     1081 cgcatcggcg acgtcatcct cgccatggag gaagatttga gcatcgtcga ttgcgttgcg
     1141 gtggactgcg cgctggcgcc cggatgctct ctgaaaaccg ccctggaccg cgcttgtgcg
     1201 gcgttcatcg cgagcctgaa cgaggtgtcg cttgccgatc tgctgcgcga ccggggcatg
     1261 cgacggcagt tcaagaacat cgacatcgca attgcgagcc gtgctgctgc cagacgctct
     1321 tgagcaacgc ggaaaggatc ggacggtgaa cgctgcgaat gccccaggct gcgcagcgtt
     1381 tccgccatcg acggcctctc tcccgcctag cgactcttga ggaactcgac cagatcgctc
     1441 ttctgacttt gcgacaggcc gaggttgaaa cacgcgttgt agtgatcggc cacatcgaga
     1501 agcgtcggga aacggccgtc gtggaagaag ccgcgcttcg agcgcgccca gaggccggcg
     1561 agttgcggcg tgacgtaggt gctgccgatc gctcccgtcg gtccacggtc ggcctggaag
     1621 gagtcgatgc acatctcggt cggcgtgtgc gcgttgtacc ccgcgtcggt gtacagcggc
     1681 ggcatgtggc agtccgtgca tttgccctgg ccgttgaaga ccgcctcacc ccgtttcgcc
     1741 gccgcgggat cgaagctgcc ggcaggcggc ttcggtgctt gcagcgagag ctggtagaag
     1801 tgaagcggcg ccagcttcga cgtcaccagg tccgggttgt ggcgcacctt gaaagagccg
     1861 gctcgcaccg ccgccgggta cttggccgga tcgtcgaacc gctcgtcgtt gaagttgccg
     1921 ttgccgtgca tctgcaggtt cgcgacatac gcgttccagt aggtgacgtt gccgaagcct
     1981 cccgtccatg tgtggagatc ctcgccgagg tgaccgtacg cagccgggat ccggacggcg
     2041 gccgacttgc cgtccggtcg gaatccgatg ccgtcgatgt tgagttcggc gtcgtagaag
     2101 cccgggcccc agctgaggag caccttcttg accgtgtcga tcgtcgcttc gtcgctggcc
     2161 ccgaggacct gttcagcgac gggcgccaga tcgggcgcga gcgagacgat caagccggtg
     2221 ttcagttcct ggttcggcca tccgtccagc cgcttgccga ttcccggagc gaacgagtcg
     2281 tcgaccgtcg aatggcaaag cgcgcaggtg atgccgatcg agctgagcga gccacccttg
     2341 tcgaagaagc ccttgacgcc gacgaccgaa ttcaacttca ggagcgcgag ggtggtcgca
     2401 ggactgtcga gatcgacctt gccctgcttg agcttcgcct ggaggttccc cgggagggca
     2461 tcgacatcga ccttcaggcc ggcgacggtg agggccacgt tcgggctgac accggggccg
     2521 acacctcc
//
LOCUS       DASRWS010000319         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3011543, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000319 DASRWS010000000
VERSION     DASRWS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3011543"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFF72_05980"
     CDS             <1..332
                     /locus_tag="VFF72_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZV92746.1"
                     /translation="PGFPVMAWPMIEWVLDHVYANRHVANAIRERSVIVFGAMEAALT
                     PLMEAIEREFPGVKVFSLPSVDHPQWGRHIELGVKGDPLRLEAAYATLIEGLRTQGAK
                     LGPELVR"
     gene            506..1918
                     /gene="glnA"
                     /locus_tag="VFF72_05985"
     CDS             506..1918
                     /gene="glnA"
                     /locus_tag="VFF72_05985"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZV92747.1"
                     /translation="MAKTVADVMNMVKESEVKFVDFRFTDTRGKEQHVSVPISAFDED
                     KFNSGHAFDGSSIAGWKGIEASDMLLMPDPNTANIDPFYEEPTLILTCDVLEPGDGKP
                     YERDPRSLAKRAEAYMKASGLGDAAYFGPEPEFFIFDSVRWQVDMSGSFCKVESEEAA
                     WNSAKEYEHGNSGYRPTVKGGYFPTPPVDSMQDMRSEMCLILESLGIPVEVHHHEVAQ
                     AQMEIGTKFSTLVERADWLQLQKYVIQNVAHSYGKSATFMPKPIVGDNGSGMHVHQSV
                     WKGGKNLFAGDGYAGLSEFALFYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKL
                     AYSSRNRSASIRIPYVPNPKGRRIEARFPDPQANPYLAFAALLMAGLDGVENKIHPGE
                     AATKDLYHLPPEEDAKIPTVCSSLDQALDYLDKGRAFLTKGGVFTDGYIDAYIELKMQ
                     EVTRFRMTTHPVEFDMYYSL"
     gene            2092..2628
                     /locus_tag="VFF72_05990"
     CDS             2092..2628
                     /locus_tag="VFF72_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92748.1"
                     /translation="MSMRPSIPCAAAALLAAMLVGPGVARAQASASEGSVTYRCPGND
                     YKNTISAKEAERLGCKKLEGVPITIIQTSRPRQASPAGAGSSNARVDPQAQRARDTDA
                     RRILEQELKSEEDRLAGLKKDFNNGQPERHGNEQNYQKYVDRVADLSAAIARKEGDIA
                     AIKREIQKLPPAPPQPVL"
BASE COUNT          473 a          889 c          838 g          430 t
ORIGIN      
        1 tgccgggctt tccggtgatg gcctggccga tgatcgaatg ggtgctcgat cacgtgtacg
       61 cgaaccggca cgtggccaac gccatccgcg agcgctcggt catcgtcttc ggtgccatgg
      121 aagccgcgct gacgccgctg atggaggcga tcgagcgcga gttcccgggc gtgaaggtgt
      181 tcagcctgcc gagcgtcgac cacccgcaat ggggccggca catcgagctt ggcgtgaagg
      241 gtgatccgct tcggctcgag gcggcgtacg ccaccttgat cgagggcctg cgcacccaag
      301 gcgcgaagct cggccccgag ttggtgcgat gatgcccgga ctggtgcccg cggccggtgc
      361 ggcggcgcgc accgcgatgg cctcgccggg gcacgtccat tggcatgctt tctgctaaat
      421 tttcgtgcgc atggcggggc cgcggtcccc gcgcgagcgc gtcgcagatc ccacacgcaa
      481 gcagtccttt ttcggagatc cctggatggc caagactgtc gccgacgtca tgaacatggt
      541 gaaggagagc gaagtcaagt tcgtcgactt ccgcttcacc gatacccgcg gcaaggagca
      601 acacgtctcg gtgccgatct cggccttcga cgaagacaag ttcaattcgg gccacgcgtt
      661 cgacggttcg tcgatcgctg gctggaaggg catcgaggcg tcggacatgc tcctcatgcc
      721 cgacccgaac accgccaaca tcgacccgtt ctacgaggag ccgacgctca tcctcacgtg
      781 tgacgtcctc gagccgggcg acggcaagcc ctacgagcgc gacccgcgct cgctcgccaa
      841 gcgcgccgag gcgtacatga aggcatcggg gctcggcgac gcggcgtact tcggccccga
      901 gcccgagttc ttcatcttcg acagcgtccg ctggcaggtc gacatgtcgg gctcgttctg
      961 caaggtcgag tcggaggagg ccgcctggaa ttccgccaag gagtacgagc acggcaactc
     1021 cggctaccgg ccgacggtca agggcggtta tttcccgacg cctccggtcg actcgatgca
     1081 ggacatgcgc tccgagatgt gcctgatcct cgagtcgctc ggcatcccgg tcgaagtgca
     1141 tcaccacgaa gtcgcccagg cgcagatgga gatcggtacc aagttctcga ccctggtcga
     1201 gcgcgccgac tggctccagc tgcagaagta cgtgatccag aacgtcgccc attcgtacgg
     1261 caagagcgcg accttcatgc ccaagccgat cgtcggcgac aacggctcgg gcatgcacgt
     1321 gcaccagtcg gtgtggaagg gcggcaagaa cctcttcgcc ggcgacggct atgcgggcct
     1381 gtcggagttc gccctgttct acatcggcgg catcatcaag cacgcgcgtg cgctcaacgc
     1441 catcaccaac ccgggcacga acagctacaa gcgcctggtg ccgggcttcg aggcgccggt
     1501 caagctggct tacagctcgc gcaaccgctc ggcctcgatc cgcattccct acgtgccgaa
     1561 ccccaagggt cgccgcatcg aggcgcgctt ccccgatccg caggccaatc cttatctcgc
     1621 gttcgcggcc ttgctgatgg cgggtctcga cggcgtcgag aacaagatcc atcccggcga
     1681 agcggcgacc aaggacctgt atcacctgcc gccggaagaa gacgcgaaga tcccgaccgt
     1741 ctgctccagc ctcgaccagg cgctcgacta tctcgacaag ggccgcgcct tcctcaccaa
     1801 gggcggcgtc ttcaccgatg gctacatcga cgcgtacatt gaactgaaga tgcaggaggt
     1861 gacgcgcttc cggatgacga cgcatccggt cgagttcgac atgtactaca gcctctgatt
     1921 cgcagactcc cgacggcggg ggcgcttcgg cgtccccgcc gtgtttcttt accctgttcc
     1981 gccggcgatc gtgcgatcgt tcaatgggat cgaacgcaaa cctgcgagac aatcgccccc
     2041 gcctgtcggc gcgttgtccc gcgctggcgt tgacaggctg gagacgacct catgagcatg
     2101 cgaccttcca ttccttgcgc tgccgccgcg cttctagcgg cgatgctcgt cggccccggc
     2161 gtcgcccggg cccaggcttc ggcgtccgaa ggcagcgtca cgtatcgctg tcctggcaac
     2221 gactacaaga acaccatttc cgccaaggaa gcggagcgcc tgggctgcaa gaagctcgag
     2281 ggcgtgccga tcacgatcat ccagacttcc aggcctcgcc aggcgtcgcc ggcgggtgcg
     2341 ggcagctcca acgcgcgggt cgacccgcag gcgcagcgcg cccgggacac cgatgcccgt
     2401 cgcatcctcg agcaggagct gaagtcggag gaagaccgtc tcgccggcct gaagaaggac
     2461 ttcaacaacg ggcagccgga gcgccacggc aacgagcaga actaccagaa atacgtcgac
     2521 cgggtcgccg atctgagcgc ggcgatcgcc cgcaaggaag gcgacatcgc ggcgatcaag
     2581 cgcgagatcc agaagttgcc gccggcgcct ccgcagccgg tcctgtgacc
//
LOCUS       DASRWS010000320         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_131888, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000320 DASRWS010000000
VERSION     DASRWS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131888"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="trpB"
                     /locus_tag="VFF72_05995"
     CDS             <1..1172
                     /gene="trpB"
                     /locus_tag="VFF72_05995"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZV92749.1"
                     /translation="FGPYGGVFVPETLVTALDQLNAEYARAKNDPAFQQEFDHYLREF
                     VGRPSRLYFAKRLTEKLGGAKIYLKREDLNHTGAHKINNTIGQAMLARRMGKPRVIAE
                     TGAGQHGVATATICARYGLECVVYMGSEDIRRQAPNVYRMKLLGAEVVPVESGSKTLK
                     DALNEALRDWVTHVESTFYIIGTVAGPHPYPTMVRDFQRVIGEECIVQMNESIGRQPD
                     AVIACVGGGSNAMGIFHPYIGHAGTRLIGVEAAGEGLSSGRHAASICAGSPGVLHGNR
                     TYLLQDDDGQIALTHSISAGLDYPGVGPEHAWLKDIGRAEYVAVGDDEALAAFHRLCR
                     CEGIIAALESSHAVAYAMQLAPTMPVDRHLLVNLSGRGDKDIATVAELDARGRSA"
     gene            1248..2048
                     /gene="trpA"
                     /locus_tag="VFF72_06000"
     CDS             1248..2048
                     /gene="trpA"
                     /locus_tag="VFF72_06000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZV92750.1"
                     /translation="MRRIDAAFARLREQRRKALIPYVTVGDPHPSVTVDVMRAMAAAG
                     ADVIELGVPFSDPMADGPVIQKASERALAHGVGLRQVLGIVREFRAADSTTPVVLMGY
                     ANPIERYGIARFVDDAATSGVDGVLVVDYPPEECVDFIDNLRRRGLDPIFLIAPTSSD
                     ERIVAVAGMASGYVYHVSLKGVTGAGHFDIDAVKAMIPRIRQHVKLPVGVGFGIRDAK
                     TARAVADVADAVVIGTALVQRLETAGRDRAARAAGEFVGEIRAALDAR"
     gene            2068..2940
                     /gene="accD"
                     /locus_tag="VFF72_06005"
     CDS             2068..2940
                     /gene="accD"
                     /locus_tag="VFF72_06005"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZV92751.1"
                     /translation="MSWLEKLLPPKIQPTDPSERRTVPEGLWIKCPACETVLYKTDLE
                     KNIYVCPKCSHHHRIGARERLDAFLDAEGRYEIGQEVLPVDALKFKDSKKYPERLKDA
                     LENTGETDALIVMGGALMTLPVVAVCFEFDFMGGSMGSVVGERFVRGVHTAIEQKNAF
                     VSFTATGGARMQEGLLSLMQMAKTNAALTHLAKAGLPYISVLTDPTMGGVSASFAFVG
                     DVVIAEPKALIGFAGPRVIENTVREKLPDGFQRAEFLLQTGAVDMIVDRRELRPVIAR
                     QIAMLQRLPSDAVA"
     gene            3079..4377
                     /gene="folC"
                     /locus_tag="VFF72_06010"
     CDS             3079..4377
                     /gene="folC"
                     /locus_tag="VFF72_06010"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZV92752.1"
                     /translation="MDVSRTLDEWLAHCERLHPRTMQLGLDRVEAVRARLGLRFDSPL
                     VIVGGTNGKGSTCAMLESIATAAGYRTGLFSKPHLVRFEERCRVGGQMVDAAALVPHF
                     EAVEAARDGVALTYFEFTLLAIARLLASRELDLVILEVGLGGRLDAVNVFDADCAVLT
                     SIDVDHADILGADREAIGREKAGIFRRGRPAIVADPLPPRSVVDEARAIGADLWRAGI
                     DFRHSGDRQQWEWSGRETRFAGLGYPALRGANQLLNASAVLASLETLRHTLPASAQAV
                     RSGLASVDLPGRFQVVAGQPALVLDVAHNPHAAAALAHNLDRMGFFPRTHAVFGAMRD
                     KDIAAILARLGPIVDAWHFTNLPGARAANAAEIAAIQAAVATPGHPVSVALHTTPADA
                     LAAAVAAADPVDRIVVFGSFLTVGGVLASGLPQRSSPHLP"
     gene            4445..5287
                     /locus_tag="VFF72_06015"
     CDS             4445..5287
                     /locus_tag="VFF72_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZV92753.1"
                     /translation="MFKQKSEPTATPAPADPVQQARARARQRLIGAVVLLAIGIIGFP
                     LVFETQPRPIPVDIPIEIPRKDAVPPLVAPPAPLDAQRVGPGAASAAGHDGVITESQG
                     ESGRDVTPADRVAAAKPRVVVPAAPASTARAVTAPAMSSSQEPSSAAAMERTAAIPPA
                     ASRQEPAREEKEKTRAEASRVERERAEAARAKALLEGRSVAAAAASRYVVQVGAFADA
                     QAAREVRQKVEKLGLKTYTQIAQTSAGSRIRVRVGPFASRDEADAALAKAKAAGLAAV
                     VVTL"
BASE COUNT          808 a         1858 c         1804 g          828 t
ORIGIN      
        1 attttgggcc gtatggcggg gtgtttgtcc ccgaaacgct ggtcacggcg ctcgatcagc
       61 tcaacgccga atatgcccgc gccaagaacg acccggcttt ccagcaggag ttcgaccact
      121 atctgcgcga atttgtcggc cggccttcgc ggctctattt cgccaagcgc ctcaccgaaa
      181 aattgggcgg cgcgaagatc tacctcaagc gcgaagacct caaccacacc ggcgcgcaca
      241 agatcaacaa caccatcggc caggcgatgc tcgcccggcg catgggcaag ccgcgcgtca
      301 tcgccgagac gggcgccggt cagcacggcg tcgccacggc gaccatctgt gcccggtacg
      361 ggctcgaatg cgtcgtctac atgggctccg aggacatccg tcgccaggcg ccgaacgtct
      421 atcgcatgaa gctgctcggc gccgaggtcg tgccggtgga aagcgggtcg aagacgctga
      481 aggatgccct caacgaggcg ctgcgcgact gggtcactca cgtcgagagc accttctaca
      541 tcatcggcac cgtcgccggc ccgcatccgt atccgacgat ggtgcgcgat ttccagcgcg
      601 tcatcggcga ggagtgcatc gtccagatga acgagtcgat cggccgccag cccgacgccg
      661 tgatcgcctg cgtcggcggt ggcagcaacg cgatgggcat cttccatccc tacatcggcc
      721 acgccgggac gcgcctgatc ggcgtcgagg cggccggcga gggattgtcg agcggtcgcc
      781 acgccgcgtc gatctgcgcc ggcagccccg gcgtgctgca cggcaaccgc acctatctgc
      841 tgcaggacga cgacgggcag atcgccctga cccactcgat ctcggccggg ctcgactatc
      901 ccggcgtcgg cccggagcac gcctggttga aggacatcgg ccgggccgag tacgtggccg
      961 tcggcgacga cgaggccctt gcggcgttcc atcggttgtg ccgctgcgag ggcatcattg
     1021 cggccctcga atcgagccat gcggtcgcct acgcgatgca gctggcgccg acgatgcccg
     1081 tcgacaggca tttgctcgtc aatctctccg gccgcggcga caaggacatc gccacggtcg
     1141 ccgagctcga cgctcgcggg cggtcggcgt gagcgccttg gagtcgtcga tgcgagccgg
     1201 ttgcctcgtc ccgttggcag ccgccgcgcc gatccacggg tgaaacgatg aggcgcatcg
     1261 acgcggcgtt tgcacgcctt cgcgagcagc ggcgcaaggc gctaatcccg tacgtcaccg
     1321 tcggcgatcc gcatccgtcg gtcaccgtcg acgtgatgcg tgcgatggcc gccgccggcg
     1381 ccgacgtcat cgagctgggc gttcccttca gcgatccgat ggccgacggc ccggtcatcc
     1441 agaaggcgag cgagcgggcg ctcgcccatg gcgtcggcct gcgacaggtg ctcggcatcg
     1501 ttcgcgaatt ccgcgccgcc gactccacga ccccggtggt tctgatgggc tacgcgaatc
     1561 cgatcgaacg ctacgggatc gcgcgttttg tcgacgacgc cgcgacgtcc ggcgtcgacg
     1621 gcgtcctcgt cgtcgactat ccgcccgagg aatgcgtcga cttcatcgac aacctgcggc
     1681 gtcgcggtct cgacccgatc tttctcatcg ccccgacctc gagcgacgag cgcatcgtcg
     1741 cagtcgccgg gatggcctcg ggttacgttt accatgtgtc gctgaagggc gtgaccggtg
     1801 ccggacactt cgacatcgac gccgtgaaag cgatgattcc ccgcatcagg caacacgtga
     1861 agctgcccgt cggcgtcggc ttcggcatcc gcgacgcaaa gacagcgcgg gccgtcgccg
     1921 acgtggccga cgccgtcgtc atcggcacgg cgctggtgca gcgcctcgag acggctggcc
     1981 gtgaccgcgc ggcgcgggcg gccggggagt tcgtcggcga gatccgggcc gctctcgatg
     2041 cccgataaga gaacaggaaa cgatgcgatg agctggctcg agaaactcct tcccccgaag
     2101 atccagccga ccgatccgag cgagcggcgg accgtccccg agggcctgtg gatcaagtgt
     2161 ccggcgtgcg agacggtgct ctacaagacc gatctcgaaa agaacatcta tgtctgtccg
     2221 aagtgcagcc accatcaccg gatcggcgcg cgcgagcggc tcgacgcgtt cctcgacgcc
     2281 gaaggccgct acgagatcgg ccaggaggtg ctgccggtcg acgccctcaa gttcaaggac
     2341 agcaagaagt atcccgagcg gttgaaggac gcgctcgaga acacgggcga gaccgatgcg
     2401 ctcatcgtca tgggcggcgc cctcatgacg ctgccggtgg tcgccgtctg cttcgaattc
     2461 gatttcatgg gcggctcgat ggggtcggtc gtgggcgagc gtttcgtgcg cggcgtgcac
     2521 accgcgatcg agcagaagaa tgccttcgtg agcttcaccg cgacgggcgg cgcgcgcatg
     2581 caggagggct tgctcagcct gatgcagatg gccaagacca atgcggcgct gacgcacctg
     2641 gcgaaggccg gccttccgta catcagcgtg ctgacggatc cgacgatggg cggcgtgtcg
     2701 gcgagcttcg cgttcgtcgg cgatgtggtc atcgccgagc ccaaggcatt gatcggattc
     2761 gccgggccga gggtgatcga gaacaccgtt cgcgagaagc ttccggacgg cttccagcgc
     2821 gccgagttcc tgctgcagac cggggcggtc gacatgatcg tcgaccggcg cgagcttcgg
     2881 ccggtgatcg ccaggcagat cgcgatgctg cagcggctgc cgtccgacgc ggttgcttga
     2941 cggagccccc acgctcacga gtacgttcgc tgccccccga gggggcgcgg ccggccttgg
     3001 gacggcccgg cgcccggccg gagcccctac gctcacgggt acgttcgctg cccggccaag
     3061 gggccgagga cggcggcgat tgacgtgagc aggacgctcg acgaatggct cgcgcactgc
     3121 gagcgcctgc atccgcggac gatgcaattg ggcctcgatc gcgtcgaggc cgtgcgcgcg
     3181 cggctcgggc tgcgcttcga ctcgccgctc gtcatcgtcg gtggcaccaa cggcaagggt
     3241 tcgacctgcg cgatgctcga gtcgatcgcg acggcggcgg gctaccgcac gggtcttttt
     3301 tcgaagccgc atctggtccg tttcgaggag cgatgccggg tcggcggaca gatggtcgac
     3361 gccgcggcgc tggtgccgca tttcgaggcg gtcgaagcgg cgcgcgacgg cgtcgcgctc
     3421 acctacttcg agttcacttt gctggcgatc gcccggctcc tcgcgtcgcg agagctcgac
     3481 ctcgtcatcc tcgaagtggg cctcggcggc cgcctcgacg ccgtcaacgt cttcgatgcc
     3541 gactgtgccg tcctcacgag catcgacgtc gatcatgccg acatcctcgg cgccgaccga
     3601 gaagcgatcg gccgcgagaa ggcgggcatc ttccgccgcg gtcggccagc catcgtcgcc
     3661 gacccgctgc cgccacgcag cgtcgtcgac gaggcacgcg cgatcggcgc cgacctgtgg
     3721 cgtgccggaa tcgatttccg tcactcaggc gaccgccagc agtgggagtg gtcgggccgc
     3781 gagacccgct ttgccggcct cggctacccc gcgctgcgcg gcgccaatca gcttctcaat
     3841 gcgtcggcgg tgctggcgtc gctcgaaacg ctgcgccaca ctctgcccgc gagcgcccag
     3901 gcggtgcgca gcggcctggc gagcgtcgat ctgccgggcc gcttccaggt cgtcgccggc
     3961 cagccggcgc tcgtcctcga cgtcgcccac aacccgcacg cggccgcggc gctcgcccac
     4021 aacctcgacc ggatggggtt ctttcctcgt acccatgcgg tattcggggc gatgcgcgac
     4081 aaggacatcg ccgcgatcct cgcgcgcctg gggccgatcg tcgatgcctg gcacttcacc
     4141 aatctgccgg gcgcgcgcgc ggccaacgcg gccgagatcg cggcgatcca ggcggccgtc
     4201 gcgaccccgg gtcacccggt gtccgtcgcc ctgcacacga cgcccgccga cgcgctggca
     4261 gcggccgtcg ccgctgccga cccggttgat agaatcgtcg tcttcggatc cttcctcacc
     4321 gtcggtggcg tcctcgcgag cgggctgccg caacgcagca gtccgcactt gccttgagag
     4381 tcccggtcgt ttcgcttccg gcgttgtcgc caccccccgc gtccggctcg aactcgaggc
     4441 cctgatgttc aagcagaaga gcgagcccac cgcgacaccg gctcccgccg atcctgtcca
     4501 gcaggcgcgc gcgcgcgcgc ggcagcggct catcggcgcg gtggttcttc tggccatcgg
     4561 catcatcggt tttcctctcg tcttcgagac gcaaccgcgg ccgattccgg tcgacatccc
     4621 aatcgagatt ccgcgcaagg acgccgttcc gcctctggtg gcgccgcctg cgcccctcga
     4681 cgcgcaacgt gtcggcccgg gcgccgcgtc ggctgccgga cacgatggcg tcatcaccga
     4741 atcgcagggc gagtccggac gcgacgtcac tccggcagac cgtgtcgccg cggcgaagcc
     4801 tcgcgtcgtc gttccggccg cgcccgcatc gacagcgcgc gcggtcacgg cgccggcaat
     4861 gtcgtcctcg caggagccgt cgtcggctgc ggcaatggag cggaccgcgg ccattccgcc
     4921 ggccgcatcg cgtcaggagc ccgcgcgcga ggaaaaggag aagacccgcg ccgaggcatc
     4981 ccgcgttgaa agagaacgcg ccgaggcggc gcgcgccaaa gcgcttctcg agggccggag
     5041 cgtggccgct gccgcggcgt ccagatatgt cgtgcaggtc ggcgctttcg ccgacgcgca
     5101 ggccgcgcgc gaggtgcgcc agaaggtcga aaagctcggc ctgaagacct acacgcagat
     5161 cgcgcagacg tcggcgggca gccgcatccg cgtccgcgtc ggccccttcg cgtcgcgcga
     5221 cgaggccgac gctgcgctcg ccaaggcgaa ggcggccggg ctggcggccg tcgtcgtcac
     5281 gctctgagcc gtgtcatg
//
LOCUS       DASRWS010000321         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2420447, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000321 DASRWS010000000
VERSION     DASRWS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420447"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VFF72_06020"
     CDS             complement(<1..1221)
                     /locus_tag="VFF72_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HZV92754.1"
                     /translation="MDRLNRLGLLFGVVLAATTVVGCGGGDDSSPAPAPAPTPAPPPP
                     PPAAVALLNNPDPASGCAALAPLASTLSATGAQVAITSATLQATTTPDANGNYQPYCA
                     VVGTINGNRQGTQLAGQTTAQTTYAIGFQVNLPSQWNGKLFFSGGGGTDGSIPNTTGT
                     VTAGESVNPLLLGYATASDDSGHNASNNDNNNAGGAAFGLDEQARLDFGYNAIGLTKT
                     LANGLISSYYAKAQTHSYFVGCSEGGREAMMVSQRFGGEFDGVVAGDPGSDLPKAWLA
                     EAWNTQQFGAAAAAQGYFETSGPGAGTTPLLAAAVQPAQWTALQQAILSQCDAADGLV
                     DGMVNKTCNFDPTVLACGAAGAPAACWLPAQLTAIENVIAGPKDSAGNVLYSDWLWDP
                     GIGQGGWNVWNTGFF"
     gene            1454..2233
                     /locus_tag="VFF72_06025"
     CDS             1454..2233
                     /locus_tag="VFF72_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZV92755.1"
                     /translation="MIASKALLRIDHRGAVTHVRLVRPAKRNAINDALVAELHDAFAK
                     LPKTTRVVVLSAEGDHFCAGLDLSELGERSTAEGIWHSRTWHAAFEQIQFGQVPVIAV
                     LHGAVVGGGLELASACHIRIAEPSTFYGLPEGSRGLFVGGGGSARIPRLMGVARMADM
                     MLTGRVYDAEEGHAAGLSQYLVGAGEGLAKAEAMAGKIATNAPLSNFAILHALPRIAE
                     MGQNEGLFAESLMAAIAQGDGAAKDRMRAFVEGRAAKVAKS"
     gene            2337..>2610
                     /locus_tag="VFF72_06030"
     CDS             2337..>2610
                     /locus_tag="VFF72_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346026.1"
                     /note="catalyzes the conversion of ferulic acid to
                     feruloyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HZV92756.1"
                     /translation="MTAAAAHVEAPYRALPLGGSLSASFEERGDGSTIVRSTEPLAEY
                     PRRITDRLLHWAEMVPDRTLVAKRVRNADGTRGDWRRVSYAEALATA"
BASE COUNT          396 a          870 c          913 g          431 t
ORIGIN      
        1 gaagaaaccg gtgttccaga cattccagcc gccctggccg atacccgggt cccacagcca
       61 gtcggaatag agcacgttgc cggcggagtc cttcggtccg gcgatcacgt tctcgattgc
      121 cgtcagctgg gcaggcaacc agcaggccgc cggcgcgccc gcggcgccgc aggcgagcac
      181 ggtcgggtcg aagttgcacg tcttgttgac catgccgtcg accaggccgt cggccgcgtc
      241 gcactgcgac aggatcgcct gctgcaaggc cgtccattgc gccggttgaa ccgccgcagc
      301 cagcaggggc gtcgttccgg cgcccgggcc gctcgtttcg aagtagccct gtgcggcggc
      361 agcggccccg aactgctgcg tattccatgc ctcggccagc caggccttcg gcaggtcgga
      421 gccaggatcg ccggcgacga cgccgtcgaa ctcgccgccg aagcgctgcg acaccatcat
      481 tgcctcgcga ccgccttccg agcagccgac gaagtacgaa tgcgtctgcg ccttggcgta
      541 gtagctgctg atgaggccgt tggcgagcgt cttggtcagg ccgatggcgt tgtagccgaa
      601 gtcgaggcgg gcttgctcgt cgaggccgaa ggcggccccg ccggcgttgt tgttgtcgtt
      661 gttgcttgcg ttgtggccgc tgtcgtccga ggccgttgcg tagccgagga ggagcggatt
      721 caccgattcg ccggcagtga ccgttccggt ggtgttcggg atcgagccgt cggtgccgcc
      781 gccgccgctg aagaagagct tgccgttcca ctggctcggc aggttcacct gaaagccgat
      841 ggcataggtc gtctgcgcgg tcgtctggcc ggcgagctgg gtgccctgcc ggttgccgtt
      901 gatcgtgccg accacggcgc agtagggctg gtagttgccg ttggcgtcag gcgtggtcgt
      961 cgcctgcaag gtcgccgagg tgatcgccac ttgggcgccc gtcgccgaga gcgtgctcgc
     1021 caatggggcg agagcggcgc agccgctcgc cggatcggga ttgttgagca gtgcgacggc
     1081 tgcaggcggc ggcggcggtg gtgccggagt cggggccggt gccggcgctg gcgaggaatc
     1141 gtcgccgcct ccgcacccga cgacggtggt cgcggccagc acgacgccga acagcaaccc
     1201 cagacggttc agtcgatcca tgtttctctc ctcggttgga acggcggcat ctccgttggc
     1261 cgctgtcttg cgatcaatgt agttgccgga gttaatcgtt aatctcggta aataccatga
     1321 gggagatgtg atagcgaaat cgttttggaa cataactatg cagagaccac gaggcgcatc
     1381 aggggttccc cctagaggtc ggcggagcgc cacttctaga attcgcgccc ggcatggtta
     1441 aaacggctaa tcaatgatcg cgtccaaggc gctgcttcgc atcgatcatc gcggtgccgt
     1501 cacgcacgtc cgcctggtcc gtcccgccaa gcgcaacgcc atcaacgacg ccctcgtcgc
     1561 cgaactgcac gacgccttcg ccaagctgcc gaagaccacg cgcgtggtcg tcctcagcgc
     1621 agaaggcgac cacttctgcg ccggcctcga tctgtccgag ctcggcgagc gaagcaccgc
     1681 cgagggcatc tggcattcgc gcacctggca cgccgccttc gagcagatcc agttcggcca
     1741 ggtccctgtg atcgccgtgc tgcatggagc cgtcgtcggc ggcgggctcg agctggcgag
     1801 cgcctgccac atccgcatcg ccgagccgag cacgttctac ggcttgcccg agggctcgcg
     1861 aggcctcttc gtcggtggcg gcggctcggc gcggatcccg cggctcatgg gcgtcgcccg
     1921 gatggccgac atgatgctca ccggccgcgt ctacgacgcc gaagaaggcc acgcggcggg
     1981 cctctcgcaa tacctcgtcg gtgccggcga agggctggcc aaggccgagg ccatggccgg
     2041 gaagatcgcg accaacgcgc cgctgtcgaa cttcgcgatc ctgcatgcgt tgccgcggat
     2101 cgccgagatg ggccagaacg agggcctgtt cgccgagtcg ctgatggccg cgatcgctca
     2161 aggcgacggt gccgccaagg accgcatgcg ggccttcgtc gagggccgcg ccgcgaaggt
     2221 ggcgaaatcg tgatcgcgag cgtcgtgcct ggatcgaacg accgcgaccg gtgcggcgtc
     2281 gatcggatcg gcaagctcgc tgtccggcgg ctgcgcatgg cctcgggaga ttcacgatga
     2341 cggcggccgc ggcgcatgtc gaggcaccgt accgcgcgct gccgctgggt ggctcgctca
     2401 gcgccagctt cgaggagcgc ggcgacggca gcaccatcgt ccgctcgacc gagccgctcg
     2461 ccgaatatcc gcgccggatc accgaccgcc tcctgcactg ggccgagatg gtgcccgatc
     2521 gcaccctggt cgccaagcgg gtgcgcaacg ccgacggcac gcggggcgac tggcgccgcg
     2581 tcagctatgc cgaagcgctg gccacggcgc
//
LOCUS       DASRWS010000322         8215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2420679, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000322 DASRWS010000000
VERSION     DASRWS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420679"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFF72_06035"
     CDS             <1..1293
                     /locus_tag="VFF72_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZV92757.1"
                     /translation="LIASDAAYWRLGRETYIKPPEMPLRFNTGMHWGCPYDCGLCPDH
                     MQHSCLTIVEITDHCNLACPVCYAESGPHRQVHRDLATVERMLDAIVANEGEADVVQL
                     SGGEPTLHPQIFEILDAARRRPIRHLMLNTNGIRIANEAGFAERLAEYPPGFEVYLQF
                     DSFERDALMTLRGSDLRRIRERALENLNRVGLSTTLVMTIARGVNDGEVGRLIEYAAQ
                     QPCVRGVTLQPIQFAGRTDGVTPAGQRLTLTEVRQSILDQSAFFTAADIIPVPCNPDA
                     LAMGYALKTAAGIVPLTRFVGPEVLLAGDRNTIVFESEPALKDAVFKLFSTNLGPESQ
                     ATCLSSLMCCLPQVQAPRIGYDSVFRVLVMAFMDAVDFDVRAMKKSCVHIVQPDGRII
                     PFEAFNLLYRDGKATRLRQLRERVEAGWADAFDPVHPA"
     gene            1344..1574
                     /locus_tag="VFF72_06040"
     CDS             1344..1574
                     /locus_tag="VFF72_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92758.1"
                     /translation="MRIVWIVFMGLAGLVGLGMSLCGGVFLLSTMSDPNGLMFIALGS
                     LAVGALICFGAWRGLVGRPPPPDNAREIDRQA"
     gene            complement(1610..3055)
                     /locus_tag="VFF72_06045"
     CDS             complement(1610..3055)
                     /locus_tag="VFF72_06045"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZV92759.1"
                     /translation="MDARVYPNTQLFIDGTWQNAAAGRTMAVVNPASGDNIGTLAFAE
                     QADLDHALEAADKGFSTWKRISAFERSKMMRKAANLLRERVDHVAMLMTMEQGKTLAE
                     AKVEIMNGADTIDWFAEEARRAYGRVIPARGEGIYQLVVKEPVGPVAAFTPWNFPINQ
                     IVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAGVPPGTIGLVYGDPAAISSYL
                     IPHPVIRKISFTGSTVVGKQLAAMAGAHMKRATMELGGHAPVIVFDDADVTIAAKAMA
                     ASKFRNAGQVCISPTRFLVQEGVYDSFVEQFVANAKKLKVGDGREQGVNMGALANPRR
                     SKAMDVNLEDATKHGAKVRTGGHRIGAKGNFFEPTVVTELPNDAKAMNEEPFGPMAII
                     NPFKTFDDAVKEANRLPFGLAAYAWTKSAKTANAIANAVETGMISINHVGLGIPEVPF
                     GGVKDSGYGSEGGSEAIEPYLNPKFVTQAGL"
     gene            3180..3977
                     /locus_tag="VFF72_06050"
     CDS             3180..3977
                     /locus_tag="VFF72_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HZV92760.1"
                     /translation="MKNRIAFLAAGAALLAAAGQASAQVTFYEGPGFHGRAFTADHPI
                     GNLARFGFNDRASSAVVDHGRWEVCDDAHFRGRCVVLRRGSYDSLRSMGLDNRISSVR
                     PVSRQGVGVEAPPPPPPQPVYEYRQRPNERLFQATVRDVHAVVGPPERRCWVEREQVA
                     QAPAGPNVGGAIIGGVLGGILGHQIGGGSGRTATTIAGAVGGTALGANVGSAPATRDV
                     RRCSTAPSGPPDYWDVTYDFRGVEHHVQMSAPPGPTITVNGAGEPRQ"
     gene            complement(4013..4222)
                     /locus_tag="VFF72_06055"
     CDS             complement(4013..4222)
                     /locus_tag="VFF72_06055"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HZV92761.1"
                     /translation="MTHESDAAEDPSSEAMVDFASEASIVRWARALGTTDEALMSAIQ
                     AVGARVDRIKEYLGEGGDADEQIDG"
     gene            complement(4246..4965)
                     /locus_tag="VFF72_06060"
     CDS             complement(4246..4965)
                     /locus_tag="VFF72_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV92762.1"
                     /translation="MILDNGQVPLYVQLADLFRQRIHKGIWKKGEKLPSLEKLVEEFS
                     VARVTVRQAVDRLTRDGLVSPQRGRGTFVTGAPEANRWLRVETTLRNLADVYRDTSPT
                     ILNIDESTRSPRLLEADGVAAPKYVYMRRVHSREGRPYCVIDIYLDAEIFARHPQRFR
                     RETVIPLITEMPGVRVVTARQVLTISTADLEVARHLGIRVNAPVADVRRVFTGPKRRV
                     IYLGEVTYRGDFVHVEFDLKS"
     gene            complement(4962..6623)
                     /locus_tag="VFF72_06065"
     CDS             complement(4962..6623)
                     /locus_tag="VFF72_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZV92763.1"
                     /translation="MDDEAPGGFDYIVVGAGSAGCVLANRLSADPARRVLLIEAGGEA
                     RNFWLRLPVGYFKTIYDPRFSWQFALEPEASTDHRAIVWPRGKALGGSSVINGLLYIR
                     GQHEDFDDWSGINGARGWSHRELLPFFKRSERYEGGESEYHGGHGELGVSDLRNDHPY
                     CEAWLDAAEQAGFDRTDDFNGARSAGVGRYQLTLRGHWRCDAATAFLAPARGRANLVV
                     ATGAQATRLLVEHGRCAGVAYVQGGQEREARADAEVIVACGALQSPQLLQLSGIGPAA
                     LLGEHGIDVVADSADVGANLQDHYQARVIVKLKARRSLNDDVRNPLRLAQMGAQWLLQ
                     ARGPLTVGAGQVGGLVASAHAVGGRPDLLFNVMPLSVDKPGDPLHRFSGFSASAAQCR
                     PESRGRVQLASADPLAPPRIAANYLRESRDVKVLVSGLEILRDIFARPAFRGLVTGEE
                     YLPGRGVTTADALERFARAKGGTVFHPTSTCRMGGDARSVVDERLRVRGVDRLRVVDA
                     SVMPRIVSTNTNAAAIMIGERGAAMILEDAGDVRAVVSPALQAAA"
     gene            complement(6843..7838)
                     /locus_tag="VFF72_06070"
     CDS             complement(6843..7838)
                     /locus_tag="VFF72_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92764.1"
                     /translation="MTIRRRALLQATAASLATPSLAAFAQAKTKVRVGYLHTPAVDGQ
                     IWIGEHSGTWARHGLDLEMIKFTTGLELFQAMIGGSLDVLSTGAVVSNFPARGQGKVF
                     LINDIEYATAQLWVRDPSIKTVADLKGKQISTTTGTTAHVFLDLALRDAGLDPNKDVH
                     IVNQRMAEAVTSFVSGAVPAVALWVPFDQTVRKQVPGARKISDASKFFPKAAIVDGWA
                     ARNDYFDDHKPVLEKIIAAWAEVNDAMLANFDAAAKTLQETQYKEIPLADFKEAMSYS
                     KYYSSAEWRKLYVDGTVTKWLQQVTDFFVKVGNIQSPVAASTYFDPQLYEKVIKG"
     gene            complement(7892..>8215)
                     /locus_tag="VFF72_06075"
     CDS             complement(7892..>8215)
                     /locus_tag="VFF72_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV92765.1"
                     /translation="MDEPFGALDAQTRSAMQDLLLQVLAAEGKTVLLITHSVDEAIYL
                     SSRIVVVTARPARVRTVIEVPFGYPRSEKVHEDPRFAALRARIRSLVMEEYEAQARQA
                     VRVVD"
BASE COUNT         1241 a         2819 c         2810 g         1345 t
ORIGIN      
        1 ctgatcgcat cggacgccgc gtactggcga ctcggccgcg agacctacat caagccgccc
       61 gagatgccgc tgcgcttcaa caccgggatg cattggggct gcccctacga ctgcggcctg
      121 tgtcccgacc acatgcagca ctcgtgcctg acgatcgtcg agatcaccga ccactgcaac
      181 ctcgcctgcc cggtctgcta cgcggagtcg ggcccgcatc ggcaggtcca ccgcgacctg
      241 gcgacggtcg agcgcatgct cgacgccatc gtcgccaacg agggcgaggc cgacgtcgtc
      301 cagctctccg gcggcgagcc gacgctgcat ccgcagatct tcgagatcct cgatgccgcg
      361 agacgtcggc cgatccgcca tttgatgctc aacaccaacg gcatccggat cgccaacgag
      421 gccggcttcg ccgagagatt ggccgagtac ccgccggggt tcgaggtcta cctgcaattc
      481 gactcgttcg agcgcgacgc cctgatgacg ctgcgcggat cggacctgcg caggatccgc
      541 gagcgggcgc tggaaaacct caatcgcgtc ggcctgtcga cgaccctggt catgacgatc
      601 gcgcgcggcg tcaacgacgg cgaggtcggc aggctgatcg aatacgccgc gcagcagcct
      661 tgcgtgcgcg gcgtgacgtt gcagccgatc cagttcgcgg gccgcaccga cggcgtcacg
      721 ccggccgggc agaggctgac cctcaccgaa gtgcgccagt cgatcctcga ccagtccgcg
      781 ttctttaccg ccgccgacat catcccggtg ccctgcaacc ccgatgcgct cgcgatgggc
      841 tacgcgctga agaccgccgc cggcatcgtg ccgctgacgc gcttcgtcgg gcccgaggtg
      901 cttctggcgg gagaccgcaa caccatcgtc ttcgaatccg agccggcctt gaaggacgcg
      961 gtgttcaagc tcttctcgac gaatctcggc ccggagagcc aggcgacatg cctgtcgtcg
     1021 ctgatgtgct gcctgccgca ggtgcaggcg ccacgaatcg gctacgacag cgtctttcgc
     1081 gtcctggtca tggccttcat ggacgcggtc gatttcgacg tgcgggcgat gaagaagtcg
     1141 tgcgtgcaca tcgtccagcc cgacggcagg atcattccct tcgaggcgtt caacctgctc
     1201 taccgggacg gcaaggcgac gaggctgagg caacttcgcg aacgcgtcga ggccggctgg
     1261 gccgacgcgt tcgatcccgt ccatccggcg taacgcgtcg tccggcgtcg tcgagcgata
     1321 cttccaccca cgtctcaccc gtcatgcgaa tcgtctggat cgtcttcatg ggcctggccg
     1381 gattggtcgg gctgggcatg tcgctttgcg gcggcgtctt cctgctctcg acgatgtcgg
     1441 acccgaacgg gctcatgttc atcgccctgg gctcgctggc cgtgggcgcc ctgatctgtt
     1501 tcggtgcgtg gcgcggtctg gtcggtcgtc cgccgccacc cgacaacgcg cgggagatcg
     1561 atcgccaggc ctgatctgcg gcggacgcgt cgcgacgtcc gcgcccttct cagagtccgg
     1621 cctgcgtgac gaacttcgga ttgaggtaag gctcgatcgc ctcgctgccg ccctcggaac
     1681 cgtagcctga atccttgacg ccgccgaacg gcacttcggg aatgccgagg ccgacgtggt
     1741 tgatcgagat cattcccgtc tcgaccgcgt tggcgatcgc gttggcggtc ttcgccgact
     1801 tcgtccacgc gtaggcagcc agcccgaacg gcaggcggtt cgcttccttg accgcgtcgt
     1861 cgaaggtctt gaacggattg atgatcgcca tcggcccgaa cggctcctcg ttcatcgcct
     1921 tggcgtcgtt gggcagctcg gtgacgacgg tgggctcgaa gaagttgccc ttcgcgccga
     1981 tgcggtggcc gccggtcctg accttggcgc cgtgcttcgt cgcatcctcg agattgacgt
     2041 ccatcgcctt cgagcgccgt gggttggcca gcgcgcccat gttgacgccc tgctcgcggc
     2101 cgtcgccgac cttgagcttc ttcgcgttgg cgacgaactg ctcgacgaac gagtcgtaga
     2161 cgccttcctg gaccagaaag cgcgtcggcg agatgcagac ctggccggca ttgcggaact
     2221 tgctcgccgc catcgccttg gcggcgatcg tgacatcggc gtcgtcgaag acgatcaccg
     2281 gggcgtggcc gccgagctcc atcgtcgctc gcttcatatg cgcaccggcc atcgccgcga
     2341 gctgcttgcc gacgacggtg gaaccggtga acgagatctt gcggatcacc ggatgcggaa
     2401 tgagatagct cgagatcgcc gccggatcgc cgtagacgag gccgatcgtc cccggcggca
     2461 cgccggcgtc ggcgaaggcg cggatcagct cggcgggtga cgccggcgtt tcctcgggcg
     2521 ccttgacgat gatcgagcag ccggcggcca gcgccgccga gagcttcctc acgatctggt
     2581 tgatcgggaa gttccacggc gtgaacgcgg cgaccgggcc gaccggctcc ttgacgacga
     2641 gctggtagat gccttcgccg cgcgccggaa tgacgcggcc gtaggcacga cgcgcttcct
     2701 cggcgaacca gtcgatggtg tcggcgccgt tcatgatctc gaccttggcc tcggcgagcg
     2761 tcttgccctg ctccatcgtc atcagcatgg cgacgtggtc gacgcgctcg cgcagcaggt
     2821 tggcggcctt tctcatcatc ttcgagcgct cgaaggcgct gatccgcttc caggtcgaaa
     2881 agcccttgtc ggcggcttcg agcgcgtggt cgagatcggc ctgctcggcg aacgccagcg
     2941 tgccgatgtt gtcgccgctg gccgggttga cgaccgccat ggtgcgaccc gccgcggcgt
     3001 tctgccacgt gccgtcgatg aagagctggg tgttggggta gacgcgtgca tccatgggtc
     3061 acaggctcgc ttgaagacaa tccgtaacga tagcggtttc gcccggtcgg cgcggggccg
     3121 gggtcgcgcg ttgaaggtgg acgtgcccgg ccgggcactt cgtttgctgt ggagacgtca
     3181 tgaaaaaccg aatcgcattc ctcgccgccg gcgcggccct gctggctgcg gccggccagg
     3241 ccagtgccca ggtcacgttc tacgagggtc cgggctttca tggccgcgcc ttcaccgccg
     3301 atcatccgat cggcaacctt gcccgcttcg gcttcaacga ccgcgcctct tcggcggtgg
     3361 tcgatcacgg tcgctgggag gtgtgcgacg acgcgcactt caggggccgc tgcgtcgtgc
     3421 tgcgtcgtgg cagctacgac tcgctgcgca gcatgggcct cgacaatcgc atctcttcgg
     3481 tgcggccggt ctcgcgccag ggtgtcggcg tcgaggcgcc gcctccgcca ccgccgcagc
     3541 ccgtatacga gtaccgccaa cggccgaacg aacggctctt ccaggcgacg gtgcgcgacg
     3601 tgcacgccgt cgtcgggccg ccggagcgcc gctgctgggt cgagcgcgag caggtcgcgc
     3661 aggccccggc cggcccgaac gtcggcggcg ccatcatcgg cggcgtcctc ggcggcatcc
     3721 tcggccacca gatcggtggc ggcagcggcc gcaccgcaac gaccatcgcc ggtgccgtcg
     3781 gcggcaccgc gctcggcgcc aatgtcggca gcgcgccggc gacgcgcgac gtgcggcgct
     3841 gctcgaccgc gccgagcggc cctcccgact actgggacgt gacctacgac tttcgcggtg
     3901 tcgagcacca cgtgcagatg agcgcgccgc cggggccgac gatcaccgtc aacggcgccg
     3961 gcgagccgcg gcaatagcga ggacgacccg ccggtcgcat cggcgatcgc gatcagccgt
     4021 cgatctgctc gtcggcgtcg ccgccttcgc cgaggtattc cttgatccgg tcgacgcgcg
     4081 cgccgaccgc ctggatcgcc gacatgaggg cctcgtcggt cgtgccgagc gctcgcgccc
     4141 agcgcacgat cgacgcctcg ctggcgaagt cgaccatcgc ttcggatgaa ggatcctcgg
     4201 cggcatcgga ctcgtgggtc atggcccgca cattctgcat cggcctcagg acttgaggtc
     4261 gaactcgacg tgcacgaaat cgccgcgata ggtgacttcg cccagataga tcacccgccg
     4321 cttcggcccc gtgaagacgc gccgcacgtc ggcgaccggt gcgttgacgc ggatgcccag
     4381 gtgccgcgcg acctcgagat cggcggtcga gatcgtcagc acctggcgcg cggtgacgac
     4441 gcgcacgccc ggcatctcgg tgatcagcgg gatcaccgtc tcgcggcgga agcgctgcgg
     4501 gtgacgggcg aagatctcgg cgtcgagata gatgtcgatg acgcaatacg gcctgccctc
     4561 gcgcgaatgg acgcgtcgca tgtagacgta cttcggcgcg gccacgccat cggcctcgag
     4621 caggcgcggg ctgcgcgtcg actcgtcgat gttgaggatg gtgggcgacg tgtcgcgata
     4681 gacgtcggcg aggttgcgca gcgtcgtctc gacgcgcagc caccggttgg cctcgggcgc
     4741 gccggtcacg aaggtgcctc ggccgcgctg cggcgagacg aggccgtcgc gcgtcagccg
     4801 atcgaccgcc tggcggaccg tgacgcgggc gacgctgaac tcctcgacga gcttttccag
     4861 cgacggcagc ttctcgccct ttttccagat gcccttgtgg atgcgctggc gaaagagatc
     4921 cgcgagctgg acgtagagcg gaacctggcc gttgtcgagg atcacgctgc cgcctgcaac
     4981 gccggcgaga cgacggcccg cacatcgcca gcgtcctcga ggatcatcgc cgcgcccctc
     5041 tcgccgatca tgatcgccgc cgcgttggtg ttggtcgaga cgatgcgcgg catcaccgag
     5101 gcgtcgacca cgcgcagccg atcgacgccg cgaacgcgca gccgctcgtc gacgaccgaa
     5161 cgcgcatcgc cacccatccg gcacgtgctg gtcggatgga acaccgtgcc gcccttggcg
     5221 cgggcgaagc gctcgagcgc gtcggcggtg gtgacgccgc gtccgggcag gtactcctcg
     5281 ccggtgacca gcccgcgaaa cgccgggcgg gcgaagatgt cgcgcaggat ctcgaggccc
     5341 gagacgagca ccttcacgtc gcgcgattcg cgcaggtagt tggcggcgat gcgcggcggc
     5401 gcgagcggat cggccgacgc cagctgcacg cggcctctcg actcgggccg gcactgcgcc
     5461 gccgatgccg aaaagcccga gaagcggtgc agcggatcgc ccggcttgtc gaccgacagc
     5521 ggcatcacgt tgaagagcag atcgggccgg ccgcccacgg catgcgcgct cgcgaccaga
     5581 ccgccgactt ggccggcgcc gaccgtcaac gggccgcgcg cctgcaacag ccactgcgca
     5641 cccatctgcg ccaggcgcag cgggttgcgc acgtcgtcgt tgagcgatcg acgcgccttc
     5701 agcttgacga tgacgcgggc ctggtagtga tcctgcagat tcgcgccgac gtcggccgaa
     5761 tcggcgacga cgtcgatgcc gtgctcgccg agaagcgccg ccgggccgat gccggagagc
     5821 tgcagcagct gcggcgactg cagggcgcca caggcgacga tcacctcggc gtcggcacgc
     5881 gcctcgcgct cctggcctcc ctgcacatag gcgacgccgg cacagcggcc gtgctcgacg
     5941 agcagccggg tcgcctgcgc gccggtggcg acgacgaggt tggcccggcc acgtgccggc
     6001 gcgaggaacg ccgtcgccgc gtcgcagcgc cagtggcctc gcaaggtgag ctggtagcgg
     6061 ccgacgccgg ccgagcgggc gccgttgaaa tcgtcggtgc gatcaaagcc cgcctgctcg
     6121 gcggcgtcga gccaggcctc gcaatacgga tggtcgttgc gcaggtcgga gacgccgagc
     6181 tcgccgtggc caccgtggta ttcgctctcg ccgccctcgt agcgctccga gcgcttgaag
     6241 aacggcagca attcgcgatg gctccagccg cgcgcgccgt tgattcccga ccagtcgtcg
     6301 aagtcctcgt gctggccgcg gatgtagagc aggccgttga tgacgctcga gccgccgagc
     6361 gccttgccgc gcggccagac gatggcacga tgatcggtcg acgcctccgg ctcgagcgcg
     6421 aactgccacg agaagcgcgg gtcgtagatc gtcttgaagt agccgaccgg caggcgcagc
     6481 cagaaattcc gcgcctcgcc gcccgcctcg atcagcagca cccgacgtgc cggatcggcg
     6541 ctcagccggt tggccaggac gcagccggcc gagcccgcgc cgacgacgat gtagtcgaat
     6601 ccgcccggcg cttcgtcgtc catctcaatc ccgattcgac gatccagagt tggacgtccc
     6661 atccaggccg ccgccatgga tccggctgcg ccggtccatc ggcatgcgct ccctcgcaag
     6721 gcagcgaaca agagcgcgca tcggggctcc aaccaagcca ccgccatgga tccggctccg
     6781 ccggtccatc ggcgggcgcc ccctcggggg gcagcgagcg aaagcgagcg tgggggcccc
     6841 gttcacccct tgatcacctt ctcgtagagc tgaggatcga aataggtgga ggccgccacc
     6901 gggctctgga tgttgccgac cttgacgaag aagtcggtca cctgctgcag ccacttcgtc
     6961 accgtgccgt cgacgtagag cttgcgccat tcggccgagg agtagtactt cgagtagctc
     7021 atcgcctcct tgaagtcggc cagcggaatc tccttgtact gggtctcctg cagcgtcttc
     7081 gcggcggcgt cgaagttggc gagcatggcg tcgttgactt cggcccaggc ggcgatgatc
     7141 ttctcgagca ccggcttgtg gtcatcgaag tagtcgttgc gcgcggccca gccgtcgacg
     7201 atcgccgcct tcgggaagaa cttcgatgcg tcggagatct ttcgcgcccc cggcacctgc
     7261 ttgcgcaccg tctggtcgaa cggcacccac agggccaccg cgggcaccgc gcccgagacg
     7321 aacgaggtca ccgcctcggc catgcgctgg ttgacgatgt gcacgtcctt gttcgggtcg
     7381 agcccggcat cgcgcaatgc gaggtcgaga aagacatgcg cggtggtgcc tgtcgtcgtc
     7441 gagatctgct tgcccttcag gtcggcgacg gtcttgatcg acgggtcgcg cacccagagc
     7501 tgcgccgtcg cgtactcgat gtcgttgatg aggaagacct tgccctggcc gcgggccggg
     7561 aagttggaga cgacggcgcc ggtcgagagc acgtcgaggc tgccgccgat catcgcctgg
     7621 aagagttcga ggccggtcgt gaacttgatc atctcgaggt cgagcccgtg ccgggcccac
     7681 gtgcccgagt gctcgccgat ccagatctgg ccgtcgacgg ccggagtgtg cagatagccg
     7741 acgcgcacct tcgtcttcgc ctgcgcgaag gcggcgagcg acggggtggc gaggctggcg
     7801 gcggtggcct gcaagagggc tctgcggcga atggtcatgg tgttgtctcc ttggctgcgg
     7861 gggatacggg ttgcacggca gtgcgggtct ttcaatcgac gacgcgcacg gcctgccgcg
     7921 cctgcgcttc gtattcctcc atcacgaggg aacggatccg ggcgcgcagc gccgcgaagc
     7981 gcgggtcctc gtgcaccttc tccgaacgcg gatagccgaa cggcacctcg atgacggtgc
     8041 gaacgcgcgc cggccgcgcc gtcacgacga cgatgcgcga agagaggtag atcgcctcgt
     8101 cgaccgagtg cgtgatgagc agcaccgtct tgccttcggc ggcgagcacc tgaagcagca
     8161 ggtcctgcat cgccgagcgc gtctgcgcgt cgagggcgcc gaacggctcg tccat
//
LOCUS       DASRWS010000323         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2420694, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000323 DASRWS010000000
VERSION     DASRWS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420694"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFF72_06080"
     CDS             complement(<1..698)
                     /locus_tag="VFF72_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZV92766.1"
                     /translation="MSRFAFSQVDVFTERPLLGNPLAVVHAADRLDGEQMQAFARWTN
                     LSETAFLLAPTDASADYRVRIFTPGGELPFAGHPTLGSCHAWLEHGGKPGRGSEVVQE
                     CGVGKVRIRIDANGAAFAAPPMRQADVEAGLLERIVAALGLPRSAVRASRSLDNGPTW
                     IGLLLDDAQTVLAIEPDHAALRGLAKVGVIAAQAAGADTDFEVRAFAAPVGVPEDPVT
                     GSLNASLAQWLIETG"
     gene            complement(695..1843)
                     /locus_tag="VFF72_06085"
     CDS             complement(695..1843)
                     /locus_tag="VFF72_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZV92767.1"
                     /translation="MNPSVIREILKLSERPGIISLAGGLPAAETFPVEAMRVAAERVL
                     RESPREALQYAASEGYTPLREWVAEELGTQGFHVDAGNVLITTGSQQGLDLVAKALVD
                     AGSRVAVESPTYLGALQAFTPCEPEFASVECDAEGPMPEALARDAASARFLYVLPNFQ
                     NPAGRSIGAARRAAIARTAEAIGLPLVEDNPYGELWFDAPPPVPIAAHWPAGSVYLGS
                     FSKVLAPGLRLGYIVAPEPVFPKLLQAKQAADLHTPGFNQRIVHEVIRDGFLREHVPT
                     IRARYKAQRDAMQSALTTHLPTTGPRACRWTLPGGGMFFWVELPDDVDAEALLPRAVE
                     RGVAFVPGAPFYAAAPKRNTLRLSFVTVAPPAIERGVAMLAEALESMR"
     gene            complement(2022..2954)
                     /locus_tag="VFF72_06090"
     CDS             complement(2022..2954)
                     /locus_tag="VFF72_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZV92768.1"
                     /translation="MSDAPSSLRRRESRGLMLGFVGVVIFAMTLPMTRLAVGSSAAPQ
                     LPPLFVTAGRAAFAAGLAAAYLVVVRASWPRREHRQLFFVSALGTVVGFPLFLSLALR
                     HVDAMHAAVVTGVLPLATAIAAAVAFRQRPSTGFWLCAGIGCAVVIAFAAWRGNGAVT
                     IGDVLLLGSVASAAIGYVAGARLTQDMSAERVICWVLVMSAPLTVPVMLVSWPTRSAS
                     LAAWGGLAYVSVFSMWMGFFAWYRGLALGGAVRVSQVQLVQPFLSILFAVPVLGERLD
                     VVTIGFALAVVASVVVGRSMPVGRTPAPAMAVRS"
     gene            3016..>3785
                     /locus_tag="VFF72_06095"
     CDS             3016..>3785
                     /locus_tag="VFF72_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV92769.1"
                     /translation="MTAPRSSPPSAARRSAASGARNDASLARRSDTTLAAQLAARYAE
                     LIRQRLLGAGARLPSVRAGAERHGVSPATIVAAYDQLLARGLVEARPQRGFFVRPGAD
                     ATRRSARAVATPARHPLPDSATALIRGMFQAPGRPMPGLGTLPAEWLDAPMLSMAMRR
                     VTSPAQTAATALHYGDPAGEPRLREVLAIGLAEHGIATGSERIVTTVGATNALDIVTR
                     TLLRAGDTVLVDEPGWSVEYARLAALGMRVLPVPRGADG"
BASE COUNT          595 a         1364 c         1369 g          457 t
ORIGIN      
        1 cccgtctcga tcagccattg cgccaggctg gcgttcaggc tgccggtcac cgggtcttcc
       61 ggcacgccga ccggcgcggc gaacgcgcgc acctcgaagt cggtatcggc accagcggcc
      121 tgcgccgcga tgacgccgac cttggccagc ccgcgcaaag ccgcgtggtc gggctcgatc
      181 gccaggacag tctgcgcgtc gtcgagcagc aggccgatcc aggtcgggcc gttgtcgagc
      241 gaccggctcg cccggacggc gctgcgcggc aggccgagcg cggcaacgat gcgctcgagc
      301 aggccggcct cgacgtcggc ctggcgcatc ggcggcgccg cgaacgccgc gccattcgca
      361 tcgatgcgga ttcgcacctt gccgacgccg cattcctgca cgacctcgct gccgcgaccc
      421 ggcttgccgc cgtgctcgag ccaggcgtgg cagctgccga gcgtcggatg gccggcaaac
      481 ggaagctcgc ctccgggcgt gaagatgcgc acacgatagt cggccgatgc atcggtgggt
      541 gcgagcagaa acgccgtctc gctcaggttc gtccagcgcg cgaaggcctg catttgctcg
      601 ccgtccaggc gatcggcggc gtggacgacc gccagcggat tgccgagcag cggccgctcg
      661 gtgaagacgt cgacctggct gaaggcgaat cggctcatcg catggactcg agcgcctcgg
      721 ccagcatcgc gacaccgcgc tcgatcgccg gcggcgcgac cgtgacgaac gaaagccgaa
      781 gcgtgttcct cttcggtgcg gccgcgtaga aaggcgcgcc cggaacgaat gcgacaccgc
      841 gctcgacggc gcgcggcagc agcgcttcgg cgtcgacgtc gtcgggcagc tcgacccaga
      901 agaacatgcc gccgcccggc agcgtccagc ggcaggcgcg cggcccggtt gtgggcaaat
      961 gggtcgtcaa cgccgactgc atcgcgtcgc gctgcgcctt gtagcgggcg cggatcgtcg
     1021 gcacgtgctc gcgcaggaac ccgtcgcgga tgacctcgtg gacgatccgc tggttgaagc
     1081 cgggcgtgtg caggtcggcc gcctgcttgg cctgcagcag cttgggaaag accggttccg
     1141 gagcgacgat gtaacccagc cggagccccg gcgccagcac cttcgagaag ctgccgagat
     1201 agaccgagcc ggccggccag tgcgcggcga tgggcaccgg cggcggcgca tcgaaccaga
     1261 gctcgccgta cgggttgtcc tcgacgagcg gcaggccgat cgcctcggcg gtgcgggcga
     1321 tcgccgcccg tcgcgccgcg ccgatcgagc ggccggcggg gttctggaag ttgggcagca
     1381 cgtagagaaa gcgcgccgac gccgcgtcgc gcgccagtgc ctcgggcatg ggcccttcgg
     1441 cgtcgcattc gacgctcgcg aactcgggct cgcagggcgt gaacgcctgc agcgcgccga
     1501 gatacgtggg cgattcgacg gcgacgcggc tgccggcgtc gaccagcgcc ttggcgacga
     1561 gatcgaggcc ctgctgcgag cccgtggtga tcaggacgtt gccggcgtcg acgtgaaagc
     1621 cctgcgtccc aagctcttcg gcaacccact cgcgcaatgg cgtgtagccc tcgctcgcgg
     1681 catattgcag tgcctcgcgc ggcgactcgc gcagcacgcg ctcggcggcg acccgcatcg
     1741 cctcgaccgg aaaagtctcg gcagcgggca ggccgcccgc cagcgagatg atccccggcc
     1801 gctcgctcag cttgaggatt tcgcggatca ccgacggatt catgcgcccg gttcgccgcg
     1861 cgagacgcca gggcgatggc agcgactgcg cgttcaccac ggcgagctcg ccacgaccac
     1921 gtcgctggcc ggcaacgccg ggcggacttg cagggacgac cacgccaggg cactcctgtg
     1981 cggccgcgcc agggcactcc tgtgcggccg cgcccgagcg ctcatgaccg caccgccatc
     2041 gccggcgccg gtgttcggcc gaccggcatc gaccggccga cgacgacgct ggcgacgacc
     2101 gcgagggcaa agccgatcgt gacgacgtcg agacgctcgc cgagcaccgg cacggcgaac
     2161 aggatcgaca gaaatggctg gaccagctgg acctgactga cccgcactgc gccgccgagc
     2221 gccaggcctc gataccaggc gaagaagccc atccacatcg agaagacgct cacgtaggcg
     2281 aggccgcccc aggcggcgag gctggcggag cgggtcggcc atgacacgag catcaccggc
     2341 accgtcagcg gcgcgctcat gaccaaaacc cagcagatga cccgctcggc cgacatgtcc
     2401 tgcgtgagcc gtgcgcccgc gacatagccg atcgccgcgc tggcgaccga accgagcaac
     2461 aggacatcgc cgatggtgac ggcgccgttg ccccgccacg ccgcgaaggc gatgacgact
     2521 gcgcagccga tcccggcgca gagccagaag ccggtcgacg gccgctgccg gaaggcgacc
     2581 gccgccgcga tcgccgtcgc cagcggcagc acgccggtga ccacggcggc gtgcatcgca
     2641 tcgacgtgac gcaacgccag cgacaggaag agcgggaagc cgaccaccgt gccgagcgcc
     2701 gagacgaaga agagctgtcg atgttcgcgg cgcggccacg acgcccgcac gacgacgagg
     2761 taagccgctg cgagacccgc cgcgaaagcg gcgcgaccgg ccgtgacgaa gagcggcggc
     2821 agttgtggcg cggcgctgct gccgacggcc agccgcgtca tcggcaaggt catggcgaag
     2881 atgacgacgc cgacgaagcc gagcatcagg ccccgcgatt cgcggcgacg aagggatgag
     2941 ggcgcgtcgg acatgagagg gcctttgaca tcgagtgtag gcgagcgagc agcacacgac
     3001 cagtacactt cggccatgac agcgccgcgc tcgtcgcccc cgtccgccgc ccggcgcagc
     3061 gcggcgagcg gcgcgcgcaa cgacgcttcg ctggcgcgcc gttccgacac gacgctcgcc
     3121 gcgcagctgg cggcgcgcta cgccgagctg attcgccagc ggctgctcgg cgccggcgcg
     3181 cggctgcctt cggtgcgggc cggcgccgag cgacatggcg tgagcccggc caccatcgtc
     3241 gcggcctacg atcagttgct ggcacgcggt ctggtcgagg cgcgaccgca acgcgggttc
     3301 ttcgttcgtc ccggcgccga cgcgacccgc cgatcggcgc gagccgtcgc cacgccggcg
     3361 cgccatccgc ttcccgacag cgcgacggca ttgatccgcg gcatgttcca ggcaccgggc
     3421 cggccgatgc cgggcctggg cacgctgccc gccgaatggc tggacgcgcc gatgctgtcg
     3481 atggcgatgc gccgcgtcac gtcgccggca cagacggccg caacggccct gcactacggc
     3541 gatccggccg gcgagccgcg gctgcgcgag gtgctggcga tcggccttgc cgagcacggc
     3601 atcgccaccg gcagcgagcg catcgtcacg acggtcggcg cgacgaacgc gctcgacatc
     3661 gtcacccgga cgttgctgcg cgccggcgac acggtgctgg tcgacgagcc gggctggtcg
     3721 gtcgagtacg cgcggctggc cgcgctcggg atgcgcgtcc tgccggtgcc gcgaggcgcc
     3781 gacgg
//
LOCUS       DASRWS010000324         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4163867, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000324 DASRWS010000000
VERSION     DASRWS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163867"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFF72_06100"
     CDS             <1..587
                     /locus_tag="VFF72_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZV92770.1"
                     /translation="GSVAGVPPLFTDFTYDSVGIPRNWQIAANQAAGGLGYAAKNGIA
                     LGGEYAYYDMGLCGPQRTDLATRASLCGAFKVPTLRNVAIKQNYFHNGVFDNLNDVVS
                     WYVTRDTAPGRWYLKADGSVDIAYNDLPPKFDSGINIAEAPYNPGLAPMLTGDEINLI
                     VVFLCTLTDGYDAGNPQAYGTQAQCLQANAAASH"
     gene            611..2125
                     /locus_tag="VFF72_06105"
     CDS             611..2125
                     /locus_tag="VFF72_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZV92771.1"
                     /translation="MNRFALHPVARAAALVCAAGSAVAAIPAFAAEPQGSNAAVIRKL
                     ENQNETLARTLAEVQQQLQAMKQQLDDMKRQGSSVAEQQKAQGQQVEQAVAQQQRMKT
                     EVAAATQAASQASQQAAVQAARSDVWNRFSLWGYGEVYYLRPTHRSEQTNLDLARAVF
                     GIGYQFDDKTRFNSEYEVEHAVSSADDPGEFEVEQFYVDRKINDAVSGRAGLFLIPAG
                     LLNENHEPTNFYGVQRNFVETLIIPSTWREGGLSVYGTTDSGFDWNVGLTTGMDLSKW
                     DFAPEAPLYNTAQQLINNGVAPLQATHQELALADARRLAQYASLNWRGVPGLTLGGTY
                     FTGGAVPAQPDIGSQRTRLWETHARWQPGRWDLSALYARGSISNTGAANARFPGTANP
                     MPSKFDGWFVQAAYNLWQRDGYRLAPFARWERYDMGAGYEGIAPGFPTTPTGPVPSST
                     SPTTFDYFAVPRDHVATLGANFYLNPNVVLKFDFQKFRTNTDFTRVDLGLGLQF"
     gene            2152..2715
                     /locus_tag="VFF72_06110"
     CDS             2152..2715
                     /locus_tag="VFF72_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZV92772.1"
                     /translation="MLPRSPASRACFVCAAAVPALVSASAFATTYLSAAQAEQALFPD
                     ATAFVAKPLALTPEQSRAIAAQAQTPLQTAFWKVFAATSNGRVLGYVITDSVIGKFDL
                     IDYAVGVAPDGTIRGVEILAYREAHGGEVRTPAWRSQFVGKNAKAPLSVGVDIANISG
                     ATLSCTHLTDGIRRIADYATRVLAAGG"
     gene            2722..3615
                     /locus_tag="VFF72_06115"
     CDS             2722..3615
                     /locus_tag="VFF72_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZV92773.1"
                     /translation="MPVSIAAPRFALRRARPALGTLVDIRVEAASDERAREAMAAAFA
                     EIDAVHRLMSFHDGGSDLSRLNRAVPGERIRVDVRTAEVLGVAEQLRLDSHGAFDCAV
                     ADTLVAQGLLPRPSIPGPHDRADGASIDRAGEDAIGAAVTRGDERLDLGGIAKGFAVD
                     RAIDALRQLGASAGVVDAGGDLRHFGSVPSRVHLRDPADPSSIVATLELRDAALASSA
                     TSGLAASAPSHPRRPSALVDPRDRRRAKPLAPGAGASVMAATCVLADALTKVVLVTGD
                     PRHSLLARHRATLVLHRPASP"
     gene            3612..4109
                     /locus_tag="VFF72_06120"
     CDS             3612..4109
                     /locus_tag="VFF72_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92774.1"
                     /translation="MTRRGRIAVLQLSTRLGGPRRRGLYALVAVLVATGAAWLWLHDM
                     RPADSLPSPIEPWMMKLHGAAALLAVYLLGTMLHGHMLNAWHRRQNRISGGVAAAAVL
                     LLAASGYGLYYFGGDALRSATEWLHWVAGFALPPLLWWHIRSGRLAIGGHASSGRARW
                     RRRER"
BASE COUNT          711 a         1670 c         1460 g          674 t
ORIGIN      
        1 cgggcagcgt cgccggggta cctcccctct tcaccgactt cacctacgac agcgtcggca
       61 ttccgcgcaa ctggcagatc gccgccaacc aggccgccgg cggcctcggc tacgcggcga
      121 agaacggcat cgcgctcggc ggcgaatacg cgtactacga catggggctg tgcgggccgc
      181 agcgcaccga cctggcgacc cgcgcgtcgc tgtgcggtgc cttcaaggtg ccgaccttgc
      241 gcaacgtcgc gatcaagcag aactacttcc acaacggcgt cttcgacaac ctgaacgacg
      301 tcgtcagctg gtacgtcacg cgcgacaccg cgcccgggcg ctggtatctc aaggccgatg
      361 gcagcgtcga catcgcctac aacgacctgc cgccgaagtt cgacagcggc atcaacatcg
      421 ccgaggcccc ctacaacccg ggcctcgcgc cgatgctcac gggcgatgag atcaacctga
      481 tcgtcgtctt tctctgcacc ctcaccgacg gctacgacgc cggcaacccg caggcctacg
      541 gcacgcaggc gcaatgcctg caggccaacg ccgcagcctc ccattgacgc atgcctcccg
      601 gagtccacgc atgaaccgat tcgccctgca tcccgtcgcc cgtgcggccg cgcttgtctg
      661 cgccgcggga agcgccgtcg ccgccattcc ggccttcgcc gccgagccgc aaggcagcaa
      721 tgccgccgtc atcaggaagc tcgaaaacca gaacgagacg ctcgcacgca cgctggccga
      781 ggtgcagcaa caactgcagg ccatgaagca gcagctcgac gacatgaagc ggcagggcag
      841 cagcgtcgcc gagcagcaga aggcgcaagg gcaacaggtc gaacaggcgg tagcgcaaca
      901 gcagcggatg aagaccgagg tcgccgccgc gacccaggcg gcctcgcagg cgagccagca
      961 ggcggcggtg caggccgcga ggtccgacgt ctggaaccgc ttttcactct ggggctacgg
     1021 cgaggtctac tacctgcgtc cgacgcatcg atccgagcag acgaacctcg atctggcgcg
     1081 cgccgtcttc ggcatcggct accagttcga cgacaagacc cggttcaatt ccgaatacga
     1141 ggtcgagcac gccgtgagct cggccgacga tcccggcgag ttcgaggtcg aacagttcta
     1201 cgtcgaccgc aagatcaacg atgccgtgtc cggtcgtgcc gggctcttcc tgattcccgc
     1261 cggcctgctc aacgagaacc acgagccgac caacttctac ggcgtccagc gcaacttcgt
     1321 cgagaccctc atcatcccca gcacctggcg cgaaggcggc ctgtccgtct acggcacgac
     1381 cgattccgga ttcgactgga acgtcggcct gacgaccggc atggatctct cgaaatggga
     1441 tttcgcgccg gaagcgccgc tctacaacac ggcccagcaa ctgatcaaca acggcgtcgc
     1501 gccgctgcag gccacgcatc aggagctcgc cctggccgat gccaggcggc tggcgcagta
     1561 cgcctcgctc aactggcgcg gcgttcccgg cctgaccctc ggcggcacct atttcacggg
     1621 cggcgccgtg ccggcgcagc ccgacatcgg cagccagcgg acccgactgt gggagacgca
     1681 cgcgcgctgg cagccgggcc gctgggacct ctcggcgctc tacgcccgtg gctccatcag
     1741 caacaccggc gctgccaacg ccaggtttcc cgggacggcc aatccgatgc cctcgaagtt
     1801 cgacggctgg ttcgtgcagg ccgcctacaa cctctggcag cgcgacggct accggctggc
     1861 gcccttcgca cgctgggagc gctacgacat gggcgccggc tacgagggga tcgccccggg
     1921 gtttccgacc accccgaccg gcccggtacc cagctcgaca tcgcccacga ccttcgacta
     1981 cttcgccgtt ccacgcgacc atgtcgcgac gctgggcgcg aacttctatc tcaatcccaa
     2041 cgtcgtgctg aaattcgact tccagaagtt caggaccaac accgacttca cgcgcgtcga
     2101 tctggggctc ggcctgcagt tctgatcgtc gcgctcgaag accgcattcc catgctgccg
     2161 cgatctcccg cctcccgggc ctgcttcgtc tgtgccgccg ccgtgccggc gctcgtctcg
     2221 gcatcggcct tcgcgaccac gtacctgagc gccgcccagg ccgagcaggc gctctttccg
     2281 gacgccacgg cgttcgttgc gaagccgttg gccctgacgc ccgagcagtc gcgtgccatc
     2341 gcggcacagg cgcagacacc gctgcagacg gcattctgga aggtcttcgc ggcgacgtcg
     2401 aacggacgcg tgctgggtta cgtgatcacc gacagcgtca tcggcaagtt cgatctcatc
     2461 gactacgccg tcggcgtggc gcccgatggc acgatccgcg gcgtcgagat cctcgcctat
     2521 cgcgaagccc acggcggcga ggtccggacg ccggcgtggc ggagccagtt cgtcggcaag
     2581 aacgcgaagg caccgctgtc ggtcggcgtc gacatcgcca acatcagcgg cgcgaccctg
     2641 tcgtgcacgc acctcaccga tggcatccgg cggatcgcgg actacgcgac gcgggtgctc
     2701 gcggccggag gttgaggagc gatgccggtc tcgatcgccg cgccacgctt cgccttgcgc
     2761 cgggcccggc cggcactcgg cacgctggtc gacattcgcg tcgaggcggc cagcgacgag
     2821 cgtgcccgcg aggcgatggc ggccgccttc gccgagatcg acgccgtgca ccgactgatg
     2881 agctttcacg atggcggcag cgacctgtcc cggctcaacc gggccgtgcc cggcgagcgg
     2941 attcgtgtcg acgtgcgcac cgccgaagtg cttggcgttg ccgaacagct gcggctcgat
     3001 tcgcacggcg ccttcgattg cgccgtcgcc gacacgctgg tcgcgcaagg cttgctgccg
     3061 cggccttcga tcccggggcc gcacgaccgc gccgatggtg catcgattga ccgtgccggc
     3121 gaggacgcca tcggcgcggc ggtgacacgc ggcgacgagc ggctcgacct cggcggcatt
     3181 gccaagggct tcgccgtcga ccgcgccatc gacgcgctgc gccaactcgg cgcgagcgcc
     3241 ggtgtcgtcg acgccggtgg cgacctgcgc catttcggca gcgtgccgtc gcgcgtccat
     3301 ctccgcgatc cggccgatcc gtcatccatc gtcgccacgc tcgagctgcg cgatgccgcg
     3361 ctcgcgagct cggcgacgag cggcctcgcg gcgtcggcgc catcgcaccc acggcgtccg
     3421 tcggcgctgg tcgaccctcg cgaccgtcgc cgggcgaaac ctctcgcgcc cggcgccggc
     3481 gccagtgtca tggcggccac ctgcgtgctc gccgacgcac tgacgaaggt cgtcctcgtc
     3541 accggcgacc ccaggcattc gctcctcgcc aggcatcggg ccacgctcgt gctgcaccga
     3601 ccggcgtcgc catgacgcga cgcggccgga tcgccgtact gcaactctcg acgcgcctcg
     3661 gcggcccgcg ccggcgcggc ctctacgccc tcgtcgccgt tctcgtcgcg acgggtgccg
     3721 cgtggctatg gctgcacgac atgcgcccgg ccgactcgct gccgagcccg atcgagccct
     3781 ggatgatgaa gctgcacggc gccgccgcac tgctcgcggt ctaccttctc ggcaccatgc
     3841 tccatggcca catgctcaac gcctggcatc gaaggcagaa ccgcatcagc ggcggtgtcg
     3901 cggctgcagc cgtgctgttg ctggcggcga gcggctacgg tctctactat ttcggcggcg
     3961 acgcgctcag aagcgcgacg gagtggctgc actgggtggc gggcttcgcg ctgccgccgc
     4021 ttctgtggtg gcacatccgc agcggtcgcc tcgcgatcgg cggtcacgcc tcttcggggc
     4081 gagcgcggtg gcgtcgccga gagcgatgag gcgacgtcga tcgcgcagta ggaaccgcgc
     4141 atcgactttc gttcatccgc gagaacgccc atcccgccag ccggacactc ctcgctagac
     4201 tccgggcgac ttgcccggcg ccgggtgccc atcgacaacc cccctcatga gtcaggacac
     4261 gccccctgcc ggcaacgccg gcgacaagga catcgacaag gatgccgcaa ggcggatgcg
     4321 cgagcgcgtg aagcgctcgt tgcccggccc gcacgcacga gcagcggctc gttgcgcacc
     4381 gctggcggcg tgctcgagta cgtcgtctcg gccgccttca tgcccgtcgt cgccgaaggc
     4441 ttcgacggcg cgctgggcga ccctcaggca gcggtgatgg cgacgagcta tgttcttgcc
     4501 ggcagcgatc cggcg
//
LOCUS       DASRWS010000325         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_132932, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000325 DASRWS010000000
VERSION     DASRWS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132932"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFF72_06125"
     CDS             <1..468
                     /locus_tag="VFF72_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92775.1"
                     /translation="EGAPIMVQARSLGIEVPFIGGNGMNSVKVFDLAKGKSDNLWVGS
                     PWSIDNDAKANQAFVAAYKDKYKNPPDQFAAQAFDAVHIAAVALRKIKLSGNLDADRK
                     ALRDALPSVTWDGATGPFKFRQAADSSGKPAGYDAHQAAIVSVTKDGKYLIEK"
     gene            484..1365
                     /locus_tag="VFF72_06130"
     CDS             484..1365
                     /locus_tag="VFF72_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92776.1"
                     /translation="MFDQQLVNALSLGCVYALFALGFTLVFGVLGVINLSHGAVFMVG
                     AYAALTVVTRLAAPLWVALLAAIVTSGLLGLAIDVLVLRPLRRRGAPHLVPMIATIGV
                     AIFFNSAMQGLFGAENQRFPPDLIPDTVLHFAGVQVSVLEVAIIVLSFVLMAALLFVM
                     RRTRLGRALRAIAESPRAAALLGINVQGLFYLTSFSAAALGGVAGVLIALYSNAVFPL
                     MGQPMLEKGIAVIILGGMGDIRGALVGSLFLGFAEVMSVAYVGSTVRDAVAFGLLFIV
                     LLVRPQGLFGRTLERKA"
     gene            1397..2287
                     /locus_tag="VFF72_06135"
     CDS             1397..2287
                     /locus_tag="VFF72_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92777.1"
                     /translation="MDTLDTLWAVYGNLVLTIGINALLALSIWLTLSCGLLAMANAAF
                     MGLGAYAGALLTMRAGVPFPVALAGGMALPALVALVIGLPTLRLSGVYLAMATLGFGE
                     VVRIGLINADSLTGGALGLNAIPQLTRWWHVVLALVVVLLVLWRMRRSRVGRAFEAIR
                     EDETAASLMGIGVMSHKMLAFCLGAAVAGLAGVLGAHLTFFIGPGEFGFERSVDILTM
                     AILGGIGSLAGPVLGAAIITLLPELLRAFHDFRTMLNGAILVLIVLFLPNGLWDPARI
                     RSWFVRRRPSGAAATPAGRP"
     gene            2299..3054
                     /locus_tag="VFF72_06140"
     CDS             2299..3054
                     /locus_tag="VFF72_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92778.1"
                     /translation="MLALDRVSKRFGGLAVLEDVSFAVPAGSVFGLIGPNGAGKTTVF
                     NLVTGLLAPSSGSITLNDQALVGRRPHRITRLGVARTFQNIRVFKGLTLRDNVIVGMH
                     SHLGYGVPQWLFSLPAFRAREARAVERAEELLSWVGLPGRGDAVAQDLSYGDQRKLEL
                     ARALATEPRLLLLDEPVAGMNSGEKQELMVEIGNIAARGYTILLIEHDMRFVMGLCSR
                     IAVLNFGRIIAEGSPETIRSDPAVIEAYLGRDE"
     gene            3051..3773
                     /locus_tag="VFF72_06145"
     CDS             3051..3773
                     /locus_tag="VFF72_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92779.1"
                     /translation="MTRGQALLEVSGLVVSYGHIEAVRGVDLRVDTGEIRTLVGANGA
                     GKSTTLLALSGLVAPRAGTVRFAGDDITGMAPHRIVERGLVQVAEGRAILTTMSVREN
                     LELGAYRRHDTAAVASDMERVLDLFPRLRERLGGFAGNLSGGEQQMLAIARALLARPR
                     MLLLDEPSMGLAPIAVQGILDALRKIHEQGITVFLVEQNVRQALKLASHGYVIETGRI
                     VLEGPSAQLMDDPRVVAAYLGG"
     gene            complement(3801..4142)
                     /locus_tag="VFF72_06150"
     CDS             complement(3801..4142)
                     /locus_tag="VFF72_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HZV92780.1"
                     /translation="MTLQTQRLDADVCVAGQLETAAMAEAAAAGFRSVINNRPDFEGG
                     PGQPTSEAMAAAAAAAGLSYAFLPVAGGYQSPEEIARFAQLLASMPKPILAFCRSGAR
                     SGKLWQAATAG"
     gene            complement(4154..>4408)
                     /locus_tag="VFF72_06155"
     CDS             complement(4154..>4408)
                     /locus_tag="VFF72_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZV92781.1"
                     /translation="TQFAAGARQDLVDKETAELQILETYLPQRLSADAVGIEVAAVVA
                     EIGASGPADMGRTMAAAKARLGGRADMATVSAAVKAALSR"
BASE COUNT          589 a         1552 c         1493 g          774 t
ORIGIN      
        1 gaaggcgcgc cgatcatggt gcaggcacgc agcctcggca tcgaggtgcc gttcatcggc
       61 ggcaacggca tgaattcggt gaaggtgttc gacctcgcca agggcaagag cgacaacctc
      121 tgggtcggca gcccgtggtc catcgacaac gacgcgaagg ccaaccaggc cttcgtcgcc
      181 gcctacaagg acaagtacaa gaacccgccc gaccagttcg ccgcccaggc cttcgatgcc
      241 gtccacatcg ccgccgtcgc gctgcgcaag atcaagctga gcggcaatct cgacgctgac
      301 cgcaaggcgc tgcgcgacgc gctgccgtcg gtcacctggg acggcgcgac cggccccttc
      361 aagttccggc aggccgccga cagcagcggc aagccggccg gctacgacgc ccatcaggcg
      421 gcgatcgtga gcgtcaccaa ggacgggaag tacctgatcg agaagtaggc tgctgccgcg
      481 gcgatgttcg atcagcagct cgtcaacgcg ctatcgctcg gctgcgtcta tgcgctgttc
      541 gcgctcgggt tcacgctcgt cttcggcgtg ctcggcgtca tcaacctttc gcacggcgcg
      601 gtcttcatgg tgggcgccta cgcggcgctc accgtcgtca cgcggctggc ggcgccgctc
      661 tgggtggcgc tgctcgccgc gatcgtgacg agcggcctgc tcgggctcgc catcgacgtc
      721 ctcgtgctgc ggcccctgcg ccgtcgcggc gcgccgcacc tggtgccgat gatcgcgacg
      781 atcggcgtcg cgatcttctt caacagcgcc atgcagggcc tgttcggtgc cgagaaccag
      841 cgctttccgc ccgatctgat ccccgacacc gtgcttcact tcgccggcgt ccaggtcagc
      901 gtgctggaag tcgcgatcat cgtcctgtcg ttcgtgctga tggcggcgct cctgttcgtg
      961 atgcgccgga cccggctcgg ccgggccttg cgcgccatcg ccgaatcgcc gagggcggcg
     1021 gcgctccttg gaatcaatgt ccagggcctt ttctatctca cgtcgttcag cgcggcggcg
     1081 ctcggcggcg tcgccggcgt gctgatcgcg ctctactcga acgcggtttt cccgctcatg
     1141 ggccagccga tgctggaaaa gggcatcgcc gtcatcatcc tcggcggcat gggcgacatc
     1201 cgcggcgcgc tcgtcggcag tctcttcctc ggcttcgccg aagtgatgtc ggtcgcctac
     1261 gtcggctcga cggtgcgcga cgcggtcgcc ttcggcctgt tgttcatcgt ccttctcgtc
     1321 cggccgcagg gccttttcgg tcgcaccctg gaacgcaagg cgtgaggccg gccgaccgtt
     1381 ggcttcgccg gcattgatgg acaccctgga tacgctctgg gccgtctacg gcaacctggt
     1441 gctgacgatc ggcatcaacg ccttgctcgc gctgtcgatc tggctcacgc tgtcctgcgg
     1501 cctcctggcg atggccaacg cggcgttcat gggccttggc gcctacgccg gcgccctgct
     1561 cacgatgcgt gccggcgtgc cctttccggt cgcgctcgcc gggggcatgg cgctgccggc
     1621 gctggtggcg ctcgtgatcg gcttgccgac attgcggctg tctggggtct atctggcaat
     1681 ggccacgctc ggcttcggcg aggtcgtgcg catcggcctg atcaacgccg actcgctcac
     1741 cggcggcgcc ctcggcctca acgcgatccc gcagctcacc cgttggtggc acgtcgtcct
     1801 cgccctggtc gtcgtcctgc tcgtcctctg gcggatgcgc cgctcgcggg tcggtcgtgc
     1861 cttcgaggcg atccgcgaag acgagactgc ggcgtcgctg atgggcatcg gcgtgatgtc
     1921 gcacaagatg ctcgcgttct gtctcggcgc cgcggttgcc ggtctggccg gggtgctcgg
     1981 cgcccacctg acgttcttca tcggcccggg cgagttcggc ttcgagcgca gcgtcgacat
     2041 cctgacgatg gccatcctcg gcggcatcgg cagcctcgcc gggccggttc tcggcgccgc
     2101 gatcatcacg ctgctgccgg agttgctgcg cgccttccac gacttccgta ccatgctcaa
     2161 cggcgcgatc ctcgtcctca tcgtcctctt cctgcccaac ggcctgtggg atccggcgcg
     2221 gatccgatcc tggttcgtcc ggcgccggcc ttccggtgct gccgcgacgc cggccggccg
     2281 tccctgaacc ggcgcagcat gctcgctctc gatcgcgtct cgaagcgctt cggcggcctc
     2341 gctgtcctcg aggacgtgag cttcgccgtc ccggccggca gcgtcttcgg cctcatcggg
     2401 ccgaacggcg ccggcaagac gaccgttttc aatctcgtca cggggttgct tgcgccgagc
     2461 agcggatcga tcaccctgaa cgaccaggcg ctggtcgggc gccgtccgca ccggatcacg
     2521 cgactcggtg tggcgcgcac cttccagaac atccgcgtct ttaaaggact gacgctgcgc
     2581 gacaacgtga tcgtcggcat gcattcgcac ctcggctacg gcgtgccgca gtggctgttt
     2641 tccctgccgg cgttccgggc tcgcgaggcg cgcgcggtcg aacgcgccga ggagctgctg
     2701 tcgtgggtcg gcttgcccgg acgaggcgat gcggtggcgc aggacctctc gtacggcgat
     2761 caacgcaagc tcgagctggc ccgggcgctg gccaccgagc cccgcctgct gctcctcgac
     2821 gagcccgtcg ccggcatgaa cagcggcgag aagcaggagc tgatggtcga gatcggcaat
     2881 atcgcggcgc gcggctacac gatcctgctg atcgagcacg acatgcgctt cgtgatgggc
     2941 ctgtgcagcc gtatcgcggt gctcaacttc ggcaggatca tcgccgaggg ctcgccggag
     3001 acgatccgca gcgatccggc ggtgatcgag gcctacctgg gtcgcgacga atgacgcgcg
     3061 gccaagcgct tctcgaagtc tcggggctcg tcgtgtcgta cggccacatc gaggcggtcc
     3121 gcggcgtcga cctgcgcgtc gacaccggag agatccgcac cctggtcggt gccaacggtg
     3181 ccggcaagtc gaccacgctg ctggccttgt cgggcctggt cgcacctcgc gccggaacgg
     3241 ttcgcttcgc cggcgacgac atcaccggga tggcgccgca ccgcatcgtc gagcgcgggc
     3301 tcgtccaggt cgccgaggga cgcgccatcc tgacgacgat gtcggtgcgc gagaacctcg
     3361 agctcggcgc ctatcggcgg cacgacacgg ccgccgtcgc gagcgacatg gagcgcgtgc
     3421 tcgatctctt tccccgtctg cgggagcggc tgggcgggtt cgccggaaac ctttccggcg
     3481 gcgagcagca gatgctcgcc atcgccaggg cgctgctcgc gcggccacgc atgcttcttc
     3541 tcgacgagcc ttcgatgggc ctcgcgccga tcgcggtgca gggcatcctc gatgccttgc
     3601 gcaagatcca tgagcagggc atcaccgtct ttctcgtcga gcagaacgtt cgccaggcac
     3661 tcaagctggc aagccacggc tatgtcatcg agacgggccg catcgttctc gaagggccga
     3721 gcgcgcagct gatggacgat ccgcgcgtgg tggcggcgta tctcggggga tagaggcccc
     3781 gacttcgcgc cgaggagagc tcagccggcg gttgccgcct gccagagctt tcccgagcgg
     3841 gcgccgctgc ggcaaaaggc gaggatcggc ttcggcatcg aggcgagcag ctgggcgaag
     3901 cgggcgatct cctccgggct ctggtacccc ccggccacgg gcaggaaggc atagctgagg
     3961 cccgccgccg ccgccgccgc cgccatcgcc tcgctcgtcg gctggcccgg gccgccttcg
     4021 aaatcgggcc ggttgttgat gacgctgcga aagcccgcgg ccgcagcctc ggccatcgct
     4081 gccgtctcga gctgaccggc gacgcagacg tcggcgtcga ggcgttgtgt ctggagggtc
     4141 atgtggttgg tcctcagcgc gagagcgctg ccttgacggc ggccgagacg gtcgccatgt
     4201 ccgcgcgacc gccgagtcgc gccttcgccg ccgccatcgt ccggcccatg tcggccggcc
     4261 ccgacgcgcc gatctcggcg accacagccg ccacctcgat gccgacggca tcggcgctca
     4321 gacgctgcgg cagataggtc tcgaggattt gcaactcggc ggtctccttg tccacgagat
     4381 cctgccgagc gccggcggcg aactgggt
//
LOCUS       DASRWS010000326         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3012991, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000326 DASRWS010000000
VERSION     DASRWS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3012991"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2279
                     /locus_tag="VFF72_06160"
     CDS             <1..>2279
                     /locus_tag="VFF72_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZV92782.1"
                     /translation="VAEGKDAQGHVAVAAASIWITKQGELWFSQDNDDRIDVLPEKKS
                     YEPGETARFQVRMPFREATALVAVEREGIIATQVVTLRGDDPTVTLKIDPAWSPNVYV
                     SVLALRGRIRETPWYSFFTWGWKEPLSWWHSFWGDSKDYVAPTAMVDLAKPAFKLGVA
                     AIKVGRAAHELQVSVVADKAQYQVRQTARARIRVTQNGKPLAGADVAFAAVDEGLLAL
                     HANESWNLLDAMIQARGWGVETSTAQSEIIGRRHYGKKAVAAGGGGGRGATRELFDTL
                     LVWKPSVALDANGEATVDVPLNDSLTSFRLVAIADADVQKFGTGSTRIRVTQDLQVLA
                     GLPPLVRDGDRFLAMLTLRNTTTHEMKVHASLQGTANLPGANGTDIARLPIALPAQDV
                     TLAAGAAREVSWPVDVPADASSITWEAAAEATGARDRLKTTQLVAAAVPVRVLQATLS
                     QLDGGFSLPVAAPADALPASGPKRGGLLVAVQPKLTGALPGMRRFFETYPFICLEQKT
                     SKSVGLKDATMWQGVANTLPTYLDSDGLASYFPPRDGDPASGSDSLTAYVLAATNEAG
                     FALPAPAQEAMLGGLTAFVEGRIERKFWSPRADLDVRKIAAIEALSRYGRAEPKMLGS
                     VNVTPNVWPTAAVINWLMILKRVDAIPDRARKLDEANQILRSRLTYAGTTLRFSNEDS
                     DFWWWLMDNADANAAKLILAVIDDPAWRDDLPKMVVGSLARQRHGAWLTTTANLWGSL
                     ALDRFAAKFESEKVAGRTM"
BASE COUNT          367 a          786 c          805 g          321 t
ORIGIN      
        1 tcgtcgccga gggcaaggac gcgcagggcc acgtcgcggt ggccgcggcg agcatctgga
       61 tcacgaagca gggcgagctc tggttctccc aggacaacga cgaccgcatc gacgtgctgc
      121 ccgagaagaa gagctacgaa ccgggcgaga ccgcgcgctt ccaggtgcgc atgccgtttc
      181 gcgaggcgac ggcgctggtg gcggtcgagc gcgagggcat catcgcgacg caggtcgtga
      241 cgctgcgcgg cgacgatccg accgtcacgc tgaagatcga cccagcgtgg tcgccgaatg
      301 tctacgtgag cgtcctcgcg ttgcgtggac ggatccgcga gacaccctgg tattcgttct
      361 tcacctgggg ctggaaggag ccgctgtcct ggtggcacag cttctggggc gacagcaagg
      421 actacgtcgc gccgacggcg atggtcgatc tcgccaagcc ggcgttcaag ctcggcgtcg
      481 cggcgatcaa ggtcggccgc gccgcgcacg agctgcaggt gagcgtcgtt gccgacaagg
      541 cgcaatacca ggtgcggcag acggcgcggg cgcggatccg cgttacccag aacggcaagc
      601 cgctggccgg cgccgacgtc gcgttcgccg cggtcgacga gggcctgctc gcgctgcatg
      661 ccaacgagtc gtggaacctg ctcgacgcga tgatccaggc gcgcggctgg ggcgtcgaga
      721 cgagcacggc gcaaagcgag atcatcggcc ggcgccacta cggcaagaag gcggtcgcgg
      781 ccggcggtgg cggcggccgc ggcgccacgc gcgagctctt cgacacgctg ctggtctgga
      841 agccgagcgt cgcgctcgac gccaacggcg aggccaccgt cgacgttccg cttaacgact
      901 cgctgaccag cttcaggctg gtcgcgatcg ccgatgccga cgtgcagaag ttcggcaccg
      961 gcagcacccg catccgcgtc acgcaggatc tgcaagtcct cgcggggctg ccgccgctgg
     1021 tgcgtgacgg cgatcgattc ctggcgatgc tgaccctgcg caacacgacg acgcacgaga
     1081 tgaaggtgca cgcgagcctt cagggcacgg cgaacctgcc cggcgcgaac ggcaccgaca
     1141 tcgcccgcct gccgatcgcc ttgccggcgc aggacgtgac gctcgccgcc ggcgccgccc
     1201 gcgaggtctc gtggcccgtc gacgtgcccg ccgacgcctc cagcatcacc tgggaagccg
     1261 ccgccgaggc gaccggcgcc agggaccgcc tgaagacgac gcagctcgtt gccgcggcgg
     1321 tgccggtgcg cgtgctgcag gcgaccctga gccagctcga cggcggcttc agcctgccgg
     1381 tcgccgcacc ggccgatgcc ttgccggcct cgggcccgaa gcgcggcggt ctgctcgtcg
     1441 ccgtgcagcc gaagctgacg ggcgcgctgc ccggcatgcg tcgcttcttc gagacctacc
     1501 ccttcatctg cctcgagcag aagacctcga aatcggtcgg cctgaaggac gcgacgatgt
     1561 ggcagggcgt cgccaacacc ctgccgacgt atctcgacag cgacggcctg gccagttact
     1621 tcccgccgcg cgacggcgac cccgcgagcg gcagcgacag cctgaccgcg tacgtgctcg
     1681 cggcgacgaa cgaagccggc tttgccctgc cggcgccggc gcaggaggcg atgctcggtg
     1741 gcctgacggc gttcgtcgag ggacggatcg agcggaagtt ctggtcgccg cgtgccgacc
     1801 tcgacgtgcg caagatcgcc gccatcgagg cgctgtcgcg ctacggtcgg gccgaaccga
     1861 agatgctcgg ctcggtcaac gtcacgccta acgtctggcc gacggcggcc gtcatcaact
     1921 ggctgatgat cttgaagcgc gtcgacgcga ttccggatcg tgcccgcaag ctcgacgagg
     1981 ccaaccagat cctgcgctcg cgcctcacct acgccggcac gacgctgcgc ttcagcaatg
     2041 aagacagcga cttctggtgg tggctgatgg acaacgccga cgccaatgcc gccaagctga
     2101 tcctcgccgt gatcgacgat ccggcctggc gcgacgacct gccgaagatg gtcgtcggct
     2161 cgctggcacg gcagcgacat ggcgcgtggc tgacgacgac ggccaatctc tggggctcgc
     2221 tcgctctcga caggttcgcc gcgaagttcg agtcggagaa ggtggcgggc cggacgatg
//
LOCUS       DASRWS010000327         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2712202, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000327 DASRWS010000000
VERSION     DASRWS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2712202"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2147)
                     /gene="acnA"
                     /locus_tag="VFF72_06165"
     CDS             complement(<1..2147)
                     /gene="acnA"
                     /locus_tag="VFF72_06165"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZV92783.1"
                     /translation="MPHAFADTVKTFTTRAGKQGRFWSLPALARQFPNVSRLPVSIRI
                     VLESVLRNCDGKKVLPEHVAELAGWKANAPRVDEIPFVVARVVLQDFTGVPLLADLAA
                     MRNVADEMGKDAKTIEPLVPVDLVVDHSVMIDYFGTKKALELNMQLEFKRNLERYQFM
                     KWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKSPAKKGEAPVYYPDSLVGTDSHTT
                     MINGIGVVGWGVGGIEAEAAMLGQPVYFLTPDVVGFEFTGKLREGVTATDLVLAVTEI
                     LRKEKVVGKFVEFYGDGVAGLSVPDRATMANMAPEYGATMGFFPVDDKTIDYFEGTGR
                     TRDEIEAFRAYFKAQGMYGVPRAGQIDYSHVVTLDLGTVAPSLAGPKRPQDRIELGNV
                     KKQFASLFSKPPTESGFNQPAERLLTRHLVRAHDVRSVEPDGKTPRNPSVKPGSPRFV
                     EEMSANKPTLASALHEAEPAQPALPDLTVGNGDVLIAAITSCTNTSNPSVMLAAGLLA
                     KKAVEAGLKVKPHIKTSLAPGSRIVTEYLTRGGLLPYLEKLGFNLAAYGCTTCIGNAG
                     DLTPELNDAINKNDLVCAAVLSGNRNFEARIHPNIKANFLASPPLVVAYAIAGTVLKD
                     LMTEPVGKGKGGKDVWLGDIWPTSDEIAALMKYAMNGKSFKANYDKVATEPGPLWQKI
                     KGVSGQVYNWPGSTYIARPPFFEGFGMEPPVTDA"
     gene            complement(2177..2584)
                     /locus_tag="VFF72_06170"
     CDS             complement(2177..2584)
                     /locus_tag="VFF72_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZV92784.1"
                     /translation="MIAVDSSVLVDVLVADPRFAPSSEASLGEALSLGDVVICDAVVA
                     ELHTLLAGSETTMESLSTLGIRYLPTSEQASVRAGHMQRRFRDRGGRRDRVVADFLIG
                     AHAMLQCDALVTRDAAFFRDYFKGLKVIEPRPR"
     gene            complement(2581..2865)
                     /locus_tag="VFF72_06175"
     CDS             complement(2581..2865)
                     /locus_tag="VFF72_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZV92785.1"
                     /translation="MEATVAERGQITLPKAVRDALGLAKGTQLKVELDGGRIILRKNV
                     DDALSKLRGRFKLAEGFATTDDAMRALRGRAPGDPYVPPGESAGPARKKK"
     gene            complement(2923..>3422)
                     /gene="serC"
                     /locus_tag="VFF72_06180"
     CDS             complement(2923..>3422)
                     /gene="serC"
                     /locus_tag="VFF72_06180"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZV92786.1"
                     /translation="KNVGPAGLTFVIVRDDLLGGAAKTTPQAFDYTVQAANNSMINTP
                     PTFAIYMSGLVFRWLKEQGGLAEIEKVNRAKAALLYETIDATPFYRNRVAAEDRSIMN
                     VVFHLADPQLDTDFLDGARAAGLLNLKGHRIAGGMRASIYNAMPMQGVQALVSYMKDF
                     AARRG"
BASE COUNT          524 a         1080 c         1196 g          622 t
ORIGIN      
        1 gcgtcggtga cgggcggctc catgccgaag ccctcgaaaa agggcggccg ggcgatgtag
       61 gtcgagcccg gccagttgta gacctggccg ctcacgccct tgatcttctg ccagagcggt
      121 cccggctccg tcgcgacctt gtcgtagttc gccttgaacg acttgccgtt catcgcgtat
      181 ttcatcagcg cggcgatctc gtcgctggtc ggccagatgt cgcccagcca gacgtccttg
      241 ccgcccttgc ccttgccgac cggctcggtc atcaggtcct tgaggacggt gccggcgatg
      301 gcgtaggcga cgacgagcgg cgggctggcc aggaagttgg ccttgatgtt cgggtggatc
      361 cgcgcctcga agttgcggtt gcccgagagc acggccgcgc agacgaggtc gttcttgttg
      421 atcgcgtcgt tgagctccgg cgtgaggtcg ccggcgttgc cgatgcaggt ggtgcagccg
      481 taggcggcga ggttgaagcc gagcttctcg agatacggca gcaggccgcc cctggtgagg
      541 tattcggtga cgatccgcga gcccggcgcg agcgacgtct tgatgtgcgg cttgaccttc
      601 aacccggcct cgaccgcctt cttggcgaga agccccgcag cgagcatgac gctcgggttc
      661 gaggtgttcg tgcacgaggt gatcgcggcg atcagcacgt cgccgttgcc gacggtcagg
      721 tccggcagtg ccggttgcgc gggctccgcc tcgtgcagcg ccgacgccag cgtcggcttg
      781 ttcgccgaca tctcctcgac gaagcggggc gaacccggct tgaccgacgg attcctcggt
      841 gtcttgccgt cgggctcgac gctccgcacg tcgtgggcgc gcacgagatg gcgtgtcagc
      901 agccgttcgg cgggctggtt gaagccgctt tccgtcggcg gcttgctgaa gagcgaggcg
      961 aactgcttct tgacgttgcc gagctcgatg cggtcctggg gccgcttcgg gccggcgagg
     1021 ctcggcgcca ccgtgccgag gtcgagcgtg acgacgtggc tgtagtcgat ctgcccggca
     1081 cgcggcaccc cgtacatgcc ttgcgccttg aagtaggcgc gaaaggcctc gatctcgtcg
     1141 cgcgtccggc cggtgccttc gaaatagtcg atggtcttgt cgtcgaccgg gaaaaagccc
     1201 atcgtcgcgc cgtattcggg cgccatgttg gccatcgtcg cgcggtcggg aaccgacagg
     1261 ccggcaacgc catcgccata gaactcgacg aacttgccga ccaccttctc cttgcgcagg
     1321 atctcggtga ccgccagcac caggtcggtg gccgtgacgc cttcgcgcag cttgccggtg
     1381 aattcgaagc cgacgacgtc gggcgtcagg aaatagaccg gctggccgag catcgccgct
     1441 tcggcctcga tgccgccgac gccccagccg acgacgccga tgccgttgat catggtcgtg
     1501 tggctgtcgg tgccgacgag ggaatcggga tagtagacgg gggcttcgcc cttcttcgcc
     1561 ggcgacttgt ggacgccgcg cgcgaggtac tcgagattga cctggtggac gatgccgaac
     1621 cccggcggca cgacgccgaa ggtgtcgaac gcctgcattc cccatttcat gaactggtag
     1681 cgctcgagat tgcgcttgaa ctcgagctgc atgttgagct cgagcgcctt cttcgtgccg
     1741 aagtagtcga tcatcaccga atggtcgacg acgaggtcga ccggaacgag cggctcgatc
     1801 gtcttggcgt ccttgcccat ctcgtcggcg acgttgcgca tggcggcgag gtcggccagc
     1861 agcggcacgc cggtgaaatc ctgaaggacg acccgggcga cgacgaacgg aatttcatcg
     1921 acgcgcggcg cgttcgcctt ccacccggcc agctcggcga catgctcggg cagcactttc
     1981 ttgccgtcgc agttgcgaag caccgactcg aggacgatgc ggatcgacac cggcaggcgc
     2041 gagacgttcg ggaactgcct ggcgagcgcg ggcaaggacc agaagcgacc ttgcttgccg
     2101 gcgcgggtgg tgaaggtctt gacggtatcg gcaaaggcgt gtggcatggt gttcctcgca
     2161 aggcgtgggt gagggttcag cgtgggcgcg gttcgatgac cttgagcccc ttgaagtaat
     2221 cgcggaagaa agcggcgtcg cgcgtgacga gcgcgtcgca ctgcagcatg gcgtgggcgc
     2281 cgatcaggaa atcggcgacg acgcggtcgc gccggccgcc ccggtcgcga aagcgccgct
     2341 gcatgtggcc tgcgcgcacc gatgcctgct cgctcgtcgg caggtagcgg atgccgagcg
     2401 tggacaggct ttccatggtc gtctcggagc cggccagcaa ggtgtgcagt tcggccacga
     2461 ccgcgtcgca aatgacgacg tcgccgagcg acagcgcctc gcccaggctc gcttcggagg
     2521 aaggcgcgaa ccgcgggtcg gccaccagga cgtcgaccag gacggaagaa tcgacggcga
     2581 tcatttcttc ttcctggccg ggccagcgct ttcgccgggc ggcacgtacg gatcgcccgg
     2641 cgcccgaccg cgcagcgccc gcatcgcgtc gtcggtggtc gcgaatccct cggcgagctt
     2701 gaagcggccg cgcagcttgg aaagggcgtc gtcgacgttc ttgcgcagga tgatgcggcc
     2761 gccgtcgagc tcgaccttga gttgcgttcc cttggccagg ccgagcgcat cgcgcacggc
     2821 cttcggcaac gtgatctggc cgcgctccgc gaccgtcgct tccatactga tgttgtctga
     2881 cgggattggt atgcttgatt tttgcatgct tcgggcgcca gctcaacccc gccgggcggc
     2941 gaaatccttc atgtaggaaa ccagcgcctg cacgccttgc atcggcatgg cgttgtagat
     3001 cgaggcgcgc atcccgccgg cgatccggtg gcccttcagg ttcaggaggc ctgcggcgcg
     3061 ggcgccgtcg aggaaatcgg tgtcgagctg cgggtcggcg aggtggaaga cgacgttcat
     3121 gatcgaacgg tcttcggcgg cgactcggtt acgatagaac ggcgtggcgt cgatcgtctc
     3181 gtagaggagc gccgccttgg cgcgattgac cttctcgatc tccgccaggc cgccttgctc
     3241 cttcagccag cgaaagacca ggcccgacat gtagatcgca aacgtcggcg gcgtgttgat
     3301 catcgagttg ttggcggcct gcacggtgta gtcgaaggcc tgcggcgtcg tcttcgccgc
     3361 gccgcccagc aagtcgtcgc ggacgatcac gaaggtgagg ccggccgggc cgacgttctt
     3421 ct
//
LOCUS       DASRWS010000328         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4165750, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000328 DASRWS010000000
VERSION     DASRWS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165750"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFF72_06185"
     CDS             <1..404
                     /locus_tag="VFF72_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92787.1"
                     /translation="CAALADEAEGCEQLAIGMRARVIGLHHQTRAGTPGKIDIDALAA
                     LLRERQPADTYLAEAWWIVAQAFDALDRPQDAEGALRHAFEWVIGRALPNVPPAFRDS
                     FLHRNAANLSLLAGAAKRLGLRPPAAAITPG"
     gene            480..896
                     /locus_tag="VFF72_06190"
     CDS             480..896
                     /locus_tag="VFF72_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92788.1"
                     /translation="MTTMTIAALQWSRLRDLGDVAPLEEADLVCMAEIRAVLTRHHRL
                     GRFALHLVHKHFDVADDEVLVEYSDATMREQHLRVERRSDVRDAIPTTWTLESEQPAV
                     ACVCAWRLELGHLGRHAARRLQRADARPETRSGRRR"
     gene            complement(912..1598)
                     /locus_tag="VFF72_06195"
     CDS             complement(912..1598)
                     /locus_tag="VFF72_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZV92789.1"
                     /translation="MIKLYYHPSPNPAKVALFLEEAGLPYELVPIDTRKGEQHSPSFL
                     AINPNGKTPALTDGDVKVFDSNAILLYLAEKTGLFLPAATPENRAGMYSWLMFVATGI
                     GPYTGQNVHFRHYAPEPKAYAVNRYDFEAWRHWNLVDAQLAKGRYMLGDTYTLVDMAV
                     WGWARAVPFALGADAWDKLPHVKRLLDEINARPAAQRTEALKTRHAFKTDMDDDARKS
                     LFPQNARLAA"
     gene            complement(1595..2629)
                     /locus_tag="VFF72_06200"
     CDS             complement(1595..2629)
                     /locus_tag="VFF72_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZV92790.1"
                     /translation="MQSPPRRVVVLGTGGTIAGWAATPTETLAYRSAQLGVAELVAAA
                     PSTADTVVESEQVAQLDSKDMDFATWRRLALRVAHHLARPEVAGVVVTHGTDTLEETA
                     WFLARVVDATKPVVLTAAMRPASALDADGPGNLADAISLAGRAGHPGVVVTLAGAVHG
                     ARDVRKAHPQRPDAFTSGDAGPLARHDGTQWQWLRDAAAGPPSTGVDLLPEDESAWPW
                     VEVVTSDAGADGRVVDLLAGAGVDGIVVAATGNGSTHRLIAAALARAQASGVVVRRSS
                     RCVEGAIVETGNDGLPTAGGLSPVKARIELMLHLLCAGRPARSAERQQESGRGPRIAA
                     FPPNDQGPAS"
BASE COUNT          430 a          944 c          958 g          416 t
ORIGIN      
        1 cctgcgcggc gctcgccgac gaagccgagg gatgcgagca gctcgccatc ggcatgcgcg
       61 cacgcgtcat cggcctgcac caccagacac gggcaggcac gcccgggaaa atcgacatcg
      121 acgcgctggc cgcgctcctg cgcgagcgcc agccggccga cacctacctg gcggaagcgt
      181 ggtggatcgt cgcccaggcc ttcgatgcgc tcgatcgccc gcaagacgcc gaaggcgcgc
      241 ttcgccacgc cttcgaatgg gtgatcggcc gcgccctgcc gaacgtgccg cccgcatttc
      301 gcgacagctt ccttcaccgc aatgccgcca acctgtcgct gctcgccggc gcggcaaagc
      361 gtctcggcct gcgaccgccg gcggcggcga taacgcccgg gtaacgagcc gccgccaagc
      421 ttgcggtgtc ggggcgcgcc ccaggcgcaa ttcctcgagc gacccaggga gaaggatcga
      481 tgacgaccat gacgatcgcc gcgctgcagt ggtcacggct gcgggacctg ggcgacgtgg
      541 cgccgctgga agaagccgat ctcgtctgca tggccgagat tcgcgccgtg ctgacccggc
      601 atcaccgcct cggccggttc gcccttcacc tcgtgcacaa gcacttcgat gtggccgacg
      661 atgaagtgct ggtcgaatac agcgatgcga cgatgcgcga gcagcacctt cgcgtcgagc
      721 ggcgcagcga cgtgcgcgac gccatcccga cgacctggac gctcgaatcg gagcagcccg
      781 cggtcgcctg cgtctgcgcc tggcgcctcg agctcggcca tctcggcagg cacgcggccc
      841 gccggctgca gcgtgccgac gcccggccgg aaacgagatc gggacgacga cggtgatcga
      901 gaccggcggc ctcaggccgc caggcgtgcg ttctgcggga agagcgactt cctcgcgtcg
      961 tcgtccatgt ccgtcttgaa tgcgtgccgt gtcttcagcg cctcggtccg ctgcgccgcc
     1021 ggccgggcgt tgatctcgtc gagcagtcgc ttgacgtggg gcagcttgtc ccaggcgtcg
     1081 gcaccgagcg cgaacggcac ggcacgggcc cagccccaca cggccatgtc gacgagcgta
     1141 taggtgtcgc cgagcatgta gcggcccttc gccagctgcg cgtcgacgag gttccagtga
     1201 cgccaggcct cgaagtcgta gcggttcacg gcataggcct tgggctccgg cgcgtagtgc
     1261 ctgaagtgga cgttttgccc cgtgtacggg ccgatgccgg tggccacgaa catcagccac
     1321 gaatacatcc cggcgcgatt ctccggcgtc gccgcgggaa ggaagagacc ggtcttctcg
     1381 gcgaggtaga gaaggatcgc gttgctgtcg aagaccttga cgtcgccgtc ggtcagcgcc
     1441 ggcgtcttgc cgttcgggtt gatggcgagg aacgacgggc tgtgctgctc tccctttcgc
     1501 gtgtcgatcg gcacgagctc gtatggcaag ccggcctcct cgagaaagag cgccaccttg
     1561 gcgggattgg gcgaaggatg gtagtagagc ttgatcacga ggcgggtcct tggtcgttcg
     1621 gcgggaaagc cgcgattctc ggaccgcgac cggattcttg ctgccgctct gcagatcgag
     1681 ccgggcgccc ggcacaaagc aggtgaagca tcagctcgat gcgagccttc accgggctga
     1741 ggccgcccgc cgtcggcaag ccatcgttgc cggtctcgac aatcgcgcct tcgacgcaac
     1801 gcgagcttcg ccggacgaca acgccggatg cctgcgcgcg tgcgagtgcc gcggcgatga
     1861 gccgatgcgt gctcccgttg ccggtcgcgg cgacgacgat gccatcgacg ccggcgccgg
     1921 ccaggaggtc gacgacgcgg ccgtcggcgc cggcgtcgct cgtcacgacc tcgacccagg
     1981 gccatgcgct ttcgtcttcg ggaagaagat cgactccagt cgacggtggg cccgccgcgg
     2041 cgtcgcgaag ccattgccac tgcgtcccgt cgtggcgcgc cagcggcccg gcatcgcccg
     2101 aggtgaatgc gtcgggccgc tgcggatgcg ccttgcggac gtcgcgggcg ccatgcaccg
     2161 cgccggcgag cgtgacgacg actcccgggt gtccggcccg gccggccagc gaaatcgcat
     2221 cggcgagatt gccgggcccg tcggcgtcga gggcagaggc aggacgcatc gccgcggtca
     2281 ggacgacggg ctttgtcgcg tcgacgaccc gggccaggaa ccacgccgtt tcctcgagcg
     2341 tgtcggtgcc gtgcgtgacg acgacgcccg cgacctccgg tcgcgccagg tgatgagcga
     2401 cgcgaagcgc gagccgacgc caagtggcga agtccatgtc cttgctgtcg agctgcgcga
     2461 cctgctcgct ttcgacgacg gtatccgcgg tggacggcgc cgcggcgacg agctcggcca
     2521 cgccgagctg tgccgagcga taggccagcg tctcggtcgg cgtcgccgcc catccggcga
     2581 tcgtgccgcc agtgccgagg acaacgacgc gacgcggcgg gctttgcata tttttcgaag
     2641 ctggatgaaa atacaggact ggataaatat tcagcgacgg cttttcgctg ccgccgcaac
     2701 cgcaggagtg gccatgcacg agacgcccaa gctcaccgat cgccagca
//
LOCUS       DASRWS010000329         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_136164, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000329 DASRWS010000000
VERSION     DASRWS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136164"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFF72_06205"
     CDS             complement(<1..259)
                     /locus_tag="VFF72_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92791.1"
                     /translation="MMSSAEPNSMSAAMKLAKAERDLSAAGAAGRSIHACRMLRAGVA
                     ESTRTLSRRLRLRGQISPQPLESTACVRHPVNPALERVVHAE"
     gene            231..1376
                     /locus_tag="VFF72_06210"
     CDS             231..1376
                     /locus_tag="VFF72_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV92792.1"
                     /translation="MLFGSAEDIIVVAVGWLVFSRTRSTFDLGMIGLAGFLPLILLSL
                     LTGLVTDRFDRRAVLAICGAGLCAGAVMLCVASAGDAIWPIYVIVVLIGSAKAFLGPV
                     SKALVPNLVSAGDLTRGFAMAHSFGGTARLLAPALGGLLYAFGPQVPFAAASVLAALG
                     VASCIGIGARPPENVTSGVSWSTLIAGFVFIWSAPVVMAAMGLDLVAVLLGGATALMP
                     YYAQEIFHVGPWALGLMRTAPAVGGLLMSGMLAWWPLQRHAGPTLLAVVVIYGLGTIG
                     FGLSTTLPIALACLLILGAADAVSMVVRAGLVQTQTPDAMRGRVSAVHSLFTGASGEL
                     GEVESGLLSSAIGVVPCVVLGGVAAIVAAAAWAALVPALRRADRFEA"
     gene            complement(1392..>2371)
                     /locus_tag="VFF72_06215"
     CDS             complement(1392..>2371)
                     /locus_tag="VFF72_06215"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZV92793.1"
                     /translation="ALTSSFPPKRINADFTVSSENSFNSRLNSIAADTETLLDRLMEE
                     APLDGERSRPKRLLDSIRYSSLGAGKRFRPFLVVESAALFGVPHAQALMVGAALECVH
                     CYSLVHDDMPAMDNDDLRRGRPTTHKAFDDATAILAGDSLLTYAFDIVARPETHPSAD
                     VRVKLVMALARASGLGGMVGGQILDLAAEGRFADVPRPQTLADVETLQAMKTGALIRF
                     GCNAGAILGQTDDARYRALDTYGAAVGQAFQIADDLLDIEGDTATVGKQTGKDAAAGK
                     ATIVGVLGIAKSKERLKSLVDEANSAVSVFGASAEVLKAAARFVAERQT"
BASE COUNT          295 a          797 c          848 g          431 t
ORIGIN      
        1 actcggcgtg tacgacacgt tcgagcgcgg ggtttaccgg atggcggacg caagccgttg
       61 actccaacgg ttgcgggctg atttgcccac gcaggcgcag gcggcgtgat agcgtccgcg
      121 tcgattcggc aacaccggcc ctcaacatcc gacacgcgtg aatggatcgt cctgcagcac
      181 cggccgcgga aaggtcgcgc tcggccttcg ccagtttcat cgctgccgac atgctgttcg
      241 gctcggccga ggacatcatc gtggtcgcgg tcggctggct ggtgttcagc cggacccgca
      301 gcacgttcga tctcggcatg atcggcctcg ccggcttcct gccgttgatc ctgctgtcgc
      361 tcctgaccgg gctcgtgaca gatcgcttcg accggcgagc ggtgctcgcg atatgcggcg
      421 ccgggctgtg cgccggcgcg gtcatgcttt gtgtcgcgtc agccggtgac gcgatctggc
      481 cgatctacgt catcgtggtg ctcatcgggt ccgccaaggc gttcctcgga ccggtcagca
      541 aggcgctggt gcccaatctg gtttcggccg gcgacctcac ccgcgggttc gcgatggcgc
      601 actcgttcgg cgggacggcg cgactgctgg cgccggcgct gggcggtcta ctctacgcct
      661 tcggcccgca ggtgccgttt gccgccgcga gcgtgctcgc tgcgctcggc gtcgcaagct
      721 gcatcggcat cggggcacga ccgcccgaga acgttacgtc cggcgtcagt tggtcgacgc
      781 tgatcgccgg gttcgtgttc atctggtcgg cgccggtcgt catggcagcg atggggctcg
      841 acctcgtcgc ggtgctgctc ggcggcgcga ccgcgctgat gccgtactac gcccaggaga
      901 tctttcatgt cggcccgtgg gcgctcggcc tgatgcggac ggcaccggcc gtcggcgggc
      961 ttctcatgtc gggcatgctg gcctggtggc cgctgcagcg gcacgccgga ccgacgctgc
     1021 tcgccgtcgt cgtcatctac ggtctcggca ccatcggctt cggcctgtcg accacgctgc
     1081 cgatcgcgct ggcgtgcctt ctgatcctcg gcgccgccga tgcggtcagc atggtcgtgc
     1141 gcgcggggtt ggtgcagacg cagaccccgg acgcgatgcg cggccgcgtc agtgcggtcc
     1201 actccctgtt caccggcgcg tcgggcgagc tcggtgaagt cgagtccgga ctgctctcgt
     1261 cggcaatcgg cgtggtgccg tgcgtcgtgc tcggcggcgt cgccgcgatc gtggcggctg
     1321 ccgcctgggc cgctttggtc ccggcgttac gccgggccga tcgcttcgag gcttagtctg
     1381 gtctaacgcc gtcaggtctg ccgctcggcc acgaaccgcg ccgcggcctt gagcacctcg
     1441 gcgctcgcgc cgaagaccga caccgctgag tttgcctcat cgactaggct cttgagccgc
     1501 tccttcgact tggcgatgcc gagcaccccg acgatggtcg ccttgccggc agcggcatcc
     1561 ttgccggtct gcttgccgac cgtcgcggtg tcgccctcga tatcgaggag atcatcggcg
     1621 atctggaacg cttggccgac cgcggcgccg taggtgtcga gcgcgcggta gcgcgcgtcg
     1681 tccgtctgcc cgagaatcgc gcccgcgttg cagccgaagc ggatgagcgc gccggtcttc
     1741 atcgcctgca gcgtctcgac gtcggcgagg gtctgcggtc gcggcacgtc ggcaaagcgg
     1801 ccctcggccg cgaggtcgag gatctggccg ccgaccatgc cgccgaggcc ggacgcgcgc
     1861 gcgagcgcca tcacgagctt gacgcgcacg tcggcgctcg gatgcgtttc gggccgcgcc
     1921 acgatgtcga acgcgtaggt gagcaggctg tcgccggcga ggatcgcggt ggcgtcgtcg
     1981 aacgccttgt gcgtcgtcgg gcggccgcgg cgcaggtcgt cgttgtccat cgccggcatg
     2041 tcgtcgtgca ccagcgaata gcagtgcacg cattcgagcg cggcgccgac catcagggcc
     2101 tgcgcgtgcg gcacgccgaa cagggcggcc gactcgacca ccaggaaggg gcggaagcgc
     2161 ttgccggcgc cgaggctcga atagcggatc gagtcgagca ggcgtttcgg ccggctgcgc
     2221 tcgccgtcga gcggcgcttc ctccatcagc cggtcgagca aggtctcggt gtcggccgcg
     2281 atcgagttga gacgggaatt gaagctgttc tcgctgctca cggtgaaatc cgcattgatc
     2341 cgcttgggtg gaaaggacga ggtcagggct t
//
LOCUS       DASRWS010000330         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2713703, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000330 DASRWS010000000
VERSION     DASRWS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713703"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFF72_06220"
     CDS             complement(<1..694)
                     /locus_tag="VFF72_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZV92794.1"
                     /translation="MHRSSVAAALVAIAAVCGPPAEAQSLLYGLIDAAGSRSRPPGGD
                     YRYQLDNGDLSRSFIGFRGSEDLGGGLRAVYKLESYLQINNGDVGRYGGDTFFGRDSN
                     VGLSGAFGTTVLGRSATPFYQATVNFNPFGESYVFSPSTRQYYAGALVSDRSWNNSLS
                     YSNNASDAPLRINFAVNTSNEIDTRRNVGGSIAYITGPFAATLALEQVRNTPLPVPAA
                     FKREFAVQAGATY"
     gene            917..1354
                     /locus_tag="VFF72_06225"
     CDS             917..1354
                     /locus_tag="VFF72_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZV92795.1"
                     /translation="MPLLWALRDALDLTGTKFGCGVAACGACTVHVDGRAVRSCVLPL
                     EAVRGKQVTTIEGLGSADAPHPLQAAWVAHQVPQCGYCQSGMLMAAAALLASNRNPSD
                     ADIDAAITNICRCGTYPRVRAAIHTAAGQLRRNAAAAMPVRLA"
     gene            1351..>2726
                     /locus_tag="VFF72_06230"
     CDS             1351..>2726
                     /locus_tag="VFF72_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZV92796.1"
                     /translation="MRRRALLVGGLGAAGALVVGWLALPPRDRLGGVDSLPVHDGEVG
                     LNGWIKIAGDGGVVLAMNRSEMGQGTHTALPMLVAEELDVPLARVRLVTAPNDRLYGN
                     VSAMVASVPWFHPSDIEPGHETLEARGGRWVVAKVARELGINLTGGSSSVVDAWDVLR
                     LAAATARAQLVGAASLTWKQPASELTVADGVVRHASGLSAHYGQLARVAASTPSGEVR
                     LKLPSQWKLIGTPAPRVDVVAKANGSAVFGIDARPAGLVHAAIRHCPMIGGGIGAVDV
                     APALALPGVERLVRLGPYGGSTDALAVVARTGWHARQGAAAIGVDWQQRPSGGLDSKE
                     IVETLVRTADAAARRGGGFVFHSRGDIDAAEARAASRVEQSYSAPYLAHAAMEPINCT
                     ARVADGRVEVWVSTQVPGLARAIAAEVAGVEPDAVTVHVPYLGGAFGRRLEVDVVGQA
                     VRIALECGG"
BASE COUNT          377 a          939 c         1015 g          395 t
ORIGIN      
        1 cgtaggtggc gccggcctgg accgcgaact cccgcttgaa ggcggccggc accggcagcg
       61 gcgtgttgcg gacctgctcg agggccagcg tcgcggcgaa cggccccgtg atgtaggcga
      121 tgctgccacc gacgttgcgc cgggtgtcga tctcgttcga ggtattgacg gcgaagttga
      181 ttcgcagcgg cgcatcgctc gcgttgttcg agtaggacag cgaattgttc cagctccggt
      241 cgctgaccag cgcgcccgcg tagtactggc gcgtgctggg ggagaacacg taggactcgc
      301 cgaacggatt gaaattgacc gtcgcctggt agaacggcgt cgcgctgcgc cccagcaccg
      361 tggtgccgaa cgcacccgaa aggcccacgt tcgaatcgcg gccgaagaac gtgtcgccgc
      421 cgtagcgacc gacatcgccg ttgttgatct gcaggtacga ctcgagcttg tagacggcac
      481 gcaggccgcc gccgaggtcc tcgctgccgc gaaagccgat gaagctgcgc gacaggtcgc
      541 cgttgtcgag ctggtagcga tagtcgccgc cgggcggcct gctgcggctg cctgcggcgt
      601 cgatcaggcc gtaaagaagg ctttgcgcct cggccggcgg gccgcagacg gcggcgatgg
      661 cgaccagcgc ggccgcgact gacgagcgat gcattcgtga tacctcggcg cgaaccggaa
      721 gaaggaaacg aagcgcggga ccgatggcgt gcacccggtc gcgcgatgcg caaggcgcga
      781 tgccggttgc ccggtcggcc gtattctggc gcaccggcac aatccggcgg cccgccctga
      841 gggcaaaagg agacatgtgg agcttgtcgt caacggcgaa tcgcatcgcc tggccgagga
      901 cctggcgccg ccgcgcatgc cgctgctgtg ggcgcttcgc gacgcgctcg atctgaccgg
      961 cacgaaattc ggctgcggcg tcgcggcctg cggcgcgtgt accgtgcacg tcgatggccg
     1021 cgcggtccgc tcctgcgtgt tgccgctcga ggcggtccgc ggcaagcagg tgacgacgat
     1081 cgaaggcctc ggcagcgccg acgcgccgca cccgctgcag gcggcgtggg tggcgcacca
     1141 ggtgccgcaa tgcggctact gccagagcgg catgctgatg gcggccgcgg cactgctggc
     1201 gtcgaaccgc aacccgagcg atgccgacat cgacgcggcg atcaccaaca tctgtcgctg
     1261 cggcacctat ccgcgcgtac gcgccgcgat ccacaccgcg gccgggcagt tgcgtcgcaa
     1321 cgccgccgcc gcaatgccgg tccgcctggc atgagacgcc gcgcgctgct cgtcggcggg
     1381 ctcggcgccg ccggcgccct cgtcgtcggc tggctcgcgt tgccgccgcg cgatcggctg
     1441 ggcggtgtcg acagcctgcc cgtgcacgac ggcgaagtcg ggctcaacgg ctggatcaag
     1501 atcgccggcg acggcggcgt cgtgctggcg atgaatcgca gcgaaatggg ccagggaacg
     1561 cacaccgcgc tgccgatgct cgtcgccgag gagctcgacg tgccgctggc gcgggtgcgt
     1621 ctcgtcacgg cgccgaacga ccgcctctac ggcaatgtct cggcgatggt cgcgagcgtg
     1681 ccgtggtttc atccgagcga catcgagccg ggtcacgaga cgctggaggc gcgcggcggc
     1741 cgctgggtgg tcgccaaggt ggcgcgcgag ctgggcatca atctcaccgg cggctcgtcg
     1801 agcgtcgtcg atgcatggga cgtgctgcgc ctggcggcgg cgacggcgcg tgcccagctc
     1861 gtcggcgcgg cgtcgttgac ctggaagcag cccgccagcg agctgaccgt ggccgacgga
     1921 gtcgttcgcc acgcctccgg cctgtcggcg cactacggcc agctggcccg cgtcgccgca
     1981 tcgacgccga gcggcgaggt ccgcctcaag ttgccttcgc aatggaagct catcggcacg
     2041 ccggcgccac gggtcgacgt cgtcgccaag gcgaacggct cggcggtctt cggcatcgat
     2101 gcccggccag ccgggctggt ccacgccgcg atccgccatt gccccatgat cggcggcggc
     2161 atcggcgccg tcgatgtcgc accggcactc gctctgcccg gtgtcgagcg gctcgtccgg
     2221 ctcgggccgt acggcggctc gaccgacgcg ctggccgtcg tcgcgcgcac cggctggcat
     2281 gcgcgccagg gggcggcggc gatcggcgtc gactggcagc agcgtccgtc cggcggcctc
     2341 gacagcaagg agatcgtcga gacgctggtt cgcaccgccg acgcggcggc ccggcgaggc
     2401 ggcggcttcg tctttcattc gcgcggcgac atcgacgcgg ccgaggcgcg cgcggcgtcg
     2461 cgggtcgagc agtcgtacag cgcgccctat ctcgcgcacg cggcaatgga gccgatcaac
     2521 tgcaccgccc gcgtcgccga cggccgggtc gaggtctggg tgtcgacgca ggtgccgggg
     2581 ctggcgcgtg ccattgccgc cgaggtcgcc ggcgtcgaac ccgacgccgt gacggtgcac
     2641 gtcccgtatc ttggcggcgc cttcggtcgc cggctcgagg tcgacgtcgt cggccaggcg
     2701 gtgcgcatcg cgctggaatg cggcgg
//
LOCUS       DASRWS010000331         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2423600, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000331 DASRWS010000000
VERSION     DASRWS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423600"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFF72_06235"
     CDS             complement(<1..167)
                     /locus_tag="VFF72_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZV92797.1"
                     /translation="MNHVFESLARRFGRILLAGVAAALVAGCGYNDFQRLDEQVKASW
                     SEVLNQYQRRA"
     gene            485..1495
                     /gene="phnD"
                     /locus_tag="VFF72_06240"
     CDS             485..1495
                     /gene="phnD"
                     /locus_tag="VFF72_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV92798.1"
                     /translation="MGQALWAAMAALFFAFASPAHAADCPHGELDSAYCVDGDKMVAK
                     PPADPKDWKDPSTIVFAYTPVEDPAVYANAFKPFVNYLSSCTGKKVVYYPVLSNSAEV
                     EAMRSGRLHVAGFSTGPTAFAVNLAGAIPFAIKGDPKAYESYRMIMLVRADSKFHKLT
                     DLKGARVAHTAASSNSGNLAPRALFPELGLVPDKDYKVIYSGGHDRTVLGIKSGDYDA
                     GTVASDVFERMAARGDIKESDFRIIYSSDPFPTSGFAYAHDLKPELAQKIVKCFLDFR
                     FPPEMQKEFAGGVDRFWPATYLKDWAVIRKVADAAHAPYDRAAFDAEAKKEAAKLEKK
                     KN"
     gene            1514..2290
                     /gene="phnC"
                     /locus_tag="VFF72_06245"
     CDS             1514..2290
                     /gene="phnC"
                     /locus_tag="VFF72_06245"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HZV92799.1"
                     /translation="MNDVVAGSGSALIVEGLRKEYVAGRPVLRGIDLAIAQHGLTAII
                     GPSGTGKSTLIRCINRLVEPSAGRLLFHGVDLASLDRRALRRARRRIGMVFQEYNLVE
                     RLTVMENVLSGRLGYVSPWKAWLRRYPPDDVELAFDLLDRVGLAAQYQQRADALSGGQ
                     RQRVGIARALMQRPELLLADEPTSSLDPRTAVEIMELIAGLGRELAIPVIVNIHNVEL
                     ARRYADRVIGMKNGEIVFDGAPEALEEAQLKDIYGGEDWL"
BASE COUNT          419 a          769 c          719 g          389 t
ORIGIN      
        1 tcggcgcggc gctggtactg gttcagcacc tccgaccacg acgccttcac ctgttcgtcg
       61 agacgctgga agtcgttgta gccgcagccg gcgaccaggg ccgcagcgac accggcgagc
      121 aggatccggc cgaagcgtcg ggccagcgac tcgaagacat gattcatcgc gggatccttt
      181 tctggaggga aggcgggcga tcgtataccg acgctcgtgc tcccccggga gtgatggaca
      241 tgacggagtc caccggcacc acgattgtca ggcgcgtatg cacaagaagc ctcacacgac
      301 tatcgcattc gagctcgcga ccgtccacgc attggggccg gcgagacgcc ggtcacggtg
      361 accgtcattg gccgaatggt cgttcctctc gattgacacc gacatcgggt cccgccagca
      421 ttgttcgagg tatttgcgta cctcatttct cagaacagca cttattacga gggagaaaga
      481 cacgatgggc caggcactct gggcggcgat ggccgccctg ttttttgcat tcgcttcgcc
      541 ggcgcacgcc gccgactgcc cgcacggcga gctcgactct gcctactgtg tcgatggcga
      601 caagatggtg gccaagcctc cggccgatcc caaggactgg aaggatccgt cgacgatcgt
      661 cttcgcctac acccccgtcg aagacccggc cgtctacgcc aacgcattca agccgttcgt
      721 caactacctc tcgagctgca ccggcaagaa ggtcgtctat tacccggtcc tttcgaattc
      781 ggccgaggtc gaggcgatgc gctcgggccg cctccacgtc gcgggcttct cgaccggccc
      841 gacagcgttt gccgtcaacc tcgccggcgc cattccgttc gcgatcaagg gcgatccgaa
      901 ggcgtacgag agctaccgga tgatcatgct ggtgcgcgcg gattccaagt tccacaagct
      961 caccgacctc aagggagccc gggtcgcgca caccgcggca tcgtcaaact ccggcaacct
     1021 cgcgccacgt gcgctcttcc cggagctcgg cctcgtcccc gacaaggact acaaggtgat
     1081 ctactcgggc ggacacgacc ggacggtcct cggcatcaag tcgggtgact acgacgccgg
     1141 caccgtcgcg tccgacgtgt tcgaaagaat ggcagcgcgc ggcgacatca aggaatccga
     1201 cttcaggatc atctacagca gcgatccgtt cccgacctcc gggttcgcct acgcgcacga
     1261 cctcaaaccc gagctcgcgc agaagatcgt caagtgcttc ctcgatttcc gcttcccgcc
     1321 ggagatgcag aaggagttcg ccggcggggt cgaccgcttc tggccggcga cctacctgaa
     1381 agattgggca gtgatccgca aggtcgccga tgcggcccac gcaccctacg accgcgcggc
     1441 gttcgatgcc gaagcaaaga aggaagcggc caagctcgag aagaagaaga actgacgttc
     1501 cggatgtcga acgatgaatg atgtcgttgc cggcagcggc agcgcgctga tcgtcgaggg
     1561 tcttcgcaag gagtacgtgg ccggaaggcc ggtgctgcgc ggaatcgacc tcgcgatcgc
     1621 gcaacacggc ttgaccgcca tcatcggccc gtcgggcacc ggcaagagca cgctgatccg
     1681 ctgcatcaac cggctcgtcg agccgagcgc aggacgcttg ctgttccacg gtgtcgacct
     1741 ggccagcctc gatcggcgtg ccctgcgccg tgcgcgccgg cgcatcggca tggtgttcca
     1801 ggaatacaac ctcgtcgagc ggctgaccgt gatggagaac gtgctctccg gtcgcctcgg
     1861 ctacgtctcg ccctggaagg cctggctgcg gcgctatcca cccgacgatg tcgagctcgc
     1921 gttcgacctg ctcgaccgcg tcggcctggc cgcgcagtac cagcagcgcg cagacgccct
     1981 gtccggtggc cagcgccagc gcgtcggcat cgcccgggcg ctgatgcaac gccctgagct
     2041 gctgctcgcg gacgagccga cgtcgtcgct cgaccctcgc accgcagtcg agatcatgga
     2101 gctgattgcc gggctcggtc gcgagctcgc gatcccggtg atcgtcaaca tccacaacgt
     2161 cgagctcgct cgccgctatg ccgatcgcgt catcggcatg aagaacggcg agatcgtctt
     2221 cgacggtgcg ccggaggcgc tcgaggaagc gcagctcaag gacatctacg gcggcgagga
     2281 ctggttgtga tcgacg
//
LOCUS       DASRWS010000332         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_138530, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000332 DASRWS010000000
VERSION     DASRWS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138530"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFF72_06250"
     CDS             <1..252
                     /locus_tag="VFF72_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HZV92800.1"
                     /translation="NQVSAAGEGRTIRNQLQLVVDRFVVPQLKAAGETTPIVLELLGE
                     IGSDPAVREAVQKRSLLLAMQPGSAPAQAVNAIALRLAA"
     gene            complement(221..925)
                     /locus_tag="VFF72_06255"
     CDS             complement(221..925)
                     /locus_tag="VFF72_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZV92801.1"
                     /translation="MKGTPKSAAWSELATLRARLTEHRRHEEALAAQAAAAAVEQGAG
                     QSLFAESVGAVVPLNKGAAASPPRPRPPPRARQRERDEASALAESIADVFDAETLLET
                     DDALSFRRRGIGPDVVRKLRRGVWALQDDLDLHGLRREEAQDRLAVFLREATRAGYRC
                     VRVVHGKGHGSPGREPVLKDKVRRWLAHNAAVLAFTYARAADGGHGALIVLLRSAGEA
                     AASAAPVRRRDAARSR"
     gene            complement(922..1596)
                     /gene="radC"
                     /locus_tag="VFF72_06260"
     CDS             complement(922..1596)
                     /gene="radC"
                     /locus_tag="VFF72_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZV92802.1"
                     /translation="MFKDIPVSARPRERLLALGPAALADAELIALLLRTGLPGIGVLQ
                     LAQQLLDAFGGLAGLLRAGPDELGRVKGLGGTAKRAEMAAVLELARRSVAGELAARPV
                     FDAPGKVKDYLALQLAGRRHEVFAVLFLDAQSRLVALEEMFRGTLTQTSVYPREVVKR
                     ALDLHAAAVILAHNHPSGAAEPSRADEYLTQTLKSALALVDVRVLDHLVVGHGTVVSF
                     AERGLL"
     gene            1729..2178
                     /locus_tag="VFF72_06265"
     CDS             1729..2178
                     /locus_tag="VFF72_06265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZV92803.1"
                     /translation="MKRIEPGSFLTLHYRVTGPEGAAVIDTFGVQPATLSLGTGELAP
                     AMEACLIGLEEGSRASFDLPPGKAFGERSQALVRRLSKAALAGIGDADADYAVGDVVR
                     FPTPDGQASFAGVVRVVEDDGCIFDFNHPLAGRAVTFEVEVVGVLAG"
     gene            2201..3190
                     /gene="ispH"
                     /locus_tag="VFF72_06270"
                     /gene_synonym="lytB"
     CDS             2201..3190
                     /gene="ispH"
                     /locus_tag="VFF72_06270"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZV92804.1"
                     /translation="MNDPLPTPGSTAVEEVVLAEPRGFCAGVDRAIEIVERALLKFGP
                     PIYVRHEIVHNTHVVDDLKRKGAVFIEDLAEVPPGATLIFSAHGVSQAVRAEAAARGF
                     SVFDATCPLVSKVHIEVAKLAREGYEFIMIGHKGHPEVEGTMGQLDHGIHLVEDVADV
                     AGVDIAPGRKLAVVTQTTLSVDDAAEILAAVKRRFPDVREPKQQDICYATQNRQDAVK
                     VLAPAVDVVIVVGSPSSSNSNRLRELAERLGADAYMVDAAVDLRAEWLEGKRRVGVTA
                     GASAPEVLVQEVIERLRGLGAVSVRSLPGATETVHFPLPMGLGDKSMGALGRG"
     gene            complement(3247..3516)
                     /locus_tag="VFF72_06275"
     CDS             complement(3247..3516)
                     /locus_tag="VFF72_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92805.1"
                     /translation="MQFLIVALVVAGCFGYAAWTLMPSPARRWMARHMATWPLPARVL
                     AKLRRQAESSGGCSCDGCDKSAHAKTKTAARPPVAPVTFHPRRRP"
     gene            complement(3521..>3973)
                     /locus_tag="VFF72_06280"
     CDS             complement(3521..>3973)
                     /locus_tag="VFF72_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZV92806.1"
                     /translation="LLRLCGVDAHAVAGAAGPAIQYSFAGMLGQLLQHVFAPIGFNWQ
                     ISIALVPGLAAREVAVGALGTVYAMSAAGDSVADALSPVIAHGWSMATAFSLLAWYVF
                     APQCISTLAVVKRETNSWRYPIIMAAYLFALAYIAAFATYRITLWLGG"
BASE COUNT          614 a         1381 c         1428 g          550 t
ORIGIN      
        1 aaccaggtca gcgccgcggg cgaaggccgg acgatccgca accagctgca gctcgtcgtc
       61 gatcgcttcg tcgtcccgca actgaaggcc gcgggcgaga cgacgcccat cgtgctcgag
      121 ctgctcggcg agatcggcag cgaccccgcg gtgcgcgaag cggtgcagaa gcgctcgctc
      181 ctgctcgcga tgcagccggg cagcgcgccg gcgcaggccg tcaacgcgat cgcgctgcgt
      241 ctcgccgcct gacgggcgcg gccgacgcgg ccgcttcgcc agccgagcgc aacagtacga
      301 tcagcgcgcc gtgaccgccg tcggcggcgc gtgcataggt gaaggcgagc acggccgcgt
      361 tgtgggccag ccagcgccgc accttgtctt tcagcaccgg ctcgcggccg ggcgagccgt
      421 gccccttgcc gtggacgacg cggacgcagc ggtagccggc gcgcgtcgcc tcgcgcagga
      481 agacggcgag ccggtcttgc gcctcctcgc gacgcaggcc gtgcaggtcg aggtcgtcct
      541 gcagcgccca gacgccgcgg cgaagcttgc gcacgacgtc ggggccgatg ccgcggcggc
      601 gaaaggaaag cgcatcgtcg gtctcgagca gcgtctcggc gtcgaagacg tcggcgatcg
      661 actcggcgag tgccgatgcc tcgtcacgct cgcgctggcg cgcccgtggc ggcgggcgtg
      721 gtcgcggtgg cgaggccgcg gcccccttgt tcagcggtac gacggcgccg acgctttcgg
      781 cgaacagcga ctggccggca ccttgctcga cagcggcggc cgcagcctgc gcggcgaggg
      841 cttcttcgtg ccggcgatgc tcggtgagcc gcgcgcgcag cgtcgcgagc tcgctccacg
      901 ccgccgactt cggcgtgccc ttcacaggag gccccgctcg gcgaacgaga ccaccgtgcc
      961 gtggccgacg accaggtgat cgaggacgcg cacgtcgacc agcgccagcg cgctcttcaa
     1021 ggtctgcgtc aggtattcgt cggcgcgcga gggctccgcc gcgcccgacg gatggttgtg
     1081 agcgaggatc accgcggcgg cgtgcaggtc gagcgcgcgc ttgaccactt cgcgcgggta
     1141 gacgctcgtc tgcgtcagcg tgccgcgaaa catctcctcg agcgcgacga gacgactttg
     1201 ggcgtcgagg aagagaacgg cgaagacttc gtgtcgacgg cccgcgagtt gcaaggcgag
     1261 gtagtccttg accttgccgg gcgcatcgaa caccggccgt gccgccagct cgcccgccac
     1321 cgatcggcgg gccagctcga gcaccgcggc catctctgcg cgcttggcgg tgccgcccag
     1381 gcccttgacg cgcccgagct cgtcgggccc ggcgcgcagc agcccggcaa ggccgccgaa
     1441 cgcatcgagg agctgctgcg cgagctgaag cacgccgatg ccgggcagcc cggtgcgcag
     1501 aagaagcgcg atcagctcgg catcggccag cgcggccggc cccagcgcca gcagtctttc
     1561 gcgcggtctc gccgagacgg gaatgtcctt gaacatgtgg gggcggccgc gcacggacgc
     1621 ggtcgcgagg ctctctagaa tcgacgccag tctatgccgc ctcgacgaca gcaccgcccc
     1681 ccttcggcgc tcgtccttgc gcaccccctt caagagcgaa atccatccgt gaaacgaatc
     1741 gagcccggct cgttcctcac cttgcactac cgcgtgacgg ggcccgaagg cgctgccgtc
     1801 atcgacacct tcggcgtcca gccggcgacg ctgtcgctcg gtaccggaga actggcgccc
     1861 gccatggagg cgtgcctgat cggcctcgag gaaggcagcc gtgcgtcgtt cgacctgccg
     1921 ccgggcaagg ccttcggcga gcgaagccag gcgctggtgc ggcgcctcag caaggcggcg
     1981 ctcgccggca tcggcgatgc cgatgccgac tatgccgtgg gcgacgtggt gcgcttcccg
     2041 acgcccgacg gccaggcgag cttcgccggg gtggtgcgcg tcgtcgagga cgacggctgc
     2101 atcttcgact tcaaccatcc gctcgccggc cgcgcggtga ccttcgaggt cgaggtcgtg
     2161 ggcgtgctgg ccggatgagc gcgagaggcc tggacgcgac atgaacgatc cgctgccgac
     2221 acccggaagc accgcggtcg aggaagtcgt cctcgccgag ccgcgcggct tttgcgccgg
     2281 cgtcgaccgg gcgatcgaga tcgtcgagcg cgcgctgctc aagttcgggc cgccgatcta
     2341 cgtgcgccac gagatcgtcc acaacaccca cgtcgtcgac gacctcaagc gcaagggcgc
     2401 cgtcttcatc gaggacctcg ccgaggtgcc gcctggcgcg acgctcatct tcagcgccca
     2461 cggcgtctcg caggcggtgc gcgccgaggc ggcggcgcgc ggcttcagcg tcttcgatgc
     2521 gacctgtccg cttgtcagca aggttcacat cgaggtggcc aagctggctc gcgagggcta
     2581 tgaattcatc atgatcgggc acaagggcca tcctgaagtc gaagggacga tggggcagct
     2641 cgaccatggg atccatctcg tcgaggatgt cgccgacgtt gccggtgtcg acatcgcgcc
     2701 gggccgcaag ctcgcggtcg tgacgcagac gacgctctcg gtcgacgacg cggccgagat
     2761 cctggccgcc gtgaagcggc gttttcccga cgtgcgcgag cccaagcagc aggacatctg
     2821 ctacgcgacc cagaaccgcc aggacgcggt gaaggtgctg gcccctgcag tcgatgtcgt
     2881 gatcgtcgtc ggcagcccga gcagctcgaa cagcaaccgg ctgcgcgagc ttgccgagcg
     2941 gctgggcgcc gatgcctaca tggtcgatgc cgcggtcgac ctgcgcgccg agtggctcga
     3001 gggcaagcgc cgggtcggcg tcaccgcagg cgcgtcggcg cccgaagtgc tcgtgcagga
     3061 ggtcatcgag cggctgcgtg gcctcggggc cgtttcggtt cgcagcctgc cgggagccac
     3121 ggagacagtg cacttcccgt tgccgatggg gcttggcgac aagtcgatgg gggcgctcgg
     3181 acgcggctga cccggcgacc ggcgttccgc tcctttccgt ggacgacagg cgtccgccgg
     3241 aaacgctcag ggccggcgtc gtggatggaa cgtgaccggc gcgaccggag gacgagcggc
     3301 tgtcttcgtc tttgcgtgag ccgacttgtc gcagccgtcg caactgcagc cgcccgacga
     3361 ttccgcctgg cggcgcagtt tggcgaggac cctggccggc aatggccagg tcgccatgtg
     3421 ccgggccatc cagcgccgcg ccggcgacgg catgagcgtc cacgccgcat agccgaagca
     3481 gccggcgacg acgagggcga cgatcaggaa ctgcatcact tcatcctccg agccacagcg
     3541 tgatccggta ggtcgcgaac gcggcgatat acgccagcgc gaagagatac gcggccatga
     3601 tgatcggata gcgccaggaa ttcgtttcgc gcttgacgac ggcgagcgtc gagatgcact
     3661 gcggcgcgaa cacgtaccag gcgagcaggg aaaacgccgt cgccatcgac cagccgtggg
     3721 cgatcaccgg cgagagcgca tcggccaccg aatcgccggc cgccgacatc gcgtagacgg
     3781 tgccgagcgc gccgaccgcg acctcgcggg ccgccaggcc gggcaccagg gcgatcgaga
     3841 tctgccagtt gaagccgatc ggcgcgaaca cgtgctgcag gagctggccg agcatgccgg
     3901 cgaagctgta ctggatcgcc gggccggccg cgccggcgac ggcatgagcg tccacgccgc
     3961 atagccgaag cag
//
LOCUS       DASRWS010000333         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1002979, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000333 DASRWS010000000
VERSION     DASRWS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002979"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFF72_06285"
     CDS             complement(<1..563)
                     /locus_tag="VFF72_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HZV92807.1"
                     /translation="MLRPFGPDLYVAEGPTVSFFGFPYPTRMAVARLSSGQAWVWSPV
                     ALTAELARSVAAIGPVGCIVSPNKLHHLFLPEWKDRWPDARLFAPPGLARKKKGVRFD
                     ATLADQADESWAHDIDQVVFRGSLAMDEVVFFHRASRTAIFGDLIQRFSEDASRGWKG
                     LLLRLDGVAGPHGSTPREWRLSFVSRKV"
     gene            736..1527
                     /locus_tag="VFF72_06290"
     CDS             736..1527
                     /locus_tag="VFF72_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZV92808.1"
                     /translation="MNSPLRIERTGPVARLWLDRPEIRNALNAQVIRELAAAFAAFSA
                     DDSGVRAIVLGGSGKAFCAGADLGFMREMGGYTWEQNRADAEVLAEMLWTMYRCPVPV
                     VARIHGDCYAGGMGLAAVCDIRVVAEPATFCLSEARLGLLPATISPYVIRAMGEAQAS
                     RWFVTAERFGAAEAKAMGFAHEVCALEAIDAKVDALVATLVANGPMAARACKRLVRDV
                     AGREITPELRAETARRIADIRASAEGKEGVQSFLEKRKPGWLLPS"
     gene            1524..2132
                     /locus_tag="VFF72_06295"
     CDS             1524..2132
                     /locus_tag="VFF72_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZV92809.1"
                     /translation="MNGDLTQLVAVAAALGWASGLRLYAVVFLTGLAGAVGWVDLPGG
                     LRVLQSPWLLGASGAMLAMEFLADKIPGLDSVWDAVHTLVRIPAGAALAAAVFGGDDA
                     SWRVAAGLLGGTLAATSHVAKATTRAAVNTSPEPFSNIALSLLGDGFVPFLLWLSWVH
                     PLAALALIALVVVVSVLVGIALARFLSALWRRWRRPSLATGT"
     gene            2226..>2558
                     /locus_tag="VFF72_06300"
     CDS             2226..>2558
                     /locus_tag="VFF72_06300"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559822.1"
                     /note="catalyzes the formation of acetoacetate and
                     acetyl-CoA from 3-hydroxy-3-methylglutaryl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZV92810.1"
                     /translation="MLPSRVTLVDVGPRDGLQNEKQTVDAAVKIELVHRLQAAGLKEI
                     EVTSFVSPNWVPQMSDNAAVLAGIERQPGVRYSVLVPNMKGLDAALSAPRNQWPDEIV
                     VFAAATEAF"
BASE COUNT          354 a          915 c          905 g          384 t
ORIGIN      
        1 accttgcgcg acacgaagct gaggcgccat tcgcgcggcg tgctcccgtg cggcccggcc
       61 acgccgtcga gacgcaacag cagccctttc cagccccggc tcgcgtcctc ggaaaatcgc
      121 tggatcaggt cgccgaagat cgccgtgcgc gacgcgcggt ggaagaacac cacttcgtcc
      181 atcgccagcg atccgcggaa caccacctga tcgatgtcgt gggcccacga ctcgtccgcc
      241 tggtcggcaa gcgtcgcatc gaagcggacg cccttcttct ttcgcgccag gcccgggggt
      301 gcgaagagcc gcgcgtcggg ccagcgatcc ttccactcgg gcaggaagag gtggtggagc
      361 ttgttcggcg agacgatgca gccgaccggt ccgatcgccg ccacgctcct ggccagctcg
      421 gccgtcaagg ccaccggcga ccacacccag gcctggccgc tcgagagccg cgcgacggcc
      481 atgcgcgtcg ggtacgggaa gccgaagaac gacaccgtcg gaccctcggc gacgtacagg
      541 tcgggtccga agggacgaag catcgatcca gcatagcgtc ttcgcgccga tcgccttcgg
      601 cgatccgcga accccatgtc gagagcagct cgggtcgaga agggccctcc gcccggtcga
      661 cgcctgccct ctcgaggtgg ggaggcgcac gcgtccgatt cacatcgcac cccggccgca
      721 cgcagaatga cggtcatgaa ctcaccgctt cgcatcgaac gcaccggccc ggtcgcccgg
      781 ctctggctcg accggcccga gatccgcaac gccctgaacg cccaggtgat ccgcgagctc
      841 gccgccgcgt tcgccgcgtt ctcggccgac gacagcggcg tgcgggcgat cgtcctgggc
      901 ggcagcggca aggcgttttg cgccggcgcc gatctcggct tcatgcgcga gatgggcggc
      961 tacacgtggg agcagaaccg cgccgatgcc gaggtgctgg ccgagatgct gtggacgatg
     1021 taccgctgcc cggtgccggt ggtggcgcgc atccacggcg actgctatgc cggcggcatg
     1081 ggcttggcgg cggtgtgcga catccgcgtc gtcgccgagc ccgccacctt ctgcctcagc
     1141 gaggcgcggc tggggctgct gccggcaacc atcagcccct acgtgatccg ggcgatgggc
     1201 gaggcgcagg cctcgcgctg gttcgtcacc gccgagcgct tcggcgcggc cgaggcgaag
     1261 gcgatgggct tcgcccacga ggtgtgcgcg ctcgaggcga tcgatgcgaa ggtcgacgcg
     1321 ctggtggcca cgctcgtcgc caacggaccg atggcggcgc gcgcctgcaa gcggctggtc
     1381 cgcgacgtgg ccggccgcga gatcacgccc gagctgcgcg ccgaaacggc gcggcgaatc
     1441 gccgacatcc gcgccagcgc cgagggcaag gaaggcgtgc agagcttcct cgagaagcgc
     1501 aagcccggct ggctgctgcc gtcgtgaacg gcgatctcac ccagctcgtg gccgtcgccg
     1561 ccgccctcgg ctgggcgagc gggctgcgcc tgtacgcggt cgtctttctc accggcctgg
     1621 ccggtgcggt cggctgggtc gatctgccgg gcggcctgcg cgtgctgcaa agcccgtggc
     1681 tgctcggcgc gagcggcgcg atgctggcca tggagttcct cgccgacaag attcccggcc
     1741 tcgacagcgt ctgggacgcc gtccacacgc tggtgcggat ccccgccggt gcggccctgg
     1801 cggcggcggt gttcggcggc gacgacgcga gctggcgcgt cgctgccggc ctcctcggcg
     1861 gcacgctggc cgccaccagc cacgtcgcca aggcgaccac ccgggcggcc gtgaacacat
     1921 cgcccgagcc tttttccaac atcgcgcttt cgcttctcgg cgacggcttc gtgccgttcc
     1981 ttctctggct gtcgtgggtc catccgctgg cggcgctggc gctgatcgcg ctggtggtcg
     2041 tcgtctcggt gttggtcggc atcgccctgg cgcgctttct ttcggcgctc tggcggcgct
     2101 ggcgccgccc ttcgctggcg acggggacat gacgccggca tcgcctcgct gcgcaggcgg
     2161 cccgtcgatc cgggccggcg cgggctggtt accatgacgc ggcgcctttc gaaggagatc
     2221 cccacatgct gccctcccgc gtgactctcg tcgacgtcgg tccgcgcgac ggcctgcaga
     2281 acgagaagca gaccgtcgac gcggcggtga agatcgagct cgtgcatcgc ctgcaggccg
     2341 ccggcctgaa agagatcgag gtgaccagct tcgtgagccc gaactgggtg ccgcagatga
     2401 gcgacaacgc ggccgtgctc gccggcatcg aacgccagcc cggcgttcgc tattcggtgc
     2461 tggtgccgaa catgaagggg ctcgacgccg cgctgtcggc gccgaggaac cagtggcccg
     2521 acgagatcgt cgtcttcgcc gccgccaccg aggcgttc
//
LOCUS       DASRWS010000334         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4167849, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000334 DASRWS010000000
VERSION     DASRWS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4167849"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFF72_06305"
     CDS             complement(<1..575)
                     /locus_tag="VFF72_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV92811.1"
                     /translation="MTSLTRLLALALAAALALALGACSATGTLNALARTDTYRLTADI
                     AYGALPRQRLDVYTPVSASPGAGWPVVVFFYGGNWTSGQRGEYKFMGEALASRGILTI
                     VADYRLYPEVTYPGFLEDCAKAMAWGFEHAKSLGGDPKRIFVMGHSAGAYNAAMLALD
                     PRWLGDARKAVRGFIGLAGPYDFLPFDQQATR"
     gene            complement(656..1048)
                     /locus_tag="VFF72_06310"
     CDS             complement(656..1048)
                     /locus_tag="VFF72_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92812.1"
                     /translation="MQRRQLIISLGAAAAAATTLTARGQGSTAGKRKPDLADVAEGRY
                     AGDVISDSKGSSHEGVTLIVRRIGINRVRIESDYPRLPIVEVTLTRALDKIVQARGNT
                     AFVYDWAKRPVRLDVSFDNEVSWSGSKE"
     gene            complement(1125..2522)
                     /gene="argH"
                     /locus_tag="VFF72_06315"
     CDS             complement(1125..2522)
                     /gene="argH"
                     /locus_tag="VFF72_06315"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZV92813.1"
                     /translation="MNNQFDRKSEAWSALFSEPTSELVKRYTASVSFDRRLWRADIAG
                     SRAHARMLAMQGVIGAADLAAIETGLTTVAGEIESGRFEWRADLEDVHMNIEARLIEL
                     AGDAGKRLHTGRSRNDQVATDVRLWLREQIDVVRGLLGDLQLALVTLAEQQVDTILPG
                     FTHLQVAQPVSFGHHLLAYVEMFSRDAERMAEVRARTNRLPLGAAALAGTSYPLDREA
                     VARELGMEGLCRNSIDAVSDRDFAIEFTAAAALSMVHVSRFSEELVLWMSQSFGFITL
                     ADRFTTGSSIMPQKKNPDVPELARGKSGRVIGHLMALLVLMKGQPLAYNKDNQEDKEP
                     LFDTVDTLVDTLRVFIEMVPGIRVSAEAMESAAARGFATATDLADYLVKKGVPFRDAH
                     EAVARTVKHAIGQGTALGDLSLATLQGFHPAIASDVHGVLTLRGSIASRSVAGGTAPA
                     QVKAEIVRHRARLGR"
     gene            2744..>3680
                     /locus_tag="VFF72_06320"
     CDS             2744..>3680
                     /locus_tag="VFF72_06320"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZV92814.1"
                     /translation="MNAPPRIEPSSTGFGATIFEVAAIAEPTRGGADSAFDVCHVGVV
                     LRALIFVHVAMAIGMVFSGLSLTVWFALVAAGSSVALPAVLAWLLVLCAMKRPLAATA
                     VWLQWTWAIGLGALAAWGSARLVVWVLPDATAVGLGALVGVGPPLAGAAMAAAIFVWL
                     RLRAQATLPAETTARLAELQSRIRPHFLFNTLNTALTLVRLDPARAEGVLEDLAELFR
                     VAIGDTAESVSLTEEVELAQRYLDIEQARFGSRLHVSWELDPEAGSARVPPLILQPLV
                     ENAVRHGVESRPEGATIEIEGRSVGSDVRLCIRDDG"
BASE COUNT          501 a         1276 c         1320 g          583 t
ORIGIN      
        1 cgggtggcct gttgatcgaa gggcaggaaa tcataggggc cggccaagcc tatgaagccg
       61 cgcaccgctt tcctggcgtc tcccagccag cgcggatcga gcgccagcat cgccgcgttg
      121 taggcaccgg cgctgtggcc catcacgaag atccgcttcg ggtcgccgcc gagcgacttc
      181 gcgtgctcga agccccacgc catcgccttg gcgcagtctt cgaggaagcc ggggtaggtg
      241 acctccgggt agaggcggta atcggcgacg atcgtcagga tgccgcgcga ggcgagcgcc
      301 tcgcccatga acttgtattc gccgcgctgg ccgctggtcc agttgccgcc gtagaagaag
      361 acgacgacgg gccagcccgc gcccggcgac gcggacacgg gcgtgtagac atcgaggcgc
      421 tggcgcggca gcgccccgta ggcgatgtcg gccgtgaggc gataggtgtc ggtgcgggcc
      481 agggcgttga gcgtccccgt ggcggagcag gcgccgagcg ccagggcgag cgccgcggcg
      541 agcgcgaggg cgaggagtcg ggtcagcgag gtcatcgcca tcgatacgag gcaatcggcg
      601 cgccggatgc gccgctcggg cggcggccga ttgcggtcgc tgccgcggcc gtgcgtcact
      661 ccttcgagcc gctccagctc acctcgttgt cgaagctcac gtcgagacgc acgggccgct
      721 tcgcccagtc gtagacgaac gcggtgttgc cgcgcgcctg gacgatcttg tcgagggctc
      781 gcgtcagcgt cacctcgacg atgggcaggc gcggatagtc ggactcgatc cgcacgcggt
      841 tgatgccgat tcgcctgacg atcagggtca cgccttcatg cgacgatcct ttcgaatcgg
      901 agatcacgtc gccggcatag cggccttcgg cgacgtcggc gaggtcgggt ttgcgcttgc
      961 cggccgtgct gccctggccc cgcgccgtca gcgtggtcgc cgccgccgcg gcggcaccca
     1021 gggaaatgat cagctggcgt cgctgcatgg gctcctcctg aggaatgtcg gtgtcgcgat
     1081 gctatcgaga cgcggcaggc gcggccggac gctgcgcgtc cggttcagcg gccgaggcgc
     1141 gcccggtgcc ggacgatctc ggccttcacc tgcgccggcg cggtgccccc tgcgacgctg
     1201 cgcgacgcga tcgagccgcg cagcgtcagc acgccgtgca cgtcggaggc gatcgccgga
     1261 tggaagccct gcaatgtcgc caaggacagg tcgccgagcg ccgtgccctg gccgatggcg
     1321 tgcttgaccg tgcgtgcgac ggcctcgtgg gcgtcgcgaa acggcacgcc tttcttgacg
     1381 aggtagtcgg cgaggtcggt cgccgtcgcg aagccgcgcg ccgcggcgct ttccatcgcc
     1441 tcggccgaga cgcggatgcc cggcaccatc tcgatgaaca cgcgcagcgt gtcgacgagc
     1501 gtgtcgacgg tgtcgaagag cggctccttg tcttcctggt tgtccttgtt gtaggcgagc
     1561 ggctggccct tcatgaggac gagcagcgcc atcaggtggc cgatgacgcg tccgctcttg
     1621 ccgcgcgcca gctcgggcac gtcggggttc ttcttctgcg gcatgatcga cgaaccggtg
     1681 gtgaagcggt cggccagcgt gatgaagccg aagctctggc tcatccagag caccagctcc
     1741 tcgctgaagc gcgacacgtg caccatggac agcgccgccg cggcggtgaa ctcgatcgcg
     1801 aagtcgcggt cgctgacggc gtcgatgctg ttgcggcaga ggccctccat gccgagctcc
     1861 cgcgccaccg cctcgcggtc gagcgggtag ctggtgccgg cgagcgccgc cgcgccgagc
     1921 ggcagccggt tggtgcgcgc acgcacctcg gccatgcgct cggcatcgcg cgagaacatc
     1981 tcgacatagg ccagcaggtg atggccgaac gacaccggct gggccacctg cagatgcgtg
     2041 aagccgggca ggatggtgtc gacctgctgc tccgccaacg tcaccagggc gagctgcaga
     2101 tcgccgagaa gcccgcgcac gacgtcgatc tgctcgcgca gccagaggcg aacgtcggtc
     2161 gccacttggt cgttgcgcga gcggccggtg tgcagtcgct tgccggcgtc gccggcgagc
     2221 tcgatcagcc gcgcctcgat gttcatgtgc acgtcttcga gatcggcgcg ccactcgaag
     2281 cggccgctct cgatctcgcc ggccacggtc gtcagccccg tttcgatggc ggccagatcg
     2341 gccgcgccga tgactccctg catcgccagc atcctggcgt gggcgcggct gccggcgatg
     2401 tcggcgcgcc agaggcgccg atcgaacgac acgctggccg tgtagcgctt cacgagctcg
     2461 ctcgtcggct ccgagaacag cgccgaccaa gcctcggatt tcctgtcgaa ctggttgttc
     2521 atggcgctcg gaaggggtcg tcgaatcgtg cgcacgtcac agatcggtgc cgacagcgac
     2581 cccgcaacga cgcacggcga ggcggcggtt ggcgacaatc tcgccatgcg ccccgtcagg
     2641 attgccgcga ttctatcggc gagccccctc gccgcatgag cgccaccacg aatcgcgtcg
     2701 ctcgtctgcg cggtcggggg ccgtcgcgac atcgagcgca tcgatgaacg ccccgccgcg
     2761 catcgagccg agctcgaccg gcttcggcgc caccatcttc gaggtcgcgg cgatcgccga
     2821 gccgacccgc ggcggtgccg actcggcgtt cgacgtctgc cacgtcggcg tcgtcctgcg
     2881 tgcgctcatc ttcgtgcacg tcgcgatggc catcggcatg gtcttttcgg gcctgtcgct
     2941 caccgtgtgg ttcgcgctcg tcgccgccgg ctcgagcgtc gcgctgcccg ccgtgctggc
     3001 gtggctgctg gtgctgtgtg cgatgaagcg tccgctcgcg gcgacggccg tctggctgca
     3061 atggacctgg gccatcggcc tcggtgcact cgccgcgtgg ggatcggcgc gcctcgtcgt
     3121 ctgggtgctg cccgacgcga ccgcggtcgg actcggcgcc ctggtcggcg tggggccgcc
     3181 cctggccggc gccgccatgg ccgcggcgat cttcgtctgg cttcgcctgc gcgcgcaggc
     3241 gacgttgccg gccgagacga cggcccggct ggccgagttg cagtcgcgga tccgtccgca
     3301 cttcctcttc aacacgctca acacggcgct cacgctcgtg cgcctcgatc cggcgcgcgc
     3361 cgaaggcgtg ctcgaggacc tggcggagct gtttcgcgtc gccatcggcg acaccgccga
     3421 gtcggtctcg ctcaccgagg aggtcgagct cgcgcagcgc tatctcgaca tcgagcaggc
     3481 gcgcttcggc agccgcctgc acgtgagctg ggagctcgat ccggaagccg gcagcgcccg
     3541 cgtaccgccg ctgatcctgc agccgctggt cgagaacgcc gtccgccacg gcgtcgagtc
     3601 gcggcccgag ggcgcgacga tcgagatcga ggggcgcagc gtcggctccg acgtgcggct
     3661 gtgcatccgc gacgacgggg
//
LOCUS       DASRWS010000335         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_445001, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000335 DASRWS010000000
VERSION     DASRWS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445001"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFF72_06325"
     CDS             complement(<1..324)
                     /locus_tag="VFF72_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV92815.1"
                     /translation="MQALAEQLFFDGNRHLLAHEYGSAEACFREAVATWPALGAAHAN
                     LAWLLDRGGDAGEAEACHRRALALAPGDVQVHLNFGAFLAARNRHLEAEAIYVRAVGL
                     DPGSAP"
     gene            complement(447..692)
                     /locus_tag="VFF72_06330"
     CDS             complement(447..692)
                     /locus_tag="VFF72_06330"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HZV92816.1"
                     /translation="MSKLDAEHRNAIPAQKFAFAKERKEPLENAQHVRNAIARFNQVE
                     GVTDAERDTAWKHIQTAAKKFGVDVKEKSWREIGAKK"
     gene            861..2147
                     /locus_tag="VFF72_06335"
     CDS             861..2147
                     /locus_tag="VFF72_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZV92817.1"
                     /translation="MNPIPRRADDAHVVTLANAVRVVVMPLPHLETASVSVFVRTGSS
                     HESRRQNGISHVVEHMAFKGTRTRTCQQINLQAERLGADVNAHTDKDHTAYHMRGRAR
                     DAAAFVRMLGDIVQASTFPEAELDRERQVLLQEFIEDEEEPISTAYKLFDKLCFGSHP
                     LAQPVIGSRANIRRLDRDELLAYVQQQYSGANVVVGIAGHVDVDAVVRDAEATFRSMR
                     AGLPNLVDAPAQGGGIASRALTGSAQTHLVFGLPLPTLLDEAHHAGVVAAALFGEGMS
                     SPLLDEIRERRGLAYYLSCSADIGELAGQFVVEASTAPEHADEFLAELKRLLEAQAGH
                     VDPVGLERAKNQIAVRRLRAWERPARRLEDAALDLLALGRVRSRDEMAERIEAVDAAQ
                     VRDAFTQMLLRPEAIAIAGRIRKGSVERAREIFALR"
     gene            complement(2431..2883)
                     /gene="aroQ"
                     /locus_tag="VFF72_06340"
     CDS             complement(2431..2883)
                     /gene="aroQ"
                     /locus_tag="VFF72_06340"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZV92818.1"
                     /translation="MKILLLNGPNLNLLGTREPATYGTTTLADVEASFAKEAAALGLE
                     AAAFQSNHEGQLIDRIHAARGEGVRFIVINPGAFTHTSVALRDALAGVAIPFIEIHIS
                     NVHAREGFRHHSYLSDIAAGVVVGLGTLGYRLALQAAAEKLRQPTPAA"
     gene            complement(2880..>3429)
                     /locus_tag="VFF72_06345"
     CDS             complement(2880..>3429)
                     /locus_tag="VFF72_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV92819.1"
                     /translation="GLAQDQFDTWTLFARAVLGLERGESLDLADPYGLIRSSGFADPA
                     RTVRIVLNVSLSQRTRTARQVSATGRSGGGVQHIALSTSDIFATMRRLRDSGVRFVPI
                     SANYYDDLLARLDLAEKEVRRMQALDIVYDRVDGGEYFHAYAEPFADRFFFEIVQRGA
                     YDGYGAVNAPARMASQEQSANP"
BASE COUNT          492 a         1194 c         1222 g          521 t
ORIGIN      
        1 cggggccgag cccgggtcca ggccgacggc acgcacgtag atcgcttccg cctcgaggtg
       61 ccggttgcgc gccgccagga aggcaccgaa gttgaggtgg acctggacgt cgcccggcgc
      121 cagcgccagg gcccgccgat ggcaggcttc ggcctcgccg gcgtcgccgc cgcgatcgag
      181 cagccaggcg aggttggcgt gcgccgcgcc gagggcgggc caggtcgcca cggcctcgcg
      241 aaagcaggct tcggcgctgc cgtactcgtg cgccaggagg tggcggttgc cgtcgaagaa
      301 gagttgctcg gcgagggctt gcatgacccg ccattgcagc gcgtcggcgc cgcccgaggc
      361 cggtcgataa gaggccggaa accgcgccag tcgcggcgct gcttcacgta agttttcgtg
      421 gcggccggcg cgccgccagg gcagggctac ttcttcgcgc cgatctcgcg ccaggacttc
      481 tccttcacgt cgaccccgaa tttcttcgct gccgtctgga tgtgcttcca ggcggtgtcg
      541 cgctccgcgt cggtgacgcc ctcgacctgg ttgaagcggg cgatcgcgtt gcgcacgtgc
      601 tgcgcattct cgagcggctc cttgcgttcc ttggcgaagg cgaatttctg ggccgggatg
      661 gcgtttcggt gctcggcatc gagcttgctc atggcgggtc tccggtggct gggactcgtc
      721 gggtccggca atcgccgcgc ctgcggacga ccctcgcttc cgtctcccac gcgcttgcgc
      781 ccgcgagccc aagcgcatgg ccggagggat gccggcgcgg ccgaggacgc ccggatcgct
      841 ccgctggcga caatcggcgg atgaatccga ttccacgccg ggccgacgac gcccacgtcg
      901 tcaccctcgc caacgccgtt cgcgtcgtcg tcatgccgct gccgcatctc gagacggcca
      961 gcgtcagcgt cttcgtgcgc accggcagca gccacgagtc gcgccggcag aacggcatca
     1021 gccacgtcgt cgagcacatg gcattcaagg gcacgcgcac gcgcacctgc cagcagatca
     1081 acctgcaggc cgagcgcctc ggcgccgacg tcaacgcgca caccgacaag gaccacaccg
     1141 cgtatcacat gcgcggccgc gcccgcgacg cggcggcttt cgtgcgaatg ctcggcgaca
     1201 tcgtccaggc cagcaccttt cccgaggccg agctcgatcg cgaacgccag gtcctcctgc
     1261 aggagttcat cgaggacgag gaggagccga tctcgaccgc ctacaagctg ttcgacaagc
     1321 tgtgcttcgg cagccacccg ctcgcccagc ccgtgatcgg ctcgcgcgcc aacatccgcc
     1381 gcctcgaccg cgacgagctg ctcgcctacg tgcagcagca gtacagcggc gccaacgtcg
     1441 tcgtcggcat cgccggccac gtcgatgtcg acgccgtcgt ccgcgacgcc gaggcgacgt
     1501 tccggtcgat gcgcgccggc ctgccgaacc tcgtcgatgc cccggcgcaa ggcggcggaa
     1561 tcgcttcgcg ggcgctcacc ggcagcgccc agacgcacct cgtcttcggc ctgccgctgc
     1621 cgacgctgct cgacgaagcg catcacgccg gcgtcgtcgc cgcggcgctc ttcggcgaag
     1681 gcatgagctc gccgctgctc gacgagatcc gcgagcggcg cggcctggcg tactacctgt
     1741 cgtgctccgc cgacatcggc gagctggccg ggcaattcgt cgtcgaggcg tcgacggcgc
     1801 ccgagcacgc cgatgaattc ctcgccgagc tcaagcgcct gctcgaggcg caggccgggc
     1861 acgtcgaccc ggtcggcctc gagcgggcga agaaccagat cgccgtgcgc cggctgcgcg
     1921 cctgggagcg gccggcgcgt cgcctcgagg acgcggcgct cgatctgctg gcgctcggcc
     1981 gcgtccgttc gcgcgacgag atggccgagc gcatcgaggc ggtcgacgcg gcgcaggtgc
     2041 gcgacgcgtt cacgcagatg ttgctgcgac ccgaggcgat cgccatcgcc ggacggatcc
     2101 gcaagggatc ggtcgagcgg gcgcgggaga tattcgcctt gcgctgacgg gaggtgccgg
     2161 ctgcctcggt cggggccgtg ctcgggccgg cgatgccacc gaccgactcc gcgaggccac
     2221 gagcgccctg tcgttttcgg cgagcagcgt ccgtcagggc atgcagcaat tcctgcattc
     2281 gctccggcaa agcgccttgc agacactcga cgaggtctgc cgtcgagtgg ttccgaagcg
     2341 agccggtcag cacgaaagtc gtcctctgat gcgccgcttc acgaagcggg tcgaaacgca
     2401 gcgacgtcga ctcgggccgc gggtgtcgcc tcaagccgcc ggcgtcggct ggcgcagctt
     2461 ttccgccgcc gcctgcagcg ccaggcgata gccgagggtg cccaggccga cgacgacgcc
     2521 ggcggcgatg tccgacaggt acgagtggtg gcgaaagcct tcgcgtgcgt ggacgttgct
     2581 gatgtggatc tcgatgaacg ggatggcgac gccggcgagc gcgtcgcgca gcgccacgct
     2641 ggtgtgcgtg aacgcgccgg gattgatgac gatgaagcgc acgccctcgc cgcgcgccgc
     2701 gtggatgcgg tcgatcagct gtccctcgtg gttgctctgg aaggcggccg cctcgaggcc
     2761 gagcgctgcc gcttccttcg cgaacgacgc ctccacgtcg gcgagcgtcg tcgtgccgta
     2821 ggtcgccggc tcgcgcgtgc cgagcaggtt caggttgggt ccgttgagga ggaggatctt
     2881 catgggttcg cgctctgttc ctgcgacgcc atccgcgccg gcgcattgac ggcaccgtag
     2941 ccatcgtaag ccccgcgctg cacgatctcg aagaagaagc ggtcggcgaa gggctcggcg
     3001 taggcgtgga aatactcgcc gccgtcgacg cggtcgtaga cgatgtcgag cgcctgcatg
     3061 cgtcgcactt ctttctcggc gaggtcgagg cgggcgagca ggtcgtcgta gtagttggcc
     3121 gagatcggca cgaagcgaac gccgctgtcg cgcagccgac gcatcgtcgc gaagatgtcg
     3181 ctcgtcgaca gcgcgatgtg ctgcacgcct ccgccggagc ggccggtcgc actcacctgg
     3241 cgcgcggtcc gcgtccgctg gctgagcgag acgttgagga cgatgcgcac ggtgcgtgcc
     3301 ggatcggcga agccgctcga acggatcagg ccgtacgggt cggcgagatc gaggctctcg
     3361 ccgcgctcga ggccgagcac ggcgcgggcg aagagggtcc aggtgtcgaa ctggtcctgg
     3421 gccaagccg
//
LOCUS       DASRWS010000336         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_445488, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000336 DASRWS010000000
VERSION     DASRWS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445488"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2974
                     /locus_tag="VFF72_06350"
     CDS             <1..>2974
                     /locus_tag="VFF72_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZV92820.1"
                     /translation="HFYRQGLADLSRAGFSGAWLLAQLLRLAAISLDAAARALWRTYV
                     SRRRLLEWTTAAAAQAKATTAFLPLLRRHAGITALALLIWIGLAAADTPYPGWTAVLC
                     IAWALSPVWTWWVSRPRAVPRIDAPSAEDRALFSGAARDTWRLFERCVTPADNDLPPD
                     NLQTVPHDMIAHRTSPTNVGLYLLATACARSFGWIGRVEMVERIEATLRTLARLQRHR
                     GHFLNWYDTVGASPLLPLYVSTVDSGNLCIHLLTVAQACLELAASGDDGALRRGVEAS
                     KARCLVELASTALPSLDPALMGIVDAADPLAELRADPHGFGLRIEAAAAALDLLVEPG
                     EVGAPPAPRQLWIWAVHDHLSTLRSALRDIGAQDGAIAARLRDTAATCQRLACEPDFS
                     FLFHQKRRLFHIGFRVAEHQLDAGFYDLLASESRSTGLWAIAKGDVPAAHWAALGRPF
                     FAVGALAGLRSWSGSMFEYLMPTLVLDEPNGSVLDNAARAAVLEQIAYARVHHVPWGI
                     SESAYAGSDHTLAYQYAPQGVPKLALRRTPADELVIAPYATALAAQVAPHRAAANLHR
                     VEQTRSRMRYGFIESLDYTPARQSAGEAMTRVHTFMAHHQGMSIVAFANVLLDGTARR
                     WGMSDPRIEAVSSLLHERAPREVPVLREAPARPAPGSSPRRGPGILHEIVPGMVAIEP
                     THALSNGRYAVSLRANGAGSSRWNGLEISRARDDALRDACGMFFYLRWDRQPRPVSLT
                     QRPAPDRAAHYQATFQADRVMLSASWQEIQATTTVWVSPEDDIEFRRVELRNLGDRQL
                     DVELMSVFDVTLADARADESHPAFQNLFVGAEWQPAHQALVFERTPRLASEKGALAAN
                     FIADAEPPPSGVRIQVDRRRWLGRNRSPSHPLASFDEAPDAGDGGEAATLDTGLDPVS
                     ALAVRLQIAPRAKARITFCVAAADNPTTLRAIIDKYRQPTHAERASMMSATLAGIRLR
                     EMGLSVENHVAIQTLTTKL"
BASE COUNT          403 a         1131 c          986 g          454 t
ORIGIN      
        1 tccatttcta tcgccagggc ctggccgacc tgtcgcgcgc cgggttcagc ggagcctggc
       61 tgctcgcgca gctgcttcgc ctggccgcga tctcgctcga cgctgccgcc cgcgccctgt
      121 ggcgcaccta tgtgagccgg cgccgcctgc tcgagtggac caccgccgcc gcggcacaag
      181 ccaaggcgac gacggcgttc ctgccgctgc tgcgccggca tgccggcatc acggctctgg
      241 cgctgctcat ctggatcggc ctcgccgcgg ccgacacgcc gtacccgggc tggacggccg
      301 tcctgtgcat cgcctgggcg ctgtcgccgg tgtggacgtg gtgggtgagc cggccgcggg
      361 ccgtgccgcg catcgatgcc ccctccgcgg aagaccgcgc cctgttcagc ggcgccgccc
      421 gcgatacctg gcggctcttc gagcgctgcg tcacgccggc cgacaacgac ctgccgcccg
      481 acaacctgca gacggtgccg cacgacatga tcgcgcatcg cacgtcgccc accaacgtcg
      541 gcctctatct gctcgcgacc gcgtgcgcac ggtcgttcgg ctggatcgga cgcgtcgaga
      601 tggtcgagcg gatcgaggcg acgctgcgca cgctggccag gctgcagcga caccgtggcc
      661 acttcctcaa ctggtacgac accgtcggtg cctcgccgct cctgccgctc tacgtctcga
      721 cggtcgacag cggcaacctc tgcatccacc tactgacggt ggcgcaggcc tgcctcgagc
      781 tggccgcgag cggcgacgac ggggcactgc gtcgcggcgt cgaggcgtcg aaggcacgct
      841 gtctcgtcga gctcgcgtct acggcactgc cgtcactcga cccggcgctt atgggcatcg
      901 tcgacgcggc cgacccgctg gccgagctgc gcgccgatcc gcacggtttc gggctgcgca
      961 tcgaggcggc cgcggccgct ctcgacctgc tcgtcgagcc cggcgaggtc ggcgcgccgc
     1021 cagcgcctcg tcagctctgg atctgggccg tgcacgatca tctttcgacg ctgcgttccg
     1081 cgctgcgcga catcggcgcg caagacggtg cgattgccgc ccgccttcgc gacaccgctg
     1141 cgacgtgcca gcggcttgcc tgcgagcccg acttcagctt cctgttccac cagaagcgtc
     1201 gtctcttcca catcggattt cgcgtcgccg agcatcagct cgatgccggc ttctacgacc
     1261 tcctcgcgtc cgagtcgcgc tccaccggcc tgtgggccat cgccaagggc gacgttccgg
     1321 cagcgcactg ggcggcgctc gggcggccgt tcttcgccgt cggtgcgctc gccgggctgc
     1381 gctcgtggtc aggatcgatg ttcgaatacc tgatgccgac gctcgtcctc gacgagccga
     1441 acggcagcgt cctcgacaac gcggccaggg ccgccgtgct cgagcagatc gcctatgcgc
     1501 gcgttcatca cgtgccctgg ggcatctccg agtcggctta cgccggcagc gaccacacgc
     1561 tggcctacca gtacgcgccg caaggcgtgc cgaagctggc gctgcgccgc acgccggccg
     1621 acgagctggt catcgctccc tacgccaccg ccctggccgc tcaggtggct ccgcaccggg
     1681 ctgctgccaa cctgcaccgt gtcgagcaga cgcgatcgcg gatgcgctac gggttcatcg
     1741 aatcgctcga ctacacgccg gcccgccagt cggccggcga ggcgatgacc cgcgtccata
     1801 ccttcatggc gcaccaccag gggatgagca tcgtcgcctt cgccaacgtc cttctcgacg
     1861 gcacggcgcg ccgctggggc atgagcgatc cgcgaatcga ggcggtgtcg tcgctgctgc
     1921 acgaacgcgc gccgcgcgag gtgccggtgc ttcgcgaggc gccggcacga ccggcgcccg
     1981 gctcgtcgcc gcgccgcggc cccggcatcc tccacgagat cgttcccggc atggtcgcga
     2041 tcgagccgac gcacgccttg tccaacggcc gctacgccgt ctcgttgcgc gccaacggtg
     2101 ccggcagcag tcgatggaac ggcctcgaga tctcccgcgc acgcgacgat gccttgcgcg
     2161 acgcctgcgg catgttcttc tacctgcgct gggaccgcca gcccaggccc gtctcgctga
     2221 cccagcggcc ggcgcccgat cgtgccgccc attaccaggc gaccttccag gcggaccggg
     2281 tcatgctctc cgcctcctgg caggagatcc aggcgacgac gacggtctgg gtcagccccg
     2341 aggacgacat cgagttccgg cgagtcgaat tgcgcaacct cggcgaccgc cagctcgacg
     2401 tcgagctgat gtcggtgttc gacgtcacac tggccgacgc gcgggccgac gaatcgcatc
     2461 ctgcgttcca gaacctcttc gtcggcgccg aatggcagcc ggcgcaccag gctctcgtct
     2521 tcgagcggac accccgcctc gccagcgaaa agggcgcgct cgccgcgaac ttcatcgccg
     2581 acgcggaacc gccgccgtcg ggcgtgcgga tccaggtcga ccggcgtcgc tggctcgggc
     2641 gcaatcgctc cccgagtcat ccgctggcgt cgttcgacga agcgccggac gcgggagacg
     2701 gcggcgaggc ggccacgctc gacacgggcc tcgacccggt gtcggcgctg gcggtgcgcc
     2761 tgcagatcgc gccgcgggcc aaggcgcgga tcacgttctg cgtcgccgcc gccgacaacc
     2821 cgacgacgct gcgcgccatc atcgacaagt atcgccagcc gacgcacgcc gagcgggcgt
     2881 cgatgatgtc ggcaacgctc gccggcatcc ggctgcgcga gatggggctg agcgtcgaga
     2941 accacgtcgc gatccagacc ctgaccacca agct
//
LOCUS       DASRWS010000337         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1589290, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000337 DASRWS010000000
VERSION     DASRWS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589290"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFF72_06355"
     CDS             complement(<1..241)
                     /locus_tag="VFF72_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZV92821.1"
                     /translation="MDDDDLLRYSRHLLLDEIGIEGQRRLLSSHALVIGAGGLGSPVA
                     LYLGTAGVGRITIVDGDRVDLTNLQRQIAHDLSRLG"
     gene            complement(307..1740)
                     /locus_tag="VFF72_06360"
     CDS             complement(307..1740)
                     /locus_tag="VFF72_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZV92822.1"
                     /translation="MGVKLKVAGWIAVGAVAGALTTLQFEASARSAVSQLPIEELQQL
                     AAVFGMIKTDYVEPVDEKKLINDAITGMVAGLDPHSQYFDKKSFKEFREATTGRFVGI
                     GIEMGMEDGLVKVVSPIEGSPAFRAGLKSGDLITKIDDTLVKGLTVDQAVKRMRGEPN
                     TKVVLTVFRKSEARTFPVSIVREEIRLQSVRVKMMDPGYAWVRVSQFQDRTVEDFATK
                     VGDLYKQNPDIKGIVLDLRNDPGGLLEGAVAISSAFLPADSVIVTTNGQIPDSKATFK
                     ASPEYYARRGGDPLKHLPEALKKVPLIVLVNEGSASASEIVAGALQDYHRGTVMGAQT
                     FGKGSVQTVRQLSPDTALKITTARYYTPSGASIQNKGIVPDIMLDETAEGNLFAALRT
                     READLQQHLSSGQGPEVKDPAREKAREEARKLFEADQAKAMMPKPPPEFGSADDFQLR
                     QALNQLEGKTVLVSKTAVARKAEIRAN"
     gene            2078..3532
                     /locus_tag="VFF72_06365"
     CDS             2078..3532
                     /locus_tag="VFF72_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZV92823.1"
                     /translation="MAGCAVGPDYVRPAVVVPAAYKESAGWKAAEPRPVDARHPWWTW
                     YGDATLDRLVADADAANQNIRQAEAQYRQAQAIADQARAGYFPGVGAGVSAGRGTSTT
                     TGRDVTSFGASLSASWVPDLWGAVRRAVESGEAGVAASADDLAAARLSIQAAVAVDYL
                     QLRYVDEQRELYANTIAAYEKALQLTRAQNAAGVALRSDVALAETQLKTAQAAGVDLD
                     AQRAQLEHAIAILTGRAPAAFGLPPVSPAAAFRARLPVIPASLPSELLEHRPDIAAAE
                     RRVALANANIGVAKAASYPALTLSASGGVAGATVATLIDTPSRIWSLGAALAQTVFDG
                     GLRRARTAQAEAAFDVASAQYKQTVLGGFQQVEDDLATLAVLDRETALQDQAVAAARL
                     AERLALAQYRGGTASYLSVVTAQTLALSNERTAVQLRSRQMATSVGLIVATGAGWSPG
                     NAAATAASSAASVAAAATASPTAAASTASISSGH"
     gene            3536..>4422
                     /locus_tag="VFF72_06370"
     CDS             3536..>4422
                     /locus_tag="VFF72_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV92824.1"
                     /translation="MQADPTSRRRPAALAAIGLGALALILLVWFWRSEAAQKRPAARA
                     APSVQATTVRVRKQAVPIFRTGVGTVVAQQSVTVKPRVDGELDRVFFREGQDVKAGQL
                     LAQIDPRTLHAQLAQVQAQKARDQAQLGNARMDLGRYSRLITQEAATQQQLDTQKALV
                     AQLEAAMKTDDAQINYAQVQLGFTRIVAPISGRVGARLVDAGNIVHAADPGGLVVINQ
                     IDPISVLFTLPEEAFQDINRALHASAEPLQVVVYPRDSNEPIGSGKLTLLNNQIDTST
                     GTVQMKATFANAGHALWPG"
BASE COUNT          649 a         1559 c         1524 g          690 t
ORIGIN      
        1 ggccgaggcg cgacaggtcg tgggcgatct ggcgctgcag gttggtgagg tcgacgcgat
       61 cgccgtcgac gatcgtgatg cggccgacgc cggcggtgcc gaggtagagg gcgaccggcg
      121 agcccaggcc gcccgcgccg atgacgaggg cgtgcgacga cagcagcctt cgctgcccct
      181 cgatgccgat ctcgtcgagg agcaggtggc gcgagtagcg gagcaggtcg tcgtcgtcca
      241 tcgtggtcgt cgccaatgaa aacgcccgtg cgggcacggg cgtttttcgg tcgggggcag
      301 gcagcgtcag ttggcccgga tctcggcctt gcgcgcgacc gccgtcttgc tgacgagcac
      361 ggtcttgcct tcgagctggt tgagcgcctg gcgcaactgg aagtcgtcgg cgctgccgaa
      421 ctccggcggc ggcttgggca tcatcgcctt ggcctggtcg gcttcgaaga gcttgcgcgc
      481 ttcctcgcgc gccttctcgc gcgccggatc cttgacctcg ggaccctggc cgctcgagag
      541 gtgctgctgg aggtcggcct cgcgggtgcg cagcgcggcg aacaggttcc cctcggcggt
      601 ctcgtcgagc atgatgtccg ggacgatccc cttgttctgg atcgacgcac cgctcggcgt
      661 gtagtagcgc gcggtcgtga tcttcagcgc cgtgtcggga gagagctggc gcacggtctg
      721 caccgaaccc ttgccgaagg tctgcgcgcc catcacggtg ccgcggtggt agtcctgcag
      781 cgcgccggcg acgatctcgc tggccgaggc cgaaccttcg ttgacgagga cgatcagcgg
      841 caccttcttc agcgcttcgg ggagatgctt gagcgggtcg ccgccgcgac gcgcgtagta
      901 ctcgggcgat gccttgaagg tggccttcga gtcggggatc tggccgttgg tggtgacgat
      961 caccgaatcg gcaggaagga acgccgacga gatcgccacg gcgccctcga gcaggccgcc
     1021 cgggtcgttg cgaaggtcga ggacgatgcc cttgatgtcg ggattctgct tgtacagatc
     1081 gccgaccttc gtcgcgaaat cctcgaccgt gcggtcctgg aactggctga cacgcaccca
     1141 cgcgtagccc gggtccatca tcttgacgcg cacgctctgc agccggatct cctcgcgcac
     1201 gatgctgacc gggaaggtgc gggcttcgct cttgcggaag accgtcagca cgaccttggt
     1261 attgggctcg ccgcgcatgc gcttgacggc ctggtcgacc gtcagtccct tgacgagggt
     1321 gtcgtcgatc ttcgtgatga ggtcgccgct cttcaggccg gcgcgaaacg ccggcgagcc
     1381 ttcgatgggg ctcacgacct tcaccaagcc gtcttccatg cccatctcga tgccgatgcc
     1441 gacgaagcgc cccgtcgtcg cctcgcggaa ttccttgaag ctcttcttgt cgaaatactg
     1501 cgaatgcgga tcgagcccgg ccaccatgcc ggtgatcgcg tcgttgatca gctttttctc
     1561 gtcgaccggc tcgacgtagt cggtcttgat catcccgaac acggcagcca gttgctgcag
     1621 ctcctcgatc ggcagctgcg agacggcgct gcgggcgctc gcctcgaact gcagcgtcgt
     1681 cagtgcgccg gcgaccgctc cgacggcgat ccagccagcg accttgagtt tgacccccat
     1741 gaacgacccc ttgagattcc ggccggcaac ggctgtgtca cgcgattggc gcaaagtgat
     1801 cctcagtata ttcggcgagc aaattgagag cgcgcgtccg gtcgcaaagt tgcatgtctt
     1861 tgtccacttt gcaagctgaa cggcgcaagc ggcggtcacg ccgaccgttc gtcggccgac
     1921 gctgacaaaa ctgtcatccg aaagacagcg gcatctccac ggtcgccgcc tagagtggcg
     1981 cctttcgccg gccttttccc gcctttttcc cgccatgtcc tcttcgcgcc ccctgcggcg
     2041 cccgcgtctg cgcttgtgcg ccgtctcctg cctgctcgtg gccggctgcg ccgtcggccc
     2101 cgactacgtg cgtccggcgg tggtcgtgcc ggcggcctac aaggaaagcg ccggctggaa
     2161 ggcggccgag ccgcgtcccg tcgacgcccg gcacccctgg tggacgtggt acggcgacgc
     2221 caccctcgac cgcctcgtcg ccgatgccga cgcggccaac cagaacatcc gccaggccga
     2281 ggcgcagtac cggcaggcgc aagcgatcgc cgaccaggcg cgggccggct actttccggg
     2341 cgtcggcgcc ggcgtttcag ccggacgcgg cacgtcgacg accaccggcc gcgatgtcac
     2401 gagcttcggg gccagcctga gcgcgagctg ggtgcccgat ctgtggggag cggtgcgccg
     2461 ggcggtggag tccggcgaag ccggggtcgc ggcgagcgcc gacgacctgg ccgcggcgcg
     2521 cctgagcatc caggccgcgg tggccgttga ctacctgcag cttcgctacg tcgacgagca
     2581 gcgcgagctc tacgccaaca cgatcgccgc ctacgaaaag gcgctgcagc tgacccgcgc
     2641 gcaaaacgcc gccggcgtcg ccttgcgctc cgatgtcgcc ctggccgaga cgcagctgaa
     2701 gacggcccag gcggcggggg tcgacctgga cgcccagcgg gcgcaactcg agcacgcgat
     2761 cgccatcctc accggccggg cgccggcggc cttcgggctg ccgccggtgt cgccggccgc
     2821 cgcgtttcgc gcccgcctgc cggtgatacc ggcgagcctg ccttccgagc tgctcgagca
     2881 tcggcccgac atcgccgccg ccgagcggcg ggtcgccctc gccaacgcca acatcggcgt
     2941 cgccaaggcg gcgtcgtatc cggcgctgac gctttccgcc tcgggcggcg tggccggggc
     3001 gacggtggcg acgctcatcg acacgccgag ccggatctgg tcgctcggcg ccgcgctggc
     3061 gcagacggtc ttcgacggcg gcctgaggcg ggcccgcacg gcccaggccg aagcggcttt
     3121 cgacgtcgcc tcggcacagt acaagcagac ggtgctgggc ggcttccagc aggtcgagga
     3181 cgatcttgcg accctcgccg tcctcgaccg cgagacggcg ctgcaggacc aggccgtggc
     3241 ggccgcccgg ctcgccgagc gcctggcgct ggcccagtac cggggcggca cggcgagcta
     3301 cctgagcgtc gtcacggcgc agacgctggc cttgtcgaac gagcgcacgg ccgtgcagct
     3361 gcgcagccgg cagatggcaa ccagcgtcgg gctcatcgtc gcgaccggcg ccggctggtc
     3421 gccgggcaac gccgccgcga cggccgcgtc gtcggcggca tccgtggcgg ctgcggcgac
     3481 ggcttcgccg acggcagccg cgtcgacggc ttcgatctct tcaggacact gatccatgca
     3541 ggccgatcca acctcacgtc gccgccccgc cgccctcgcc gccatcgggc tcggcgcgct
     3601 ggcgctgatc ctgctcgtct ggttctggcg cagcgaggcg gcgcagaaaa ggcctgcggc
     3661 gcgggcggca ccgagcgttc aggcgacgac ggtccgcgtc aggaagcagg cggtgccgat
     3721 ctttcgtacc ggcgtcggca ccgtggtcgc ccagcagagc gtgacggtca agccgcgtgt
     3781 cgacggcgag ctcgatcgcg tcttcttccg cgaagggcag gacgtcaagg ccggacagct
     3841 gctggcccag atcgacccgc gaaccctgca tgcgcagctg gcacaggtgc aggcgcagaa
     3901 ggcgcgcgac caggcccagc tcggcaacgc ccgcatggac ctcggccgct acagccgcct
     3961 catcacgcag gaagcggcga cgcagcaaca gctcgacacg caaaaggcgc tcgtcgccca
     4021 gctcgaggcg gccatgaaga ccgacgacgc gcagatcaac tacgcgcagg tgcagctcgg
     4081 cttcacgcgc atcgtcgcgc cgatcagcgg ccgcgtcggc gcccgcctgg tcgatgcggg
     4141 caacatcgtt cacgccgccg atccgggcgg cctcgtcgtc atcaaccaga tcgatccgat
     4201 cagcgtcctc ttcacgctgc cggaggaggc gttccaggac atcaatcgcg ccctgcacgc
     4261 cagtgccgag ccgctgcagg tcgtcgtcta tccgcgcgac agcaacgagc cgatcggcag
     4321 cggcaagctg accctgctca acaaccagat cgacacctcg accggcacgg tgcagatgaa
     4381 ggccacgttc gccaacgccg ggcacgcgct ctggcccggc ag
//
LOCUS       DASRWS010000338         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_716798, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000338 DASRWS010000000
VERSION     DASRWS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716798"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFF72_06375"
     CDS             <1..328
                     /locus_tag="VFF72_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HZV92825.1"
                     /translation="DPTMAMQPGMITSVEPGLYRPGRWGVRVENLVLAVPASAGVSSE
                     FGEFLEFETLTLCPIDVRCLDASLLRADEIAWLDAYHGMVRERLSPLVAGDALAWLLK
                     RTAPLA"
     gene            492..671
                     /locus_tag="VFF72_06380"
     CDS             492..671
                     /locus_tag="VFF72_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92826.1"
                     /translation="MISRLTASAAVFAILAAATLTFAADVQQQRHVAARAVDVTVSPD
                     FVQLPPVLVIGHRHH"
     gene            1012..>2878
                     /locus_tag="VFF72_06385"
     CDS             1012..>2878
                     /locus_tag="VFF72_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZV92827.1"
                     /translation="MPLLDSQRLLPALAGFVISAALLWAGAEMLGLARGHGARPGRLL
                     RAAGIALAGCGLWWPGHFLEPAEHAGGAFAGIAFVAGLLALALAALAGATAAAATRKT
                     LPAFIGLAMLTLVVAYGHSVFATPGLFRAAGAAGTWGPGIAVMVLATALVAVVLDFKQ
                     GRTARAARIGAAAVGAAVLTLAAASPAEPGAVLALQWTEALLVAALAAFGILFVETSG
                     QPPHRDPEPARRQTSAAAPSVDSLTQLPTRVYFEDRLAAAATKADAGATRLALLFIDL
                     DGFKPVNDTYGHSIGDLVLEQVGQRLKAMSRGKDVVARVGGDEFLLLLTNVQTTESVA
                     QVASRLIQGLSHPYAVDGREVMISCSVGIAMYPDGCSHAKLIARADAAMYASKRAGGS
                     NHCFYSPAMDADAEAQFTLLRDLRKAVAAKEFELFYQPKIDARSGKVTAVEALLRWKH
                     PTNGILLPNVFIPIAERFGLIGHIGNWVIEESCRQSRQWRDKGLRMRVAINLSAHQMR
                     QDDIVERITGALETHKIHPSLLTCEITESAAMEDTKTTQATFRRLGELGTHLSIDDFG
                     TGYSSLAYLRKLPAEELKIDRSFIMDLEHSADARAVVDAVIKLAHALGLKVVAEGV"
BASE COUNT          407 a         1065 c          959 g          447 t
ORIGIN      
        1 cgatccgacg atggcgatgc agccggggat gatcaccagc gtcgagcccg gcctctatcg
       61 gcccggccgc tggggcgtgc gggtcgaaaa cctggtgctg gcggtaccgg catcggccgg
      121 cgtgtcgagc gagttcggcg aattcctcga gttcgagacg ctgacgcttt gtcccatcga
      181 cgtgcgctgc ctcgacgcct cgctgctgcg tgccgatgag atcgcctggc tcgacgcgta
      241 ccacggcatg gtccgcgagc gactctcgcc gctcgtcgcc ggcgacgcgc tggcctggct
      301 tctgaagaga acggcgccgc tcgcctgatc cttttgccgc ccggcaacgg gcagcggcgc
      361 agcgcgttcg tcggcgcccg ccgcagttcg tcggcacgat cggccgattc gtcgcaagac
      421 gctttgcgcg tcgacacccg gctcctagag ttcgcttcat cgacaccgat gcagcccacc
      481 aggagcccgc catgatcagc cgcctcaccg cctccgccgc cgtcttcgcg atccttgccg
      541 cggccaccct caccttcgcc gccgacgtgc agcaacagcg ccacgtcgcc gcccgcgccg
      601 tcgacgtcac ggtgtcgccc gacttcgtgc agctgccgcc ggtgctggtc atcggccatc
      661 gccaccactg aacgacatcg atcggcgcag cccgggtcga accgccgggt cgcgtcagac
      721 tggaaccggc gccccgaggc gccgggcacg agggccgcgc gggcaactgc gcggcccttg
      781 tcgttcctgc ggggcgaaag cgtgcggtat tcaacgccgg cgagatcgcc gccgcgccgg
      841 agggacgtcg cggcggcaaa gcgtcgatag gctgcggcac ccgcgagcgt ttccgccgtg
      901 ggccgagccc tcggcgatgc cgctcaaggt atggccggca ataccgaaat ggagaggttc
      961 ggtggtctcg cgcgaactcc ttcatccgac gctgcccggc cctgcttttt catgcccctt
     1021 ctggatagcc aacgcctgct gcctgcgctc gccgggttcg tgatcagcgc tgcgctgctg
     1081 tgggcgggtg ccgagatgct cggcctcgcg cggggccatg gcgcgcgccc gggtcgactg
     1141 cttcgcgcgg ccggcatcgc gctggcaggc tgcggcctgt ggtggcccgg ccacttcctg
     1201 gagccggccg agcacgccgg cggcgcgttc gccggcatcg ccttcgtcgc cggtcttctc
     1261 gccctcgcgc ttgccgccct ggccggagcg accgcggcag cagcgacccg caagacgctg
     1321 cctgccttca tcggcctggc catgctgaca cttgtcgtcg cctacggcca ctccgtgttc
     1381 gcgacgcccg gcctctttcg cgccgcaggg gccgcgggta cctggggacc gggcatcgcc
     1441 gtcatggtcc tcgcgacggc actcgtcgcc gtcgtgctcg acttcaagca agggcgcacc
     1501 gcccgggctg cgcgcatcgg cgccgctgcc gtcggtgccg cggtgttgac gctggccgcg
     1561 gccagcccgg ccgagccggg ggccgtgctt gccttgcaat ggaccgaggc gctgctcgtc
     1621 gccgcgctgg cggccttcgg catcctcttc gtcgagacga gcggccagcc gccgcatcgc
     1681 gatccggagc cggcgcgccg ccagacgtcg gcggcggcgc cctcggtcga cagcctgacg
     1741 cagttgccga cacgggtcta cttcgaagac cgcctcgccg ctgccgcgac caaggccgac
     1801 gccggcgcga cccggctcgc cctgctcttc atcgacctcg atggcttcaa gccggtcaac
     1861 gacacctacg gccattcgat cggcgacctc gttctcgagc aggtcggcca gcgcctgaag
     1921 gcgatgtcgc gcggcaagga cgtcgtcgcc cgcgtcggcg gcgacgagtt cctgctgctg
     1981 ctgacgaacg tgcagacgac cgaatcggtc gcgcaggtgg cgagccgcct gatccagggc
     2041 ctgtcgcacc cctacgcggt cgacggccgt gaagtgatga tctcgtgctc ggtcggcatc
     2101 gccatgtatc ccgacggctg cagccacgcc aagctgatcg cccgcgccga cgccgcgatg
     2161 tacgcgagca agcgcgccgg cggctcgaac cactgcttct actcgccggc catggacgcc
     2221 gacgccgagg cgcagttcac gctcctgcgc gacctgcgca aggcggtcgc ggccaaggag
     2281 ttcgagctgt tctaccagcc caagatcgat gcgaggagcg gcaaggtcac cgcggtcgag
     2341 gcgctgcttc gctggaagca tccgaccaac ggcatcctgc tgcccaatgt cttcatcccg
     2401 atcgccgagc gcttcggcct gatcggccac atcggcaact gggtgatcga ggagtcgtgc
     2461 cggcaaagcc ggcaatggcg cgacaagggc ctgcgcatgc gcgtggccat caacctctcg
     2521 gcgcaccaga tgcgccagga cgacatcgtc gagcgcatca ccggcgcgct cgaaacgcac
     2581 aagatccacc cgtcgctgct cacctgcgag atcaccgagt cggcggcgat ggaggacacg
     2641 aagacgacgc aggcgacttt ccggcgcctc ggcgagctcg gcacgcacct gtcgatcgac
     2701 gacttcggta ccggctactc gagcctcgcc tacctgagaa agctgccggc cgaggagctc
     2761 aagatcgacc gcagcttcat catggatctc gagcacagcg ccgacgcgcg cgccgtcgtc
     2821 gacgccgtca tcaagctcgc ccacgcgctc ggcctgaagg tcgtcgccga aggcgtcg
//
LOCUS       DASRWS010000339         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_141079, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000339 DASRWS010000000
VERSION     DASRWS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141079"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..497)
                     /locus_tag="VFF72_06390"
     CDS             complement(207..497)
                     /locus_tag="VFF72_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92828.1"
                     /translation="MAKEKSIFEKARAAVAGMFSDEKKPASRKSPVRVAAGKKAAATT
                     ATKKAVVATRKAIVATKKAARTSTTRIASATKTAKKKVTAKKSATRKTPARK"
     gene            807..1172
                     /locus_tag="VFF72_06395"
     CDS             807..1172
                     /locus_tag="VFF72_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92829.1"
                     /translation="MMHGRNIRENLPSNGLRHGLTLAAMVAATLAAMLSACATSDGVV
                     PMADGTYTVTAEANFGPNKAVEAHKLALAEAERTCSAQGRELAVTTLDNSGDIGRAAG
                     PLALRGETRLVFRCVARRP"
     gene            complement(1211..1864)
                     /locus_tag="VFF72_06400"
     CDS             complement(1211..1864)
                     /locus_tag="VFF72_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZV92830.1"
                     /translation="MSGSGPSTFWHLVTRLGEAQIVLPAAILTALALFRRPQGRSLSV
                     AWMGWLFVAVVLTTATKVAFIGWGVGWAALDFTGISGHAMFSSSIYPVLLGASFRPMS
                     RRGWWIGVAIGVALALLIGVSRVVVGAHSVSEVVAGLLLGFAVSAASMRHDAIGLGFL
                     HPLLPVVIALWLVLTPTQASASRTHSAVTRLSLLLSGHARPFTRRDLLRRLHPRPTA"
     gene            complement(2330..>3100)
                     /locus_tag="VFF72_06405"
     CDS             complement(2330..>3100)
                     /locus_tag="VFF72_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92831.1"
                     /translation="GVSSSTLGAGTIVWILFVALVASGAGGYLAGRLRTKWVAVHTDE
                     VYFRDTAHGFLAWAVATLVTAATLTTAVGSIVGMGVDAAGTAAGGAGAATALAAGRGS
                     LASGQAGNAERGMQAMPGLAYYTDSLFRAAPPAGAAASTPSGSMAAQPVPSAQINRIF
                     VRSFENQGMAPADTQYLAQVVAQRTGLSQADAEKLVTTTYANASTAAQEAQTKAREAA
                     DKTRKAAAYAALWLFVSLLLGAFSASLFATLGGRQRDL"
BASE COUNT          536 a         1090 c         1032 g          442 t
ORIGIN      
        1 gcgaattcag cgacgcaaat ccatgagcgg aatcgggtga tcccgactcg gtctgcgttg
       61 aacgggtggc ggccttgcga gcggggatga ctggcgccag agggtcgcat ttcgtgcccg
      121 ggcggagcgc gccgcttcga ccacgtcgcc cggccctgca acgaattccc cgcggccgtc
      181 gcctcgcaac ggctccccgc gacggcctac ttcctggccg gcgtcttcct tgtcgcggac
      241 ttcttcgctg tgactttctt cttcgcggtc ttcgtcgccg aagcgatccg cgtcgtcgat
      301 gtcctggccg ccttcttcgt cgcgacgatc gccttccttg tcgcgacgac ggccttcttc
      361 gtcgcggtcg tcgcggcggc cttcttgccg gccgccacac gcacgggcga cttgcggctc
      421 gccggtttct tttcatcgct gaacatcccg gcgaccgcgg cgcgtgcctt ttcgaagatg
      481 cttttttcct tggccattga tcgatcccgg ttgatgagtc gcgcaagtgt gccaagcgac
      541 gcccgtgccg cgcaagaact atccgccctc gcgtcgcgcc gaggctgcag gtcgccctcg
      601 gtcggccgcg ccacgacgct gcgcgcggcc atgaccccgg cacgccgagt caaccgcgga
      661 tggcgcggct cgaaccagca aggcacgcca acgcaacttt gcatgaaggc gtgtcggcga
      721 aggcaggcga cgaagcgttg ggcagttgtc ctacgaagct cagcgtcgcc atcgacgatc
      781 ttcggcccat gccccaggag cagacgatga tgcacggaag aaacattcgc gaaaacctcc
      841 cttcgaacgg gcttcgccat ggcctgaccc tcgcggcgat ggtcgcggca accctggcgg
      901 cgatgctctc ggcgtgcgcc accagcgacg gcgtggtgcc gatggccgac ggcacctaca
      961 ccgtcacggc cgaggcgaac ttcggtccta acaaggcggt cgaggcccac aagctcgccc
     1021 tcgcggaagc agagcgaacc tgctccgcgc agggtcgcga gctggccgtc acgacgctcg
     1081 acaacagcgg cgacatcggc cgcgccgccg ggccgctcgc ccttcgcggc gaaacgcgcc
     1141 tggtctttcg ctgcgtcgca cgacgaccct gacctgccag cagcgcggcg cgtccgcgcg
     1201 cgtcgcgtgc ctaggccgtc gggcgcggat gcaatcgccg cagcaggtcg cgtcgggtga
     1261 acggcctggc gtggcccgag agcaggagcg acagccgcgt caccgccgaa tgcgttcgcg
     1321 acgccgacgc ctgcgtcggc gtgagcacca gccacagcgc gatgacgacc ggaagcagcg
     1381 gatgcagaaa tcccagcccg atcgcgtcgt gtcgcatcga ggcggcgctg accgcgaagc
     1441 ccagcaggag gcctgccacc acctccgaca ccgaatgcgc gccgacgacg acccgcgata
     1501 caccgatcag cagcgcgagg gcgaccccga tcgccacgcc gatccaccat ccccggcgag
     1561 acatcggcct gaacgacgcg ccgagaagaa cggggtagat ggacgacgag aacatggcat
     1621 gaccggagat gccggtgaaa tcgagcgccg cccagccgac gccccagccg atgaacgcga
     1681 ccttggtcgc agtcgtcagc acgacggcca cgaagagcca tcccatccag gcgaccgaga
     1741 gcgaccggcc ttgcggtcga cggaaaagcg ccagcgccgt caggatggcc gccggcagga
     1801 cgatctgcgc ttcgcccagg cgagtgacga gatgccagaa ggtcgaggga ccggatccgc
     1861 tcatggcgac ttcacggaac tcgccgaccc gccgggaccg ccgaccgcgg acgttccccg
     1921 cagtgacggc cgggacgaaa gcgttggcgg tgcgagccgg cccggtgcgt ttccctcaag
     1981 gaaaggcccc tgtgagttcg cgactcgcaa gggcccttcc aaggggctgg cacagacgtg
     2041 gcgtcaagaa cggctgccga cgacgaccct cgaatccgct cgatttgaat tcagccatcg
     2101 gcccaagcgt acgccctcga ccgacgccgg gaacgatccg gtcgcggtcg tctgcaccgg
     2161 gcggcgtccg gccacggttg aagtgccgcg agccgggctc gaaacgccgc aggccggacc
     2221 gaaccctttc gactcagtgc atgaacagcg cgatcaagat gatgatcgga atcggcacgc
     2281 cgagaaacca gagaaggatc gaacgcatgg gaatgctcct tgatgggaat cagagatcgc
     2341 gctggcggcc gcccagcgtc gcgaacaggc tcgcactgaa ggcaccgagc agaagcgaga
     2401 cgaacagcca caacgccgca taggccgctg ccttgcgggt cttgtcggcc gcttcgcggg
     2461 ccttggtctg cgcctcctgg gcggcggtgc tcgcattggc atacgtcgtg gtgacgagct
     2521 tttccgcgtc ggcctgcgac aggcccgtgc gctgcgcgac gacctgcgcg aggtattgcg
     2581 tgtcggcggg agccatgccc tggttctcga aactgcgcac gaagatcctg ttgatctgcg
     2641 ccgacggaac cggctgcgcc gccatcgacc cggacggcgt agaagcggca gcgccggcgg
     2701 gaggagcggc acggaaaagc gagtcggtgt agtacgccag acccggcatc gcctgcatcc
     2761 cgcgttcggc gttgcccgcc tggcccgatg cgagcgaccc gcgacctgca gccagggctg
     2821 tcgccgctcc tgcgcctccg gccgccgtgc cggccgcgtc gacgcccatg ccgacgatcg
     2881 acccgacggc cgtcgtcagc gtcgcggccg tcaccagcgt ggcgacggcc caggccagga
     2941 agccatgcgc ggtatcgcgg aaatagacct cgtccgtatg cacggcgacc cacttggtgc
     3001 gaagtcgacc cgccaggtag ccgcccgcgc cggaagcgac gagggcgacg aaaaggatcc
     3061 agacgatcgt cccggcgccc agcgtcgacg aggacacgcc
//
LOCUS       DASRWS010000340         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_141448, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000340 DASRWS010000000
VERSION     DASRWS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141448"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFF72_06410"
     CDS             complement(<1..729)
                     /locus_tag="VFF72_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZV92832.1"
                     /translation="MTLRRERTDILILGSGGAGLFAALHAQQAAPELSITVAVKGLLG
                     KCGCTRMVQGGYNVALAEGDSVERHFMDTIEGSKWLADQDLAWTLVTTAVERIHELEN
                     ELGCFFDRNPDGTVHQKAFAGQTFDRTVHKGDLTGIEIIGRLAEQVWARGIGRLEEHR
                     AIELVRTADGSAIAGVLMIDMRSGEPVFVQAKSVLLATGGGPTMYKYHTPSGDKSCDG
                     MAMALRAGLALRDMEMVQFHPTGLL"
     gene            complement(726..1349)
                     /locus_tag="VFF72_06415"
     CDS             complement(726..1349)
                     /locus_tag="VFF72_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HZV92833.1"
                     /translation="MAHHELTTPVTEAQVRALRVNDTVTLQRTLYGIRDATQIHMFDR
                     GRKTRFDLNGHAVIHTAPNVKKVEPSERFPAGYEAVCIGTTTSDRMERFTRPLMAQCG
                     VRLVIGKGGLRDASAQAFRELGGAYLAIIGGTAALETTWIEQIEDVDLDDLNPESLWK
                     FRIRDFGPLLVGMDSHGGSLYSVVRDAAAARRADVLKSLGVAAGSRA"
     gene            complement(1364..2239)
                     /locus_tag="VFF72_06420"
     CDS             complement(1364..2239)
                     /locus_tag="VFF72_06420"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZV92834.1"
                     /translation="MQVDLGAVEETAKELYIRALKLLPPDIKSGFERLQANETAPTAK
                     SVLGTMVTNIRVAEDTDNLLCQDTGVPIYNVWIGRGVEVDGHALKEAVRKGCERATRE
                     YPLRSSVVHPLTRKNEHTSCGIEVPVIHIDFRAEPDLLEIEMVPKGSGSENNSFLKMA
                     IPAEGVDAIKTFVIDCVLDAGGKTCPPTIVGVGLGGTSDLAVALAKRAATRPLGSRCA
                     DPDGAALEAELSAAVNRLGVGPQGLGGDATSFAVHIELAATHITMNPIAVNMQCHSAR
                     RARARFTPQGVAYGF"
     gene            complement(2259..2612)
                     /gene="sdhC"
                     /locus_tag="VFF72_06425"
     CDS             complement(2259..2612)
                     /gene="sdhC"
                     /locus_tag="VFF72_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZV92835.1"
                     /translation="MKRNDFRQRAHPAYWAFLVHRLSGLALAAFLPLHFWALGRALRG
                     EAALEGFLRFTDRPLFKLAEWGLVVLLALHLMGGTRLLLIEFGAASGLRKNWIAIAVA
                     VAAAVGLAFALALVT"
     gene            complement(2609..3022)
                     /locus_tag="VFF72_06430"
     CDS             complement(2609..3022)
                     /locus_tag="VFF72_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HZV92836.1"
                     /translation="MSDAEPLPAGPGRVEAWHGDGGDAASGVAEARRWYWQRVSAMVL
                     GVVVVVHLATVVYAVRGGLSAAEILGRTRGSWTFGVFYAVFVVACAVHVPIGLANIAR
                     EWWSLDERAALWLGRAFALVLLVMGLAAVAGVVLP"
     gene            complement(3019..>3730)
                     /locus_tag="VFF72_06435"
     CDS             complement(3019..>3730)
                     /locus_tag="VFF72_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV92837.1"
                     /translation="TLKVSVWRGREAGAFARYDVPRRDSQTVLDVVTHIQRHLEPGLA
                     YRFACRVGMCGSCAMTVNGKACWTCRTHVAHVAGEGGEIEIGPLANLPVVKDLVTDMR
                     TFFDKWARARGRFTGAATRADAFARVEPASPARREADAGIECIGCGVCYASCDVVAWR
                     PDYLGPAALNRAWTLVNDERDVAQKDRLRAVAGDAGCHACHTQGSCTERCPKHIAPTL
                     SIAGLKRTTARAAARGRL"
BASE COUNT          555 a         1291 c         1334 g          550 t
ORIGIN      
        1 gaggagcccc gtgggatgga actgcaccat ctccatgtcg cgcagcgcga gaccggcgcg
       61 gagcgccatc gccatgccgt cgcaggattt gtcacccgag ggggtgtggt acttgtacat
      121 cgtcgggccg ccgccggtgg cgagcagcac cgacttcgcc tgcacgaaca cgggctcgcc
      181 gctgcgcatg tcgatcatca gcacgccggc gatcgcgctg ccgtcggcgg tgcggaccag
      241 ctcgatcgcc cggtgctcct cgaggcggcc gatgccgcgc gcccagacct gctcggccag
      301 gcggccgatg atctcgatgc cagtcaggtc gcccttgtgg acggtgcggt cgaacgtctg
      361 gccggcgaat gccttctggt ggacggtgcc gtcggggttg cggtcgaaga agcagccaag
      421 ctcgttctcg agctcgtgga tgcgctcgac cgcggtcgtc acgagcgtcc acgccaggtc
      481 ctggtcggcg agccacttgc tgccctcgat cgtgtccatg aagtggcgct cgaccgaatc
      541 gccttcggcg agcgccacgt tgtagccgcc ctggaccatg cgggtgcagc cgcacttgcc
      601 gagcaggccc ttgacggcga cggtgatcga gagctcgggc gcggcctgct gcgcgtgcag
      661 cgccgcgaag aggccggcac cgcccgaccc caggatgagg atgtcggtgc gctcgcggcg
      721 cagcgtcatg cgcgcgagcc cgcggcgacg ccgaggcttt tcagcacgtc ggcgcgccgc
      781 gccgcggcgg cgtcgcgaac gacgctgtag aggctgccgc catggctgtc catgccgacg
      841 agcaaggggc cgaaatcgcg gatccggaac ttccagagcg actcgggatt gaggtcgtcg
      901 agatcgacgt cctcgatctg ctcgatccac gtcgtctcga gcgccgcggt gccgccgatg
      961 atcgcgagat acgcgccgcc gagctcgcga aaggcctggg cggaggcgtc gcgcaggccg
     1021 cccttgccga tcacgagacg caccccgcac tgcgccatca gcggtcgcgt gaagcgctcc
     1081 atccggtcgg acgtggtcgt gccgatgcac accgcctcgt agccggccgg gaagcgctcg
     1141 ctcggctcga ccttcttgac gttgggcgcg gtgtggatca cggcgtggcc gttcaggtcg
     1201 aaacgggtct tgcggccgcg atcgaacatg tggatctgcg tggcgtcgcg gatgccgtag
     1261 agcgtgcgct gcaaggtcac cgtgtcgttg acgcgcaatg cgcgcacctg cgcctcggtg
     1321 acgggtgtcg tgagttcgtg atgggccatc gccgtgtctc cggtcagaag ccgtacgcga
     1381 cgccttgcgg cgtgaagcgg gcgcgggcgc ggcgtgccga gtggcactgc atgttgaccg
     1441 cgatcgggtt catcgtgatg tgcgtcgccg cgagctcgat gtgcaccgcg aaagaggtcg
     1501 catcgccgcc gagcccctgc gggccgacac cgagacggtt gaccgccgcg gaaagctcgg
     1561 cttcgagcgc cgccccgtcg ggatcggcgc agcggctgcc gagcggacgc gtcgccgcgc
     1621 gtttggcgag cgcgaccgcg aggtcggaag tgccgccgag cccgacgccg acgatcgtcg
     1681 gcgggcacgt cttgccgccg gcatcgagca cgcagtcgat cacgaaggtc ttgatcgcat
     1741 cgacgccctc ggccgggatc gccatcttca ggaacgagtt gttctccgag ccgctgccct
     1801 tgggcaccat ctcgatctcg agcaggtcgg gctcggcgcg aaagtcgatg tggatcaccg
     1861 ggacctcgat gccgcacgag gtgtgctcgt tctttcgcgt cagcgggtgc acgaccgagc
     1921 tgcgcagcgg gtattcgcgg gtggcacgct cgcagccctt gcgcaccgct tccttcagcg
     1981 cgtgtccgtc gacctcgacg ccgcgtccga tccagacgtt gtagatcggc acgccggtgt
     2041 cctggcagag caggttgtcg gtgtcttcgg cgacgcggat gttcgtcacc atggtgccga
     2101 gcacgctctt ggccgtcggc gccgtctcgt tggcctgcag gcgctcgaag ccgctcttga
     2161 tgtcgggcgg cagcagcttg agcgcgcgga tgtagagctc cttggcggtc tcctcgaccg
     2221 cgccgagatc gacttgcatc gtctgttctc cgatgtgttc aggtgacgag ggcgagcgcg
     2281 aaggcgaggc ccacggcagc cgccaccgcg accgcgatcg caatccagtt cttgcgcaat
     2341 cccgaagcgg cgccgaactc gatcagcaac aggcgcgtcc cgcccatcag gtgcagcgcc
     2401 agcagcacga cgaggcccca ttcggcgagc ttgaagagcg ggcggtcggt gaagcgcagg
     2461 aagccctcga gcgccgcctc gccgcgcagt gcccgaccga gagcccagaa gtgcaagggc
     2521 aggaacgcgg ccagcgcgag cccggacaag cggtgcacga ggaaggccca gtaagccggg
     2581 tgcgcgcgct ggcgaaagtc gttgcgcttc aaggcagcac cactccagcg acggcggcca
     2641 ggcccatcac gagcagcacc agcgcgaagg cgcggccgag ccagagggcg gcgcgctcgt
     2701 cgagcgacca ccactcgcgc gcgatgttcg ccaggccgat cggcacgtgc acggcgcagg
     2761 cgacgacgaa caccgcgtag aagacgccga acgtccagct gccgcgcgtg cgacccagga
     2821 tctcggcggc gctgaggccg ccacggaccg cgtagacgac cgtcgcgagg tgcacgacga
     2881 cgacgacccc gagcaccatg gcgctgacgc gctgccagta ccagcgccgt gcttcggcca
     2941 caccggacgc ggcgtcgccg ccgtcgccgt gccaggcttc gacgcggccc gggcctgccg
     3001 gcagcggctc ggcgtcgctc acaaccgtcc ccgcgcggcg gcccttgcgg tggtccgctt
     3061 cagcccggcg atcgaaagcg tcggcgcgat gtgcttcggg cagcgctcgg tgcacgaccc
     3121 ctgggtatgg cacgcatggc agccggcatc gccggcgacg gcccgcagcc gatccttctg
     3181 cgccacgtcg cgctcgtcgt tgacgagcgt ccaggcccgg ttcagtgccg ccggcccgag
     3241 gtagtcgggc cgccaggcga cgacgtcgca cgaggcgtag cagacgccgc agccgatgca
     3301 ctcgatgccg gcgtcggcct cgcggcgcgc cggcgacgcc ggctcgacgc gggcgaacgc
     3361 gtcggcgcgc gttgccgcgc cggtgaagcg tcccctggcg cgcgcccact tgtcgaagaa
     3421 cgtgcgcatg tcggtcacga gatccttgac gaccggcagg ttcgcgagcg gcccgatctc
     3481 gatctcgccg ccctctccgg cgacatgggc gacgtgggtg cggcaggtcc agcaggcctt
     3541 gccgttgacc gtcatcgcgc acgagccgca catcccgacg cggcaagcga agcgataggc
     3601 gagcccgggc tcgagatggc gctggatgtg cgtgacgaca tcgagcaccg tctggctgtc
     3661 gcggcgcggc acgtcgtagc gcgcgaaggc gccggcctcg cggccacgcc agacactcac
     3721 cttcaaggtg
//
LOCUS       DASRWS010000341         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_141713, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000341 DASRWS010000000
VERSION     DASRWS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141713"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFF72_06440"
     CDS             complement(<1..632)
                     /locus_tag="VFF72_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZV92838.1"
                     /translation="MTTAASACPTVDEIAALFTPRSHWQSWLEVESALAETQAELGMI
                     PAEAAAEIVRKASFEYIDATALGADIARSRAPIVSLVRALAAACDGDAGGYVHWGATT
                     QNVMQTARVLLMRRAHVAFTARLAEVLLALAGLAEEGADMLVAARTNFRQALPITFGF
                     KAAAWIEEMLRHVQRFEGAAPRVFAALWGGAVGAMHAFGDHGVELNQRLSA"
     gene            complement(632..1366)
                     /locus_tag="VFF72_06445"
     CDS             complement(632..1366)
                     /locus_tag="VFF72_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZV92839.1"
                     /translation="MPGTPVRFENIVKSFGRVEVLRDITLDVPGGVTTCIVGPSGSGK
                     STLLRCANLLERPQSGRILLGGADILAAGVDIDAVRARVGMVFQHFHLFSHMSVLDNV
                     AIALRKVRGQGRDEAETAARAKLADVGLAGFESRRPSALSGGQQQRVAIARALAMEPE
                     VMLFDEATSALDPELVKGVLAVMRDLAGGGMTMIVVTHEMRFAREVAGQVVFIDQGRI
                     VEHAPPDAFFDHPQHPRLRHFLEQVL"
     gene            complement(1372..2037)
                     /locus_tag="VFF72_06450"
     CDS             complement(1372..2037)
                     /locus_tag="VFF72_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV92840.1"
                     /translation="MPEVLTVGLPNTLLLSAFASVIGIVLGLLVASGLLSGHRGIRLP
                     CRGYVDILRGLPHILTVYLIGQGLPLAGLSLFGDDTYGYAALAIGLIESAYFAEIFRA
                     GFQSVERGQVEAARSTGMSRLQTMRYIIVPQGARRVLPPLTGQFILVIKATSLVYLLG
                     LTASQREMFAVAQDAAINSANLSPLTAAGLVYLALTIPTTYAINAWDRRLRGTGAPRP
                     IEG"
BASE COUNT          328 a          732 c          765 g          284 t
ORIGIN      
        1 gccgagaggc gctggttgag ctcgacgccg tggtcgccga aggcgtgcat cgcaccgacg
       61 gcgccgcccc agagcgccgc gaagacccgc ggcgcggcgc cttcgaaccg ctgcacgtgg
      121 cgcagcatct cctcgatcca cgccgccgcc ttgaagccga aggtgatcgg cagcgcctgc
      181 cggaagttgg tgcgggccgc caccagcatg tcggcgcctt cctcggccag gccggcgagc
      241 gcgagcagca cctcggcgag tcgggccgtg aaggcgacgt gggcacggcg catgagaagg
      301 acgcgcgccg tctgcatgac gttttgcgtc gtcgcgcccc agtgcacgta gccgccggcg
      361 tcgccgtcgc aagccgcggc gagcgcgcgc accagcgaga cgatcggcgc ccggctgcgg
      421 gcgatgtccg cgccgagggc cgtcgcgtcg atgtactcga agctcgcctt gcgaacgatc
      481 tccgccgccg cctcggccgg gatcatgccg agctcggcct gcgtctcggc cagcgccgac
      541 tcgacctcga gccacgactg ccagtggctg cgcggcgtga agagcgccgc gatctcgtcg
      601 accgtcgggc acgcggatgc cgccgtcgtc atcacagcac ctgctcgagg aagtgacgca
      661 ggcgcggatg ctgcggatgg tcgaagaacg cgtcgggcgg cgcgtgctcg acgatccgtc
      721 cctggtcgat gaagacgacc tgcccggcca cctcgcgcgc gaagcgcatc tcgtgggtga
      781 cgacgatcat cgtcatgccg ccgccggcga ggtcccgcat caccgccagc acgcccttga
      841 cgagctcggg gtcgagcgcc gaggtcgcct cgtcgaaaag catgacctcg ggctccatcg
      901 ccagcgcccg ggcgatcgcc acgcgttgct gctggcctcc ggaaagcgcc gacggccggc
      961 gcgactcgaa gccggccagg ccgacgtcgg cgagcttcgc gcgcgccgcc gtctcggctt
     1021 cgtcgcgccc ctggccgcgc accttgcgca gggcgatggc gacgttgtcc agcacgctca
     1081 tgtgcgagaa cagatggaag tgctggaaca ccatgccgac gcgcgcccgc acggcgtcga
     1141 tgtcgactcc cgcggccagg atgtcggccc cgccgagcag gatgcgcccc gattgcggcc
     1201 gctcgagcag gttggcgcag cgaaggagcg tcgactttcc cgagcccgac ggaccgacga
     1261 tgcaggtcgt cacgccgccg ggaacgtcga gcgtgatgtc cctcaggacc tcgacacgtc
     1321 cgaacgactt gacgatgttc tcgaagcgga cgggcgttcc cggcatcgcc gtcacccttc
     1381 gatcggccgc ggcgcgccgg tgccgcgcag gcgccggtcc caggcgttga tcgcgtaggt
     1441 cgtgggaatc gtcagggcga ggtagacgag cccggcggcg gtgagcggcg acaggttggc
     1501 gctgttgatc gcggcatcct gggcgacggc gaacatctcc cgctgcgacg cggtcaggcc
     1561 gagcaggtag acgagcgacg tcgccttgat gacgaggatg aactggccgg tgagcggcgg
     1621 cagcacacgg cgcgcgcctt gcggcacgat gatgtagcgc atcgtctgca ggcgcgacat
     1681 gccggtgctg cgcgccgcct cgacctggcc gcgctcgacg ctctggaagc cggcgcggaa
     1741 gatctcggcg aaataggcgc tctcgatcag gccgatggcg agcgccgcat agccgtaggt
     1801 gtcgtcgccg aacaggctga gtccggccag cggcaggccc tgtccgatca ggtagacggt
     1861 gagaatgtgc ggcaggccgc gcaggatgtc gacgtagccc cggcacggca ggcggatgcc
     1921 gcggtgcccc gacaggagtc ccgaggcgac gagcagcccg agcacgatgc cgatcacgct
     1981 cgcgaacgcc gacaacagca gcgtgttcgg cagcccgacg gtgagcacct cgggcatcgc
     2041 ctgcgcgatc agcgtcgggt cgaagaaggt ggcgaggatc cggtgcagtc actcgggcat
     2101 ggtcagtcc
//
LOCUS       DASRWS010000342         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3320125, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000342 DASRWS010000000
VERSION     DASRWS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320125"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFF72_06455"
     CDS             complement(<1..343)
                     /locus_tag="VFF72_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZV92841.1"
                     /translation="MTWTEVRDAIAAGRTTVIVPVGGTEQSGPHIALGKHNVRARLLA
                     GRIARALGNALVAPVVAYVPEGSIDPPSGHMRFPGTISVSDAAFRGVLEGAARSLRRA
                     GFRDIVLIGDHG"
     gene            complement(532..1251)
                     /locus_tag="VFF72_06460"
     CDS             complement(532..1251)
                     /locus_tag="VFF72_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZV92842.1"
                     /translation="MTRSSSAAFLLARARAPHGQELADVVWLKALLPAERERALADLK
                     VAEVETGELLCRVGRPVTYWFGVIDGLLKTSNDSALGIPVTFTGLPPGGWFGEGTVIK
                     REAYRYNIQALRRSTVAGLPVETFHWLLDRSIPFNRFILQQLNERLGQFMAAREIDRL
                     ADADARVARSLAALFHPVLYPRVSALLRITQQELGYLVGLSRQRVNEALRRLEEAAAI
                     RVEYGGLRVLDLERLRGYGLR"
     gene            1293..1928
                     /locus_tag="VFF72_06465"
     CDS             1293..1928
                     /locus_tag="VFF72_06465"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV92843.1"
                     /translation="MTKQSFVFLIDDEMEAIRRRAHSRLPQLLDEAARAFAERGYAAT
                     SVRDIVGPIGMLPGSLYCHFETKEALLVAVYTEGVARISAAVDAAVARFGDPWTRLEA
                     ASIAHLRSLLDQTSYSQVVIRIRPADAPAVESELVVLRDRYEARWTHMVAALPLARGV
                     HRGDLRLFLLGALNWSQTWYRDDGASTPERIARRFVALMKASAGARSKETA"
     gene            1925..2371
                     /locus_tag="VFF72_06470"
     CDS             1925..2371
                     /locus_tag="VFF72_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZV92844.1"
                     /translation="MTSEFRPRILVTKIGLDGHDRGSRIVAAACRDAGMEVIYTPPWQ
                     SIPAVVKLTLEEDVDVVGISSLATDHLIVPKMMQALGEAGLGHVVVVVGGIVPASEEA
                     MLVASGVREVFHPGSTMDEITTRIRAYAAEARRTGQAAAAGQEATP"
     gene            2368..>3624
                     /locus_tag="VFF72_06475"
     CDS             2368..>3624
                     /locus_tag="VFF72_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HZV92845.1"
                     /translation="MNKPLDSPLVSATESAAKADWKREYQATIGHDRDIANVSGIPIQ
                     PLYGADDWAAYGRPEPLGYPGQPDYTRGIYATMHRGRTWTQRQLIGLGRPDDYNARLR
                     EIMARGATAVSLIPCNSVYRGYDMGSVEPELLGTCGVVVNNAPHMARCLDGVDVGEIS
                     CAMNDPSPFTLLAFMLVTAERRGTSWQKIAGTSNQSDYLSHYVANHMFYRLSLPGSRR
                     VLMDHITWCNEHLPKWNPMSVVGQHMQQAGATPAEAMAFTLCTAIQNAEDCIGRGMDP
                     DHFLPRFTFFFDISMSFFEEVAKFRAGRRIWARLARERLGAKDPKSWRFKFHGQTSGV
                     DLTRQQPLNNIARVTAQAMAGIFGGLQSLHTDSWDEVFQVPGEAAARIAVATQNILRE
                     EAHLTDVIDPLGGSFYVESLTDAMEER"
BASE COUNT          591 a         1288 c         1224 g          521 t
ORIGIN      
        1 cgccgtggtc gccgatcagg acgatgtcgc gaaagccggc gcgccgaagg ctgcgtgccg
       61 cgccttccag gacaccgcga aaagccgcat cggacacgga gatcgtcccg ggaaaccgca
      121 tgtggccgct cggcgggtcg atgcttccct cgggcacgta ggcgaccacc ggcgcgacca
      181 gggcgttgcc gagcgcgcga gcgatccgcc ctgcgagcag gcgggcgcgc acgttgtgct
      241 tgccgagggc gatgtgcggc ccgctctgct cggtgcctcc gaccggaacg atgaccgtgg
      301 tacgaccggc ggcgatcgcg tcgcgcacct cggtccacgt catgtcggcc aggtagacat
      361 cccgatcggc ggtgaccggt ccggtcggcg ccgcgcggac gagcgtcgac acgaggagga
      421 agcagagcgt caggacgatg cggtgcatga aaggaaggaa gcgccggagt gcgatgttag
      481 gacgcaatcg ccggcgagaa cgcgggtgcg ccgagcgctt ggccgcaacc ctcagcgcag
      541 gccgtagccg cgcaggcgct cgaggtcgag gacgcgcagg ccgccgtact cgacgcggat
      601 cgcagcggcc tcctcgagtc gacgaagcgc ttcattcacg cgctgccgcg acaggccgac
      661 gagatagccc agctcctgct gcgtgatgcg cagcagcgcg ctgacccgcg ggtaaagcac
      721 ggggtggaac agcgccgcca ggctgcgcgc gacccgcgcg tcggcatcgg cgaggcggtc
      781 gatctcgcgc gccgccatga actggccgag tcgctcgttg agctgctgga ggatgaagcg
      841 gttgaacggg atgctgcggt cgaggagcca atggaaggtc tcgaccggca gcccggccac
      901 cgtgctgcgt cgcaacgcct ggatgttgta tcgataggcc tcgcgcttga tcaccgtgcc
      961 ctcgccgaac cagccgcccg gcggcaggcc ggtgaaggtg acgggaatgc ccagcgccga
     1021 gtcgttgctc gtcttcagca agccgtcgat gacgccgaac cagtacgtca cgggtctgcc
     1081 gacgcggcag agcaactcgc cggtctcgac ctcggcgacc ttcaggtcgg ccagcgcgcg
     1141 ctcgcgctct gcgggcagca gcgccttcag ccagacgacg tcggccagct cctgcccgtg
     1201 cggcgcgcgg gcgcgggcaa gcaggaaggc ggctgacgag ctgcgagtca cgagcgccat
     1261 tgtcgatccg gcgccggcga cgatcgcgcc acttgaccaa acaatcgttt gtctttctaa
     1321 tcgacgacga gatggaagcg attcgtcgac gcgcgcacag ccgcctgccc cagctgctcg
     1381 acgaagcggc gcgcgccttc gccgagcgcg gctacgccgc cacctcggtg cgcgacatcg
     1441 tcggcccgat cggcatgctg ccgggctcgc tctactgcca cttcgagacc aaggaggcgc
     1501 tgctcgtcgc cgtctacacc gaaggcgtcg cccgcatctc ggcggcggtg gacgccgccg
     1561 tggcacgctt cggcgatccg tggacccgcc tcgaagcggc gtccatcgcc caccttcgct
     1621 cgctgctcga ccagaccagc tattcgcagg tggtgatccg catccggcca gccgatgccc
     1681 cggcggtcga atccgagctg gtcgtcctgc gcgaccgcta cgaagcgcgc tggacgcaca
     1741 tggtggcggc gttgccgctg gcgcgtggcg tgcaccgcgg cgatctgcgc ctgttccttc
     1801 tcggcgcgct caactggagc cagacctggt atcgcgacga cggcgcctcg acgcccgaac
     1861 ggatcgcccg ccgcttcgtc gccctcatga aggcgagcgc cggggcgcgc agcaaggaaa
     1921 ccgcatgacc agtgaatttc gcccacgcat cctcgtcacg aagatcggcc tcgacggtca
     1981 cgaccggggc agccgcatcg tcgccgccgc ctgccgcgac gccggcatgg aagtgatcta
     2041 cacgccgccg tggcagagca ttccggcggt cgtcaagctg acgctcgagg aggacgtcga
     2101 cgtcgtcggc atctcgtcgc tcgccaccga ccacctcatc gtcccgaaga tgatgcaggc
     2161 gctcggcgag gccggcctcg gccatgtggt ggtggtggtc ggcggcatcg tccccgccag
     2221 cgaggaagcg atgctggtcg ccagcggcgt gcgcgaggtc ttccatccgg gctcgacgat
     2281 ggacgagatc acgacgcgca tccgcgccta tgccgccgag gcccgccgca ccggccaggc
     2341 ggcggccgcc gggcaggaag cgacaccatg aacaagccgc tcgactcccc gctcgtgtcc
     2401 gccaccgaga gcgctgcgaa ggccgactgg aagcgcgagt accaggcgac gatcggccac
     2461 gatcgcgaca tcgccaacgt cagcggcatc ccgatccagc cgctctacgg cgccgacgac
     2521 tgggccgcct acggtcggcc cgaaccgctc ggctacccgg gccagcccga ctacacgcgc
     2581 ggcatctacg ccaccatgca ccgcggccgc acctggacgc agcgccagct gatcggcctg
     2641 ggcaggcccg acgactacaa cgcgcgcctg cgcgagatca tggcccgcgg cgccaccgcc
     2701 gtctcgctga ttccgtgcaa ctcggtctat cgcggctacg acatgggttc ggtcgagccg
     2761 gagctgctcg gcacctgcgg cgtcgtcgtc aacaacgccc cgcacatggc gcgctgcctc
     2821 gacggcgtcg acgtcggcga gatctcgtgc gcgatgaacg atccgtcgcc gttcacattg
     2881 ctggccttca tgctcgtcac cgccgagcgc cgcggcacgt cgtggcagaa gatcgccggc
     2941 acctcgaacc agagcgacta cctgtcgcac tacgtcgcca accacatgtt ctaccggctg
     3001 tcgctgcccg gttcgcggcg cgtgctgatg gatcacatca cgtggtgcaa cgagcatctg
     3061 ccgaagtgga acccgatgtc ggtcgtcggc cagcacatgc aacaggcggg agcgacgccc
     3121 gccgaggcga tggccttcac gctctgcacc gcgatccaga acgcagagga ctgcattggc
     3181 cgcggcatgg atcccgacca cttcctgccg cgcttcacct tcttcttcga catctcgatg
     3241 agctttttcg aggaggtggc gaagttccgc gccggccgtc gcatctgggc gcgcctggcc
     3301 cgcgagcgcc tcggcgcgaa ggatccgaag tcgtggcgct tcaagttcca cggccagacc
     3361 tcgggcgtcg acctgacgcg tcagcaaccg ctcaacaaca tcgcccgcgt caccgcgcag
     3421 gcgatggccg gcatcttcgg cggcctgcaa tcgctgcaca ccgattcctg ggacgaggtc
     3481 ttccaggtgc cgggcgaagc ggcggcgcgg atcgccgtgg cgacgcagaa catcctgcgc
     3541 gaggaagcgc acctcaccga cgtgatcgac ccgctcggcg gctcgttcta cgtcgagtcg
     3601 ctgaccgacg cgatggaaga aagg
//
LOCUS       DASRWS010000343         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1005509, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000343 DASRWS010000000
VERSION     DASRWS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005509"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFF72_06480"
     CDS             <1..403
                     /locus_tag="VFF72_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV92846.1"
                     /translation="EWYWNQPNVPHVAEFVADIRKKTNGHVPTARTWFGYATAWTCAL
                     AAQNAKSLEAVKMAKALQGFKLPPEVALMPDGAYYRAAQNQLIPNLYVGNAQAKGEKD
                     AEDLFKVTEVAKGADVAGTLEETGCKMTWPT"
     gene            558..1424
                     /locus_tag="VFF72_06485"
     CDS             558..1424
                     /locus_tag="VFF72_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92847.1"
                     /translation="MTQLVLFNVFNGLIVGAFYALMALGLSLILNLSGVINFAHGGFL
                     AIGGYVAYALIPYVGFWGALLLAPFVTAVLGLIVERLLIRRLYGRDPLYSLLLTFGLA
                     FMFEDGTRFIWGAQSLPYQVPGWLLSPLSSAYFFLTGYRLFMVAIVAIAVGGLFLILT
                     YTRLGVRIRAGTLDLETVSVLGVNVRWLRSLNFGLGVYLAGLAGVLAVGQLGLQPTIG
                     SSLIMPSFVAIIVGGLGSLPGTLLGGLLIGVASGVTTVFFPSATEAVMYVMMAAVLMV
                     RPRGLLGEEGRL"
     gene            1436..2380
                     /locus_tag="VFF72_06490"
     CDS             1436..2380
                     /locus_tag="VFF72_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92848.1"
                     /translation="MTKQASNIVVWLLLATVPFWLPKVPGGYLELGSRALVLGLAAMA
                     LNFLLGYTGVLSFGHAAYFGLGAYGAGMTIRYLVPSTGLGLLIGTLVGTVAAALIGPL
                     IVRLRGVYFAMVTIAFGQVFYFIAFRWSNVTGGDDGLTNWTRQPLDLLVTRVDIEHSS
                     TAFYYLALVVFAVAVGVMAWLLRSPFGRTLIAIRENERRARFLGIPVDRHIWISWVIS
                     CAVVSVAGALYALLNNFVDPRALYWSQSGDFVIMAVLGGMRSFWGPLLGAAIFVVLQD
                     YLSSVTENWMSFVGLFFVLVVLFFPRGVLGMIRRRAAA"
BASE COUNT          334 a          802 c          821 g          503 t
ORIGIN      
        1 cgagtggtac tggaaccagc cgaacgtgcc gcacgtcgct gaattcgtcg ccgacattcg
       61 caagaagacg aacgggcatg tgccgacggc acgtacctgg ttcggctatg cgacggcgtg
      121 gacctgtgcc ctcgcggcac agaacgccaa gtcgctcgag gcggtcaaga tggcgaaggc
      181 gctgcagggc ttcaagctgc cgcccgaggt cgcgctgatg ccggacggcg cctactaccg
      241 cgcggcgcaa aaccagctga tcccgaatct ctacgtcggc aatgcgcagg cgaagggcga
      301 gaaggatgcc gaggacctgt tcaaggtgac cgaggtcgcc aagggcgccg acgtcgccgg
      361 cacgctggaa gagaccggct gcaagatgac ctggccgacc tgatcggcaa caagaccgcc
      421 gggcgcccac gcggcgcccg gcgtttccgg cgggggccgg cgtggggata cgatcgtctc
      481 agtgcaacga atggtgactt ggttcgcgcc ggcgcaagct gccgcgtgac cggcctacga
      541 tgacgatgta agcgcgcatg acacaactcg tcctattcaa cgtcttcaac ggcctgatcg
      601 tcggcgcctt ctacgcgttg atggcgctcg ggctgtcgct gatcctcaac ctgtccggtg
      661 tgatcaattt cgcccacggc gggttcctcg cgattggcgg ctacgtcgct tatgcgctga
      721 tcccttatgt cggcttctgg ggcgcgctgc ttctcgctcc gttcgtcacc gcggtcctgg
      781 ggctgatcgt cgagcgactt ctgatccgcc ggctctatgg gcgggatccg ctctacagcc
      841 tgctgctcac cttcggactt gccttcatgt tcgaggatgg cacgcgcttc atctggggcg
      901 cgcagagcct gccttaccag gtgccgggct ggctcctgtc gccgctgagc agcgcctact
      961 tcttcctcac gggctatcga ctgttcatgg tggcgatcgt cgccatcgcg gtgggcggcc
     1021 tctttctcat tctcacctac acccggctcg gcgtgcgcat ccgcgccggc acgctcgacc
     1081 tcgagacggt gtcggtgctc ggcgtcaatg ttcgctggct gcgcagcctg aacttcgggc
     1141 tcggcgtcta tctcgcgggc ctcgccggcg tgctcgcggt cggccaactc gggctgcagc
     1201 cgacgatcgg cagctcgctg atcatgccga gcttcgtcgc catcatcgtg ggcggattgg
     1261 gcagcctgcc cggaacgctg ctgggcggat tgctgatcgg ggtcgcgtcg ggtgtcacca
     1321 cggtcttctt cccgtcggcg accgaagccg tgatgtacgt catgatggcc gcagtgctca
     1381 tggtgcgccc gcgcggcctg ctgggtgagg aaggaagact gtagtgcgca gcgatctgac
     1441 caagcaggcg tcgaacatcg tcgtctggct gctgctggcg acggtgccgt tctggctgcc
     1501 gaaggtcccc ggcggctatc tggagctcgg cagccgcgcc ctggtgctcg ggcttgccgc
     1561 gatggcgctg aacttcctgc tcggctacac cggcgtgctg tcgttcggcc acgccgccta
     1621 tttcggcctc ggcgcctatg gcgccggcat gacgatccgt tacctcgtgc cgagcaccgg
     1681 tctgggtctc ctgatcggca cgctcgtcgg gacggtggca gccgcgctga tcgggccgtt
     1741 gatcgtgcga ctgcgcggcg tctacttcgc gatggtgacg atcgcgttcg gccaggtgtt
     1801 ctatttcatc gccttccgct ggagcaacgt gacgggcggt gacgacggcc tcaccaactg
     1861 gacgcgccag ccgctcgatc tcctggtcac gagggtcgac atcgagcaca gctcaaccgc
     1921 cttttactat ctcgcgctgg tcgtcttcgc cgtcgcggtc ggcgtgatgg cgtggctctt
     1981 gcgctcgccg ttcggccgca ccttgatcgc gatccgcgag aacgagcggc gcgcccgctt
     2041 cctcggcatc cccgtcgacc gccacatctg gatttcctgg gtgatctcct gcgccgtcgt
     2101 cagcgtcgcc ggcgcgctct atgcgctgct caacaatttc gtcgacccgc gcgccctcta
     2161 ctggagtcag tcgggcgatt tcgtcatcat ggcggtgctc ggcgggatgc gctcgttctg
     2221 gggaccgctg ctcggggcgg cgatcttcgt cgtgctgcag gactacctgt cgagcgtgac
     2281 cgagaactgg atgtcgttcg tcggattgtt cttcgtgctg gtggttctgt tcttcccgcg
     2341 cggcgtgctc ggcatgatcc gccggagggc tgcggcatga ccctgctgcg cgtcgagaac
     2401 gtgtcgaagc atttcggcag cctggtcgcg gtcgaccagg tgtcgatggc ggtcgagcgg
//
LOCUS       DASRWS010000344         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3592281, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000344 DASRWS010000000
VERSION     DASRWS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592281"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1512)
                     /locus_tag="VFF72_06495"
     CDS             complement(16..1512)
                     /locus_tag="VFF72_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HZV92849.1"
                     /translation="MPDPLPIARHGDLEIDLYPALANRHGLITGATGTGKTVTLQVIA
                     ERMSGIGVPVFMADVKGDLAGMSQPGAMSDKLAERLRTIGAPAPAFAAMPVVFWDIFG
                     DQGHPVRATVSDLGPLLLSRILNLNETQQGVLTLVFKLADDNGLLLLDMKDLRALLQY
                     AGENAASLTTAYGNVSAASIGAIQRGLLALEQQGGDRFLGEPMLNVDDLLQTDGSRGV
                     VNILAADRLMQSPQLYASMLLWLLSELYERLPEVGDRDKPKLVFFFDEAHLLFTDAPK
                     ALLDKIEQVVRLIRSKGAGVFFVTQNPLDVPDIVLGQLGNRVQHALRAFTPRDEKAVK
                     SAASTMRANPAIDTAKAITELGVGEALISFLDDKGRPGIVQRAFVLPPASRIGPITPD
                     ERKAVIDRSPVKGVYEKTVDRESAYEKLASRAGASASTPTIGAPSAGGGIAGSIADSL
                     GGLMKGSGRKDSLVEAMAKSAVRSVGSSIGRAIVRGVMGSLLGGRGRR"
     gene            1687..>2123
                     /locus_tag="VFF72_06500"
     CDS             1687..>2123
                     /locus_tag="VFF72_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV92850.1"
                     /translation="MAKVRAGSITMGYEQQGSGEPLILIPHLAADHACYAFQVADYAK
                     HFTCISVDLRDTGESDKSTGKYTTETLADDVAALMQALGIDKAHVSGLSLGAATGMWL
                     AAKYPGRVRSLSLHSAWSATDAYIGAVVGSWQVMAKALGSVIE"
BASE COUNT          333 a          718 c          724 g          348 t
ORIGIN      
        1 ccccggaacg cgagttcagc ggcggccgcg gccgccgagc agcgatccca tgacgccgcg
       61 cacgatcgcg cgcccgatcg acgagccgac gctgcgcacg gcgctcttcg ccatcgcttc
      121 gacgaggctg tccttgcggc cgcttccctt catgagaccg ccgagcgaat ccgcgatcga
      181 gccggcgatg cccccgccgg cgctcggtgc gccgatcgtc ggtgtcgatg cagaggcgcc
      241 ggcgcggctc gcgagcttct cgtacgccga ttcgcggtcg accgtcttct cgtagacgcc
      301 cttcaccggc gagcggtcga tcaccgcctt gcgctcgtcg ggcgtgatcg gcccgatccg
      361 cgaggccggt ggcagcacga atgcgcgctg cacgatgccg ggtcgtccct tgtcgtcgag
      421 aaacgagatc agcgcctcgc cgacgccgag ttccgtgatc gccttggcgg tgtcgatcgc
      481 aggattcgcg cgcatcgtcg acgcggcgga cttgaccgct ttctcgtcgc gcggcgtgaa
      541 cgcgcgcagc gcatgctgga cgcggttgcc gagctggccc agcacgatgt cgggaacgtc
      601 gagcggattc tgcgtcacga agaacacgcc ggcgcccttc gagcggatga gccgcacgac
      661 ctgctcgatc ttgtcgagca gtgccttcgg cgcgtcggtg aacaacaggt gcgcctcgtc
      721 gaagaagaac acgagcttcg gcttgtcgcg atcgccgact tcgggcagcc gctcgtagag
      781 ctccgacaag agccacagca gcatgctcgc gtagagctgc ggtgattgca tcaggcgatc
      841 ggcggcgagg atgttgacga cgccgcggct gccgtcggtt tgcagaaggt cgtcgacgtt
      901 cagcatcggt tcgccgagaa agcgatcgcc gccctgctgc tcgagcgcga gcaggccccg
      961 ctggatcgcg ccgatcgagg ccgccgagac gttgccgtac gccgtcgtca ggctcgcggc
     1021 gttctcgccg gcgtattgca gaagcgcgcg caggtccttc atgtcgagca acagcagccc
     1081 gttgtcgtcg gcgagcttga agaccagcgt cagcacgccc tgctgcgtct cgttcaggtt
     1141 caggatgcgg ctcaacagca aggggccgag gtcgctcacc gtcgcccgca ccgggtggcc
     1201 ctggtcgccg aagatgtccc agaacacgac gggcatcgca gcgaacgccg gcgccggcgc
     1261 gccgatcgtg cgcagacgct cggcaagctt gtcggacatc gcacccggct ggctcatgcc
     1321 ggccagatcg cccttgacgt cggccatgaa caccggaacg ccgatgccgc tcatgcgctc
     1381 ggcgatcacc tgcagcgtga cggtcttgcc ggtgcccgtc gcgcccgtga tcaatccgtg
     1441 gcggtttgcg agcgcgggat aaaggtcgat ttcgaggtcg ccgtgcctgg cgatcggcag
     1501 cgggtcgggc atcgtctggg tctcctcggc tgcaacggcg aaggcgcgag cgccgcgcgt
     1561 cgccgtggtc gcccaagttt agcgtgcgcc tgcgcatgca gcggcgtatg ctcgctcccc
     1621 aggcgccgga gcgactgaag tcgccgaact tcgggctccc gaacgcgccg ctcggcggag
     1681 gtcatcatgg ccaaggtacg cgccggatcc atcacgatgg gctacgagca acagggaagc
     1741 ggcgagccgc tgatcctgat cccgcatctt gccgccgacc acgcgtgcta tgcgttccag
     1801 gtcgccgact atgcgaaaca tttcacctgt atctcggtcg atcttcgcga taccggcgaa
     1861 tcggacaagt cgacgggcaa gtacacgacc gagacgctcg ccgacgacgt ggcggccctg
     1921 atgcaggcgc tcggcatcga caaggcgcac gtcagcggac tctcgcttgg cgccgcgacc
     1981 gggatgtggc ttgcggcgaa atatcccggg cgggtgcgct cgctctcact gcacagcgcg
     2041 tggtccgcga ccgacgctta catcggcgcg gtcgtcggca gttggcaggt gatggcgaaa
     2101 gcgctcggca gcgtgatcga gat
//
LOCUS       DASRWS010000345         5016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_448324, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000345 DASRWS010000000
VERSION     DASRWS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448324"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..427
                     /locus_tag="VFF72_06505"
     CDS             71..427
                     /locus_tag="VFF72_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZV92851.1"
                     /translation="MTPKTSMTTDLISSERVEGTSVYNSAGDKLGSIDDLVIDKRSGQ
                     VRYAVMEFGGFLGIDTDRYPIPWNMLRYDTDKDGYVVPLDKAKLENAPKYRDEDVPVY
                     DLTYGKRVNGYYGVDW"
     gene            complement(409..738)
                     /locus_tag="VFF72_06510"
     CDS             complement(409..738)
                     /locus_tag="VFF72_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92852.1"
                     /translation="MTGALFGQAPRKRRAVVWLDRQRVGFGLVGLRHSWKSGNREFSR
                     HSHRCSSQAEDEACRHHAGATHFIQQQEVPEAVQKGLWRDIAVRRSRLDNGAVAKSTL
                     EIYQSTP"
     gene            874..1329
                     /locus_tag="VFF72_06515"
     CDS             874..1329
                     /locus_tag="VFF72_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92853.1"
                     /translation="MILVLAVLGYGAWASDFITLQGERTVYTVGCQGGEWQGEVCSGK
                     LVAGDRYRYRALKPHHEVIFWKVGARDRSGKFDRCDIHDGRNWVCAPSDDASRSITLA
                     MDGGVPVPGPPTVTRPFRAVSKWRWILLRRGLSRKKVVPLIAAAPSPAH"
     gene            1444..1941
                     /locus_tag="VFF72_06520"
     CDS             1444..1941
                     /locus_tag="VFF72_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZV92854.1"
                     /translation="MKDPKIEERLGVACMVVLTVLTLGNVVTRYLTDESFAWTEEISV
                     FLLVVMTLAGAASIAGRDAHIRIEYFYDGGSDTRRRRLRLLAAASTALLFAGLAVLFG
                     IDLADEIRWNETSMGLGVPRWWFTAAIPPLCIAISARAVLAGRRALRGLPASPAAPFD
                     PEGRL"
     gene            1958..3256
                     /locus_tag="VFF72_06525"
     CDS             1958..3256
                     /locus_tag="VFF72_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZV92855.1"
                     /translation="MITATLVVLFVGLMLAGVPIGVALGIGGIVAIAASNPGSPAWGL
                     LAAPQNMHASVAKYPLLALPMFVLVGSIFDRSGVARRLVTFAQACVGHAPGMLPMVAI
                     LVAMLLGGISGSGPATAAAVGGVMIAAMNRAGYPPAFSASVVGAAAATDILIPPSIAF
                     IVYSVNVPGASVPALFAAGMVPGLLAGVALIVPAVVISRRRGFGAAGRHETRPPFWRS
                     LREAAWGLAAPVLILGGMRVGWFTPTEAAVVAVAYGLFIGIAIHRSIGWRDLYPVFRE
                     ATEVSAVIMIVLALAGIFAYAVNTLGIADPLVAFVQRLRLGSHGTLILLFVVMSVLGM
                     VLDGVSIFLVFLPLLYPLMVAFHWDPVWFGVLLTMKIAIGQFTPPLAINLMVSCRLAR
                     VRIDETIPWVAWMIVSFVIAALVVLFVPETALWLPRRLGY"
     gene            3366..4403
                     /gene="dctP"
                     /locus_tag="VFF72_06530"
     CDS             3366..4403
                     /gene="dctP"
                     /locus_tag="VFF72_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZV92856.1"
                     /translation="MTLDRRTLVKTLAATGVAAVAAPRFVLAQPSYRAEYKMSTVVPP
                     AFAWGKGGEIFATLVRERTAGRINIKQYPGASLVQGQQDREFSAMRQGVIDVLCGAPV
                     NWTSTVPQLGVFTLPFIMPDHRAWDAVMASDAVVKPYFEMVRKAGAEPLAIGETGYRQ
                     ISNSKHPIVHPDDMKGLKIRVVGSPMYGEIMSSMGANPTFMSWADAQPALASGAIDGQ
                     ENPLEVFLAAKINTLGQKHVTKWNYSNDILLFAIAAPVWASWTPADQKIVRDAAVDAA
                     RQQVALVRKLFAEDVQRVSALGVAVHVPDGAEMAAWRIATRRPYARYKAQTQPQLVTT
                     IEEVVATTRKG"
     gene            4400..4912
                     /locus_tag="VFF72_06535"
     CDS             4400..4912
                     /locus_tag="VFF72_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZV92857.1"
                     /translation="MSRARAPEGGTRPAPAALPLDSSVFFKLVRVVNLTARPFSESIG
                     RMHRLTLNAWRVLLVLANHPGVAASEVVALTGLDKMSVSRALTSLVRQGRVVRKGDAA
                     DRRRMLLRLSAEGERLYERIGTPASERERSLFRGVGAAEQATLGRMLDRLIDNLVAAD
                     EAKTLQRSAD"
BASE COUNT          718 a         1756 c         1689 g          853 t
ORIGIN      
        1 ctggctcgaa gctcgcccgg atgcgacaaa gacgcacggc aatccgccga tctctgttgc
       61 aggtatttcc atgactccca aaacctcgat gaccaccgac ctcatttcct ccgaacgtgt
      121 ggaaggcact tccgtataca acagcgccgg cgacaagctc ggctcgatcg acgatctcgt
      181 gatcgacaag cgcagcggcc aggttcgcta cgcggtgatg gagttcggcg gcttcctcgg
      241 catcgacacc gaccgctatc cgattccgtg gaacatgctg cgctacgaca ccgacaagga
      301 cggctatgtc gtcccgctcg acaaggccaa gctcgaaaat gcgccgaagt accgcgacga
      361 ggacgtgccg gtgtacgacc tcacgtacgg caagcgcgtc aacggctact acggcgtcga
      421 ctggtagatc tccaacgtcg acttcgcgac tgcgccgttg tcgagacggc tgcgtcgcac
      481 ggcaatgtcg cgccagaggc ctttttgaac ggcctctggc acttcttgct gctgaataaa
      541 atgggttgcg cctgcgtggt gcctgcaggc ttcatcctcg gcctgtgatg agcaccggtg
      601 cgaatgccgc gagaactcac gattgccact cttccaggaa tgccgcagac cgacgagccc
      661 gaacccgacg cgttgccggt cgagccagac gacggcacga cgcttccggg gcgcctgccc
      721 gaagagggcc ccggtcacga gcctgccgat ccgcccaagg cgtgacgtcc tccgtccgac
      781 cggcggcagc ctcgggcccg aggccgagcg cacaatcgcg gcgcgccgtg cggcgcgtgg
      841 agtctcgtgt cgatgcggcg tgtctgggct ggcgtgatcc ttgttcttgc cgtcctgggc
      901 tacggtgcct gggcgtccga cttcatcacg ctgcaaggcg agcgaaccgt ctacacggtc
      961 ggctgccagg gcggcgaatg gcaaggcgag gtctgctcgg gaaagctggt cgccggcgac
     1021 cgctaccgtt accgggcgct caagccgcac catgaagtga tcttctggaa ggtcggcgcc
     1081 cgcgaccggt cgggcaagtt cgatcgctgc gacatccatg acggccgcaa ctgggtttgc
     1141 gcgccgagcg acgatgcgtc gcgctcgatc acgctggcca tggacggcgg cgtcccggtg
     1201 cccgggccgc ccaccgtgac gcgaccgttt cgagccgtct cgaaatggcg ctggatcctg
     1261 ctcaggcgag gcctgtcgcg caagaaggtc gtgccgctga tcgcggcggc gccgtctccg
     1321 gcccattgat cgaggcgcag ccgcggatcg cgacatcgcg gctgcgggaa aacgcgagac
     1381 gcggctgacg gtaacgatcg ttaccatcct cgcggcccgg cacggccggc gcgagggagc
     1441 gcggtgaaag atccgaagat cgaggagcgc ctgggcgtcg cctgcatggt ggtgctgacc
     1501 gtgctcacgc tcggcaacgt cgtcacccgc tatctcaccg acgaatcgtt cgcctggacc
     1561 gaggagatct cggtcttcct cctggtcgtc atgacgctgg ccggggctgc atcgattgcc
     1621 ggccgcgacg cccacatccg gatcgagtac ttctacgacg gcggcagcga cacgcgaagg
     1681 cggcggctgc gccttctcgc ggccgcgtcg accgcgcttc tcttcgccgg gctcgcggtg
     1741 ctgttcggca tcgacctcgc cgacgagatc cgctggaacg agacatcgat ggggctgggc
     1801 gtgccgcggt ggtggttcac ggcggccatc ccgccgctgt gcatcgccat cagcgcgcgc
     1861 gccgtgctgg ccgggcgccg cgccctgcgg ggcctgccgg cgtcgcctgc ggcgccgttc
     1921 gatcccgaag gccggctctg aaatctgcgc agcacggatg atcaccgcca cgctcgtcgt
     1981 cctcttcgtc gggctgatgc tggcgggcgt gccgatcggc gtcgcgctcg gcatcggcgg
     2041 catcgtcgcg atcgccgcat cgaaccccgg ttcaccggcc tggggcctgc tcgcagcacc
     2101 gcagaacatg cacgcgtcgg tcgccaagta cccgttgctg gcgctgccga tgttcgtcct
     2161 cgtcggctcg atcttcgacc gctccggcgt cgcccggcgc ctcgtcacct tcgcacaggc
     2221 gtgcgtcgga cacgcgccgg gcatgctgcc gatggtggcc atcctcgtgg ccatgctgct
     2281 cggcggcatc tccggttccg gtccggcgac ggcggcggcg gtcggcggcg tgatgatcgc
     2341 ggcgatgaat cgtgccggct atccaccggc gttctcggcc agcgtcgtcg gcgccgcggc
     2401 ggccaccgac atcctcattc cgccttcgat cgcgttcatc gtctacagcg tcaacgtccc
     2461 cggcgcctcg gtgccggcgc tgttcgccgc cggcatggtg ccggggctgc tcgccggcgt
     2521 cgcgctgatc gtgccggccg tcgtcatctc gcgccgccgc ggcttcggcg cggcggggcg
     2581 gcacgagacg cggccgcctt tctggcgatc cctgcgcgag gcggcgtggg gtctcgcggc
     2641 gccggtcctc atcctcggcg gcatgcgagt gggctggttc acgccgaccg aggcggcggt
     2701 ggtcgccgtg gcctacggcc tgttcatcgg catcgccatc caccgctcga tcggctggcg
     2761 cgacctctat cccgtctttc gcgaggcgac cgaagtctcg gccgtgatca tgatcgtcct
     2821 cgcgctcgcc ggcatcttcg cgtacgcggt caacacgctc ggcatcgccg acccgctcgt
     2881 cgccttcgtg cagcgcctgc gcctgggctc gcacggcacg ctcatcctgc tcttcgtcgt
     2941 gatgagcgtt ctcgggatgg tgctcgacgg cgtctcgatc ttcctcgtct tcctgccgct
     3001 gctgtacccg ctgatggtcg cgtttcactg ggacccggtc tggttcggcg tcttgctgac
     3061 gatgaagatc gccatcggcc agttcacgcc gccgttggcg atcaacctga tggtgtcgtg
     3121 ccggctcgcc cgcgtccgca tcgacgagac gattccctgg gtcgcctgga tgatcgtctc
     3181 gttcgtcatc gccgccctcg tcgtcctttt cgttcccgag acggcgctct ggctgccgcg
     3241 tcggctcggc tactgacggg catggctcgg gcgtcgtccg cgctgccttg ccgtccttgc
     3301 gacggcaatg cccgttttcg tcgttccgca ggacccttcc tcgccttctt ccaggagaca
     3361 tcgccatgac cctcgatcgc cgcaccctcg tcaagaccct ggccgcgacc ggcgtcgccg
     3421 ccgtcgcagc tccccgcttc gtgctcgcgc agccgagcta ccgcgccgaa tacaagatgt
     3481 cgacggtcgt gccgccggcc ttcgcctggg gcaagggcgg cgagatcttc gcgacgctgg
     3541 tgcgcgagcg gaccgcgggt cgcatcaaca tcaagcagta cccgggcgcg tcgctggtgc
     3601 agggccagca ggaccgcgag ttctcggcga tgcgccaggg ggtgatcgac gtgctttgcg
     3661 gcgccccggt gaactggacg tcgacggtgc cgcagctggg cgtcttcacg ctgccgttca
     3721 tcatgcccga tcaccgggca tgggacgccg tgatggcgag cgacgccgtc gtcaagccct
     3781 acttcgagat ggtccgcaag gccggcgccg aaccgctggc gatcggcgag acgggctatc
     3841 gacagatcag caacagcaag caccccatcg tccatcccga cgacatgaag gggttgaaga
     3901 tccgcgtcgt cggcagcccg atgtacggcg agatcatgag ctcgatgggc gccaacccga
     3961 ccttcatgag ctgggccgac gcccagccgg cgctcgcgtc gggggcgatc gacggccagg
     4021 aaaatccgct cgaggtcttt ctcgccgcga agatcaacac gctcggccag aaacacgtga
     4081 cgaagtggaa ctactcgaac gacatcctcc tcttcgcgat cgccgcgccg gtctgggcca
     4141 gctggacgcc cgccgaccag aagatcgtcc gcgacgccgc cgtcgacgcg gcgcgacagc
     4201 aggtggcgct ggttcgcaag ctctttgccg aggacgtgca gcgcgtctcg gcgctcggcg
     4261 tcgcggtgca cgtgccggac ggcgccgaga tggcggcctg gcggatcgcc acgcgtcgcc
     4321 cgtatgcgcg ctacaaggcg cagacgcagc cgcagctcgt gacgacgatc gaggaggtcg
     4381 tcgcgacgac gcgcaaggga tgagccgcgc ccgggcgcct gagggcggca cgcggccggc
     4441 gcctgccgcg ctgcctctcg acagctccgt cttcttcaag ctggtgcgcg tcgtcaacct
     4501 gacggcgcga cccttctccg aatcgatcgg ccggatgcac cggctcacgc tcaatgcctg
     4561 gcgcgtgttg ctcgtgctcg cgaaccatcc gggcgtcgcg gcaagcgagg tcgtggcact
     4621 gacggggctc gacaagatga gcgtcagccg tgccctgacg tcgctggtgc gccagggccg
     4681 ggtcgtccgc aagggcgacg ccgccgaccg gcgacggatg ctgctgcgcc tgagcgccga
     4741 gggcgagcgc ctctacgagc ggatcggcac gccggcgagc gagcgcgaac gaagcctctt
     4801 tcgcggcgtc ggcgccgccg agcaggcgac gctcggccgg atgctcgacc gcctgatcga
     4861 caacctcgtc gccgccgacg aggcgaagac gttgcaacgg tccgccgatt gagatcgcag
     4921 cgttcgccat ctgcaggacg agggccagtt cggcaacgcg ccgatcttct cggcgttcaa
     4981 ctacgcaagg ggcgcgttgg gacggcccgg cgggcc
//
LOCUS       DASRWS010000346         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2427097, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000346 DASRWS010000000
VERSION     DASRWS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427097"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /gene="rpoN"
                     /locus_tag="VFF72_06540"
     CDS             <1..1438
                     /gene="rpoN"
                     /locus_tag="VFF72_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZV92858.1"
                     /translation="HLELATFIQQELEKNPLLQETAADDGPAPERDTPPIDTPRDTAE
                     ALAAAAPALADADDRWTREFADRGGDAKAAPREDAPEALDFVAERPMSLAAHVLEQIE
                     LIFADAADRRIALKLAEGLDEAGYCRLEAPDVAEAMATDLATVERVWARLRSVEPAGL
                     FARTLAECLGSQLAERDRLDPAMKALLDNLDLVAAGELSQLRRRCGVDDEDLRDMLAE
                     LRTLDPRPGQKFDFEPLQPVAPDLFLTPAKDPETGEEGWHIELNTDALPKVLVDRNYH
                     ATLMKGARAKSERDFVAERFQSANWLVKTLEQRATTILKVAREIVRQQDGFFRHGVSA
                     LKPLVLRDIALATELHESTVSRVTSNKYIATPRGIFELKYFFTSSLPARTPGTVVSSE
                     AVRSRIRRLIGAENRRQPLSDDRIVELLKGEGIEIARRTVAKYREAMRIPSSAERRRL
                     GRLGIGRGVAAAVPPAAVAAQVVTGSAD"
     gene            1552..2022
                     /locus_tag="VFF72_06545"
     CDS             1552..2022
                     /locus_tag="VFF72_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZV92859.1"
                     /translation="MEIADLLPGPDAVLACVKASGKKALLADLASRAATLFKVDDRRL
                     FDRLVERERLGSTGIGGGIAIPHGRMSALAAPVGLFARLAQPIDFDSIDERPVDLVFL
                     LMAPEGAGADHLKALARVSRLLRDRSLVEKLRATDNGDALYALLVESIQAQSAA"
     gene            complement(2042..2320)
                     /locus_tag="VFF72_06550"
     CDS             complement(2042..2320)
                     /locus_tag="VFF72_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92860.1"
                     /translation="MNSPRADAAKEVAMENNQTSNKTTTAFTPLADDAFLSLGAQQIA
                     YIKPVNLPDGAHAIGIFSADGKPMAAAPTVEVAEALLRQHDLEPALVH"
BASE COUNT          355 a          790 c          818 g          375 t
ORIGIN      
        1 gcatctcgag ctggcgacct tcatccagca ggagctggaa aagaacccct tgctgcagga
       61 gaccgcggcc gacgacggtc cggccccgga aagggacacc ccgccgatcg acaccccgcg
      121 cgataccgct gaagcgctgg cggctgccgc gccggcgctc gcggatgccg atgaccgctg
      181 gacccgggaa ttcgccgacc gtggcggcga tgccaaggcg gcgccgcgcg aggatgcacc
      241 cgaggcgctg gacttcgtgg ccgagcggcc tatgtcgctg gcggcccatg tgctggagca
      301 gatcgaactg atcttcgccg acgctgccga tcggcgaatt gcgctgaagc tcgccgaagg
      361 actcgacgag gcgggctatt gccgtctcga ggcccccgac gtcgccgagg cgatggccac
      421 cgatctcgcg accgtcgagc gtgtgtgggc gcgactgcgc tctgtcgagc cggcgggcct
      481 gttcgcccgc acgctggcgg agtgcctggg ctcccagctc gccgagcgcg accgcctcga
      541 tccggcgatg aaggcgctgc tcgacaatct cgatctcgtc gccgccggcg agctctctca
      601 gctgcgccgg cgctgtggtg tcgacgacga ggatttgcgc gacatgctgg cggagctgcg
      661 cacgctcgat ccgcggcccg gccagaagtt cgacttcgag ccgctccagc cggtcgcgcc
      721 cgatctcttc ctgacgccgg cgaaggatcc ggagaccggt gaggaaggct ggcacatcga
      781 gctcaacacc gatgcgctgc ccaaggtgct ggtcgatcgc aactaccacg cgacgctgat
      841 gaagggcgct cgggccaagt ccgagcgcga cttcgtcgcc gagcgcttcc agtcggccaa
      901 ctggctggtg aagacgctcg agcagcgcgc caccacgatc ctcaaggtcg cgcgcgagat
      961 cgtgcgccag caggacggct tcttccgcca cggcgtcagc gccttgaagc cgctggtgct
     1021 gcgcgacatt gcgctggcaa ccgagctgca cgagagcacg gtgagtcgcg tcacctcgaa
     1081 caagtacatc gccacgccgc gcggcatctt cgagctcaag tatttcttca cctcgtcgct
     1141 gccggcgcgc acgcctggca cggtggtctc gtcggaggcg gtgcgctcgc ggatccgtcg
     1201 cctgatcggg gccgagaacc ggcgccagcc gctgtcggat gaccgcatcg tcgagctgct
     1261 gaagggcgag ggcatcgaga tcgcgcgacg cacggtcgcg aagtatcggg aggcgatgcg
     1321 catcccctcg tcagccgagc gccggcgcct cggccgtctc ggaatcgggc gcggcgtcgc
     1381 tgcggccgtg ccgcccgccg cggtggcggc gcaggtcgtg acgggctccg ccgattgagc
     1441 cctcgaacgg tcgcgactac cgggcttgac cgtcccggaa gaggcgctta taggggcgca
     1501 cctcacgccg gacgaacagg gcgtccgagt cgctaacttg ttggtgatat tgtggaaatt
     1561 gccgatttgc tacccggccc ggacgctgtc ctggcctgcg tgaaggcgtc cggcaagaag
     1621 gcgctgctgg ccgatctggc aagtcgggcg gccaccttgt tcaaggtcga cgaccggcgg
     1681 ctgttcgacc gtctggtcga gcgcgagcga ctcggatcga ccggcatcgg tggcggcatt
     1741 gccatcccgc atgggcgcat gagcgccctg gccgcgccgg tcggcctgtt cgcccggctc
     1801 gcccagccga tcgacttcga ttcgatcgac gagcggccgg tggacctcgt gttcctgctg
     1861 atggcgcccg aaggcgccgg cgccgatcac ctcaaggccc tggcccgcgt gtcgcgcctg
     1921 ctgcgcgacc gctcgctggt ggagaaattg cgcgcgaccg acaacggcga cgcgctctac
     1981 gctctcctcg tcgaatcgat ccaggcgcaa agcgcggcat gaagcgccgc gcggcggcgg
     2041 atcaatggac cagcgccggc tcgagatcgt gctggcgcag caacgcttcg gcgacctcga
     2101 cggtcggcgc ggccgccatc ggcttgccat cggccgagaa gatgccgatc gcatgcgcac
     2161 cgtccgggag attcaccggc ttgatgtagg cgatctgctg agcacccagg ctgaggaagg
     2221 catcgtccgc cagcggggtg aaggcggtgg tcgtcttgtt gctggtctgg ttgttttcca
     2281 tggctacctc cttggcagca tcagcgcggg ggctgttcat ttgagatggg cacggcgt
//
LOCUS       DASRWS010000347         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2720720, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000347 DASRWS010000000
VERSION     DASRWS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720720"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="VFF72_06555"
     CDS             complement(<1..1699)
                     /locus_tag="VFF72_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZV92861.1"
                     /translation="MIRYVIDASATLSHDFAVTLTVPRPRGRVRIALPVWVPGSYLVR
                     EFSRHLSDLRAGQGANALELRQLDKTSWSVDCPDDEDLVLRYRIHAFDVSVRAAFLDA
                     TRGFFNGTSVCLRVDGRAGEPHGLAITGLAPGWEVATTLTPAGGPSRHEFVATGYDEL
                     VDHPVELGRFWRGTFEAAGTPHELVVAGALPDFDPARLQADIARVCETEIAFWHGTGR
                     PPFDRYLFLLNAVDEGRGGLEHRASTALVCPRRDLPRLPGPTEGDTREASDGYVDVLG
                     LFAHEYFHAWNVKRLRPRDFATLDYGRENYSELLWFFEGFTSYYDDLLVARAGLIDAP
                     RYLKLLSKTMSAVAATPGRERQSVAAASFDAWVKYYRPDENTPNATVSYYAKGSLVAL
                     ALDLTLRSESRGSLDDVMRRLWSGSGGGLIDEAGIAAAIEAVGGRSYAAEIAAWVHGT
                     GELPVEELLSRVGVGLERQPATLAQRLGVRVSEGPFTGIKVSHVLRGQSAERAGLAPG
                     DELLAADGWRLRRLDDALRVLGNPIRLLVSRDQRVFEVAVDPSTLAGAEAGAVQLRVA
                     "
     gene            1879..2643
                     /locus_tag="VFF72_06560"
     CDS             1879..2643
                     /locus_tag="VFF72_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZV92862.1"
                     /translation="MNSDAYVRPARIGPEEWAARIQLAAAYRIFDHLGWTELIYNHIS
                     LRVPGEADHYLINPFGLHYSEVCASNLVKVDLAGQVVGHSDWPINPAGITFHGAIHAT
                     LDDAHCVMHLHTTATQAVCCLKEGLSFSNFYAAQLYGKVAYHDFEGITVHADEGARIL
                     KSAQGRPVLLLRNHGPVTIGQTLPMALALMWTVNRACEIQWATHSMGETLPIPRGVLE
                     KCVADSLQFNPKYGAGEDVFAALQRLVDRKDPGYRM"
     gene            complement(2728..3465)
                     /locus_tag="VFF72_06565"
     CDS             complement(2728..3465)
                     /locus_tag="VFF72_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZV92863.1"
                     /translation="MKFFTSRTFAAFALAACLVPAAFADEAAIRKNITERLPDFPKID
                     EVTKSVIPGIWEIRIGHEVLYTDEQASYIIEGRIIDTKSRVNLTEERVAKLTAIDFKS
                     LQLKDAIVWKQGNGDRKLVVFADPNCGFCKHFERDTLQTVKDVTVYTFLYPILGGDSP
                     AKAKAIWCAKDKTSVWRDWMVKGTPIAPSGDCDTSALARNYAFARAHGINGTPGLVFE
                     DGRKVSGAINNDALEKNFVSARARSPG"
     gene            complement(3471..3869)
                     /locus_tag="VFF72_06570"
     CDS             complement(3471..3869)
                     /locus_tag="VFF72_06570"
                     /inference="COORDINATES: protein motif:HMM:NF020080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZV92864.1"
                     /translation="MSLVSSRAAWPLLRGRATTWCGPGWVLLGDAAHVVHPLAGQGLN
                     LGLADVAALVDVIEAREPWRNLGDERLLRRYVRQRAGPTWAMLRITDGLQGLFAAEPA
                     PVRDLRNNGLNLVNRLTPLKRWLTARALDS"
BASE COUNT          643 a         1333 c         1320 g          615 t
ORIGIN      
        1 cggcgacacg caactgcacc gcaccggcct cggcgccggc cagcgtcgac ggatcgaccg
       61 ccacttcgaa gacgcgctgg tcacgggaga cgagcagtcg gatcgggttg ccgaggactc
      121 gcaaggcatc gtcgagacgg cgcaggcgcc agccgtcggc ggcgagcaat tcgtcgcccg
      181 gcgccaggcc agcgcgctct gccgactggc cacgcaggac gtgcgacacc ttgattccgg
      241 tgaacggccc ctcgctgaca cgaacgccca gccgttgcgc gagcgtcgcc ggctgccgtt
      301 cgaggccaac gccgacgcgg ctcagcagct cctcgaccgg cagctcgccg gtgccgtgca
      361 cccaggccgc gatttccgcg gcataggaac gaccgccgac ggcctcgatc gccgcggcga
      421 tcccggcttc gtcgatgagg ccgccgccgc tgccggacca gaggcggcgc atcacgtcgt
      481 cgagcgaacc acgactttcg ctgcgcaagg tcagatcgag cgcgagggcg acgagcgatc
      541 ctttcgcgta gtagctgacg gtcgcgttgg gcgtgttctc gtcgggccgg tagtacttga
      601 cccaggcgtc gaagctggcc gcggcgacgc tttgccgttc gcggcccggc gtcgctgcga
      661 cggcgctcat cgtcttggag agcagcttca ggtagcgagg cgcgtcgatc aggcccgccc
      721 gggcgaccag caggtcgtcg tagtacgacg tgaacccttc gaagaaccag agcagctcgc
      781 tgtagttctc gcgaccgtag tcgagcgtcg cgaagtcgcg cggcctgagc ctcttgacgt
      841 tccaggcgtg gaaatattca tgggcgaaaa ggccgagcac gtcgacgtag ccatcgctgg
      901 cctccctggt gtcgccctcg gtcggccccg gcagccgggg caggtcgcgc cgcggacaga
      961 cgagcgccgt gctcgcacgg tgctcgagac cgccgcggcc ctcgtcgacg gcattgagaa
     1021 gaaaaaggta gcgatcgaac ggagggcggc ccgtgccgtg ccagaaagcg atctcggtct
     1081 cgcagacgcg ggcgatgtcg gcctgcagcc gcgccgggtc gaaatcgggg agtgcccctg
     1141 ccacgaccag ctcgtgcggc gttcccgcgg cttcgaacgt gccgcgccag aagcgtccga
     1201 gctcgaccgg gtgatcgacg agctcgtcgt agccggtggc gacgaattcg tgccgcgacg
     1261 gaccgcctgc cggcgtcagg gtcgtcgcca cctcccagcc gggcgcgaga cccgtgatcg
     1321 cgaggccgtg cggctcgccc gcccgtccgt ccacgcgcag gcagacgctg gtgccgttga
     1381 agaagccgcg ggtcgcgtcg agaaaggccg cacgcaccga cacgtcgaag gcgtggatgc
     1441 gatagcgaag cacgagatcc tcgtcgtccg ggcagtcgac cgaccagctt gtcttgtcga
     1501 gctggcggag ttcgagcgcg ttcgcgccct gcccggcgcg aaggtccgaa aggtggcgcg
     1561 agaactcgcg caccagatag ctgccgggca cccacaccgg cagcgcaatg cgcacccggc
     1621 cccgcggccg cggcacggtc agggtgacgg cgaagtcgtg gctgagcgtc gccgaagcgt
     1681 cgatgacgta gcggatcatg ggcggggatt gtcgcctccg cctgcgccaa cgcatggcca
     1741 tggtggctcc gacatcgaag cgctgacggc gctcggggtc gtgtcgcggc ccgccattcg
     1801 acgtgcccgt ctggacgatg acgcctcgat ttccagtacg cttcgaattc cctcgacctg
     1861 ccagcgcgcc ctccggccat gaattccgac gcctacgtcc gacccgctcg aatcggcccc
     1921 gaggaatggg cggcccggat ccagctcgcc gccgcctatc gcatcttcga tcacctcggc
     1981 tggaccgagc tgatctacaa ccacatctcg ctgcgtgttc ccggcgaggc cgatcactac
     2041 ctcatcaacc ccttcggcct gcactattcc gaggtctgcg cctccaatct ggtgaaggtc
     2101 gacctcgccg gccaggtcgt cggccattcc gactggccga tcaatccggc gggaatcacg
     2161 ttccacggcg cgatccacgc gacgcttgac gatgcgcatt gcgtgatgca tctgcacacc
     2221 acggcgacgc aggccgtctg ctgcctgaag gagggcttgt cgttttcgaa cttctatgcg
     2281 gcgcagctct acggcaaggt ggcgtaccac gacttcgagg gcatcaccgt ccacgccgac
     2341 gaaggggccc gcatcctgaa gagcgcccaa gggcgcccgg tgctcctgct gcgcaaccac
     2401 ggcccggtga cgatcggcca gacgctgccg atggcgctgg ctctgatgtg gaccgtcaat
     2461 cgcgcctgcg aaatccagtg ggccacgcac tcgatgggcg agacgctgcc gatccctcgg
     2521 ggcgtgctcg agaagtgcgt cgccgactcg ctgcagttca acccgaagta cggcgcgggc
     2581 gaggacgtgt tcgccgcgct gcagcgtctc gtcgaccgca aggatccggg ctaccggatg
     2641 taggaaacgg gcggcggcgc gatgctgccg gatgctgctg aaggccggat cagccggacg
     2701 atccgccagc ccgccgctaa ctcccgatca gcccggcgaa cgggcgcggg cgctgacgaa
     2761 attcttctcg agcgcgtcgt tgttgatcgc gcccgagacc ttgcgcccgt cttcgaagac
     2821 gagacccggc gtgccgttga tgccgtgggc acgggcaaag gcgtagttgc gcgccagcgc
     2881 cgacgtgtcg caatcgccgc tcggtgcgat cggcgtgccc ttgaccatcc agtcgcgcca
     2941 gaccgaggtc ttgtccttgg cgcaccagat cgccttggcc ttggccggcg agtcgccacc
     3001 gaggatggga tagagaaagg tatagacggt gacgtccttg accgtctgca gcgtgtcgcg
     3061 ctcgaagtgc ttgcaaaagc cgcaattcgg atcggcgaag acgacgagct tgcgatcgcc
     3121 gttgccctgc ttccagacga tcgcgtcctt caactgcagg ctcttgaagt cgatcgccgt
     3181 cagcttggcg acccgctcct cggtgaggtt gacacgcgac ttcgtgtcga tgatgcggcc
     3241 ttcgatgatg tagctcgcct gctcgtcggt gtagagcact tcatggccga tgcgaatctc
     3301 ccagatgccc gggatgaccg acttcgtgac ttcgtcgatc ttcggaaaat cgggcagccg
     3361 ctcggtgatg ttcttgcgga tcgccgcctc gtcggcgaac gccgccggaa caaggcatgc
     3421 cgcgagcgcg aaggcggcga aggtgcgcga ggtgaagaac ttcatgtgcg tcaggagtcg
     3481 agggcccgcg cggtcagcca gcgcttgagc ggcgtgaggc ggttgacgag attgagccca
     3541 ttgttgcgga gatcgcggac cggcgcgggt tcggcagcga acaatccctg caatccgtcg
     3601 gtaatccgca gcatggccca ggtcgggccg gcgcgctgcc gcacgtaccg ccgaagcaac
     3661 cgctcgtcgc caagattgcg ccacggctca cgcgcctcga tgacatcgac gagcgcggcg
     3721 acgtcggcaa gaccgaggtt gagcccctgt ccggcaagcg gatgcacgac atgcgccgcg
     3781 tcgccgagca gcacccagcc cgggccgcac cacgtcgtcg cccgccctcg caacagcggc
     3841 caggcggctc gtgacgaaac cagcgacatt ccgccgacgt cgccgccggt ggcttgcgcc
     3901 agctcgtgtt c
//
LOCUS       DASRWS010000348         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2720823, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000348 DASRWS010000000
VERSION     DASRWS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720823"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFF72_06575"
     CDS             <1..817
                     /locus_tag="VFF72_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZV92865.1"
                     /translation="GDTIQVMAPEGRFFVPLDPGASRHYLGIAGGSGITPILSIMKTV
                     LAAEPRSRFTLIYGNRKLRSTMFMEELEDLKNRYLSRLTLHLLFSQEATELPIQGGRL
                     DRAKIGEFLSGGALVDPRRIDHVFVCGPHGVNDEAEAALLAAGVAAERIHIERFGVPD
                     PAGTARLASHQPLEGDAADARVTVIRDGITSEIDFHSAQGNLLEAAAAAGLEVPFSCK
                     SGVCCTCRARLLEGEVRMARNFALEKHEVAAGFILTCQSHPLTERVVISFDER"
     gene            902..1561
                     /locus_tag="VFF72_06580"
     CDS             902..1561
                     /locus_tag="VFF72_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV92866.1"
                     /translation="MPRGRAHGYDEQREQILGRAARLFARRGYTATTMNEVAEACGVS
                     KPSLYHYVRDKHQLLVEIAESHVTRLAAIVDRVVAETTRPEERVRCLIAAFLAIYAEA
                     QAEHRVLTEDVRFLAPADRRRILDGERKVVAAFADAIAQARPELGREALDKPLAMLLF
                     GMMNWMFTWLKPRGALTHAEMAPIVADLFFGGLAAVRAEGGAPPAPPRTQRRPTPEFQ
                     P"
     gene            1558..>2603
                     /locus_tag="VFF72_06585"
     CDS             1558..>2603
                     /locus_tag="VFF72_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZV92867.1"
                     /translation="MSGTPVLQSLIADRWIGTTASAALASAIDGRTVGHTHAESIDFE
                     EALAHARRTGLPALLAMDFQQRGARLKALAAYLNERKEALYEISRHTGATRSDSWVDI
                     EGGTGTLYAYASMGGNELPSGNVVHEGPAVPLGKKGQFAGTHILVPRGGVAVHINAFN
                     FPVWGLLEKFAPTFLAAMPCIAKPATATSYLTEAAVRLMLESGLLPPGSLQLVIGSTG
                     DLLDRLDSQDVVTFTGSADTALKLRGNRNLLARSIPFNAEADSLNAAILGPDVSPDDE
                     EFDLFVKEVVREMTVKAGQKCTAIRRAIVPRRHLDALATKLRERLAKVSVGDPAIEGV
                     KMGALASMAQHRDV"
BASE COUNT          399 a          931 c          886 g          387 t
ORIGIN      
        1 cggcgacacg atccaggtga tggcgcccga agggcggttc ttcgtgccgc tcgatcccgg
       61 ggcgtcgcgc cactacctgg gcatcgccgg cggcagcggc atcacgccga tcctgtcgat
      121 catgaagacg gtgctggcgg ccgagccgcg ctcgcgcttc acgctgatct acggcaatcg
      181 aaagctgcgt tcgacgatgt tcatggaaga gctcgaggac ctgaagaacc ggtatctctc
      241 gcgcctgacg ctgcacctgc tgttctcgca ggaggcgacc gagctgccga tccagggcgg
      301 ccggctcgat cgcgcgaaga tcggcgagtt tctctccggc ggcgccctcg tcgacccgcg
      361 acggatcgat cacgtcttcg tctgcggacc gcacggcgtc aacgacgaag cggaggcggc
      421 actgctcgcg gcgggcgttg ccgccgagcg gatccacatc gagcgcttcg gcgttcccga
      481 tccggcgggc acggcgcgcc tggcgtcgca ccagccgctc gaaggcgacg cggcggacgc
      541 ccgggtgacc gtcatccgcg acggcatcac cagcgagatc gacttccact cggcgcaggg
      601 caatctcctc gaggccgcgg ccgcggcggg cctggaagtg ccgttctcgt gcaagtcggg
      661 cgtttgctgc acgtgccggg cccgtcttct cgaaggcgag gtgcggatgg cgcgcaactt
      721 cgcgctcgag aagcacgagg tggccgccgg cttcatcctg acctgccagt cgcatccgtt
      781 gaccgagcgg gtcgtgatct cgttcgacga gcgatgagcg tcatgccgcg cccggcgccg
      841 cgcagccgcc gccggagcgc gcggcgttcg tcgccggcga ggcgacggga cgagcgctga
      901 catgccccgc ggacgtgccc acggctacga cgagcagcgc gagcagatcc tcggtcgcgc
      961 ggcacgcctg ttcgcgcggc gcggctacac ggcgacgacg atgaacgagg tcgccgaggc
     1021 gtgcggcgtc tcgaagccgt cgctctatca ctacgtccgc gacaagcacc agttgctcgt
     1081 cgagatcgct gaatcccacg tcacgcggct cgctgccatc gtcgaccgcg tcgtcgccga
     1141 gacgacccgt cccgaggagc gtgtgcgctg cctgatcgcc gccttccttg ccatctacgc
     1201 cgaagcgcag gccgagcatc gcgtgctgac cgaggacgtc aggttcctcg cgccggccga
     1261 tcggcgccgc atcctcgatg gcgaacgcaa ggtcgtggcg gccttcgccg acgcgatcgc
     1321 ccaggcgcgt cccgagctgg gtcgagaagc gctcgacaag ccgctggcga tgctgctctt
     1381 cggcatgatg aactggatgt tcacgtggct gaagccgcgc ggtgcgctga cccatgccga
     1441 gatggcgccc atcgtcgccg atctgttctt cggcggcctg gccgccgtgc gtgccgaagg
     1501 cggcgcgcca cccgcaccgc cgcgaacgca gcgtcgcccc accccggagt tccagccatg
     1561 agcggcaccc ccgtcctgca gagcctgatc gccgatcgat ggatcggcac gaccgcttcg
     1621 gcggccctgg cgagcgcgat cgacggccgc accgtcggac atacccacgc cgaatcgatc
     1681 gacttcgaag aggcgctcgc ccatgcccgg cggaccggac tgccggcctt gctggcgatg
     1741 gacttccagc agcgcggggc gcggctcaag gcgctcgccg cctacctcaa cgaacgcaag
     1801 gaagcgctct acgagatctc gcgccatacc ggcgcgacgc gcagcgacag ctgggtcgac
     1861 atcgagggcg gcaccggcac gctctacgcc tacgcgtcga tgggcggcaa cgagctgccc
     1921 tcgggcaacg tcgtccacga ggggccggcc gtgccgctcg gcaagaaggg ccagtttgcc
     1981 ggcacgcaca tcctcgtgcc gcggggcggc gtcgccgtgc acatcaatgc cttcaatttc
     2041 ccggtctggg gcctgctcga gaaattcgcg ccgaccttcc tcgccgccat gccctgcatc
     2101 gccaagcccg cgacggcgac gagctatctc accgaggccg ccgtgcgcct gatgttggag
     2161 tcgggcctgc tgccgccggg gagcctgcag ctcgtgatcg gctcgaccgg cgacctgctc
     2221 gaccggctcg acagccagga cgtcgtcacc ttcaccggct cggccgacac ggcgctcaag
     2281 ctgcgcggca atcgcaacct gctcgccagg tcgattccct tcaacgccga agccgattcg
     2341 ctcaatgccg cgatcctcgg ccccgacgtc tcgcccgacg acgaggagtt cgatctcttc
     2401 gtcaaggaag tcgtgcgcga gatgaccgtg aaggcggggc agaagtgcac ggcgatccgc
     2461 cgcgccatcg tgccgcgcag gcatctcgac gcgctcgcga cgaagctgcg cgagcggctc
     2521 gccaaggtga gtgtgggcga cccggcgatc gagggcgtga agatgggcgc gctcgcgtcg
     2581 atggcgcagc atcgcgacgt cag
//
LOCUS       DASRWS010000349         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_143609, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000349 DASRWS010000000
VERSION     DASRWS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143609"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /gene="pgk"
                     /locus_tag="VFF72_06590"
     CDS             complement(<1..639)
                     /gene="pgk"
                     /locus_tag="VFF72_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZV92868.1"
                     /translation="MTLPRFTDLVDSGRLAGKRVFIRADLNVPQDEDGRITEDTRIRA
                     SLPCIEQALGAGAAVMVTSHLGRPTEGDAKPRDTLAPVAERLGQLLGRPAPLRRDWLD
                     GVEVAPGSLVVLENCRLHKGEKKNDAALARKMAALCDVYVNDAFGTAHRAEATTYGIA
                     EFAPVAVAGPLLAAEVDAITTALADPRRPLVAIVAGSKVSTKLAILQSLAKKV"
     gene            829..>3711
                     /locus_tag="VFF72_06595"
     CDS             829..>3711
                     /locus_tag="VFF72_06595"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZV92869.1"
                     /translation="MLARLFGRFLLLATGCTAAALAVAQPTPAIVAPPWRAPVLLVEG
                     AAQPVRLRTLGVEVTVAAGVAETRVRMEFFNPNSRTLEGKLQFPLGAGQVVTGFALDV
                     DGELRDAVPVEKARAEQVFEDITRRRADPGLLQKTVGNNYELRIYPLPPGKTRTVVLK
                     LVEPAGDRLMLPLAYAARVERLDLSLRYPGVTTRPAFVGASPQGLAFVADRQGGFTAR
                     VSVANATLARWPLVVRAARTSAPGATTQSFGDETYFAVDLPISQRVAPRRLPKHVQIV
                     WDASASAANRNIDDELALLDAYFRKAQTLGVTLVRVADVAATPERFTVRDGDWSALRR
                     ALLATVYDGASNLGAVHHDGVSSEALWFTDGLANYGAPWHIAFPVPVYAVSSSVGSDE
                     AALQALAEASGGRRIDLLSSTRAQAAAALLEQGTRLVDIEAVGARGIVVETHRPAAGR
                     LQIAGVLTAGEAELTLRLRNADGTAWNRPLRIRAGENAAPLAAWQWARLTLASLEGEP
                     RLHKARIRELGQRFGLATPETSLLVLERVEDYVQHGIEPPPALRPAYDRLAGAAKQQV
                     AANDARRLADVVRRFDARVAWWNRDFPKDAPQAPLAVANAAPRAAVGTALASRRDVRI
                     AESEANRQPSPSPSMSLAAAANSAAPAVEAGTRPAAQKRREAGNPGAGADADAATPAS
                     ISIALKPALANAPWMRRLEAAEPASRLAVYLDERRANATSVAFFLDCADFFLAKGQRE
                     MGLRVLSNLAEMDLQNRQVLRLLGYRLQQAGETNLAVPIFERVLELAPYEPQSHRDLG
                     LALAAAGRPQAAVDRLYEVVTGAWDARFADIDLIALAELNAIVARSERDGKPVNVAAI
                     DPRLLKNMPLDVRVVLAWDADATDVDLHVIDPNGEEVFYGHNLSYQGGTISHDATGGY
                     GPEEFSLRHAKPGKYRIEANFFGHRQQILTSSTGLMMWLSSGFATKD"
BASE COUNT          510 a         1357 c         1303 g          541 t
ORIGIN      
        1 gaccttcttc gccaggctct gcaggatggc gagcttggtc gagaccttgc tgccggcgac
       61 gatggccacg agcggccgcc tcggatcggc cagcgccgtc gtgatcgcgt cgacttcagc
      121 ggcgagcagc gggccggcga cggcgaccgg cgcgaattcg gcgatgccgt aggtcgtcgc
      181 ctcggcgcga tgcgcggtgc cgaacgcgtc gttgacatag acgtcgcaga gcgccgccat
      241 cttcctggcg agcgcggcgt cgttcttctt ctcgcccttg tggaggcgac agttctcgag
      301 cacgacgagg gagcccggcg cgacctcgac gccgtccagc cagtcgcgcc gcagcggcgc
      361 cggccggccg agcaactggc cgaggcgctc ggcgaccggc gccagcgtgt cgcgcggctt
      421 ggcgtctccc tcggtcggcc ggccgaggtg cgacgtcacc atcacggcgg cgccggcgcc
      481 cagcgcctgc tcgatgcacg gcagcgaagc gcggatgcgg gtgtcttcgg tgatgcgccc
      541 gtcctcgtcc tgcggaacgt tgagatcggc gcggatgaag acgcgcttgc ccgcgaggcg
      601 cccgctgtcg acgaggtcgg tgaaacgcgg aagggtcatt cgcgggagca gacggcgggc
      661 cggggcaaag gggcgatttt aggcgccgcg ggtcggcgcg accgctgtcg gcgcctctgc
      721 ggcgccgcgc ggcggcagcg tttcaccccg ttcggccgtg ttgcccgtgt catgcgcagg
      781 agcggctcgc gccgctttcc accctccgcg acttccaggg cgaatgccat gcttgcacga
      841 ctcttcggac gattccttct tctcgcgacg ggctgtaccg cggccgcact cgccgttgcc
      901 cagccgactc ccgcgatcgt ggcgccgccc tggcgtgcgc cggttctgct cgtcgagggg
      961 gcggcgcagc cggtccgcct gcgcacgctc ggcgtcgagg tgacggtcgc ggcgggcgtc
     1021 gccgagacgc gggtgcggat ggagttcttc aacccgaact cccgcacgct cgaaggcaag
     1081 ctgcagttcc cgctcggcgc cggccaggtc gtgaccggat tcgcgctcga tgtcgacggc
     1141 gagctgcgcg acgccgtgcc ggtcgagaag gcgcgcgccg agcaggtgtt cgaggacatc
     1201 acccgccgcc gcgccgatcc gggattgctg cagaagacgg tcggcaacaa ctacgagctg
     1261 cgcatctatc cgctgcctcc cggcaagacg cgcaccgtcg tgctgaagct cgtcgagccg
     1321 gcaggcgatc gcctgatgtt gccgctggcc tacgccgcgc gcgtcgagcg gctcgatctc
     1381 tcgcttcgct atcccggtgt gacgacgcga ccggccttcg tcggtgcgtc gccgcaaggt
     1441 ctcgccttcg tggccgatcg ccagggcggc ttcacggcac gcgtgtcggt ggcgaacgcg
     1501 acgctggccc gctggccact cgtcgttcgc gccgcgcgga caagcgcgcc gggcgcaacg
     1561 acgcaatcct tcggcgatga aacctatttc gccgtcgacc tgccgatctc gcagcgcgtc
     1621 gcgccccgcc gcctgccgaa gcacgtgcag atcgtctggg acgcctcggc ctcggcggcg
     1681 aaccggaaca tcgacgacga gctcgccttg ctggatgcct atttccgcaa ggcgcagacg
     1741 ctcggcgtca cgctggtccg ggtggccgac gtcgccgcca cgcccgagcg attcaccgtc
     1801 cgcgacggcg actggtcggc gttgcgacgc gccttgctgg cgacggtcta cgacggcgcc
     1861 agcaacctgg gcgccgtcca ccacgacggc gtgtcttcgg aggcgctctg gttcaccgac
     1921 ggcctggcca actacggcgc gccgtggcac atcgccttcc cggtgccggt ctatgcggtg
     1981 tcgagcagcg tcggcagcga cgaggcggcg ctgcaggcgc tcgccgaggc cagcggcggc
     2041 cgccgcatcg acctgctctc gtcgacgcgg gcgcaagccg cggcggcttt gctcgaacaa
     2101 ggcacgcggc tcgtcgatat cgaagccgtc ggcgcccgcg gcatcgtcgt cgaaacgcac
     2161 cggcccgccg ctggacggct gcagatcgcc ggcgtcctga ccgcgggcga ggcggagctg
     2221 acgctgcgcc tgcgcaacgc cgacggcacg gcatggaacc ggcctctgcg catccgcgcc
     2281 ggcgagaacg cggcgccgct ggcggcgtgg cagtgggcac gcctcaccct cgcgtcgctg
     2341 gaaggcgagc cgcgcctgca caaggcccgg attcgcgagc tgggccagcg ctttggcctg
     2401 gcgacgcccg agacctcgct gctggttctc gagcgcgtcg aagattacgt gcagcacggc
     2461 atcgagccgc cgccggcatt gcgccccgcc tacgaccggc tcgctggcgc ggcgaagcag
     2521 caggtggcgg cgaacgatgc ccgtcgcctc gccgacgtgg tgcgccggtt cgatgcccgc
     2581 gtcgcctggt ggaaccgcga ctttccgaag gacgcgccgc aggcgccgct cgccgtcgcg
     2641 aacgccgcac cccgcgccgc ggtcggcact gcgctcgcgt cgcgacgcga cgtacggatc
     2701 gccgagtcgg aggcgaaccg ccaacccagc ccgtcgccgt cgatgtcgct cgcggcggca
     2761 gcgaattccg cggcgcctgc cgtcgaggcc ggcacgcggc ccgccgcgca aaagcgcagg
     2821 gaagccggca atcccggcgc cggcgccgat gccgatgccg cgacgccggc cagcatctcg
     2881 atcgctctga agccggcgct cgccaatgcc ccgtggatgc gacgtctcga ggcggccgag
     2941 cccgcgtccc gcctggccgt ctatctcgac gagcgccgcg ccaacgcgac gagcgtcgcc
     3001 ttcttcctcg attgcgccga cttcttcctg gccaagggcc agcgcgagat gggcctgcgc
     3061 gttctttcca acctcgccga gatggacctg cagaaccggc aggtgcttcg cctgctcggc
     3121 taccggctgc aacaggccgg cgaaacgaat ctggcggtgc cgatcttcga acgcgtgctc
     3181 gagctggcgc cctacgagcc gcagtcgcat cgcgacctcg gcctggcact ggccgccgcc
     3241 ggacggccgc aggccgccgt cgaccggctg tacgaggtcg tcaccggcgc gtgggacgcg
     3301 cgcttcgccg acatcgacct gatcgccctg gccgagctca acgccatcgt cgccaggtcc
     3361 gagcgagacg gcaagccggt gaacgtcgcc gcgatcgacc cgcgcctgct gaagaacatg
     3421 ccgctcgacg ttcgcgtcgt cctggcctgg gatgccgacg cgaccgacgt cgacctgcac
     3481 gtgatcgatc cgaacggcga ggaggtgttc tacggccaca acctgtctta ccagggcggc
     3541 accatcagtc acgacgcgac cggcggctac gggcccgagg agttctcgct gcgccacgcc
     3601 aagcccggca agtaccgcat cgaggcgaac ttcttcggcc accgccagca gatcctgacg
     3661 tcgagcaccg gcctcatgat gtggctgtcg agcgggttcg cgacgaaaga c
//
LOCUS       DASRWS010000350         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3023749, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000350 DASRWS010000000
VERSION     DASRWS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023749"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFF72_06600"
     CDS             complement(<1..514)
                     /locus_tag="VFF72_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92870.1"
                     /translation="MSPSTRIAGRRGIVPVVALLVACALPFFLGNYHLFQLTLVLVYA
                     IALLGLNMLTGFNGQISLGHGAFYAIGAYTAAILMDKAGMPYWATIPIAGLVCGIAGF
                     LFGLPALRLEGLYLALATFALGVAMPQLLKFHHLEKWTGGVQGIVIPKPEAPLGLPVN
                     ADQWLYFFTLF"
     gene            complement(528..1397)
                     /locus_tag="VFF72_06605"
     CDS             complement(528..1397)
                     /locus_tag="VFF72_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV92871.1"
                     /translation="MNLLLHQVLSGLATGGIYASVALALVMIYQATHLVNFAQGELAM
                     FCTYIAWALIQAGVPYWGAFALTVVGAFVLGVVIERVIVRPVENSPILAIVIVFIALL
                     VIFNSLAGWIFTYTIKPFPSPFPTQPLFGNSYMSSQEIGSMAITLVVLLLLFVFFRFT
                     PLGLAMRAAAENPVSSRLVGIRVGWMLALGWGLAAAIGSIAGMMVAPTVFLEPNMMGG
                     ILLYAFAGALLGGIDSPGGAVLGGFIVGVLENVVGAYVGSEIKLTVALVVIIGVLVVR
                     PSGLFGQVHVNRV"
     gene            complement(1412..2128)
                     /locus_tag="VFF72_06610"
     CDS             complement(1412..2128)
                     /locus_tag="VFF72_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92872.1"
                     /translation="MTTPLLEVRGLRARYGATQVLHGIDFAVATGGITTILGANGAGK
                     TTLLRAVCNMVRTEGEVVLAGTRIDARATESIVRLGVAHVPDGRGTFMHLSVEENLRL
                     GAHTRRDRAGVAADCERMYAHFPRLKERRRQQAGTLSGGEQQMLAVSRALMLRPRLLL
                     LDEPSFGLAPQIVQHLFEILRSIHDSEGIGMLLVEQNASLALQLADHAYLLETGRVVV
                     SGPASAIRGDESVRRSYLGY"
     gene            complement(2169..2903)
                     /locus_tag="VFF72_06615"
     CDS             complement(2169..2903)
                     /locus_tag="VFF72_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92873.1"
                     /translation="MRFGGITALDAVSFDVARGHVVGFIGPNGAGKTTMFNCLSRLYA
                     FSAGSIRFEGRSLLETPTHGIAALGIGRTFQNLALFRSMSVRANIMLGGHARTSTGWL
                     ANALRLPSVAREEGQLAALADRLIGLLDLGAVADAPVTDLPFGTQKRVELGRALASAP
                     KLLLLDEPAGGLNHEEVEALMELLRRIRAELGLTMLLVEHHMNMVMRISDRVVALDFG
                     RKIADGTPTEVQENADVIRAYLGTTA"
     gene            complement(3147..>3710)
                     /locus_tag="VFF72_06620"
     CDS             complement(3147..>3710)
                     /locus_tag="VFF72_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZV92874.1"
                     /translation="FGVTLEKVSKDERRTAKVINFGLIYGMSAFGLAANLGIERSAAI
                     AYIERYFARYPGVKRYMDETRASARDRGFVETLFGRRVYLPDIKAGGGPRRAAAERQA
                     INAPMQGTAADVVKLAMLAVQEALDREGRTTRMIMQVHDELVFEVPEAELGWAREAVP
                     RIMASVASLKVPLVAEVGVGANWDEAH"
BASE COUNT          639 a         1261 c         1297 g          513 t
ORIGIN      
        1 cgaacaaggt gaagaagtag agccactggt ccgcattcac aggtagaccg agcggagcct
       61 ccggtttcgg gatcacgatg ccctgcacgc cgccggtcca tttctccagg tggtggaact
      121 tcagcagctg cggcatcgcc acgccgagcg cgaaggtggc gagcgcgagg tagagccctt
      181 ccaggcgcag cgccggcagc ccgaagagaa agccggcgat gccgcagacg aggccggcga
      241 tcggaatcgt cgcccagtac ggcatccccg ccttgtccat caggatggcc gccgtgtacg
      301 cgccgatcgc gtagaacgcg ccgtggccga gcgagatctg gccgttgaag ccggtcagca
      361 tgttcaggcc cagcagcgcg atcgcgtaga ccagcaccag cgtgagctgg aagaggtgat
      421 agttgccgag gaagaacggc agcgcgcagg cgacgaggag ggccacgacc ggcacgatgc
      481 cgcgacggcc ggcgattcgt gtggacgggc tcatcggcgg ccgaccttca tacgcggttc
      541 acgtggacct ggccgaacag ccccgacggc cggacgacca gcacgccgat gatgacgacg
      601 agggccacgg tcaacttgat ctcgctgccg acataggcgc cgacgacgtt ctcgaggacg
      661 ccgacgatga agccgccgag gaccgcgccg ccggggctgt cgatgccgcc gagcagggcg
      721 ccggcgaatg cgtagaggag gatgccgccc atcatgttcg gctcgaggaa gaccgtcggc
      781 gcgaccatca tcccggcgat cgagccgatc gccgccgcga gcccccagcc gagggccagc
      841 atccagccga cgcggatgcc gacgagccgg ctcgagaccg ggttctccgc cgccgcgcgc
      901 atcgccagcc cgagcggcgt gaagcggaag aagacaaaca gcagcagcaa gacgaccagc
      961 gtgatcgcca tcgagccgat ctcctgcgac gacatatacg agttgccgaa cagcggctga
     1021 gtcgggaagg ggctcgggaa cggcttgatc gtgtacgtga agatccagcc ggccaggctg
     1081 ttgaagatca ccagcaaggc gatgaagacg atgacgatcg ccaggatcgg cgagttctcg
     1141 accggcctca cgatcacgcg ctcgatgacg acgccgagga cgaaggcgcc caccacggtg
     1201 agggcgaacg cgccccagta cggcacgccg gcctggatca gtgcccaggc gatgtaggtg
     1261 cagaacatcg ccagctcgcc ttgcgcgaaa ttcacgagat gggtggcctg gtagatcatg
     1321 accagcgcca gcgcgacgct ggcgtagatg ccgccggtcg ccagcccgga aagaacctgg
     1381 tgcaggagaa ggttcacgcc gacgtccccg ctcagtagcc gagataggag cgacgcaccg
     1441 attcgtcgcc gcggatcgcc gacgccggcc ccgagacgac gacccggccg gtctcgagca
     1501 ggtaggcgtg gtcggcgagc tgcagcgcca gcgaggcgtt ctgctcgacg agcagcatgc
     1561 cgatgccctc gctgtcgtgg atcgaccgga ggatctcgaa caggtgctgg acgatctgcg
     1621 gcgccagccc gaacgacggc tcgtcgagta gcagcaggcg cggcctgagc atcagcgcgc
     1681 gcgacaccgc gagcatctgc tgctcgccgc ccgacaacgt gccggcctgc tggcgacgcc
     1741 tctccttcag gcgcgggaag tgcgcgtaca tgcgttcgca gtcggccgcg acgccggccc
     1801 gatcgcgccg cgtgtgtgct cccaggcgca ggttctcctc gacgctcagg tgcatgaagg
     1861 tgccgcggcc atcgggcaca tgcgccacgc cgagccgcac gatcgactcg gtcgcgcggg
     1921 cgtcgatgcg ggtcccggcc aggacgacct cgccctcggt gcgaaccatg ttgcagacgg
     1981 cgcgcagcag cgtcgtcttg ccggcgccgt tggcgccgag gatcgtcgtg atgccgcccg
     2041 tggcgaccgc gaagtcgatg ccgtgcagca cctgcgtcgc gccgtagcgg gcgcgcaggc
     2101 cgcgcacctc gagcagcggc gtggtcatcg ctgcgcctcg cgccacgagg caggcgttcc
     2161 gtcgcgcctc atgccgtcgt cccgagatag gcgcggatga cgtcggcgtt ttcctgcacc
     2221 tcggtcgggg tgccgtcggc gatcttgcgc ccgaagtcga gggcgacgac acgatcggaa
     2281 atgcgcatca ccatgttcat gtgatgctcg acgagcagca tcgtcagccc gagctcggcg
     2341 cgaatgcgcc gcaggagctc catcaacgcc tcgacttcct cgtggttgag gccgcccgcc
     2401 ggctcgtcga ggagcagcag cttgggcgcg gaggcgagcg cgcggccgag ctcgacgcgc
     2461 ttttgcgtgc cgaacggcag gtcggtgacc ggcgcgtcgg cgacggcgcc gagatcgagc
     2521 agcccgatca ggcgatcggc aagagccgcc agctgccctt cctcgcgagc gacgctcggc
     2581 agccgcagcg cattggccag ccagccggtg ctcgttcgtg cgtggccgcc gagcatgatg
     2641 ttcgcgcgca cgctcatcga gcgaaagagc gccaggttct ggaaggtgcg gccgatgccg
     2701 agcgcggcga tgccgtgcgt cggtgtctcg agcagcgatc gaccctcgaa gcggatgctg
     2761 cccgccgaga atgcatacag acgcgacagg cagttgaaca tcgtcgtctt gccggcaccg
     2821 ttggggccga tgaagccgac gacgtggccg cgcgccacgt cgaaagagac ggcgtcgagc
     2881 gccgtgatgc cgccgaagcg cacggagaca ttctcgacct gaagaagcgg tgcagcgatg
     2941 ccttgccccc ggtgcgagag cctcgatgcc cgcaccttag ggaccggagg gccgaatgga
     3001 aggggtcttg ccctagttga cgccgcgagg cggcggacca tgggagccgg acttcgaatc
     3061 gcacccagcg cccccagcgc cgggcacgtc cgcttgcacg gcgacccgcg gctcgcccgg
     3121 tcgcgcgcga ccgggccggt caccgctcag tgcgcttcgt cccagttggc gccgacaccg
     3181 acctcggcga ccaggggcac cttgagcgag gcgacactgg ccatgatgcg cggcaccgct
     3241 tcccgggccc agccgagctc ggcctcgggc acctcgaaaa cgagctcgtc gtgcacctgc
     3301 atgatcatcc gcgtcgtccg tccttcacgg tccagcgcct cctggacggc cagcatcgcc
     3361 agcttgacga cgtcggcggc ggtgccctgc atcggcgcat tgatcgcctg gcgctcggcg
     3421 gcggcgcgac gcgggccacc gccggccttg atgtcgggca ggtagacccg ccggccgaac
     3481 agcgtctcga cgaagcccct gtcgcgcgcc gaggcgcgcg tctcgtccat gtagcgcttg
     3541 acgcccggat agcgcgcgaa gtagcgctcg atgtaggcga tggcggcgct gcgctcgatg
     3601 ccgaggttgg cggccaggcc gaacgcgctc atgccgtaga tcaggccgaa gttgatcacc
     3661 ttcgcggtgc gccgctcgtc cttgctgact ttctccagcg tcacaccgaa
//
LOCUS       DASRWS010000351         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2156844, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000351 DASRWS010000000
VERSION     DASRWS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156844"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..944
                     /gene="yihA"
                     /locus_tag="VFF72_06625"
     CDS             222..944
                     /gene="yihA"
                     /locus_tag="VFF72_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZV92875.1"
                     /translation="MTDDLPAAPTPFRKVEPGEGEEAKRALAWTHTARFLVTASQLSE
                     LPPSELPEIAFVGRSNAGKSTAINALAQQRRLAFASKTPGRTQHINLFELGPKDSPDA
                     LFADLPGYGYAAVERAAKQRWQQVMAEYLETRRNLETIVLLVDARLGITPIDRALLDF
                     VAPRIGNGTVRLLVLLTKTDKLSRQQTATALADAGKTLESIATEAADVSLVPFSALRG
                     QGVGDVAAFLRAVVRPLRSGVR"
     gene            complement(930..1436)
                     /locus_tag="VFF72_06630"
     CDS             complement(930..1436)
                     /locus_tag="VFF72_06630"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HZV92876.1"
                     /translation="MASSLNAFMQISNARGESKQGHYKDWIEIQAWEWEVEAETSWTK
                     GGGASVGKPSPNKMSWEHYWDRSSATLLVYICTGASFENIQLDMCKTTGSGTPQTFFS
                     IKMKDAFITKVNQSANEEGNVVQKVEMVFKEITIEYFQQGLDPKNPGALKQATKFYWD
                     IPGGSSHA"
     gene            1625..2524
                     /locus_tag="VFF72_06635"
     CDS             1625..2524
                     /locus_tag="VFF72_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV92877.1"
                     /translation="MIETRMIDLPGGIRLNCRSTGSKGAPVLLFLHGFPEAAFAWDAA
                     LEHFGKRFHCIAPNLRGYEGSSSPDEVEAYRARHLMADIAGLIETQGGRVEALVAHDW
                     GGALAWGLAAQRPSAMRRLVIVNSPHPATFLRALQGNPAQQAASAYMNFLSRPDAPAL
                     LAENDYARMWPFFEKMGAADESRPGGGWLTESMRQRYREAWGHGLRGPCNYYRASPLR
                     PATANDTKVMAIRFAPEDVTVKVPTLVVWGDADIALPASLLDGLDEFVPDMRISRIPG
                     ATHWIIHEHPALVMAEIEEALRR"
     gene            complement(2558..>2779)
                     /locus_tag="VFF72_06640"
     CDS             complement(2558..>2779)
                     /locus_tag="VFF72_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92878.1"
                     /translation="LQGRWSRVQLLWRSDRSLFFLFAGESPNHTHSITRRALERLSTA
                     GLVQPIESKPLVQRAVERMMRELSTRAAA"
BASE COUNT          484 a          894 c          886 g          515 t
ORIGIN      
        1 cacgcacacg ttggccgaga tggtggcacc cttcgcaggg tctgccgcgg ctggcgcgga
       61 ggcagcgggc gccgttgcgg ccgctggctc cgccgcgttg gcgaccgacg tgccgagaaa
      121 agccacgacc aggcagaaca ggactcgagc ggcaggcttc atggaaaagg caagtcctcg
      181 tgcaaaagcc gctgatttta caatgagcgt ttgccttgcc catgactgac gacctgccgg
      241 ccgctccgac gccttttcga aaggtcgaac caggcgaggg ggaagaagcc aaacgggcat
      301 tggcgtggac gcataccgca cggtttctcg tcacggcgag ccagctcagt gagctgcctc
      361 cgtcggagtt gccggaaatc gccttcgtcg gccgctccaa tgcggggaag tcgaccgcaa
      421 tcaacgccct ggcccaacag aggcggctcg ccttcgcctc gaagacgccc ggccgcactc
      481 aacacatcaa tctcttcgaa ctcggcccga aggattctcc cgacgccctg ttcgccgatc
      541 ttccgggata tggctacgcg gcggtcgagc gggcagccaa gcagcgatgg caacaagtga
      601 tggcggagta tctcgagact aggcgcaacc tcgagacgat cgtgcttctc gtcgacgccc
      661 ggctcggcat cacgccgatc gaccgcgccc tgctcgattt cgtggcgccg cgaatcggca
      721 acggcaccgt caggttgctc gtcctcctga ccaagaccga caagctttcg aggcagcaga
      781 cggcgacggc actcgccgac gccggcaaga ctctggagtc gatcgcgacc gaggccgcgg
      841 atgtgtcgct cgtgccgttt tcggccctgc gcggccaagg cgtcggcgat gtcgccgcct
      901 tcctgcgcgc cgtcgtccgg ccgcttcggt caggcgtgcg atgagccgcc ggggatgtcc
      961 cagtagaact tggtcgcttg cttcagggcg ccgggattct tcgggtccag gccttgctgg
     1021 aaatattcga tcgtgatctc cttgaagacc atctcgacct tctgcacgac gttgccttcc
     1081 tcgttggcgc tctggttcac cttggtgatg aacgcgtctt tcatcttgat cgagaagaag
     1141 gtctgcggcg tgcccgagcc ggtggttttg cacatgtcga gctggatgtt ctcgaacgat
     1201 gcgccagtgc agatgtagac cagcaacgtg gccgaagacc tgtcccagta gtgctcccaa
     1261 ctcatcttgt tggggctcgg cttgccgaca ctggcgccgc cgccctttgt ccaactcgtc
     1321 tccgcctcga cttcccactc ccaggcttgg atctcgatcc aatctttgta gtgcccttgc
     1381 ttggactccc cgcgggcatt cgagatctgc atgaaggcat tcagtgatga ggccatcgat
     1441 agctccctga tttggtatgg acagttgaga gattggctgg ccggtgtgcc gcaggtggcg
     1501 gcgccagaca agcgcgaaag cttgccaatc ggccgaacgc ttgtcaaatc cctccgggag
     1561 gtgagaacga tggacaagcg ccgtcatgga atacttcgtc gcaaccttcg cttggagatc
     1621 ccggatgatc gagaccagga tgatcgacct gccgggcggc atccgcctga actgccgatc
     1681 gaccgggtca aaaggggcgc cggttctcct tttcctgcat ggtttcccgg aagccgcgtt
     1741 cgcgtgggac gccgcgctcg agcacttcgg caaacggttc cactgcatcg cgccgaatct
     1801 tcgcggctac gaagggtcgt cgtccccgga cgaggtggaa gcctaccgtg ccaggcactt
     1861 gatggcagac atcgccggtc tcatcgagac ccagggtggc cgggtcgagg cactcgtcgc
     1921 gcacgattgg ggcggtgccc tggcttgggg ccttgccgcg caacggccgt cggcgatgcg
     1981 tcgcctggtg atcgtcaatt cgccgcatcc ggcgacgttt cttcgcgctc tgcagggcaa
     2041 tccggcacag caggccgcga gcgcctacat gaactttctc tctcggccgg acgcgccagc
     2101 gttgctcgcc gagaacgact acgcgcggat gtggcccttc ttcgaaaaga tgggcgctgc
     2161 cgacgaatcg cgcccgggcg gagggtggct gaccgaatcc atgcggcagc gctaccgcga
     2221 ggcatggggc catggcctgc gcgggccatg caactactac cgggcgtcgc cgctgcgtcc
     2281 ggcgactgcg aacgacacga aggtgatggc gattcgattc gcgcccgaag acgtgacggt
     2341 gaaggtgccg acgctcgtcg tctggggtga cgccgacatc gccctgccgg catcgcttct
     2401 cgacggcctc gatgaattcg tgcccgacat gcgcatttca cgcattcccg gcgcgacgca
     2461 ctggatcatt cacgagcacc ctgcgcttgt catggccgaa atcgaagaag cgcttcgtcg
     2521 ctaacgagcg ccagcaacga gtcggacagg cgccggatta cgccgctgcc cgcgtcgaca
     2581 gctcgcgcat catccgttcg acggcccgct ggaccagcgg cttggattcg atgggctgca
     2641 cgaggcccgc ggtgctgagc cgctccagcg cccgacgcgt gatcgagtgg gtgtggttcg
     2701 gcgattcgcc ggcgaaaagg aagaacaggc tgcggtcgct tcgccaaagc aactgaacac
     2761 ggctccaacg gccttgcag
//
LOCUS       DASRWS010000352         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1593484, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000352 DASRWS010000000
VERSION     DASRWS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593484"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..719
                     /locus_tag="VFF72_06645"
     CDS             24..719
                     /locus_tag="VFF72_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV92879.1"
                     /translation="MLELSGLHAYYGKSHILHGVDMQVGEGEIVALLGRNGSGRSTTV
                     KTIMGLVHGEGSIAWRGQEILGRKAFDIAHLGIGYVPENRDIFPTLTVEQNLQLGEKR
                     GAARPRWSFEDMYGMFPRLRERRHTEAGVLSGGEQQMLTLCRTLMGDPDLIMIDEPTE
                     GLAPKIVELVASFLQTLKTRGVSVLLVEQKLTIALEISQRCYVMGHGAIVFDGTPEAL
                     RGNAEVRKEWLEV"
     gene            832..>2184
                     /locus_tag="VFF72_06650"
     CDS             832..>2184
                     /locus_tag="VFF72_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZV92880.1"
                     /translation="MSASYEVRGDVAVITMNNPPVNGLGYDTRRGIVEGLDRALVDAA
                     VKAIVITGAGKAFSGGADIREFGSPKAIAEPNLLSVILALEASSKPIVAAVHSVAMGG
                     GLELALGCHYRVAAAGTQVALPEVKIGLIPGAGGTQRLPRVLGVETALNMIVSGEPVK
                     SELLARQPGQKLFDQVVEGDALEAAIAFAREHADRRPLPLVRDLKIVYPNAEAYFQFA
                     RNTVGAMSRNFPAPLKCIDAVAASMKMKFDDGMRYERELFMALMLTPECKALRHVFMA
                     ERAASKIADVPADTPQRQIRKVAVIGAGTMGGGIAMNFLNAGIPVVMLEMKQEALDKG
                     TGIMRKNYEAQVKKGKLKQDKLDERMALLTTTLKYDDLKDADLVIEAVFEDMAVKEQV
                     FKTLDEVMKPGAILASNTSTLNVDTIAAFTKRPEDVVGMHFFSPANVMKLLEVVRGAK
                     T"
BASE COUNT          395 a          717 c          740 g          332 t
ORIGIN      
        1 gaaaggaagg ggctggcgcc gtgatactcg agctgagcgg cctgcacgcc tactacggca
       61 agagccacat cctgcacggc gtcgacatgc aggtcggcga gggcgagatc gtcgcgctgc
      121 tcggccgcaa cggctccggc cggtcgacga cggtgaagac gatcatgggc ctggtgcacg
      181 gcgagggctc catcgcgtgg cgcggccagg agatcctcgg ccgcaaggcg ttcgacatcg
      241 cccatctcgg catcggctac gtgccggaaa accgcgacat ctttccgacg ctgactgtcg
      301 agcagaacct ccagctcggc gagaagcgcg gcgcggccag accgcggtgg agcttcgagg
      361 acatgtacgg catgtttccg cggctgcgcg agcgccgcca taccgaggcc ggcgtcctct
      421 cgggaggcga gcagcagatg ctgacgctgt gccgcacgct gatgggcgac cccgatctga
      481 tcatgatcga cgagccgacc gaggggctgg cgcctaagat cgtcgagctc gtcgcctcct
      541 tcctgcagac gctcaagacg cgcggagttt cggtgctcct cgtcgagcag aagctcacca
      601 tcgccctcga gatctcgcag cgctgctacg tcatggggca cggtgccatc gtcttcgacg
      661 gcacgcccga ggcgcttcgc ggcaacgccg aggtgcgcaa ggagtggctc gaggtctgag
      721 cccgttgtcg ctgctgggac agcgatgtcg tggggcggcg acctagaatc gaacgaacgt
      781 gctttttccc gcgccgcacg gcgcgaactg ctttcccccg ggggttgtcc catgagcgcc
      841 agctacgagg tccgcggcga cgtcgccgtc atcacgatga acaacccgcc tgtcaacggg
      901 ctgggctacg acacccgtcg cggcatcgtc gaggggctcg acagggcgct ggtcgatgca
      961 gccgtcaagg cgatcgtcat cacgggcgcc ggcaaggcgt tctcgggcgg cgccgacatc
     1021 cgcgaattcg gctcgccgaa ggcgattgcc gagccgaacc tcctctccgt catcctggcg
     1081 ctcgaggcca gcagcaagcc gatcgtcgcc gccgtgcaca gcgtggcgat gggcggcggt
     1141 ctcgaactgg ccctcggctg ccactaccgg gtggctgcgg ccggcacgca agtggcgctg
     1201 ccggaggtga agatcggcct cattcccggt gccggcggca cccagcgcct gccgcgcgtg
     1261 ctcggcgtcg agaccgcgct caacatgatc gtcagcggcg agccggtgaa gagcgaactg
     1321 ctggccaggc agcccgggca gaagctcttc gaccaggtcg tggagggcga cgcgctcgaa
     1381 gcggccatcg ccttcgcgcg cgagcacgcc gaccggcggc cgctgcctct ggtgcgtgac
     1441 ctgaagattg tctatccgaa cgccgaggct tacttccagt tcgcgcgcaa cacggtcggc
     1501 gcgatgtcgc gcaacttccc ggcgccgttg aagtgcatcg atgccgtcgc cgcgtcgatg
     1561 aagatgaagt tcgacgacgg catgagatac gagcgcgagc tgttcatggc gctcatgctg
     1621 acgcccgagt gcaaggcgct gcggcacgtc ttcatggccg agcgtgccgc cagcaagatc
     1681 gccgacgtgc cggccgacac gccgcagcgc cagatcagga aagtcgcggt gatcggcgcc
     1741 ggaacgatgg gcggcggcat cgccatgaac ttcctcaatg cgggcatccc ggtcgtcatg
     1801 ctcgagatga agcaggaggc cctcgacaag ggcaccggca tcatgcgcaa gaactacgaa
     1861 gcgcaggtga aaaagggcaa gctcaagcag gacaagctcg acgagcggat ggcgctgctg
     1921 acgacgacgc tgaaatacga cgatctgaag gatgccgacc tcgtcatcga ggccgtgttc
     1981 gaggacatgg ccgtcaagga gcaggtcttc aagacgctcg acgaggtgat gaagcccggc
     2041 gccatcctcg ccagcaacac ctccaccctc aacgtcgaca ccatcgccgc gttcacgaag
     2101 cggcccgagg acgtcgtcgg catgcacttc ttcagtcccg ccaacgtcat gaagctgctc
     2161 gaggtggttc gcggcgcgaa gacg
//
LOCUS       DASRWS010000353         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1593493, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000353 DASRWS010000000
VERSION     DASRWS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593493"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFF72_06655"
     CDS             <1..232
                     /locus_tag="VFF72_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HZV92881.1"
                     /translation="SFQWNPESQQTLENWQSEFLQLLWQVGGLAYFLYLGSPQSREGD
                     ERKEAKLDALLRHLGPEGERIVTELDERYPRR"
     gene            425..742
                     /locus_tag="VFF72_06660"
     CDS             425..742
                     /locus_tag="VFF72_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92882.1"
                     /translation="MNTHDEKVRDKLHDTLTRANVDTRNLAIEVVDGRLIVKGTVPST
                     DQQEQMKVLLRHCADQGLSLECDVSVLKAAPSDSLDGRGRSPITGTSPDSAHESRHQL
                     DRS"
     gene            complement(1429..3387)
                     /locus_tag="VFF72_06665"
     CDS             complement(1429..3387)
                     /locus_tag="VFF72_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZV92883.1"
                     /translation="MASERGEHEVQLDLLIDAVVDYAIYMIDPEGYVLSWNTGAERLK
                     GYSRAEILGQHFSRFYVPEEQAAGVPKRALETARSEGRFAAEGWRIRKDGSRFWASVV
                     IDAIKDSRGRLVGFAKVTRDITERQEAQAGLLESERRYRRLVEAVVDYAIFQLNIDGR
                     IATWNVGAQRIKGYSADEAIGRHFSMFYTEEDRAAGVPARALAIAGAEGRFEAEGWRV
                     RKDGSRFWALVVIDAIYDDHRKLVGFAKVTRDITERYEAQQALRETQIQLAASQKMEA
                     VGQLSGGIAHDFNNLLMIVLGNLEMAQRQVRQMPEATNLQRYVANAIRGAQRASALTS
                     RLLAFSRRQALDPKPIDVNKFIAGSSDFLQRSLGETIEIEAVGGAGIWPIEADPNQLE
                     SALVNLAINARDAMPGGGKLTIEAANVFADDDYVRLNPEVHEGQYVAICVSDTGSGMS
                     KEVAARAFEPFFTTKEAGHGTGLGLSQIYGFVKQSGGHVKIYSEPGQGTTVKMYFPRL
                     LQRTIEQEAEEALPVAEGESETILVVEDDADVRAYLLEMLRELKYRVIPADNAQAALR
                     VLVDDSRRVDLLMTDVIMPGLNGRELGRRAQALRPGLSVLYMTGYSRNAVVHQGRLDE
                     GVDLIQKPISEAQLATRIRQILDRKACP"
     gene            complement(3910..4122)
                     /locus_tag="VFF72_06670"
     CDS             complement(3910..4122)
                     /locus_tag="VFF72_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92884.1"
                     /translation="MTAIKTNVPAQQRARAEEARRKAENEANKTTQKALLNDAALWER
                     MADYEEKHPQPEEQHCQSDEQRPRSK"
     gene            complement(4281..>4498)
                     /locus_tag="VFF72_06675"
     CDS             complement(4281..>4498)
                     /locus_tag="VFF72_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92885.1"
                     /translation="YPLGVECRACGHKGLIPAERFGGCKGDMTQLERLRLACMATPSA
                     LRSGGRSVSCAQGARGRQNLIPVALPR"
BASE COUNT          777 a         1440 c         1409 g          872 t
ORIGIN      
        1 cagcttccag tggaatcctg aatctcaaca gaccctagag aactggcaat cggagttcct
       61 gcagctcctt tggcaagtcg gaggcttggc ctactttctc tatttaggct ctccgcagtc
      121 gcgcgaagga gacgagcgca aagaggccaa gcttgatgca ctccttcggc atcttgggcc
      181 tgaaggcgag cggatcgtaa ccgagctgga tgagcggtac ccgcgtcgtt gaatcccagg
      241 ctcggaaatt cctttgctgc tcggcttacc agcctcgttc ctgagtgtcc aacgttcgcg
      301 ggactcactc gatggtgcaa ttggcatgcc gccctgcgcc acgccgcagg cgccacctgc
      361 ggcagcaaca ggcgccacgc tctgatcgtt gaacttaaga accaacggga taccggagaa
      421 acccatgaat acgcacgacg agaaagttcg agacaagttg catgacaccc tgacacgggc
      481 gaacgtcgat acgcgcaact tggcgattga ggtagtggat ggccgtttga tcgtgaaggg
      541 aaccgtaccc tcaactgacc agcaggaaca gatgaaggtg ctgcttcgcc attgcgcgga
      601 ccaagggcta tcgctggagt gcgacgtatc ggtcctcaag gcggcgccaa gcgacagctt
      661 ggatggccgc ggccgctcgc cgataacggg cacctcgccg gactcggcgc acgaaagtcg
      721 ccatcagctc gatcgctctt agcacggcca ggggcctcgc ggggcgccta gtacggatgg
      781 gcctcgatgc ctcacctatt ggcttatgcc gcggataggt tatagcccgt cgcgtggctc
      841 caggcctatt tctcgcagcc gatcggctga tgctgctcca gcatgcacgc tcggcgaagc
      901 aggcgccttg ctgcctcgca tcacggagag ccttggacga gcgggcgtgg tcggcgcatg
      961 cactccaaag acctagcggc cgttggtccg gcttcttacc gcgcttagcg ccgtgacaac
     1021 gactctacct cgagatggaa gtgccgacgc tgaagacgcg ggccgaccga tgtcgaaatc
     1081 acggcccctc ctcgccgagc actagcgata tcccgatgac ctggaaagcg ccgtcgcctg
     1141 atattggcct gaccacatca actggcacct cgggctcctc ggaccattcc cacagatgca
     1201 tcccacattg cggcacgttt gctcggagga ttagagcgaa aacgccgacg gcttttcgca
     1261 gctccggtgc tcggcacttt cgctacatga gcgcgagtgc acctgggccg gctctcccaa
     1321 gccggattcg aagtgatgct ggaaactata tcgagcaccg cgaccggttg gtacgagaat
     1381 ttgccgaatc cgataagtcg cggacgtccg aggggcgagc ccagatcgct aggggcatgc
     1441 ctttctgtcc agtatctgcc ggattcgggt cgcgagctgg gcctcgctga tcggcttctg
     1501 gatcaggtcg acgccctcgt cgagccggcc ctggtggact acggcgttgc gcgagtagcc
     1561 ggtcatgtac agcaccgaca ggccgggccg cagcgcctgc gcccggcggc cgagctcgcg
     1621 gccgttgagg ccgggcatga tcacgtcagt catcagcagg tcgacccgcc ggctgtcgtc
     1681 taccaggaca cgcagcgcgg cctgcgcgtt gtcggccgga atcacccggt acttcagttc
     1741 gcgcagcatc tccagcaggt aggcgcgcac gtcggcatcg tcctcgacca ccaggatcgt
     1801 ctcgctctcg ccctcggcga cgggcagggc ctcctcggcc tcctgctcga tcgtgcgctg
     1861 cagtagccgc gggaagtaca tcttgaccgt cgtgccctgg cccggctcgc tgtagatctt
     1921 cacgtggccg cccgactgct tgacgaagcc gtagatctgg ctcaggccga ggccggtgcc
     1981 gtggccagcc tctttggtgg tgaagaacgg ctcgaacgcg cgcgccgcga cctccttcga
     2041 catgccgctg ccggtgtcgc tgacgcagat cgcgacgtac tggccctcgt ggacctcggg
     2101 attgaggcgg acatagtcgt cgtcggcgaa gacgttggct gcctcgatcg tgagcttgcc
     2161 gccgcccggc atggcgtcgc gcgcgttgat cgcgaggttt accagggccg attcgagttg
     2221 gttcgggtca gcctcgatcg gccagatgcc ggcgccgccc accgcctcga tctcgatggt
     2281 ctcgccgagc gagcgctgca ggaagtcgct cgagccggcg atgaacttgt tgacgtcgat
     2341 cggcttgggg tcgagcgcct ggcggcgcga gaaggcgagc agccggctgg tgagtgccga
     2401 cgcccgctgc gcgccgcgaa tcgcattggc gacatagcgc tgcaggttgg tcgcttccgg
     2461 catctgccgg acctggcgct gggccatctc gagattgccc agcacgatca tcagcaggtt
     2521 gttgaaatcg tgggcgatgc cgccgcttaa ctggccgacc gcctccatct tctgcgacgc
     2581 tgccagttga atctgcgtct cgcgcagcgc ttgttgcgcc tcgtagcgtt cggtgatgtc
     2641 gcgcgtcact ttggcgaagc cgaccagctt gcggtggtcg tcatagatcg cgtcgatcac
     2701 gaccagcgcc cagaagcggc tgccgtcctt gcggacgcgc cagccctcgg cctcgaaccg
     2761 gccttcggcg ccggcgattg caagcgcgcg agccggcacg ccggcagcgc ggtcctcctc
     2821 ggtatagaac atcgagaaat gccgaccgat cgcctcgtcg gcgctgtagc ccttgatgcg
     2881 ctgcgcgccg acgttccagg tggcgatccg tccgtcgata ttcagctgga agatggcata
     2941 gtcgaccacc gcctcgacga ggcggcgata gcgccgctcg ctctccagca ggccggcctg
     3001 cgcctcctgc cgctccgtaa tgtctcgcgt caccttggcg aagccgacca gtcgtccccg
     3061 tgaatctttg attgcatcga ttacgactga cgcccagaag cgactgccgt ccttgcggat
     3121 ccgccatcct tccgctgcga agcggccttc gctgcgcgcg gtctcgaggg cgcgtttggg
     3181 tactccagcc gcctgctcct caggcacata gaatcgggag aaatgctggc ctaggatttc
     3241 ggcgcggcta taccccttca agcgctccgc gccggtgttc cagctcagta catacccctc
     3301 cggatcgatc atgtagatcg catagtcaac tactgcatcg atcaacagat cgagctggac
     3361 ctcgtgctcg ccgcgttcag acgccatttt tcgctgtctc gccgtggatc acctgaacca
     3421 acggcggctg tacagtaccg ttcccggaag ccaacagcga ccggaaggcg cgagacgccg
     3481 cagggccttg tcctgacaac gcacagtggc tgggcatccc tttgtagcct aagatgcgtc
     3541 tgggtgatgt ggtaagctag tgggtctcct gcatcgcgta aaatgccact ggccgacgaa
     3601 gtggggaggt gcagccccac cacatccgat agtcccggcg attattgaaa cactactgca
     3661 aacacagctg caattccttt cgtcctcatc catgcgttaa acccccgaaa cgacacgccg
     3721 tccgtgatgc gacgcagcgg cgaggacggc catgggcgcg ccatagatgc cgtcgagtgc
     3781 cacgaaagct tacagggcgc ttaggtcagc cacgatggtc gtcgaatgcg acggggatag
     3841 tcgcgatcct tccggctaca tggatgcccg tcggcatcga gggatgccta cggtcgaggg
     3901 gagtcgcggt cacttggacc gcgggcgctg ttcgtcggat tgacaatgct gctcttcggg
     3961 ttgggggtgc ttttcctcgt agtcggccat ccgttcccag agcgcggcat cgttgagcag
     4021 cgctttctgg gtagttttgt tggcctcgtt ttcggccttg cggcgggcct cctctgcccg
     4081 cgcgcgttgc tgagctggca cgttggtctt aatcgcggtc atggcagttc acttatccga
     4141 cggtggaaac gtcgcgccgc tgttttggta acactatggc tggaaaaatt tgcgacctcg
     4201 cgtgggcaac tacgaagcat tttgcgcggc tagcccgagc cggcatctgc tggtcgtcct
     4261 ggggctgggc ccccagacct tcagcgtgga agggccacgg ggatcagatt ctggcggcct
     4321 ctcgcccctt gcgcgcacga aacggaacgg ccacctgatc ttagcgcgct tggcgtggcc
     4381 atgcaggcca ggcgaagacg ctccagctga gtcatgtcgc ccttgcagcc gccgaagcgc
     4441 tcggccggta tcagaccttt gtgaccgcag gcgcggcatt cgacgcccag cgggtaca
//
LOCUS       DASRWS010000354         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3025528, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000354 DASRWS010000000
VERSION     DASRWS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3025528"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFF72_06680"
     CDS             complement(<1..394)
                     /locus_tag="VFF72_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92886.1"
                     /translation="MTNTNMRLGPRLRAAAAVAMLAAAGMAAQAQDSGGSAGPAPAAG
                     AEPGTTATPAGATPWYLGASESLTRDSNVYRVPGGPGDTYSSTSLLGGFDQHIGRQRV
                     FGNANVGINRYDGQSRLNNTSYNVAGGLD"
     gene            complement(444..1826)
                     /locus_tag="VFF72_06685"
     CDS             complement(444..1826)
                     /locus_tag="VFF72_06685"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HZV92887.1"
                     /translation="MFDDPHKRTFFSAPQSVTSLIAALLEPGITIGVFIAATLASDEP
                     MDRPSLTLCLLVLALTFPGRNRFRDSLLGAAVDIVSSWLVLLAVLALCAYATDSFHYF
                     DNNILLIWVLATPVLQWLATVIGKLVVRRNAERSRRTAVVVGAGPLGVKVAKALEAGL
                     QGVECLGYFDDRADGRVDDAARSQLLGSLADVADFTTAKGVRDVYITLPLGSQPRIQQ
                     LLEQLQGTTASLFYVPDVFGISIIQGRLQDLAGVPVVGICETPFTGTNQLVKRASDLI
                     LASIILILISPLLVAIAIGVKMSSPGPVIFRQRRNGLDGDEIVVWKFRSMSTQDDGAV
                     VQQATRNDPRVTRFGAFLRASSLDELPQFFNVLQGRMSIVGPRPHAVTHNEQYRQIIR
                     AYMVRHKVKPGITGWAQVNGQRGETDTIEKMRARVEYDLEYLRNWSLALDLRIIAGTI
                     RLVFFDRYAY"
     gene            complement(1896..2576)
                     /locus_tag="VFF72_06690"
     CDS             complement(1896..2576)
                     /locus_tag="VFF72_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV92888.1"
                     /translation="MTHILLLEDIPEAAAWLKVQIAQVFDDAVFHDASRVHEALALIN
                     KVQFDVGLIDLGLPDGSGVDVVAALHSAHPEAQAVVVTIHDDDDHLFPALQAGAFGYV
                     LKDQQAPMIVDQFRRMSQGEPPLSPAIARRVMAHFASRSSQRERPKHMPSVVLTDREN
                     EVLLRVAKGFTLPEIAAQLNLSRYTISDYVKQIYRKLNVSSRAEAALEAQRLGLFGRG
                     RRAQPTAN"
     gene            complement(2841..>4650)
                     /locus_tag="VFF72_06695"
     CDS             complement(2841..>4650)
                     /locus_tag="VFF72_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV92889.1"
                     /translation="TLLALGVAAGCAQAAAPPPDAILRIDHAWAVAGSDGRFPGNRPA
                     DIVPLPDDWSVSRPGYEGSVWYRIAFSVNRPNLTDELAAIYVERACSNLQVLLNGQLI
                     FVGGRMVEPVTRNCSRPQLVTLPPALLRAKDNIVDIRVEGHALERVASRENAAGLSVI
                     EIGNQAELRRLHAPRVLWGVTWVDASSLALIGLGCVLLAVGWLNRREVYFFYFGWLTL
                     GWVAWSLIDSARDLPWSNEVTEFLLCASWSVMLALALQFFLTFAGRRSRAIESLSALQ
                     WVVMPVSLVVAGPSRLFDVARLWYALLALELVGVMGIYLAITFRQRRQDFLPMLVVVA
                     ISIVLLGLELGVQAGWVARPAVSAPEVIVPLLLVAVGTRLFLMFARALRTTQDDRNRI
                     AGELHRLNAEIESRVEHLTVERVGQFTEMERRRIASDLHDDLGAKLLTIVHTTDSTRM
                     PQLAREALEEMRLSVRGLAGKAVRLDDAIADWRAETMVRLDQSHIAGKWDGVAMADPP
                     TLSARVHMQLTRVLREAVSNVIKHSGATQCQVRCAVEGGSLVVTLRDNGRGIDGDINT
                     GNGMSTMKRRAKGMNGQCLVESRPGHGVVISLTVPL"
BASE COUNT          752 a         1539 c         1659 g          700 t
ORIGIN      
        1 agtcaaggcc gccggcgacg ttgtagctcg tgttgttcag gcggctctgc ccatcatagc
       61 ggttgatgcc gacgttggcg ttgccgaaga cgcgctgacg cccgatgtgc tggtcgaagc
      121 cgccgagcag gctggtactc gagtaggtgt cgcccgggcc tccgggcacg cgatagacgt
      181 tggagtcgcg ggtaagcgac tcgctggcgc cgagatacca cggcgtcgcg ccggccggcg
      241 tcgcggtcgt gccgggctcg gcgcccgcgg ccggcgccgg tccggcgctg cctccgctgt
      301 cttgagcctg cgccgccatg cccgccgcgg cgagcatggc aaccgcggcg gcggcccgca
      361 gccgggggcc gaggcgcatg ttcgtattgg tcatcgcatt cctccgatct gcgccgcatt
      421 gtcgggccgc gacccggccg ggctcaatac gcatagcgat cgaagaacac cagccggatc
      481 gtccccgcga tgatccgcag atcgagggcg agcgaccagt tgcgcaggta ctcgaggtcg
      541 tattcgacgc gggcgcgcat tttttcgatc gtgtcggttt cgccgcgctg gccgttcacc
      601 tgcgcccagc cggtgatgcc gggcttgacc ttgtggcgaa ccatgtaggc gcggatgatc
      661 tggcggtact gctcgttgtg cgtcaccgcg tgcggccgcg gcccgacgat gctcatgcgg
      721 ccctgcagca cgttgaagaa ctgcggcagc tcgtcgagcg agctcgcacg caggaaggcg
      781 ccgaagcggg tcacgcgtgg gtcgttgcga gtcgcctgct gcaccaccgc gccgtcgtct
      841 tgagtcgaca tcgagcggaa cttccacacc acgatctcgt cgccgtcgag gccgttgcgg
      901 cgctggcgaa agatgaccgg gccgggcgag ctcatcttca cgccgatggc gatcgccacc
      961 agcagcggcg agatcaggat caggatgatc gaggcgagga tgaggtcgct cgcccgcttg
     1021 acgagctggt tggtgcccgt gaagggcgtt tcgcagatgc ccaccacggg cacgccggcg
     1081 aggtcttgca gccgcccctg gatgatgctg atgccgaaca cgtcgggcac gtagaagagc
     1141 gaggcggtgg ttccctgcag ctgctcgagg agctgctgga tgcgcggctg cgacccgagg
     1201 ggcagggtga tgtagacgtc gcgaacgcct tttgccgtcg tgaaatcggc gacgtcggcc
     1261 aggctgccga gcagctggct gcgggcggcg tcgtcgaccc ggccgtcggc gcggtcgtcg
     1321 aaatagccga ggcactcgac gccctgcagg ccggcctcga gcgccttcgc caccttcacg
     1381 ccgagcggcc ccgcacctac caccaccgcg gtgcggcgcg atcgctccgc gttgcggcgg
     1441 acgacgagct tgccgatcac cgtcgccagc cactgcagca ccggcgtcgc gagcacccag
     1501 atcagcagga tgttgttgtc gaagtagtga aagctgtcgg tggcgtacgc gcacagcgcc
     1561 agcacggcga gcagcaccag ccacgacgag acgatgtcga cggcggcgcc gaggaggctg
     1621 tcacgaaagc ggttgcggcc cgggaaggtg agtgccagca ccagcaggca gagcgtcagc
     1681 gacggccggt ccatcggctc gtcggacgcc agcgtggccg cgatgaacac cccgatcgtg
     1741 atgcccggct cgaggagcgc ggcgatcagc gacgtgaccg attgcggggc gctgaaaaag
     1801 gttcgcttgt gcggatcgtc gaacatcggc aggttcgccc gtcgcggcga gctgcgagaa
     1861 gaagcaggcc cggatcatgc cccgggttcg gaggatcagt tggcggtggg ctgggcccgg
     1921 cggccgcggc cgaacaggcc gagcctttgc gcctcgagcg ctgcttcggc gcgcgagctc
     1981 acgttgagtt ttcggtagat ctgtttgacg tagtcggaga ttgtgtatcg cgacaggttg
     2041 agctgcgccg cgatctcggg cagcgtgaag cccttggcga cgcgcagcag cacctcgttc
     2101 tcgcggtcgg tgaggacgac gctcggcatg tgcttgggcc gctcgcgctg cgaggagcgc
     2161 gacgcgaagt gcgccatcac gcgccgggcg atcgccggcg acaggggcgg ctcgccctgg
     2221 ctcatgcgcc ggaactggtc gacgatcatc ggcgcctgct ggtccttcag gacgtagccg
     2281 aacgcgccgg cctgcaaggc cggaaagaga tgatcgtcgt cgtcgtggat ggtgacgacg
     2341 accgcctgcg cctcgggatg agcgctgtgc agcgcggcga cgacgtcgac acccgacccg
     2401 tcgggcaggc cgagatcgat caggccgacg tcgaattgca ccttgttgat cagcgccagc
     2461 gcttcgtgga cgcgcgatgc gtcgtggaag acggcgtcgt cgaacacctg cgcgatctgc
     2521 accttgagcc aggcagccgc ctccggaatg tcttcgagca gcaggatgtg ggtcatggcg
     2581 agagctccag cggcgcgttc atggggaacc gacagacgcc cccgcgccgt ctgggggaat
     2641 ctctttggca aaaggcgtgc cgatgccact gcgagacgcg ggggcatcga tcgactgtgc
     2701 ggcgcgcaga ctctcgaggc caagcccgtg gcggcgcagc ggtcgatccg gcgcgacgaa
     2761 tgcgaaaaac gttcgctcgc gccgggcgat ccgtccgatc ctagccgcca ggggggccat
     2821 ttccagccgc cctgtcggct tcacagcgga accgtgagcg atatcacgac gccatggccg
     2881 ggccgcgact cgaccaggca ctgcccgttc atgcccttgg cgcgccgctt catcgtcgac
     2941 atgccgttgc cggtgttgat gtcgccgtcg atgcctcgac cgttgtcgcg caaggtcacg
     3001 acgagcgagc cgccctcgac ggcgcaacgc acctggcact gcgtcgcgcc gctgtgcttg
     3061 atgacgttgc tcaccgcctc gcgcaggacc cgcgtcagct gcatgtgcac gcgcgccgaa
     3121 agcgtcggcg gatcggccat ggcgacgccg tcccacttgc cggcgatgtg cgactggtcg
     3181 agccgcacca tcgtctcggc gcgccagtcg gcgatcgcgt cgtcgaggcg caccgccttg
     3241 ccggcgaggc cgcgcaccga aaggcgcatt tcctcgagcg cttcccgggc gagttgcggc
     3301 attcgcgtgc tgtcggtggt gtgcacgatc gtcagcagct tggcgccgag gtcgtcgtgc
     3361 aggtcggagg cgatccgtcg ccgctccatt tcggtgaact ggccgacccg ctcgaccgtg
     3421 agatgctcga cgcggctctc gatctcggcg ttgagccggt gcaattcgcc ggcgatccgg
     3481 ttgcgatcgt cctgggtggt gcgaagggcg cgggcgaaca tcaggaaaag gcgcgtgccg
     3541 acggccacga gcagcaaggg aacgatcact tcgggtgccg agacggccgg ccgcgcgacc
     3601 catccggcct gcacaccgag ctccaggccg aggagcacga tcgatatggc gacgacgacg
     3661 agcatcggca ggaagtcctg gcggcgctgc cggaacgtga tggccaggta gatgcccatc
     3721 acgcccacca gctccagtgc cagcagggcg taccagagac gggcgacgtc gaacaaccgc
     3781 gacgggccgg cgacgacgag cgagaccggc atcaccaccc attgcagtgc gctcaggctc
     3841 tcgatggccc gcgatcttcg gccggcgaag gtgaggaaga actgcagcgc cagggcgagc
     3901 atcaccgacc acgatgcgca gagcaggaac tccgtcacct cgttcgacca cggcaggtcg
     3961 cgcgcggagt cgatgagcga ccaggccacc cagccgagcg tcagccagcc gaaatagaag
     4021 aagtacacct cgcgccggtt cagccagccg acggcgagaa gaacgcagcc caggccgatc
     4081 agcgccaggc tgctcgcatc gacccaggtc acgccccaga ggacgcgcgg cgcgtgcagg
     4141 cggcgaagct cggcctggtt gccgatctcg atgaccgaaa gcccggcggc gttctcgcgc
     4201 gaggcgactc gctcgagcgc atgaccttcg acccggatgt cgacgatgtt gtccttggca
     4261 cgcagcaagg ccggcggcag cgtcaccagc tgcggccgcg agcagttgcg cgtcaccggc
     4321 tcgaccatcc ggccgcccac gaagatcagc tggccgttga ggagcacctg caggttgctg
     4381 caggcgcgct cgacgtagat cgccgccagc tcgtcggtga ggttcggccg gttgacgctg
     4441 aaggcgatcc ggtaccagac gctgccctcg taaccgggcc gactcaccga ccagtcgtcg
     4501 ggcagcggga cgatgtcggc cgggcggttg cccgggaagc gaccatccga gccggcgacg
     4561 gcccaggcgt gatcgatgcg caggatggcg tcaggcggcg gcgcggccgc ctgcgcgcag
     4621 cccgccgcga cgccgagggc cagcagcgtg
//
LOCUS       DASRWS010000355         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4171824, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000355 DASRWS010000000
VERSION     DASRWS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4171824"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFF72_06700"
     CDS             <1..290
                     /locus_tag="VFF72_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HZV92890.1"
                     /translation="LSDSVPELVEQALRNGGPKCDNVSVLAVEFEGVEGDSQLGGIQT
                     AALGEAVFASTIQASVLGSDVSPDELDDAEIERSIKEINEAIKRSSQQKKG"
     gene            451..1185
                     /gene="rph"
                     /locus_tag="VFF72_06705"
     CDS             451..1185
                     /gene="rph"
                     /locus_tag="VFF72_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZV92891.1"
                     /translation="MTFQRSSGRAHDALRPVSITRSFTRHAEGSVLVAFGDTKVLCTA
                     SVEEKVPPHKRGSGEGWLTAEYGMLPRSTHTRSDREAARGKQSGRTQEIQRLIGRSMR
                     CVFDLSRLGERTISLDCDVLQADGGTRTAAITGAFVAAHDAVAWLLASGRIEHSPIRD
                     FVAAVSVGIVQGVPLLDLEYVEDSACDTDMNVVMTGSGGFVELQGTAEGATFSRTEMD
                     ALLALAGKGIGELVDLQRRSLGLLSA"
     gene            1203..1859
                     /gene="rdgB"
                     /locus_tag="VFF72_06710"
     CDS             1203..1859
                     /gene="rdgB"
                     /locus_tag="VFF72_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZV92892.1"
                     /translation="MRLVLAAGNPGKLVELQSLLAPLDLEIVAQSSLGIDEAEEPYAT
                     FLENALAKARHAAAAAGAAAIADDSGLVVDALGGAPGVASAHFAPIEMGSLTDREERR
                     RHQDSANNALLLERLAGRDDRGARFVSTLVAVRHAGDPEPLVAFGRWPGEIARTPRGK
                     NGFGYDPLVYITALGRTVAELDAGTKNSASHRALAARDMLALMRSAWQLGSASSPRAP
                     "
     gene            1856..>2228
                     /locus_tag="VFF72_06715"
     CDS             1856..>2228
                     /locus_tag="VFF72_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZV92893.1"
                     /translation="MSGPREPVDAPVDHAKPRREEVSRPGEASVAGRLANLQGPGAIS
                     LAALPPLSLYVHLPWCIRKCPYCDFNSHAIRGRGKDDLPQQRYVEALVADLDAALPFV
                     WGRRVHTVFIGGGTPSLFAPAQ"
BASE COUNT          338 a          782 c          768 g          340 t
ORIGIN      
        1 cgctctcgga ctcggtgccc gagctcgtcg agcaggcgct gcgcaacggc ggcccgaagt
       61 gcgacaacgt gtcggtgctc gcggtcgagt tcgaaggcgt cgagggcgac agccagctcg
      121 gcggcatcca gaccgcggcg ctcggcgagg cggtgttcgc ttcgacgatc caggcgagcg
      181 tgctcggcag cgatgtgtcg ccggacgagc tcgacgacgc cgagatcgag cggtcgatca
      241 aggaaatcaa cgaagcgata aaaagatcgt cccagcagaa gaaagggtga ttgcaccgtc
      301 gagcgggacg aactttttac agcttcgcct caggattgct ccctacctgc ttcgctcccc
      361 tccctccggg agaaaggggc ggccttcttc gaaggccctt tcggtggctc ggctcggtcg
      421 aggtgaggcg atgcggcgag aatcgtgcgc atgacattcc aacgttccag cggccgcgcc
      481 cacgatgcgc tccggcctgt ctcgatcacc cgctctttca cccgccacgc cgagggctcg
      541 gtcctcgtcg ccttcggcga caccaaggtg ctgtgcaccg cctcggtcga ggagaaggtg
      601 ccgccgcaca agcgcggctc gggcgagggc tggctgacag ccgaatacgg catgctgccg
      661 cgctccaccc acacgcgcag cgaccgtgag gcggcgcgtg gcaagcagag cgggcgcacc
      721 caggaaatcc agcggttgat cggccgctcg atgcgatgcg tgttcgacct ctcgcggctc
      781 ggcgagcgga cgatctcgct cgactgcgac gtcctgcagg ccgacggcgg cacgcgaacg
      841 gcggccatca ccggcgcctt cgtcgctgcc cacgacgccg tggcatggct gctcgccagt
      901 ggccggatcg agcattcgcc gatccgagat ttcgttgccg ccgtctcggt gggcatcgtc
      961 cagggcgtgc ctttgctcga tctcgagtac gtcgaggatt cggcctgcga taccgacatg
     1021 aacgtcgtca tgaccggctc cggcggattc gtcgaactgc agggaacggc cgagggcgcg
     1081 accttttcga ggacggagat ggacgcgctg ctcgcgctcg ccggcaaggg catcggcgag
     1141 ctcgtcgacc tgcagcggcg cagcctcggc ctgctttcgg cctgaaaaga cgggagcccg
     1201 ccgtgcggct cgttctcgcg gccggcaatc cgggcaagct ggtcgagctg cagagccttc
     1261 tcgcgccgct cgacctcgag atcgtcgccc agtcgagcct gggcatcgac gaggccgagg
     1321 agccatacgc gacatttctc gagaacgccc tggccaaggc gaggcacgcc gcggcggccg
     1381 ccggcgccgc cgccatcgcc gacgattccg gcctcgtcgt cgacgcgcta gggggcgcac
     1441 cgggtgtggc ttctgcgcac ttcgcgccga tcgagatggg ctcgctgacc gatcgcgagg
     1501 agcggcgacg ccaccaggac agcgccaaca acgccctctt gctcgagcgg ctggcgggtc
     1561 gcgacgatcg cggcgcgcgc ttcgtctcga cgctggtcgc ggttcgccat gccggcgatc
     1621 ccgagccgct cgtcgccttc gggcgatggc cgggcgagat cgcgcgcacg ccgcgcggca
     1681 agaacggttt cggctacgac ccgctggtct acatcacggc gctcggccgg accgtcgccg
     1741 agctcgatgc gggaacgaag aattcggcca gccaccgcgc cctcgctgcc cgcgacatgc
     1801 tcgcgctgat gcgcagtgca tggcagctcg gcagcgcatc ttcgccgcgg gcaccatgag
     1861 cggcccccgg gagccggtgg acgctcccgt cgaccacgcg aaaccgaggc gtgaagaggt
     1921 ctcccggccg ggcgaggcaa gcgttgccgg ccggctcgca aaccttcagg gtccgggcgc
     1981 gatatcgctg gcggcgctgc cgccgctgtc gctctacgtc cacctgccct ggtgcatccg
     2041 caagtgcccg tattgcgact tcaactcgca cgccatccgc gggcgcggca aagacgacct
     2101 gccgcagcag cgctacgtcg aggcgctcgt cgccgacctc gacgcggcgc tgcccttcgt
     2161 ctggggccgg cgcgtgcaca cggtcttcat cggcggcggc acgccgagcc tcttcgcgcc
     2221 ggcgcagg
//
LOCUS       DASRWS010000356         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3593821, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000356 DASRWS010000000
VERSION     DASRWS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3593821"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFF72_06720"
     CDS             complement(<1..314)
                     /locus_tag="VFF72_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZV92894.1"
                     /translation="MRTVRRLFYVDIVSAVAFVALAFLALFFFIDFVDELGGIGRDGF
                     TALGAAAYSLLLAPGHFYELMPIAVLIGCIYALARLAQSSQYTILRTGGLGPGRALWL
                     LT"
     gene            complement(311..1435)
                     /gene="lptF"
                     /locus_tag="VFF72_06725"
     CDS             complement(311..1435)
                     /gene="lptF"
                     /locus_tag="VFF72_06725"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HZV92895.1"
                     /translation="MLFYSTLRRDLARNFGGTLVVILTIVLTIMFIRTLGQAALGRVS
                     PQDVLLLLAYIGLGQLSTMLGLSLFITIVATLSRMYRGSEMTIWFASGVSLTRFLRPV
                     LRTSWPVLLVIALLALFAWPWQNRRSAELQDEYNRRSDLSRIAPGQFQTSADGKRTFF
                     FEGNPKDTSTGHNVLIVATKGDVESVTTARSGHIENDAGSRFVILDKGQRNEENTANG
                     EKTLSRFESYRAEAGDRAAEASRSNAPRARSTADLLRDPTPDNLGELVWRLGLVFAGI
                     NVVFLGVGLSASNPRHASNWNLLFALLGFFAYYNFINLSQSWVASGRVGAAAALFITH
                     GSAFVLAISLLWWRQHGTNRVVPPRRRRRRRRVAPRPDSA"
     gene            1464..2927
                     /locus_tag="VFF72_06730"
     CDS             1464..2927
                     /locus_tag="VFF72_06730"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZV92896.1"
                     /translation="MDFGHRVSPPASLADIAVDGLVLIVVADEGLDPAMAPAIADLVA
                     DAVAHGDLALKKGKSLDAWKPAGVAARRIVVTIAADASAKAFKAAVAPAFATLKRGAA
                     ESIAVAWGSASAWTDVHAEACVLALADATYLYRHSKPSAAAPWAPKSLTLHCRKGEGK
                     AVDLGLKRGAAVAEGVTLAREFGNRPGNQCTPSWLGQQAKKMGKSLGLDVDVLDRKAV
                     EKLGMGCFLAVAQGSDEPLRFIVARYDGAAKSTPPVVLVGKGITFDTGGISLKPGAEM
                     DEMKFDMCGAASVLGTLHAIATLKPRLNLVCIVPSCDNMPSGKAVKPGDVVTSMSGQT
                     VEILNTDAEGRLILCDALTYAERFKPAVVVDVATLTGACVIALGHHHSGLFTTDDAIA
                     ARMTAARQAALDPCWRMPLDDEYDEALKSNFADMGNVGGRPGGAITAAMFLRRYTAKY
                     PWAHLDIAGTAWKSGAAKGATGRPVGLLTHFVLSQAR"
     gene            2952..3383
                     /locus_tag="VFF72_06735"
     CDS             2952..3383
                     /locus_tag="VFF72_06735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZV92897.1"
                     /translation="MTEISFHFNVAAPVGYACRLVRKAARRGAGVVVTGPRPLLDELD
                     REIWTFDAAEFVAHAWAEDSAAVPGSLHESLVWLATDPLAAPAHDALVNLGAESPRGY
                     ESFARLIEIVATAEPDRAAARERWKSYARRGYAIERHEVGE"
     gene            4031..>4217
                     /locus_tag="VFF72_06740"
     CDS             4031..>4217
                     /locus_tag="VFF72_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92898.1"
                     /translation="MPPRPTPASLPASTASTGAPLGSDEAQTMRRVLSDLQRQVDLSL
                     EVRLRELLAPMLSRIADT"
BASE COUNT          652 a         1490 c         1482 g          593 t
ORIGIN      
        1 ctcgtcagca gccaaagcgc ccggcccggc cccagcccgc cggtgcgcag gatcgtgtat
       61 tgcgaagact gcgccagccg ggccaaggcg tagatgcagc cgatcagcac ggcaatcggc
      121 atcagctcgt agaagtggcc gggggccagc agcagcgagt aggcggccgc gcccagcgcc
      181 gtgaagccgt cgcggccgat gccgccgagc tcgtcgacga agtcgatgaa gaagaagagg
      241 gcgagaaagg ccagggcgac gaaggcgacg gccgaaacga tgtcgacgta gaacaggcgg
      301 cgaaccgtcc tcatgccgaa tccggccggg gcgccacgcg ccgtcgtcgc ctgcgccgcc
      361 gcggcggcac gacccggttg gtgccgtgct ggcgccacca cagcaggctg atcgccagca
      421 cgaaggccga gccgtgggtg atgaagagcg ccgcggcggc tccgaccctg ccggaggcca
      481 cccacgactg cgagaggttg atgaagttgt agtaggcgaa gaagccgagc agggcgaaaa
      541 gcaggttcca gttgctcgcg tgccgtggat tggacgccga caggccgacg ccgaggaaga
      601 cgacgttgat gcccgcgaag accaggccca ggcgccagac cagctcgccg aggttgtcgg
      661 gcgtggggtc gcgcagcagg tcggcggtcg agcgggcgcg cggcgcgttg cttcgcgacg
      721 cctcggcggc gcggtcgccc gcctccgccc ggtagctttc gaaccgcgac agcgtcttct
      781 cgccgttggc ggtgttctcc tcgttgcgct ggcccttgtc gagaatgacg aagcggctgc
      841 cggcatcgtt ctcgatgtga ccgctgcgcg ccgtcgtcac cgactcgacg tcgcccttgg
      901 tcgcgacgat cagcacgttg tggccggtcg atgtgtcctt cggattgcct tcgaagaaga
      961 aggtgcgctt gccgtctgcc gacgtctgga actgtccggg ggcgatccgc gaaagatcgg
     1021 agcggcggtt gtactcgtcc tgcagctcgg cgctgcgccg gttctgccac ggccacgcga
     1081 agagcgccag cagggcgatg acgagcagca ccggccagct ggtgcgcagc accggtcgca
     1141 ggaagcgcgt cagcgagacg ccgctggcga accagatcgt catctcgctg ccgcgataca
     1201 tgcgcgacag ggtcgcgacg atggtgatga agagcgacag cccgagcatc gtcgagagct
     1261 ggccgaggcc gatgtaggcc agcagcagca gcacgtcctg cggcgagacg cggccgagcg
     1321 cggcctggcc gagcgtgcgg atgaacatga tcgtcagcac gatggtcagg atgaccacga
     1381 gcgtgccgcc gaagttgcgc gccaagtctc gacgcagagt ggaatagaat aacatgatcg
     1441 gcttgcctac tgccggcgga attatggact tcggccatcg cgtctcacct cccgcaagcc
     1501 tcgccgacat cgcggtcgac gggctcgtgc tcatcgtcgt cgccgacgaa ggcctggacc
     1561 cggcgatggc gccggcgatc gccgacctcg tcgccgatgc cgtggcacac ggcgatctgg
     1621 cgttgaagaa gggcaagtcg ctcgacgcct ggaagcccgc cggcgtcgca gcccggcgaa
     1681 tcgtcgtgac gatcgctgcg gacgcgtcgg cgaaagcgtt caaggcggcc gtcgccccgg
     1741 cctttgcgac gctcaagcgc ggcgccgccg aaagcatcgc ggtcgcctgg ggcagtgcca
     1801 gcgcgtggac cgacgtccac gccgaggcct gcgtcctcgc cctggccgat gcgacctacc
     1861 tctatcgcca cagcaagccg agcgcggccg cgccctgggc cccgaagtcg ctgaccctgc
     1921 attgccgaaa aggcgaaggg aaggctgtcg atctcggcct gaagcgcggc gccgccgtcg
     1981 ccgaaggcgt gacactggcc cgggagttcg gcaatcggcc gggcaaccag tgcacgccga
     2041 gctggctcgg ccagcaggcg aaaaagatgg gcaagtcgct cggtctcgac gtcgacgtgc
     2101 tcgatcgaaa ggcggtcgag aagctcggca tgggctgttt tctcgccgtc gcccagggtt
     2161 cggacgagcc gctgcgcttc atcgtcgccc gctacgacgg tgccgcgaag tcgacaccgc
     2221 cggtggtcct ggtcggcaag ggcatcactt tcgacaccgg tggcatctcg ctcaagccgg
     2281 gcgccgagat ggacgagatg aagttcgaca tgtgcggggc cgcgagcgtg ctcggcacgt
     2341 tgcacgcgat cgccacgctc aagccccgcc tcaacctcgt ctgcatcgtt ccgagctgcg
     2401 acaacatgcc gagcggaaag gccgtcaagc cgggcgacgt ggtgacgagc atgtcgggcc
     2461 agacggtcga gatcctcaac accgacgccg aaggacgcct gatcctgtgc gacgcgctga
     2521 cgtacgcgga gcgcttcaag ccggcggtcg tcgtcgacgt ggcgacgctc accggcgcct
     2581 gcgtgatcgc cctcggtcat catcattcgg ggctcttcac caccgatgac gccattgccg
     2641 cgcgaatgac cgcggccaga caggcggcgc tcgatccgtg ctggaggatg ccgctcgacg
     2701 acgaatacga cgaggcgctg aagagcaact tcgccgacat gggcaacgtc ggcgggcggc
     2761 ccggcggcgc gatcaccgcg gcgatgttcc ttcgccgcta caccgccaag tacccctggg
     2821 cgcatctcga tatcgccggc acggcctgga agtcgggcgc ggccaagggc gcgacgggac
     2881 gaccggtcgg cctgttgacg cacttcgtgc tgtcgcaggc tcgctgaagc gcgcgctcga
     2941 tcgcggctcg aatgaccgag atcagcttcc atttcaacgt cgcggcaccg gtcggctatg
     3001 cgtgccggct ggtccgcaag gcggcgcggc ggggcgccgg tgtcgtcgtc acggggccgc
     3061 ggccgctgct cgacgagctc gaccgcgaga tctggacctt cgatgccgcc gagttcgtcg
     3121 cgcacgcgtg ggccgaagat tcggccgccg ttcccgggtc attgcacgag agcctggttt
     3181 ggctggcgac cgacccgctc gcggcgccgg cgcacgacgc cctcgtcaat ctcggcgccg
     3241 aatcgccccg gggctatgag tcgttcgcgc ggctgatcga gatcgtcgcc acggccgagc
     3301 cggaccgggc cgcggctcgc gaacggtgga agagctacgc ccggcgcggc tatgcgatcg
     3361 agcgccacga ggtcggcgaa tgagcaccgg caggccgatc ccgccggccg gcatcccgac
     3421 cttgaccgaa gtggtgacct ggcccgacgt gcatgcgccg accgccccgg tcgacgcgga
     3481 cgatgcgacg agcccggccg cctcccaggt ctcgcctgcg acgacttcgg ccggtcccgc
     3541 gacgtcgaca acgccggccg ccgcgaggtc ggccccgccc gcggcgccgg cacgtttcgg
     3601 cccgtcgaac ccggagcctc tcccgcagcc gacgccgccc actcgcgtcc tgctcgcccc
     3661 gcgggcaacc tcgcgacccg gcggatcggc ttcggcaccc gcacctgcag cggtgccggt
     3721 gccgcctgcg atccccgtgc cgggcgcgac aaccgtgccg gccgtgacag ccgtgcccag
     3781 cgcgacaccc gcgccggccg cgactcccgg ggcggttcct gcgcccgcgc cggccgcggc
     3841 atccaggccg gttgcggcgc ccaccccggc cgccgcgcca tcggtgcggg ccgtctcgcc
     3901 cgtgccttcc tcgcggtcga ctgccgcgcc cgcacctgac ccggctgcag cttccgctcg
     3961 tgcagactct gttccggctc cctcggcctc gacgccgggc gggccgcagc cgacggcggt
     4021 gtccgggtcg gtgccacccc gtccaacgcc ggcgtcgctg cccgcgtcga ctgcctcgac
     4081 cggcgccccg ctcggcagcg acgaggcgca gacgatgcgg cgggtgctct cggacctgca
     4141 gcgccaggtc gacctgtcgc tcgaggttcg gctgcgcgaa ctattggcgc cgatgctttc
     4201 gcgtatcgcc gacaccc
//
LOCUS       DASRWS010000357         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2158548, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000357 DASRWS010000000
VERSION     DASRWS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158548"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VFF72_06745"
     CDS             <1..1507
                     /locus_tag="VFF72_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HZV92899.1"
                     /translation="FYGAMDGASKFVRGDAVAGILILFINIVGGLAIGMLQHDMALSA
                     AANNYILLAIGDGLVAQIPALVISTAAGLVVSRVGKEQQTGSQLASQLTAQPQALVIA
                     ALVLGVVGLVPGMPHVAFLTLAAGMGALARALFRRQAKPAPAPVAAPIVPPEQRDVSW
                     DDLQPVDALGLEVGYRLIPLVDTGQDGELLRRIKAIRRKFAQDVGFLPPPVHIRDNLE
                     LKPSAYRLTLRGAIVGEGEAFPGMWLAINPGGTSTPLIGTPTTDPAFGLPATWIEERQ
                     REQAQMAGFTVVDCPTVIATHLSHLMQLHAARLLGRVETQQLVEHVTKLAPKLMEDVV
                     PKMVGIAALQKVLQLLLEEGVHIRDMRSIVECLAEFAATITDPAELARRIRVHLAPSI
                     VQQIYGPTKELEVIAMEPELERMVTQALNSPHGPALDPGVADTLSKSAAQSAKRQEDL
                     GHPACLLVPDMIRAPMARLLKRAAPRLKVLSHSEIPETHSIRIGSIIGATS"
     gene            1504..>2740
                     /gene="flhF"
                     /locus_tag="VFF72_06750"
     CDS             1504..>2740
                     /gene="flhF"
                     /locus_tag="VFF72_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HZV92900.1"
                     /translation="MTSSPMNVRRFTARTSREALRQVREQLGIDAVVLSTQPGGSGVE
                     VLAMAPETLREVEAMGADVPLASTSPVASPRRAADLARTSAADDASRLEMSTLSFQDY
                     VRTRMLRRRQAALEQAAAPAGETRQVPASRVDIRVEDDGGFDAERSASPGEIYAAAAA
                     ASAEREDGRGLGALEQRMAERASTRPSEGWREPTPSAEPIRVAASAAAASAYDAAEAQ
                     RERLEMMSELRSMKGLIEERFGALAFMEKLQRRPAEARLSQKLLDCGFSPALTRKIAE
                     SLGGDVADEIAWATQVLERNLLAQGGASTLEEVGGTYALIGATGVGKTTTTAKIAAAF
                     AARHGAANLGLVTLDAYRLGAHEQLRAYGRILGVPVHTAHDRVTLEDLLDLLSAKKMV
                     LIDTAGIGQRDARTRELLDM"
BASE COUNT          418 a         1000 c          937 g          385 t
ORIGIN      
        1 tttctacggt gcgatggacg gcgcgtccaa gttcgtgcgc ggcgacgcgg tcgcgggcat
       61 cctgatcctt ttcatcaaca tcgtcggcgg ccttgccatc ggcatgctgc agcacgacat
      121 ggcgctgtcg gccgctgcga acaactacat cctgctcgcg atcggcgatg ggctggtggc
      181 gcagattccg gcgctcgtga tctcgacggc cgcgggcctg gtcgtgagcc gcgtcggcaa
      241 ggagcagcag accggcagcc agctcgcgag ccagctgacg gcgcagccgc aggcgctggt
      301 gatcgcggcg ctcgtgctcg gcgtcgtcgg cctcgtgccc ggcatgccgc acgtggcgtt
      361 cctgacgctt gcggcgggga tgggcgcgct cgcgcgcgcg ctgttccgcc ggcaagcgaa
      421 gcccgcgccg gccccggtcg cggcgcccat cgtacccccc gagcagcgcg acgtgtcgtg
      481 ggacgacctg cagccggtcg atgcgctcgg cctcgaagtg ggataccggc tgatcccgct
      541 cgtggacacg gggcaggacg gcgagctcct gcgacgcatc aaggccatcc ggcgcaagtt
      601 cgcgcaggac gtcggcttcc tgccgccgcc ggtgcacatc cgcgacaacc tcgagctgaa
      661 accgagcgcc tatcgattga ccctgcgcgg tgccatcgtc ggcgaaggcg aggcctttcc
      721 cggcatgtgg ctggcgatca acccgggcgg cacgtcgacg ccgctgatcg gcacgccgac
      781 caccgatccg gcgttcggcc tgccggcgac ctggatcgag gagcgacagc gcgagcaggc
      841 ccagatggcc ggctttaccg tcgttgattg cccgaccgtc atcgccaccc acctctcaca
      901 cttgatgcaa ctgcacgccg cgcgcctgct cggccgcgtc gagacgcagc agctcgtcga
      961 gcacgtgacc aagctggcgc ccaaactcat ggaagacgtc gttccgaaga tggtcggcat
     1021 cgccgccctg cagaaggtgc tgcagctcct cctcgaggaa ggcgtgcaca tccgcgacat
     1081 gcgctcgatc gtcgagtgcc tggccgagtt cgccgcgacg atcaccgatc cggccgagct
     1141 ggcgcggcgc atccgcgtcc acctggcgcc ttcgatcgtc cagcagatct acgggccgac
     1201 caaggaactc gaggtgatcg ccatggagcc cgagctcgag cgtatggtca cccaggcgct
     1261 caactcgccg cacggcccgg ccctcgaccc gggcgtcgcc gacacgctca gcaagagtgc
     1321 ggcgcaatcg gccaagcgcc aggaagacct cggccatccg gcctgcctgc tcgtccccga
     1381 catgatccgc gcgccgatgg cgcgcctcct caagcgcgcc gcaccgcgcc tgaaggtgct
     1441 gtcgcatagc gagatccccg agacccattc catccgcatc ggctcgatca tcggagcaac
     1501 ctcatgactt ccagccccat gaacgtcagg cgattcaccg cgcgcacgtc gcgcgaagcg
     1561 ctgcgccagg ttcgcgagca gctcggcatc gatgccgtcg tcctgtcgac ccagcccggc
     1621 ggctccggag tcgaggtgct ggcgatggcg cccgagacgc tgcgcgaagt cgaggcgatg
     1681 ggcgccgatg tgccgctggc ctcgacctcg ccggtcgcgt cgccgcgccg cgccgccgat
     1741 ctggcccgga cttcggccgc cgacgacgcg tcgcgtctcg agatgagcac gctgtcgttc
     1801 caggattacg tgcgcacgcg catgctcagg cgccggcagg ccgcgctcga gcaggccgcc
     1861 gcgccggccg gcgagacccg ccaggtgccg gcgtcgcgtg tcgacatccg ggtcgaggac
     1921 gacggcggct tcgacgccga gcgctcggcc tcgcctggcg agatctatgc cgctgccgcc
     1981 gcggcgtcgg cagaacgcga agacggccgg ggcctcggcg ccctcgagca acgcatggcc
     2041 gagcgcgcgt cgacgcgtcc ttccgaaggc tggcgcgaac cgacgccctc ggccgagccg
     2101 atccgcgttg ccgcatcggc cgccgcggcg agcgcgtacg acgccgccga ggcacagcgc
     2161 gaaagactcg agatgatgag cgagcttcgc tcgatgaagg gcctgatcga ggagcgcttc
     2221 ggcgccctgg cgttcatgga aaagctgcag cgccggccgg ccgaggcacg gctgtcgcaa
     2281 aagcttctcg actgcggctt ttctcccgct ctcacgagga agatcgccga gagcctgggc
     2341 ggcgacgtcg ccgacgagat cgcctgggcg acccaggtgc tcgagcgcaa cctgctggcg
     2401 cagggcggcg catcgacgct cgaggaggtg ggcggcacgt atgcattgat cggcgccacc
     2461 ggcgtcggca agacgacgac gacggccaag atcgccgccg ccttcgcggc ccgccacggc
     2521 gcggccaacc tcggcctggt gacgctcgac gcgtaccgcc tcggcgcgca cgaacagctg
     2581 cgcgcctacg gccgcatcct cggcgtgccg gtgcacacgg cgcacgaccg cgtcacgctc
     2641 gaggacctgc tggacctgct cagcgccaag aagatggtgc tgatcgacac cgccggcatc
     2701 ggccagcgcg atgcgcgcac gcgcgagctg ctcgacatgc
//
LOCUS       DASRWS010000358         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2159436, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000358 DASRWS010000000
VERSION     DASRWS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159436"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="VFF72_06755"
     CDS             complement(<1..1873)
                     /locus_tag="VFF72_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HZV92901.1"
                     /translation="MASTTVAQLAAELNRPAAALLEQLKSAGVGKAGADDSVTETDKA
                     RLLDHLRSSHGTAPGAGERKKITLTRKSTSEIKQADSSGKARTIQVEVRKKRTFVKRD
                     EVAAAPVDEAAAAESHEAELRRREEEAHAEAEQLRVQEEELREQQRRREAQERAEREV
                     AELRAREAAAKLAAEAAAAAAAAGEATAGKPAPEKVADAKAPEGKGAESKAAEGKAAE
                     PGKPALRVVKAGTIENEEKQRAADLAKRRKAAEDEAAAIRTMMSTPKKVLVAKKAEEA
                     KPAEPAKEAIKGTIHKKAGTPGATTTTAAKPGDKKSIKSEKLSSSWADDAAKKRALKA
                     GPRGAPGAGRAGWRAPRAGRRGDRNDGAPSTFVAPAEFQAQEVHIPETISVADLAHKM
                     SVKASEVIKQLMKLGQMVTINQQLDQETAMIVVEEMGHTATAAKLDDPEAFLEDAETE
                     SNQPAESRAPVVTVMGHVDHGKTSLLDYIRTSRVAAGEAGGITQHIGAYHVETPRGMI
                     TFLDTPGHAAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAAKVPIVVAMN
                     KIDKPEANVERLKSELVDALARLCGDVREHGVAAPLLGLEPERGEVAHDLLGSAPGLS
                     "
     gene            complement(1882..3429)
                     /gene="nusA"
                     /locus_tag="VFF72_06760"
     CDS             complement(1882..3429)
                     /gene="nusA"
                     /locus_tag="VFF72_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZV92902.1"
                     /translation="MNRELLMLVDAISREKSVDLEIVFQAVEAALASASKKLQGGEVD
                     IRVSVDRETGEYETFRRWHVVPDEAGLQLPDSEVLHFEAVEQIADIEVDDYIEEQIES
                     VSIGRIGAQAAKQVILQKIRDAEREQLLTDFLSRGEKIFVGTVKRLDKGDIVVESGRI
                     EGRLRRSEMIPKENLRTGDRVRAYIAEVDPTLRGPQIILSRSSPGFMKELFKQEVPEM
                     EQGLLEIKSCARDPGSRAKIAVQSHDKRVDPIGTCVGVRGSRVNAVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVQSIVVDEERHAMDVVVDEENLAIAIGRGGQNVRLASEMT
                     GWRINIMTAEESASKQANESDSVRKLFVDKLDVDAEVADILIAEGFSSLEEVAYVPMQ
                     EMLEIESFDEDTVNELRARAKQALLTMELAQAENGGIVSQDLHDFVGLTPELIDKLNA
                     GGVHSRDELADLAVDELIEISGVDADVAKDLIMKAREHWFAAEAQAASSQDAPADSAT
                     QEAATQG"
     gene            complement(3426..4061)
                     /gene="rimP"
                     /locus_tag="VFF72_06765"
     CDS             complement(3426..4061)
                     /gene="rimP"
                     /locus_tag="VFF72_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZV92903.1"
                     /translation="MTQQAAIDTTVRGLGYELVDVEREAGGLLRVTIDHPAVAGRSRA
                     ITVDDCEKVTRQLQHVLEVEGLAYERLEVSSPGLDRPLRTAADFARFEGEAVELTLKE
                     AFQGRKRFRGELQARGDDWRLVLAPTAPAAAARRHAVSRSAQKRRAAAGEGDAPAAEG
                     PVKVLDFSLGEVREARLVPVIDFKGRRSAPQGAASAHEAGAGQEMDGGRIQ"
     gene            4136..>4543
                     /locus_tag="VFF72_06770"
     CDS             4136..>4543
                     /locus_tag="VFF72_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV92904.1"
                     /translation="MGRKIRPLTLSGKTGIVSFLAITGKQPPCQEARTASDRVPESAA
                     TPPFRSFSMGFLAGKRLLVTGLLSNRSIAYGIARACHREGAELALSYQGERFLPRVTE
                     FAAEFGSKLVFDCDVADDGQIERMFTGLGEAWPT"
BASE COUNT          605 a         1589 c         1499 g          850 t
ORIGIN      
        1 tggacaagcc cggcgctgat cccagaagat catgcgcgac ctcgcctcgc tcgggctcca
       61 acccgaggag tggggcggcg acaccatgtt cgcgcacgtc tccgcaaaga cgggcgaggg
      121 catcgaccag ctccgacttc agacgctcga cattggcctc cggcttgtcg atcttgttca
      181 tggcgacgac gatcggcacc ttcgccgcct tggcatgggc gatcgcctcc ttggtctgcg
      241 gcatcacgcc gtcatcggcg gcgacgacga ggatgacgat gtcggttgcc ttggcgccgc
      301 gcgcccgcat cgccgtgaag gcggcgtggc ccggggtatc gaggaaggtg atcatgccgc
      361 gcggcgtttc gacgtggtac gcgccgatgt gctgcgtgat gccgcctgcc tcgccggccg
      421 cgacccggct ggtccggatg tagtcgagca gcgacgtctt gccgtggtcg acgtgaccca
      481 tcacggtgac gacgggggcg cgcgactcgg ccggctggtt cgattcggtc tcggcgtcct
      541 cgaggaaggc ttccggatcg tcgagcttgg cggccgtcgc cgtgtggccc atttcctcga
      601 cgacgatcat cgccgtctcc tggtcgagct gctggttgat ggtcaccatc tggcccagct
      661 tcatcagctg cttgatgacc tccgaggcct tcaccgacat cttgtgcgcg aggtcggcga
      721 cgctgatcgt ctcgggaata tgcacttcct gcgcctggaa ctcggccggc gcgacgaagg
      781 tgctcggggc gccgtcgttg cggtcgccac gacggcctgc tcgcggtgcg cgccagccgg
      841 cgcgtccggc acccggcgcg ccgcgagggc ccgccttcag ggcccgcttc ttggccgcgt
      901 cgtcggccca gctcgaggag agcttctccg acttgatcga tttcttgtcg ccgggcttgg
      961 cggccgtggt cgtcgtcgcc cccggcgtgc cggccttctt gtggatcgtt cccttgatcg
     1021 cttccttggc gggctcggcg ggcttggctt cttcggcctt cttggcgacg agcaccttct
     1081 tgggcgtcga catcatggtg cggatcgccg ccgcctcgtc ttccgccgcc ttgcgccgct
     1141 tggccagatc ggcggcgcgc tgcttctcct cgttctcgat ggtgccggcc ttgacgacgc
     1201 gcagtgccgg cttgcccggc tcggcggctt tcccttcggc agccttcgac tcggcgccct
     1261 tgccttccgg ggccttcgcg tcggcgacct tctcgggcgc gggtttcccg gcggtcgctt
     1321 cacccgccgc ggcagccgct gccgcagcct cggccgccag tttggcggcc gcctcgcggg
     1381 cgcgcagctc ggccacttcg cgctcggcac gttcctgtgc ttccctgcgg cgttgctgct
     1441 cgcgcaattc ctcttcctgc acgcgcaact gctcggcctc ggcgtgcgct tcctcctcgc
     1501 ggcggcgcaa ctcggcctcg tgcgactcgg cggccgcggc ctcgtcgacc ggggcggccg
     1561 cgacttcgtc gcgcttgacg aaggtgcgct tcttgcggac ttcgacctgg atcgtgcgcg
     1621 ccttgccgct cgagtcggcc tgcttgatct cgctggtcga cttgcgtgtc agggtgatct
     1681 tcttgcgctc gcccgcaccc ggcgcggtgc cgtgcgatga gcgcagatgg tcgagcaggc
     1741 gggccttgtc ggtttcggtc acggagtcgt cggctccggc cttgccgaca cctgccgact
     1801 tgagctgctc gagcagcgcc gcggccgggc gattcagctc cgcggcgagt tgggcgacgg
     1861 tggtcgatgc catggttgta tctatccttg cgttgcggct tcttgcgtgg cggagtcggc
     1921 cggcgcgtct tgcgacgacg cggcctgcgc ttcggcagcg aaccagtgtt cgcgagcctt
     1981 catgatcagg tctttcgcca catcggcgtc gacccccgaa atctcgatca gctcgtcgac
     2041 cgcgaggtcg gcgagctcgt cgcggctgtg cacaccgccg gcgttgagct tgtcgatcag
     2101 ctcgggcgtc aggccgacga agtcgtgcag gtcctgcgag acgatgccgc cgttctcggc
     2161 ctgggccagc tccatcgtca ggagggcctg cttggcgcgg gcgcgcagct cgttgaccgt
     2221 gtcttcgtcg aagctttcga tctcgagcat ctcctgcatc ggcacgtagg ccacttcctc
     2281 caggctcgag aagccctcgg cgatcaggat gtcggccact tcggcatcga cgtcgagctt
     2341 gtcgacgaag agcttgcgaa ccgaatcgga ttcgttggcc tgcttcgacg ccgactcctc
     2401 ggccgtcatg atgttgatcc gccagccggt catctcggaa gcgaggcgga cgttctggcc
     2461 gccgcggccg atcgcgatcg ccaggttctc ctcgtcgacg acgacgtcca tcgcgtgacg
     2521 ctcctcgtcg acgacgatcg actgcacgtt ggccggcgcc agggcgccga tgacgaactg
     2581 cgccggatct tccgaccaga gcacgatgtc gacccgctcg ccggcgagct cgttggtgac
     2641 ggcgttgacg cggctgccgc gaacgccgac gcaggtgccg atcggatcga cgcgcttgtc
     2701 gtgcgactgc acggcgatct tggcgcgcga gcccggatcg cgggcgcacg acttgatctc
     2761 gagcaggccc tgctccatct cgggcacttc ctgcttgaag agctccttca tgaagcccgg
     2821 cgacgagcgc gacaggatga tctgcggccc gcgcagggtc gggtcgacct cggcgatgta
     2881 ggcccggaca cggtcgccgg tgcgcaggtt ttccttgggg atcatctccg agcggcgcag
     2941 gcgcccctcg atgcgacccg actcgacgac gatgtcgccc ttgtcgaggc gcttgacggt
     3001 accgacgaag atcttctcgc cgcgcgacag gaagtcggtg agaagctgct cgcgctcggc
     3061 gtcgcggatc ttctgcagga tcacctgctt ggccgcctgc gcgccgatcc ggccgatcga
     3121 taccgactcg atctgctcct cgatgtagtc gtcgacctcg atgtcggcga tctgctcgac
     3181 cgcctcgaag tgcaggacct cggaatcggg gagctgcagg ccggcctcgt ccggcacgac
     3241 gtgccagcgc cggaatgtct cgtactcgcc ggtctcgcgg tcgaccgaca cgcggatatc
     3301 gacctcgccg ccttgcagct tcttcgacgc tgaagcaagc gccgcctcga ctgcctggaa
     3361 aacgatctcg aggtcgacgc tcttctcgcg cgagatcgcg tccaccagca tcaacagttc
     3421 acggttcatt gaatccgacc tccatccatc tcttgccccg cgccggcctc atgagccgac
     3481 gccgcgcctt ggggggccga ccggcggccc ttgaaatcga tcaccgggac gagccgcgcc
     3541 tcgcggacct cgccgagaga aaagtcgagc accttgaccg gcccctcggc cgcgggcgcg
     3601 tcgccttcgc cggccgcagc gcggcgcttt tgcgccgaac ggctcacggc gtggcgccgc
     3661 gccgcggccg ccggcgcggt cggcgccagc accagccgcc aatcgtcgcc acgcgcctgc
     3721 aactcgcctc gaaaccgctt gcggccctgg aaggcctcct tgagcgtcag ctcgacggcc
     3781 tcgccttcga aacgggcgaa gtccgccgcc gtgcgcagcg gtcggtcgag accgggagaa
     3841 gacacctcga ggcgctcgta ggctaggccc tcgacctcga ggacgtgctg cagctgccgt
     3901 gtcaccttct cgcagtcgtc gacggtgatg gcgcgcgacc gccccgccac cgccgggtgg
     3961 tcgatcgtca cgcgcagcag tccacccgct tcgcgctcga catcgacgag ctcgtatccg
     4021 agcccgcgaa cggtcgtgtc gatggcagct tgctgggtca tgaagagtct gggcgggcga
     4081 ttcgggcaat aagtcgcggc gggaacaggc agcaaaagac gatcagccaa aaaaaatggg
     4141 ccggaagatc cgcccattga cgctatctgg gaaaacagga attgtatcct tcctcgcgat
     4201 taccggcaag caaccgccct gtcaagaggc caggaccgcg tccgaccgcg tgccagaatc
     4261 cgccgccaca ccgccctttc ggagcttctc gatgggattt ctcgcaggca agcgcctcct
     4321 cgtcaccggc ctgctctcga atcgctcgat cgcctacggc attgcccgcg cctgtcatcg
     4381 ggagggcgcc gagctggccc tgagctacca gggcgagcgc tttttgcctc gggtcaccga
     4441 attcgccgcc gagttcggct cgaagctggt cttcgactgc gacgtcgccg acgacggcca
     4501 gatcgagcgg atgttcaccg gcctcggcga ggcgtggccg acc
//
LOCUS       DASRWS010000359         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2723909, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000359 DASRWS010000000
VERSION     DASRWS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723909"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1924
                     /locus_tag="VFF72_06775"
     CDS             158..1924
                     /locus_tag="VFF72_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZV92905.1"
                     /translation="MAEERVERRLAAILCADVAGYSRLMGANEEKTLAVLKGHRRELI
                     DPLIDQHRGRTFKTTGDGMLIEFASVVDAVRCAVVIQSGMDDRNADVDEERRIRFRVG
                     INVGDIIVEGDDIFGDGVNVAARLETLAQPGHICVSATVREQIGEKLPICFADMGEHT
                     VKNIARPIHVFRIDTGVEAAAAAHQDATPAVRPSVRASIAVLPFQNMSGDPEQDYFCD
                     GMVEDIITGLSRIKSLFVIARNSTFIYKGRAVDIKQVGRELGVRYVLEGSVRKGGNRV
                     RITGQLIEVDTGTHVWAERYDRPLDDVFAVQDEIALNVVGAIEPTLRQAEIERVKYER
                     PDSLGAYDLVLRALPDIYVAMTDEATRALELLDRALALEPDYALAHAYAAWGHEIRFV
                     RGGQSPVERDAALRHAHGAIAHGPDDAIALSLAAFVVAMVAHDMVTARKAFEQALAIS
                     PSCFFALCFGAAAFGWKGDADRAIEWGERGRRLSPFDRLLYGPGHGLALGYFMRGDSA
                     KAAEGARIAIQAKPEFSLSHILLAAALAQGGHLEAAKAAGAQALALQPNFSARGLCAA
                     VGIPPALAEPLSAALRDAGLPA"
     gene            complement(1921..>2317)
                     /gene="prpE"
                     /locus_tag="VFF72_06780"
                     /gene_synonym="yahU"
     CDS             complement(1921..>2317)
                     /gene="prpE"
                     /locus_tag="VFF72_06780"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900953.1"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HZV92906.1"
                     /translation="AGHRLSTGQIEEVLASHDAVAECACVGIADEMKGQVPLGFLVLK
                     SGVSRPEGEIVSEVVQMVRDRIGPVAFFKQALVVPRLPKTRSGKILRGTMQKIADGQP
                     WKMPATIDDPAALEEVTGSLKSAGFASKS"
BASE COUNT          367 a          789 c          802 g          359 t
ORIGIN      
        1 cgaagaaaga cgcgcgcggc cgcagccgac gcccaagaca aagtatcctg ccaccaatcc
       61 gatagggact ttgacgtacg gcgaattcgg gcaaacggaa gatcgactgc cacgagcggg
      121 gctgctatcc tcgcttctcc tcgcggggag cgggaggatg gcggaggaac gggtagagcg
      181 gcggctggcg gcgatcctgt gcgccgatgt cgccggttat tcgcggctga tgggcgcgaa
      241 cgaggaaaaa accctcgccg ttctcaaggg acaccggcgt gaattgatcg atccgctgat
      301 cgaccaacac cgcggacgca ccttcaagac caccggcgac ggcatgctga tcgagttcgc
      361 ctcggtggtc gacgccgtgc gctgcgccgt cgtgatccag agcggcatgg acgaccgcaa
      421 cgccgacgtc gacgaggagc ggcgcatccg gtttcgcgtc ggcatcaatg tcggcgacat
      481 catcgtcgag ggcgacgaca tcttcggcga cggcgtgaac gtggcggcgc ggctggagac
      541 gctggcgcag ccgggccaca tctgcgtcag cgcaacggtg cgcgagcaga tcggcgagaa
      601 gctgccgatc tgctttgccg acatgggcga gcataccgtc aagaacatcg cccggccgat
      661 ccacgtcttt cgcatcgaca ccggcgtgga ggctgcggcc gcggcccatc aggacgcgac
      721 acctgccgta cggccgtcgg tacgcgcctc gatcgcggtg ctgcctttcc agaacatgag
      781 cggcgatccg gagcaggact atttctgcga cggcatggtc gaggacatca tcaccggcct
      841 gtcgcgcatc aagtcgctgt tcgtgatcgc ccgcaattcg accttcatct acaagggccg
      901 cgcggtcgac ataaagcagg tcggccgcga gctcggcgtg cgctacgtgc tggagggcag
      961 cgtgcgcaag ggcggcaatc gcgtgcgcat caccggccag ctgatcgagg tcgataccgg
     1021 cacgcatgtg tgggccgagc gctacgaccg gccgctcgac gacgtgttcg ccgtgcagga
     1081 cgaaatcgcc ttgaacgtgg tcggcgcgat cgagccgacc ctgcggcagg cggagatcga
     1141 gcgggtgaag tacgagcggc ccgacagtct cggcgcctac gatctcgtcc tgagggcgct
     1201 gcccgacatc tacgtggcga tgacggacga ggcgacgcgg gcactcgaat tgctcgaccg
     1261 cgcgctcgcg ctcgagccgg actatgcgct ggcgcacgcc tatgccgcct ggggtcacga
     1321 gatccgcttc gtccgcggcg gccagagccc cgtggagcgc gatgccgcgc tgcgccacgc
     1381 ccatggtgcg atcgcccatg gtccggacga tgccattgcg ctctcgctgg ccgccttcgt
     1441 cgtcgccatg gtcgcgcacg acatggtgac ggcccgcaag gcgttcgagc aggcgctggc
     1501 gatcagcccg tcctgcttct tcgccctgtg cttcggcgcg gcggcgttcg gctggaaggg
     1561 cgacgcggac cgcgcgatcg aatggggcga gcgcgggcgg cgcctcagcc cgttcgatcg
     1621 cctgctgtac gggccgggcc atggcctggc gctcggctat ttcatgcggg gcgacagcgc
     1681 gaaggccgcc gagggcgcgc gtatcgccat ccaggccaag cccgaattca gcctgtcgca
     1741 catcctgctg gcggcggcgc tggcccaagg aggccatctc gaggcggcga aggccgccgg
     1801 cgcgcaggcg ctggcgctgc agcccaactt cagtgcgcgc ggcctgtgcg cggcggtcgg
     1861 cattccgcct gccctcgccg aaccgctgtc ggcagcgctg cgcgacgccg gcctgcccgc
     1921 ctagcttttc gacgcgaagc cggccgactt caggctgccg gtgacttcct cgagtgccgc
     1981 cgggtcgtcg atcgtcgccg gcatcttcca cggctggccg tcggcgatct tctgcatggt
     2041 gccgcgcagg atcttgcccg agcgggtctt gggcagccgc ggcacgacca gcgcctgctt
     2101 gaagaaggcg accgggccga tgcggtcacg caccatctgc acgacctcgc tgacgatctc
     2161 gccttccggc cggctgacgc ccgacttcag caccaggaag ccgagcggca cctgcccctt
     2221 catctcgtcg gcgatgccga cgcaggcgca ttcagcgacc gcgtcgtggc tcgccagcac
     2281 ctcctcgatc tgcccggtcg acaggcgatg gccggcg
//
LOCUS       DASRWS010000360         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3030668, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000360 DASRWS010000000
VERSION     DASRWS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030668"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /gene="obgE"
                     /locus_tag="VFF72_06785"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             <1..335
                     /gene="obgE"
                     /locus_tag="VFF72_06785"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229143.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZV92907.1"
                     /translation="VDAVEQARATVRELEKYDAELAAKPRWIVLNKIDMIATEDRAQR
                     VRDFSKRLKWKGPVFPISALTREGCEPLVRAIFAHIAATVEAPAAPLDPRFDRDPSAD
                     GDSGSADR"
     gene            332..1450
                     /gene="proB"
                     /locus_tag="VFF72_06790"
     CDS             332..1450
                     /gene="proB"
                     /locus_tag="VFF72_06790"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZV92908.1"
                     /translation="MSHRRPDPRRIVVKVGSSLVTDEGRGVDPKAVGGWCRQMAALVR
                     DGRDVVMVSSGAISEGMKRLGWTSRPQAVHQLQAAAAVGQMGLAQIYESALREFGTSS
                     AQVLLTHADLADRERYLNARSTLLTLLGLRVVPVINENDTVVNDEIKFGDNDTLGALV
                     TNLLDADLLVILTDQRGLFSADPRKDPAARLVERGIAGDPDLERMAGGAGSAIGRGGM
                     LTKVLAAARAARSGASTVIASGREQDVLLRIVEGESLGTSLVAATAKVAARKQWMSNH
                     LQLRGSVTIDGGAANKIKDSGKSLLPIGVVAVEGDFHRGDVIAVRDASGSEIGRGLSN
                     YGSAEARLIARKPSSEFHALLGFVGEPELIHRDNFVLS"
     gene            complement(1499..2173)
                     /locus_tag="VFF72_06795"
     CDS             complement(1499..2173)
                     /locus_tag="VFF72_06795"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZV92909.1"
                     /translation="MLDRDGFRPNVGIVLLNPKNQVFWGKRIRTHSWQFPQGGINFGE
                     TPEQAMFRELHEEVGLTQDHVRIVARTRDWLRYEVPMHFIRRDARGHYRGQKQIWFLL
                     QLIGRDCDMNLRATDHPEFDAWRWNDYWIPLDVVIEFKRDVYQMALTELARFLPRSAQ
                     HNRYLRAGMRPHHRDEQADRFQKREGAEPTSQFDSDDAHEEPTAETGLSAATRTSPPR
                     SEGSPH"
     gene            2310..>3717
                     /locus_tag="VFF72_06800"
     CDS             2310..>3717
                     /locus_tag="VFF72_06800"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZV92910.1"
                     /translation="MKASKFFISTLKEAPADAEVASHRLMLRAGFIKRLSAGIYTYMP
                     MGLRVIRKIENIVREEMNAAGAVELLMPVVQPAELWQESGRFAKYGPELMRVKDRHER
                     DFVIQPTSEEVITDIARQELRSYRALPRNFYHIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRDLDAARASYDAMYAAYVRIFERMGLAFRAVAADNGSIGGIRSHEFQVIADTGE
                     DALVFSRESDYAANIELAEALAPIAPRAEPKAPLAKASTPGTTTCEAVAKLLGLPLDR
                     TVKSIVLATDAKAEEGGSNRPGDVWLLLLRGDHALNEIKTGKVAGLDAGFRFATASEI
                     EAHFGSPPGYLGPIGTRLPVRLVVDRSVATMSDFVCGANERDFHFTGVNWGRDLPEPE
                     AVADIRDVVEGDPSPDGAGSLSIQRGIEVGHVFLLGSYYSKCMQATFLDEAGKPQLLQ
                     MGCYGIGITRIVGAAIEQN"
BASE COUNT          650 a         1197 c         1224 g          646 t
ORIGIN      
        1 gcgtcgatgc ggtcgaacag gctcgcgcga ccgttcgcga gctcgaaaag tatgacgccg
       61 agttggccgc gaagccgcgc tggatcgtcc tcaacaagat cgacatgatc gcgacggagg
      121 accgcgcaca gcgggtgcgc gacttctcga agcggctgaa atggaagggg cccgtcttcc
      181 ccatctcggc acttactagg gagggctgcg aacccttggt gcgcgccatc ttcgcgcaca
      241 tcgccgccac cgtcgaggca cctgctgccc cgctcgatcc acgattcgat cgagaccctt
      301 cggccgacgg ggattcgggt tcggccgatc gatgagccat cgccgtcccg atcctcgacg
      361 gatcgtcgtc aaggtgggat cgagcctcgt caccgacgaa ggccgcggtg tcgaccccaa
      421 ggcggtcggc gggtggtgtc gacagatggc ggcgctcgtg cgcgacgggc gagatgtcgt
      481 catggtgtcg agtggcgcga tatccgaggg catgaagcgg ctcggctgga ccagccgtcc
      541 ccaggccgtt catcaactgc aggcagcggc ggcggtcggc cagatgggat tggcgcagat
      601 ctacgagtcg gcacttcgcg aattcggtac gtcgagcgca caggtattgc tgacgcatgc
      661 cgatctggcg gatcgcgagc gctatctcaa tgcccgctcg acgctcctca ccctgctcgg
      721 gctgcgggtc gtgcctgtca tcaacgagaa cgacaccgtc gtcaacgacg agatcaagtt
      781 cggtgacaac gacacgctcg gcgccctggt gaccaacctt ctcgacgccg atctgctcgt
      841 gatcctgacc gatcaacgcg gcctcttctc ggccgatccg cgcaaggacc ccgctgcccg
      901 cctcgtcgag cgcggtatcg ccggcgatcc ggacctcgag cggatggctg gcggcgcggg
      961 cagtgcgatc ggtcgcggcg gcatgctgac caaggtgctg gcagccgcgc gggcggcgcg
     1021 aagcggggcc tccacggtca tcgcctcggg ccgtgaacag gatgtgcttc ttcgcatcgt
     1081 cgaaggggaa tccctgggca cgtctctcgt cgccgcgacc gcgaaagtgg cggctcgaaa
     1141 gcagtggatg agcaaccacc tgcaactgcg cgggtcggtg acgatcgacg gcggggccgc
     1201 caacaagatc aaggacagtg gcaagagcct gctgccgatc ggcgtcgtcg ccgtcgaggg
     1261 cgatttccat cgcggtgatg tcatcgccgt gcgagacgca agcggcagcg agatcggtcg
     1321 cggcctgagc aactacggta gcgccgaggc gcggctcatc gcgcgaaagc cgtcgagcga
     1381 attccacgcg ttgctcgggt tcgtcggcga gcccgaactg atccatcgcg acaatttcgt
     1441 cctgtcgtga cgccggtccc ggtgtcgtcg ccgcgccgcc agaaacacca tcgacctctc
     1501 agtgcggcga cccttcgcta cgcggcggtg aagtccgcgt ggccgccgac aggccggtct
     1561 cggccgtggg ctcctcgtgg gcatcatcgg agtcgaactg gctcgtcggc tctgcgcctt
     1621 ctcgcttctg gaatcggtcg gcctgctcgt cacgatgatg cggacgcatg cccgcccgca
     1681 gatagcggtt gtgctgcgcg ctgcggggaa gaaagcgagc cagttcggtc agcgccatct
     1741 gatagacatc gcgcttgaac tcgatgacga catcgagcgg gatccagtaa tcgttccagc
     1801 gccaggcatc gaactccgga tgatcggtgg cgcgcaggtt catgtcgcag tcgcggccga
     1861 tgagttgcag caggaaccag atctgctttt gtccgcggta gtgcccccgt gcatcgcggc
     1921 ggatgaaatg catcgggact tcatagcgca accagtcgcg cgttcgcgcg acgatcctga
     1981 catgatcctg cgtcagcccc acttcctcgt ggagttcgcg aaacatcgcc tgctcgggcg
     2041 tctcgccgaa attgatgccc ccctgaggga actgccagga gtgggttctg atccgtttgc
     2101 cccagaacac ctggttcttc gggttgagca ggacgatgcc gacgttgggt ctgaacccat
     2161 cacggtcgag cataatcaac ctcgattctt gcgttgaccg attattgcac cggcatgacg
     2221 cgagtcaaac ctcgcgttcc cgggtatgcc gaaaggccag tcgccgggtc gcgcaccgcg
     2281 ccgccggggc gcgcaacgat cacgcgacga tgaaagccag taagttcttc atttcaacgc
     2341 tgaaggaagc cccggccgat gccgaggtcg ccagccaccg gctgatgctg cgcgccggct
     2401 tcatcaagcg tctgtcggcg ggcatctaca cctatatgcc gatgggtctt cgcgtcatcc
     2461 gcaagatcga aaacatcgtg cgcgaagaga tgaacgctgc cggggcggtc gagctcctca
     2521 tgccggtggt ccagccggcg gaactgtggc aggagagcgg ccgctttgcg aagtacgggc
     2581 ccgagctgat gcgcgtcaag gaccggcacg agagggactt cgtcatccag ccgacgagcg
     2641 aggaggtcat caccgacatc gcccgccagg aactgcgcag ctatcgcgcg ctgccccgaa
     2701 acttctatca catccagacg aaattccgcg acgagcgtcg tccgcgtttc ggtgtgatgc
     2761 gcgggcggga gttcacgatg aaggacgcct attccttcga ccgcgatctg gatgcagccc
     2821 gagcgagcta cgacgcgatg tatgcagcgt acgtacgtat cttcgagcgc atggggctcg
     2881 cgtttcgtgc tgtcgccgcc gacaacggga gcatcggcgg cattcgttcg cacgagttcc
     2941 aggtgatcgc cgataccggc gaggacgccc tcgtcttcag ccgcgaatcg gactacgccg
     3001 ccaacatcga gctggctgaa gcgctggcac caatcgcgcc ccgggccgaa ccgaaggcac
     3061 ctctggcgaa ggcgtcgacg ccgggcacga ccacgtgcga ggccgttgcg aagctgctcg
     3121 gcctgccgtt ggaccggacc gtgaaatcga tcgttctcgc aaccgatgcc aaggcggaag
     3181 agggaggttc gaaccggccg ggtgatgtat ggctccttct gctgcgcggc gaccacgcgc
     3241 tcaacgagat caagaccggc aaggtcgccg gcctcgacgc ggggttccgg ttcgcgacgg
     3301 cgtccgagat cgaggcgcat ttcggcagcc cgccgggata cctcgggccc atcggcacga
     3361 ggcttcccgt ccggctcgtc gtcgaccgca gcgttgccac gatgagcgac ttcgtgtgcg
     3421 gcgccaacga gcgcgatttt catttcacgg gggtcaactg gggccgcgat ctgcccgagc
     3481 ccgaggcggt cgccgatatc cgcgatgtcg tcgagggcga tccttcgccc gacggcgcgg
     3541 gttcgctttc gatccagcgt ggcatcgagg tcggccacgt gttcctgctc ggctcgtact
     3601 acagcaagtg catgcaggcc acctttctcg acgaggccgg caagccgcaa ttgctgcaga
     3661 tgggctgcta cggcatcggc atcactcgca tcgtcggggc cgccatcgag cagaacc
//
LOCUS       DASRWS010000361         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1011501, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000361 DASRWS010000000
VERSION     DASRWS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011501"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFF72_06805"
     CDS             <1..893
                     /locus_tag="VFF72_06805"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZV92911.1"
                     /translation="FREKRDVQFSTLIEFAIKYGKPVRIGVNWGSLDQALLTRLMDEN
                     ATAAAPLTAAEVVREAIVQSALLSAAQAEEIGLPRSKIILSAKVSQVQDLIAVYTMLA
                     QRSDHALHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRVSLTPEPGGDRTQEVKV
                     AQEILQTMGFRVFVPLVAACPGCGRTTSTTFQELAGDIQKFIRDSMPVWKTRYPGVEA
                     LNVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFIDGKKAATLRGPTLAADFQKM
                     VIDYIERRYGTSGAGQTAAE"
     gene            1143..>2144
                     /locus_tag="VFF72_06810"
     CDS             1143..>2144
                     /locus_tag="VFF72_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92912.1"
                     /translation="MSRLRRRVAFHSLIWVVICLVALAVVAIGATAWGLRADQIAAGY
                     RETDRTASMLADQTTRSITTLDAGLKDMRAHIAGLGIDTPDDFSRRAGTSEMFRYLTQ
                     RLALLPQATAITLTGSDGRVVNTTRQWPRPDANFSDRDFFKYLSRVDDNQIYVNLPVK
                     SRLGGTLTVYFSRRVNARNGAFAGVVAIAVEIEYFANIYESVSAVSGQQFLLLRRDGT
                     TIWRYPEPVHRLGEKLPADSAFFAAVRNGGGSYRSLGRLHRGVRLVSVRPLRDYPLVV
                     NVALLENSVLASWNRRMLAIGAGTLLVVCCAGVLLRIWAKQLGRLIASEAQLARKSQE
                     "
BASE COUNT          352 a          744 c          687 g          361 t
ORIGIN      
        1 gcttccgcga aaagcgcgac gtgcagttct caacgctcat cgagttcgcc atcaagtacg
       61 gcaagccggt ccgcatcggc gtcaactggg gctcgctcga ccaggcgctg ctcacccgac
      121 tgatggacga aaacgcgacg gccgccgcgc cgctcacggc ggccgaggtc gtgcgcgagg
      181 cgatcgtgca atcggcgctt ctgtcggcgg cgcaggccga ggagatcggc ctgccgcgtt
      241 cgaagatcat cctgtcggcg aaggtcagcc aggtgcagga tctgatcgcg gtctacacca
      301 tgctcgccca gcgatccgat cacgcgctgc atctcggcct caccgaggcc ggcatgggct
      361 cgaagggcat cgtcgcgtcg tccgccgcgc tcggcatcct gctgcagcag ggcatcggcg
      421 acaccatccg cgtttcgctc acgcccgagc cgggcggcga ccgtacgcaa gaggtcaagg
      481 tcgcgcagga gatcctgcag accatgggct tccgggtgtt cgtgccgctc gtcgcggcct
      541 gcccgggctg cggccgcacg acctcgacga ccttccagga gctcgccggc gacatccaga
      601 agttcatccg cgactcgatg ccggtgtgga agacgcgtta cccgggcgtc gaggcgctca
      661 acgtcgcggt gatgggctgc atcgtgaacg gcccaggcga gagcaagcac gccgacatcg
      721 gcatctcgct ccccggcacc ggcgagacgc cgaccgcccc ggtcttcatc gacggcaaga
      781 aggccgcgac gctccgcggc ccgacgcttg ccgccgattt ccagaagatg gttatcgact
      841 acatcgagcg ccgctacggc acgagcggcg cggggcaaac ggcagcggaa taattcctcc
      901 tggagcagcc cggattgagc tgcgctcaat ccgggccatg accgcccata cttcgacgcg
      961 gtctatcgtt gataatgctg gcgcgagcgc ccaaaacgac ccctcagctc gccccgattt
     1021 gatcgcctgc tactctgccg tgtaagcagc cggtatcgca gccgaaaaag ccaaatttat
     1081 taataagtcg cggctaggct ggcttcaatt tgattcaagt tggacctgcc attggacctg
     1141 ccatgtcccg cctccggcgc cgcgtcgcat ttcacagcct gatctgggtc gtcatctgcc
     1201 tggttgcgct cgccgtggtg gcgatcgggg cgaccgcctg gggcctgcgc gccgaccaga
     1261 tcgcggccgg ttatcgcgag accgaccgca ccgcgagcat gctggcggac cagaccaccc
     1321 gctcgatcac gaccctcgac gccggcctga aggacatgcg ggcccatatc gccggcctcg
     1381 ggatcgacac acccgacgac ttttcgcgcc gcgccggcac gtcggagatg ttccggtatc
     1441 tcacccagcg gctggccctg ctgccgcagg cgactgcgat cacgctcacg gggagcgacg
     1501 gccgggtcgt caacacgaca cggcaatggc cgcggcccga cgcgaatttc tccgaccgcg
     1561 actttttcaa gtacctgagc cgggtcgacg acaaccagat ctacgtcaac ttgccggtga
     1621 agagccggct gggcggaacg ctcaccgtgt acttcagccg gcgtgtcaac gcccgcaatg
     1681 gcgcgttcgc gggcgtcgtc gcgatcgcgg tggagatcga gtacttcgcc aacatctacg
     1741 agtccgtctc tgccgtgagc ggccagcagt tcctgctgct gcgtcgcgac ggcaccacga
     1801 tctggcgcta ccccgaacca gtccaccgac tgggcgagaa gctgccggcc gactcggcct
     1861 tcttcgccgc cgtccgcaac ggcggcggca gctatcgttc actcgggcgt ctccatcgcg
     1921 gcgtccggct ggtctcggtc cgtccgctgc gggactatcc gctcgtcgtc aatgtcgcgc
     1981 tcttggagaa ttcggtgctg gcgagctgga accggcgcat gctcgcgatc ggtgccggca
     2041 cgcttctggt cgtctgctgc gcgggcgtgt tgctgcggat ctgggcaaag cagctcggcc
     2101 ggctgatcgc ctccgaggcg cagctcgccc gcaagtcgca ggag
//
LOCUS       DASRWS010000362         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2431684, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000362 DASRWS010000000
VERSION     DASRWS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2431684"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1690)
                     /locus_tag="VFF72_06815"
     CDS             complement(377..1690)
                     /locus_tag="VFF72_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZV92913.1"
                     /translation="MSPDSTSSSNEPPAVRGALAGIRVLDLSRVLAGPWCTQVLADLG
                     ADVFKIERPPGEGHPGGDDTRGWGPPFLRDRNGAETAEAAYFLGTNRNKRSITVDIAT
                     PAGADLVRDLATRCDVLVENFKVGDMARRGLDSSSLLALNPRLVYCSITGYGQTGPYR
                     DRAGYDYAIQGLGGLMSVTGPSRKEIDDDAPGGGPQKVGVAVADLFTGMYAATAILAA
                     LRHRDATGEGQAIDMALLDTQVAMLANLGANYLVTGVAPQRAGNAHQNIVPYQVFEVA
                     DGHLILAVGNDGQFGRFCAVAGRPELATDARFAVNSARVRHRDVLVPILRDVFRTRTR
                     ADWLAAFEAATVPCGPINDLADVFADPQVQARSMTVSLDHPSAGSVRLVASPMKLSRT
                     PVAYRSAPPLLGAHTDDALLELGLSADRIGELHAAGALGPGTTNP"
     gene            1790..2275
                     /locus_tag="VFF72_06820"
     CDS             1790..2275
                     /locus_tag="VFF72_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92914.1"
                     /translation="MAACSPALDWRQLPSSETGVTVLFPCRPERLVRDVPLDHVALPM
                     RLWSCTASGATFSLALVDVATAARVGPTLSSWRVHASANLGLAPVALPYVVPGATPND
                     GSEAFAIATRPTGGPRIVGRTAFFVHGLRLYEATVLGAAPDTEAMEAFFGSIRVATPR
                     P"
     gene            2322..>2510
                     /locus_tag="VFF72_06825"
     CDS             2322..>2510
                     /locus_tag="VFF72_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HZV92915.1"
                     /translation="MPGLLIVAHAPLASSLKAVAQHAYPECAARLEALDVAADAAIDE
                     VEAQARTLLERVGTPDVLI"
BASE COUNT          375 a          826 c          886 g          423 t
ORIGIN      
        1 tcgccgtgag gatgtggatt cgttcgagcg ccgccgtcaa gggcgcagcg atctccgagc
       61 cgacctggga cacgagttcg tgccaccgtt ggtcctctgc cgtgtcgtcg ggggccgggg
      121 catgggcttg ccccgcagcg gggcggttgg cttctgacat gtcgacgggg tcgctgatct
      181 cgggaaaatt cgggcgggcg cggtgccggg gcgatctcgc aggaggccac cggggcgacg
      241 gccggaccac gaacgacgcc gcgtcgggcg caatcgcgtc agcgcgaagt gtcggcgcgc
      301 cgaccctgcg gcgccagagc cggtgccgac cgtgccgacc agcggtcggg caagccgggg
      361 aaatgtgaca acttgttcac ggattcgtcg tgccggggcc gagcgctcct gcggcgtgaa
      421 gttcgccgat gcgatcggcc gacaggccga gctcgagcag cgcgtcatcg gtgtgtgcgc
      481 caagcagagg cggtgctgag cggtatgcga caggagtacg ggataacttc atcggactcg
      541 caacgaggcg aaccgagccc gccgaaggat gatcgagtga aacggtcatc gagcgcgcct
      601 gcacctgcgg atcggcgaaa acgtcggcca gatcgttgat ggggccgcag ggcaccgtgg
      661 ccgcttcgaa cgccgcgagc cagtccgcgc gggttcgtgt gcgaaacacg tctcgaagaa
      721 tgggcaccag cacgtcgcgg tgcctgactc tcgccgagtt gacggcaaag cgtgcatcgg
      781 tcgccaactc tggccgccct gcgacggcac agaagcgtcc gaattgcccg tcgttgccga
      841 cggcgaggat gaggtgaccg tcagcaactt cgaagacctg gtacggcacg atgttctgat
      901 gtgcgttgcc tgctcgctgt ggcgccacgc cggtgacgag gtagttggcg ccgaggttgg
      961 cgagcatggc gacctgggtg tcgagcagcg ccatgtcgat ggcctggcct tctccggtgg
     1021 cgtcccggtg gcgcagggcc gccaggatcg ccgtcgccgc atacatgccg gtgaacaggt
     1081 cggcgacggc gacgcccacc ttctgcggtc cgccgcccgg cgcgtcgtcg tcgatctcct
     1141 ttcgcgacgg acccgtgacg ctcatcaggc cgccgagccc ctggatggcg tagtcgtagc
     1201 cggcacggtc gcgatagggt ccggtctggc catagccggt gatcgagcaa tagaccagcc
     1261 gcgggttcag cgcaagcagc gatgacgagt cgagaccgcg acgagccatg tcgccgacct
     1321 tgaagttctc gaccagcaca tcgcagcggg tcgcaaggtc acggaccaga tcggcgcccg
     1381 cgggtgtcgc gatgtcgacc gtgatggagc gcttgttgcg attggtgccg aggaaatagg
     1441 cggcctcggc cgtctccgca ccgttcctgt cgcggaggaa aggagggccc cagccccggg
     1501 tatcgtcgcc tcccggatga ccttcgccag gcggccgctc gatcttgaaa acgtcggcgc
     1561 cgaggtcggc cagcacctgc gtgcaccagg ggccggcgag gacgcgcgac aggtcgagga
     1621 cgcggatgcc cgccaacgca ccgcggacgg cgggtggttc gttggatgag gatgtggagt
     1681 cgggactcat cgagcgattg tcggcgcagg ttcccctcgc acagcgcgga aaatcgaggc
     1741 gatgagagtt cctgggggac catcttgcgt agccggattc ctgctgttcg tggccgcctg
     1801 cagccctgcg ctcgactggc gccagctgcc ctcctccgag accggcgtga cggtgctctt
     1861 cccctgccgg ccggagcgcc tcgttcgcga cgtgccactc gatcacgtcg cgttgccgat
     1921 gcgtctttgg tcatgcaccg cctccggggc gaccttctcg cttgctctcg tcgacgtcgc
     1981 gacggcggcc agggtcggtc cgacgctttc ctcgtggcgg gttcacgctt cggccaatct
     2041 cggcttggcg cccgtcgcgc ttccttacgt cgtccccggg gcgacgccca atgacgggag
     2101 cgaagccttc gccatcgcca ccaggccgac cggcggcccg cgtatcgtcg gccgcaccgc
     2161 attcttcgtc catggccttc gtctctacga ggcgaccgtg ttgggcgcgg cccctgacac
     2221 cgaggcgatg gaggcgtttt tcggctcgat ccgtgtcgcc acaccgaggc cgtgatgcct
     2281 gcgggcgccc tcggagtgcc aactgcccat aatgcgccgc catgcccgga cttctgatcg
     2341 tcgcccatgc gccactggcg tcgtcgttga aggcagtcgc ccaacacgct tatccggagt
     2401 gcgccgcccg gctggaagcg ctcgatgtcg ctgccgatgc cgcgatcgat gaggtcgagg
     2461 cgcaggctcg tacgctcctc gagcgggtgg gcacccccga cgtcctgatt
//
LOCUS       DASRWS010000363         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1012241, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000363 DASRWS010000000
VERSION     DASRWS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012241"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VFF72_06830"
     CDS             complement(<1..162)
                     /locus_tag="VFF72_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZV92916.1"
                     /translation="MTDPLLLVPDFVLIGLGFLVCRLTPLGRPVWDGAERLVYYLLFP
                     VLLFDSIRKS"
     gene            278..1174
                     /gene="xerD"
                     /locus_tag="VFF72_06835"
                     /pseudo
     CDS             278..1174
                     /gene="xerD"
                     /locus_tag="VFF72_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
     gene            complement(1235..2215)
                     /locus_tag="VFF72_06840"
     CDS             complement(1235..2215)
                     /locus_tag="VFF72_06840"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HZV92917.1"
                     /translation="MSDTSGGVRLPTYDDVCAAATRIAGVAWHTPVLTSRTADEALGA
                     SLFFKCENFQRMGAFKFRGAYNALASFDAKQKKAGVVAFSSGNHAQAVALAARLLGMP
                     AIIVMPHDAPAAKMAATRGYGGEIVLYDRYTEDREAIGRRLASERGLTLIPPFDHADV
                     IAGQGTAAKELIEAAGPLDALFVCLGGGGLLSGSALSARALAPGCKVYGVEPEAGNDA
                     QQSFRAGTIVRIDTPVTIADGAQTQSLSPMTFGIIRRDVDDIFTASDDELVDAMRFFA
                     ERMKMIVEPTGCLSFAAARRMKEELRGARVGVVVSGGNVDLARFASLIAG"
     gene            complement(2259..3785)
                     /locus_tag="VFF72_06845"
     CDS             complement(2259..3785)
                     /locus_tag="VFF72_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZV92918.1"
                     /translation="MTTILRHADLVDSIAGALQYISYYHPADYIAHLARAYEIEQGAA
                     ARDAIAQILTNSRMCAEGHRPICQDTGIVNVFMKIGMAVRFEGFPAGIEEAVNEGVRK
                     GYLDPDNTLRASIVADPHFERKNTRDNTPAVVHVTLVPGSTVDVIVAAKGGGSENKSK
                     FVMMNPSDSLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAERAMLLAKQSLMEDIDMAE
                     LKARGPRDRTEALRIELCDKVNALGIGAQGLGGLTTVLDVKIAMAPTHAASKPVAMIP
                     NCAATRHAHFVLDGSGPAYLDPPSLDLWPDVHWAPDHERSKTVDLDRLTREEVAAWKP
                     GDRLLLRGRMLTGRDAAHKRIQDMLARNEALPVDFAGRVIYYVGPVDPVRDEAVGPAG
                     PTTSTRMDKFTEMMLARTGLFAMIGKAERGPVAIEAIRKHRSAYLMAVGGAAYLVSKA
                     IRSARVVAFADLGMEAIYEFEVKDMPVTVAVDALGTSVHETGPAEWKSKIGGIPVAVA
                     "
     gene            3868..>4451
                     /locus_tag="VFF72_06850"
     CDS             3868..>4451
                     /locus_tag="VFF72_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV92919.1"
                     /translation="MSEEPRVYEPPQSIVGNAWVSGMDAYRELCQEAERDHEAFWSRL
                     ARELISWHTPFTRVLDADSAPFFKWFEDGTLNASYNCLDRHVEAGRGDRVALIFEADD
                     GSVARVTYKDLLERTCRLANAMKARGIVKGDRVVIYMPMSVEGVVAMQACARIGATHS
                     VVFGGFSAQSLRDRIADTGAVMVITADEQMRGGKA"
BASE COUNT          662 a         1499 c         1601 g          689 t
ORIGIN      
        1 gctcttgcga atcgagtcga agagcagcac cggaaagagc aggtagtaga cgaggcgttc
       61 ggcgccgtcc cagaccgggc gaccgagcgg cgtgagccga cagacgagga agccgagccc
      121 gatcaggacg aaatcgggca cgagcagcaa cgggtcggtc atgtcgacga gtgtgccagc
      181 gcgtccgtgg cgcgcgtcgc cacggaatcg tcggcacctg tccctcgcgc gtggcgcgat
      241 cgacggctcg ccacggcagc ccctagactc ggccgcaatg gaacgtccaa gcagcgccga
      301 ggcgatcgac cgattcgtcg atgccctctg gatcgaggac ggcctggcgg cgcagtcgct
      361 ggccgcctat cggcgcgacc tggcggcgct cgcggcgtgg ctcggccagc gcgaccgccg
      421 gctcgtcgac gctggcgaaa gcgacctgcg cgagtacgcc gtggcgcgcc atggcgccag
      481 cgccgcgtcg agcgtcaacc ggcggctggc ggtgttccgg cgcttctatc gatgggcgct
      541 gcgcgagcgg ctgaccgacg ccgatccgac gctgcgcctg gccgcgtcgc gccagccgct
      601 gcgcgtgccg aagtcgctca gctaagcaca ggtcgaggcc ttgctcgacg ccggtgaggt
      661 cgactcgccg atcggcctgc gcgaccggac catgctcgag ctcatgtatg cgagcggcct
      721 gcgcgtgagc gagctggtcg gcctggcgag cgtgagcgtc agcctcgtcg aaggggcgct
      781 gcgtgtcacc ggcaaggccg gcaaggagcg gctggtgccg ttcggcgagg aggcgcatgc
      841 gtggatcgag cgatacctcg gcgaaggccg gccggcgatc ctcgccggtc gctccagcga
      901 cgccctcttc gtcaccggcc gcggcgggcc gatgacgcga cagatgttct ggacgatcgt
      961 caagggacga gcgcggcggg ccggcatcgc ggcgccgatc tcgccgcaca cgctgcgcca
     1021 tgcgttcgcg acgcatctgc tcaaccacgg cgccgacctg cgcgccgtgc agatgctgct
     1081 cggccacgcc gacatctcga caacgaccat ctacacgcac gtcgcccgcg agcgcctgaa
     1141 ggcgctgcac gcgcagcacc atccgcgagg ttgagctctc gcgcgcctga agatggattt
     1201 gcctcgtcgg ccgcgcggcc ggcagggcgg cgtctcagcc ggcgatcagg ctcgcgaatc
     1261 gcgccaggtc gacgttgccg ccgctgacga cgacgccgac ccgcgcgcct cgcagctctt
     1321 ccttcatccg ccgcgccgcg gcgaacgaca ggcagccggt cggctcgacg atcatcttca
     1381 tgcgctcggc gaagaagcgc atcgcgtcga cgagctcgtc gtcgctggcg gtgaagatgt
     1441 cgtcgacgtc gcggcggatg atgccgaatg tcatcgggct gagcgactgc gtctgggcgc
     1501 cgtcggcgat cgtcaccggc gtgtcgatgc ggacgatcgt gccggcgcga aacgactgct
     1561 gcgcgtcgtt gcccgcttcc ggctcgacgc cgtagacctt gcagccgggc gcgagggccc
     1621 gcgccgagag cgcggagccc gagagcaggc cgccgccgcc gaggcagacg aagagggcgt
     1681 cgagcgggcc ggcggcctcg atgagctcct tcgcggcggt gccctgcccg gcgatcacgt
     1741 cggcgtgatc gaacggcggg atcagcgtca ggccgcgttc cgacgcgagg cgacggccga
     1801 tcgcctcgcg gtcttcggtg taccggtcgt agagaacgat ctcgccgccg tagccgcgcg
     1861 tcgccgccat cttggcggcc ggcgcgtcgt gcggcatgac gatgatcgcc ggcatgccga
     1921 gcagccgtgc cgccagcgcg actgcctgcg cgtgattgcc cgacgagaag gcgacgacgc
     1981 cggccttctt ctgcttggca tcgaacgacg ccagcgcgtt gtaggcgccg cgaaacttga
     2041 acgcgcccat gcgctggaag ttctcgcact tgaagaagag cgaggcgccg agggcttcgt
     2101 cggcggtgcg cgaggtgagc accggcgtgt gccaggcgac gccggcaatg cgcgtcgccg
     2161 cggcgcagac gtcgtcgtag gtgggaaggc gcacgccgcc ggatgtgtcg ctcatgtggg
     2221 tctgcacgcg cagatcgcct ggccgtcgct ggcgccgctc acgccacggc gacggggatg
     2281 ccgccgattt tcgacttcca ctcggccggc cctgtctcgt gcaccgacgt gcccagcgcg
     2341 tcgacggcga cggtgacggg catgtccttg acctcgaact cgtagatcgc ctccatgccg
     2401 aggtcggcga aggcgacgac ccgggcgctg cggatcgcct tcgagacgag ataggccgcg
     2461 ccgccgacgg ccatgagata ggcgctccgg tgcttcctga tggcctcgat cgcgaccggc
     2521 ccgcgctcgg ccttgccgat catcgcgaac aggccggtgc gggccagcat catctcggtg
     2581 aacttgtcca tccgcgtcga ggtcgtcggg ccggcgggcc cgaccgcctc gtcgcgcacc
     2641 ggatcgaccg gaccgacgta gtagatgacc ctgccggcga agtcgacggg cagggcttcg
     2701 tttcgggcga gcatgtcctg gatccgcttg tgcgcggcat cgcggccggt gagcatgcgt
     2761 ccgcgaagca agagcctgtc gccgggcttc cacgccgcca cctcttccct ggtcaggcgg
     2821 tcgaggtcga ccgtcttcga gcgctcgtgg tcgggcgccc agtggacgtc gggccagaga
     2881 tcgaggctcg gcgggtcgag gtacgcgggc cccgagccgt cgaggacgaa atgcgcgtgc
     2941 cgggtggcgg cgcagttggg gatcatcgcc accggcttgc tggcggcgtg cgtcggcgcc
     3001 atcgcgatct tgacgtcgag cacggtggtg aggccgccca gcccctgggc gccgatgccg
     3061 agggcgttga ccttgtcgca gagctcgatc cgcagcgcct cggtgcggtc gcgcggaccg
     3121 cgggccttca gctcggccat gtcgatgtct tccatcaggc tttgcttggc gagaagcatc
     3181 gcgcgttcgg ccgtgccgcc gatgccgatg ccgagcatgc cgggagggca ccagcccgcg
     3241 cccatcgtcg gcaccgtctt cagcacccag tcgacgaggc tgtcgctcgg attcatcatc
     3301 acgaacttcg acttgttctc gctgccaccg cccttggcgg cgacgatcac gtcgacggtc
     3361 gagccgggaa ccagcgtcac gtggacgacg gccggcgtgt tgtcgcgcgt gttctttcgc
     3421 tcgaagtgcg ggtcggcgac gatcgatgcg cgcaacgtgt tgtcggggtc gaggtagccc
     3481 ttcctcacgc cctcgttcac cgcctcctcg atgccggccg gaaagccctc gaagcgcacc
     3541 gccatgccga tcttcatgaa gacgttgacg atgccggtgt cctggcagat cggccggtgg
     3601 ccctcggcgc acatccgcga gttggtgagg atctgcgcga tcgcgtcccg tgccgccgcg
     3661 ccttgctcga tctcgtaggc gcgcgcgaga tgggcgatgt agtcggcggg gtggtagtag
     3721 ctgatgtact ggagtgcgcc ggcaatgctg tcgacgagat cggcgtggcg aagaatcgtg
     3781 gtcatggcgc ggtgagtgca gcccggaaat tgtagtggtc gcacatgagg taaaacggag
     3841 gcgaatcatt ccgaaggaga catcgccatg agtgaagagc cgcgcgtcta cgagccgccg
     3901 caaagcatcg tcgggaacgc ctgggtatcg ggcatggatg cctatcgaga gctgtgccag
     3961 gaagccgagc gcgatcacga ggcgttctgg tcccgcctgg cgcgcgagct gatcagctgg
     4021 cacaccccgt tcacccgggt gctcgacgcc gacagcgcgc ccttcttcaa gtggttcgag
     4081 gacggcaccc tcaacgcctc gtacaactgc ctcgaccggc acgtcgaggc gggccgcggc
     4141 gatcgggtcg cgctgatctt cgaagccgac gacggttcgg tggcgcgcgt cacctacaag
     4201 gacctgctcg agcggacctg ccggctcgcc aacgcgatga aggcgcgcgg catcgtcaag
     4261 ggcgatcgcg tcgtcatcta catgccgatg tcggtcgagg gcgtggtcgc catgcaggcc
     4321 tgcgcgcgga tcggcgcgac ccattcggtg gtcttcggcg gcttctcggc gcagagcctg
     4381 cgcgaccgca ttgccgacac cggcgcggtg atggtgatca ccgccgacga gcagatgcgc
     4441 ggcggcaagg c
//
LOCUS       DASRWS010000364         7399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1012592, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000364 DASRWS010000000
VERSION     DASRWS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012592"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFF72_06855"
     CDS             <1..845
                     /locus_tag="VFF72_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV92920.1"
                     /translation="TTDILARLIAQKLGPALGTSIIIENKGGAGGSVGSEVASRAAPD
                     GYTIVGGTISSHAINVSLYPRIGYDPVKSFEPIALIGTNPTVLVVNQASPYKTLADVI
                     AAAKAGKHLTAASAGSGTSQHLSLELLKAKAGIDITHIPYKGSGPAIQDVMAGQVDMM
                     FDTTVVAGPHIESGRLRALAVTSARRLPSMPNVPTVAETVPGYEVISWQAIFAPAGTP
                     RSIVERLHDAIVKILAEPDIQARMDKLGMQGAHMTTAEVLAFQKAEVAKWAAVIKAAH
                     IKVE"
     gene            complement(859..1542)
                     /locus_tag="VFF72_06860"
     CDS             complement(859..1542)
                     /locus_tag="VFF72_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZV92921.1"
                     /translation="MSDGPNIARVAALIGDPVRAETLSALLAGRALTPTELAGLAGVT
                     KQTISGHLAQLLDAGLLAVEQQGRHRYFRLADRDVADLLESLMGVAFRTGAVRLVTGP
                     REPALRKARVCYDHLAGELGVLLYDGLLRRRYLAGGEDGLRLTRAGRSWLDAFGVDAG
                     AVLAQRRPACRACLDWGERRHHLGGAVGAALLARLFDLRWGRRAAGSRVVAFSPRGEA
                     WLRELAAGG"
     gene            1922..2095
                     /locus_tag="VFF72_06865"
     CDS             1922..2095
                     /locus_tag="VFF72_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92922.1"
                     /translation="MIDTIIKVTRAPDWNVGRPFSIGQETVALIPIASHDVEPRHSGF
                     VGLTNDDEFQMTA"
     gene            complement(2287..2362)
                     /locus_tag="VFF72_06870"
     tRNA            complement(2287..2362)
                     /locus_tag="VFF72_06870"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2327..2329),aa:Phe,seq:gaa)
     gene            complement(2406..3092)
                     /locus_tag="VFF72_06875"
     CDS             complement(2406..3092)
                     /locus_tag="VFF72_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92923.1"
                     /translation="MATILVVDDELGIRALLSEILADEGHTVELAEDAAQARACRERF
                     RPDLVLLDIWMPDVDGITLLKEWGASSLLTMPVIMMSGHGTIDTAVEATKFGAIAFLE
                     KPITLQKLLRAVEQGLAKPASRAAAVAAPAGASEAGLTVEAAMTGGAMPPSMPVMLGP
                     QPEQSFDLDRPLREARDAFEKSYFEFHLAQENGSMTRVAEKTGLERTHLYRKLKQLGV
                     DLSRNKRSPV"
     gene            complement(3100..5439)
                     /locus_tag="VFF72_06880"
     CDS             complement(3100..5439)
                     /locus_tag="VFF72_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV92924.1"
                     /translation="MTSTSRWAWIASTVAALAAGLVLAFLLSNATNDPSLYERHYVWL
                     FWLNVALASVLVLVIVVTGIRLLWRVSRGRFGSRLLLKLAAIFALVGVVPGLVIYTVS
                     YQFVSRSIESWFDVRVEGALDAGLNLGRGTIDSLVADLASKTRQSAERLAESSVPPDP
                     LALERLREQLSAQDVSIVGSAGQTLITTGPSVVGLMAERPSPQLLRQAHAQRVASIAE
                     GLDEDSAGNGNGGGAGHGARVRAVALIPSSNFALGEQDRYLVVTQSIAPSLAANALAV
                     TSAYREYQQRSLARSGLRKMYIGTLTLALVLAVFGALLLAVALSNQLAQPLLLLAEGV
                     SEVARGDFRSKPVFASRDELGGLTRSFAEMTEQLSEARELVHRSVVQAEGARANLQTI
                     LDNLTAGVMVFDRTGAIDTVNPGATRILRLPLSAYRGRRLEDVPGLGEFGQSIRQRFD
                     LHAASPEAGERDHWQDSFELKTTGPGGRERDSITLLVRGAAMPQGARLMVFDDITELV
                     SAQRVEAWSEVARRLAHEIKNPLTPIQLSAERMRLKLAGKLEGNDRGMLERSVETIVN
                     QVQAMKTLVNEFRDYARLPATHLVALDLNALVGEVLGLYGSAQESGRLHAELGQALPG
                     IVGDGTQLRQVIHNLVQNALDAVAERDDGRVRLFTEAARNEAGEVRAVRLVIVDNGPG
                     FSEKVLKRAFEPYVTTKTKGTGLGLAVVKKIADEHGARIRIVNLGSGEGGVDRPGRGE
                     RGVDDAAAGARGAQVSLSFSKLAPSLHAGAAEAAHASPP"
     gene            complement(5474..6088)
                     /locus_tag="VFF72_06885"
     CDS             complement(5474..6088)
                     /locus_tag="VFF72_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZV92925.1"
                     /translation="MTRTPPPGSLRRRSLLQALGVAALRGVPAIPAAAGLGSFTPVAG
                     AVPATITNFEVSRDEDGVYLAFSVDFELGRSVEEALMKSVPLFFVAEVEIFRDRWYWR
                     DRRVAHAVRTWRIVYQPLTSTYRVTTVGGLSQSFTSRSDALAAISRAARWKIAEPGQI
                     EDGGRHYLEFQYRLETAELPRPMQIGISGEPDWQLSVKRTLRLH"
     gene            complement(6085..>7399)
                     /gene="rsmB"
                     /locus_tag="VFF72_06890"
     CDS             complement(6085..>7399)
                     /gene="rsmB"
                     /locus_tag="VFF72_06890"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZV92926.1"
                     /translation="PLAVLLGHLAEAIAGVRRGTSLNELLARCPAEVRPGTQALAFHA
                     LRWLGSAEVVRRTLAARTPPPEVDALLLGALALLWPPSTPPYPDHVLVDQAVTAARRR
                     MPAGAAFVNAILRRFVREREGVVAAASAQPLARWNHPAWWLDRLRHDWPDDWQAIAEA
                     DNRHPPMTLRVNARRTGVAAYLERLARAGIAAEGVGGQAVNLRDPVPVTRLPGFAEGD
                     VSVQDAAAQRAAELLVAAPLAAGARVLDACAAPGGKTAHLLEIADLDVLAIDRDAARL
                     ERVDQTLARLGLSARTLAADAAEPESWCDGRPFEAILLDAPCSASGIARRHPDVRWLR
                     RSSDIAALAATQARLLDALWPTLAPGGRMLYCTCSLFREEGHAQIDAFLQRRPEAFVP
                     VSPPSPGHLLPLADNRRTRPAPASSATADGFFLTLIEKHRDLPIP"
BASE COUNT         1163 a         2539 c         2657 g         1040 t
ORIGIN      
        1 ggacgaccga catcctggcc cggctgatcg cgcaaaagct cggccccgcc ctgggcacct
       61 cgatcatcat cgagaacaag ggaggcgccg gcggcagcgt cggctcggag gtggcgtcgc
      121 gcgccgcgcc cgatggctac acgatcgtcg gcggaacgat cagctcgcat gccatcaacg
      181 tcagcctgta tcccaggatc ggctacgacc cggtgaagtc gttcgagccg atcgccctga
      241 tcggcaccaa cccgaccgtc ctcgtcgtca accaggcgag cccctacaag acgctcgccg
      301 acgtgatcgc ggccgccaag gccggcaagc acctgaccgc cgcctcggcc ggcagcggca
      361 cctcgcagca cctgtcgctc gagctgctga aggccaaggc cggcatcgac atcacgcaca
      421 tcccctacaa gggcagcggc ccggcgatcc aggacgtgat ggcggggcag gtcgacatga
      481 tgttcgacac caccgtcgtc gccggaccgc acatcgagag cgggcggctg cgcgcgctcg
      541 ccgtcacctc ggcccggcgc ctgccgtcga tgcccaacgt gccgaccgtc gccgaaaccg
      601 tgcctggcta cgaggtgatc tcgtggcagg ccatcttcgc gccggccggc acgccgcggt
      661 cgatcgtcga gcggctgcac gacgcgatcg tgaagatcct cgccgaaccc gacatccagg
      721 cgcggatgga caagctcggc atgcagggcg cgcacatgac gaccgccgaa gtccttgcct
      781 tccagaaggc cgaggtcgcg aagtgggccg ccgtgatcaa ggcggcgcac atcaaggtcg
      841 agtagcgggg gccgtcggtc aaccgccggc cgcgagctcg cgcagccacg cctcgccgcg
      901 cggcgagaag gcgacgacac gcgagccggc ggcgcgtcga ccccagcgca ggtcgaacag
      961 ccgggccagc agcgccgcgc cgaccgcgcc gccgaggtgg tggcggcgct cgccccagtc
     1021 gaggcacgcc cggcacgccg gccggcgctg cgcgagcacc gcgccggcgt cgacaccgaa
     1081 cgcgtcgagc caggaccggc cggcgcgcgt cagccgcagg ccgtcctcgc cgcccgcgag
     1141 atagcggcgg cgcagcaggc cgtcgtagag aaggacgccg agttcgccgg ccaggtggtc
     1201 gtagcagacc cgggccttgc gcagggccgg ctcgcgcggg ccggtgacga gacgaacggc
     1261 accggtgcga aacgcgacgc ccatcagcga ctcgagcagg tcggcgacgt cgcgatcggc
     1321 gagccggaag tagcggtgcc ggccctgctg ctcgacggcc agcaggccgg cgtcgagcag
     1381 ctgcgcgaga tgaccgctga tcgtctgctt cgtgaccccg gccaggccgg ccagctcggt
     1441 cggcgtcagg gcccgaccgg cgagcagcgc cgacagcgtc tcggcgcgaa ccggatcgcc
     1501 gatcaacgcg gcgacgcggg cgatgttcgg tccgtcgctc atgcgggtcg atctctcatg
     1561 attcgatcgt agccgaaccg tggtcgccat gggcgcattg cgttgcgcgg acgggagagg
     1621 cggcgaaacg tcgcccgcac ccgcgcgatg gctcttgccg atatcacaca aaaggccgct
     1681 gcgcccgctt cgtcaaggct cgcgaacgaa ggaccttcgg gaaacggcga ctgtcgtgcg
     1741 caagttccgt tcgccgcggg attgacgcgc gatgcgctca acattgcagg cgtggcgccg
     1801 aagaagaggg cgcccggctc acgcagagca tcgggctacc cattcattcg tgtggagaac
     1861 gctcttgtcg accttgtatt tcctggcctt ttgcggtgtc tgcgctgtcg tgctcggcac
     1921 gatgatcgac accatcatca aggtgaccag agccccggac tggaacgtcg gcaggccatt
     1981 ttccatcggg caggagacgg tcgcgctgat ccccatcgcg tcccatgacg tcgagccgcg
     2041 ccattcgggc ttcgtcggcc tgacgaacga cgacgaattc cagatgaccg cctgaggcgg
     2101 gaggcacgac gccgcgccgc cagcggcggc gtcgcgtgcc gtcaagacgc acgacggcct
     2161 gcgtcatgcg tccccatggc gccggaggat cgcgccgaca cggggtgcag gcatcgtcgc
     2221 gtctgatgcc gagccgaggc cgagcaccca aaccttgggc gtcgcgattc aagcgccggg
     2281 aacgtctggt ggccaagggc ggaatcgaac caccgacacg cggattttca gtccgctgct
     2341 ctaccgactg agctacttgg ccgtgagccg gcgattatat cggcggccct tcgcagcgcc
     2401 ggcgttcaga ccgggctgcg cttgttgcgc gacaggtcga cgccgagctg cttgagcttt
     2461 cgatacaggt gggtgcgctc gaggccggtc ttttcggcga cgcgcgtcat gctgccgttt
     2521 tcctgggcga ggtggaattc gaagtagctc ttctcgaagg catcgcgcgc ttcgcgcaac
     2581 ggccggtcga ggtcgaagct ctgctcgggt tgtgggccca gcatcaccgg catcgacggc
     2641 ggcatcgcgc cgcccgtcat cgctgcttcg acggtgaggc ccgcctcgct cgccccggcg
     2701 ggcgcggcga cagcggcggc acgcgaggcc ggcttggcga ggccctgctc gacggcgcgc
     2761 aggagctttt gcaacgtgat cggcttttcg agaaaggcga tcgccccgaa cttggtcgct
     2821 tcgaccgcgg tgtcgatcgt gccgtgcccg ctcatcatga tcacgggcat ggtcagcagc
     2881 gagctggcgc cccattcctt gagcagcgtg atgccgtcga catcgggcat ccagatgtcg
     2941 agcagcacga ggtcgggccg gaaccgctcc cggcaggcgc gtgcctgcgc cgcatcctcg
     3001 gccagctcga ccgtgtggcc ctcgtcggcg aggatctccg acagcaatgc gcgaatgccg
     3061 agttcgtcgt cgacgacaag aatggtggcc atgaaccttt cagggaggtg acgcgtgcgc
     3121 cgcctcggcc gccccggcgt gcaacgacgg agcgagtttt gaaaatgata acgaaacttg
     3181 cgccccgcgc gcgccggcgg ccgcatcgtc gacgcctctc tctccgcgcc ccggccgatc
     3241 gacaccgcct tcgccgctgc cgagattgac gatgcggatt cgcgcgccgt gctcgtcggc
     3301 gatcttcttg acgaccgcga ggccgagacc ggtgcccttg gtcttggtgg tcacgtaggg
     3361 ctcgaatgcg cgcttgagaa ccttctccga aaagcccggc ccgttgtcga cgatgacgag
     3421 tcgaacggca cgcacctcgc cggcctcgtt gcgcgcagct tcggtgaaga gccggacccg
     3481 cccgtcgtcg cgctcggcga cggcgtcgag ggcgttctgc accaggttat ggatgacctg
     3541 gcgaagctgg gtgccgtcgc cgacgatgcc gggcagggcc tggccgagct cggcgtgcag
     3601 ccgccccgat tcctgcgccg agccgtagag gccgagcacc tcgccgacca gcgcgttgag
     3661 gtcgagagcg acgaggtgcg tcgccggcaa ccgggcgtag tcgcgaaact cgttgaccag
     3721 cgtcttcatc gcctgcacct ggttgacgat ggtctcgacc gatcgctcga gcatgccgcg
     3781 gtcgttgccc tcgagcttgc cggccagctt gaggcgcatc cgctcggccg agagctggat
     3841 cggcgtcagc gggttcttga tctcgtgggc gagccggcgg gccacttcgc tccacgcctc
     3901 gacgcgctgc gccgagacga gctcggtgat gtcgtcgaac accatgagac gggcgccttg
     3961 cggcatcgcc gcgccgcgca cgagcagggt gatcgaatcg cgctcgcggc ctcccgggcc
     4021 ggtggtcttc agctcgaacg aatcctgcca gtggtcgcgc tcgccggcct cgggactggc
     4081 ggcgtggagg tcgaagcgct gccggatcga ctggccgaat tcgccgagtc cgggcacatc
     4141 ctcgaggcgg cgaccgcgat aggccgacaa gggcaggcgc aggatgcggg tcgctcccgg
     4201 gttgacggtg tcgatggcgc cggtgcggtc gaagaccatc acgccggcgg tcaggttgtc
     4261 gaggatggtc tgcaggttgg cgcgcgctcc ctcggcctgg acgacgctcc gatgcaccag
     4321 ctcgcgtgcc tccgaaagct gctcggtcat ctccgcgaac gaacgggtca gaccgccgag
     4381 ctcgtcgcgc gaggcgaaca ccggcttgga gcgaaagtcg ccacgcgcga cttcggagac
     4441 gccttcggcc agcagcaaaa gcggctgggc cagctggttg ctgagggcga cggcgagcaa
     4501 aagggcgccg aagaccgcga gcaccagcgc cagcgtcagc gtgccgatgt acatcttgcg
     4561 caagcccgag cgcgccagcg agcgctgctg gtattcgcgg tacgcgctcg tcaccgccag
     4621 ggcgttggcg gcgagcgaag gcgcgatcga ctgggtgacg accaggtagc gatcctgttc
     4681 gccgagcgcg aaattgctgc tcgggatcag ggccacggca cgcacccgcg cgccgtggcc
     4741 ggcgccaccg ccgttgccgt tgccggcgct gtcttcgtcg agaccttccg cgatgctggc
     4801 aacgcgctgc gcgtgggcct ggcgcaagag ctgcggcgac ggtcgctcgg ccatcagccc
     4861 gacgacgctg ggcccggtcg tgatcaaggt ctgcccggcg ctgccgacga tgctcacgtc
     4921 ctgcgccgaa agctgctcgc gcagccgctc cagggccagc gggtcgggcg gcacgctgct
     4981 ctcggcgagc cgctccgccg actggcgcgt cttgctggca agatcggcga cgagcgagtc
     5041 gatcgtgccg cggccgagat tcaggccggc gtcgagcgcg ccttcgacgc gtacgtcgaa
     5101 ccagctctcg atcgagcgcg acacgaactg gtagctcacc gtgtagatga cgaggccggg
     5161 aacgacgccg acgagggcga agatcgctgc cagcttgagc agcaggcgac tgccgaagcg
     5221 gccacgcgag acgcgccaca gcaagcggat cccggtgacg acgatgacca ggacgagaac
     5281 gctggccagc gcgacgttga gccagaacag ccacacgtag tggcgttcgt agagcgacgg
     5341 gtcgttggtg gcgttcgaga gcaggaacgc gagcacgagg ccggcggcca gcgccgcgac
     5401 ggtcgacgcg atccaggccc agcggctcgt cgaggtcatg gccggcggcg cgccgcgcag
     5461 gaagctgtcg tcttcaatgc agtcgcaagg ttcgcttgac cgagagctgc cagtcgggct
     5521 cgccgctgat gccgatctgc atcggccgcg gcagctcggc cgtctcgagc cggtactgga
     5581 actcgagata gtgacggccg ccatcctcga tctggcctgg ctcggcgatc ttccagcgcg
     5641 ccgcccggct gatcgcggcg agcgcatcgc ttcgcgaagt gaagctctgg ctgaggccgc
     5701 ccaccgtggt gacgcgatag gtcgacgtca gcggctggta gacgatgcgc cacgtccgca
     5761 ccgcatgggc gacccgccga tcgcgccagt accagcggtc gcgaaagatc tcgacttcgg
     5821 caacgaagaa gagcggcacc gatttcatga gcgcttcctc gacgctgcga ccgagctcga
     5881 agtcgacgga aaaggcgaga tagacgccgt cctcgtcgcg gctgacctcg aagttggtga
     5941 tcgtcgcggg cacggccccg gcgaccgggg tgaagctgcc cagaccggcc gcggccggga
     6001 tcgccggaac tccgcgcaac gccgcgacgc cgagcgcttg cagcaggctg cggcggcgca
     6061 acgagccggg cgggggcgtc cgggtcatgg aatcggcagg tctcggtgtt tctcgatgag
     6121 cgtcaggaag aagccatcgg ccgtggcgct cgacgcgggc gccggccgcg tccgtcgatt
     6181 gtcggccagc ggcagcagat ggcccggcga tggcggcgag acagggacaa aggcctcggg
     6241 ccgtcgttgc aaaaaagcgt cgatctgcgc atgtccctct tccctgaaca gcgagcacgt
     6301 gcagtacagc atccggccac cgggtgccag cgtcggccag agggcgtcga gcaggcgcgc
     6361 ctgggtggca gccagggcgg cgatgtcgct gctccggcgc aaccagcgca cgtccggatg
     6421 gcggcgcgcg atgcccgacg cgctgcaggg tgcatcgagg aggatcgcct cgaacggccg
     6481 gccgtcgcac cagctttcgg gctcggcggc gtcggccgcg agagtgcgtg cgctcaggcc
     6541 gaggcgggcc agcgtctggt cgacgcgctc gagccgggcg gcgtcgcggt cgatcgccag
     6601 cacgtcgagg tcggcgatct cgagcaggtg cgcggtcttg ccacccggcg cggcacaggc
     6661 gtcgaggacg cgcgcgcccg cagcgagcgg cgccgcgacg agcagctcgg cggcccgctg
     6721 cgccgccgcg tcctgcaccg aaacgtcgcc ctcggcgaag ccgggcaggc gcgtgaccgg
     6781 cacggggtcg cgaaggttca ccgcctggcc gccgacgcct tcggcggcga tgccggcccg
     6841 cgccagtcgc tcgagatacg cggcgacgcc ggttcgccgg gcgttgacgc gaagggtcat
     6901 cggcggatgc cggttgtcgg cctcggcgat cgcctgccag tcgtcgggcc agtcgtgccg
     6961 cagccggtcg agccaccacg ccggatgatt ccaccgcgcc agcggttgcg ccgatgccgc
     7021 ggcgacgacg ccctcgcgct cgcggacgaa gcggcgcagg atggcgttga cgaatgcggc
     7081 gccggccggc atgcggcgac gtgccgccgt gaccgcctgg tcgaccagca catggtcggg
     7141 atagggcggc gtcgaaggcg gccagagcag ggccagcgcc cccaggagca gcgcatcgac
     7201 ctcgggcggc ggcgttcgtg ccgcgagggt ccggcgcacg acctcggccg agccgagcca
     7261 gcgaagcgcg tggaacgcca gcgcctgcgt gccggggcgg acctcggcgg ggcagcgggc
     7321 gagcaactcg ttcagcgatg tgccgcggcg cacgccggcg atcgcttccg cgagatgacc
     7381 gagcaggacg gccagcggc
//
LOCUS       DASRWS010000365         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3331042, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000365 DASRWS010000000
VERSION     DASRWS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331042"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFF72_06895"
     CDS             <1..711
                     /locus_tag="VFF72_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZV92927.1"
                     /translation="LNALTLPMWQRLGEAMRELAADDGLRCIVVRGAGGRAFAPGNDI
                     GEFASTRSNFAQGKAYGKVLHEALGAVADCRHPTVALIEGICVGGGLEIAALCDLRIC
                     GESSRFGVPINKLGLVMAHAELAALVQLAGRATALEILLEGRVFGAREALEKRLVNRV
                     VADAEVADEAYATARRIAAGAPLVARWHKRFLRQIESAATLTEGERDEGFRCYDSEDF
                     GIGYRAFLARTVPEFVGR"
     gene            764..1984
                     /locus_tag="VFF72_06900"
     CDS             764..1984
                     /locus_tag="VFF72_06900"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZV92928.1"
                     /translation="MKTVDLPPTTGPLAGLVVLELAQIMAGPTCGSLLADLGADVIKI
                     ERIPGGDDTRRYAEPQVRGESAAFMMMNRNKRGVAIDLKKAGGVRVLRRLAERADALV
                     ENYRRGALDRLGLGYAALQAINPALIYCSISGYGRTGPAADKGGFDLIAQGASGLMSI
                     NGEPGGPPVKVGNPVTDLNAGMLAALGIVSAYVHRLKTGQGQLVDTSLFEAGIHQTAW
                     QAAIFFATGRSPGPTGSAHVLAAPYQAFATADGWINIGGANQANWERVARLVGAPELT
                     GDARFIDNAARMANRDALVALLTEKLRTRPTRDWLAELDAAGVPAGPIQSIAGMAADP
                     QTLAREMVVTLEHPVAGETRVLGVPVKLGATPGNIRRPAPTFGQHTREVLEEAGFAAD
                     EIEALAAEEAVHLG"
     gene            complement(2004..3299)
                     /gene="meaB"
                     /locus_tag="VFF72_06905"
     CDS             complement(2004..3299)
                     /gene="meaB"
                     /locus_tag="VFF72_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZV92929.1"
                     /translation="MPTAEALTTQALTGDRRAIARLLTLFDDDGPGARGAAALLAARA
                     GRARVVGITGVPGSGKSSLVNALLGAWLGRGLTVAVVAIDPSSPISGGAVLGDRIRMA
                     AHGAHPNAFIRSLSARGELGGLSRAARAAVDCFDAAGFDRVIVETVGTGQSETRIAAL
                     ADTRIVVCPPGLGDDVQALKAGTLEIGDVLAVSKGDLPLAAQTAREMREMLGLRRAAG
                     PAATWTARVVVVKSLEAGGIDGLLEAIEDHAQATDRGRRLRDEAMTPSSPAPTAPPAP
                     TAPTAASTTRSRDIPAACEAEAWRGRLAALAERDGLCTTLGISLVAGGAGSAETVMTV
                     DARHVNFNGACHGGAIFALADSAFGLASNSHGPVAAGIDAHITYQAGVAAGDRLVARA
                     SEVHRTRRLAVYRIDVVRPEPDGDETAISSFTGTVYIKG"
     gene            complement(3342..>3748)
                     /locus_tag="VFF72_06910"
     CDS             complement(3342..>3748)
                     /locus_tag="VFF72_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZV92930.1"
                     /translation="DDAGGMYKAVEAGLVQRMIGASALRFQQRIESGEQVVVGVNAYR
                     ADEDRTTLPAVERPDAAAMRRHLDDFAAWKAARPAAAVEAALDGLTRAANDDRLNVFG
                     AVVDAARAGLTNEEICGRLRRDLGFGQPLAFV"
BASE COUNT          514 a         1360 c         1369 g          505 t
ORIGIN      
        1 ctcaacgcgc tgacgctgcc gatgtggcaa cgactcggcg aggcgatgcg cgagctcgct
       61 gccgacgatg gactgcgctg catcgtcgtg cgcggcgccg gcggtcgcgc cttcgcaccg
      121 ggcaacgaca tcggcgagtt cgccagcacg cgctcgaact tcgcccaggg caaggcctac
      181 ggcaaggtgc tgcacgaagc gctcggcgcg gtcgccgact gccgtcatcc gacggtggcc
      241 ctgatcgagg gcatctgcgt cggcggcggc ctcgagatcg cggcgctgtg cgatctgcgc
      301 atctgcgggg aatccagccg cttcggcgtg ccgatcaaca agctcggcct ggtcatggcg
      361 cacgccgagc tggcggcgct ggtgcagctc gccggccgcg ccacggcgct cgagatcctg
      421 ctcgagggtc gtgtcttcgg cgcccgcgag gcgctggaaa agcgcctcgt caaccgcgtc
      481 gtcgccgatg ccgaggttgc cgacgaagcc tacgcgacgg cgcggcgcat cgccgccggt
      541 gcaccgctcg tcgcccgctg gcacaagcgc ttcctgcgcc agatcgaatc ggccgcgacg
      601 ctgaccgagg gcgagcggga cgaaggcttt cgctgctacg acagcgagga cttcggcatc
      661 ggctaccgcg ccttcctcgc caggaccgtg ccggagttcg tcggccggtg agcggcgcgg
      721 cgcgctttcg cgcccggctg aggcggggtg accggacacg tcgatgaaga ccgtcgatct
      781 accgccgacg accgggccgc tcgccggtct tgtcgtcctc gagctggcgc agatcatggc
      841 cggaccgacc tgcggctcgc tgctcgccga cctcggcgcc gacgtcatca agatcgagcg
      901 gatccccggt ggcgacgaca ccaggcgcta cgccgagccg caggtgcgcg gcgagtcggc
      961 cgcgttcatg atgatgaatc gcaacaagcg cggcgtcgcg atcgacctga agaaggccgg
     1021 cggcgtgcgc gtgctgcgcc gactggcgga gcgcgccgac gcgctggtcg agaactaccg
     1081 gcgcggcgcc ctcgaccgcc ttggactcgg ctacgccgca ctgcaggcga tcaacccggc
     1141 gctcatctat tgctcgatct cgggctacgg gcgcacgggg ccggccgccg acaagggcgg
     1201 cttcgacctc atcgcccagg gcgcgtcggg cctcatgagc atcaacggcg agccgggggg
     1261 accgccggtc aaggtcggca atccggtgac cgacctcaac gccggcatgc tcgccgcgct
     1321 cggcatcgtc agcgcctacg tgcatcgcct gaagacgggc caaggccagc tcgtcgacac
     1381 ctcgctgttc gaggccggca tccaccagac ggcgtggcag gcggcgatct tcttcgcgac
     1441 cggccgctcg cccggcccga ccggctccgc gcacgtcctc gccgcgccgt accaggcctt
     1501 tgccacggcc gacggctgga tcaacatcgg cggcgccaac caggcgaact gggagcgcgt
     1561 cgcgcgtctg gtcggcgcgc cggagctgac cggcgacgcc cgcttcatcg acaacgcggc
     1621 gcgcatggcg aatcgcgacg cgctggtggc gctgctcacc gaaaagctgc gcacgcggcc
     1681 gacgcgcgac tggctcgccg agctcgacgc cgccggcgtt ccggccggac cgatccagtc
     1741 gatcgccggg atggccgccg atccgcagac gctggcgcgc gagatggtcg tcacgctcga
     1801 gcatccggtc gccggcgaga cgcgcgtgct cggcgtgccg gtcaagctcg gggcgacgcc
     1861 aggcaacatc cgacgtccgg caccgacctt cggccagcac acgcgcgaag tcctcgagga
     1921 ggccggcttc gccgccgacg agatcgaggc gctcgccgcc gaagaggccg tccacctcgg
     1981 ctgagctcgg ccactcgtcg ccgtcagccc ttgatgtaga cggtgccggt gaagctggag
     2041 atcgccgtct cgtcgccgtc cggttccggg cgcacgacgt cgatgcggta gacggcgaga
     2101 cgacgcgtcc ggtgcacttc gctcgcccgt gcgacgagac gatcccccgc ggcgacgccg
     2161 gcctggtagg tgatgtgcgc gtcgatgccg gcggcgaccg ggccatgtga attcgaggcc
     2221 agcccgaacg ccgagtcggc gagcgcgaag atggcgccgc cgtgacaggc gccgttgaag
     2281 ttcacgtgcc gcgcatcgac ggtcatcacc gtctcggcac tcccggcgcc gccagcgacg
     2341 agcgagatgc cgagcgtggt gcagaggccg tcgcgttcgg cgagcgcggc gaggcggccg
     2401 cgccaggcct cggcttcgca ggcggccgga atgtcgcggg accgggttgt cgaggcggcc
     2461 gtcggcgcag tcggcgcagg cggcgcagtc ggcgccggcg acgacggcgt catcgcttcg
     2521 tcgcgcaagc gtcgcccgcg atccgtcgcc tgcgcgtggt cctcgatggc ttcgagcagg
     2581 ccgtcgatgc cgcccgcctc cagcgacttg acgaccacca cccgcgccgt ccaggtcgct
     2641 gccggacctg cggcacggcg caggccgagc atctcgcgca tttcgcgcgc cgtctgcgcc
     2701 gccagcggca ggtcgccctt gctgacggcg agcacgtcgc cgatctcgag cgtgccggcc
     2761 ttgagcgcct gcacgtcgtc gccgaggccg ggcgggcaga cgacgatgcg cgtgtcggcc
     2821 agcgccgcga tgcgcgtttc cgactggccg gtgcccacgg tctcgacgat gacgcgatcg
     2881 aaaccggcgg cgtcgaagca gtcgacggcg gcgcgcgccg cgcgcgacag tccgccgagc
     2941 tcgccacgtg ccgacagcga gcggatgaag gcgttgggat gggcaccgtg cgcggccatg
     3001 cggatgcgat cgccgagcac ggcgccgccg ctgatcgggc tcgacggatc gatggcgacg
     3061 acggccaccg tcaggccacg cccgagccag gcgccgagca gggcgttgac gaggctcgac
     3121 ttgccgctgc cgggcacgcc ggtgatgccg acgacgcggg cccgtccggc gcgcgcggcg
     3181 agcagggcgg cggcgccgcg cgcgcccggc ccgtcgtcgt cgaagagcgt cagcaggcgg
     3241 gcgatggcgc ggcggtcacc ggtcagcgct tgcgtcgtca gcgcttcggc ggtcggcatg
     3301 gccgcgttcg cgttcgcggt ggcgatgggc gcagccacgc gtcagacgaa ggccagcggc
     3361 tggccgaagc cgagatcgcg gcgcagccgg ccgcagatct cctcattcgt gagcccggcc
     3421 cgcgctgcgt cgacgacggc gccgaagaca ttgagcctgt cgtcgttcgc ggcgcgcgtc
     3481 agcccgtcga gcgcggcctc gaccgcggcc gcggggcgcg ccgccttcca tgccgcgaag
     3541 tcgtcgagat ggcgtcgcat cgccgccgca tcgggtcgct cgaccgccgg cagcgtcgtc
     3601 cggtcttcgt cggcgcgata ggcattgacg ccgacgacga cctgctcgcc gctctcgatg
     3661 cgttgctgga agcgcagcgc cgaggcgccg atcattcgtt gcacgaggcc ggcttccacc
     3721 gccttgtaca tgccgccggc gtcgtcga
//
LOCUS       DASRWS010000366         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3889426, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000366 DASRWS010000000
VERSION     DASRWS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889426"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFF72_06915"
     CDS             complement(<1..1147)
                     /locus_tag="VFF72_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZV92931.1"
                     /translation="MKTQTLAPDLLRKMDAYWRAANYLSVGQIYLYANPLLREPLTLA
                     HVKPLVVGHWGTTPGQNFIYTHLNRVIRKYDLDMFYIAGPGHGGPALVGSTYLEGSYS
                     EIYPDISQDEAGMQKLFKQFSFPGGISSHVAPSTPGSIHEGGELGYSLSHAFGAAFDN
                     PGLIVACIVGDGEAETGPLATAWQSTKLLDPITDGAVLPILHLNGWKISNPTLLARIE
                     HEELEQFFRGCGWTPYFVEGDDPDKMHELMATVLEAAVEDIRRIQAAARSSGDPARPR
                     WPMIVLRSPKGWTGPKVVDGLHIEGTFRSHQVPLLVDAAHPDHVKLLESWMKSYKAEE
                     LFDENGRLVAELAELAPLGDRRMGANPHTNGGSLLRDLRMPDFHEHAV"
     gene            1324..2481
                     /locus_tag="VFF72_06920"
     CDS             1324..2481
                     /locus_tag="VFF72_06920"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZV92932.1"
                     /translation="MNVLALNSGSSSLKFGLYDVRPESVELSVSGEAEAIGESGSRFH
                     AEDSRCVVVASKRGPIADQRGAVVHVAQLLATLGLPAPAAVGHRIVHGGPKLRRHCLI
                     DDSVLRELEAATPYAPLHMQPALAVVRFAEEHFPGTPQVACFDTAFHAAMPEVASTLP
                     IPLALRSQGIRRYGFHGLSCESIVRQLAGRLPDRLLIAHLGNGASVTAVRDGSSIDTS
                     MGLTPSGGLIMGTRSGDLDPGVLIYLLREKGFDADRLEDMVDHRAGLLGISGLAGDMR
                     RLHEQAAACADARLAIEMFCYSVRKELAAMTSVLGGVDAIVFTGGIGENDAAVRGAIC
                     AGLAWSGIRLDESGNRAGKRGLVSDASSSCEVHVLASKEDEQIARHTAALL"
     gene            2631..>2902
                     /locus_tag="VFF72_06925"
     CDS             2631..>2902
                     /locus_tag="VFF72_06925"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZV92933.1"
                     /translation="MSMLRSIKDLEEYAVGATDGTIGHVKDLYFDDQAWVVRYFVVDT
                     GAWLTGRKVLVSPIAIERSDWAERVLHVRMTKEQVKNSPDIDTDKPV"
BASE COUNT          457 a          948 c          994 g          503 t
ORIGIN      
        1 tcaccgcgtg ctcgtggaag tcgggcatgc gcaggtcgcg cagcagcgaa ccgccgttgg
       61 tgtgcgggtt ggcgcccatg cgccggtcgc ccaggggtgc cagctcggcc agctccgcca
      121 ccaggcggcc gttctcgtcg aacagctcct cggccttgta gctcttcatc cagctttcga
      181 gcagcttgac gtgatcgggg tgcgcggcgt ccacgagcag cggcacctga tgcgatcgaa
      241 aggtcccttc gatatgcaat ccgtcgacga ccttcgggcc ggtccagccc ttcggtgaac
      301 gcagcacgat catcggccag cgaggccggg ccgggtcgcc gctgctgcgc gcggcggcct
      361 ggatcctgcg gatgtcctcg acggctgctt ccagcaccgt cgccatcagc tcgtgcatct
      421 tgtccgggtc gtcgccctcg acgaaatacg gcgtccagcc gcagccacgg aagaactgct
      481 cgagctcttc atgctcgatg cgggcaagga gagtgggatt gctgatcttc cagccgttca
      541 ggtgcaggat cggcagcacg gcgccgtcgg tgatcggatc gagcagcttc gtcgactgcc
      601 acgcggtcgc cagtggcccc gtttcggctt cgccgtcgcc gacgatgcag gcgacgatga
      661 ggccggggtt gtcgaacgcc gcgccgaagg cgtggctgag cgaatagccg agctcgccgc
      721 cttcgtggat cgagcccggc gtcgacggcg ccacgtggct cgagatgccg ccgggaaacg
      781 agaactgctt gaagagcttc tgcatcccgg cttcgtcctg gctgatgtcg gggtagatct
      841 cgctgtagct gccctcgagg taggtgctgc ccaccagggc cggaccgccg tgccccgggc
      901 cggcgatgta gaacatgtcg aggtcgtact tcctgatgac ccggttcagg tgggtgtaga
      961 tgaagttctg tcccggcgtc gtgccccagt ggccgacgac caggggcttg acgtgcgcca
     1021 gcgtcagcgg ctcgcgcagc agcggattgg catagagata gatctgcccc acggacagat
     1081 agttggcggc gcgccagtag gcgtccatct tgcggagcag gtcgggcgcg agcgtctgtg
     1141 ttttcatgat tcaaaggttc tacggaccgc ggcgactgct gtctgtgcgg tagcggtcat
     1201 agccgtgtca aaaaaatagt gatcgtgcgc gcgaggttcg caacgcggcg aacacagaat
     1261 ggaacgcgcg aagcgaaggc agcgccaccg tctcttcttc cttccccact tcaggcagtc
     1321 gcgttgaacg ttctcgctct caacagcggt tcctcgtcgc tcaagttcgg cctctacgac
     1381 gtccgtcccg aatcggtcga gctgtccgtc tcgggcgaag cggaggcgat cggcgaatcg
     1441 ggcagccggt ttcatgccga agattcgcgc tgcgttgtcg tcgcgtcgaa gcgcggcccc
     1501 atcgccgacc agcgcggcgc cgtggtccat gtggcgcaac tgctcgccac gctcggcctg
     1561 ccggcgccgg ccgccgtcgg gcaccgcatc gtccacggcg gcccgaagct gcgtcgtcat
     1621 tgcctcatcg acgactcggt ccttcgcgag ctcgaggccg cgacgcccta cgcgccgctg
     1681 cacatgcaac ccgcactggc cgtcgtccgg ttcgcggagg agcacttccc cggcaccccg
     1741 caggtggctt gtttcgatac ggcgttccac gcggccatgc cggaggtggc gagcacgctg
     1801 cccattccgc tggcgcttcg ctcgcaggga atccggcgct acggattcca cggcctgtcg
     1861 tgcgaatcga tcgttcgcca gctggccggc cgtctgcccg accgtctgct catcgctcac
     1921 ctgggcaacg gcgccagcgt cacggccgtg cgcgacggga gctcgatcga caccagcatg
     1981 ggcctgacgc cgagcggcgg cctgatcatg ggcacgcgca gcggcgatct cgacccgggc
     2041 gtgttgatct atctcctgcg cgagaagggg ttcgacgccg atcggctcga agacatggtc
     2101 gaccatcgcg ccggcctcct cggcatctcg ggcctggccg gcgacatgcg gcggctccac
     2161 gagcaggccg cggcgtgcgc cgacgcgcgg ctggcgatcg agatgttctg ctactcggtc
     2221 aggaaagagc tggccgcgat gacgtcggtg ctcggcggcg tcgacgcgat cgtcttcacc
     2281 ggagggatcg gcgagaacga cgccgccgtg cgcggcgcga tctgcgccgg gctggcgtgg
     2341 agcgggatcc gcctcgacga atccggcaat cgggccggca aacgcggcct cgtcagcgac
     2401 gcgtcgtcga gctgcgaggt gcatgttctc gcgtcaaagg aagacgagca gatcgcgcgg
     2461 cacacggcgg cattgctctg acccctccgg ccggcgctgc ggccggcagg gcgaacggcc
     2521 cccgacgtca gggcgagtcg ctcatggggg catcgtcctg actatgttcg gtggcataca
     2581 gacgagcgca tgatggaacg cgcagagtgc agtgaatcat cgcaggagcc atgagcatgt
     2641 tgcgcagcat caaggatctc gaggaatatg cggtcggcgc caccgatggc acgatcggcc
     2701 acgtcaagga cctctatttc gacgatcagg cgtgggtggt gcgctatttc gtcgtcgaca
     2761 ccggtgcctg gctgacgggg cgcaaggtgc tggtctcgcc catcgccatc gagcggtccg
     2821 attgggccga gcgggtcctt cacgttcgga tgacgaagga gcaggtgaag aacagccccg
     2881 acatcgacac ggacaagccc gt
//
LOCUS       DASRWS010000367         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3033365, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000367 DASRWS010000000
VERSION     DASRWS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033365"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2003
                     /locus_tag="VFF72_06930"
     CDS             <1..2003
                     /locus_tag="VFF72_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZV92934.1"
                     /translation="QAILAAYPEGIAEGHDPALEGRYTLADGTPVAPSFQLLKERVAP
                     CTPEWAAQITGIAAARIVKLARELGETALHQAFEVPVAWTDAWGKRHETTQARPVAFH
                     AMRGLAAHSNGFQTIRGLAVLMSLLGTIDAPGGFRHKAPYPRHIVPNFRAFNSPEMIR
                     PNTPLNAAPLGFPAGPEELAIHDDGSPMRIDHAFSWEYPLSAHGLMHTVITNATRADP
                     YRIDTLLIFMANMAWNSTMNTMAVRAMLNQKNADGDFQIPFLVVCDAFQSEMVAFADL
                     VLPDTTYLEPHDVMSMLDRPISEFDGPCDSVRVPVLPPTGECKPFQEVIVELGSRLKL
                     PAFTTPEGQRRFKDYPDFVVNFEAAPGVGFLMGWRGKDGTEHLRGAPNPKQWEMYAKN
                     NCVFQYHLPESMHFMRNWNREYLDFAQARGWRQKNDVIQLQLYSDTLQKFRLAAQGKT
                     TGRQPPEHLRGRIETYFDPLPFWYPPLEVSATDTDAFPLAAITQRPMAMYHSWDSQNA
                     WLRQIHSHNYLQVNPRTAKDAGVADGGWCWVESPWGEVRCMARYSEAVEPGTVWTWNA
                     IGKADGAWQLAPGADESRKGFLLNHLITDELPGPAGQPVSNSDPVTGQAGWYDVRVRL
                     RPAQAEAHEETSPQIAANPAPPGVLGIGARPLAYFASKRRRP"
BASE COUNT          365 a          779 c          711 g          311 t
ORIGIN      
        1 cgcaagcgat tctggcggcc tatcccgaag gcatcgccga ggggcacgat ccggcgctcg
       61 aaggccgcta cacgctggcc gacggcacgc cggtggcgcc ctcgttccag ttgctgaagg
      121 agcgcgtcgc tccgtgcacg ccggagtggg cggcgcagat cacgggcatt gcggcagcgc
      181 gcatcgtcaa gctggcgcgc gagttgggcg agacggcgct gcaccaggcc ttcgaggtgc
      241 cggtcgcgtg gaccgacgcc tggggcaagc gccacgagac gacgcaggcg cggccggtgg
      301 cattccacgc catgcgcggg ctggccgcgc actcgaacgg ctttcagacg atccgcggcc
      361 tcgcggtgct gatgagcctg ctcggcacca tcgacgcccc gggcggcttc cggcacaagg
      421 cgccctatcc gcggcacatc gtgcccaact tccgcgcctt caattcaccc gagatgatca
      481 ggccgaacac gccgctcaat gcggcgccgc tcggctttcc ggccggcccc gaggaactcg
      541 cgatccacga cgacggctcg ccgatgcgca tcgaccacgc cttctcgtgg gagtacccgc
      601 tgtcggcgca cggcctgatg cacaccgtga tcaccaacgc gacgcgcgcc gacccgtacc
      661 gcatcgacac gctgctgatc ttcatggcca acatggcgtg gaactcgacc atgaacacga
      721 tggccgtgcg ggcgatgctc aaccagaaga acgcggacgg cgatttccag attcccttcc
      781 tggtcgtctg cgacgccttc cagagcgaga tggtcgcctt cgccgacctc gtcctgcccg
      841 ataccaccta tctcgagccg cacgacgtga tgagcatgct cgaccggccg atctccgagt
      901 tcgacggccc gtgcgattcg gtccgcgtgc cggtgctgcc gccgaccggc gagtgcaagc
      961 cgttccagga ggtgatcgtc gagctcgggt cgcggctgaa gctgcccgcc tttacgacgc
     1021 ccgagggcca gcgccggttc aaggactatc ccgacttcgt cgtcaacttt gaggcggcgc
     1081 cgggagtcgg attcctcatg ggctggcgcg gcaaggacgg caccgagcac ctgcgcggcg
     1141 cgcccaatcc gaagcagtgg gagatgtacg cgaagaacaa ctgcgtcttc cagtaccacc
     1201 tgcccgagtc gatgcacttc atgcgcaact ggaaccgcga gtacctcgac ttcgcccagg
     1261 ccagaggctg gcggcagaag aacgacgtga tccagttgca gctctactcc gacaccctgc
     1321 agaagttccg cctcgcggcg cagggcaaga cgacagggcg acagcccccc gagcacctgc
     1381 gcggacgcat cgagacctac ttcgacccgc tgcccttctg gtatccgccg ctcgaggtgt
     1441 cggccaccga caccgacgcc tttccgctgg ccgccatcac gcagcggccg atggcgatgt
     1501 accactcgtg ggactcgcag aacgcgtggc tgcgccagat ccacagccac aactacctgc
     1561 aggtcaaccc gcgcaccgcg aaggacgccg gcgtcgccga cggcggctgg tgctgggtcg
     1621 aaagcccctg gggcgaggtc cgctgcatgg cacgctacag cgaagccgtc gagcccggca
     1681 cggtgtggac ctggaacgcc atcggcaagg ccgacggcgc ctggcagctc gcccccggcg
     1741 ccgatgaatc acgcaagggc tttctcctca accacctcat caccgacgag ttgccggggc
     1801 cggccgggca gccggtcagc aattccgatc cggtcacggg gcaggccggc tggtacgacg
     1861 tgcgcgtgcg gctgcgcccg gcgcaggccg aggcgcacga ggaaacctcg ccgcagatcg
     1921 cggcgaaccc ggcgccgccg ggcgtcctcg gcatcggcgc caggccactg gcctatttcg
     1981 cctcgaagcg gaggcgtccg tgatcgagtg gctgaagaat ctgctgcagg aggacgcggt
     2041 cgcaccatct ccggcgccgg ctgaacgagg acgttcccca gcgcacgggc acgtctcggc
     2101 ggtcgtcgac gcggcgagca tcggcaccga tcacggtccg tcgacgaccc tggcccgcca
     2161 gctcgc
//
LOCUS       DASRWS010000368         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_454289, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000368 DASRWS010000000
VERSION     DASRWS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454289"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VFF72_06935"
     CDS             complement(<1..1290)
                     /locus_tag="VFF72_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZV92935.1"
                     /translation="MHVHAPVFKAYDIRGVFGRTIDDAFAEHLGRAFASEALAAGERA
                     VAVGRDGRLSGPSLSAALIRGLASTGIDVVDLGPVTTPMLYYVAATRGAHGCTSGVQV
                     TGSHNPKDDNGFKMVLAGRAIYGDDIQRLRRRMQDEDHVRGRAGRVGAMDIAAEYTAR
                     IAGDCRLARPMRVAVDCGNGIAGLTAPSVLRALGCVVDEIHSEVDGRFPNHHPDPSRP
                     ENLADLIALVARSGAEIGLAFDGDGDRLGVVTREGRIVYPDRQLMLLARDVLSRHRGA
                     TILFDVKCSQHLAPVIRAAGGEPVMWKTGHSLIKAKMKEISAPLAGEMSGHIFFGERW
                     YGFDDATYTAARLLEILARSADPGAVLDALPASFSTPELNVACGEGEAAEVVARLRDE
                     ARFDGAREIVDIDGVRVEYDDGFGLVRASNTTPVLVLR"
     gene            1436..>2275
                     /locus_tag="VFF72_06940"
     CDS             1436..>2275
                     /locus_tag="VFF72_06940"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZV92936.1"
                     /translation="MWDKQRIRESWRAWLSDDPARIGPWWAQAAWPIVFGTMCAIFFT
                     VFAVALNGNDAATHPSRWPSMFRVSLIISLSITLSVQLTFKLAAAAIGRPRLRAWSRP
                     RRSVYYLTLSLLGTAIGWPLGLYWALGIDVRQQFLTRRPDAVFAAIALAMLIAGLFAE
                     FFKIKSQQIRAENQATEARLRLLQAQIEPHFLFNTLANVVSLMDADTPRAKAMLESFV
                     DYLRASLVGFRHGTHTLGDEIDLVAAYLRIVKMRMEDRLQYTVDLAPELRAHALPPLT
                     LQPL"
BASE COUNT          316 a          816 c          783 g          360 t
ORIGIN      
        1 gcgaagcacc agcaccggcg tggtgttcga cgcgcgcacc aggccgaagc cgtcgtcgta
       61 ctcgacccgc acgccgtcga tgtcgacgat ctcgcgcgcc ccgtcgaatc gcgcctcgtc
      121 gcgcagccgc gcgacgacct cggccgcttc gccctcgccg cacgcgacgt tgagctccgg
      181 cgtgctgaag cttgccggca aggcgtcgag gacggcgccc gggtcggcgc tgcgggcgag
      241 gatctcgagc agccgtgccg ccgtgtaggt ggcgtcgtcg aagccgtacc agcgctcgcc
      301 gaagaagatg tgcccgctca tctcgccggc cagcggcgcg ctgatctcct tcatcttcgc
      361 cttgatcagc gaatggccgg tcttccacat gacgggctcg ccgccggcag cgcggatcac
      421 cggcgcgaga tgctgcgagc acttgacgtc gaagaggatc gtcgcgccac gatgcctgga
      481 caggacgtcg cgcgcgagca gcatcagctg ccggtcgggg tagacgatgc ggccttcacg
      541 cgtcacgacc ccgaggcggt cgccgtcgcc gtcgaacgcc aggccgatct cggcgccgct
      601 gcgcgcgacg agcgcgatca ggtcggccag gttttccggc cggctcggat cgggatggtg
      661 attggggaaa cgcccgtcga cctcggagtg gatctcgtcg acgacgcagc cgagcgcgcg
      721 cagcaccgac ggcgcggtga ggccggcgat gccgttgccg cagtcgacgg ccacgcgcat
      781 cggccgcgcc aggcggcagt cgcctgcaat gcgcgccgtg tactcggcgg cgatgtccat
      841 cgcaccgacg cgcccggccc gaccgcgcac atggtcttcg tcctgcatcc ggcggcgcaa
      901 tcgctggatg tcgtcgccgt agatggcacg gccggccagc accatcttga agccgttgtc
      961 gtccttcggg ttgtggcttc cggtcacctg gacgccgctc gtgcagccgt gcgccccacg
     1021 cgtcgcggcg acgtagtaga gcatcggcgt cgtcaccggg ccgaggtcga cgacgtcgat
     1081 tccggtcgag gccaggccgc ggatcagcgc cgccgagagc gacggccccg acaggcgccc
     1141 gtcgcgaccg accgcgacgg cccgttcgcc ggcggcgagt gcctcgctgg cgaaggcgcg
     1201 cccgagatgt tcggcgaagg catcgtcgat cgtgcggccg aagacgccgc ggatgtcgta
     1261 ggccttgaag acaggagcgt gaacgtgcat cgggaagcga gccggacggc tgcggcattg
     1321 tagggagcgc cctgcgcagc ctgcggctgc cgccgcgggc tggccagccg tcggcccggc
     1381 tgtcgatcgg tcgcagggag gcgccgcggc gattcgcgct tcgtacactg ccttcatgtg
     1441 ggacaagcag cggatacggg agtcctggcg cgcctggctg agcgacgacc ccgcgcgcat
     1501 cggcccgtgg tgggcgcagg cggcgtggcc gatcgtcttc ggcacgatgt gcgcgatctt
     1561 cttcaccgtc ttcgccgtcg cgctcaacgg caacgatgcg gcgacccacc cgtctcggtg
     1621 gccctcgatg ttccgcgtca gcctgatcat cagcctgtcg atcacgctct cggtgcagct
     1681 gacgttcaag ctcgccgcgg cggcgatcgg ccgcccgcgc ttgcgcgcct ggagcaggcc
     1741 gcggcgctct gtctactacc tcacgctgtc gctgctcggc acggcgatcg gctggccgct
     1801 cgggctctac tgggcgctcg gcatcgacgt gcgccagcag ttcctcaccc gtcgccccga
     1861 cgccgtcttt gccgccatcg ccctggcgat gctgatcgcc ggcctcttcg ccgagttctt
     1921 caagatcaag tcgcagcaga tccgggcgga gaaccaggcc accgaggcgc gcctgcgcct
     1981 gttgcaggcg cagatcgagc cgcacttcct cttcaacacg ctggccaatg tcgtctcgct
     2041 catggatgcc gacacgccgc gcgccaaggc gatgctcgag tcgttcgtcg actatctgcg
     2101 tgcgtccctc gtcggctttc gccacggcac gcatacgctg ggcgacgaga tcgacctggt
     2161 cgcggcctat ctgcgcatcg tcaagatgcg gatggaagac cggctgcagt acaccgtcga
     2221 ccttgcgccg gaactgcgcg cgcacgcgct gccgcccctc accttgcagc cgctg
//
LOCUS       DASRWS010000369         6171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2165488, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000369 DASRWS010000000
VERSION     DASRWS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165488"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFF72_06945"
     CDS             <1..209
                     /locus_tag="VFF72_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZV92937.1"
                     /translation="MLVHGEITSALAATLVERLQRVGQDFARAHVAEQKLPDEQRTPY
                     TLLVGMRSWLFAAFRDLKRSSTPN"
     gene            complement(264..1106)
                     /gene="rlmJ"
                     /locus_tag="VFF72_06950"
     CDS             complement(264..1106)
                     /gene="rlmJ"
                     /locus_tag="VFF72_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZV92938.1"
                     /translation="MLAYRHAFHAGNHADVLKHLVLMLVLKHMNDKPKTYRFVDTHGG
                     AGGYSLQGRYARKKGEYEEGIGRLWTREDLPGPVADYVALVRRFNPDGVLAQYPGSPA
                     FAQMLLRPHDQLRAFELHPTEQKILRSTLAATPDAIAYSGDGFDGLKSQLPPSSRRAA
                     VLIDPSYEGNGDYGRVVATLRDALGRFAPGVYMVWYPQVSKVEAVQLPRRLAALAPAS
                     WLDVRLTVQEPDRHGFGLAGSGVFLLNPPHTLRAHLERVMPFLVDALAQFDGARFAIE
                     SASA"
     gene            complement(1125..1712)
                     /locus_tag="VFF72_06955"
     CDS             complement(1125..1712)
                     /locus_tag="VFF72_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZV92939.1"
                     /translation="MKTGRLLSLNTALARPVSINGRRVKTAIAKQPVDGERGVFTLGI
                     EGDEQADLSVHGGVGKAVYAYPSEHYAFWQTVRAQAKVALWDEPLAFGSIGENLTLEG
                     VTEIDLWIGDVLQFPGCELAVSEPRIPCFKLNAALGFAHAARLMTQSGWCGAYLAVRS
                     PGTIAAGQPFTLRPGPREVGIVELFRARSGRATVA"
     gene            complement(1709..2521)
                     /locus_tag="VFF72_06960"
     CDS             complement(1709..2521)
                     /locus_tag="VFF72_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZV92940.1"
                     /translation="MHRRRLLAASLAATAVTANPGLAIAAQTPLRLGVDRALAESGLA
                     HALQHAFGADTGLAVKLAPGPALAILDAVKEGEVDAALTNVPAVETDLDAQGLVYDRQ
                     TIATGEFILVGPLPRTRGRPPPPGQSGVDALARIQEMATAEPGTMLFLSAGDGSGTHV
                     VEQALWRAAHIDPAAPWYVAADAKLPFIAQVRTRNAFALVEAGAWAVGGGPPEAVLVE
                     ADPMLGETVSAMRSFRAAHPAGKIFVAWISGGRGRAVVSRHRGYRPAAKKSR"
     gene            2744..4645
                     /locus_tag="VFF72_06965"
     CDS             2744..4645
                     /locus_tag="VFF72_06965"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZV92941.1"
                     /translation="MNRPQPIGATSRPPASLRHRKLAEWVEGMAALTEPSDIVWCDGS
                     DAEYDRLCARLVASGTLLKLNPELRPHSFLARSSASDVARVEDRTFICSEREEDAGPT
                     NHWMAPTEMRAILRDGRPDGTPALFKGAMRGRTMYVVPFSMGPLGSDIAHIGVELTDS
                     AYVAVSMKIMTRMGAKVLDVLGSDGHFVPCMHTVGAPLEPGQADSPWPCNPVKYIVHY
                     PESQEIWSYGSGYGGNALLGKKCLALRIASAMGRAAADSAKAEPGWLAEHMLVLGVTS
                     PEGRKHHVAAAFPSACGKTNFAMLIPPPSLAGWKVTTIGDDIAWIKPGADGRLFAINP
                     EAGYFGVAPGTNDKTNPNCMASLKHDAIFTNVALTDDGDVWWEGMTEQPPAHLIDWKG
                     QDWTPQIGRDTGAKAAHPNARFTVAATNNPALDPAWDSPAGVPIDAFIFGGRRSTTIP
                     LVTEARDWTEGVYMASTMGSETTAAAAGKQGVVRRDPFAMLPFIGYNMADYFQHWLDL
                     GAGLAKRGATLPKIFCVNWFRKGEDGKFVWPGYGENMRVLKWIVRRIEGSAGAELTAF
                     GWTPRYDDIDWSGLDFDRRRFDRVADLDSASWSGELELHDELFRKLAHGLPEALPKVR
                     RDIAQRLQR"
     gene            4663..5514
                     /locus_tag="VFF72_06970"
     CDS             4663..5514
                     /locus_tag="VFF72_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HZV92942.1"
                     /translation="MPESAPPSRPPPGAAAHDLTEAEFAELDGLLAGIPEPLEPLDVV
                     MLDGFLAGVVVQPLLLDVEQWLPYVFDSGGHRWGEAEPSPEQRRARELIVRRLAAINR
                     SLAEYRAFEPLILEPGDGSDEAADSQGEEAAEPGGGATIDVDSGDGAASPAAEAQDPA
                     ADPVTVAILPWVAGFQFAADLFPGLDEVPDDGVATALARLYRFLPAEEDDDRATVELV
                     RRERPLASIDAAVDEVVACVADLYDLTEPLRYRVETVRRAEPKVGRNDPCPCGSGRKF
                     KQCHGAG"
     gene            5525..>6171
                     /locus_tag="VFF72_06975"
     CDS             5525..>6171
                     /locus_tag="VFF72_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HZV92943.1"
                     /translation="MRLQLLSDLHLETEAFDPEPAPGAELLVLAGDIDSRWDGLARFA
                     GWPQPVLFVAGNHEFDGRDLDLGMAALRRRCEAVGITMLERESVVVEDDRGRPIRFVG
                     TTRWSDFDLFGESGRAKAMRAGEYFMALMKTSRDGAAFDAAAVRSEALICRNWLEAEL
                     ARPPGTWERTVVVTHFAPSLRSADPRYGERSSTASFCNADDDLLGRADLWLHGHL"
BASE COUNT          948 a         2131 c         2161 g          931 t
ORIGIN      
        1 tcatgctggt gcacggcgag atcaccagcg cgctggccgc gacgctggtc gagcgcttgc
       61 agcgtgtcgg ccaggatttc gcgcgtgccc acgtcgccga gcagaagctg cccgacgagc
      121 agcgcacgcc ctacacgctg ctcgtgggaa tgcgcagctg gctgttcgcc gcattcaggg
      181 acctgaagcg ctcgtcgacg ccgaactgag cggcgcgacc cgggcgcgcc atccgtcgat
      241 cggtcgatca gtcgaagggt cgatcaggcg ctggcgctct cgatcgcgaa ccgggcgccg
      301 tcgaactggg cgagcgcgtc gacgagaaag ggcatcacgc gttcgagatg ggcgcgcagc
      361 gtgtgtggcg ggttgagcag gaagacgccg ctgccggcca ggccgaaccc gtgacggtcg
      421 ggctcctgga cggtgaggcg aacgtcgagc cacgatgccg gcgcgagcgc ggcgaggcgc
      481 cgaggcagct gcaccgcctc gaccttgctc acctgcgggt accagaccat gtagaccccg
      541 ggtgcgaatc gacccagcgc gtcgcgcagc gtcgcgacga cccgcccgta gtcaccgttg
      601 ccctcgtagc tcgggtcgat caacacggcg gcgcgccgac tggagggtgg cagctgcgat
      661 ttgaggccgt cgaagccgtc gcccgagtag gcgatcgcgt cgggcgtcgc cgccagggtc
      721 gagcgcagga tcttctgctc ggtgggatgc agctcgaacg cgcgcaactg atcgtgcgga
      781 cgcagcagca tctgcgcgaa agccggcgaa cccgggtatt gcgccaggac gccgtcaggg
      841 ttgaagcgcc gtaccagcgc gacgtagtcg gcaaccgggc cgggcaggtc ttcgcgcgtc
      901 cagagccggc cgatgccctc ttcgtactcg cccttcttgc gggcataacg accctgcagc
      961 gaatagccgc cggcgccgcc gtgggtgtcg acgaaccgat aggtcttcgg cttgtcgttc
     1021 atgtgcttca ggaccagcat cagcacgagg tgcttgagga cgtcggcgtg gtttccggcg
     1081 tggaaagcgt ggcgataggc gagcatcggc agcgacgcgg cgtgttacgc cacggtggcg
     1141 cgaccggacc gggcgcgaaa cagttcgacg atgcccacct cgcggggccc gggccgcagc
     1201 gtgaaaggct ggccggcagc gatcgtgccg ggcgagcgga cggcaagata ggcaccgcac
     1261 cagccgcttt gcgtcatgag cctggcggcg tgcgcgaagc cgagcgccgc attgagcttg
     1321 aaacagggga tccgcggttc gctcacggcc agctcgcagc cgggaaactg cagcacgtcg
     1381 ccgatccaga ggtcgatctc ggtgacgccc tcgagcgtca ggttctcgcc gatcgaaccg
     1441 aaggccagcg gctcgtccca caatgccacc ttcgcctggg cgcggacggt ctgccagaac
     1501 gcgtagtgct cggaagggta ggcatagacg gccttgccga cgccgccgtg caccgacagg
     1561 tcggcctgct cgtcgccctc gatcccgagc gtgaagacac cgcgctcgcc gtcgacgggc
     1621 tgcttggcga tggccgtctt cacccggcgg ccgttgatcg acaccggtcg ggccagcgcc
     1681 gtgttcagac tgagcagccg gccggtcttc atcgactctt cttcgcggct ggccgatacc
     1741 cgcggtggcg ggagacgacg gcgcggccgc ggccgccgga gatccaggcc acgaagatct
     1801 tgccggcggg atgggcggcg cgaaaggaac gcatcgcgct caccgtttcg ccgagcatgg
     1861 ggtcggcctc gacgagcacg gcctcgggcg ggccgccgcc gaccgcccag gccccggcct
     1921 cgacgagggc gaacgcgttg cgggtccgca cctgggcgat gaacggcaat ttcgcatcgg
     1981 cagcgacgta ccagggtgcc gccgggtcga tgtgcgccgc ccgccagagc gcctgctcga
     2041 ccacatgcgt tccggagccg tcgccggcgg aaagaaagag catggttccc ggctccgccg
     2101 tcgccatctc ctggatccgg gccaacgcgt cgacgccgct ttggcccggc ggcggcggcc
     2161 ggccgcgcgt tcggggcagt ggcccgacga ggatgaattc gccagtcgcg atggtttgcc
     2221 ggtcgtagac gaggccctgc gcatcgagat cggtctcgac cgccggcacg ttggtcaagg
     2281 ccgcgtcgac ctcgccttcc ttcaccgcgt cgaggatggc cagcgccggt ccgggcgcga
     2341 gcttgacggc gaggccggtg tcggcgccga aggcgtgctg caacgcgtgc gccaggccgg
     2401 attcggccag ggcccgatcg acgccgagcc gcaacggcgt ctgggcggcg atcgccaggc
     2461 cggggttcgc cgtcacggcc gtggcggcca gcgaggcggc gagaagacgg cggcgatgca
     2521 tcgagccgat tattgcggcc tgcctcgtgc gatccccggt ccaggggcga tccggccgcc
     2581 gctacactgc ccgaagaaca aagacgcctt ttcagacggc tgtgccccgg gcgcaccgtt
     2641 tgcagggcga acgagacaac ctgccgcggc gcgcacgcgg tcgccgctcg cccggccgag
     2701 gcggccgccg cgggctgcgc ttttcattgc gattggacct gccatgaacc gtccccagcc
     2761 catcggcgcg accagccgtc cgcccgcttc cctgcgtcac cgaaagctgg ctgaatgggt
     2821 cgaggggatg gccgcgctga ccgagccatc cgacatcgtc tggtgcgatg gcagcgacgc
     2881 cgaatatgac cggctctgcg cgcgtctggt ggcctcgggg acgcttctca agctcaatcc
     2941 ggagctgcgt ccgcacagtt ttctggcgcg cagcagtgcc agcgacgtcg cccgggtcga
     3001 ggatcggacc ttcatctgct ccgagcgcga ggaggacgcc ggcccgacca accactggat
     3061 ggcaccgacc gagatgcgcg ccatccttcg cgacgggcgg ccagacggca cgccggcgct
     3121 cttcaagggg gcgatgcgcg ggcgcaccat gtatgtcgtg ccgttctcga tggggccgct
     3181 gggctccgac atcgcccata tcggcgtcga gctgaccgac agcgcctatg tcgccgtcag
     3241 catgaagatc atgacccgca tgggcgcgaa ggttctcgac gtcctgggca gcgacggcca
     3301 cttcgttccc tgcatgcaca ccgtcggggc gccgctcgag cccggccagg cagacagccc
     3361 ctggccgtgc aacccggtca agtacatcgt ccactacccc gagtcgcagg agatctggag
     3421 ctacggctcc ggctacggcg gcaacgcgct gctcggcaag aagtgcctgg cgcttcgcat
     3481 cgcctcggca atgggccggg ccgcggccga ttcggcgaag gccgagccgg gctggctggc
     3541 cgagcacatg ctggtcctgg gtgtcacttc gcccgaggga cgcaagcacc atgtcgccgc
     3601 cgccttcccg agtgcctgcg gcaagaccaa cttcgcgatg ctcattcccc cgccgtcgct
     3661 cgccggctgg aaagtgacga cgatcggcga cgacatcgcc tggatcaagc ccggcgccga
     3721 cggacgcctc ttcgccatca accccgaggc cggctacttc ggcgtcgctc cgggtaccaa
     3781 cgacaagacc aacccgaact gcatggcgag cctgaagcac gacgccatct tcaccaacgt
     3841 cgcgctcacc gatgacggcg atgtctggtg ggagggaatg accgagcagc cgccggcgca
     3901 cctgatcgac tggaagggcc aggactggac gccgcagatc ggtcgcgaca ccggcgccaa
     3961 ggcggcgcat ccgaatgccc gtttcaccgt cgccgcgacc aacaacccgg cgctcgatcc
     4021 ggcctgggat tcgccggcgg gtgttcccat cgatgctttc atcttcggcg gtcgccgctc
     4081 gacgacgatt cccctcgtca ccgaggcccg cgactggacc gagggcgtct acatggcatc
     4141 gaccatgggc tcggagacga ccgccgccgc ggccggcaag caaggcgtcg tgcgccgcga
     4201 tcccttcgcc atgctgcctt tcatcggtta caacatggcc gactacttcc agcactggct
     4261 cgacctcggc gccggtcttg cgaaacgcgg tgcgaccttg ccgaagatct tctgcgtcaa
     4321 ctggttcagg aagggcgagg acggcaagtt cgtctggccg ggctacggcg agaacatgcg
     4381 ggtcctcaag tggatcgtgc ggcggatcga gggcagtgcg ggagcggagc tcaccgcgtt
     4441 cggctggacg ccgcgctacg acgacatcga ctggtctggg ctcgatttcg accggcgccg
     4501 cttcgatcgg gtggccgacc tcgattcggc gagctggagt ggcgagctcg agctgcacga
     4561 cgagctgttc cgaaagctcg cgcacggctt gcccgaagcg ctgccgaagg tccgccggga
     4621 catcgcgcag cggctgcagc gctgagcgcg gcgagcgtgt cgatgcccga atcggcgccg
     4681 ccctcgcgcc cgccccccgg cgctgccgcg cacgatctca ccgaggcgga attcgccgag
     4741 ctcgacgggc tgcttgccgg catccccgag ccgctcgagc cgctcgacgt cgtgatgctc
     4801 gacggttttc ttgccggcgt cgtcgtccag ccgctcctgc tcgatgtcga gcagtggctg
     4861 ccttacgtct tcgattccgg cggccatcgc tggggcgaag ccgagccgtc gccagagcag
     4921 cgaagggcgc gcgagctcat cgtgcgccgc ctggcggcga tcaatcgatc gctggccgaa
     4981 taccgggctt tcgagccgct catcctcgag cccggcgatg ggtcggacga ggcagccgat
     5041 tcgcaaggcg aagaggcggc cgagccgggc ggcggtgcga cgatcgatgt cgactcgggc
     5101 gatggcgccg cttcgccggc cgccgaggcg caagacccgg ccgccgatcc ggtgaccgtg
     5161 gcgatcctgc cgtgggttgc cggattccag ttcgccgccg atctctttcc cggtctcgac
     5221 gaggtgccgg acgacggcgt cgcgacggcc ctggcgcggc tctatcgttt tctgccagcc
     5281 gaggaagacg acgaccgggc gacggtcgag ctggttcggc gcgagcgccc gctggcgtcg
     5341 atcgacgccg ccgtcgacga ggtggtcgcc tgcgtcgccg acctgtacga cctgaccgag
     5401 ccgctgcgct accgggtcga gacggttcgt cgcgccgagc cgaaagtggg acgcaacgac
     5461 ccgtgcccct gcggcagcgg gcgcaagttc aagcagtgcc acggcgccgg ttgaaagccg
     5521 cgcgatgcgg cttcaactgc tctccgacct ccatctcgag accgaggcct tcgatcccga
     5581 gccggcgccc ggggccgagt tgctcgttct tgccggcgac atcgacagcc gctgggacgg
     5641 cctggcgcgc tttgcgggat ggccgcaacc cgtgctcttc gtcgccggca accacgagtt
     5701 cgatggtcgt gatctcgacc tcggcatggc tgccttgcgg cgccgttgcg aggcggtcgg
     5761 catcacgatg ctcgaacgcg aaagcgtcgt cgtcgaggat gaccgcggcc ggccgatccg
     5821 cttcgtcggc accacgcggt ggagcgattt cgacctcttc ggcgagtccg ggcgggccaa
     5881 ggcgatgcgg gccggcgagt acttcatggc cttgatgaag acgagtcgcg acggtgcggc
     5941 gttcgatgcg gctgccgttc gcagcgaagc gctcatctgc cggaactggc tcgaagccga
     6001 gctggcgcgg ccgccaggca cctgggaacg aaccgtcgtc gtcacccact tcgcgcccag
     6061 cctgcgcagc gccgatcctc gctatggcga gcgaagcagc acggcgagct tctgcaacgc
     6121 cgacgacgac ctgctcggcc gtgccgacct ctggctgcac ggccacctgc a
//
LOCUS       DASRWS010000370         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_730053, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000370 DASRWS010000000
VERSION     DASRWS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730053"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VFF72_06980"
     CDS             complement(<1..1120)
                     /locus_tag="VFF72_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV92944.1"
                     /translation="MSNPKPSVDLLLRGVVVSMNAERDVWWDGFVAVTDGKIVDAGDA
                     SACQYEASEVVGGADTLIIPGLVNTHDHLVQGCMRGMAEGTTFQERLFGFYYPMTAAC
                     DEERSYWSALSPLVDLALSGVTTVADDHFTHLHKDSMDGVFRAIDTVGLRARVSRLVI
                     NEPGVVPENLRETIDTGLAETERLRKRWQSPLVNVTAGTVGITYVDPGDLHQLFDWTI
                     ANGEQFDIHVPSMMDKVYLEKRRGWSGGSFEWLDHEGMLGPNVIGIHAQRLAPGEHAL
                     VGERGASVSMVADMEALLGLVQFDAGSYLKHGATVSLGLDGPVVSYGQNLWHTMKSLM
                     IAQRLHDAADRLINGDRDWDNNLIFGSAEQALELATIGG"
     gene            1246..2118
                     /locus_tag="VFF72_06985"
     CDS             1246..2118
                     /locus_tag="VFF72_06985"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZV92945.1"
                     /translation="MSARSYLYVPGDRQDLLARAAGRGADALIVDLEDGVAASAKQAA
                     RGVVARWLESQATGAPVEIWVRVNPLSSGAGTVTAQDLKVAVAPGVTGICLAKCQGAA
                     EIEHFADLVDEAEARAGLKAGALRLSAILESAQGWLDAPHVAQARRVDRLQIGEADLL
                     AELGMTPGPDEAELVALRTQVVVASAAARIEAPIGPVSTNFRDPAGFRTTTTRLRRLG
                     FGGRATIHPAQVPIVNEIFTPSQAEVDSARAIIERFEAATSEGTGVIVAAGGDMVDEA
                     VVRAARRILGLVHP"
BASE COUNT          359 a          754 c          755 g          366 t
ORIGIN      
        1 cgccgccgat cgtcgcgagc tcgagcgcct gctcggcgct gccgaagatc aggttgttgt
       61 cccagtcgcg gtcgccgttg atcagccggt cggcggcatc gtgcagccgc tgcgcgatca
      121 tcaggctctt catggtgtgc cagaggttct ggccgtagga gaccaccggg ccgtcgaggc
      181 cgaggcttac cgtggcgcca tgcttcaggt aactgccggc gtcgaactgg acgagcccga
      241 gcagcgcctc catgtccgcc accatcgaca ccgaggcacc ccgctccccg acgagcgcgt
      301 gctcgcccgg cgccaggcgc tgggcgtgga tgccgatgac gttgggaccc agcattccct
      361 cgtggtcgag ccactcgaac gagccgccgc tccacccccg ccttttctcc aggtagacct
      421 tgtccatcat ggagggcacg tggatgtcga actgctcgcc gttggcgatg gtccagtcga
      481 agagctggtg caggtcgccc ggatcgacat aggtgatccc gaccgtgccg gctgtcacat
      541 tcaccagcgg tgactgccag cgcttgcgca gccgctcggt ctccgcgagc ccggtgtcga
      601 tggtttcccg caggttctca ggcacgaccc cgggctcgtt gatcacgagc cgggacaccc
      661 gggcgcgaag gccgacggtg tcgatggcgc ggaagacgcc atccatcgag tctttgtgca
      721 ggtgggtgaa gtgatcgtcg gccaccgtgg tgacgccgct caatgcgagg tcgaccagcg
      781 gcgagagcgc cgaccagtac gagcgctctt cgtcacacgc ggccgtcatc gggtagtaga
      841 acccgaacag ccgctcctgg aacgtggtgc cttcggccat gccccgcatg cagccctgca
      901 cgaggtggtc gtgggtgttg acgaggccgg gaatgatcag ggtgtcggcg ccgccgacaa
      961 cttcgctcgc ctcgtactgg cacgccgacg catccccggc gtcgacgatc ttgccgtcgg
     1021 tgacggcgac gaaaccatcc caccagacgt cgcgttctgc gttcatcgag acgacgactc
     1081 cgcgtagcaa cagatcaacc gagggtttcg ggttcgacat gaaggctccc gtctgtcccg
     1141 cttagcggaa cggcgtatcc gtgatatata cacccactca tcgcagaagg tcaacgaagc
     1201 cttgtagatt cggatcgaag cccccgtcgg caggagcgaa cctcgttgag cgcccgcagc
     1261 tatctgtacg tgcctggtga tcggcaggac ctgctcgccc gagcggccgg ccgaggcgcc
     1321 gatgcgctga tcgtcgacct cgaggacggc gtggccgcct cggcgaagca ggcagccaga
     1381 ggcgtggtcg ctcgctggct ggagagccag gcgactggcg cgccggtcga gatctgggtg
     1441 cgcgtcaacc cgctcagctc tggcgccggc acggtcaccg cacaggacct gaaggtcgct
     1501 gtcgcgcctg gcgtgaccgg gatctgcctg gccaagtgcc agggcgcagc ggagatcgag
     1561 cacttcgccg acctggtcga cgaggcggag gcgcgagccg ggctgaaggc cggtgccttg
     1621 cgcctgtcgg ccattctcga atcggcgcaa ggctggctgg acgccccgca cgtcgcgcag
     1681 gccaggcgag tcgaccggct ccagatcggc gaggccgacc tgctcgccga actcggcatg
     1741 acgccagggc cggacgaggc tgagctagtc gcgctcagaa cccaggtcgt cgtcgcgtcc
     1801 gcggccgcgc gaatcgaggc accgatcggc ccggtctcca cgaatttccg cgaccctgcg
     1861 gggttccgca ccacgacgac gcgcctgcgg cggctcggct tcggcggccg ggcgacgatc
     1921 catccggcgc aggtgccgat cgtgaacgag atcttcacgc catcgcaagc cgaggttgac
     1981 tcggcccgcg ccatcatcga gcgcttcgag gccgctacct cggagggaac gggcgtcatc
     2041 gtggccgccg gcggcgacat ggtggacgaa gccgtggttc gcgccgccag aagaattctt
     2101 ggcctggttc atccctagac tgacctcatg tcagtccagc ccgacggcga cgccgcgtca
     2161 tcatcaagtg tcaacgggat cgagcgtgcg ctcgaggtgc tgcggctctt cggtgagtcc
     2221 actgcgagca cgct
//
LOCUS       DASRWS010000371         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3890476, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000371 DASRWS010000000
VERSION     DASRWS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890476"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFF72_06990"
     CDS             <1..797
                     /locus_tag="VFF72_06990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZV92946.1"
                     /translation="SVFNLFFENSTRTRTTFEIAAKRLSADVINLDIARSSTAKGESL
                     LDTIANLSAMHADMFVVRHSESGAPYLIAQHCAPHVHVVNAGDGRHSHPTQGLLDMYT
                     IRHYKKSFHELTVAIVGDIVHSRVARSDIHALTTLGVPEIRAVGPKTLVPGDLKEMGV
                     RVCHDMAEGVRGADVIIMLRLQNERMGGAMLPSAGEFFKTFGLTADKLALAKGDAIVM
                     HPGPINRGVEIDSPVADGRQSVILPQVTFGIAVRMAVMSIIAGNSA"
     gene            803..2113
                     /locus_tag="VFF72_06995"
     CDS             803..2113
                     /locus_tag="VFF72_06995"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZV92947.1"
                     /translation="MDDRLLISNARLVDPATGRDEPGAVAVADGKVVGVGSAPAGFSA
                     DRTIDAAGAVVAPGLVDLCARLGEPGHEHEGLLEAELAAAVAGGVTSVVCPPDTEPVL
                     DEPGLVEMLKFRARNLDLARVFPLGALTRGLAGEAITEMVELTEAGCVGFGQAEVPIR
                     DTLVLHRALQYAATFGYTSWLRPSDAWLGQGIAAKGPLATRLGLAGVPVLAETIALAT
                     IFELVRDTGARVHLVRLSSAAGVELVRQAKAEGLPVSCDASIHQLHLIDVDIGYFDAA
                     ARLVPPLRQQRDRDAIRAGLADGSIDALVSDHTPVTEDAKHLPFAEAEPGATGLELLL
                     GLALKWGEAMRLDLPTTLAAVTSRPAALLATRQGAAVPAGLAVGAPADLCVFDAAAHW
                     QVEAERLRSRSRHTPFAGHALLGRVRWTIVGGRVCHEDRPSGER"
BASE COUNT          337 a          828 c          792 g          342 t
ORIGIN      
        1 agagcgtgtt caacctcttc ttcgagaact cgacgcggac gcggacgacg ttcgagatcg
       61 ccgccaagcg cctgtcggcc gacgtcatca acctcgacat cgcgcgctcg agcacggcca
      121 agggcgagag cctgctggac acgatcgcga atctttcggc gatgcacgcc gacatgttcg
      181 tcgtgcgcca cagcgagagc ggcgcgccgt atctcatcgc gcagcactgc gcgccgcacg
      241 tgcacgtggt caacgccggc gacggccgcc actcgcaccc gacgcagggc ctgctcgaca
      301 tgtacacgat ccgccactac aagaagagct tccacgagct caccgtggcg atcgtcggcg
      361 acatcgtcca ctcgcgcgtc gcccgctccg acatccatgc gctgacgacg ctcggcgttc
      421 ccgagatccg cgccgtcggc ccgaagacgc tcgtgccggg cgacctgaag gaaatgggcg
      481 ttcgcgtctg ccacgacatg gccgaaggcg tgcgcggcgc cgacgtcatc atcatgctgc
      541 ggttgcagaa cgagcgcatg ggcggcgcga tgctgccgag cgccggcgag ttcttcaaga
      601 cgttcgggct gaccgccgac aagctcgccc ttgccaaagg cgacgccatc gtcatgcacc
      661 ccgggccgat caaccgtggc gtcgagatcg attcgccggt ggccgacggc cggcaaagcg
      721 tcatcctgcc gcaggtgacc ttcggcatcg ccgtgcggat ggccgtcatg tccatcatcg
      781 ccggcaacag cgcctgatcc ggatggacga tcgcctgctc atctcgaatg cccggctcgt
      841 cgatccggcg acgggccgcg acgaacccgg cgccgtcgcc gtcgccgacg gcaaggtggt
      901 cggcgtcggt agcgcaccgg ccggcttctc tgccgaccga acgatcgacg cggccggcgc
      961 cgtcgtcgcg cccggcctcg tcgatctttg cgcccgcctg ggcgagcccg gccacgaaca
     1021 tgaaggcctg ctcgaagcgg agctcgcggc ggcggtcgcc ggcggcgtga ccagcgtggt
     1081 ctgtccgccg gacaccgagc cggttctcga cgagcccggc ctcgtcgaga tgctgaaatt
     1141 ccgcgctcgc aatctcgacc tcgcgcgagt ctttccgctc ggtgcattga cgcgcgggct
     1201 ggccggcgag gcgatcaccg agatggtcga gctcaccgag gccggttgcg tcggcttcgg
     1261 ccaggccgag gtgcccatcc gcgacacgct ggttctgcat cgcgcgctgc aatacgccgc
     1321 gaccttcggc tacacgagct ggctgcgccc gagcgacgcc tggctcggcc agggcatcgc
     1381 cgccaagggt ccgctggcga cgcgattggg gctggccggc gtgccggtgc tcgccgagac
     1441 gatcgccctc gcgacgatct tcgagctggt acgcgacacc ggggcccggg tccatctggt
     1501 ccggctgagc agcgccgccg gagtcgagct ggtgcgtcag gccaaggccg aggggctgcc
     1561 ggtcagctgc gacgcaagca tccaccagct gcatctgatc gacgtcgaca tcggctactt
     1621 cgacgccgcg gcccgtctcg tgccgccgct gcgtcagcag cgcgatcgcg acgcgatccg
     1681 cgccggcctc gccgacggca gcatcgatgc gctggtcagc gaccacacgc cggtgacgga
     1741 agacgcaaaa cacctgccgt tcgcggaggc cgagccgggc gccaccggac tggagctgct
     1801 gctcggcctg gcgctcaagt ggggcgaagc gatgcgcctc gacctgccga cgacgctcgc
     1861 cgcggtgacg agtcgcccgg cggcgttgct cgccacaagg caaggcgcag ccgtgccggc
     1921 ggggctcgcc gtcggcgcgc cggctgactt gtgcgtcttc gatgcggccg cccattggca
     1981 ggtcgaggcc gagcggctgc gcagccgcag ccggcacaca ccgttcgccg gccatgccct
     2041 gctcggacgg gttcgctgga cgatcgtcgg cggtcgtgtc tgccacgaag accggccgtc
     2101 cggcgagcgc tgagcgacgc ttcgccgcac gacgatgcgc cgacttcgcg ccgtcgcgcg
     2161 cctgctgcga agcgtcgcgc tcggcctgcg cggcctggcg atcgtcgtct tccggtttcc
     2221 atcgctgaca ccgctgcagc gacaggcgcg catccgctgg tgggcacagg gcatgctcga
     2281 ggcgatgggc gtggagttg
//
LOCUS       DASRWS010000372         5398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1297406, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000372 DASRWS010000000
VERSION     DASRWS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297406"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1083
                     /gene="glnL"
                     /locus_tag="VFF72_07000"
     CDS             13..1083
                     /gene="glnL"
                     /locus_tag="VFF72_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HZV92948.1"
                     /translation="MDLVAGRAALDMLATMVAIVTPEGECIFANSSFENVMGLSRRSM
                     SKSSMFDWFTDARQLRDTVVAVSGNDFATSRLDAQLKRPGASHGETLPVRVIVNQIDG
                     SRNVVFELVEIEQQTRQEREERALEQAEASKTLIRNLAHEIKNPLGGIRGSAQLLEMD
                     LESRALVEYTQVIIKEADRLQALVDRLLAPHRRPHVEGTVNIHEVCERVRALIAAEFP
                     RGLAIARDYDISIPEFRGDREQLIQAVLNIAHNAAQALAERVEAGDARITLRTRISRQ
                     VTLGKHRHRLALELHVEDNGPGVPEPLREKVFFPLVSGRESGTGLGLTLAQTFIQQHH
                     GIVEFESEPGRTVFKVLIPLLS"
     gene            1181..2659
                     /gene="ntrC"
                     /locus_tag="VFF72_07005"
     CDS             1181..2659
                     /gene="ntrC"
                     /locus_tag="VFF72_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HZV92949.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALEREEFAVRSFANPRDVLEALEDD
                     TPQLLVSDIRMPGGSGIELLNKVKARLPDLPVIIMTAYSDLDSAVSAFQGGAFDYLSK
                     PFDVPKAVDLIRRAVEESLRESSREVAGAEIPEMLGQAPAMQDVFRAIGRLSQSIVTV
                     LVTGESGSGKELVANALHKHSPRAGGPFVAINTAAIPKDLLESELFGHERGAFTGAQT
                     ARRGRFEQADGGTLFLDEIGDMPFDLQTRLLRVLSDGRFYRVGGHEPIRNNVRVVAAT
                     HQDLEARVKAGSFREDLFHRLNVIRLRLPPLRERREDIAGLTRFFLRKSARELGVEGK
                     RISDAALARLQQFEFPGNVRQLENICHWLTVMAPAQLVDVEDLPPELQGRSAGGAEAG
                     AAPTSPVSAPTQATTSASPPLPGSAAASPAWLDGLEEEAREMLRGGTPQVWETLTNRF
                     EAKLIETALDVTRGRRIEAASRLGIGRNTITRKIQELGIDEK"
     gene            complement(2704..3279)
                     /locus_tag="VFF72_07010"
     CDS             complement(2704..3279)
                     /locus_tag="VFF72_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HZV92950.1"
                     /translation="MSGHGFHAHGPHDHELEHQAEAEPHGMAGHLAVATAIIATIGAV
                     FSYESSVNQVNAGLYKNEAAIKKTEASNQWAYYQAKNNRQNMAEIAADLAPEARKAYY
                     RGEAARYKSEKTEIQKKAEELEKVSHEWDERSERELAGHERWQQATTFLQIAIALAAI
                     ALLARSKRMQIGVYGVTTVGVILGVMAWLGK"
     gene            complement(3282..3788)
                     /locus_tag="VFF72_07015"
     CDS             complement(3282..3788)
                     /locus_tag="VFF72_07015"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZV92951.1"
                     /translation="MPRSSPSVCLVAAVTRDGGIGLRGELLVRIPDDLRRLKRLTMGA
                     PIVLGRKTWQSIGRPLPGRRNIVVTRDPEWRAEGAEAAASLDSALSLAAGAPRIFVLG
                     GADIYALALPVADALELTEIDAAPPADTFFPSWNRAEFRQTAREEHATSEGLRYTFAS
                     YARIDQGK"
     gene            3848..>5398
                     /gene="scpA"
                     /locus_tag="VFF72_07020"
     CDS             3848..>5398
                     /gene="scpA"
                     /locus_tag="VFF72_07020"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HZV92952.1"
                     /translation="MTTSSDDDRAKALRTWQAAAARQAPNGDLGALAWTTPEGIVVKP
                     LYTAADLEQLPATDTLPGLPPYIRGPQATMYTVRPWTIRQYAGFSTAEASNDFYRKAL
                     AAGGQGISVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFAGIPLDKVSVS
                     MTMNGAVLPVLAGYIVAAEEQGVAMDELSGTIQNDILKEFMVRNTYIYPPAPSMRIVG
                     DIIAYTAERMPKFNSISISGYHIQEAGANQALELALTLADGREYVRTAIARGLDVDAF
                     AGRLSFFWAIGMNFYLEIAKMRAARGLWFRIMKEFAPKEARSSILRTHCQTSGWTLTA
                     QDPYNNVVRTTIEAMAAVFGGTQSLHTNSLDEAIALPTEFSARIARNTQLVIQEETHI
                     TSIVDPWAGSYAMETLTQQMADAAWTLIEEIESMSGMTKAVQSGWAKLKIEASAAEKQ
                     ARIDSGKDVIVGVNKYRLAEEEPLDTLTIDNHAVRESQIRRLEAVRANRDAAAVQTAL
                     AAMTAAARS"
BASE COUNT          918 a         1829 c         1812 g          839 t
ORIGIN      
        1 gccgcggcgc ggatcgacct agtcgccgga cgggcggcgc tcgacatgct ggcgacgatg
       61 gtcgccatcg tcacacccga gggcgaatgc atcttcgcga actcgtcgtt cgagaacgtg
      121 atgggcctgt cccggcgcag catgtcgaag agctcgatgt tcgactggtt caccgatgcc
      181 cggcaactgc gcgacacggt cgtcgcggtc agcggcaatg acttcgcgac gagccgcctc
      241 gacgcgcagc tgaagcgccc gggcgccagc cacggcgaga cgttgccggt gcgggtcatc
      301 gtcaaccaga tcgacggctc gcgcaacgtc gtcttcgagc tcgtcgagat cgagcagcag
      361 actcgccagg agcgcgagga gcgcgccctc gagcaggccg aggcctccaa gacattgatc
      421 aggaaccttg cccacgagat caagaatccg ctgggcggaa ttcgcggttc ggcgcagctg
      481 ctcgagatgg acctcgagtc gcgcgcgctg gtcgagtaca cgcaggtcat catcaaggag
      541 gccgaccgac tgcaggcgct tgtcgatcgc ctgctcgcgc cgcatcgccg gccccacgtc
      601 gaaggcaccg tcaacatcca cgaggtctgc gagcgcgtgc gcgcgctgat cgcggccgag
      661 tttccgcgcg gcctggcgat tgcccgcgac tacgacatct cgattcccga gtttcgcggc
      721 gatcgcgagc agctgatcca ggcggtgctc aacatcgccc acaacgcggc acaggcgctc
      781 gccgaacgcg tcgaggccgg cgatgcgcgg atcacgctgc gcacgcggat ctcgcgccag
      841 gtgaccctcg gcaagcaccg gcatcgactg gcactggaat tgcatgtcga ggacaacggt
      901 ccgggcgtgc ccgagccgtt gcgcgagaag gtcttctttc cgctggtttc gggtcgcgaa
      961 agcggcacgg gactggggct cactctcgcg caaacgttca tccagcagca tcatggaatc
     1021 gtcgaattcg aaagcgaacc gggccgcacc gtgttcaagg tcctgattcc tcttctttca
     1081 tgagcgcttc accgatgatg gcccggccgg gcgccgggcc gcttccaagt ccggccgcgc
     1141 tccttcggga agcagcgacc ggagggagcc tgggggcccg atgaaaccca tctggatcgt
     1201 cgacgacgat caatcgatcc gcttcgttct cgaaaaggcg ctcgagcgag aggaattcgc
     1261 ggtgcgcagc ttcgccaacc cgcgcgatgt gctggaggcg ctcgaggacg acaccccgca
     1321 gctgctcgtc tccgacatcc ggatgccggg cggctcgggc atcgagctgc tcaacaaggt
     1381 gaaggcgcgc ctgcccgacc tgcccgtcat catcatgacg gcctactccg acctcgacag
     1441 tgccgtgagc gccttccagg gcggcgcctt cgactatctg tccaagccgt tcgacgtgcc
     1501 gaaggccgtc gacctgatcc ggcgcgcggt cgaggaaagc ctgcgcgaaa gcagccggga
     1561 ggtggcgggc gcggagattc ccgagatgct cggccaggcg ccggcaatgc aggatgtctt
     1621 tcgcgccatc ggccggctga gccagagcat cgtcaccgtg ctggtcacgg gcgagtcggg
     1681 ctcgggaaag gaactcgtcg ccaacgcgct gcacaagcac agcccgcgcg ccggcggtcc
     1741 gttcgtcgcc atcaacaccg cggcgatccc caaggacttg ctcgagtcgg agctgttcgg
     1801 ccacgaacga ggtgccttca ccggcgccca gacggcgcgg cgcggccgct tcgagcaggc
     1861 cgacggcggc acgctgtttc tcgacgagat cggcgacatg ccgttcgacc tgcagacgcg
     1921 gctcctgcgc gttctttccg acggccgctt ctaccgtgtc ggcggccacg agccgatcag
     1981 gaacaatgtg cgcgtcgtcg ccgcgacgca ccaggatctg gaggcgcggg tcaaggccgg
     2041 atcgttccgg gaagacctgt tccaccggct caacgtgatc cggctgcgtc tgccgccgct
     2101 gcgcgaaagg cgcgaggaca tcgccggact gacccgcttc ttcctccgca aaagcgcccg
     2161 cgagctgggc gtcgagggca agcgaatcag cgacgccgcg ctggcgcggc tgcagcaatt
     2221 cgagtttcca ggcaacgtcc gccagctcga gaacatctgc cactggttga ccgtgatggc
     2281 gccggcccag ctcgtcgacg tcgaagattt gccccccgag ctgcaggggc ggagcgccgg
     2341 aggagctgag gctggcgctg cgccgacctc gcccgtgtcg gcgccgactc aggcgacgac
     2401 atccgccagc ccgccgttgc ccgggtcggc cgccgcgtca ccggcctggc tcgacgggct
     2461 cgaggaggag gcgcgcgaaa tgctgcgtgg tggcacgccg caggtctggg agacgctcac
     2521 gaatcgcttc gaggccaagc tcatcgagac ggcgctcgat gtcacgcggg ggcgccgcat
     2581 cgaggccgca agccgtctcg gcatcggccg caacacgatc acgcgcaaga tccaggagct
     2641 cggcatcgac gagaagtagt cgatgccgcg gccgcattcg aacgcggccg cgcgatcccc
     2701 gtgctatttc cccagccagg ccatcacgcc gaggatgacg ccgacggtgg tgacgccata
     2761 gacgccgatc tgcatgcgct tgctgcgcgc gagaagggcg atcgccgcca gcgcgatggc
     2821 gatctgcagg aaggtcgtcg cctgctgcca gcgctcgtgg ccggccagtt cacgttcact
     2881 cctctcgtcc cattcgtgcg aaaccttttc gagctcctcg gccttcttct ggatctcggt
     2941 cttctccgac ttgtagcgcg cggcctcgcc gcggtagtac gccttgcgcg cctcgggcgc
     3001 gaggtcggcg gcgatctcgg ccatgttctg ccggttgttc ttggcctggt agtaggccca
     3061 ctgattcgag gcctcggtct tcttgatcgc cgcctcgttc ttgtagaggc ccgcgttgac
     3121 ctggttcacg ctgctctcgt aggaaaagac ggcaccgatc gttgcgatga tcgccgtggc
     3181 cacggccaga tgcccggcca tgccgtgcgg ttcggcttcg gcctggtgct cgagttcgtg
     3241 gtcgtgcgga ccgtgcgcgt gaaagccgtg accggacatc tctacttccc ctgatcgatt
     3301 cttgcgtaac tggcgaacgt gtagcggagg ccttcggacg tcgcgtgttc ctcgcgcgca
     3361 gtctgcctga attcggcccg gttccaggac gggaagaagg tgtcggccgg cggcgcggcg
     3421 tcgatctcgg tgagctcgag cgcatcggcg accggcagcg ccagggcata gatgtcggcg
     3481 ccgccgagga cgaagatgcg cggcgcgccg gcggccagcg acaatgccga atcgagagac
     3541 gcggccgcct cggccccttc ggcgcgccat tcggggtcgc gggtgacgac gatgtttcgc
     3601 cgtcccggca gcggcctgcc gatcgactgc caggtcttgc ggcccagaac aatgggcgcg
     3661 cccatcgtca gtcgcttgag gcgacgcagg tcgtcgggga tgcggaccag caactcgccg
     3721 cgcaggccga tgccgccgtc gcgggtgacg gcggcgacaa ggcagacgga aggggaagac
     3781 cgcggcatgg gtcggggatt gtcgcctgcc tcgccgcgat aatcggcggg cttccctccg
     3841 aagagccatg acgacttcat ccgacgacga ccgcgcgaag gccctgcgca cctggcaggc
     3901 ggccgccgcg cggcaggccc cgaatggcga cctcggcgca ctggcctgga cgacgcccga
     3961 aggcatcgtc gtcaagccgc tatacaccgc cgccgacctc gagcaactgc ccgccacgga
     4021 caccttgccc ggcctgccgc cctacatccg cggcccgcag gcgacgatgt acacggtgcg
     4081 gccgtggacc atccgccagt acgccggctt ttccaccgcc gaggcatcga acgacttcta
     4141 tcgcaaggcg ctggccgccg gcggccaggg catcagcgtc gccttcgacc tggcgacgca
     4201 tcgcggctac gacagcgacc atccgcgcgt caccggcgac gtcggcaagg ccggcgtggc
     4261 gatcgattcg gtcgaggaca tgaagatcct cttcgccggc atcccgctcg acaaggtgag
     4321 cgtctccatg accatgaacg gcgcggtgct gccggtgctg gccggctaca tcgtcgccgc
     4381 cgaggagcag ggcgtcgcca tggatgagct ctcaggaacg atccagaacg acatcctgaa
     4441 ggagttcatg gtccgcaaca cctacatcta tccgccggcc cccagcatgc gcatcgtcgg
     4501 cgacatcatc gcctacacgg ccgagcgcat gccgaagttc aactcgatct cgatctcggg
     4561 ctatcacatc caggaagccg gcgcgaacca ggcgctcgag ctggcgctga ccctggccga
     4621 cggtcgcgaa tacgtgcgca cggcgatcgc gcgcggcctc gacgtcgacg ccttcgccgg
     4681 tcgactcagc ttcttctggg cgatcgggat gaacttctat ctcgagattg ccaagatgcg
     4741 cgcggcgcgc gggctctggt tcaggatcat gaaggagttc gccccgaagg aggccaggag
     4801 ctcgatcctg cgtacccact gccagacgtc gggctggacg ctgacggcgc aggatcccta
     4861 caacaacgtc gtgcgcacga cgatcgaggc gatggccgcg gtcttcggcg gcacccagag
     4921 cctgcacacg aactcgctcg acgaggcgat cgcgctgccc acggaattca gcgcccgcat
     4981 cgcccgcaac acccagctcg tgatccagga ggagacgcac atcacgtcga tcgtcgatcc
     5041 ctgggcaggc agctacgcca tggagacgtt gacgcagcag atggccgacg ccgcctggac
     5101 gctgatcgag gaaatcgaat cgatgagcgg gatgacgaag gccgtgcaga gcggctgggc
     5161 gaagctgaag atcgaggcaa gcgctgccga gaagcaggcg cgcatcgatt cgggaaagga
     5221 cgtgatcgtg ggcgtcaaca agtaccgcct cgccgaggag gagcctctcg atacgctcac
     5281 catcgacaac cacgcagtgc gcgagagcca gatccggcga ctcgaagcgg tccgcgcgaa
     5341 tcgcgacgcg gccgcggtgc agacggcgct ggcggcgatg acggcggccg cgcggtcg
//
LOCUS       DASRWS010000373         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2433939, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000373 DASRWS010000000
VERSION     DASRWS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433939"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="fmt"
                     /locus_tag="VFF72_07025"
     CDS             complement(<1..698)
                     /gene="fmt"
                     /locus_tag="VFF72_07025"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZV92953.1"
                     /translation="MRLAFAGTPEFARVALASLCEAGHEVVAVLSQPDRPAGRGLRLQ
                     ASPVKDFATRAALPVLQPRGLRLDGRFAEDAAAAQSALVGAAPEAIVVAAYGLILPGW
                     LLTLPPRGCINIHASLLPRWRGAAPIQRAIEAGDAETGITLMQMDAGLDTGDILMTAS
                     TPIGVDDTGASLQARLARLGAGLAVQGLVAMAGGHLGGRRQPEDGVTYAHKIVKAEAV
                     IDWREPAAAIERRLR"
     gene            complement(721..1233)
                     /gene="def"
                     /locus_tag="VFF72_07030"
     CDS             complement(721..1233)
                     /gene="def"
                     /locus_tag="VFF72_07030"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZV92954.1"
                     /translation="MARLDILRYPDPRLHKVARRVDEVDARIRTLVDDMLETMYGAEG
                     VGLAATQVDVHERVIVMDTSEARDSGIVLINPELVQASDEMILGEEGCLSVPEIYDKV
                     TRHARVRVRAADRSGEVREFDAEGLLAVAVQHEMDHLLGKVFVEYLSPLKRNRIRARM
                     LKKTRDEERP"
     gene            1516..>2203
                     /locus_tag="VFF72_07035"
     CDS             1516..>2203
                     /locus_tag="VFF72_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZV92955.1"
                     /translation="MSRRSLFAPSTRSRLTPRLAAGLAALGLTAVALAATPRYPVTEA
                     QRATARRTAEAGVPLSEIKADAPDVYTVKHHDTLWGISGMYLKSPWRWPELWGMNLDE
                     VKNPNLIYPGQTLYLDKSNGRARLRMGEMAGGPVGETRLSPRVRATDIALQGISSIPF
                     NLIEPFLNEAVIFEGNELASAPRIVATQEGRVLLSRGDTAYVRGDVSNASEFRIFRQA
                     RPLHDPLTKEI"
BASE COUNT          347 a          768 c          748 g          340 t
ORIGIN      
        1 cgcagccgcc tttcgatcgc cgccgccggc tcgcgccagt cgatcaccgc ctcggccttg
       61 acgatcttgt gcgcatacgt cacgccgtcc tccggctgcc gtcggccgcc gaggtggccc
      121 ccggccatcg caaccagccc ctgcacggcc agaccggccc cgagccgcgc caggcgcgcc
      181 tgcaggctgg cgccggtgtc gtcgacgccg atcggcgtcg acgcggtcat gaggatgtcg
      241 ccggtgtcga ggccggcgtc catctgcatc aaggtgatgc cggtttcggc gtcgccggcc
      301 tcgatggcgc gctggatcgg cgcggcgccg cgccagcgcg gcagcagcga tgcatggatg
      361 ttgatgcagc cgcgcggcgg cagcgtgagg agccagccgg gcaggatgag gccgtaggcg
      421 gcgacgacga tcgcttcggg cgccgccccg acgagggcgc tttgcgcggc cgcggcatcc
      481 tcggcgaagc ggccatcgag gcgcaggccg cgaggctgga gcaccggcaa cgccgcccgc
      541 gttgcgaaat ccttgaccgg cgaagcttgc agcctgaggc cccggccggc cggccgatcg
      601 ggctggctga ggaccgcgac gacttcgtgc ccggcttcgc acaggctcgc cagggcgacg
      661 cgggcgaact ccggcgtgcc cgcgaaggcg agcctcatct gccgccgtcc ggaaggacgc
      721 tcagggtcgc tcctcgtcgc gcgtcttctt gagcattcgt gcgcggatgc ggttgcgctt
      781 gagcgggctc aggtattcga cgaacacctt gcccaggaga tgatccatct cgtgctgcac
      841 ggcgacggcg aggagaccct cggcgtcgaa ctcgcgcact tcgcccgacc ggtcggcagc
      901 gcgcacccga acccgggcgt ggcgcgtcac cttgtcgtag atctccggca ccgacaggca
      961 gccttcttca ccgaggatca tctcgtcgct ggcctggacg agctcgggat tgatcaacac
     1021 gatgccgctg tcgcgcgcct ccgaggtgtc catgacgatg acccgctcgt gcacgtcgac
     1081 ctgggtcgcg gccaggccga cgccctcggc gccgtacatc gtctcgagca tgtcgtcgac
     1141 gagggtgcgg attcgcgcat cgacctcgtc gacacgccgg gcgaccttgt gcaaacgggg
     1201 atcggggtaa cgaaggatgt cgagacgggc catttgtgac gattcgattc aggcgtcggg
     1261 cgctgtggac gacaagcaac gtaacgtggt gtgaaaggtc tcacgcacgc cgtctgcagc
     1321 gggctacttt cgttgcgata tcaaggcttt atgggcagaa tctgccggac agagtgaaga
     1381 ttttcgccga accgcgccga ctgcggagag gccgggcgaa gtgacctcgc aatcggattc
     1441 cgattcattg cgccggccgc agagctgcaa gaaaccatcg cgggcaaggg tgcccacgca
     1501 atgagacccg cctccatgag ccgtcgatcg cttttcgctc cgtctacccg cagccgcctg
     1561 acgccccgcc tcgcggccgg tctcgcggcg ctcggcctca cggctgtggc gctggcggcg
     1621 accccccggt atccggtgac cgaggcgcaa cgggccacgg ccaggcgcac cgccgaagcc
     1681 ggtgtcccgc tcagcgagat caaggccgac gctcccgacg tctacaccgt caagcaccac
     1741 gacaccttgt ggggcatctc gggcatgtac ctgaagagcc cctggcgctg gcccgagctc
     1801 tggggcatga acctcgacga ggtgaagaac cccaacctca tctatccggg gcagacgctc
     1861 tatctcgaca agtcgaacgg ccgcgcgcgt ttgcgcatgg gcgagatggc aggcgggccg
     1921 gtcggtgaaa cccggctgtc gccgcgcgtt cgcgccaccg acatcgcgct ccagggcatc
     1981 tcctcgattc cgttcaacct gatcgagccg ttcctgaacg aagcggtgat cttcgagggc
     2041 aacgagctcg cgagcgcgcc gcgcatcgtc gccacgcagg aaggccgcgt gctgctgtcg
     2101 cgcggcgaca ccgcctacgt gcgcggcgac gtgagcaatg cgagcgagtt ccgcatcttc
     2161 cgccaggccc gaccgctgca cgacccgttg acgaaggaga tcc
//
LOCUS       DASRWS010000374         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1297675, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000374 DASRWS010000000
VERSION     DASRWS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297675"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VFF72_07040"
     CDS             complement(<1..1226)
                     /locus_tag="VFF72_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZV92956.1"
                     /translation="MKNTHLVPVAVVLLALGRGFPAAAQSVSLPAAPASAASATTLDP
                     VVVTATRSPRALSTVPASVSVIGAEQIEDTPALALDDVLRHVAGVDLPIQSGIQAHPT
                     ADNVSMRGLGGIRALVLVDGVPINDPFFGTVQWARVPLENIDRVEIVRGGGSPLWGND
                     AMGGAIHIITRDPTVDTAVVDVGGGSYDTYRTNLFASYGLGASHRLSLDANLDGTRGF
                     QAVPLYARRPFDTPTSFSARNIQLSDRFTPSPDLSADFSLTHHENDQRLGTFLNTNTQ
                     STTNYVARLRRTFGTDTSLTGTLFHGDSRFVTRNPTVTDPSLPIDTQTEHLDNVHTTP
                     VHDTGGSLVLSRDMATMLKNITLGVDFHRIGGSDSAAIYDATGNGQVRTDYGSGKEQF
                     IGGFVQGSLKPVDRLE"
     gene            complement(1250..2608)
                     /locus_tag="VFF72_07045"
     CDS             complement(1250..2608)
                     /locus_tag="VFF72_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZV92957.1"
                     /translation="MSNVTGQALPAQASALYRRIWRWHFYAGLVCLPVIVLLATTGAL
                     YLFRVEIEAVVYRHELENAGHGAATLAASEAIRRAEATGGAPIAYSPPARPGASAMVL
                     MQHADGERRQVFVDTSTGTVLGSIAERYRLMPLLRSLHSLSFFGKAAGFIVEIVAGWV
                     IVLVTTGVYLWWPRRQSGGVVTVRQTPRQRIWWRDLHAVTGAFAAVVIAFLALTGMPW
                     SAFWGERFNQAMTDAGLGVPAAFWNEVPQSEAPLSSLGSVPWTVHEHPMPLSATPPAA
                     HAAGGIDVDQAVSRLAALGLTGGYQLNLPIGPRGVYSAVYLPDHVRDQRVVHLDRYSG
                     RVLADIDYSHYGAVGKVTEWGVSVHMGREFGRANQLVLLAGCLALLAVAASGVVMWWK
                     RRPRGLLAAPPRRDADRIGRNVAVMAIVLGAVFPLLGLSMLVALVIDVLVPSLWRGHW
                     AL"
BASE COUNT          447 a          852 c          952 g          396 t
ORIGIN      
        1 aattcgagcc ggtcgaccgg cttgagcgag ccctgcacga acccgccgat gaactgttcc
       61 ttgccgctgc cgtagtcggt ccggacctgg ccgttgccgg tcgcgtcgta gatggcggcg
      121 ctgtcggagc cgccgatgcg atgaaagtcg acgcccagcg tgatgttctt cagcatcgtg
      181 gccatgtcgc gagacaggac aagcgagccg ccggtgtcgt ggacaggcgt cgtgtgcacg
      241 ttgtcgagat gctccgtctg cgtgtcgatc ggcagggacg gatcggtcac ggtcgggttg
      301 cgggtgacga acctgctgtc gccatgaaag agtgtcccgg tcaaggacgt gtccgtaccg
      361 aacgtcctgc gaaggcgcgc gacgtagttc gtggtgctct gcgtgttggt gttgaggaag
      421 gtgcccagcc gctggtcgtt ctcgtggtgc gtcaggctga aatcagccga gaggtctggc
      481 gaaggcgtga agcggtcgct cagctggatg tttcgcgccg agaagctcgt cggcgtgtcg
      541 aagggacggc gcgcatacag cggcacggcc tggaacccgc gcgtcccgtc gaggttcgcg
      601 tccaggctca ggcgatggct tgcgccgagc ccgtacgaag cgaacaggtt cgtgcgatag
      661 gtgtcgtagc tgccgccgcc gacgtcgacg acggccgtgt cgacggtcgg atcacgggtg
      721 atgatgtgga tcgccccgcc catggcgtcg ttgccccaga gcgggctacc gccgccgcga
      781 acgatctcga ctcgatcgat gttctcgagg ggaacgcgtg cccactggac ggtgccgaag
      841 aaggggtcgt tgatcggcac gccatcgacc aggaccagtg cgcggatgcc gccgaggccg
      901 cgcatcgaga cgttgtcggc cgtcgggtgc gcctggattc cgctctggat cggcaggtcg
      961 acgccggcca cgtggcgcag cacgtcgtcg agcgccagcg cgggggtgtc ctcgatctgc
     1021 tccgcgccga ttaccgacac gctggccgga acggtgctca acgcccgcgg cgaacgcgtc
     1081 gccgtgacga cgacgggatc gagcgtggtt gcgctggcgg ccgaagccgg tgccgccgga
     1141 agcgagacgg actgcgctgc cgccgggaat ccgcgtccca gcgccagaag cacgaccgcg
     1201 accggtacaa ggtgggtgtt cttcacgttc tgctctttga aggatggcgt cacaacgccc
     1261 agtggccacg ccacagtgag ggcacgagaa cgtcgatgac gagcgcgacc agcatcgaca
     1321 gtccgagcag cgggaagacg gcaccgagca cgatcgccat caccgcgacg ttgcggccga
     1381 tgcgatcggc atcgcgtcgg ggtggcgccg caagaaggcc gcgtggccgg cgcttccacc
     1441 acatgacgac gccgctggcg gcgacggcaa ggagggcgag gcagcccgcg agcagcacca
     1501 gctggttggc gcgcccgaac tcgcgcccca tgtgcacgct gacaccccat tcggtcacct
     1561 tgccgacggc gccgtagtgc gaatagtcga tgtcggcgag aacgcggccg ctgtagcgat
     1621 cgagatggac gacgcgctgg tcgcggacgt gatcgggtag atagaccgcg ctgtagacgc
     1681 cgcgcggtcc gatcggcaga ttcagctgat atccgccggt gagcccgagg gctgccagtc
     1741 gcgagacggc ctggtcgaca tcgatgccgc ctgcggcgtg cgcggccggc ggtgtcgccg
     1801 acagcggcat ggggtgctcg tgcactgtcc aaggcacgct ccccaggctc gagagcggcg
     1861 cctccgactg cggaacctcg ttccagaatg ccgccggtac gccgagcccg gcgtcggtca
     1921 tcgcctggtt gaagcgctcg ccccagaacg ccgaccaggg catcccggtg agggcgagga
     1981 acgcgatcac gacagcggca aaggcaccgg tgaccgcgtg caggtcccgc caccagatcc
     2041 gttgccgcgg cgtctgccgc acggtgacga caccgcctga ctggcggcgc ggccaccaca
     2101 ggtagacgcc ggtcgtgacg aggacgatga cccagccggc gacgatctcg acgatgaagc
     2161 ccgccgcctt gccgaagaag ctgagcgaat gcagcgaccg caatagcggc atcaaccggt
     2221 agcgttcggc gatcgatccg agcacggtgc cggtcgatgt gtcgacgaag acctgccgcc
     2281 gctcgccgtc ggcgtgctgc atcagcacca tggcgctggc gcccggacgt gccggcggcg
     2341 agtacgcgat cggcgcaccg cccgtcgcct cggcgcgccg gatcgcttcg ctcgcagcga
     2401 gcgtggcagc gccatggcct gcgttctcga gctcatgccg gtagaccacg gcctcgatct
     2461 cgacacgaaa aagatacagc gcaccggtcg tcgcgagcag gacgatgacc ggcaggcaga
     2521 cgagcccggc gtagaagtgc cagcgccaga tacggcggta gagggcggag gcttgagccg
     2581 gcagagcctg gccggtgacg ttcgacatcg agacaacttc ccgttggagt ctgcaagcgc
     2641 gcggtcg
//
LOCUS       DASRWS010000375         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1865530, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000375 DASRWS010000000
VERSION     DASRWS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865530"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2211
                     /locus_tag="VFF72_07050"
     CDS             <1..>2211
                     /locus_tag="VFF72_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZV92958.1"
                     /translation="TAQHARTIFHGDFEQASDALQAALAGDGSTWPQIRQLVATVVDI
                     AHALIDETRHTSGGASDEARFWAGALELAARSHCRDDACAPGQESPAARLNALADVAL
                     QMADAMQFGFLLDPVRGLLSIGYAVDEGRLDPSSYDLLASEARLASFVAIAKGDVPAR
                     HWFRLGRVATPVPGGAALVSWSGSMFEYLMPSLVMRAPAESLIVRTNSLVVRRQIAYG
                     ETLGLPWGTSESAFNARDLEFTYQYSSFGVPDLGLKRGLGYSIVIAPYATALAAMIAP
                     AAAMRNFEQLGAAGASGRFGFYEALDYTSARMPQGQRVAVVRAFMAHHQGMSIVAIAN
                     ALLGGRLRAFFHTEPSIQATELLLQERMPGSVAAAHLRINEDHIEGVMRGARPAVARR
                     LLSATGASPATHLLSNGRYAVMLTAAGSGYSHRGDLAVTHWSEDATCDDSGSYLYLRD
                     AESGELWSATWQPTGVVPHRYEVNFMEDRAEFLRRDGTLVTRLDVIVSPEEDAEVRRV
                     SVTNDGARVRVIEFTSYAQIVLAPPAAYQAHPAFSKMFVETEFLPASNAILATRRRRE
                     VGEPEVWAAHLARISGDAVGAVEIETDRARFIGRGKSVRCPQGLASGRPLGNSSGVVL
                     DPAFVLRQRLRIKPGETASIDFWTIVADTRAQLLDLVDKHRDGEPFARAAMLAWTQAQ
                     IQLRYNGVSPDEACLFQQLAGYLLYNNASLRPVSAALLRGAAAAAAAGGVGAAPL"
BASE COUNT          334 a          727 c          761 g          389 t
ORIGIN      
        1 tacggcgcag catgcccgga cgattttcca cggcgatttc gagcaggcat ccgatgcgct
       61 gcaggccgcg cttgccggcg acggatcgac atggccccag atccggcagc tcgttgcgac
      121 ggtagtcgac attgcacacg cgctgatcga tgaaacccgc catacgtcgg gcggtgcgag
      181 cgacgaggcg cggttctggg ccggcgcgct cgagctggcc gcgcgcagcc actgccgcga
      241 tgatgcctgc gctccagggc aggaatcgcc ggcggctcgg ctcaacgcgt tggctgatgt
      301 cgcgctgcaa atggccgatg cgatgcaatt cggctttctg ctggatccgg ttcgcggcct
      361 gctgtcgata ggctacgccg tcgacgaagg ccggctcgac ccgagcagtt acgacctgct
      421 ggcctccgag gcgcggctgg ccagtttcgt cgccatcgcc aagggcgacg ttcccgcgcg
      481 ccactggttc cggctgggtc gcgtcgccac gccggtgcca ggcggcgcag cactggtttc
      541 ctggtcaggc tcgatgttcg aatacctgat gccgtcgctg gtgatgcgcg cgccggccga
      601 aagcctgatc gtgcgcacca acagtcttgt cgtgcgtcgg cagatcgcct acggcgagac
      661 gctcggcttg ccctggggca cttccgaatc cgctttcaac gcgcgggatc tcgaatttac
      721 ctatcaatac tcgagcttcg gcgttccgga cctggggctc aagcgcggtc ttggctatag
      781 catcgtgatc gcaccgtatg cgacggccct ggccgcgatg atcgcgccgg ccgcggccat
      841 gcgcaacttc gagcagcttg gcgcagccgg cgccagcggc cggttcggtt tctacgaggc
      901 actggactac acgtccgcgc gcatgcccca gggtcagcgg gtcgcggtgg tgcgcgcctt
      961 catggcgcat caccaaggca tgtcgatcgt tgcgattgcc aatgcgctgc tgggcggacg
     1021 cctgcgcgcg ttctttcaca ccgagccgtc gatccaggca accgaactgc tgctgcagga
     1081 acgcatgccg ggcagtgtgg cggccgcaca cctgcgcatc aacgaggatc acatcgaggg
     1141 tgtgatgcgc ggcgccaggc ccgcggtggc acggcgcctg ctgtcggcga ctggcgcatc
     1201 gcccgcgacc cacctgcttt ccaacggccg ctacgcagtg atgctgacgg cggccggctc
     1261 ggggtacagc caccgcggcg atctggccgt cacgcactgg agcgaggacg ccacgtgcga
     1321 cgactcgggc agctatctgt atctgcgcga cgccgagagc ggcgaactct ggtcggcgac
     1381 gtggcagccg accggagtcg tcccgcatcg ctatgaagtg aacttcatgg aggatcgtgc
     1441 ggagttcctc cggcgcgatg gcacgttggt gacccggctc gatgtaatcg tatcgcccga
     1501 ggaagacgcg gaagtgcgac gggtgtcggt gaccaacgac ggcgcgcgcg tgcgcgtcat
     1561 cgagttcacg tcttatgcgc agatcgttct tgccccgccg gccgcctatc aggcgcatcc
     1621 ggcattctcg aaaatgttcg tcgaaaccga gttcctgccg gcgtccaacg ccattttggc
     1681 gacacgccgt cggcgcgaag tgggcgagcc cgaggtctgg gccgcgcatc ttgcgcggat
     1741 ttccggcgat gccgtcggag ccgtcgaaat cgaaaccgac cgggcccggt tcatcggacg
     1801 cgggaaaagc gtgcgttgcc cgcagggcct tgcgagcggg cggccgctgg gcaattcgtc
     1861 gggtgtggtg ctcgaccccg ccttcgtgct gcgccagcgc ctgcgcatca agccgggcga
     1921 gacggccagc attgatttct ggaccatcgt cgccgatacc cgtgctcaat tgctggacct
     1981 ggtggacaag catcgcgatg gcgagccgtt tgcacgggcg gcgatgctgg cctggaccca
     2041 ggcccagata cagctgcgct acaacggcgt gagtcccgat gaagcctgcc tgttccagca
     2101 gttggcgggc tatctgctgt acaacaatgc gtcgcttcgg ccggtttcgg ccgcgctgct
     2161 gcgcggcgcg gcggcggcgg cggccgcagg cggcgtgggc gccgcgccgc t
//
LOCUS       DASRWS010000376         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2728354, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000376 DASRWS010000000
VERSION     DASRWS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728354"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..786
                     /gene="solA"
                     /locus_tag="VFF72_07055"
     CDS             4..786
                     /gene="solA"
                     /locus_tag="VFF72_07055"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HZV92959.1"
                     /translation="MLDAASVMRRFPAFRLPSDFIGVLQPEAGILAPEATVHAQIALA
                     RAAGADVRTNERVHAIEPGPAGVRIVADSAVIEARQVVVAAGAWLPRLLPGLPVPLRV
                     TRQAVGWFAPSDPAVFARERCPIFMIECAEGIFYGFPRGPEPGIKFAKHHHADEAVDP
                     ARPTRPVDGSDETLLRAALAAHVPSANGDLLASQTCLYTMTPDGDFILDRLPGAPAII
                     VASPCSGHGFKFAPVIGEILADLVTNGATRHDISRFRLARFG"
     gene            904..>2469
                     /locus_tag="VFF72_07060"
     CDS             904..>2469
                     /locus_tag="VFF72_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZV92960.1"
                     /translation="MLGQMQDWPLLIHRIIDHAATYHGGRPVISRSIEGPFHTTTYAE
                     IRARAKKVAQRLARDGIKPGDRVATLAWNTWRHLESWYGIVGIGGVYHTVNPRLFPDQ
                     IAWIVNHAEDRILMTDVTFVPLLEKLAGRLPTIERYIVYADAAHMPATSLKNAVSYEE
                     WLAEADGDIAWKELDENAAAGMCYTSGTTGNPKGVVYSHRSNVLHAMAHSGAVFSMSS
                     RDVILPVVPLFHANSWSLAFSCPLAGASLVMPGMKLDGPSVYELLDTCKVTCTAAVPT
                     VWLMLLQHLEKTGDKLPHLKRVIIGGSACPRAMTKTFQDTYGVEVIHAWGMTEMSPLG
                     SACTLIPEYAELTGDARLDIQMKQGQPPFSIEMKITDDEGRELPWDGTTFGRLKVRGP
                     AIAKSYFKGEGGDILDENGFFDTGDVATMDPHGYMQITDRAKDVIKSGGEWISSIDLE
                     NLAVGHPSVAEAAVIGIRHPKWDERPLLVVVLKSGQKATRDDILGFMVGKIAHWWMPD
                     DVVFVDEIPHTATG"
BASE COUNT          424 a          859 c          779 g          407 t
ORIGIN      
        1 gaggtgctcg acgcggccag cgtgatgcgg cggtttccgg cgtttcggct gccgtcggac
       61 ttcatcggcg tgctgcagcc ggaggccgga attctcgcac ccgaagccac cgtccacgcc
      121 cagatcgccc ttgcgcgtgc ggccggtgcc gacgtgcgca cgaacgagcg cgtgcacgcg
      181 atcgagccag ggccggccgg cgtgcgcatc gtcgccgata gcgctgtcat cgaggccagg
      241 caggtcgtcg tcgcggccgg cgcgtggctt ccgcgacttc tgcccggcct tcccgttccg
      301 ctgcgcgtga cccgccaggc ggttgggtgg ttcgcaccat ccgatcccgc cgtcttcgcg
      361 cgcgagcgct gcccgatctt catgatcgaa tgcgccgaag gcatcttcta cggctttccg
      421 cgcggacctg agccaggcat caaattcgcc aagcaccatc atgccgacga agccgtcgat
      481 cctgcgaggc ccacgcgacc ggtggacggc tccgacgaaa ccctgctacg cgcggccctc
      541 gcggcgcacg tgccgtcggc caatggcgac ctgctcgcct cacaaacctg cctctacacc
      601 atgacgccgg acggcgattt catcctcgac cgcctgcccg gcgcgccggc gatcatcgtt
      661 gcctccccgt gctccggcca cggcttcaag ttcgccccgg tgatcggcga aatcctcgcc
      721 gacctcgtca caaacggtgc gacccggcac gatatctcac ggtttcgcct agcgcggttc
      781 ggttgaaccc gaattgccgc aggcccgtag ctatgggaac tgttgcacgg cgcgggcgga
      841 acgctaacct tggggccagg tcgcgaactc aagaccataa ggcggcgact cggaggaagc
      901 ggaatgctcg gccagatgca ggattggccc ctgctcatcc atcgcatcat cgatcacgcg
      961 gccacctatc atggcggccg gcccgtcatt tcccgctcga tcgaaggccc gtttcacacg
     1021 acgacctacg ccgagatccg agcgcgggcc aagaaggtcg cgcagcgcct cgcgcgcgac
     1081 ggcatcaagc ccggcgaccg ggtcgcgacg ctcgcgtgga atacgtggcg gcacctcgaa
     1141 tcctggtacg gcatcgtcgg catcggcggc gtctatcaca ccgtcaaccc gcgcctgttt
     1201 cccgatcaga tcgcctggat cgtcaaccac gcggaagacc gcatcctgat gacggatgtc
     1261 accttcgtgc cgctgctgga gaagcttgcc ggcaggctgc cgacgatcga gcgttacatc
     1321 gtctacgccg acgccgctca catgccggcg acctcgctca agaatgcggt ctcctacgag
     1381 gaatggctcg ccgaggcgga tggcgacatc gcctggaagg agctcgacga gaacgccgcg
     1441 gccggcatgt gctacacgtc gggcaccacc gggaacccga agggcgtcgt ctactcgcac
     1501 cgctccaacg tgctgcacgc gatggcgcat tcgggggccg tgttctcgat gtcgtcacgc
     1561 gacgtgatcc tgccggtggt gccgctcttt catgccaata gctggtcgct cgccttttcg
     1621 tgcccgctcg ccggcgcgag cctggtgatg cccggcatga agctcgacgg cccttcggtc
     1681 tacgaattgc tcgacacctg caaggtcacc tgcacggccg cggttccgac cgtctggctc
     1741 atgctgctcc aacacctcga gaagaccggc gacaagctgc cccatctcaa gcgcgtcatc
     1801 atcggcggct ccgcctgccc gcgcgccatg accaagacgt tccaggacac ctacggggtc
     1861 gaggtgatcc acgcctgggg catgaccgag atgagcccgc tcggcagcgc ctgcacgctg
     1921 atcccggaat acgcggagct tacgggcgac gcgcgcctcg acatccagat gaagcaggga
     1981 cagccgccgt tctcgatcga gatgaagatc accgacgacg agggccgcga gctgccgtgg
     2041 gacggcacga cgttcggccg cctcaaggtg cgcgggcccg caatcgccaa gtcctatttc
     2101 aagggcgaag gcggcgatat cctcgacgag aacggcttct tcgacaccgg cgacgtcgcc
     2161 accatggacc cccacggcta tatgcagatc acggaccgtg ccaaggacgt gatcaagtcg
     2221 ggtggcgagt ggatatcgtc gatcgacctg gagaacctcg cggtcggcca tccgtcggtc
     2281 gcagaggcag ccgtgatcgg catcaggcac ccgaaatggg atgagcggcc gctactggtc
     2341 gtggtgctca agtccgggca gaaggcgacg cgcgacgaca ttctcggctt catggtcggc
     2401 aagatcgccc attggtggat gcccgacgac gtcgtgttcg tcgacgagat tccgcacacc
     2461 gcgaccgga
//
LOCUS       DASRWS010000377         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4178705, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000377 DASRWS010000000
VERSION     DASRWS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4178705"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFF72_07065"
     CDS             <1..240
                     /locus_tag="VFF72_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HZV92961.1"
                     /translation="MARALDMVVQPVVAEILPGGQGYRVRFEAPWSDFPSDDAVADSA
                     RMNRWIESEIRRMPAQYLWVHRRFKTRPVGEPSLY"
     gene            357..1217
                     /gene="dapF"
                     /locus_tag="VFF72_07070"
     CDS             357..1217
                     /gene="dapF"
                     /locus_tag="VFF72_07070"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZV92962.1"
                     /translation="MRVRFTKMQGAGNDFVVLDATREPFALDSAQLKALADRRFGVGA
                     DQVLVIDKASRPGVDFGYRIFNGGSGDEVEQCGNGARCFVRYVRDKGLSDKRTLVVET
                     MNRRLQLHLQADGRVTVDMGAPDFDPAHSFFDITWLAPRAGDAGLPLWPVEIGEGRTV
                     DLAVVSMGNPHAVQVVADVEAAPVAAQGPRIEKHVRFARGVNAGFMEIVSRSRIRLRV
                     WERGAGETLACGTGACAAVAVGIRLGLLDAKVDVETHGGRLTIEWQGATAPVLMTGPA
                     ETVFEGEIEL"
     gene            1220..1918
                     /locus_tag="VFF72_07075"
     CDS             1220..1918
                     /locus_tag="VFF72_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HZV92963.1"
                     /translation="MTPAGTPHGVQGITEVDIADYLAASPGFFERHAELLGTIQLTSP
                     HGQRAVSLQERQMEMLRDKIKGLERQVIEMLRNGQDNVLIADKLHRWTRALMLTGNAA
                     DLPGVLVRELKHQFQIPQAAIRVWAGAEAFASLAFASPVGDDLKIFAGSLVVPYCGAN
                     AGFEAVGWLDDPAQASSIALVPLRHGGSEDAFGLLVLASPDPTRYTADMGTDFLARIG
                     EIASAALTRLLPAT"
     gene            1919..2911
                     /locus_tag="VFF72_07080"
     CDS             1919..2911
                     /locus_tag="VFF72_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZV92964.1"
                     /translation="MDARRDGSERGDPVGAGRAETPVAADADVGRHLEHLRVERRLAA
                     RSLVIYEDAFARLQRFAVAADVPLREVAVHHVRRWAAQLHAGGLSPRSIALVLSAWRG
                     LYRWLGREGRVAHNPVDGVRAPKAARPLPKALSVDHAVALVEHRGEGDETALGLRDAA
                     IFELLYGCGLRVGELVGLDVRAGAGVAGWIDEADASAHVLGKGGKRRGVPVGRPALQA
                     LARWLACRGSIARTGEPALFVSRRGTRLTPSQVRTRLKARAIAAGVPAHVHPHMLRHS
                     FASHVLQSSGDLRAVQELLGHANITTTQIYTKLDFGHLSKVYDAAHPRARKRAR"
     gene            2908..>3197
                     /locus_tag="VFF72_07085"
     CDS             2908..>3197
                     /locus_tag="VFF72_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZV92965.1"
                     /translation="MTGANAASHPRLLGDVGGTHARFAWVESAGAGFSRHASFDCDDH
                     DGLQQVIARYLDEQRLAAPPAAAIGIATPVTGDRVSMTNRDWSFSIDAVTKA"
BASE COUNT          450 a         1153 c         1143 g          451 t
ORIGIN      
        1 atggcgcgcg cgctcgacat ggtggtgcag ccggtggtgg ccgagatcct gcccggcggc
       61 cagggctacc gcgtgcgctt cgaggcgccg tggagcgact tcccgagcga cgacgcggtg
      121 gccgacagtg cccgcatgaa ccgctggatc gaaagcgaga tccggcgcat gccggcgcaa
      181 tacctctggg tgcatcggcg cttcaagacg cggcctgtcg gcgagccgtc gctgtactga
      241 gcccgctcga ccgtcgcggc cagccggagc aaggggtcgc gcgagccacg cggcgttgcg
      301 gatcgccttg gcgcctcgtg ccggccgggc gacgacccga acgcgataat ccggtgatgc
      361 gggttcgctt caccaagatg cagggcgcgg gcaacgactt cgtcgtcctc gacgcgacgc
      421 gcgagccgtt cgccctcgac agcgcgcagc tgaaggcgct cgccgaccgg cgcttcggcg
      481 tcggcgccga ccaggtcctc gtcatcgaca aggcgtcccg gcccggcgtc gatttcggct
      541 accgcatctt caacggcggc agcggcgacg aggtcgagca atgcggcaac ggcgcccgct
      601 gcttcgttcg ctacgtgcgc gacaaggggc tgagcgacaa gcgcacgctc gtcgtcgaga
      661 cgatgaaccg gcgcctgcag ttgcacctgc aggccgatgg ccgtgtcacc gtcgacatgg
      721 gggcgcccga cttcgatccg gcgcactcct tcttcgacat cacctggctc gcgccgcgcg
      781 cgggcgatgc cggactgccg ctctggccgg tcgagatcgg cgagggtcgc acggtcgatc
      841 tcgcggtcgt ctcgatgggc aatccgcacg cggtgcaggt cgtcgccgac gtcgaggcgg
      901 cgccggtggc cgcgcagggc ccgcgcatcg agaagcacgt ccgtttcgca cggggcgtca
      961 acgccggctt catggaaatc gtctcacgct cgcgcatccg gctgcgggtc tgggagcgtg
     1021 gcgccggcga gacgctcgcc tgcggcacgg gcgcctgcgc ggcggtcgct gtcggcatcc
     1081 ggctcggcct gctcgatgcg aaggtcgacg tcgagacgca cggcggccga ctgacgatcg
     1141 agtggcaggg cgcgacggcg cccgtcctca tgaccgggcc ggccgaaacc gttttcgaag
     1201 gagaaatcga actgtgacca ttaccccggc aggaacgccg cacggcgtgc aaggcatcac
     1261 cgaggtcgac atcgccgact atctcgccgc gagcccgggc ttcttcgagc gccatgccga
     1321 gttgctcggc acgatccagc tgaccagtcc gcacggccag cgcgccgtct cgctgcagga
     1381 gcggcagatg gaaatgctgc gcgacaagat caagggcctc gagcggcagg tcatcgagat
     1441 gctgcgcaac ggccaggaca acgtcctcat tgccgacaag ctgcaccgct ggacgcgcgc
     1501 gctcatgctt accggaaacg ccgctgacct gcccggcgtg ctggtgcgcg agctcaagca
     1561 ccagttccag attccgcagg cggcgatccg cgtctgggcc ggcgccgagg ccttcgcctc
     1621 gctcgccttc gcgagtcccg tcggcgacga cctcaagatc ttcgccggca gcctggtcgt
     1681 gccgtattgc ggcgccaacg ccggcttcga ggcggtcggc tggctagacg atccggcgca
     1741 ggcatcgtcg atcgcgctgg tgccgctgcg ccacggaggc agcgaagatg ccttcggcct
     1801 gctcgtcctg gcgtcgcccg acccgacgcg ctacaccgcc gacatgggca ccgatttcct
     1861 cgcccgcatc ggcgagatcg cgagcgcggc gctgacacgg ctgctgccgg cgacctagat
     1921 ggacgcgcgc cgcgacggca gcgagcgcgg cgaccccgtc ggggccggcc gcgccgagac
     1981 gcccgtcgcg gccgatgccg acgtcggccg ccatctcgag cacctgcgcg tcgagcgacg
     2041 cctggcggcg cgctcgctcg tcatctacga agatgccttc gcccgcctgc agcgtttcgc
     2101 cgtcgccgcc gacgtgccgc tgcgcgaagt ggcggtgcat cacgtgcggc gctgggcggc
     2161 gcaactgcat gccggcggcc tgtcgccgcg cagcatcgcc ctggtcctgt cggcgtggcg
     2221 gggcctgtac cgatggctgg gccgcgaagg tcgcgtcgcg cacaacccgg tcgacggcgt
     2281 tcgcgccccg aaggcggcgc ggccgctgcc caaggccttg tccgtcgatc acgcggtcgc
     2341 tctcgtcgag catcgcggcg aaggcgacga gacggcgctc ggcctgcgcg acgcggcgat
     2401 cttcgagctg ctctacggct gcggcctgcg cgtcggcgag ctggtcgggc tcgacgtccg
     2461 ggccggcgcc ggcgtggctg ggtggatcga cgaagccgat gccagcgcgc acgtgctcgg
     2521 caaggggggc aagcgacgcg gcgtgccggt cggccggccg gcgctgcagg ccctggcccg
     2581 ctggctcgcg tgccgcggca gcatcgcccg caccggcgag cctgcgcttt tcgtcagccg
     2641 ccgcggcacg cgactgacgc cgagccaggt gcggacgcgc ctgaaggcgc gggccatcgc
     2701 cgcgggcgtg ccggcgcacg tccatccgca catgctgcgg cactcgttcg catcgcacgt
     2761 gctgcagtcg agcggcgacc tgcgcgcggt ccaggagctc ctcggccacg ccaacatcac
     2821 gacgacccag atctacacca agctcgactt cggccacctc tcgaaggtct acgacgccgc
     2881 gcatccacgg gcgaggaagc gggcgcgatg acgggcgcca acgcggcttc ccatccgcgc
     2941 ctgctcggcg acgtcggcgg cacccacgcg cgcttcgcct gggtcgagtc ggcgggcgca
     3001 ggcttctcgc gccacgccag cttcgactgc gacgaccacg acggcctgca gcaggtcatc
     3061 gcccgctacc tggacgagca gcgcctggcg gcgccgccgg cggctgcgat cggcatcgcg
     3121 acgccggtca cgggcgatcg ggtgagcatg accaatcgtg actggagctt ctcgatcgac
     3181 gcggtcacga aggcgat
//
LOCUS       DASRWS010000378         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2729242, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000378 DASRWS010000000
VERSION     DASRWS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2729242"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFF72_07090"
     CDS             <1..711
                     /locus_tag="VFF72_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92966.1"
                     /translation="HHYGEAIEIYTRAIETHPDEPRLYRHRGHRFITIRKFDLAIRDL
                     QKAAELVEGTPDQVEPDGRPNDRNIPTSTLQTNIYYHLGLAHYLKAEYPQALEAYKKC
                     LSLSKNADMQVATSAWLYLTLRRLNHEDDARKVLQPITSSMDIIENAPYQRLLLFFKG
                     ELTESDLTGAKGTEGTIYAYGLANRALWSNDRDKAKNDMKRIVEKRENDWPSFAYIAA
                     EAGLVRLQKGEHKHKKKK"
     gene            complement(713..2041)
                     /gene="rseP"
                     /locus_tag="VFF72_07095"
     CDS             complement(713..2041)
                     /gene="rseP"
                     /locus_tag="VFF72_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZV92967.1"
                     /translation="MFLVQSILAFVFVLGVLIFVHELGHFLMARRIGVRVLTFSLGFG
                     PKLLSFRRGDTEYCISAIPLGGYVKMAGENPEDTRTGADDEFLSKSKWQRFQVLVMGP
                     VMNILLAIVVMALVLYQGAQLPLYEQQPVVIGAFSPNSVGEKAGLRLGDRIVTIDGKT
                     IDTWEQFSMAIVSKAKREVSVGFVRDGQNQNLNLVPEAQGKFEMGDIGIQPLIHPQVE
                     DVSKGQPAEEAGLQGGDVIMAVGGETNVGYEHLLAAIKSHSHQPLPLTIKRGDRLMEI
                     TVTPRPNGPQVMIGAHIKPLETHTVEPGPLESVTLSLTKNWEWTRLIVQTLAGLFTRE
                     TSVKQLMGPVAIADLSGTAAQAGLIQLFSLMAMISLNLGLLNLMPVPVLDGGHIFILA
                     LEGLARRDFSMKVKEKMLLAGFVLLLMLMVTVIYNDLMRIQWIEKLVPWR"
     gene            complement(2056..3243)
                     /locus_tag="VFF72_07100"
     CDS             complement(2056..3243)
                     /locus_tag="VFF72_07100"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZV92968.1"
                     /translation="MKSIAILGSTGSIGQSALAVVDAHPDRLRVVGLAAGGNVEKFAA
                     QVRRYRPSCAAMATSAALEGACAAMKSDGVDAPEAVHAGVEGLIAVATHPDADVVLFA
                     SSGTAALDAVLAAIDAGKTIALANKEILVMAGSLVMAAAARRGVAVLPVDSEHNAIHQ
                     CLHGRDPHDIQRLILTASGGPFRGLPADALAHVSAADALRHPTWRMGPKITVDSATLM
                     NKGLEVIEARWLFGVRPEQIDVLVHPQSIVHSMVELVDGSVIAQLGVTDMRLPIQYAF
                     SYPERWTAPLPSLDLARAGRLEFEPPDTTRFPCLDLACRALRGSPGLPIVLNAANEIA
                     VAAFLDDRLPFMGIPEVIRQAMDAYELERPQDVRSLNDVRAIEAWGREFADRGTRRLQ
                     SMS"
     gene            complement(3240..>3614)
                     /locus_tag="VFF72_07105"
     CDS             complement(3240..>3614)
                     /locus_tag="VFF72_07105"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZV92969.1"
                     /translation="VIIVVSDSAQYYTGRMLGRRKLAPAISPKKTIEGAIGGMIFGTA
                     ATIIVGRLVFVATPPGLLALLGATIVALGIVGDLFESLLKRSAGVKDSSALIPGHGGV
                     LDRIDSWLFAAPAYYVFVRYLQ"
BASE COUNT          718 a         1173 c         1142 g          581 t
ORIGIN      
        1 catcattacg gcgaagcaat cgagatctac acgcgcgcca tcgaaacgca ccccgacgag
       61 ccgcggctgt accgtcatcg tggacaccgg ttcatcacga tcaggaaatt cgatctcgcg
      121 atcagggacc tgcagaaagc agccgagctg gtcgaaggaa cgcccgatca agtcgagccc
      181 gatggtcgcc ccaacgatcg aaacattccg acgagcacgc tgcagacgaa catctactat
      241 cacttagggc tggcgcacta cctgaaggcc gaatacccgc aggcactcga ggcgtacaag
      301 aagtgcctga gcctgtcgaa gaacgccgac atgcaggtcg cgacgtcggc gtggctctat
      361 ctgacgctga ggcggctgaa tcacgaagac gacgcgcgca aggtgctgca accgatcacg
      421 tccagtatgg acatcatcga aaacgctcct taccagcgcc tgctgctctt tttcaaaggg
      481 gaactaacgg agagcgacct cacgggtgcc aaaggcaccg aggggacgat ctacgcctac
      541 ggcctcgcga atcgggcgtt gtggagcaac gatcgcgaca aggcgaagaa cgacatgaag
      601 cggattgtcg aaaaacgcga gaacgactgg ccgtcgttcg cgtacatcgc cgcggaagcg
      661 ggcctggtgc ggctacagaa gggggagcac aaacacaaga agaagaagtg acctaccgcc
      721 aggggacgag cttctcgatc cactggatgc gcatcaggtc gttgtagatc accgtcacca
      781 tcagcatcag cagcaggacg aaacccgcca gcagcatctt ctctttcacc ttcatgctga
      841 agtcgcggcg cgcgagcccc tcgagcgcga ggatgaagat gtgcccgcca tcgaggaccg
      901 gcacgggcat cagattcaac agccccaggt tcaggctgat catcgccatc aggctgaaga
      961 gctgaatcaa gccagcctgc gcggcggtgc cagagagatc tgcgatcgcc acggggccca
     1021 tgagctgttt caccgatgtc tcgcgggtga acagaccggc aagcgtctga acgatcaggc
     1081 gcgtccattc ccagttcttg gtgaggctca acgtgaccga ctcgagcggc cctggctcga
     1141 cggtatgcgt ttcgagcggt ttgatgtgcg cgccgatcat cacctgcggg ccgttcggcc
     1201 gaggcgtgac cgtaatctcc atcaggcgat cgccacgctt gatcgtcagc ggcaacggct
     1261 gatgagaatg cgacttgatt gccgcgagga ggtgctcgta accgacattg gtttcgccgc
     1321 cgacggccat gatgacatcg ccgccctgga gacccgcttc ctctgccggc tgccccttgc
     1381 tgacgtcctc gacctgcgga tggatgagcg gctggatgcc gatgtccccc atctcgaact
     1441 tgccctgcgc ctcgggcacc aggttcagat tctggttctg accgtcccgg acgaacccga
     1501 ccgagacctc gcgcttggcc ttcgacacga tggccatcga gaactgttcc cacgtgtcga
     1561 tcgtcttgcc gtcgatcgtg acgatgcggt cgccgaggcg gagaccagct ttctcgccga
     1621 cggaattcgg ggagaacgcc ccgatgacga ccggctgctg ctcgtagagc ggcagctgcg
     1681 cgccctgata gagcacgagg gccatcacga cgatggcgag caggatgttc atcaccgggc
     1741 ccatgacgag cacctggaag cgctgccact tcgacttcga caggaactca tcgtcggccc
     1801 cggtccgcgt gtcttcgggg ttctcccccg ccatcttcac gtaaccgccg agcgggatgg
     1861 cgctgatgca gtactcggtg tcgccgcgcc ggaagctgag gagcttcggc ccgaatccga
     1921 gcgagaacgt cagcacgcgg accccgatcc ggcgagccat gaggaagtgc ccgagctcgt
     1981 gcacgaaaat cagcacgccc agcacgaaga caaaggcaag aatgctctgc accaggaaca
     2041 cctgtatatc cggatttacg acatagattg tagcctacga gtcccgcggt cggcgaactc
     2101 gcggccccac gcctcgattg cccgcacgtc gttgagactg cgcacgtcct gcgggcgctc
     2161 gagctcgtag gcatccatcg cctggcgaat gacttcgggg attcccatga acgggaggcg
     2221 atcgtcgagg aaggcggcga cggcaatctc gttggcggcg ttgaggacga tcggcaggcc
     2281 gggactgccg cgcagagcgc gacacgccaa atccaggcac gggaagcgcg tcgtgtcggg
     2341 cggctcgaac tccaggcggc cggcgcgcgc gaggtccagc gacggcaagg gggccgtcca
     2401 tcgttcgggg tacgagaacg catactggat cgggagccgc atgtcggtca ccccgagctg
     2461 cgcgattacc gatccgtcaa ccagctccac catcgaatgc acgatcgatt gcgggtgaac
     2521 gagcacgtcg atttgctccg gacggacgcc gaacagccat cttgcttcga tgacctcgag
     2581 gcccttgttc atcagcgtcg ccgaatcgac ggtgatctta gggcccatgc gccaggtcgg
     2641 atgacgcagt gcgtccgcgg ccgagacgtg cgcgagcgcg tcggccggca gcccgcgaaa
     2701 gggaccgccg gacgcggtca ggatcagccg ctggatgtca tggggatcgc ggccgtgcag
     2761 gcactggtgg atcgcgttgt gttcgctgtc gacggggagg acggccacgc ctcgccgtgc
     2821 ggcagccgcc atcaccagcg agcccgccat gaccaggatt tccttgttgg ccagcgcgat
     2881 cgtcttgccg gcatcgatcg cggcgagcac cgcgtcgagc gctgccgtgc ctgacgaagc
     2941 gaacagcacg acgtcggcgt ccgggtgcgt ggcgaccgcg atcagcccct cgacgcctgc
     3001 gtgcaccgcc tccggcgcgt cgacgccatc gctcttcatt gctgcgcagg cgccctcgag
     3061 cgccgcgctc gtcgccatcg cggcacacga cggccggtag cgccgcacct gcgccgcgaa
     3121 cttctcgacg ttgccgccgg ccgccaggcc gacgacgcgc agccgatcgg gatgcgcgtc
     3181 gaccaccgca agcgcgctct gaccgatcga acccgtcgac ccgaggatcg cgatcgactt
     3241 cattgcaggt accgcacgaa cacatagtag gccggcgccg cgaacagcca gctgtcgatg
     3301 cggtcgagta cgccgccatg gcctggaatg agcgccgagg aatccttcac gcccgcactg
     3361 cgcttgagca gcgactcgaa gagatcgcca acgatgccga gcgcgacgat ggtggcgccg
     3421 agcaacgcca gcaatccggg cggcgttgcc acgaacacga gacggccgac gatgatggtg
     3481 gcggccgtgc cgaaaatcat cccgccgatc gccccttcaa tcgtcttctt cgggctgatg
     3541 gctggcgcca gctttcgacg tcccagcatg cggcccgtgt agtactgggc cgaatcgctc
     3601 acgacaatga tgac
//
LOCUS       DASRWS010000379         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1605375, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000379 DASRWS010000000
VERSION     DASRWS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605375"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..298
                     /locus_tag="VFF72_07110"
                     /pseudo
     CDS             1..298
                     /locus_tag="VFF72_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            496..3819
                     /locus_tag="VFF72_07115"
     CDS             496..3819
                     /locus_tag="VFF72_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZV92970.1"
                     /translation="MLPRYAELHCRSNFSFLTGASHPEELVLRAAALGYGALAITDEC
                     SLAGVVRAHVEAKAQGLHLVVGSEMRLVVPASGAAHARLVLLAQTRRGYGNLSHWITV
                     ARRRAAKGSYLAHPGDVEGKVPNAPMLAGLPECLALLVPSAAEPFEAVFAHAMWLKTW
                     FQDRAAIAVELLHRAGDDDLVDRIVRVAQFTGLPIVAAGDVLMHVRSRKPLQDTLAAT
                     RLGKPVAECGFALEPNAEQHLRSRGRLAALYEPAWLENTIGLAARCAFSLDELRYEYP
                     QEIVPEGETPASHLRKLTYAGAARRFGETALLEKYRPRIEDELAVIRALQYEAYFLTV
                     ADIVDWARAQGILCQGRGSAANSVVCYCLGVTEVDPERATLLFGRFISVERNEAPDID
                     IDFEHQRREEVIQYIYGKYGRHRAALTAVVISYRPRSALRDVGRALGIDLQRIEAVSK
                     SQHWFDGRSIAVERLRENGFDPESPVVRLWMELTAQLVSFPRHLSQHPGGFVIARGRM
                     AELVPVENATMKDRSVIQWDKDDLDALGVLKVDILAIGMLSAIRRALDDIGAKLGRAP
                     LLMQDIGDGDTATYDMICRADTVGVFQIESRAQMSMLPRLLPRCYYDLVVEVAIVRPG
                     PIQGGMVHPYLKNRGLRDDEVDCPDELKPALLRTKGVPIFQEQVMQIAMLAADFSAGE
                     ADALRRAMAAWKRRGGLSPFHERLVGRMVEKGYGREYAERIFKQIQGFGEYGFPESHA
                     AGFALLAYVSSWIKRHHPDAFLCGLLNAQPMGFYAPAQLVRDAREHGVEVRPVDVRTS
                     GWESSLEAATAEEERGHDGEAGRSSSPSPSPSPSPSPSPSSLPSSPSSSPGNARPLHL
                     AVGPLGQGAPLRPVRLGLGRINGLAEEAARRIVEARAEAPFASTEDLARRARLDAHAL
                     TLLANADALHGLDGHRHQAAWAVAGIDTRPTELLRATRTGEGPVRLAVPSEGESTLAD
                     YRALGLTLKRHPLALLRAKLASEFKVEPAARLRTFPHGRLARASGLVTHRQRPETAKG
                     TIFVTLEDETGAVNVIVWPKIAEQQRKPLLGATLLTVFGQWQRQGEGDEAVMHLLAAK
                     MIDHTALLGGLVSRSRNFR"
     gene            3875..>4405
                     /locus_tag="VFF72_07120"
     CDS             3875..>4405
                     /locus_tag="VFF72_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate-dependent dioxygenase"
                     /protein_id="HZV92971.1"
                     /translation="MSSTQLVTAELRRWIVAQAAAGHSPESVLEAMKKSGWSEDVALD
                     ALETTMRDHLLQLDGKLPADAPAATPVPGGHLSAATPRLDAGDREVDLLLALERPRVV
                     VFGNLLSADECDEIIALATARLSRSETVETNTGASEVNDARTSDGMFFQPAEFAVCAR
                     LESRIATLLGWPLENGE"
BASE COUNT          656 a         1651 c         1502 g          596 t
ORIGIN      
        1 ggtcttcccg gccgaagcga tcagcagcga tccgcgcagc gatgcgatgc atcgccatca
       61 catccacgag aaacggctgc agcgcgacct gaaggtggct gtcgacacag ccggcatcgc
      121 caagccggcg acggtgcata ccctgcgcca ttcgttcgcc acgcacctgc tgcaggccgg
      181 caccgacatc cgcaccgtgc agagcctgct cggccatgcc gatgtcagca ccaccatgat
      241 ctacacgcac gtgctgaagt cggccgccgc cggagcggcg agcccgctcg acggcctgat
      301 gctgacggcg atgggcgatc acgacaacga caacgacgat gtcgatggcg ccgccggcga
      361 tcgccgcggc cggccgaaga catcgtcgtc gagcgacccg gacaagaacc ggtcggcggc
      421 gcatcccctt cacaccggca gctcgctcga caagccgcct cgcgcccgcg agcccattcc
      481 cccctatcgc ctgccatgct gccgcgctac gccgagcttc actgccgcag caacttcagc
      541 ttcctgacgg gagcctcgca ccccgaggag ctggtgctgc gggccgcggc gctcggctac
      601 ggcgcgctcg cgatcaccga tgagtgctcg ctggccgggg tggtgcgggc gcacgtcgag
      661 gccaaggcgc aggggctgca cctcgtcgtc ggcagcgaga tgcggctcgt cgtgccggcc
      721 agcggcgcgg cgcatgcgcg gctggtgctg ctggcgcaga cgcggcgcgg ctacggcaac
      781 ctctcgcact ggatcaccgt cgcccgccgg cgcgccgcca agggcagcta cctcgcgcat
      841 ccgggcgacg tcgagggcaa ggtgccgaac gcgccgatgc tggccggcct gcccgaatgc
      901 ctggcgctgc tcgtgccttc ggcggccgag ccgttcgagg cggtcttcgc ccacgcgatg
      961 tggctgaaga cctggttcca ggaccgcgcc gccatcgccg tcgagctgct gcaccgcgcc
     1021 ggcgacgacg atctcgtcga ccgcatcgtc cgggtcgcgc agttcaccgg cctgcccatc
     1081 gtcgccgccg gcgacgtcct catgcatgtg cgctcccgaa agccgctgca ggacacgctc
     1141 gccgccaccc gcctcggcaa gccggtcgcc gaatgcggct tcgccctcga gccgaacgcc
     1201 gagcagcacc tgcgctcgcg cggccggctc gccgcgctct acgagccggc gtggctcgag
     1261 aacacgatcg gcctggcggc gcgctgcgcc ttctcgctcg acgagctgcg ctacgaatat
     1321 ccgcaggaga tcgttcccga gggcgagacg ccggcctcgc acctgcgcaa gctcacctac
     1381 gccggcgcgg cgcggcgttt cggcgagacg gcgctgctcg agaagtaccg gccgcgcatc
     1441 gaggacgagc tcgccgtcat cagggcgctg cagtacgagg cctacttcct caccgtcgcc
     1501 gacatcgtcg actgggcgcg cgcccagggc atcctctgcc agggccgcgg cagcgccgcc
     1561 aactccgtcg tctgctactg cctgggcgtc accgaggtcg atccggagcg ggcgacgctg
     1621 ctcttcggcc gcttcatcag cgtcgagcgc aacgaggcgc ccgacatcga catcgatttc
     1681 gagcaccagc gccgcgagga ggtcatccag tacatctacg gcaagtacgg ccggcaccgc
     1741 gccgcgctca ccgcggtggt catcagctac cggccgcgct cggcgctgcg cgacgtcggc
     1801 cgggccctgg gcatcgacct gcagcgcatc gaggccgtct cgaagagcca gcactggttc
     1861 gacggccgca gcatcgccgt cgagcggctg cgcgagaacg gcttcgatcc cgagtcgccg
     1921 gtcgtgcgcc tgtggatgga gctgacggcg cagctcgtct cgtttccgcg ccacctctcg
     1981 cagcaccccg gcggcttcgt catcgcccgc ggccgcatgg ccgagctggt gccggtcgag
     2041 aacgcgacca tgaaggacag gagcgtcatc cagtgggaca aggacgacct cgacgcgctc
     2101 ggcgtcctca aggtcgacat cctcgccatc ggcatgctga gcgcgatccg ccgcgccctc
     2161 gacgacatcg gcgccaagct cggccgcgcg ccgcttctca tgcaggacat cggcgacggc
     2221 gacaccgcca cctacgacat gatctgccgc gccgacaccg tcggcgtctt ccagatcgag
     2281 agccgggcgc agatgagcat gctgccgcgc ctgctgccgc gctgctacta cgacctcgtc
     2341 gtcgaggtcg cgatcgtccg ccccggcccg atccagggcg gcatggtcca cccgtacctg
     2401 aagaaccgcg gcctgcgcga cgacgaggtc gattgccccg atgagctgaa gccggcgctg
     2461 ctgcgcacca agggcgtgcc gatcttccag gagcaggtga tgcagatcgc catgctcgcc
     2521 gccgacttct cggccggcga ggccgacgca ttgcgccgcg ccatggcggc gtggaagcgg
     2581 cgcggcggcc tgtcgccctt tcacgagcgg ctggtcgggc ggatggtcga gaagggctac
     2641 gggcgcgagt acgccgagcg catcttcaag cagatccagg gcttcggcga atacggcttt
     2701 ccggagagcc acgccgccgg cttcgcgctg ctcgcctacg tgagcagctg gatcaagcgg
     2761 caccaccccg acgccttcct gtgcggcctg ctcaatgccc agccgatggg cttctatgcg
     2821 ccggcgcagc tggtgcgcga cgcccgcgag cacggcgtcg aggtacggcc ggtcgacgtg
     2881 cgaacgagcg gctgggagag cagcctcgaa gcggcgacgg cggaggagga gagggggcat
     2941 gacggcgaag ccggcaggtc atcgtcgcca tcgccatcgc catcgccatc gccgtcgccg
     3001 tcgccgtcgt cattgccgtc atcgccatcg tcgtcgccgg gcaacgcgcg gccgcttcat
     3061 ctcgcggtcg gcccgctcgg ccagggtgcg ccgttgcggc cggtgcgtct cggcctgggc
     3121 cgcatcaacg gcctggccga ggaggcggcc cggcgcatcg tcgaagcccg cgccgaggcg
     3181 cctttcgcca gcaccgaaga cctggcccgc cgggcccgcc tcgatgccca tgcgctcacc
     3241 ctcctcgcca acgccgacgc gctgcatggc ctcgacggcc accgtcacca ggcggcgtgg
     3301 gcggtggccg gcatcgacac gcggccgacc gagctgcttc gcgccacgcg caccggcgaa
     3361 gggccggtcc gtctcgccgt gccgagcgaa ggcgagagca cgctcgccga ctatcgcgcc
     3421 ctcggcctca ccctcaagcg ccatccgctg gcgctgctgc gcgcgaagct ggcgagcgag
     3481 ttcaaggtcg agccggcagc gcgcctgcgc accttcccgc acggccggct ggcgcgggcc
     3541 agcggcctcg tcacgcaccg gcagcggccc gagacggcca agggcacgat cttcgtcacc
     3601 ctcgaggacg agaccggcgc ggtcaacgtc atcgtctggc cgaagatcgc cgagcagcag
     3661 cgaaagccat tgctcggggc gacgctgctc accgtcttcg gccagtggca gcgccagggc
     3721 gagggcgacg aggcggtgat gcatctgctc gccgcgaaga tgatcgacca cacggcgctg
     3781 ctgggcggcc tcgtcagccg cagccgcaat ttccgctgac ggcgcgagcc ggccgcgaag
     3841 ccggcctcgc cggcttgggc agaatcacgg ctcgatgagc agcactcaac tcgtcaccgc
     3901 cgaactgcgc cggtggatcg tcgcccaggc cgcggccggc cattcgcccg agtccgtcct
     3961 cgaggcgatg aaaaagagcg gctggagcga ggacgtcgcg ctcgacgcgc tcgagacgac
     4021 gatgcgcgac cacctgctgc agctcgacgg caagctgccg gccgacgcgc ccgcggccac
     4081 gccggtgccg ggcggccacc tctcggcggc gacgccgcgc ctcgacgccg gcgatcgcga
     4141 ggtcgacctg ctgctggccc tcgagcggcc gcgcgtcgtc gtcttcggca acctgctctc
     4201 ggccgacgag tgcgacgaga tcatcgccct ggcgacggcc cgcctgtcgc gctccgagac
     4261 ggtcgagacg aacaccggcg cgagcgaggt caacgacgcg cgcaccagcg acggcatgtt
     4321 cttccagccg gccgagttcg cggtctgcgc ccggctcgag tcgcgcatcg ccacgctgct
     4381 cggctggccg ctcgagaacg gcgaa
//
LOCUS       DASRWS010000380         8263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_153785, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000380 DASRWS010000000
VERSION     DASRWS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153785"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFF72_07125"
     CDS             <1..496
                     /locus_tag="VFF72_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92972.1"
                     /translation="NSRAVLGYDDATRTLWLRGAIELGAATEFLAALQTHPLTQTIAL
                     ESPGGYVSESQAMAGEILKRHLDTYAPEKSASACVDLFAAGEKRWAGPRTMFGLHRSG
                     HECRPDQGLTRADLRAASFLRERGVAEDFIDRAFETPYDRLWKPDVRTVLESGLATGV
                     REGG"
     gene            563..1027
                     /locus_tag="VFF72_07130"
     CDS             563..1027
                     /locus_tag="VFF72_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZV92973.1"
                     /translation="MPSHPAPSEGQADPERYSIDELSALAGVTPRTVRYYIAQGLLSR
                     PEGEKRGAHYLRPHLEQLLLVRRWTDAGLSLDRIRELIAGAPEDPPRRASPPGSVEVW
                     SRVTVADGLEVQLEPGRAGLSPEQVRAFVRGITALYRKVRADNKESDDESPR"
     gene            1011..3305
                     /locus_tag="VFF72_07135"
     CDS             1011..3305
                     /locus_tag="VFF72_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HZV92974.1"
                     /translation="MNHHDETQGFGLTCVEPGAPAPVLAGVQASGRLDGVLFELTLRQ
                     TYRNASSRVLEVVYTFPLPHQSVLLGFASELNGERKPGSILARRQAEGRYEEALAEGD
                     APVMLEALDGGLHTANIGNLKPGDEIVLEVRFAQLLAFEQGRLRLAIPTTIAPRYGNA
                     EQAGLQPQQVPQASLEAEYKLALSVTVAGALAGGTVESATHTFTRETVQDGIRLSLAD
                     GARLDRDVVIVVQPREPLPSLVVQARDTVSPDVPVVMMAALQPPVAEQRERLALKLLV
                     DCSGSMAGDSIDSARDALKGALAALGENDQLSISRFGSTVEHLQAPVACTPRVLRLLG
                     QAVNGIQADLGGTEMEGALRAVFGLPMKADAIGADVLLITDGQIWQAEEMIAAARASG
                     HRVFAIGVGSSPAEGVLRALAEATGGACEFATPGEALEAAAQRMLVRIRQQPWRDVRI
                     DWGAETAWQTTLPASVFGGDTVVAFAGVAARTASAVRLLAKNTGGETIEISRGEADAP
                     CPGDSLARIAAARRMVVADEPEALGLAINYQLLSQQTHCILVHERAEADKVKGEAELH
                     CVNSMLAAGWGATGAVRAPMAAAYQISRLARPQFARSVSVSDFHLSIASLEVEDSGSV
                     WADSADKEIASLEVIARAVADHLDGGGQVSDLGLNCGPLRMDTDACSALDQAAAIGAS
                     DGQALVLLAWWANSRPQGIGSASIAAALQPHVDALAGSLVSQCVELFERILGGHADSG
                     RSVSRATRLRRSLLRAFAPGNSRT"
     gene            3402..5090
                     /locus_tag="VFF72_07140"
     CDS             3402..5090
                     /locus_tag="VFF72_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92975.1"
                     /translation="MYFALQDGGELGLNVGRFDERQWRLRLRLAKTANCWDYAPLIDV
                     VFREPGFEVARFTGKTVSQWLANWQAWDNPHCERFMAWYEGLINRFLSVLARRPRVPD
                     DRYGFEVAPLPWISSLSAMLRASGPNRATAQQWSAAIRATTEKGVRSEELALSGVLAR
                     LQQCPNDAKLTKTQVLRLVDLSHALPKLVCESRFEFATRAGWRECCDRVSAREFRRRG
                     LSSVAHGLAHIRFRHRSLGWSIARTRFKDLLMNERDSWIVLDEKGRLVGASKTAFASS
                     DAAMEFAELQISCRFASWSKPQAAARWECFSLPGGDDYRELLIQLDDWPATYRPRHYR
                     TRNVLSHVRSSMRQVVDGRRVLFLDEIQSDWHADLHAQSKRATLESDGKVTDAPYKKE
                     WPLLTMKIMLWLAQRCGAQGLAWSTAELQEIRWLGHEPPGLLYRNTLPDAARMLAKTL
                     PVTMDATRMRVRTGSRTVALGSMGWEVRRRQGSPITKPFRTRIQAESFADQTGDFVVL
                     DVPVLWLDRLTPITSIPLFGARTASGWTSAPMFESTRGDARTHQPPERPMPASI"
     gene            complement(5083..6081)
                     /locus_tag="VFF72_07145"
     CDS             complement(5083..6081)
                     /locus_tag="VFF72_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZV92976.1"
                     /translation="MPRRPDNLETVRLAIELLRRIPRQSKVSAQDLHAQLSAAGIVRS
                     ERTIQRQLDMLSEHFDIEREDKNKPYGYRWKEGAAGFSLPSMTEKEALLLTLAEQHLR
                     NLLPSSVLNSMKGFFAQARRSLGPDQNAQLERQWLSKVRIVSTTQPLLPPPIREGVLE
                     EVGNALFGNRWLHVDYENAAGKRWSGDVMPLGLAQQGPSLYLVVRYRDLEGERNLALH
                     RIHSATASTLRFKRPEFELKAFDDEGQFGLGSGVRIKIGFDIEKDAGRHLLEARLSLD
                     QRVEELEDHYRITATVTESEWLWRWIRAFGKAVCAVKVNGRAVDPAAPQRRRRAPK"
     gene            complement(6118..8226)
                     /locus_tag="VFF72_07150"
     CDS             complement(6118..8226)
                     /locus_tag="VFF72_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV92977.1"
                     /translation="MTLRRPRARLDSSTGPDLLPIVRLWVLRLLVPLSAHRGVFNRHG
                     FQDEGLALALGFGKWTDDDAGQFDARAVRAELRKLHAASERSAASAKVPAQLHQNIGR
                     LADLVGLAEIDRRILEFAVMLQNERALDDTADYLGQLSSLKVYQTLAVVLGLPQSSVR
                     AALEGQGMLARSGLLALERSATSTLRGKLNLLSDRFADHVSSSSADPISLLRDTVLPS
                     QPGHLGLSDFDHLSASLEVLRPYLRKTLDTRRPGVNVLLYGSPGTGKNQFAKALTAEL
                     GCSLYEVSGEDADGDAVNGAMRLRAYRVAQSVLSRSSAVILFDEVEDVFEPSEPARGF
                     GSTPAKGKKAWINRVLEGNPVPAFWLTNSIDNIDGAFLRRFDMVLELPVPPRRQREKI
                     AHDACAGLLDVKGVARLAECEDLAPAVVARAASIVRFVADDLPASDPGKVLQRLIDQT
                     LEAQGHLRLLNTASNALPDTYDPMFIHADTDLTVVAEGIARAGSARLCLYGPPGTGKT
                     AWARWLASQLQKPLLVKRASDLMSMWVGGSEKQIARAFRRAEADGAVLLIDEVDSFLQ
                     ERRAAKHSWEVTQVNEMLTQMESFGEVFIASTNLIEGLDEASLRRFDLKVRFDYLLPK
                     QAWALLVRQAVVLGLSPPSAEDHGRRLNQLDHLTPGDFAAVARQHRFRPLVDTAKLVQ
                     ALEDEVVLKRQGSARRMGFV"
BASE COUNT         1456 a         2635 c         2717 g         1455 t
ORIGIN      
        1 gaactcgcga gccgtgctgg gctacgacga cgccacacgc acgctctggc ttcgcggcgc
       61 catcgagctc ggcgcggcaa cggaattcct tgcagctcta cagacccatc cgctcacgca
      121 gacgatcgct ctggagagcc cgggcggcta tgtctccgag tcgcaagcga tggccggcga
      181 gatcctgaag cggcatctcg acacctacgc gcccgagaag tccgcgagcg cctgtgtcga
      241 cctttttgcc gcgggcgaga agcgatgggc cgggccacga acgatgttcg gcctgcatcg
      301 gtcggggcac gagtgccggc ccgaccaggg cttgactcgg gccgacctgc gtgcggcgag
      361 cttcctgcgc gagcgcggcg tggcggaaga cttcatcgat cgtgccttcg agacaccgta
      421 cgaccgtctc tggaagcctg atgtccgaac ggtgctcgag agtggcttgg cgacgggcgt
      481 ccgtgaaggc ggctgatcgt cagctggacc atcgatcttt taagacactt ggctgtctgt
      541 tataagtgtc cgataatgag ctatgcccag ccaccccgcg ccttccgaag gtcaggccga
      601 tcccgagcgc tactcgatcg acgagttgtc ggcgcttgcc ggggtcacgc cacgcacggt
      661 ccgctactac atcgcccaag gcctgctcag ccgacccgag ggcgaaaagc gcggcgcgca
      721 ttacctgcgg cctcacctcg agcaattgct cttggtccgc cgctggaccg atgccggcct
      781 gagcctcgat cgcatccgcg agctgatcgc cggcgcaccc gaagacccgc cccgccgcgc
      841 ttcgccgcca ggctcggtgg aggtgtggag ccgggtcacg gtggccgacg ggctggaagt
      901 gcagctggag cctggccgcg ctggcctttc gcccgagcag gtgcgcgctt tcgtgcgcgg
      961 catcaccgcg ctgtaccgca aggtgcgcgc cgacaacaag gagagcgacg atgaatcacc
     1021 acgatgaaac ccaggggttc ggcctgacct gcgtcgagcc cggcgcgccg gcgcccgtgc
     1081 tggccggggt gcaggccagc ggccgcctcg acggggttct gttcgagctg accttgcgtc
     1141 agacctatcg caacgccagc agccgggtgc tggaggtggt ctacaccttt ccgctgccgc
     1201 accagtcagt gctgctgggc ttcgcctcgg agctcaacgg cgagcgcaaa cccggcagca
     1261 tcctcgcccg gcgccaggcc gagggccgat acgaagaagc gctggccgag ggcgacgcgc
     1321 cggtgatgct cgaggcgctg gatggtggct tgcacacggc caacatcggc aacctcaagc
     1381 ccggcgacga gatcgtgctc gaggtgcgct ttgcccagtt gctcgcattc gagcaaggcc
     1441 gcttgcggct ggccattccc accacgatcg caccgcgcta cggcaacgca gagcaagcgg
     1501 ggctgcaacc gcaacaggtg ccacaggcct cgctcgaggc ggaatacaaa ctggcgctct
     1561 cggtcaccgt ggcgggcgcg ttggccggcg gcacggtgga gtccgccacg cacacgttca
     1621 cacgtgagac ggtgcaagac ggcattcggc tgagcctggc cgacggcgcc cggctcgacc
     1681 gcgacgtggt gatcgtggtg cagccgcgtg aaccgttgcc ctcgctggtc gtccaggcgc
     1741 gtgacaccgt gtcgccagac gtaccggtgg tgatgatggc ggccctgcag ccgcccgtgg
     1801 ccgagcaacg cgagcgcctt gcgctcaagc tgctggtcga ctgctcgggc tcgatggccg
     1861 gcgacagcat cgactcggcc cgcgatgcgc tcaagggtgc gttggcagct ctgggtgaga
     1921 acgatcagct gagcatcagt cgcttcggca gcaccgttga gcacttgcag gcacccgtcg
     1981 cctgcacgcc gcgggtgctg cgtctgctcg gccaggccgt gaacgggatc caggccgatc
     2041 tgggcggcac ggaaatggaa ggcgcactgc gcgccgtgtt cgggctgccg atgaaggccg
     2101 acgcgattgg cgccgacgtc ttgctgatca ccgatggcca gatctggcag gccgaagaaa
     2161 tgattgccgc cgcgcgggcc agcgggcatc gcgtgtttgc gatcggcgtg ggctcgtcgc
     2221 cagccgaagg cgtgctgcgc gcgctggccg aggccacggg tggcgcctgc gagttcgcca
     2281 cgccgggaga ggcgttggaa gcggccgcac aacgcatgct cgtgcgcatc cgccagcagc
     2341 catggcgcga cgtgcgaatc gactggggtg cggagactgc ctggcagacc acgctgccgg
     2401 ccagcgtctt cggcggcgat accgtagtcg cgttcgccgg cgtggccgcg cgcactgcaa
     2461 gcgccgtgcg attgctcgcc aagaacaccg gtggcgagac gatagagatc tctcgcggcg
     2521 aggccgatgc gccctgccct ggggacagcc tcgcgcgcat cgcggcggcg cggcgcatgg
     2581 tggtggccga cgaaccggag gcgctgggcc ttgccatcaa ctatcagctg ttgagccagc
     2641 agacccattg catcctcgtg cacgagcgtg ccgaagcaga caaggtcaag ggcgaggccg
     2701 agttgcattg cgtcaactcg atgctggcgg cgggctgggg agcgacgggc gcggtcagag
     2761 cgccgatggc ggcggcgtac caaatctcgc gtttggctcg gccgcagttc gcgcgcagcg
     2821 ttagcgtgtc ggacttccat ctctctattg cgagcctcga agtcgaggat tccggctcgg
     2881 tctgggccga ttcggcagac aaggagatcg cgtcgctcga ggtcatcgcg cgcgctgttg
     2941 cagatcacct tgatgggggc ggacaggtgt cggatctggg cttgaactgc gggcccctgc
     3001 ggatggacac ggacgcatgc tcggccctcg atcaagccgc agccatcgga gcaagtgacg
     3061 ggcaagcgct tgtgttgctc gcctggtggg cgaactctcg tccccaagga atcggcagcg
     3121 cctcgatcgc cgccgcgttg cagccgcacg tcgatgcact ggcaggaagc ttggtcagcc
     3181 aatgcgtcga gttgttcgag cgcattctcg gcggccatgc ggacagcggc aggagcgtgt
     3241 cgagagcgac gcgtctacgg cgttcgctgc tgcgcgcttt tgcccccggc aactccagaa
     3301 catgaaccgc ctaacagccg caactccaca tcgacttcat tgccgtgtcg gctaggctct
     3361 agaccagatt cgcgtcgctg tcgggcatcg gacaaccggt catgtacttc gccttgcaag
     3421 atggcggcga gctcggcctc aacgtcggcc gattcgacga gcgtcagtgg cgcttgcggc
     3481 tgcgcctggc aaagacggcg aactgttggg actacgcgcc actcatcgac gtcgtctttc
     3541 gagaaccggg gttcgaggtt gcccgcttca ccggaaagac cgtatcgcag tggcttgcca
     3601 attggcaggc ttgggacaat ccgcactgcg aacgattcat ggcctggtac gaaggcctga
     3661 tcaatcggtt cttgtccgtc ctcgcgcgac gtccgcgcgt tcctgacgac cgctatggct
     3721 tcgaagtggc tccgctgcca tggatatcct cgttgtcagc catgctgcga gcctccggcc
     3781 cgaatcgcgc cacggcgcag caatggtctg cagcgatccg ggccacgacc gaaaagggtg
     3841 tcagaagcga ggagctggca ctgtccggcg tactggcgcg tctccagcag tgccccaacg
     3901 acgccaagct tacgaagacg caggtgcttc gcctggtgga cttgtcccat gccttaccca
     3961 agctcgtctg cgagtcgcgc ttcgaattcg cgacccgggc gggatggcgg gaatgctgtg
     4021 atcgcgtgtc ggcacgcgag ttcagacgtc gaggcctttc cagcgtcgct cacggcctgg
     4081 cgcatatccg ctttcggcat cgctcactcg ggtggtcgat tgccagaacg cgtttcaagg
     4141 acttgttgat gaacgagcgc gacagttgga tcgtcctgga cgagaagggg cgactcgtcg
     4201 gcgcatcgaa gaccgccttc gcatcgtccg acgcggcaat ggagttcgct gaacttcaga
     4261 tcagctgccg ctttgccagt tggagcaagc cccaggccgc agcccggtgg gagtgctttt
     4321 ccttgcccgg aggcgatgac taccgcgagc tattgatcca gctggacgac tggcccgcga
     4381 cctaccgccc gcggcactat cggacacgaa acgtgctctc ccacgtgcgc agcagcatgc
     4441 gccaggtcgt cgacggtcgc cgcgtgcttt ttctggacga gatccagagt gactggcatg
     4501 ccgatctcca tgcccagagc aagagggcca cactggagag cgacggaaag gtgaccgacg
     4561 ctccgtacaa gaaggagtgg ccgctgctga cgatgaagat catgctttgg ttggcgcaac
     4621 gctgtggagc acaggggctt gcctggagca ccgccgaact ccaggagatc cgctggctcg
     4681 gccacgagcc gcccgggctg ctctaccgca acacgctccc agacgcagcg aggatgctgg
     4741 ccaagacgct acccgtcacc atggacgcca cgcgcatgcg cgtgcgaacc ggcagtcgaa
     4801 cagtcgcact cggatcgatg ggctgggaag tgcggcggcg tcaaggatct ccgattacca
     4861 agcccttccg aacacgtatt caggcggaaa gtttcgcgga ccaaaccggt gactttgtcg
     4921 tcctggacgt gccggttctc tggcttgacc gcttgactcc gatcacttcc atcccgctct
     4981 tcggcgcgag gaccgcgagc gggtggacga gtgcccccat gttcgaatcg acaaggggcg
     5041 acgcgcgcac acatcagcca cccgagcgcc cgatgccagc gtctatttag gcgcacgacg
     5101 tcgcctttgc ggcgccgcag gatcaaccgc ccgaccgttg accttgacgg cgcagacggc
     5161 ctttccgaac gcccggatcc aacgccacag ccattcggac tcagtcactg tggcggtgat
     5221 tcgatagtgg tcttccagct cttcgacccg ctgatcaagg gacagccgag cttccagcag
     5281 atggcgtccg gcatccttct cgatgtcgaa gccgattttt attcggacac cggagccaag
     5341 cccgaactgc ccctcgtcat cgaaggcctt gagctcgaac tcgggcctct tgaaccttag
     5401 ggtcgaggcc gtggctgaat gaattcggtg cagggcaagg ttgcgctcgc cctcgagatc
     5461 ccgatagcgc accacgagat aaagactcgg cccctgttgt gcaagcccga gcggcatgac
     5521 gtcgccggac cagcgcttgc cggcggcatt ctcatagtcg acatgcagcc agcggttgcc
     5581 gaacagtgcg ttacccactt cctcaagaac accttcacgg atcggcggcg gaaggagcgg
     5641 ctgagtcgtg ctcacgatcc gcaccttgct cagccactgc ctttcgagtt gtgcattttg
     5701 gtccgggccc aggcttcgcc tcgcctgcgc gaagaagcct ttcatggagt tgagcacgct
     5761 ggacggcaag agatttcgaa gatgctgctc cgccagagtc aaaagaagcg cctccttctc
     5821 cgtcatgctg ggcaacgaga accctgccgc cccctccttc catcggtagc cgtaaggctt
     5881 gttcttgtct tcacgctcga tgtcgaagtg ctcgctgagc atgtcgagct gtcgctggat
     5941 ggtgcgttcg ctgcgaacaa ttccggccgc ggatagctgg gcgtgcagat cctgcgccga
     6001 gaccttgctt tgacgtggaa tacggcgaag caactcgatt gccagccgga ccgtttcgag
     6061 gttgtcgggt cggcgcggca tgtcaggaac tctggtcgag gcgagtgcgg tgccgcatca
     6121 gacaaacccc atccggcggg cagagccctg gcgcttcagc acgacttcgt cctccaatgc
     6181 ctgcacgagc tttgcagtgt ctaccagcgg gcggaatcga tgctggcgag ccactgcggc
     6241 aaagtcgccc ggtgtgagat ggtcgagctg gttcaggcgc cttccgtgat cttctgccga
     6301 tggcggagac agcccgagca cgaccgcctg cctgacaaga agcgcccatg cttgcttggg
     6361 aagaagatag tcgaagcgca ctttgaggtc gaagcgccgc agggacgcct cgtcgagacc
     6421 ttctatgagg ttggtactgg cgatgaagac ttcgccgaag ctttccatct gggtcagcat
     6481 ctcgttcacc tgggtcactt cccagctgtg tttggcagcg cgacgctcct gaagaaaact
     6541 gtccacttca tcgatcagca gcacggcgcc gtcggcctcg gcgcgtctga aggctctggc
     6601 gatctgcttc tcgctgccgc caacccacat gctcatcaga tcggaggctc gcttcacaag
     6661 cagcggcttt tgaagctgtg acgccagcca gcgtgcccaa gcggtcttcc cggtgccggg
     6721 cggaccgtaa aggcacagcc gagccgaacc cgcacgagcg atgccctctg cgacgacggt
     6781 cagatcggtg tctgcgtgaa tgaacatcgg gtcgtaggta tcgggcaatg cgttcgaggc
     6841 ggtattgagc agtcggagat gtccttgggc ctcaagggtc tggtcgatca ggcgttgcag
     6901 aaccttgccg gggtcggaag caggcagatc gtcggccacg aagcgaacga tagaggccgc
     6961 gcgggccacg actgctggcg ccagatcctc gcactcggcc aggcgagcca cgcccttgac
     7021 gtcgagcagc cccgcgcaag cgtcgtgcgc gatcttctcg cgctgtcgtc gcggcggaac
     7081 gggtaactcg agcaccatgt cgaagcgccg gagaaacgca ccgtcgatgt tgtcgatcga
     7141 gttggtcagc cagaacgccg gcacggggtt gccttccagg actcgattga tccaggcttt
     7201 cttgcccttg gccggtgtcg aaccgaagcc gcgcgcgggc tctgacggct cgaagacgtc
     7261 ttcgacttca tcgaacagga tcacggcgct cgaccgcgac agaacgctct gcgcaacgcg
     7321 ataggcgcgc agccgcatcg cgccgttgac ggcgtcgcca tccgcgtcct caccggacac
     7381 ttcgtagagc gagcagccga gctcggccgt gagggccttg gcgaactggt tctttccggt
     7441 gccggggctg ccgtagagca ggacgttgac gccggggcgc cgggtatcga gtgtcttgcg
     7501 caagtaaggc cgaagcactt ccaacgaagc tgacaaatga tcgaagtcgg acaaaccgag
     7561 atggcctggc tgcgacggca gaacggtgtc gcgtaagagg gagattgggt cggcgctcga
     7621 cgacgatacg tgatccgcga aacggtcaga aagcagattc agcttgccgc gcaaggtgct
     7681 cgttgcgctt cgctccaatg ccagaagtcc ggagcgggcc aacataccct ggccttccag
     7741 cgccgcgcgc acgcttgact gcggcaagcc gagaacgacg gccaacgttt ggtagacctt
     7801 cagcgacgac aactggccta agtagtctgc ggtgtcgtcg agtgcgcgct cgttttgcag
     7861 catgaccgcg aactcgagaa tccgccggtc gatctccgcg aggccgacga ggtcggctag
     7921 acggccgatg ttctgatgca gttgcgcagg cacctttgcg ctggcagcgc ttcgctccga
     7981 cgcagcgtgc agtttgcgca gttctgcccg cactgcccgc gcatcgaatt gcccggcgtc
     8041 gtcgtctgtc cacttgccga agccgagggc aagcgcaaga ccttcgtcct ggaagccgtg
     8101 acggttgaag acccctcggt gggcgctcag cggcacgagc aatcggagca cccagagacg
     8161 cacgatcggg agcaggtcgg gccctgtcga tgaatcaagg cgagcgcggg ggcgacgaag
     8221 ggtcattcat atctcctggc acgggagaag tatcgccatt cgt
//
LOCUS       DASRWS010000381         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_457416, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000381 DASRWS010000000
VERSION     DASRWS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457416"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..654
                     /locus_tag="VFF72_07155"
     CDS             190..654
                     /locus_tag="VFF72_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV92978.1"
                     /translation="MTARTVLFASTLAASILTAFATVSAHGEDAAGPTRDQVKADFLH
                     ARATGHAMPAGEASAVFEDASTSPALTRADVRGEFALARTAGQLVPRGDATSPIDDGS
                     VSLLSRAQVRDEVRVAQARGELMPAGEAYATPNRQARRATSDAMVVATRSTH"
     gene            807..1118
                     /locus_tag="VFF72_07160"
     CDS             807..1118
                     /locus_tag="VFF72_07160"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZV92979.1"
                     /translation="MILEIADIRIQTGRHAEFEAAIRRGAETVIAQAKGHVSHRVVKG
                     IESPDRYVLMIWWQTLEDHTEGFRSSPLFAQWRSIVGPFFAQPPSVEHFSTVMQSQAD
                     A"
     gene            1115..2548
                     /locus_tag="VFF72_07165"
     CDS             1115..2548
                     /locus_tag="VFF72_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZV92980.1"
                     /translation="MTRARVVRVGLARVARAGAALLVAATLPGCATTAPPTGDATGAT
                     TTTQAATPIDLKVPRIAFRERRLGNGLQVISVQSHASPTVAIEVSYHVGGRDDPPGRS
                     GFAHLFEHMMFKSTKYMPAEQFDRMTEDVGGSNNASTGDDVTEYHEVVPSNHLETLLW
                     AEAERMQNLKVDQGNFTSERAVVEEEYRQRILASPYGRFFSAVTSRPYLHSPYQRPTI
                     GSIADLDAATLADVAAFHSLHYRPDNATLVVSGDFDQRQLDAWVDKYFGPIPRPAEPL
                     PARHADEPPWTSDRTIEVTAPRVPLPATALVWLGPTVTSADAPALEVAAAILSGGDSS
                     RLNQALVYRQQIAAQAGFSADLRVGPGLLVASAIAAGGKPIDAVRGALLAEVLRLADA
                     PPSSAEIDKVKNQLITDAFASRQTPMGLASAIADAAVLEGDANRVNTDLADLQRVTPA
                     DVQRVMHRYVVGRHKVVLDYRQEGSPK"
     gene            2554..4017
                     /locus_tag="VFF72_07170"
     CDS             2554..4017
                     /locus_tag="VFF72_07170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZV92981.1"
                     /translation="MARASWVAGAAAIAFAGIASAQPYPFPPPAAPPRPLTIAAPVEQ
                     SLANGLRVITARRDDVPLVSVLLVVLSGSEADPPALSGLASMTAGLLTQGTTRHTAPQ
                     LAAAAEALGGSLDSSAGWGQSTVSITVTTPKLDAALGLVAEVAREPVFAQEEIDRLRA
                     QMLDGLKVSYANPSRIATLAAVRLAYGASAWGHPAGGTPTSLPRIVRADLVAAHAVAF
                     RPDRAVLILSGDIDPSTALSLARARFGSWQAPATSPAPVPVVPAAAAPGPALTVIDMP
                     SASQAGVTMTLPLPPRRDNEWATGAVLNAVLGGGYSSRLNQEIRIKRGLSYGADSYIS
                     GRERSGLFVASVQTKNETAAQVVGLAQAEIDTMAAAPVGAAELAARKAMIVGGFSRSV
                     ETTAGLASAIDGLVVSGRSPALLTTQIDSIEKVDADAVQRYAAAHLGVAGRRVVVAGV
                     ADRIVGELSSGGAPVDVIRADQLDLDRVDLKRPATAR"
BASE COUNT          601 a         1498 c         1379 g          581 t
ORIGIN      
        1 gccattggcg cggtcgcggc acggtgaacg ggccgccgcg acgatgcctc ggcgaaacag
       61 gagtgaattt ccgcactcgt ccgcagcgta ctcatcactc ggatcggtcg cgtcgccgcc
      121 ctagattgac ttcgcccgcc aatggttgac gggtgccgat cccggcaaac gcatcgtttg
      181 gagactccca tgactgcacg caccgttctt ttcgcatcga ccttggcggc ttccatcctg
      241 accgccttcg ccacggtctc ggcacatggc gaagacgcgg cggggccgac ccgcgaccag
      301 gtcaaggccg atttcctgca cgcccgcgcc accggccatg ccatgcccgc cggcgaggcg
      361 tcggcggtct tcgaggacgc gtcgacgagc ccggcgctga cgcgcgccga cgtccgcggc
      421 gaattcgcgc tggcccggac ggccggccag ctcgtgccgc gcggcgacgc gacctcgccg
      481 atcgacgacg gcagcgtctc gctgctcagc cgcgcacaag tgcgcgacga ggttcgcgtg
      541 gcgcaggccc gcggcgagct gatgcccgcc ggcgaggcct atgcgacgcc gaaccggcag
      601 gcgcggcgcg cgacgagcga cgcgatggtg gtcgcgacgc gcagcacgca ctgaaggccc
      661 ggcgcgcctg gcccggtcgg cggacgcgct gcgaatcctg gagcagcacg ccatccggtc
      721 gggtcttcgg cgcgcccaaa tccctgctcc tctggcccgg cggacccgcc gctagactgg
      781 ccggctccga gccaacgggt caccgcatga ttctcgaaat cgccgacatc cgcatccaga
      841 ccggccgaca cgccgagttc gaagccgcca tccggcgcgg cgccgagacg gtcatcgcgc
      901 aagccaaggg tcatgtctcg caccgcgtcg tcaagggcat cgaatcgccc gatcgctacg
      961 tgctcatgat ctggtggcaa accctcgaag atcacaccga aggctttcgc agcagcccgc
     1021 tgttcgcgca atggcgctcc atcgtcggcc cgttcttcgc gcagccaccc tcggtcgaac
     1081 acttttcgac ggtgatgcag tcgcaggcag acgcgtgacg cgagcgcgag tcgttcgggt
     1141 cggcctggcg cgcgtcgctc gtgcgggcgc ggcactcctg gtcgcagcga cgctgccggg
     1201 ctgcgccacc accgcgccgc cgacgggcga cgcgaccggc gcaacgacga cgacgcaagc
     1261 cgccacgccg atcgacctga aggtgccgcg catcgccttt cgcgagcggc gcctcggcaa
     1321 cgggctgcag gtcatctcgg tgcaaagcca cgccagcccg accgtcgcca tcgaggtctc
     1381 gtaccacgtc ggcggtcgcg acgacccgcc ggggcgctcc ggcttcgcgc atctcttcga
     1441 gcacatgatg ttcaagagca cgaaatacat gccggccgag cagttcgacc gcatgaccga
     1501 agacgtcggc ggctccaaca acgcctcgac cggcgacgac gtgaccgagt accacgaggt
     1561 cgttccttcg aaccacctcg agaccctgct ctgggccgaa gccgagcgga tgcagaacct
     1621 gaaggtcgac cagggcaact tcacctcgga gcgcgccgtc gtcgaagagg aataccgcca
     1681 gcgaatcctc gcctcgccgt acggacgctt cttcagcgcc gtcacgtcgc ggccctacct
     1741 gcattcgccc taccagcggc cgacgatcgg cagcatcgcc gacctcgacg cggcgacgct
     1801 ggccgacgtc gccgctttcc attcgctcca ctaccggccc gacaacgcca ccctggtcgt
     1861 cagcggcgac ttcgaccagc gccagctcga cgcctgggtc gacaagtact tcggaccgat
     1921 cccccgcccg gccgagccac tgcccgcgcg acacgccgac gagccgccgt ggacgagcga
     1981 ccgcacgatc gaggtgacgg cgccgcgcgt gccgttgccg gcgacggcgc tcgtctggct
     2041 cgggccgacg gtgacgagcg ccgacgcacc ggcgctcgaa gtggctgccg cgatcctttc
     2101 aggcggcgac tcgtcgcgcc tcaaccaggc gctcgtctat cgacagcaga tcgccgcgca
     2161 ggccggtttc agcgccgatc tgcgggtcgg gcccggactg ctcgtcgcca gcgcgatcgc
     2221 ggcgggcggc aagccgatcg acgccgttcg cggcgccctg ctggccgagg tgctgcgcct
     2281 ggccgacgcg ccgcccagca gcgccgagat cgacaaggtg aagaaccagc tcatcaccga
     2341 cgccttcgcg tcgcgacaga cgcccatggg cctggcctcg gcgatcgccg acgccgcggt
     2401 gctcgaaggc gatgcgaacc gcgtcaacac cgacctcgcc gacctgcagc gggtcacgcc
     2461 tgccgacgtg cagcgcgtga tgcaccgcta tgtcgtcgga cggcacaagg tcgttctcga
     2521 ctatcggcag gaagggagcc cgaagtgaag gcgatggcac gcgcctcgtg ggtcgccggc
     2581 gctgccgcga tcgcctttgc cggcatcgct tcagcgcagc cttacccctt tccaccgccg
     2641 gcggcgccac cgcggccgtt gacgatcgcg gcgccggtcg agcagtccct tgccaacggt
     2701 ctgcgcgtga ttaccgcgcg acgcgacgac gtgccgctgg tgagcgtgct gctcgtggtt
     2761 ctttccggca gcgaagccga tccgccagcg ctctcgggcc tggcctcgat gacggccggc
     2821 ctgctcacgc aaggcacgac gcgtcacacc gccccgcagc ttgccgccgc ggccgaggcg
     2881 ctgggtggct cgctcgacag cagcgccggc tggggccagt cgacggtctc gatcacggtg
     2941 acgacaccca agctcgacgc cgcgctcggc ctcgtcgccg aagtcgcgcg cgagcccgtg
     3001 tttgcgcagg aggagatcga tcgcctgcgc gcgcagatgc tcgacgggct gaaggtgagc
     3061 tacgccaatc cctcgcgcat tgccacgctg gcggcggtgc ggctcgccta cggggcgagc
     3121 gcctggggac acccggcggg cggcacgccg acatcgctgc cgcggatcgt ccgcgccgat
     3181 ctcgtcgccg cgcatgcggt ggcctttcgg cccgatcgcg ccgtgctgat cctctccggc
     3241 gacatcgatc cgtcgacggc gctgtcgctg gcccgcgccc gcttcgggtc gtggcaggcg
     3301 ccggcgacct cgcctgcgcc ggtgcctgtg gtgccggcag cggccgcgcc gggtccggcg
     3361 ctgacggtga tcgacatgcc gtcggcgagc caggccggcg tcaccatgac cttgccgctg
     3421 ccgccccggc gtgacaacga gtgggcgacc ggcgcggtgc tcaacgccgt gctcggtggc
     3481 ggctattcgt cgcggctgaa ccaggaaatc cggatcaagc gcggcctcag ctacggagcc
     3541 gacagctaca tctcgggccg cgagcggagc ggtcttttcg tggcgtcggt gcagacgaag
     3601 aacgaaacgg cggcccaggt ggtggggctg gcccaggccg agatcgacac catggcggcg
     3661 gcaccggtcg gcgccgcgga gcttgcggca cgcaaggcga tgatcgtcgg cggcttcagt
     3721 cgcagcgtcg agacgacagc gggcctggcc tcggcgatcg acggtctcgt cgtctccggc
     3781 cggtcgccgg ccttgctgac gacgcagatc gattcgatcg agaaggtcga tgccgatgcc
     3841 gtgcagcgtt acgcggcggc ccatctcggt gtcgccggac ggcgcgtcgt cgtggccggt
     3901 gtcgccgatc gcatcgtcgg cgagctgtcg tccggcggcg caccggtcga cgtgattcgc
     3961 gccgaccagc tcgatctcga ccgcgtcgac ctgaagcgtc ctgccaccgc gcgctgacgc
     4021 ggaaatcgcg cggctcgacg cgaccggatc gacgcgcct
//
LOCUS       DASRWS010000382         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1867558, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000382 DASRWS010000000
VERSION     DASRWS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867558"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFF72_07175"
     CDS             <1..297
                     /locus_tag="VFF72_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV92982.1"
                     /translation="RGADFDFVITDIGMPAMNGFELLAAIKKDERLRHLPVLMVTAEA
                     KRDDIVRCAQDGAAGYIVKPFSAAVLGERLRRAAPRLFAAPSAAAATAAPVGPA"
     gene            580..>2126
                     /locus_tag="VFF72_07180"
     CDS             580..>2126
                     /locus_tag="VFF72_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZV92983.1"
                     /translation="MSSVPAKPEAAGVFPVGRSLLWATLLIGALTAAAVVATYRTHRR
                     TAAARVEVIADLRAAQVSRWLRERVDAAGFLAGSQPTADYYLHWQAGDAASGKRLVAR
                     MVDVRTSTGYQSMRILDAEGTVVASESGSDATTPPELRDAARRAMASGRVERTQPYGY
                     TGPVPAPRIDVVVPLNGTDRPARAAVALRVDPRQWLFPTLRAWPVPTRSGGSIVVWRD
                     GADVVGAVNGRHVPLAADTLATRVLRGTAPRNVALDGLDYRGRPVLGIVSPIEGTDWF
                     LVAKIDLAEVRDAAIGDVVLIVGIGVLSLLVVAVAVFRYRDRQALAVSRANEAAQAAR
                     LRDGQLLDSIARESTDFIFAKDLQGRYLMFNREAGRVFGVDPQAMIGRLAVDALPGAE
                     AEVVGRHEAEVKAQGRTLVFEEHLTTGDGKRRFLITRGPLRDADGGIAGTFGIARDMT
                     ERIELEGRLREREAALLHSQVVARLGHVVSDADGRVDSVSEALPVLVGRPAAAMPRDF
                     DALLEWV"
BASE COUNT          276 a          770 c          775 g          305 t
ORIGIN      
        1 cgcggcgcgg acttcgattt cgtcatcacc gacatcggca tgccggcgat gaacggcttc
       61 gaactgctcg cggcgatcaa gaaggacgag cgactgcggc acctgcccgt cctcatggtc
      121 accgccgagg cgaagcgcga cgacatcgtt cgctgcgccc aggacggtgc tgccggctac
      181 atcgtcaagc cgttctccgc cgcggtgctc ggcgagcgcc tgcgccgcgc cgcgccgcgg
      241 ctgttcgcgg caccttcggc cgcggccgcg acggctgcgc cggtcggccc ggcctgagcc
      301 gtcgaggcgc aatcggcatc gcggtcgcgg tcgcggtcgc ggtcgcggcg atcgtcggga
      361 ccgtcgaggt cgtcgggctc gtcgggctcg tcgggctcgt cggcagccga cgcggcgccc
      421 gtgcgaactc gcgccgcgct tcggacgcga atgtcgtcaa gcctgtcacg ctcggcggcg
      481 ctgcgcaggg caatgcccgg ctgccgcgct gattccgcag tgcatcggct taggatggcc
      541 gcgatccgat tcccgccctg gcaaacgggc agacaccgag tgagcagcgt tccagcgaag
      601 cccgaagcgg caggagtctt tccggtcggc cgcagcctcc tctgggcgac gctgctgatc
      661 ggcgcgctga ccgcggcggc cgtcgtcgcg acctatcgaa cccatcgccg cactgccgcg
      721 gcgcgggtcg aggtgatcgc cgacctgcgc gcggcgcagg tctcgcgctg gcttcgcgag
      781 cgtgtcgacg ccgccggttt cctcgcgggc agccagccga ccgccgacta ctacctgcat
      841 tggcaggccg gcgacgcggc aagcggcaag cgcctcgtcg cccggatggt cgacgtgcgc
      901 acctcgaccg gctaccagag catgcgcatt ctcgatgccg aaggaaccgt cgtcgcgagc
      961 gagtcgggca gcgacgccac cacgccaccc gagttgcgcg atgcggcgcg ccgggcgatg
     1021 gcgagcggcc gggtcgagcg aacgcagccg tatggctaca ccggccccgt gccggccccg
     1081 cggatcgacg tcgtcgtgcc gctcaacggc accgaccggc cggccagggc cgccgtcgcg
     1141 ctgcgcgtcg atcctcgcca gtggctgttc ccgacgctgc gcgcgtggcc ggtgccgacg
     1201 cgcagcggcg gctcgatcgt cgtctggcga gacggtgccg acgtggtcgg cgcggtcaac
     1261 ggccggcacg tgccgctcgc cgcggacacg ctcgccacgc gcgtccttcg cggcaccgca
     1321 ccgcgcaacg tggcgctcga tgggctcgac tatcgcggcc ggccggtgct cggcatcgtc
     1381 agcccgatcg agggcaccga ctggttcctg gtggccaaga tcgacctggc cgaggtgcgc
     1441 gacgccgcca tcggcgacgt cgtcctgatc gtcggcatcg gcgtgctgtc gctgctggtc
     1501 gtggcggtgg ccgtgttcag gtatcgggac cggcaggcgc tcgcggtatc gcgggccaac
     1561 gaggccgcgc aggcagcgcg ccttcgcgac ggccagcttc tcgactcgat cgcccgcgag
     1621 tcgaccgact tcatcttcgc caaggacctg cagggccgct acctgatgtt caaccgcgag
     1681 gcgggccgcg tcttcggcgt cgatccgcaa gccatgatcg gacgcctggc ggtcgacgcc
     1741 ctgccgggcg cggaagcaga ggtcgtcggc cgccacgagg ccgaggtgaa ggcgcagggc
     1801 cgcacgctgg tcttcgagga acacctgacg accggcgacg gcaagcggcg gtttctcatc
     1861 acgcgcggac cgcttcgcga cgccgacggc gggatcgccg gcaccttcgg catcgcgcgc
     1921 gacatgaccg agcggatcga gctggagggt cgcctgcgcg agcgcgaggc ggcgctgctg
     1981 cactcgcagg tcgtggcccg gctcggacac gtcgtcagcg acgccgacgg ccgcgtcgac
     2041 agcgtctccg aggccttgcc ggtgctggtc ggcaggccgg ctgccgcgat gccgcgcgac
     2101 ttcgatgcgt tgctcgaatg ggtcca
//
LOCUS       DASRWS010000383         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3893740, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000383 DASRWS010000000
VERSION     DASRWS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893740"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VFF72_07185"
     CDS             complement(<1..1761)
                     /locus_tag="VFF72_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV92984.1"
                     /translation="MLVLSQLSLRRGTKLVLDRASVVINAGEKVGLVGRNGAGKSSLF
                     SLLAGRLGADAGDLSLPPRWRLAEVAQEMPETGEGATDFVLAGDTRLAEANAALAAAE
                     ASDDGHAMAEAHHAISDAGGFDARPRAQAMLMGLGFRSADLDAPVNSFSGGWRMRLQL
                     ARALMCPADLMLLDEPTNHLDLDALVWLEAWLKRFGGTMIVISHDREFLDAVTDVTLH
                     LDDARLTRYTGNYTAFEEMRAIRLTQAAASYEKQRERMAHLQKFIDRFKAKASKAKQA
                     QSRVKALARMEKLAPVLTSADFQFEFREPLSLPNPMLAFAGLACGYRGGDAGDVGTEK
                     RVVEGVDRSVLAGQRIGILGANGQGKSTLVKTIAGELSPLAGGIVEGKGLKIGYFAQQ
                     ELDVLSPADGPLMHMVRLARDLGPPGREQELRDFLGSFRFTGEMVIQPVGTLSGGEKA
                     RLVLAMLVWQRPNLLLLDEPTNHLDLATREALSMALNEFEGTVMLVSHDRALLREVCD
                     EFWLVAAGALAPFDGDLDDYQRWLLEQSKAAARAAKAAPAAMPSAPAAGTVATAPPGE
                     QAGSSSPAPAASGRGRDERKA"
     gene            complement(1895..2455)
                     /locus_tag="VFF72_07190"
     CDS             complement(1895..2455)
                     /locus_tag="VFF72_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZV92985.1"
                     /translation="MPKIADIEDTPNPNALKFTLREPLSWGVSHSYDNAAQAAGDELA
                     SALFAIEHVTNVFYVDRWLTVTQDGEADWKELVREIAVPLRAAPGAEAQSGATIAAAK
                     AGAEPLSERDQDRLDEINVLLDEQIRPYLRGDGGDLHVLSLEGDRLLVHYQGACGTCP
                     SSISGTLRGIENLLRTIEPNIEVVAA"
     gene            complement(2480..2968)
                     /locus_tag="VFF72_07195"
     CDS             complement(2480..2968)
                     /locus_tag="VFF72_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZV92986.1"
                     /translation="MGTDPKSLYQEVILDHNRKPRNYGELEHPSHRAEGLNPLCGDHI
                     HVAVDVADETVEAIAFHGESCAICKASASMMTTAVKGKRREDAQALARDFVAMATGRL
                     APADVARVGRLAVFSGISDLPMRVKCAILPWHTLQAAFNAEATTTTEADADPMHTPIG
                     DA"
     gene            complement(2974..>3995)
                     /locus_tag="VFF72_07200"
     CDS             complement(2974..>3995)
                     /locus_tag="VFF72_07200"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZV92987.1"
                     /translation="GYEAARRTLSRFINAREEREVIFTSGTTEGINLVAHGWGRKFVG
                     AGDEIILTVLEHHSNIVPWQMLAAEKGATIRVVPINDAGELLVGEYEALFNARTRIVA
                     VGHVSNALGSVNPVERMIAFAHARGVPVLIDGAQAAPHLAVDVQALDCDFYAFSGHKM
                     CGPTGIGVLYGRAHLLEAMHPFKGGGDMIHTVTFEKTTYNTIPHKFEAGTPPIAAAIG
                     LGAAVDYLSGLGMASIAAHEHALLRHATDRLRDLPGVRIVGTAHDKAAVLSFAIDGIH
                     PHDVGTLLNQEGVAVRTGHHCAQPLMKRLGVSATSRASFAFYNTLAEVDALVAGIRSV
                     QKVFA"
BASE COUNT          571 a         1343 c         1429 g          652 t
ORIGIN      
        1 cgccttgcgc tcgtcgcggc cacggccgga tgcggccggc gccggcgacg aagaaccggc
       61 ctgctcgccg ggcggcgccg tcgcgacggt gccggccgcc ggtgcggaag gcatcgccgc
      121 gggcgccgcc ttcgccgcgc gcgccgcggc cttcgattgc tcgagcagcc agcgctggta
      181 gtcgtcgagg tcgccgtcga agggggcgag cgcgcccgcg gccaccagcc agaattcgtc
      241 gcagacctcg cgcagcagcg cccggtcgtg gctcaccagc atcacggtgc cttcgaactc
      301 gttgagcgcc atgctcagcg cttcgcgcgt cgcgaggtcg aggtgattcg tcggctcgtc
      361 gaggagcagc aggttgggcc gctgccagac gagcatggcc aggacgaggc gtgccttctc
      421 gccgcccgag agcgtgccga ccggctggat caccatttcg ccggtgaagc gaaacgagcc
      481 gaggaaatcg cgcagctcct gctcccttcc tggcggaccg aggtctcgcg cgaggcgcac
      541 catgtgcatc agcggcccgt cggcaggcga gagcacgtcg agttcctgtt gcgcgaagta
      601 gccgatcttg agccccttgc cttcgacgat gccgccggcc agcggcgaaa gctcgccggc
      661 gatggtcttg accagcgtcg acttgccctg gccgttggcg ccgaggatgc cgatccgctg
      721 acccgcgagg accgagcggt cgacgccttc gacgacgcgc ttttccgtgc cgacatcgcc
      781 cgcgtcgccg cctcgatagc cgcaggcgag cccggcaaag gcgagcatcg ggttgggcag
      841 gctcagcggc tcgcggaact cgaactggaa atcggcgctg gtcagcaccg gggccagctt
      901 ttccatccgg gccagcgcct tgacgcggct ttgtgcctgc ttggccttgc tcgccttggc
      961 cttgaaccgg tcgatgaact tctgcaggtg cgccatccgc tcgcgctgct tctcgtagct
     1021 ggccgcggcc tgcgtcaggc ggatcgcgcg catctcctcg aacgcggtgt agttgcctgt
     1081 gtagcgagtc agccgcgcat cgtcgagatg cagcgtcacg tcggtgaccg cgtcgaggaa
     1141 ctcgcggtcg tggctgatga cgatcatcgt tccgccgaag cgcttcagcc acgcttcgag
     1201 ccagaccagg gcgtcgaggt cgaggtggtt ggtcggctcg tcgagcagca tcaggtcggc
     1261 cggacacatc agggcgcggg cgagctgaag gcgcatccgc cagccgccgg aaaagctgtt
     1321 caccggcgca tcgagatcgg cgctgcgaaa gcccaggccc atcagcatcg cctgggcgcg
     1381 gggccgggcg tcgaagccgc cggcatcgct gatcgcatga tgggcctcgg ccatcgcgtg
     1441 gccgtcgtcg ctcgcctcgg cggccgcgag cgccgcattc gcctcggcca gccgcgtgtc
     1501 gcccgcgagg acgaagtccg tcgcaccttc cccggtctct ggcatctcct gcgcgacttc
     1561 ggcgagtcgc cagcgtggcg gcagcgacag atcgccggcg tcggcgccga ggcgtccggc
     1621 cagcagcgag aagagcgacg acttgccggc gccgttgcgg ccgaccaggc cgaccttctc
     1681 gccggcgttg atgacgacgc tggcgcgatc gagaacgagc ttcgtgccgc gacgaagcga
     1741 aagctggctg agaacgagca tggggaaaag gtcgactgat gttctgtggc gggtcaaggg
     1801 cagcgccatc gcgcgacctc attcgcctgg gcgctggccg gcgcgtgccc gccggcgcaa
     1861 ggagcggtcg cgacgacgcg gattgtgccg gcgactacgc cgcgacgacc tcgatgttcg
     1921 gctcgatcgt gcgcagcagg ttctcgatgc cgcgcagcgt tccggagatc gagctcgggc
     1981 aggtgccgca ggcgccctgg tagtgaacca gcagtcgatc gccttcgagg ctgaggacat
     2041 gcaggtcgcc gccgtcgccg cgcaagtagg gccggatctg ctcgtcgagc aggacgttga
     2101 tctcgtcgag ccggtcctgg tcgcgttcgc tcaggggctc ggcaccggcc ttcgccgcgg
     2161 cgatggtggc ccccgactgg gcctcggcgc cgggcgcggc gcgcagcggc acggcgatct
     2221 cgcggacgag ctccttccag tcggcctcgc cgtcctgcgt cacggtgagc cagcgatcga
     2281 cgtagaaaac gttggtcacg tgctcgatcg cgaacagcgc gctcgccagc tcgtcgcccg
     2341 cggcctgggc ggcgttgtcg tacgaatgcg agacgcccca cgacagcggt tcacgcagcg
     2401 tgaacttcag tgcgttcgga ttcggggtgt cttcgatgtc ggcgatcttc ggcatgttgg
     2461 tgtggcgggg tcgagccgtt caggcgtcgc cgatcggcgt atgcatcgga tcggcgtcgg
     2521 cctcggtggt ggtggtcgcc tcggcgttga aggcagcctg cagggtgtgc cacggcagga
     2581 tcgcgcactt cacccgcatc ggcaggtcgc tgatgcccga gaagaccgcg aggcggccga
     2641 cgcgggcgac gtcggccggc gcgaggcgcc ccgtcgccat ggcgacgaaa tcgcgcgcca
     2701 gcgcctgcgc gtcctcgcgt cgcttgccct tgacggcggt cgtcatcatc gacgccgacg
     2761 ccttgcagat cgcgcacgac tcgccgtgga aggcgatggc ctcgaccgtc tcgtcggcga
     2821 catcgacggc gacatggatg tgatcgccgc acagcggatt caggccctcc gcgcggtggc
     2881 tcggatgctc gagctcgccg tagttgcgcg gcttgcggtt gtggtcgagg atcacctcct
     2941 ggtagaggga tttcggatcg gtgcccatgg cgctcacgcg aataccttct gcacgctgcg
     3001 gatgccggcg acgagcgcat cgacctcggc cagggtgttg tagaacgcga acgacgcgcg
     3061 cgaggtcgcg gacacgccga gccgtttcat cagcggctgc gcgcagtggt ggccggtgcg
     3121 cacggcgacg ccttcctggt tcagcagcgt gccgacgtcg tgcgggtgga tgccgtcgat
     3181 cgcgaacgac agcacggcgg ccttgtcgtg tgccgtgccg acgatgcgca cgccgggaag
     3241 atcgcgcagc ctgtcggtag catggcggag cagcgcgtgc tcgtgcgcgg cgatcgatgc
     3301 catgccgagc cccgacaggt agtcgacggc agcgccgagg ccgatcgccg cggcgatcgg
     3361 cggcgtcccg gcctcgaact tgtgcgggat cgtgttgtag gtggtctttt cgaacgtcac
     3421 cgtgtggatc atgtcgccgc cgcccttgaa cggatgcatc gcttccagca gatgggcgcg
     3481 tccgtagagc acgccgattc cggtcgggcc gcacatcttg tgtcccgaga aggcgtagaa
     3541 atcgcagtcg agcgcctgca cgtcgacggc gaggtgcggc gcagcctgcg cgccgtcgat
     3601 cagcaccgga acgccgcggg cgtgcgcaaa cgcgatcatc cgctcgaccg ggttgacgct
     3661 gcccagggcg ttcgagacgt ggcccacggc gacgatgcgc gttcgcgcgt tgaagagcgc
     3721 ttcgtactcg cccacgagca gctcgccggc gtcgttgatg ggaacgacgc ggatcgtcgc
     3781 ccccttttcg gccgccagca tctgccacgg cacgatgttc gagtgatgtt cgagcaccgt
     3841 caggatgatc tcgtcgcccg cgccgacgaa cttgcggccc cagccgtgcg cgaccaggtt
     3901 gatgccctcg gtggtgccgc tcgtgaagat cacctcccgc tcttcgcggg cgttgatgaa
     3961 gcgcgacaac gtgcgccggg ccgcctcgta tccgg
//
LOCUS       DASRWS010000384         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3601651, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000384 DASRWS010000000
VERSION     DASRWS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601651"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFF72_07205"
     CDS             <1..265
                     /locus_tag="VFF72_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZV92988.1"
                     /translation="DANTHLHVSFDVDFLDADIAPGVGTTVKGGPTYREAQLCMEMIA
                     DTGAMASLDVMELNPALDVRNRTAEIAVDLIESLFGKSTLMRR"
     gene            complement(334..804)
                     /locus_tag="VFF72_07210"
     CDS             complement(334..804)
                     /locus_tag="VFF72_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV92989.1"
                     /translation="MTDPVASLGIRSLQPADFDAWQPLWRGYNAFYGREGPTALAPEI
                     TAVAWQRFLDPGEPVFALVAEAEGRLLGLAHFLFHRSTTRIELTCYLQDLFTDPAVRG
                     RGVGRALIEGVYERARAAGIRRVYWQTHETNAAGRLLYDKVARHAGFIVYSQDL"
     gene            complement(860..1357)
                     /locus_tag="VFF72_07215"
     CDS             complement(860..1357)
                     /locus_tag="VFF72_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZV92990.1"
                     /translation="MAIDPDELAARMDGIEPAKGSIGLGVRRAFDRLEEAMLEGSCLC
                     GAARWRFDRLPESATACNCTACRRYGALWAYDYEGAGIEVSGATKGYVRGTALAFHFC
                     SECGAMAYWRALGTDKEGRRRIAVNLRLADPDAVAGVPVDHFDGLVGWEDLPRDGRCV
                     KDLWF"
     gene            complement(1341..1862)
                     /locus_tag="VFF72_07220"
     CDS             complement(1341..1862)
                     /locus_tag="VFF72_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZV92991.1"
                     /translation="MSDASAFPVEGRCTCGEVRFRLESAPLVVHCCHCRWCQRETGAS
                     FALNAMIESDRVTNLGAPPELVDTPSQSGRGQAIARCRRCRVAVWSHYAGSGPVTKFV
                     RVGTLDRPGLLSPDVHIFTASKQPWVTLSPEVPTFAEYYDLETVWSAESLARRRALLP
                     LIEEYRASRGHRS"
BASE COUNT          389 a          821 c          769 g          314 t
ORIGIN      
        1 cgatgccaac acccacctgc acgtgagctt cgacgtcgat ttcctcgacg ccgacatcgc
       61 ccctggcgtc ggcaccaccg tcaagggcgg cccgacctat cgcgaggcac agctgtgcat
      121 ggaaatgatc gccgacaccg gcgccatggc ctcgctcgac gtgatggagc tcaacccggc
      181 cctcgacgtc aggaaccgca ccgccgagat cgccgtcgac ctgatcgaaa gcctgttcgg
      241 caagagcacg ctgatgcggc gctgacgtcg cgcgggatcg ggcgccggtt cggcaaggct
      301 cgtcgaagcg ccgctggccg acgcgccgga tcctcagaga tcctggctgt agacgatgaa
      361 accggcgtgt cgtgccacct tgtcgtagag caggcgcccg gccgcgttcg tctcgtgcgt
      421 ctgccagtag acgcggcgga tgccggcagc gcgggcgcgc tcgtagacgc cctcgatcag
      481 cgcccggccg acgccccggc cgcgcaccgc gggatcggtg aacaggtcct gcaggtagca
      541 ggtgagctcg atgcgcgtgg tgctgcggtg aaagagaaag tgcgcgagcc cgagcagcct
      601 gccctcggcc tcggcgacga gcgcgaagac cggctcgccg ggatcgagga accgctgcca
      661 ggcgaccgcc gtgatctccg gcgcgagtgc cgtcgggcct tcgcggccgt agaacgcgtt
      721 gtagccgcgc cagagcggct gccacgcgtc gaagtcggcc ggctgcagcg aacggatgcc
      781 gagcgaagcg accgggtcgg tcatggcagc acctcacggc gcccgttcgc gccaagcacg
      841 cagttgcggt ggacggaccc tagaaccaca ggtccttcac gcagcggccg tcgcgcggca
      901 ggtcttccca gccgacgagt ccgtcgaaat gatcgaccgg cactccagcc accgcatcgg
      961 gatcggcgag gcgcaaattc accgcgatcc ggcggcggcc ttccttgtcc gttccaagcg
     1021 cgcgccaata cgccatcgcg ccgcactcgc tgcagaagtg aaaggcgagc gcggtgccgc
     1081 gcacgtagcc cttcgtcgca cccgagacct cgatgccggc gccttcgtag tcgtaggccc
     1141 agagagcccc gtagcgccgg cacgcggtgc agttgcaggc cgtcgccgac tcggggagcc
     1201 ggtcaaacct ccatctcgcc gcgccgcaga gacacgaacc ttccagcatc gcttcctcca
     1261 accgatcgaa cgcacgccgg acaccgagcc cgatcgatcc tttcgcaggc tcgatgccgt
     1321 ccatgcgcgc cgcgagctcg tcaggatcga tggccacgtg acgcgcgata ttcctcgatg
     1381 agcggcaaga gcgcccgtcg cctcgccagg ctttccgccg accacacggt ttcgagatcg
     1441 tagtactccg cgaaagtggg cacctccggc gacagcgtca cccagggttg cttcgaggcg
     1501 gtgaagatat gaacgtcggg agacagaagg ccgggacgat cgagcgttcc gacgcgaacg
     1561 aacttcgtga ccggacccga gccggcatag tggctccaga cggccacccg gcagcgtcgg
     1621 cagcgcgcga tcgcctggcc gcgcccgctc tgcgaaggcg tgtcgacgag ctcgggcggc
     1681 gcgccgagat tcgtgacgcg atcggattcg atcatcgcgt tgagggcgaa cgaggctccg
     1741 gtctcgcgct ggcaccagcg gcaatggcag caatggacaa cgagcggcgc ggactcgagc
     1801 cggaagcgga cctcgccgca cgtgcagcgc ccttcgacgg ggaacgcgct ggcgtcgctc
     1861 atgggcgccc gttccgcagc ggcgcccgca cgcgccgcgc agcgcgcccg acgcggcgat
     1921 caggccgccg ggccgggcga cacgacgtgt tcttcgacat gacgcgcgca agcatattcg
     1981 aggcgacccg gcgcggccag ccttcgcgcg cgacgcgcgc cgcacttgcg cctcccaaga
     2041 ctcgcacgct tcgcacgact cgcacgactc gcacgactcg caagcctcgc acgacacatg
     2101 caacaccacg cgcgacgcag gcgacgcgtc gctacagcgg gtcggtgttc atcccgggca
     2161 gtccgcggcc gcgtcggctg ccaccggtgc tcgctcgacc agcttcttgc gcagcttgtg
     2221 ccatggcccg aagacatgga tcgaccggct cagctcgaag aggcccgggc gcttgggctg
     2281 cggcgtcaga agc
//
LOCUS       DASRWS010000385         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3338890, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000385 DASRWS010000000
VERSION     DASRWS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338890"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VFF72_07225"
     CDS             complement(<1..1042)
                     /locus_tag="VFF72_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZV92992.1"
                     /translation="MLSLPVIASGKRYTLPRPPGSADALLLAAFALQRRREDKAATTV
                     VFTAEPADTQRLLDELAFFAPALRVAEFPDWETLPYDTFSPHEDLISERLATLWKIGR
                     GEVDVVVMPATTALVRLAPPSFIAAYTFEFRQKQRLDEAALRAQLTLAGYSHVSQVVS
                     PGEYALRGGLVDLFPMGSSVPYRVDLFGDEIDSIRTFDPDSQRSLYPVPEVRLLPGRE
                     FPMDEAARTAFRSRWRERIEGDPTKVRLYKDVGTGIATAGIEYYLPLFFDETATIFDY
                     LGTAAALVLHGEVDAALQRFWSDTRERHRFLQHDKERPILAPEAIFLRPEDFFARCGE
                     RAQLLVRGRDDAG"
     gene            1183..1926
                     /gene="ispD"
                     /locus_tag="VFF72_07230"
     CDS             1183..1926
                     /gene="ispD"
                     /locus_tag="VFF72_07230"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZV92993.1"
                     /translation="MPDATFAPDRLHALVPCAGSGARAGAGRPKQHVVVAGRTVLAHT
                     LVALAEVQRLASILVVLAPGDAGGESLANNAVDDPRVTVARCGGATRAETVANGLDAL
                     RERGAAEGDWVLVHDAARCMIRADWIDRLIAACVADSVGGLLALPVADTLKSARAGRV
                     AATLDRRDVWQAQTPQMFRLGALRTALATALTKAGGTVTDEASAMEAAGHSPLLVPGE
                     LENFKLTQPGDFALAERLLSSRVVAGIAA"
     gene            1923..>2073
                     /locus_tag="VFF72_07235"
     CDS             1923..>2073
                     /locus_tag="VFF72_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZV92994.1"
                     /translation="MNRHRPAWRVGEGWDIHALVADRRLVLGGVEVPHRHGLLGHSDA
                     DVLCHA"
BASE COUNT          312 a          706 c          757 g          298 t
ORIGIN      
        1 cgccggcgtc gtcgcggccg cgaaccagca actgggcccg ctcgccgcag cgcgcgaaga
       61 agtcctccgg gcgcaggaag atcgcctcgg gggcgaggat cgggcgctcc ttgtcgtgct
      121 gcaggaagcg gtgccgctcg cgcgtgtcgc tccagaaacg ttgcagcgcc gcgtcgactt
      181 cgccgtgcag caccagcgcg gcggccgtgc cgaggtagtc gaagatcgtc gccgtctcgt
      241 cgaaaaagag cggcaggtag tactcgatgc cggcggtcgc gatgccggtg ccgacgtcct
      301 tgtacaggcg caccttggtc ggatcgcctt cgatccgctc gcgccagcgc gagcgaaagg
      361 cggtgcgggc cgcctcgtcc atcgggaatt cgcggccggg cagcaggcgc acctcgggga
      421 ccgggtagag gctgcgctgg ctgtcgggat cgaaggtgcg gatcgagtcg atctcgtcgc
      481 cgaagagatc gacccggtac ggcaccgacg agcccatcgg gaagaggtcg acaaggccgc
      541 ctcgcaacgc atattcgccc ggcgagacga cctggctcac gtggctgtag ccggccaatg
      601 tcagctgggc gcgcagcgcg gcttcgtcga gacgctgttt ttgccggaac tcgaacgtgt
      661 aggcggcgat gaagctcggc ggcgcgaggc gaaccagcgc cgtcgttgcc ggcatgacga
      721 cgacgtcgac ctcgccgcgg ccgatcttcc agagcgtcgc cagccgctcg gagatcaggt
      781 cttcatgcgg cgagaaagtg tcgtaaggaa gcgtctccca atcgggaaac tcggcgacgc
      841 gcaaggccgg cgcgaagaat gcgagctcgt cgaggagccg ctgcgtgtcg gcgggctcgg
      901 cggtgaacac gaccgtcgtc gccgccttgt cttcgcgccg acgctgcagc gcgaaggcag
      961 cgagcagcaa ggcgtcggcc gaaccgggcg gccggggcag cgtgtaccgc ttgccggaag
     1021 cgatgacggg aagagagagc atgaaggcgc gaaccgcgcc gttcgatgcg gcacgagaaa
     1081 gagtgcaatt ttagggacgc ggcggcatcg ctgccggcgg cggggcatgc cgcaaggagc
     1141 gaacccttat gcttgcggcc cccctcgccc acgtgtcgac gcatgcccga cgccactttc
     1201 gccccggacc gcctgcacgc gctcgttccc tgcgccggaa gcggcgcgcg cgccggcgcg
     1261 ggccggccca agcagcatgt cgtcgtcgcc ggccgcaccg tgctcgctca cacgctcgtc
     1321 gcgctggccg aggtgcaacg gcttgcgtcg atccttgtcg tgctggcgcc cggcgatgcc
     1381 ggcggcgaat cgcttgcgaa caacgccgtc gacgatcccc gggtaaccgt cgcacgctgc
     1441 ggcggggcga cgcgggccga gaccgtcgcc aacggcctcg acgcgttgcg cgagcgtggc
     1501 gcagccgaag gcgactgggt gctcgtccac gacgcggccc gttgcatgat ccgcgccgac
     1561 tggatcgatc gtctcatcgc cgcctgcgtt gccgacagcg tcggcggact gctcgccctg
     1621 ccggtcgccg acacgctcaa gtcggcccgc gccggccgtg tcgccgcgac gctcgaccgc
     1681 cgcgacgtgt ggcaggcgca gacgccgcag atgtttcgcc tcggcgcgct gcgtacggca
     1741 cttgccacgg cactgacgaa agccggcggc acggtgaccg atgaggcctc ggcgatggag
     1801 gcggcgggcc attcaccgct tctcgttccc ggcgagctcg agaacttcaa gttgacgcag
     1861 ccgggcgatt tcgcgctggc ggagcggctg ctgtcgagcc gcgtggtcgc ggggatcgcg
     1921 gcatgaaccg gcaccgcccg gcgtggcgtg tcggcgaggg ttgggacatc cacgccctcg
     1981 ttgccgaccg caggctcgtg ctgggcggcg tcgaggtgcc gcacaggcac gggctgctcg
     2041 gccattccga cgccgacgtc ctctgccatg cga
//
LOCUS       DASRWS010000386         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_735985, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000386 DASRWS010000000
VERSION     DASRWS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735985"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VFF72_07240"
     CDS             <1..159
                     /locus_tag="VFF72_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZV92995.1"
                     /translation="GVRCMAAGILDDQGNLVAGLSISAPADRLEESWLERLRVTAAQI
                     SASLGHRR"
     gene            complement(211..1227)
                     /gene="pbpG"
                     /locus_tag="VFF72_07245"
     CDS             complement(211..1227)
                     /gene="pbpG"
                     /locus_tag="VFF72_07245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HZV92996.1"
                     /translation="MSLACAAAAVAANNPPAKSKHPAARHAVATRHATTKVASRSRSR
                     KALVVTRSRQGRKHVVARIEPARPSFGQVYGLHNVSDSLDLKSSVALVLDQDTNEVLF
                     SKNSQAVLPIASLTKLMTALVITEAKLPLDEPITITDDDIDTEKFSRSRLKVGTTLRR
                     EEMLHLALMSSENRAAHALGRTYPGGLAVFVDAMNRKARELGMNDTHYVEPTGLSSQN
                     QSSAHDLALLVKTAHEFPLIRELSTSPEYQVEVGNRQVEYHNTNGLIRNPSWDIGLQK
                     TGYISEAGRCLVMQAKLAGRHLIMVFLDSAGKYSRIGDAERVRRWLTESTAALPIERS
                     KPKI"
     gene            complement(1416..>2932)
                     /locus_tag="VFF72_07250"
     CDS             complement(1416..>2932)
                     /locus_tag="VFF72_07250"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZV92997.1"
                     /translation="TMRDGEQSPGASMTRDEKLRIARSLERMRVDVIEAGFPASSNGD
                     FEAVRAIANTVRDSVVCGLCRANDRDIQRGIEALQGAKGPWRIHTFIATSALHMEKKL
                     RMTPEQVYEQAKLSVRFARKHCADVEFSPEDGSRSEPDFLCRVLEAVIAEGATTINIP
                     DTVGYAIPELYGEFLRDLRQRIPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQIE
                     CTINGLGERAGNCSLEEVVMAVKTRKDYFDLAIGVDATQIVPASRLVSQTTGFVVQPN
                     KAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSGRNAFRQRLK
                     ELGIEMESEADVNAAFVRFKELADRKSDIFDEDILALVMDEAVVADHDHYRLISLAQH
                     SETGARPHARVVFAAGGVEHGASSDGNGPVDASLRAIESKVASGAEMLLYSVNAITSG
                     STESQGEVTVRLQHGGRVVNGVGADPDIVVASAKAYLAALNKLHNKQERVAAQG"
BASE COUNT          476 a          965 c          960 g          531 t
ORIGIN      
        1 ggcgtgcgtt gcatggcggc cggcatcctc gacgaccagg gcaacctggt cgccggcctg
       61 tcgatctcgg cacccgccga tcgcctcgag gaaagctggc tggagcgcct gcgggtgacg
      121 gcggcgcaga tttcggcgtc gctgggccac cgccgctgag ctcgaatccg cgtcgcggac
      181 tgcgacagcg gaccgcgggc tcgcaagagc tcagatcttg ggcttcgatc tctcgatcgg
      241 cagggccgcc gtcgattcgg tcagccagcg gcgaacccgc tcggcgtcgc cgatgcggga
      301 gtacttcccg gccgaatcga ggaagaccat gatgagatgc cggccggcca gcttggcttg
      361 catgaccagg catcggccgg cctcggagat gtacccggtc ttctgaaggc cgatgtccca
      421 cgacggattg cggatcaggc cgttggtgtt gtggtactcg acctgccggt tgccgacctc
      481 gacctgatat tcgggcgacg tcgaaagctc gcggatgagc gggaactcgt gcgccgtctt
      541 gaccagcagc gccaggtcgt gcgcgctcga ctggttctgg ctcgacagcc ccgtcggctc
      601 gacgtagtgg gtatcgttca ttcccagctc gcgcgccttg cgattcatcg catcgacgaa
      661 gaccgccagg ccgcccggat acgtgcggcc aagggcgtgc gcggcccggt tctccgacga
      721 catcagcgcc agatggagca tttcctcgcg gcgcagcgtc gtgccgacct tcaaacgcga
      781 gcgactgaac ttctcggtat cgatgtcgtc gtcggtgatg gtgatcggct cgtcgagggg
      841 cagcttggcc tcggtgatca ccagtgcggt catcagcttc gtcagcgagg cgatcggcaa
      901 caccgcctgc gagttcttgc tgaaaagcac ctcgttggtg tcctggtcga ggacgagggc
      961 gacactcgac ttcaggtcga gcgaatccga gacgttgtgc aggccgtaga cctggccgaa
     1021 cgacggacgc gccggctcga tgcgggcgac gacgtgcttg cggccctgcc gcgagcgcgt
     1081 caccacgagc gccttgcggc ttcgggacct ggaagcaacc ttggtcgtcg cgtgacgggt
     1141 tgccaccgcg tgccgggctg ccggatgttt cgacttggca ggcggattgt tggccgccac
     1201 cgcagcggct gcgcacgcga ggctcatgaa agccacacca atcagattgc ggatcgtcga
     1261 cacattcatt ccccggggag cagcttcggg cgggagtcta ttcgccgcga gaaaatcgcg
     1321 caagatcaaa aacttgcgcg tcgttcctaa agtgagacta caggcacgta aagttcggtc
     1381 gccagaagac ccgaggcaaa ggaaccagct ccccttcaac cctgggccgc gacgcgctcc
     1441 tgcttgttgt gaagcttgtt caacgccgca agataggctt tcgcggacgc cacgacgatg
     1501 tcgggatcgg cgcccacgcc gttgacgacc cgtcccccat gctgcaagcg caccgtcacc
     1561 tcgccttgcg attctgtgct tccgctggtg atggcattga ccgaatagag cagcatttcc
     1621 gccccactcg cgaccttcga ctcgatcgcc cggaggctgg catcgaccgg cccgttgccg
     1681 tcgctgctcg ccccgtgctc gacgccgccc gcggcgaaaa cgacacgggc atgcggccgc
     1741 gcgccggtct cggaatgctg cgccagcgag atgagccggt agtgatcgtg atcggcaacg
     1801 acggcctcgt ccatgacgag cgccaggatg tcctcgtcga agatgtcgct ctttcggtcg
     1861 gccagctcct tgaagcggac gaacgccgca ttgacgtcgg cctcggactc catctcgatg
     1921 ccgagttcct tcaggcgctg cctgaatgcg ttgcgtcccg acagcttgcc gagaacgatc
     1981 ttgttggccg accagccgac gtcctcggcg cgcatgatct cgtaggtatc gcgcgccttg
     2041 aggacgccgt cctggtggat ccccgaggcg tgcgcgaatg cattcgcgcc gacgactgcc
     2101 ttgttcggct ggacgacgaa gccggtggtc tgcgagacga gccgcgaagc cggaacgatc
     2161 tgcgtcgcgt cgacgccgat ggcgaggtcg aaatagtcct tgcgggtctt caccgccatc
     2221 accacttcct cgagcgagca attgccggcg cgctcgccca ggccgttgat ggtgcattcg
     2281 atctgccggg cgccgccgat cttcacgccg gcgagcgaat tcgccaccgc catgcccagg
     2341 tcgttgtggc aatgcacgga ccagacggcc ttgtccgaat tgggaatccg ctggcgcagg
     2401 tcgcgcagga attcaccgta cagctccgga atggcgtagc cgacggtgtc gggaatgttg
     2461 atggtcgtcg cgccctcggc gatcaccgcc tcgagcacgc gacaaaggaa atccggttcc
     2521 gaccggctgc cgtcctcggg cgagaattcg acgtcggcgc agtgcttgcg cgcgaaacgc
     2581 accgagagct tcgcctgctc gtacacctgc tccggcgtca tgcgcagctt cttctccatg
     2641 tgcagcgcgc tcgtggcgat gaacgtgtgg atgcgccacg gccccttcgc gccctgcagc
     2701 gcctcgatgc cgcgctggat gtcgcggtcg ttcgcccggc acaggccgca caccaccgaa
     2761 tcgcgcacgg tattggcgat cgcgcgcact gcctcgaagt cgccgttgga ggatgcgggg
     2821 aagccggcct cgatcacatc tacgcgcatg cgctcgagcg agcgtgcgat gcgcagcttc
     2881 tcgtcgcgcg tcatcgaggc gccggggctt tgctccccgt cgcgcatggt gg
//
LOCUS       DASRWS010000387         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1301774, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000387 DASRWS010000000
VERSION     DASRWS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301774"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..732)
                     /locus_tag="VFF72_07255"
     CDS             complement(136..732)
                     /locus_tag="VFF72_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HZV92998.1"
                     /translation="MSHADHTSDVDRILDFWFGAPGSDEYASLRKIWFAKDASFDRRI
                     VETFSPLIEKALRGELDDWGTDELSALARILLLDQFTRNAFRGQARAFAGDAQALAAA
                     SAMVGAKLDERLPPFMRAFVYLPFEHAEGMAMQDESVRLFSRLAATSPELGSMLDFAK
                     RHRDVIDTFGRFPHRNAALGRASTAAEVEYLSRPGSGF"
     gene            complement(729..1277)
                     /locus_tag="VFF72_07260"
     CDS             complement(729..1277)
                     /locus_tag="VFF72_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HZV92999.1"
                     /translation="MALKATVCRAALQIADIDRGVYIDRALTLARHPSENDERMMVRL
                     LAFALNVPADPRDGDLEFAKGMWDPDEPEMWERDLTGRIRHWIEVGQPEERRLAKASG
                     RADRVSVYAFSHSARNWWSGLGARLRNAPGVEIWQIAAEQSRELALLAARGMQLQLTR
                     QDGEVLVADGDRSVRVLPERMT"
     gene            1346..1636
                     /locus_tag="VFF72_07265"
     CDS             1346..1636
                     /locus_tag="VFF72_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93000.1"
                     /translation="MSMKKSDLDKQLGKKLGGRMKTAGVPDRFGHAGEEAVDRREQRR
                     RDAAAGLVAFACKLPADLAATLRERAAGHAGGLNGLVEEALRKGLAASGPAE"
     gene            1633..2997
                     /gene="gorA"
                     /locus_tag="VFF72_07270"
     CDS             1633..2997
                     /gene="gorA"
                     /locus_tag="VFF72_07270"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HZV93001.1"
                     /translation="MSDSPFDLFVIGAGSGGVRAARFAAQRGARVAVAEDAALGGTCV
                     NLGCIPKKLYSFAAHYAESFEEAVGFGWSHAAPSFDWTTLKTRRAAEISRLNGIYEGL
                     LASAGVTLLRSRARLVGPHAVEVAGRRVEAERILVATGGWPVPPDFAGGELAISSNEI
                     FDLAGFPARLVVVGGGYIACEFASIFNGLGSQVTQLYRGEQILRGFDDEVRDFTAAEM
                     RKKGVDIRTGAKVDRLERAAAGGPISVRLAGGEVLMADVVLGATGRAPNTAGLGLQTL
                     GVELAPNGAVVVDDAYRSSVDGIYAVGDVIDRVQLTPVALAEAMALVEHLFGAAERRV
                     DYAHIPTAVFTHPNIGTIGFSERDARARFERVRVYRSEFKALRHTLSGSTERTLMKLV
                     VDDANDRVVGLHMVGADAGETIQGFAVAMKAGATKATFDSTLGIHPTAAEEFVTMRTA
                     VVAS"
     gene            3101..3568
                     /locus_tag="VFF72_07275"
     CDS             3101..3568
                     /locus_tag="VFF72_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93002.1"
                     /translation="MTSSPFVASRGVVVAFCLAMLAGAAAAKTGKPQKQPILTPAQLR
                     ACVAQKDDLHAKTDEALKDKNVIGAEKAEIERSAAALGGEVATLDRTSASAVDAYNGK
                     VAERDRRIDAYQAHVDAYNDKAKALQSLQDAYSRACENRRYDENDLPGAKRKK"
     gene            3565..>4456
                     /locus_tag="VFF72_07280"
     CDS             3565..>4456
                     /locus_tag="VFF72_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZV93003.1"
                     /translation="MTTTANPLLHPWNGPFGLPPFAEVRAGHFEPAFDVAMREHLAEV
                     DAIGGAASAPTFDNTIGALDRAGRLFDRVGGLFHNLASSETSPQLQGVERLMAPRLAA
                     HDSRLYMNGPLFERIRSLHDRRADLALSGEQRRVLERYHTDFVRAGARLDAGAQARHA
                     ELMERLADLTTRFGQNVLADESNFRLLLEGEADFAGLPDFVRAATRQAASERGIADAG
                     LVTLSRSHIVPFLTFSERRDLREAAWRAWVTRGEHAGESDNRAIAAEILALRLERARL
                     HGYASYADFVLADTMAASQDA"
BASE COUNT          683 a         1580 c         1540 g          653 t
ORIGIN      
        1 gatcgcgacc acgacggcgt gccgaatcgc tacgaccggc atccgaacaa cccctatcgg
       61 cgctgatcgc aggcgcgcgc gacctcgaca cgagccggaa tagatcggcg cgagacggcg
      121 cgcacgcgcg ccgtctcaaa agccgctgcc cgggcgcgac aggtactcga cctcggccgc
      181 ggtcgaggcc cggccgaggg ccgcgttgcg gtgcggaaag cggccgaagg tgtcgatgac
      241 gtcgcgatgc cgctttgcga agtcgagcat cgacccgagc tcgggtgagg ttgccgccag
      301 cctcgagaac agacgcaccg attcgtcctg catcgccatg ccttcggcgt gctcgaaagg
      361 caggtagacg aaggcgcgca tgaaaggcgg caggcgttcg tcgagtttgg cgccgaccat
      421 ggcgctggcg gcggcgagcg cctgggcgtc gccggcgaat gcgcgtgcct ggccgcggaa
      481 cgcgttgcgc gtgaactgat cgagcaggag gatgcgcgcg agcgcgctca gctcgtcggt
      541 gccccagtcg tcgagctcgc cgcgaagcgc cttctcgatc agcggcgaga acgtctcgac
      601 gatgcgtcga tcgaacgacg cgtccttcgc gaaccagatc ttgcgcaggc tggcgtactc
      661 gtcgcttccg ggtgcgccga accagaagtc gagaatcctg tcgacgtcgc tcgtgtgatc
      721 cgcgtggctc atgtcatccg ctccggcagg acgcgcacgc tgcgatcgcc gtcggcgacg
      781 agaacctcgc cgtcctggcg cgtcagttgc agctgcatgc cgcgcgccgc gaggagtgcc
      841 agctcgcggc tttgctcggc ggcaatctgc cagatctcga cgccgggcgc attcctcagc
      901 ctggcgccca ggccgctcca ccagtttcgc gccgagtggc tgaaggcata gacgctgacg
      961 cgatcggcac gcccgctcgc cttggcgagc cgcctttcct cgggctggcc gacctcgatc
     1021 cagtggcgaa tccggccggt gaggtccctc tcccacattt cgggctcgtc cggatcccac
     1081 atgcccttgg cgaattcgag atcgccgtcg cgtgggtcgg ccgggacgtt cagggcgaat
     1141 gcgagcagcc gaaccatcat ccgctcgtcg ttctcggagg gatgccgggc cagcgtcagg
     1201 gcacggtcga tgtagacgcc gcgatcgatg tcggcgatct gcagcgctgc ccggcacacg
     1261 gttgccttca aagccatcgc ccgactctgc cacaacgccg aggcgacaat gtcggccgta
     1321 ccactgcagc cgcgtgccga gcaccatgag catgaagaaa tccgacctcg acaagcagct
     1381 gggcaagaag ctcggcggcc gcatgaagac cgcgggcgtg cccgatcgct tcggccacgc
     1441 cggcgaggag gccgtcgacc ggcgcgagca gcggcggcgc gacgccgcgg ccgggctggt
     1501 ggccttcgcc tgcaagctgc cggccgacct cgcggcgacc ctgcgagagc gcgccgcggg
     1561 ccacgccggc ggcctcaacg gcctggtcga agaagcactg cgcaaggggc ttgctgcgtc
     1621 gggacccgcc gagtgagcga ttcacccttc gatctcttcg tcatcggtgc cggcagcggc
     1681 ggcgtgcggg ccgcgcgctt cgccgcccag cgcggcgcgc gggtggcggt ggccgaagac
     1741 gcggcactcg gtgggacctg cgtcaacctc ggctgcatcc cgaaaaagct ctacagcttc
     1801 gctgcgcact acgcggagag cttcgaggag gcggtcgggt tcggctggtc gcacgcggcg
     1861 ccgtcgttcg actggacgac gctgaagacc cgccgcgcgg ccgagatctc gcgcctgaac
     1921 ggcatctatg aaggcctgct cgcctcggcc ggagtgacgc tgctgcgcag tcgtgcccgc
     1981 ctcgtcgggc cgcatgccgt cgaggtcgcc ggccggcggg tcgaggccga gcgcatcctc
     2041 gtcgcgacgg gtggctggcc cgtgccgccc gacttcgccg gcggcgaact ggcgatcagc
     2101 tcgaacgaga tcttcgacct cgccggtttt cccgcgcggc tcgtcgtcgt cggcggcggc
     2161 tacatcgcct gcgagttcgc ctcgatcttc aacggcctgg gcagccaggt cacccagctc
     2221 taccgcggcg agcagatcct gcgtggattc gacgacgagg tgcgcgactt caccgccgcc
     2281 gaaatgcgca agaagggcgt cgacatccgc accggcgcga aggtcgacag gctcgagcgc
     2341 gccgcggccg gcggcccgat cagcgttcgc ctcgccggcg gcgaggtgct catggccgat
     2401 gtggtgctcg gcgcgacggg acgggcgccg aacaccgccg ggctcggact gcagacgctg
     2461 ggcgtcgagc tcgcgcccaa cggcgccgtc gtcgtcgacg acgcctatcg cagcagcgtc
     2521 gacggcatct acgccgtcgg cgacgtgatc gaccgcgtcc agctgacccc ggtcgcgctc
     2581 gccgaggcga tggcgctggt cgagcatctg ttcggcgctg ccgagcgccg ggtcgactac
     2641 gcgcacattc cgacggcggt gttcacccat cccaacatcg gcacgatcgg cttttccgag
     2701 cgcgatgcgc gagcccgctt cgagcgcgtc cgcgtctacc gcagcgagtt caaggcgctg
     2761 cgccatacct tgtcgggcag taccgagcgc acgctgatga agctggtcgt cgacgatgcc
     2821 aacgatcgcg tcgtcggcct gcacatggtc ggcgccgacg ccggcgagac gatccagggc
     2881 ttcgctgtcg ccatgaaggc cggcgcgacg aaggcgacct tcgactcgac cctggggatt
     2941 cacccgaccg cggccgagga gttcgtgacg atgcgaacgg cggtcgtggc atcatgaaac
     3001 gcgcgccgac cggcatcgcc cggctgttcg cggcgtctcg ccgcaatgcg tcgccagccc
     3061 ctggcctggc agcgcccacc tctctcaacc cgttcgctcc atgacctcat cacccttcgt
     3121 cgcttcgcgt ggcgttgtcg tcgccttctg tcttgccatg ctcgccggcg ctgccgcagc
     3181 caagacgggc aaaccgcaga agcagcccat cctgacgccg gcgcagttgc gcgcatgcgt
     3241 ggcgcagaag gacgacctgc acgcgaagac cgacgaagcg ctcaaggaca agaacgtgat
     3301 cggcgccgag aaggcggaga tcgagcgaag tgccgccgcg ctcggcggcg aggtcgccac
     3361 gctcgaccgg acgagcgcgt cggcagtcga cgcctacaac ggcaaggtgg ccgaacgcga
     3421 ccgccgcatc gatgcctacc aggcgcatgt cgacgcctac aacgacaagg ccaaggcgct
     3481 gcagtcgctc caggacgcct actcgcgggc ctgcgagaac cgacgctacg acgaaaacga
     3541 cctgccagga gcgaagcgaa agaaatgacg acgaccgcca atcccttgct gcatccgtgg
     3601 aacggaccgt tcgggctgcc gccgttcgcc gaggtccgcg ccgggcattt cgagcctgcc
     3661 ttcgacgtgg cgatgcgcga gcatcttgcc gaggtcgacg cgatcggcgg tgccgcgtcg
     3721 gcgccgacgt tcgacaacac catcggcgcg ctcgatcggg ccggccggct gttcgaccgt
     3781 gtcggcggcc tgttccacaa cctcgcctcg agcgagacct cgccgcagct gcagggcgtc
     3841 gagcgtctga tggcgccgcg cctggccgcc cacgacagcc gtctctacat gaacgggccg
     3901 ctgttcgagc ggatccggtc gctgcacgac cggcgcgccg acctcgcact gagtggcgag
     3961 cagcggcggg tcctcgagcg ctatcacacc gatttcgtcc gcgccggtgc ccgcctcgac
     4021 gcgggcgcgc aggcgcgcca cgccgagctg atggagcggc tggccgacct gacgacgcgc
     4081 ttcggccaga acgtcctcgc cgacgaatcg aacttccggc tgcttcttga gggcgaggcc
     4141 gatttcgccg ggctgcccga tttcgtacgt gccgcgacgc gccaggcggc gagcgagcgc
     4201 ggcatcgccg acgccgggct ggtgacgctg tcgcgttcgc acatcgtgcc gttcctcacc
     4261 ttctccgagc gccgcgacct gcgcgaggcg gcgtggcggg cctgggtgac gcgcggcgag
     4321 cacgcgggcg agagcgacaa ccgcgcgatc gccgccgaga tcctcgccct gcgcctggag
     4381 cgcgcgcgcc tgcacggcta cgcgtcgtat gccgatttcg tgctcgccga caccatggcc
     4441 gcctcgcagg acgcgg
//
LOCUS       DASRWS010000388         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1302061, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000388 DASRWS010000000
VERSION     DASRWS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302061"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /gene="earP"
                     /locus_tag="VFF72_07285"
     CDS             <1..995
                     /gene="earP"
                     /locus_tag="VFF72_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HZV93004.1"
                     /translation="RLSAGLAARGESVRLALDEQESLSWLAPSGAPGVAVGTWREAAV
                     EDADVWVETFGCGMPPQASPSRRRVLVNVEHLSAEPYVDRSHAKPSPRFDADGATLTT
                     WFYYPGFRPANGGLIREPGLLDSRRRFGHGIDWLRARGIEKGAGERCVSLFCYANDAF
                     EACLDALSRSPTLLLLTPGPATEQAILCLGPDLRRGSLRAVRLPAMPQVDFDHLIWTG
                     DLAFVRGEDSLVRAIWSGVPFVWQLYPQDDEARAAKLHAFIDLFLEGAAPELAPLRRL
                     FAIWNGLVPAEGFVSALDAIDMAVWRRRCLVFRERLAARPDLCELLVEFAAQKR"
     gene            1082..1636
                     /gene="efp"
                     /locus_tag="VFF72_07290"
     CDS             1082..1636
                     /gene="efp"
                     /locus_tag="VFF72_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZV93005.1"
                     /translation="MKLAQEIRAGNVIMHGKDPMVVLKTEYSRGGRNSATVRMKLKSL
                     LSNSGTEVVFKADDKMDQVILDKKECTYSYFADPMYAFMDADYNQFEVESENMGDAIQ
                     YLEDAMPVEVVFYDGKAISVELPTTVVREITDTEPAVKGDTSGKVLKNAKLATGMEIS
                     VPLFVQTGDKVEIDTRTHEYKKRV"
     gene            1704..2651
                     /locus_tag="VFF72_07295"
     CDS             1704..2651
                     /locus_tag="VFF72_07295"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HZV93006.1"
                     /translation="MAFDFSHVTVPFRMQPGLRRVAPGSPQLTPAQPGSRHLREKINA
                     LATRAGRVLLSMPDFDAAPVLAALAGESMRHGQPAFTRDGHSRWTAPLLAWTVDGNEV
                     IGSGDAAIGRALAALPATQRPAGLACLAFEEDFAVIDGADTTVRWLAVCLPSRWSPEQ
                     KIGKSFAAVHAPVADSALLIAAAEHLARLVTGGDRWERAVWTISADPHLDQHPARGAS
                     GWPVEADADTVARLARFRHERQTFIPLTGATQAVFTIRVDSEPLADALTTIADAGRLH
                     DALASMSAAVLVYRGLTPVRDRLLDWLARRANGSVATSP"
BASE COUNT          446 a          983 c          907 g          475 t
ORIGIN      
        1 ggcgcctgtc ggccggcctc gcggcccgcg gagaaagcgt gcggctcgcg ctcgacgagc
       61 aggaatctct ctcctggctg gcgccctcag gcgcgcccgg cgtcgcggtc ggcacatggc
      121 gcgaagccgc cgtggaagac gccgacgtct gggtcgagac tttcggctgc ggcatgccgc
      181 cgcaagcgtc gccgtcgcga cgacgtgtcc tggtcaacgt cgagcatctg agtgccgaac
      241 cgtacgtcga ccgttcgcac gcgaagccgt cgccccgttt cgatgccgac ggagcgacgc
      301 tcacgacgtg gttctactac ccgggatttc gtccggccaa cggcgggctg atccgcgagc
      361 cgggattgct cgattcccgg cgccgattcg gccatggcat cgactggctg cgcgcacgcg
      421 gcatcgagaa aggcgccggc gagcgttgcg tgagcctctt ttgctacgcc aacgatgcct
      481 tcgaggcctg cctggacgcc ctctcccgtt cgccgaccct gctgctcctc acgccggggc
      541 cggcgaccga acaggcgatc ttgtgcctcg gccccgacct tcgccgcggc tcgcttcgcg
      601 ccgtccggct gcccgccatg ccccaggtcg atttcgatca cctgatctgg acgggcgatc
      661 tcgctttcgt ccgtggcgag gactcgctgg ttcgcgccat ctggtccggc gtgcccttcg
      721 tctggcagct ctatccgcag gacgacgaag cacgggccgc gaagcttcac gccttcatcg
      781 acctgttcct cgagggcgcc gcgccggaac tggcgccgct gcggcgactc ttcgcgatct
      841 ggaacggcct cgtgccggct gaaggattcg tctcggcgct cgatgcgatc gacatggccg
      901 tctggaggag acgatgcctg gtcttccgcg aaaggctggc cgcgcggccc gacctgtgcg
      961 aattgctggt cgaatttgca gcacagaaac gatagaatcg agggttttgc gccggcaagt
     1021 cctgctgcgc atccagactt tcgtcgtcgt tgccgatgac ggacacaaac catcacagac
     1081 catgaaactc gctcaggaaa ttcgtgccgg caacgtcatc atgcatggca aggatccgat
     1141 ggtcgtgctg aagaccgaat acagccgcgg cggtcgcaat tcggccacgg tgcggatgaa
     1201 gctgaagagc ctgctcagca actcgggcac cgaggtcgtc ttcaaggccg acgacaagat
     1261 ggaccaggtc attctcgaca agaaggaatg cacctactcc tacttcgccg atccgatgta
     1321 cgccttcatg gacgccgact acaaccagtt cgaggtcgag tccgagaaca tgggcgacgc
     1381 gatccagtat ctcgaggacg cgatgccggt cgaggtcgtc ttctacgacg gcaaggcgat
     1441 ctcggtggaa ttgccgacca ccgtggttcg cgaaatcacc gacaccgagc ccgccgtgaa
     1501 gggcgatacc tccggaaagg tcctgaagaa cgccaagctc gcgaccggca tggaaatctc
     1561 ggtgcccctg ttcgttcaga ccggcgacaa ggtcgagatc gacacccgca cgcacgagta
     1621 caagaagcgc gtctagggcg attgccgcgc ctcgccgcac cgggagtgcg ggcggccgat
     1681 cggcggggcg ttcatcatcg gtgatggcct tcgatttcag ccacgtcacc gtcccgttca
     1741 ggatgcagcc cggcctgcgc cgcgtcgcgc ccggatcgcc gcagttgacg cccgcgcagc
     1801 ccgggtcccg gcatctgcgc gaaaagatca acgccctggc gactcgcgcc ggccgggtcc
     1861 ttctctcgat gccggacttc gacgcggcgc ccgtgctggc cgcgctggcc ggcgaatcga
     1921 tgcgtcacgg ccaaccggca ttcacccgcg acggccattc ccgctggacg gcgccgcttc
     1981 ttgcctggac ggtcgacggc aacgaagtca tcggcagcgg tgatgccgcc atcggccgcg
     2041 cgcttgccgc gttgccggca acgcagcgcc cggccggtct cgcctgcctt gccttcgagg
     2101 aagacttcgc cgtcatcgac ggtgccgaca cgaccgtccg ctggctggcg gtgtgcctgc
     2161 cctcgcgctg gtcgcccgag cagaagatcg gcaagtcgtt cgccgccgtc cacgctccgg
     2221 tggccgacag cgccttgctc atcgctgccg cagagcatct cgccaggctc gtgaccggcg
     2281 gcgaccgttg ggaacgcgcg gtctggacga tctcggccga tccgcatctc gaccagcatc
     2341 ccgcgcgagg cgcgtccgga tggcctgtcg aggctgacgc cgacacggtc gccaggctgg
     2401 cgaggtttcg ccatgaaagg cagaccttca tcccgctgac gggcgcgacc caggccgtgt
     2461 tcacgattcg cgtcgacagc gagccgctcg ccgacgcctt gacgacgatc gccgacgccg
     2521 gccggctgca cgacgcgctc gccagcatga gtgcagcggt cctcgtctac cgcgggctga
     2581 cgccggttcg cgaccggcta ctcgactggc tggcacggcg ggcgaacgga tcggttgcca
     2641 cctcgccgtg aggacttcgc cgattcgcgc cgcggacatc gagttcgatg ccgacggcat
     2701 tcccttcgct cccgagttcg ccgacatcta ccacccgcgc cagggagcgc atgcccagtc
     2761 ccggcacgtc tttctttccg gaaacggcct gcccgagcga tggcgcggtc g
//
LOCUS       DASRWS010000389         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3603159, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000389 DASRWS010000000
VERSION     DASRWS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603159"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..991)
                     /locus_tag="VFF72_07300"
     CDS             complement(23..991)
                     /locus_tag="VFF72_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93007.1"
                     /translation="MSAAPIAGWRPRLLQLSASWAIWGVLAALLVAAALLSSAFLEPV
                     YLANVVRQAAPVGIAAIGATVVMILGGVDLSVGAVVSFAAVFAAIQMNGDPARLGPAI
                     AWTLLASALLGLVNGALIAWNRASSFILTLGSALAIYGATQLYSGGTARGIVAPGFRE
                     FFNLRLGDVVPVLALVMVAFALFVEALLRFTRFGRSVYLIGSNAAAARISGLSLARVT
                     IAAYTISGLFAGLAGLALLARSGVSTTSSGRGFELDTLAAIVLGGTVFEGGRGSAIAT
                     VGGVLVLFVAFNIVNILGLNFNLQLTVKGLIVIVASAIYARLNTDR"
     gene            complement(1014..>2017)
                     /locus_tag="VFF72_07305"
     CDS             complement(1014..>2017)
                     /locus_tag="VFF72_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93008.1"
                     /translation="VRLGGEQRRRRRSPRLMPVRALAPVATALDGRAIYLLLAALVAG
                     AALSVPAFRSADNLANVLTQAAALGLVAIGQTFVIVAGLIDLSLGQLLGLVVVLACAL
                     MDGHAERTPAAVVLAVGIGAGIGWLNGRLFNLLRVHSLILTFGTASILQGAIFAYTDR
                     SVGAASPQVLWLANGRVGGVPAALLVLALAAAAGHLVLTRTRFGQHLRAAGGDAESAR
                     RAGVDVARVQRVAFVLSGLGAGLGGILVAGRIGSGYPNAGQAFELDSIVAVVLGGTSL
                     AGGRGSVAGTVAAVLLLAVASNVLNLLEVPTFMQTITKGCIVVVAVLLGQRAAGAQR"
BASE COUNT          365 a          700 c          735 g          217 t
ORIGIN      
        1 gtctccttgg gatcggtggg tttcatcggt cggtgttgag gcgggcgtag atcgccgacg
       61 cgacgatcac gatcaggccc ttgacggtga gctggaggtt gaagttcaag ccgaggatgt
      121 tgacgatatt gaaggcgacg aacaagacca gcacgcctcc caccgtcgcg atggcgctgc
      181 cgcggccgcc ttcgaagacg gtgccaccca ggacgatcgc ggccagcgtg tcgagctcga
      241 agccgcgccc cgagctggtc gtggagacgc ccgagcgggc cagcagcgcc agcccggcca
      301 ggccggcgaa gaggcccgag atcgtgtagg cggcgatcgt cacccgtgcc agcgagaggc
      361 cggagatgcg cgccgccgcc gcattgctgc cgatgaggta gaccgagcgg ccgaaccgcg
      421 tgaagcgaag cagcgcctcg acgaataacg cgaacgccac catcaccagc gcaagtaccg
      481 gcacgacgtc gccgagtcgc aggttgaaga actcgcgaaa gcccggggcg acgatgccgc
      541 gtgcggtgcc gcccgaatag agctgcgtcg cgccgtagat cgccagcgcg ctgcccagtg
      601 tcaggatgaa cgaagaagcg cggttccagg cgatcagggc gccgttgacc aggccgagaa
      661 gcgccgaggc gagcagcgtc caggcgatcg ccggcccaag ccgggccggg tcgccgttca
      721 tctggatcgc ggcaaacacg gcggcgaagg agacgacagc gccgaccgag agatcgacgc
      781 cgcccaggat catcaccacc gtcgcgccga tcgcggcaat gccgaccggc gccgcctggc
      841 gcacgacgtt ggcgaggtag accggctcga ggaaggcgct cgacagcaac gcggcggcga
      901 cgagcagcgc cgccagtacg ccccagatcg cccagctcgc cgagagctgc aacaggcgcg
      961 gacgccagcc ggcgatcggg gccgcgctca tcgtgccgcc acgctcgcgg cgctcatcgt
     1021 tgcgccccgg ccgcgcgttg cccgagcagg acggcgacga cgacgatgca gcccttggtg
     1081 attgtctgca tgaaggtggg cacctccaac aggttgagca cgttcgaggc gaccgcaagc
     1141 agcagcaccg cggcgacggt gccggcgacg ctgcctcggc cgcccgctag cgacgtgccg
     1201 ccgagcacca cggcgacgat cgagtcgagc tcgaaggcct ggccggcgtt gggatagccc
     1261 gagccgatgc ggccggcgac gaggatgccg ccgagcccgg cgcccagtcc cgagagcacg
     1321 aaggcgacgc gctgcacccg cgcgacgtcg acgccggccc ggcgcgcgct ctcggcatcg
     1381 ccaccagcgg cgcgcaggtg ctggccgaag cgcgtgcgcg tcaggacgag atgcccagcc
     1441 gcggccgcca gcgcgagcac gagaagcgcc gccgggacgc cgccgacgcg cccattggcg
     1501 agccacagca cctgcgggga ggcggcgccg acgctgcgat cggtgtaggc gaagatcgcc
     1561 ccttgcagga tgctggcggt gccgaaggtg aggatcagcg agtgcacgcg caggagattg
     1621 aagagacggc cgttcagcca gccgatgccg gcgccgatgc ccaccgccag caccaccgcc
     1681 gccggcgtcc gctccgcatg gccgtccatc agcgcgcagg cgagcacgac gacgaggccg
     1741 agcaattggc cgagcgatag atcgatcagg ccggcgacga tgacgaaggt ctggccgatt
     1801 gcgacgaggc cgagcgcggc cgcctgagtc agcacgttgg cgaggttgtc ggcactgcga
     1861 aacgccggca ccgacagcgc ggcgccggcg accagcgccg ccagcagcag gtagatcgcg
     1921 cgcccgtcga gggccgtcgc gacgggcgcc agcgcgcgga ccggcatcag ccgaggcgat
     1981 cgacgacgtc gtcgctgctc gccacccagc cgaactt
//
LOCUS       DASRWS010000390         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3603233, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000390 DASRWS010000000
VERSION     DASRWS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603233"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFF72_07310"
     CDS             complement(<1..907)
                     /locus_tag="VFF72_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZV93009.1"
                     /translation="MGTAAAIRFEDIRAAERRLRGEIADTPCLHSRTLSAICGCEVFL
                     KFENLQFTASFKERGALNKLAQLSEDERARGVLAVSAGNHAQAVAYHAERMGLHAVIV
                     MPRFASSVKVENTRRFGAEVVLQGDTFDDARVAGLALATERGLTLVHPFDDAAIIAGQ
                     GTIGLEMLAQQPSIDTLVVAIGGGGLIGGIATAARGVQPDVRIVGVQTERFPAAWNAR
                     HGGTREIGKATIADGIGVKSPGELTMPIIEHLVDDVVLVSEDDIEQAMLMLLEIEKTV
                     VEGAGAVGLAALLKHRQRFAGRRCGL"
     gene            1081..1842
                     /locus_tag="VFF72_07315"
     CDS             1081..1842
                     /locus_tag="VFF72_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZV93010.1"
                     /translation="MTFRLALPCAVALATAPGLVLADATVKPDGQFRYALGAGASYSS
                     GNTSAASANLAADGVRATADSKWTFGGKALWARSEGTTTAENLSLGTQYDHDFTPDWF
                     GFGSADYLRDEFANLADRYAGHGGVGRHLIKNDSTTFDISAGVGYTEDRYIDPADIHG
                     ELRGRYGRAEGVVAEESTHKLTQTTSLRQKLSLFPAIGSGGGYRGVFDTGLAVAMTPL
                     LSLTVGLTYRYDSDPGVGFKHGDTLFVTGISMKID"
     gene            1903..>2376
                     /locus_tag="VFF72_07320"
     CDS             1903..>2376
                     /locus_tag="VFF72_07320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZV93011.1"
                     /translation="MLTPQERLKNPALVIVDMQNDFVRVGAALEVPDARKTIPAHMAL
                     IDAFRRGGRPVVYTKFLTFQQPTLLWEWSPQALPETKCCWKGHKRFYPDLGRELECTD
                     VIDELAPEPGDPIIEKYCYGAFHGTPLAQTLQFLGVDSLVVTGTVTQICVEETARQ"
BASE COUNT          370 a          872 c          770 g          364 t
ORIGIN      
        1 tcagcccgca gcggcggccc gcgaagcgct ggcggtgctt gagcagcgcc gcgaggccga
       61 ccgcgccggc accctcgacc accgtcttct cgatctcgag cagcatcagc atcgcctgct
      121 cgatgtcgtc ctcgctcacc aggacgacgt cgtcgaccag gtgctcgatg atcggcatgg
      181 tcagctcgcc cgggctcttc acgccgatgc cgtcggcgat ggtcgccttg ccgatctcgc
      241 gggtgccgcc gtggcgggcg ttccacgccg ccgggaagcg ctcggtctgc acgccgacga
      301 tgcgcacgtc gggctggacg ccgcgcgccg ccgtggcgat gccgccgatc aggccgccgc
      361 caccgatggc gacgacgagc gtgtcgatcg atggctgctg ggccagcatc tcgaggccga
      421 tcgtgccctg gccggcgatg atggcggcgt cgtcgaacgg atggaccagc gtcaggccgc
      481 gctcggtcgc gagcgccagg ccggcgaccc gcgcgtcgtc gaaggtatct ccctgcagca
      541 cgacctcggc gccgaagcgg cgcgtgttct cgaccttcac gctcgaggcg aagcgcggca
      601 tcacgatcac cgcgtgcagc cccattcgct cggcgtgata cgccaccgcc tgcgcatggt
      661 tgccggccga gactgcgagc acgccgcgcg cccgctcgtc ctcgctcagc tgcgccagct
      721 tgttcaaggc accgcgctcc ttgaacgacg cggtgaactg gaggttctcg aacttcagga
      781 agacctcgca gccgcagatc gccgacagcg tgcgcgaatg caggcacggc gtatcggcga
      841 tctcgccgcg caggcggcgc tcggcggcgc ggatgtcttc gaagcggatc gcggcggcag
      901 ttcccatgcc ttcattctcg cgcggggcgg ccgatgccaa gtcgcacttc gcgaaagggt
      961 cgcatccgcc gtgccagcga cctcgccgcg cggtcgctag cctcgccgct ccggtgagcc
     1021 gcctcggcgc gggcgctgcc ggaaccagcc tctttcacga tccaccagga gataccgccc
     1081 atgaccttcc gcctcgccct gccctgcgcc gtcgctctcg ccacggcccc cggcctcgtg
     1141 ctcgccgacg ccaccgtcaa gcccgacggc caattccgct atgcgctggg cgccggcgcg
     1201 agctactcgt cgggcaacac cagcgcggcc agcgccaacc tcgcggccga cggcgttcgc
     1261 gccaccgccg acagcaagtg gaccttcggc ggcaaggcgc tctgggctcg ctcggaagga
     1321 acgacgacgg ccgagaacct gtcgctcggc acccagtacg accacgactt cacgcccgac
     1381 tggttcggct tcggcagcgc cgactacctg cgcgacgagt tcgccaacct ggccgaccgc
     1441 tacgccggac acggcggcgt cggccggcac ctcatcaaga acgacagcac caccttcgac
     1501 atctcggcag gtgtcggcta caccgaggac cgctacatcg atccggccga catccacggc
     1561 gagctgcgcg gccgctacgg tcgcgccgaa ggcgtcgtcg ccgaggaatc gacgcacaaa
     1621 ctgacgcaga cgacctcgct tcgacagaag ctttcgctct ttccggcgat cggctcgggc
     1681 ggcggctacc gcggcgtctt cgacacgggc ctggcggtgg cgatgacgcc gctattgagc
     1741 ctcaccgtcg gcctcacgta tcgctacgac agcgaccccg gcgtcggatt caagcatggc
     1801 gacaccttgt tcgtcaccgg gatctcgatg aagatcgact gaggcctacg cgatgcgctc
     1861 caccgccgct agactcggtg gcagttcgca gcggaggctt ccatgctcac tcctcaggaa
     1921 agactgaaga atccggcgct cgtcattgtc gacatgcaga acgacttcgt ccgcgtcggc
     1981 gcggcgctcg aggtgcccga tgcacgaaag acaattccgg cgcatatggc gctgatcgac
     2041 gcgttccgcc gggggggtcg gcccgtcgtc tacacgaagt tcctcacctt ccagcagccg
     2101 acgctgctct gggagtggtc gccgcaggcg ctgcccgaga ccaagtgctg ctggaagggc
     2161 cacaagcgct tctatcccga cctcggccgc gagctcgagt gcaccgacgt catcgacgag
     2221 ctggcgcccg agcctggcga tccgatcatc gagaagtact gctacggtgc cttccatggc
     2281 acgccgctgg cgcagacgct gcagttcctc ggcgtcgact cgctggtcgt caccggcacg
     2341 gtgacgcaga tctgcgtcga ggagacggcg cgccag
//
LOCUS       DASRWS010000391         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1302687, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000391 DASRWS010000000
VERSION     DASRWS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302687"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /gene="lpxC"
                     /locus_tag="VFF72_07325"
     CDS             complement(<1..754)
                     /gene="lpxC"
                     /locus_tag="VFF72_07325"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZV93012.1"
                     /translation="MLAQRTLKSMTRAVGVGVHGGQRVEMTLRPAPVDSGIVFRRVDL
                     PRSVDIKADAFSVCDTRMATTISAGGDPDGPKVKTIEHLLSACAGLGLDNLGVDITAE
                     EVPILDGSAASFVYLLQSAGIVMQDAPKRFLRVKRPIEVREGEGASLKWARLEPYEGY
                     KLAFEIEFDHPAVSSTGQQFVFEMDPASYKREIARARTFGFTKELDLMRSRGLGLGGS
                     LDNVVVVDEYKVLNSDGLRYGDEFVKHKILDAI"
     gene            complement(907..2127)
                     /gene="ftsZ"
                     /locus_tag="VFF72_07330"
     CDS             complement(907..2127)
                     /gene="ftsZ"
                     /locus_tag="VFF72_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZV93013.1"
                     /translation="MFESHVAYGGENFAQIKVIGVGGGGSNAVNRMIEAKLHGVEFVA
                     VNTDAQALLHSQASFCLRIGDKLTKGLGAGGNPAIGAKAAEETREAIFDVLKGADMVF
                     ITAGMGGGTGTGASPVIAEIAKEVSALTVGVVTKPFDFEGPRRLKAADHGVAELEANV
                     DSLIVILNDKLLDVLGDDVTQDQAFAHANDVLKNAVGGISDIIHIPGLVNVDFEDVKT
                     VMSEPGKAMMGTAIASGPDRANKAAELAVACPLLEGIDLSGARGVLVLIAANRNTFKL
                     SESRNAMNTIRRYAAEEAHVIYGTAYDESLGDQLRVTVIATGLSPARQRVQTPMTVVH
                     SAPVQRTGTDNLPVLSQQVPMAATSPQPHDYSGLTTPSVWRNGRTAAAKVDALASNGM
                     DEIEIPAFLRKQAD"
BASE COUNT          347 a          793 c          783 g          446 t
ORIGIN      
        1 cgatcgcgtc caggatcttg tgcttgacga actcgtcgcc atagcgaagg ccgtcgctgt
       61 tgaggacctt gtattcgtcg acgacgacga cgttgtcgag gctgccgccg aggccgagcc
      121 ctcgtgagcg catcaggtcg agctccttgg tgaagccgaa ggtgcgagcc cgcgcgatct
      181 cgcgcttgta gctggccgga tccatctcga agacgaattg ctggccggtg gaactgacgg
      241 cgggatgatc gaactcgatc tcgaaggcca gcttgtagcc ttcgtagggt tcgaggcggg
      301 cccatttcaa cgacgcaccc tcgccttcgc gaacctcgat cggacgcttg acgcgcagga
      361 aacgcttggg cgcgtcctgc atgacgatgc cggcgctttg cagcaggtag acgaacgacg
      421 ccgccgatcc gtcgaggatc ggcacttctt cggcggtgat gtcgacgccg aggttgtcga
      481 ggccgaggcc ggcgcaggcc gagagcaggt gctcgatcgt cttcaccttc ggtccgtcgg
      541 gatcgccgcc ggccgagatc gtcgtcgcca ttcgcgtgtc gcagaccgaa aaggcatcgg
      601 ccttgatgtc gaccgagcgc ggcaggtcga cgcgacgaaa gacgatgccg gagtcgacgg
      661 gtgccgggcg cagcgtcatc tcgacccgct ggccgccgtg cacgccgacg ccgacggcgc
      721 gggtcatcga cttcaatgtg cgttgcgcca gcatcggtcg attttaggtc gctgcttcgt
      781 cgcggcgttc gatgtcgtcc gcctcgccgc gagcggcccg acgctcgccc gcaggagcgc
      841 cgccgcgtct cgtgcacggc gggtgccttc gggcgcggat cgagcggcgc gcggcgtctg
      901 gcggcatcaa tccgcctgct tgcgcaggaa cgccgggatc tcgatctcgt ccatgccgtt
      961 gctggccagc gcatcgacct tggctgccgc cgtccgaccg ttgcgccaga cgctgggcgt
     1021 cgtcaggccg ctgtagtcgt ggggttgcgg ggaggtcgcg gccatcggca cctgctggct
     1081 cagcaccggc aggttgtcgg tgccggtgcg ctgcaccggt gcgctgtgca cgacggtcat
     1141 cggtgtctgc acccgctgcc tggccgggct gagcccggtg gcgatgaccg tgacgcgcag
     1201 ctggtcgccg agcgattcgt cgtaggcggt gccgtagatg acgtgcgcct cttccgcggc
     1261 gtagcgacgg atcgtgttca tcgcgttgcg gctctcggac agcttgaagg tgttgcgatt
     1321 cgccgcgatc agcaccagca ccccgcgcgc gcccgacagg tcgatgcctt cgagcagcgg
     1381 gcaggccacc gcgagctcgg ccgccttgtt ggcacgatcg ggccccgacg cgatcgccgt
     1441 gcccatcatc gccttcccag gctcgctcat caccgtcttc acgtcctcga agtcgacgtt
     1501 gacgaggccc gggatgtgga tgatgtcgct gatcccgccc acggcgttct tcagcacgtc
     1561 gttggcgtgg gcgaacgcct ggtcctgcgt gacgtcgtcg ccgagcacgt cgagcagctt
     1621 gtcgttgagg atgacgatca gcgagtcgac attggcctcg agctcggcga caccgtggtc
     1681 ggccgccttc aggcggcgcg gcccctcgaa gtcgaacggc ttggtgacga cgccgacggt
     1741 gagcgcgctc acttccttgg cgatctccgc gatcaccggc gaagcgcccg tgcccgtgcc
     1801 gccgcccatc ccggcggtga tgaagaccat gtccgcgccc ttgaggacgt cgaaaatcgc
     1861 ctcgcgcgtc tcctcggcgg ctttcgcgcc gatggcgggg ttgccgcccg cgccgagacc
     1921 cttcgtcagc ttatcgccga ttcgcaggca gaatgatgcc tgcgaatgga ggagcgcttg
     1981 cgcatcggta ttgacggcga cgaactcgac gccgtgcaac ttcgcttcga tcatgcgatt
     2041 gacggcgtta ctccccccgc caccaacccc gatgaccttt atctgggcaa agttttcgcc
     2101 gccatacgcg acgtgagatt caaacatgcc ctcgtccttc ctcttcgcca ctctccgcga
     2161 tgagggtcga tctcccgcac ccacagggtc ctgtgctcat ctcccccgca tggcaaccag
     2221 tatcgctcac ggcaagaact cgcgcaacca tgcttgccag cgagtttgcc cgatccggtt
     2281 gggctggccc gcgaattccg ctgaatttgt tgtgatgaat gatgcgctgc gggcgagcga
     2341 cactgcgctc cgctcccgct gcgcggtct
//
LOCUS       DASRWS010000392         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3342351, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000392 DASRWS010000000
VERSION     DASRWS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342351"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2647)
                     /locus_tag="VFF72_07335"
     CDS             complement(<1..2647)
                     /locus_tag="VFF72_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93014.1"
                     /translation="MPPSRIEPRPRRIAPLGRRLLLLVGAAIVPVALMSGFALHALWT
                     QQKVQIRQSTVDLARALATSLDTELRLTVSALQTMALSPLLDAGDAQRLAQVRTLASQ
                     VLASRPEWRAVILARPSGEAIFATPALPGGELPTLVERDSFMEAVAQRRPTVGSLMIG
                     PSGAAGIPVRVPVLRDGELRYILTAVVQPQAIVRLIGQQRIPDAWVVSVFDAKGERVA
                     RSRDQKRYFRSHASPSLQRLIAKMGSDAEIVGATTTLEGQDVYTAVSRLGVAPWTVAL
                     GEPTEIAGKALRDSGLAYGGGIAFSLGLGLLAAWLIARGIVGPIGRLRRSADALGRGE
                     PLEEAASDVAEIEAVSKALADAARAGRLHEAERQRLLDAERDARAAAEASERRMHLLA
                     GASALLSRSLEEETTLAAIASVIVPDVADVCRIDLLDAAGRLQRKLTHHSDPRRAEEI
                     ARFVARSTSSADTVGSFPWVIATGRSHLGNFDSPESVTATDPVFQEFIRTVGLCAICV
                     VPLVARGRTIGVIAAIQAESHRSFDAEDAALVAELAQRAALALDNGRLYAESESARRH
                     AEAASLAKDEFLAMLGHELRNPLAPIAASLELMDRRPAADAARERRIIGRQVAHLSRL
                     VDDLLDVSRIASGKVELHRERIDLRDVVMRAVEQTQPVMRARASAPGLSLPDRPVEVS
                     GDPLRLIQVVCNLLVNAAKYSPPDRPIRVEVESDVGTARLVVVDEGVGISAELLPRVF
                     DRFVQGAQPLQRAQGGLGLGLAIARNLVDLHGGRIVAESGGAGRGSRFTVTLPLLAGH
                     GSQMAPAAPATAPPHQPARVLIVDDNDDAAQSLAMLLGFEGHDVRVASDAEQGLAMLG
                     DFDAQIAILDIGLPRMDGHALARALR"
     gene            2844..>3391
                     /locus_tag="VFF72_07340"
     CDS             2844..>3391
                     /locus_tag="VFF72_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZV93015.1"
                     /translation="MSVRRLLIACFAASLPVFAAAQDATTTKFVNLRAGPARDYPLVA
                     TLGPGTPLAVQGCTVGYGWCDVIGAGGLRGWVYAGNLAYPYQNSEVPLLGYGATIGLP
                     IITFSIGSYWNDYYRNRPFYRDRDRWSHHRPPPRPVTRPHGPPRPIVRPPSPGPRPPF
                     VRPRPGEAQPPAPRPPVRPPGGA"
BASE COUNT          473 a         1225 c         1231 g          462 t
ORIGIN      
        1 tgcgcagcgc ccgtgccaag gcgtggccgt ccattcgcgg caggccgata tcgagaatgg
       61 cgatctgggc gtcgaagtcg ccaagcatcg ccaggccctg ctccgcgtcg ctcgccacgc
      121 gcacgtcgtg tccttcgaac ccgagcagca tggccagcga ctgcgccgca tcgtcgttgt
      181 cgtcgacgat cagcacgcgt gccggttggt gtggcggcgc ggtcgccggc gcggccggcg
      241 ccatctgcga cccgtggccg gccagaagcg gcagggttac cgtgaaccgg ctgccgcggc
      301 cggcgccgcc gctctcggcg acgatccttc cgccatgcag atcgacgagg ttgcgggcga
      361 tcgccaggcc cagcccgagc ccaccctggg cgcgctgcag cggctgcgcg ccctgcacga
      421 agcggtcgaa gacgcgcggc agcaactcgg ccgagatgcc gacgccctcg tcgacgacca
      481 ccagcctggc ggtgccgacg tcggactcga cctcgacacg gatcggccga tccggcggcg
      541 aatacttggc tgcgttgacg agcaggttgc agaccacctg gatcagccgc agcggatcgc
      601 cgctcacctc gaccggacga tcgggcagcg acagcccggg cgcggaggca cgggcgcgca
      661 tcaccggctg cgtctgctcg acggcgcgca tcaccacgtc gcgcaggtcg atccgctcgc
      721 gatgcagctc gaccttgccc gaggcgattc gcgagacgtc gagcaggtcg tcgacgaggc
      781 gcgacaggtg cgcgacctgc cggccgatga tgcggcgctc gcgggccgcg tcggcagcgg
      841 gccgccggtc catcagctcg agcgatgccg cgatcggtgc cagcgggttg cgaagctcgt
      901 gacccagcat cgccaggaac tcgtccttgg ccaggctcgc cgcttcggca tgccggcgtg
      961 ccgattccga ttcggcgtag aggcgcccgt tgtcgagcgc gagcgctgcc cgttgcgcga
     1021 gctcggccac cagggcagcg tcttcggcgt cgaacgagcg atgcgattcg gcctggatcg
     1081 cggcgatgac gccgatcgtg cgaccgcgcg ccaccagggg gacgacgcag atcgcgcaca
     1141 ggccgaccgt gcggatgaac tcttgaaaca ccggatcggt cgcggtgacc gattcgggcg
     1201 agtcgaagtt cccgaggtgg ctccggccgg tggcgatcac ccacgggaac gagccgacgg
     1261 tgtcggccga cgacgtgctt cgggcgacga agcgcgcgat ctcctcggcg cggcgcgggt
     1321 cgctgtggtg cgtgagcttt cgctgcagcc ttccggcggc gtcgagcagg tcgatgcggc
     1381 agacatcggc cacgtcgggg acgatcaccg acgcgatcgc cgcgagcgtc gtctcctctt
     1441 cgagtgagcg cgagagaagc gcgctggcgc cggccagcag gtgcatccgg cgctccgacg
     1501 cttcggctgc ggcacgggca tcgcgttcgg cgtcgagcag ccgctggcgt tcggcctcgt
     1561 ggagccgccc ggcgcgcgcc gcgtcggcga gcgccttcga taccgcctcg atctcggcga
     1621 catcgctcgc cgcctcctcg agaggctcgc cgcggccgag cgcgtcggcg ctgcggcgca
     1681 gtcgtccgat cggaccgacg atgccgcggg cgatgagcca ggcggcgagc aggccgaggc
     1741 cgagcgagaa ggcgatgccg ccgccgtagg cgagcccgga gtcgcgcagc gccttgccgg
     1801 cgatctcggt cggctcgccg agggcgaccg tccacggcgc cacacccagc cgcgagacgg
     1861 cggtgtagac gtcctggccc tcgagcgtcg tcgtcgcgcc gacgatctcg gcgtcgctgc
     1921 ccatcttggc gatcaggcgc tgcaacgacg gcgacgcgtg gctgcggaag taccttttct
     1981 ggtcgcgcga gcgggcgaca cgctcgcctt tggcatcgaa cacggagacg acccaggcgt
     2041 cggggatgcg ctgctggccg atcagccgga cgatggcctg tggctgcacc accgccgtga
     2101 ggatgtagcg cagctcgccg tcgcgcagca ccggcacgcg caccggaatg ccggccgcgc
     2161 cgctcgggcc gatcatcagc gatcccaccg tggggcgccg ctgcgccacc gcttccatga
     2221 agctgtcgcg ctcgacgagc gtcggcaact cgcctcccgg cagcgctggc gtggcgaaga
     2281 tcgcttcgcc cgagggccgc gccaggatga ccgcgcgcca ctccggccgc gacgcgagca
     2341 cctggctggc cagggtgcgg acttgcgcaa gtcgctgcgc atcgccggcg tcgagcaggg
     2401 ggctcagcgc catcgtctgc agcgccgaga cggtcaggcg cagctcggtg tcgagcgagg
     2461 tcgccagagc ccgcgccaga tcgacggtcg actggcggat ctgcaccttc tgttgcgtcc
     2521 acaacgcgtg cagcgcgaag ccggacatca gcgccaccgg cacgatcgcc gcgccgacga
     2581 gcagcagcag tcgcctgccc agcggggcga tgcgacgggg tcgcggctca atgcgggaag
     2641 gtggcatgcg gctcggtcgg ggaaggctcg ccggtcgaga cgagcctgag tcggcacgga
     2701 gcccggatcg ggcgacgctg cgcattgtgc cggtcattcg tcggtcgtcg cagcggtcgc
     2761 ggcaggggac ggcgggggcg ccgcgaccga aggtgctggt accctcgggg catgccgacc
     2821 agcaacttcg aaaggagcgc gccatgtccg tgcgacgcct gctcatcgcc tgcttcgctg
     2881 ccagcctgcc ggtcttcgcc gcggcgcagg atgcgaccac gaccaagttc gtcaacctgc
     2941 gcgccggtcc ggcccgcgac tatccgctcg tcgccacgct cgggcccgga acgccgctcg
     3001 cggtccaggg ctgcaccgtc ggctacggct ggtgcgacgt gatcggcgcc ggcggcttgc
     3061 gcggctgggt ctacgccggc aatctggcct atccgtacca gaacagcgaa gtgccgctgc
     3121 tgggctacgg cgcgacgatc ggcctgccga tcatcacctt ctcgatcggc tcgtactgga
     3181 acgactacta ccgcaaccgg cccttctacc gcgaccgtga tcgctggtcc catcaccggc
     3241 cgccgccacg gccggtcacg aggccacacg ggccgccgcg gccgatcgtg cggccgccaa
     3301 gcccgggccc ccggccgcca ttcgttcgtc cgcggccggg cgaggcgcag ccgccggcgc
     3361 cgcggccgcc ggtccgtccc ccgggcgggg c
//
LOCUS       DASRWS010000393         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2732505, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000393 DASRWS010000000
VERSION     DASRWS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732505"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFF72_07345"
     CDS             <1..673
                     /locus_tag="VFF72_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZV93016.1"
                     /translation="PVADCHILPQTGMLGSGLVTAVGVAMAHRLARRPEVVVGMLGDG
                     STNTGYFHEGVNLAAVKRAPIVIVIENNQYAISTPISSMALVKNLSDRAAAYGIPGVS
                     VDGTDVVAVYQAVRTALQQAREGHGPTLVELKAYRWGGQTLKDLDRVRPAEEKAAART
                     RCPVEQMKQRLIDAGALTAAAFSAMVQGTRERIARAEAQARTFAPIAVPDEATVLRDY
                     STYAQ"
     gene            688..1701
                     /locus_tag="VFF72_07350"
     CDS             688..1701
                     /locus_tag="VFF72_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZV93017.1"
                     /translation="MKTMTYRQALNDALLIEMERDERVLILGEDVGPHGGARAVTKGL
                     FDRFGSERVIDTPISELAMSGAAVGLAFSGFRPVLENYIGDIIMFMADSLVTSAAKLH
                     FATNGRLRVPMVVRGCDASRPDGGPHQDTLAAWFAHIPGLKVVVPATPADAKGLLASA
                     IRDDNPVIVFEPFQLYDTEGPVPEGEYTVPIGKARVCVAGKDLTLVAVGGSLPHAMAA
                     QERWARQGVSIEVVDPRTLRPLDTATIRDSVRKTGRLVVVHEGWVTYGIGAEIIACVC
                     EGPELLLTAPAERLGTLDTHLPASIALTSAVLPGAARIDVAVEKVMAAAGRRVSKAAD
                     AAL"
     gene            1688..>2597
                     /locus_tag="VFF72_07355"
     CDS             1688..>2597
                     /locus_tag="VFF72_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93018.1"
                     /translation="MPRSEPATRQAQYEFIIVGAGTAGCVLAHRLSADKSHRVLLLEA
                     GPVDRGFWIHVPLGYGKTISNPKVNWCSWSAPVPGIHGRKLYCPRGKCLGGTSSINGM
                     LYVRGQADDFEDWARLGATGWDWAGVLPYFKRMEDHFLGASAFHGAGGPIHVSRIDSP
                     HVLADAFIASAGHCGIPPTDDYNGARQEGASYFQATIRRGERVSAADAYLRPALGRSN
                     LDVVTDALVQRVDFADGRACGVTYSRNGTLCTAYAAREVIVSAGAIGSPQLLQVSGVG
                     AADHLASLGIRVVADLAAVGADFHDHL"
BASE COUNT          422 a          894 c          870 g          411 t
ORIGIN      
        1 cccggtcgcc gattgccaca tcctgccgca gaccggaatg cttggcagcg gcctagtcac
       61 cgccgtcggc gttgccatgg cgcaccgcct ggcgcgccgg ccggaggtgg tcgtcggcat
      121 gctcggcgac ggctcgacga acaccggcta cttccacgag ggggtgaatc tcgcggccgt
      181 caagcgcgcg ccgatcgtga tcgtgatcga gaacaaccag tacgcgatct cgacaccaat
      241 ctcgtcgatg gccctggtga aaaacctctc ggatcgggcc gccgcctatg gcatcccggg
      301 agtcagcgtc gacggaaccg atgtcgtggc cgtgtaccag gcagtccgca cggcactgca
      361 gcaagcgcgc gagggccatg ggccgacgct cgtcgagctg aaggcatatc ggtggggcgg
      421 ccagacgctg aaggatctcg atcgcgtgcg ccctgccgag gagaaggccg cggcgcgcac
      481 ccgctgcccg gtcgagcaga tgaagcagcg tctgatcgac gctggcgcac tcacggcggc
      541 tgcgttctcg gccatggtgc aaggcacgcg agagcggatt gcacgcgccg aggcgcaagc
      601 gcgcacgttc gcacccatcg ccgtgcccga cgaggccacc gtgctccgcg actactcgac
      661 ctacgcccaa tgaatgcagg acatcccatg aagacgatga cgtaccggca agcgctcaac
      721 gacgccctgc tcatcgagat ggagcgtgac gaacgcgttc tcatcctggg cgaggacgtg
      781 ggtccccacg gcggggcccg ggccgtcacg aagggcctct tcgatcgctt cggcagcgaa
      841 cgggtcatcg acacgccgat ctcggagctg gcgatgagcg gcgcggccgt cggcctggcg
      901 ttcagcggct ttcgtcctgt cctcgaaaac tatatcggcg acatcatcat gttcatggcc
      961 gacagcctgg tgaccagtgc agcgaagctg catttcgcca ccaacgggcg gcttcgcgtg
     1021 cccatggtgg tgcgcggctg cgacgccagc cgtcctgacg gcggcccgca ccaggacacc
     1081 ctggccgcct ggttcgcgca catcccgggc ctcaaggtcg tggtcccggc cacgcccgcc
     1141 gacgccaaag gcctcctcgc atcggccatt cgcgacgaca acccggtgat cgtcttcgag
     1201 ccgttccagc tgtacgacac cgaaggaccc gttcccgagg gcgaatacac cgtccccatc
     1261 ggcaaggccc gagtctgcgt cgcgggaaag gacttgacgc tggtagctgt cggcggctcg
     1321 ctcccgcacg cgatggccgc gcaggagcgc tgggcacggc agggcgtgtc gatcgaagtc
     1381 gtcgacccac gaactctgcg gccgctcgat accgcaacga tccgcgattc ggttcgcaag
     1441 accggccggc tcgtcgtcgt tcatgaaggc tgggtcacgt acggcatcgg cgccgagatc
     1501 atcgcctgcg tctgcgaagg accggagttg ctcctcacgg cgccggccga gcgtctcggc
     1561 acgctcgaca cccatcttcc cgccagcatc gccctcacga gcgcggtcct tccgggggct
     1621 gcccgcatcg acgtcgccgt cgaaaaggtg atggccgcgg ccggcaggcg ggtgagcaag
     1681 gcggcagatg ccgcgctctg agcccgcgac acgccaggcg cagtacgaat tcatcatcgt
     1741 cggcgcaggc acggcgggtt gcgtgcttgc ccatcgcctg agcgccgaca agtcgcatcg
     1801 ggtcctcctg ctcgaagcgg gaccggtcga tcgcggcttc tggattcacg taccgctcgg
     1861 ctatggcaag accatctcca atccgaaggt gaactggtgt tcgtggtcgg cgccggtgcc
     1921 cggaatccac ggccgcaagc tctactgccc gcgcggcaag tgcctgggag gcacgagctc
     1981 gatcaacggc atgctgtatg tgcgcggaca ggcagacgac ttcgaggact gggcacgcct
     2041 gggcgccacg ggctgggact gggccggggt gttgccttat ttcaagcgga tggaagatca
     2101 cttcctcggc gcgagcgcgt tccatggcgc gggcggcccg atacacgtgt cgcggatcga
     2161 ttcgccgcac gtcctcgccg acgcattcat cgcctctgcg ggccattgcg gcatcccgcc
     2221 gaccgacgac tacaacggcg ccaggcagga aggcgcgagc tacttccagg cgacgatccg
     2281 ccggggcgag cgcgtcagcg ccgcggacgc ctacctgcgc ccggcgctcg gtcgttcgaa
     2341 tctcgacgtc gtcaccgacg cgctcgtgca gcgggtcgac ttcgccgatg gccgtgcctg
     2401 cggcgtgact tactcgagga atggcacgct gtgtaccgcc tatgcggcac gcgaggtcat
     2461 cgtgagcgca ggtgccatcg ggtcgccgca gctgctgcag gtttccggcg tcggagcggc
     2521 cgaccatctc gcgtcgctcg gcatccgtgt ggtcgcggac cttgcggcag tcggcgccga
     2581 tttccacgac cacctgc
//
LOCUS       DASRWS010000394         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1869964, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000394 DASRWS010000000
VERSION     DASRWS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869964"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFF72_07360"
     CDS             <1..783
                     /locus_tag="VFF72_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZV93019.1"
                     /translation="CMAPLPRAFQWADGSAFVNHVELVRRARNAEMPASFWTDPLMYQ
                     GGSDDFLGPRDDIVAASTEWGIDFEAEVAVITGDIVMGTSPQAALEGIRLVMLANDVS
                     LRNLIPAELAKGFGFFQSKPSTAFSPVAVTVDELGDAWHGGRLHLDLESTWNGKRAGL
                     CNAGDEMTFHFGQLIAHLCKTRNCRAGSIVGSGTVSNKDWSRGYSCIAEKRAIETIEE
                     GSPKTSFMQDGDTIRIEMKDATGASLFGAIEQKVVVPATAGR"
     gene            780..1280
                     /locus_tag="VFF72_07365"
     CDS             780..1280
                     /locus_tag="VFF72_07365"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HZV93020.1"
                     /translation="MSQELPRALKFATIWLVLGALVFVGIEWRAHEAKRTRFDVRGDV
                     VEIHRGDDGHYHWPGSLNGRAVDFLVDTGASGVAIPQSLARELGLEGEGTVRSSTAGG
                     EVIGRVFRADLRLDGGVRADNLRVVALPGLESPLLGMDVLGRLHWQQSDGALRIDLRP
                     AGADRR"
     gene            1351..1998
                     /locus_tag="VFF72_07370"
     CDS             1351..1998
                     /locus_tag="VFF72_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HZV93021.1"
                     /translation="MSDANGFGKLVPGFEFLQNLARNAGAAMPGIGQWVAPTLDPAEL
                     DKRIGELRTVQFWLEQNARMLATTIQALEVQRMTLSTLKTMNVRMGDLKEAMTLKPPA
                     APAGSSTKAASSKERSAGQPATAVDPMQWWGALTNQFTQLATSALKDTAADAAKAAAG
                     SMLKQSMDAAAATFRQATAAPGRVAGAMDAAVGKSAKAARGAKPATKRRTRAAKP"
     gene            2092..>2584
                     /locus_tag="VFF72_07375"
     CDS             2092..>2584
                     /locus_tag="VFF72_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZV93022.1"
                     /translation="MRLTGSEPASHERRRRRADVVAALAAALPAHALLWQDEDTAPYE
                     CDGLTAYREQPLVVALPETEAQVAAVLAICHRLAVPVVARGAGTGLSGGALPNRLGVT
                     LSLAKFNRIVGIDVRARTATVQCGVRNLAISEAAAPHGLFYAPDPSSQIACTIGGNVA
                     ENSG"
BASE COUNT          409 a          915 c          905 g          355 t
ORIGIN      
        1 tgcatggcgc cgctgccgcg cgcattccag tgggccgacg gctcggcctt cgtcaaccac
       61 gtcgagctgg tgcgccgtgc gcgcaatgcc gagatgccgg cgagcttctg gaccgatccg
      121 ctgatgtacc agggcggcag cgacgacttc ctcggcccgc gcgacgacat cgtcgccgcc
      181 agcaccgagt ggggaatcga cttcgaggcc gaagtcgccg tgatcacggg cgacatcgtc
      241 atgggcactt cgccgcaagc cgcgctcgag ggcattcgcc tcgtcatgct cgccaacgac
      301 gtcagcctgc gcaacctcat tcctgccgag ctggccaagg gattcggctt cttccagagc
      361 aagccgtcga ccgccttcag cccggttgcc gtcaccgtcg acgagctcgg cgacgcgtgg
      421 cacggcggcc gcctgcatct cgacctggaa agcacctgga acggaaagcg agctggcctg
      481 tgcaacgccg gcgacgagat gaccttccat ttcggccagc tgatcgccca cctctgcaag
      541 acgcgcaact gccgggccgg ctcgatcgtc ggcagcggca ccgtgagcaa caaggactgg
      601 tcgcgcggct actcgtgcat cgccgagaag cgggcgatcg agacgatcga ggaaggcagc
      661 ccgaagacct ccttcatgca ggacggcgac accatccgca tcgagatgaa ggacgccact
      721 ggggcctcgc tgttcggcgc catcgagcag aaggtggtgg tgccggcgac cgcgggtcga
      781 tgagccagga gttgccgcgg gcgctgaagt tcgcgacgat ctggcttgtt ctcggcgctc
      841 tcgtcttcgt cggcatcgaa tggcgcgccc atgaagcgaa gcggacgcgc ttcgacgttc
      901 gcggcgacgt ggtcgagatc caccgcggcg acgacggcca ctaccactgg cccggctcgc
      961 tgaacggccg cgccgtcgac ttcctggtcg ataccggcgc gagcggcgtc gccatcccgc
     1021 agtcgctcgc gcgcgagctc ggcctcgaag gcgagggcac ggtgcgatcg agcaccgccg
     1081 gcggcgaggt gatcggtcgc gtgttccgcg ccgacctgcg cctcgacggc ggcgtgcgcg
     1141 ccgacaacct gcgcgtcgtc gccctgcccg gcctcgagtc gccgctcctc ggcatggacg
     1201 tgctcgggcg cctgcactgg cagcaaagcg acggtgcctt gcggatcgac cttcgcccgg
     1261 ccggcgccga tcgacgctag tcgcgagggc atcggcgcga gactccgcgc gacaatcgcg
     1321 gctcggcgcc gccggcgacg aggagacacg atgagcgacg cgaatggttt cggcaagctg
     1381 gtccccggct tcgagttcct gcaaaacctc gccaggaacg ccggcgcggc catgcccggc
     1441 atcggccagt gggtcgcgcc gacgctcgac ccggccgagc tcgacaagcg catcggcgag
     1501 ttgcgcaccg tgcagttctg gctcgagcag aacgccagga tgctggcgac gacgatccag
     1561 gcactcgagg tgcagcggat gacgctgtcg acgctgaaga cgatgaacgt gcgcatgggc
     1621 gacctgaaag aggcgatgac gctgaagccc ccggccgcac ctgccggctc gtcgacgaag
     1681 gccgcttcca gcaaggagag gtccgccggc cagccggcga ccgccgtcga cccgatgcag
     1741 tggtggggtg cgctcacgaa ccagttcacg cagctcgcga cgtcggcgct gaaggacacc
     1801 gcagccgacg ccgcgaaggc cgcggcggga agcatgctca agcagtcgat ggatgcggca
     1861 gcggccacct tcaggcaggc caccgccgcg cctggcaggg tggccggggc gatggacgcg
     1921 gccgtcggca agtccgcgaa agcggctcgc ggtgcgaagc cggcgacgaa gcggcgcacg
     1981 cgcgcggcca aaccctgaac gaatccggcg atgcttccgg cgaaggccga acccacggcg
     2041 cccacgcctg attccgcgtc tgccgtcgcg tcttccgatg cgatttccgg cttgcgcctg
     2101 accgggtcgg agccggcgtc gcacgaacgc cgccggcgtc gcgccgacgt cgtcgccgcg
     2161 ctcgccgccg cgctgccggc gcatgccctg ctctggcagg acgaggacac cgcgccgtac
     2221 gaatgcgacg gcctcaccgc gtaccgggag cagccgctcg tcgtcgccct gccggagacc
     2281 gaggcgcagg tcgccgcggt gctcgcgatc tgccatcgcc tcgcggtgcc ggtggtggcg
     2341 cgcggcgccg gcacggggct gtcgggcggc gccttgccga atcggctggg cgtgacgctc
     2401 agcctggcca agttcaaccg gatcgtcggc atcgacgttc gcgcccggac cgcgaccgtg
     2461 caatgcggcg tgcgcaactt ggcgatcagc gaagcggccg cgccgcatgg tctcttctac
     2521 gcccccgacc cgagcagcca gatcgcctgc acgatcggcg gcaacgtcgc cgagaactcg
     2581 ggag
//
LOCUS       DASRWS010000395         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1304165, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000395 DASRWS010000000
VERSION     DASRWS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304165"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFF72_07380"
     CDS             complement(<1..253)
                     /locus_tag="VFF72_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZV93023.1"
                     /translation="MTAQALVLDFGGVVSRSLFETHALSERQLGLAPGTLTWRGPFDP
                     AGDPLWRDMQDDRISERDYWRARAREVGRLVGEDWDRMET"
     gene            complement(261..1505)
                     /locus_tag="VFF72_07385"
     CDS             complement(261..1505)
                     /locus_tag="VFF72_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HZV93024.1"
                     /translation="MKEAPVILLVGTIDTKSDEIAFMRECIERAGGRALIMDVGVLGR
                     GRCSADVANSEVAQAAGLTLQEIATSGDENTSMVLMARGAATLATALHAQERIDGLLA
                     LGGTMGTDLALDVANALPLGVPKVLLSTVAYSHLLPPERIPPDLIMVLWAGGLYGLNS
                     LCCSALSQAAGAVVGACRSARSPDASRPMVGMTSLGTSALTYMKLLKPELERRGYDLA
                     VFHTTGLGGRAFEALAAAGRFAAVMDFSLQELVNHLGGSCVTAGPDRLRGAGRAGVPQ
                     IVAPGATDMIDYPAWGSPPARYAGRNEHVHNRLIASVCVDLAMRREAAGAIAERLGEA
                     TGPTCLLLPAQGIEGWDRPGEPLHDPEGLAALLAELRQRVDAKTRFIEIDAHINDDAF
                     AQTALQVFDGWVAEGHVVPGKH"
     gene            complement(1538..2905)
                     /locus_tag="VFF72_07390"
     CDS             complement(1538..2905)
                     /locus_tag="VFF72_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV93025.1"
                     /translation="MTTDIEQLKRDNARYLFHPMAHPKAMLATRPDIIARGEGCWVWD
                     VDGHKMIDGVAGLWSSNLGHSNKRVRDAIVAQLDELPFYNTFRGTSHPRAIELSARLV
                     KLMAPDDVAAVLFSNGGSDAVEGALKIARQYWKLKGQPERTKFISLKQGYHGVHFGGM
                     SVNGLGNVRRNYEPLLPGCFHVDTPWLYHNPYSQDPAELAKICAGLLEREIQFQGADT
                     VAAFIAEPIQGSGGVIVPPADYWPLVRQVCDRHDVLLIADEVVTGFGRSGALFGTRLW
                     GVKADLWALAKGISSGYVPLGATALGKKVTDAFVADSSGLGTVGHGYTYSGHPVAAAA
                     ALATLDVLEEENVVAHVARVGVHFQNRLAEFEPRFSFVGNVRGQGLMAGIEMVSDKAS
                     RTPVARTSDIPQRVAREAYVRGAMVRISGPNMILSPPLVISMAEVDRLVDVLDASFAA
                     VDASL"
     gene            complement(2964..4406)
                     /locus_tag="VFF72_07395"
     CDS             complement(2964..4406)
                     /locus_tag="VFF72_07395"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZV93026.1"
                     /translation="MNYPNPALHVAGSWLEVASGGQRPVINPADESILGTLPLAGESE
                     LAAAAASSLRGFADWRRRTPLERSAVLRRAAQLMRERSSAMALILTLEEGKPLSEATR
                     EVLLSADIIDFQAEEAKRLYGRTVPPRVAGILSQTVQREPIGPAAAFTAWNFPVNLPA
                     RKIGSALAAGCSVIVKPAEETPGSCMAMVRCFLDAGVPADALNLVCGDPAQVSSFLIA
                     HEAIAKVSFTGSVGVGKHLGELCARHSKSFTPELGGHAPVIVCEDADFEFSLKTSVVA
                     KFRNAGQVCASPIRFYVHRRHFDDWSRRFATSASELRLGCGLEAQTQMGPLAHARRID
                     DMQALVTDALELGARLLCGGTRVDRPGYFFAPTVLSDIPPTCRLAHTEPFGPIATVAP
                     FDDLGDAIAQANASRYGLAAYAFTRDLSTAHRLVAELEAGMVGINHFGVSQPEMPFGG
                     WKDSGHGHEMGAEGLLGYTRLKTAFVGVPC"
     gene            complement(4568..>4857)
                     /locus_tag="VFF72_07400"
     CDS             complement(4568..>4857)
                     /locus_tag="VFF72_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZV93027.1"
                     /translation="ASAFPHKTDDGHGPVPQLKRTLSVDGKPHPYLENLMWPGLVTVA
                     NLPSTVRPIGIGADGLPVGVQIIGPYLHDRTTIRFAALCDEAFGAFQQPPL"
BASE COUNT          793 a         1595 c         1707 g          762 t
ORIGIN      
        1 aggtctccat ccggtcccag tcctcgccga cgaggcggcc gacttcacgg gcgcgcgctc
       61 gccagtagtc gcgttcgctg atgcggtcgt cctgcatgtc gcgccagagc ggatcgccgg
      121 ccggatcgaa ggggccacgc cacgtcagcg tgcccggggc caacccgagc tggcgctccg
      181 acagggcgtg cgtctcgaac agcgagcggc tgacgacacc gccgaaatcg agaacgagag
      241 cttgcgccgt catcgcctgt tcaatgcttg cccgggacca catgtccttc ggcgacccag
      301 ccgtcgaaga cctgcaatgc cgtctgggca aacgcatcgt cgttgatgtg cgcgtcgatc
      361 tcgatgaacc gcgtcttcgc gtcgacgcgc tggcgcaatt cggccagcag cgccgcgagt
      421 ccttcgggat cgtgcaaggg ctcacccggt cggtcccagc cttcgatgcc ttgcgcaggc
      481 agcaggaggc aggtcgggcc cgtggcttcg ccgagccgct cggcgatcgc gccggccgct
      541 tcgcgccgca tcgccaagtc gacgcagacg gaggcgatga gacggttgtg gacatgctcg
      601 ttgcgacctg cgtagcgcgc cggtggcgag ccccacgccg gatagtcgat catgtcggtg
      661 gcgcccggcg ccacgatttg cggcacgcct gcgcgaccgg cgccgcgcaa acgatcgggc
      721 cctgccgtca cgcaagagcc gccgaggtga ttgacgagtt cctgcaggct gaaatccatc
      781 accgccgcga atcggcccgc cgccgcaagc gcctcgaagg cgcggccgcc gaggcccgtg
      841 gtgtgaaaga cggcgaggtc gtagccgcgc cgttcgagct cgggcttgag caacttcatg
      901 taggtgaggg cgctggtgcc gagcgacgtc atgccgacca tcggccgact ggcgtcaggc
      961 gaccgggcgc tgcgacaggc gccgaccacg gcacccgcgg cctgggagag cgccgagcag
     1021 cagaggctgt tcaagccata caagccgcct gcccacagga ccatgatcag gtcaggcggg
     1081 atgcgctcgg ggggcagcag atgcgaatag gcgaccgtcg acagcagcac cttcggcacg
     1141 ccgagcggca gcgcattggc aacgtcgagg gccaggtcgg ttcccatggt gccgccgagc
     1201 gcgagcagac cgtcgatccg ctcttgcgca tgaagcgccg tcgccagggt ggcagcgccg
     1261 cgtgccatca ggaccatcga ggtgttctcg tcgccgctgg tggcgatttc ctgcagcgtc
     1321 aggccggcgg cctgcgcgac ttcgctgttg gcgacatcgg cgctgcacct gccacggccg
     1381 aggacgccga catccatgat cagcgcgcgt cctcccgccc gctcgatgca ttcgcgcatg
     1441 aaggcgatct cgtcgctctt cgtgtcgatc gtgccgacca ggaggatgac cggagcttcc
     1501 ttcacgtgct tgcccttgtc tgccggcgtc gtccggttca cagcgacgcg tcgactgccg
     1561 cgaacgacgc gtcgaggaca tcgacgaggc gatcgacctc cgccatcgag atgaccagcg
     1621 gcggcgacag gatcatgttg ggcccggaga tgcgcaccat cgcccctcgc acataagcct
     1681 cgcgcgccac gcgctgcgga atgtcgctcg tgcgcgcgac cggtgtgcgc gaggccttgt
     1741 cggagaccat ctcgatgccg gccatcaggc cctggccgcg aacgttgccg acgaaactga
     1801 acctgggctc gaactcggcc aggcggttct ggaaatggac gccaacgcgt gcgacatggg
     1861 cgaccacgtt ctcttcctcg aggacgtcca gggtggccag cgcagccgcc gcggcgaccg
     1921 ggtggccgct gtaggtgtag ccgtggccga cggtaccgag cccgctgctg tcggcgacga
     1981 acgcatcggt cacctttttg ccgagtgccg tcgcgccgag cggcacgtag cccgacgaga
     2041 tgcccttggc gagcgcccag aggtcggcct tcacacccca caggcgcgtt ccgaacaagg
     2101 caccgctgcg gccgaacccg gtgacgacct cgtcggcgat cagcagcacg tcgtggcggt
     2161 cgcagacctg gcgcacgagc ggccagtaat cggccggcgg aacaatgaca ccgcccgagc
     2221 cctggatcgg ctcggcgatg aaagcggcca ctgtgtcggc gccctggaac tggatctcgc
     2281 gctcgagcag accggcgcag atcttcgcca gctcggccgg gtcctggctg taagggttgt
     2341 gatagagcca gggcgtgtcg acgtgaaagc agccgggcag caggggctcg tagttgcggc
     2401 ggacgttgcc caggccattg accgacatgc cgccgaagtg cacgccgtga tatccctgct
     2461 tgaggctgat gaacttcgtg cgttcaggct ggcccttcag cttccagtac tggcgggcga
     2521 tcttcagcgc cccctcgacc gcgtcggagc cgccgttgct gaagagcacc gccgccacgt
     2581 cgtcgggcgc catcagcttg acgagccggg ccgacagttc gatggcgcgc ggatggctgg
     2641 tgccgcgaaa cgtgttgtaa aagggcagct cgtcgagctg ggcgacgatg gcgtcgcgca
     2701 cgcgcttgtt gctgtggccg aggttcgagc tccagaggcc tgcgacgccg tcgatcatct
     2761 tgtggccgtc gacgtcccag acccagcaac cctcgccgcg ggcgatgatg tccggccgcg
     2821 tcgccagcat ggccttgggg tgggccatgg ggtgaaacag gtagcgggcg ttgtctcgct
     2881 tgagctgctc gatgtcggtc gtcatcgcgg atcctcggga gcgggtggca cggatcgaac
     2941 ccctgtcggc acttgcggtc ggtctagcac gggacgccga cgaaggccgt cttcaggcgc
     3001 gtgtagccga gcaggccttc ggcgcccatc tcgtggccgt ggccgctgtc cttccagccg
     3061 ccgaagggca tttccggctg cgagacgccg aagtggttga tgccgaccat gccggcttcc
     3121 agctcggcga cgaggcggtg tgccgtcgac aggtctctgg tgaaggcgta ggcggccagg
     3181 ccgtagcgcg aggcgttggc ctgcgcgatc gcgtcgccga ggtcgtcgaa cggggcaacg
     3241 gttgcgatgg ggccgaacgg ctcggtgtgg gcaagccggc acgtcggcgg aatgtcgctc
     3301 agcacagtcg gcgcaaaaaa atagccgggg cgatcgaccc gcgtgccgcc gcaaagcagt
     3361 cgtgcgccga gctcgagcgc atcggtgacc agggcctgca tgtcgtcgat gcggcgagcg
     3421 tgggcgagcg gccccatctg tgtctgcgct tccaggccgc aacccaggcg cagctccgaa
     3481 gcgcttgtgg cgaagcgacg gctccagtca tcgaagtgcc tgcgatgcac atagaagcgg
     3541 atcggcgacg cgcagacctg accggcgttg cggaacttgg ccacgacgga cgtcttcagg
     3601 gagaactcga aatcggcgtc ttcgcagacg atgaccggcg cgtggccgcc gagctcgggt
     3661 gtgaagctct tggaatgacg agcgcagagc tcacccaggt gcttgccgac gcccaccgat
     3721 ccggtgaacg acaccttggc gatggcctcg tgggcgatca ggaacgacga gacctgggcc
     3781 gggtctccgc acaccaggtt gagcgcatcg gccgggacac cggcatcgag aaagcatcgg
     3841 accatcgcca tgcagcttcc cggcgtctct tcggcgggct tgacgatcac gctgcagccg
     3901 gcggccaatg cgctgccgat cttgcgggcc ggcaggttga cggggaaatt ccatgcggtg
     3961 aacgcggcgg caggaccgat cggctcacgc tgcaccgtct ggctgaggat gccggcgacg
     4021 cgcggcggaa cggttcgccc gtagagacgc ttggcctctt cggcctggaa gtcgatgatg
     4081 tcggccgaca gcagcacctc gcgtgtggct tcgctcagcg gcttgccttc ctcgagagtc
     4141 aggatcagcg ccatggcgct cgaccgttcg cgcatcagct gagcggcgcg tcgcaggacg
     4201 gccgaacgtt ccagcggcgt ccgtcgacgc cagtcggcaa aaccgcgcag ggaggatgcc
     4261 gccgcggctg ccagttcgct ttcaccggca agcgggagcg tgccgaggat cgattcatcg
     4321 gcggggttga tcaccggccg ctggcccccg gaggcgactt ccagccagct tcctgcaaca
     4381 tgcagcgccg gattcggata gttcatgggc ctttcgggat atgcggatcg ggatgtgcgc
     4441 ggcgagccgg agcggcacga cagtcaggcg ttcaccacgt gcccgacgtt aaggcgtgag
     4501 aagcgacggc ggctatcgca tatcggcaag acgacgccgc gggaaatcac ctagcccggc
     4561 acgcgcctca gagtggcggc tgctggaagg ccccgaacgc ttcgtcgcaa agcgcggcga
     4621 accggatcgt cgtgcggtcg tgcagatagg ggccgatgat ctgcacgccg accgggagac
     4681 catcggcgcc gatgccgatc gggcggaccg tgctcggcag gttggcgacg gtcaccagcc
     4741 ccggccacat gaggttctcg agatagggat gcggctttcc atcgaccgag agcgtgcgct
     4801 tgagctgcgg caccgggccg tgaccatcgt cggtcttgtg cgggaaagcg gacgcca
//
LOCUS       DASRWS010000396         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1304346, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000396 DASRWS010000000
VERSION     DASRWS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304346"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFF72_07405"
     CDS             <1..351
                     /locus_tag="VFF72_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93028.1"
                     /translation="EETGGLDIISNRATAGKIYRTAAGGEYNTYADAVVLDEPIPEAQ
                     AVTLTDVRAGDLVVFKFNVLHRSCAVSAATKSRFTVQLRFCDLFDQEFLGNKFRPGTV
                     KPDQVDYLRKEAAE"
     gene            348..1148
                     /locus_tag="VFF72_07410"
     CDS             348..1148
                     /locus_tag="VFF72_07410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZV93029.1"
                     /translation="MSVFALTEHDAPLEQMYGVHSEKDKAFWNGLQAIRERYPHSDLH
                     TLTHWPVYTKRILLTRFLAHFELFKMTLDLPGSIVELGVSRGVSFFSFHKFLEIFLPT
                     DTSKKVYGFDSFEGLSDFAKEDGETAYDDINDKKGGGWSAKTVEGEIFELCKLTNADN
                     ILARERSRLIKGRVQDTLEPFLKDTPGLRLNLLHFDLDLYEPTLYCLERLWDLIVPGG
                     VVVFDEYGLPPWGGEATAFDEFRQDRGLKVALRKFPWCLTPTAYCVKD"
     gene            1176..>2158
                     /locus_tag="VFF72_07415"
     CDS             1176..>2158
                     /locus_tag="VFF72_07415"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZV93030.1"
                     /translation="MPATAPSDDDAWSFAHDIADVVARCVPVWNELRGASLLITGGTG
                     FIGRWLLASLAAANDRHTLGVTATILTRDPERFRQRAPELASHPAFRLAAGDVRDVAV
                     PAGDFSHVIHGAAEASAAVNEADPARMFDTIVAGTRHVLDLAVARRPKRVLFLSSGAV
                     YGRQPWEMEHVAEDFTGAPDLTDPRATYAEAKRAAEMLCTIYGKQFGLTIAVARIFTL
                     LGPYLPLDTHFAVGNFIRDAMSGRPVTVNSDGRACRSYLYGSDLAVWLWHMLVRAEPG
                     RPYNVGSNESVSIRDLAACVSRIVGSGGVDIRGKADTGWNPGRYVPDTDLIA"
BASE COUNT          367 a          753 c          671 g          367 t
ORIGIN      
        1 gaggagaccg gcggcctcga catcatctcg aaccgcgcca ccgcgggcaa aatctaccgg
       61 acggcggcgg gcggcgaata caacacctac gccgacgccg tcgtgctcga cgagccgatc
      121 ccggaagcgc aggcggtgac gctcaccgac gtgcgcgccg gcgatctggt cgtcttcaag
      181 ttcaacgtgc tgcaccgctc gtgcgcggtg agcgccgcga cgaaatcgcg cttcaccgtg
      241 caactgcggt tctgcgacct gttcgaccag gaattcctcg gcaacaagtt ccggccaggc
      301 acggtgaagc cggatcaggt cgattacctg cgtaaagagg cggcggaatg agcgtctttg
      361 cgttgaccga acacgacgct ccgctcgagc agatgtacgg cgtccattcc gaaaaggaca
      421 aagcgttctg gaacggcctg caggcgatcc gcgagcgcta tccgcactcc gacctgcata
      481 cgctcacgca ctggccagtc tataccaagc gcatcctgct gacgcgcttc ctcgctcatt
      541 tcgagctgtt caagatgacg ctcgatctgc ccggttcgat cgtcgagctc ggcgtctcgc
      601 gcggcgtgtc gttcttctcg tttcacaaat tcctggagat cttcctgccg accgacacgt
      661 cgaagaaggt ctacggcttc gactcgttcg aaggcctgag cgactttgcc aaggaagatg
      721 gcgagaccgc ttatgacgac atcaacgaca agaagggcgg cggctggtcg gccaagaccg
      781 tcgagggcga gatcttcgag ctgtgcaagc tcaccaacgc cgacaacatc ctggcgcgcg
      841 agcgctcgcg gctcatcaaa ggccgggtgc aggacacgct ggagccgttc ctcaaggaca
      901 cgcccggcct acgcctcaac ctgctgcact tcgatctcga tctctacgag ccgacgctct
      961 attgcctcga gcggctgtgg gacctgatcg tgccgggcgg cgtcgtcgtg ttcgacgaat
     1021 acgggctgcc gccgtggggc ggcgaggcca ccgccttcga cgagttcagg caggaccgcg
     1081 ggctgaaagt cgccttgcgc aaattcccct ggtgcctgac gccgaccgcc tattgcgtga
     1141 aggactgatg ccgccgcgac ccggaaggcg accgaatgcc ggcgaccgcg ccctctgacg
     1201 acgacgcctg gtccttcgcc cacgacatcg ccgatgtcgt cgcacgctgc gtcccggtgt
     1261 ggaacgaact gcgcggcgcc agcctgctga tcaccggcgg caccggattc atcggccgct
     1321 ggctgctcgc cagcctcgcc gccgccaacg accggcacac gctcggcgtc acggcgacga
     1381 tcctgacgcg cgatccggag cgctttcggc aaagggcgcc ggagcttgcc tctcatccgg
     1441 catttcgtct ggctgccggc gacgtccgcg atgtcgcagt gccggcgggc gacttcagcc
     1501 acgtcattca cggcgcggcc gaggccagcg cggccgtcaa tgaggccgat ccggcgcgca
     1561 tgttcgacac catcgtcgcc ggcacgcgcc atgtgctcga tctcgccgtg gcgcggcggc
     1621 ccaagcgcgt cctgttcctg agctcgggcg cggtctacgg ccggcagccc tgggagatgg
     1681 agcacgtcgc cgaggatttc accggcgcgc cggatctcac cgacccgcgc gcgacctatg
     1741 ccgaggccaa gcgcgccgcc gaaatgctgt gcacgatcta cggcaaacag ttcggcctca
     1801 ccatcgcggt ggcccgtatc ttcaccctgc tcggcccgta tcttccgctc gacacccact
     1861 tcgctgtcgg caacttcatc cgcgacgcca tgtccggcag accggtgacc gtcaacagcg
     1921 acggccgcgc ctgtcgctcc tatctctacg gctccgacct cgccgtctgg ctctggcaca
     1981 tgctcgtgcg cgccgagcct ggcaggccgt ataatgtcgg ctcgaacgaa tcagtatcga
     2041 ttcgcgacct cgctgcgtgc gtttcgcgca tcgtcgggag cggcggcgtc gacatccggg
     2101 gcaaagccga caccggctgg aatccgggcc gttacgtgcc cgacacggac ctgatcgc
//
LOCUS       DASRWS010000397         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3344072, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000397 DASRWS010000000
VERSION     DASRWS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344072"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /gene="atpE"
                     /locus_tag="VFF72_07420"
     CDS             <1..213
                     /gene="atpE"
                     /locus_tag="VFF72_07420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZV93031.1"
                     /translation="WAFGTAIGFGLLGGRFLEGMARQPELAPALLGRAFIFVALLDAV
                     TFIGIGFALFFTFANPFVAALQAAGH"
     gene            272..742
                     /locus_tag="VFF72_07425"
     CDS             272..742
                     /locus_tag="VFF72_07425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZV93032.1"
                     /translation="MNINLTLVIQMLVFATLVWFTMKYVWPMILGPMEERSRKIAQGL
                     AAGQEGEKSLAEAREKSEAIVREARERATQIIDQAQHRANDLVEQAKGTATAEGQRLV
                     AAAHQQIELDATHARESLRREVAQIAVKAASKLLEREIDPRAHADLIDKLATQI"
     gene            828..1379
                     /locus_tag="VFF72_07430"
     CDS             828..1379
                     /locus_tag="VFF72_07430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZV93033.1"
                     /translation="MADKTTIARPYAKAAFEQASDDKRLTEWSDALRTGAAVVKDARV
                     ENLLGNPQVTPSQMAGLVFDVAGSKLDGDGPGRVSRNFFDMLAENRRLAYLPEIASLF
                     DELKDKAEGVIDVTVTSAAPLDAGQQKTLSSALERKLKRSVRLHCETDPKLIGGAVVR
                     AGDLVIDGSVLAKLNRIAYELTA"
     gene            1406..>2233
                     /locus_tag="VFF72_07435"
     CDS             1406..>2233
                     /locus_tag="VFF72_07435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZV93034.1"
                     /translation="MAIKATEISDLIKQRIENFKSVTEARNVGTIVSVTDGITRIHGL
                     ADAKYGEMLEFPGNTFGLALNLEQDSVGAVVLGDYKHLSEGDTVKTTNRILEVPVGPE
                     LLGRVVDALGQPLDGKGPIAAKRTAPIERVAPGVIYRQSVSQPVQTGYKSVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDTIINQKTSGIRCVYVAIGQKQSTIANVVRKLEEHDAL
                     KHTIIVSASASTPAAMQYIAAYSGVTMGEYFMDNGEDALIIYDDLSKQAVAYRQISLL
                     "
BASE COUNT          439 a          716 c          703 g          375 t
ORIGIN      
        1 tgggcgttcg gcaccgccat cgggttcggc ctgctcggcg gccgcttcct ggagggcatg
       61 gcccgccagc cggagctcgc gccggcgctg ctcggtcgcg cgttcatctt cgtcgcgctt
      121 ctggacgccg tcacgttcat cggcatcggc ttcgctctgt tcttcacttt cgcgaatccg
      181 ttcgttgccg cgttgcaggc ggcggggcac tgaaacgagc ccgcagccac gctgcaggcc
      241 cggtatcaga gccccaagtt aggagacgag agtgaacatc aatctgacgc tcgtcattca
      301 gatgctggtc ttcgcgaccc tcgtctggtt cacgatgaaa tacgtgtggc cgatgattct
      361 cggccccatg gaagagcgtt cgcgcaagat cgcgcagggt ctcgctgccg gccaggaagg
      421 cgagaagtcg cttgccgagg cgcgcgagaa gtctgaggcg atcgttcgtg aagcccgcga
      481 gcgcgccacg cagatcatcg accaggcaca gcaccgcgcc aacgacctcg tcgagcaggc
      541 caagggcact gcgacggccg aaggtcagcg actcgtggct gccgcgcatc agcagatcga
      601 gctggacgcg acccatgctc gcgagagcct gcgccgcgaa gtggcgcaga tcgcggtgaa
      661 ggcggcctcg aagcttcttg agcgtgagat cgatccgcgt gcgcacgccg atctcatcga
      721 caagctggcg acgcagatat gagcggccaa aaaccgcgct ttttggccgc gggcagaaag
      781 cgccgcaggc gactttctgc aatcggacaa taacgcagga gcgcatcttg gcggacaaga
      841 cgaccatcgc acgaccctac gccaaagcgg cgttcgagca agccagcgac gacaagcgac
      901 tgaccgagtg gtctgacgct ttgaggactg gcgcggccgt cgtgaaggat gcgcgcgtgg
      961 agaacctgct cggtaaccca caggtgacac cttcgcaaat ggcaggactg gtgttcgatg
     1021 tggcggggtc gaagctcgac ggcgacggcc cggggcgcgt cagccgcaat ttttttgaca
     1081 tgctcgcgga gaaccggcgt ctggcctacc ttcccgagat cgcgtcgctc ttcgacgagc
     1141 tcaaggacaa ggctgagggc gtgatcgacg tcaccgtgac atccgcagcg ccgctcgatg
     1201 ccggacagca gaagacactc tcgagcgcgc tcgagcgcaa actcaaacgc tccgtgcgct
     1261 tgcattgcga gaccgacccg aagctcatcg ggggcgccgt ggtgcgcgcg ggcgatctcg
     1321 tcatcgacgg ctcggtgctg gccaagctca atcgcatcgc gtacgagctc acggcctgaa
     1381 cgacattcgc tagaggattg acacaatggc catcaaggca accgagatca gcgacctcat
     1441 caaacagcgg atcgagaact tcaaatcggt cactgaggct cgcaacgtcg gcacgatcgt
     1501 ttcggtgacc gacggcatca cccgcatcca cgggctggcg gacgccaagt acggtgagat
     1561 gctggagttt ccgggcaaca ccttcggcct ggcgctcaac ctggagcagg actccgtggg
     1621 cgcggtcgtt ctcggcgact acaagcatct gtcggaaggc gacacggtca agacgaccaa
     1681 ccgcattctc gaggtgccgg tcggtcccga attgctgggc cgcgtggtcg atgcgcttgg
     1741 acagccgctc gatggcaagg gtccgatcgc cgcgaagcgc accgccccca tcgagcgcgt
     1801 ggcgccgggc gtgatctatc gtcagtccgt gagccagcct gtgcagaccg gctacaagtc
     1861 ggtcgactcg atggtcccga tcggtcgcgg tcagcgcgag ctgatcatcg gcgatcgcca
     1921 gacaggcaaa accgcactcg ctatcgacac gatcatcaac caaaagacct ccggcatccg
     1981 ttgcgtttac gtcgccatcg gccagaagca atcgacgatc gccaacgtcg tcagaaagct
     2041 cgaggagcac gatgccctca agcacacgat catcgtgtcg gcctcggcct cgacccccgc
     2101 cgccatgcag tacatcgccg cctactcggg cgtcacgatg ggcgagtact tcatggataa
     2161 cggcgaggac gcactcatta tttacgacga cctgtccaag caggccgtcg cctatcgcca
     2221 gatctcgctg ctg
//
LOCUS       DASRWS010000398         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1609724, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000398 DASRWS010000000
VERSION     DASRWS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609724"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFF72_07440"
     CDS             <1..939
                     /locus_tag="VFF72_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HZV93035.1"
                     /translation="AVLGVATQIWFLIDSAPLGIFWRGVAIGVNYAVWFIVIQHFHGI
                     VATKQPATTAPALADKLGDLSSDAGVERFVDEVAHLIRSQAAGIFGNLLGVVPVVLLA
                     QWLTRQLLGSPLIGTAPALHTLHDLTLLGPTAFYAAITGVVLFASSMIAGWVENWFVW
                     HRLDSAIAWNPRFVARLGATRARRWSVYWRTHIASYAANVSLGLLLGLVPAVALFFSL
                     PVDVRHVTLGTGELFTAVGALGSSTLATPPFWWCVAGVAATGTLNLGVSFLLAFRVAL
                     RSRDIRIADRGRIHRALRRRLRQAPLSFVFPTRPAS"
     gene            complement(1006..2253)
                     /locus_tag="VFF72_07445"
     CDS             complement(1006..2253)
                     /locus_tag="VFF72_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HZV93036.1"
                     /translation="MTIDHIAAFIFALALAHTFAAKLFEHLAHRHPRHAGLLHLLGEV
                     EVVFGFWAVVLIAVMALVSGGTAAIDYAESRQYTEPLFVFTIMVVAASRPMLEAIRSL
                     VAAVARFVPLRTALVEAWLCFALVPLLGSLVTEPAAMTLAALMLGPRLFRPDVPERLK
                     YAALGTLFVNVSIGGTLTSFAAPPVLIVAGTWQWDSAFMASTFGWKAAIAVLVNATGA
                     VALLRSHLPDAAPSQADTGIATPWLVTAIHLVFLVAVVLFAHHPVIFLGLFLLFLGFT
                     QAYGRYQNPLLLKEALLVGYFLAGLVVLGGLQQWWLQPIVSSLRPLALFFGALGLTAI
                     TDNAALTYLGSLISGMSDPAKYMLMAGAVSGGGLTVIANAPNPAGVALLRPGFEDGSI
                     GALGLLAGALLPTVVAAAAFMLL"
     gene            2301..3497
                     /locus_tag="VFF72_07450"
     CDS             2301..3497
                     /locus_tag="VFF72_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HZV93037.1"
                     /translation="MTAPLHCARGIAGAIFVAVSALLAGCSNTATVAPSSPGAILPAG
                     PAATTGNPVAPSPTTPAVRLRSGARWVQADWADLPGWADDRTNRVLPALLASCGHPAP
                     AWAATCNDLQQHVLGLVGNDDATRGWLRHALQPWRVESLQADPVGLATGYFEPLVEAS
                     RHARPGFRVPLYAPPPDLAARRPYWTRRQVDTLPAARRSLRGSELAWLRDPLDALLLQ
                     VQGSGRLIFVDARGRPQETVRVAFAGHNEQPYRSVGRWLVDQGELTLEQASWPAIRAW
                     AARHPDRVDEMLWSNPRLVFFHEEALADPTIGPKGAQGVPLTPGRSIAVDPQSIPYGT
                     PVWLDTTEPLSAAPLRRLVVAQDTGSAITGAVRADYFWGWGDDAEASAGRMKQPLRMW
                     ALWPSS"
BASE COUNT          538 a         1310 c         1274 g          522 t
ORIGIN      
        1 gccgtgctcg gcgtggcgac gcagatctgg ttcctgatcg actcggcgcc gctcggcatc
       61 ttctggcgcg gcgtggcgat cggcgtcaac tacgccgtgt ggttcatcgt catccagcac
      121 ttccacggca tcgtcgccac caagcagccg gcgacgacgg cgccggcgct ggccgacaag
      181 ctgggcgacc tgtccagcga cgccggcgtc gagcgcttcg tcgatgaagt ggcgcacctg
      241 atccggtcgc aagccgccgg catcttcggc aacctgctgg gcgtggtccc ggtcgtgctg
      301 ctggcgcaat ggctgacgcg gcaactgctc ggatcaccct tgatcggaac ggcccccgcg
      361 ctgcacacgc tgcacgacct cacattgctc ggaccgaccg cgttctacgc ggcgatcacc
      421 ggtgtcgtcc tgttcgccag ctcgatgatc gccggctggg tcgagaactg gttcgtctgg
      481 caccggctcg acagcgccat cgcctggaac cctcgcttcg tcgcccgcct gggcgccacg
      541 cgcgctcgcc gctggtcggt gtactggcgc acccacatcg cgagctacgc ggccaacgtg
      601 tcgctcggcc tgctgctcgg actggtgccc gccgtggccc tgttcttcag cctgcccgtc
      661 gacgtgcgcc atgtgacgct cggcaccggc gagctcttca ccgccgtcgg cgcgctcggg
      721 tcgtcgacgc tcgccacccc gccgttctgg tggtgcgtcg ccggcgtcgc ggccaccggc
      781 acgctcaatc tcggcgtgag cttcctgctc gcctttcgcg tcgcccttcg ctcgcgcgac
      841 atccgcatcg ccgatcgcgg ccgcatccat cgcgcgcttc gcaggcggct tcgacaggcg
      901 cccttgtcgt tcgtgttccc gacccggccc gcgtcgtgag gaaacacgtc gccacgcggc
      961 atcaacgccc ggacgagcag gaagacggcc cgcgtcgtcc ggttgtcaga gcagcatgaa
     1021 cgcggcggcg gcgacgacgg tcggcagcag ggcgccggcc agcaggccga gcgcgccgat
     1081 cgagccgtcc tcgaagcccg ggcgcagcaa cgccacgccg gccgggttcg gcgcgttggc
     1141 gatgacggtc aggccgccgc cggagaccgc gccggccatc agcatgtact tggccggatc
     1201 gctcattccg ctgatcagcg acccgagata ggtgagcgcg gcgttgtcgg tgatggcggt
     1261 gaggccgagc gcgccgaaga agagcgcgag cgggcgcagg ctcgagacga tgggctgcaa
     1321 ccaccattgc tgcaggccgc cgaggacgac gagcccggcg aggaaatagc cgacgagcag
     1381 cgcttccttc aggagcaacg ggttctggta gcgcccgtag gcctgggtga agccgagaaa
     1441 cagcaggaag aggccgagaa agatcaccgg gtgatgcgcg aagagcacca ccgcgacgag
     1501 aaagacgaga tggatcgcgg tgaccagcca aggcgtcgcg atccctgtgt cggcttgcga
     1561 cggcgctgcg tcgggcagat gcgagcgcag caaggcgacg gcgccggtgg cgttgacgag
     1621 caccgcgatc gccgccttcc agccgaaggt cgaggccatg aacgcgctgt cccactgcca
     1681 ggtgccggcg acgatcagca ccggcggcgc agcgaacgac gtcagcgtgc cgccgatcga
     1741 gacattgacg aacagcgtgc cgagcgcggc gtacttgagc cgctcgggaa cgtcggggcg
     1801 gaacagtcgg gggccgagca tgagcgcggc gagcgtcatc gccgccggct cggtgacgag
     1861 cgagcccagc aggggcacca gcgcgaagca cagccacgcc tcgaccagtg ccgtgcgcaa
     1921 cggcacgaag cgggcgacgg cggcgaccag cgagcggatc gcctcgagca tcggccgcga
     1981 cgccgcgacc accatgatcg tgaagacgaa gagcggctcg gtgtactggc gcgactcggc
     2041 gtagtcgatc gccgccgttc cacccgagac gagcgccatg acggcgatga gcacgaccgc
     2101 ccagaagccg aacaccacct cgacttcgcc gagcaggtga agcagcccgg catggcgcgg
     2161 atgccggtgc gcgaggtgct cgaaaagttt cgccgcgaag gtgtgcgcca gcgccagcgc
     2221 gaagatgaag gcggcgatgt gatcgatcgt catggccgtc gattctcccc ggcaggtgcg
     2281 ttcttgcaga atcgacgcga atgacggctc ccctgcactg cgcgcgcggc atcgcgggcg
     2341 cgatcttcgt cgccgtgtcc gccttgctgg ccggctgctc gaataccgcg acggtcgcgc
     2401 cctcctcgcc tggggcgatc ctgccggcag ggccggccgc gacgacgggc aatccggtcg
     2461 ctccgtcgcc gacgacgcca gcggttcggc ttcggtcggg cgctcgctgg gtccaggccg
     2521 attgggccga cctccccggc tgggccgacg acaggacgaa tcgggtgctg ccggcgctgc
     2581 tcgccagctg cggccatccc gcgccggcct gggccgccac ctgcaacgac ttgcagcagc
     2641 acgtcctcgg cctcgtcggc aacgacgacg cgacgcgcgg ctggctgcgc cacgcgctgc
     2701 agccgtggcg ggtcgagtcg ctgcaggccg atccggtcgg cctggcgacc ggctatttcg
     2761 aaccgctggt cgaggcatcc cgacatgccc ggcccggctt tcgcgttccc ctctacgcgc
     2821 cgccgcccga cctggccgct cgccgaccct actggacgcg acgccaggtc gacacccttc
     2881 cggccgcgcg tcgatcgctt cgcggcagcg agctcgcctg gctgcgcgac ccgctcgatg
     2941 cgttgctgct gcaggtgcag ggttcgggcc ggctgatctt cgtcgacgcg cgcggccgcc
     3001 cgcaggaaac ggtgcgcgtc gccttcgccg gccacaacga acagccgtat cgatcggtcg
     3061 gccgctggct cgtcgaccag ggcgagctga cgctggagca ggcgtcctgg ccggcgatac
     3121 gcgcctgggc agcgcgccat cccgatcggg tcgacgaaat gctttggagc aatccgcggc
     3181 tggtcttctt ccacgaggag gcgcttgccg atccgacgat cggcccgaag ggggcgcagg
     3241 gcgtgccgct gacgccgggc cgctcgatcg ccgtcgatcc gcagagcatc ccctacggca
     3301 cgccggtctg gctcgacacc accgagccgc tgtcggcggc cccattgcgc cgcctcgtcg
     3361 tcgcccagga caccggctcg gcgatcaccg gcgccgtgcg cgccgactac ttctggggct
     3421 ggggcgacga cgccgaagcg tccgcgggaa ggatgaagca gccgctgcgc atgtgggcgc
     3481 tctggccgtc atcgtgactg cgacgagcgc atcggatccg gcggcgccgc gcgaccgcgt
     3541 cgccatcgac ggcggccggc cgatcgactg gagcctgacg tcgctcgact acgcgaccca
     3601 tcggcccggc ccgccgcagc gtctctacga attgctcgcc gccc
//
LOCUS       DASRWS010000399         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_159976, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000399 DASRWS010000000
VERSION     DASRWS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159976"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFF72_07455"
     CDS             complement(<1..503)
                     /locus_tag="VFF72_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV93038.1"
                     /translation="MDTPALRPLGRSGLHVSPICLGGNVFGWTVDEATSFRLLDAWVD
                     AGMNFVDTADVYSKWVHGHAGGESETIIGKWLKKSGKRNRVVLATKVGKPMGEGRSGL
                     SRAYIRDAVEASLRRLQTDVIDLYQAHEDDRSTPLEESLAAFGELIAEGKVRAIGASN
                     YTADRLS"
     gene            562..2544
                     /locus_tag="VFF72_07460"
     CDS             562..2544
                     /locus_tag="VFF72_07460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HZV93039.1"
                     /translation="MASKQSTYGEASIRVLKGLEPVKQRPGMYTRTESPLHVIQEVID
                     NAADEALGGHATTIDVVLHGDGSISVDDDGRGIPFGVHPDEGVSVVEIVFTRLHAGGK
                     FDKGGGAAYSFSGGLHGVGVSVTNALARRLEVKVWRGGQVASIAFAGGDVAVPLEVRK
                     ATASDRKSGTSIRVWPDPRYFENVELPRPELTHLLRSKAVLMPGLAVSLAIEKAGRAD
                     DERQQWLYKSGLADYLMQALPADPVIPLFEGAHHADGGESEDVAEGEGAAWCVAFTEE
                     GASVRESYVNLIPTVAGGTHESGLKEGLFVAVKGFIDMHGLLPKGVKLMADDVFSRAS
                     FVLSAKVLDPQFQGQIKERLNSRDALRLVSNCVRPALELWLNQHVDLGKKLAEIVIRQ
                     AQTRQRASQKVEKKKGSGVAVLPGKLTDCESRDPARSEIFLVEGDSAGGSAKMGRDKE
                     TQAILPLRGKVLNAWEVERDRLFANNEIHDIAVALGVDPHGAGDDPDLSGLRYGKVCI
                     LSDADVDGSHIQVLLLTLFFRHFPKLIERGHVHVAKPPLFRVDAPARGKKPAAKIYAL
                     DDRELQAVVDRLKKEGARDGSWTISRFKGLGEMNAEQLWDTTLNPETRRLLQVSFGAA
                     GLAATTASLTRLMGKGEAQARRELMELRGDAVEVDV"
     gene            2580..>3065
                     /locus_tag="VFF72_07465"
     CDS             2580..>3065
                     /locus_tag="VFF72_07465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZV93040.1"
                     /translation="MRLRPTMLSDLEFVGSVEADPDNRPFITPWERVQHEGAIRFPDF
                     RHFIVEAGEAWPSAGFVILQGCRNPHGAVELKRLVLQRKGVGIGRTCVRLLAAMAFRD
                     LGAHRFWLDVKERNARALGLYRSEGFVEEGRLRDSVRDEAGWQALIVMSMLRAEHEAM
                     LE"
BASE COUNT          500 a         1012 c         1066 g          487 t
ORIGIN      
        1 tggctcagtc ggtcggcggt gtagttggaa gcgccgatgg cccgcacctt gccttcggcg
       61 atcagctcgc cgaaggccgc gagcgactct tcgagcggcg tcgagcggtc gtcttcgtgc
      121 gcctggtaga ggtcgatgac atcggtctga agacgccgca gcgaagcctc gacggcgtcg
      181 cggatgtagg cccgcgacag acccgatcga ccttcgccca tcggcttgcc gaccttggtg
      241 gcgaggacga cgcggttgcg cttgccactc ttcttcaacc acttgccgat gatcgtctcc
      301 gactcgccgc cggcatggcc gtggacccat ttcgagtaga cgtcggcggt gtcgacgaag
      361 ttcatgccgg cgtcgaccca ggcgtcgagc aaccggaacg acgtcgcctc gtcgacggtc
      421 cagccgaaga cattgccgcc gagacagatc ggcgaaacgt gcaggccgga gcggccgaga
      481 gggcgcagtg cgggcgtgtc catatcgagg cattgttgcg caggccggtt cgtcgcgtcg
      541 gacgcttcct agaatgccgt catggccagc aagcaatcca cttacggcga agcgtcgatc
      601 cgtgtcctga agggtctcga gccggtcaag cagcggccgg ggatgtacac ccgcaccgaa
      661 agtccgttgc acgtgatcca ggaggtgatc gacaacgccg ccgacgaggc gctcggcggc
      721 cacgcgacga ccatcgacgt tgtcctgcat ggcgatggct cgatcagcgt cgacgacgac
      781 ggtcgcggca tcccgttcgg cgtgcatccg gatgaaggcg tcagcgtcgt cgagatcgtc
      841 ttcactcgcc tgcacgcggg cggcaagttc gacaagggcg gtggcgctgc ctacagcttt
      901 tcgggcggct tgcatggcgt cggtgtcagc gtcaccaacg cgctggccag gcgcctcgag
      961 gtgaaggtct ggcgcggtgg ccaggtcgcg tcgatcgcct tcgccggcgg cgacgtggcg
     1021 gtgccgctcg aggtgcgaaa ggcgacagcg agcgaccgca agagcggcac gtcgatccgg
     1081 gtctggcccg atccgcgcta tttcgagaat gtcgagctgc cgcgcccgga gctgacgcac
     1141 ctgctgcgca gcaaggcggt gctgatgccc ggactcgccg tctccctggc gatcgagaag
     1201 gccggacgcg ccgatgacga gcgccagcag tggctctaca agagcggcct tgccgactat
     1261 ctgatgcagg cgctgccggc cgatcccgtg attcctctct tcgaaggcgc gcaccatgcc
     1321 gatggcggcg agagcgagga tgtcgccgaa ggcgagggcg cggcctggtg cgtcgccttc
     1381 accgaagaag gcgcgtcggt gcgcgagagc tacgtcaacc tcatcccgac ggtcgccggc
     1441 ggcacccatg agtcgggcct gaaggaaggt ctcttcgtgg cggtgaaggg attcatcgac
     1501 atgcacggcc tgctgcccaa gggcgtcaag ctgatggccg acgacgtgtt ctcgcgggcc
     1561 agcttcgtcc tctcggcgaa ggtgctcgac ccgcaattcc agggccagat caaggagcgg
     1621 ctgaacagcc gcgacgcgct tcgcctcgtc tcgaactgcg tccggccggc gctcgagctc
     1681 tggctcaacc agcacgtcga cctcggcaag aagctcgccg agatcgtcat ccgccaggcg
     1741 cagacccgcc agcgcgcgag ccagaaggtc gagaagaaga agggctcggg cgtcgccgtc
     1801 cttcccggca agctgaccga ctgcgagagc cgcgatccgg cgcgaagcga gatcttcctc
     1861 gtcgagggcg attcggccgg cggcagcgcc aagatggggc gcgacaagga gacgcaggcg
     1921 atcctgccgc tccgtggcaa ggtcctcaac gcctgggaag tcgagcgcga ccgcctgttc
     1981 gccaacaacg agatccacga catcgcggtg gcgctcggcg tcgatccgca cggcgccggc
     2041 gacgaccccg acctttcggg gctgcgctac ggcaaggtct gcatcctgtc cgatgccgac
     2101 gtcgacggct cgcacatcca ggtcctgttg ctgacgcttt tctttcgcca cttcccgaag
     2161 ctcatcgagc gcggccatgt ccacgtcgcc aagccgccgc tctttcgcgt cgacgcgccg
     2221 gcgcgcggca agaagccggc ggccaagatc tatgcgctcg acgaccgcga gctgcaggcc
     2281 gtcgtcgacc ggctgaagaa ggaaggcgcg cgcgacggct cgtggaccat cagccgcttc
     2341 aagggtctgg gggagatgaa tgccgagcag ctctgggaca cgacgctcaa ccccgagacg
     2401 cgccgactgc tgcaggtgag cttcggcgcc gccggcttgg ccgcgacgac cgcgtcgctg
     2461 acccgcctga tgggcaaggg cgaggcccag gcccgccgcg agctgatgga gctgcgcggc
     2521 gacgcggtcg aggtcgacgt gtgattgccg cggcgccgcc gcagggcccg gctctcccgg
     2581 tgcggctgag gcccaccatg ctgagcgatc tcgaattcgt cggctcggtc gaggccgatc
     2641 ccgacaatcg cccgttcatc acgccgtggg aacgtgtcca gcacgaaggg gcgatccgat
     2701 tcccggactt ccgtcacttc atcgtcgagg ccggcgaggc gtggccatcg gccggtttcg
     2761 tcatcctgca ggggtgccgc aatccgcacg gcgcggtcga gctcaagcgg ctcgtgctgc
     2821 agcgcaaggg cgtgggcatc ggccggacgt gcgttcgcct gctcgcggcg atggcctttc
     2881 gcgatctcgg cgcgcatcgc ttctggctcg acgtcaagga acgcaatgcg cgagccctgg
     2941 gcctgtatcg cagcgagggt ttcgtcgagg agggacgcct tcgcgacagc gtccgcgacg
     3001 aggccggctg gcaggcgctc atcgtgatgt cgatgctgcg cgccgagcat gaagcgatgc
     3061 tcgaa
//
LOCUS       DASRWS010000400         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_160206, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000400 DASRWS010000000
VERSION     DASRWS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160206"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFF72_07470"
     CDS             complement(<1..329)
                     /locus_tag="VFF72_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93041.1"
                     /translation="MTFLAALTTGAVMLIVSAASDWQADVGREVTIQVRPAPGRDLEA
                     DVRKAADIARATPGVADVRAFTKQESAQLVEPWLGGGLSLDDLPIPRLIVVKLASGML
                     PDFGALRR"
     gene            complement(466..1125)
                     /locus_tag="VFF72_07475"
     CDS             complement(466..1125)
                     /locus_tag="VFF72_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93042.1"
                     /translation="MIRFENVGLRYGLGPEVLRDVTFGIEGRSFQFLTGPSGAGKTSL
                     LRMMFLSLRPTRGLISMFDHDAATLSKDGRATLRRRIGIVFQDFRLLDHLTTYENVSL
                     PLRVLGKEEVQYRPEVVELLQWVGLGECMGALPPVLSGGEKQRTAIARAVIARPQLLL
                     ADEPTGNVDPNLGQRLLRLFIELNKSGTAVVIATHDIALMDEYDARRLVLHEGRLHVY
                     D"
     gene            1305..2309
                     /locus_tag="VFF72_07480"
     CDS             1305..2309
                     /locus_tag="VFF72_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HZV93043.1"
                     /translation="MLIVCPSCATSYDVELASLGPQGRQVRCSRCHMTWHAAPARADQ
                     LLAAAQAIAPALATELAVDGDGAEPPGEPAVEAPSEEASEVRRRIAAVDEPPDEEASE
                     SDAIAAPGDELGDGAASGDAGLSEESLEAAEVESPPIAPVDLDEGRPPIDIDAADFAE
                     RSIGPAMDVESVAARRFRLDLGSRARWPLSHLQTLILALVIVDAIVVGWRNDFVRLLP
                     HTAAFYALTGLSVNLRGLDFADVATTAEQHEGVPILVVQGSVLNTARKAVEVPRIKFI
                     ARNAARQEIYSWTVAPSRNVLSPGEAVAFRSRLASPPPDAQDVLVRFVNRRDVFAGTR
                     "
     gene            2318..2695
                     /locus_tag="VFF72_07485"
     CDS             2318..2695
                     /locus_tag="VFF72_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV93044.1"
                     /translation="MARILIAEDEEALRALCARALAAEGHEVNSACDGGEALDLLARE
                     QGRFDLLLTDIRMPIMDGIALALAAARDFPELTILLMTGYADQRERAQGLDAIIHDVI
                     AKPFTVAALRDTVSEALTVRARS"
BASE COUNT          465 a          963 c          931 g          438 t
ORIGIN      
        1 cgccgtaatg cgccgaagtc gggcagcatg ccggaggcga gcttgaccac gatgagacgc
       61 gggatcggga ggtcatcgag cgacagcccg ccgcccagcc agggctcgac caattgcgcc
      121 gattcctgct tcgtgaaagc gcgaacatcg gcgacgcccg gcgtcgcccg tgcaatatcc
      181 gccgccttgc gcacgtccgc ttcaaggtca cgccccggcg cgggacgaac ctggatcgtc
      241 acctcgcggc cgacatccgc ttgccagtcg cttgccgcgc tgacgatcag catcaccgcc
      301 ccggtcgtca gcgccgcgag aaacgtcatg atggcgacga cagcggcgag cgagcggccg
      361 gcgatcgagg tattgggaac gatcggtgtc gccgccggcg gcgccagcgc gagcggggtc
      421 gccgcttcga aatcgtccca tggctccgcc atggtctcga cgggcttagt catagacatg
      481 caggcggcct tcgtgcagca ccagccggcg cgcgtcgtat tcgtccatca aggcgatgtc
      541 gtgagtggcg atgacgactg ccgtccccga cttgttgagt tcgatgaaca gtcgcagcag
      601 gcgctgcccg agattgggat cgacgttgcc ggtcggctcg tcggcgagca gcagctgcgg
      661 gcgcgctatg acggcgcgcg caattgcggt gcgctgcttc tcgccgccgg aaagaaccgg
      721 cggcagcgcg cccatgcact cgccgagccc cacccattgc agcaattcga cgacctccgg
      781 ccggtattgc acctcctcct tgccgaggac gcgcagcggc agcgagacgt tctcgtaggt
      841 ggtcagatgg tcgagaagcc ggaagtcctg gaagacgatg ccgatccggc gccgcaaggt
      901 cgcccgcccg tccttgctga gcgtcgcggc atcgtgatcg aacatgctaa tcaggccgcg
      961 cgtcggccgc agcgatagaa acatcatgcg caggagcgag gtcttgcccg ccccggaggg
     1021 gccggtcagg aattgaaacg aacgcccctc gatgccgaag gtgacgtcgc gcaggacctc
     1081 cggaccgagc ccgtagcgca agccgacatt ctcgaaccgg atcacgccgt cctccgaagg
     1141 ctcctgccgc gagggcaaac cggcggcagg agccgtcccc tgattcgagc ccccagcaaa
     1201 cttaccacga cgccgcaatt actttcgcgg gcggcttggt ttccaatccg ttaacggatc
     1261 gcctgatagg gtcggcggga aggtgccgcg ctgctgattc gccgatgctg atcgtctgcc
     1321 cgtcctgcgc tacctcctat gacgtggagc tggcaagcct ggggccacag ggacgccagg
     1381 ttcgctgctc gcgttgccac atgacctggc acgcggcgcc ggcccgcgcc gaccagctcc
     1441 tcgccgcggc gcaggcgatc gcgccggcgc tcgccaccga actcgcggtg gacggggacg
     1501 gcgcggagcc gcccggcgag cccgccgtcg aggctcctag tgaagaggcg agcgaagtca
     1561 ggcgtcgcat cgccgcggtg gatgaacctc ctgacgagga ggcttccgaa agcgacgcca
     1621 tcgctgcgcc cggcgacgaa cttggggacg gagcggcaag cggtgacgcc ggtttgtccg
     1681 aagagtccct cgaagcggcg gaagtcgaat cgccgcccat cgcgccggtc gacctcgatg
     1741 agggccggcc gccgatcgac atcgatgccg ccgatttcgc tgaacgcagc atcgggccgg
     1801 ccatggatgt cgagagcgtc gccgcccgcc gcttccggct ggacctggga agccgggcac
     1861 gctggccgtt gtcccacctg cagaccctta tcctggcact ggtgatcgtc gacgccattg
     1921 tcgtcggttg gcgcaacgac ttcgtccgcc tgctcccgca taccgccgcg ttctacgccc
     1981 tgaccggcct gtcggtgaac ctgcgcggcc tcgatttcgc cgacgtggcc acgactgccg
     2041 aacagcacga gggcgtgccg atcctcgtcg tccagggcag cgtcctgaat acggcgcgca
     2101 aggccgtcga agtgccccgg atcaaattca tcgcccgcaa cgcggctcgg caggaaatct
     2161 attcctggac ggtcgcaccc tcgcgcaacg tgctgtcgcc aggcgaagcc gtcgcattcc
     2221 gcagccggct cgcttcgccg ccgccagacg cccaggacgt gctggtgcgc ttcgtcaatc
     2281 ggcgcgacgt gttcgccgga acgcggtgag gtcgcccatg gcgcgcattc tcatagctga
     2341 agacgaggaa gcgttgcgag cgctgtgcgc gcgggcgctc gcggccgagg gccatgaggt
     2401 caactctgct tgcgacgggg gcgaagcgct cgatctgctg gcgcgcgagc agggccgctt
     2461 cgatcttctt ctcaccgata tccgtatgcc gatcatggac ggcatcgcac tggcgctggc
     2521 ggcagcacgc gattttcccg agctcacgat cctgctgatg accggctatg ccgatcagcg
     2581 cgagcgcgcc caaggcctcg acgcgatcat ccacgatgtc atcgccaagc cgtttacggt
     2641 cgccgcgcta cgcgacaccg tcagcgaagc gttgaccgtg cgggcgcgct cgtaaggcgc
     2701 gcctgattgg tagccgcagc agcagcgcac gacgcttgcg tcacccatcg tcggacagcg
     2761 cataaggccg cggcaacggc tcgaccggca ggtccgg
//
LOCUS       DASRWS010000401         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3605338, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000401 DASRWS010000000
VERSION     DASRWS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3605338"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFF72_07490"
     CDS             complement(<1..698)
                     /locus_tag="VFF72_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZV93045.1"
                     /translation="MSAALLGAGVAVVGTAGAFDLPHWMKGDTQVASAQTQPADAHVV
                     PAAPLPIAPGQVPNYRAIVKQAAPAVVGITVEGTHKMSASEGGLPEGMENDPFFKFFR
                     GMPGFSGGNGQGSLSQPFHGLGSGFIIRSDGLILTNAHVVREAKEVTVKLSDRREYPA
                     KVLGTDPTTDIAVLKIDAKNLPVVRLGDARSLEVGDPVLAIGAPYGLEETATAGIVSA
                     KGRSLPGDAVVPFI"
     gene            complement(815..2122)
                     /locus_tag="VFF72_07495"
     CDS             complement(815..2122)
                     /locus_tag="VFF72_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93046.1"
                     /translation="MTSIRLRLLVSLLALLALAAAVMGFVTYRNVLAETESLFDYQLQ
                     QMALSLRDQGEIGAAQADSFTDARLDFVVQIWTVDGRSIYQSRPHPALPARALLGLAT
                     LNVQGHAWRTYSVATGDRVIQIAQPVDIRERLAARSAWRSILPLLLMAPLAALGIWWI
                     AAFQLRPLARLASEVRERDEQSLAPLATTGLPDELAPLAGALNTLLQRLGRSIDTQRA
                     FVADAAHELRSPLTALKLQLELLRRAPSEPDRVAARDAIGAGIDRATRLVEQLLALAR
                     SEPGAAPMAREPVDLAEIARVAVAETLPFATSRGIDFELVAAHPVIIVGDAVSLGLLV
                     RNLADNAARYSPPGSRVEVRVDTEDRGALLRVDDAGPGIPAADHDRVFDRFYRRADSA
                     APGSGLGLAIVRSVATMHGAALRLDRSPAGGLRVEVRFPGKPS"
     gene            complement(2119..2799)
                     /locus_tag="VFF72_07500"
     CDS             complement(2119..2799)
                     /locus_tag="VFF72_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV93047.1"
                     /translation="MRLLLVEDDRMIGESLQRALRLEGYAVDWVRDAAAAHGTLASER
                     FDLVLLDLGLPAGGPAGSGQADGLDVLRALRARHDATPVIVLTARDARGDRVAGLDAG
                     ADDYLVKPFELDELNARIRAVVRRHAGRSEPLLGVRGVTLDPASRRVTRDGVAVALSA
                     REFAVLEALLVRPGAVLSRTQLEDRLYGWGEEIESNAISVYVHQLRKKLGADFIRNAR
                     GVGYFIEP"
     gene            2871..4211
                     /locus_tag="VFF72_07505"
     CDS             2871..4211
                     /locus_tag="VFF72_07505"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZV93048.1"
                     /translation="MTARPRVLVIGCGFGGIEAVRALAKAEVDITLIDRTNHHLFQPL
                     LYQVATAGLSAPAVSAPIRHVLRHQMKRGHLTILQATAEHVDVAGRSVRVDGGQCLPW
                     DHLIVAAGATDSYFGHDEWTRFAPGLKTLADALDIRARIIGAYEHAERLDAGAERDAW
                     MTFAVIGGGPTGVEMAGTMTEIAQHTLRHEFRHIEPSRARVVLLEGADRILGAFVPAQ
                     SQKARQQLERLGVDVRMGAKVTGVDADGVDYAVAKGDAPGSQRLSCKTVIWAAGVAGS
                     SLGADLARGTGAALDRHGRIVVGPDLSLAGHPEIAVIGDLAAALSHGKGEPKPVPGVS
                     PAAKQMGRAAAANLSRRLRGEATQPFHYKDYGSLATIGRKAAIVELTVPFFGALRFSG
                     LAAWLFWLFAHIWFLIGFRNRIIVMVDWAWAYFTYERGARVVAGKVDEPSSSPP"
     gene            4279..>4651
                     /locus_tag="VFF72_07510"
     CDS             4279..>4651
                     /locus_tag="VFF72_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZV93049.1"
                     /translation="MDTRTSPARLRIERIRDVLRSEGCAAALIPSSDPHLSEYLPERW
                     QGREWASGFTGSMATLAVTLDGAALFADSRYWVQAERQLEGSGIELVRIPTPPGAQHI
                     EWLCEHVERGATVAVDGNVLGL"
BASE COUNT          704 a         1645 c         1660 g          642 t
ORIGIN      
        1 tggatgaacg ggacgaccgc gtcgccgggc agcgaacgcc ccttggcact gacgatgccc
       61 gcggtcgccg tctcctcgag gccgtaaggc gcgccgatcg ccagcaccgg gtcgccgact
      121 tcgagcgacc gggcgtcgcc gaggcggacc accggcaggt tcttggcgtc gatcttcagc
      181 acggcgatgt ccgtcgtcgg atcggtgccg agaaccttgg ccgggtattc gcgacgatcg
      241 gacagcttga cggtgacttc cttggcctcg cggacgacgt gcgcgttggt gaggatcagg
      301 ccgtcggagc ggatgatgaa gcccgagccg aggccgtgga atggctggct caacgaacct
      361 tgcccgttgc ctccggaaaa gcccggcatg ccgcggaaga acttgaagaa cggatcgttc
      421 tccatgccct cgggcaggcc gccttcgctg gccgacatct tgtgcgtgcc ctcgaccgtg
      481 atgccgacga cggcgggtgc cgcctgcttg acgatggccc ggtagttcgg cacctgaccg
      541 ggagcgatgg gcagaggcgc cgccggcacg acgtgcgcat cggcaggttg cgtctgggcc
      601 gaggcgacct gcgtgtcgcc tttcatccag tgaggaagat cgaacgcgcc cgcggtaccg
      661 accacggcca cgccggcccc aagcagggca gcgctcaatg ggttgagttt catagcgttt
      721 ctccgaaatg caatggcggg gcagggattg ccgcgaacgc agcaaagata cggccggcgg
      781 attagccggg cgttaggcga cgattaagcg acggttaaga cggcttgccg gggaaccgca
      841 cctcgacgcg cagcccaccg gccggcgatc gatccaggcg cagcgcggcg ccgtgcatcg
      901 tcgccacgct gcgcacgatc gccaggccca ggccgctgcc gggcgcggca ctgtcggcgc
      961 gccggtagaa ccggtcgaac acgcggtcat ggtcggccgc cggaatgccc ggcccggcgt
     1021 cgtcgacgcg cagcagcgcg ccgcggtctt cggtgtcgac ccggacctcg acgcgcgagc
     1081 ccggcggcga gtagcgcgcg gcgttgtcag cgaggtttcg caccagcaag ccgagcgaca
     1141 cggcgtcgcc gacgatgatc acgggatgcg ccgcgaccag ctcgaaatcg atgccccgcg
     1201 acgtcgcgaa cggcagcgtc tcggccaccg cgacgcgcgc gatctcggcc aggtcgaccg
     1261 gctcgcgcgc catcggcgcc gcgcccggct cgctgcgcgc cagggccagc aactgctcga
     1321 ccaggcgcgt cgcccggtcg atgccggcgc cgatcgcgtc tcgcgcggcg acgcggtcgg
     1381 gctcggaggg cgcccggcgc agcaactcga gctgcagctt cagcgccgtc aatggcgaac
     1441 gcagctcatg cgccgcgtcg gcgacgaagg cgcgctgcgt gtcgatcgag cggcccagcc
     1501 gctgcaacag cgtgttgagc gcaccggcca gcggcgccag ctcgtcagga aggccggtcg
     1561 tggcgagcgg cgcgagcgac tgctcgtcgc gttctcgcac ctcgctggcg agacgggcca
     1621 gcggccggag ctggaaggcg gcgatccacc agatgccgag cgccgcgagc ggcgccatca
     1681 aaagcagcgg caggatgctg cgccaggcgc tgcgcgcggc cagccgctcg cggatatcga
     1741 ccggctgcgc gatctggatg acccggtcgc ccgtggcgac gctgtacgtc cgccacgcgt
     1801 ggccctgcac gttgagcgtc gccagtccga gcagggctcg cgccggcagc gccggatgcg
     1861 gccgcgactg gtagatgctg cggccatcga ccgtccagat ctggacgacg aagtcgagcc
     1921 gggcgtcggt gaagctgtcg gcctgcgccg cgccgatctc gccctggtcg cgcagcgaca
     1981 gcgccatctg ctgcagctga tagtcgaaca gcgactcggt ctcggccagc acgttgcggt
     2041 aggtgacgaa gcccatcacc gcggcggcca gcgcgagcag cgcgagcagc gacacgagaa
     2101 ggcgcagccg gatcgaggtc atggctcgat gaagtagccg acaccgcgcg cgttgcggat
     2161 gaaatcggct cccagcttct tgcgcagttg atgcacgtag accgagatgg cgttgctctc
     2221 gatctcctcg ccccagccgt agagtcggtc ctcgagctgg gttcgcgaca gcaccgcgcc
     2281 gggccggacc agcaacgcct cgaggaccgc gaactcgcgc gccgacaggg cgacggcgac
     2341 gccgtcgcgg gtgacgcgcc ggctcgccgg atcgagcgtc acgccgcgca cgccgagcag
     2401 cggctcggag cgtccggcgt ggcgccggac gacggcgcgg atgcgcgcat tcaactcgtc
     2461 gagctcgaac ggcttgacga gatagtcgtc ggcaccggcg tcgaggccgg cgacccgatc
     2521 gccgcgcgcg tcgcgcgcgg tcaggacgat gaccggcgtc gcgtcgtgtc gggcacgcag
     2581 cgcgcgcagc acgtcgaggc cgtcggcctg gcccgatccg gccgggccac ccgccggcag
     2641 gccgaggtcg agcaggacga gatcgaagcg ctcgctcgcc agcgtgccgt gcgcggcggc
     2701 cgcatcgcga acccagtcga ccgcgtagcc ctcgaggcgc agcgcgcgct gcaggctctc
     2761 gccgatcatg cggtcgtctt ccaccagcag caggcgcata gtcgatcgag catagcgctg
     2821 cgggcctgcc ggcggagaat gcgggccatg gtcatcttcg caacaggcac atgacggccc
     2881 ggccacgcgt cctcgtcatt ggctgcggct tcggcggcat cgaggcggtg cgcgcattgg
     2941 cgaaagccga ggtcgacatc acgctcatcg accgcaccaa tcaccacctg ttccagccgc
     3001 tgctctacca ggtggcgacc gccggcctca gcgccccggc ggtcagcgcc ccgatccggc
     3061 acgtgctgcg ccaccagatg aagcgcggcc atctgacgat cctgcaggcg accgccgaac
     3121 acgtcgacgt cgcgggccga agcgtccgcg tcgacggcgg ccagtgcctg ccctgggacc
     3181 atctcatcgt cgctgccggc gccaccgaca gctacttcgg ccacgacgag tggacccgct
     3241 ttgcgccggg gctgaagacg cttgccgacg cgctcgacat ccgggcccgc atcatcggcg
     3301 cctacgagca cgccgagcgg ctcgacgccg gcgccgagcg cgacgcgtgg atgacgttcg
     3361 ccgtcatcgg cggcggtccg accggcgtcg agatggccgg cacgatgacc gagatcgccc
     3421 agcacacgct gcgccacgaa ttccggcaca tcgaaccgtc tcgcgcccgg gtcgtgctgc
     3481 tcgaaggtgc cgatcgcatc ctcggcgcgt tcgtgccggc gcagtcgcag aaggcccgcc
     3541 agcaactcga gcgactcggc gtcgacgtgc gcatgggcgc gaaagtgacc ggggtcgatg
     3601 ccgacggggt cgactatgcg gtggcgaagg gcgatgcgcc agggtcgcag cggttgtcgt
     3661 gcaagaccgt gatctgggct gccggcgtcg ccggatcgtc gctcggcgcc gatctcgcgc
     3721 gcggcacggg tgccgccctg gaccgtcacg gccgcatcgt cgtcgggccc gacctgagcc
     3781 tcgccggcca ccccgagatc gccgtcatcg gcgatctcgc cgccgcgctc agtcatggca
     3841 agggcgagcc gaaaccggtg cccggcgtca gcccggccgc caagcagatg ggtcgcgccg
     3901 ccgcggcgaa cctgagccga cggctgcgtg gcgaggcgac ccagccgttt cactacaagg
     3961 actacggcag cctcgccacg atcggccgca aggcggcgat cgtcgagctg accgtgccgt
     4021 tcttcggcgc gctgcgcttc agcggcctgg ccgcatggct cttctggctc ttcgcgcaca
     4081 tctggttcct gatcggcttt cgcaaccgga tcatcgtcat ggtcgactgg gcctgggcgt
     4141 acttcacgta cgagcgtggc gcgcgggtcg tggcgggcaa ggtcgatgag ccctcgtcgt
     4201 cgccgccctg agagacggcg cccgacgcca ccgagcttca ttaaactggt accgcccgtt
     4261 cgatcaccgg atttcgccat ggacacccgc acctcccccg cccggctgcg catcgagcgc
     4321 atccgcgacg tcctcaggtc cgaaggctgc gccgcggcgc tgatcccgtc gagcgatccg
     4381 cacctgtcgg agtacctgcc ggagcgctgg caggggcggg agtgggcgtc gggcttcacc
     4441 gggtcgatgg ccacgttggc ggtcacgctc gacggcgcgg cgctcttcgc cgacagccgc
     4501 tactgggtgc aggccgagcg ccagctcgag ggcagcggca tcgagctggt ccggatcccg
     4561 acgccgcccg gcgcgcagca catcgaatgg ctttgcgagc acgtcgaacg cggcgcgacg
     4621 gtcgcggtcg acggcaacgt gctcgggctg g
//
LOCUS       DASRWS010000402         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1306657, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000402 DASRWS010000000
VERSION     DASRWS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306657"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFF72_07515"
     CDS             complement(<1..942)
                     /locus_tag="VFF72_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZV93050.1"
                     /translation="MNLPMTGHRPSPLLEGLNPEQLAAVTLPPEPALILAGAGSGKTR
                     VLTTRIAWLIQTGQLSPGGVLAVTFTNKAAKEMLTRLDAMLAIPVRGMWVGTFHGLCN
                     RFLRAHWKMAALPQGFQILDTADQLSAVKRVMKAMNLDEERFVPKQITWQIAGAKDDG
                     LRPRDVEQRDEQSRVMVEVYEAYEAQCQREGVVDFAELMLRSYELMRDNAALREHYQR
                     RFRHILVDEFQDTNKLQYAWLKMFAGPPELGSALFCVGDDDQSIYAFRGAQVGNMAAF
                     ERDFRVEQVIKLERNYRSFGNILDAANELIAHNSRRLG"
     gene            1033..2010
                     /locus_tag="VFF72_07520"
     CDS             1033..2010
                     /locus_tag="VFF72_07520"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HZV93051.1"
                     /translation="MEIFDYDNVLLLPRKCRVESRSQCDASQAFGPRRFRLPVVPANM
                     KTVIDEPIAEWLAANDYFYVMHRFDVDNVAFARKMRDRKLFVSISSGVKTADYSAIDR
                     LAADGVGADYVTIDIAHGHADTVHRMIEHIRKKLPKAFIIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITRLKTGFGTGGWQLSALKWCARVATKPIVADGGIRDHGDIAKSV
                     RFGASMVMIGSLFAGHEESPGKTVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPV
                     KGHLADTLREMTEDVQSSISYAGGTTLADIRRVNYVILGGDNAGEHLLM"
     gene            2052..3353
                     /locus_tag="VFF72_07525"
     CDS             2052..3353
                     /locus_tag="VFF72_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HZV93052.1"
                     /translation="MNASLKAAGAALLVAAAVAACGGGSLSVTQGHVRLVNATHGIGA
                     LDLYQGSSLISSNIAAGTAGAYADLDPGSYTFNLNLAGTSATAATIAGTVQKKDDYTL
                     VAYTTGGTLTGTYIAENEGSPSSKSAKLRLFNADATDVPSVDAYLLTTDCSDLASSPA
                     APIITAVTGLQPSYTQVNAASGGTAYHLCVTTAGDKSDLRLDIPSLTLSDGQIVTVIL
                     TASSGGVLLDGLVLNQGGTLVAATSASTRIRLAVGTTDGSLVTASVNGVSLGTQLPAP
                     AVGSYVLVPSGPITLVATIGGVAVPDPGLTATPGADMTLLIAGTASTPPVLITDNNSV
                     STSTANPVKLRLVNGMNTTGPATLTDNFINVGVPAAFGASSGYGQVQASTALAQLQAT
                     IGAMQLCQSANVTLTAGDVYTVFLLGNVPSSPTACTITEDR"
     gene            3393..>4521
                     /locus_tag="VFF72_07530"
     CDS             3393..>4521
                     /locus_tag="VFF72_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZV93053.1"
                     /translation="MNDLAKSFDPAAIEAHWGPLWEESGIYEPTLDPSRPSFCIQLPP
                     PNVTGTLHMGHAFQHTIMDSLVRYHRMKGDNTLWVPGTDHAGIATQIVVERQLQETGL
                     SRHDVGRKNFLAQVWDWKDTSGSTITRQMRRMGDSVAWKYEYFTMDSRLSEVVTETFV
                     KLHEEGLVYRGKRLVNWDPVLLSAVSDLEVENEEREGTMWHILYPFSDGPQPGADGRP
                     MRGMHIATTRPETMLADGALAVHPDDERYRHLVGKMVDLPLCDRQIPVIADAYVDKDF
                     GSGCVKITGAHDFNDYQVAQRHGLPLIVIFTLSATINDNGPAAYRGLDRYDARKAVLK
                     DLEAQGFLEKAVPHRHAVPVCVRTGQVVEPMLTDQWFMAMTR"
BASE COUNT          777 a         1585 c         1465 g          694 t
ORIGIN      
        1 gccgagccgc cgcgagttgt gcgcgatcag ctcgttggcg gcgtcgagga tattgccgaa
       61 ggagcggtag ttgcgctcga gcttgatgac ctgctcgacg cggaaatcgc gctcgaacgc
      121 cgccatgttg ccgacctggg cgccgcgaaa ggcgtagatg ctctggtcgt cgtcgccgac
      181 gcaaaagagc gccgagccga gctcgggcgg cccggcgaac atcttcagcc acgcgtactg
      241 gagcttgttc gtgtcctgga actcatcgac caggatgtgc ctgaagcggc gctggtagtg
      301 ctcgcgcagg gcggcgttgt cgcgcatcag ctcgtagctg cgcagcatca gctcggcgaa
      361 atcgacgacg ccctcgcgtt ggcactgcgc ctcgtacgcc tcgtacacct cgaccatgac
      421 gcggctttgc tcgtcgcgct gctcgacgtc gcgcgggcgc agcccgtcgt ccttggcgcc
      481 ggcgatctgc caggtgatct gcttggggac gaagcgctcc tcgtcgaggt tcatcgcctt
      541 catgacgcgc ttgacggccg agagctggtc ggccgtgtcg aggatctgga aaccctgcgg
      601 caacgcggcc atcttccagt gcgcgcgcag gaaccggttg cacaggccgt ggaaggtgcc
      661 gacccacatc ccccgcacgg gaatcgcgag catcgcgtcg agacgggtca gcatctcctt
      721 ggcggccttg ttggtgaagg tcaccgccag gacgccgccg ggcgaaagct ggccggtctg
      781 gatcagccag gcgatgcggg tcgtcaggac gcgcgtcttt ccggagccgg ctccggcgag
      841 gatcagcgcg ggttcgggcg gcagggtgac ggcggcgagc tgctcgggat tgaggccttc
      901 gagcagcggc gacgggcgat gtcccgtcat cggaagattc atcggcgcat tctaggtacc
      961 tcgccgcgaa cggcacctgc gtagaatgcg tcaacgggca ctcgtcgagg tgcccgttct
     1021 tgcgtctgca tcatggaaat cttcgactac gacaacgtcc tgctgctgcc gcgcaagtgc
     1081 cgcgtcgaat cgcgctcgca gtgcgacgca tcgcaggcgt tcgggccgcg ccggttcagg
     1141 ctgccggtcg tgccggcgaa catgaagacg gtgatcgacg agccgatcgc cgaatggctc
     1201 gccgccaacg actacttcta cgtgatgcac cgcttcgacg tcgacaacgt cgccttcgcg
     1261 cggaagatgc gcgacaggaa gctcttcgtc tcgatcagct ccggggtgaa gacggcggac
     1321 tactcggcga tcgaccgtct cgccgccgac ggcgtcggcg ccgactacgt gacgatcgac
     1381 atcgcccacg gccacgccga caccgtgcac cgcatgatcg agcacatcag gaagaagctc
     1441 ccgaaggcgt tcatcatcgc cggcaacgtc gccacgcccg aggccgtgat cgatctcgag
     1501 aactggggcg ccgacgcgac caaggtcggc gtcggcccgg gcaaggtctg catcacccgg
     1561 ctgaagacgg ggttcggcac cggcggctgg cagctttcgg cactgaagtg gtgtgcccgt
     1621 gtcgccacca agccgatcgt cgccgacggc ggcatccgcg atcacggcga catcgccaag
     1681 agcgtgcgct tcggcgcctc gatggtgatg atcggctcgc tcttcgcggg ccacgaggaa
     1741 tcgccgggaa agaccgtcga ggtcgacggc aagctcttca aggagtacta cggctcggcc
     1801 tcggacttca acaagggcga atacaagcac gtcgagggca agcgcatcct cgagccggtc
     1861 aaggggcatc tggccgacac gctgcgcgag atgaccgagg atgtgcaaag ctcgatctcg
     1921 tacgccggtg gcaccaccct ggccgacatc cgcagggtga actacgtcat cctcggcggc
     1981 gacaacgcgg gcgaacacct gctgatgtga cccgcgcccc gcgcgaaagg gccgggaacg
     2041 aaaggaatgt cgtgaatgca tcgttgaaag cggccggggc cgccctcctc gtcgcggccg
     2101 ccgtcgccgc ctgcggcggc ggcagcctgt ccgtcaccca ggggcacgtg cggctcgtca
     2161 acgccaccca cgggatcggc gcactcgacc tctaccaggg atcgagcctg atcagctcga
     2221 acatcgctgc cggcacggcg ggcgcctacg ccgatctcga cccgggttcc tacaccttca
     2281 acctgaacct cgcaggaacg agcgccaccg cggccacgat cgcgggcacg gtgcagaaga
     2341 aggacgacta caccctggtc gcctacacga ccggcggcac gttgaccggc acctacatcg
     2401 ccgagaacga aggatcgccg agctcgaaat cggccaagtt gcggctcttc aacgccgacg
     2461 cgaccgacgt gccgagcgtc gacgcgtacc tgctcaccac cgactgctcg gacctcgctt
     2521 cgtcgcccgc ggccccgatc atcacggcgg tcacgggcct gcagccttcc tacacccagg
     2581 tcaatgccgc cagcggcggc accgcctatc acctgtgcgt cacgacggcc ggcgacaaga
     2641 gcgacctgcg gctcgacatc ccttcgctga ccctgtcgga cgggcagatc gtcaccgtca
     2701 tcctgaccgc gagcagcggt ggcgtcctcc tcgacggcct ggtgctcaac cagggcggca
     2761 ccctggtggc ggcgacgagc gcctcgacgc ggatccgcct cgccgtgggc accacggacg
     2821 gctcactcgt caccgcgtcg gtcaacgggg tgtcgctcgg cacgcaactg ccggcgcccg
     2881 cggtcggcag ctacgtgctg gtgccgtcgg ggccgatcac gctggtcgcg acgatcggcg
     2941 gcgtcgccgt cccggatccg ggtctgacgg ccaccccggg cgccgacatg acgctgctga
     3001 tcgccggcac cgcttcgacg ccgcccgtcc tcatcaccga caacaattcc gtctcgacca
     3061 gcaccgccaa tccggtcaag ctgcgtctcg tcaacggcat gaacacgacg ggcccggcga
     3121 cgctgaccga caacttcatc aatgtcggcg tccccgccgc attcggcgcc tcgtcaggct
     3181 acggccaggt gcaggcgagc acggcgctgg cgcagctgca ggcgacgatc ggggcgatgc
     3241 agctctgcca gtcggccaac gtcacgctca ccgccggcga cgtctacacc gtcttcctgc
     3301 tcggcaacgt gccctcgtcg ccgaccgcct gcacgatcac cgaggatcgc tgagacgccc
     3361 tcgtcgcttc cctcgccgca ccgccagccc agatgaacga cctcgccaaa tccttcgacc
     3421 ccgccgccat cgaggcccac tggggcccgc tctgggaaga gagcggcatc tacgagccca
     3481 cgctcgaccc gtccaggccc tcgttctgca tccagctgcc gccacccaat gtcaccggca
     3541 cgctgcacat gggccacgcg ttccagcaca cgatcatgga ttcgctggtt cgctaccacc
     3601 gcatgaaggg agacaacacc ctctgggtgc ccggcaccga ccacgccggc atcgcgacgc
     3661 agatcgtcgt cgagcgccag ttgcaggaga ccggcctgag ccggcacgac gtcgggcgaa
     3721 agaacttcct cgcccaggtc tgggactgga aggatacgag cggctcgacg atcacgcggc
     3781 agatgcgtcg catgggcgac tcggtggcgt ggaagtacga gtacttcacg atggattcgc
     3841 ggctctcgga ggtcgtcacc gagaccttcg tcaagctgca cgaggaaggt ctcgtctacc
     3901 gcggcaagcg tctcgtcaac tgggacccgg tgctcctttc ggcggtctcc gacctcgagg
     3961 tcgagaacga ggagcgcgag ggcacgatgt ggcacatcct ctatccgttc agcgacgggc
     4021 cgcagccggg tgccgacggc aggccgatgc gcggcatgca catcgccacg acgcgcccgg
     4081 agaccatgct cgccgacggc gccctcgcgg tgcatcccga cgacgagcgc taccggcacc
     4141 tcgtcggcaa gatggtcgac ctgccgctgt gcgaccggca gatcccggtg atcgccgacg
     4201 cctacgtcga caaggatttc ggctcgggct gcgtcaagat caccggcgcc cacgacttca
     4261 acgactacca ggtcgcacag cgccacggcc tgccgctgat cgtcatcttc acgctttcgg
     4321 cgacgatcaa cgacaacggg cccgccgcct atcgcggcct cgaccgctac gacgcgagga
     4381 aggctgtgct gaaggacctc gaggcgcagg gcttcctcga gaaggcggtg ccgcatcgcc
     4441 atgccgtgcc ggtctgcgtg cgcaccggcc aggtcgtcga gccgatgctc accgaccagt
     4501 ggttcatggc gatgaccagg c
//
LOCUS       DASRWS010000403         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_741928, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000403 DASRWS010000000
VERSION     DASRWS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741928"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFF72_07535"
     CDS             complement(<1..588)
                     /locus_tag="VFF72_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV93054.1"
                     /translation="MTETSGPFTKTPGWLDWYKNPSKPVFEVPPGSVDAHCHVFGPGA
                     VFPYAPERKYTPCDASKAQLFALRDRLGFARNVVVQATCHGADNRAMVDALRHSHGMA
                     RGVATVRRSVTDAELSDMHAAGVRGVRFNFVKRLVDFTPKDELLEIASRIAPLGWHVV
                     IYFEAVDLPELIDFFTALPTTVVVDHMGRPDVSLPV"
     gene            complement(585..1613)
                     /locus_tag="VFF72_07540"
     CDS             complement(585..1613)
                     /locus_tag="VFF72_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HZV93055.1"
                     /translation="MSASSPRDWRIGLVGYGEVGRILAEDLRQRGSHVSAFDLKLASG
                     GGGGSGAAAEAGAAMREHAQSHGVTLAASHLELAASADLVISAVTASQAVPVAEACAP
                     GIRAGAFFLDFNSASPGAKIRAARVIEAAGGRYVEGAVMTSIPPYRIKVPLLLGGPNA
                     AALEPALDALGFAARVASEKLGVASATKMCRSVMIKGLEAMVIESFTTARAYGVEDAV
                     LASLKETFPGIDWEKQGAYFFQRVIEHGRRRSEEVREVAETVREAGLTPWSATGTAER
                     QAWVADLADEGVFGRRGASGFARSADWRTEADRLLARTKSPDRSSARASAAPPAEPKG
                     ASAKKDSA"
     gene            complement(1624..2316)
                     /gene="ligK"
                     /locus_tag="VFF72_07545"
     CDS             complement(1624..2316)
                     /gene="ligK"
                     /locus_tag="VFF72_07545"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HZV93056.1"
                     /translation="MALPNTLGVVKRSIARADRAAVDKLARFGVATVHEAMGRVGLMK
                     PYMRPIYPHAAMCGTAVTVLLQPGDNWMLHVVAEQLQPGDVVVGACTTDNDDGFFGDL
                     LATSFKARGARGLVIDAGVRDVAELEKMGFPVFSRAIHAKGTVKATLGSVNVPVVCAG
                     AYVNPGDVVVADADGVVVVPAAIAQQVADAAERREANEGEKRAKLAAGVLGLDMYDMR
                     GPLEKAGLRYID"
     gene            complement(2319..>2452)
                     /locus_tag="VFF72_07550"
     CDS             complement(2319..>2452)
                     /locus_tag="VFF72_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZV93057.1"
                     /translation="YDDTKRYIEASTIVVGDDRHQIYEGNARRVYPRLDAALKQKGH"
BASE COUNT          358 a          834 c          880 g          380 t
ORIGIN      
        1 gaccggcagg ctcacgtcgg gccgccccat gtggtcgacg acgacggtcg tcggcagcgc
       61 ggtgaagaag tcgatcagct cgggcaggtc gacggcctcg aagtagatca ccacgtgcca
      121 gccgagcggg gcgatgcgcg aggcgatctc gagcagctcg tccttcggcg tgaagtcgac
      181 gaggcgcttg acgaaattga atctaacgcc gcgcacgccg gcggcgtgca tgtcgctcaa
      241 ctcggcatcg gtgacgctgc gccgcaccgt ggcgacgccg cgcgccatgc catgcgagtg
      301 ccgcagcgcg tcgaccatcg cccggttgtc ggcgccgtgg caggtggcct ggacgacgac
      361 gttgcgcgcg aagccgagcc gatcgcgcag cgcgaagagc tgcgccttcg acgcgtcgca
      421 cggcgtgtac ttgcgctcgg gggcataggg gaagacggcg cccgggccga agacgtggca
      481 gtgcgcatcg accgatccgg gcggcacctc gaagacgggc ttcgacgggt tcttgtacca
      541 gtcgagccag ccgggcgtct tcgtgaaggg gccggaggtt tcggtcatgc ggagtccttc
      601 ttcgcgctcg cgcccttcgg ctccgcgggc ggcgcggccg aagcgcgggc gctcgatcga
      661 tcaggcgatt tcgtgcgggc aaggaggcga tcggcctcgg tgcgccagtc ggcactgcgc
      721 gcgaagccgc ttgcgccgcg ccggccgaac acgccttcgt cagcaaggtc ggcgacccag
      781 gcctgtcgtt cggccgtgcc ggtcgccgac cacggcgtga gcccggcctc gcgcacggtt
      841 tcggcgacct cgcgcacctc ttcgctgcgc cgccgtccat gctcgatcac gcgctggaag
      901 aaatacgcgc cctgcttttc ccagtcgatg ccgggaaacg tttccttcag cgaagcgagc
      961 accgcgtctt cgacgccgta ggcgcgcgcc gtcgtgaagc tctcgatgac catcgcctcg
     1021 aggcccttga tcatgacgct gcgacacatc ttggtggccg aggcgacgcc gagcttctcg
     1081 ctcgcgacgc gcgccgcgaa gccgagcgcg tcgagcgccg gttcgagcgc ggcggcgttc
     1141 gggccgccga gcagcagcgg caccttgatg cgatacggcg ggatcgacgt catcaccgcg
     1201 ccttcgacgt agcggccgcc ggccgcttcg atcacccggg cggcgcggat cttcgcaccg
     1261 ggcgatgccg agttgaagtc gaggaagaag gcgccggcgc ggatccccgg cgcacacgcc
     1321 tcggcgaccg gaacggcctg gctcgcggtg acggccgaaa tgacgaggtc ggcgctggcg
     1381 gcgagctcga gatggctggc ggcgagcgtc acgccgtgcg actgcgcgtg ttcgcgcatc
     1441 gcggcaccgg cttcggccgc ggcgccgctg ccaccgccac cgccactggc gagcttcaga
     1501 tcgaaggcgc tgacatggct gccgcgctgg cgcaggtctt cggcgaggat gcggccgacc
     1561 tcgccatagc cgacgagccc gatccgccag tcgcggggag aagaagcact catcgcggtc
     1621 ggtctagtcg atgtacctga ggccggcctt ctcgagcggg ccgcgcatgt cgtacatgtc
     1681 gaggccgagc acgccggcgg cgagcttggc gcgcttctcg ccttcgttcg cttcgcgcct
     1741 ttcggccgcg tcggcgacct gctgcgcaat cgccgccggc acgacgacga cgccatcggc
     1801 gtcggcgacg acgacgtcgc cgggattgac gtaggccccg gcgcagacga ccgggacgtt
     1861 gaccgagccg agcgtcgcct tgaccgtgcc cttggcgtgg atggcgcggc tgaacaccgg
     1921 aaatcccatc ttctcgagct cggccacatc gcgaacaccg gcgtcgatga cgaggcccct
     1981 ggcgccgcgc gccttgaacg aggtggcgag cagatcgccg aagaagccgt cgtcgttgtc
     2041 ggtcgtgcag gcgccgacga cgacgtcgcc gggctgcagt tgctcggcga cgacgtgcaa
     2101 catccagttg tcgcccggct gcagcaggac ggtgacggcg gtgccgcaca tcgccgcatg
     2161 cggatagatc ggccgcatgt acggcttcat caggccgacg cggcccatcg cctcgtgcac
     2221 ggtcgcgacg ccgaaacggg ccagcttgtc gacggcagcg cgatcggcgc gggcgatgct
     2281 gcgcttgacg acgcccaggg tgttcgggag ggccatggtc agtgtccttt ctgcttgagc
     2341 gccgcatcga gtcgtggata gacgcgtcgc gcgttgcctt cgtagatctg gtgccggtcg
     2401 tcgccgacga cgatcgtcga cgcctcgatg tagcgcttcg tgtcgtcgta gt
//
LOCUS       DASRWS010000404         8069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1610500, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000404 DASRWS010000000
VERSION     DASRWS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610500"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VFF72_07555"
     CDS             <1..124
                     /locus_tag="VFF72_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein"
                     /protein_id="HZV93058.1"
                     /translation="QLALSGADRGYVLETGRVVHSGQAKALIDDPAVRQAYLGL"
     gene            complement(235..1020)
                     /locus_tag="VFF72_07560"
     CDS             complement(235..1020)
                     /locus_tag="VFF72_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93059.1"
                     /translation="MAKWTAFPHSAETYTHDIAALKKKWPRLHAGDAEPLPKDESVLA
                     AWALFHAGQFQKATEAGLKAHAAGAGAGMTVANKSQAIYATYLEKSEKTKLALLHEVA
                     ERAEARTREDPKDANAWYCMAYALGRYSQGISIAKALAQGLGAKVKTALETTIRLSPR
                     HADAHIALGAFHAEIIDKVGSLLGRTQGASKEAGLASYKAALRLNPGSAIAMIEYANG
                     LVMLEGEKKMKEATKLYEDAAATTPLDAMERLDVEMAKAELEE"
     gene            complement(1031..1708)
                     /locus_tag="VFF72_07565"
     CDS             complement(1031..1708)
                     /locus_tag="VFF72_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV93060.1"
                     /translation="MATPSASRKKKPRRTAERILEAALELFNRFGEPNVSTTLISAEL
                     GISPGNLYYHYPAKEELVTRLFERYDAALVELLRAAEDVGHIEDAWLFFHLLFELIWR
                     YRFLYRDLNDLLANNRKLELHFPFVLQRTSRAVQAVLDGLAQTGGVAVERRDSEPVAT
                     AMVVVLSWWLSFEYVQNPRRALEAESAGPALLRGAYHVLCLLRPYLDATGREHLDALA
                     SRYLSSP"
     gene            1817..2740
                     /locus_tag="VFF72_07570"
     CDS             1817..2740
                     /locus_tag="VFF72_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93061.1"
                     /translation="MPAVSFQNVVKVYPSSGGGLRALDDVSFDVEPGEFFGLLGPNGA
                     GKTTLISILAGLARATSGRIAVLGHDVAGDYAAARRRLGIVPQELVFDPFFTVREALR
                     IQSGYFGVHGNDAWIDELLLNLGLEDKADANMRQLSGGMKRRVLVAQALVHRPPVIVL
                     DEPTAGVDVELRQTLWQFVARLNKEGHTVLLTTHYLEEAEALCGRIAMLKQGRIVALD
                     RTTALLAGTASTMLQFKLDRPLPPALAAHARVTGRIVQLKAHDAREVEAILAALHAAG
                     CEPEDLEIGRADLEDVFIEIMHGETPERVAA"
     gene            2737..3549
                     /locus_tag="VFF72_07575"
     CDS             2737..3549
                     /locus_tag="VFF72_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93062.1"
                     /translation="MTTTQVGAATVRVETEHGVLAGARTLLYKEVLRFWKVSFQTVAA
                     PVLTAVLYLLIFGHVLEGRVKVFGSVGYTSFLIPGLVMMSVLQNAFANSSSSLIQSKI
                     TGNLVFLLVTPLSHWAWFLAYVGASVVRGIAVGTGVMLVTIWFAPLDLANPLWVLVFT
                     LAGATLMGALGLVAGLWAEKFDQIAAFQNFLIMPMTFLSGVFYSVHSLPPTWMAVSHL
                     NPFFYMIDGFRRGFFGTSDVSPWISLVVVGAALVLICGLCLHLLKIGYKIRH"
     gene            4002..5723
                     /locus_tag="VFF72_07580"
     CDS             4002..5723
                     /locus_tag="VFF72_07580"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HZV93063.1"
                     /translation="MTTGMKKGLTAYGDAEFSLFLRKAFIKAMGLSDDALNRPIVGIA
                     NTFSDYNACHGNVPRLVEAVKRGVMLAGGLPMEFPTISLHESFAFPTSMFLRNLMAMD
                     TEEMLRAQPMDAVVLIGGCDKTLPAQMMAAASADVPAIVVPVGPMLVGHHKGETLGAC
                     TDCRRLWGEFRAGRIDAAEIEAINGRLAPTAGTCMVMGTASTMACIVETMGLGLAQSA
                     TIPATHADRLRSAEASGRCAVALAAGTGPRPSALMTRAAFRNALVVMQAIGGSTNALI
                     HLTAVAGRLGQRIDLDEVDRLGREVPVLVDLKPSGRHYMEHFHWAGGVPRLLHELAPW
                     LDLDAPTVEGATLRTRIDGIDTATPQDVIRPCANPIHAGGSMAVLRGNLAPRGAILKQ
                     SAASPALLQHEGRAVVFDSLEDLAARIDSADLDVAAGDVLVLKNAGPIGAPGMPEAGY
                     LPIPKKLAAQGVKDMVRLSDARMSGTAFGTIVLHMTPEAALGGPLAQVRSGDRIRLDT
                     AARRIDLLVDAAEIERRREEFVRVGSPGAAGRPDRGYAALFHRSVLQADEGCDFDFLV
                     PGFSRRD"
     gene            5976..6725
                     /locus_tag="VFF72_07585"
     CDS             5976..6725
                     /locus_tag="VFF72_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZV93064.1"
                     /translation="MRHLFSGLLASLALGAAALLSPVTASAVTVATIGGCYDCGVYDT
                     ASLIFDNTTGGTLTNAQMLLTGYQGQNNGQTATVNLGTLSAGSTQIFWGNLPGVSSST
                     TPNNLTAYDYDDEFINTSAIINDPTCGGGGCVDGGGQQWYAQVGNFSVTFTAVVTGGA
                     YDGQSVYSVFSPTSNATGGFVGWEGLDPNGYSESPQYDVHTGIVTGHLADIDLGLPPP
                     PVPEPETYALMLAGLGALGFLRRRGVKSPRG"
     gene            7015..7266
                     /locus_tag="VFF72_07590"
     CDS             7015..7266
                     /locus_tag="VFF72_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HZV93065.1"
                     /translation="MADPTPAEIERFITDGLACELVEVEGDGRHFHATIVSAEFEGAN
                     RVGRHQRVYRALGERMREQIHALSMQTLTPAEYRNRPTA"
     gene            7290..>8069
                     /locus_tag="VFF72_07595"
     CDS             7290..>8069
                     /locus_tag="VFF72_07595"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZV93066.1"
                     /translation="MDKLRIRGGRRLAGEVTISGAKNAALPELCAALLTAEPVLLSNV
                     PRLRDVATMLRLLRTMGVTAEESNDAEASVSLDARKIDRREAAYDLVKTMRASILVLG
                     PLLARFGEARVSLPGGCAIGTRPVDQHIKGLQALGAEISVEHGYIVARASRLKGARIT
                     TDMVTVTGTENLLMAATLAEGETVLENAAQEPEVADLAQMLIAMGARIEGHGTSRIRV
                     EGVRELHGTSHRIIADRIEAGTFLCAVAATGGDVLLRRARAD"
BASE COUNT         1183 a         2823 c         2719 g         1344 t
ORIGIN      
        1 ccagctcgcg ctctccggcg ccgatcgcgg ctatgtgctc gagaccggcc gcgtcgtgca
       61 cagcggccag gccaaggcgc tgatcgacga cccggccgtc cgccaggcgt atctcggcct
      121 gtaggaccgc tggccggcga ggctgtcccg gcccgtcggc gcggcgccat gtcggcgtct
      181 tccaggcgac ggtgcgaaga ggtcgcggct ccccgggcgc atcgacgacg ttcttcactc
      241 ttcgagctcg gccttcgcca tctcgacgtc gagccgctcc atcgcgtcga gcggcgtcgt
      301 cgccgcggcg tcctcgtaga gcttcgtcgc ttccttcatc ttcttctcgc cttcgagcat
      361 gaccagtccg ttggcgtact cgatcatggc gatcgccgag ccggggttga gcctgagggc
      421 ggccttgtag ctcgcgaggc ccgcttcctt gctcgcgccc tgggtgcggc cgagcagcga
      481 gccgaccttg tcgatgatct cggcgtgaaa ggcaccgagc gcgatgtgcg catcggcatg
      541 cctgggcgag agccggatcg tcgtctcgag cgccgtcttc accttcgcgc ccagcccctg
      601 cgccagcgcc ttggcgatcg agatgccctg gctgtaccgg ccgagcgcgt aggccatgca
      661 gtaccaggca ttggcatcct tggggtcctc gcgggtgcgc gcttcggcgc gttcggcaac
      721 ctcgtgcaac agcgcgagct tggtcttttc gctcttttcg agataggtcg cgtagatcgc
      781 ctgcgacttg ttggccaccg tcatgccggc accggcgccg gcggcatgcg ccttcaggcc
      841 cgcttcggtc gccttctgga actggccggc atgaaagagg gcccaggcgg ccagcaccga
      901 ttcgtccttg ggcaagggct cggcatcgcc ggcatgcagg cgcggccatt tcttcttcag
      961 cgccgcgatg tcatgggtgt acgtttcggc ggaatgggga aaggcggtcc acttggccat
     1021 gtcgggtcct tcagggtgaa gaaaggtagc gcgacgccag tgcgtcgagg tgctcccggc
     1081 ccgtcgcgtc gagatagggg cgaagcaggc acagcacgtg ataggccccg cgcagcaacg
     1141 cgggcccggc gctttcggcc tcgagcgccc ggcgcgggtt ctgcacgtat tcgaagctca
     1201 gccaccagct gaggacgacg accatcgccg ttgcgaccgg ctcgctgtcg cgccgctcga
     1261 ccgcgacgcc gccagtctgc gcgaggccgt cgagcacggc ctgcacggcc cgtgacgttc
     1321 gctgcagcac gaacgggaag tgcaactcga gcttgcggtt gttggcgagc aggtcgttga
     1381 ggtcgcggta gaggaagcgg tagcgccaga tcagctcgaa cagcaggtgg aagaacagcc
     1441 acgcgtcctc gatgtggccg acgtcctcgg cggcgcgcag cagctcgacc agtgccgcgt
     1501 cgtagcgctc gaagagccgg gtgacgagct cttccttggc cgggtagtgg tagtagagat
     1561 tgcccgggct gatgccgagc tcggcagaga tcagcgtcgt cgagacgttc ggttcgccga
     1621 agcggttgaa gagctcgagc gccgcctcga ggatccgctc ggcggtgcgc cgcggcttct
     1681 tcttcctgga ggcgctgggg gtggccatgg cggatcgtcg atgctgcgcc acgctagcca
     1741 gcgcggcctg gcctgtccat cggcgcgacc ccgatgccgg cgcgtcggtg gggcgctgcc
     1801 tacaatcgcg cccttcatgc cggccgtctc gttccagaac gtcgtcaagg tctacccttc
     1861 ctccggcggc ggcctgcgcg cgctcgacga cgtgagcttc gacgtcgagc cgggcgagtt
     1921 cttcgggctg ctcggcccga acggcgccgg caagacgacg ctcatcagca tccttgccgg
     1981 cctggcccgt gccacgtcgg gacgcatcgc ggtgctcggc cacgacgtcg ccggcgacta
     2041 cgcggcggcg cggcgccggc tcggcatcgt gccgcaggag ctggtcttcg atccgttctt
     2101 cacggtgcgc gaggcgctgc gcatccagag cgggtatttc ggggtgcacg gcaacgacgc
     2161 ctggatcgac gagcttcttc tcaacctcgg cctcgaggac aaggccgatg ccaacatgcg
     2221 ccagctgtcc ggcggcatga agcgacgcgt gctcgtcgcc caggccctgg tgcaccggcc
     2281 gccggtcatc gttctcgacg agccgaccgc cggcgtcgac gtcgagctgc gccagacgct
     2341 gtggcagttc gtcgcccgcc tcaacaagga gggccacacg gtgctcctga cgacgcacta
     2401 cctcgaggaa gccgaggccc tgtgcggccg catcgccatg ctcaagcagg gccgcatcgt
     2461 cgccctcgac cggaccaccg ccttgctcgc cggcacggcg agcacgatgc tgcagttcaa
     2521 gctcgaccgg ccgcttccgc cggcgctggc cgcgcatgcg cgcgtcaccg gccgcatcgt
     2581 ccagctgaag gcccacgatg cgcgcgaggt cgaggcgatc ctggcggcgt tgcacgccgc
     2641 cggttgcgag cccgaagacc tggagatcgg ccgggccgac ctcgaggacg tgttcatcga
     2701 gatcatgcac ggcgagacgc cggaacgggt cgcggcatga cgacgacgca ggtgggtgcg
     2761 gcgacggtcc gcgtcgagac cgagcacggc gtgctcgccg gcgcgcgaac cctgctctac
     2821 aaggaagtgc tgcgcttctg gaaggtgagc ttccagaccg tggccgcccc ggtgctcacc
     2881 gccgtcctct acctgctgat cttcggccac gtgctcgagg ggcgggtcaa ggtgttcggc
     2941 agcgtcggct acaccagctt tctcattccc ggcctggtga tgatgagcgt gctgcagaac
     3001 gcgttcgcca actcgagctc gtcgctgatc cagagcaaga tcaccggcaa cctcgtcttc
     3061 ctgctcgtca cgccgctctc gcactgggcc tggtttctcg cctacgtcgg cgcctcggtc
     3121 gttcgcggca tcgcggtggg caccggcgtg atgctggtga cgatctggtt cgcgccgctg
     3181 gacctcgcca acccgctgtg ggtgctcgtc ttcacgctgg ccggcgcgac gctgatgggc
     3241 gcgctcggcc tggtcgccgg tctctgggct gagaagttcg accagatcgc ggcgttccag
     3301 aacttcctga tcatgccgat gacgttcctt tcgggcgtct tctactccgt gcactcgctg
     3361 ccgccgacct ggatggcggt gagccacctg aatccgttct tctacatgat cgacggcttc
     3421 cgacgcggct tcttcggcac cagcgacgtc tcgccgtgga tcagcctcgt cgtcgtcggc
     3481 gcggccctcg tcctgatctg cggcctgtgc ctgcacctgc tcaagatcgg gtacaagatc
     3541 cgccattagg accgggaacg acgtgattcg acccgccacc acgacccggt cagctcgagg
     3601 tgatctgtac gcggagacgg gcggcggcgc ggagggcggc acccggcgcg gcctcatgcc
     3661 gtggcggtcg gcgctgcgcg ccgacttcac tgcgctgctc gcttcggggt cgcgccgcgt
     3721 cactcgcttc gttcgcttcg ctcactgcgc tcggacagac gcggcgagtc ggttcacgat
     3781 gcacgcgctg cgcgcgtgcc gaccccgaag ctgcgctgcc cggacgctcc tttgccggct
     3841 catgcccgac cgctccagcg aagcgccccc gcctgcccgg ccgctcgttt gccggctcat
     3901 ggcccaaccg ccccagcgaa gcgcaccggc tggcccggac gctcccttgc tcgctcgatc
     3961 tcggcgagtg cgtcttgcgt catccgacgg agaacgctga aatgacaacg ggcatgaaga
     4021 aaggcctcac cgcctacggc gacgccgagt tttccctttt cctgcgcaag gccttcatca
     4081 aggcgatggg cttgtcggac gacgcgctga accggccgat cgtcggcatc gccaacacct
     4141 tcagcgacta caacgcctgc cacggcaacg tgcccaggct cgtcgaggcc gtgaagcgcg
     4201 gcgtgatgct ggcaggcggc ctgccgatgg aatttccgac gatctcgctg cacgagtcgt
     4261 tcgcctttcc gacgagcatg ttcctgcgca acctgatggc gatggacacc gaggagatgc
     4321 ttcgcgccca gccgatggat gccgtggtgc tgatcggcgg ctgcgacaag acgctcccgg
     4381 cgcagatgat ggcggcggcc tcggccgacg tgccggcgat cgtcgtcccg gtcgggccga
     4441 tgctggtggg ccaccacaag ggcgagacgc tgggcgcctg caccgactgc cggcgcctgt
     4501 ggggcgagtt ccgggccggc cggatcgacg cggcggagat cgaggcgatc aacgggcgcc
     4561 tcgcgcccac cgcgggcacc tgcatggtca tgggcacggc gagcacgatg gcctgcatcg
     4621 tcgagacgat gggcctcggt ctggcgcagt cggcaacgat tcccgcgacc cacgccgatc
     4681 gccttcgctc ggccgaggcc agcgggcgtt gcgccgtggc gctggccgcc ggcaccgggc
     4741 ccaggccgag cgcgctgatg acgcgggcgg cgtttcgcaa cgcgctggtc gtgatgcagg
     4801 cgatcggcgg ctcgaccaac gcactcatcc atctcaccgc ggtcgccggt cggctcggcc
     4861 agcgcatcga tctcgacgag gtcgaccggc tcggccgcga ggttccggtg ctggtcgacc
     4921 tgaagccatc gggccggcac tacatggagc atttccactg ggccggcggc gtgccgcgcc
     4981 tgctgcacga gctcgccccc tggctcgacc tcgatgcgcc caccgtcgaa ggcgccaccc
     5041 tgcgcacgcg gatcgacggc atcgacaccg cgacgccaca ggacgtcatc cgcccgtgcg
     5101 ccaatccgat ccacgccggc ggcagcatgg cggtgctgcg cggcaacctg gcgccgcgtg
     5161 gcgccatcct caagcaatcg gcggcgagcc cggcgctgct ccagcacgag ggccgcgccg
     5221 tggtgttcga ttcgctcgag gacctcgccg cccggatcga ctcggccgat ctcgacgtcg
     5281 ccgccggcga cgtgctggtg ctgaagaacg cgggcccgat cggcgcaccc ggcatgcccg
     5341 aggccggcta tctgccgatc ccgaagaagc tcgccgcaca aggcgtgaag gacatggtgc
     5401 gcctctccga cgcgcgcatg agcggcaccg ccttcggcac gatcgtcctg cacatgacac
     5461 ccgaggccgc cctcggcggc ccgctggcgc aggtgcgaag cggcgaccgg atccgtctcg
     5521 acacggcggc acgccgcatc gatctgctcg tcgacgcggc ggagatcgag cgccgccgcg
     5581 aggagttcgt ccgcgtggga tcgcccggcg cggccgggag acccgatcgc ggttacgcgg
     5641 cgctcttcca ccgctcggtg ctgcaggccg acgaaggctg cgacttcgac ttcctggtgc
     5701 cgggtttcag ccgccgcgac tgaagtccgc agtattggcg gcaaagccta tcgcgggcgt
     5761 gatcaagaag gacgagatcc ttcgacgaac cgaagcactt tcaccgagct tcgagtcagg
     5821 tgtaagtcag agaagtcgag tgcacttgcg atcctcgtgc atcgattttg ctgcgccccc
     5881 ttgaacgagg ggtacttgcg agtgccttcc cccactacgc tcgccgcacc aaaccgtttt
     5941 gcttgttcat tcactcacag gaaatgggga aagcgatgcg tcatctcttc tctggccttc
     6001 tggcgagctt ggcactcggc gccgcagcgc tcctgtctcc ggtgacggcg agcgccgtca
     6061 ccgtggcgac catcggtggg tgttatgact gcggcgtgta cgacaccgct tcgctcatct
     6121 tcgacaacac gaccggtgga acacttacga acgcgcagat gctgctgacg gggtatcaag
     6181 gtcagaacaa cggtcagacc gcgactgtga atctcggcac gctgagcgcc ggcagcacgc
     6241 agatcttctg gggcaatctt cccggcgttt ccagcagcac gacccccaac aatctgacgg
     6301 cgtacgacta cgacgacgaa ttcatcaaca cgtcggccat catcaatgac ccgacgtgcg
     6361 gcggcggcgg ctgcgtcgac ggcggcggtc agcagtggta tgcgcaggtc gggaacttca
     6421 gcgtcacctt cactgcggtg gtgacgggcg gagcctacga cgggcaatcg gtgtattccg
     6481 tgttcagccc cacctcgaat gcgaccggtg gatttgtcgg atgggaaggg ctcgatccga
     6541 acggctactc ggaatcgccg cagtacgacg tgcacaccgg catcgtcacc ggccacttgg
     6601 ccgacatcga cctcggcttg ccgcccccac ctgtgcccga acccgaaacc tacgcgctga
     6661 tgctcgcggg tctcggcgcg ctcggcttcc tgcgccgtcg tggcgtcaag tcaccgcgcg
     6721 gctgatctcg agatgattgc gcaggagcgc ggccccggcg gcgcccctcg ctcgtatcgg
     6781 cggtgaactg cggctacgcc gtgggggctt agcgtccgcc ttccctcgct tccttcgagg
     6841 aaccggcgca gcgtggatcg gcggcacgct gcggtctgga agagggcatc tccgttcgct
     6901 gacgtcgccc gtcgcggccg ccggtcgggg tcaagtgccg cgcgtgcccg ggcggtcccg
     6961 ccgacggcga cgagagcgta tccttcccgg ctggcattct tcttcaccgg ttccatggcc
     7021 gaccccaccc ccgccgagat cgagcgcttc atcaccgacg gcctcgcctg cgagctcgtc
     7081 gaggtcgaag gcgacgggcg gcacttccac gccaccatcg tctccgccga attcgagggc
     7141 gcgaaccgcg tggggcgaca ccagcgcgtc tatcgcgccc tgggcgagcg catgcgcgag
     7201 cagatccacg cgctctccat gcagacgctg acccctgccg aataccgaaa ccgaccgacg
     7261 gcctgacgcg caagcgtccc tttgatctca tggacaaact ccgcatccgc ggcggccgcc
     7321 gcctcgccgg cgaggtgacg atttccggcg ccaagaacgc cgccttgccc gagctgtgcg
     7381 cggccctgct cacggccgag ccggtgctgc tttccaacgt gccgcgcctg cgcgacgtcg
     7441 cgacgatgct tcgcctgctg cgcacgatgg gcgtcaccgc cgaggaatcg aacgacgccg
     7501 aggcgagcgt ttcgctcgac gctcgcaaga tcgatcgtcg cgaggcggcc tacgacctcg
     7561 tcaagacgat gcgcgcgtcg atcctcgtcc tcgggccgct gctggcccgg ttcggcgagg
     7621 cgcgcgtgtc gctgccgggg ggctgcgcga tcggcacgcg cccggtcgac cagcacatca
     7681 aggggctgca ggcgctgggc gccgagatct cggtcgagca tggctacatc gtcgcccgcg
     7741 cgtcgcggct gaagggcgcg cgcatcacga ccgacatggt gacggtcacc ggcaccgaga
     7801 atttgctcat ggcggcgacg ctcgccgagg gcgagaccgt gctcgagaac gccgcgcaag
     7861 agcccgaagt cgccgacctg gcgcagatgc tgatcgcgat gggcgctcgc atcgagggcc
     7921 atggcaccag ccgcatccgc gtcgagggcg tgcgcgagct gcatggcacg tcgcatcgca
     7981 tcatcgccga ccgcatcgag gcaggcacct tcctgtgcgc cgtcgccgcg accggtggcg
     8041 atgtgctgct gcgccgggcc cgcgcggat
//
LOCUS       DASRWS010000405         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1308276, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000405 DASRWS010000000
VERSION     DASRWS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308276"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VFF72_07600"
     CDS             complement(<1..1503)
                     /locus_tag="VFF72_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93067.1"
                     /translation="MPIANRRIFWALAFIVALSAILSIGFVSYTELSTLANSSASVQR
                     SYDVLQTLDDLDAAITDSEANQRGFQLTRDPAYLTQYDAAVAQIRLAVSELRRLCADE
                     PSTATAVAELSRLANAQLDDLQKTVHASETGDRATLDHMVEGADTQIMAAFRRFSADL
                     DVIQRRLLEARRQEEQQARTTSEGVLVGTILLSLIFVGAAALISRRFDERRRQLEREI
                     AERRRAEEYREALLVSERAARAEAERATRLKDEFVATLSHELRTPLNAIVGWASILRN
                     DRRSASVAQGLEVIERNAKAQAQMIEDLLDMSRIVSGKLRIDLQLIDPAAVLKAAVAS
                     IRPSAAMKDINLHATLEPVGMIDSDAGRLQQIIWNLLTNAIKFTPPHGTVRVTLKPVD
                     AHVEMAVSDSGQGIAPEFLPYVFDRFRQADASTTRRHGGLGLGLSIVKSLVEMHKGTV
                     EARSDGEGQGSTFTVWLPLARSQPSSLFKPELAGEANELTLEGLRVLVVDD"
     gene            complement(1532..>2083)
                     /locus_tag="VFF72_07605"
     CDS             complement(1532..>2083)
                     /locus_tag="VFF72_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZV93068.1"
                     /translation="SWYGPTFHGERTSSGEPYDMYGMTAAHKTLPLPTYARVTNLKNG
                     RSVVVRINDRGPFVANRLIDLSYTAAAKLDMLREGTTLVEVRALVPGEPDTLTRASET
                     PPPTLYVQVGAFSDERNASRAVERLHAAGLATAFVRTPADGSKDRLFRVRVGPVTSVP
                     EFDQLAARLAKIGFRDARLANDD"
BASE COUNT          352 a          670 c          688 g          373 t
ORIGIN      
        1 gtcatcaacc acgaggactc gcaggccctc gagcgtcagc tcgttggcct cgccagcgag
       61 ctcgggcttg aagagcgaac tcggctgcga ccgcgcaagc ggcagccaga cggtgaaggt
      121 cgacccttgg ccttccccgt cactgcgtgc ttcgacagtg cctttgtgca tctccaccag
      181 gcttttcacg atcgacagac ccaggcccag gccgccgtgg cgccgcgtgg ttgaagcatc
      241 cgcctgccgg aagcggtcga acacataagg caggaactcc ggtgcgatgc cctgcccaga
      301 gtcgctgacc gccatctcga cgtgcgcgtc gaccggcttc agcgtcaccc gtaccgttcc
      361 atggggcggg gtgaatttga tggcgttggt gaggagattc cagatgatct gctgcagccg
      421 tccggcgtca ctgtcgatca ttcccacagg ctcgagcgtg gcgtgcaggt tgatgtcctt
      481 catagcggcg ctcggacgga tcgaagcgac ggccgccttc agcaccgcgg ctgggtcgat
      541 gagctgcaga tcgatccgca gcttcccgga gacgatgcgg ctcatgtcca ggaggtcctc
      601 gatcatctgt gcctgggcct tggcattgcg ctcgatgact tccaggccct gcgcaaccga
      661 ggcggagcgc cgatcattcc tgaggatgct tgcccagccc acgatggcgt tcaagggcgt
      721 tcgcagctcg tggctgagcg tggcaacgaa ctcatctttc aggcgggtgg cacgttcggc
      781 ctcagcgcgg gcggcgcgct cgctgaccaa gagggcttcg cgatactcct cggcccgccg
      841 ccgctcggcg atctccctct cgagctggcg gcggcgttca tcgaagcggc ggctgatgag
      901 ggcggcggca ccgacgaaga tcaagctcag gaggatcgtg ccgacgagca caccctcgct
      961 ggtcgtgcgg gcttgctgct cttcctggcg gcgcgcctcg agcaggcgcc gctggatgac
     1021 atccagatcg gcggaaaacc gccggaaggc ggccatgatc tgggtgtcgg ccccctcgac
     1081 catgtgatcg agcgttgcgc ggtcgccggt ttcgctggcg tgcacggtct tctgcaggtc
     1141 atcgagctgc gcattcgcca gccgcgacag ctcggcaacg gccgttgccg tcgagggctc
     1201 gtcggcgcac aggcggcgca actcactcac ggcgaggcgg atctgcgcca cggccgcatc
     1261 gtactgggtg aggtaggcag gatcgcgtgt cagctggaac ccgcgctgat tcgcctcgga
     1321 atccgtgatc gccgcgtcga ggtcgtcgag cgtctgcagg acatcgtagg aacgctggac
     1381 gctggcgctg gagttcgcca gggtggacag ctcggtgtag ctgacgaaac cgatcgaaag
     1441 gatcgcgctc aacgcgacga tgaacgccag tgcccagaag attcgccggt tcgcaatggg
     1501 cacgggtgcc tcgcgctgac tgcctcagtc gttagtcgtc gttggcaagc ctcgcgtcgc
     1561 ggaatccgat cttcgccagc cgggcggcga gctgatcgaa ttcaggaacg ctggtcacgg
     1621 ggccgacacg cactctaaag agccggtctt tgctcccatc ggcgggcgtg cgcacaaaag
     1681 ccgtggcgag accagccgca tgcagccgtt cgaccgcgcg gctcgcattc cgttcatccg
     1741 aaaacgcacc cacctgcaca taaagtgtcg gcggcggggt ttccgaagcg cgcgtcagag
     1801 tgtcgggctc gccaggcacc agcgcccgca cttccaccaa tgtggtgccc tcccggagca
     1861 tatcgagctt tgcggcggcc gtgtaagaca ggtcgatgag acgattcgcc acaaacggtc
     1921 cacggtcgtt gatgcgaacg accacgctgc gtccattctt cagattggtg acccgcgcgt
     1981 acgtgggcag cgggagagtc ttgtgagcag ctgtcattcc gtacatgtcg tacggctccc
     2041 cgctggaggt gcgctcccca tgaaacgtcg gcccatacca gga
//
LOCUS       DASRWS010000406         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_462867, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000406 DASRWS010000000
VERSION     DASRWS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462867"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="VFF72_07610"
     CDS             <1..1789
                     /locus_tag="VFF72_07610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZV93069.1"
                     /translation="LRACRLFVLSCVALAFGTAVQAASPALQSAPAATQAVQFEVFLP
                     LRHQAQLDALLLQLHDQNSPLYRHWLTPQEFTARFGADPDSVARVERALQRRGLVVDE
                     VGARSLSVSGTSAAVRSGLGLDLRSLQTARGMRVVAAHGAVLPAELTNEGAVVAAFRP
                     VVIHKRHGRRVALDSDLPQNRYGPTGPYWFTDIKQAYDMPSYKALTGKGVTIAIVMSN
                     DFLDSDMALYFGHEHLPVPRIVRVPVKGGAPFDPDGSFEVSLDIQQAGGMAPKATIRA
                     YNLPDLSDDSILRAYNRIVQHNAADIVSSSFGGAELFYTAAYNGGTDYTDILQIYESI
                     FKQGNAQGITFVASSGDSGGLGATTPDYFSGSPSAHFIPGVEHPAVSPHVTAVGGTNL
                     VTTNVAGSLQSRYVTENAYGDPELPYDPYGLGSNVSGGYWGSGGGESVIFAKPAYQYL
                     VPTHSTMRTVPDVSLQMGGCPLGISQLPCGTPRSASVVAVGGGLYGVIGTSVSAPGFA
                     GVLALFEQALGGQRLGNVNGLLYTEAALQSLSPGLGFFHRNIDGFNGYDYTARVYDRV
                     VGVGTPRVRNMILTPQLPAAGDPQSPSNP"
     gene            1838..>3235
                     /locus_tag="VFF72_07615"
     CDS             1838..>3235
                     /locus_tag="VFF72_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV93070.1"
                     /translation="MPGINLLDRVDGASLPRLRDAADVVALERVAYADRVAAQSTYEA
                     LQIGASHDPDAAAIRFLAAADPDETPLTISHRDFLGRVTQAANLFTSLGVGPGDVVSL
                     LLPLVPQSFAALFGAQAAGIANPVNPLLSADQIAHILQAAGAKVLVALGPMPGTDIWD
                     KVQRIREQLPQLAAILVVHGQADPARGIHDFDEAAAAQPADRLIGGRDIAAADVAAYF
                     HTGGTTGLPKLVRHTHGNEVYQAWALNAVLTCMNGVDLLFGLPLFHVGGALTQCLAPL
                     ANGGTLVVLSAAGWRNPKAIANVWRLVERYRPELFGGVPTVLGAAIQVPVGTADVSSI
                     RFVSGGGSAIPVAVGKTCMERLKVPVLEVYGMTETSSVHCLSYPGRPLRLGSVGHALP
                     YSRVRVVRLDAEGRPSGDCAVDEIGVVAMAGPGVFSGYVSEAHNRDAFIEPGWVNSGD
                     LGRLDADGYLWLTGRA"
BASE COUNT          458 a         1212 c         1057 g          508 t
ORIGIN      
        1 gcttcgagcc tgtcggctct tcgtcctttc gtgcgtcgcg cttgccttcg gcaccgccgt
       61 ccaggccgcc agccccgcgc tgcaaagcgc ccctgcggcg acgcaggcgg tccagttcga
      121 agtgttcctg ccgctacgcc accaggccca gctcgacgcg ctgctgctgc aactgcatga
      181 ccagaactcg ccgctgtatc gccattggct gacgccccag gaattcacgg cccgtttcgg
      241 cgccgacccc gacagcgtcg cccgcgtgga gcgcgccctg caacgacgcg gcctggtcgt
      301 cgacgaggtc ggcgcgcgca gcctgtcggt cagcggcacg agcgctgccg tgcgcagcgg
      361 cctgggcctc gacctgcgca gcctgcagac ggcgcgtggc atgcgcgtcg tcgccgcgca
      421 cggagcggtg cttcccgcgg agctgaccaa cgagggcgcc gtggtcgccg ccttccgccc
      481 tgtcgtcatc cacaagcgcc atggccgtcg cgtcgcgctc gacagcgacc tgccgcaaaa
      541 ccgctacggg ccgaccggcc cgtactggtt cacggacatc aagcaggcct acgacatgcc
      601 ttcatacaag gcgctgaccg gcaagggcgt gacgatcgcc atcgtcatgt ccaacgactt
      661 cctcgattcc gacatggcgc tctacttcgg ccacgagcac ctgccggtgc ccaggatcgt
      721 ccgcgttccg gtcaagggcg gggcgccgtt cgatcccgac ggatcgttcg aggtctcgct
      781 cgacatccag caggcgggcg gcatggcgcc gaaggcgacg atccgcgcgt acaacctgcc
      841 cgacctttcc gacgactcga tcctgcgcgc ctacaaccgg atcgtgcagc acaacgcggc
      901 cgacatcgtc agctcctcgt tcggtggcgc ggagctcttc tacaccgcgg cctacaacgg
      961 cggaaccgac tacaccgaca tcctgcagat ctacgaatcg atcttcaagc agggcaatgc
     1021 gcagggcatc accttcgtcg ccagctcggg cgacagcggc ggcctcggcg ccaccacgcc
     1081 cgactacttc agcggttccc cgtccgcgca tttcattccc ggcgtcgagc atccggcggt
     1141 cagcccgcac gtcacggcgg tcggcggcac caacctcgtc accaccaacg tcgccggctc
     1201 gctgcagtcg cgctacgtca ccgaaaatgc ctacggcgac ccggagctgc cctacgaccc
     1261 gtatggactg ggcagcaacg tgtcgggcgg ctactggggc tcgggtggcg gcgagagcgt
     1321 gatcttcgcc aagccggcct accagtacct cgtgccgacg cattcgacca tgcgcaccgt
     1381 acccgacgtg tcgctgcaga tgggcggctg cccgctcggc atttcgcaac tgccctgcgg
     1441 cacgccgcgc agcgcgtcgg tcgtcgccgt gggcggcggg ctctacggcg tgatcggcac
     1501 cagcgtctcc gcgccgggct tcgccggcgt gctcgccctt ttcgagcagg cgctcggcgg
     1561 ccagcgcctg ggcaacgtca acggccttct ctacaccgag gcggcactgc agtcgctgag
     1621 cccgggcctg ggcttctttc atcgcaacat cgacggcttc aacggctacg actacaccgc
     1681 gcgcgtctac gaccgcgtgg tgggcgtcgg cacgccgcgc gtacgcaaca tgatcctgac
     1741 gccgcagctg ccggcggccg gcgatccgca gtcgccgtcg aacccgtagg cctcgttcac
     1801 cgtcgacgcg ccggcggcct gccgagcaga ggacgccgtg ccgggcatca acctgctcga
     1861 tcgcgtcgac ggggcctcac tgccgcgtct tcgcgacgcc gccgacgtcg tcgcgctgga
     1921 gcgcgttgcc tacgccgacc gcgtcgccgc gcagagcacc tacgaggcgt tgcagatcgg
     1981 cgcatcgcac gatcccgatg ccgcggcgat ccgctttctc gccgccgccg atcccgacga
     2041 gacgccgctg acgatctcgc atcgcgactt cctcggccgc gtcacgcagg cggccaatct
     2101 cttcacgtcg ctcggcgtcg gaccgggcga cgtcgtcagc ctgctgctgc cgctcgtgcc
     2161 gcaaagcttc gcggcgctgt tcggcgcgca ggcggccggc atcgccaatc ccgtgaaccc
     2221 gctcctgtcg gccgaccaga tcgcgcacat cctccaggcg gccggggcca aggtgctggt
     2281 ggcgctgggc ccgatgccgg gcaccgacat ctgggacaag gtgcaacgca ttcgcgagca
     2341 attgccgcag ctcgcggcga tcctcgtcgt gcacggccag gccgatccgg cgcgaggcat
     2401 ccacgacttc gacgaggccg cggcggcgca gcccgccgat cggctcatcg gcggtcgcga
     2461 catcgccgcc gccgacgtcg ccgcctattt ccacaccggc gggacgaccg gcctgcccaa
     2521 gctcgttcga catacgcatg gcaacgaggt ctaccaggcg tgggcgctga acgccgtgct
     2581 gacgtgcatg aacggggtcg acctgctgtt cggcctgccg ctctttcatg tcggcggcgc
     2641 gctcacgcaa tgcctggcgc cgctggccaa cggcggcacc ctggtggtgt tgtcggcggc
     2701 cggctggcgc aatccgaagg ccatcgccaa cgtctggcgg ctggtcgagc gctaccggcc
     2761 cgaactattc ggtggcgttc cgaccgtgct cggggcggcg atccaggtcc ccgtcggcac
     2821 cgccgacgta tcgagcatcc gcttcgtctc cggcggcggc tcggcgattc ccgtggccgt
     2881 cggcaagacc tgcatggaac ggctgaaggt gccggtgctc gaggtctacg gcatgaccga
     2941 gacctcgagc gtgcattgcc tgagctatcc gggccggccg ctgcgcctgg gctcggtcgg
     3001 ccacgccctg ccgtacagcc gcgtccgcgt cgtccgtctc gacgccgaag gacgcccgtc
     3061 gggcgactgc gccgtcgacg agatcggcgt cgtcgcgatg gcgggcccgg gcgtgttctc
     3121 gggctacgtc agcgaggcgc acaaccggga cgccttcatc gagcccggct gggtcaactc
     3181 cggcgacctc ggtcgtctcg atgccgacgg ctacctgtgg ctcaccgggc gggcc
//
LOCUS       DASRWS010000407         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1309425, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000407 DASRWS010000000
VERSION     DASRWS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309425"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFF72_07620"
     CDS             complement(<1..561)
                     /locus_tag="VFF72_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZV93071.1"
                     /translation="MLAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDERDVILHIIEAVASQVFIPLTVGGGVRSVADVRRLLNAGADKVSF
                     NSAAVADPQVIRDASAKYGAQCIVVAIDAKRRAGGGDAWDVYTHGGRRNTGLDAVDWA
                     RRMAEAGAGEILLTSMDRDGTKSGFDL"
     gene            complement(657..1397)
                     /gene="hisA"
                     /locus_tag="VFF72_07625"
     CDS             complement(657..1397)
                     /gene="hisA"
                     /locus_tag="VFF72_07625"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZV93072.1"
                     /translation="MLLIPAIDLKDGRCVRLKQGDMSESTIFSEHPAAMARRWLEAGA
                     RRLHLVDLNGAFAGKPVNESAVKAILAEVGDEIPVQLGGGIRDLDTIERYLDDGLSYI
                     IIGTAAVKNPGFMRDACSAFGGHIIVGLDAKDGKVATDGWSKLTGHEVVDLAKKFEDY
                     GVEGIIYTDIGRDGMLTGINIDATVKLAQQLTMPVFASGGLSSIADIERLCAVQDVGI
                     EGVICGRAIYSGDLDFAAAQARADELAS"
     gene            complement(1471..2136)
                     /gene="hisH"
                     /locus_tag="VFF72_07630"
     CDS             complement(1471..2136)
                     /gene="hisH"
                     /locus_tag="VFF72_07630"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZV93073.1"
                     /translation="MSRRTVAVVDYGMGNLRSVSQAVARVARESGDFEVIVTSTPEDV
                     IAAERVVLPGQGAMPDCMRELRDSGLQEAVLDAAANKPLMGVCIGMQMLLERSEEGQG
                     PVGTPGLGLFAGEVVRFRLEGRTQPDGSRFKVPQMGWNQVVQASSHPLWAGIPDRSWF
                     YFVHSYCARPSDPRHSAGLTDYGGRFTSALARDNIFATQFHPEKSAEFGLALYRNFLH
                     WTP"
     gene            complement(2306..2923)
                     /gene="hisB"
                     /locus_tag="VFF72_07635"
     CDS             complement(2306..2923)
                     /gene="hisB"
                     /locus_tag="VFF72_07635"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZV93074.1"
                     /translation="MTTPTTPPVAARRATVQRDTKETKIRVSVDLDGSGAARLATGIG
                     FFDHMLEQIARHGLIDLEIEALGDLHIDGHHTVEDVGITLGQAIGQAVGDKRGLVRYG
                     HAYVPLDEALSRVVIDFSGRPGLTMDVPFKAGMIGGFDAQLTHEFFQGFANHALVTMH
                     IDNLRGENAHHQCETVFKAFARALRMAVAIDPRSAGVVPSTKGTL"
BASE COUNT          431 a         1003 c         1055 g          446 t
ORIGIN      
        1 caggtcgaac ccgctcttcg tgccgtcgcg atccatgctg gtgagcagga tctcgccggc
       61 gccggcctcg gccatgcgac gcgcccaatc gacggcatcg aggccggtgt tgcggcggcc
      121 accgtgcgtg tacacgtccc aggcgtcacc gccgcccgcg cgccgcttcg cgtcgatggc
      181 gacgacgatg cactgcgcgc cgtacttcgc cgaagcgtcg cgtatcacct gcgggtcggc
      241 gacggcggcc gagttgaagc tgaccttgtc ggcgccggcg ttgagcaggc gccgcacgtc
      301 ggccaccgag cggacgccgc cgcccaccgt gagcggaatg aacacctgcg acgcgaccgc
      361 ctcgatgatg tgcaggatca cgtcgcgctc gtcgctggtg gcggtgatgt cgaggaaggt
      421 cagctcgtcg gcgccctgct cgttgtagcg cgcggcgatc tccaccggat cgccggcgtc
      481 gcgaagctcg acgaagttga cgcccttgac gacgcgcccg gcggtgacgt cgaggcaggg
      541 aatgatgcgc ttggcgagca acggctaggc cgaaagagcg ccggccaggt gcccggccgt
      601 ggccgcggcc ggcggcgcgc ccccggacgg cggcgacgtg aggaagcgcg cgggtctcat
      661 gaggcgagct cgtcggcgcg ggcctgcgcc gccgcgaaat cgagatcgcc gctgtagatg
      721 gcgcggccgc agatcacgcc ttcgatgccg acgtcctgga cggcgcagag acgctcgatg
      781 tcggcgatcg acgacagccc gcccgaggcg aacaccggca tcgtcagctg ctgcgccagc
      841 ttcacggtgg cgtcgatgtt gatgccggtc agcatgccgt cgcggccgat gtcggtgtag
      901 atgatgccct cgactccgta gtcttcgaac ttcttcgcga ggtcgacgac ctcgtggccg
      961 gtgagcttgc tccagccgtc ggtggcgacc ttgccgtcct tggcgtcgag gccgacgatg
     1021 atgtggccgc cgaaggcgct gcaggcgtcg cgcatgaagc ccgggttctt caccgcggcc
     1081 gtgccgatga tgatgtagga aaggccgtcg tcgagatagc gctcgatcgt gtcgagatca
     1141 cggatgccgc cgcccagctg caccgggatc tcgtcgccga cctcggccag gatcgccttg
     1201 acggcgctct cgttgaccgg cttgccggcg aaggcgccgt tcaggtcgac caggtgcagg
     1261 cgccgcgcgc cagcctccag ccatcgccgc gccatcgccg cggggtgctc gctgaagatg
     1321 gtggattcgc tcatgtcgcc ctgcttgagg cgcacgcagc ggccatcctt caggtcgatc
     1381 gcagggatga gcagcatggc cgcatcgaac gaaggtgaac gcgacgaagg ggaatcacgg
     1441 ccgacgggtc ggcgagctcc ggatgcggcg tcaaggggtc cagtgcagga agttgcggta
     1501 cagggccaag ccgaattcgg cgctcttctc ggggtggaac tgggtcgcga aaatattatc
     1561 gcgtgccagc gccgaggtaa agcggccgcc gtagtcggtc aggcccgcac tgtggcgcgg
     1621 gtccgacggg cgggcgcagt agctgtgcac gaaatagaac cagctgcggt cggggatgcc
     1681 cgcccagagc gggtgcgacg acgcctggac cacctggttc cagcccatct gcggcacctt
     1741 gaaccggctg ccgtcgggct gcgtgcgccc ctcgagccga aagcggacga cctcgcccgc
     1801 gaagaggccc agccccggcg tgccgaccgg cccctggccc tcctcgctgc gctcgagcag
     1861 catctgcatg ccgatgcaca cgcccatgag cggcttgttc gcggcggcgt cgagcaccgc
     1921 ttcctgcaac cccgaatcgc gcagctcgcg catgcagtcc ggcatcgcgc cctgaccggg
     1981 caggacgaca cgttcggcgg cgatgacgtc ttcgggcgtc gaggtcacga tcacctcgaa
     2041 atcgccactt tccctggcga cgcgggccac tgcctgcgac accgagcgca ggttgcccat
     2101 gccgtagtcg accacggcga cagtgcgccg gctcatgcgc ccgcgccggc ggcgcgccgg
     2161 gccgccgcaa gcccggccgc ctcgcgtcga gagacggcgc accatttcgc gggcgcggcg
     2221 tcggccgggc gccgatccat cccaagcccg gccgcgccgc ctcggggggc agcgaacgtc
     2281 cccgtgagcg tgggggctcc gatcactaca gcgttccctt cgtcgacggc acgacgccgg
     2341 cggagcgcgg atcgatcgcc acggccatgc gcagcgcccg ggcgaacgcc ttgaagacgg
     2401 tctcgcactg gtggtgcgcg ttctcgccgc gcaggttgtc gatgtgcatc gtcaccaggg
     2461 catggttggc gaagccctgg aagaactcgt gggtgagctg cgcgtcgaag ccgccgatca
     2521 tgccggcctt gaacggcacg tccatcgtca ggccgggccg ccccgagaaa tcgatgacga
     2581 cccgcgacag cgcctcgtcg agcggcacgt aggcgtggcc gtagcggacc aggccgcgct
     2641 tgtcgcccac ggcctggccg atcgcctggc cgagcgtgat gccgacgtcc tcgacggtgt
     2701 ggtggccatc gatgtgcagg tcgccgagtg cctcgatctc gaggtcgatg aggccgtgcc
     2761 gggcgatctg ctcgagcatg tggtcgaaga agccgatgcc ggtcgcgagc ctggcggcgc
     2821 cgctgccgtc gaggtcgacg ctgacccgga tcttcgtttc cttggtgtcg cgctggacgg
     2881 tcgcgcgcct cgcggcgaca ggcggagtcg tcggggtggt catggcaggc tggcg
//
LOCUS       DASRWS010000408         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3053932, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000408 DASRWS010000000
VERSION     DASRWS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053932"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..317)
                     /locus_tag="VFF72_07640"
                     /pseudo
     CDS             complement(45..317)
                     /locus_tag="VFF72_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813239.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
     gene            complement(423..1784)
                     /gene="argA"
                     /locus_tag="VFF72_07645"
     CDS             complement(423..1784)
                     /gene="argA"
                     /locus_tag="VFF72_07645"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HZV93075.1"
                     /translation="MPTEQEPDIYSAQEAAEFAPAQFVRWFREVAPYVHAFRGKTFVV
                     AFGGELVRDGALNVLVQDLSLLSALGIRLVLVHGSLPQVNEQLRLKGYDMQFSRGLEP
                     TDGPALECAKEASGEIRLDIEAAFSQGLPNTPMSGSHIRVVSGNFVTARPAGVIDGVD
                     YHHTGLVRKIDTESIKFAIERGSAVVVLSPLGFSPTGDAFNLAMADLATNVAVSLRAE
                     KLIFLTDSPGVLDESGAVDTELARADADALLERGGLDEETAFYLKHASLAVKRGVARA
                     HLVPYGQDGSVLLEIFTHDGVGTMVVEDTLDDLRPATIDDVGAILSLIEPLEADGTLV
                     PRPRSVIERDVERFTVLEHDGVIYGCAALYPYKEELMAEMACLIVNPEWQGTGEGEIL
                     LRHMESRARAIGMKRIFVLTTRTSHWFIKRGFVQGGISDMPHAKQQHYNRSRNSMIFI
                     KKL"
     gene            1952..>2941
                     /locus_tag="VFF72_07650"
     CDS             1952..>2941
                     /locus_tag="VFF72_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZV93076.1"
                     /translation="MSEVPRPPGRAPRRTQEPRPERPIPAILYPEDLPVSGRRDEIAR
                     AIAANQVVIVCGETGSGKTTQLPKICLELGRGRRRMIGHTQPRRLAATSVAKRIAEEL
                     NTPLGEVVGYQVRFNDSTGPNASIKLMTDGILLAESQRDPLLKRYDTLIIDEAHERSL
                     NIDFLLGYLKQLLPRRPDLKLIITSATIDAERFASHFATDFAAGPPQDKAAPSEGSKP
                     APVIEVSGRLYPVELRYRPVQQLLSDDAAAAPQRKGSSNERLSGDEERDLIDAIVDAV
                     DECARQGPGDVLVFLPGEREIRESAEALRKHHPVGTEILPLYARLTQAEQEQIF"
BASE COUNT          495 a          992 c          977 g          477 t
ORIGIN      
        1 gcaggggcaa tgccccgtaa tgtcttttcg cagccgcgac gcgttcaggc cgaaggcggc
       61 acgaaccctt gcgcttcgac gctgccgtcc tcgaacagga acttctccat ttgctcgcgc
      121 aggtacttgc gcgcgcgcgc atcggccagg ttgagccggt tttcattgac caggcgtgtc
      181 tggacctgct tccactattc ccaggcgtct ttcgaaacct cgcgccaaat gcgcgtgccc
      241 agttcgccgg ggtagggcgg aaaatccagc ccttcggctt cgcgcttgag tttcacacag
      301 ttgatcgtac gggccatgat ttgccgccat tgaatgttga acgagactga tgattttagc
      361 ctgccctgaa agcgcaaaac cctgcgcggg gcagggcttt gcggcatgcg ggcgcggcgg
      421 cgtcagagct tcttgatgaa gatcatgctg ttgcgcgagc ggttgtagtg ctgctgcttg
      481 gcatgaggca tgtccgaaat gccgccctgc acgaagccgc gcttgatgaa ccagtgcgag
      541 gtgcgcgtgg tcagcacgaa gatgcgcttc atgccgatgg cgcgggcgcg gctctccatg
      601 tggcgcagca ggatctcgcc ttcgcccgtg ccctgccatt cggggttgac gatcaggcag
      661 gccatctcgg ccatcaactc ttccttgtag gggtagagcg ccgcgcagcc gtagatcacg
      721 ccgtcgtgct cgagcaccgt gaagcgctcc acgtcgcgtt cgatcacgct gcgcgggcgc
      781 ggcaccagcg tgccgtccgc ttccagcggt tcgatcaggc tgaggatggc gcccacgtcg
      841 tcgatggtgg ccgggcgcag atcgtccagc gtgtcctcga ccaccatggt gcccacgccg
      901 tcgtgagtga agatttccag cagcacgctg ccgtcctggc cgtagggcac caggtgggcg
      961 cgggccacgc cgcgcttgac cgcgagcgag gcatgcttga ggtagaaggc ggtttcctcg
     1021 tccaggccgc cgcgttcgag cagggcgtcc gcgtcggcgc gggccagctc ggtatcgacc
     1081 gcgccgctct cgtcgagcac gccgggcgaa tcggtcagga agatgagctt ttcggcccgc
     1141 agcgacacgg ccacgttggt ggccaggtcc gccatggcca aattgaaggc gtcccccgtg
     1201 ggcgagaagc ccagcggcga gagcaccacc accgccgagc cgcgctcgat ggcgaatttg
     1261 atcgactcgg tgtcgatctt gcgcaccagg cccgtgtggt gatagtcgac gccgtcgatc
     1321 acgcccgccg ggcgggccgt gacgaagttg cccgacacca cccggatgtg cgagcccgac
     1381 atgggcgtgt tcggcaggcc ctggctgaag gcggcttcga tgtccaggcg gatctcgccg
     1441 gaggcttcct tggcgcactc cagcgccggg ccgtcggtgg gctccagtcc gcggctgaac
     1501 tgcatgtcgt agcctttcag gcgcagttgc tcgttgacct gcggcagcga gccgtgcacc
     1561 agcaccaggc gtatgcccag cgccgacagc agcgaaagat cctgcaccag cacattcagg
     1621 gcgccgtcgc gcaccagctc gccgccgaaa gccaccacga aggtcttgcc gcgaaaggca
     1681 tgcacgtaag gcgcgacctc gcgaaaccat ctgacgaact gggcgggagc gaattcggcg
     1741 gcttcctggg cggagtagat gtcgggttcc tgttcggtgg gcatggtgcg cgggcgcggt
     1801 cgggcagagg gggaagggcg ggcctgacgc ccgccgccgg cgccgcgatg acggcgctgc
     1861 gcggaattat aggaccgcgg gaccgccggc acggccgggt cgttgcaagg cgaaacctat
     1921 aatgtcgcgc aatacgcaca acacgctctc catgtccgaa gttccccgtc cgcccgggcg
     1981 cgcgccgcgc cgcacccagg agccgcgccc cgagcgcccc attcccgcga tcctctatcc
     2041 cgaagacctg cccgtcagcg gccggcgcga cgagatcgcg cgcgccatcg cggcgaacca
     2101 ggtggtgatc gtttgcggcg aaaccggttc gggcaagacc acccagttgc ccaagatctg
     2161 cctggagctg ggccgcggac ggcgccgcat gatcggccac acccagccgc gccgcctggc
     2221 cgcgacgtcc gtggccaagc gcatcgccga ggaactgaac acgcccctgg gcgaggtggt
     2281 cggctaccag gtgcgcttta acgacagcac gggaccgaat gcgtcgatca agctgatgac
     2341 cgacggcatc ctgctggccg agtcgcagcg cgatccgctg ctcaagcgct acgacaccct
     2401 catcatcgac gaggcgcacg agcgcagcct gaacatcgat ttcctgctcg gctatctgaa
     2461 gcagctgctg ccgcgcaggc cggatctgaa gctcatcatt acctcggcca ccatcgacgc
     2521 cgaacgcttt gcctcgcatt tcgcgacgga ctttgccgcc gggccgcccc aggacaaagc
     2581 agccccctcg gagggcagca agcccgcgcc ggtgatcgag gtatcgggcc gcctgtaccc
     2641 ggtggagctg cgctaccggc ccgtacagca gctcctgtcc gacgacgcag ccgccgcgcc
     2701 gcaaagaaag ggttccagca acgagcgcct gtcgggcgac gaagagcgcg acctgatcga
     2761 cgccatcgtc gacgccgtgg acgaatgcgc gcgccagggc ccgggcgatg tgctggtgtt
     2821 cctgcccggc gaacgcgaga ttcgcgaatc ggccgaggcg ctgcgcaagc atcaccccgt
     2881 gggcaccgag atcctgccgc tctacgcccg cctgacgcaa gccgagcagg agcagatctt
     2941 c
//
LOCUS       DASRWS010000409         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3054224, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000409 DASRWS010000000
VERSION     DASRWS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054224"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /gene="metG"
                     /locus_tag="VFF72_07655"
     CDS             complement(<1..1914)
                     /gene="metG"
                     /locus_tag="VFF72_07655"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZV93077.1"
                     /translation="MAEKYFVTTALPYANGPFHIGHIMEYIQADIWVRFQRMQGNQVH
                     FVGADDAHGAPIMLKAEAENISPQELVGRIAAGRPKYLQGFHISFDHWHSTDSPENVA
                     LSQDVYRRLKAAGLIYSKPVEQFYDPVKGMFLPDRYIKGECPNCHSKDQYGDACEVCS
                     TVYSPTDLIEPYSVLTGARPILKTSDHLFFRLSDPKCVDFLNDWTTAPNRLQPQVVNK
                     AREWLSGKGDQALGDWDISRDAPYFGIPIPDAPGKYFYVWLDAPIGYLASLQSYFASG
                     KAKANGEPRSFEEFLAAPDTKQIHFIGKDIIYFHTLFWPAMLKFAGPPYKVPDNVYVH
                     GFITVSGEKMSKSRGTGISPLRYLDIGMNPEWLRYYIAAKLNGNVEDLDFNPDDFVAR
                     VNSDLVGKYVNIASRAANFITRHFDGALDYTGGTEALRRQAADLAQQVRDSYQTREFG
                     RAMREIMAYADRINQDFDARQPWVLAKDPARRAELQDVCSRTLYGFKLLSVLLAPVLP
                     EVGTRVARELFGLDRPFRWSDALEAPEHVAPYQHLMTRVDPKQLDALFESDSPAAAPK
                     TAGAPAAAAATGPAAGTPAATKTAAGSSASGSSTSSAASSASPSAGGEAASSAATISI
                     DEFKRLDLRVARIV"
     gene            complement(2056..>2485)
                     /locus_tag="VFF72_07660"
     CDS             complement(2056..>2485)
                     /locus_tag="VFF72_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZV93078.1"
                     /translation="MSIAVTAAEMGILVMGTLHTNGAAQTVDRMVNVFPSDKQSHVRT
                     MLSTSLRGVVSQQLLQRADKQGRVAALEILINTPAAANLIRQGKLDQLENTMQAGGQF
                     GMRTMDTAIQQLLDQRTITGKEAYKKAINKAKFELVKDIG"
BASE COUNT          397 a          761 c          845 g          482 t
ORIGIN      
        1 gacgatgcgc gccacgcgca ggtcgagccg cttgaattcg tcgatggaga tcgtcgccgc
       61 tgacgatgca gcctcgccgc cggcagaagg actcgcggaa cttgcggcgc tggacgtgct
      121 cgatccagat gcgctcgacc cggcggcggt ctttgtcgcc gcgggcgttc cggccgcagg
      181 gcccgttgcg gcggcggcgg ccggcgcccc tgccgtcttc ggagctgcgg cggggctgtc
      241 gctctcgaag agagcatcga gctgcttggg gtcgacgcgc gtcatcaggt gctggtaggg
      301 agctacgtgc tccggcgcct cgagcgcatc cgaccagcgg aacggccggt cgagcccgaa
      361 caattcccgg gcgacacggg tgccgacctc gggcagaaca ggcgccagca gcacgctcag
      421 gagcttgaag ccatagagcg tccgcgagca gacgtcctgc agctcggcgc gccgggcggg
      481 gtccttcgcc agaacccagg gttggcgtgc gtcgaagtcc tgattgatcc ggtcggcata
      541 cgccatgatc tcgcgcattg cgcggccgaa ttcgcgtgtt tggtagctgt cgcgcacctg
      601 ttgcgcgagg tccgcggcct gccggcgcaa cgcttcagtg ccgccggtgt aatcgagcgc
      661 accgtcgaag tggcgggtga tgaagttggc ggcgcggctg gcgatgttga cgtacttgcc
      721 gacgaggtcg ctgttgactc gggcgacgaa gtcgtcagga ttgaagtcta ggtcctcgac
      781 gttcccgttg agcttggcgg cgatgtagta gcgcagccac tcgggattca tgccgatatc
      841 gaggtagcgc agcgggctga tgcccgtgcc gcgggacttc gacattttct cgccggagac
      901 ggtgatgaag ccgtgcacgt agacgttgtc ggggaccttg tagggcggtc ccgcgaactt
      961 cagcatcgcc ggccagaaga gcgtgtggaa gtagatgatg tccttgccga tgaagtggat
     1021 ctgcttcgta tcgggcgccg cgaggaattc ctcgaagctg cgcggctcac cgttcgcctt
     1081 cgccttgccg ctcgcgaagt agctctgcag cgaggcgaga tagccgatcg gggcatcgag
     1141 ccagacatag aagtacttgc ccggagcgtc ggggatcgga atgccgaagt acggcgcgtc
     1201 gcgcgagatg tcccagtcgc cgagcgcctg gtcgcccttg cccgaaagcc attcgcgtgc
     1261 tttgttcacc acctgcggct gcagtcgatt cggtgcggtg gtccagtcgt tcaggaagtc
     1321 gacgcatttc ggatcggaga gccggaagaa gagatggtcc gaggtcttga gaatcggcct
     1381 cgccccggtg agcaccgagt acggctcgat gagatcggtc ggcgagtaca cggtgctgca
     1441 gacctcgcag gcatccccgt actggtcctt ggaatggcag ttggggcatt cacccttgat
     1501 gtatcggtcc ggcaggaaca tgcccttgac cgggtcgtag aactgctcga cgggcttcga
     1561 gtagatgaga cccgccgctt tcaggcggcg atagacgtcc tgcgacaacg ccacgttttc
     1621 cggcgagtcc gtcgagtgcc agtggtcgaa actgatatgg aagccctgca gatattttgg
     1681 ccgcccggcc gcgatccgcc cgacgagctc ctgcgggctg atgttctccg cctccgcttt
     1741 cagcatgatc ggcgcaccgt gcgcgtcatc ggcaccgaca aaatgcacct gattgccctg
     1801 catgcgctgg aaccgcaccc agatatccgc ctggatgtat tccatgatat gcccgatgtg
     1861 gaacgggcca ttggcgtagg gcagcgccgt cgtgacgaaa tatttctcgg ccatcgcggc
     1921 agtttacagg agcgccccgc cggcgacagc tcgtcgagcc ggctgcgcgg ccggccgcct
     1981 accggcgcag tccttggtcg tccagggcga cttatcgagc ccttgctggc gcttcacttc
     2041 ctcgagattc gggcttcagc cgatgtcctt gaccagctcg aacttcgcct tgttgatggc
     2101 ttttttgtag gcctccttgc cggtgatcgt ccgctgatcg agcagctgtt ggatggcggt
     2161 gtccatggtg cgcatgccga actggccgcc ggcctgcatc gtgttctcga gctggtcgag
     2221 cttgccctgg cggatcaggt tcgcggcggc gggcgtgttg atcaggatct cgagtgccgc
     2281 cacacggccc tgcttgtcgg cgcgctgcag cagctgctgc gagacgacgc cgcgcagcga
     2341 cgtcgaaagc atcgtccgca cgtgcgactg cttgtcggac ggaaagacat tgaccatgcg
     2401 gtccacggtc tgggcggcgc cgttcgtatg cagcgtgccc atgacgagaa tgcccatctc
     2461 cgccgcggtg accgcgatgc tcatg
//
LOCUS       DASRWS010000410         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4195553, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000410 DASRWS010000000
VERSION     DASRWS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195553"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..486)
                     /locus_tag="VFF72_07665"
     CDS             complement(160..486)
                     /locus_tag="VFF72_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZV93079.1"
                     /translation="MGYYERHIFFCLNDRDNGENSCAHHGAKAAFDHCKSRVKKEHLA
                     GPGKVRVNKSGCLDRCAGGPVAVVYPEGTWYSYVDEADIDEIVDSHLKKGTVVERLLL
                     PPDVGR"
     gene            complement(497..1609)
                     /locus_tag="VFF72_07670"
     CDS             complement(497..1609)
                     /locus_tag="VFF72_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZV93080.1"
                     /translation="MPLTWLYVALIVYASLYPFVGWRVPGVAPFHFMVLGWPHWWTWF
                     DLVSNLLGYMPLGFLLFVALIRGGRATATAAGLASIFGTLLSFAMETLQNYLPQRVAS
                     NVDLALNAAGTILGVAIGVMLQWQGGVERWQKLRERWFVTRSAGGMAMLVLWPVALLF
                     PTAVPFGLGHVLGRLQPLFADWLQGTAAEGLTAGWTLAAARRAQAVQLSPGSELAIIV
                     LGLLAPCLIAFTISTPGWRRAALAVGAAAIGCATTTLSTTLNFGPQHALAWMTPFAAQ
                     GVLVGVAAAVLLSLVPSRIAAGFGLIALTALVMLVAQAPADPYFEESLSGWEQGRFIR
                     FHGAAQWVGWAWPYAGLTYLLARLAAREPRRPGSAF"
     gene            complement(1748..>2131)
                     /locus_tag="VFF72_07675"
     CDS             complement(1748..>2131)
                     /locus_tag="VFF72_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZV93081.1"
                     /translation="FAGLFYLPRIFINLRQVPADSHAERERLLLMARKLYRFSTGLMV
                     VAIALGLVLWLGYGIGKGPHNHWLDAKIAFVVIAIGYHHMCGGLLRRFEAFANVRSDR
                     WLRVFNEMAVVLFTVIVVLVAVKPF"
BASE COUNT          388 a          731 c          732 g          280 t
ORIGIN      
        1 cgagcgtctg cgcgaccttg ttgtcgagcg tgccgccacc ttgcggatgg ggatgacaaa
       61 tgacggcgac tccaaccggc gcggcttcgg gccgatcgat cgcgcattcg atccggccgg
      121 ccggcccgtc ggtccattcg cggaccgtgc gcgggttcat caacggccga cgtcgggtgg
      181 caggagaagc cgctcgacga ccgttccctt cttcaggtgc gagtcgacga tctcgtcgat
      241 gtcggcctcg tcgacatagc tgtaccaggt gccttcgggg tagaccacag cgaccgggcc
      301 gccggcgcag cggtcgaggc agcccgactt gttgaccctc accttgcccg gcccggcgag
      361 atgctccttc ttgacgcgcg acttgcagtg atcgaaggcc gccttggcgc cgtgatgggc
      421 acacgagttc tcgccgttgt cgcgatcgtt caggcaaaag aagatgtgcc gctcgtagta
      481 gcccatcgcc aagattctag aaggccgatc ccggtcgccg gggctcgcgc gcggccaacc
      541 gcgcgagcag gtaggtcaag cccgcatacg gccacgccca gccgacccat tgggcggcgc
      601 cgtggaagcg gatgaaccgg ccctgctccc agccgctcag gctttcctcg aaatacgggt
      661 cggccggcgc ctgcgccacg agcatgacca gcgcggtcag ggcgatcagg ccgaaaccgg
      721 cggcgatgcg gctgggcacc aggctcagca gcaccgccgc ggccacgccg acgaggacgc
      781 cctgcgcggc gaacggcgtc atccacgcca gcgcgtgctg cggcccgaaa ttgagcgtcg
      841 tcgacaacgt ggtcgtggcg cagccgatcg ctgccgcgcc gacggccagc gccgcgcgcc
      901 gccagccggg cgtcgagatc gtgaaggcga tgaggcaagg cgcaagcagg ccgaggacga
      961 tgatcgcgag ctcgctgccc ggcgaaagct gcaccgcctg cgcccgtctg gcggcggcga
     1021 gcgtccagcc ggcggtcagg ccttctgcgg ccgtgccctg cagccagtcg gcgaacaagg
     1081 gctgcagccg tccgaggaca tggccgaggc cgaacggcac cgcggtcgga aagaggagcg
     1141 cgaccggcca cagcaccagc atcgccatgc ccccggcgct tcgcgtgacg aaccagcgtt
     1201 cgcgcagctt ttgccagcgc tcgacgccgc cttgccactg cagcatcacg ccgatcgcca
     1261 cgccaaggat cgtgcccgcc gcgttcagag ccagatcgac gttggacgca acccgctgcg
     1321 gcaggtagtt ctgcagcgtc tccatcgcga acgagagcag ggtgccgaag atcgacgcca
     1381 ggccggcggc ggtggccgtc gcacggccac cgcgaatgag cgccacgaac agcagaaagc
     1441 cgagcggcat gtagccgagc aaattcgaga cgaggtcgaa ccacgtccac cagtgcggcc
     1501 agccgagaac catgaagtga aacggcgcga cgcccggcac ccgccagccg acgaagggat
     1561 acaggctcgc gtagacgatc agtgcgacgt acagccacgt caggggcacg gccaggctgc
     1621 gatgtcgcga cactcgacgc ctcagcgacg cgggcaaatc ggctgccgac gcggtcgcgt
     1681 cacgaacgcg cctcgggctt cggcacgggc ccgcgtcacg acgatcccgc tggccgacgg
     1741 cgcatggtca aaagggcttg accgcgacca gcacgacgat cacggtgaac agcacgacgg
     1801 ccatctcgtt gaagacgcgc agccagcggt cgctgcgcac gttcgcgaac gcctcgaacc
     1861 tgcgcagcag gccgccgcac atgtggtggt agccgatggc gatgacgacg aatgcgatct
     1921 tcgcgtcgag ccaatgattg tgcggaccct tgccgatgcc gtagcccagc cacagcacga
     1981 gcccgagggc gatcgccacg accatcaggc cggtcgagaa ccggtagagc ttgcgcgcca
     2041 tgagcagcag ccgctcgcgc tcggcgtggc tgtcggcggg tacctggcga aggttgatga
     2101 agatgcgcgg caggtagaag aggccggcga a
//
LOCUS       DASRWS010000411         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4196413, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000411 DASRWS010000000
VERSION     DASRWS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4196413"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFF72_07680"
     CDS             complement(<1..200)
                     /locus_tag="VFF72_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93082.1"
                     /translation="MTPNASESGQDDDPSPPDESFALPVPDLAHWRGGNAGVEGVWSF
                     DGRDAGAEVLVTALVHGNELCGA"
     gene            315..446
                     /locus_tag="VFF72_07685"
     CDS             315..446
                     /locus_tag="VFF72_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93083.1"
                     /translation="MQSRFDAAALDPTRPIRAGTGRISGLLLSIALRGAMRLVAVFA"
     gene            483..734
                     /locus_tag="VFF72_07690"
     CDS             483..734
                     /locus_tag="VFF72_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93084.1"
                     /translation="MQPSEPMRLDEPGTTFPYVASTADWKRRFAGRLLEIDSDRDPAA
                     LLHVADDLALSSHWRLMAPEEAAETLFAEVVPLRRRDAG"
     gene            complement(744..1706)
                     /locus_tag="VFF72_07695"
     CDS             complement(744..1706)
                     /locus_tag="VFF72_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HZV93085.1"
                     /translation="MPPFKAVTYSSPAAGVRLIVLGAVHGNETCGTRAIERLMVELDA
                     GRKVLASGRLTLVPVTNAMAYAAGRRAGDRNLNRRLAPTDAPREFEDHVANWLCPLLA
                     GNEVLLDLHSFSAPGTPFAFIGPADNTGPLEPFAQAAREEALALRLGVGRAVDGWLDT
                     YARGVARRREIADRFPGAALDLDPRYGIGTTEYMRSVGGAAVTLECGRHDDAQAPEVA
                     YRAIVATLAHLRMIEAPDPSPAAAMESLRLCEVVDKLDAGDAFVREWRSFDAVGAGET
                     IGSRRDGATVAAPFDGFIVFPNAGSDAGKEWFYLARATDRLRRA"
     gene            1804..2226
                     /locus_tag="VFF72_07700"
     CDS             1804..2226
                     /locus_tag="VFF72_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZV93086.1"
                     /translation="MLAQAQTTLAIDTPGRGLVEITRPLAEWVASTRLRTGLVTAFVR
                     HTSASLVIQENADPDVRGDLDRFLARLVPDGDALFRHRDEGPDDMPAHVRAALTAVQV
                     SIPLAEGRPVLGTWQGLYLWEHRLRPHRREVVLHLIGE"
     gene            complement(2294..3295)
                     /locus_tag="VFF72_07705"
     CDS             complement(2294..3295)
                     /locus_tag="VFF72_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZV93087.1"
                     /translation="MATLTLDEARRRVAATLVAAGASAAMAESTARALVLAEAQGLGS
                     HGLSRVAQYATHLRNGRVDGSALARVARSRGAVAVVDAGEGLAFPACELAVVEASRRA
                     AEFGIGFVGVTRSHHAGVMVDHLRPVAETGLVGLAFTNSPAAMPAAGGRHAIFGTNPI
                     AAIFPRRGAAPLVIDLSLSEVARGKVMVAAKEGRPIPEGWALDAQGLPTTDAKAALAG
                     SMLAIGAVSSSKGAMLALVVELLVTALIGANFGFEASSFFVDEGNRPGLGQAFLALDP
                     GALAGREQFLDRLEVLVREMLHDDGVRLPGARREALAARAREGGISVNDATLATLVP"
     gene            complement(3295..4221)
                     /locus_tag="VFF72_07710"
     CDS             complement(3295..4221)
                     /locus_tag="VFF72_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HZV93088.1"
                     /translation="MARVVICEFMDAPGIEALRARHDVLYDSGLVDDAPRLRALAADA
                     EALVVRNRTQVRGDLLAALARCKVVGRLGVGLDNIDVEACAARGIAVIPAVGANARSV
                     AEYVLASAMLLRRGVYGATADVVAGRWPREALSNGREIGGTTLGLVGFGSIGRVTAAL
                     AQGMGVRVIAHDAAVPDTDPVWTTSGVECTTLERVVADADIVSLHVPLLPSTRGLFDA
                     RRIAAMKPGAILVNAARGGIVDEAALAAALKSGHLGGAALDTFGVEPLPSANAFADCP
                     NLLLTPHVAGPSKESSARISTLIATRVMEALA"
     gene            complement(4273..5448)
                     /locus_tag="VFF72_07715"
     CDS             complement(4273..5448)
                     /locus_tag="VFF72_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HZV93089.1"
                     /translation="MSVIDKNTTFLGYRRENGRVGVRNHVVILPLDDLSNAASEAVAN
                     NIKGTLAIPHPYGRLQFGADLDLHFRTLIGAGSNPNVAAAVVIGIEDGWTQKVVEGIR
                     ATGKPVVGFGIELHGDHDTIMRASKAAREYVQWASEKQREACPISDLWVSTKCGESDT
                     TSGCGSNPTVGNAFDKLHPLGATLCFGETTEITGGEQIVAARCANDAVRERFMFMFDR
                     YQDVINRHKTSDLSDSQPTKGNIAGGLTTIEEKALGNIQKIGKKCTVDGVLDKAETPT
                     HPGLWFMDSSSAAAEMVTLCAASGFAVHLFPTGQGNVIGNPIVPVIKLCANPRTVRTM
                     SEHIDVDTSGLLQREMNLDEAGDKLLECMLRTVNGRFTAAEALGHREFVLTRLYESA"
     gene            complement(5450..5731)
                     /locus_tag="VFF72_07720"
     CDS             complement(5450..5731)
                     /locus_tag="VFF72_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HZV93090.1"
                     /translation="MIHCVLHDAEDTVAVVVVEGLKAGTAMTGWIMDEDRTIGLEARQ
                     DIPIGHKVALKDMQAGDTVIKYGIDMGKVVAPIKKGEHAHVHNIKTKRW"
     gene            5832..>6031
                     /locus_tag="VFF72_07725"
     CDS             5832..>6031
                     /locus_tag="VFF72_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV93091.1"
                     /translation="MTKPPVPQSFGRLSPDVAGMPLYRAVERDLLGAIESSRFGPGDA
                     LPSEADLAASFGVSVGTVRKAV"
BASE COUNT          868 a         2154 c         2108 g          901 t
ORIGIN      
        1 gcaccgcaca gctcgttgcc atggacgagc gcggtgacga gcacttcggc gccggcatct
       61 ctgccgtcga aggaccacac accttcgacg ccggcgttgc cgccgcgcca atgagcgagg
      121 tccggcaccg gcaacgcgaa cgattcgtcc ggcggcgatg gatcgtcgtc ctggccggac
      181 tcgctggcgt tcggggtcat gccgatcagc atagagcgcc gcgacattca cgcaccgcaa
      241 agctccgtga aggattgccg cagcgacgtc gtcgacacac ccccgccgcc tcgccggcac
      301 cgtacattgc cggcgtgcaa agccgcttcg acgccgccgc cctcgacccc acccggccca
      361 tccgtgccgg caccggccgg atctcgggcc tgctgctgtc gatcgcgttg cgcggcgcga
      421 tgcggctggt ggccgtcttc gcgtagcgcg acgcggcctt cgacgacacg ccgccttccg
      481 ccgtgcagcc atcggagccg atgcgcctcg acgagccggg caccaccttt ccctacgtcg
      541 ccagcaccgc cgactggaag cgccgcttcg ccggcaggct gctcgagatc gacagcgacc
      601 gcgatcccgc ggcgctgctg cacgtcgccg acgacctcgc gctcagcagc cactggcgac
      661 tgatggcgcc cgaagaagcg gcggaaacac tgttcgccga ggtcgtgccc ctgcggcgac
      721 gcgacgccgg ctgagcgccg ccgtcaggcg cggcgcagtc ggtcggtcgc gcgggcgagg
      781 tagaaccact ccttgcccgc gtcgctcccg gcgttgggaa agacgatgaa gccgtcgaag
      841 ggcgccgcca cggtcgcgcc gtcgcggcgc gagccgatgg tctcgccggc gccgaccgcg
      901 tcgaagcttc gccactcgcg aacgaacgca tcgccggcgt cgagcttgtc gacgacctcg
      961 cacaggcgca ggctctccat cgccgcagcc ggcgacggat cgggtgcctc gatcatccgc
     1021 aggtgcgcga gggtcgcgac gatggcccga tacgcgacct cgggcgcctg cgcatcgtcg
     1081 tggcggccgc attcgagagt caccgccgcg ccgccgaccg agcgcatgta ttcggtcgtg
     1141 ccgatgccgt agcgcggatc gagatcgagc gccgcgcccg gaaagcggtc ggcgatctcc
     1201 cgccgccggg cgacgccgcg cgcataggta tcgagccagc cgtcgacagc ccggccgacg
     1261 ccgaggcgca gtgccagtgc ctcctcgcgc gcggcctgcg cgaagggctc gagcgggccg
     1321 gtgttgtcgg cgggcccgat gaaggcgaac ggcgtgccgg gcgcggaaaa ggaatgcagg
     1381 tcgaggagaa cctcgttgcc ggcgagcaag ggacacagcc agttggcgac gtggtcctcg
     1441 aactcgcgcg gcgcatcggt cggcgcgagc ctgcggttga ggttgcgatc gccggcgcgc
     1501 cggccggccg cgtaggccat cgcgttggtc accggcacca gcgtgagccg gccgctggcc
     1561 aggaccttcc ggccggcatc gagctcgacc atcaagcgct cgatggcgcg ggtgccgcag
     1621 gtctcgttgc cgtgaaccgc tccgagcacg atgaggcgca cgcccgctgc ggggctcgaa
     1681 taggtgacgg ccttgaaggg cggcatcgac tggcgaccgg gagatcgctc gaaaggcgcg
     1741 gtgcgcggga aggcgattct agggaggctg ccctccctcg cccgccgcga ctatcctcgc
     1801 cgcatgctcg cgcaggcaca gaccacgctg gccatcgaca cgccgggccg cggcctcgtc
     1861 gagatcactc gccctctggc cgaatgggtc gcgtcgacgc gcctgcgcac cggcctcgtg
     1921 accgccttcg tgcgccacac ctccgccagc ttggtgatcc aggaaaacgc cgaccccgac
     1981 gtgcgcggcg atctcgatcg cttcctcgca cgcctggtgc ccgacggcga tgccctgttc
     2041 cgccaccgcg acgaagggcc ggacgacatg cccgcacacg ttcgcgccgc gctcaccgcg
     2101 gtgcaggtgt cgatcccgct ggccgagggg cgaccggtcc tcggcacctg gcagggcctg
     2161 tatctctggg agcatcggct gcgaccgcat cgccgcgagg tggtgctgca tctcatcggc
     2221 gagtgagggg gccgcgccgc ggcgggcgag gcgcgcaacg gcacatgtgc cgccagcctg
     2281 cgcctgcgcg aaatcaggga acgagggtcg ccagcgtcgc gtcgttcacg gaaatgccgc
     2341 cttcccttgc ccgggccgcc agcgcctcgc gccgcgcgcc gggaagccgc acgccgtcgt
     2401 cgtgcagcat ctcgcgcacc agcacttcga gccggtcgag aaactgctcg cggcccgcca
     2461 gcgcgccggg gtcgagggcg agaaaggcct ggccgaggcc ggggcggttg ccctcgtcga
     2521 cgaagaagct cgacgcctcg aagccgaaat tcgcgccgat cagtgccgtg accagcaact
     2581 ccacgaccag ggcgagcatc gcccccttgc tgctcgagac cgcgccgatg gcgagcatcg
     2641 agccggccag agccgccttg gcgtccgtcg tcggcaggcc ctgcgcatcg agcgcccagc
     2701 cttcgggaat ggggcgaccc tccttggccg cgaccatcac cttgccgcgc gccacttcgc
     2761 tcaggctcag gtcgatgacg agcggcgccg cgccgcgccg cggaaagatc gcggcgatgg
     2821 gattcgtgcc gaagatggcg tgccggccgc cggccgcggg catcgccgcc ggcgagttgg
     2881 tgaaggccag gccgaccagc ccggtctcgg ccaccggccg cagatgatcg accatcacgc
     2941 cggcgtgatg gctgcgcgtc acgccgacga agccgatccc gaattcggcg gcccttcgcg
     3001 acgcttcgac aacggcaagc tcgcaggccg ggaacgcgag gccttcgccg gcatcgacca
     3061 cggcgaccgc ccccctgctg cgcgcgactc gcgccagcgc actgccgtcg acgcggccgt
     3121 tgcgcaggtg cgtcgcgtat tgcgcgacgc gcgaaaggcc gtgcgagccc aggccctgcg
     3181 cctcggcgag caccagcgcg cgtgccgtcg actcggccat cgccgcgctc gcgccggctg
     3241 ccacgagcgt ggcggcgacg cggcgccggg cctcgtcgag cgtgagcgtg gccatcaggc
     3301 gagcgcctcc attacccgcg tcgcaatcag cgtcgagatg cgcgcgctcg actccttcga
     3361 ggggcccgcg acatgcggtg tcagcaacag gttggggcaa tcggcaaagg cattggcaga
     3421 gggcaacggc tcgaccccga aggtgtcgag cgcggcgccg ccgaggtgac cgcttttcag
     3481 cgccgcggcc agggcggcct cgtcgacgat gccgccgcgc gccgcgttga cgaggatcgc
     3541 ccccggcttc atggcggcga tgcgccgggc atcgaacagg ccgcgcgtcg atggcagcag
     3601 cggcacgtgc aggctcacga tgtcggcgtc ggcgacgact cgctcgagcg tcgtgcattc
     3661 gacgccgctc gtcgtccaga ccgggtcggt gtcaggaacg gccgcgtcgt gggcgatcac
     3721 gcgcacgccc atgccctgcg ccagcgcggc ggtgacgcgg ccgatcgagc cgaagccgac
     3781 cagcccgagc gtcgtgccac cgatctcgcg gccgttcgag agcgcctcgc gcggccagcg
     3841 gccggcgacg acgtcggcgg tcgcaccgta gacgccgcgg cgcagcagca tcgccgaggc
     3901 gaggacgtat tcggcgaccg aacgcgcgtt cgcgccaacc gccgggatga cggcgatgcc
     3961 acgcgccgcg caggcctcga cgtcgatgtt gtcgaggccg acgcccagcc ggccgacgac
     4021 cttgcagcgt gccagcgccg cgagcagatc gccgcgcacc tgggtgcggt tgcgaacgac
     4081 gagcgcttcg gcgtcggccg ccagggcgcg caggcgcggc gcgtcgtcga cgaggccgga
     4141 gtcgtagagc acgtcgtggc gcgcgcgcag cgcctcgatg cccggcgcgt ccatgaattc
     4201 gcagatgacg acgcgggcca tcagcgtggc ccggtggccg ggccgaccgg cccggagcac
     4261 gccacgctca tgtcacgccg actcgtagag ccgcgtcagc acgaactcgc ggtggccgag
     4321 cgcttcggcc gcggtgaaac ggccgttgac ggtgcgcagc atgcattcga gcagcttgtc
     4381 gccggcttcg tcgaggttca tttcgcgctg cagcaggccc gacgtgtcga cgtcgatgtg
     4441 ctcgctcatg gttcgcacgg tgcgcgggtt ggcgcacagc ttgatcaccg ggacgatcgg
     4501 gttgccgatg acgttgcctt gccccgtcgg gaacagatgc accgcgaaac ccgacgccgc
     4561 gcacaacgtg accatctcgg cggcggcgct cgacgaatcc atgaaccaca ggcccgggtg
     4621 ggtcggcgtc tcggccttgt cgaggacgcc gtcgacggtg cacttcttgc cgatcttctg
     4681 gatgttgccg agcgccttct cctcgatcgt cgtcaggccg ccggcgatgt tgcccttggt
     4741 cggctgcgag tcggacaggt cgctcgtctt gtgccggttg atgacatcct ggtagcggtc
     4801 gaacatgaac atgaagcgct cgcgaaccgc gtcgttggcg cagcgcgcgg cgacgatctg
     4861 ctcgccgccg gtgatctcgg tcgtttcgcc gaagcacagg gtggcgccga gagggtgcag
     4921 cttgtcgaag gcgttgccga cggtcgggtt ggagccgcac cccgacgtgg tatcgctctc
     4981 gccgcacttg gtggagaccc agaggtcgga gatcgggcag gcctcgcgct gcttctcgct
     5041 cgcccactgc acgtactcgc gcgccgcctt cgaggcccgc atgatggtgt cgtggtcgcc
     5101 gtgcagctcg atgccgaagc ccaccaccgg cttgccggtc gcacggatgc cctcgaccac
     5161 cttctgcgtc cagccgtcct cgatgccgat gacgacggcg gcggcgacgt tcgggttgct
     5221 gccggcgccg atcagggtgc ggaagtgcag gtcgaggtcg gcgccgaact gcagccgccc
     5281 gtacgggtgc gggatcgcca gcgtgccctt gatgttgttg gcgacggcct ccgaggccgc
     5341 gttcgacagg tcgtcgagcg gcaggatgac gacgtgattg cgcacgccga cgcggccgtt
     5401 ctcgcggcga tagccgagga atgtggtgtt cttgtcgatc acgctcatct caccacctct
     5461 tcgtcttgat gttgtggacg tgcgcgtgct cgcccttctt gatcggcgcc acgaccttgc
     5521 ccatgtcgat gccgtacttg atgacggtat cgccggcctg catgtccttg agcgccacct
     5581 tgtggccgat cgggatgtcc tggcgggcct cgaggccgat cgtccggtct tcatccatga
     5641 tccagccggt catcgccgtg ccggccttca gcccttcgac gacgacgacc gccacggtgt
     5701 cttcggcgtc gtgcaatacg cagtgaatca ttgcgggtgt ctcctttttc cttgccgctt
     5761 cggcatccag ttcatggatg ctagccacgg caaagccctc gtcaagcagg tcatatgtat
     5821 gatacggatg aatgacgaaa ccaccggtgc cgcagtcgtt cggccgactt tcgcccgatg
     5881 tcgccggcat gccgctctat cgcgccgtcg aacgcgacct cctcggcgcg atcgagtcgt
     5941 cgcgcttcgg gcccggcgat gcgctgccga gcgaggccga cctcgcggcc tctttcggcg
     6001 tctcggtcgg gaccgtccgc aaggcggtcg a
//
LOCUS       DASRWS010000412         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1030208, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000412 DASRWS010000000
VERSION     DASRWS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030208"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFF72_07730"
     CDS             <1..206
                     /locus_tag="VFF72_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZV93092.1"
                     /translation="APHLVRRFAPSTHGFALVASAGAGGVLLLAADVLARSLIAPQEV
                     PVGVLTAILGGGYLIWLLHRGSR"
     gene            203..970
                     /locus_tag="VFF72_07735"
     CDS             203..970
                     /locus_tag="VFF72_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93093.1"
                     /translation="MTVIRVDKGSVTLGSTEALREVTLEVSSGWSAIVGPNGAGKSTL
                     LRAMAGLQALRRGQVSIDGRSLAEMAPDVRGGRLAWLSQQTDPTGELTVGETVALGRL
                     PSTGLFGAVTADDRRLVEQAMRDAGCLDARHRRLQELSGGERQRVYLARALAVGAPLL
                     LLDEPTTHLDPPYQVAFVRLLRDQVRAGVTVVSVLHDLSLALLADRLVIMDRGRIRSE
                     GPSDDPEVHRHLVDVFDGAVRIVRFDERWIVIPQVGA"
     gene            complement(1048..2082)
                     /locus_tag="VFF72_07740"
     CDS             complement(1048..2082)
                     /locus_tag="VFF72_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZV93094.1"
                     /translation="MSKQAELATIAGPVDVKDRPVRDDGSIQKTHAGLPVEEEADMVW
                     IPGGTFLMGSDHHYREEAPAHRVKVDGFRMDRHAVTNAQFQRFVEASGHVTLAERPAD
                     AVDYPDARLELLVPSSALFRKAVRRIDLGDHRAWWSLVPGTDWRHPRGPTSSLQGLWD
                     HPVVHVAFEDAAAYAEWAGKELPSEAEWEFAARGGLDGADYVWGDEFTPGGRQMANTW
                     QGEFPWENLLEDGYEWTAPVGSFPPNGYGLHNMAGNVWEWTSDWYQDHDRIESACCTL
                     DNPRGGDQWHSRDPGTIVRIPRRVIKGGSYLCAPNYCQRYRPAARMAQPVDTTTCHLG
                     FRCISRASTS"
     gene            complement(2202..2278)
                     /locus_tag="VFF72_07745"
     tRNA            complement(2202..2278)
                     /locus_tag="VFF72_07745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2242..2244),aa:Pro,seq:ggg)
BASE COUNT          354 a          834 c          812 g          414 t
ORIGIN      
        1 tggcaccgca cctggtgcga cggttcgcgc cctcgacgca tggcttcgcg ctggtggcaa
       61 gcgccggcgc ggggggtgtc ttgctgctgg ccgccgacgt tctcgcccgc tcgctgatcg
      121 cgccgcagga ggtgccggtc ggcgtgctca cggccatcct cggtggcggc tacctgatct
      181 ggctgcttca tcgcggctcc cgatgaccgt cattcgcgtc gacaagggaa gcgtcacgct
      241 cgggtccacc gaggcgctgc gcgaggtcac gctggaggtg tcctccggct ggtcggccat
      301 cgtcgggccg aacggcgccg gcaagtcgac cctgctgcgc gccatggccg gcctgcaggc
      361 gttgcgccgc ggccaggtct cgatcgatgg ccggagcctc gcagagatgg ctcccgacgt
      421 tcgcggcggc cgcctcgcct ggctgtcgca acaaaccgac ccgaccggcg agctgacggt
      481 gggcgagacg gtggcgttgg gccggctgcc gagcaccggc ctcttcggcg cggtgacggc
      541 cgacgatcgc cggctcgtcg agcaggcgat gcgcgacgcc ggatgcctgg acgcgcggca
      601 tcggcggctg caggagcttt ccgggggcga gcgccagcgc gtgtacctgg cacgagccct
      661 ggcggtcggc gcacccctgc tcttgctcga cgagcccacc acgcatctcg acccgccgta
      721 ccaggtggcg ttcgttcgcc tgctgcgcga ccaggtgcgc gccggcgtca cggtcgtcag
      781 cgtcctgcac gacctgtcgc tggcgctgct cgccgatcgc ctcgtcatca tggaccgcgg
      841 ccggatccgc tccgaaggcc cgtcggacga tcccgaagtg catcgacatc tcgtcgacgt
      901 tttcgacggt gccgttcgga tcgtccgctt cgacgagcgc tggatcgtca tcccgcaggt
      961 cggcgcctga cggcacgggc gactcgtcgc caacccggcc tggccttgct cgggcaccct
     1021 atcggatgcg tggtgtcgcc ccccctctta cgaggtcgaa gccctcgaga tgcagcggaa
     1081 cccgagatgg caggtcgtcg tgtcgaccgg ttgcgccatc cgggccgcgg gccggtatcg
     1141 ctggcaatag ttcggcgcac agaggtagga gccgcccttg atcacccggc gaggaatgcg
     1201 gacgatggtt ccggggtcgc ggctatgcca ctgatcaccg ccgcgcgggt tgtcgagcgt
     1261 gcaacaggca ctctcgatcc tgtcgtggtc ctggtaccag tcgctggtcc attcccagac
     1321 gtttcccgcc atgttgtgca ggccgtagcc gttgggcgga aatgagccga caggcgccgt
     1381 ccattcgtag ccgtcctcga gcaggttctc ccagggaaac tcgccctgcc aggtattggc
     1441 catctgccgg ccgccaggag tgaactcgtc gccccacaca tagtccgcgc catcgaggcc
     1501 accgcgcgcc gcgaattccc attcggcttc gctcggcaac tccttgccgg cccactcggc
     1561 gtaggccgcg gcgtcttcga aggcgacatg cactaccgga tggtcccaca agccctgcag
     1621 cgaactcgtc gggcctcgcg ggtggcgcca atcggtgccg ggaaccaggc tccaccaggc
     1681 gcggtggtcg ccgagatcga tacgccgcac ggccttgcga aagagcgctg aggaaggcac
     1741 cagcaactcg agcctggcat ccggatagtc gaccgcgtcc gccggtcgct ccgcgagtgt
     1801 gacgtgccca gacgcctcga cgaaacgctg gaactgggcg ttggtcaccg cgtgccggtc
     1861 catgcggaat ccatccacct tgacgcgatg tgcaggagct tcctcgcgat agtggtggtc
     1921 ggaccccatc aggaaggtgc cgcccgggat ccagaccatg tccgcctctt cttcgactgg
     1981 caggcctgca tgggtcttct ggatgctgcc gtcgtctcga accgggcggt ccttcacatc
     2041 gacgggcccg gcgatcgttg ccagctcagc ttgtttcgac acggcagttc cggacaggac
     2101 ttggtccatt cgaagtagtc gaatcacgcc ggccgcgaat tgccggctca cgaacgacaa
     2161 aacgggctag ctgctcatcg ctgctagccc gttgacttca ttggtcggga cggcgggatt
     2221 cgaactcgcg accccttgca ccccatgcaa gtgcgctacc aggctgcgct acgccccgaa
     2281 ggtcgcgatt ataggtggcg gccgcgacgc ttcacggagc gagcaggcca cggatcgaga
     2341 tcagttcctg gcggacctgg gccgccgaga gcgtgaagac aggcaggccg acatcggtgc
     2401 tctgccgatc ggtc
//
LOCUS       DASRWS010000413         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2189627, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000413 DASRWS010000000
VERSION     DASRWS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189627"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VFF72_07750"
     CDS             complement(<1..1258)
                     /locus_tag="VFF72_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HZV93095.1"
                     /translation="MNGSAAGDAVFAPRSDGQPLEAGGEPVAGVEHAQAPGRTAADKV
                     RCDACPVLCQIGRGKLGACDRYGNVDGVLTRTDTLQIVARPGVDLVAWQSAQGGWDGS
                     LVAADAPVFVSGNGSGTTYPDYKPAPFIVSSRHEGVDLVTVVTEGIFSYCSFKVKIDT
                     DRYLGPEQATVRHRGEAVGHVGTIEYGSQFLSLGGVHHLTGGSKKEGRIACEMMLALG
                     NREPVELQIEGGASVVVGADRAPVVDGIEERRMRVGCGSATIGIFAPQWHGHVDEVVV
                     VDDHITGVLSEHQAGKFLDLPPTGIRMRGRRSTPGRYFQVAQPGAGWGGTDISDPLAI
                     IEGWEGDRGARPGLRLLMVSTTGEDSAWFVLDEQLVPRRADMPAEVRSVVERIGENCE
                     PSLCSVLYVAGAGGSLRAGVTDNPVRL"
     gene            complement(1255..1434)
                     /locus_tag="VFF72_07755"
     CDS             complement(1255..1434)
                     /locus_tag="VFF72_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93096.1"
                     /translation="MSTTIVRILTDAVEGLLRVLVDATQQFADGLAELCLRVAHGVAE
                     GVVRVVVEAMRGLLA"
     gene            complement(1431..2060)
                     /locus_tag="VFF72_07760"
     CDS             complement(1431..2060)
                     /locus_tag="VFF72_07760"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZV93097.1"
                     /translation="MRLELDGPCLIGVRAGVRRVADLREWPADANGDVAVASGSFNPP
                     TIPLDADGQGVPYATYGFAAQLAELEVDLELGTVRLVRIHAAHDVGRAINPTLVEGQI
                     HGGIAQGIGMALMEEYVSGRTDNLHDYLIPTVGDVPPITVHLIEDAEPLGPWGAKGVG
                     EPALVATAPAILNAIHDATGVRMREVPVTPSRLRAAIVAADAAAIRDGR"
BASE COUNT          309 a          790 c          760 g          297 t
ORIGIN      
        1 tgagccggac cgggttgtcg gtgacgccgg cgcgcaggct gccgccggcg ccggcgacat
       61 agagcaccga gcagagcgaa ggctcgcagt tctcgccgat gcgctcgacg acgctcctga
      121 cctcggccgg catgtcggcc cgccgcggca ccagctgctc gtcgagaacg aaccacgccg
      181 agtcttcgcc ggtggtcgag accatgagca ggcgcaggcc cggccgcgcg ccgcgatcgc
      241 cctcccagcc ctcgatgatg gcgagcggat cgctgatgtc ggtgccgccc cagccggcgc
      301 cgggctgggc gacctggaaa tatcggcccg gcgtcgaacg gcgcccccgc atccggatgc
      361 cggtgggcgg caggtcgagg aacttgcccg cctggtgctc cgagagcacg ccggtgatgt
      421 gatcgtcgac gacgacgacc tcgtcgacat ggccgtgcca ctgcggcgcg aagatgccga
      481 tcgtcgccga gccgcagccg acccgcatcc gccgctcttc gatgccgtcg acgaccggcg
      541 cccggtcggc gccgacgacg acgctcgcgc cgccttcgat ctgcagctcg accggctcgc
      601 ggttgccgag cgccagcatc atctcgcagg cgatgcggcc ttccttcttc gagcctccgg
      661 tcaggtggtg gacgccgccc aggctcagga actgcgagcc gtactcgatc gtgccgacgt
      721 ggccgaccgc ctcgccgcga tgccgcaccg tcgcctgctc ggggccgagg tagcgatcgg
      781 tatcgatctt caccttgaag ctgcaatagc tgaagatgcc ttcggtgacg acggtcacga
      841 ggtcgacgcc ctcgtgcctc gaagagacga tgaagggcgc cggcttgtag tcgggatagg
      901 tcgttcccga gccgttgccg gagacgaaga ccggcgcgtc ggcggcgacg agcgagccgt
      961 cccagccacc ctgcgccgat tgccaggcga ccaggtcgac gccggggcgg gcgacgatct
     1021 gcagcgtgtc ggtgcgcgtc aggacgccgt cgacattgcc gtagcgatcg caggcgccga
     1081 gcttgccgcg gccgatctgg cagaggaccg ggcaggcgtc gcagcgcacc ttgtcggcgg
     1141 ccgtccgccc aggggcctgc gcgtgttcga cgccggcgac cggctcgccg ccggcttcga
     1201 gcggctggcc gtcggagcgc ggcgcgaaga cggcgtcgcc cgccgcgctg ccgttcacgc
     1261 gagcagtccc cgcatcgctt cgacgacgac gcggacgacg ccctcggcca cgccatgggc
     1321 gacccgcagg cacagctccg cgagaccgtc ggcgaactgc tgcgtcgcat cgaccagaac
     1381 ccgcagcagc ccttcgacgg cgtcggtcag gatgcgcacg atcgtcgtgc tcatcggccg
     1441 tcccggatcg ctgcggcgtc ggcagccacg atggcggcgc gcagccggct cggcgtcacc
     1501 ggcacttcgc gcatgcgcac gccggtcgcg tcgtggatcg cgttgaggat cgccggcgcc
     1561 gtcgccacca gcgccggctc gccgacgccc ttggcgcccc acggcccgag cggctcggcg
     1621 tcctcgatca ggtgcaccgt gatcggcggc acgtcgccga ccgtcgggat caggtagtcg
     1681 tgcaggttgt cggtgcgccc gctgacgtat tcctccatca gcgccatgcc gatgccctgg
     1741 gcgatgccgc cgtggatctg gccctcgacg agcgtcgggt tgatggcgcg gccgacgtcg
     1801 tgggcggcgt ggatgcgaac gagccggacg gtgccgagct cgaggtcgac ctcgagctcg
     1861 gcgagttgcg cggcgaagcc gtaggtcgca tagggaacgc cctggccgtc ggcgtcgagc
     1921 gggatggtcg gcgggttgaa gctgccgctg gcgacggcga cgtcgccgtt ggcgtcggcc
     1981 ggccattcgc gcaggtcggc gacgcgacgc acgccagccc gcacgccgat caggcaaggg
     2041 ccgtcgagct cgagacgcac gccatcgcca tcgccaacgc catcgccatc gccatcgcca
     2101 tcctcaacgc caacgccaac gccaccctca acgccaacgc caacgccacc ctcaac
//
LOCUS       DASRWS010000414         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_752517, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000414 DASRWS010000000
VERSION     DASRWS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752517"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1257
                     /locus_tag="VFF72_07765"
     CDS             229..1257
                     /locus_tag="VFF72_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZV93098.1"
                     /translation="MRAKASSLPSVDDVASAAGVSTATVSRVLNTPDRVREALRKRVT
                     DAVLKLGYVPHAGARALKLQRSGTVGAIFPTVDNAIFAKAIEALQQRLAGAGLQLLIA
                     TSGYDIETETQQAMNLVSRGADAMALCGVSQSPRLLQFLRQRELPAVHAMTCPPPAGM
                     VCVGFDNARAIGQAVRYLLDLGHRRVAMLAGITQDNDRAAARVAGARQALAEAGLTLP
                     PSRLVERAYALADAREGFRVLMSANPAPTAVLCGNDVLAIGALLEAQAMGLAVPGAVS
                     IVGFDDLEMSRHIRPGLTTLHVPTESMWATVADRLIAALGQKPVPPATEVEVELLVRE
                     STGPAPRK"
     gene            complement(1311..1727)
                     /locus_tag="VFF72_07770"
     CDS             complement(1311..1727)
                     /locus_tag="VFF72_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93099.1"
                     /translation="MNRLALTGAFACLVSFAATAQPVYRCGSEYTHFACADARPLDVS
                     DPVSVERRAEALATADRERRLGDSMARERRAEAAAFRPPMAANIGPRVPPAAAKARPT
                     AAKKRRGKDKAKASDSVFDAETDFVARVPKTKKSAG"
     gene            complement(1724..2425)
                     /gene="pth"
                     /locus_tag="VFF72_07775"
     CDS             complement(1724..2425)
                     /gene="pth"
                     /locus_tag="VFF72_07775"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZV93100.1"
                     /translation="MIRLIAGLGNPGTEYAATRHNAGFWFVDALARELGVVLVPERSY
                     FGLAAKAQVDGRPLWLLEPTTFMNVSGKSVAALARFFKIAPDEILVAHDELDLLPGHA
                     KLKQGGSAAGHNGLKDIHAQLGSADYWRLRLGIGHPGVKADVIHYVLRKPSAEHRQEI
                     DRAIEQALSALAPLLAGDMERATMKVNARPPRPKPPAAPAAAAPSSTAGAIASESAPG
                     LVAPTDPPADKEPNR"
     gene            complement(2501..>2953)
                     /locus_tag="VFF72_07780"
     CDS             complement(2501..>2953)
                     /locus_tag="VFF72_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZV93101.1"
                     /translation="QVLLRDYQMHPFKPLVLHIDFQRVDATTKVTKKVPLHYVGEENS
                     PAVKTDACVVNHVVNELNIQCLASQLPEFISIDLSELAKGQSLHVSDIKLPNGVKVVT
                     HGKPNPVIVSASVQAAEAEEAPAAAPVVVAPAPAEKAKPKKDEKQNRK"
BASE COUNT          438 a          982 c         1035 g          498 t
ORIGIN      
        1 gtttcggtct ctgccgtctt gctggccttc ttcaggtatt ccactggcat gagacgcgtc
       61 ccttcaaagg ctgccggcga gcgttccgat tcttgtaatc gattgcagaa tgcggatcat
      121 catcggcact cgcgctttgc gtcaagtcct gatctcccgg gtactttccc tgagcattcg
      181 gaaaacgcag cggcctcgga ccgcaaagca atcgattaca gtcaatcgat gcgagcgaag
      241 gcctccagcc tgccgagcgt cgacgacgtc gcctcggctg cgggtgtgtc cacggccacc
      301 gtctcgcgcg tgctcaatac gcccgatcgg gtgcgcgagg cattgcgcaa gcgcgtcacc
      361 gacgcggtgc tcaagctcgg atacgtgccg cacgccggcg cccgggcgct caagctgcag
      421 cgcagcggca cggtgggtgc gatttttccg acggtcgaca acgcgatctt cgccaaggcc
      481 atcgaggcgc tgcagcaacg actggcaggc gctggcctgc agctgctgat cgccaccagc
      541 ggctacgaca tcgagacaga aacgcaacag gcgatgaacc tggtctcgcg cggagccgac
      601 gcgatggccc tttgcggcgt cagccagagc ccgcggctgc tgcaattcct gcgtcagcgc
      661 gagttgcctg ccgtgcacgc gatgacgtgc cctccgccgg caggcatggt ttgcgtcggc
      721 ttcgacaacg cccgcgcgat cggccaggcc gtgcgctatc tgctcgacct cgggcatcgg
      781 cgcgtcgcca tgctggccgg catcacgcag gataacgacc gggcggcggc gcgcgtcgct
      841 ggtgcgcgac aggcgctcgc cgaggccggg ctgacgctgc cgccgtcgcg cctcgtcgag
      901 cgcgcctatg cactggccga cgcgcgtgag ggctttcgtg tcctgatgtc ggcgaacccg
      961 gcgccaaccg cggttctgtg cggcaacgac gtgctggcca tcggtgcctt gctcgaggcg
     1021 caggcgatgg gcctggcggt gccgggcgcg gtctcgatcg tcggcttcga cgacctcgag
     1081 atgtcacgcc atatccgtcc cggtctgacg acgctgcacg ttcccaccga atcgatgtgg
     1141 gcgacggttg ccgatcgcct gatcgccgcg ctcgggcaaa agcccgtacc gcccgcgacg
     1201 gaggtcgagg tggagctgct ggtgcgcgaa tcgaccgggc cggcaccacg caagtgaggc
     1261 ggtcgccagc gcggccggca ccacaagtga ggcggccgcc agcgcgccgc tcagccggcg
     1321 ctcttcttcg ttttcggcac ccgggcgacg aagtccgtct cggcgtcgaa gacggagtcc
     1381 gacgccttcg ccttgtcctt gccgcgcctc ttcttcgccg ccgtcggcct ggccttggca
     1441 gcggcaggcg gtacgcgcgg gccgatgttg gcggccatcg gcggacgaaa ggcggccgcc
     1501 tccgcgcgcc gctcgcgcgc catcgaatcg ccgaggcgac gctcgcgatc cgccgtcgcg
     1561 agcgcctcgg cgcgccgctc gacgctgacg gggtcggaga cgtcgagcgg gcgcgcgtcg
     1621 gcgcatgcga aatgggtgta ttcgctgccg cagcgataga cgggttgcgc cgtggcggcg
     1681 aacgacacga ggcacgcgaa ggcgccggtg agggcgaggc ggttcatcga tttggctcct
     1741 tgtcggccgg cggatctgtc ggcgccacca ggcctggcgc ggactcggaa gcaattgcac
     1801 cggccgtcga cgaaggcgcg gcggctgcgg gagccgcagg gggcttgggg cgcggcggcc
     1861 gcgcgttgac cttcatggtc gcccgttcca tgtcgccggc aagcagcgga gcgagcgccg
     1921 aaagcgcctg ctcgatggcc cggtcgatct cctggcgatg ctcggcggag ggctttctca
     1981 gcacgtaatg gatgacgtcg gccttgacgc cggggtggcc gatgcccagg cgcagccgcc
     2041 agtagtcggc gctgccgagc tgcgcgtgga tgtccttcag cccgttgtgg ccggcggccg
     2101 agccgccctg cttcagcttc gcatgccccg gcaggagatc gagctcgtcg tgggcgacga
     2161 ggatctcgtc cggcgcgatc ttgaagaatc gcgccagcgc cgccaccgac ttgcccgaaa
     2221 cgttcatgaa cgtcgtcggc tcgaggagcc agagcgggcg gccgtcgacc tgtgccttgg
     2281 cggccaggcc aaagtacgag cgttcgggaa cgaggaccac cccgagctcg cgcgccagcg
     2341 catcgacgaa ccagaagccg gcgttatggc gcgtcgcagc gtattcggtg cccgggttgc
     2401 ccaggcctgc gatcaatcgg atcatgggcg gaattatcga ggccgaccgg caagaaaaag
     2461 ccccgccgaa gcggggccgc gagacgcccg tgggggcgca tcacttgcgg ttctgcttct
     2521 cgtccttctt gggcttggcc ttttcggcag gtgcgggggc gacgaccacc ggtgccgcgg
     2581 cgggcgcttc ctcggcttcg gccgcctgca cgctggccga gacgatcacc gggttgggct
     2641 tgccgtgggt gacgaccttg acgccgttcg gaagcttgat gtcgctcacg tgcagcgact
     2701 ggcccttggc gagctcgctg aggtcgatcg agatgaattc aggcaactgc gaagccagac
     2761 actggatgtt cagctcgttg acaacgtgat tgacgacgca ggcatcggtc ttcaccgccg
     2821 gcgagttctc ctcgccgacg tagtgcagcg gcaccttctt cgtgaccttc gtcgtcgcgt
     2881 cgacgcgctg gaagtcgatg tgcaggacca gcggcttgaa gggatgcatc tggtagtcgc
     2941 gcagcagcac ctg
//
LOCUS       DASRWS010000415         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3904837, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000415 DASRWS010000000
VERSION     DASRWS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3904837"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1050
                     /locus_tag="VFF72_07785"
     CDS             160..1050
                     /locus_tag="VFF72_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZV93102.1"
                     /translation="MARYDDLAGFAAVVRARSFTRAAAQLGVTQSALSQTVRSLERRL
                     DLKLLNRTTRSVSPTEAGERLFQTVGPRLEDIEAELAVLGELRGKPAGTVRITASEHS
                     VRSLIWPRLEPWLPQYPDIKVEISGDNRFTDIVAERFDIGVRLGGDVAKDMIAVRIAP
                     DLRMVVVGSPGYFSRHPRPIAPQDLTAHDCIGLRLASHGGLLKWEFKRRRRSVNAHVS
                     GRLVFNSSELILAAALSGHGLAWLPLDMTQEHVQGGRLISALDDWAISFTGYHAYYAS
                     RRSSPALMLVVDALRPPVLP"
     gene            complement(1323..1994)
                     /locus_tag="VFF72_07790"
     CDS             complement(1323..1994)
                     /locus_tag="VFF72_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93103.1"
                     /translation="MRVVRFGSSNLRRSLLSVALGLGVAALSTQAFAACGGWPGAPAK
                     SSPSRWEVPTKATGGFMNIAYRPGDGRFVPVDLRESFEGIVGTWRFTWTSDGNAYPHP
                     VPYGAQVDFGTHQWHSDRTEMFTSGSRPPSSGDICMGVWKQTGRFTYKMRHIALAWLT
                     ADTIPPSNPPTYQGPAIMTETVTLNRAGDRYEGTFTIDQYAPDETTLIEHVSGKVEAT
                     RFTIE"
     gene            complement(2101..3078)
                     /locus_tag="VFF72_07795"
     CDS             complement(2101..3078)
                     /locus_tag="VFF72_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZV93104.1"
                     /translation="MNANVNEFVLSLYRAAKRLPHPSLRRWIFAEMQQLVHFDSALRF
                     RWSECGAGAPLQACHAWGQPDRQLDEYLALELWRDDIVHSVRPPNDRPWSIRASCAEC
                     GPERLRLFRKHYGQAHALVCWVPTDVAMVFDGVALHRAEAAEPFSDDDAARIEQLMPH
                     VADAWRESWLRELARSASSEPGFEGFSIAVLMPDLTMSEAQGDFAELLRLEWPRWRGP
                     LLPADLRTHLEATSPKPWSGKSITVYLRQQPDGTLLLLARRAHRLDAIAPRKREVALL
                     FSRGASQTQIARQLQLSPSTVNNYIGLVYEALALRDKTDLSRLVVRLEP"
BASE COUNT          539 a         1045 c         1039 g          574 t
ORIGIN      
        1 agccgaggcc gagagccgaa acggtgaggc ctccgcggcc gagttgtcgt gtcttcatgt
       61 gaggtgtcct tgtcgctatc gatgggaaga actttaggca gcctggcgtc gaatgactag
      121 actgctggat cgaaatgcac ctagaaggca ggctaatcaa tggctcggta cgacgacctt
      181 gcgggcttcg cggcggtggt tcgcgcaaga agcttcacac gcgccgccgc gcagctcggg
      241 gtgacgcagt ctgcgctcag ccagaccgtc cgctcgctgg agcggcgact ggatctgaag
      301 ctgctcaacc ggaccacgcg cagcgtctct cccaccgagg cgggcgagcg cctgttccag
      361 accgtggggc cgcgcctcga agacatcgag gcggagcttg ccgttctggg cgaactgcgc
      421 ggcaagccgg cgggcactgt ccgcatcacg gcgagcgagc actccgtgcg cagcttgatc
      481 tggccgaggc tcgagccctg gctgccacag taccccgaca tcaaggtgga gatcagcggc
      541 gataaccgct tcaccgacat cgtcgccgaa cgcttcgaca tcggtgtgcg tctgggtggc
      601 gacgttgcca aggacatgat cgccgtgcgc attgcccctg acttgcgcat ggtcgtggtt
      661 ggcagtccgg gctatttctc ccggcatcct cgaccgatcg ctccgcaaga tcttacggcg
      721 catgactgca tcggcctgcg cctggcctcg cacggaggtc tcctgaagtg ggagttcaaa
      781 cgccgacgca gaagcgtcaa cgcccacgtc tcaggtcggc tggtcttcaa ttcaagcgag
      841 ctgattcttg ccgcggcgtt atcgggacac ggtctcgcct ggctgccact ggacatgact
      901 caggaacacg ttcagggggg tcgattgatc tcggcgctcg acgattgggc catctccttc
      961 accgggtacc acgcgtacta cgccagccga cggagttcgc ctgcgttgat gttggtcgtc
     1021 gacgcgttac ggccgcccgt gcttccgtga gttcgtctga cgcaattcgt cgaggagtcg
     1081 ctcgatggcg tagcgtgtca ggagaaggtc catcgcccgg tgccgcgtgc ttgccaggtg
     1141 gagcagtctg cgcgcaccaa cgcgccgatg gctcaaaact ccttagccgc ctgcaatcag
     1201 tgccatgagg tatggctccc acccacggtt ccgatgactg accgcggcca ggtcgaaagc
     1261 cggcccgaag ccgccggcct tcgatatcgt caacgcgacg tcgatgaatt ggccaacccg
     1321 tgctattcga tcgtgaaccg cgtcgcctcg accttgccgc tgacatgctc gatcaacgtc
     1381 gtttcgtccg gggcgtactg gtcgatcgtg aacgtgcctt cgtagcgatc gcccgcgcgg
     1441 ttcaaggtca ccgtctcggt catgatggct ggcccctggt aggtgggagg gttcgaaggc
     1501 ggaatggtgt cggccgtgag ccatgccagg gcgatgtgcc tcatcttgta ggtgaagcga
     1561 ccggtctgct tccacacgcc catgcagatg tcgcccgagc tgggcggcct gcttcccgac
     1621 gtgaacatct ccgtcctgtc gctgtgccac tgatgcgttc cgaagtcgac ctgggcgccg
     1681 tagggcaccg gatgcggata cgcgtttccg tccgaggtcc aggtgaaccg ccatgttccg
     1741 acgatgcctt cgaacgactc tcgaaggtcg acgggcacga atcgtccgtc gcccggccgg
     1801 taggcgatgt tcatgaagcc gcccgttgcc ttggtgggca cctcccagcg cgagggcgag
     1861 ctcttggccg gcgcgccagg ccagcccccg cacgcggcaa aggcctgggt ggacagggcc
     1921 gcgacgccga gcccgagagc gaccgagagc agactcctgc gcagattcga cgatccgaag
     1981 cgaacgacac gcatgacttt ctccgtggag tggaatgggt cattcatcat cgaacgcgga
     2041 tttcgcgtct tcaagggggc ggttgcccct cccctgccgc ccggaactcg gcgcaccggg
     2101 tcaaggctcg aggcgaacga ccagcctcga cagatcggtc ttgtccctca gcgcgagcgc
     2161 ttcgtagacc aggccgatgt agttgttgac cgtcgatggc gagagttgca gctgccttgc
     2221 gatctgcgtc tgcgatgcgc cgcgcgaaaa cagcagcgcc acctcccgct tgcgcggcgc
     2281 gatggcatcg agtcgatggg cgcgccgggc gagcagcagc agcgtgccgt cgggctgctg
     2341 ccgaaggtac acggtgatcg atttcccgct ccagggcttg ggcgaggtcg cttcgagatg
     2401 agtcctcaag tcggctggca gcagcggccc gcgccagcgc ggccattcga gtcgcaaaag
     2461 ctcggcgaaa tcgccttgcg cttccgacat cgtcaggtcc ggcatgagca cggcgatgct
     2521 gaagccctcg aagcccggct ccgagctcgc ggagcgcgcc agctcgcgaa gccagctctc
     2581 gcgccatgcg tcggccacgt gcggcatcag ttgctcgatc cgcgcagcat cgtcgtcgct
     2641 gaacggctcc gccgcttcgg cccggtgtag cgccacgccg tcgaacacca tcgccacgtc
     2701 cgtcggcacc cagcacacca gggcgtgcgc ttgtccgtag tgcttgcgaa agagccgcag
     2761 ccgctccggg ccgcactcgg cgcatgaagc acggatcgac cacggccggt cgttcggcgg
     2821 cctgaccgaa tgaacgatgt cgtctctcca cagctcgagc gcgaggtatt cgtcgagctg
     2881 ccgatcgggc tgcccccatg cgtggcacgc ctgcaatggc gcccctgccc cacactccga
     2941 ccagcgaaac cgcaacgccg aatcgaagtg aaccagctgc tgcatctcgg cgaagatcca
     3001 gcgccgaagg ctcggatgcg gcagccgctt ggctgcgcgg tagaggctca gcacgaactc
     3061 gttgacgttg gcgttcatga cagcgaaggc ggccagtcca tggcgtcaca tgtgcttgaa
     3121 ctgatgcgcc tgcgccgcac tcacccgcag cttttcgggg cgtgatcgga ggctcagctc
     3181 gaggtggccg ccgatcg
//
LOCUS       DASRWS010000416         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3356517, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000416 DASRWS010000000
VERSION     DASRWS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356517"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFF72_07800"
     CDS             <1..214
                     /locus_tag="VFF72_07800"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867836.1"
                     /note="class II aldolase; catalyzes the reversible aldol
                     condensation of dihydroxyacetonephosphate and
                     glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis
                     and gluconeogenesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase"
                     /protein_id="HZV93105.1"
                     /translation="TAAIRKYLAENPEKFDPRDYLKPAREAARKICRERYLQFGCEGQ
                     AAKIAAQPLAQVARRYAQGELAQTVV"
     gene            385..1299
                     /locus_tag="VFF72_07805"
     CDS             385..1299
                     /locus_tag="VFF72_07805"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV93106.1"
                     /translation="MSTRIGFVGLGTMGTVMCQRLMGAGYALTVWNRSEAAGAALVKA
                     GATWAGSPKEVAAATDVVFSMVTDAAASEAVICGPGGLLEGTHPGLVIIDSASIMPEA
                     SQAIAQRVTAAGAAMLDAPVSGGPKVAAEGKLGIMVGGPEAAFKTCEPILKQLGSMVL
                     YTGANGNGTTLKLIANLVMGVAIQASAEALVLAAKVGIDPQRVIDITSLPGTGPQTGA
                     MATRGPRMLTHHFMPAHFSANNMDKDLTGALRLAEKHGVSLPATSAAREILRAVKSQG
                     NGQIDSSAVVTVLEAMAGTSVPGAHKAK"
     gene            1348..1725
                     /locus_tag="VFF72_07810"
     CDS             1348..1725
                     /locus_tag="VFF72_07810"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV93107.1"
                     /translation="MKKALQPKQWTPRGPYSLGTQLGNMAFLAGQIAQDNDGKTIGAG
                     DVKAQTAVVIDKIEQILAEAGMGLDDVMSTTVYLQSLVDYAAMNEVYKTRFKGVMPAR
                     ATVRADLAGDGLLVEISAIAARA"
     gene            complement(1762..2163)
                     /locus_tag="VFF72_07815"
     CDS             complement(1762..2163)
                     /locus_tag="VFF72_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV93108.1"
                     /translation="MAKIRHIALSVQDPEKTARFYEDALGLVRVGSTESALADGVYLS
                     DGYINIALLKYKSDAMAGVAGGKDFVGTHHFGFKVDDAEAARRQIEAHGGKFFMDLPA
                     LKDTLYYEEKYRDPEGVIFDISGKGWVTEAS"
     gene            complement(2207..2974)
                     /locus_tag="VFF72_07820"
     CDS             complement(2207..2974)
                     /locus_tag="VFF72_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV93109.1"
                     /translation="MWVEVRGKKLAVEKQGSGDAVVLVHGLGSTANVWEPQVRALADR
                     FTMLRYDLEGAGRSAYEGPLSIDGWVQDLKALLDVERIDKARLVGHSLGTLILQHFAV
                     AYPARVDRLVFIGVNRAPPEARRQAVRERVAKVRAEGLDAIVETVVKGGVSPHTLAHK
                     PEVVGFVRELLTRQPTEGYARCCEAMASSVAADVAAVKAPVLLIAGRDDGVSPPANSE
                     GMAKDLPNAQLRILEQCGHWHPIEQPAAVSQALREFL"
     gene            complement(3178..4152)
                     /locus_tag="VFF72_07825"
     CDS             complement(3178..4152)
                     /locus_tag="VFF72_07825"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93110.1"
                     /translation="MRNARRVVLVSAIGAAVAAIASTGHAASGSPANVEKVNFRLNWV
                     VAGNHAPFYLAQQKGLWSRCGLDVTMTPGTGSGATAQLVANGSQEFGLTDAVSITAAR
                     AHGLPVKSIGALYQTNPSSIVSKKSAGIVKLQDVAGKTWGAVPSGSPYLLLKALFKKY
                     NITTVKEADVPSPGIAQLKTGQVDFITFFGNEAANVDPDYKKDLNIVWFKDYGQDIYG
                     LAIATGDDYLKSHPQQVACFRQGVIEGFKEAQANPEAALQALYAGAPLTKSKPEVQAA
                     LLQGALDFTGTDLLAQSTAKWQATQELLFEAGITPKKVDPSSLFWQPR"
     gene            complement(4181..>4463)
                     /locus_tag="VFF72_07830"
     CDS             complement(4181..>4463)
                     /locus_tag="VFF72_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZV93111.1"
                     /translation="KVRLPHALPHIFSGLKIGITLAVVGAVVGEWVGANSGLGYLLLH
                     ANSMLDTTLLYASLVLLIVMGVVLFVLMEIIERVLMPWQEPETPAPVTA"
BASE COUNT          716 a         1496 c         1539 g          712 t
ORIGIN      
        1 gacggcggcg atccgcaagt acctggccga gaaccccgag aagttcgatc cgcgcgacta
       61 cctcaagccc gcgcgcgaag cggcccgcaa gatctgccgc gagcgctatc tgcagttcgg
      121 ctgcgaaggc caggcggcga agatcgccgc ccagccgctg gcccaggtgg cgcggcgtta
      181 tgcgcagggg gaattggcgc agacggtcgt ctgacgacgc gcctgcacgc ggccctcgcc
      241 gggtcgagga aactcgggcc ggcgccgcat gtcctcgagg agcctgggct aaagtcgtcg
      301 caccgagcag ccgcgacgcc gagcttcggc accgcgcccg tacgctgcgt cggcgctggc
      361 agttttcaaa cggaaggaat gaggatgagc acgcgaatcg gcttcgtcgg cctgggcacg
      421 atgggcaccg tcatgtgcca gcgcctgatg ggcgcaggtt atgcgctcac ggtttggaac
      481 cgctccgagg ccgccggcgc cgccctggtg aaggccggcg cgacctgggc cggctcgccc
      541 aaggaagtgg cggccgcgac cgacgtggtc ttctcgatgg tcaccgacgc ggcggcatcg
      601 gaggccgtga tctgcggccc cggcggcctg ctcgaaggca cgcacccggg cctcgtcatc
      661 atcgattcgg cgagcatcat gcccgaggcc tcgcaagcca tcgcccagcg ggtcacggcg
      721 gccggcgcgg cgatgctcga cgcgccggtg tcgggcggcc ccaaggtcgc cgccgaaggg
      781 aagctgggca tcatggtcgg cggccccgag gcggcgttca agacctgcga gccgatcctc
      841 aagcagctcg gcagcatggt cctctacacc ggcgccaacg gcaacggcac gaccttgaag
      901 ctgatcgcca acctggtcat gggcgtggcg atccaggcct cggccgaggc actggtgctg
      961 gcggccaagg tcggcatcga cccgcagagg gtgatcgaca tcacctcgct gcccggcacc
     1021 ggaccgcaga ccggcgcgat ggccacgcgc ggcccgcgca tgctcacgca ccacttcatg
     1081 ccggcgcact tctcggccaa caacatggac aaggacctga ccggcgcctt gcggctggcc
     1141 gagaagcacg gcgtgtcgct gccggcgacc agcgcggcac gcgaaatcct gcgcgccgtc
     1201 aagtcgcagg gcaatggcca gatcgactcg agcgccgtcg tcaccgtgct cgaggccatg
     1261 gccggcacct cggtgcccgg cgcccacaag gcgaaatagg cgtccgccgg acgcccgccg
     1321 cattcacaac acctggagca gaaagacatg aagaaggccc tgcaaccgaa gcaatggacg
     1381 ccgcgcggcc cctactcgct gggcacccaa ctgggcaaca tggcgttcct cgccgggcag
     1441 atcgcccagg acaacgacgg caagacgatc ggcgccggcg acgtgaaggc acagaccgcg
     1501 gtcgtcatcg acaagatcga gcagatcctc gccgaagcag gcatggggct ggacgacgtg
     1561 atgtcgacga cggtctacct gcagtcgctt gtcgactacg ccgccatgaa cgaggtctac
     1621 aagacgcgct tcaagggcgt catgccggcg cgcgccacgg tccgcgccga cctcgccggc
     1681 gacggcctgc tggtcgagat ctcggcgatc gccgcgcggg cctgacgccg gcgcgcggcc
     1741 gcccgggcga cgctgcccgc gtcagctcgc ctcggtgacc cagcccttgc ccgagatgtc
     1801 gaagatgacg ccttcggggt cgcggtactt ctcttcgtag tacagcgtgt ccttcagcgc
     1861 cggcaggtcc atgaagaact tgccgccgtg cgcctcgatc tgcctgcgtg cggcctcggc
     1921 gtcgtcgacc ttgaagccga aatggtgggt gccgacgaaa tccttgccgc cggccacgcc
     1981 ggccatggcg tcgctcttgt acttgagcag cgcgatgttg atgtagccgt cggacaggta
     2041 gacgccgtcg gccagcgccg attcggtgct gcccacgcgt accaggccga gcgcgtcttc
     2101 gtagaacctg gccgtcttct cgggatcctg gaccgacagc gcgatgtggc ggatcttcgc
     2161 catggcttct tgctccttga atgggtgagg gcctgcgcaa caggtcctag aggaattcgc
     2221 gcagggcctg gctgacggcg gccggctgct cgatcgggtg ccagtggccg cactgttcga
     2281 ggatgcgcag ctgcgcgttc ggcaggtcct tggccatgcc ttcgctgttg gcgggcgggc
     2341 tgacgccgtc gtcgcggccg gcgatgagca ggaccggtgc cttgacggct gcgacgtcgg
     2401 cggcgacgga agacgccatg gcctcgcagc agcgcgcgta gccttcggtc ggctggcggg
     2461 tgagaagctc gcgaacgaag cccaccacct cgggcttgtg cgccagcgtg tgcggcgaga
     2521 cgccgccctt gaccaccgtc tcgacgatcg cgtcgaggcc ttcggcgcgg accttggcca
     2581 cgcgttcgcg aaccgcctgg cgacgcgcct cgggcggcgc ccggttcacg ccgatgaaga
     2641 cgagcctgtc gacgcgggcc gggtaggcca cggcgaagtg ctgcaggatg agggtaccca
     2701 gcgaatgacc gaccaggcgc gccttgtcga tgcgctcgac gtcgagcaag gccttcagat
     2761 cctggaccca gccgtcgatc gacagcggcc cttcgtacgc ggagcggccg gcgccctcga
     2821 ggtcatagcg cagcatcgtg aagcgatcgg cgagcgcgcg cacctgcggt tcccagacgt
     2881 tggccgtgct gcccagccca tggacgagca ccacggcatc gccgctgccc tgcttctcga
     2941 cagcgagctt cttgcctctg acttctaccc acatcgtttc gtgctccgga aatcggaatt
     3001 ggccaacatc gccgggcctg cggcacgcgg gccggcgttt gcgacagtat gcgtcggtcg
     3061 cgatagccgc acacccgcca tgtcgctgcg cggcggccgt cgcccgcagc ccgcgcggcg
     3121 ctcgtcgcgt caaggctcgg gcgcgtcgac agcccgacgc gggcggcgct gcgtcgccta
     3181 cctgggctgc cagaacaagc tgctcgggtc gacctttttc ggcgtgatgc cggcttcgaa
     3241 cagcagctcc tgcgtcgcct gccacttggc cgtgctctgg gccaggaggt cggtgcccgt
     3301 gaagtcgagc gcgccttgca gcaacgcggc ctggacctcg ggcttgctct tggtgagcgg
     3361 cgcgccggcg tagagggcct gcagcgccgc ctcggggttc gcctgggcct ccttgaagcc
     3421 ctcgatgacg ccttggcgga aacacgccac ctgctgcgga tggctcttga ggtagtcatc
     3481 gccggtcgcg atcgccaggc cgtagatgtc ctgcccgtag tccttgaacc agacgatgtt
     3541 cagatccttc ttgtaatccg gatcgacatt ggccgcctcg ttgccgaaga aggtgatgaa
     3601 gtcgacctgt ccggtcttga gctgggcgat tcccggggaa gggacgtcgg cttccttgac
     3661 ggtcgtgatg ttgtacttct tgaaaagcgc cttcagcagc aagtagggcg atccgctcgg
     3721 cacggcgccc caggttttgc cggcgacgtc ttgcagtttc acgatgcccg cgctcttttt
     3781 cgacacgatc gacgacgggt tggtctggta cagcgcgccg atcgatttca ccggcaagcc
     3841 gtgcgcgcgc gcggccgtga tggacacggc gtcggtgagc ccgaactctt gcgagccgtt
     3901 ggcaacgagc tgtgccgtgg cgcccgagcc ggtgcccggg gtcatggtga cgtcgaggcc
     3961 gcagcggctc cagaggccct tttgttgcgc caggtagaag ggcgcgtggt tgccggcgac
     4021 gacccagttg agacgaaagt tcaccttctc gacattggcc ggcgaaccgc tggcagcgtg
     4081 gccggtgctt gcgatggcgg cgactgccgc gccgattgct gagacgagga cgacacggcg
     4141 cgcgttacgc atgatctgtt tcctttcgac ggatggatgg tcaggcagtg actggagcgg
     4201 gcgtctccgg ctcctgccag ggcatgagga cccgctcgat gatctccatg agcacgaaca
     4261 gcacgacacc catgacgatc agcaagacga gcgaggcgta gagcaaggtg gtgtcgagca
     4321 tgctgttggc atgcagcagc aggtagccga gcccggagtt tgccccgacc cattcgccga
     4381 ccaccgcgcc gaccaccgcc agggtgatgc cgatcttaag tccggagaag atgtgcggca
     4441 aagcgtgcgg cagacggacc ttg
//
LOCUS       DASRWS010000417         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2742881, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000417 DASRWS010000000
VERSION     DASRWS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742881"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFF72_07835"
     CDS             <1..996
                     /locus_tag="VFF72_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZV93112.1"
                     /translation="VVATYLAHLKRRAEVAAGVPIGRLLLGRPVFFVDDDPARDAAAQ
                     AALRTAAQRAGFDDIAFQYEPIAAAFDYERSAEREEKVLVADIGGGTSDFSLVRVGPR
                     RMDRDDRKDDILANHGVHIAGTDFDRRIELANVLPSLGYGALGEAGPGSAAREVPSGV
                     YFDLATWHLVNTVYQPQRVAELRGMRSFYADPAQHRRLMSVVEQRLGHDLLARAEAAK
                     IAVAGGGTSTIDLTQVEGGLRSTLDEAHARRALSRDLDRIVEAAIETTRQAGLVADAV
                     DALYFTGGSTGLRLLTDRLCAAFPTARPVRGDRFASVANGLALAATRRFGGSQGR"
     gene            complement(1233..>2427)
                     /locus_tag="VFF72_07840"
     CDS             complement(1233..>2427)
                     /locus_tag="VFF72_07840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZV93113.1"
                     /translation="FGGDPILSLNDDFQKDPRPGKINLSIGIYFDDAGRIPVLDSVRR
                     AELQVVDRNAPKPYLPIEGAANFRSEVQKLLFGSGHEVLGSGRVVTIQSVGSSGGLKV
                     GADFIARWLPGSEVWVSDPSWDNHRSMFEGAGLVVHSYPYYDASTGSLAFEAMCETLR
                     RLPARSVVLLHACCHNPTGVDLSRAQWDALIPILAERELLPYLDLAYQGFGDGLAEDA
                     YAVRALVAARGKDGKPMPFFVANSFSKSMSLYGERCGALSVACADRDEAVNVLGQLKF
                     TIRRNYSSPPIHGGQVVAAVLGEPPLRQAWEHEVGAMRDRIHSMRKALHATLVQRMPG
                     HDFDYFLTQRGMFSYTGLSTGQVDRLRDEFAVYLVRSGRMCVAGLNTGNVEATGKAIA
                     RVLAG"
BASE COUNT          377 a          838 c          852 g          360 t
ORIGIN      
        1 gtcgtggcca cctatctggc gcacctgaag cgccgggccg aagtcgcggc cggcgttccg
       61 atcggacgac tgctgctcgg ccggccggtc ttcttcgtcg acgacgatcc ggctcgagac
      121 gctgccgcgc aagcggccct gcgaaccgcg gcgcagcggg caggtttcga tgacatcgcc
      181 ttccagtacg aacccatcgc ggccgcgttc gactacgagc gctcggccga gcgcgaggag
      241 aaggtcctgg ttgccgacat cggcggcggc acgtccgact tctcgctggt ccgggtcggg
      301 cctcgaagga tggatcgcga cgatcgcaag gacgacatcc tcgccaatca cggcgtccac
      361 attgccggga ccgacttcga tcgccggatc gagctggcga acgtgttgcc gtcactcggc
      421 tacggcgccc tcggcgaggc cggtccggga tcggcggcgc gcgaagtgcc gagcggcgtg
      481 tacttcgatc tcgcgacatg gcatctcgtc aacacggtct accagccgca gcgcgtcgcc
      541 gagttgcgcg gcatgcgctc gttctacgcc gaccccgcgc agcatcggcg actgatgagc
      601 gtcgtcgagc agcggctcgg ccacgatctg ctcgcccgcg ccgaggccgc gaagatcgcg
      661 gtcgccggcg gcggcacgag cacgatcgac ctgacacagg tcgaaggcgg cctgcgttcg
      721 acgctcgacg aggcacacgc ccgacgggcg ctctcgcgcg acctcgaccg gatcgtcgag
      781 gccgcgatcg agacgacgag gcaggccggg ctcgttgccg atgccgtcga cgccctctac
      841 ttcaccggcg gctcgaccgg cctgcgcctg ctgaccgatc gactgtgcgc tgcctttccc
      901 accgcgcggc cggttcgtgg cgatcgtttc gccagcgtcg cgaacggact ggcgttggca
      961 gcgacgcgtc gcttcggcgg ttcgcagggc cgctgaggat cggagaaatg ccgcagacga
     1021 gagcgaagcg cgagcctctc gatgcaacga ccggcgggaa tcggctggct gcgtcggccg
     1081 cacgtcgtcg gcagcccgcg acgccgtagc ccgcgacttc agcgcccgcg atgccagagt
     1141 ccgcgacgcc agagcccgcg acgccagagc ccgcgacgca gcagcctgca gccccgaggc
     1201 tcgctgccgt ggcccgcaaa ccccgcggat cgtcagcccg ccagcacgcg ggcgatggcc
     1261 tttcccgttg cttcgacgtt gccggtgttc agccccgcca cgcacatccg ccccgatcgc
     1321 acgaggtaga cggcgaactc gtcgcgcagg cgatcgacct ggccggtcga caggcccgtg
     1381 tagctgaaca tgccgcgttg cgtcaggaag tagtcgaagt cgtgtccggg catgcgctgc
     1441 accagcgtcg cgtgcagcgc cttgcgcatg gagtggatcc ggtcccgcat ggcgccgacc
     1501 tcgtgctccc aggcctggcg cagcggcggt tcgccgagca ccgcggcaac gacctggccg
     1561 ccatggatcg gggggctcga gtagttgcgc cggatcgtga acttgagctg gccgagcacg
     1621 ttgacggctt cgtcgcgatc ggcgcaggcg acgctgagcg cgccacaccg ctcgccgtac
     1681 aggctcatgc tcttcgaaaa cgagttggcg acgaagaacg gcatcggctt gccgtccttg
     1741 ccgcgggccg ccacgagggc gcggacggcg taggcgtcct cggcgaggcc gtcgccgaag
     1801 ccctggtagg ccaggtcgag gtaggggagc agctcgcgct cggccaggat cgggatcagc
     1861 gcgtcccatt gcgcccggga gaggtcgacg ccggtcgggt tgtggcagca ggcatgaagc
     1921 aggacgacgc ttcgcgccgg cagccggcgc agcgtctcgc acatcgcttc gaacgcgagc
     1981 gagccggtcg aagcgtcgta atacggatag ctgtgcacca caagaccggc gccttcgaac
     2041 atcgagcggt ggttgtccca gctcgggtcg ctgacccata cctcgctgcc gggcagccag
     2101 cgggcgatga aatcggcgcc gaccttgagg ccgccgctcg agccgaccga ctggatcgtc
     2161 acgacgcggc ccgagcccag cacctcgtga ccgctgccga agaggagctt ctggacctcg
     2221 ctgcggaagt tggccgcacc ttcgatcggc aggtacggct tgggggcgtt gcggtcgacg
     2281 acctgcaact cggcgcggcg caccgaatcg aggaccggga tgcggccggc gtcgtcgaag
     2341 tagatgccga tcgacaggtt gatcttgccc ggacgcggat ccttctggaa atcgtcgttc
     2401 aggctgagga tggggtcgcc gccgaac
//
LOCUS       DASRWS010000418         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2447849, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000418 DASRWS010000000
VERSION     DASRWS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447849"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1607
                     /locus_tag="VFF72_07845"
     CDS             69..1607
                     /locus_tag="VFF72_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HZV93114.1"
                     /translation="MSALLLNQTHTKPLAGPSDWSFELIEQYHDVIRATAESYKLDTY
                     PNQLEIITAEQMMDAYASVGMPVIYRHWSYGKEFIANERRYRRGQMGLAYEIVINSDP
                     CISYLMEENTTAMQALVIAHAAYGHNSFFKGNYLFRLWTDASSIIDYLVYARNYVAEC
                     EEKHGMAAVEDLLDSCHALQNHGVDRYHRPSRISLVEEQARRAERLAYAQAQVNDIWR
                     TLPRRADRADDAASSRRFPAEPQENLLYFIEKNAPLLEPWQREIVRIVRKVAQYFYPQ
                     RQTQVMNEGWATFWHHLLLNTLYDEGHLTDGVMIEWLKSHTNVVFQPPVGHPAYGGLN
                     PYALGFAMYTDIRRICEQPTEEDREWFPQLAGTPWLPALHHAMRNFKDESFIGQYLSP
                     KVIRDLRLFAILDDEHEEQLSVSAIHDNDGYRHVREALSRQYDLGSREPDIQVWSVDL
                     RGDRSLTLRHTRHHNRPLDEGAQEVLKHVARLWGFGVNLESVDAYGDVTTHWSVTPSE
                     LVRL"
     gene            complement(1674..3095)
                     /locus_tag="VFF72_07850"
     CDS             complement(1674..3095)
                     /locus_tag="VFF72_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZV93115.1"
                     /translation="MNAPLPAHLLPRLDPRPVPQAMLDALKTQFGERCSTAAAVREQH
                     GRDESPIDAPPPEAVVFCESTEDVAFVVRLADRFSVPVIPFGVGSSLEGHLLAVQGGV
                     SIDLSRMNKVLAVNAEDLTVTVQAGVTRTQVNEEIRHTGLFFPIDPGADASIGGMSAT
                     RASGTNAVRYGTMRENVLGLQVVTAQGEVIHTGTRARKSSAGYDLTRLMVGSEGTLGV
                     MTEITLRVYPLPEAMSAAICSFPTIDAAVRTTIQIIQMGVPIARCELLDRFAVQAVNR
                     HDKLSLPETPLLLMEFHGSEASVAEQAQIVQEIASEHGGEGFQWATTPEERKKLWTAR
                     HRAYFAGMQIKPGCRTVTTDTCVPISRLAESVVTASEEALAAGLRHYIVGHVGDGNFH
                     MAYLVDPAKPGERETAERLNEALVLRALAMDGTCSGEHGVGLHKMGFLVKEAGEDAIG
                     MMRTIKRALDPKNIMNPGKIFAV"
     gene            3182..3757
                     /locus_tag="VFF72_07855"
     CDS             3182..3757
                     /locus_tag="VFF72_07855"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZV93116.1"
                     /translation="MANRLSQIATRTGDDGTTGLGDGSRVAKDDRRIAALGDVDELNS
                     HLGVLLCEPLPDEVRKLLVVIQQELFNLGGELSIPGYELLKSDAVAALDAALARYNAD
                     LPRLKEFILPAGTRSAALAHVARTVARRAERSLVALAAQQVVRAEPRQYLNRLSDLLF
                     VLARVLNRANLDGLGGDDVYWQSARLARDAD"
     gene            complement(3899..>4823)
                     /locus_tag="VFF72_07860"
     CDS             complement(3899..>4823)
                     /locus_tag="VFF72_07860"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZV93117.1"
                     /translation="QRKDDRLLVIMGPCSIHDPLAAVEYARRLKVERDRYAGSLEIVM
                     RVYFEKPRTTVGWKGLINDPYLDETYRIDEGLRIGRQLLLEINRLGMPAGSEFLDVIS
                     PQYLGDLIAWGAIGARTTESQVHRELASGLSAPIGFKNGTDGNIRIAIDAIQAAARPH
                     HFLSVHKNGQVAIVETRGNVDCHVILRGGKSPNYDAASIDTACREIEAAGLPCTLMVD
                     CSHANSGKDPGRQSIVAADIAAQLKAGSRCIFGAMIESHLTGGAQKFSAGRDDPAALR
                     YGQSITDGCLGWDESLPVLEALSQAVLARRG"
BASE COUNT          800 a         1644 c         1580 g          799 t
ORIGIN      
        1 ggtcgaggca tgagccttgc cccatccgtg tcggctggcc tcgacgtgcc aagcccggag
       61 gtattcagat gagcgcgcta cttctcaacc agacgcacac caaacccctg gccggcccct
      121 cggactggtc attcgagttg atcgagcagt accacgatgt catccgcgcg acggcggaaa
      181 gctacaagct cgacacctat ccgaaccagc tcgagatcat caccgccgag cagatgatgg
      241 acgcgtatgc gtcggtcggc atgccggtga tctaccggca ctggagctac ggcaaggaat
      301 tcatcgccaa cgagcgtcga tatcgccgcg gtcagatggg cctggcctac gagatcgtca
      361 tcaattcgga tccgtgcatc tcctatctca tggaggagaa cacgacggcg atgcaggcgc
      421 ttgtcatcgc ccacgccgcc tacggtcaca acagcttctt caagggcaac tacctgttcc
      481 ggctgtggac cgacgcctcg tcgatcatcg actacctcgt ctacgcccgc aactacgtcg
      541 ccgaatgcga ggagaagcac ggcatggcgg cggtcgagga cttgctcgac agctgccacg
      601 cactgcaaaa ccacggcgtc gaccgctacc accggcccag ccgcatctcg ctcgtcgagg
      661 agcaggcgcg ccgggccgag cggctcgcct acgcgcaggc ccaggtcaac gacatctggc
      721 gcacgctgcc gcgtcgcgcc gaccgcgccg acgacgcggc gtccagcagg cgctttcccg
      781 ccgagccgca ggaaaacctt ctctatttca tagagaagaa cgcgccgctg ctcgagccgt
      841 ggcagcgcga gatcgtgcgc atcgtccgca aggtcgccca gtacttctat ccgcaacgac
      901 agacccaggt catgaacgaa ggctgggcca cgttctggca ccacctgctg ctcaatacgc
      961 tgtacgacga aggccacctg accgacggcg tcatgatcga gtggctgaag tcgcacacca
     1021 acgtggtctt ccagccgccg gtcggccatc cggcctacgg cggcctcaat ccgtatgcgc
     1081 tcggtttcgc gatgtacacc gacatccggc gcatctgcga acagccgacc gaggaagacc
     1141 gcgaatggtt tccgcaactc gccggtacac cgtggctgcc ggcgctgcac catgcgatgc
     1201 gcaacttcaa ggacgagagc ttcatcggcc agtatctgtc gccgaaggtg attcgcgacc
     1261 tgcgcctgtt cgccatcctc gacgacgagc acgaagagca gctgagcgtc tcggcgatcc
     1321 acgacaacga cggctaccgc catgtccgag aggcgctgtc gcgccagtac gacctcggct
     1381 cgagggagcc cgacatccag gtctggagcg tcgacctgcg cggcgatcga tcgctgacgc
     1441 tgcgccatac ccggcaccac aaccgaccgc tcgacgaggg cgcccaggaa gtgctcaagc
     1501 acgtcgcgcg cctctgggga ttcggcgtca acctcgagag cgtcgatgcc tacggtgacg
     1561 tgacgacgca ctggtcggtc acgccgtcgg agttggtccg cctgtagccc cgccggcgag
     1621 gcgacttcgt cgtcggcagc cgcacagggc ggctcggcgc gtcgccccgc gctctacacg
     1681 gcgaagatct tgcccgggtt catgatgttc ttcgggtcga gcgcgcgctt gatcgtgcgc
     1741 atcatgccga tcgcatcctc gccggcttcc ttcaccagga atcccatctt gtgcagcccg
     1801 acaccgtgct cgccgctgca ggtgccgtcc atggcgaggg cgcgcagcac cagcgcctcg
     1861 ttgaggcgtt cggcggtctc cctttccccc ggcttggcgg ggtcgacgag gtaggccatg
     1921 tggaaattgc cgtcgccgac atggccgacg atgtagtggc gaaggcccgc ggcgagggcc
     1981 tcctcggagg cggtgacgac cgactcggcc agccgcgaga tcggcacgca ggtgtcggtg
     2041 gtgacggtgc ggcagcccgg cttgatctgc atgccggcga aatacgcccg gtgacgcgcc
     2101 gtccacagct tctttcgttc ttcgggcgtc gtcgcccact gaaaaccctc gccgccgtgc
     2161 tcgctggcga tctcctggac gatctgcgcc tgctcggcga cgctcgcctc gctgccgtgg
     2221 aactccatca gcagcagcgg cgtctccggc aggctcagct tgtcgtgccg gttcaccgcc
     2281 tgcaccgcga agcggtcgag caactcgcag cgggcgatgg gcacgcccat ctggatgatc
     2341 tggatcgtcg ttctcaccgc ggcgtcgatc gtcggaaagc tgcagatcgc cgccgacatc
     2401 gcctcgggaa gcggatagac gcgcagggtg atctcggtca tgacgcccag cgtgccttcg
     2461 ctgccgacca tcaggcgcgt caggtcgtag cccgccgacg acttgcgggc acgcgtgccg
     2521 gtgtgaatga cctcgccttg cgccgtcacg acctgcaggc ccagaacgtt ctcgcgcatc
     2581 gtcccgtaac ggaccgcgtt ggtgccgctc gcccgcgtcg ccgacatgcc gccaatcgac
     2641 gcgtcggcgc ccgggtcgat gggaaagaag agaccggtgt ggcgaatctc ctcgttgacc
     2701 tgcgtgcgcg tcacgcctgc ctggacggtc accgtgagat cttcggcgtt cacggcgagg
     2761 accttgttca tgcgcgacag gtcgatcgag acgccgccct gcacggcgag cagatggccc
     2821 tcgagcgacg agccgacgcc gaacggaatc accggaaccg agaagcgatc ggccaggcgg
     2881 acgacaaagg cgacgtcttc cgtgctttcg cagaagacga ccgcctccgg cggcggcgca
     2941 tcgatcggcg attcgtcgcg accgtgctgt tcgcgcaccg ccgcggccgt cgagcagcgc
     3001 tcgccgaatt gcgtcttcag ggcatcgagc atcgcttgag gcaccggccg cggatcgagg
     3061 cggggcagga ggtgggcggg caacggtgcg ttcatggcga tctccccggc gacgagccgt
     3121 caggcattgt aggaagcagc gcattcctcg gcgcagtcgc gctgctagca tcgtgcgcac
     3181 catggcgaac cggctttccc agatcgcaac ccgaaccggc gacgacggca cgaccggcct
     3241 cggcgatggg tcgcgcgttg ccaaggacga ccggcgcatc gccgcgctcg gcgacgtcga
     3301 cgagctcaat tcgcacctcg gcgtcctgct gtgcgaaccg ttgcccgacg aggtgcgcaa
     3361 gctcctggtc gtcatccagc aggagctgtt caacctcggc ggcgaattgt cgattcccgg
     3421 atacgagctg ctgaagtccg acgccgtcgc cgccctcgac gccgcgctcg cgcgctacaa
     3481 cgccgatctg ccgcggctga aggagttcat cctgccggcg ggcacccgca gtgccgcgct
     3541 cgcgcacgtt gcgcgcaccg tggcacggcg agccgaacgt tcgctggtcg ccctcgctgc
     3601 gcagcaggtc gtgcgtgccg agccgcggca gtatctgaat cggctgagcg acctgctgtt
     3661 cgtgctggcg cgcgtcctca accgggccaa tctcgacggg ctgggcggcg acgacgtcta
     3721 ttggcagagt gccaggctgg cgcgcgacgc cgactgaaag ttcagctcga tgtgacgcca
     3781 tcggcccggc gggtcggaga tgacctgacg cggcgtcaga gcaacgtgcg gcggctttgc
     3841 gaggccagct tcttcgtgga ggtacgtggg catggcggaa gggactcgcc ccgccccttc
     3901 agccgcggcg agccagtacc gcctggctca gcgcctcgag cacgggcagc gattcgtccc
     3961 agcccaggca gccgtcggtg atgctctgac cgtaccgcaa ggccgccgga tcgtcgcggc
     4021 cggcgctgaa cttctgggcg ccgccggtca ggtggctttc gatcatcgcg ccgaagatgc
     4081 agcgactgcc ggctttcagc tgagccgcga tgtcggcggc gacgatcgac tggcggcccg
     4141 gatccttgcc gctgttggca tggctgcaat cgaccatcag cgtgcaaggc agcccggccg
     4201 cctcgatctc gcggcaagcg gtgtcgatcg acgcggcatc gtagttgggc gacttgccac
     4261 cgcgcaggat gacgtggcag tcgacatttc cgcgtgtctc gacgatcgcg acctggccgt
     4321 tcttgtgcac cgagaggaaa tgatgcgggc gcgccgccgc ctggatcgcg tcgatggcga
     4381 tccggatgtt gccgtcggtg ccgttcttga agccgatcgg cgccgacagg ccggaagcga
     4441 gctcgcggtg cacctggctc tcggtggtgc gggcgccgat cgccccccag gcgatcaggt
     4501 cgccgaggta ctgcggcgag atgacgtcga gaaattcgct gcccgccggc atgccgagcc
     4561 ggttgatctc gaggagcagc tggcggccga tgcgcaggcc ctcgtcgatc cggtacgtct
     4621 cgtccaggta gggatcgttg atcagtccct tccagcccac cgtcgtgcgg ggcttttcga
     4681 aatagacgcg catcacgatc tcgagcgacc cggcatagcg gtcgcgctcg actttcaggc
     4741 gccgcgcgta ctcgaccgct gcaagcggat cgtggatcga gcaggggccc atgatcacga
     4801 ggaggcgatc gtccttgcgc tgc
//
LOCUS       DASRWS010000419         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1031648, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000419 DASRWS010000000
VERSION     DASRWS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031648"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VFF72_07865"
     CDS             complement(<1..1391)
                     /locus_tag="VFF72_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZV93118.1"
                     /translation="MILDFIGRHALVLHGMVVAAGLVVYVGASRALPQRRDPSAAIAW
                     VVGLALVPYITLPVYLMFGSRKLRMRDDPAPSPPPLGPPPEASEAASMWARRLGRSMG
                     LAPAARYEALHVHAGGQEALQAALEVIASATRTLDVCTFILAHDAIGMQIAAALRAKA
                     AEGVRVRLLLDGIGAWLGGHADAVRLRGAGVEVVTFVPPFRSILRGRANLRNHRKMIV
                     ADTERLWCGGRNFAEEYFDGPPPLREDGVAATGRDDSAGGSGRKGRPDWRRRAKPGWH
                     DLSFDLSGDIVALACEQFEKDWAYATRAEPAPACAPPAQPLSAEEASLAQLVPSGPDQ
                     VDDTIQSLLLTGCYMARHRIRAVTPYFVPDANLLMALMLAARRGVLIDLVLPARSNHR
                     LADVARHRPLRDLANAGARIWFVPWMTHAKAFVVDEQIALAGSANLDLRSLLLNYELM
                     IAFYERSDVRRYA"
     gene            complement(1473..1889)
                     /locus_tag="VFF72_07870"
     CDS             complement(1473..1889)
                     /locus_tag="VFF72_07870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZV93119.1"
                     /translation="MATLQVDVVSAEEQIFSGEAKFVALPGEMGELGIYPRHTPLITR
                     IRPGAVRIVRADNGEEEFVFVAGGILEIQPGSVTVLADTAIRGKDLDEAKANEAKRAA
                     EEAVKNAANDLDLAKAQNELVALAAQVAALRKYRKK"
     gene            complement(2014..>2488)
                     /locus_tag="VFF72_07875"
     CDS             complement(2014..>2488)
                     /locus_tag="VFF72_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZV93120.1"
                     /translation="TDPSPATTFAHLDSTVVLSRDIASLGIYPAVDPLDSTSRQLDPH
                     VVGEDHYNTARAVQGTLQRYKELRDIIAILGMDELAPEDKLAVARARKIQRFLSQPFH
                     VAEVFTGTPGKYVPLKETIRGFKMIVGGECDSLPEQAFYMVGTIDEAIEKAKTIQ"
BASE COUNT          382 a          839 c          905 g          362 t
ORIGIN      
        1 tcggcatagc ggcgcacgtc gcttcgctcg tagaaggcga tcatcagctc gtagttgagg
       61 agcaggctgc gcaggtcgag gttggccgag ccggccagag cgatctgctc gtcgacgacg
      121 aaggccttgg cgtgcgtcat ccacggcacg aaccagatcc gcgccccggc gttggcgagg
      181 tcgcgcagcg gccggtgccg ggccacgtcg gccagccgat ggttcgaacg cgccggcagc
      241 accaggtcga tcaggacgcc gcgccgcgcc gccagcatca gcgccatcag caggttggcg
      301 tcgggaacga agtacggcgt gacggcgcgg atgcggtgac gcgccatgta gcaaccggtc
      361 agcagcaacg actggatcgt gtcgtcgacc tggtcggggc cgctcggcac gagctgggca
      421 agcgaggctt cctcggccga gagcggctgc gccggtggtg cgcacgcggg cgccggctcg
      481 gcccgcgtgg cgtaggccca gtccttctcg aactgctcgc aggcgagggc gacgatgtcg
      541 ccgctcaggt cgaagctgag gtcgtgccag cccggcttgg cgcggcgccg ccagtcgggc
      601 ctccccttcc gaccggaacc tccggcgctg tcgtcacgac cggtcgcagc cacgccgtcc
      661 tctcgaagcg ggggcggccc gtcgaagtat tcctcggcga agttgcgacc gccgcaccac
      721 agccgctcgg tgtcggcgac gatcatcttg cgatggttgc gcaggttggc gcggccgcgc
      781 aggatcgagc ggaacggtgg cacgaaggtg acgacctcga cgccggcccc gcgcagccga
      841 acggcatcgg cgtggccgcc cagccaggca ccgatgccgt cgagcagcag tcgcacgcgt
      901 accccctcgg ccgccttggc gcgcagcgcc gcggcgatct gcatgccgat cgcgtcgtgg
      961 gcgaggatga acgtgcagac gtcgagcgtg cgcgtcgcac tggcgatgac ctcgagcgcg
     1021 gcctgcagcg cctcctgccc accggcgtgc acgtgcagcg cttcgtagcg cgcggccggc
     1081 gccaggccca tcgatcgacc cagccgacgc gcccacatcg acgccgcctc cgaggcctcc
     1141 ggcggcggcc cgagtggcgg tggcgacggc gccgggtcgt cgcgcatgcg cagcttgcgg
     1201 ctgccgaaca tcaggtacac gggcaaggtg atgtagggca ccagggccag gccgacgacc
     1261 cacgcgatcg ccgccgacgg atcgcggcgc tgcggcaggg cacgcgacgc gccgacgtag
     1321 acgacaagcc ccgcagccac caccatgcca tgcagcacga gggcgtggcg gccgatgaag
     1381 tcgaggatca tgccgggcgc ctcgcggcaa aaacaaaacc cgccgggccg gggccgggcg
     1441 ggctcggcgc gaagccgaag cggttcaccg tgtcacttct tgcggtactt gcgcagcgcg
     1501 gcgacctggg cggcaagcgc cacgagctcg ttctgcgcct tggcgaggtc gagatcattg
     1561 gcggcattct tcaccgcttc ctcggccgcc cgcttggctt cgttggcctt ggcctcgtcg
     1621 agatccttgc cgcggatcgc ggtgtcggcc agcaccgtca ccgagccggg ctggatctcg
     1681 aggatgccgc cggcgacgaa gacgaattcc tcctcgccgt tgtcggcgcg aacgatgcgc
     1741 accgcgccgg gcctgatgcg ggtgatcagc ggcgtgtgcc gaggatagat gccgagctcg
     1801 cccatctcgc ccggcagggc gacgaatttc gcctcgccgg agaagatctg ctcttcggcg
     1861 gagacgacgt cgacttgcag tgtggccatg ctggaaccct ggtgacttga gacgattcag
     1921 gaggcagttg ccggtctcgg agcgaggcgc cgcgcaccgc cgtacgagcg aacggcgagc
     1981 acggcaacga cgcgccggcg ccgaaacgcc ggcctactgg atcgtcttcg ctttttcgat
     2041 ggcttcgtcg atggtgccca ccatgtagaa ggcctgctcg ggaagcgaat cgcactcgcc
     2101 gccgacgatc atcttgaagc cgcggatcgt ttccttcagc ggcacgtact tgcccggcgt
     2161 gccggtgaag acctcggcga cgtggaaggg ctgcgacagg aagcgctgga tcttgcgggc
     2221 ccgggcgacg gcgagcttgt cctccggcgc cagctcgtcc atgccgagga tggcgatgat
     2281 gtcgcgcagc tccttgtagc gctgcagcgt gccctgcacg gcacgcgccg tgttgtagtg
     2341 gtcctcgccg acgacgtgcg ggtcgagctg gcgcgaggtc gagtcgagcg gatcgaccgc
     2401 cgggtagatg cccagcgaag cgatgtcgcg cgacagcacg acggtggagt cgaggtgggc
     2461 gaaggtcgtc gcgggagaag gatcggtc
//
LOCUS       DASRWS010000420         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3906046, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000420 DASRWS010000000
VERSION     DASRWS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906046"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /gene="obgE"
                     /locus_tag="VFF72_07880"
     CDS             complement(<1..762)
                     /gene="obgE"
                     /locus_tag="VFF72_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZV93121.1"
                     /translation="MKFVDEATIEVVAGDGGNGCMSFRREKFIPFGGPNGGDGGRGGS
                     VFAVGDPNLNTLIDYRYARRHEARRGENGRGSDQYGAAAEDIVLRMPMGTIIADAESG
                     DRVAELLVPGEKVCIVRGGDGGFGNLHFKTSTNRAPRQKTPGWPGEQRRLKLELRVLA
                     DVGLLGLPNAGKSTLIAAVSNARPKIADYPFTTLHPNLGVVRVGPEQSFVVADIPGLI
                     EGAAEGAGLGHLFLRHLQRTRLLLHVVDIAPFDESV"
     gene            complement(1052..1309)
                     /gene="rpmA"
                     /locus_tag="VFF72_07885"
     CDS             complement(1052..1309)
                     /gene="rpmA"
                     /locus_tag="VFF72_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZV93122.1"
                     /translation="MAQKKGGGSTRNGRDSQPKMLGVKIFGGQVISAGSIIVRQRGTK
                     FHAGPNVGIGRDHTLFALIDGQVSYAIKGPRNRQTVEVKSI"
     gene            complement(1334..1648)
                     /gene="rplU"
                     /locus_tag="VFF72_07890"
     CDS             complement(1334..1648)
                     /gene="rplU"
                     /locus_tag="VFF72_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZV93123.1"
                     /translation="MYAVIKTGGKQYRVAPGETLKVERIVAEVGAEVRLDQVLGVGVG
                     ADFTAGAPLVSGASVVANVVSHGRHAKVRIFKMRRRKHYQKRQGHRQSFTEIRITAVN
                     PA"
     gene            1813..2766
                     /gene="ispB"
                     /locus_tag="VFF72_07895"
     CDS             1813..2766
                     /gene="ispB"
                     /locus_tag="VFF72_07895"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HZV93124.1"
                     /translation="MPAPVAEEMIEVDAVIRRRLASDVALVDQIANYIISAGGKRIRP
                     MLVLLFSGALGFAGRERFELAATVEFIHTATLLHDDVVDESALRRGRRTANALFGNAA
                     SVLVGDFVYSRAFQMMVSVDRMRILEVLADATNVIAEGEVLQLMNMHDPDLSVEDYLR
                     VIRFKTAKLFEASARLGAVLAEVDTETEEACAAYGRSLGTSFQLVDDLLDYEGASEQL
                     GKNVGDDLREGKPTLPLLIAMERGTPAQRELVRHAIQQGNSDRLSEIVEIVRETGALR
                     ATREAARAEADKAGRSLSLLPDTSYRRALLDLSVRSVERSF"
     gene            2817..2893
                     /locus_tag="VFF72_07900"
     tRNA            2817..2893
                     /locus_tag="VFF72_07900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2851..2853,aa:Pro,seq:cgg)
BASE COUNT          525 a          979 c          882 g          507 t
ORIGIN      
        1 gacgctctcg tcgaacggcg cgatgtcgac aacgtgcaac agcaatcggg tacgttgcag
       61 gtgccgcagg aagagatgcc cgagcccggc gccttcggcg gccccctcga tcaggccggg
      121 gatgtcggcg accacgaaac tctgctcggg tcccacccga acgacgccca ggttgggatg
      181 cagggtcgtg aacggatagt cggcgatctt cggccgggcg ttggagaccg cagcgatgag
      241 tgtcgacttg ccggcattgg gcagaccgag caatccgaca tcggccagga cacggagctc
      301 cagcttcaat cgtcgctgct cgccgggcca tccgggcgtc ttttgccgtg gcgcacgatt
      361 ggtgctcgtc ttgaagtgca ggttgccgaa gccgccatct ccgcccctga cgatgcacac
      421 cttttcgccc ggcaccagca actcggcgac gcgatcaccg ctttcggcat cggcgatgat
      481 cgtgcccatc ggcatgcgca agacgatgtc ctcggccgcc gcgccgtact gatcggagcc
      541 tcggccgttc tcaccccgtc gagcttcgtg ccgcctcgcg tagcgatagt cgatgagggt
      601 gttgaggttc ggatcgccga ccgcgaacac gctgcccccg cgaccgccgt cgcctccgtt
      661 cggaccgccg aacggaatga atttctcgcg tcgaaagctc atgcagccgt tgccgccatc
      721 gccagcgacg acctcgatcg tcgcttcatc gacgaacttc atggatttcg gtccgcgcct
      781 ctcgacggac gacgatcgcc tcgccgcgga ctcaccatcg accagaggcc caatgcgatc
      841 agcaaggccg gccaccactg ctgcagccaa ccccaatgca gccacccgaa attgcctgcc
      901 agaagcaagg cgccgacaac gatgaggatg aaaccgctcc aactcatgcg tgacctcaca
      961 attccccggc cgcaaacgcg caaatggtag tgcaacgcca acgaaaaggc cccgactgcg
     1021 cggggcctcg aggcggcagc gcgaccgcgt gtcagatcga cttgacctcg acggtctgac
     1081 gatttcgagg gcctttgatc gcatagctca cctggccgtc gatcaaggcg aacaacgtgt
     1141 ggtcgcggcc gatgccgacg ttcggtccgg cgtggaactt ggtgccacgc tggcggacga
     1201 tgatcgaacc cgcggaaatg acctgcccgc cgaaaatctt gacgcccagc atcttcggct
     1261 gcgagtcgcg gccattgcgc gtcgagccac cacccttttt ctgtgccatg actgactcct
     1321 tgtccttccc cgatcaggcg ggattgaccg ccgtgatgcg aatctcggtg aacgattggc
     1381 ggtgcccctg gcgcttctgg taatgcttgc gtcgacgcat cttgaagatg cgtaccttgg
     1441 cgtggcgtcc gtgcgagacg acattggcaa cgacgctggc gccacttacc agcggagcgc
     1501 ccgccgtgaa atcggcgccg acgccgacgc cgagaacctg atcgagtcga acttccgccc
     1561 cgacctcggc aacgatgcgc tcgaccttga gagtctctcc gggggcgacc cgatattgct
     1621 tgccgccagt tttgatgact gcgtacatga ccaatccttc aaccggccca aaggccgcgc
     1681 tgcccgagaa aagcctgcga ctataacacg cgctgaattg cacggtggca cacttctata
     1741 atgcgcgacg ctgccagcca gtgcgtcccc gtgcccgcct cgctttcgcc cctcgccgcc
     1801 ccttccctgg gcatgcccgc gcccgtcgca gaagagatga tcgaggtcga cgccgtcatt
     1861 cggcgccggc tcgcctccga tgtcgcgctg gtcgaccaga tcgcgaatta catcatcagc
     1921 gctggcggca agcgcatccg tccgatgctc gtgctgctgt tttcaggggc tctcggattc
     1981 gccggacgcg agcgattcga gctcgcggcc accgtcgaat tcatccacac cgccacgcta
     2041 ctgcacgacg acgtggtcga cgaatcggca ctcaggcgcg gccgacggac ggccaacgcg
     2101 ctcttcggca acgccgccag cgtgctcgtc ggcgatttcg tctattcgcg cgccttccag
     2161 atgatggtct cggtcgatcg catgcgcatc ctcgaggtgc ttgccgacgc cacgaacgtg
     2221 atcgccgagg gcgaggtcct gcaattgatg aacatgcacg accccgacct gagcgtcgaa
     2281 gactatctgc gggtcatccg gttcaagacg gccaagttgt tcgaggccag cgccagactc
     2341 ggggcggtac tggccgaagt cgacaccgaa accgaagagg cgtgcgccgc ctatggccgc
     2401 tcgctcggca catccttcca actggtcgac gaccttctcg actacgaagg tgccagcgag
     2461 cagctcggca agaacgtggg cgacgacctg cgcgaaggca agccgaccct gcccttgctg
     2521 atcgccatgg aacgcggaac acctgctcaa agggagctcg tgcgccatgc gatccagcaa
     2581 ggcaactcgg atcggctctc cgagatcgtc gagatcgttc gcgagaccgg cgcgctgcgg
     2641 gccactcgcg aggcagcccg cgcggaagca gacaaggccg ggcgctcgct ctcgctgctg
     2701 ccggacacga gctaccggcg ggctctgcta gacttgtcgg ttcgatccgt cgagcggtct
     2761 ttttgagttc gacagggatc ggcgacgcgg gcccatggcc tgcacgtcgg actcatcggg
     2821 gtgtagctta gcctggtaga gcgctacgtt cgggacgtag aggccggagg ttcgaatcct
     2881 ctcaccccga cca
//
LOCUS       DASRWS010000421         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3906118, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000421 DASRWS010000000
VERSION     DASRWS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906118"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFF72_07905"
     CDS             <1..255
                     /locus_tag="VFF72_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93125.1"
                     /translation="ADRRIAELMKDRSKLDDEKEFYIGKDLPPQLKARIDANDASLAA
                     RRAARVNLVDDRKRIEKNFDDELATLRKHWRTQPNAGTSG"
     gene            285..620
                     /locus_tag="VFF72_07910"
     CDS             285..620
                     /locus_tag="VFF72_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93126.1"
                     /translation="MTDPAPVSMRHPCLWLLLSGIVASAAATAGPLHPGAAPSAPAGS
                     SGQCNDGTFTSASDIKRACRGHKGVRDWYAAIARRKPGEATISPATAAPPMESTAAHP
                     PVVAGKPPG"
     gene            complement(672..1859)
                     /locus_tag="VFF72_07915"
     CDS             complement(672..1859)
                     /locus_tag="VFF72_07915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZV93127.1"
                     /translation="MRFERSFLSARHLWSSPFVRWQGRLADVSSLDLAVAVTGDALRR
                     LDFDPGRARQIVLGMTIPQPRSFYAAPWIAARLGMAGIGGPHIAQACATSVACVVAAA
                     QSVEGGDEDPHLVVTTDRTSNGPLLVYPRTKGMAGAPSTEHWVLDSFASDPWTGESML
                     CTAEAVARDGAMSREAVDALTLLRFAQYQAALADDRAFQRRWMQPVVIAQGKGRLVVE
                     ADEGVHPYTAEGLASLEPAHKGGVTTFGSQTHPADGCAGALVAGSAAARELAAGDGTV
                     RLLSAASARAEKARMPKAAALAAQRALVDAGIRADQLACVTTHNPFAVNDIWLGQQTG
                     LALELMNPYGCSLIFGHPQAPTGLRSIVELVHALRERGGGVGLFTGCAAGDTGAALVL
                     RVD"
     gene            complement(1873..>2520)
                     /locus_tag="VFF72_07920"
     CDS             complement(1873..>2520)
                     /locus_tag="VFF72_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93128.1"
                     /translation="ESGLGIGHLRETMGLGDVIPAMWSECEAADGMHFNAQRYVAIEL
                     IDPASTEALPWAAGTTGEIVYTAFTREATPLVRYRSRDHALVVGAGRCRCGRTSPRIR
                     CIGRTDDMLIYKAMNVFPTAIRDLVASSFAGRVEPTLRIWKERKEQVRFDEPLPVDVE
                     AAPAVAAQAYEALAAEIEARVRAQLQVRVAVRVLGPGALPRSVYKSSLFAVRDGT"
BASE COUNT          400 a          886 c          886 g          348 t
ORIGIN      
        1 gccgatcgcc gcatcgccga attgatgaag gatcgatcga agctcgacga cgaaaaggag
       61 ttctacatcg gcaaggacct gccgccgcaa ctcaaggcga ggatcgatgc caacgacgcg
      121 tcgctggcgg cgcgtcgtgc cgcacgcgtc aacctcgtcg acgaccgcaa gcggatcgag
      181 aagaatttcg acgatgagct ggcgacgctg cgaaagcatt ggcggacgca accgaacgcc
      241 ggcacgtccg ggtgaagaac acgcagcctg gcgaagagac gcacatgacc gacccggccc
      301 ctgtctcgat gcggcatccc tgcctttggc tcctgctcag cggcatcgtc gcgtcggcgg
      361 ccgcgaccgc cggtccgctc catcccggcg ccgcgccgag cgctccggcc ggctcgtcgg
      421 gccagtgcaa tgacggcacg ttcacctcgg ccagcgacat caagcgcgcc tgtcgcggcc
      481 acaagggcgt cagggactgg tacgccgcga tcgcaaggcg caagccgggc gaggcgacga
      541 tttctccggc gaccgccgcg ccgccgatgg aaagcaccgc ggcgcatccg ccggtcgtcg
      601 ccggcaagcc cccgggctga acgtcgacgc tgcgcagcgc cgccgagcga aagcgcttcg
      661 cccggccggg ctcagtcgac gcgcaggacc agggccgcgc cggtgtcgcc ggcggcgcag
      721 ccggtgaaca ggccgacgcc gccgccgcgc tcgcgaagcg catgcacgag ctcgacgatc
      781 gatcgcagtc ccgtcggcgc ctgggggtgg ccgaagatca ggctgcagcc gtacggattc
      841 atgagctcga gcgcgagacc ggtctgctgc cccagccaga tgtcgttgac ggcgaagggg
      901 ttgtgcgtcg tcacgcaggc gagctggtcg gcacgaatgc cggcgtcgac gagggcgcgc
      961 tgagcggcca gcgccgccgc cttcggcatg cgtgccttct ccgcgcgcgc ggaggctgcc
     1021 gagagcaatc gaaccgtccc gtcgccggcc gcgagctctc gtgcggcggc gctgccggca
     1081 accagcgccc cggcgcaacc atcggccggg tgcgtctgcg aaccgaaggt cgtgacgccg
     1141 cccttgtgcg ccggctcgag cgacgcgaga ccttcggcgg tgtacggatg gacgccttcg
     1201 tcggcttcga cgacgagtcg tcccttgccc tgggcgatga cgacgggctg catccagcga
     1261 cgctggaagg cccggtcgtc ggcgagtgcg gcctggtact gggcgaagcg aagcagcgtc
     1321 agcgcatcga ccgcctcgcg cgacatggcg ccgtcgcggg cgacggcttc ggccgtgcac
     1381 agcatcgact cgccggtcca cggatcgctg gcgaagctgt cgagcaccca atgctcggtc
     1441 gacggtgcgc cggccatgcc cttcgttctc ggatagacga gcagcggccc gttgctggtg
     1501 cggtcggtcg tgacgacgag atgcggatct tcgtcgccgc cctcgaccga ttgcgccgcg
     1561 gcgacgacgc aggccaccga agtcgcgcag gcctgggcga tgtgcgggcc gccgatgcct
     1621 gccatgccga gccgggcggc gatccacggc gcggcgtaga agctgcgcgg ctgcgggatc
     1681 gtcatgccga gaacgatctg ccgggctcga ccgggatcga agtcgaggcg tcgcagcgca
     1741 tcgcccgtga ccgccaccgc gagatcgagg ctcgacacgt cggcgagacg gccctgccag
     1801 cgaacgaacg gcgacgacca cagatggcgc gcgctcagga agcttcgctc gaatcgcatc
     1861 gtccctcttt cttcatgtgc cgtcgcgaac ggcgaacagc gaactcttgt agacgctgcg
     1921 cggcagcgcg ccggggccga gcacccgcac cgcgacgcgg acctgcaatt gcgcgcgaac
     1981 gcgtgcttct atctccgcgg cgagtgcctc gtaggcctgc gccgcgaccg ccggcgccgc
     2041 ctcgacgtcg accggcaacg gctcgtcgaa gcgcacctgc tcctttcgct ccttccagat
     2101 ccgcagcgtg ggctcgaccc gcccggcgaa cgacgacgcg acgagatcgc ggatcgctgt
     2161 cggaaagacg ttcatcgcct tgtagatgag catgtcgtcg gtgcggccga tgcagcggat
     2221 ccgcgggctg gtgcgcccgc aacggcaacg accggcgccg acgacgagcg catggtcgcg
     2281 cgagcggtag cgcaccagcg gcgtcgcttc gcgggtgaag gcggtgtaga cgatctcgcc
     2341 ggtcgtgcct gcggcccacg gcagcgcctc ggtgctcgcc gggtcgatca gttcgatggc
     2401 gacgtagcgc tgggcattga agtgcatgcc gtcggcagcc tcgcattccg accacatcgc
     2461 cggaatgacg tcgcccaggc ccatcgtttc gcgcagatgc ccgatgccca ggccggactc
//
LOCUS       DASRWS010000422         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_469863, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000422 DASRWS010000000
VERSION     DASRWS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469863"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VFF72_07925"
     CDS             complement(<1..1665)
                     /locus_tag="VFF72_07925"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HZV93129.1"
                     /translation="MSHVPTSRGCRRGATLGLLALVALLATACGGGSSPDAAPVASKE
                     TALAASAPGELLAYVKTLLLARDAQRQAAPTVSLDGPPLPGASLALSATGAAVPYSNT
                     TVQEAGVDEEDLLKTDGTSVYALDTASTIVNGRLQQQLLVSRRDAGGGIDPVQVLPLP
                     VDTTGFAQPQGLLLAAPARRAVALSQSLLPVADPLPCAVDFACPLAGASIYPAFALQS
                     NVQLQFFDLDATGNATLGKHVAIDGQFVGSRLIGNVMVLVTTYSPRVPFELLPLGTPT
                     AQKASTLAQMSTADFMPSWRADGAAPEALVADTDCYVHPSNASLAFQVTTITTIDLSS
                     QTLARKSRCFIGGTEAIYMAPENLYLATTRFPYVTLSSGLLQYAPQFATDIHKFSVDG
                     TDIDYRASGEVQGNLGWDTQRKPYRMSEYNGDLRVLSYTAPTGWVLLPDALSPSAPPP
                     SPATLTILRESASGNTLDTVATLPDSAHPEPLGEPGEQVYAVRFTGARGYLVTFRSVD
                     PLYVLDLSDPTDPHVAGSLQVTGFSDYLFPLTDSLLFGVGRDVDPAS"
     gene            1748..2584
                     /locus_tag="VFF72_07930"
     CDS             1748..2584
                     /locus_tag="VFF72_07930"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HZV93130.1"
                     /translation="MTSRHSIVEAGVLRVMRPLARLLVRHGVTYPAFAAALKRVFLDA
                     ATDELEARGMAQTDSAVSLLSGVHRRDVRNFTRAPERASPAARGKRAGAAPLSLVGEV
                     VARWLTDAAWQDRRRRPRALQRTEFDALVAAVSSDVRGRAMLDELVRLGAVVEGADGI
                     ALASDGFAPRQGFAEMSELFAANLAYHASAASANLQGEANFLEQAMYVDRLTPESVQR
                     LQRVARRAWTKALPPVLAEAQACSDADVAKASAAGDAATAGAGQRVRFGVYFYSTEGA
                     EP"
     gene            2581..3117
                     /locus_tag="VFF72_07935"
     CDS             2581..3117
                     /locus_tag="VFF72_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93131.1"
                     /translation="MRSELRRAWLASAWILGALLCAGCGPGVGGTGTGNDAAFAAFGA
                     NAGPVCSATVASELACPSAPAGPVPPTGTLPVRFADAAGQVVLELNGNVAQLDDSCLR
                     LHFSGEFGTTAGGAQGFFGSYEIGGNGVDMLAALSIAPVAGGGSLTVGLRGVDGQMVV
                     GPLPLQRATGPLPAPAPC"
     gene            3233..3703
                     /locus_tag="VFF72_07940"
     CDS             3233..3703
                     /locus_tag="VFF72_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZV93132.1"
                     /translation="MRYNSTTLGVAIVAAFVAGVVASPLAQHAIGDARAEAAPLEPMM
                     IDLMAIKHADLPTTSNPEMNAKSLVVTDNATIGIQSGNVAKHMHPKTNEIQYIIEGSG
                     AMWLGGERKEFKPGTLIIISKGTAHGGTLVTSGPVKALAIKIPPQGAGDTVFLN"
     gene            complement(3728..3994)
                     /locus_tag="VFF72_07945"
     CDS             complement(3728..3994)
                     /locus_tag="VFF72_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93133.1"
                     /translation="MTWETRQALIAESNRLLAMRAARPGSRSRPERHHAQDSAEDTSF
                     APDGDEFAPDTGADSEAGLSSTLSGGVDDLPSTAAATSGSEVPN"
     gene            complement(4118..4597)
                     /locus_tag="VFF72_07950"
     CDS             complement(4118..4597)
                     /locus_tag="VFF72_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV93134.1"
                     /translation="MTAKNTICLWFDRDAEAAATFYAATFPDSAVTAVRRAPADYPNG
                     RKGDVLTVDFTVAGIPCLGLNGGPAFKQTEAFSFQIATDDQAETDRLWNAIVGNGGQE
                     SACGWCKDRWGVSWQITPRALSAGMADPDPAVARRVFESMMTMRKIDVAAIEAARRG"
     gene            complement(4594..5598)
                     /locus_tag="VFF72_07955"
     CDS             complement(4594..5598)
                     /locus_tag="VFF72_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZV93135.1"
                     /translation="MDHLPLVDLRTPPDDVARAIGTACRAHGFFYVVNHGIDESLGQR
                     LESLSHAFFALPEEVKARYAMPLGGRAWRGWFPLGGELTSGRPDWKEGLYLGTELASA
                     HPRVAAGVPLHGANLIPGDDVLPGFRQTILDWMSEVTALGHRVMEGIARSLDLPADYF
                     ARRYTADPLILFRIFQYPSRAVPEGMDVRHGVGEHTDYGLLTLLRQDDIGGLQVHTKE
                     GWIEAPPVPGSFVCNIGDMLDRMTGGLYRSTPHRVRLNTSGRHRLSFPLFFDPDFDSR
                     IEPIRSAATDDHATRWDGASVHAFEGTYGDYVMGKVGKVFPDLRREVQLDSATPQKTT
                     "
     gene            complement(5668..>6704)
                     /locus_tag="VFF72_07960"
     CDS             complement(5668..>6704)
                     /locus_tag="VFF72_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HZV93136.1"
                     /translation="PYRDRGSDITVLTSLAARRDCTSRTRSDIDTELRKIRAGQKGEK
                     DAAYAIDFFLAQRDEWAVIHDLRIEWQGRVAQIDHLFINRLMDVWVCESKHFSEGVSI
                     NEHGEFTAFYNNRPRGIESPIEQNQRHIKVLESVLGSSILPLPTRLGIPLRSTIRSVV
                     LVSAAARITRPKQTFEGLETVIKADQLRSRINQAIDAMGVGETLLKATKMVGTETLRQ
                     FASDLGKLHRPQQFDWAARFGMSAHEPMAVRASAAPVRQTQTRTQVAAEPAGAAKISS
                     SKLASLLGLPSARALLERLVAEGFLVPTHQGHALTEAGLAAGGEFVEKSRYGPYFVWP
                     PALQLRERQT"
BASE COUNT         1011 a         2284 c         2376 g         1033 t
ORIGIN      
        1 gctcgccggg tcgacgtcgc ggccaacgcc gaagagaagc gagtcggtca gcggaaacag
       61 gtagtcggaa aagcccgtca cctgcagcga gccggccacg tgcggatcgg tcgggtcgct
      121 caggtcgagc acgtagagcg ggtcgacgct gcggaaggtg acgagatagc cgcgcgcgcc
      181 ggtgaagcgc accgcgtaga cctgctcgcc gggctcgccg agcggctcgg ggtgggcgct
      241 gtcgggcaac gtggcgacgg tgtcgagcgt gttgccgctg gccgattcgc gaaggatcgt
      301 cagcgtcgcc ggcgacggcg gcggcgcgct gggcgagagc gcgtcgggca gcagcaccca
      361 gccggtcggc gccgtgtagc tcagcacgcg caggtcgccg ttgtattcgc tcatccggta
      421 gggcttgcgt tgcgtgtccc agccgaggtt gccctgcacc tcgcccgagg cgcggtagtc
      481 gatgtcggtg ccgtcgaccg agaacttgtg gatgtcggtc gcgaattgcg gtgcgtactg
      541 gagcagcccg ctcgacaggg tgacgtaggg aaagcgcgtc gtcgccaggt agaggttttc
      601 cggggccatg tagatcgcct cggtgccgcc gatgaagcag cggctcttgc gcgcgagcgt
      661 ctgcgacgac aggtcgatgg tcgtgatcgt cgtcacctgg aaggcgagcg acgcgttcga
      721 aggatggacg tagcagtcgg tgtcggccac cagcgcttcc ggcgccgcgc cgtcggcccg
      781 ccacgacggc atgaagtcgg ccgtgctcat ctgcgcgagc gtgctcgcct tctgcgctgt
      841 cggcgtgccc agcggcagca gctcgaaggg cacgcgcggc gagtaggtcg tgacgaggac
      901 catgacgttg ccgatcaggc ggctgccgac gaactggccg tcgatcgcga cgtgcttgcc
      961 gagcgtggcg ttgccggtgg cgtcgagatc gaagaactgc agctgcacgt tcgactgcag
     1021 cgcgaaggcc ggatagatcg acgcgccggc gagcgggcag gcgaagtcga ccgcgcacgg
     1081 cagcgggtcg gccaccggca gcaacgactg gctcagcgcc acggcgcgcc gggccggcgc
     1141 cgccagcagc agcccctgcg gttgcgcgaa gcccgtcgtg tcgaccggca gcggcagcac
     1201 ctgcaccggg tcgatgccgc cgccagcgtc gcggcgcgag acgaggagct gctgctgcag
     1261 ccgcccgttg acgatggtcg acgcggtgtc gagcgcgtag accgacgtgc cgtcggtctt
     1321 cagcaggtct tcctcgtcga cgcccgcttc ctgcaccgtg gtgttcgagt acggcacggc
     1381 cgcgccggtc gcgctcaggg ccagcgaggc gccgggcaac ggcggcccgt cgagcgacac
     1441 ggtgggcgcc gcctggcgct gggcgtcgcg cgccagcagc agcgtcttca cgtaggcgag
     1501 cagttcgccc ggcgccgatg ccgcgagcgc ggtctccttc gacgccaccg gcgccgcgtc
     1561 gggcgacgag ccgccgccgc aggccgtggc caggagcgcc accagggcga gcaggccgag
     1621 cgtcgcgccg cgacggcaac cgcgtgatgt ggggacgtgc gacatggcgc ctccttgtcg
     1681 atccgatgtc tgtgtgggac aatatcccac gccaatccga tccgtcaagc ccggacagca
     1741 ccccttcgtg accagccgtc actcgatcgt cgaagccggc gtcctgcgcg tgatgcggcc
     1801 gctggcgcgg ctgctggtgc gccacggcgt cacctacccg gccttcgcgg cggcgctcaa
     1861 gcgcgtcttc ctcgacgccg ccaccgacga gctcgaggcg cgcggcatgg cgcagaccga
     1921 cagcgccgtc tcgctcctgt cgggcgtgca ccggcgcgac gtccgcaact tcacgcgggc
     1981 tcccgaacgc gcctcgccgg ccgcgcgcgg caaacgcgcc ggcgccgcgc cgctctcgct
     2041 cgtcggcgaa gtggtcgcgc gctggctcac cgacgccgcc tggcaggatc ggcggcgccg
     2101 tccccgcgcc ctgcagcgca ccgagttcga cgccctggtc gccgccgtca gcagcgacgt
     2161 gcgcggccgc gccatgctcg acgagctggt gcggctgggc gcggtcgtcg aaggcgccga
     2221 cggcatcgcc ctcgcgtcgg acggcttcgc gccgcgccag ggcttcgccg agatgtccga
     2281 gctgttcgcc gccaacctcg cctaccacgc ctcggcggcg tcggccaacc tgcagggcga
     2341 agccaatttc ctcgagcagg cgatgtacgt cgatcgcctc acgcccgagt cggtgcaacg
     2401 actgcagcgg gtcgcccggc gcgcctggac gaaagccttg ccgccggtgc tggccgaggc
     2461 ccaggcctgc agcgatgccg acgttgccaa ggcgagcgcg gccggcgatg cggccaccgc
     2521 aggtgcgggc cagcgggtgc gcttcggcgt ctacttctac agcaccgaag gagctgaacc
     2581 gtgagatccg aacttcggcg agcctggctc gccagcgcct ggattctcgg agcgctcctc
     2641 tgcgcaggct gcggccccgg cgtgggtggc accggcaccg gcaacgacgc ggcctttgcc
     2701 gccttcggcg cgaacgccgg cccggtgtgc agtgcgaccg tcgcctccga gctcgcctgc
     2761 ccgtccgcgc cggccggccc ggtgccgccc accggcacgc tgccggttcg attcgccgac
     2821 gcggccggtc aggtcgtgct cgagctgaac ggcaacgtcg cgcagctcga cgactcgtgc
     2881 ctgcgcctgc acttcagcgg cgaattcggc acgaccgcgg gcggcgccca gggcttcttc
     2941 ggcagctacg agatcggcgg caacggcgtc gacatgcttg ccgcgctctc gatcgcgccg
     3001 gttgccggcg gcggctcgct caccgtcggc ctgcgcggcg tcgacgggca gatggtggtc
     3061 ggcccgctgc cgttgcaacg ggcgaccggc ccgctgccgg cgccggcgcc ctgttgaccg
     3121 gcgggcgaca cggcgtccgg tcgccgatca aggcagaccc ttattcgcgc ccgagatcgg
     3181 cgaccggata ctggttcgcg cagtttcgcg cgtactcccg aaggacggtc ccatgcgcta
     3241 caactcgacg accctgggtg tcgccatcgt cgcggccttc gtcgccggcg tcgtcgcctc
     3301 gccgctcgcg caacacgcga tcggcgatgc ccgcgccgag gccgcgcctc tcgagccgat
     3361 gatgatcgac ctcatggcga tcaagcacgc cgacctgccg accacgtcga accccgagat
     3421 gaacgccaaa tcgctggtcg tcaccgacaa cgcgacgatc ggcatccagt cgggcaacgt
     3481 ggccaagcac atgcacccga agaccaacga gattcaatac atcatcgaag gcagcggcgc
     3541 gatgtggctc ggcggcgagc gcaaggagtt caagccggga acgctgatca tcatttccaa
     3601 gggcaccgcg cacggcggca cgctggtgac cagcgggccg gtcaaggcgc tggccatcaa
     3661 gattccgccg cagggcgccg gcgataccgt gttcctgaac tgagccgcgc gaaaggcgcg
     3721 gccgaggtca gttcggcact tcgctgccag aggtggccgc cgccgtcgat ggcagatcgt
     3781 ccacgccgcc ggatagcgtg ctcgagaggc ccgcttccga gtcggcgccc gtgtcgggtg
     3841 cgaactcgtc cccgtcgggc gcgaacgatg tgtcttcggc gctgtcctgg gcgtggtgcc
     3901 tttccggtcg tgagcgggat ccaggccgtg cggcccgcat ggcgaggagg cggttcgatt
     3961 cggcgatcag cgcctggcgc gtctcccagg tcatgtagaa gggaagcatc gaagtgaggg
     4021 cgagtggcga tgtcgccaag gtagcgagaa gcgcccgcca cccgcgtccg cctgcatcgc
     4081 cctgccgtgt cggacaagca cgcgcgagga acggcgatca accccgccgc gccgcctcga
     4141 tcgccgcgac gtcgatcttc ctcatcgtca tcatcgactc gaacacgcgc cgggccaccg
     4201 ccgggtcggg gtcggccatg cccgcgctga gggcacgcgg cgtgatctgc cacgacacgc
     4261 cccagcggtc cttgcaccag ccgcaggcgc tctcctggcc gccgttgccg acgatcgcgt
     4321 tccacagccg gtcggtctcg gcctgatcgt cggtggcgat ctggaacgag aacgcctccg
     4381 tctgcttgaa cgcggggccg ccgttgaggc cgaggcacgg aatgccggcc acggtgaagt
     4441 cgaccgtgag cacgtcgccc ttcctgccgt tcgggtagtc ggccggcgca cggcgaacgg
     4501 ccgtgacggc gctgtcggga aaggtggcgg cgtagaacgt cgccgcggcc tcggcgtcgc
     4561 ggtcgaacca gaggcagatg gtgttcttgg cggtcatgtc gttttctgcg gagtggcgga
     4621 atccagttgt acctcgcgcc gcaagtccgg aaacaccttg ccgaccttgc ccatcacgta
     4681 gtcgccgtac gtgccctcga aggcgtgcac gctcgcgccg tcccagcgcg tggcgtggtc
     4741 gtcggtggcg gcgctgcgga tgggctcgat gcgcgaatcg aaatcggggt cgaagaagag
     4801 cgggaacgag aggcggtgcc ggccgctcgt gttgaggcgc acgcgatgcg gcgtgcttcg
     4861 atagaggccg ccggtcatgc ggtcgagcat gtcgccgatg ttgcagacga acgaccccgg
     4921 caccggcggc gcctcgatcc agccttcctt cgtgtgcacc tgcaggccgc cgatatcgtc
     4981 ctgccgcagc agcgtgagca ggccgtagtc ggtgtgctcg cccacgccgt ggcgcacgtc
     5041 catgccttcg ggcacggcgc ggctcgggta ctggaagatg cgaaagagga tgagcggatc
     5101 ggccgtgtag cggcgcgcga agtagtcggc cggcagatcg agcgagcggg cgatgccctc
     5161 catcacgcgg tggccgagcg ccgtgacctc gctcatccag tcgaggatcg tctgcctgaa
     5221 gccgggcagc acgtcgtcgc cggggatgag gttggcgccg tgcagcggca cgcccgcggc
     5281 cacgcgcggg tgcgccgagg ccagctcggt gccgaggtac aggccctctt tccagtcggg
     5341 ccggcccgag gtgagctcgc cgccgagcgg aaaccagccg cgccaggcgc ggccgccgag
     5401 cggcatggcg tagcgcgcct tgacctcttc ggggagggcg aagaacgcgt ggctcaggga
     5461 ttcgaggcgc tggccgaggg actcgtcgat gccgtggttg acgacgtaga aaaagccgtg
     5521 ggcgcggcag gcggtgccga tggcgcgggc tacgtcgtcg ggcggcgtac ggaggtcgac
     5581 aagcggaagg tggtccatcg ccggcggatg gtagaccggc accaatcaac gcccgcgcgg
     5641 atagcggccg ggaagatggc cgcgtcgcta ggtttgacgc tcccgcagtt gcaacgcggg
     5701 gggccagacg aaataagggc cataccgact cttctcgacg aactctccac cggcagccag
     5761 gccggcctcg gtcagcgcgt gcccctggtg cgttggaacg agaaacccct ctgcaaccag
     5821 ccgctcgagc aacgctcggg cgctcggaag gccgagaagc gaagcgagct tcgagctgga
     5881 aatcttcgct gcgcccgccg gttcggcagc gacctgcgtc cgggtctgcg tctgcctgac
     5941 cggcgcggcc gaggcccgca ccgccattgg ttcatgtgcc gacatgccaa agcgtgccgc
     6001 ccaatcgaat tgctgcggcc gatggagctt tcccagatcg ctcgcgaact gccgcaatgt
     6061 ttcggttccg accatcttcg ttgccttcag aagcgtctcg ccgacaccca tcgcgtcgat
     6121 ggcctggttg atcctgctgc gcaactgatc tgccttgatc accgtctcaa ggccttcgaa
     6181 ggtttgcttc ggcctcgtga tacgcgcagc agcgctcacg agaacgacgc ttcgaatcgt
     6241 cgagcggagt gggattccga gccgcgtcgg caacggcagg atgctgctgc ccaacacgct
     6301 ttcgaggacc ttgatatgac gctggttctg ctcgatgggc gactcgatgc cgcgcggccg
     6361 attgttgtag aacgcggtga attcgccatg ctcgttgatc gagacaccct cgctgaagtg
     6421 cttcgactcg cagacccaca cgtccatgag acggttgatg aacaaatggt cgatctgcgc
     6481 cactcgaccc tgccattcga tcctcaggtc gtgaatgacg gcccactcgt ctctttgcgc
     6541 cagaaaaaag tcaatggcgt aggcagcgtc cttttcgcct ttttgaccgg cgcgaatttt
     6601 tcgcagctcg gtgtcgatgt cgctccttgt acgagacgta cagtcacgac gcgcagcgag
     6661 cgaggtcaag actgtgatat cgctgccgcg gtctcggtaa ggct
//
LOCUS       DASRWS010000423         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1321893, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000423 DASRWS010000000
VERSION     DASRWS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321893"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFF72_07965"
     CDS             <1..1031
                     /locus_tag="VFF72_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZV93137.1"
                     /translation="PSLWRQEFEQTGAGDYPTSEPEVKAMVDFIVRHPNIGAAISYHT
                     HSGVILRPMGTQSDDDMIPEDLWSIKRFSELGTKLTGYPAISIWHEFKYHPKEVISGT
                     QDWLYEHLGALFWVVEIWSPNREAGITGYKWIDWYREHPVEDDLKLIKWSDEQCGGKA
                     HVEWKPFMHPQLGQIEIGGWDKMNYWRNPPPQLREREAARFPKWMTQIALSLPRLELL
                     RTEVRALGPDTWRVKLAVANSGWLPAYVTKRALARKTVRGVVFEMHLPDGDPSVSLVS
                     GKPRMEGPQLEGHAPKSSLNAFLPNREVTADRAVAEWVVRAPKGTRLALSARAARAGA
                     VRAEVILD"
     gene            complement(1110..2561)
                     /locus_tag="VFF72_07970"
     CDS             complement(1110..2561)
                     /locus_tag="VFF72_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZV93138.1"
                     /translation="MSLNVVTLLYLVASVCFIQALKGLSHPTTSIRGNAFGMTGMAIA
                     AFTTAALIVKLVDSYGLSPAAGLGWVLLGLVVGGGYGAWRARTVEMTAMPELVAFFHS
                     MIGLAAVFIAIAAATEPYAFGIVARPLAGGAVDPIPIGNRLELILGAAIGAITFSGSV
                     IAFGKLSGKYKLRVFRGAPVVFAGQHWVNLLLAIAMAVFIVGFTLTQSPIDFYVALAI
                     AFVLGVLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAIL
                     SYIMCKAMNRAFINVILGGFGGEGGPAAGQVEQRPVKSGSADDAAFVLGNAETVIIVP
                     GYGLAVARAQHAVKELAAKLTEKGVKVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFE
                     MDDINGEFGQADVAIILGANDVVNPAAHTKGSPIYGMPILEASKAKTVIVNKRSMAAG
                     YAGLDNELFYMDKTMMVFGDAKKVVEEMVKAIE"
     gene            complement(2641..2967)
                     /locus_tag="VFF72_07975"
     CDS             complement(2641..2967)
                     /locus_tag="VFF72_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZV93139.1"
                     /translation="MDVVSHTVINLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTETTLGKTMGVLAVALASVNIFGGFLVTRRMLEMFRKKDKPKPAT
                     SPTAAK"
     gene            complement(2998..4128)
                     /locus_tag="VFF72_07980"
     CDS             complement(2998..4128)
                     /locus_tag="VFF72_07980"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZV93140.1"
                     /translation="MLIGVPRETAIGETRVAVTPETAKKLAAQGHSLRVESGAGVAAS
                     ATDDAYRAVGAEIADRAATFASDLVLKVRSPDAGELTLMKPGTHLVGMLNPFDRAGLD
                     ALAAAGLTSFALEAAPRTTRAQSMDVLSSQANIAGYKAVMVAADTYQRFFPMLMTAAG
                     TVKAARVVVIGVGVAGLQAIATAKRLGAVIEASDVRPSVKEQVESLGAKFIEVPYETA
                     EEKEAAEGTGGYARAMPASWLDRQKVEVAKRVALADVVISTALIPGRAAPVLVTEEMV
                     RSMKPGSVIVDLAAGKGPDGHGGNCPLSVADRTVVVHGVTLIGETNLASKVGADASSL
                     YARNVLDFLKLVLPKEGGLVVPMDDDIVVACLMTQGGVVKRQ"
     gene            4244..4753
                     /locus_tag="VFF72_07985"
     CDS             4244..4753
                     /locus_tag="VFF72_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZV93141.1"
                     /translation="MDERWKPSVTVAAIASRQAGGRREYLVVEEETADGLRLNNPAGH
                     LEPGESLQQAVVREALEETGRCYAPSRLVGVYLARVRLAGDARDDATYLRFAFAGTAG
                     DADPQRRLDKGIVGTLWLTLDELRRSRHRHRSPLVLRCVEDDLAGRHVPLDVLSTDLG
                     LAGGPRTAA"
     gene            4782..5471
                     /locus_tag="VFF72_07990"
     CDS             4782..5471
                     /locus_tag="VFF72_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV93142.1"
                     /translation="MASRLLIAEDDYAIATNLFTYFERKGYDVEAVYSGQAAMHRCSV
                     ERFDLVILDIGLPGLDGLTFLQRLRGELRVATPVLVVSARSDLSDKLAGFEHGADDYV
                     TKPFALAEVEARVRALLNRARGASIDPVRRLGRLAFDVREGEARVGDALVRLTPKAAQ
                     LLDLLLRYPGQLVRRAEIEKALWDDEVANADALRSQVHWLRRALAEAGFDGIETVHGV
                     GYRIVERPSAP"
     gene            5468..>5855
                     /locus_tag="VFF72_07995"
     CDS             5468..>5855
                     /locus_tag="VFF72_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93143.1"
                     /translation="MIALSRRRFTLTQRVVWAVTATAALLLGLQSVLAYFAMHAQEDE
                     LSDDMLRREVQQLITLTLKPGLMPVGSLAASPGLTAYLTRGSEGMASVPDDVRRLEPG
                     LYQLAKDGTVRHVAVVDTEDGRLRIVL"
BASE COUNT          917 a         2051 c         1988 g          899 t
ORIGIN      
        1 ttccgtcgct ctggcgacag gagttcgagc agaccggcgc gggcgactac ccgaccagcg
       61 agccggaagt gaaggcgatg gtcgacttca tcgtccgcca tccgaacatc ggcgcggcga
      121 tcagctatca cacgcacagc ggcgtcatcc tgcggccgat gggaacgcaa agcgacgacg
      181 acatgattcc cgaggacctg tggtcgatca agcgcttcag cgagctcggc acgaagctca
      241 cgggctatcc ggcgatcagc atctggcacg agttcaagta ccacccgaag gaagtcatct
      301 ccggcaccca ggactggctc tacgagcacc tgggcgcgct cttctgggtg gtcgaaatct
      361 ggtcgccgaa ccgggaggcc ggcatcaccg gctacaagtg gatcgactgg taccgcgagc
      421 atccggtcga ggacgacctc aagctcatca agtggagcga cgagcagtgc ggcggcaagg
      481 cacatgtcga atggaaaccc ttcatgcacc cgcagctggg ccagatcgag atcggcggct
      541 gggacaagat gaactactgg cgcaacccgc cgccgcagct tcgcgagcgc gaggcggcgc
      601 gctttccgaa gtggatgacg cagatcgccc tgtcgctgcc caggctcgag ctgctgcgca
      661 ccgaagtgcg ggcgctcggc cccgacacct ggcgcgtcaa gctcgccgtg gccaacagcg
      721 gctggctgcc ggcgtacgtg acgaagcggg cgctggcgcg aaagaccgtg cgcggcgtcg
      781 tcttcgagat gcatctgccc gatggcgacc cttcggtgtc gctggtgagc ggcaagccgc
      841 gaatggaagg gccccagctc gaggggcacg cgccgaagtc ttcgctcaac gcattcctgc
      901 ccaatcggga agtcacggcc gaccgcgcgg tggccgaatg ggtcgtgcgg gcgccgaagg
      961 gaacgcggct cgcgctcagc gcccgcgccg cgcgcgccgg agcggttcgc gccgaggtga
     1021 tcctcgactg agcggtgctg ccgcttccag cgctgccgcg atgcgccagc ggcgttgccg
     1081 cttcgacgtg acgggcaccg cgcgccggcc tactcgatcg ccttgaccat ctcctcgacg
     1141 accttcttgg cgtcgccgaa gaccatcatc gtcttgtcca tgtagaagag ctcgttgtcg
     1201 aggccggcgt agccggcggc catcgagcgc ttgttgacga tcacggtctt ggccttcgac
     1261 gcctcgagga tcggcatgcc gtagatcggg ctgcccttgg tatgcgcggc cgggttcacg
     1321 acgtcgttgg cgccgaggat gatcgcgaca tcggcctggc cgaactcgcc gttgatgtcg
     1381 tccatctcga agacctggtc gtaaggcacc tcggcctcgg cgagcagcac gttcatgtgg
     1441 cccggcatcc ggccggcgac cggatggatc gcgtacttga ccttgacgcc cttctcggtg
     1501 agcttggcgg ccagctcctt gaccgcatgc tgggcccgcg ccacggccag gccgtagccg
     1561 ggcacgatga tcaccgtctc ggcattgccg aggacgaatg cggcatcgtc ggcactgccg
     1621 ctcttcaccg gccgctgctc gacctgccct gccgccgggc cgccctcgcc accgaagccg
     1681 ccgaggatga cgttgatgaa cgcacggttc atcgccttgc acatgatgta gctgaggatc
     1741 gcgcccgacg agccgaccag cgagccggcg atgatcagca tcgcgttgtt gagcgagaag
     1801 ccgatccccg cggcggccca gcccgagtag ctgttgagca tcgacaccac gaccggcatg
     1861 tcggcgccgc cgatcgggat gatgatgagc acgcccagca cgaacgcgat cgccagggcg
     1921 acgtagaagt cgatcgggct ttgcgtcagc gtgaagccga cgatgaagac ggccatcgcg
     1981 atcgcgagga ggagattgac ccagtgctgt ccggcgaaga cgacgggtgc gccgcggaag
     2041 acgcgcagct tgtacttgcc tgagagcttg ccgaaggcga tcaccgagcc gctgaaggtg
     2101 atcgcgccga tcgccgcgcc gaggatcagc tcgagccggt tgccgatcgg gatcgggtcg
     2161 accgcgccgc ccgccagcgg ccttgcgacg atgccgaatg cgtacggctc ggtcgccgcg
     2221 gcgatggcga tgaacaccgc cgccaggccg atcatgctgt ggaagaacgc caccagctcg
     2281 ggcatcgccg tcatctcgac cgtgcgtgcc cgccacgcgc cgtagccgcc gccgacgacc
     2341 aggccgagca gcacccagcc caagcccgcg gccggcgaca ggccgtacga gtcgaccagc
     2401 ttgacgatca gcgcggcggt cgtgaacgcg gcgatcgcca tgccggtcat gccgaaggcg
     2461 ttgccgcgga tcgacgtcgt cgggtggctg agccctttga gcgcctggat gaagcagacg
     2521 ctggcgacga ggtagagcag cgtgacgacg ttgaggctca tcgcgcctcc gcgttgccgc
     2581 cgcacaccgc cgcgccgccc cggcaggcca ggttcgcgcc cggcaaggaa gcgccggctc
     2641 tcatttggcc gccgtcggcg aagtcgccgg cttcggcttg tccttcttcc tgaacatctc
     2701 gagcatgcgc cgagtcacca ggaagccgcc gaagatgttg accgacgcca gcgccacggc
     2761 gagcacgccc atcgtcttgc cgagcgtcgt ctcggtgagc gccgccgcga gcatcgcgcc
     2821 gacgatgacg atcgccgaga tggcgttggt caccgccatc agcggcgtgt gcagcgccgg
     2881 cgtcaccgtc cagacgacgt ggtagccgac gtagatcgcg agcacgaaga tgatcaggtt
     2941 gatgacggta tgggaaacga cgtccatggc ttcggctttc cttgaatgcg ttcttcttca
     3001 ctggcgcttg acgacgccgc cctgcgtcat caggcaggcg acgacgatgt cgtcgtccat
     3061 cggcacgacg agcccgcctt ccttgggcag cacgagcttg aggaagtcga gcacgttgcg
     3121 ggcatagagc gacgacgcat cggcaccgac cttcgacgcc aggttcgttt cgccgatgag
     3181 ggtcacgccg tggacgacca cggtccggtc ggcgacgctg agcgggcagt tgccgccgtg
     3241 gccgtcgggc cccttgccgg cggcgagatc gacgatcacc gagcccggct tcatgctcct
     3301 gaccatctcc tcggtgacga gcaccggcgc ggcgcggccc gggatcagcg cggtggagat
     3361 gacgacgtcg gccagcgcca cccgcttggc gacctcgacc ttctgcctgt ccagccagct
     3421 cgccggcatg gcccgcgcgt agccgccggt gccttcggca gcttcctttt cctcggccgt
     3481 ttcgtagggc acctcgatga acttcgcccc cagcgattcg acctgctcct tgacgctcgg
     3541 tcgcacgtcg ctcgcctcga tgaccgcgcc caggcgcttg gcggtggcga tcgcctgcag
     3601 gccggccaca ccgacgccga taacgacgac ccgtgccgcc ttcacggtgc ccgcggcggt
     3661 catcagcatc ggaaagaagc gctggtaggt gtcggcggcg accatcaccg ccttgtagcc
     3721 ggcgatgttg gcctggctcg acagcacgtc catgctttgc gcgcgcgtcg ttcgcggcgc
     3781 cgcctcgagc gcgaagctgg tcagcccggc ggcggcgagc gcgtcgaggc cggcgcggtc
     3841 gaacgggttg agcatgccga cgagatgggt tcccggcttc atcagcgtca gttcgccggc
     3901 gtcgggcgaa cgcaccttga ggacgagatc ggacgcgaag gtggcagcac gatcggcgat
     3961 ttcggcaccg acggcgcggt atgcgtcgtc ggtggcgctg gcggcgacgc cggcaccgct
     4021 ctcgacgcgc agcgagtgtc cctgcgccgc cagctttttc gcggtctccg gcgtgacggc
     4081 gacacgcgtt tcgccgatcg ccgtctcgcg cggcaccccg atcaacatgt tcccgtctcc
     4141 tcctgcagcg aaccccggga gcttacaaga aagggcacgc ggcccgctct gccccgatcg
     4201 gcgcggcccg tccggcacgg gacagctgat ctagcatcga cagatggacg agcgctggaa
     4261 accgagcgtc acggtcgccg cgatcgccag ccggcaggcc ggcggccggc gcgaatacct
     4321 ggtcgtggaa gaagaaaccg ccgacggcct gcgcctgaac aatcctgccg gccacctcga
     4381 gcccggcgaa tccctgcagc aggcggtggt gcgcgaggcg ctcgaggaga ccggccgctg
     4441 ctacgcgccc tcgcggctgg tcggcgtcta cctcgcccgc gtccggctcg ctggcgacgc
     4501 gcgcgacgac gcgacgtatc tgcgcttcgc gttcgccggc acggccggcg acgccgatcc
     4561 gcagcgtcgg ctcgacaagg ggatcgtcgg cacgctctgg ctgaccctgg acgaactgcg
     4621 ccgctcgcgc caccggcacc gcagcccgct ggtgctgcgc tgcgtcgaag acgacctcgc
     4681 cggccgtcac gtgccgctcg acgtcctctc caccgacctc ggcctcgccg gcggaccgcg
     4741 cacggcggcc tgagcgccga actcctcgcc gtcggccgac catggcatcg cgcctgctga
     4801 tcgccgaaga cgattacgcg atcgcgacca atctcttcac ctacttcgag cgcaagggct
     4861 acgacgtcga ggccgtgtac agcggccagg cggcgatgca tcgctgcagc gtcgagcgct
     4921 tcgatctcgt cattctcgac atcggcctgc ccgggctcga cggcctgacc ttcctgcagc
     4981 gcctgcgcgg cgaattgcgg gtggcgacgc cggtgctggt ggtgtcggcg cgcagcgacc
     5041 tctcggacaa gctggccggc ttcgagcacg gagccgacga ctacgtcacc aagcccttcg
     5101 cgctcgccga ggtcgaggcg cgcgtccgcg ccttgctcaa ccgcgcccgc ggtgccagca
     5161 tcgacccggt ccgtcgcctc ggccgcctgg ccttcgacgt ccgggaaggc gaggcgaggg
     5221 tcggcgacgc cctcgtccgc ctgaccccga aggccgccca gctcctcgac ctgctgctgc
     5281 gctacccggg acagctggtg cgtcgcgccg agatcgagaa ggccctctgg gacgacgagg
     5341 tggcgaacgc cgatgcgctg cgtagccagg tgcactggct gcgtcgcgcg ctcgccgagg
     5401 cgggcttcga cggcatcgag accgtgcacg gcgtcggcta tcggatcgtc gagcggccat
     5461 ccgcgccatg atcgcgctgt cgcgacggcg cttcacgctc acccagcgcg tcgtctgggc
     5521 ggtcacggcg acggcggcgc tgctgctcgg gctgcagagc gtgctcgcct acttcgcgat
     5581 gcatgcccag gaggacgagc tcagcgacga catgctgcgt cgcgaggtgc agcagctgat
     5641 cacgctgacg ctgaagcccg gattgatgcc cgtcggctcg ctcgccgcgt cgcccgggct
     5701 caccgcctac ctgacacgcg gatcggaggg catggcttcc gtccccgacg acgtcaggcg
     5761 actcgagccc ggcctgtacc agctggcgaa ggacggcacc gtccggcacg tcgccgtcgt
     5821 cgacaccgag gacggccggc tgcgcatcgt cctcg
//
LOCUS       DASRWS010000424         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3057996, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000424 DASRWS010000000
VERSION     DASRWS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057996"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFF72_08000"
     CDS             <1..1100
                     /locus_tag="VFF72_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZV93144.1"
                     /translation="LQQEVSQLQQEIGRVSTQTQFNGINLLDGTFTAQQFQVGANSGQ
                     TITVSSIASARPSDLGASYKATVTGSGALTALSAGSLTLNGASVQASVAGAAAGQDDN
                     SAYAIALAINASQGTVQATANAASYTGGAPTASSNLAAGDLKINGVDVGAVTGVAGAA
                     AQGAAMAAAINQVSAATGVTASADAASGAITLTAADGRNIDVSANANATTATGIANGA
                     KYGTVSLSTNSAVSVGSNIVVAGTAPGNAGLSAGTTPSALSGTAVSSLDISTVAGANA
                     ALASIDAALATIDSTRANLGAVQNRFSATIENLQTGAQNTTASRSRIQDADFAAETAN
                     LSRAQVLQQAGTAMVAQANQLPQQVLQLLKG"
     gene            1300..2787
                     /gene="fliD"
                     /locus_tag="VFF72_08005"
     CDS             1300..2787
                     /gene="fliD"
                     /locus_tag="VFF72_08005"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZV93145.1"
                     /translation="MTTISPTQGTISSAGIGSGLDVNSIISSLMAVESLPLTALQTKA
                     TGLQTDLSAFGQVQSLVSALQDAAKPLYDPNTFKLTNTSSADPSSVSAVTSGSAVPGN
                     YTLSVSSLSAAQSVVSPVGQFTDGTSVVGTGSLTIRLGSWSSDNSSFTPATGSSDITI
                     PIGASENTLAGIRDKINAANAGVSATIVTDASGARLAIQSSSTGSANGFRISVADDDG
                     NNADASGLSRLAYDPASSNAQMTLAQAAANTQATINGIAVTTASNSLSGVIDGITFNL
                     GKTTTQPVTVTVTQNTDAIKADMTALVSAFNALNTFMAAETKYDPTTNTAAPLQGDST
                     AVSIQNQMHSLLAQTTGASSIFSTLTSMGVRLQTDGSLTLDDATWNAAAANLPELTKA
                     LSNVDATNSQNNGFGKRFSDWTTQLLQTGGTLTGKTAAIQASITQNQKDQTTMNTQLA
                     ATQARLQAQYTALDSTMSQANALAAYVQQQFYAKSSFGTNSSNSN"
     gene            3009..3446
                     /gene="fliS"
                     /locus_tag="VFF72_08010"
     CDS             3009..3446
                     /gene="fliS"
                     /locus_tag="VFF72_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZV93146.1"
                     /translation="MFSNTFQPGQRPTVLGLYRNVGAASGVEGASPHKLVCMLYEAVC
                     GEIAAARGALARRDVAEKGRAIGKAVRLIEEGLLAPLDLEAGGAIAANLRDLYQYMVY
                     RLTMGNLHSDDAALADCAGLARTLSDTWTTIAPQVDAPARAAA"
     gene            3482..3781
                     /locus_tag="VFF72_08015"
     CDS             3482..3781
                     /locus_tag="VFF72_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HZV93147.1"
                     /translation="MNSVLMNYYEAIEKASQDMLEAARVGNWDHVVKLEGACALLIAQ
                     LKNTAAEKPLHADEAQLKSRIMQRILVNDAEIRQLAEPWLDDLDEILAGRPKTLH"
     gene            3871..>4343
                     /locus_tag="VFF72_08020"
     CDS             3871..>4343
                     /locus_tag="VFF72_08020"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HZV93148.1"
                     /translation="METRPAPLDGMNDNEGLDEFRVTSPREIQGLLKQLLDGSVLLNL
                     NASDGSVVTSAIWTLDSSRGTVGFNADPNDPGIQSLLECEEAMVVGYLDSVKLQFEVH
                     DLVLVHGHRASVLSCPAPREMYRFQRRNAFRVRPLMRSAPVARLRHPDMPEMEFAL"
BASE COUNT          812 a         1576 c         1374 g          581 t
ORIGIN      
        1 cgctgcagca ggaagtcagc cagcttcagc aggaaatcgg tcgtgtctcg acgcagacgc
       61 agttcaacgg catcaacctg ctcgacggca cgttcacggc acagcagttc caggtcggag
      121 caaattccgg ccagacgatc accgtctcct cgatcgccag cgcaagacct tccgacctcg
      181 gtgccagcta caaggccacc gtcacaggtt cgggcgcatt gaccgcgctc agcgccggct
      241 ccctgacgct caacggcgcg agcgtgcagg cgtcagtcgc cggcgcagcc gcgggccagg
      301 acgacaacag tgcctacgcg atcgcgctgg ccatcaacgc gtcgcaaggc accgtccagg
      361 cgacggcgaa tgcggcctcc tataccggcg gtgcgccgac cgcgtcgagc aatctggcag
      421 cgggcgacct gaagatcaac ggtgtcgacg tcggtgcggt gaccggtgtc gccggcgccg
      481 cggcacaagg cgcggcgatg gcagcggcga tcaaccaggt gtcggcggcc accggcgtca
      541 ccgccagcgc cgacgcagcc tccggggcta tcacgctgac ggcagcggac ggacgaaaca
      601 tcgacgtcag cgccaacgcc aacgcgacca ccgcgacagg catcgccaat ggcgcgaagt
      661 acggcaccgt ctcgctgtcc acgaactccg ccgtctccgt cggaagcaac attgtcgtgg
      721 ccgggaccgc gccggggaac gccggcttga gcgcaggtac gaccccttcg gcgttgtccg
      781 gcaccgcggt ttcatcgctc gacatctcga ccgtcgcagg cgcgaacgcc gccctggcgt
      841 cgatcgacgc ggcgctggcg accatcgatt cgacgcgcgc caacctgggt gccgtccaga
      901 accgcttctc ggcgacgata gagaacctgc aaacgggcgc ccagaacacg acggcatcgc
      961 gcagccgcat ccaggacgcc gacttcgccg cggaaacggc gaatctgtcg cgtgcccagg
     1021 tgctgcagca agccggcacg gccatggtgg cgcaggccaa tcagttgccc caacaggtgc
     1081 tgcaactcct caagggctga tcgagacaca agcgggcatc ttgtcggtcc gtgcaagcga
     1141 ccgctcaaga tcgactggcg cccgccgata acccagccat ggcgacgcag cggctcgccg
     1201 ctttccgacg gcgcccgatg atgaaacacc cgatcccgca cttgcccgct gtctccgacc
     1261 ggagccgtca gaggcttgcg gcgggcgggc gctgctgaca tgaccaccat ctcgcccacg
     1321 cagggcacga tctcgtcggc cggcatcggc agcggcctcg acgtcaactc gatcatcagc
     1381 tcgctgatgg cggtcgagag cctgccgctg acggcgctgc agaccaaggc gaccggcctg
     1441 cagaccgacc tgtcggcgtt cggccaggtc cagtcgctgg tctcggcgct gcaggacgcg
     1501 gccaagccgc tgtacgaccc gaacaccttc aagctcacca acaccagctc tgctgatccg
     1561 tcgagcgtca gcgccgtcac ttcgggcagt gccgtgccgg gaaactacac cttgtccgtg
     1621 tcgtcgcttt cggcggcgca gtcggtcgtc agcccggtcg gccagttcac cgacggcacg
     1681 agcgtcgtcg gcaccggcag cctgacgatc cgtctgggca gctggagcag cgacaactcg
     1741 agcttcacgc cggcgacggg atcgtccgac atcacgatcc cgatcggtgc cagcgaaaac
     1801 acgctggccg gcatccgcga caagatcaac gccgccaacg ccggcgtcag cgcgaccatc
     1861 gtcaccgacg caagcggcgc gcgactggcg atccagtcga gctccaccgg cagcgcgaac
     1921 ggatttcgca tcagcgtcgc cgacgacgac ggcaacaacg ccgatgcgtc cggcctgtcg
     1981 cgcctggcct acgacccggc gtcgtcgaat gcgcagatga cgctggcgca ggcggccgcc
     2041 aacacccagg cgacgatcaa cggcatcgcc gtcacgaccg cgtcgaactc gctgtcgggc
     2101 gtgatcgacg ggatcacctt caacctgggc aagacgacga cccagccggt caccgtcacc
     2161 gtgacgcaga acaccgacgc catcaaggcc gacatgacgg cgctcgtttc cgccttcaat
     2221 gcgctcaaca cgttcatggc ggccgagacg aagtacgacc cgacgaccaa cacggcggcg
     2281 ccgctgcagg gcgacagcac cgcggtcagc atccagaacc agatgcactc gctgctcgcg
     2341 cagacgaccg gcgcgtcgtc gatcttctcg acgctcacct cgatgggcgt ccggctgcag
     2401 accgacggat cgctgacgct cgacgacgcc acctggaacg ccgccgcggc caacctgccg
     2461 gagctgacca aggccctttc caatgtcgac gcgacgaact cgcagaacaa cggcttcggc
     2521 aagcgcttct ccgactggac gacacagctg ctgcagaccg gcggcacgct gaccggcaag
     2581 acggcggcga tccaggcgag catcacccag aaccagaaag accagacgac gatgaacacg
     2641 cagctggcgg cgacccaggc ccggctgcag gcgcaataca ccgcgctcga ctcgacgatg
     2701 tcgcaggcca acgccttggc cgcctacgtc cagcagcagt tctacgccaa gagcagcttc
     2761 ggcacgaaca gctcgaactc gaactgacgc gtcgagccgg cccggccacg cgggttcgag
     2821 cgggagggga aagcgttgtc gacccggccg gtcgtcatcg gcgcggcagt tcgcgccttc
     2881 ggcgaagccg ggccgccggc gctcgtttcc gatccatacg attgccctgc caaaacgcgc
     2941 caagcgcgcg gtaaagattc gctccccggc gtcgataaac atggcagaga cgaataggtg
     3001 atcgccacat gttcagcaac acattccagc ccggccagcg gccgaccgtg ctcggcctgt
     3061 acaggaatgt cggcgccgcg agcggcgtcg agggcgccag cccgcacaag ctggtctgca
     3121 tgctgtacga agccgtgtgc ggcgagatcg ccgcggcccg cggtgcgctg gcccggcgcg
     3181 acgtcgccga gaagggccgc gcgatcggca aggccgtgcg cctgatcgag gagggcttgc
     3241 tggccccgct cgacctcgag gcgggcggtg cgatcgccgc caatcttcgc gatctctacc
     3301 agtacatggt ctaccggctg accatgggca acctccattc ggacgacgcg gcgcttgccg
     3361 actgcgcggg cctggcccgc accctcagcg acacctggac gaccatcgcg ccgcaggtcg
     3421 acgccccggc acgggcggcg gcatgacgcg gccccaggca ctttcaccgg aacagacgat
     3481 catgaattcc gtgctgatga actactacga agcgatcgag aaggcgagcc aggacatgct
     3541 cgaggccgcc cgcgtcggca actgggacca cgtcgtcaag ctcgagggcg cctgcgccct
     3601 gctgatcgcc cagctgaaga acaccgccgc cgagaaaccc ctgcacgccg acgaggcgca
     3661 gctgaagtcg cggatcatgc agcgcatcct cgtcaacgac gccgagatcc gccagctcgc
     3721 cgagccctgg ctcgacgacc tcgacgagat cctcgccggc cgtcccaaga ctctgcactg
     3781 agctttcgcc gcgcccgccg ccagcccgca cctccgtccg accctcgcga cgccagccgt
     3841 cgccgaccac gcagcgccac gaggcacgcc atggaaacca gaccggctcc tctcgacggg
     3901 atgaacgaca acgaggggct cgacgagttc cgcgtcacgt cgccgcgcga gatccagggc
     3961 ctgctcaagc aattgctcga cggctcggtg ctgctcaacc tcaacgccag cgacggcagc
     4021 gtcgtcacga gcgcgatctg gacgctcgac agcagccgcg gcaccgtcgg cttcaacgcc
     4081 gacccgaacg atccggggat ccagtcgctg ctcgaatgcg aggaggcgat ggtcgtcggc
     4141 tacctcgaca gcgtcaagct gcaattcgag gtgcacgacc tggtgctggt gcacggccat
     4201 cgggccagcg tcctcagctg cccggcgccg cgggagatgt accgcttcca gcgccgcaac
     4261 gcctttcgcg tcaggccgct gatgcgcagt gcgccggtgg cgcgcctgcg ccacccggac
     4321 atgccggaga tggagttcgc gct
//
LOCUS       DASRWS010000425         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_176604, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000425 DASRWS010000000
VERSION     DASRWS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176604"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFF72_08025"
     CDS             <1..292
                     /locus_tag="VFF72_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZV93149.1"
                     /translation="VGFFALIGMRVLPALNEYFTIKRAVNRVATEGSTVPEIRAAFER
                     QKDIEYSISSISGKDLNITKDNDKIVVSFAYDKEIELMKPVYLLIKFEGRSN"
     gene            349..1053
                     /gene="rnc"
                     /locus_tag="VFF72_08030"
     CDS             349..1053
                     /gene="rnc"
                     /locus_tag="VFF72_08030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZV93150.1"
                     /translation="MDSSLVALQQRTGHHFHDQALLQRALTHRSFGTDHNERLEFLGD
                     AVLSLVVSSLLFDRLGHLDEGDLTRVRAHLVREDSLHRAALQLGLPELLRLGEGEARG
                     GGALRASILADALEALIGATFQDGGFDAARALVQSLFGEIIETTDISSWSKDAKTELQ
                     EWLQARRLPVPSYRIVATRGQAHAQTFEVECMVPALGLRETGEGRSRRAAEQEAARRM
                     IDALSASDQPGSPLRS"
     gene            1053..1982
                     /gene="era"
                     /locus_tag="VFF72_08035"
     CDS             1053..1982
                     /gene="era"
                     /locus_tag="VFF72_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZV93151.1"
                     /translation="MGDSPAPAGTPGAQRCGVVAIVGRPNVGKSTLLNALVGQKVSIT
                     SNKAQTTRHRITGIRTLGDAQFVFVDTPGFQTRHGAPLHRTLNRAVTGALSEVDVVLF
                     VVEAGRFGKADASVLDLVSADRPVILIANKLDTVKRRADLAPWLLEMQGRESKGRHGF
                     AEIVPLAATRPADAARLLQIVSPYLPIRPWLHDEDALTDRNERFLAAEIVREKLFRLT
                     GDELPYTSTVVIDRYEEEGNLHRIAASIVVERDAHKAMVIGSGGERLKRIGTEARHEL
                     EHLLDAKVFLELWVTVKSGWADDEAHLRSYGYE"
     gene            1991..>2570
                     /gene="recO"
                     /locus_tag="VFF72_08040"
     CDS             1991..>2570
                     /gene="recO"
                     /locus_tag="VFF72_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZV93152.1"
                     /translation="MASRSSRSPALVAFVLHRYDWSESSLILDLFTRERGRLAAAAKG
                     ARRPFSQLRSVLLPFQRIQVSVGSLPADENARDIENLRSAEWMGGGAMLTGAALFNGF
                     YLNELLMRLLARHDPHPALFDAYSATIASLGTDDAGSQAALRGFEMVLLKEIGVLPDL
                     AIETSTGRPVAAEERYRVLADSGVVAAAGATDS"
BASE COUNT          423 a          885 c          843 g          419 t
ORIGIN      
        1 cgtcggcttt ttcgcgctca tcgggatgcg cgtgctgccg gccctgaacg aatacttcac
       61 gatcaagcgg gcggtcaaca gggtcgccac agaaggctcc acggtgcccg agatccgcgc
      121 cgccttcgag cgtcagaagg acatcgaata ctcgatctcc tcgatttccg gcaaggacct
      181 gaacatcacc aaggacaacg acaagatcgt ggtcagcttc gcctatgaca aggagatcga
      241 gctgatgaag ccggtctacc tgctcatcaa gttcgaaggc cgatcgaact aggatcgggg
      301 tttggcgctc gaaccaggcc cgccgcccgt ggcccgccgt ctcttcgcat ggattccagt
      361 cttgtcgccc ttcaacagcg caccggccac catttccacg accaggcgct tcttcagcgc
      421 gcgctgacgc atcgcagctt cggcaccgat cacaacgagc gcctcgagtt cctgggcgac
      481 gccgtgctga gcttggtcgt gtcgagcctg ctcttcgatc gcctgggcca tctggacgaa
      541 ggcgatctga cgcgcgtgag agcgcacctc gtccgcgagg acagcctgca ccgggccgcg
      601 ctgcagctcg gcctgccaga actccttcgc ctgggcgagg gtgaggccag gggcggcggc
      661 gcgctgcgcg cgtcgatcct ggccgatgcc ctggaggcgc tgatcggcgc gacgttccag
      721 gatggcggct tcgacgcggc ccgcgcgctc gtgcagagcc tgtttggcga gatcatcgaa
      781 acgaccgaca tctcgagctg gagcaaggat gccaagacgg agttgcagga atggctgcaa
      841 gcgcgtcgcc tgccggtacc gagctaccgg atcgtcgcga cgcgcggcca ggctcatgcg
      901 cagacgttcg aagtcgagtg catggtgcct gccctcggat tgcgcgagac cggggaaggc
      961 aggtcgcgtc gcgccgccga gcaggaagcg gcgcggcgga tgatcgacgc gctgagcgcc
     1021 agcgatcagc cgggcagccc gcttcgctcc tgatgggcga ctcgccggcg ccagcgggca
     1081 cccccggggc gcagcgctgc ggcgtggtcg ccatcgtcgg ccgccccaac gtcggcaagt
     1141 cgaccctgct caacgcgctg gtcggtcaga aggtcagcat cacgtcgaac aaggcgcaga
     1201 cgacgcgcca tcgcatcacc ggcatcagga cgctcggcga tgcgcagttc gtcttcgtcg
     1261 acaccccggg attccagacc cggcacggcg cgccgctgca ccgcaccctc aaccgggccg
     1321 tcaccggtgc gctgtccgag gtggacgtgg tgctcttcgt cgtcgaggcg ggtcgcttcg
     1381 gcaaggccga tgcctcggtc ctcgatctgg tgtccgccga ccgtccggtc atcctgatcg
     1441 ccaacaagct cgacacggtg aagcgccgcg ccgatctcgc accgtggctg ctcgaaatgc
     1501 aaggtcgcga gtcgaagggc cgtcatggct tcgccgagat cgtcccgctt gcggcgacac
     1561 gtccggccga tgcggcgcgg ctgctccaga tcgtttcgcc ctacctgccc atccggccgt
     1621 ggctgcacga cgaagatgcg ctgaccgatc gaaacgaacg cttcctcgcc gccgagatcg
     1681 tccgcgaaaa gttgttccgg ctgaccggcg acgagctgcc ctacacctcc accgtcgtca
     1741 tcgaccgcta cgaggaggaa ggcaacctgc atcgcatcgc cgcctcgatc gtcgtcgaac
     1801 gcgacgcgca caaggcgatg gtcatcggca gcggtggcga gcggctcaag cggatcggca
     1861 ccgaagcgcg gcacgagctc gaacacctgc tcgatgccaa ggtgtttctc gagctgtggg
     1921 tgacggtgaa gagcggctgg gccgacgacg aggcccactt gcgctcctac ggctacgaat
     1981 agcatcgggc gtggcttcac gatcctcccg cagcccggca ctcgtcgcct tcgtcctgca
     2041 ccgctacgac tggagcgaat cgagcctcat cctcgatctg ttcacgcgcg agcgcggccg
     2101 gctggccgcc gccgctaagg gtgcgaggcg gccgttctcg caactgcgca gcgtgctgct
     2161 gccgttccag cgcatccagg tttccgtcgg cagcttgccg gcggacgaga acgcgcgaga
     2221 catcgagaac ctgcgcagcg ccgagtggat gggcggcggc gccatgctga ccggcgcggc
     2281 gctcttcaac ggcttttacc tgaacgagct gttgatgcga ctgctggctc gccatgaccc
     2341 gcacccggcc ctgttcgacg cctattcggc gacgatcgcc tccctcggca ccgacgatgc
     2401 cggcagccag gcggcgctgc gcggattcga gatggtgctg ctgaaggaga tcggcgtcct
     2461 gcccgacctg gccatcgaga cgtcgaccgg ccggcctgtc gctgccgaag agcgttaccg
     2521 cgtcctcgcc gattccggcg tcgtcgccgc tgcaggggcc accgattcga
//
LOCUS       DASRWS010000426         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2194982, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000426 DASRWS010000000
VERSION     DASRWS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194982"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1464
                     /locus_tag="VFF72_08045"
     CDS             244..1464
                     /locus_tag="VFF72_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93153.1"
                     /translation="MILFRLRLPIAAATLSAVVLSFAAHAAPDPCAGIEHCQNLGPFT
                     ATVLKVNVTRQDTVTAYQGVRTTVRFTNIGRQPLVLAYRDHSSLVTDNNGLAYKRSAR
                     AYGIGVVTRDSADPQFELAPGESREASFDGTLQYSMRRQVAGNVFTHDIGIVQLAVEG
                     DRRVREVRDYAVSFAGLTSTSGYGGAPAPGGASPVTASPQGAAGPVAGSGAGGDACAG
                     QPACQAQGPFTASVVRVTVTDSGRVTANHVVRTTLRFRNVSDRPIALGYRRNSNRVSD
                     NQGHTYTRFTSTGGDAAHVFGIGLVTDGSADPQFTLAPGESRDASFEAALQYNVRYTQ
                     PGTVFSQDMTIAELEIVGPRQVRTAHDYALTFSNLTATTGGGLAAGTGPAGGPAPNAD
                     AVNKVVDLFKALRR"
     gene            1535..2521
                     /locus_tag="VFF72_08050"
     CDS             1535..2521
                     /locus_tag="VFF72_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93154.1"
                     /translation="MHAINATIVLAIAAGLAGCGKTPAPAASGNPAPLVATARPATTG
                     NASAEEVASQARGSVSCPASIATPAHSASAPVDDIVGVRPGLTYEEAGNVVECSNELL
                     VVSAETSRGFDIKSYGQKIRQGFSARFAEPRVVKTSKQIMKEMQDDAMARGMNARRED
                     LKPGQVKWYVSTMGLPGQERVLAAAREERFVEAQSPTLASVGEALLKKYGTPTREQGP
                     QGQAKSMRWAYDPLGRRITETSPLYARCVGAASPDAGLHLSRDCGVIVEAMLIPQRAN
                     PDLVDRMQVGVVDQAGGDRMVAATELGLQQMDGQRRAQEVAKAGKNARAPSL"
     gene            2533..3297
                     /locus_tag="VFF72_08055"
     CDS             2533..3297
                     /locus_tag="VFF72_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93155.1"
                     /translation="MIANSFRLIGVATALALAGCGPTTSGASSGAGGQALATKTAGAG
                     PTALPKSGSTPTVAGTAVAAPAVASGREVVNPDKSTMVFLYLALAGLAPPIDDWVEDD
                     ARVRAAAPIDKAARRSAVRAELESGVAAARGIGRIRVTLADAGVSDYDPTYQEFTVRS
                     LSPGSELDYSAFGQRVKTRFDNGETAQLWHVPSGQAQAVRDRLSAGRGVELDVLLRVT
                     GAQPGTGGGTITTEIVDYTLRSRDGSKLGRVQVAKE"
     gene            complement(3445..>3587)
                     /locus_tag="VFF72_08060"
     CDS             complement(3445..>3587)
                     /locus_tag="VFF72_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZV93156.1"
                     /translation="GIQVALHVHVPGMDKTQAKKLVDAAHQVCPYSNATRGNIEVTLD
                     VF"
BASE COUNT          584 a         1243 c         1270 g          490 t
ORIGIN      
        1 agcggcaagc gctcgccgcg cgtgttgacg aggaccggca ggtcgagcaa ctcgatccgc
       61 gcggtggcag cgacggtatg cgcggacggg tgggccatgc ggcaaatcct aaacggctcg
      121 ccggaccgtc gcgaataacg cgccggtaac gccttggcgc aagagtgcgg cacgggtcga
      181 ccgcatctcg cggcgactcc ggaaggcgcc cgtcgcgtcg cgtctcgttc gaggagctgc
      241 tccatgatcc tgttccgcct tcgcctgccc atcgccgcag cgacgctgtc cgccgtcgtc
      301 ctgtcgttcg cggcgcatgc cgcacctgat ccctgcgcgg gcatcgagca ctgccagaac
      361 ctcggtccct tcaccgcgac agtgctgaag gtcaacgtga cgcggcagga caccgtcacc
      421 gcctaccagg gcgtgcgcac cacggttcgc ttcacgaaca tcggtcgtca gccgctggtg
      481 ctggcctatc gggatcattc gagcctggtg accgacaaca acggcctggc ctacaagcgg
      541 tcggcgaggg cctacggcat cggcgtcgtg acgcgcgact cggccgaccc gcagttcgag
      601 ctggcgccgg gcgagtcgcg cgaggcatcg ttcgacggca cgctgcagta cagcatgcgc
      661 cggcaggtgg cgggcaacgt gttcacgcac gacatcggca tcgtccagct cgcggtcgag
      721 ggcgatcgac gtgtccgcga agtgcgcgac tacgcggtga gcttcgcggg attgacgtcg
      781 acctcgggct atggcggtgc gccggcgccc ggcggcgcat caccggtcac ggcatcgccg
      841 caaggcgctg ctggcccggt cgcgggcagc ggcgccggcg gcgacgcctg cgccggccag
      901 ccggcctgcc aggcgcaagg cccgttcacg gccagcgtcg tgcgtgtcac cgtcaccgac
      961 agcggcaggg tcaccgccaa tcacgtcgtc cgcacgacgc ttcgcttcag gaacgtgagc
     1021 gatcgcccga tcgcgctcgg ctatcggcgc aacagcaacc gcgtcagcga caaccaggga
     1081 cacacctaca cgcgcttcac gtcgaccggt ggcgacgcgg cgcacgtctt cggcatcggc
     1141 ctggtgacgg acggctcggc cgatccgcag ttcacgctcg ccccgggcga gtcgcgcgac
     1201 gcgtcgttcg aagcggcatt gcaatacaac gttcgataca cgcagcccgg caccgtgttc
     1261 tcgcaggaca tgaccatcgc cgagctggag atcgtcggcc cgcgccaggt ccgtacggcg
     1321 cacgactacg ccctgacgtt ctcgaacctg accgcgacga ccggcggcgg cctggcggcc
     1381 ggcacgggcc cagccggcgg tcctgcgccg aacgccgacg cggtgaacaa ggtggtggac
     1441 ctgttcaagg cacttcgcag gtaggcgaac ccgcgcggcc gtgccgccgc gatcggcgac
     1501 gacgcaccca gcaacatcga ggaggacttc gaccatgcat gcaatcaacg cgaccatcgt
     1561 tctcgccatt gccgccggac tggccggttg cggaaagaca cccgctcccg ccgcatcggg
     1621 aaatccggcg ccgctcgttg cgacggcccg gccggcgacg accggcaatg ccagcgccga
     1681 ggaagtggcc agccaggcgc gaggcagcgt gtcttgcccg gcatcgatcg cgacgccggc
     1741 gcattccgcg agcgcgccgg tcgacgacat cgtcggcgtc cgacccggcc tgacctacga
     1801 ggaggccggc aacgtcgtcg aatgcagcaa cgagctgctc gtcgtttcgg ccgagacgag
     1861 ccgcggcttc gacatcaaga gctacgggca gaagatccgc cagggcttca gcgcccggtt
     1921 cgccgagccg cgcgtcgtca agaccagcaa gcagatcatg aaggagatgc aggacgacgc
     1981 catggcgcgc ggcatgaacg cgaggcgcga agacctcaag cctggccagg tgaagtggta
     2041 cgtgagcacg atgggcctgc cgggccagga gcgggtgctt gccgcggcac gcgaggagcg
     2101 attcgtcgag gcgcagagtc cgacgctggc gagcgtgggc gaggccttgc tcaagaagta
     2161 cggaacgccg acccgcgagc aggggccgca gggccaggcg aagtcgatgc gctgggccta
     2221 cgacccgctg gggcgccgga tcaccgaaac ctcgccgctc tatgcgcgtt gcgtcggcgc
     2281 ggccagtccc gacgccggct tgcatctgtc gcgcgattgc ggcgtgatcg tcgaggcgat
     2341 gctcattccg cagcgcgcca accccgacct ggtcgacagg atgcaggtgg gcgtcgtcga
     2401 ccaggccggc ggcgaccgga tggtcgcagc gaccgagctg ggcctgcaac agatggacgg
     2461 acaacggcgg gcgcaggagg tggccaaggc ggggaagaac gccagggcgc cgtcgctgta
     2521 ggagagggag cgatgatcgc gaattcgttc cgcctcatcg gtgtcgccac ggccttggca
     2581 ctcgccggct gcggccccac gacgtctggc gcgtcgtccg gggcaggcgg tcaggcactc
     2641 gccacgaaga ccgcgggcgc cggccccact gcgctgccga aatcggggtc gacgccgacg
     2701 gttgccggca ctgcggtcgc ggcgccggcg gtcgcttccg gccgcgaagt cgtcaacccc
     2761 gacaagtcga cgatggtgtt tctctaccta gcgctggccg gtcttgctcc gccgatcgat
     2821 gactgggtcg aggacgacgc ccgggttcgc gcggcggcgc cgatcgacaa ggcggcgcgt
     2881 cgcagcgcgg tccgcgccga gctcgagtcg ggtgtcgctg ccgcccgcgg gatcgggaga
     2941 atccgcgtca cgctggccga tgccggcgtc agcgactacg acccgacgta ccaggagttc
     3001 accgtgcgct cgctgagccc cggctcggag ctcgactaca gcgcgttcgg ccagcgcgtc
     3061 aagacccgat tcgacaacgg cgaaaccgcg cagctctggc atgtgccgag cggccaggcg
     3121 caagcggtcc gggacaggct gtcggccgga cgtggggtcg agctcgacgt cctgctccgg
     3181 gtcaccggtg cgcaacccgg aaccggcggc ggcacgatca ccacggagat cgtcgactac
     3241 acgctgcgct cgcgagacgg cagcaagctt ggacgcgtgc aggtcgccaa ggagtgaggc
     3301 ggcgcaggct cgcgcggcac ttcgagacgg tcggtcggcg cggtccgctg ccccgggttc
     3361 ggcacgtcga gcccctcggc agtgccgggg cacgtcgccg gaaccgacgc cgaccggcac
     3421 ccgcgcaagc gacgaatccc aggactagaa gacgtcgaga gtcacctcga tgttgccgcg
     3481 cgtcgcgttg gaataggggc acacctgatg ggccgcgtcg acgagctttt tcgcctgcgt
     3541 cttgtccatg cccggcacgt gcacgtgcag cgccacctgg atgccga
//
LOCUS       DASRWS010000427         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1619101, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000427 DASRWS010000000
VERSION     DASRWS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619101"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /gene="rpsI"
                     /locus_tag="VFF72_08065"
     CDS             complement(<1..141)
                     /gene="rpsI"
                     /locus_tag="VFF72_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZV93157.1"
                     /translation="MIGNWNYGTGRRKSSVARVFMKKGTGQILVNGKPVEEYFGRQTS
                     IMV"
     gene            complement(138..578)
                     /gene="rplM"
                     /locus_tag="VFF72_08070"
     CDS             complement(138..578)
                     /gene="rplM"
                     /locus_tag="VFF72_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZV93158.1"
                     /translation="MKTFSAKAAEVTHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDFIVIVNADKIRVTGNKADDKIYYRHSGYPGGIRGMSFRDMQARHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYAGDTHPHTAQQPKTLTLQDAQ"
     gene            837..1565
                     /locus_tag="VFF72_08075"
     CDS             837..1565
                     /locus_tag="VFF72_08075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV93159.1"
                     /translation="MQDIDTLPEAKASLAPADPGNGGTSAVAGNGSPDGTSTRRDWVP
                     IRSLATRHRERILKHLLALDERSRYLRFGYAATDAHISRYVDAIDFEHDEVFGIFNRR
                     LELIALAHLAFRPGGATDSHGPAGEFGVSVLTKARRRGFGRRLFEHAVLHARNRGVQA
                     MFIHALSENTAMLRIARDAGAEIVREGSESEAWLKLAPETLGSHIDELVGERLAEFDY
                     RLKHQAHHLKNMFKRSDDPGKGDG"
     gene            1680..2477
                     /locus_tag="VFF72_08080"
     CDS             1680..2477
                     /locus_tag="VFF72_08080"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HZV93160.1"
                     /translation="MAGLLLLVIVTLWLARRAPRVEPLPTEWALSARPVFSTDERRVH
                     KLLREALPHHVVLSKLPLVRFCQPNDPGEVRFWYDLLGQINVTFAVCSANGRVLAAID
                     LDTDRGNSRRILQIKQSVLGACRIRYLRCPVDNLPTAAELQLLVPQGAGATRAPQPGS
                     THGLDEARDSLASTVANRRAQRTALWQDSSLFQDSFFAPDSRLDGFGQSEFSGLTQNV
                     GGPVGVGRFDHEAVNGRAPAAPRGLSGEDIAGVVVDTAPATASHSRH"
     gene            2494..3543
                     /locus_tag="VFF72_08085"
     CDS             2494..3543
                     /locus_tag="VFF72_08085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZV93161.1"
                     /translation="MSAVGDGPEALAAPYAELTPAAVLDALDGVGLRGDGRMLQLNSY
                     ENRVFQAFLEDGRVVVAKFYRPGRWSDAQIVEEHAFAAELAAAEIPVAVPLVLGRDPR
                     SMYAASVTPVSATLATTTIGGHAYRFGVCERLAGRAPELGQPDVLRWLGRFIGRIHAV
                     GAKARFAERLTLDVASYGESSRDWLLENDSVTPDTSAVWRDLVDTALTACREAFESVP
                     GRTLRLHGDCHPGNVLWTDAGPHFVDLDDAVNGPAIQDLWMLLSGDGPAMSQQLLDIL
                     EGYESFMEFDWRERRLVEPLRTLRMIHHSAWIAKRWSDPAFPIAFPWFASPAYWADQC
                     TRLREQLAAMEDAPP"
     gene            complement(3618..>3915)
                     /locus_tag="VFF72_08090"
     CDS             complement(3618..>3915)
                     /locus_tag="VFF72_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426972.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZV93162.1"
                     /translation="KDVRVTTRLVDSPACLVAEEGDMSGHLARLLKQAGQGAPKAEPI
                     LEINPEHALVRRLESSTHFDDLANILFDQALLAEGGQLEDPASYVRRVNSLLAA"
BASE COUNT          584 a         1371 c         1287 g          673 t
ORIGIN      
        1 caccatgatc gaggtctggc ggccgaagta ttcctcgacc ggcttgccgt tgacgagaat
       61 ctgtcccgtg cccttcttca tgaagacgcg cgccaccgac gactttcgac ggccggttcc
      121 gtagttccag tttccgatca ttgcgcgtcc tgcagggtga gggtcttggg ttgctgcgcc
      181 gtgtgcggat gcgtgtcgcc cgcgtagacc ttcagcttct tgatcatcgc gtagccgagg
      241 ggccccttgg ggagcattcc cttgacggcc ttctcgagcg cccgcccggg atgccgggcc
      301 tgcatgtcgc gaaagctcat gccgcggatg ccaccgggat acccggagtg tcgatagtag
      361 atcttgtcgt cggccttgtt gccggtgacg cggatcttgt cggcattgac gatgacgatg
      421 aaatcgccgg tatcgacgtg aggcgtgtag atggctttgt gcttgccgcg caggcggagg
      481 gcgacttcac tggccaccct gcccaggacc ttgtcggtcg cgtcgatcac gaaccactca
      541 tgcgtcactt cggcggcttt ggcgctgaag gttttcatgg aagattgctg ttgttgccca
      601 cggggcgaga cctgccttcg ttcggacccg cttcgatcgg cggacgctca ctcgagaccg
      661 gtcatccctt gcgcagatgc gcacgtttcg ctcgggcgaa atgcgaaaag cctgtgagta
      721 tagcagccgc gtgtccgacg ccgattcggg gcgggcagga cgctgcgcac gatcggcaaa
      781 cgctctagac tcgggtaaac cctagtcgac cagctcagcc cggccgagat ccccccatgc
      841 aagacatcga caccctgccc gaggcgaagg cttcgctcgc ccctgccgac ccgggcaacg
      901 gaggcacctc ggccgtcgcc ggaaacggca gcccggatgg cacctcgaca cggcgcgact
      961 gggttccgat ccggtccctg gcgacgcggc atcgcgagcg gatcctcaag cacctgcttg
     1021 ccctcgacga gcgctcgcgc tatctgcgat tcggctacgc cgccaccgat gcgcacatct
     1081 cccgctacgt ggacgccatc gacttcgagc acgacgaggt tttcggcatc ttcaaccgcc
     1141 gcctcgaatt gatcgcgctc gcccacctcg cctttcgccc gggcggtgcc accgacagcc
     1201 atggcccggc cggcgaattc ggcgtttcgg tgctgaccaa ggcccgtcgg cgcggcttcg
     1261 gccggcgcct cttcgagcat gcggtgctcc acgcccgcaa tcgaggcgtg caggcgatgt
     1321 tcatccatgc gttgagcgaa aacacggcca tgctgcggat cgcgcgcgac gccggcgccg
     1381 agatcgtgcg cgaaggctcg gagagcgaag cgtggctgaa gctggctccg gagacgctgg
     1441 gctcgcacat cgacgagctc gtcggcgaac ggctcgccga gttcgactat cggctcaaac
     1501 accaggccca tcatctcaag aacatgttca agcggtcgga cgacccggga aagggggatg
     1561 gctgaggaag ggcggccccg ggtaacatgc ctcgcttcgc gtcccggtca tcatcgggca
     1621 ggtcggtgtc cgagagatag ggcagcaggg ttatggcgac gcttccgtgg atcgcagcat
     1681 tggcagggct cctgctgctc gtcatcgtca ccctctggct ggcgcgccgt gcgcctcgtg
     1741 tcgaaccgtt gccgaccgaa tgggcgctgt ccgcccggcc ggtgttctcc accgacgagc
     1801 gccgcgtcca caagctgctc cgcgaggcgc tgccgcatca cgtggttctg tccaagctgc
     1861 cgctggttcg cttctgccag ccgaacgacc ccggcgaagt gcgcttctgg tacgaccttc
     1921 tcggccagat caacgtcacg ttcgccgtct gcagcgccaa cggccgggtt ctcgccgcca
     1981 tcgacctcga caccgatcgc ggcaattcgc gccggatcct gcagatcaag cagtcggtgc
     2041 tcggggcctg ccggattcgc tacctgcgct gccccgtcga caaccttcct accgcggccg
     2101 agctgcagtt gctcgtgccg cagggcgccg gtgcgacacg tgcgccccag ccggggtcga
     2161 cgcacggcct ggacgaagcc cgcgactcgc tggccagcac ggtcgcgaat cgccgcgcgc
     2221 agcgcacggc gctctggcag gactcgtcgc tgttccagga ctccttcttt gcgcccgaca
     2281 gccgtctcga cggcttcggc cagagcgagt tcagcggcct gacccagaac gtcggagggc
     2341 ctgtcggcgt cggccgcttc gaccacgaag ccgtcaatgg ccgcgccccc gccgcgccgc
     2401 gtggcctgag cggcgaggac atcgccggcg tcgtcgtcga cacggcgccc gccaccgctt
     2461 cgcattcgcg tcattgacgc cgccggaccg agcgtgagcg ccgtcggcga cggtcccgaa
     2521 gccctcgccg ccccctatgc cgagctgacg ccggctgccg ttctcgacgc gctcgacggc
     2581 gtcggcctgc gcggcgacgg ccggatgctg cagctcaatt cgtacgagaa ccgcgtcttc
     2641 caggccttcc tcgaagatgg ccgggtggtc gtcgccaagt tctatcgacc ggggcgctgg
     2701 agcgatgcgc agatcgtcga ggagcacgcg ttcgccgcgg agctcgcggc ggcggaaatt
     2761 ccggtcgccg tgccgctggt cctcggtcgc gacccgcgtt cgatgtacgc ggcgagcgtg
     2821 acgccggtca gcgccacctt ggcgacgacg acgatcggcg gccacgcgta tcgcttcggc
     2881 gtctgcgagc gacttgccgg gcgcgccccc gagctcggcc aacccgacgt gctgcgctgg
     2941 ctcggccgct tcatcggccg catccacgcc gtcggcgcga aggcccgctt cgccgagcga
     3001 ttgacgctcg acgtcgccag ctacggcgag tccagccgcg actggctgct cgagaacgac
     3061 agtgtcacgc ccgacacgtc ggccgtctgg cgcgacctcg tcgacacggc cttgacggcg
     3121 tgtcgcgaag ccttcgagtc ggtgccgggc cggacgcttc gcctgcacgg cgactgccac
     3181 cccggcaacg tgctctggac cgacgcaggc cctcatttcg tcgacctcga cgacgccgtc
     3241 aacggcccgg cgatccagga tctgtggatg ttgttgtcgg gcgatggccc ggccatgtcg
     3301 cagcagcttc tcgacatcct cgagggctac gaatccttca tggaattcga ctggcgcgag
     3361 aggcgactgg tcgagccgct gcgcaccctg cgcatgatcc accacagcgc ctggatcgcg
     3421 aagcgctgga gcgacccggc ctttccgatc gcctttccct ggttcgcgag ccccgcgtac
     3481 tgggctgacc agtgcacgcg actgcgcgaa cagctggcgg caatggaaga cgcgccgccc
     3541 tagtcggtcg gcgaccgcgg agtcgctgcc gacgcgatcg gaccccgacg ccgtcgccgg
     3601 ggcccgaccg aagcgcctca ggcggcaagc agcgaattga cgcggcgcac gtacgacgcc
     3661 ggatcctcga gctggccgcc ttccgcgagc agcgcctggt cgaacaggat gttcgccagg
     3721 tcgtcgaaat gcgtgctcga ctcgaggcgc ctgaccagcg cgtgctcggg gttgatctcg
     3781 aggatcggct cggccttggg cgctccctgt cccgcctgct tcagaagccg tgccaggtgt
     3841 ccgctcatgt cgccttcctc ggcgacgagg caggccggcg agtcgacgag ccgggtggtc
     3901 acgcgaacgt ccttg
//
LOCUS       DASRWS010000428         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1322932, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000428 DASRWS010000000
VERSION     DASRWS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322932"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFF72_08095"
     CDS             <1..332
                     /locus_tag="VFF72_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV93163.1"
                     /translation="PSRGAADPGVVRMLELFRRGNFWVMLSLSEKISKTGRPWDDVVP
                     LAQRLIAAAPDRCVWGSDWPHPVSTKQPPNEGELLELLYRFAPDPATRQKILVDNPAR
                     FFGFEHP"
     gene            329..1204
                     /locus_tag="VFF72_08100"
     CDS             329..1204
                     /locus_tag="VFF72_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZV93164.1"
                     /translation="MKLLSYRHRGTDHWGAASGDGVVDLGRRLPDIASVRALLEGGDA
                     ALQRARDALAGAGPDVALTEIEYRLPVPEPRKIFCIGVNYAHRNAEYKDGSDLPKYPS
                     IFMRVPDSFAGHGGALLQPPESSQLDYEGEIGVVIGTRGRRIARETATRHIGGLTCIN
                     EGTIRDWVHHAKFNVTQGKNFDASGAIGPWIATADEYSNGYADLRVQTRVNGESRQDD
                     TTAHLMFDFAYLISYLSTFTTLEPGDVIATGTPTGAGIRFTPPKFLQPGDVVEVEVSG
                     VGVLRNAVRAEKASS"
     gene            1201..2013
                     /gene="hpaH"
                     /locus_tag="VFF72_08105"
     CDS             1201..2013
                     /gene="hpaH"
                     /locus_tag="VFF72_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HZV93165.1"
                     /translation="MSLTPEQLAQAAQRLDDADRTRTACRQFSLEYPGMTIEDAYAIQ
                     KAWTALKLRQGRRVKGHKIGLTSKAMQRAVGITEPDFGVLLDDMFYRDGAVIPTDRFL
                     GLRVEAELAFVLAKPLAGPDCTLFDVLDATAYVTPALEILDARIQRVDPQTKTTRNVL
                     DTISDNAANAAIVLGGRPFKPATLAADMRRIGAIVSKNGEVEETGLAAGVLNHPGYGI
                     AWLANRLHRHGASLEAGEVVLSGSFIRPLDVVKGDTVVADYGDFGSVSCHFG"
     gene            2021..2806
                     /locus_tag="VFF72_08110"
     CDS             2021..2806
                     /locus_tag="VFF72_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZV93166.1"
                     /translation="MDVLPNAFKRALAAGRPQIGLWSILPDPYVSELVAGAGYDWVLY
                     DTEHTPSDVPHMLRQLQAAAAARPFDGAAPASAVVRPAWNDTVLIKRYLDIGAQSLLL
                     PFVQSAEEAEAAVRAIRYAPAGVRGIGGSTRASAFGRVKDYIARAGDELCLLVQVESG
                     DALERLESIAGVDGIDGVFIGPADLAASLGHPGNPGHPAVRAAIDGAIDRIRACGKAP
                     GILMIDEARAKACLERGALFVAVAIDLFILARGAEAIATRFRA"
     gene            complement(2851..3831)
                     /locus_tag="VFF72_08115"
     CDS             complement(2851..3831)
                     /locus_tag="VFF72_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV93167.1"
                     /translation="MSHAPVRLRLGLAVVLLAIATCLPAVAATTVLPEGTIHIVNTFP
                     AGGPSDIISRLIAQKMQVSLKRTFVVENKPGAGGNVAAEQVAKAPADGSVILTGIDTT
                     FTVNPGIYRSMPFKPGDFKPLMIMASSGLMVATNPSLGVKTLAELVAKGRKQTLTFSS
                     GSNGSPGHLAAAILGEAAGIKVLHVPYKGNSPAVLALLTGEVQAGILATPGLLPHVEA
                     GKLTALAVTSRQRSALAPNVPTVAEAGMKELQVEVLYVAMVPAATPQPVMDVLDKAMA
                     EALAAPDVKAALAKLDLFVEAQTGKAAEERLAAQRVRYERIIKATGMKID"
     gene            3915..4769
                     /locus_tag="VFF72_08120"
     CDS             3915..4769
                     /locus_tag="VFF72_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZV93168.1"
                     /translation="MTAARPSIADKRRAFRSLHESGCFVIPNPWDIGSALYLQGLGFK
                     ALATTSSGFAWSQGRADNAIDRDMALDHLHAMVAATDLPINADFESGFAADAKGVGES
                     VRLAVETGVAGLSIEDSTGDMATPLLAIDEATARVRAARAAIDATGGDTLLVGRAECF
                     LVGRPDLDETIARLKAYAGAGADCLYAPGLRSRDDIRRVVEAVAPRPFNLLIGTASDL
                     TVADVAALGVRRISVGGALARSAWGGFMRAAEALAKEGRFEVFSQAASGKTLNDLFAG
                     RGTAAQAT"
BASE COUNT          725 a         1771 c         1619 g          699 t
ORIGIN      
        1 atccgtcgcg cggcgcggcc gatccgggcg tcgtgcgcat gctcgagctg tttcgccgcg
       61 gcaacttctg ggtgatgctg tcgctttcgg aaaagatctc gaagaccggt cgtccctggg
      121 acgacgtcgt gccgctggcg cagcgcctga tcgccgccgc gcccgatcgc tgcgtctggg
      181 gcagcgactg gccgcatccg gtctcgacca agcagccgcc caacgaaggc gagctgctcg
      241 aactgctcta tcgtttcgca cccgacccgg cgacgcggca aaagatcctc gtcgacaacc
      301 cggcccgctt cttcggcttc gaacatccat gaaacttctc agctaccggc atcgcggcac
      361 ggaccattgg ggcgcggcca gcggcgacgg cgtcgtcgac ctcggtcgcc gcctgcccga
      421 catcgcgtcc gtccgtgcgc tgctcgaagg tggcgacgcc gcgttgcagc gggcacgcga
      481 tgcccttgcc ggcgccgggc ccgacgtggc gctgaccgag atcgaatacc ggttgcccgt
      541 gcccgagccg cgcaagatct tctgcatcgg cgtcaactac gcgcaccgca acgccgagta
      601 caaggacggc agcgacctgc cgaagtaccc gagcatcttc atgcgcgtgc ccgactcctt
      661 cgccggccac ggcggcgcgc tgctgcagcc gccggagtcg agccagctcg actacgaggg
      721 cgagatcggc gtcgtcatcg gcacgcgcgg ccggcgcatc gcccgcgaga cggcgacgcg
      781 ccacatcggc ggcctgacct gcatcaacga aggcacgatc cgcgactggg tgcaccacgc
      841 caagttcaac gtgacgcaag gcaagaactt cgacgcctcg ggcgccatcg ggccatggat
      901 cgcgaccgcc gacgagtact cgaacggcta cgccgacctg cgcgtgcaga cgcgcgtcaa
      961 cggcgagagc cgccaggacg acacgactgc gcatctgatg ttcgacttcg cctacctgat
     1021 ctcgtatctc tcgaccttca cgacgctcga gccgggcgac gtcatcgcca ccggcacgcc
     1081 gaccggcgcc ggcatccgct tcacgccgcc gaagttcctg cagccgggcg acgtcgtcga
     1141 ggtcgaggtc tcgggcgtcg gcgtgctgcg caacgcggtg cgcgcggaaa aggcctcgtc
     1201 atgagcctca cgcccgagca gctcgcgcag gccgcgcaac gcctcgacga cgccgaccgg
     1261 acgcgcaccg cgtgtcgcca gttttcgctc gagtaccccg gcatgaccat cgaggacgcc
     1321 tacgcgatcc agaaggcgtg gacagcgctc aagctgcgcc agggtcgccg cgtcaagggc
     1381 cacaagatcg gcctgacgtc gaaggcgatg cagcgcgccg tcggcatcac cgaacccgac
     1441 ttcggcgtcc tcctcgacga catgttctac cgcgacggcg ccgtcatccc gaccgaccgc
     1501 ttcctcggat tgcgcgtcga ggccgagctc gcgttcgtgc tcgcgaagcc gctcgctggc
     1561 cccgactgca cgctcttcga cgtgctcgac gccaccgcct acgtcacgcc ggcgctcgag
     1621 atcctcgatg cgcgcatcca gcgcgtcgat ccgcagacga agacgacgcg caacgtcctc
     1681 gacaccatct ccgacaacgc cgccaatgcg gcgatcgtgc tcggcggccg gccgttcaag
     1741 ccggcgacgc tagccgccga catgcgccgc atcggcgcca tcgtcagcaa gaacggcgag
     1801 gtcgaggaga cgggcctggc cgcgggcgtg ctcaaccatc ccggctacgg catcgcctgg
     1861 ctcgccaacc ggctgcatcg ccacggcgcc tcgctcgagg ccggcgaagt cgtgctctca
     1921 ggctcgttca tccggcccct cgacgtcgtc aagggcgaca ccgtggtcgc cgactacggc
     1981 gacttcggca gcgtctcctg ccacttcgga taagccggcc atggacgtcc ttcccaacgc
     2041 cttcaagcgc gcgctcgcgg ccggccggcc gcagatcggc ctctggtcga tcctgcccga
     2101 cccgtacgtg tcggagctcg tcgccggtgc cggctacgac tgggtgctct acgacaccga
     2161 gcacacgccg agcgacgtgc cgcacatgtt gcgccagctg caagccgcgg ccgcggcgcg
     2221 gcccttcgac ggcgccgcac cggcctcggc ggtggtgcgc ccggcgtgga acgacaccgt
     2281 gctcatcaag cgctacctcg acatcggcgc gcagagcctg ctgctgccct tcgtgcagag
     2341 cgccgaggaa gcggaagcgg cggtgcgcgc gatccgctat gcaccggccg gcgtgcgcgg
     2401 catcggcggc tcgacccggg cgagcgcctt cggccgcgtc aaggactaca tcgcccgcgc
     2461 cggcgacgag ctctgcctac tcgtccaggt ggagtcgggc gacgcgctcg agcggctcga
     2521 atcgatcgcc ggggtcgacg gcatcgacgg cgtcttcatc ggcccggccg atctcgcggc
     2581 aagcctcggc catccgggca acccggggca tccggcggtg cgcgccgcga tcgacggcgc
     2641 catcgaccgc atccgcgcct gcggcaaggc gccgggcatc ctgatgatcg acgaggcccg
     2701 ggcgaaggcc tgcctcgaac gcggcgcctt gttcgtggcc gtggccatcg acctgttcat
     2761 cctcgcgcgc ggtgccgagg cgattgcgac gcgcttcagg gcctgaaccg aaagctgcgt
     2821 cgctctgcgg cgcgacgacc gagacgcagc tcagtcgatc ttcatgccgg tcgccttgat
     2881 gatccgctcg tagcggacgc gctgggcggc gaggcgctct tcggccgcct tgccggtctg
     2941 cgcctcgacg aagaggtcga gcttggccag cgccgccttc acgtcgggcg cggccagcgc
     3001 ctcggccatc gccttgtcga gcacgtccat caccggctgg ggtgtcgccg ccggcaccat
     3061 cgcgacgtag agcacctcga cctgcagctc cttcatgccc gcctcggcga cggtgggcac
     3121 attcggcgcg agcgccgatc gctgccggct cgtcaccgcc agcgccgtga gcttgccggc
     3181 ctcgacgtgc ggcagcagcc ccggcgtcgc caggatgccc gcctgcacct cgccggtcag
     3241 cagcgccagc accgccggcg agttgccctt gtacggcacg tgcagcacct tgatgcccgc
     3301 cgcctcgccg aggatcgcgg cggcgaggtg accggggctg ccgttgctgc ccgagctgaa
     3361 ggtgagggtc tgcttccggc ccttggcgac gagctcggcc agcgtcttga cgccgagcga
     3421 cggattcgtg gcgaccatga ggcccgacga cgccatgatc atcagcggct tgaagtcgcc
     3481 gggcttgaac ggcatcgagc gatagatgcc cggattgacg gtgaacgtcg tgtcgatgcc
     3541 ggtgaggatg acgctgccgt cggccggcgc cttggcgacc tgctcggccg cgacgttgcc
     3601 accggcgccc ggcttgttct cgacgacgaa ggtccgcttc agcgagacct gcatcttctg
     3661 cgcgatgagg cgcgagatga tgtccgacgg gccgcccgcc gggaaggtgt tgacgatgtg
     3721 gatcgtcccc tcgggcagca cggtcgtggc cgcgacggcg ggcaggcacg tcgcgatcgc
     3781 gagcagcacg acggcaagac cgaggcgaag gcggacaggc gcgtggctca tggcgcagga
     3841 tgcagtgcgg aggtcgatcc atcgtacgcg ctcgcgcgca aaatcgcccc atgcaaacca
     3901 cgaggaccga tccgatgacc gcagcccgcc cgagcatcgc cgacaagcgc cgcgcctttc
     3961 gctcgctgca tgaaagcggc tgcttcgtca tcccgaaccc gtgggacatc ggcagcgccc
     4021 tctacctgca aggcctcggc ttcaaggccc tggcgacgac gagctcgggc ttcgcgtggt
     4081 cgcaaggccg tgccgacaat gcgatcgatc gagacatggc gctcgaccat ctccacgcga
     4141 tggtcgcggc caccgacctg ccgatcaacg ccgacttcga gagcggcttc gccgccgatg
     4201 cgaaaggcgt cggcgagagc gtgcggctgg ccgtcgagac gggtgtcgcc ggcctgtcga
     4261 tcgaggactc gaccggcgac atggcgacgc cgctccttgc catcgacgaa gcgacggccc
     4321 gcgttcgcgc cgcgcgtgcc gccatcgacg cgaccggcgg cgacacgctg ctggtcggcc
     4381 gcgccgagtg tttcctcgtc ggtcgccccg acctcgacga gacgatcgcc cgcctgaagg
     4441 cctacgccgg cgccggcgcc gactgcctct acgcgcccgg ccttcgctcg cgcgacgaca
     4501 tccgtcgcgt cgtcgaggcg gtcgcgccac ggcccttcaa cttgctgatc ggcacggcca
     4561 gcgatctgac cgtggccgac gtcgcggcgc tcggcgtgcg ccgcatcagc gtcggcggcg
     4621 cgctggcgcg aagcgcctgg ggcggcttca tgcgcgcggc cgaagcgctg gcgaaggaag
     4681 gccgcttcga ggtcttctcg caggcagctt ccggaaagac gctgaacgac ctgttcgccg
     4741 ggcgcggcac cgcggcgcag gcgacgtagc ggcgggatag caccgcttcg ccgcgcacac
     4801 gcccgtcacc ggca
//
LOCUS       DASRWS010000429         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1323323, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000429 DASRWS010000000
VERSION     DASRWS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323323"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2362
                     /locus_tag="VFF72_08125"
     CDS             <1..>2362
                     /locus_tag="VFF72_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93169.1"
                     /translation="VPAQLGWPALEAQATRGTTRGWMGQAVLTGFHVVTGLVKDPAAD
                     LLAAAVTRKVDGQVDAGVYALGAQALAPLKGNGRKLDAVPAANDGAPLLVLVHGTFSD
                     TAGTFGKLWSQHAPVVRQLFSRYADRVYGFDHPTLGSSPIGNALMLVRALPAGARLHL
                     LTHSRGGLVAEVLARACAPGADDAPSAAFAGTGYATHRAELRALLDEARAKKLRVERV
                     VRVACPSRGTLLASKRLDAYLSILAWCLELAAIPVAPQLVDFLHEVARRRADPTELPG
                     LEAMLPTSPVIAWLNSASDAIPGELRVVAGDMAGDSIGSWVKTLLADAFYWTDNDLVV
                     QTRSMYGGAPRAASTGGAASFLLDRGGKVSHFAYFANDRTVQAIAAALCDDAPDVFAA
                     IGPLSWAGEDASGSRGALLAARPRGAAAADAATRPAVFVIPGILGSNLAIDGKRIWLG
                     FRFLNGMARLAWDPKTASHVSADGPIDHVYGDLIERLADTHEVIAFAFDWRRPIEDEA
                     HRLADALDAALAARQASGQPVRIVAHSMGGLVARTMALERPETWRNLLARSGGRLLML
                     GTPNGGSWSPMQTLSGDDTFGNALAAFGSLLDNAGARRTMAGMPGFLQLQAALLDPAL
                     RLDRAESWQKLADDDMARLAGRSVWHLEDVQRTIYQWGAPPQAVLDAAVALRKRLDAQ
                     AAALGGDAKSMLLVVGHASFTPGGYIFGDEGLEYVDAPGGGDGRVPIGCAVLPGVRTW
                     KIDAAHGDLPGTTSAFAGYVELLTQGDTRLLETLDPAGLVAANRSAV"
BASE COUNT          299 a          855 c          851 g          357 t
ORIGIN      
        1 gtccccgcac agctcggctg gccggcgctc gaagcgcagg caacgcgggg cacgacccgc
       61 ggctggatgg ggcaggccgt gctgaccggc tttcacgtcg tcaccggcct cgtcaaggac
      121 ccggcggccg atctgctcgc cgctgccgtc actcgcaagg tcgacggaca ggtcgacgcc
      181 ggcgtctacg cgttgggcgc gcaggcgctg gcgccgctca agggcaacgg ccgcaagctc
      241 gatgccgtgc cggccgcgaa cgatggcgcg ccgctgctgg tgctcgtcca cggcacgttc
      301 agcgacacgg ccggcacctt cggcaagctc tggtcgcagc atgcgccggt cgtgcgccag
      361 ctcttctcgc gctacgccga tcgggtctac ggcttcgatc atccgaccct cggcagcagt
      421 ccgatcggca acgccttgat gctggtgcgg gcgctgccgg ccggcgcgcg tctgcatctg
      481 ctcacccatt cgcgtggtgg cctggtggcc gaagtgctgg cgcgcgcctg cgcccccggc
      541 gcagacgatg caccgtcggc ggcgttcgcc ggcaccggct acgcaacgca tcgggccgag
      601 cttcgcgccc tgctcgacga agcccgcgcg aagaagctgc gcgtcgaacg ggtcgtgcgc
      661 gtcgcctgcc cgtcacgcgg cacgctcctc gcctcgaagc ggctcgatgc ctatctgtcg
      721 atcctcgcct ggtgcctcga gctcgccgcg attccggtcg cgccgcagct tgtcgacttc
      781 ctgcacgagg tggcgcgccg gcgcgccgat ccgaccgagc tgccgggcct cgaggcgatg
      841 ctgccgacga gcccggtgat cgcctggctg aacagcgcgt ccgatgccat cccgggcgag
      901 ctgcgtgtcg tcgccggcga catggcgggc gattcgatcg gctcgtgggt gaagacgctg
      961 ctggccgatg cgttctactg gaccgacaac gatctggtcg tgcagacgcg gtcgatgtac
     1021 ggaggcgctc cgcgcgccgc ctcgaccggc ggcgcggcga gcttcctgct cgatcgtggc
     1081 ggcaaggtct cgcacttcgc ctatttcgcc aacgaccgca ccgtgcaggc gatcgccgcg
     1141 gcgctgtgcg acgacgcgcc ggacgtgttc gccgcgatcg gtccgctctc gtgggccggc
     1201 gaggacgcca gcggcagccg cggcgcgctt ctggcggcgc gaccccgcgg cgcggccgcg
     1261 gccgacgcgg cgacacggcc ggcggtgttc gtgatcccgg gcatcctcgg ctccaacctg
     1321 gcgatcgacg gcaagcgcat ctggctcggc tttcgctttc tcaacgggat ggcccggctc
     1381 gcgtgggatc cgaagaccgc gtcgcacgtc tcggccgacg ggccgatcga tcacgtctac
     1441 ggcgatctga tcgagcgcct cgccgacacg cacgaggtga tcgccttcgc gttcgactgg
     1501 cgccggccga tcgaggacga agcgcatcgc ctcgccgacg cgctcgacgc ggcgctcgcg
     1561 gcacggcagg cgagcggcca gccggtccgc atcgtcgccc actcgatggg cggcctggtg
     1621 gcgcggacga tggcgctcga gcggcccgag acctggcgca atctgctggc gcgctccggc
     1681 ggccggctgt tgatgctcgg cacgccgaac ggcggctcgt ggtcgccgat gcagaccctc
     1741 tcgggcgacg ataccttcgg caacgcgctg gccgcgttcg gctcgctgct cgacaacgcc
     1801 ggcgcccgcc ggacgatggc cggcatgccg ggcttcctgc agctgcaggc ggcgctgctc
     1861 gatccggcgt tgcgcctgga tcgtgcggag agctggcaaa agctcgccga cgacgacatg
     1921 gcgcgactcg ccgggcgcag cgtctggcat ctcgaggacg tgcagcgcac gatctaccaa
     1981 tggggcgcgc cgccgcaagc ggtcctcgat gcggcggtag cgctgcgcaa gcgcctcgac
     2041 gcccaggcgg ccgcgctcgg tggcgacgcg aagtcgatgc tgctggtggt cggccacgca
     2101 tccttcacgc cgggcggcta catcttcggc gatgagggac tcgaatacgt cgacgccccg
     2161 ggcggcggcg acggccgggt gccgatcggc tgcgccgtgc tgccgggtgt tcgcacctgg
     2221 aagatcgatg ccgcgcacgg cgacctgccc ggcacgacga gcgccttcgc cggctatgtc
     2281 gaactgctga cgcagggcga tacgcgcctg ctggagactc tcgatccggc cggtctcgtg
     2341 gcggcgaatc gctcggcggt ct
//
LOCUS       DASRWS010000430         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2748039, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000430 DASRWS010000000
VERSION     DASRWS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748039"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFF72_08130"
     CDS             complement(<1..223)
                     /locus_tag="VFF72_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HZV93170.1"
                     /translation="MPQLAANLSWIYQEVPFLQRFGAAAAHGFKAVEILFPYEAPAKD
                     IAVELKKHKLTQALFNLPAGDPSKGERGFA"
     gene            complement(232..972)
                     /gene="otnC"
                     /locus_tag="VFF72_08135"
     CDS             complement(232..972)
                     /gene="otnC"
                     /locus_tag="VFF72_08135"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HZV93171.1"
                     /translation="MDGVGRQQAAAAGAEIRQFRCTRFLHQGVCKAMSSEAKSREDVC
                     ALGRKLAARGLCPGTSGNISVRIADGWLMTPTNSSLGELDPAQLSKLDTNGKHVGGDP
                     PTKEALLHRSVYDVRPKDGAIVHLHCTHAVAISCLDPSCHHNTETTLPPITPYFVMRV
                     GKLPLVPYFKPGDPKLADAIRDYAKGHRAVLLANHGPVVAGTSLSAASASIEELEETA
                     KLHLLLQGFKVRLLSNAQVREVEEAFPS"
     gene            complement(873..2129)
                     /gene="otnK"
                     /locus_tag="VFF72_08140"
     CDS             complement(873..2129)
                     /gene="otnK"
                     /locus_tag="VFF72_08140"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HZV93172.1"
                     /translation="MAILGIIADDFTGATDIAGMLVKNGMRTIQTIGVPAKGTIPDDV
                     DAVVVALKTRSIAAKDATAQSLDALKTLQAAGCVRFFFKYCSTFDSTDRGNIGPVGDA
                     LLDALGGKQAIYCPAFPENARTIFFGHLFVGDLLLSDTHMRHHPLNPMTDSSLVRVLA
                     RQTKHKVGLVPLKQVQAGSATLRAALDKLASDGVRHVIVDAVADTDLGIIGEAVGDEV
                     LVTGGSGVALGLPAAYRRRGLLAHKSGADALPTIGGSAAVLAGSCSAATLGQIANFKG
                     AHLALDTDAICRGEPVGDKALAWARGKLGNGPILLSASDKPEAVKALQQKYGVEKSSH
                     AIEGTMAQLAKGLVAAGVGRLVVAGGETSGAVVGALDVTALRIGPEIDPGVPWTASVG
                     SKPLLLALKSGNFGAPDFFTKAFAKL"
BASE COUNT          357 a          739 c          796 g          385 t
ORIGIN      
        1 cggcgaaccc gcgctctcct ttgctcgggt cgccggccgg caggttgaac agcgcctgtg
       61 tgagcttgtg cttcttgagc tcgacggcga tgtccttcgc cggcgcctcg taggggaaca
      121 ggatctcgac ggccttgaag ccgtgcgcgg cggcagcgcc gaagcgctgc aggaagggca
      181 cctcctggta gatccaggag agattggcgg cgagttgggg catgggtcgt cctacgaagg
      241 gaatgcttct tcgacttcgc gcacctgcgc gttgctcaac aggcgcacct tgaagccctg
      301 cagcagcaga tggagcttgg cggtctcctc gagctcctcg atggaggcgg aggcggcgga
      361 aagcgaggtg ccggcgacga ccgggccgtg attcgcgagc agcacggcgc gatggccctt
      421 ggcgtagtcg cggatcgcgt cggccaattt ggggtcgccc ggcttgaagt agggcaccag
      481 cggcagcttg ccgacgcgca ttacgaagta gggcgtgatc ggcggcagtg tggtttccgt
      541 gttgtgatgg cacgacggat cgaggcagga gatcgccacc gcgtgcgtgc aatgcagatg
      601 cacgatcgcg ccgtccttgg gccgcacgtc gtagaccgag cgatgcagca gcgcctcctt
      661 ggtcggcgga tcgccgccga catgcttgcc gttggtgtcg agcttggaga gctgcgccgg
      721 atcgagctcg cccaaggagg aattggtcgg cgtcatcagc cagccgtcgg cgatccgcac
      781 gctgatgttg cccgaggtgc cggggcaaag tccacgcgcg gcgagcttgc ggccgagcgc
      841 gcagacgtct tcacgggatt tggcttcgct gctcatagct ttgcaaacgc cttggtgaag
      901 aaatcgggtg caccgaaatt gccggatttc agcgccagca gcagcggctt gctgccgacc
      961 gacgccgtcc acggcacgcc gggatcgatc tccggcccga tgcgcagcgc cgtcacgtcg
     1021 agcgcgccga ccaccgcgcc cgaggtctcg ccgcccgcaa caacgagccg gccgacgccc
     1081 gccgcgacca ggcccttcgc aagttgcgcc atggtgccct cgatcgcatg gctcgatttc
     1141 tcgacgccgt atttctgctg cagcgccttc accgcctcgg gcttgtcgct ggccgacagc
     1201 aggatcgggc cgttgccgag cttgcccctg gcccaggcga gcgccttgtc gccgaccggc
     1261 tcgccgcggc agatcgcatc ggtgtcgagc gccaggtgcg cgcccttgaa gttggcgatc
     1321 tggccgagcg tcgcggcgga gcacgagccc gccagtaccg ctgccgagcc gccgattgtc
     1381 ggcagcgcgt cggcgcctga tttgtgggcg agcagaccgc ggcggcgata ggcggcgggc
     1441 aggccgagcg caacgcccga gccgccggtc accagtactt cgtcgccgac cgcctcgccg
     1501 atgatgccga gatcggtgtc ggcgacggca tcgacgatca catgccgcac gccatcgctc
     1561 gcgagcttgt cgagcgccgc gcgcaaagtc gcggagcccg cctgcacctg cttcagcggg
     1621 accagcccga ccttgtgctt ggtctgccgc gccagcacgc gcaccaggct cgaatccgtc
     1681 atcggattga gcgggtggtg ccgcatgtgc gtgtcggaga gcagcaggtc gccgacgaac
     1741 agatggccga agaagatggt gcgggcgttc tcggggaaag cggggcagta gatcgcctgc
     1801 ttcccgccca gcgcatcgag cagcgcatcg ccgaccgggc cgatattgcc ccgatccgtc
     1861 gaatcgaagg tcgagcaata cttgaagaag aagcgcacgc agccggccgc ttgcagcgtc
     1921 ttcagtgcgt cgagcgactg agcggtcgcg tccttggcgg cgatcgagcg cgtcttcagc
     1981 gcgaccacga cggcatcgac gtcgtcgggg atcgtgccct tcgctggcac accgatggtc
     2041 tggatcgtcc gcatgccgtt cttgaccagc atgcccgcga tgtcggtcgc gccggtgaag
     2101 tcgtcggcga tgattcccag gatggccatg gttgacccta ggcgatcacg gtcgcgggca
     2161 tccagaactt tcgccgccgg ctgcggcgac gttgctcgtt ctcgccgtca tagacgtgca
     2221 gcggcggcgc cggatcgaac gaatcgcgga tctccagcgc attgacgttg agggtgg
//
LOCUS       DASRWS010000431         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1881546, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000431 DASRWS010000000
VERSION     DASRWS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881546"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFF72_08145"
     CDS             complement(<1..899)
                     /locus_tag="VFF72_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV93173.1"
                     /translation="MTTSHATAHVDTFARDHLPSPASQPEFLFELPELKFPARLNCAT
                     ELLDRHVAEGRGGRMCVRSPGGPAWTYADLQRQADRIAQVLVSEMGLVPGNRVLLRAA
                     NKPMLVACWFAVMKAGGIAVGTMPMLRAKELGQIVEKAQVTIALCDTALRGELAAAAA
                     AAPMLSRIAYFGDPAAADGLEARMARHDAPFTNVDTAADDTCLLAFTSGTTGQPKATM
                     HFHRDVMASCVCFPTHVLRAVPDDVFIGSPPLAFTFGLGGLTVFPMYIGASTVLLEKA
                     GPPDLLVAIVAERATVCFTAPTS"
     gene            complement(959..2140)
                     /locus_tag="VFF72_08150"
     CDS             complement(959..2140)
                     /locus_tag="VFF72_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZV93174.1"
                     /translation="MASSKASFHWDDPLLLEQQLTGEERAVREAARSYCEARLLPRVL
                     DAFRNGTTDPAIFREMGELGLLGSTIPEAYGGGGMNYVCYGLVAREVERIDSGYRSMM
                     SVQSSLVMVPINEFGTEAQKQKYLPKLASGEWIGCFGLTEPNHGSDPGSMVTRAKKVA
                     GGYSLSGSKMWITNSPIADVFVVWAKDDEGAIRGFILDKGAKGLSTPPIHGKVGLRAS
                     ITGEIVMDEVFCAEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCFHRARQYVL
                     DRKQFGKPLAANQLVQKKLADMETEITLGLQGCLRLGRMKDDGTASVEVTSILKRNSC
                     GKALDIARTARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAITGISA
                     F"
     gene            complement(2159..2554)
                     /locus_tag="VFF72_08155"
     CDS             complement(2159..2554)
                     /locus_tag="VFF72_08155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV93175.1"
                     /translation="MNEALLPEGWPRPKGYANGVAARGRQVHVAGQVGWNPAGVFETD
                     DFAAQARQAFANVVAVLHTAGAKPEHIVRMTWYVTSKREYLAAGREIGKAYREIIGFY
                     DIAMTAVEVSALIEDRAKVEIEVTAVIPD"
     gene            complement(2551..3762)
                     /locus_tag="VFF72_08160"
     CDS             complement(2551..3762)
                     /locus_tag="VFF72_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV93176.1"
                     /translation="MSDGSYLDWPFFEPRHAALARELDAWAEANVRDAHGQDVDAVCR
                     SLVRSLGDAGWLGHAVGGRAHGGREETIDTRAICLVRETLARHSGLADFAFAMQGLGS
                     GAISLAGTEEQKAAYLPRVARGEKIAAFALSEPDAGSDVAAMQCAARADGTDYVLDGE
                     KTWISNGGIADFYVVFARTGEPGAAPARRGAKGISAFIVDAGLPGFSIAGRIDVIAPH
                     PLARLKFTGCRIPASRRIGAEGEGFKIAMRTLDVFRTSVAAAALGFARRALDEAMARA
                     TTRRMQDGVLADFQLTQASLAQMAMTIDSAALLTYRAAWQRDQGRGVTREAAMAKYAA
                     TEGAQRVIDAAVQIWGGLGVVSEEPVERLYREIRALRIYEGASEVQLLIIARELLKEH
                     TQGVATKEKTQ"
     gene            complement(3773..4636)
                     /locus_tag="VFF72_08165"
     CDS             complement(3773..4636)
                     /locus_tag="VFF72_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZV93177.1"
                     /translation="MSHESTPRVDPALVAGNHRPAADYEAKHFLWNVDAGVARIVLDR
                     PERKNPLTFDSYAELRDLFGNLKYANDVKAVVVSGAGGNFCSGGDVHEIIGPLVRLKA
                     PELLMFTRMTGDLVKAMRACPQPIVAAIDGVCAGAGAIMAMASDLRVGTARSKTAFLF
                     NRVGLAGCDMGACAMLPRIVGQGRASELLFTGRALGGEEAERWGFFNRLVDPARLEED
                     AVALARDLVAGPTFANGITKTMLHQEWAMTIEQAIEAEAQAQALCMLTQDFTRAYEAF
                     AAKQKPVFQGN"
BASE COUNT          676 a         1593 c         1769 g          742 t
ORIGIN      
        1 tacgaggtgg gcgcggtgaa gcagacggtg gcgcgctcgg cgacgatggc cacgagcagg
       61 tcgggcgggc cggccttctc gagcagcacg gtcgacgcgc cgatgtacat cggaaagacc
      121 gtcaggccgc cgaggccgaa agtgaaggcc agcggcgggc tgccgatgaa gacgtcgtcg
      181 ggcacggcgc gcagcacgtg cgtcgggaag cacacgcagc tcgccatcac gtcgcggtgg
      241 aagtgcatgg tggccttcgg ctggccggtc gtgcccgagg tgaaggcgag caggcacgtg
      301 tcgtcggcgg cggtgtcgac gttggtgaac ggcgcgtcgt ggcgggccat gcgcgcctcg
      361 aggccatcgg ccgcggccgg gtcgccgaaa tacgcgatgc gcgagagcat cggcgccgcg
      421 gcggcggcgg cggcgagctc gccgcgcagc gccgtgtcgc agagcgcgat cgtcacctgt
      481 gccttctcga cgatctggcc gagctccttg gcgcgcagca tcggcatcgt gccgacggcg
      541 atgccgcccg ccttcatcac cgcgaaccag caggcgacga gcatcggctt gttggcggcg
      601 cgcagcagca cgcggttgcc gggcacgagg cccatctcgc tcaccagcac ctgggcgata
      661 cggtcggcct ggcgctgcag gtcggcatac gtccaggccg ggccgccggg cgagcgcacg
      721 cacatgcggc cgccgcggcc ttcggccacg tgccggtcga gcagctcggt ggcgcagttg
      781 agccgggccg ggaatttcag ctcgggcagc tcgaacagga actcgggctg gctcgcgggc
      841 gacggcaggt ggtcgcgcgc gaaggtgtcg acgtgggcgg tggcgtggga ggtggtcatg
      901 gcagtgaggg cgaggcggga accatgaatg cggacgaacg gagaagatgg cgtgccgatc
      961 agaacgccga gatcccggtg atggcgcggc cgaggatcag cgcgtgcacg tcgtgcgtgc
     1021 cctcgtaggt gttgacgacc tcgaggttga cgaggtggcg cgccacgccg aattcgtcgc
     1081 tgatgccgtt gccgcccatc atgtcgcgcg cggtgcgggc gatgtcgagc gccttgccgc
     1141 aggagttgcg cttgaggatc gaggtgactt ccaccgaggc ggtgccgtcg tccttcatgc
     1201 ggcccaggcg gaggcaaccc tgcaggccga gcgtgatctc ggtttccatg tcggcgagct
     1261 ttttctgcac cagctggttg gcggcgagcg gcttgccgaa ctgcttgcgg tcgagcacgt
     1321 actggcgggc acgatggaag cagtcttcgg cggcgccgag cgcgccccag gcgatgccgt
     1381 agcgggccga gttgaggcag gtgaacgggc ccttcaggcc gcgcacctcg ggaaaggcgt
     1441 tctcctcggc gcagaacacc tcgtccatga cgatctcgcc ggtgatcgag gcgcgcaggc
     1501 cgaccttgcc gtggatcggc ggcgtgctca ggcccttggc gcccttgtcg aggataaagc
     1561 cgcggatcgc gccctcgtcg tccttggccc agaccacgaa cacgtcggcg atcggcgagt
     1621 tggtgatcca catcttgctg cccgagagcg aatagccgcc ggccaccttc ttcgcccgcg
     1681 tgaccatgct gccggggtcg gagccgtggt tgggctcggt gaggccgaag cagccgatcc
     1741 attcgcccga ggcgagcttg ggcaggtact tctgcttctg cgcctcggtg ccgaactcgt
     1801 tgatcggcac catcaccagc gagctctgta cgctcatcat cgagcgatag cccgagtcga
     1861 tgcgctcgac ctcgcgcgcg acgaggccgt agcagacgta gttcatgccg ccgccgccgt
     1921 aggcctcggg gatcgtcgag ccgagcaggc cgagctcgcc catctcgcga aagatcgccg
     1981 ggtcggtcgt gccgttgcga aaggcgtcga gcacgcgcgg cagcaggcgc gcctcgcagt
     2041 agctgcgcgc ggcctcgcgc acggcgcgct cctcgcccgt gagctgctgc tcgagaagaa
     2101 gcggatcgtc ccagtggaag gaggctttgg atgaggccat ggcgtgcttt cgaaggaatc
     2161 agtcggggat gacggcggtg acttcgatct cgaccttggc ccggtcctcg atcagtgccg
     2221 agacctcgac ggccgtcatc gcgatgtcgt agaagccgat gatctcgcga taggccttgc
     2281 cgatctcgcg gccggccgcc aggtattcgc gcttgctggt gacgtaccag gtcatgcgca
     2341 cgatgtgctc gggcttggcg cccgcggtat gcagcacggc gacgacgttg gcgaacgcct
     2401 ggcgcgcctg cgccgcgaag tcgtcggtct cgaagacgcc ggccggattc cagccgacct
     2461 ggccggcgac gtggacctgg cggccgcgcg cggccacgcc gttggcgtag cccttcgggc
     2521 gcggccagcc ctcgggcagc agggcttcgt tcattgcgtc ttctccttgg tggccacgcc
     2581 ttgcgtgtgt tccttcagca gctcgcgggc gatgatgagc agctgcacct cgctcgcgcc
     2641 ttcgtagatg cgcagcgccc ggatctcgcg gtacagccgc tcgacgggct cttcgctgac
     2701 gacgccgagg ccgccccaga tctgcaccgc cgcgtcgatg acgcgctgcg cgccttcggt
     2761 ggcggcgtac ttggccatcg ccgcctcccg ggtgacgcca cggccctggt cgcgctgcca
     2821 ggcggcgcga taggtgagga gcgcggcgct gtcgatcgtc atcgccatct gcgcgaggct
     2881 ggcctgcgtg agctggaaat cggcgagcac gccgtcctgc atgcgccgcg tcgtggcgcg
     2941 cgccatcgct tcgtcgaggg cgcggcgggc gaagccgagc gccgccgcag cgaccgaggt
     3001 gcgaaagaca tcgagcgtgc gcatcgcgat cttgaagccc tcgccctcgg cgccgatgcg
     3061 gcgcgaggcg gggatgcggc agcccgtgaa cttcaggcgc gcgagcggat gcggcgcgat
     3121 cacgtcgatg cgcccggcga tcgagaagcc cggcagcccg gcgtcgacga tgaaggccga
     3181 gatgcccttg gcgccgcgcc gggccggagc cgcgccgggc tcgccggtgc gtgcgaacac
     3241 gacgtaaaaa tcggcgatgc cgccgttcga gatccaggtc ttctcgccgt cgagcacgta
     3301 gtcggtgccg tcggcgcggg cggcgcattg catggccgcc acgtcggagc cggcatcggg
     3361 ctccgacagc gcgaacgcgg cgatcttctc gccacgcgcc acgcgcggca ggtaggcggc
     3421 cttctgttcc tcggtgccgg ccaggctgat cgcgcccgag ccgaggccct gcatcgcgaa
     3481 tgcgaagtcg gccaggcccg aatggcgcgc cagcgtctcg cgcacgaggc agatggcgcg
     3541 cgtgtcgatc gtctcctccc tgccgccgtg cgcgcgtccg cccacggcat ggccgagcca
     3601 gccggcatcg ccgagcgaac gcacgagcga gcggcagacc gcgtcgacgt cctggccgtg
     3661 cgcgtcgcgc acgttcgcct cggcccaggc gtcgagctca cgcgcgagcg cggcgtggcg
     3721 cggctcgaag aagggccagt cgagatagct gccgtcgctc atgacacgcg cctcagttgc
     3781 cctggaacac gggcttttgc ttcgccgcga aggcttcgta ggcgcgcgtg aagtcctgcg
     3841 tcagcatgca gagggcctgc gcctgcgcct cggcctcgat cgcctgctcg atcgtcatcg
     3901 cccactcctg gtgcagcatg gtcttggtga tgccgttggc gaaggtgggc ccggcgacga
     3961 gatcgcgcgc cagcgccacc gcgtcttcct cgagccgcgc cgggtcgacg aggcggttga
     4021 agaagcccca gcgctcggcc tcttcgccgc cgagggcgcg gccggtgaag agcagctcgc
     4081 tggcgcggcc ctggccgacg atgcgcggca gcatcgcgca ggcgcccatg tcgcagccgg
     4141 ccaggccgac gcggttgaag agaaacgccg tcttcgatcg cgccgtgccg acgcgcaggt
     4201 cggaggccat cgccatgatc gcgccggcgc cggcgcagac gccgtcgatc gccgcgacga
     4261 tgggctgcgg gcaggcgcgc atcgccttga cgagatcgcc cgtcatgcgc gtgaacatca
     4321 agagctcggg cgccttcagg cgcaccagcg ggccgatgat ctcgtgcacg tcgccgcccg
     4381 agcagaagtt gccgccggcg ccgctcacga ccaccgcctt cacgtcgttg gcgtacttca
     4441 ggttgccgaa gagatcgcgc agctcggcgt acgagtcgaa ggtgagcggg ttcttgcgct
     4501 cggggcgatc gaggacgatg cgggcgactc cggcatcgac gttccagagg aagtgcttcg
     4561 cctcgtagtc ggcggcggga cggtggttgc cggcgacgag ggccggatcg acgcgtgggg
     4621 ttgattcgtg gctcattttt gagaagtctc gttcgcctct ggggttcgtc aagcgtggca
     4681 tcgagcggcg cggcgggcgg tgcccggcgc gggctcatgc cgtggcggtc ggcgctgcgc
     4741 gccgacttca ctgcgctgct cgcttcgggg tcgcgccgcg
//
LOCUS       DASRWS010000432         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3060809, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000432 DASRWS010000000
VERSION     DASRWS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060809"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFF72_08170"
     CDS             complement(<1..828)
                     /locus_tag="VFF72_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZV93178.1"
                     /translation="MIGSSLTLNVLKKRADKLADRRRFFHKTGGFAVGMAGGAILAAC
                     GGNSNDATAQSAVSDVDVLNFALNLEYLEAQFYSYAVNGTGLAANMTTGTGTQGAVIA
                     GHAVPFTDPIVQQYAKEIAADELAHVNFLRSALGASAVAQPQIDVGFSDPNGAFSTAA
                     RAAGLVGAGVAFDPYANDETFLLGAFIFEDVGVTAYKGASPLISNKTYLEAAAGILAA
                     EAYHAGLVRSTLYSKGLATPSLRTSADAISDARDSLDGPTDDDQGISETAGGASNIAP
                     "
     gene            1147..2775
                     /locus_tag="VFF72_08175"
     CDS             1147..2775
                     /locus_tag="VFF72_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZV93179.1"
                     /translation="MSAESWKFAPAYELPCWPFVAPPDLGSGARIRYPVVIAGGGLAG
                     LTLACDLALRGVRAVLLDEDDTVGVRGASSRGICYAQKSLEIFDRLGLYERIRDKGIT
                     WSVGRTFSGADEIYAFNLDDESQSEQPPFINLQQFYLEWFLVERILEIGGTDVRWKSR
                     VATVENGADGVVVEVQTPAGAYSLECDWFIDATGANSRIRDAMGLQTHASRGTDRWCI
                     SDVRFKVPFAVERWTWIDAPFNEGRAVWQHLMADDVWRLDYQMGGDADPEAISKPEVA
                     GARLRQQLGPGVEFEFVWIGPYGYRDHLLETFRVGRTMFIGDAAHVVSPFGARGGNNG
                     IQDAANLGWKLALVLEEQAGDALLDSYHVERYEAAAENLKVTSRTSRFLAPRSAAEHR
                     IRRAVTALARRHEFARRLSNTGRMAQANDYSPSPWSGAGARSVQNIAFDDTTLMRCLG
                     GGTRFLGFVVDAGPALEGLAQLGARWPVAFHAIDTASPLAAHLGVSAGDVVLVRPDAY
                     VAARLRADDPVSVEAALRAALSRDEPTGAPGAAA"
     gene            2819..3040
                     /locus_tag="VFF72_08180"
     CDS             2819..3040
                     /locus_tag="VFF72_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HZV93180.1"
                     /translation="MKTHANIPDADGFYEAWLSAHEGLSDEQSTDLDARLVLLLANQV
                     GDQAVLLDCIAEARRTAVPPAALSVAKPT"
     gene            complement(3018..4205)
                     /locus_tag="VFF72_08185"
     CDS             complement(3018..4205)
                     /locus_tag="VFF72_08185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZV93181.1"
                     /translation="MTSEAGSASDKEIAESAGPTAEACLAASPVPRLRRRTLLQAAWP
                     AFGLGPLLARGATPPVPIADMHSHYGILRRPTLPSATFAAELRAARIALIAWAMPADL
                     GWTRTEPSGIVQKSVPPRGELATAFRRRLDRMRAYVARSGLRAVLTRADVDACMAGDS
                     GVILASEGADFLEGRVDALAGYVDMGLRHLQLVHYIRTPIGDFQTVQPTHGGLSDAGH
                     QLVEACNEQGVLVDLAHCSGPAVEQALSVATKPLVWSHGWVDRTEGSWQDRSGFLRRR
                     LSLDLARKIADRGGVVGLWGLGLRRPGPSRTPGEGNWTVGRDDASGYARELAHLVDWL
                     GADHVGIGTDLEGVGDSESVSDYAQVRAVIDALQAMKLPAATVEKVAALNYVRVLKSA
                     LRR"
     gene            4261..>4519
                     /locus_tag="VFF72_08190"
     CDS             4261..>4519
                     /locus_tag="VFF72_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93182.1"
                     /translation="MLQLHYFPGNASLIAHVVLEELGVPFELKYVDRAVSAQRSAAYL
                     ALNPNGLIPVLVDGAATDAAGRPLVLYETAAICLHLADTHAA"
BASE COUNT          674 a         1568 c         1579 g          698 t
ORIGIN      
        1 cggggcgatg ttcgacgcgc cgccggccgt ctcgctgatg ccctggtcgt cgtcggtcgg
       61 accgtcgagc gagtcgcgcg cgtcggagat cgcgtcggcg gaggtgcgca gcgacggcgt
      121 cgccaggccc ttcgagtaga gcgtcgatcg caccaggccg gcgtgatagg cctcggcggc
      181 gaggatgccg gcggcggctt cgaggtaggt cttgttggag atcagcggcg aagcgccctt
      241 gtacgcggtg acgccgacgt cctcgaagat gaacgcgccg agcaggaagg tctcgtcgtt
      301 ggcgtagggg tcgaatgcga cgccggcgcc caccagcccc gccgcccgcg ccgcggtcga
      361 gaaggcgccg ttcggatcgg aaaagccgac atcgatctgc ggttgcgcca cggccgatgc
      421 gccgagcgcg ctgcgcagga agttgacgtg ggccagctcg tcggcggcga tctccttggc
      481 gtattgctgg acgatcggat cggtgaaggg caccgcgtgg ccggcgatga ccgcgccttg
      541 cgttcccgtt ccggtggtca tgttcgcggc caggccggtg ccgttgacgg cgtacgaata
      601 gaactgcgcc tcgaggtatt cgagattgag ggcgaagttg aggacgtcga cgtcggagac
      661 cgcgctctgc gcggtggcgt cgttcgaatt gccgccgcac gccgccagga tcgcgccgcc
      721 ggccatgccg acagcgaagc caccggtctt gtggaagaag cgacgccggt cggcgagctt
      781 gtccgctcgc ttcttgagga cgttgagggt gagtgagctg ccaatcatga tggaaccttt
      841 cggagcaatg agacgatcag aggacgccgg caacccaagg cgtgctgccg gcatggacac
      901 agaagaagcc gcggcatgtc atcccaccat tcgctcgcga tcgccttcag caacgcccta
      961 cgcacgtcgc ttccctttgg actaaaccgc tttcgccgaa ggcctattcg gatccgtgtc
     1021 gatgcaaaag aggatgcgca cattcgaacg agcgggcacg gcgtgtgcca tgtacgcgat
     1081 tcgtcgatgt gcactcgcca tgcgaggcgg ggcgagagga gcgcctctgc gcgctataac
     1141 tcccgcatgt ccgcggaatc atggaagttc gcaccggcct acgaattgcc gtgctggccg
     1201 ttcgtggcgc cgcccgacct cggttcgggg gcgcgcatcc gctatccggt cgtcatcgcc
     1261 ggcggcggcc tcgccgggct gacgctcgcg tgcgacctgg cgctgcgcgg cgtgcgtgcg
     1321 gtgctgctcg acgaagacga caccgtcggc gtccggggcg catcgtcgcg cgggatctgc
     1381 tacgcgcaaa agagcctcga gatcttcgac cgcctgggcc tttacgagcg gatccgcgac
     1441 aaggggatca cctggtcggt gggccgcacg ttctcgggtg ccgacgagat ctacgccttc
     1501 aacctcgacg acgagagcca gtccgagcaa ccgcccttca tcaatctcca gcagttctac
     1561 ctcgagtggt tcctggtcga gcgcatcctc gagatcggcg gcaccgacgt gcgctggaag
     1621 agccgggtcg ccaccgtcga gaacggggcc gacggcgtcg tcgtcgaagt gcagacgccc
     1681 gccggcgcct attcgctcga atgcgactgg ttcatcgacg cgacgggcgc gaacagccgc
     1741 atccgcgacg cgatgggcct gcagacgcac gcttcgcgcg gcaccgatcg ctggtgcatc
     1801 agcgatgtcc gcttcaaggt gccgttcgcg gtcgagcgct ggacctggat cgacgcacca
     1861 ttcaacgagg gtcgcgccgt ctggcagcac ctcatggccg acgacgtctg gcggctcgac
     1921 taccagatgg gcggcgacgc cgaccccgaa gcgatcagca agccggaggt cgcgggcgcc
     1981 cggcttcgcc agcaactcgg ccccggggtc gaattcgagt tcgtctggat cggcccgtac
     2041 ggctatcgcg accatctgct cgagacgttt cgcgtcggcc gcacgatgtt catcggcgat
     2101 gccgcgcacg tcgtctcgcc gttcggcgcg cgtggcggca acaacggcat ccaggacgcc
     2161 gccaacctcg gctggaagct cgcgctcgtg ctcgaggagc aggccgggga cgctcttctc
     2221 gacagctacc acgtcgagcg ttacgaggcg gcggccgaga acctgaaggt gacgagccgc
     2281 acgtcgcggt tcctcgcgcc gcgcagcgcc gccgagcatc gcattcgccg cgccgtgacg
     2341 gcgctggcac gacgccatga attcgcgcgg cggctgtcga acaccgggcg catggcgcag
     2401 gccaacgact attcgccgtc gccatggtcc ggcgcggggg cacgttcggt gcagaacatc
     2461 gccttcgacg acacgacgct gatgcggtgc ctgggcggcg gcacgcgatt cctcggcttc
     2521 gtcgtcgatg ccggcccggc gctcgaaggc ctcgcgcagc tcggcgcgcg ttggccggtc
     2581 gcgtttcacg ccatcgacac ggcgtctccg ctcgccgcgc atctcggtgt ctcggcgggc
     2641 gacgtcgttc tcgtgcggcc cgacgcctac gtcgcggccc ggctgcgggc agacgacccg
     2701 gtcagcgtcg aagccgcgct gcgcgcggcg ctgtcgcgtg acgaaccgac cggggcgccc
     2761 ggcgctgccg catgagcgcg gcggcttgtc gtgatcggcc cgggccgcgg aggaatgcat
     2821 gaagacgcac gccaacatcc ccgacgccga cggcttctac gaagcgtggc tgagcgcgca
     2881 cgaaggcctg agcgacgagc agagcaccga cctcgacgcg cggctggtgc tgctgctcgc
     2941 caaccaggtc ggcgaccagg cggtgctgct cgactgcatc gccgaggcgc gccgcacggc
     3001 ggtgccgcct gcggccctca gcgtcgcaaa gccgacttga ggacgcgcac gtagttgagc
     3061 gcggccacct tctcgaccgt tgcggccggc agcttcatcg cctgcagcgc atcgatcacc
     3121 gcgcgcacct gcgcgtagtc gcttaccgac tcgctgtcgc cgacgccttc caggtcggtg
     3181 ccgatgccga cgtggtcggc gcccagccag tcgacgaggt gcgcgagctc gcgcgcatac
     3241 cccgacgcgt cgtcgcgtcc gaccgtccag ttcccctcgc cgggcgtccg gctcggcccc
     3301 ggtcgacgca ggcccagccc ccacaggccg acgacgccgc cgcgatcggc gatcttcctg
     3361 gcgagatcga gcgagagacg gcggcgcagg aagccggagc ggtcctgcca gctgccttcg
     3421 gtgcgatcga cccagccgtg cgaccagacc agcggtttcg tcgcgaccga cagcgcctgc
     3481 tcgacggccg gcccgctgca atgcgcgagg tcgaccagca cgccctgctc gttgcaggcc
     3541 tcgacgagct ggtgcccggc gtcgctcagt ccgccatgcg tcggctgcac ggtctggaag
     3601 tcgccgatcg gcgtgcggat gtagtgcacg agctgcaggt ggcgaaggcc catgtcgacg
     3661 tagccggcca gcgcatcgac gcggccttcg aggaaatcgg caccctcgct cgcgaggatt
     3721 acgcccgagt cgccggccat gcaggcgtcg acgtcggcac gcgtcagcac cgcgcgcagg
     3781 ccgctgcgcg ccacgtaggc ccgcatccgg tccagccgcc tgcgaaacgc cgtcgccagc
     3841 tcacccctgg gcgggacgct tttctggacg atgccgctgg gctcggtgcg ggtccagccg
     3901 agatcggcag gcatcgccca ggcgatcagt gcgatgcgcg cggcgcgcag ctcggcggcg
     3961 aacgtcgcgc tcggcagcgt cggccgccgc aggatgccgt agtggctgtg catgtcggcg
     4021 atcggcaccg gcggcgtcgc gccgcgcgca aggagcggtc cgaggccgaa ggccggccac
     4081 gccgcctgca gcaaggtgcg ccgcctcagg cggggcaccg gtgacgccgc gaggcaggcc
     4141 tccgcggtcg ggccggccga ttccgcaatc tccttgtccg acgctgaacc ggcttcgctg
     4201 gtcaatgcaa tccctggtgg ctcacgacga gaatgatgcc ctttcgggag cgcctggccg
     4261 atgttgcagc ttcactactt tcccggcaat gccagcctca ttgcccacgt cgtgctcgag
     4321 gagctcggcg tgccgttcga gctgaaatac gtcgatcgtg cggtgtcggc acagcgctcg
     4381 gccgcgtatc tggcgctcaa cccgaacggc ctgatcccgg tgctcgtcga cggcgcggcg
     4441 accgatgcgg ccggccgtcc gctcgtcctc tacgaaaccg cggcgatctg cctgcacctg
     4501 gccgacacgc atgcggccg
//
LOCUS       DASRWS010000433         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2452677, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000433 DASRWS010000000
VERSION     DASRWS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452677"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VFF72_08195"
     CDS             <1..1857
                     /locus_tag="VFF72_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HZV93183.1"
                     /translation="RKVLAYLREDDNQTILCVGNVSRTPQAVELELSEFAGRVPIELS
                     GASLFPPIGQLSYLLTLPPFGFYWFILAQESEAPSWHTPAPEPLPDYVTLVVLREGLA
                     AALGQSANALLERDVLPAYLAKRRWFSAKDQKISSARIAYTASIPDGDRELLMSETET
                     QIDGVTNRWLLPLSMQWDDETAGPLPGQLAVARVRRGRRVGLLTDAFAIPSFATTIAN
                     AMAEGKEIDSPEGKIIFSATEAGREVLQAKPEATVSWLSAEQSNSSLTISDTAILKLF
                     RRVSAGQHPEAEMSRYLTQQGFANTPAMLGEMVRIDDQGERHSLAVAQAFVRNQGDAW
                     TWTLNQFKRAVDDLAMNEASTEARADHVEDYQAIVASIGRQLATMHVLLARETDNKAF
                     APRVSTAEDVERWITRAHALVNKAYDCIARLPKGENESDDAAAAELVAHKGELMSAME
                     RLARSGEGTLITRIHGDFHLGQVLVATGDAFIIDFEGEPGRPLAERRAKMSPLQDVAG
                     LMRSLDYAVATTLDPRTPTYAPLPEATRVKFMRRLRDASQQAFYEAYSAGVHELQGGL
                     DNPDLLLFFMIEKAAYEVVYEAANRPAWISVPLHGLHRYTVRVLAKEVEAAS"
     gene            1854..>2054
                     /locus_tag="VFF72_08200"
     CDS             1854..>2054
                     /locus_tag="VFF72_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93184.1"
                     /translation="MSATTFALPREAVEALAGARHSNPFQVLGPHETDKGRLIRTFLP
                     GAVGVDVLRRADGRPIGELRQQN"
BASE COUNT          380 a          672 c          633 g          369 t
ORIGIN      
        1 cgtaaggtgc tggcttacct gcgcgaggac gacaaccaga cgatcctttg tgtcggcaac
       61 gtatcgcgta caccacaggc cgtggaactc gagctctcgg agttcgccgg ccgtgtgccg
      121 atcgagctta gcggcgcgtc gctcttccct ccgattggtc aactctccta cctgttgacc
      181 ctaccgccct tcggcttcta ctggttcatc ctggcccagg agagcgaggc tccgtcgtgg
      241 catacgccgg ccccagagcc gctacccgac tacgtgacgc tagtggttct acgcgagggc
      301 ctcgctgccg ccctcggaca gtcggcgaat gcgctcctgg aacgcgatgt gctgccggcc
      361 tatctcgcca agcgccgctg gttctccgcc aaggaccaga agatctcgtc ggcacgaatt
      421 gcctataccg catccatccc tgatggcgat cgagagctcc tgatgtccga gacggagacg
      481 cagatcgacg gcgtcaccaa ccgctggctg ctgccgctct ccatgcagtg ggacgacgaa
      541 acagcgggtc ccctgccggg gcagctcgcg gttgctcgcg tgcgacgagg tcgtcgggtc
      601 ggtctgttga ccgacgcctt tgcgatccca agtttcgcca cgaccatcgc gaacgcgatg
      661 gccgaaggca aggagatcga ttcgccggaa ggcaagatta tcttctccgc gacggaggcg
      721 ggccgcgagg tcttgcaggc caagccggag gccactgtaa gctggctgtc ggccgagcaa
      781 tccaacagct cgctcaccat ttcagacacg gcgatcctga agcttttccg ccgtgtctcg
      841 gccggccagc atcccgaagc cgagatgagc cgctatctca cgcagcaggg ctttgccaac
      901 acgccggcca tgctcggcga gatggtgcgg atcgacgacc agggcgaacg tcattcgctg
      961 gctgtcgctc aggccttcgt gcgtaaccaa ggtgacgcct ggacctggac gctcaatcaa
     1021 ttcaagcgtg cagtcgatga tctcgcgatg aatgaagctt cgacggaggc ccgtgcagat
     1081 cacgtcgaag actatcaagc cattgtcgcg agcattggcc gccagctcgc caccatgcat
     1141 gtcctgcttg cccgcgagac cgacaacaag gccttcgcgc cgcgcgtcag cacggccgag
     1201 gacgtcgagc gctggattac tcgcgcccat gcgctggtga acaaagcgta cgactgcatc
     1261 gcccgactgc caaaaggtga aaacgaatcg gacgatgccg cggcagcgga actcgtagcc
     1321 cacaaaggag aattgatgtc ggcgatggaa cggctcgctc gatccggcga gggcacgctg
     1381 atcacgcgca ttcatggcga tttccatctt ggccaggtgc tggttgcgac cggcgacgcc
     1441 ttcatcatcg atttcgaagg tgagccgggc cggccacttg ccgaacggag ggccaagatg
     1501 agccccttgc aggatgtggc gggcctgatg cgctcactcg actatgcggt ggcaacgacg
     1561 ctcgacccca ggacaccgac ctatgcaccg cttcccgagg caacgcgcgt caagttcatg
     1621 cggcgtctgc gagatgcctc gcagcaagcc ttctacgagg cgtacagcgc cggcgtccac
     1681 gagctgcagg gtggtctgga caatcccgat ttgttgcttt tcttcatgat cgagaaggca
     1741 gcgtacgagg tcgtctacga agccgccaac cggcccgcct ggatctccgt cccattgcat
     1801 ggactgcatc gctacacggt gcgcgtcctt gccaaagaag tggaggccgc ttcgtgagcg
     1861 cgacaacctt cgccctgccg cgcgaggccg tcgaggctct ggccggcgcc cggcacagca
     1921 atcccttcca ggtgcttggc ccgcacgaga cggacaaggg ccgcctgatc cgcacctttt
     1981 tgccgggtgc ggtcggcgtg gacgtcttgc ggcgcgctga cggccggcca atcggcgaac
     2041 ttcgccagca gaac
//
LOCUS       DASRWS010000434         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3061498, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000434 DASRWS010000000
VERSION     DASRWS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061498"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFF72_08205"
     CDS             <1..1254
                     /locus_tag="VFF72_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HZV93185.1"
                     /translation="TGRFIIFPGYEWSGNTGLGGDRNVLFLEEGRQIHRSSHALIDDL
                     SDLDTDANSAADLFDALKDENCLVFAHIGGRYADIRMAHDVRLEHAVEVHSDWGTFEW
                     LIADAFEQGYRVGIVANSDGHKGRHGASHPGASLFGAYGGLTCMLATGLSRGEIADAL
                     RARHHYATTGCRMILGVEARFAHDADRFAEDPNLGSRRSTPVRSAMMGDILRSADRSV
                     TLAIEVGGSSPIERVEIRNRLETLETFRPYGEKDLGRRIRVIWEGSEYRGRGRQTVWD
                     GSCTLSGNAFERIRPINAWNLDKKLEQVGPGQLRWAALTTGGFGGFDAWLDDAQGGTL
                     AIDTALVKCEIPVASIGLDDLVFDAGGIGRRIRVFRLPDENAQRHVEIERDIVLGDLH
                     DNALYACVTQEDGHLVWSSPIYIFR"
     gene            complement(1337..2059)
                     /locus_tag="VFF72_08210"
     CDS             complement(1337..2059)
                     /locus_tag="VFF72_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZV93186.1"
                     /translation="MNGVLTLGAALLLGLAASGHCLVMCGGISSALGVATGRGANGRP
                     RPRLLLGYQLGRVASYTLAGLVLGGVFGGVIGLLDADNVRRALRVLAAAALLLGALVA
                     FGRVRETGFGVGRRLWSHIAPLGRRLLPVTTLLRSIAFGMVWGFMPCGFVYTVLLIAT
                     VQLDAARSALTMAAFGAGTLPAMWLAGFGAQRFAGFSARPSARRVAGIALLASAALTL
                     AAPWLPEASWLHAWLPFVCTTR"
     gene            complement(2056..2220)
                     /gene="ccoS"
                     /locus_tag="VFF72_08215"
     CDS             complement(2056..2220)
                     /gene="ccoS"
                     /locus_tag="VFF72_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HZV93187.1"
                     /translation="MNILVVMIPLSIVLLIGAGIAFFWAVENDQFDDMETPGLLPLLD
                     APPAAQDGHE"
     gene            complement(2217..>3919)
                     /locus_tag="VFF72_08220"
     CDS             complement(2217..>3919)
                     /locus_tag="VFF72_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZV93188.1"
                     /translation="GMDVPVAIAIALIYAASLVQALRGSGEVYFDSVSMFVFLLLAGR
                     YLELRARHRAGHLADAMARLAPAFADRYRPDGTLERIGAHELSPGDRVHVAQGMRVPA
                     DGALDSADCRVDESLVSGESLPLRKSRGDAMTGGSMVVEGPAILVVERVGADTVLAGI
                     AALVARAQSERPRLTRAGERAAAGFVARTLALAALCAVGWSFVDPARAFAATLAVLVV
                     SCPCAFALAVPAAITRALAVLAGQGVFVVQTDAVENLALASHAVFDKTGTLTDAHPEL
                     VAVEAADRDRALGLAAALARGSRHPVAQALVRAASGLVVETASAVRTVEGGGLAGTVD
                     GSAYRLGSPAFALDAEGSDDDDVVILAGEHGVVATFRLNERLRPGAREAVAALVASGV
                     EVEIVSGDAASKVADVARRLGIAQWRARQSPASKLERLRELRAGGAHVLAIGDGINDA
                     PVLAGADVAVALASGAELAQAAGDVILDGDNLAALPAARAIARETLAVLRQNQRWALL
                     YNLAVVPLAALGLVAPWLAALGMSASSLGVVLNALRIGRGAPAPASVARSGTESRRWA
                     "
BASE COUNT          622 a         1412 c         1413 g          472 t
ORIGIN      
        1 acagggcgct tcatcatctt cccgggctac gaatggtcgg gcaacaccgg cctcggcggc
       61 gaccgcaacg tgctcttcct cgaagaaggg cggcagatcc accgttcatc gcatgcgctg
      121 atcgacgacc tttccgacct cgacaccgac gccaactcgg cagccgacct gttcgacgcg
      181 ctgaaggacg agaactgcct cgtcttcgcc cacatcggcg gccgctacgc cgacatcagg
      241 atggcgcacg acgtgcgtct cgagcacgcg gtcgaggtgc attcggactg gggcaccttc
      301 gaatggctga tcgccgatgc gttcgagcag ggctatcgcg tcggcatcgt cgccaacagc
      361 gacggccaca agggccggca tggtgcgagc catccgggcg cgtcgctgtt cggtgcctac
      421 ggcggcctca cctgcatgct ggcgacgggg ctcagccgcg gcgaaatcgc cgatgcgttg
      481 cgggcgcggc atcactacgc gacgaccggc tgccggatga tcctcggcgt cgaggcgcgc
      541 ttcgcgcatg atgccgatcg cttcgccgaa gacccgaacc tcggctcgcg gcggtcgacg
      601 ccggtgcgca gcgcgatgat gggcgacatc ctgaggagcg ccgaccggtc ggtgacgctc
      661 gccatcgagg tgggcggttc gagcccgatc gagcgcgtcg agatccgcaa ccgcctcgag
      721 acgctcgaaa cctttcgacc ctatggcgag aaggatctgg ggcgccgcat ccgcgtgatc
      781 tgggagggct ccgaataccg ggggcgcggc cggcagaccg tctgggacgg cagctgcacg
      841 ctgagcggca acgccttcga gcgcatccgc ccgatcaacg cctggaatct cgacaagaag
      901 ctcgagcagg tcggccccgg ccaactccgc tgggcggcgc tcaccacggg cggcttcggc
      961 gggttcgatg cgtggctcga cgacgcgcag ggcggaacgc tggcgatcga cacggcgctg
     1021 gtgaagtgcg agattccggt ggcctcgatc ggcctcgacg acctcgtctt cgatgccggc
     1081 ggcatcgggc gacgcatccg ggtgttccgc ctgccggacg agaacgcgca acggcacgtc
     1141 gagatcgaac gcgacatcgt tctcggcgac ctgcacgaca acgcgctcta tgcgtgcgtc
     1201 acgcaggaag acggccacct ggtctggtcg agtccgatct acatcttccg ctgatcgctc
     1261 gcgaccgatc gacgcgaccg atcgacgcgt tgatcggcgc gaagattctg cgccggcgcc
     1321 ccaacgctgc cgaccctcag cgcgtcgtgc agacgaacgg caaccacgca tgcagccagg
     1381 acgcttccgg cagccagggg gcggcgaggg tcagcgcggc actggccagc aaggcgatgc
     1441 cggcgacgcg ccgcgccgaa ggccgggcgc tgaatccggc gaagcgctgg gcgccgaaac
     1501 cggcgagcca catcgccggc agcgtgcccg cgccgaaggc ggccatcgtc agcgcgcttc
     1561 gcgcggcatc gagttgaacc gtggcgatca gcagcacggt gtagacgaag ccgcacggca
     1621 tgaagcccca gaccatgccg aaagcgatcg agcgcagcag cgtcgtcacg ggcaggaggc
     1681 ggcgcccgag cggggcgatg tgcgaccaga ggcgtcggcc gacgccgaag ccggtctcgc
     1741 gcacacggcc gaacgcgacc agcgcgccga gcagcagggc ggccgcggcg agaacgcgca
     1801 gggcgcggcg gacgttgtcg gcatcgagca ggccgatcac gccaccgaac acgccgccga
     1861 gcacgagacc ggccagcgtg tacgaggcga cacggccgag ctggtagccc agcagcagcc
     1921 gcggccgtgg ccggccgttg gcgccgcggc cggtggcgac gccgagtgcc gaggagatgc
     1981 cgccgcacat gaccaggcag tggccgctgg cggccaggcc gagcagcagc gcggcgccga
     2041 gcgtcaggac gccgttcatt cgtgcccgtc ttgggccgcg ggtggcgcgt cgagcagcgg
     2101 cagcagcccc ggcgtctcca tgtcgtcgaa ctggtcgttc tcgacggccc agaagaaggc
     2161 gatgccggcg ccgatcagga gcacgatcga cagcgggatc atcacgacga ggatgttcat
     2221 gcccagcgcc tcgactcggt tccgctgcgg gcgacacttg ccggcgccgg ggcgccgcgc
     2281 ccgatccgca gcgcgttgag cacgaccccg agcgagctcg ccgacatgcc gagcgcggcc
     2341 agccacggcg ccacgaggcc gagcgccgcg agcggcacga cggcgaggtt gtagagcagt
     2401 gcccagcgct ggttctggcg cagcacggcc agggtctcgc gcgcgatcgc gcgcgctgcc
     2461 ggcagcgcgg ccaggttgtc gccatcgagg atcacgtcgc cggcggcctg cgccagctcg
     2521 gcgcccgacg ccagcgccac cgcgacatcg gcgcccgcca gcacgggcgc gtcgttgatg
     2581 ccgtcgccga tcgccagcac gtgggcgccg ccggcgcgca gctcgcgcag ccgctcgagc
     2641 ttcgaggccg ggctctggcg cgcccgccac tgcgcgatgc ccaggcgacg ggccacgtcg
     2701 gcgactttcg acgccgcgtc gccgctgacg atctcgacct cgacgccgct ggcgaccagc
     2761 gcggccacgg cctcgcgcgc gccggggcga agccgctcgt tcaggcgaaa cgtggcgacg
     2821 acgccgtgct cacctgcgag gatcacgacg tcgtcatcgt cgctgccctc ggcgtcgagg
     2881 gcgaacgccg ggctgcccaa gcggtacgcg gagccgtcga ccgtgcccgc gagtccgcca
     2941 ccttcgaccg tgcggacggc actcgccgtc tccacaacca gccccgacgc cgcacgcacc
     3001 agggcctgtg ccaccgggtg gcggctgccg cgcgccagcg ccgcggccaa cccgagcgca
     3061 cgatcacggt cggccgcctc gacggcgacg agctccggat gcgcgtcggt cagcgtgccg
     3121 gtcttgtcga acaccgcgtg gctggccagc gccaggttct cgaccgcatc ggtttggacg
     3181 acgaacacgc cttgcccggc cagcacggcc agcgcccgcg tgatcgcggc cggcaccgcg
     3241 agcgcgaacg cgcacgggca cgacaccacc aggacggcga gcgtcgccgc gaaggcgcgc
     3301 gccggatcga cgaagctcca gccgacagcg cacagcgccg ccagggccag cgtgcgcgcg
     3361 acgaagccgg cggccgcgcg ttcgcccgct cgcgtcagcc gggggcgttc gctctgggcg
     3421 cgcgcgacca gcgccgcgat gccggcgagc accgtgtcgg cgcccactcg ctcgacgacg
     3481 aggatcgccg ggccttcgac caccatgctg ccgccggtca tggcgtcgcc gcggctttta
     3541 cgcagcggca gggactcgcc cgagacaagc gactcgtcga cccggcagtc ggcgctgtcg
     3601 agcgcgccgt cggcgggcac gcgcatgccc tgggcgacgt ggacgcggtc gccgggggag
     3661 agctcgtgag cgccgatacg ttcgagcgtg ccgtcggggc ggtagcggtc ggcgaaggcg
     3721 ggcgcgagcc tggccatggc gtcggccaga tgcccggcgc gatgacgtgc acgcagctcg
     3781 aggtagcggc cggcgagcag gaggaagacg aacatcgaca ccgagtcgaa atacacctcg
     3841 ccgctgccac gcagcgcctg caccaggctg gcggcataga tcagcgcgat ggcgatcgcg
     3901 accggcacgt ccatgccga
//
LOCUS       DASRWS010000435         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4204139, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000435 DASRWS010000000
VERSION     DASRWS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204139"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFF72_08225"
     CDS             complement(<1..462)
                     /locus_tag="VFF72_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZV93189.1"
                     /translation="MSSTRADSRRRRLLVGALALGTVLLLAAGIAAGSDGLSWADLVQ
                     TWHATDARLIIGEIRAPRTVGAWLTGALLGLAGAVAQGLFRNPLADPYLLGSASGATL
                     GVVLILAAKPLAGNAISLAAADAVARFGLVGAAFVGALLGVLLTLALARGAQ"
     gene            complement(459..1370)
                     /locus_tag="VFF72_08230"
     CDS             complement(459..1370)
                     /locus_tag="VFF72_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HZV93190.1"
                     /translation="MTKAATRLSAPARHVVASLLAIVLAAASAGAQAGIVLRDDRGID
                     VTLPSVPRRVVSLLPSITECVCAVGACARLVGTDRFSDWPASVLALPRLGGLDDAQIE
                     RIVALRPDVVLLSSSARVIDRLENLGLKVVVLESRNRADLEHTLTLLGKMFEDPAAAA
                     SVWSRIEAQTRAAAKRIPAGLRGERVYFEIDSTPYAAGPTSFVGELLSSLGMGNIVAP
                     GLGPFPKLNPEYVVRAQPDIVMAADEELRGMAERPGWSSLHALRRRKACGFGLAKYEL
                     LVRPGPRMGEAAGVLADCLVSIAAAGR"
     gene            complement(1388..>2512)
                     /locus_tag="VFF72_08235"
     CDS             complement(1388..>2512)
                     /locus_tag="VFF72_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZV93191.1"
                     /translation="NAQYDSAEFNPPDYTADPSPDFRNRLHTSVVSLDYRGVVSPLWT
                     TTLQASRNVDDLNSGGTTLTRYKTDREQQTWQNALHLGAGQQVMLAYEHLGEKVSGDV
                     FTAEPQRTNNAFVLGYSGRYGLAGLQASVRHDDNSVYGDNTTGSVGATYQISPQFKLR
                     ALAGTTFRAPTFNDLFYPGYGVSSIQPERGRSFEVGASWQSGNTSATATVYRNDVRDL
                     IGYDPDPTGAHCPPGYFGCAANTSRARLQGATLGATQQWDGLEMRATVDFLDARDADT
                     GVRLARRAAHQETLAVSYTRNAWSAGASLLDVGSRPDGGVVLGGYGIVDVHAAWRFHA
                     RWRLEAKLLNALDHRVEPVRDYQGLGRQAWIGVTFDGNGF"
BASE COUNT          387 a          861 c          907 g          357 t
ORIGIN      
        1 ctgggcgccc cgcgccagcg ccagcgtgag caggacgccg agcagcgcgc cgacgaacgc
       61 cgcgccgacg agcccgaagc gcgcgaccgc gtctgcggcc gccaggctga tcgcgttgcc
      121 agcgaggggt ttcgccgcga ggatcagcac gacgcccagc gtcgcacccg acgccgagcc
      181 gagaagatag gggtcggcca ggggattgcg gaacagcccc tgcgcgaccg ctccggccag
      241 cccgagcagt gcgcctgtca gccatgcgcc caccgtccgc ggcgcgcgga tctcgccgat
      301 gatgagtcgc gcgtcggtgg cgtgccatgt ctggacgaga tccgcccacg acaggccatc
      361 gctgcccgcg gcgatgcctg ccgccagcag caggaccgtg ccgagcgcga gcgcgccgac
      421 gagcagccgc cgtcgccggg aatcggctcg cgtcgagctc atcggcctgc cgccgcgatc
      481 gagacgaggc agtcggcgag cacgccggcc gcctcgccca tgcgcggtcc gggccggacc
      541 agcagctcgt acttcgcgag gccgaagccg caggcctttc gacgtcgcag cgcgtggagg
      601 gacgaccacc ccggccgctc ggccatgcct cggagctcct cgtcggccgc catgacgatg
      661 tcgggctgag cacggacgac gtactccgga ttgagcttcg ggaagggccc gaggccgggc
      721 gcgacgatgt tgcccatgcc caggctcgac agcagttcgc cgacgaacga tgtcgggccg
      781 gcggcatacg gtgtcgagtc gatctcgaag tagacccgct cgccgcgcaa cccggccgga
      841 atccgcttgg ccgcggcacg ggtttgcgct tcgattcgcg accacaccga ggccgccgcc
      901 gccggatcct cgaacatctt gccgaggagc gtcagcgtgt gctcgaggtc ggcgcgattg
      961 cgcgattcca ggacgacgac cttgaggccg aggttctcga gtcggtcgat gacccgcgcc
     1021 gaagacgaca gcaagacgac gtcgggccgc agtgccacga tgcgttcgat ctgcgcgtcg
     1081 tcgaggccgc ccagcctggg caaggcgaga acgctcgccg gccagtcgga gaatcgatcg
     1141 gtgccgacga gtcgcgcgca ggcgccgacc gcgcagacgc attcggtgat cgacggcagc
     1201 aggctgacga cccggcgcgg cacgctgggg agcgtcacgt cgatgccgcg gtcgtcgcgc
     1261 aggacgatcc cggcctgcgc gccggccgat gcggccgcca gcacgatcgc gagcaacgag
     1321 gcgacgacgt gtcgcgccgg cgcgctgagg cgggttgcgg ccttggtcat ggcaccgaac
     1381 gcctgaatca gaacccgttg ccgtcgaagg tcacgccgat ccatgcctgg cggcccagac
     1441 cctggtagtc gcgcaccggc tcgacccgat gatcgagcgc gttcagcagc ttcgcttcga
     1501 ggcgccagcg cgcgtggaag cgccaagcgg cgtgcacgtc gacgatgccg tacccgccga
     1561 gaacgacgcc gccatcggga cgcgagccga cgtcgagcaa cgacgcccca gcgctccacg
     1621 cgttgcgcgt gtagctgacg gcgagcgtct cctggtgcgc cgcgcgacga gccaggcgca
     1681 cgccggtatc ggcatccctg gcgtcgagga aatcgacggt cgctcgcatt tccaggccgt
     1741 cccactgctg cgttgcaccg agcgtcgcac cttgcaaccg ggcccgcgac gtgttcgctg
     1801 cgcagccgaa ataccctggc gggcaatgcg cgccggtggg atcggggtcg tagccgatca
     1861 ggtcgcgaac gtcgttgcga tagacggtgg cggtcgcgct cgtgttgccg gattgccagg
     1921 aggcgcccac ctcgaagctg cggccccgct ccggctggat cgacgagacg ccgtagccgg
     1981 ggtagaagag gtcgttgaag gtcggcgcgc ggaacgtcgt tccggccagc gcccgcagct
     2041 tgaactgcgg cgagatttgg tacgtcgcgc cgacgctgcc ggtcgtgttg tcgccgtaga
     2101 cggagttgtc gtcgtgccgg acgctcgcct gcaggccggc gagtccgtac cggccggagt
     2161 agccgagcac gaaggcgttg ttggtgcgtt gcggctcggc ggtgaagacg tcgcccgaga
     2221 ccttctcgcc aagatgctcg taggcgagca tcacctgctg gccggcgccg aggtgcaacg
     2281 cgttctgcca ggtctgctgc tcgcggtcgg tcttgtagcg ggtcagcgtc gtgccgccgc
     2341 tgttcaggtc gtcgacgttc ctcgacgcct gcagcgtcgt cgtccacagt ggggacacca
     2401 cgccgcggta gtcgagagac acgaccgagg tgtgcagacg attgcgaaag tcgggcgaag
     2461 ggtcggcggt gtagtcgggc ggattgaatt ccgcactgtc gtactgcgcg tt
//
LOCUS       DASRWS010000436         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3915246, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000436 DASRWS010000000
VERSION     DASRWS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3915246"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFF72_08240"
     CDS             <1..286
                     /locus_tag="VFF72_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93192.1"
                     /translation="PITGMATATNAFTIGGTILPKPLINGTATQLGMRGDPNSVTIDP
                     GRNYAYMLADTQPSYHGFNVNYPLFLIRVDLNGASVNTPWNPTMQSIAMP"
     gene            complement(326..1165)
                     /locus_tag="VFF72_08245"
     CDS             complement(326..1165)
                     /locus_tag="VFF72_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZV93193.1"
                     /translation="MTQHSEFFELSQDDNGIAHLRLNRPERMNTMTLAFFPELRDAVR
                     RLADDGATRVLVISSTGKHFCAGMSLDVFAAEPAALGVATARQRLAFQESLRRLMACF
                     TALDEAPFPSLCAIQGGCIGGALDLATACDIRLCSADAFFTVQEITIGMVADLGTMQR
                     LPKIVPQGVAREMAYSGERMGADRALAVGLVNAVLSDPAALLERAMALAKTIASKSPL
                     AIAGSKLALNHARDHSTASALHQMTLLQSAIFDSGEIETAIAGWKEKRAAAFDALARI
                     PEI"
     gene            1183..2046
                     /locus_tag="VFF72_08250"
     CDS             1183..2046
                     /locus_tag="VFF72_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV93194.1"
                     /translation="MDESIETADGLTLRGREWPLAGAKGTIVLVHGLGEHVGRHAHVA
                     AFLNGCGWRVVGFDHRGHGTSDGGRGRLRRDDDLLSDLAGVVDLVRRNFPGPLVLLGH
                     SLGGLVAARFVAEGVRESGLAAWSRPVDALVLSSPVLATGMSLAQKAMLAVLGPLAPN
                     LAVGNGLKPEWISRDSAVVAAYRADPLVHDRIAPRLARFIVDGGEFVREAAPRWNVPT
                     LLLYAGADRCVEPSGSAAFAAAAPRGMVTTRAFTPLSHEIFNEPERAEVFSVLGDWLD
                     TLPLSIFRSPR"
     gene            2043..3536
                     /locus_tag="VFF72_08255"
     CDS             2043..3536
                     /locus_tag="VFF72_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZV93195.1"
                     /translation="MNARDPLLPVQADEAEALGRFADRVWDDEIVPAITRYIEVPAKS
                     PMFDAEWKTHGFIDHVVRDAAAWVERQQVAGLKLEVISLEGRTPIIFFDVPATRPGGN
                     EADAPTVCLYGHLDKQPEFNGWKNGFGPWTPRYEGGRLYGRGGADDGYAIYAAVTAIQ
                     ALDKQGIARPRCVGIVETCEESGSPDLPFYVDALRPRLGQVELVVCLDSGAGNYDQLW
                     LTTSLRGMVSGVLKVEILGEGVHSGDASGLVPSSFRVLRQVLDRLEDSKSGRLLPASL
                     HCEIPADRLEQARATAKILGDEVWKRFPWACGADGGPTLPTTTDPVEALVNRTWRPTL
                     SVTGVEGFPALKDAGNVLRPYTAFKLSLRLPPLVDGNEAAQTVKKLLEDNAPYNARVT
                     FHPDGRAGAAGATGWNAPSLAPWLQASLDAASNAHFGAPCGYIGQGGTIPLMSMLQKS
                     FPASQMMVCGVLGPKSNAHGPNEFLHVPYGKKLTAAVAQVMAALPGR"
BASE COUNT          621 a         1270 c         1284 g          608 t
ORIGIN      
        1 gccgatcacc ggcatggcca ccgcgaccaa cgccttcacg atcggcggca ccatcctgcc
       61 caagccgctg atcaacggaa cggccaccca gctcgggatg cgtggcgacc cgaactcggt
      121 gacgatcgat ccggggcgca actacgccta tatgctggcc gacacgcagc cgagctacca
      181 tgggttcaac gtgaactacc cgctgttcct gatccgcgtc gatctgaacg gggcaagcgt
      241 gaacacgccg tggaatccga cgatgcagtc gatcgcgatg ccctgatcga aggcatccgc
      301 cacgaaggcc gggtccgctc ggccttcaga tttccggaat ccgggccaag gcgtcgaacg
      361 ccgcggcccg tttttccttc cagcctgcga tcgcggtctc gatctcgcca ctgtcgaaga
      421 tcgcgctttg cagcagcgtc atctggtgca atgccgaagc ggtcgagtga tcgcgggcgt
      481 gattgagggc gagcttgctg ccggcgatgg ccagcggcga cttcgacgcg atggttttcg
      541 ccagtgccat cgcccgctcg agcaacgctg ccggatcgga tagcacggca ttgaccagcc
      601 cgacggcgag ggcgcggtcg gcgcccatcc tctcgccgct gtaggccatc tcgcgggcga
      661 cgccttgcgg cacgatcttg ggcaggcgct gcatcgttcc gaggtcggcg accatgccga
      721 tcgtgatctc ctggacggtg aagaacgcat ccgcactgca caggcggata tcgcaagcgg
      781 tcgccaggtc gagggcgccg ccgatgcagc caccctggat cgcgcacaga cttggaaagg
      841 gcgcctcgtc gagggcggtg aagcaggcca tcaggcgacg caatgactcc tggaaggcaa
      901 gtcgttggcg agcagtcgcc acgccgagcg cggccggttc ggcggcgaag acgtcgaggg
      961 acatgccggc gcaaaagtgc ttgccggtcg aggaaatgac gagcacgcgt gtcgcaccgt
     1021 cgtcggcgag ccggcgtacg gcgtcacgca gctcggggaa gaaggcgagc gtcatcgtgt
     1081 tcatgcgctc cggacgattg agtcgcaggt gcgcgatgcc gttatcgtcc tgggagagtt
     1141 cgaagaattc gctgtgctgg gtcatggcgt tggaggctgc tggtggacga gagcatcgaa
     1201 accgcggacg ggctcacgtt gcgcggccgc gagtggccgc tggccggcgc gaaggggacg
     1261 attgtcctcg tccacggcct gggcgaacac gtcggacgcc atgcgcacgt cgccgcattc
     1321 ctcaacgggt gcggctggcg ggtcgtcggc ttcgatcatc gcggccacgg cacgagcgac
     1381 ggcggccgcg gacggctgcg ccgcgatgac gacctgcttt ccgatctggc cggagtcgtc
     1441 gatctcgtcc gtcgcaactt tcccggcccg ctggtgctgc tcgggcacag cctgggaggg
     1501 ctggtcgcgg cgcgtttcgt cgcagaaggc gtgcgcgaaa gcggcctggc ggcgtggtcg
     1561 cggccggtcg atgcgctcgt cctgtcttcg ccggtgcttg ccaccggcat gagcctggcg
     1621 cagaaggcga tgctcgcggt gctgggcccg ctcgcgccga acctcgcggt cggcaacggt
     1681 ttgaagccgg aatggatctc tcgcgactct gccgtcgtgg cggcgtaccg cgccgacccg
     1741 ctcgtccacg accgcatcgc gccccgcctg gcgcgcttca tcgtcgacgg cggtgaattc
     1801 gtgcgcgagg ccgcgccgcg ctggaacgtg ccgacgcttc tcctctacgc cggcgccgac
     1861 cgctgcgtcg agccatcggg cagcgcggcc ttcgcggcgg cggcaccgag aggcatggtg
     1921 acgacacgcg ccttcacgcc gctctctcac gagatcttca acgaacccga gcgggccgaa
     1981 gtgttttcgg tgctcggcga ctggctcgat acactgcctc tttcgatatt ccggagcccg
     2041 cgatgaatgc acgcgatccc ctcctgccgg ttcaggccga cgaagccgaa gcgctcggcc
     2101 gattcgccga ccgtgtctgg gacgacgaaa tcgttccagc catcacccga tacatcgagg
     2161 tgccggcgaa gagccccatg ttcgacgccg agtggaagac gcatggcttc atcgaccacg
     2221 tggtgcgcga cgcggcagcc tgggtcgagc gccagcaggt cgccgggctc aagctcgagg
     2281 tcatcagcct cgagggtcgc acgccgatca tcttcttcga cgtgccggcg acgcgaccgg
     2341 gcggcaacga agccgatgcg cctactgtct gcctctacgg ccatctcgac aagcagcccg
     2401 agttcaacgg ctggaagaac ggcttcggac cgtggacccc acgctacgaa ggcggtcggc
     2461 tctatggccg cggcggcgcc gacgacggct acgcgatcta cgcggccgtc accgcgatcc
     2521 aggcgctcga caagcaggga atcgcccggc cccgctgcgt cggcatcgtc gagacgtgcg
     2581 aggaaagcgg ctctcccgac ctgccgttct atgtcgacgc gctgcgcccg cggctcggcc
     2641 aggtcgagct cgtcgtctgt ctcgacagcg gcgccggcaa ctacgaccag ctctggctca
     2701 cgaccagcct gcgcggcatg gtgagcggcg tgctcaaggt cgagatcctc ggcgagggcg
     2761 tgcattccgg cgacgcgagc gggctcgtgc cgtcgagctt ccgggtcctg cgccaggtgc
     2821 tcgaccggct cgaggattcg aagagcgggc gtctgctgcc tgcgagcctg cactgcgaga
     2881 ttccggccga tcgccttgaa caagccaggg ccaccgcgaa gatcctcggc gacgaggtct
     2941 ggaagcgctt cccgtgggct tgcggcgccg acgggggacc gaccctgccg acgacgaccg
     3001 atccggtcga agcactcgtc aatcgcacgt ggaggccgac tctgtcggtc accggcgtcg
     3061 agggtttccc cgcgctgaag gatgccggca acgtgctgcg tccgtacacg gcattcaagc
     3121 tcagcctgcg cctgccgccg ctcgtcgacg gcaacgaggc ggcgcagacg gtgaagaagc
     3181 tgctcgagga caacgcgccc tacaacgcca gggtgacttt ccaccccgac ggtcgtgccg
     3241 gcgccgcggg agccaccggc tggaacgcac cgagtctcgc gccttggctg caggcgtcgc
     3301 tcgacgccgc ctcgaacgct cacttcggcg cgccctgcgg ctacatcggc cagggcggga
     3361 ccatcccgct catgagcatg ttgcagaaga gttttccggc atcgcagatg atggtctgcg
     3421 gcgtcctcgg cccgaagagc aacgcgcacg ggcccaacga gttcctgcat gtgccttatg
     3481 gcaagaagtt gaccgctgca gtagcgcagg tcatggccgc cctgccgggg aggtaaaccc
     3541 cctgttcgcg gggtggcgga cccgcgagtg aagcacagaa tgcttactcg tattacgtca
     3601 ggaaaccggc cgcgatcgag ccggacacag gagtgagcaa tgaaccagtt cttcaagggc
     3661 ggcgcggcac tgggtgtcgc gttgctggcg ggccacgcga tggccgctgt cacaacgatt
     3721 cccgactcgg ccttgacacc cacgacccag ctctacacga atgacctggg cgccatcgtc
     3781 gtc
//
LOCUS       DASRWS010000437         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1038904, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000437 DASRWS010000000
VERSION     DASRWS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038904"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFF72_08260"
     CDS             <1..397
                     /locus_tag="VFF72_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV93196.1"
                     /translation="IAEFELVQQMIAESNTEAHAARALVLETARKRDAGVDVTMEASM
                     CKLFASEMCGRVADRAVQVFGGGGYIADNVAERFYRDVRLFRLYEGTSQIHLVNIARR
                     TVAARGKSSSPNAHAPPLRDHFPTDKETS"
     gene            394..1383
                     /locus_tag="VFF72_08265"
     CDS             394..1383
                     /locus_tag="VFF72_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV93197.1"
                     /translation="MKSLVTRYAVAAALALALPFTARAESAAAYPSKPIHIIVPYSAG
                     GIVDTLARIVGERLSKKYGQPVIVETKPGAGGAIGNEYVVKAAPDGYTLLCVSPGLAV
                     LPSLQKNADWTAASFEGVEGLGVISNVWVVPASSPIKTMSDLIAVAKKKAASGAPMTY
                     ATAGIGTSNYLSGELLSQMTGIKLTQVPYKGQPDALTDLLAGRVESMPLTAALAQAHV
                     KSGQLRPLAVTTKQRSSAFPELPTVAQAIDLPDYAVGTWFGFVAPAKTPPAIIEKLSK
                     DIAEIIAEPEPKKKLAALGMELDPQNAKEFNAYIAAETARWSKVIRQAGIKPQ"
     gene            1380..2486
                     /locus_tag="VFF72_08270"
     CDS             1380..2486
                     /locus_tag="VFF72_08270"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZV93198.1"
                     /translation="MKDTDAVMRTPICDRLGIAVPIFGFSHSLDVVAAIAEAGGFGVL
                     GLAREMPDDIPALIAATEDRLGDRPFGIDLMLPSHVPRAATIDEVQSRLPPESVAFVA
                     GLRSRFGVAPPTRPSFFTTQVRSEALFEAQIDAVLASRACAVATAIGLRADLFHRARA
                     RGKLTFSLVGTPRHARKALDLGVDVLVAQGYDAGGHTGPIGTFSLVPQIVALADKTPV
                     LAAGGIGNGEQIAAAIAMGAQGGWLGTLWMAACESHTPPALLRRLIASGSEDTLITRA
                     HSGKPCRVVRSDWIDAWSEPGAPAPLAMPLQQALTGDVFASIHEHDDERLVYDAAGQS
                     VFAIERATTIAEQMRELQAGMRRAQRRFKEAAVP"
     gene            complement(2705..2983)
                     /locus_tag="VFF72_08275"
     CDS             complement(2705..2983)
                     /locus_tag="VFF72_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93199.1"
                     /translation="MHAQPLSIDVSTPIFRSVSQYTLGLAIAAALVVATAELIVLQDH
                     PEAAAPPDAVALAAFGDAVFGPMRAKGTVVVSLPFGLRPIGAREHVDR"
BASE COUNT          565 a         1219 c         1221 g          541 t
ORIGIN      
        1 gatcgccgag ttcgagctcg tgcagcagat gatcgccgag agcaacaccg aggcgcatgc
       61 cgcgcgtgcg ctggtgctcg agaccgcgag gaagcgcgac gccggcgtcg acgtgacgat
      121 ggaggcgtcg atgtgcaagc tcttcgcctc cgagatgtgc ggacgcgtcg ccgatcgcgc
      181 ggtgcaggtc ttcggcggcg gcggatacat cgccgacaac gtcgcagagc gcttctaccg
      241 cgacgtgcgc ctgttccggc tctacgaggg cacgagccag atccacctcg tcaatatcgc
      301 gcgccgcacg gtcgcggcac gcggcaagtc gtcgtccccg aacgcgcacg ctcccccgct
      361 gcgagatcac ttccctaccg acaaggagac atcatgaagt cgttggtcac ccgatacgcc
      421 gtcgcggcgg cgcttgcgct ggcccttccg ttcaccgccc gagcagagtc ggccgcggcc
      481 tatccgtcga agccgatcca catcatcgtt ccgtactcgg ccggcggcat cgtcgacacg
      541 ctcgcgcgga tcgtcggcga gcgcctctcg aagaagtacg ggcagccggt gatcgtcgag
      601 accaagcccg gcgccggcgg cgcgatcggc aacgaatacg tcgtcaaggc cgcacccgac
      661 ggctacacgc tgctttgcgt gagcccgggt ctcgccgtcc tgccgagcct gcagaaaaat
      721 gccgactgga ccgccgcctc cttcgagggc gtcgaaggcc tcggggtcat ctcgaacgtc
      781 tgggtggtgc ccgcgtcgtc accgatcaag acgatgtccg acctgatcgc cgtcgcgaag
      841 aaaaaggccg cgagcggcgc gccgatgacc tatgcgacgg ccggcatcgg cacctcgaac
      901 tacctttccg gcgaactgct gtcgcagatg accggcatca agctgacgca agtgccctac
      961 aagggccagc ccgacgcgct gaccgacctg ctggccggtc gtgtcgagtc gatgccgctc
     1021 accgcggcgc tggcgcaggc gcacgtcaag tcgggtcagc tgcgcccgtt ggcggtgacg
     1081 acgaagcagc gctcgagcgc cttcccggag ttgccgaccg tcgcccaggc gatcgacctg
     1141 ccggattacg ccgtcggtac gtggttcggc ttcgtcgccc cggcgaagac gccgccggcg
     1201 atcatcgaga agctgtcgaa ggacattgcc gagatcattg ccgagccgga gccgaaaaag
     1261 aagctcgcgg ccctcggcat ggaactggat ccgcagaacg cgaaggagtt caacgcctac
     1321 atcgccgcag agacggcgcg ctggtcgaag gtgatcaggc aggctggcat caagccacag
     1381 tgaaagacac cgacgcggtg atgcgcacgc cgatctgcga ccggctcggc atcgccgtgc
     1441 cgatcttcgg gttctcgcat tcgctcgacg tcgttgccgc gatcgccgag gccggcggct
     1501 tcggcgtgct cggcctggcg cgtgagatgc ccgacgacat cccggcgctg atcgccgcca
     1561 cggaggatcg cctcggcgac cggccgttcg gcatcgacct gatgctgccg tcccatgtgc
     1621 cgcgggctgc gacgatcgac gaggtgcagt cgcgactgcc gccggaaagc gtcgcgttcg
     1681 tcgccggctt gcggagtcgt ttcggcgtcg cgccgccgac gcggccgagc tttttcacga
     1741 cgcaggtccg atccgaagcg ctcttcgagg cgcagatcga tgcggtcctg gcctcgcgcg
     1801 cttgcgcggt cgccaccgcg atcggccttc gcgccgatct cttccatcgc gcccgtgccc
     1861 gcggcaagct gacgttctcg ctggtcggca cgccgcgcca tgcacgcaag gcgctcgacc
     1921 tcggcgtcga cgtgctggtg gcgcaaggct acgacgccgg cggtcacact ggcccgatcg
     1981 gcaccttctc gctggtaccg cagatcgtcg cgctggccga caagacgccg gtgctggccg
     2041 ccggcggcat cggcaacggc gagcagatcg ccgcggcgat cgcgatggga gcgcagggtg
     2101 gctggctcgg cacgctctgg atggcggcgt gcgagagtca cacgccgccg gcgctgctga
     2161 ggcggctgat cgcgagcggc agcgaagaca cgctgatcac gcgcgcgcac agcggcaagc
     2221 cgtgccgcgt cgtgcgcagc gactggatcg atgcctggag cgagccgggc gctccggctc
     2281 cgctggcgat gccgctgcag caggcgctga ccggcgacgt cttcgcgtcg atccacgagc
     2341 acgacgacga gcgcctcgtc tacgacgcgg caggccagag cgtgttcgcg atcgagcgcg
     2401 caacgacgat cgccgagcag atgcgcgagc tgcaggcggg aatgcgacgc gcacaacgtc
     2461 gcttcaagga agcggcagtg ccctgaccgt cgcagccgat ccttcgcgaa gcggcattct
     2521 tctgctgcgc gtggcggcgc cagccagggc acgaccgcct tgacctcgac gggcgtcggc
     2581 aacatcaacg ttgaccgtcg gatcggcgag tctcacctgg cgcctgctcc gcccgtgtca
     2641 caagcggcgc cgcccgaggc gagtcgattt cgcctcgccg ccgagcccgg cgcaagccga
     2701 gcggtcaacg atcgacgtgc tcgcgtgctc cgatcggccg caggccgaag ggaaggctca
     2761 cgaccacggt gccctttgcg cgcatcggcc cgaacacggc gtcgccgaag gcggcaagcg
     2821 ccacggcatc cggcggtgcc gcggcctcgg gatggtcttg caggacgatc agctcggccg
     2881 ttgcgacgac gagggcggct gcgattgcga gtcccagcgt gtactgcgaa acgctacgga
     2941 aaatcggcgt tgaaacgtcg atcgacagcg gttgtgcgtg catcgagatc tcctttggcg
     3001 attggggcga ccctggcggg cgggtggtcg ggccgtcgaa gtggcccttg gtccttaaag
     3061 cggaattcga tggccaaaga ggacatgcgg tcacgaagtg acgccatttg tgggggcacg
     3121 tggccggcgc ggtgcattct tgccctcgac ttcggcgctc gccctggctt cggcgttggc
     3181 gtggtgcacc gcgtcgaggc acagtgccgc gcctcgagtc gatcgagaca tcaggctgtc
     3241 gacggccgac aggcgcggta acgacggccg acaggcaccg ctacgacggc ttgcagtcag
     3301 gatcgtgaca tggccgtgaa tctcgatctc gccgttgccg accgaaacgg ccaatcattc
     3361 gcgagacggc cccgtcgtca cactgtcgcg gccggtccgg tttgcagcag cgccagcgcc
     3421 gcgccttcgt cgagccgccg tccggcgtcg gccagttcgt cgaagcgctc gcgcccgagg
     3481 gccgcggccg ccgcggacgt cgcgttctcg accgtcgcca gcgccgcgcc atagagtgcc
     3541 atggcg
//
LOCUS       DASRWS010000438         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3915765, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000438 DASRWS010000000
VERSION     DASRWS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3915765"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFF72_08280"
     CDS             <1..768
                     /locus_tag="VFF72_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL/Epsl periplasmic domain-containing protein"
                     /protein_id="HZV93200.1"
                     /translation="SSPAGVALTWANVDGVATVRLDGGLARALVPSPAPSTTRWSASP
                     AAAVTAEQWLGGAVAVMADEQRLLQAARSLWNLRQFELARRTRGARAMRDWLRQAMSP
                     AWRPVRIGLAGLLVAQIVGLNLWAWHERGVVDSRRAAIQSLVKSTFPRVSEADLQRDA
                     GAVAAREAQALRTLAGKPGDSDLEPMLQAAAAAWPAERPPVENLRFEPGKLTLGASGW
                     SDTQIEQFRAGLRQSGFQVDASEGRLVVSRPRLRGSA"
     gene            765..1292
                     /gene="gspM"
                     /locus_tag="VFF72_08285"
     CDS             765..1292
                     /gene="gspM"
                     /locus_tag="VFF72_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HZV93201.1"
                     /translation="MNMSNAAGSATLPPALNDARRQAGRFWQERAPRERQLIAAMVVA
                     VAVLLVWWITIQPALRILHQTPIEIDRLDQQLRQIQIAAAEMQTLRAASPVAAEQATA
                     ALRAATERLGSRARLTIQGERATLTFTGIPADALRAWLGEARSAARARPLEAQMVKAA
                     TGYSGSITVSVGSPA"
     gene            1289..2179
                     /gene="gspN"
                     /locus_tag="VFF72_08290"
     CDS             1289..2179
                     /gene="gspN"
                     /locus_tag="VFF72_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HZV93202.1"
                     /translation="MIRRKRVRARWSPTVAGSSWSESTLAEQAWEVQRGASVRWAVAG
                     ALVGVLVGIVLFAPAAWLASAVASASNQHILLAEAQGTIWSGSAVPVLAGGAGSRDAS
                     TLPGRLEWTLAPRLYGLELALRQPCCIDGTSLLQIRPGFGRIRATLVPPRGGAVGQWP
                     SAWLSGYGTPLNTLQLGGSFRVVTPGFTIEQVEGRWRLDGRVDLELLGVSSRLTTLDT
                     LGSYRVTLTGGSGANSATLLSLSTQDGPLQLTGSGTWGPGGIKFRGEARSASADEAAL
                     SNLLNIIGRREGARSIISIG"
     gene            2182..>4035
                     /gene="gspD"
                     /locus_tag="VFF72_08295"
     CDS             2182..>4035
                     /gene="gspD"
                     /locus_tag="VFF72_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HZV93203.1"
                     /translation="MIRFRARHPLAAARAGAAAAAAALLIASLPLAAAPAPRFRGAPV
                     TLNFVNADIEGVTRAMAAILRQQFIVDPRVKGTMTLYSEDPLSPNEAYLHFLAALRGL
                     GYTVVEAGGLYKVVPEADAKLQAGTVSIDGVSRHGDQVITQIFRLQYENANNLVPVLR
                     PLISPNNTINANPGNNTLVITDYADNLQRIAKIIAASDVPSTGDVEVVPLRYAIASDV
                     APLIQRLTDIPATPTAPGAANSLANNATSILVEPRSNSLIVRSASATKMATIRSLIAK
                     LDVQPEGSATSGNVWVVHLNNADAAKLATVLRAAFAAAVGGGGSGGGGGSSGGPTTSP
                     LAANAAGTAGTANATSAAAAPITATAGPSTGGFIQADPSTNSLIITAPEPLYRQVREM
                     IDKLDARRAQVYIESLIVEVSGTNAANVGFQWQGLLASGNANNGIVGGTNFGTTGNLI
                     SIDQAQLGGSGASAGGISLGEGLNVGIVHNFFGTYGLAAIANLLQSQSNTNIASTPNL
                     LTLDNEEAKIVVGSNVPFVTGQYTNTGTSGALSSPFQTIERKDVGITLRIRPQIGENG
                     TIRMSIYEESSSLSSQVAPGTSNAGPSTDKRSIESNVVVNDGQIVVLGGLIG"
BASE COUNT          620 a         1507 c         1358 g          550 t
ORIGIN      
        1 agctcgccgg ccggcgttgc gctcacctgg gccaacgtcg atggcgtggc cacggtccgg
       61 ctcgacggcg gcctcgcccg cgccctcgtg ccgtcgccgg cgccgtccac cacccgctgg
      121 tcggcgtcgc ccgccgccgc cgtgaccgcg gagcaatggc tcggcggcgc cgtcgccgtc
      181 atggccgacg agcagcggct gttgcaggcg gcgcgcagtc tctggaatct tcgccagttc
      241 gagctggcgc ggcgaacccg cggcgcacgc gccatgcgcg actggctgcg ccaggcgatg
      301 agcccggcct ggcgaccggt ccgcatcggc ctcgccgggc tcctggtcgc ccagatcgtc
      361 gggctcaacc tgtgggcctg gcacgagcgc ggcgtcgtcg acagccgtcg agcggcgatc
      421 cagtcgctcg tcaagtcgac ctttcctcgc gtcagcgaag ccgacctgca acgcgatgcc
      481 ggcgccgtcg cggcacgcga ggcgcaggcg ctgcgcaccc tggccggcaa gccaggcgat
      541 tcggacctcg agccgatgct gcaggcggcc gcggcggcct ggccggccga gcggccaccg
      601 gtcgagaacc tgcgcttcga gccgggcaag ctgacgctgg gtgcgagcgg ctggagcgat
      661 acgcagatcg agcaattccg cgccggcctg cgtcaatcgg gcttccaggt cgacgccagc
      721 gaaggacggc tcgtcgtctc gcggccacgg cttcgcggca gcgcatgaac atgagcaacg
      781 cagccggatc cgccaccctg ccgccggcgc tgaacgacgc ccgccggcaa gcgggccgct
      841 tctggcagga gcgggcgcca cgggagcgcc agctgatcgc ggcgatggtc gttgccgtcg
      901 ccgtgctgct ggtgtggtgg atcacgatcc agccggcgct gcgcatcctg caccagacgc
      961 cgatcgagat cgaccggctc gaccagcaat tgcgccagat ccagatcgcc gccgccgaga
     1021 tgcagacgct gcgcgccgcg tcgccggtgg ctgccgagca ggcgacggct gcacttcgcg
     1081 ccgccacgga gcggctgggc agccgcgcca ggctgacgat ccagggcgag cgcgccaccc
     1141 tcaccttcac cggcattccg gccgacgcgc tgcgcgcctg gctgggcgag gcgcgcagcg
     1201 ccgccagggc acgtccgctc gaagcccaga tggtcaaggc ggcgacgggc tacagcggct
     1261 cgatcacggt cagcgtcggg agtccggctt gatccgccga aagcgcgttc gcgcccgctg
     1321 gagtccgacc gtcgccggca gcagctggag cgagtcgacg ctcgccgaac aggcgtggga
     1381 ggtgcaacgg ggcgcctcgg tccgctgggc ggtggccggt gcgctcgtcg gcgtactggt
     1441 cggcatcgtc ctgttcgctc cggccgcatg gctcgcgtcg gcggtcgcat cggccagcaa
     1501 ccagcacatt ctcctggccg aagcgcaggg cacgatctgg tcgggcagcg cggttcccgt
     1561 gctggcgggc ggagccggca gccgcgacgc gagcacactt cccggccggc tcgagtggac
     1621 gctcgcgccg cgcctctacg gtctcgagct ggcgctgcgc cagccttgct gcatcgacgg
     1681 cacgtcgctg ctgcagatcc ggcccggctt cggccgcatc agggcgacgc tggtcccgcc
     1741 ccgcggcggc gccgtcgggc agtggccgag cgcatggctg agcggctacg gcacgccgct
     1801 caacaccctg cagctcggcg gcagcttccg cgtcgtcacg ccgggattca cgatcgagca
     1861 ggtcgagggc cgctggcgcc tcgacggccg cgtcgacctg gaactcctcg gcgtctcctc
     1921 gcgtctgacg acgctcgaca ccctcggcag ctaccgcgtg acactgaccg ggggatccgg
     1981 cgcgaacagc gccacgctgc tttcgctgtc gacccaggac ggcccgcttc agctcaccgg
     2041 cagcggcacg tggggcccgg gcggcatcaa gttccgtggc gaagcacggt cggcctcggc
     2101 cgacgaagcg gcgctctcca acctgctcaa catcatcggt cggcgcgaag gcgctcggtc
     2161 gatcatctcg atcggatgac catgattcga ttccgtgccc ggcacccgct ggccgccgcc
     2221 cgagccggcg ccgcggcagc tgccgcagcg ctcctcattg cgtcgctgcc gctcgccgcc
     2281 gcgccggcac cgcgctttcg cggcgccccc gtgacgctca acttcgtcaa cgccgacatc
     2341 gagggcgtga cccgggcgat ggccgccatc ctgcgccagc agttcatcgt cgacccgcgc
     2401 gtgaagggca cgatgacgct gtacagcgag gacccgctct cgcccaacga agcctacctg
     2461 cacttcctcg ccgccctgcg cggcctgggc tacaccgtgg tcgaggccgg gggcctctac
     2521 aaggtcgttc ccgaggccga cgccaagctg caggcgggca ccgtctccat cgacggcgtg
     2581 tcgcgacacg gcgaccaggt catcacccag atctttcgcc tgcagtacga gaacgccaac
     2641 aacctcgtgc cggtgctgcg gccgctgatc agcccgaaca acacgatcaa cgcgaacccg
     2701 ggcaacaaca cgctggtcat caccgactac gccgacaacc tgcagcgcat cgccaagatc
     2761 atcgctgcca gcgacgtgcc ttcgaccggc gacgtcgagg tcgtgccgct gcgctacgcg
     2821 atcgcctccg atgtcgcgcc gctgatccag cgcctgaccg acatcccggc cacgccgacc
     2881 gcgccgggcg cggccaactc gcttgccaac aacgcgacct cgatcctcgt cgagccgcgc
     2941 agcaactcgc tgatcgtgcg cagcgccagt gcgaccaaga tggcgacgat ccgctcgctg
     3001 atcgccaagc tcgacgtgca gcccgaaggc tcggcgacga gcggcaacgt ctgggtcgtg
     3061 cacctcaaca acgccgacgc ggccaagctg gcgaccgtcc ttcgcgcggc gttcgccgcc
     3121 gcggtcggag gcggcggtag cggcggcggc ggcggatcat cgggcggccc gacgacatcg
     3181 ccgctggccg ccaacgccgc cggcaccgcg ggaaccgcca atgcgacctc ggccgccgcc
     3241 gcgccgatca cggcgacggc gggcccttcg accggcggct tcatccaggc cgacccgtcg
     3301 accaactcgc tcatcatcac ggcgcccgag ccgctctacc gccaggtgcg cgagatgatc
     3361 gacaagctcg atgcgaggcg cgcgcaggtc tacatcgaaa gcctgatcgt cgaagtctcg
     3421 ggcacgaatg ccgccaatgt cggcttccag tggcagggcc tgctggccag cgggaacgcc
     3481 aacaacggca tcgtcggcgg aaccaacttc ggaacgaccg gcaacctgat ctcgatcgac
     3541 caggcacaac tcggtggcag cggcgccagc gccggcggca tctcgctggg cgaaggtctc
     3601 aacgtcggca tcgtccacaa tttcttcggc acctatggcc tcgccgcgat cgccaacctg
     3661 ctgcagagcc agtcgaacac caacatcgcc tcgacgccga acctgctgac gctcgacaac
     3721 gaggaagcga agatcgtcgt cggcagcaac gtgcctttcg tcaccggcca gtacaccaac
     3781 accggcacca gcggcgcgtt gagcagcccg ttccagacga tcgagcgcaa ggacgtcggc
     3841 atcacgctgc ggatccggcc gcagatcggc gagaacggca cgatccgcat gtcgatctac
     3901 gaggagtcgt cgagcctgtc gtcgcaggtc gcgcccggga cttcgaatgc cggcccctcg
     3961 accgacaagc gctcgatcga gtcgaacgtc gtcgtcaacg atggccagat cgtcgtcctc
     4021 ggcggattga tcggc
//
LOCUS       DASRWS010000439         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2755801, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000439 DASRWS010000000
VERSION     DASRWS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2755801"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFF72_08300"
     CDS             complement(<1..333)
                     /locus_tag="VFF72_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZV93204.1"
                     /translation="MAVACLTLPGMAWAQTGPQSLARIRLNAGIYNIDAELAQTAEER
                     EIGLMFRPTLGGSQGMLFVFERPGTQCFWMKNTLIPLDAAFIGDDGAIVNIAHMKPET
                     RDSHCSDRP"
     gene            434..1687
                     /gene="icd"
                     /locus_tag="VFF72_08305"
     CDS             434..1687
                     /gene="icd"
                     /locus_tag="VFF72_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZV93205.1"
                     /translation="MYQHIKVPKGGQKIKVNPDFSLVVPDEPVIPYIEGDGTGLDITP
                     VMIKVVDAAVAKSYGGRKKIHWMEVYAGEKSTRVYGPDVWMPAETLEAVRDYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPIQYFKGVPSPLKEPEKTNMVIFRENSEDI
                     YAGIEFEAESENATKLIKILQDQFGVRKIRFPATSGIGIKPVSREGTERLVRKAIQYA
                     IDNDKSSVTLVHKGNIMKFTEGAFRDWGYGLAQKEFGARPIDGGPLCKFKNPRNGKEI
                     VVKDVIADAFLQQILLRPAEYSVIATLNLNGDYVSDALAAQVGGIGIAPGANMSDSVA
                     MFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLIIAAMEKAILSKKVTY
                     DFARLMEGATQVSCSGFGQVMIEHM"
     gene            1861..2217
                     /gene="clpS"
                     /locus_tag="VFF72_08310"
     CDS             1861..2217
                     /gene="clpS"
                     /locus_tag="VFF72_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZV93206.1"
                     /translation="MPSDPPSTPTPPVARPRPDDGQGAVVVERQVQKTEPPRMYQVVL
                     LNDDYTPMEFVVMVLQEYFNRDLETATQIMLKIHHEGRGVCGVYSKDVAATKVELVLA
                     AARRGGHPLQCTMESA"
     gene            2214..4520
                     /gene="clpA"
                     /locus_tag="VFF72_08315"
     CDS             2214..4520
                     /gene="clpA"
                     /locus_tag="VFF72_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZV93207.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLMALLDNPSAAEVL
                     RACSANLDDLRKSLLGFIKENTPTVGGADEVDTQPTLGFQRVIQRAIMHVQSTGSGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFIAHGIKKSDPPEPSKPGDASGS
                     SADGDKDEGDGKGSPIDQFTQNLNQLARDGKIDPLIGREHEVERVVQILCRRRKNNPL
                     LVGEAGVGKTAIAEGLAWRITQNEVPEILADSVVYSLDMGALLAGTKYRGDFEQRLKG
                     VLKHLKDQPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGAMKCIGATTFTEY
                     RGIFEKDAALSRRFQKVDVVEPSIEQTVEILKGLKSRFEEHHSVKYATGALQAAAELS
                     AKYINDRHLPDKAIDVIDEAGAAQRILPKNKQKKTITRNEVEEIVAKIARIPPASVSN
                     DDRSKLKTLDRDLNSVVFGQEPAIDALAAAIKMARSGLGRPDKPIGSFLFSGPTGVGK
                     TEVAKQLAFVLGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAISKKPHA
                     VLLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGRKADFRSVIIVMTTNAGAETMNKSTI
                     GFTNSREAGDEMADIKRLFTPEFRNRLDATVSFKALDEEIILRVVDKFLLQLESQLAE
                     KKVEVTFTDVLRGHLAKKGFDPLMGARPMQRLIQDTIRAALADELLFGRLVDGGRLTI
                     DVDADGKPVLDIQPMKKSDKPKAEPATA"
     gene            complement(4525..5313)
                     /locus_tag="VFF72_08320"
     CDS             complement(4525..5313)
                     /locus_tag="VFF72_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HZV93208.1"
                     /translation="MPAWIQRWRQQRTLRRRPIPEPSWQLTLARFPFLTRRSEGDLLE
                     LRNMATLFLAQKEFAGMRGLEVTDEMAVAIAAQACLPALKLGLDWLDGFVGVVVHEDA
                     VVARREVEDEDGIVHAFDEELAGEAMQGGPVMLAWRDVDEAGESAAEGYNVVVHEFAH
                     VIDMRGGLTAGLDDVDPQSRRGIWLGSLADEFERFARQVDRNRPTFLDPYAAESLEEF
                     FAVAAEAFFVAPLDLARHQKRVYPLLRDAFRQDPAATWRPDATA"
     gene            complement(5324..>5480)
                     /locus_tag="VFF72_08325"
     CDS             complement(5324..>5480)
                     /locus_tag="VFF72_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZV93209.1"
                     /translation="APGSHSLALGRTRHVLSDWDLDATPSPRAEVLRWSAAGFERMQP
                     QAGPGRA"
BASE COUNT         1058 a         1785 c         1754 g          883 t
ORIGIN      
        1 tggcctgtcg gagcagtggc tgtcgcgcgt ctccggcttc atatgcgcga tgttgacgat
       61 cgcgccgtca tcgccgatga aggcggcatc gagcgggatc aaggtgttct tcatccagaa
      121 gcactgcgtc ccgggccgtt cgaagacgaa cagcatgccc tggctgcctc ccagcgtcgg
      181 ccggaacatc agcccgatct cgcgctcctc ggcggtctgc gccagttcgg cgtcgatgtt
      241 gtagatgccg gcgttgaggc ggatgcgcgc cagtgattgc ggtccggttt gcgcccaggc
      301 catgccgggc agcgtgagac aagccaccgc catgaggggc agcagcatcc gtagcgtgtg
      361 tttcatcgtt gagtgtccga cttagaatta caggttgcat tcatcacggc acgaccggcg
      421 gagcagcatt ctcatgtatc agcacatcaa ggtgcccaag ggcgggcaga agatcaaggt
      481 caaccccgac ttctcgcttg tcgttccgga cgaaccggtc attccttaca tcgaaggcga
      541 cggcacgggg ctcgacatca cgccggtgat gatcaaggtc gtcgatgcgg ccgtggcgaa
      601 gtcgtacggc ggccggaaga agatccactg gatggaggtc tacgccgggg agaagtcgac
      661 ccgggtctat ggccccgacg tctggatgcc ggccgagacg ctcgaggcgg tcagggacta
      721 tgtggtctcg atcaagggcc cgctgacgac ccccgtgggc ggcggcatcc gctcgctcaa
      781 tgtcgccctg cgccaggaac tcgatctcta cgtctgcctg cggccgatcc agtatttcaa
      841 gggcgtgcct tcgccgctca aggagccgga aaagaccaac atggtcatct ttcgcgagaa
      901 ctcggaagac atctacgccg gcatcgagtt cgaggccgag agcgagaacg cgacaaagct
      961 catcaagatc ctgcaggatc agttcggcgt caggaagatc cgctttccgg ccacttccgg
     1021 catcggcatc aagccggtgt cgcgggaagg aaccgagcga ctcgtcagga aggccatcca
     1081 gtacgcgatc gacaacgaca agtcttccgt cacgctggtg cacaagggca acatcatgaa
     1141 attcacggaa ggcgccttcc gtgactgggg ctacggcctg gcgcaaaagg aattcggcgc
     1201 ccgccccatc gacggcggcc cgttgtgcaa gttcaagaac cccaggaacg gcaaggagat
     1261 cgtcgtaaag gacgtgatcg ccgatgcgtt cctgcagcag atcctgctcc ggcccgcgga
     1321 gtactcggtg atcgccactc tcaacctcaa cggcgactac gtgtccgatg cgctcgcggc
     1381 gcaggtcggc ggcatcggca tcgcccctgg cgccaacatg agcgactcgg tcgcgatgtt
     1441 cgaagccact cacggcaccg cgcccaagta cgccggcaag gactatgtca acccgggctc
     1501 ggaaatcctc tcggccgaga tgatgctgcg ccacatgggc tggaccgagg cagccgatct
     1561 catcatcgcc gcgatggaaa aggcgatcct gtccaagaag gtgacctacg acttcgcccg
     1621 gctgatggaa ggcgccacgc aggtgtcatg ctcggggttc ggccaggtca tgatcgagca
     1681 catgtagggc gatccgatcc gatgcaagcc cgccgaatgg cgggcttttt tcttgccttc
     1741 gtgagacaac atgcgcggtt cgggccggcg cggaaacgaa gtcacgcgtt gccaggcgtc
     1801 cgtgggtgcc aatatgcgct caaggggcgc gttttggcgc cgactcgata gaatgaattc
     1861 atgccctccg acccaccgtc gacaccgact cctcccgtcg cccggccccg gcccgacgac
     1921 ggccagggcg cggtggtggt cgagcgccag gtccagaaga ccgagccgcc gcggatgtac
     1981 caggtggtcc ttctcaacga cgactacacc ccgatggaat tcgtcgtgat ggttttgcag
     2041 gaatatttca atcgcgatct cgagacggcg acacagatca tgctcaagat ccatcatgaa
     2101 ggccgcggcg tgtgcggcgt ctattcgaag gacgtcgccg ccaccaaggt cgagctggtt
     2161 ctcgccgcgg cgaggcgcgg cggtcacccg ctgcaatgca caatggagtc cgcatgattg
     2221 cgcaagagct tgaagtcagt ctgcacatgg cgttcgtgga agcacgccag cagcgtcacg
     2281 aattcatcac cgtcgagcac ctgctgatgg cgctcctcga caatccgtcg gcggccgaag
     2341 tgcttcgtgc ctgctccgcc aacctcgacg atctgcgaaa gagcctgctc ggcttcatca
     2401 aggaaaacac gcccaccgtc ggcggcgccg acgaggtcga cacgcagccc acgctcggct
     2461 ttcagcgggt gatccagcgc gcgatcatgc acgtgcagtc caccggcagc ggcaagaagg
     2521 aagtgaccgg cgccaatgtg ctggtcgcca tcttcggcga gaaggactcg cacgccgtct
     2581 actacctgca ccagcagggc gtgacccggc tcgacgtcgt caacttcatc gcccacggca
     2641 tcaagaagag cgatcctccc gagccgtcga agcccggcga tgcctcgggt tcgagtgcgg
     2701 acggcgacaa ggacgaaggc gacggcaagg gctcgccgat cgaccagttc acccagaacc
     2761 tcaaccagct cgctcgcgac ggcaagatcg atccgctgat cggccgcgag cacgaggtcg
     2821 agcgggtggt gcagatcctt tgccgccgcc gcaagaacaa tccgctgctc gtcggcgaag
     2881 ccggcgtcgg caagacggcg atcgccgagg gcctggcctg gcggatcacg caaaacgagg
     2941 tacccgagat cctcgccgac tcggtcgtct attcactcga catgggcgcg ctgctcgcgg
     3001 gcaccaagta ccgcggcgac ttcgagcaac gcctgaaggg cgttctcaag cacctgaagg
     3061 atcagcccaa cgcgatcctc ttcatcgacg agatccacac cctgatcggc gccggcgccg
     3121 cgtcgggcgg tacgctggac gcgagcaacc ttctcaagcc tgcgctcagc tccggagcga
     3181 tgaagtgcat cggtgcgacc acgttcaccg agtatcgcgg catcttcgag aaggacgcgg
     3241 ccctgtcgcg ccgtttccag aaggtcgacg tcgtcgaacc ttcgatcgag cagacggtgg
     3301 agatcctgaa gggtctgaag tcgcgcttcg aggagcacca cagcgtcaag tacgcgacgg
     3361 gtgccctgca ggcggccgcg gagctctcgg ccaagtacat caacgaccgg caccttcccg
     3421 acaaggcgat cgacgtgatc gacgaggcgg gcgcggcgca acgcatcctg cccaagaaca
     3481 agcagaagaa gacgatcacc cgcaacgagg tcgaggagat cgtcgccaag atcgcccgca
     3541 ttccgccggc gagcgtgtcg aatgacgacc gctcgaagct gaagacgctt gaccgtgacc
     3601 tcaacagcgt cgtcttcggc caggagcccg ccatcgacgc actcgccgcg gccatcaaga
     3661 tggcccgctc cgggctcggg cgccccgaca agccgatcgg ctcgttcctc ttcagcggcc
     3721 ccaccggcgt gggcaagacc gaggtcgcca agcagctggc gttcgttctc ggtatcgagc
     3781 tgatccgctt cgacatgagc gagtacatgg aacgccacgc ggtcagtcgc ctgatcggcg
     3841 cgccgcccgg ctacgtcggg ttcgaccagg gcggtctgct caccgaggcg atctccaaga
     3901 agccgcacgc ggtcttgctg ctcgacgaga tcgagaaggc gcaccccgat gtcttcaacg
     3961 tgctcctgca ggtgatggac cacggcacgc tcaccgacaa caacggccgc aaggccgact
     4021 ttcgcagcgt catcatcgtc atgacgacga atgccggcgc cgagacgatg aacaagtcga
     4081 ccatcggctt cacgaactcg cgcgaggcgg gcgacgagat ggccgacatc aagcgcctgt
     4141 tcacgccgga attcaggaat cgactcgatg cgacggtgag tttcaaggcg ctcgacgaag
     4201 agatcatcct gcgcgtcgtc gacaagttcc tgcttcagct cgagagccag ctcgccgaga
     4261 agaaggtcga agtcaccttc accgacgtac tgcgcggcca tctggcgaag aagggattcg
     4321 atcccctgat gggcgcccgg ccgatgcagc gcctcatcca ggacacgatc cgcgctgcgc
     4381 tggccgacga actgctgttc ggccggctgg tcgacggcgg ccgcctgacg atcgatgtcg
     4441 atgccgacgg caagccggtg ctcgacatcc agcccatgaa gaagagcgac aagcccaagg
     4501 ccgagccggc gaccgcctga tccatcaggc ggtcgcgtcc ggtcgccacg tggcggccgg
     4561 atcctgacgg aaggcgtctc gcagaagtgg gtagacgcgc ttctggtgtc tggccaggtc
     4621 cagaggcgcg acgaagaacg cttcggcggc gacggcgaag aactcttcga gcgattcggc
     4681 tgcgtagggg tcgaggaagg tcggtcggtt tcgatcgacc tgcctggcga agcgctcgaa
     4741 ctcgtcggcc agcgaaccca gccagatgcc gcgccggctc tgcggatcga cgtcatcgag
     4801 tccggccgtc aggccaccgc gcatgtcgat cacatgggcg aattcgtgga cgacgacgtt
     4861 gtagccctcg gccgccgact cgccggcctc gtcgacatcg cgccaggcga gcatcaccgg
     4921 gccgccctgc atggcctcgc cggcgagctc ctcgtcgaag gcgtggacga tgccgtcctc
     4981 gtcctcgacc tcgcgacgcg ccacgacggc gtcttcatga acgacgacac cgacgaagcc
     5041 gtcgagccag tcgagtccga gcttgagggc cggcaggcag gcttgtgcgg cgatcgcgac
     5101 agccatctcg tcggtgactt cgagcccgcg catcccggcg aactcctttt gcgccagaaa
     5161 gagggtcgcc atgttgcgga gctcgagcag atcgccctcg ctgcgcctgg tgaggaatgg
     5221 aaatcgggcc agcgtcagct gccacgaagg ctcgggaatc ggacggcgcc gcagcgtgcg
     5281 ctgctgtcgc cagcgctgga tccacgcagg cacggcgatc cgctcaggcg cgtcccgggc
     5341 cggcctgtgg ctgcatccgc tcgaatccgg ccgcgctcca gcgcagcact tccgcgcgag
     5401 gcgaaggcgt cgcgtcgaga tcccagtcgc tcaggacatg ccgtgtgcgg cccaaggcca
     5461 gggagtggct ccccggtgcg
//
LOCUS       DASRWS010000440         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3620788, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000440 DASRWS010000000
VERSION     DASRWS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3620788"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="virB11"
                     /locus_tag="VFF72_08330"
     CDS             complement(<1..964)
                     /gene="virB11"
                     /locus_tag="VFF72_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HZV93210.1"
                     /translation="MPHSQTLTHLLRPFQPYFDDASIEEIIVNRPGEVWIWRKGAFEC
                     HEIALDADDIIDIGIVAGAQRRQDVGPDKPLLATDLQGMGRLQVVLPPCVADGKPSLV
                     IRRGNAFSPSLDDLAIAGLFKSTKRRRGAASLEDDRLLRFYQEEDWPRFLREAVLAKK
                     TIVTCGETASGKTTVAKGLINAIPLNERLITIEDTPEWTDLPHRNQVNLFYSKDSHDV
                     TPRHLVAAALRMRIGRLLVQELRDGDAAFAFLRAVKSGHRGGVTTVHSDNPEAAFMAI
                     AVQMKETEAGASLDTEDVLAMLRQNIDVIAHCIRTDVDGITEFKV"
     gene            complement(964..2097)
                     /locus_tag="VFF72_08335"
     CDS             complement(964..2097)
                     /locus_tag="VFF72_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HZV93211.1"
                     /translation="MSDKPPPANPPASPVESATPVSGGKPPLSPVKIGLIIVACAAVL
                     MEGPRAFNWIRDATHSEPKQVAPAAAGPGQKYMPPEIPGTTAPKVTNAAVTVPPMPIE
                     SQGAQGQRPMDPRIAARLSDIGGGGGSATPPAPPGEHPPAGPLLPGVPVEQSALSKSL
                     TPTTLVASHSTEIPNPRFTIEQGRVLQCTQQTKINTSFPGGVTAILPEGVRGETGDVT
                     LLDPGSRVFGTIEHGMVNGLDRAFILWQNVTTPVMYDPDGTPRQIRITVNSPAADQLG
                     QGGLDGDVNHHYLRKFGAAIAFSLLQGGIQAGTAALAGGGGGGNGSLNSNYLNFQSGD
                     QVASQALSATVNIPDVLTRDQGLPCSIFIMRDLVIDNYQLKVR"
     gene            complement(2094..>2441)
                     /locus_tag="VFF72_08340"
     CDS             complement(2094..>2441)
                     /locus_tag="VFF72_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HZV93212.1"
                     /translation="PVVINATYTAQGTDEARTALAPNATWDDGRSTYFRFVGNRRLPV
                     PYVLNPDGEEAVVDYTVQDRTLIIHKTAARFRLSDGDLILCISNGNYTPGGVNTGTGT
                     TSPDVVREMKASK"
BASE COUNT          369 a          773 c          832 g          467 t
ORIGIN      
        1 tcaccttgaa ctcggtgatc ccgtcgacgt ccgtgcggat gcaatgcgcg atcacgtcga
       61 tattctgccg cagcatcgcg agaacgtcct cggtgtctag cgatgcgccc gcctccgtct
      121 ccttcatctg caccgcgatc gccatgaagg cggcctcggg attgtcggaa tgcacggtgg
      181 tcacgccgcc cctgtgccct gacttgacgg cgcgcaggaa ggcaaacgcg gcgtctccgt
      241 cgcgcaattc ttgcacgagc aagcggccga tccgcatacg cagcgcggcg gcgacaaggt
      301 ggcgcggcgt gacgtcgtgg gagtcctttg agtagaaaag attgacctgg ttgcgatgcg
      361 gcaggtcggt ccattccggt gtgtcctcga tggtgataag ccgttcgttg agcgggatcg
      421 cgttgatcag ccctttggcg acggtcgttt tgccgctcgc ggtctcgccg caggtcacga
      481 tggttttctt tgccagcact gcctcccgca agaagcgggg ccagtcctct tcctggtaga
      541 accgcagcag cctgtcgtcc tccaatgacg ccgcgccgcg ccgccgcttg gtgctcttga
      601 acaggccggc gatggcgaga tcgtcgagcg acggtgagaa agcattgccg cgccggatga
      661 caagcgacgg tttgccgtcg gcgacgcagg gcggcagcac gacctggagg cgtcccatgc
      721 cctgcaagtc ggtcgcgagc agcggcttgt ccggtccgac gtcctgccgg cgctgggcgc
      781 ccgcgacgat gccgatgtcg ataatgtcgt cggcatcgag cgcgatctcg tggcactcga
      841 aggcgccctt tcgccaaatc cagacctcgc cgggccggtt gacgatgatc tcctcgatac
      901 tcgcatcatc gaaatacggc tggaacggcc gtaggaggtg ggtcagcgtt tggctgtggg
      961 gcattacctg actttcaact ggtagttgtc gatgacgaga tcgcgcatga tgaagattga
     1021 gcacggcaag ccctgatcgc gcgtcagcac gtcggggatg ttcaccgtcg ccgagagcgc
     1081 ttgcgatgcg acctgatcgc cgctctggaa attcaggtag ttcgagttga ggctgccgtt
     1141 gccgccgcca ccgcctccgg cgagcgccgc cgtgccggcc tgaatgccgc cctgcaacag
     1201 gctgaaggcg attgcggcgc cgaacttgcg caggtagtgg tggttgacgt cgccatcgag
     1261 gccgccctgg cccaactggt cggcggcagg actgttgacg gtgatcctga tctgccgtgg
     1321 cgtgccgtcg ggatcataca tgacgggcgt ggtcacgttc tgccaaagga tgaaggcgcg
     1381 atcgagaccg ttgaccatgc cgtgctcgat agtgccgaac acgcggctgc cgggatcgag
     1441 cagcgtcacg tcccccgtct cgccgcgcac gccttccggc aggatcgcgg tcacgccgcc
     1501 gggaaagctc gtgttgatct tggtctgttg cgtgcattgc agcacgcgcc cttgctcgat
     1561 ggtaaagcgg gggttgggga tctccgtcga atggctcgcg accagcgtcg tcggcgtcag
     1621 cgacttcgat agcgctgatt gctccaccgg cactcccggc agtagcggcc cggccggcgg
     1681 atgctcgcct gggggcgctg gcggcgttgc gctgccgccg ccgccgccaa tgtccgacaa
     1741 gcgggcggca atacgcgggt ccatcggccg ctgcccctgc gccccttgcg attcgatcgg
     1801 catcggcggc acggtgacgg cggcattcgt caccttcggc gccgtggttc ccgggatttc
     1861 aggcggcatg tatttttggc ccggaccggc cgcagcaggc gcgacctgct tgggctcgga
     1921 gtgtgttgcg tccctgatcc agttgaaggc gcgcggccct tccatcagca ccgccgcgca
     1981 agccacgata atcaggccga tcttgaccgg cgacagcggc ggcttgccgc ccgaaaccgg
     2041 cgtggcgctt tcgacgggcg aggccggcgg gttggccggc ggcggcttgt cgctcatttg
     2101 ctcgccttca tttcccgaac cacgtcaggg ctcgttgtcc cggtgccggt gttgaccccg
     2161 ccgggcgtgt agttgccgtt ggagatgcag aggatcaggt cgccgtcgct caagcggaac
     2221 cgcgccgccg tcttgtggat gatcagcgtg cgatcctgga cggtgtaatc gaccaccgct
     2281 tcctcgccgt cgggattgag cacgtagggg accggcaggc gccgattgcc gacaaagcgg
     2341 aagtaggtcg agcggccgtc atcccacgtc gcattgggcg ccagcgccgt gcgcgcttcg
     2401 tcggtgccct gcgcggtgta ggtggcattg atgacgaccg g
//
LOCUS       DASRWS010000441         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1040751, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000441 DASRWS010000000
VERSION     DASRWS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040751"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(740..1099)
                     /locus_tag="VFF72_08345"
     CDS             complement(740..1099)
                     /locus_tag="VFF72_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93213.1"
                     /translation="MPDSSNTTLSFRVRHGLCVHRQRIVKIQTGRSAVSTAEVVSFGA
                     PFGRDTVDLEPGEVKRYAHALEPTCEAGARALQALHRQAPPVATEDETPREARIVAAV
                     VAKLIAAGALKFAKPAS"
     gene            complement(1138..2382)
                     /locus_tag="VFF72_08350"
     CDS             complement(1138..2382)
                     /locus_tag="VFF72_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HZV93214.1"
                     /translation="MEISIANHAEVRARLAHIASVASAASESLGARKASPADLVKDLS
                     KKEVEGFSFARAIRACLEGDWQKAGLEREISNALCDLRKEPAAPRSFLVPSALPFQVR
                     GAPRYSERAIYQVGTAGAGGNLVETELLADDFVAVLRNSTVVGQLGARFLPGLVGDVN
                     IPRQDTATQTYWVGESVALTESEATFDQVQLKPHTVGALSKISRQMILQSTPAIEQLV
                     RNDLVETSALAVDAAALYGTGTSSQPTGIANTPDVGAVVMGTNGGDYTFDALVSLYAA
                     PLVANAPQANLAYAINGKVRGYLATLKSSTGQYLWNPSQGIAGASPNSLLGYRYGVSN
                     QLPYNLTKGTSAGICSMSIFGNWQELLIGEWGVLEFMVNPYDSTGFANGDVVVRAMQT
                     IDVGLRHPESFAVLSDGLTPGF"
     gene            complement(2466..2858)
                     /locus_tag="VFF72_08355"
     CDS             complement(2466..2858)
                     /locus_tag="VFF72_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93215.1"
                     /translation="MKIAEAAAARGLHVDALRRLARRHRNAFVVQRGTGGRGKSTMVD
                     LSLLDAVLGIQGRDDTGSQRALRAEAALKRLPGIIRARFAEALRDGSLTGAPYFCKPS
                     AAEWIVQYLSTEAELAIQYEGTTAPPDA"
     gene            complement(2888..3457)
                     /locus_tag="VFF72_08360"
     CDS             complement(2888..3457)
                     /locus_tag="VFF72_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93216.1"
                     /translation="MAEVDVYALAKAMNLTPRRVYQLRHEGLPAVKPGRYDLGRCLWW
                     YIRYLQAAVERQNGTPNGGLSLTAERARLAREQANRLALQNEERRAATYPAELVHRTL
                     LEQREEFARLLDKHVNEDDALVEWLRTQTDPGRVRQELQVRIRAVRSDMADFIRKQTR
                     ATCEEPNNFALPGARGEPSRPSRKRSRKA"
     gene            complement(3887..4075)
                     /locus_tag="VFF72_08365"
     CDS             complement(3887..4075)
                     /locus_tag="VFF72_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93217.1"
                     /translation="MTAEPLDWQERADRHRPQAAADMTAEVRRLAGSGLKARDISTAL
                     RLDLAVVMQALQEEPAHG"
BASE COUNT          692 a         1347 c         1321 g          787 t
ORIGIN      
        1 cccagcgtcc ggtgaaatgg agtgcttgga ggcgcgattg cggtgctccc gacgccaggc
       61 cagatgaagc cgcgtgcgcc tcccatgtcg ccacgacaag agcggactaa aaggcgctca
      121 gcgtcgctat tcatcgtctc gcacttcacc tgccgttcgg aacagtcgcc tccgtactcc
      181 cgtgtccaac gccgcggggt cgaggaaggt ccggtcgtac tttgccgatg gccgttatgt
      241 ggcctggcag ccggcatcgt cgtcgaggag cggcgatgcc agcgatccat ctgggcttaa
      301 agctggcgag agcatccatt caggtggccg gcatccttgt tctcgtcgat gaaacgctga
      361 gccgcgctgg agtcggcgaa cgtcgctacc caaacatcgc cggcagaatt caccacctca
      421 gtgccctttg gtgtgtcttt cgtgaccagg tcagccgaat taactattac aagcgtcatg
      481 gcgaaattcc ttggctcgat gagagtccgc ctccgtgctc ccgtccatca cagccggggt
      541 cgaggaggcg ccgtaaacgc gcccacgtgg atcgcgcttg ccgatgacct gtctcgtcgg
      601 acttcacggc ccgcacccgc cgtaggttgt cgcctccgct gaggcttccg gcgctgccag
      661 gctggccccg ttcggctatg cgggcacggt ggccttatcc cgtcccccgt cgatagagaa
      721 agcgggggag aactcgttgc tacgatgcag gctttgcgaa cttgagtgcg ccggctgcga
      781 tcagctttgc cacaacggcc gctacgatcc gtgcctcccg cggcgtctcg tcctctgtgg
      841 cgaccggcgg cgcctggcga tgcagcgcct gcaacgcgcg cgcgccggct tcgcaagtgg
      901 gctccagggc atgggcgtaa cgcttcacct cgccgggctc gaggtcgacg gtatcgcggc
      961 cgaagggcgc gccgaaacta acgacctccg cggtgcttac cgcggagcgg ccggtctgga
     1021 ttttcacgat gcgctggcgg tggacgcaca gtccatggcg cacgcggaag ctcaatgtcg
     1081 tgttgcttga atcgggcatg attgtcctcg ctaactcagt tgctgccagt ccgccgctta
     1141 gaagcctggg gtgaggccgt ccgacagaac ggcgaaggat tccgggtgcc ggaggccgac
     1201 gtcgatcgtc tgcatagcgc gcaccaccac atcgccgttc gcgaagccgg tcgagtcgta
     1261 ggggttgacc atgaactcca acacgcccca ctcgccgatc agcagctcct gccaattgcc
     1321 gaagatgctc atcgagcaga tgccggcgct ggtgcccttg gtcaggttgt agggcaactg
     1381 gttgcttacc ccatagcgat agcccagcag gctgttcggc gaggcgcccg ctatgccctg
     1441 gctcggattc cagaggtact gaccggtgga gctcttcagg gtcgccaggt atcccctgac
     1501 tttcccgttg atcgcgtatg ccaaattcgc ctgcggcgcg ttcgcgacca ggggcgcagc
     1561 gtagaggctg accaaagcgt cgaaggtgta gtcgccgcca ttggttccca ttacgacagc
     1621 gcccacgtcc ggtgtattcg cgatgccagt cggctgcgag ctcgtgccgg tgccatacag
     1681 cgcagcggcg tcgaccgcca gggccgatgt ctccacgaga tcattccgga cgagctgttc
     1741 aatcgcgggc gtgctctgga ggatcatctg gcgcgatatc ttggacagcg cacccacagt
     1801 gtgcggcttg agctgaacct gatcgaaggt cgcttcgctt tccgtgagcg ctacggactc
     1861 gcctacccag taggtctggg tggcggtgtc ctggcgcgga atgttgacat caccgaccaa
     1921 gccggggagg aaacgtgcgc cgagctggcc cacaaccgtg gaattgcgca gcactgcgac
     1981 aaagtcgtcg gccagcagct cagtttccac cagattgccg ccggcaccag cggtgcccac
     2041 ttgatagatc gcgcgctccg aatagcgggg agcgccgcgc acctgaaaag gcagcgcgct
     2101 tggaaccagg aagctgcgcg gtgcagcggg ctccttccgc agatcgcaca gcgcattgga
     2161 gatctcacgc tcgagcccgg ctttctgcca gtcgccctcc aggcacgcgc gaatggcgcg
     2221 cgcgaacgag aagccctcga cttctttttt gctcagatcc ttcaccagat ccgcggggct
     2281 ggcctttcgg gctccgagag attcgctggc agcgctcgcg accgatgcaa tgtgcgccag
     2341 gcgcgcgcgg acttccgcgt gattggcaat gctgatttcc atttgtcgtc aactcatatc
     2401 gcgcaacgct gcgcactgat gaccgttata cgcaagcgac ctaaacttag tggtcagcgc
     2461 taggtttacg cgtcgggcgg cgccgtcgtg ccttcgtact ggatcgcgag ctcagcttcg
     2521 gtggataggt actgcacgat ccattccgca gcgctcggct tgcaaaaata gggcgcgcca
     2581 gttagagacc cgtcgcgaag ggcttcggcg aagcgcgctc ggatgatccc cggcaaccgt
     2641 ttcagcgccg cttcagcgcg cagggctcgc tggctcccag tatcgtcgcg gccttgtatg
     2701 cccagcacgg catcgagcag cgagaggtcg accattgtcg acttgccgcg acctccagtg
     2761 ccgcgctgga ccacgaaagc gtttcgatgg cgacgcgcga ggcgacgcaa cgcgtcgacg
     2821 tggaggccgc gggctgccgc cgcctccgcg atcttcacga ttgtcgcctg cgctggcatc
     2881 gcccctgtta cgccttccgg gagcgtttgc gagaggggcg agacggctcg ccgcgcgcgc
     2941 cgggtaacgc gaagttattg ggttcctcgc aagtggcgcg ggtctgcttc ctgatgaagt
     3001 cggccatgtc ggatcgcacg gcgcgaatac ggacttgcag ctcctgccgg acgcgcccgg
     3061 ggtcggtctg ggtccgcagc cattcgacaa gcgcatcgtc ctcattgacg tgtttgtcga
     3121 gcagacgggc gaactcctcg cgctgctcca gcagggtgcg atgcacgagc tcggcgggat
     3181 acgttgcggc tctgcgctct tcgttctgca gtgcgagtcg gttcgcctgc tcgcgggcta
     3241 ggcgcgcgcg ctcggcagtg aggctcagcc caccattagg cgtgccgttt tgccgttcga
     3301 cagcggcctg cagatatcgg atataccacc agaggcagcg gcccagatcg taccgaccag
     3361 gcttgacggc cggcaggccc tcgtgccgca gttggtaaac cctgcgcggc gtgagattca
     3421 tcgccttcgc gagcgcgtaa acatcgactt cagccattgc gtaactccct gtcgtcagtg
     3481 aagtgaaacc ctaggggcgg acggaacagc ccagatcctt gcggctcaag gcgatcgcac
     3541 tcctcctcgg agaaggaccc gccgatccga cggcagtacg ggtagtcagt ccggcttcag
     3601 gccggcccgc agatgtcgac ggccgccatg ccacgctgtt ggcgctcact ctgcctcctc
     3661 gctgtgtgcg ctctgggcta tggcctgtgc cgctagtcgc ctctccagcg ctgcctcagc
     3721 ccgtctggca gtgatcggct tggcgggcat gcatcgccag cctctgctcg tcaggcgcgc
     3781 catatggccg cggtgccaac actggacgag cggcggcagt ggccgagtag cgcgccacgt
     3841 ggcaaagcaa ttcgggcaaa tctcatccgg gcggggcggc actgaatcag ccatgggccg
     3901 gctcctcctg aagagcctgc atcaccacag ccaggtcgag ccgtagtgcc gtactgatgt
     3961 cacgcgcttt caggccgcta ccggccagtc ggcgcacctc tgcagtcata tcggccgcgg
     4021 cttgcgggcg atgacggtcc gcgcgctctt gccagtccaa cggttctgca gtcatgtgca
     4081 tcgatcagcc ctcctgtgac cgaggccact cttcgaggaa tgccagctcc ggtcctaaaa
     4141 acgtgct
//
LOCUS       DASRWS010000442         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2203156, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000442 DASRWS010000000
VERSION     DASRWS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203156"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..978
                     /locus_tag="VFF72_08370"
     CDS             94..978
                     /locus_tag="VFF72_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93218.1"
                     /translation="MAVRDGAALDVGVDTSPVALLGRRRRVGGLFIASLIWIALVALG
                     AIFADLLPIPSPTDMDMLGKRALPSAEHWLGNDVLGRDVFSRLIYGGRISLTVGLLAP
                     VLGVTIGGALGLLAGYFRGRLETFITGGVDVLLAFPPLVFALAVTAYLGQSIANITLL
                     IGVLGIPAFTRVARAVTLSLSEREFVTAARALGATHSRIILRELLPNVALPLVAFFLL
                     GVAVTIVVEGALSFLGLGVPPPAPSWGSMIGEGRESLDIAPWLAFLPALFMFLTVLAF
                     NVVGDTLRALTDPRPGAL"
     gene            992..1966
                     /locus_tag="VFF72_08375"
     CDS             992..1966
                     /locus_tag="VFF72_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93219.1"
                     /translation="MSAAPILSIQGVSVTLPTPRGLLRAVDGVDLTLPPGRTLGVVGE
                     SGCGKTMLSRAILQLLPKRAALKGRVMFDGRDLAGLPAEELRRLRGPELAVVFQDPMT
                     SLNPVLTIGTQIIETLQAHLGLDAANATRRAIELLAEVGIPAPAQRLRQYPHQLSGGM
                     RQRVAIAIALSCEPKLLIADEPTTALDVTVQAQILDLLAREQQRRHMAMILITHDLGV
                     VAGRTDEVAVMYAGRVVERAPTPRLFRHMRMPYTEALLAAIPKLDSAPHTPLPAIGGR
                     PPDPTRPLPGCSFAPRCKYADSVCGHTKPTLEDPEGVGALFACWHPLT"
BASE COUNT          239 a          767 c          663 g          346 t
ORIGIN      
        1 tgcagggcgc ggtgctgttc gtcgccgtcg gcttcgtgat ggtgaacttc atcgtcgaca
       61 tgctttacgc cgtcctcgac cccaggatcc gccatggccg tgcgtgacgg ggccgcgctc
      121 gacgtcggcg tcgacacctc gccggtcgcc ctgctcggcc ggcggcgccg cgtcggcggc
      181 ctgttcatcg cctcgctgat ctggatcgcc ctggtggcgc tcggcgccat cttcgccgac
      241 ctgctgccaa tcccgagccc gactgacatg gacatgctgg gcaagcgtgc gctgcccagc
      301 gccgagcatt ggctgggcaa cgacgtgctg ggtcgcgacg tgttctcgcg cctgatctat
      361 ggcggccgca tctcgttgac ggtcggcctg ctcgccccgg tgctcggcgt cacgatcggc
      421 ggcgcgcttg gtctgctggc cggctacttc cgcggccggc tcgagacctt catcaccggc
      481 ggcgtggacg tgctgctcgc cttcccgccg ctggtgttcg cgctcgccgt caccgcctat
      541 ctcggccagt cgatcgccaa catcaccctg ttgatcggcg tgctcggcat cccggccttc
      601 acccgcgtgg cgcgcgcggt gacgctgtcg ctcagcgagc gcgagttcgt caccgcggcg
      661 cgcgccctcg gcgccaccca cagccgcatc atcctgcgcg agctgctgcc caacgtcgcg
      721 ctgccgctgg tcgccttctt cctgctcggc gtggcggtga ccatcgtcgt cgagggtgcg
      781 ctctccttcc ttggcctggg cgtgccgccg ccggccccgt cgtggggcag catgatcggc
      841 gagggccgcg agagcctcga catcgcgccg tggctcgcct tcctgccggc actgttcatg
      901 ttcctgacgg tgctcgcctt caacgtcgtc ggcgacacgc tgcgcgcgct gaccgatccg
      961 cgccccgggg cgttatagtg ggcgccctct gatgagcgcc gcgccgattc tttcgatcca
     1021 gggcgtttcg gtcaccctgc cgacgccgcg cggtttgctg cgcgcggtcg acggcgtcga
     1081 tctcacgctg ccgcccggcc gcaccctggg ggtggtcggt gaatcgggct gcggcaagac
     1141 catgctgtcg cgcgccatcc tgcagctcct gcccaagcgc gcggcgctga agggccgcgt
     1201 gatgttcgac ggccgcgacc tcgcgggcct gccggccgag gagttgcgtc gcctgcgcgg
     1261 gcccgagctg gcggtggtct tccaggaccc gatgacctcg ctcaatcccg tgctgaccat
     1321 cggcacgcag atcatcgaga ccctgcaagc ccatctcggc ctcgacgccg ccaacgcgac
     1381 gcggcgcgcc atcgagctgc tggccgaggt cggcatcccc gcgccggcgc aacgcctgcg
     1441 ccagtacccg catcagctct ccggcggcat gcgccagcgc gtggcgatcg cgatcgcact
     1501 atcctgcgag cccaagcttc tcatcgccga cgaaccgacc acggcgctcg acgtcaccgt
     1561 gcaggcgcag atcctcgacc tgctggcgcg cgagcagcag cgccgtcaca tggcgatgat
     1621 cctgatcacg cacgatctcg gcgtcgtcgc cggccgcacc gacgaggtcg cggtgatgta
     1681 tgccggccgc gtcgtcgagc gcgcgccgac accgcgcctc ttcaggcaca tgcgcatgcc
     1741 gtacaccgag gccttgctgg ccgcgatccc caagctcgac agcgcgccgc acacgccatt
     1801 gccggcgatc ggcggccgcc cgcccgatcc gactcggccc ttgcccggat gctcgttcgc
     1861 cccgcgctgc aagtacgccg actccgtgtg cgggcacacc aagccgacgc tcgaggatcc
     1921 cgaaggggtg ggcgcgctgt tcgcctgctg gcatccgctg acatgagccc gctgctcgcg
     1981 atcgacgacc tcaccgtcga gtacgacgtc ggcgg
//
LOCUS       DASRWS010000443         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3621337, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000443 DASRWS010000000
VERSION     DASRWS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3621337"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2276
                     /locus_tag="VFF72_08380"
     CDS             <1..2276
                     /locus_tag="VFF72_08380"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HZV93220.1"
                     /translation="VRVPESSCMPGFYRLSVLDRLDLVHERGLLSREDRHALVSGNHT
                     LDIQRADKMIENVIGVMGLPVGLGLNFLINGKDYVVPLAVEEPSIVAALSSAAKTIRG
                     AGGFTTESTDPILIGQVQVVDVASSAKARAALLQKKDEILNLANSLHPNMVARGGGAE
                     DVEVMVHAAPDGGRDMVVLHLLVNTCDAMGANLVNTMCEGVAPLVESITGGKVFLRIL
                     SNLSDRAVVRARVTIPTRLLSAKGRAGADVRDGIVLANDLALVDPYRAATHNKGIMNG
                     VDAVALATGNDWRALEAAAHAYAARGARGYSALTSWTRNDAGDLVGSIEMPMKVGTVG
                     GPLQSNRTVALNHRLLGIGSARELAEVMGAVGLAQNFSALRALVTDGIQQGHMTLHAR
                     SVAMAAGATADVFNAVVERLLDSGEIKIWKARELIAEIANGARGPAPAKATRAARAVA
                     REDWSAGHGKVILLGEHAVVYGRHAIAAPLPLAIHARAEDGRGETTLVIPRWGIEQRL
                     QRGVDRPGSLLQSLARVLERLGLADRSLKVHVQPSVPRAMGLGSSAALAVAIIRAVDR
                     HCRLGLDDDAVCELAYECEKIAHGTPSGVDNTVAVRGRPLLFRRGSPPLERTLTIHRP
                     LPLVIGMSGTESLTAKMVARVRRARERNPELYERIFDAMDALTLGAIEAIEADDLGQL
                     GELMNLCQGQLNALQVSSAEVENMLQIARDNGAAGAKLTGGGGGGSMIALCPDGCARV
                     TQALQVAGYRTMEAHLG"
     gene            2269..2856
                     /gene="idi"
                     /locus_tag="VFF72_08385"
     CDS             2269..2856
                     /gene="idi"
                     /locus_tag="VFF72_08385"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZV93221.1"
                     /translation="MAELLRDDAVVSFENEELVLVDAEDREVGHLSKSACHDGSGLRH
                     RAFSIFVFDKAGDVLLQKRSRGKRLWGGYWANSCCSHPRRGETMALATRRRLREELGL
                     ECALEFVFKFEYQAGFRGIGAEHELCWVYVGTATAPVRANRNEIEAWRFVSPAALDSE
                     VADHPERFTPWLKLEWRRLREEFGARLPLPASESR"
     gene            2853..>3307
                     /gene="hpnH"
                     /locus_tag="VFF72_08390"
     CDS             2853..>3307
                     /gene="hpnH"
                     /locus_tag="VFF72_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZV93222.1"
                     /translation="MSIPLMQKLRVGGYVLGKKIRRVKRYPLALMLEPLFQCNLACPG
                     CGKIDYESDILERRMSVEQALNAVDECGAPVVSIPGGEPLIHKEIGQIVDGIVARRKF
                     VYVCTNAILLKQKLHLFKPSPYLTFSVHLDGDRERHDRSVDRAGVFDKAV"
BASE COUNT          525 a         1144 c         1134 g          504 t
ORIGIN      
        1 gggtccgcgt tcccgaaagc tcgtgcatgc cgggctttta ccggctctcg gtgctcgacc
       61 ggctcgacct cgtccatgag cgcgggctct tgtcgcgcga ggatcgccat gccctcgtca
      121 gcggcaatca cacgctcgac atccagcgcg ccgacaagat gatcgagaac gtcatcggcg
      181 tgatgggact gccggtcggc ctcggcctga atttcctcat caacggcaag gactatgtcg
      241 tgccactcgc cgtcgaggag ccgtcgatcg tcgccgcctt gagctcggcc gccaagacca
      301 ttcgcggcgc cggcggcttc acgaccgaaa gcacggaccc gatcctgatc ggccaggtcc
      361 aggtcgtcga tgtcgccagc tccgccaagg cacgagcggc gctgctgcag aagaaggacg
      421 agatcctcaa tctcgccaac agcctgcacc cgaacatggt cgcgcgcggc ggcggcgccg
      481 aggacgtcga ggtgatggtg cacgccgcgc ccgacggcgg ccgcgacatg gtcgtcctgc
      541 atctgctcgt caacacctgc gatgccatgg gcgccaatct ggtgaacacg atgtgcgagg
      601 gcgtcgcccc cctggtcgaa tcgatcaccg gcggcaaggt gttcctgcgc atcctctcga
      661 acctgagcga ccgggccgtc gtccgcgcac gcgtcacgat ccccacgcga ttgctctcgg
      721 ccaagggccg ggccggcgcc gacgtgcgcg acggcatcgt cctggcgaac gacctggcgc
      781 tcgtcgatcc ctatcgcgcc gcgacgcaca acaagggaat catgaacggc gtcgatgccg
      841 tcgcattggc cacgggcaac gactggcgcg ccctggaggc ggcggcgcac gcgtacgccg
      901 cgcgcggcgc gcggggctac agcgccctga cgagctggac caggaacgat gccggcgacc
      961 tggtcggcag catcgagatg ccgatgaagg tcggcaccgt cggcggtccc ctgcaatcga
     1021 atcgcacggt ggcgctgaac catcgcctgc tcggcatcgg ctcggcgcgc gagctcgccg
     1081 aggtgatggg cgcggtcggc ctggcacaga acttctcggc gctgcgcgcc ctcgtgaccg
     1141 acggcatcca gcagggccac atgactctgc acgcacgcag cgtcgcgatg gccgccgggg
     1201 cgacggccga tgtcttcaat gccgtcgtcg aacgtctcct cgacagcggc gagatcaaga
     1261 tctggaaggc acgcgagctc atcgccgaga tcgcgaacgg tgcgcggggg ccggcgccgg
     1321 ccaaggcgac gcgcgccgcc cgcgccgtcg ccagggagga ctggtcggcc ggccacggca
     1381 aggtgatcct gctgggcgag catgccgtcg tctacggccg ccatgccatc gccgcgccgt
     1441 tgcctctggc gatccacgct cgcgccgagg acggccgcgg cgaaacgacg ctggtcattc
     1501 cgcgctgggg catcgagcag cgattgcagc gcggcgtcga ccgccccggc tcgctcctgc
     1561 agtccctcgc gcgcgtcctc gagcggctcg gcctcgccga tcgcagcctg aaggtgcacg
     1621 tgcagccgag cgtgccgcgg gcgatgggcc tgggcagctc ggcggcgctc gccgtcgcga
     1681 tcatccgcgc cgtcgaccgc cattgccgcc tcggcctcga cgacgacgcg gtctgcgagc
     1741 tggcctacga gtgcgagaag attgcccacg gcacgccgtc cggtgtcgac aacacggtgg
     1801 cggtgcgcgg ccggccgctg ctgtttcgtc gcggcagtcc gccgctcgag cgcacgttga
     1861 ccatccatcg gccgctgccg ctggtgatcg gcatgagcgg caccgagagc ctgacggcga
     1921 agatggtggc gcgcgtgcgc cgcgcccgcg agcgcaaccc cgagctctac gagcggatct
     1981 tcgacgcgat ggacgcgctg accctcggcg ccatcgaggc gatcgaggca gacgacctcg
     2041 gccagctcgg cgagctgatg aatctctgcc agggccagct caacgcgttg caggtctcga
     2101 gcgccgaggt cgagaacatg ctgcagatcg cgcgcgacaa cggcgccgca ggtgccaagc
     2161 tgaccggcgg cggaggtggc ggctcgatga tcgcgctgtg ccccgatggc tgcgcccgcg
     2221 tcacgcaggc gctgcaggtt gcgggatacc ggacgatgga ggcacatctt ggctgaactg
     2281 ctgcgcgacg atgcggtcgt ctcgttcgag aacgaggagc tcgtcctcgt cgacgccgag
     2341 gaccgcgaag tgggccatct gagcaagtcc gcctgccacg atggcagcgg cttgcgtcac
     2401 cgggcctttt ccatcttcgt cttcgacaag gcgggcgacg tcctgctgca gaagcgaagc
     2461 cgcggcaagc gcctctgggg cggctactgg gccaacagct gctgcagcca tccgcgtcgc
     2521 ggcgagacga tggcgctggc cacccggcgc cgcctgcgcg aagagctcgg cctcgaatgc
     2581 gcactggagt tcgtcttcaa gttcgagtac caggccgggt ttcgcggcat cggcgccgag
     2641 cacgagctct gctgggtcta tgtcggcacg gccacggcac cggtgcgtgc caaccggaac
     2701 gagatcgagg cctggcgctt cgtgtcgccg gccgctctcg acagcgaggt ggccgaccat
     2761 cccgagcgtt tcacgccgtg gctgaagctc gagtggcggc gactgcgcga agagttcggc
     2821 gcccgcctgc cgctgccggc atcggagtcc agatgagcat tcccctcatg caaaagttgc
     2881 gcgtcggcgg ctacgtgctc ggcaagaaga tccgtcgtgt caagcgctat ccgctggcgc
     2941 tgatgctcga gccgctcttc cagtgcaacc tggcgtgtcc cggttgcggc aagatcgact
     3001 acgaaagcga catcctcgag cggcggatga gcgtcgagca ggccctgaac gcggtcgacg
     3061 agtgcggcgc gccggtggtc tcgattccag ggggcgagcc gctgatccac aaggagatcg
     3121 gccagatcgt cgacggcatc gtcgcgcgac gcaagttcgt ctacgtgtgc accaacgcca
     3181 tcctgctgaa gcagaaactg catctgttca agccgtcgcc ctacctgacc ttctccgttc
     3241 atctcgacgg cgaccgcgag cgacacgacc gcagcgtcga ccgtgccggt gtcttcgaca
     3301 aggcggt
//
LOCUS       DASRWS010000444         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1041449, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000444 DASRWS010000000
VERSION     DASRWS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041449"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..2121
                     /locus_tag="VFF72_08395"
     CDS             193..2121
                     /locus_tag="VFF72_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZV93223.1"
                     /translation="MRFLALLLLTLFLAPARAAGGDAPSVETFFSRPKLQSAQLSPSA
                     KWLAITAPAGTGRLSLAVVDAEGKETPRVIASFSNVDVTTFQWVGDDHLVFGVGDLFV
                     GVGDDPTGGIYSIKRDGSQQRRLDHPRRYHFEEVMDIPGGGGDFVILGDFEFNLKGEF
                     DSVSAYRLNVADGTRESLSLNRPGHAKQWLFDPAGNPRLIFAADGGVSTLFWKGSADA
                     PWRQIASYPEFKEAFRPLAVDSSGTLYGLTDEGPARTAVLTRFDFATGKPSSDVIART
                     PGFDFTGQLVFNKDSTLAGLRIVTDAETTAWFDPAMKRVQAAVDKKLAGRVNRVICGD
                     CRSPRVVLVDSFSDRDPGSVWLYRPDADEWHLVGQRRPDIDPAKMAHLDLHRIKARDG
                     MDLPVWITMPPGAPAKSPAVVLVHGGPQVRGFAWQWNPEAQFLASRGYVVIEPEYRGS
                     DGYGRAHVEAGWKHWGDTMQDDNADALAWAVAQGLVDPGRACIAGASYGGYATLMSVI
                     RYPQTYRCGVAWVAVTDPRLLFEESWASDSQEAGRRYYLPLALGDPVKDAEMLRRSAP
                     VERANEIKVPLLLAFGADDQRVPLEHGLRMRAALTASGHPPEWVVYEGEGHGWRSIEH
                     RYDFYRRVEKFLAEQLKH"
     gene            2215..>3618
                     /locus_tag="VFF72_08400"
     CDS             2215..>3618
                     /locus_tag="VFF72_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZV93224.1"
                     /translation="MITLDDLPAIAAFESLPREDLGRLAQASGDVRIGAGDYVVHEGD
                     ERSLFVVLAGRIEVTKVIDGIERVIGVRAPGQIFGEVPITFGTPFQGSYRATEATRVM
                     HISARQFHALAAAHPDVLTHVASKGSERIGGLRGIASAPMTARAVMLGHATDDTVREL
                     RNFLTRNQISYDWVKPDAADRGTRWTGPPVADDELPALMCDDGAVLRRAACRDVAEHL
                     GLQTRPRHDDYDTVIIGGGPAGLAAAVYGASEGLRTLVIEREAPGGQAETSSRIENYL
                     GFPTGVSGDELARRALQQTHRLGAEIAVTRRVTGIDPASKVVTLDGGERVAARTLILA
                     TGVSWRRLPTDGFERLLGKGVFYGVSRSEAGTTQGRDIHLIGAGNSAGQAAMFFANHA
                     RNVTLVVRGSSLEKSMSRYLIEQMRGKPNIAVALNSEVRAAHGDKAITAIDLADRATG
                     ASRRVDSGGVFVFIGADA"
BASE COUNT          592 a         1253 c         1211 g          562 t
ORIGIN      
        1 acgcacacca ccgagaacat cggcggcacc gacgcccacg cctacatcat cgagatcaag
       61 gacgcgcact cgaagccgga gacgggaacc gactgaggtc ggggggaggc ccgggcatcg
      121 atgcgcggcg gcgcctgcct cgccgggacc ggatcgggtc aaatagcggc ttacttccca
      181 tcccggtgac ccatgcgctt cctcgccctt ctcctcctga cactcttcct cgcgccggcc
      241 cgagccgccg gcggcgatgc accttcggtg gagacattct tctcacgtcc caaactgcaa
      301 agcgcgcagc tttcgccttc ggccaagtgg ctggcgatca ccgcgccggc gggcacggga
      361 agactgtcgt tggccgttgt cgatgcggag ggcaaggaga cgccccgggt gatcgcctcg
      421 ttctcgaacg tcgacgtcac gacgttccag tgggtcggcg atgatcacct ggtattcggt
      481 gtcggcgacc ttttcgtcgg cgtcggcgac gatcccaccg gcgggatcta ttcgatcaag
      541 cgcgacggta gccaacagcg acggctcgac catccgcgga ggtatcactt cgaagaagtg
      601 atggacattc cggggggcgg cggcgatttc gtcatcctcg gcgactttga attcaacctc
      661 aagggagagt tcgattcggt cagcgcctac cgcttgaacg tcgcggacgg cacccgggaa
      721 tcgctttcgc tgaaccggcc cgggcatgcg aagcaatggc tcttcgatcc tgccgggaat
      781 ccgcggctga tcttcgcggc cgatggcggc gtctccaccc tgttctggaa gggatccgcc
      841 gatgcgccgt ggcggcagat cgcgtcgtac ccggaattca aggaggcatt ccggccgctc
      901 gctgtcgatt cttcagggac actttatggc ctgaccgacg aaggaccggc acggacggcc
      961 gtcctgacgc gtttcgactt cgcgaccgga aagccgtcga gcgacgtgat cgcacgcacg
     1021 ccgggcttcg atttcacagg ccaactcgtc ttcaacaagg actcgacgct tgccggcctg
     1081 cgaatcgtca ccgacgcgga aacgacggcc tggttcgacc ccgcgatgaa acgcgtgcag
     1141 gccgcggtcg acaagaaact ggcgggacgt gtcaatcgtg tcatttgcgg agactgcaga
     1201 tcgccgcgag tcgtcctcgt cgactcgttt tccgatcggg acccgggcag cgtctggctc
     1261 tatcggccgg acgccgatga atggcatctc gtcgggcagc gtcggcccga catcgatccg
     1321 gccaagatgg cgcacctcga cctgcatcgg atcaaggccc gcgacggcat ggacctcccg
     1381 gtctggatca ccatgccacc gggagcgcct gcgaagtcgc ccgccgtggt gctggttcac
     1441 ggcggcccgc aggtgcgtgg cttcgcgtgg caatggaacc cggaggcgca attcctcgcc
     1501 tcgcgtggtt acgtcgtcat cgagcccgag tatcgcggca gcgacgggta cggccgtgcc
     1561 catgtcgaag ccggctggaa gcactggggc gacaccatgc aggacgacaa cgccgacgct
     1621 ctcgcctggg cggtggcgca gggtctggtc gatcccggcc gggcttgcat tgccggcgca
     1681 agctacggcg gctacgcgac cctgatgagc gtcatccgct acccgcaaac ctatcgctgc
     1741 ggcgtcgcct gggtggcggt gaccgacccg cgactgctgt tcgaagaaag ttgggcgagc
     1801 gattcccagg aggccgggcg gcgctactac ctgccgttgg ccctgggcga tcccgtcaag
     1861 gacgcggaaa tgctgcgccg ctcggcgccc gtcgaacgag cgaacgagat caaggttccg
     1921 ctgttgctcg ccttcggcgc ggacgaccaa cgcgtcccgc tcgagcatgg gcttcgaatg
     1981 cgtgccgccc tcaccgccag cggacacccg cccgagtggg tggtctacga aggcgaaggc
     2041 cacggatggc ggtcgatcga gcatcgctac gacttctatc gtcgggtcga gaagtttctg
     2101 gcggagcaac tcaaacactg agcgacagcc gctttccgcg cgacggctga ccggcacgcc
     2161 tggcgggccg actcgaattg catttcgctg gcagacgcgc cacgatcgcc cgccatgatc
     2221 acgctcgacg atctccccgc gattgccgcc ttcgagtccc ttccgcgcga ggacctgggc
     2281 cgcctggcgc aagcgtcggg cgacgtgcgg atcggcgccg gcgactacgt cgttcacgaa
     2341 ggcgacgagc gatcgctctt cgtcgtcctg gccgggcgca tcgaagtcac caaggtcatc
     2401 gacggcatcg agcgggtgat cggcgtgcgc gcgccgggcc agatcttcgg cgaggtgccg
     2461 atcaccttcg gcacgccctt ccagggcagc taccgcgcca ccgaggcgac gcgggtgatg
     2521 cacatctcgg cgcgccagtt ccacgcgctg gccgcggcgc atcccgacgt cctgacgcac
     2581 gtcgcgtcga agggcagcga acgcatcggc ggcctgcgcg gcatcgcctc cgcgccgatg
     2641 acggcgcgcg ccgtgatgct cggccacgcg acggacgaca cggttcggga gctgcgcaac
     2701 ttcctcaccc gcaaccagat ctcctacgac tgggtcaagc ccgatgcagc cgaccgcggc
     2761 acgcgctgga ccggaccgcc ggtcgccgac gacgagttgc cggcgctgat gtgcgacgac
     2821 ggcgccgtgc tgcgccgcgc cgcctgccgc gacgtcgccg agcacctcgg cctgcagacc
     2881 cggccgcgcc acgacgacta cgacaccgtg atcatcggcg gcggcccggc gggcctggcc
     2941 gccgcggtct acggcgcctc ggaaggattg cgaacgctcg tcatcgagcg cgaggcgccg
     3001 ggcggacagg ccgagacgtc gtcgcggatc gagaactacc tcggctttcc caccggcgtc
     3061 tcgggcgacg agctggcgcg acgtgccctg cagcagaccc atcggctcgg cgcggaaatc
     3121 gccgtcacgc gaagggtgac gggcatcgat ccggccagca aggtcgtcac gctcgacggc
     3181 ggcgagcggg tcgcggcgcg caccctcatc ctcgccaccg gcgtgagctg gcgacgactg
     3241 ccgaccgatg gcttcgagcg gctgctcggc aaaggcgtct tctacggcgt ctcgcgcagc
     3301 gaggccggga cgacgcaggg ccgcgacatc cacctcatcg gcgccggcaa ttccgccggc
     3361 caggcggcga tgttcttcgc caaccacgcg cgcaacgtga cgctggtggt gcgtggcagc
     3421 tcgctcgaga agagcatgtc gcgctacctg atcgagcaga tgcgcggcaa gcccaacatc
     3481 gccgtggcgc tgaacagcga ggtgcgcgcc gcgcacggcg acaaggcgat cacggccatc
     3541 gatctcgccg accgcgccac cggtgcgtcc cgacgcgtcg acagcggcgg cgtcttcgtc
     3601 ttcatcggcg ccgacgcc
//
LOCUS       DASRWS010000445         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3064727, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000445 DASRWS010000000
VERSION     DASRWS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064727"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFF72_08405"
     CDS             <1..1182
                     /locus_tag="VFF72_08405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZV93225.1"
                     /translation="VTAAELQQRIERVREDAARTRTPLPAPAVLRKQVLDTLIDERVL
                     VTNARESGFRVDDAELDRAVASVATQNQLTVPQLRERLRQEGMEFSKFRSNIRDQILV
                     ERVREREVQSRIKISDAEIDAFIEQRRAAAGLSTQYDIAQILVAVPDGASVAEAAQRH
                     ERALTAMRRVRAGEDFATVAREMSDDGNRAKGGEIGMRPADRLPDIFVKAVKSLKAGE
                     IAPDLLRSDAGFHILKLVDRRDSGPFTVQQVHTRHILLRPSAELTVEAAKRRLAQFKQ
                     DVLSGKSTFEQLARANSEDGSAEQGGDLGWTSPGQFVPEFEDAIKDLADGGISDPVVT
                     RFGVHLIQLIGRRQVTLDRQQQREQARNALREQKFEKAYADWLRDLRAEAYIELRDPP
                     Q"
     gene            1212..2000
                     /gene="rsmA"
                     /locus_tag="VFF72_08410"
     CDS             1212..2000
                     /gene="rsmA"
                     /locus_tag="VFF72_08410"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZV93226.1"
                     /translation="MTHRARKRFGQHFLVDQAVVDAIVRAVDPRPGEAIVEIGPGLGA
                     ITKPLLGRCGAVTAIELDRDLAARLTRLEGLELIEADVLRVDFDALAKAQAMKLRIVG
                     NLPYNISTPILFHLLDAIDCVVDQHVMLQREVAERIAALPGGKSYGRLSVMLQWRYAI
                     ETVLDIAPHAFDPPPKVQSAVLRMVPKPALLEVGPSLLGELVTVAFSQRRKLLRHTLG
                     RWLGEHRVDVGFDLQRRAEEVPVEEFVRLAASVERLISGRPAPA"
     gene            complement(2012..2215)
                     /locus_tag="VFF72_08415"
     CDS             complement(2012..2215)
                     /locus_tag="VFF72_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93227.1"
                     /translation="MFLFFSVARSQEIGSWERATEVAPGVNGNNGDKPVKAVAKTAAN
                     SSFGANMPLMSSAFDTAMWLSAA"
     gene            complement(2194..2589)
                     /locus_tag="VFF72_08420"
     CDS             complement(2194..2589)
                     /locus_tag="VFF72_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZV93228.1"
                     /translation="MLLQTVRPNIVQSIRAYRVDDLMQAAKHADQHFLYANLTAAQSK
                     QEVLEGIAEAFLFPTHFGKNLDALFDCMTDLVQKAGAQPGFVVVLEQLPDNPRFDREA
                     REQLLDVFRDAADFWAERKVPFRCFYSFQ"
     gene            complement(2628..3020)
                     /locus_tag="VFF72_08425"
     CDS             complement(2628..3020)
                     /locus_tag="VFF72_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HZV93229.1"
                     /translation="MGRLQPWRPLLRWGAGIGVAGLLAAGSVHAREAVDGSVPLARLP
                     EQAQAVHRAILSGGPFAYPKDGIVFGNREHLLPRRSRGYYREYTVATPGTRDRGARRI
                     VCGGSQPTTPDTCFYSDDHYNSFKRIKP"
     gene            complement(3158..>4430)
                     /locus_tag="VFF72_08430"
     CDS             complement(3158..>4430)
                     /locus_tag="VFF72_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZV93230.1"
                     /translation="TTITAEMEIAAVHAIAELAQAESSEVVTAAYAGATLSFGRDYLI
                     PKPFDPRLMMRIAPAVARAAAESGVAARPIADLDVYRDKLQTFVYASGTAMRPIFSIA
                     KAAKRNRIAFAEGEEERILRAVQVVVDEHLARPTLIGRPDVIAQRIAKFGLRLTAERD
                     YDVVNTEHDQRYRDYWQTYHQLTARKGVTAQLAKIEMRRRLTLIGSMLLRKGEVDGML
                     CGTWGTTAMHLHYIDQVIGKREGTRTYACMNGLILPGRQVMLVDTHVNYDPTAEQLAE
                     ITVMAAEEMMRFGLRPKAALLSHSNYGSSNHPSALKMREALEMLRERAAWLEVDGEIH
                     GDTALDAHYRHEIMPDSPLTGEANLLVLPNIDAANIAYNLLKTAAGGGIAIGPVLLGA
                     GLPAHIMTPSATVRRIINMTALAVADANAAR"
BASE COUNT          744 a         1420 c         1471 g          795 t
ORIGIN      
        1 gtcacggccg ccgagctgca acagcggatc gagcgcgttc gcgaagacgc ggctcgcacg
       61 cgaacgccct tgccggcgcc cgcggtgctg cgcaaacagg ttctcgatac cttgatcgac
      121 gagcgcgttc tcgtcacgaa cgcgcgcgag agcggctttc gcgtcgatga cgccgagctc
      181 gatcgcgccg tggccagcgt cgccacgcaa aaccagctga cggttccgca gctgcgcgag
      241 cgtctgcgtc aggaagggat ggaattcagc aagttccgca gcaacatccg cgaccagatt
      301 ctcgtcgagc gtgttcgcga gcgcgaagtg cagagccgga tcaagatcag cgatgccgag
      361 atcgacgcct tcatcgagca acgtcgcgcc gcggcgggcc tctcgaccca gtacgacatc
      421 gcccagatcc tcgtcgccgt gccggatggc gcgagcgtcg cagaggcagc gcagcggcac
      481 gaacgcgcat tgacggcgat gcgccgggtg cgggcaggcg aggatttcgc gaccgtcgcc
      541 cgcgagatgt ccgatgacgg gaaccgtgcc aagggcggcg agatcggcat gcgtcctgcc
      601 gatcgcctgc ccgacatttt cgtcaaggcc gtgaagtcgc tgaaggcggg cgagatcgct
      661 cctgatctcc tgcgcagcga cgcggggttt cacattctca agctcgtcga tcgccgcgac
      721 tccggtccgt tcacggtgca gcaggtgcac acgcggcaca tcctgcttcg cccgtcggcc
      781 gagctgacgg tcgaggcggc gaaacggcgt ctggcccagt tcaagcagga tgtcctttcg
      841 ggcaagagca cgttcgagca actggcgcgg gccaattccg aggacgggag cgcggagcag
      901 ggcggcgatc tcggctggac gtcgccgggc cagttcgttc cggagttcga ggacgcgatc
      961 aaggacctcg ccgatggcgg gatctccgat ccggtggtga cacgtttcgg cgtgcacctg
     1021 atccagctga tcggtcgccg tcaggtcacc ctcgatcgtc agcagcagcg cgaacaggca
     1081 cgcaacgcgt tgcgcgagca gaagttcgag aaggcttacg ccgactggtt gcgcgacctc
     1141 cgcgccgagg cctacatcga gctgcgcgat ccgccccagt aggtggtgca gcgcgtcgtc
     1201 gcgaacccgc catgacgcat cgcgcgcgca agcgcttcgg ccagcatttc ctcgtcgacc
     1261 aggcggtggt cgacgcgatc gtcagggcgg tcgatccgcg tccgggtgag gcgatcgtcg
     1321 aaatcggccc gggtcttggc gcgatcacga agccgctcct cgggcggtgc ggcgcggtta
     1381 ccgcgatcga gctcgatcgc gacctcgccg cccgcttgac acgcctggag ggcctcgagc
     1441 tgatcgaagc cgacgtcctt cgcgtcgatt tcgatgcact ggcgaaggcc caggcgatga
     1501 agcttcgcat cgtcggcaac ctgccctaca acatctcgac gccgatcctc tttcacctgc
     1561 tggacgcgat cgactgcgtt gtcgatcagc acgtcatgct gcaaagagaa gttgccgagc
     1621 ggatcgcggc gctgcccggt ggcaagtcgt acggccgcct cagcgtgatg ctgcagtggc
     1681 gctatgcaat cgagacggtg ctcgatatcg cgcctcacgc gttcgatccg ccgcccaagg
     1741 tgcaatcggc ggtgcttcgc atggttccga agcccgcttt gctcgaggtc ggaccgtcat
     1801 tgctcggcga gctggtgacg gtggccttct cgcagcggcg caaactgctt cggcacacgc
     1861 tgggtcgttg gctgggcgag caccgggtcg acgtcgggtt cgacctgcag cggcgtgccg
     1921 aggaggtgcc ggtcgaagaa ttcgtccgcc tcgcggcatc cgtggagagg ctgatttcag
     1981 gccggccagc gccagcctga aatcctttcg ctcaggcggc gctaagccac atcgccgtat
     2041 cgaaggcgct gctcatgagc ggcatgttgg caccgaaact gctgttcgcc gctgtcttgg
     2101 cgaccgcctt gacgggcttg tcgccgttgt tgccgttgac tccgggggcc acttccgtcg
     2161 cccgctccca tgaccctatt tcctgggagc gggctactga aaagaataga aacatcggaa
     2221 gggcaccttg cgttcggccc agaagtcggc ggcgtcgcga aagacgtcga ggagctgctc
     2281 gcgggcctca cgatcgaagc gcggattgtc gggaagctgc tcgagcacga ccacgaaccc
     2341 aggctgcgcg ccggccttct gcaccagatc ggtcatgcag tcgaacaggg cgtccaggtt
     2401 cttgccgaag tgggtcggga acaggaacgc ttcggcgatg ccctcgagca cttcctgctt
     2461 cgactgcgcc gccgtcagat tggcataaag gaaatgctga tcggcgtgtt tcgccgcctg
     2521 catcaggtcg tcgacccggt aggcccggat cgactgaacg atgtttggac ggactgtctg
     2581 caagagcatg tctcgttcct cctggtcaaa aaaatgtgcc gcggcgttca tggcttgatt
     2641 cgcttgaagc tgttgtagtg gtcatcggaa tagaagcagg tatccggcgt cgtgggttgg
     2701 gagccgccac agacgatgcg gcgcgcgccc cggtcgcggg tgcccggcgt cgccacggtg
     2761 tattcgcggt agtagccgcg actccgccgc ggaagcaagt gctcacggtt tccaaagacg
     2821 atgccgtcct tgggataggc aaacgggcca cccgaaagga ttgcacggtg tacggcctgg
     2881 gcctgttcgg gaagacgggc cagcggcacc gatccatcca cggcttcccg ggcgtgcacc
     2941 gaaccggctg ccagcaaccc tgcgacgccg atccccgctc cccatcggag cagtggccgc
     3001 cacggctgca gccgacccat cagaaccacg ggaaatccct gaacgttgaa ggcgccgatg
     3061 ttaccgaagc gggccgtaaa gctcaagcct gtgccacata ttggggcacg ttccgcaggg
     3121 attaagtgct tgtttacttt tccttatgcc aagcgtttca gcgggcagcg ttggcatcgg
     3181 ctaccgcaag cgcggtcatg ttgatgatcc ggcgtacggt cgccgaggga gtcatgatgt
     3241 gcgccggcag accggcacca agcagcaccg gaccgatcgc gatgccgccg ccggcggccg
     3301 ttttcaggag gttgtaggca atgttggcgg cgtcgatatt gggcagcacg agcaggttgg
     3361 cctcgccggt cagggggctg tcgggcatga tctcgtgacg atagtgggcg tccagggcgg
     3421 tatcgccgtg gatctcgccg tcgacttcca gccaggcggc ccgctcgcgc agcatttcca
     3481 gcgcctcgcg catcttcagg gccgacgggt ggttgctcga gccgtaattg ctgtgcgaga
     3541 gcagcgccgc cttcgggcgc aggccgaagc gcatcatttc ttcggccgcc atcaccgtga
     3601 tctcggcgag ttgctcggcc gtcggatcgt agttgacgtg cgtatcgacg agcatcacct
     3661 ggcgtccggg caggatgagg ccgttcatgc aggcataggt ccttgtgccc tcccgcttgc
     3721 cgatgacctg gtcgatgtag tgcaaatgca tcgccgtggt tccccacgtc ccgcaaagca
     3781 tgccgtcgac ctcgcccttt cgcagcagca tcgatccgat cagggtcagg cgacgtcgca
     3841 tctcgatctt tgccagctgc gccgtcacgc ccttgcgcgc ggtcaactgg tgataggtct
     3901 gccagtaatc ccgatagcgt tgatcgtgct cggtgttgac gacgtcgtag tcacgctcgg
     3961 ccgtcaggcg caatccgaac ttggcgatcc gctgcgcgat cacgtcgggc cggccgatca
     4021 gcgtcggccg cgccaggtgc tcgtcgacga ccacctgcac ggcgcgcagg atgcgctcct
     4081 cttcgccctc ggcgaaggcg atccggttcc gcttcgccgc cttggcaatg ctgaagatgg
     4141 gacgcatcgc cgtacccgac gcatagacga acgtctgcag cttgtcccga tagacgtcga
     4201 gatcggcgat cggccgggcc gcgacgcccg attcggcggc ggcacgcgcg accgccgggg
     4261 cgatgcgcat catcagacgc gggtcgaacg gcttcgggat gaggtagtcg cggccgaaac
     4321 tgagcgtcgc cccggcgtac gccgccgtca ccacttcgct cgattccgcc tgggcaagct
     4381 cggcgatcgc gtgaaccgca gcgatctcca tttcggcggt gatcgtcgtg
//
LOCUS       DASRWS010000446         6473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1334937, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000446 DASRWS010000000
VERSION     DASRWS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334937"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="VFF72_08435"
     CDS             <1..1983
                     /locus_tag="VFF72_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZV93231.1"
                     /translation="GNPDHAPTNGVLCTKVSRYIERTYHDERVLHPLRRVGAKGSGRF
                     ERVGWDDALDDIAARLKAIAGADGGNAEAIVPYSYCGTMGLLQGEAMAGRFFHRLGAS
                     HLDRTICASAGAEALAATYGAKVGMHVEHFAQSRLIVIWGSNAIASSLHFWAIAQEAR
                     RRGARLVCIDPRRTETAERCDEHIALLPGTDGALALGVMHELVVGGWLDDDYIERHTE
                     GWSALRERALQWPPERTAEVCGIDAQTVRAFARAYGTTKPAAIRLNYGMQRVRGGGNA
                     VRLVAILPCLVGAWRDRAGGLLLSASGWFRGTCDDAALQRPDLLGSRRPRTINMSTIG
                     DDLLRQGGEPLADGRAFGPRIDAVVVYNSNPVAVAPQSPRVAAGFGREDLFTVVLEHF
                     MTDTADLADYVLPATTQLEHLDLHTSYGHTFAVANEPAIAPLGEARPNTRIFAALAKR
                     MGFTEPCFDDSDEDLARLAFRAGGGIDIDALRSQGWSRLEIDEAPFASGGFPTPSGRC
                     RIDAPGLGIPDHVANIESKAVDPERAARFPLAMISPPARHFLNSSFVNVRSLRGIEGE
                     PVLEIAPPDAAARGVVAGQRVRVFNDRGSIVCKAVVGERARPGVVNGLGIWWKKLAAD
                     GRNVNELTHQGLTDIGRAPTFYDCLVEVESAASEAA"
     gene            1980..3074
                     /locus_tag="VFF72_08440"
     CDS             1980..3074
                     /locus_tag="VFF72_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZV93232.1"
                     /translation="MKRAWLVASLGVGGVLATVLAAGVTVCATSGCSTIGYYAQSVTG
                     HLNLVEAARPVSTWLAEPDLDPHLRARLELARQIRDFAVAVLDEPDNQSYRRYADLKR
                     SAAIWNVVAAPELSLQLKTWCFAIVGCVGYRGYYKEASADALGARLRSEGFDVSVYGV
                     PAYSTLGRLPQNGWLADPLLNTFIDYPEGELARLIFHELAHQIAFAPGDTLFNESFAT
                     AVETLGARRWLAERASAQAREQYAQTEGRRADFRALTSRYRDELAALYAGDAPAAVKR
                     EGKASLLARMRADYATMKATRWNGYAGYDRWFAGANNAELGVLASYTGLVPAFERLFE
                     REGSSFPRFYAEVRRLAALPKAERRAALSG"
     gene            3120..3479
                     /locus_tag="VFF72_08445"
     CDS             3120..3479
                     /locus_tag="VFF72_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HZV93233.1"
                     /translation="MADIHIHRTHALGLAKARKTAWKWAEAAEAKFGMECTVIEGESG
                     DVVEFTRPGVDGRLLVAADSFDLTARLGLLVGAFRQRIESEIESNLDALLARGDAVAP
                     ARKGASAKKAATGKRMR"
     gene            complement(3573..3827)
                     /locus_tag="VFF72_08450"
     CDS             complement(3573..3827)
                     /locus_tag="VFF72_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HZV93234.1"
                     /translation="MADLKAQFDQAVIDSKSLPEKPDNMTLLKLYALYKQASGGDADG
                     KRPGFGDMIGRAKWDAWNGLKGKSTDEAMQEYVDLVESLK"
     gene            3937..5505
                     /locus_tag="VFF72_08455"
     CDS             3937..5505
                     /locus_tag="VFF72_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZV93235.1"
                     /translation="MPLATRERMSRVDHAWLRMDNDVNLMMIVGVWLLRPAITHAVLC
                     RRVEERLLQYPRFRQRVSRDAGGATWIDDDDFDLRRHVVRESLPRGRRGGSRERELLQ
                     ARVGELAATPLDSARPLWQFHLIDHYDGGSALVARVHHCIGDGIALISVMLSITDGGA
                     DPPSRGRAASTRRGQPGSDGGSADEGSEDDGAEADGSDDKDGPHGSDASDWLADAVLK
                     PLGGLSARAAGLVEDSIERAIDALADPQRGLAASLAGARTAGKVLGDVTAMASMADDS
                     PTRLKGRPCGRKRVAWCDPIALADVKAIGKAFGCSINDVLLACVAGAIGDWLRHQGDD
                     PAGQEIRAMVPVNLRPLDEAWRLGNRFGLAPLVLPIGIAHPLARVFAVRARMAALKAS
                     YQPLLAFAVLVVAGQMAKPVQDAILGLFAKKATAVMTNVPGPAAPLKFCGSTLEQTMF
                     WVPASGDIGVGVSILSYGGGVQFGLITDEALCAEPQTIVDRFGPRFEELLLLALMLPW
                     EIPRSSRASRLLEA"
     gene            5598..>6473
                     /locus_tag="VFF72_08460"
     CDS             5598..>6473
                     /locus_tag="VFF72_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV93236.1"
                     /translation="MSTLRFRLVAAAALAAGAVAAAPALAQEVVLGASVQLTGPVANT
                     GRYYRDAYQFAIDKINAAGGAKVAGKSYKLALKLYDNQSDVNLSVRQYTQLVSQDKVN
                     LLLGPFASNFALADSAVSEKYQVPMVQGGGASDQIFSRGFKYVFGTLAPASNYFASTI
                     DMLKQLKPAPHTVALLYADDAFDVSVAKGTRPKLKEAGLETTMDERYSTNATDFNSLL
                     SQIKSKNVDVVLVAGHETEILNFIRQAKSLAVSPKIYSFTVGVPSADFRKALGKDADY
                     AFGMTSWLPSATQTDK"
BASE COUNT          962 a         2293 c         2208 g         1010 t
ORIGIN      
        1 ggcaacccgg accatgcgcc cacgaacggc gtgctgtgca ccaaggtctc gcgctacatc
       61 gagcggacgt atcacgacga gcgcgtcctg catccgctgc gccgcgtcgg tgccaagggc
      121 agcggtcgct tcgagcgggt gggctgggac gacgcgctgg acgacatcgc ggcgcgactg
      181 aaggcgatcg ccggcgccga tggtggcaac gccgaggcga tcgtgcccta cagctactgc
      241 ggcaccatgg ggctgctgca gggcgaggcg atggcgggac ggttctttca tcgcctcggc
      301 gcatcgcatc tcgaccgcac gatctgcgcg tcggcgggcg ccgaggcgct cgccgcgacg
      361 tacggcgcca aggtcggcat gcacgtcgag catttcgcgc aaagccgcct gatcgtgatc
      421 tggggcagca acgcgatcgc gagcagcctg cacttctggg cgatcgcgca ggaggccagg
      481 cgacgcggcg cacggctggt ctgcatcgat ccgcgccgca ccgagacggc cgagcgatgc
      541 gacgagcaca tcgccttgct gccgggcacc gacggcgccc tggcgctcgg cgtgatgcac
      601 gagctggtcg tcggcggctg gctcgatgac gactacatcg agcggcacac cgaaggctgg
      661 agcgcgcttc gcgagcgggc gctgcaatgg ccgcccgagc gcaccgccga ggtctgcggc
      721 atcgatgcgc agacggttcg cgcgttcgcc cgcgcctacg ggacgacgaa gccggcggcc
      781 atccgcctca actacggcat gcagcgggtg cgcggcggcg gcaatgcggt ccggctggtg
      841 gcgatcctgc cgtgcctcgt cggcgcctgg cgcgatcgcg ccggcggcct gctgctgtcg
      901 gcgtcgggct ggtttcgcgg cacctgcgac gatgccgcgc tgcagcgccc cgacctgctc
      961 ggctcgcgcc ggccgcgcac gatcaacatg tcgacgatcg gcgacgacct gctgcgccag
     1021 ggcggcgagc cgctcgccga cgggcgcgcg ttcggcccgc ggatcgacgc cgtcgtcgtc
     1081 tacaacagca acccggtcgc ggtcgcgccg cagtcgccgc gagtggccgc cggtttcggc
     1141 cgcgaggatc tcttcacggt cgtgctcgag catttcatga ccgacaccgc cgatctcgcc
     1201 gactacgtgc tgccggcgac gacgcagctc gagcacctcg atctgcacac cagctacggc
     1261 cacaccttcg cggtggccaa cgagccggcg atcgcgccgc tcggcgaggc gaggccgaac
     1321 acgcggatct tcgcggcgct ggccaagcgc atggggttca cggaaccgtg cttcgacgac
     1381 agcgacgaag acctcgcacg gctcgccttt cgcgccggcg gcggcatcga tatcgacgcc
     1441 ctgcgctcgc agggctggtc gcggctcgag atcgacgaag cgccgttcgc tagcggaggc
     1501 tttccgacgc cgagcggccg ctgccgcatc gacgcaccgg gcctcggcat cccggaccac
     1561 gtcgccaaca tcgagtcgaa ggccgtcgat cccgagcgcg ccgcgcgatt tccgctggcg
     1621 atgatctcgc cgccggcgcg ccatttcctg aactcgagct tcgtcaacgt gcgcagcctt
     1681 cgcggcatcg agggcgagcc ggtgctcgag atcgccccgc cggatgccgc cgcgcgcggc
     1741 gtcgtcgccg ggcagcgcgt ccgcgtcttc aacgaccgag gctcgatcgt ctgcaaggcg
     1801 gtggtcggcg aacgggcccg tccgggcgtc gtcaatggtc tgggcatctg gtggaagaag
     1861 ctcgcggccg acggccgcaa cgtcaacgag ctgacccacc aggggctcac cgacatcggt
     1921 cgtgcgccga cgttctacga ctgtctcgtc gaggtcgagt ccgccgcgtc cgaggcggcg
     1981 tgaagcgggc ctggctggtg gcgagcctgg gagtcggcgg tgttctcgcc acggtgctgg
     2041 ccgccggcgt caccgtttgc gccacatcgg gttgcagcac gatcggctac tacgcgcagt
     2101 cggtgaccgg ccacctgaac ctcgtcgagg cggcgcggcc ggtctccacc tggctggccg
     2161 aacccgatct cgatccgcac ctgcgcgcca ggctcgagct cgcccggcag atccgcgact
     2221 tcgccgtcgc cgtgctcgac gagcccgaca accagagcta tcgccgctat gccgacctga
     2281 agcgcagcgc cgcaatatgg aacgtcgtcg ccgcgcccga gctctcgctc cagctgaaga
     2341 cctggtgctt cgcgatcgtc ggctgcgtcg gctaccgcgg ctactacaag gaggccagcg
     2401 ccgacgcgtt gggcgcgcgc ctgcgcagcg agggattcga tgtctcggtc tacggcgtgc
     2461 ctgcgtactc gaccctgggg cggctgccgc aaaacggctg gctcgccgat ccgctgctca
     2521 acaccttcat cgactatccc gaaggcgagc tcgcccgcct gatctttcac gagctcgccc
     2581 atcagatcgc gttcgcgccg ggcgacacgc tcttcaacga gtcgttcgcg acggcggtcg
     2641 agacactggg agcgcggcgc tggctcgccg agcgcgccag cgcacaggcg cgcgagcagt
     2701 acgcgcagac cgaaggccgc cgcgccgact tccgcgccct gacgagccgc tatcgcgacg
     2761 agctcgcggc gctctatgcc ggcgacgcgc ccgcggccgt caagcgcgaa ggcaaggcgt
     2821 cgctgctggc caggatgcgc gccgattacg cgacgatgaa ggcgacgcgc tggaacggct
     2881 acgccggcta cgaccgctgg ttcgccggtg ccaacaatgc cgagctcggc gtgctggcca
     2941 gctacaccgg tctggtgccg gcgttcgagc ggctcttcga gcgcgagggg agcagctttc
     3001 cgcgtttcta tgccgaagtg cggcggctcg ccgccttgcc gaaggccgag cggcgcgccg
     3061 cgctctcggg ctgaacgacg cttgcgatca cgaccgcatc cgacacgagg caatccgcca
     3121 tggccgacat ccacatccac cgcacccacg ccctgggcct cgccaaggcg cgcaagaccg
     3181 cctggaaatg ggccgaggcg gccgaagcga aattcggcat ggaatgcacg gtgatcgaag
     3241 gcgagtcggg cgacgtcgtc gagttcaccc gccctggcgt cgacggccgg ttgctcgtcg
     3301 ctgccgattc gttcgacctg acggcccggc tcggcctcct ggtgggtgcg tttcgccaac
     3361 gcatcgagtc ggagatcgag tccaacctcg acgccctgct cgcgaggggc gacgctgtcg
     3421 cgccggccag gaagggcgcg tccgcgaaga aggctgcgac tgggaaaaga atgcgctgag
     3481 gccagcttcg cccgcaccgg atcgtcaagc cggttgctcc gcgtcgtcgc ttcgccggtt
     3541 cgcgcggccc ggctgccgcg gtgccgctgg acctatttca gcgattcgac gagatctacg
     3601 tattcctgca tcgcctcgtc ggtcgacttg cccttcaggc cgttccacgc gtcccatttg
     3661 gcgcggccga tcatgtcgcc gaagccgggt cgcttgccgt cggcgtcgcc acccgaagcc
     3721 tgcttgtaga gcgcgtagag cttgagcagc gtcatgttgt cgggcttttc aggaagactc
     3781 ttcgagtcga tcacggcctg gtcgaactgt gccttgaggt ctgccatcgt cgtccttcgt
     3841 gatgcggcga gcgggtgaag tcgccaattg acggcgccga tgttaaccag catcttcagt
     3901 gccttcgaaa cacgctgcct gcgggcaggc ttcggcatgc ctctcgcgac gcgcgagcgc
     3961 atgtcgcgcg tcgatcacgc gtggctgcgc atggacaacg acgtcaacct gatgatgatc
     4021 gtcggcgtct ggctgctgcg tccggccatc acccacgcgg tgctttgccg gcgggtcgag
     4081 gagcggctgc tgcagtaccc gcgctttcgc cagcgggtgt cccgcgacgc gggcggcgcc
     4141 acctggatcg acgacgacga cttcgacctg cgtcggcacg tcgtgcgcga atcgttgcct
     4201 cgcggccggc gcggcggcag ccgcgagcgc gagttgctgc aggcgcgcgt cggcgagctc
     4261 gcggcgacgc cgctcgacag tgcgcggccg ctctggcaat tccacctcat cgaccactac
     4321 gacggcggca gcgcgctggt ggcgcgggtc caccattgca tcggcgacgg catcgccctc
     4381 atctcggtga tgctgtccat caccgatggc ggcgccgatc cgccgtcgcg aggaagggcc
     4441 gcttcgaccc gccgcgggca gcccggaagc gatggcgggt cggctgacga agggagtgaa
     4501 gacgacggcg cagaggccga cggcagcgac gacaaggatg gccctcacgg cagcgacgcc
     4561 agcgactggc tggccgacgc cgtgctgaag ccgctcggcg gcctgtcggc cagggccgcc
     4621 ggcctggtcg aagacagcat cgaacgcgcc atcgacgcgc tggccgatcc gcagcgcggc
     4681 ctcgccgcgt cgctggccgg ggcccgcacg gcaggcaagg tgctgggcga cgtgacggcg
     4741 atggcgtcga tggccgacga ttcgccgacg cggctgaagg gccggccctg cggcaggaag
     4801 cgagtcgcct ggtgcgaccc gatcgcgctc gccgacgtga aggcgatcgg caaggcgttc
     4861 ggctgctcga tcaacgacgt gctgctggcg tgcgtcgccg gcgccatcgg cgactggctc
     4921 cgccaccagg gcgacgatcc ggcaggccag gagatccgcg ccatggtgcc ggtcaacctg
     4981 cggccgctcg acgaggcgtg gcggctcggc aaccgattcg gcctggcgcc gctggtgctg
     5041 ccgatcggca tcgcccatcc gctggcccgc gtcttcgccg tgcgggcccg catggccgct
     5101 ttgaaggcga gctatcagcc gctgctcgcg ttcgccgtgc tcgtggtcgc cggccagatg
     5161 gccaagccgg tgcaagacgc catcctcggc ctcttcgcga agaaggccac cgccgtgatg
     5221 acgaacgttc ccggccccgc ggcgccgctg aagttctgcg gctcgacgct ggagcagacc
     5281 atgttctggg tgcccgcctc gggcgacatc ggcgtcggcg tgagcatcct ctcgtacggc
     5341 ggcggcgtgc agttcgggct catcaccgac gaggcgctgt gcgccgagcc gcagacgatc
     5401 gtcgatcgat tcggaccgcg tttcgaggag ctgcttcttc ttgccctcat gctgccctgg
     5461 gaaatccccc ggtcgtcccg ggcaagtcgt ttgctagaag cgtgatgcgt ccacgtcgcc
     5521 cctttctcgc actgccggcg gccacggcac actcccgtcc cggttccttg tccttctttc
     5581 tttttcggag gcccgtcatg tccacccttc gttttcgcct cgtcgccgcc gcagcgctgg
     5641 ccgccggcgc tgtcgccgcc gctcccgcgc tcgcccagga agtcgtcctc ggtgcttcgg
     5701 tgcagctgac cggtccggtc gccaacaccg gccgctacta tcgcgacgcc taccagttcg
     5761 cgatcgacaa gatcaatgcc gccggtggcg ccaaggtcgc gggcaagtcg tacaagctcg
     5821 ccctcaagct ctacgacaac cagtccgacg tcaacctgag cgtgcgccag tacacgcagc
     5881 tcgtctcgca ggacaaggtc aacctgctgc tcggcccctt cgcgagcaac ttcgcgctgg
     5941 ccgattcggc cgtctccgag aagtaccagg tgccgatggt gcagggcggc ggcgcctccg
     6001 accagatctt ctcgcgcggc ttcaagtacg tgttcggcac gctcgcgccg gcgagcaact
     6061 acttcgccag cacgatcgac atgctcaagc agctcaagcc ggcgccgcac acggtggcgc
     6121 tgctctacgc cgacgacgcg ttcgacgtct ccgtcgccaa gggcacgcgg cccaagctga
     6181 aggaagccgg cctcgagacg acgatggacg agcgctacag caccaacgcc accgacttca
     6241 attcgctgct ttcgcagatc aagagcaaga acgtcgatgt cgtgctggtc gccggtcacg
     6301 agaccgagat cctcaacttc atccgccagg cgaagagcct cgccgtcagc ccgaagatct
     6361 acagcttcac cgtgggcgtg ccgagcgcgg acttccgcaa ggcgctcggc aaggatgccg
     6421 actacgcgtt cggcatgacg tcgtggctgc cgtcggcgac gcagaccgac aag
//
LOCUS       DASRWS010000447         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1335433, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000447 DASRWS010000000
VERSION     DASRWS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335433"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..930
                     /locus_tag="VFF72_08465"
     CDS             304..930
                     /locus_tag="VFF72_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZV93237.1"
                     /translation="MTAAVDTRRRVQWVMMGMLMTLLAAFGLSAWAQPAPPTAPGVDG
                     GPPSMGRPMHDGMRHGMHRGRHGHGGPGMMFRGSPEQIGRKIDFMLDGLHATDAQRSQ
                     IKQIAVQAAADLKAQAVAGKGLRQRDMQIFTAPTIDAAAAEQTREQMLQQHDRMSKRA
                     MQAMLDMARVLTPEQRGIIGERIRDRQARMHDRMERMKERMQRQPPQR"
     gene            991..1707
                     /locus_tag="VFF72_08470"
     CDS             991..1707
                     /locus_tag="VFF72_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV93238.1"
                     /translation="MTSRLLLIDDDARLASMVADYLGHSGFEVETAGSLAAGRKHLAG
                     GGYDALVLDLMLPDGDGLDLCRELRGDGRTRQLPLLMLTARGEPMDRIVGLELGADDY
                     LPKPFEPRELLARVKALLRRAAAMPTDDDVLRFGRLEIDLGARVARLDGKACDLTGHQ
                     FDLLVVLAQSPGRVLSRDQIMDALKGHPLEAFDRSIDVHISRIRAVIEDDPKNPRRVL
                     TVRGAGYVFARKQDADDASP"
     gene            1814..>2350
                     /locus_tag="VFF72_08475"
     CDS             1814..>2350
                     /locus_tag="VFF72_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HZV93239.1"
                     /translation="MKSLYLRIYATVVVVLLLFAFASAWILQRHLEQERSRNESVLNE
                     RIGAWADLIEHSLPPADAPADVQVAAMRDWSMRLRVPLALDDASGQRIGASESFLRRS
                     AEGQPARAVPLDDGRTLWTMRPGQRPPGRAGRAGAGRGPGGPVPWPFLPPGVPRSAGL
                     AIILVVLFFAVAAGAYPVV"
BASE COUNT          327 a          869 c          825 g          329 t
ORIGIN      
        1 gtccgtcccc cgggcggggc gcggccgccg ggcggcggcc attctcccgg cgccaggccg
       61 cccgagggca ggccgccgca gggcaggccg cccggaggca ggccggaacc gggacggctg
      121 cccggtcgtc ccgtcggcaa cgagcagcgc tagccgcggc tgcgtgtcgt cgcgaaccgg
      181 ccgggcgacc ggccggttca caattctttg ccccggggtc aagccagctt catggccggt
      241 ccccaacatt gcctgcatcg atccgtagga cgggtccgga gcaatgcgat ggcgagcaag
      301 gcgatgactg cggcggtgga tacccggcgc cgagtgcagt gggtgatgat ggggatgctg
      361 atgacgctcc tcgcggcgtt cggcctgtcg gcgtgggccc agcccgcgcc gccgacggcg
      421 ccgggcgtcg acggcgggcc gccgagcatg gggcgtccga tgcacgacgg catgcgccac
      481 ggcatgcatc gcggccgaca cggccacggc ggtccgggga tgatgtttcg cggctcgccc
      541 gagcagatcg gcaggaagat cgacttcatg ctcgacgggc tgcacgcgac cgatgcgcag
      601 cgcagccaga tcaagcagat tgccgtccag gctgctgccg acctgaaggc acaagccgtc
      661 gccggcaagg gtctgcgcca gcgcgacatg cagatcttca ccgcgcccac catcgacgcc
      721 gcggccgccg aacaaacacg cgagcagatg ctgcagcagc acgaccggat gagcaagcgc
      781 gcgatgcagg cgatgctcga catggcccgg gtgctgacgc ctgagcagcg cggcatcatc
      841 ggcgagcgca tccgcgatcg gcaggcgcgc atgcacgacc gcatggagcg catgaaggag
      901 cgcatgcagc gccagccgcc gcaacgataa tcagacctcg gccgatggtc cgggctccgc
      961 gaggagcccg tcgcgcatcc ggagaacgcc atgacgtctc gcctccttct catcgacgac
     1021 gatgcacgcc tggcctcgat ggtggccgac tacctcggcc attccgggtt cgaggtcgag
     1081 acggcggggt cgctcgctgc cggccgcaag cacctcgccg gcggcggcta cgacgcgctg
     1141 gttctcgacc tcatgctgcc cgacggcgac ggcctcgacc tgtgccgcga gctgcgcggc
     1201 gacggccgca cccggcaact gccgctgctc atgctgacgg cgcgcggcga gccgatggat
     1261 cgcatcgtcg gcctcgagct cggcgccgac gactacctgc ccaagccgtt cgagccgcgc
     1321 gagctcctcg cccgggtcaa ggcgctcctg cgtcgcgccg cggcgatgcc taccgacgac
     1381 gacgtgcttc gcttcggccg cctcgagatc gatctcggtg cgcgcgtcgc ccgcctcgat
     1441 ggcaaggcgt gcgacctgac cggccaccag ttcgacctgc tggtggtgct ggcccagagc
     1501 cccggccggg tgctctcgcg cgaccagatc atggacgctc tcaagggcca tcccctcgag
     1561 gccttcgatc gctcgatcga cgtgcacatc tcgcgcatcc gcgcggtcat cgaggacgat
     1621 ccgaagaacc cgcgccgcgt cctcaccgtg cgcggcgccg gctacgtgtt cgcgagaaag
     1681 caggacgccg acgacgcttc gccatgaggg atgccggccc gccgcgccgc gtcgcgcgcg
     1741 agcccggccg cgccgcgtcc gtcggccatt tcgcaccgca tcgccacctt gagcgctgca
     1801 cggcgctcgc gccatgaaaa gcctgtacct gcggatctac gcgacggtcg tcgtcgtgct
     1861 cctgctgttc gcgttcgcgt cggcctggat cctgcagcgc catctcgagc aggagcgcag
     1921 ccgcaacgag tcggtgctca acgagcggat cggcgcctgg gccgacctga tcgagcattc
     1981 gctgccgccc gccgatgcgc ccgccgacgt gcaggtcgcg gcgatgcgcg actggtcgat
     2041 gcgcctgcgc gtgccgctag ccctcgacga tgcgagcggc cagcgaatcg gcgcctccga
     2101 gtcgttcctg cgtcgctcgg ccgagggcca gcccgcgcgc gcggtcccgc tcgacgacgg
     2161 ccgcaccttg tggaccatgc gcccggggca gcgaccgccg ggccgcgccg gtcgggccgg
     2221 cgcaggccgc ggtcctggag gtccggtgcc ttggccgttc ctgccgcccg gggtaccgcg
     2281 cagtgccggc ctggcgatca tcctggtggt tctcttcttc gccgttgccg ccggcgcgta
     2341 ccccgtcgtc
//
LOCUS       DASRWS010000448         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3367995, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000448 DASRWS010000000
VERSION     DASRWS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367995"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFF72_08480"
     CDS             <1..322
                     /locus_tag="VFF72_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV93240.1"
                     /translation="GNPIRKMAEEFRASYLKVNGTPSTDAFSAYAFDAWLVFANAAER
                     ALKTAEPGTPAFRVALHDAITSTKEVVGTHGVYNFTPDSRYGVDKRAREVVRLDHGKW
                     MLVH"
     gene            407..1462
                     /locus_tag="VFF72_08485"
     CDS             407..1462
                     /locus_tag="VFF72_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93241.1"
                     /translation="MSWDIAFILTTDGLGTGAIYLLAGIGLVLIFSVTRVVFVPFGDV
                     AAFAALTLASFERGILPPTIGLVAILAALSVVAEVGGHLRHGRFALIPRAVAVWGLLP
                     LVPCVLAWAVQRGHAPAALALVASIALVVPITPLLARLTLQPIADASVLVLLIVSLAL
                     HFLLSGLGLLFFGPEGSRTHALVDATWTIADGYSVSGQTLLMVGSALVLSGLFYLFFE
                     RTLTGKALRATAVNRVGARLVGIRPKKTAMLAYTGASLLAGMVGVLISPVTTMYYDSG
                     FILGLKAFIAAIIGGLVSFPLTAIGAVAVGLFESFASFWSGELKDVLVFGLLIPVLLL
                     RSWLAAHAEEDDEEIDE"
     gene            1459..3318
                     /locus_tag="VFF72_08490"
     CDS             1459..3318
                     /locus_tag="VFF72_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZV93242.1"
                     /translation="MSDRGGHVEGPAADRAPAVRAAASGVDGRALRGLGVAFAVVLLL
                     LAPHVLGTFAVSLLNDIGIGALVAIGLVLLTGIGGATSFGQAAFVGVAAYATAWLTTA
                     HGASPWLGLVFALVLTGLAALVIGALTLRLGGHYLPLSTIAWGLSIPLLFGNIEALGS
                     HTGLSGIQPIRVGPWPLLEPRSIYYLVWLMVGAACLFSSNLLRSRPGRAIRSLRGGAT
                     LLASVGADAFRVRLVLFITAALLAGFAGWLYAHLNRFVAPIPFDVRASIEYLLMVVAG
                     GLGQIAGAVIGSGLVLLLKNALQDVLPHIAMQGARLEAVAFSIIFILLLHHARAGLAG
                     FVARWGRRHFPDRIAPVVVDGVKPLPRRAVPARGSELLQVKGAVKRFGGLVAVDHVSF
                     DVRAGEIVGLIGPNGAGKSTMFNLLTGTLRTSAGSVRFAGHDITGMAPQKIARLGVAR
                     TFQHVKLRPRMSLLDNVALGAHARGRAGVLRAGLGLDRREERQIAAEAQRQLERIGLG
                     ARAHELAGSLPLGTQRILEIGRALAADPVLLVLDEPAAGLRRAEKLALAAMLRRLRDE
                     GVTILIVEHDMDFVMKLVDRLIVMDFGAKLVEGAPAEVRADRRVQAAYLGAAA"
     gene            3315..>3615
                     /locus_tag="VFF72_08495"
     CDS             3315..>3615
                     /locus_tag="VFF72_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93243.1"
                     /translation="MSATDEPGGGPATGPHPVVGSAGGAPGSQGDSSTRADLRPLQGS
                     AILPGGRAAAPPAMLEIGDLHVAYGQVESVRGVSLTLGAGQIVSVIGPNGAGKTTL"
BASE COUNT          410 a         1365 c         1253 g          587 t
ORIGIN      
        1 cggcaacccc atccgcaaga tggccgaaga gttccgcgcc tcctacctca aggtcaacgg
       61 cacgccgagc accgacgcct tctcggcgta tgcgttcgac gcctggctgg tcttcgccaa
      121 tgccgccgag cgggcgctga agacggccga gcccggcacg ccggcgtttc gcgtcgcgct
      181 gcacgacgcg atcacgagca ccaaggaagt cgtcggcacg cacggcgtct acaacttcac
      241 gcccgacagc cgctacggcg tcgacaagcg ggctcgcgaa gtggtgcggc tcgaccacgg
      301 caagtggatg ctggtgcact gatcccgacc cgccgccggc cagaatgaca gccctgcggg
      361 cgcccccgcg cgctccgctc cgcgaacccg acgccggggt cgggcgatga gctgggacat
      421 cgccttcatc ctgacgaccg acgggctggg gaccggcgcc atctacctgc tcgccgggat
      481 cggcctggtg ctgatcttct cggtcacccg ggtcgtcttc gttcccttcg gcgacgtcgc
      541 ggcgttcgcg gcgctgactc tcgcgtcgtt cgagcgcggc atcctgccgc cgacgatcgg
      601 cctcgtggcg atcctcgccg cgctctcggt cgtcgccgag gtcgggggtc accttcgcca
      661 tggccggttc gccctgatcc cgcgggccgt cgccgtctgg ggcctgctgc cgctcgtgcc
      721 gtgcgtgctc gcctgggccg tgcagcgcgg ccacgcgccg gcggcgctgg cgctggtcgc
      781 ctcgatcgcc ctcgtcgtgc cgatcacgcc gctgctcgcc cggctgacgc tgcagccgat
      841 cgccgacgcc tcggtgctgg tgctgctgat cgtctcgctg gcgctgcact tcctgctgtc
      901 cggcctcggc cttctcttct tcggccccga aggctcgcgc acccacgcgc tcgtcgacgc
      961 gacgtggacg atcgccgacg gctattcggt cagcggccag acgcttctca tggtcggctc
     1021 ggcgctggtg ctgagcggcc tgttctatct cttcttcgaa cgcaccctca ccggcaaggc
     1081 gctgcgcgcg acggcggtca accgcgtcgg tgcgcgcctt gtcggcatcc ggccgaagaa
     1141 gacggcgatg ctcgcctaca ccggcgcgtc gctgctcgcc ggcatggtcg gcgtgctgat
     1201 ctcgccggtg acgacgatgt actacgactc gggcttcatc ctcggcctga aggcgttcat
     1261 cgccgcgatc atcggcggcc tcgtcagctt tccgctgacc gcgatcggcg ccgtcgccgt
     1321 gggcctcttc gagagcttcg cctcgttctg gagcggcgag ctgaaggacg tgctcgtctt
     1381 cggcctgctc atccccgtcc tcctgctgcg ttcgtggctc gccgcgcacg ccgaggaaga
     1441 cgacgaggag atcgacgaat gagcgatcgc ggcggccacg tcgaaggccc ggccgccgac
     1501 cgcgcgccgg ccgtgcgcgc cgcggcgtcg ggcgtcgacg gccgcgcgct gcgcggcctc
     1561 ggcgtggcgt tcgccgtcgt cctgctgctg cttgcgccgc atgtgctcgg tacgttcgcg
     1621 gtctcgctgc tcaacgacat cggcatcggc gcgctcgtcg ccatcggcct ggtgctgctc
     1681 accggcatcg gcggcgcgac gtcgttcggc caggccgcct tcgtcggcgt cgccgcctac
     1741 gcgacggcgt ggctcacgac cgcccacggc gcttcgccgt ggctgggcct cgtcttcgcg
     1801 ctcgtcctca ccggcctggc ggcgctcgtc atcggcgcgt tgacgctgcg cctgggcggc
     1861 cactacctgc cgctcagcac gatcgcgtgg ggcctgtcga tcccgctgct gttcggcaac
     1921 atcgaggccc tcggcagcca caccggcctg tcgggcatcc agccgatccg cgtcggcccg
     1981 tggccgctgc tcgagccgcg ctcgatctac tacctcgtct ggctcatggt cggcgccgcc
     2041 tgcctgttca gcagcaacct gcttcgctcg cggcccggac gcgccatccg cagcctgcgc
     2101 ggcggtgcga cgctgctcgc cagcgtcggc gccgatgcgt tccgggtccg cctggttctc
     2161 ttcatcaccg cggcgctgct cgccggcttc gccggctggc tctacgcgca cctcaaccgc
     2221 ttcgtcgcgc cgatcccgtt cgacgtgcgc gcgtcgatcg aatacctgct gatggtcgtg
     2281 gccggcggcc tcggccagat cgccggcgcg gtgatcggct cgggactcgt gctgctgctc
     2341 aagaacgcgc tgcaggacgt gctgccgcac atcgcgatgc agggcgcccg cctcgaggcg
     2401 gtggccttca gcatcatctt catcctgctg ctgcaccatg cccgcgccgg gctggccgga
     2461 ttcgtcgcgc gctgggggcg gcgccacttc cccgaccgca tcgcgccggt cgtggtcgac
     2521 ggcgtcaagc cgctgccgcg gcgcgccgtg ccggcccggg gcagcgagct gctgcaggtg
     2581 aagggcgccg tgaagcgttt cggcgggctc gtcgccgtcg accacgtgag cttcgacgtg
     2641 cgcgccggcg agatcgtcgg cctgatcggc ccgaacggcg ccggcaagtc gacgatgttc
     2701 aacctgctga ccggcacctt gcgcacgagc gcgggcagcg tccgttttgc gggccacgac
     2761 atcaccggca tggcgccgca gaagatcgcc cggctcggcg tggcgcgcac gttccagcac
     2821 gtcaagctca ggccgcgcat gagcctgctc gacaacgtcg cgctcggcgc ccacgcgcgc
     2881 ggccgcgccg gcgtcttgcg cgccggcctc ggcctcgacc ggcgcgagga gcggcagatc
     2941 gccgccgagg cgcagcgcca gctcgagcgc atcggcctcg gtgcgcgcgc gcacgagctc
     3001 gcgggcagcc tgccgctcgg cacgcagcgc atcctcgaga tcggtcgtgc cctggccgcc
     3061 gatccggtcc tgctcgtcct cgacgagccg gccgcgggcc tgcgccgcgc cgagaagctg
     3121 gcgctcgcgg cgatgctgcg ccggctgcgc gacgagggcg tgaccatcct catcgtcgag
     3181 cacgacatgg acttcgtcat gaagctcgtc gaccggctga tcgtcatgga tttcggcgcc
     3241 aagctcgtcg agggcgcgcc ggccgaagtg cgtgccgacc ggcgggtgca ggcggcctat
     3301 ctgggagccg cggcgtgagc gcgacggacg agccgggcgg cgggcctgcg accggcccgc
     3361 atccggtcgt gggatcggct ggcggtgcac cgggctcgca gggcgactcg tcgacccggg
     3421 ccgacctgcg tcccctccag ggatcagcca tcctgcccgg cggccgggcg gcagcgccac
     3481 cggccatgct cgagatcggc gacctgcacg tcgcctacgg ccaggtcgag tcggtgcgcg
     3541 gcgtctcgct gaccctcggg gccggacaga tcgtctcggt catcggcccg aacggcgccg
     3601 gcaagacgac gctgc
//
LOCUS       DASRWS010000449         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2457387, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000449 DASRWS010000000
VERSION     DASRWS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457387"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFF72_08500"
     CDS             complement(<1..666)
                     /locus_tag="VFF72_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZV93244.1"
                     /translation="MQSERDWRGWWGLVKKAVSSWVDDYAPSMGAALSYYTVFSLAPL
                     LLIVISIAGLVFGADAARGEIFGQLRGLMGDASAKTVESMLASLNRPASGVIGTVIGV
                     VVLLVGATTVFGELQDALDRIWRAPARSKHGGLWRILRSRLLSFGMILGIAFLLMVSL
                     VLGAVITAFGHWWGNAFVGWEILAQAINLALGFALTTAVFAMIYKLMPRVRVQWHDVW
                     LGAL"
     gene            complement(801..2528)
                     /locus_tag="VFF72_08505"
     CDS             complement(801..2528)
                     /locus_tag="VFF72_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HZV93245.1"
                     /translation="MHPAGRVADPSTSAASASGVDASEQHDVVIAGGGLAGLTLALQL
                     RQAFADIDILVLERRRHPVPEATHKVGESTVEIGADYLSSVLGLKAHLESRQLKKFGF
                     RFFSSEGRRDVDAVTEIGASRYLSVPSYQIDRGIFENFLAEEARRRGITFAPGTLLVD
                     VALADDGGTHRVRFRSDGRVREASTRWFVDACGRAGLLKKKLGLAEPNAHRAGAVWFR
                     MRDRIVIDDWSDDPAWRERCDPAARWLSTNHLVGEGYWVWLIPLSSGSHSVGIVADAA
                     MHPAESMNTFDKAMDWLREHQPRLYDELDPKRDGLQDFAFLRDFSYGCKQVFSGHRWA
                     ITGEAGLFLDPFYSPGTDFIAIANTYITELVGRDRAGRPLDAHAKIYDQIFHSFYEST
                     LALYSGQYAIFGDAQVLPVKVIWDYTYYWGILAQFFFQRRTADLAALSALRAELAHCQ
                     ELNVEVQSLLRRWSAARPPARRANPARMLDQAALPWFSELNRSLRDDLDDAGFRERIR
                     ASTGRMRALAAEIAARARHDDGVVSAPLERLLAGAALPGVPADEIAGPMLFVDADDRS
                     PAGFDAVAG"
     gene            complement(2543..>2878)
                     /locus_tag="VFF72_08510"
     CDS             complement(2543..>2878)
                     /locus_tag="VFF72_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93246.1"
                     /translation="CAADGGPVLLVAFDVQAVGALASVTRSSGLLAAAFVVSPVAGPR
                     SRAAFDLELVGGASVPEALRSDAARSLRGNAMADVLPFAEALALDRSEPLELPLSASL
                     AIRLSPVGL"
BASE COUNT          474 a          991 c         1035 g          378 t
ORIGIN      
        1 cagcgcgccg agccagacgt cgtgccactg cacacgcacg cgaggcatca gcttgtagat
       61 catcgcgaag acagcggtcg tcagcgcgaa gccgagggcg aggttgatcg cctgggcgag
      121 gatctcccag ccgacgaagg cgttgcccca ccagtgtccg aaagccgtga tgacggcgcc
      181 gaggacgagc gacaccatca gcaggaaggc gatgcccagg atcatgccga acgagagcag
      241 gcggctgcgc aggatgcgcc agaggccgcc gtgcttgctg cgcgccggcg cgcgccagat
      301 gcggtcgagc gcatcctgca gctcgccgaa gacggtggtc gcgccgacca gcaggacgac
      361 gacgccgatc acggtgccga tgacgcccga agcgggccgg ttcaggctgg ccagcatgct
      421 ttcgaccgtc ttcgccgaag cgtcgcccat cagcccgcgc aactggccga agatctcgcc
      481 gcgcgccgcg tcggcgccga acaccaggcc ggcgatcgag atgacgatga gcagcagcgg
      541 cgccagcgag aagaccgtgt agtacgagag cgccgctccc atgctgggtg cgtagtcgtc
      601 cacccaagac gagaccgcct tcttcaccag cccccaccag ccccgccagt cgcgttcgct
      661 ttgcatcgag gtcgttcctt ctcgcgtcat cgtcacccgc gattgtcgaa agaggccggc
      721 ggcgcgggca ccgggcgatg ccctttgcgc gtgtaggcgg cggccacccg tgccgcccgc
      781 gtcgtcggtg gcggcctcgc tcagccggcg acggcgtcga atccggcagg gctgcgatcg
      841 tcggcgtcga cgaacagcat cgggccggcg atctcgtcgg ccggcacgcc cggaagggcc
      901 gccccggcga gcagtcgctc gagcggcgcc gagaccacgc cgtcgtcgtg ccgcgcccgc
      961 gcggcaatct cggcagcgag ggcgcgcatc cgcccggtcg aggcccggat gcgctcgcga
     1021 aagccggcgt cgtcgaggtc gtcgcgcagg ctgcggttga gttcgctgaa ccaggggagc
     1081 gccgcctggt cgagcatcct cgccggattg gcacgcctcg ccggcggacg cgccgccgac
     1141 caccggcgca gcagcgactg gacctcgacg ttgagctcct ggcagtgcgc cagctcggcg
     1201 cgcagcgccg acaacgccgc gaggtcggcg gtgcggcgct ggaagaagaa ctgcgccaag
     1261 atgccccagt agtaggtgta gtcccagatc accttcaccg gcagcacctg ggcgtcgccg
     1321 aagatcgcgt actggccgct gtacagcgcc agcgtgctct cgtagaagga gtggaagatc
     1381 tggtcgtaga tcttcgcgtg cgcatcgagc ggccggccgg cccggtcgcg gccgacgagc
     1441 tcggtgatgt aggtgttggc gatggcgatg aaatcggtgc cgggcgaata gaacgggtcg
     1501 aggaacaggc ccgcttcgcc ggtgatggcc caccggtggc cggagaagac ctgcttgcag
     1561 ccgtacgaga aatctcgcag gaaggcgaag tcctgcaggc cgtcgcgctt cgggtcgagc
     1621 tcgtcgtaaa ggcgaggctg gtgctcgcgc agccagtcca tcgccttgtc gaacgtgttc
     1681 atcgactcgg ccgggtgcat ggcggcgtcg gcgacgatgc ccaccgagtg cgatccggaa
     1741 gacagcggga tcagccagac ccagtagcct tcgccgacca ggtgattggt cgagagccag
     1801 cgcgcggccg ggtcgcagcg ctcgcgccag gccggatcgt ccgaccagtc gtcgatgacg
     1861 atgcgatccc tcatgcggaa ccagaccgcg ccggcgcggt gcgcgttggg ctcggccagg
     1921 cccagcttct tcttcagcag gccggcccgg ccgcaggcat cgacgaacca gcgcgtgctc
     1981 gcctcgcgca cgcggccgtc gctgcgaaag cggacccgat gcgtgccgcc gtcgtcggcg
     2041 agagcgacgt cgacgagcag cgtcccgggc gcgaaggtga tgccgcggcg acgcgcctcc
     2101 tcggcaagga agttctcgaa gatgccgcga tcgatctggt agctcggcac cgacagatag
     2161 cggctggcgc cgatctcggt caccgcgtcg acgtcgcgcc ggccttccga cgagaagaag
     2221 cgaaagccga acttcttcaa ttgccgcgat tcgagatgcg ccttcagccc gaggacgctg
     2281 gagaggtagt cggcaccgat ctcgaccgtc gattcgccga ccttgtgggt cgcctcgggc
     2341 acgggatggc gacgccgctc gaggacgagg atgtcgatgt cggcgaaggc ctgccgcaac
     2401 tgcagcgcga gcgtcagtcc ggccaggccg ccgccggcga tgacgacgtc gtgctgctcg
     2461 ctcgcatcga caccgctggc cgatgcggcc gaggtggaag ggtcggcgac gcggccggcg
     2521 ggatgcatgg acgccgcctc gatcagaggc cgaccggcga cagccgtatc gccagcgacg
     2581 ccgagagcgg cagctcgagt ggctcgctgc ggtccagcgc cagcgcctcg gcgaacggca
     2641 gcacatcggc catcgcgttg ccgcgcaggc tgcgcgcggc gtcggagcgc agcgcctcgg
     2701 gcaccgaggc gccgccgacg agctcgagat cgaacgccgc ccggctgcgt ggtccggcga
     2761 cgggcgagac gacgaaggcg gcggcgagca ggcccgagct ccgtgtcacc gaggccagcg
     2821 cgccgaccgc ctgcacgtcg aacgccacca gcagcaccgg gccgccatcg gccgcgca
//
LOCUS       DASRWS010000450         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2760339, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000450 DASRWS010000000
VERSION     DASRWS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760339"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFF72_08515"
     CDS             complement(<1..674)
                     /locus_tag="VFF72_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZV93247.1"
                     /translation="MKLTRRNFSGGLVAGLGWIGAPGPASAQGGGPVEGVNYVRLEQP
                     LPIILPGKIEVVEFFWYECPHCNAFEPALEAWSRRLPPDVVLRRVPVWFREEPFTAQQ
                     KLFYALDSLGLLPTMHRRVFYAIHNEHVRLRTPEEITAFMMKNGVDPVKFLPTYNSFA
                     VQTKARQARALAAAYKIDAVPAMGVQGRFYTNGSLANANRPLTTPEGTNEQMLVVVDA
                     LIDKIRR"
     gene            complement(760..1458)
                     /locus_tag="VFF72_08520"
     CDS             complement(760..1458)
                     /locus_tag="VFF72_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZV93248.1"
                     /translation="MNTSFPRPAAQRGGFVIGLVVGLLVGLALALGVALYVTKAPVPF
                     INKVQPRPPGQDAAEAEHNRNWDPNSSLASKTPVETGAAASAPAVGTAPPTLVPPTVI
                     DPNAVPPAPATTATVPRGERPPVAAAVNPPGAENPTNTARAAPPLMPLAGVDPFTYFV
                     QAGAFARNEDAEQQRAKLAIIGIESRVTEREQNGRTVYRVRVGPFGRKTEADAAKDKL
                     ADAGVDSALVRVQK"
     gene            complement(1455..3161)
                     /gene="argS"
                     /locus_tag="VFF72_08525"
     CDS             complement(1455..3161)
                     /gene="argS"
                     /locus_tag="VFF72_08525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZV93249.1"
                     /translation="MTRAREELLVALRAAMDEVSPGNALPATFETPRQAAHGDLACTA
                     AMPLAKAQRRNPREVAAELIAALRRKTAVEHWVESIDVAGPGFINLHLRAAARQRIVA
                     DILDAGAGYGRKAADARPPVLVEFVSANPTGPLHVGHARQAALGDAICNLLETQGRRV
                     TREFYYNDAGVQIATLAASVEARLRGLAPGDPGWPESAYNGDYIADIAADFAAGKTVR
                     ADDRAFTASGRIDDLDGIRGFAVAYLRHEQDLDLAAFGVRFDNYYLESSLYTSGRVEA
                     TVARLVEAGKTYEHDGALWLRSTDYGDDKDRVMRKSDGSWTYFVPDVAYHLAKFERGF
                     HDAIDIQGTDHHGTIARVRAGLQAAAMGVPETYPRYILNTMVRVERGGQEVKISKRAG
                     GYVTLRDLIEWTSRDAVRFFMLSRKADTEFTFDVDLARKRNDENPVFYVQYAHARICS
                     VIEQHSAQGGDVVRLQDADLARLTAPSETALLKKLADYPEVLSGAAAGLAPHEIAFYL
                     RELAALFHSYYAAERFLVDDAALAQARLALLAATRQVIRNALAVLGVSAPDRMQRDVE
                     PE"
BASE COUNT          446 a         1052 c         1151 g          518 t
ORIGIN      
        1 cgcctgatct tgtcgatcag ggcatcgacg accaccagca tctgctcgtt ggtgccttcg
       61 ggcgtcgtca ggggacgatt ggcgttggcc aggctgccgt tcgtgtagaa gcgaccctgc
      121 acgcccatgg ccggcacggc gtcgatcttg taggcggcgg ccagggctcg cgcctgccgc
      181 gccttcgtct gcaccgcgaa cgagttgtag gtcggcagga atttcaccgg gtcgacgccg
      241 ttcttcatca tgaacgcggt gatctcctcg ggcgtgcgca gccggacgtg ctcgttgtgg
      301 atggcgtaga agacgcgtcg atgcatcgtc ggcagcaggc ccaggctgtc gagggcatag
      361 aacagctttt gctgggcggt gaaaggctct tccctgaacc acaccgggac ccgtcgcaac
      421 acgacgtcgg gcggcagccg ccgcgaccac gcctcgaggg cgggctcgaa agcattgcag
      481 tgcgggcact cgtaccagaa gaactcgacg acctcgatct tgccgggcag gatgatcggc
      541 aggggctgtt caaggcgcac gtagttcacg ccctcgaccg ggccgcctcc ttgcgccgaa
      601 gcggggcccg gggcgccgat ccagccgaga ccggccacca agccaccgga gaaattgcgt
      661 cgggtgagtt tcatgcggtg ccttcgttgc cctttcggac cttcgagagc gagtggcgcc
      721 ggcaagttcg cgccgtcgcg gcggcccctt tccgaacggt cacttctgga cccggacgag
      781 cgccgaatcg acgccggcat cggcgagctt gtccttggcg gcgtcggcct cggtcttgcg
      841 cccgaacggg ccgacacgga cccgatagac ggtgcggccg ttctgctcgc gctcggtgac
      901 cctgctctcg atgccgatga tcgccagctt ggcgcgctgc tgctcggcgt cctcgttgcg
      961 cgcgaaggcg ccggcctgaa cgaagtaggt gaacgggtcg acgcccgcaa gcggcatcag
     1021 cggcggtgcg gcgcgggccg tgttcgtcgg gttctcggca ccaggcggat tgactgcggc
     1081 ggcgaccggc ggccgctcgc cgcgtggcac cgtcgccgtc gtggccggag ccgggggcac
     1141 cgcattcgga tcgatgaccg tcggcggcac gagcgtcggt ggcgcggtgc cgaccgccgg
     1201 tgccgatgcg gcagcgccgg tctcgaccgg cgtcttgctg gccaaggagc tgttcggatc
     1261 ccagttgcgg ttgtgctcgg cctctgccgc gtcctggccg ggcggacgcg gttggacctt
     1321 gttgatgaag ggaaccggcg ccttggtcac gtagagcgcg acgccgaggg ccagtgccag
     1381 gccgaccagc aggccgacga cgaggccgat gacgaagccg ccgcgttgcg cggcgggccg
     1441 cgggaaagaa gtgttcattc cggttcgacg tctcgttgca tgcggtcggg ggcgctgacg
     1501 ccgagcacgg cgagcgcgtt gcggatcacc tgccgcgtcg ccgcgagcaa ggccaggcgc
     1561 gcctgcgcca gcgcggcatc gtcgaccagg aatcgctccg ccgcgtaata gctgtggaac
     1621 aacgcggcca gctcgcgcag gtagaacgcg atctcgtgcg gcgccagacc ggccgcagcg
     1681 ccgctcagga cctcgggata gtcggcgagc tttttcagca gcgcggtctc gctcggcgcg
     1741 gtcagtcgcg ccaggtcggc gtcctgcagc cggacgacgt ctccgccctg ggccgagtgc
     1801 tgctcgatca ccgagcagat cctggcgtgc gcgtattgca cgtagaagac cgggttctcg
     1861 tcgtttcgct tgcgcgccag gtcgacgtcg aaggtgaatt cggtgtcggc cttgcggctc
     1921 agcatgaaaa agcgcaccgc gtcgcggctg gtccactcga tcaggtcgcg cagcgtcacg
     1981 tagccaccgg cgcgcttgga gatcttcacc tcctggccgc cgcgctcgac tcggaccatc
     2041 gtgttcagga tgtatcgcgg ataggtctcg ggcacgccca tcgccgccgc ctgcaggccg
     2101 gcacgcactc gcgcgatcgt gccgtggtgg tcggtgccct ggatgtcgat cgcgtcgtga
     2161 aagccgcgct cgaacttggc caggtggtag gcgacgtcgg gcacgaagta ggtccagctg
     2221 ccgtcggact tgcgcatgac gcggtccttg tcgtcgccgt aatcggtgct gcgcaaccag
     2281 agggcgccgt cgtgctcgta ggtcttgccc gcctcgacca ggcgcgccac cgtcgcctcg
     2341 acccggccgc tggtgtacag gctcgactcg aggtagtagt tgtcgaagcg cacgccgaag
     2401 gcggcgagat cgaggtcctg ctcgtggcgc aggtaggcga cggcgaagcc gcggatgccg
     2461 tcgaggtcgt cgatccggcc ggaggcggtg aacgcgcgat cgtcggcgcg caccgtcttg
     2521 ccggccgcga agtcggcggc gatgtcggca atgtagtcgc cgttgtaggc gctctccggc
     2581 cagcccggat cgcccggcgc caggccgcgc aggcgtgcct cgaccgatgc ggcgagcgtc
     2641 gcgatctgca ccccggcgtc gttgtagtag aactcgcgcg tcacccggcg cccctgcgtc
     2701 tcgagcagat tgcagatcgc gtcgccgagc gctgcctggc gggcatggcc gacatgcagg
     2761 ggaccggtcg ggttggccga gacgaactcg acgaggaccg gcggccgcgc gtcggccgcc
     2821 tttcggccgt agcccgcgcc tgcgtcgagg atgtcggcga cgatgcgctg gcgagccgcg
     2881 gcgcgcaggt gcaggttgat gaaaccgggg ccggcgacgt cgatcgactc gacccagtgc
     2941 tcgaccgccg tcttgcgacg gagcgccgcg atcagctcgg ccgcgacttc gcgcggattg
     3001 cgccgctgcg ccttggccag cggcatcgcc gcggtgcagg caaggtcgcc atgcgccgcc
     3061 tgacgcggtg tctcgaacgt ggcgggaagg gcgttgccgg ggctcacttc gtccatggcc
     3121 gcgcgcagcg cgacgagcaa ttcttcacgg gcccgggtca tcggcga
//
LOCUS       DASRWS010000451         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3919136, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000451 DASRWS010000000
VERSION     DASRWS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919136"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFF72_08530"
     CDS             <1..492
                     /locus_tag="VFF72_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZV93250.1"
                     /translation="WRPEFVAVEERSATVHGWHRALEMHSRINRGTPDCPGLVFALVA
                     GGSCRGRAYRIERSRVDAELRRLWCREMPTGVYDPRWLPCRTPQGVVRALAFTLSRHS
                     PSHTGTLPDEKMVEILRTANGRYGSTLTYLVETATTLRSCGIRDRDIERLVALARRHA
                     LTT"
     gene            complement(482..1117)
                     /locus_tag="VFF72_08535"
     CDS             complement(482..1117)
                     /locus_tag="VFF72_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZV93251.1"
                     /translation="MSNVCTVVVLAAGKGSRFMADSHKLVAPFGDSGVLATTLRQAIA
                     SQLHVVVVTTAAFAELARQSIAARDVVVLPEVGSDGAGELGMGHSIAAGVSAQPNAGG
                     WLILPGDMPLIRPSTLQAVARALDDHPIAFAQHGGMRGHPVGFAAELYSELLGLEGDE
                     GARRLVARYPALGIELDDPGVLVDIDTTEDLASARQETQARPRAVAPDLRS"
     gene            1321..1956
                     /gene="pdxH"
                     /locus_tag="VFF72_08540"
     CDS             1321..1956
                     /gene="pdxH"
                     /locus_tag="VFF72_08540"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZV93252.1"
                     /translation="MTDIATLRKSYELAELDEDASEAEPIRQFAAWFEQALAAKVPEP
                     NAMTLATVGADGRPSSRIVLVKGFDERGLVWFTNYDSRKGRELAIHPFAALQFHWVEL
                     ERVVRIEGPVERTDAAESDAYFASRPLDSRIGAWASPQSEVIASRAVLVANAARFGAR
                     FMLRPPRPPHWGGFRLRPEAWEFWQGRKSRLHDRLRYRREGDAWIRERLAP"
     gene            2141..2950
                     /locus_tag="VFF72_08545"
     CDS             2141..2950
                     /locus_tag="VFF72_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZV93253.1"
                     /translation="MNSLFVTLGIAAWKPVIAALILPPVPFLLLTLIGARLLLSRRGL
                     GWLLIVISVVLLWLTACSGAARELSHLMLQPPAALSFDRVRELRADVAAKKPVAIVIL
                     GGGAEPFAPEYGVSSLQDQSLQRLRYGLWLARQTGAPVAYSGGAGWGQPDVTPEARIA
                     AQIAADDFRQPIKWIEDDSRDTRENATRTMALLKAAGIDHVVLVTHGYHMARALRDFR
                     EAAGAGVTIEPAPMGLAKRLESPSLEWMPSGRGFLRMREVLREIVGKAAGA"
     gene            complement(3061..3339)
                     /locus_tag="VFF72_08550"
     CDS             complement(3061..3339)
                     /locus_tag="VFF72_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93254.1"
                     /translation="MRVFTAMIVVALSACGTKSDVIPTGKSTYLVRSPKTGGGPAGDA
                     EIKAYGIRRANEFCDAEGKHAIVNIGETGGLQLFNVQRAEVRFSCVDK"
BASE COUNT          541 a         1218 c         1169 g          526 t
ORIGIN      
        1 tggaggccgg aattcgttgc cgtcgaggaa cgcagcgcca ccgttcacgg ctggcaccgg
       61 gcgctcgaga tgcactcgcg catcaatcgc ggcacgcccg attgccccgg cctcgtcttc
      121 gcgctcgtcg ctggcggctc gtgtcgcggc cgcgcctatc ggatcgagcg gtcgcgggtc
      181 gacgcggagt tgcggcgcct ctggtgtcgc gagatgccca ccggcgtcta tgacccgcgc
      241 tggcttccct gccgcacccc gcaaggcgtc gtgcgggcgc tggccttcac cctgagccgc
      301 cacagcccga gtcataccgg aacgctgccc gacgagaaga tggtcgagat cctgcgcacg
      361 gccaacgggc gctacggcag tacgctgacc tacctcgtcg agacggcgac cacccttcga
      421 agctgcggca tccgcgaccg cgacatcgaa cgcctggtgg cgctcgcccg ccggcacgcc
      481 ctcacgacct gaggtcgggc gccacggcgc gcggccgggc ctgggtttcc tgccgtgccg
      541 aagcgaggtc ttcggtggtg tcgatgtcga ccaggacacc cggatcgtcg agctcgatgc
      601 cgagcgccgg gtagcgcgcg accaggcgcc gcgccccttc gtcgccctcc aggcccagca
      661 gctccgaata gagctcggcg gcgaaaccga ccggatgccc acgcattccg ccgtgctggg
      721 caaaggcaat gggatggtcg tcgagggcgc gtgccaccgc ctgcagggtc gacggccgga
      781 tcagcggcat gtcgccaggc aggatcagcc agccgccggc gttgggctgg gcgctgacgc
      841 cggccgcgat cgagtgcccc atgccaagct cgcccgcgcc atcgctgccg acttccggca
      901 gaacgacgac gtcgcgcgcc gcgatgctct gccgcgccag ctcggcgaac gccgctgtcg
      961 tgacgacgac gacgtgcagc tggctggcga tcgcctgacg cagggtcgtc gcgagcacgc
     1021 cggaatcccc gaacggcgcg acgagcttgt gggaatcggc catgaagcga gaccccttgc
     1081 ccgcggcaag gacgacgacg gtgcacacgt tggacatgtg tcgaggatgg aagagcgcgg
     1141 cgaaaacgac cagaggaggt ctacgtaagg ccttccccgt aagccggtct gcgtggcgat
     1201 cggggccggc ggcaaacggc gcgcccgacc ggcggagcat gcaacgcagc cgacctcgac
     1261 atcgaatcac ccgtttgtca tcgagggcgc accgttgcgg cgcagttcct atactgcgtc
     1321 gtgaccgaca tcgccaccct tcgcaagagc tacgagctcg ccgagctcga cgaggacgct
     1381 tccgaggccg aaccgatccg ccagttcgcg gcgtggttcg agcaggcact ggcggccaag
     1441 gtccccgagc ccaatgccat gaccctcgcc acggtcggcg ccgacggtcg gccttcgtcg
     1501 cgaatcgtcc tggtcaaggg tttcgacgag cgtggcctgg tctggttcac gaactacgac
     1561 agtcgcaagg gacgcgagct tgcgatccat cccttcgccg ccctgcagtt ccactgggtc
     1621 gagctcgagc gagtcgttcg catcgaaggt ccggtcgaga ggaccgacgc cgccgagtcg
     1681 gacgcctact tcgcgtcgcg cccgctcgat tcgcggatcg gtgcctgggc ttccccccaa
     1741 agcgaagtca tcgcgtcccg ggcggtcctg gtcgcgaacg cggcacgatt cggcgcccgc
     1801 ttcatgctgc ggccgccgcg gccgccgcac tggggcggct ttcgcctgcg acccgaagcc
     1861 tgggagttct ggcaaggccg aaagtcgcgg ctgcacgacc ggctgcgcta tcgccgcgaa
     1921 ggcgacgcct ggatacgcga acgcctggct ccctgactgc gcgctgacgt ctcatccacc
     1981 gggaagaaac gcccgcgacg acctcgccga ccgagcgacc gagcgaccga gcgaccgagc
     2041 gacagcgaga gtgtgacgct ttgcaagcga cccgccgacg tgtccgccgc tcggcacaat
     2101 cccgggttgc tcgaacaaca ggcgaagcgc acaccggatc gtgaacagtc tattcgtgac
     2161 gctcggcatc gcggcgtgga agccggtgat cgccgcgctg atcctgccgc ccgttccttt
     2221 cctgctgctg acgctcatcg gcgcccgcct cctgttgtcc cgccggggac tggggtggct
     2281 gctcatcgtc atcagcgtcg tcctgctctg gctcaccgcc tgctcgggtg cggcacgtga
     2341 gttgtcgcat ctcatgctgc agccgccggc ggccctgagc ttcgatcgcg ttcgcgagct
     2401 tcgcgccgac gtggcggcga aaaagccggt cgccatcgtc atcctcggcg gcggtgccga
     2461 gccgttcgcg ccggaatacg gcgtcagcag cctgcaggat cagtcgctgc agcgcctgcg
     2521 ctacggcttg tggctggctc gccagaccgg cgcgccggtc gcctacagcg gcggcgccgg
     2581 ctggggccag cccgacgtga cgcccgaagc gaggatcgcc gcccagatcg ccgccgacga
     2641 cttcaggcag ccgatcaagt ggatagagga cgactcgcgg gatacccgcg agaacgcgac
     2701 ccgcacgatg gcgctgctca aggccgcggg catcgatcac gtcgtcctcg tcacccacgg
     2761 atatcacatg gcgcgggcgc tgcgcgactt cagggaagct gccggcgcgg gcgtgaccat
     2821 cgagccggcg ccgatgggcc tggccaagcg cctcgaaagt ccgtcgctcg agtggatgcc
     2881 cagcggccgc ggcttcctgc ggatgcgcga ggtcttgcgc gagatcgtcg gcaaggccgc
     2941 cggcgcgtag ggtccggcgc cgccgtccca ggaagaactt gcaccgccat cacgccagcc
     3001 gtcgggtgac gagggtgcgc gagtcggcac cgtcagcgac ggtgccctgt cgtcttcgag
     3061 ctacttgtcg acgcagctga agcgcacttc ggcgcgctgg acattgaaga gctgcaagcc
     3121 gcctgtctct ccgatgttga cgatggcgtg ctttccctcg gcatcgcaga actcgttggc
     3181 gcggcggatg ccgtaggcct tgatctcggc gtcaccggcg gggccgccgc cggttttcgg
     3241 cgaacggacc aggtaggtcg acttgccggt cgggatgaca tcggacttcg tgccgcaggc
     3301 agacagcgcc acaacgatca tcgcggtaaa gactctcatc gcttccttcg ggccagcgcc
     3361 tgactcgacg cgaatgccga agcgtagtgc gtccacccgc cgacgttgaa agcacctgca
     3421 accggcggcg tgacgcatgc cacgacccct accg
//
LOCUS       DASRWS010000452         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1045380, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000452 DASRWS010000000
VERSION     DASRWS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045380"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFF72_08555"
     CDS             <1..975
                     /locus_tag="VFF72_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HZV93255.1"
                     /translation="EVLVRIERCGLCHSDLHIQDGYADLGGGKRLDTTRGMTLPFTLG
                     HEIAGVVEEVGPDAPKDLIGKKQAVFPWIGCGHCRDCANGDENLCVKQRFVGVSIDGG
                     FASHVLVPDAKYLLDYDPLPVNQAATLMCSGVTAYGALKRLVDRPRQRNLLLIGLGGV
                     GQMGLSFAQAMFKQKISVADLNAAARETALRNGASVAYDPAEPDIVRRILKETEGGFD
                     GVIDFAGNEKSMAFAVSALARGGKIVVSGLMGGNFSLPMVQWVYKRMTIEGFMVGTLA
                     EGHELMALARAGKIKPTPMREEPMADAQKWIDELRAGTVVGRVILKSE"
     gene            2058..2357
                     /locus_tag="VFF72_08560"
     CDS             2058..2357
                     /locus_tag="VFF72_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93256.1"
                     /translation="MLLIVILSALALAGLTASVVYRLSRSRQIRIDVRQRRAAIWEGV
                     DEAPQPPWAEPVTEETAPRPHAVHSMASTTVPQLRHQKIEELLAQLVKQAQQSDA"
BASE COUNT          482 a          795 c          753 g          404 t
ORIGIN      
        1 gaagtcctcg ttcgcatcga acgttgcggg ttgtgtcatt cagacctgca tatccaggac
       61 ggatacgccg atcttggcgg cggcaagcgg ctcgacacca cacgcggcat gacgctgccg
      121 ttcacgctgg gacatgagat cgcgggcgtc gtcgaggaag tcggtccaga tgctccaaag
      181 gacctgatcg gaaagaagca ggcggtattc ccctggatcg gctgcggcca ctgccgcgac
      241 tgcgcgaacg gcgatgaaaa cctctgcgtc aagcagcgct tcgtcggcgt ctccatcgac
      301 ggcggttttg ccagccatgt gctggtgcct gatgcgaaat atctgctcga ctacgatcca
      361 ctcccggtga accaggccgc cacgctgatg tgctcgggcg tcactgccta tggggcgcta
      421 aagcgcctgg tcgaccggcc gcggcagcgt aacctcttgc tgatcgggct cggcggcgtt
      481 gggcagatgg gactgtcgtt tgcgcaagcc atgttcaagc agaagatttc ggtagccgac
      541 cttaatgcgg ccgcgcgcga aaccgcgctg cgaaacggcg cgtccgtcgc ctacgacccg
      601 gccgagcccg acatcgtcag acgcatcctg aaggagaccg aaggcggctt tgacggagtg
      661 atcgactttg ccggcaacga gaagtcgatg gcgtttgcgg tctcggcgct ggcccgcggc
      721 ggcaagatcg tcgtatccgg attgatgggc ggcaatttca gcctcccgat ggtccaatgg
      781 gtctacaagc gcatgacgat cgagggcttt atggtcggca cgctggccga gggccacgag
      841 ctgatggcgt tggcccgcgc cggcaagatc aagccgacgc cgatgcgcga agaaccgatg
      901 gccgacgcgc agaagtggat cgatgaattg cgagccggaa cagtggtggg acgcgtgatc
      961 ctcaaaagcg aataacgata ggcagaacct tcgcgtcggc ctccattgcg gacaaccgtc
     1021 ctttctattc tcgtcgccgc cgaccgtcac gcttgcgccg gacagcacaa gcggcgcatc
     1081 ggccaatgat cgaagcagcg gccggcagca acggtctcat cgtaaccggc gccggccatc
     1141 tttcgggcgc ggcggctccc ggacatttcg acaatccgat cgctgcaccg ggcgccagac
     1201 cgcggtaaat gcctggctga aatcgcgtct ttcagcagga aaaacgagcc cgaattaagc
     1261 ctcatcctct ccggaattgc ggcctattgc cacccaagtc tttgacgaca ttcgggatgg
     1321 gtagagcgtc agttagagtc cgagtggagt tataaatggc catcagagcc agcaaattcg
     1381 cgtcagccgt cgccgtcggg cttttcgcca gcatgccatt ggcgatagcc caacctgcgg
     1441 cgacagccca gcctgcggcg acagccaagc ctgagacaac cgcgccagac tgcctcaccg
     1501 cgccgcgcca gggaactcca cagggtcaac attggtacta ccatacggaa cgcggcacca
     1561 aacgccattg ctggtatctg cgcgaggaag gcggacaagt ctcgcacgca gcatcatcgg
     1621 acaaccattc accaggagtg gcagatgcgt ccgcaggaca ggccgagcct gcttcagcca
     1681 cggttgcgga cgctcgggcc gaattccccg agccacagcc ggccgctcag gacaacgccg
     1741 gcaatgctcc tgcacaggct gcacccgcag gcacgcccag ccccgcacct gccacaagca
     1801 gcccgtttcc ggatccatcg caagcggtga gcggagcgag ttcgccaatc gtctcgcgct
     1861 ggcctgcgcc gaacgtcaca gattcggccg cagcgccgcc accacaagcc gccgccaact
     1921 cgaccgacgc aggaccgaat tcgcagcccg cgacgccggc gatagcgccg gccccatctg
     1981 ccgatggatt ggccgccaag gatcccgccc cgccttccgc ttccaacgaa cccgcctcat
     2041 cttccgcttc gctggagatg ctgctgatcg tgatattgag cgcgctggcg ctggcgggcc
     2101 tgacggcgag cgtcgtttat cggctcagcc gcagccggca aatccgcatc gatgtgcgcc
     2161 agcgccgtgc cgcgatctgg gagggtgtcg acgaggcgcc gcaaccgccc tgggccgaac
     2221 cggtcaccga agaaacagcg ccgcgtcctc atgctgtgca cagcatggcc tcgacgaccg
     2281 ttccacagct gcgacatcag aaaatcgaag agctcctggc gcagctggtg aagcaggcgc
     2341 agcaaagcga cgcttgacgc ttgacgcttg cgcaatatcg ttatttctcg agctgccgct
     2401 gcagctcgcg gacgaatgtc gtgaggccga cgcg
//
LOCUS       DASRWS010000453         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1631969, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000453 DASRWS010000000
VERSION     DASRWS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631969"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1208
                     /locus_tag="VFF72_08565"
     CDS             102..1208
                     /locus_tag="VFF72_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HZV93257.1"
                     /translation="MKTRAAVAWKAGEPLTIETVDLEGPRAGELLVEVKATGICHTDW
                     FTLSGDDPEGLFPAILGHEGAGIVVETGPGVTSVRRDDHVIPLYTPECRHCKFCLSRK
                     TNLCQQIRSTQGKGLMPDATSRFSMGGKPILHYMGTSTFANFTVLPEIALAKIRPDAP
                     FDTVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQGARMVGANRIIGVD
                     LNPGREAMARKFGMTDFVNPKDVGNVVDAIVQLTDGGADYSFECIGNTATMRQALECC
                     HKGWGQSIIIGVAPAGAEISTRPFQLVTGRQWKGSAFGGARGRTDVPKIVDWYMDGKI
                     AIDELITHRLELEDINEGFALMKRGESIRSVVLY"
     gene            1311..>2217
                     /locus_tag="VFF72_08570"
     CDS             1311..>2217
                     /locus_tag="VFF72_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZV93258.1"
                     /translation="MNEAAPAAPEPCGSWPSPIDAASVAAASIRFGQTAIRDGAIFWT
                     ESRPAEAGRSVLVRATAGDETADLTPPPFNVRTRAHEYGGGAFVLLDDHGLAFCDDAD
                     QQVHLLAPGRPTRRLTELPAQRFADLCHDRRRGRLLAVREDHREGDIDAVATIVAIDL
                     ASGAQRVIAEGHDFFSNPTLSPDGSRLAWLSWDHPDMPWDATTLWLATVGADGSLGEP
                     RRIAGGGSESVFQPSWSSSGELHFVADRSGWWNLHRLRDDAVEPLWPMAAEFGEPQWV
                     FGMSTYGFDGRGRIVCSFLQDAWSTL"
BASE COUNT          351 a          800 c          749 g          317 t
ORIGIN      
        1 cgaccaaggc ggtcgacacg atccgcgccg atgcgcggcc gtcgctcgcg ccgccgctca
       61 ccgtccccga cgttcaccga ctcgagcata ggaacaaggc catgaagacg cgcgccgcgg
      121 tcgcctggaa ggcaggcgag ccgctcacga tcgagaccgt cgacctcgaa gggccgcgcg
      181 ccggtgagct gctcgtcgag gtcaaggcga ccggcatctg ccacaccgac tggttcacgc
      241 tctcgggcga cgaccccgag ggcctgttcc cggcgatcct cggccacgaa ggcgccggca
      301 tcgtcgtcga gaccgggccg ggcgtgacga gcgtgcgccg cgacgaccac gtgattccgc
      361 tctacacgcc ggagtgccgg cactgcaagt tctgcctgtc gagaaagacc aatctctgcc
      421 agcagatccg cagtacccag ggcaagggac tgatgcccga tgcgacgtcg cgcttctcga
      481 tgggcggcaa gccgatcctg cactacatgg gcaccagcac cttcgccaac ttcaccgtgc
      541 tgccggagat cgcgctggcg aagatccgcc ccgacgcgcc gttcgacacg gtctgctaca
      601 tcggctgcgg cgtcaccacc ggcgtcggcg cggtcctctt cacggccaag gtcgaggccg
      661 gcgccaacgt cgtcgtcttc ggcctgggcg gcatcggcct caacgtcatc cagggcgccc
      721 ggatggtcgg cgcgaacagg atcatcggcg tcgacctgaa ccccggccgc gaagcgatgg
      781 cgaggaaatt cggcatgacc gacttcgtca acccgaagga cgtcggcaac gtcgtcgacg
      841 ccatcgtcca gctcaccgat ggcggtgccg actacagctt cgagtgcatc ggcaacaccg
      901 cgacgatgcg ccaggcgctc gagtgctgcc acaagggctg gggccagtcg atcatcatcg
      961 gcgtcgcgcc ggccggcgcc gaaatctcga cccggccgtt ccagctcgtc accggccggc
     1021 aatggaaagg ctcggccttc ggcggcgcgc gcggccgcac cgacgtgccg aagatcgtcg
     1081 actggtacat ggacggcaag atcgccatcg acgagctcat cacgcaccgc ctcgagctcg
     1141 aagacatcaa cgaaggcttc gcgctcatga agcgcggcga gtcgatccgt tcggtggtgc
     1201 tgtactagct tcgtcggagt ggtctgcacg ccgcgatggc gcgccggact gagcaccgtg
     1261 cgagcggcgg gcgctatcct gccggccatg acaaccgggc gacgatgccg atgaacgaag
     1321 ccgccccggc cgcgccggag ccctgcggca gctggccttc gccgatcgat gcggccagcg
     1381 tcgccgcggc gtcgatccgc ttcggccaga cggcgatccg cgacggcgcc atcttctgga
     1441 ccgagtcacg tccagcagaa gcgggacgca gcgtgctggt ccgggcgaca gcgggcgacg
     1501 aaacggccga cctgacgccg ccgccattca acgtccgcac gcgtgcccac gagtacggcg
     1561 gcggcgcctt cgtgctgctc gacgaccacg gcctcgcctt ctgcgacgac gccgaccagc
     1621 aggtccacct tctcgcgccc ggccggccga cccggcgcct caccgaactg ccggcgcagc
     1681 gcttcgccga cctctgccac gaccggcgcc gcggccgcct gctcgcggtg cgcgaggatc
     1741 atcgcgaggg cgacatcgac gcggtggcga cgatcgtcgc gatcgacctg gcgtcgggcg
     1801 cccagcgcgt catcgccgag ggccacgact tcttctcgaa cccgactctg tcgcccgacg
     1861 gcagccggct cgcctggctg agctgggacc atcccgacat gccgtgggac gcgacgacgc
     1921 tctggctggc gaccgtcggt gccgacggca gcctcggcga gccgcgacgg atcgccggcg
     1981 gcggcagtga gtcggtgttc cagccgagct ggtcgtcgtc gggagaactg catttcgtcg
     2041 ccgaccgcag cggctggtgg aacctgcatc gcctgcgcga cgacgcggtc gagccgcttt
     2101 ggccgatggc cgccgaattc ggcgagccgc agtgggtgtt cggcatgtcg acgtacggtt
     2161 tcgacggtcg cggccgcatc gtctgcagct tcctgcagga cgcctggtcg acgctgg
//
LOCUS       DASRWS010000454         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3371249, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000454 DASRWS010000000
VERSION     DASRWS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3371249"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFF72_08575"
     CDS             complement(<1..726)
                     /locus_tag="VFF72_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZV93259.1"
                     /translation="MTLPPQSPTRRHLLAAGLGTAAGLGALGRPALAQTTYEQPFANG
                     ERRMAVYPEKRSLIVITSRPPQLETPMEVFNQGLLTPNDAFFVRYHNAGIPTSIDADT
                     HVVRIGGSAAGKPLALRLADLKSQFRPVETVAVNQCSGNSRGFFDPRVTGGQSGNGTM
                     GNARWLGVPLKDVLARAEVKPSARQVTFDGLDNALFGGGDFVKAIDIHDAMNPDILIA
                     WQMNGADLPMLNGYPVKLVVPGFY"
     gene            976..2244
                     /locus_tag="VFF72_08580"
     CDS             976..2244
                     /locus_tag="VFF72_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZV93260.1"
                     /translation="MKSSSSSSTRAVARSLAGAAIAALAMTAAHAASDVRVLLGVDPA
                     DTTGDVLLSASLGPAQSLGRALGSRVTITQTSRMFDVMRASRTVENDIIIAPAHVTAS
                     AILHEYQLLATSGQDQVYVLMAKKGIDSVDKLPHKRLYLPQQDSLRSYVAKGLLTDSG
                     VKLNQFSKVTYGNTSGAGLVALSFDMADVTITDEAQAKQWIATHPGGAHILKTTRPVP
                     GGMSIVVRKDFCVTECARIAEWINSPDGVIPGIGRFRIASADAGKQFTYVASLGIATP
                     EGVKGATRVSADQVADLMKQGVTLVDTRTQKEYDSEHVRGAILAPYGEKSLKEIDFDA
                     SKDDYHAIDALPKDKPLILMCNGTECWKSYKATRHAVVAGFKTIYWFRGGMPEWREKR
                     LPVDGTAGASMARTTAPAKSGGRAVVAASH"
BASE COUNT          407 a          857 c          871 g          391 t
ORIGIN      
        1 atagaagccc ggaacgacca gcttgaccgg atagccgttg agcatcggca ggtcggcccc
       61 gttcatttgc caggcgatca ggatgtccgg gttcatcgcg tcgtggatgt cgatcgcctt
      121 gacgaagtcg ccgccgccga acagggcgtt gtcgaggccg tcgaaggtga cttgcctggc
      181 cgatggcttg acctcggcac gggcgagcac gtccttcaac ggcacgccga gccagcgtgc
      241 attgcccatc gtgccgttgc ccgactggcc gccggtgacg cggggatcga agaagccgcg
      301 gctgttgccg gagcactgat tgacggcgac ggtctcgacc ggccggaact gcgatttcag
      361 atcggccagg cgcagcgcca gcggcttgcc ggccgcgctt ccgccgatcc tgacgacatg
      421 cgtgtcggcg tcgatcgagg tcggtatgcc ggcgttgtga tagcggacga agaaggcgtc
      481 gttgggcgtc agcagcccct ggttgaagac ctccatcggt gtttcgagtt gcggtggccg
      541 cgacgtgatc acgatcaacg accgcttctc ggggtacacc gccattcgcc tctcgccgtt
      601 ggcgaacggc tgctcgtacg tcgtctgggc cagcgccggg cggccgaggg cgcccaggcc
      661 ggcggcggtt ccgaggccgg cggcaaggag atggcgtcgc gtcggcgact gcgggggcaa
      721 ggtcacgatg tcgtcctttc ggtgaggtgt cgaaaacgcg gcgcaggtga acgggcgcgc
      781 gtgcggattg tgatggcgaa gaccggatcg gcgccagcgc tgcacccgtc gccgcagcgc
      841 cggctctgcg ccggacaggc accttctgtg gcttcgttgc catagccgtg aagtagtttt
      901 cgcttcccgg gcgcgcgatt gtcaacagcg acggcgcgga ggattaagct gcgcgggttt
      961 atcgggagtc gtcgcatgaa gtcatcgtca tcgtcgtcca cgcgagccgt cgctcgctcg
     1021 ctcgccggcg cggcgatcgc cgcactggcc atgaccgcgg cgcacgcggc atccgacgtg
     1081 cgggtgctgc tcggcgtcga tccggccgac acgaccggcg acgtgctgct cagcgcttcg
     1141 ctcggcccgg cgcagtcgct cggccgcgcc ctcggcagcc gcgtgacgat cacgcagaca
     1201 tcccggatgt tcgatgtgat gcgcgccagc cgcacggtcg agaacgacat catcatcgcc
     1261 ccggcgcacg tcaccgcgtc ggcgatcctg cacgagtacc agttgctcgc cacgagcggc
     1321 caggaccagg tctacgtgct gatggcgaag aagggcatcg attcggtcga caagctgccg
     1381 cacaagcgcc tctatctgcc gcagcaggat tcgctgcgaa gctacgtcgc caagggcctg
     1441 ctcaccgact cgggcgtcaa gctcaaccag ttctccaagg tcacttacgg caataccagc
     1501 ggagccggcc tggtcgccct gtcgttcgac atggccgacg tcaccatcac cgacgaggcc
     1561 caggccaagc agtggatcgc gacgcacccg ggcggcgcgc acatcctgaa gacgacccga
     1621 ccggtgccgg gcggaatgag catcgtcgtc cgcaaggact tctgcgtcac cgaatgcgcc
     1681 cgcatcgccg agtggatcaa ctcgccggac ggcgtcattc cgggcatcgg ccggttccgc
     1741 atcgcctcgg ccgacgccgg caagcagttc acctacgtcg cctcgctcgg catcgcgacc
     1801 cccgagggcg tcaagggcgc gacccgagtc agcgccgacc aggtcgccga cctgatgaag
     1861 cagggtgtca cgctcgtcga cacgcgcacg cagaaggaat acgacagcga gcacgtgcgt
     1921 ggcgcgatcc tcgcccccta cggcgagaag agcctgaagg agatcgactt cgacgcgtcg
     1981 aaggacgact accacgccat cgatgcgctg ccgaaggaca agccgctcat cctgatgtgc
     2041 aacggcaccg agtgctggaa gtcgtacaag gcgacgcgcc atgccgtcgt cgccggcttc
     2101 aagacgatct actggttccg cggcggcatg ccggagtggc gcgagaagcg cctgccggtc
     2161 gatggcactg ccggcgcgtc gatggcgagg acgacggcgc cggcgaagtc tggcggccgc
     2221 gccgtcgtcg ccgcgagcca ctgatccggc cgccggcagc gactcgcgtt cgccagcgcc
     2281 acgccctccg gcgagcgggg gcgcgccgat cgttgtttcc cctgccgcgg accggcgatc
     2341 gcgccgcgtc gtcgacatcg ttgccggcgg cgccagctcg tcggcgagct cgccgacccg
     2401 ccacgcggct gcgcagcgtt ctccgggccg ccggccaacg gcagcggttt gtaccaatgg
     2461 agcgaagctg accttgcggc acagtcccgc acggcgcgat cctgcggatc gcggggagca
     2521 agcggg
//
LOCUS       DASRWS010000455         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3067808, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000455 DASRWS010000000
VERSION     DASRWS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067808"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFF72_08585"
     CDS             <1..889
                     /locus_tag="VFF72_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZV93261.1"
                     /translation="TISLSAAIVHFGGMGGMGGMGMLMGMAQGYGDGHAIAGIIGGFV
                     LWALAASLVGQVYLLGLCLVYLRVTEGLDPSAAEAALRERLDEARRRTAELGDRARAA
                     TQRDGGHSAAAATTAAAASSAATMAATPPVSPGGFAEGGAVPPAYTPPPFAPPPPFPA
                     SPAPTGGAPGWVPAPPPPFTAAAPSPPYPASSAETYPPPSMPEPGPRAMPHGGAMPHD
                     PAEAADISLPFDDLPSPGGPAAVAPPVYPAPPAWSAPPAAAVVPPAPAPAPLTTCPQC
                     LSPVTSDDIFCGVCGYRLK"
     gene            912..1703
                     /locus_tag="VFF72_08590"
     CDS             912..1703
                     /locus_tag="VFF72_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93262.1"
                     /translation="MNEHDFNSARDALIDARRRPAALASYPASWQPDDLADAYRLQAA
                     VIDAFGPVGAWKVAAVTQAQQQAMRLVGPIAGAIPACHVHDAASGAARLRLADFIAPL
                     IECEFAFELARDLPERDAAYTRAEVGDAVAALRLAVEIVDPRLPRDAGTMSQIADGFN
                     NGALVAGPRIERWSSVDFAATTIVLRAVGVAESVATGSGRAILDGDPLGALVLLANVH
                     ASGRGLRRGDIVTTGSCTGAPRLPGPGLYRAEFASLGGVDLRVDG"
     gene            complement(1730..2332)
                     /locus_tag="VFF72_08595"
     CDS             complement(1730..2332)
                     /locus_tag="VFF72_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZV93263.1"
                     /translation="MQHTFVSAFILLLLVLDPFGSLPIFIPVMQAVAPRRRRAVALRE
                     VAIAFAVLCFFLFFGEGFLRVMRLSERSLEVAGGVILLMVAIRMIFNQGAGIYGLPDG
                     REPFVFPLAVPLLAGPSAMATVLLLASQQPDRLGEWSAALACAMAVSGLMLVGCARIH
                     RWLGDSVVSALEKLMGLVLTAIAVEMILAGLKRYFFLTPT"
BASE COUNT          386 a          990 c          911 g          353 t
ORIGIN      
        1 gacgatctcg ctttccgcgg cgatcgtcca tttcggcggc atgggcggca tgggcggcat
       61 gggcatgctg atgggaatgg cgcaaggcta cggcgacggg cacgccatcg ccggcatcat
      121 cggtggcttc gtcctgtggg cgcttgcggc gtcgctggtc ggccaggtct atctgctcgg
      181 cctgtgcctc gtctatcttc gcgtcaccga agggctcgac ccgagcgccg cggaagccgc
      241 cctgcgcgag cgcctcgacg aggcgagacg ccggaccgcc gagctcggcg acagggcccg
      301 cgcggcgacg cagcgcgacg gcggtcattc ggcagcggcg gcgacgacgg ccgccgccgc
      361 atcctcggcc gccaccatgg ccgcaacgcc ccctgtgtcg ccgggcggat tcgccgaggg
      421 cggtgcggtg ccgccggcct atacgccgcc ccccttcgcc cctccgccgc cattcccggc
      481 gtcgccggca ccaaccggcg gcgcgcccgg ctgggtgccg gcgccgccgc cgccgttcac
      541 ggcggcggcg ccttctccgc cctatcccgc gtcatccgcc gaaacctatc cgccgccgtc
      601 gatgcccgaa ccggggccac gtgccatgcc gcacggcggc gcgatgccgc acgaccccgc
      661 cgaagcggcc gacatttccc tgccgttcga cgacctgccg tcgcccggcg ggccggccgc
      721 cgtggcgccc ccggtctatc cggcgccccc cgcgtggtcg gcgccgccgg ctgcggcggt
      781 cgtgccgcct gcgccggccc cggcgccctt gacgacctgc ccgcaatgcc tctccccggt
      841 cacgtcggac gacatcttct gcggcgtctg cggctaccga ctcaaatgac aggaagcgcc
      901 ccgtcgcgac gatgaacgag catgatttca actcggcgcg cgacgcgctg atcgacgccc
      961 ggcgccgacc cgccgccctc gcctcctatc ctgcgtcctg gcagccggac gacctcgccg
     1021 acgcgtaccg gctgcaggcg gcggtgatcg atgcgttcgg cccggtgggc gcgtggaaag
     1081 tcgccgcagt gacgcaggcg cagcagcagg cgatgcggct cgtcggcccg atcgccggag
     1141 cgatcccggc ttgtcatgtg cacgacgccg cctccggcgc cgcccgactg aggctggccg
     1201 atttcatcgc cccgctgatc gaatgcgagt tcgccttcga gctcgcgcgc gacctgcccg
     1261 agcgtgacgc cgcatatacc cgcgccgagg tgggcgacgc ggtcgcagca cttcgcctgg
     1321 cggtcgagat cgtcgatccg cgcctgccgc gcgatgccgg aacgatgtcg cagatcgccg
     1381 atggcttcaa caacggcgcg ctcgtcgccg gacctcgcat cgagcggtgg tcatcggtcg
     1441 actttgcggc caccacgatc gtcctgcggg ccgttggcgt ggcggagtcg gtcgcaaccg
     1501 gcagcggccg agccatcctc gacggcgacc cgctgggcgc gctggttctc ctcgccaacg
     1561 tccatgcctc ggggcgcggc ttgcgacgcg gcgacatcgt cacgaccgga agctgcaccg
     1621 gcgcgccgcg gcttccaggc ccgggccttt atcgcgcgga attcgcctcg ctcggcggcg
     1681 tcgatctgcg cgtcgacggc tgagggcggg tcgggcggcg ctcgccgctt caggtcggcg
     1741 tcaggaagaa gtagcgcttc aggccggcga ggatcatctc gacggcgatc gccgtcagca
     1801 ccaaccccat caacttttcc agcgcggaaa cgacggagtc gccgagccag cggtggatgc
     1861 gggcgcagcc gacgagcatc aggcccgata ccgccatcgc gcaggccagc gccgcgctcc
     1921 actccccgag ccggtcgggt tgctgcgacg ccagcagcag caccgttgcc atcgccgagg
     1981 gaccggccag gagcggcacg gcgagcggaa agacgaacgg ctcgcgcccg tcgggcaagc
     2041 cgtagatgcc cgcgccctgg ttgaagatca ttcgaatcgc caccatcaag aggatgactc
     2101 cgccggcgac ctcgagcgac ctttcggaaa ggcgcatcac gcgcaggaac ccttcgccga
     2161 agaacaagaa gaagcagagc acggcgaacg cgatcgccac ctcgcgcaag gcgacagcgc
     2221 ggcggcgccg cggcgcaacc gcctgcatca cgggaatgaa gatcggcaag ctgccgaagg
     2281 ggtcgaggac aagcagcagg aggatgaacg ccgagacgaa ggtgtgttgc atcgtcgggc
     2341 gcgctcagtg cggcgcacgc ttcgacagcg caggcgacgc gcacgtcttg cgcaatcctc
     2401 gtgcacccgc gctgaccttt ccggtgccgc ggggcggcgc atcggcccga ccgtgcgcgc
     2461 gcccgacgat ggtcgtgtca gacatggacg acccgccccg ccagtcgata cacgaggtac
     2521 gcgaccaggg cgacgacgac gacccagcga agcagcgcac cgtcgcgccg cacctcgccc
     2581 ggcgagcggt cgccgacgcc gggcgcacga tcgcgaaagc gcagtgcgtc gtgcatttcc
//
LOCUS       DASRWS010000456         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1048470, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000456 DASRWS010000000
VERSION     DASRWS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048470"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VFF72_08600"
     CDS             <1..1887
                     /locus_tag="VFF72_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZV93264.1"
                     /translation="ERPDIDQIYMNALHLLGEQGGWPMTMFLTPSGEPVWGGTYFPKD
                     ARFGRPAFVDVLREVSRLFREEPAKIAKNRAALLASLAQKARPEGKVVIGAEELDAAA
                     RQVGNMFDQVNGGLRGAPKFPQPAILEMLWRAGLRSLSSSPGRREAPSPGDPRLSSEG
                     KDVDGRDKPGHDGGGAMYAGDARFFETVEHSLERMCEGGIYDHLGGGFSRYSVDDRWL
                     VPHFEKMLYDNAQLLDLLALAWQRGGNPLFATRARETVGWLAREMTTRDGAFCASLDA
                     DSEGEEGKFYVWSKNEIIELIGPEAAEFFAAHYDVSDEGNFEGHNILNRLASVPRSGD
                     DEARLASLRAILLDARAARVRPGLDDKVLADWNGLTIAALVNASIVFDAPEWLAMAKR
                     AFAFIAGGMTRGDRLGHSFRDGKLLLPGLASDYAAMIRAALALYEATGERGYLDGALA
                     WQRAFDAHYSDAETGAYYLSADDASDLLLRPHATADDAVPNANSVAAGNLVRLAVLAG
                     DDAFRARADRLMEHILSANARNLLGHVALLNALDLRLRGAEIVAVGADAERFAEAALR
                     LPFVDRIVLRAASAADLPATHPAQVKLHAISGSAAFVCVGERCSLPVMDAGDLAAAVA
                     AMRK"
     gene            1921..>2286
                     /locus_tag="VFF72_08605"
     CDS             1921..>2286
                     /locus_tag="VFF72_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZV93265.1"
                     /translation="MKNIEKVAVIGLGTLGSQIAMQAAAYGFDVVGFDPNAVAFATSQ
                     EKIRSAMQMVHKGPTYPVARWDEIAGKVRLVSDLATAVKNADLVIEVVAENLELKREV
                     FAELDRLAPADALLATNSSS"
BASE COUNT          356 a          762 c          787 g          381 t
ORIGIN      
        1 gagcggcccg acatcgacca gatctacatg aacgcgctgc atctgctggg cgagcagggc
       61 ggctggccga tgacgatgtt cttgacgccc tcgggcgagc cggtgtgggg cgggacgtat
      121 ttcccgaaag acgcgcgctt cggccggccg gccttcgtcg acgtgctgcg cgaggtgtcg
      181 cggttgttcc gcgaggagcc cgccaagatc gcgaagaacc gtgcggcgct gctcgccagc
      241 ctggcgcaga aggcccggcc cgagggcaag gtcgtcatcg gcgcggagga gctcgatgcc
      301 gccgcgcgcc aggtcggcaa catgttcgac caggtcaatg gcggcctgcg cggcgcgccg
      361 aaattcccgc agccggcgat tctcgaaatg ctctggcgcg cgggattgcg gtcactttcg
      421 tcatcaccgg gccggcgcga agcgccgagt cccggtgatc cacgcctttc ttccgaaggc
      481 aaagacgtgg atggccggga caagcccggc catgacggag gaggcgccat gtacgccggc
      541 gatgcgcgct tcttcgagac cgtcgagcac tccctcgagc gcatgtgcga gggcggcatc
      601 tacgatcatc tcggcggcgg cttctcgcgc tattcggtcg acgaccgctg gctggtgccg
      661 catttcgaga agatgcttta cgacaacgcg cagcttctcg acctgctggc gctcgcctgg
      721 cagcgcggcg gtaacccgct gtttgcgacg cgtgcgcgcg agaccgtcgg ctggcttgcc
      781 cgcgagatga cgaccaggga tggcgccttc tgcgcctcgc tcgatgccga ctcggaaggc
      841 gaggagggca agttctacgt ctggtcgaag aacgagatca tcgagttgat cgggccggaa
      901 gcggccgagt tcttcgccgc gcattacgat gtcagcgacg agggcaattt cgagggccac
      961 aacatcctca atcgtctcgc aagcgtgccg cgctcgggcg atgacgaggc gcggctcgca
     1021 tcgttgcgcg ccatcctgct cgatgcgcgc gcggcccgcg tgcggcccgg cctcgacgac
     1081 aaggtgctgg ccgactggaa cggcctgacg atcgcggcgc tggtcaatgc gtcgatcgtg
     1141 ttcgacgcgc cggaatggct ggcgatggcg aagcgcgcct tcgccttcat tgccggcggc
     1201 atgacgcgcg gcgaccggct cggccattcg ttccgcgacg gcaaactgct gctgccgggg
     1261 ctggcgtcgg actatgcggc gatgatccgc gccgcgctgg ctttgtacga ggcgaccggc
     1321 gagcgcggct atctcgacgg cgcgcttgcc tggcagcgcg ccttcgacgc ccattacagc
     1381 gacgccgaga ccggcgccta ttatctctcc gccgatgacg cgtccgacct gctgctgcgg
     1441 ccgcatgcga ccgccgacga cgcggtgcca aacgccaatt cggtcgccgc cggcaacctg
     1501 gtgcggctcg cggtgctcgc cggcgacgac gccttccgcg cccgcgccga ccgcctgatg
     1561 gagcacatcc tgtcggccaa tgcgcgcaat ctgttgggcc atgtcgcgct tctgaacgcg
     1621 ctcgacctgc gtctgcgcgg cgccgagatc gtcgccgtcg gcgccgacgc cgagcgtttc
     1681 gccgaggccg cgctcagact gcccttcgtc gatcgcatcg tgctgcgcgc ggcaagcgcc
     1741 gccgatcttc ccgcgacgca tccggcgcag gtgaagctcc atgcgatttc gggtagcgcc
     1801 gccttcgtgt gtgtcggcga acgctgctcg ctgccggtga tggatgcggg cgatcttgcc
     1861 gcggcggtcg cggcaatgag aaagtaatgc tcgcagaacg ctcacgtccg ggaacgcagc
     1921 atgaaaaaca ttgagaaggt cgccgtcatc gggctcggca cgctcggcag ccagatcgcg
     1981 atgcaggccg ccgcctacgg cttcgacgtc gtcggcttcg atccgaacgc ggtagcgttc
     2041 gcgacatcgc aggagaaaat ccgctcggcg atgcagatgg tgcacaaggg cccgacctat
     2101 ccggtcgcgc gctgggacga gatcgccggc aaggttcggc tggtgagcga cctcgcgact
     2161 gccgtgaaga acgccgatct cgtcatcgaa gtggtcgccg aaaacctcga actcaagcgc
     2221 gaggtgtttg ccgagctcga ccggcttgcg ccggccgacg ccctcttggc gaccaacagt
     2281 tcgtcg
//
LOCUS       DASRWS010000457         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4209979, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000457 DASRWS010000000
VERSION     DASRWS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4209979"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VFF72_08610"
     CDS             <1..173
                     /locus_tag="VFF72_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZV93266.1"
                     /translation="GILLSALYDPVWTSAIHSRTDFGLGLAAFGLLVHARLSPFIVVA
                     LCAFAGWALTSV"
     gene            complement(398..1285)
                     /locus_tag="VFF72_08615"
     CDS             complement(398..1285)
                     /locus_tag="VFF72_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZV93267.1"
                     /translation="MERDAAKPVDRAPRPDAANGSPGWLQFFGRIRKQVLDERLNVIA
                     AGVAFYGLLATFPAITAVVSIYGLLFDSRQATRQLASLSTVLPQQAAGLVVNQLTAIA
                     AAGHAALGLGVAGSVLVALWSSSAGVRALMKALNVAYGEEEGRAGWFRVGLSVFLALG
                     AIAGGLVVLAVILFLPAIGSVLNLGGPLRSAIEWIRWPVIAASFWLAIALLYRFGPDR
                     GAAHWSWKNWGALCAVVLWLAGSALFAWYVSRFGSYNRIYGSLGAVVVLLIWFLLSSW
                     SVLVGAEVNAIVEGHRRAD"
     gene            complement(1319..1786)
                     /locus_tag="VFF72_08620"
     CDS             complement(1319..1786)
                     /locus_tag="VFF72_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93268.1"
                     /translation="MSAQLRSLRGGGSAGVVFASDGVSLVAGGGEFCATIVELEVPVD
                     AIELLLEDGAGVELGMFPPCATDASGFVALALPIVSPEWAQPIAKAATAADDASNAKR
                     PENLFMIRPFVILPSVAESPRQPVCPGRPGRGDIGSREARVPSTRLIARAIGA"
     gene            complement(1783..>2172)
                     /locus_tag="VFF72_08625"
     CDS             complement(1783..>2172)
                     /locus_tag="VFF72_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93269.1"
                     /translation="IADIRPAERTTAAGRFVTVPGHDTKGHRVVWIDYAAGLSMHTVV
                     TDVPAERRLQRMASADPAEHRISYGCVNLPARFFEDVAAPAFAKGRGVIYILPDTRRL
                     EDVFPGLAGSTVARRPAAALLARATDR"
BASE COUNT          360 a          751 c          746 g          315 t
ORIGIN      
        1 tcggcatcct gttgtcggcg ttgtacgacc cggtctggac gagcgccatc cattcgcgca
       61 ccgacttcgg tctcgggctg gcggcgttcg ggcttctcgt ccatgcccga ctgtcgccct
      121 tcatcgtggt cgccctctgc gccttcgccg gctgggccct gacaagcgtc tgaaccgccg
      181 cgctcgaacg gcaatgcgcg ggcgcgggtg cgggcgtgtt cgtgttcgtc tgcgtgtgcg
      241 tgtgcgtctg cgtctgaatg ccctcgggtc gcttctccgc gggcacgcgt cggatcgccc
      301 tgctcggtcg gcacgtcgat ccctggtgcc ttcttgggca tcgacgacgc tgactgcaac
      361 gacggccccg cgacacgcgc gcaggcgcgc cagcttttca gtccgcccga cggtggcctt
      421 cgacgatggc gttgacctcg gcgccgacga gaaccgacca cgacgacagc aggaaccaga
      481 tcaggagaac gacgaccgct ccgagcgagc cgtagatgcg gttgtagctg ccgaagcggc
      541 tgacgtacca ggcaaagagc gccgagccgg cgagccagag aacgaccgcg caaagcgcgc
      601 cccagttctt ccacgaccaa tgcgccgcac cccgatcggg gccgaagcga tagaggagcg
      661 cgatggcgag ccagaaggaa gcggcgatga cgggccagcg gatccactcg atggccgagc
      721 gcaacggccc gccgaggttg aggacgctgc cgatcgccgg caggaagagg atgactgcca
      781 ggacgacgag accgccggcg atcgcgccca gcgccaggaa cacggaaagg cccacccgga
      841 accagccggc gcgcccttcc tcctcgccgt acgcgacgtt cagggctttc atgagcgcgc
      901 gaacgcctgc cgagctgctc cacaaggcca ccaggacgct gccggcgacg ccaaggccca
      961 gcgcggcgtg ccccgccgcg gcgatcgcgg tcagctggtt cacgacgagg ccggccgcct
     1021 gctgcggaag gacggtgctg agcgatgcga gctggcgcgt cgcctgccgg gaatcgaaca
     1081 gcagtccgta gatcgacacg acggccgtga tggcgggaaa ggtcgcgagc aggccgtaga
     1141 acgccacgcc cgccgcgatc acgttcagcc gctcgtcgag aacctgtttc ctgatgcggc
     1201 cgaagaactg cagccagccc ggcgacccgt tcgccgcgtc gggccgaggc gcccgatcga
     1261 caggcttcgc tgcatcgcgt tccatgacga ctcctgtgcc tcgatgaatc ttctgcgctc
     1321 aggcgccgat cgcccgggcg atgagccgtg tcgatggcac cctcgcctcg cgcgagccga
     1381 tgtcgccacg ccccggccgt cccgggcata ccggttgcct cggactctcc gccacacttg
     1441 gcaggatgac gaaagggcga atcatgaaca ggttctctgg tcgcttggcg ttgcttgcat
     1501 cgtcggcggc agttgccgcc ttggcgatgg gctgcgccca ctcaggcgac acgatgggca
     1561 gcgccagcgc gacgaatccg gatgcatcgg tcgcgcaagg cggaaacatg ccgagctcga
     1621 cgccggcgcc gtcttcgagc agcaattcga tggcgtcgac gggtacctcg agctcgacga
     1681 tggtcgcgca gaactcgccg ccgccggcga ccagcgagac gccgtcggac gcgaacacga
     1741 ccccggccga accgccgccg cgcagcgacc gcaactgagc gctcaacggt cggtggcgcg
     1801 cgcgagcaac gctgccgccg gccttcgagc cacggtgctc ccggccagac cgggaaagac
     1861 gtcctcgagt cgacgcgtgt cgggaaggat gtagatcaca ccgcgtcctt tcgcgaaagc
     1921 gggcgccgcg acgtcctcga aaaatctcgc gggcaggttg acgcagccgt acgagatgcg
     1981 gtgctcggcc ggatcggccg aggccatgcg ctggagccgt cgttcggcgg gaacatcggt
     2041 gacgacggtg tgcatcgaca ggcccgcggc atagtcgatc cagacgacgc ggtgaccctt
     2101 ggtgtcgtgt ccgggcaccg tcacgaagcg gccggccgcc gtcgttcgct cggccgggcg
     2161 gatatcggcg at
//
LOCUS       DASRWS010000458         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_776571, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000458 DASRWS010000000
VERSION     DASRWS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776571"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFF72_08630"
     CDS             complement(<1..457)
                     /locus_tag="VFF72_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HZV93270.1"
                     /translation="MAVADVFDAAQPPAMTPRLIDAFWRAVAYCLHPKVVALSLAPLA
                     IVGGSAALLGYLGWEPAVGAVRAWLEDWSLLAAFFGWLDAIGAAGLHSVVAPLVVVAL
                     AVPIFVVASLVVVAWLMTPAIVRLVATRRFPDLERRRGAGFWQSIGWSLV"
     gene            723..2009
                     /locus_tag="VFF72_08635"
     CDS             723..2009
                     /locus_tag="VFF72_08635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZV93271.1"
                     /translation="MLAHPATLLAAASASLFISSAAAATIGVRQKLRRGVWWWIGANL
                     GLAAALVLHALGDDNPLFAPLAAVCALQWPIVTLTGMRRFFSRGGGALPAWADWLAIG
                     LASMFAAGVAIVPIGWVGPAQVLAVAMLAVTLYVALAIARLEDFETTPILKSLLVCLV
                     CSAGLQAVWLGMAVFVLAIGGADVALGALLAPAATALLMPQLSLVMNHERNVAQLRAS
                     HRKLRHMVEVDALTKLPNRRHFHDLAAKAIAPAPDLATMLVFDVDRLKNINEVLGHSV
                     GDEALRQIGMALRETLRRRDVAGRLGGDEFAVVLPRTRVTDIDAVVSRINARIDDRQV
                     APRIARVCLNAGATQIQAGESIADALRRAEITLETMRDKRRAAEELAGRMADSMPAEQ
                     AIPALEALATHRGPVSAAAPLNLIPVGEVFAGSNAG"
     gene            complement(2060..>3272)
                     /locus_tag="VFF72_08640"
     CDS             complement(2060..>3272)
                     /locus_tag="VFF72_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZV93272.1"
                     /translation="FRSGVVAGREALARTLTAEVGKPISQSRREIDGFLGRIDFFLAE
                     VQPALAREDVFVDASLTERIGHEPLGVIANISAWNYPFFVGGNVFVPALLTGNAVLYK
                     PSELATLTGLNIGRLLHAAGVPREVFAVVVGAAQTGEALLAQRLDGVFFTGSAATGRA
                     IAATIGPRMIRMQLELGGKDPSYVADDADPLAAAASLADGAMYNAGQSCCSVERIYVH
                     DRIHDAFVDAFVAVVAGLKVGDPDDEATDVGAIARAAQLDVLDAQVADAVARGGRLCT
                     GGRRIRRAGNWFEPTVVTAANHDMLLMRDESFGPVIGICKVAGDAEAVALMNDTRYGL
                     TAGVYTPDEARATRLLAQLRAGSVYWNCCDRVSPRLPWSGVGDSGIGLTLSRRGIETF
                     TRPKAWHLRRP"
BASE COUNT          498 a         1200 c         1130 g          444 t
ORIGIN      
        1 agaccagcga ccagccgatc gactgccaga agccggctcc gcgacgccgc tccaggtcgg
       61 ggaatcgtcg cgtcgccacc agccggacga tcgcgggcgt catcagccag gcgacgacga
      121 ccagcgatgc gacgacgaag atcggtaccg ccagggcaac gacgaccagc ggcgcgacga
      181 cgctgtgcag gccggcggcg ccgatcgcgt cgagccagcc gaagaacgcc gccagcaacg
      241 accagtcctc gagccacgcg cgcaccgcgc cgaccgccgg ctcccagccg agatagccga
      301 gcagggcggc gctgccgccg acgatcgcca gcggcgcgag cgacagcgcg acgaccttcg
      361 gatggaggca ataggcgacg gcgcgccaga acgcgtcgat caggcgagga gtcatggcgg
      421 gcggctgcgc cgcgtcgaag acgtcggcaa ccgccattgt gtgaccttcg accaacccgt
      481 cgattgccgc tggccaacgg tgtgccgcca ggtgtcgcaa gtcgatcccc cacctccccg
      541 aaaggcgtgc gtagtcgacg ccactcgtct gggcaaacca gaccgcgggt gatcgccccg
      601 tgcggcgcgc cttccggtcg ccgacgccag gcgtgccaat tcgtaacgga attggcgacg
      661 ccggggcgcc gcgccgacgc tgcacgcccc tgcgccggaa gtcgctgcct agaatttcgc
      721 caatgctcgc gcaccccgcc accttgctcg ccgcggcctc ggccagcctc ttcatctcga
      781 gcgctgccgc agccaccatc ggcgtacgcc agaagctgcg ccgcggcgtc tggtggtgga
      841 tcggcgccaa tctcggcctc gcggcagcgc tcgtgctgca cgccctgggc gacgacaacc
      901 cgttgttcgc accgctcgcc gccgtctgcg ccctgcagtg gccgatcgtg acgttgacgg
      961 gcatgcgtcg gttcttctcg cgcggcggcg gcgcccttcc cgcctgggcc gactggctgg
     1021 caatcggcct ggcgtcgatg ttcgctgccg gcgtggcgat cgtgccgatc ggctgggtcg
     1081 ggccggccca ggtgctcgcc gtcgccatgc tcgcggtgac gctctacgtg gcgctggcga
     1141 tcgcgcggct ggaggacttc gagacgacgc cgatcctgaa gagcctgctc gtctgcctcg
     1201 tctgttcagc cgggctgcag gcggtgtggc tcggcatggc agtgttcgtc ctggcgatcg
     1261 gcggcgccga cgtggcgctc ggcgccctgc tcgcgcccgc ggcgacggcg ctcctgatgc
     1321 cgcagctctc gctggtcatg aaccacgagc gcaacgtcgc ccagctgcgc gcgtcgcacc
     1381 gcaagctgcg ccacatggtc gaggtcgacg cgctgaccaa gctgcccaat cgacgtcatt
     1441 tccacgacct cgccgccaag gccatcgccc cggcgcccga cctggcgacg atgctcgtct
     1501 tcgacgtcga caggctgaag aacatcaacg aggtgctcgg ccattcggtc ggcgacgaag
     1561 cgctgcgcca gatcggcatg gcgctgcgcg agacgctgcg ccgccgcgac gtcgccgggc
     1621 gcctcggcgg cgacgagttc gcggtcgtcc tgccgcgcac ccgggtcacc gacatcgacg
     1681 ccgtcgtcag tcgcatcaac gcgcgaatcg acgaccgcca agtggcgccc cggatcgcgc
     1741 gcgtctgcct caacgccggt gccacccaga tccaggccgg agaatcgatc gccgacgcat
     1801 tgcgacgcgc cgagatcacg ctcgagacga tgcgcgacaa gcggcgcgcc gccgaagagc
     1861 tcgccggccg gatggccgac tcgatgccgg ccgagcaggc gatcccggcc ctcgaggcgc
     1921 tggccacgca tcgcggcccg gtctcggccg cggcgccgct caacctcatt ccggtcggcg
     1981 aagtcttcgc aggatcgaac gcgggctgag ggaacccggc gccggcaggt ctctcgacgg
     2041 cgctggttct cccgacggct cagggtcgtc gaaggtgcca ggccttcggc cgcgtgaacg
     2101 tctcgatgcc cctgcgcgac agcgtcaggc cgatgcccga atcgccgacg cccgaccaag
     2161 gcaggcgcgg gctgacccgg tcgcagcagt tccagtagac gctgccggca cgcagctgcg
     2221 cgagcaggcg cgtcgcccgc gcctcgtccg gtgtgtagac gccagcggtg agcccgtagc
     2281 gcgtgtcgtt catcagcgcc accgcctcgg catcgcccgc caccttgcag atgccgatga
     2341 ccggtccgaa actctcgtcg cgcatcagca gcatgtcgtg gttcgcggcc gtgacgacag
     2401 tcggctcgaa ccagttgccg gctcgccgga tgcgccggcc gccggtgcac agacggccgc
     2461 ctcgggcgac tgcatcggcc acctgcgcat cgagcacgtc gagctgggcc gctctcgcga
     2521 tggcgccgac gtccgtcgcc tcgtcgtccg gatcgccgac cttcaggccg gcgacgacag
     2581 cgacgaaagc gtcgacgaag gcgtcgtgga tgcgatcgtg gacgtagatg cgctcgaccg
     2641 agcagcagct ctggccggcg ttgtacatcg cgccgtcggc cagcgacgcc gccgcggcga
     2701 gcggatcggc atcgtcggcg acgtagctcg ggtccttgcc gccgagctcg agctgcatgc
     2761 ggatcatgcg cgggccgatc gtcgcagcga tcgccctccc cgtcgcggcc gacccggtga
     2821 agaagacgcc gtcgagccgc tgcgccagga gcgcctcgcc cgtctgcgcc gcgccgacga
     2881 cgacagcgaa aacctcgcgc ggcacgcccg ccgcgtgcag gaggcggccg atgttcaggc
     2941 cggtcagcgt cgccagctcc gacggcttgt agagaacggc gttgccggtc agcagcgccg
     3001 ggacaaagac gttgccgccg acgaagaacg ggtagttcca ggccgagatg ttggcgatga
     3061 cgccgagcgg ctcatggccg atccgctcgg tgagcgaagc gtcgacgaag acgtcctcgc
     3121 gcgccaacgc cggctgcacc tcggcgagaa agaagtcgat gcggccgagg aacccatcga
     3181 tctcgcggcg cgactgcgag atcggcttgc cgacctcggc cgtgagcgtg cgggccagcg
     3241 cctcgcgccc ggcgacgacg cctgagcgaa ag
//
LOCUS       DASRWS010000459         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_776693, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000459 DASRWS010000000
VERSION     DASRWS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776693"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..801
                     /locus_tag="VFF72_08645"
     CDS             64..801
                     /locus_tag="VFF72_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZV93273.1"
                     /translation="MSRFAAVARSAIFFVFLAVTVVPWATVAVAYSIFVRGERLYWVC
                     AGWLGVAVWGARVICGVQARVQGRENLPTTSVILLSKHQSAWETLAYPTLMPRPLCYV
                     FKRELLWIPFFGWAMARMDLIHIDRSKRSEAWNKVAEQGRRFMAQGNWVIMFPEGTRT
                     PRGGQGVYKQGGTRLAVETGRPVLPVAVTSARCWPRKSLVLSPGVVDISIGPPIDSSG
                     HDSASLMREVERWIETEMRRLDPEAYR"
     gene            798..1790
                     /locus_tag="VFF72_08650"
     CDS             798..1790
                     /locus_tag="VFF72_08650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZV93274.1"
                     /translation="MNARVGRALRRVVDVLQRGLFDDPDPDPELPPQAETPTVAPTPG
                     KGPSRVDDVHRHPRANREAVLDGRRVAFLLRRSRRRSIGFVVGAEGLVVSAPKWVTLR
                     DIDAAVREKSRWILARLAEQAARTMRVEASRIVWRDGAELPYLGETICIVLDARRGLA
                     AGEVVLDEAVPRHGAESPSEATANDRVAAPERGCRPGASPRRRLHVGLPADAGADRLR
                     DAVQSWLQREARAVFEARCSHFAGRLGVRVTRLSLSSAATRWGSASASGAVRLHWRLI
                     HYPLATIDYVVAHELAHLREMNHSPRFWNVVRSVLPDYEQARRELHGERLTAFE"
     gene            complement(1850..>2249)
                     /locus_tag="VFF72_08655"
     CDS             complement(1850..>2249)
                     /locus_tag="VFF72_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV93275.1"
                     /translation="VLPRISTNVSVTDIEPESDPLTLYIDSIERMRSIPADSLVLPSH
                     GRPFRGLHTRIDQLQSHHDARFVDVMEALATKGPQSAFELVPVIFRRPLDLHQMTFAM
                     GESIAHLHALWYRGRLERRLEGDVFRFAPV"
BASE COUNT          316 a          764 c          812 g          357 t
ORIGIN      
        1 ccgattcgct gttcggggag ctcggcggat gagccgccgg ttcgccccac cgagcgcagc
       61 cgaatgagcc gcttcgccgc ggtcgcgcgt tccgcgatct tcttcgtctt tctcgcggtc
      121 acggtcgtgc cctgggcgac cgtcgccgtc gcctattcga tcttcgttcg cggcgagcgt
      181 ctctactggg tgtgcgcggg ctggctcggt gtcgcggtgt ggggcgcacg ggtcatttgc
      241 ggcgtccagg cccgcgtgca gggtcgcgag aacctgccga cgacctcggt catcttgctg
      301 tccaagcatc agtcggcctg ggaaacgctg gcctatccga cgctcatgcc caggccgctg
      361 tgctacgtgt tcaagcgcga gctgctctgg atcccgttct tcggctgggc gatggcgcgc
      421 atggacctga tccacatcga tcgcagcaag cgctcggagg catggaacaa ggtcgccgag
      481 cagggccggc gcttcatggc gcagggcaac tgggtcatca tgtttccgga aggcacgcgc
      541 acgccgcgcg gcggccaggg cgtctacaag cagggcggca cgcgtctcgc cgtcgagacc
      601 ggcaggccgg tgctgccggt ggcggtgacg tcggcacgtt gctggccgcg caaaagcctg
      661 gtgctgagcc cgggcgtcgt cgacatctcg atcgggccgc ccatcgactc gtcggggcac
      721 gactcggcgt cgctgatgcg ggaggtcgag cgatggatcg agaccgagat gcggcgtctc
      781 gatccggagg cctatcgatg aacgcccggg tcggccgcgc cctgcgtcgt gtcgtcgacg
      841 tgttgcagcg aggcctgttc gacgaccccg atcccgatcc cgaactgccg ccgcaggcgg
      901 agacgccgac cgtcgcaccg acaccgggga agggaccgtc gcgggtcgac gatgtccatc
      961 gccatccccg cgccaaccgc gaagccgtcc tcgacggccg tcgggtcgcc ttcctgctgc
     1021 gccggtcgcg ccgacgcagc atcggcttcg tcgtcggcgc cgaaggcctg gtcgtcagcg
     1081 cgccgaagtg ggtgaccctg cgcgacatcg atgccgcggt gcgcgagaag agccgctgga
     1141 tcctcgccag gctggccgag caggcggctc ggacgatgcg ggtcgaggcg tcgcgcatcg
     1201 tctggcgcga cggggccgag ctgccttacc tcggcgagac gatctgcatc gtcctcgacg
     1261 cacggcgtgg cctggctgcc ggcgaagtgg tgctcgacga ggccgtgccg cgacatggtg
     1321 ccgaatcgcc aagcgaagcg acggcgaacg accgcgtcgc cgctcccgaa cggggatgcc
     1381 ggccaggcgc atcgccccgg cgtcggctgc atgtcggctt gccggccgat gcgggcgccg
     1441 accgcctgcg cgacgccgtg cagagctggc tgcagcgcga ggcgcgcgcc gtcttcgagg
     1501 cgcgatgcag tcacttcgcc gggcgcctcg gcgttcgcgt cacccggttg tcgctgtcgt
     1561 cggccgcgac gcgctggggc agcgccagcg ccagcggcgc ggttcgcctg cactggcggc
     1621 tgatccacta tccgctggcg acgatcgact acgtcgtcgc ccacgagctc gcgcatctgc
     1681 gcgagatgaa ccacagcccg cgcttctgga acgtcgtgcg ctcggtgctg cccgactacg
     1741 agcaggcgcg acgcgagctg cacggcgaga gactgacggc attcgagtga gcggctcgcg
     1801 ccgtccggcg gcggggcggg gcggctcgac ggcttcttgc cggacgaagc tagaccggcg
     1861 cgaaccggaa gacgtcgcct tcgagccgtc gctcgagccg gccgcgatac cagagcgcat
     1921 gcaggtgcgc gatcgactcg cccatcgcga aggtcatctg gtgcaggtcg agcgggcgcc
     1981 tgaagatcac cggcaccagc tcgaacgcgc tttgcgggcc tttcgtcgcc agcgcttcca
     2041 tcacatcgac gaagcgggcg tcgtgatgcg attgcagctg gtcgatgcgc gtgtgcaagc
     2101 cccgaaacgg ccgcccatgc gacggcagga ccagcgagtc cgccgggatg ctgcgcatcc
     2161 gctcgatcga atcgatgtag agcgtcagcg ggtcggattc gggttcgatg tcggtgacac
     2221 tgacgttggt cgagatgcgc ggcagcacc
//
LOCUS       DASRWS010000460         5137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1636153, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000460 DASRWS010000000
VERSION     DASRWS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636153"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFF72_08660"
     CDS             <1..329
                     /locus_tag="VFF72_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93276.1"
                     /translation="LIARFLCTGPRSFSTGQFIIWMQRAVAQVTAAGSTLDPLLQRAP
                     PEALARQRLRWLQRKKLVTGDDTLWQNAWPSDAGPGWHSPAAIVRYLANSLDDILSLA
                     VAERGP"
     gene            326..1327
                     /locus_tag="VFF72_08665"
     CDS             326..1327
                     /locus_tag="VFF72_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93277.1"
                     /translation="MNPRSVWRALARPRVFLTLLLAAALLSFAFSLGNVNRVLARMRE
                     ISGPSMLCVLGLAALYLVIKGSQLHRLLLRLRARMSWRRFVLALAVGELAATLPLGIF
                     AQNWVLLQQRSMRFGQSSSATAFMLAMEFVVAVAWLAMRGIPGWDWLRPTAALLLAAV
                     ALMLLGLPRLEPLARRLASGSAHRTVQRILVEAAGLFHGLTMLCHWRALALNFVLSSA
                     YLGTMVFAFVVVGHAMGVGTLRYFQAVTIYGFALAVLLMGGGVVGQIGSMELLGMNAA
                     RAWNIGYADGLALMLGFRLVWMGAIWLLASPLVWSLRRLIEPDPAATPAPDDVDIVG"
     gene            complement(1341..2420)
                     /locus_tag="VFF72_08670"
     CDS             complement(1341..2420)
                     /locus_tag="VFF72_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV93278.1"
                     /translation="MAGRVLFAVHDWGLGHATRSLLLIRALLARGDDVTILMAAGAGM
                     TLLRSELGDACEFYPSRDIPKPFSRYPALFYLRMSLYMPWVLIHFRLEHLLTERLVAE
                     RRFDRVVSDSRFGIWSRAVPSYCLLHSLRQIIPGRPRWLEREVERGQRQLLAGFAKVL
                     IPDVAENGGLSGDLGHDPALDWGPGRLEYIGPLAGLRSTSDVEDVDFFFSISGIEPQL
                     TLLSRLVLDALPRLRGRIVVTLGRPDQAGTQSRVGGATVHGYLDRRTQAEMVSRARVT
                     MTRSGYTTLMELAALGKRALFVPTPGQSEQEYLADLHCKRGNVWSTTQRSLDLATDLA
                     RAAAAPGLPRIACEGSAERFLAAIA"
     gene            2555..3664
                     /locus_tag="VFF72_08675"
     CDS             2555..3664
                     /locus_tag="VFF72_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HZV93279.1"
                     /translation="MSATRSPATPGSDSLAYQEQILPWVSRTFALTIPQLPPELRWVV
                     ANLYLLCRIADTIEDESALSVAQKEHFHERLVDAVSRDGDATAFAAELAPLLVTDATK
                     SEKDLVGRSADVIALTRTSGGAQRAALSRCLIVMCKGMARFERNAGLAGLRDLAAFDD
                     YCYCVAGVVGEALTALFCDYSREIAGHREEMLELSISFGQGLQMVNILKDFWDDHDRG
                     VCWLPQSLFRDGASALTALNRRMAAPDLVQAYATLIPIAHAHLRRSLRYVLLIPARET
                     GIRRFCLLALMLAMQTLRLIQRHPGFTRREQVKVSHRAVANTLIMVRLFAGSDTRLND
                     WFDGLSEDLPRQPAGLDLSPPWPGRIRSDARPALR"
     gene            3682..>5137
                     /gene="shc"
                     /locus_tag="VFF72_08680"
     CDS             3682..>5137
                     /gene="shc"
                     /locus_tag="VFF72_08680"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZV93280.1"
                     /translation="MYHTTASASARAAPPAAAPTVEVRTSREGLDAAIRAARRDVLSL
                     QHADGYWCHELEADCTIPAEYILMLHFLGESEPALEAKMARYLRRRQGRDGGWPLYEG
                     GEADVSCSVKAYFALKLAGDTAEAPHMKRARAAILALGGAARANVFTRITLALFGELP
                     WRGVPCVPIELILLPRWFWFHIDKVSYWSRTVMTPLALLCSLKARAANPLDIHIAELF
                     TTPPHLEKHYFAAPRSRLGRALLLIDRIGHRIDPRLFARLRRRAIARAESWILERLNG
                     SGGLGAIFPAMVNACEALAVLGYPPEHRLRRDARRALRELVVVNRAEAYCQPCTSPVW
                     DTALTCLALQEDLGDAPAPAIDKALSWLRERQLLQAAGDWCRAHPGLAGGGWAFQFRN
                     DAYPDLDDTAAVALAMRQADAGTRYEESLRRAADWLKGMQSRCGGFAAFDADNDRSYL
                     NEIPFADHGALLDPPTSDVSARVLTFLARLDRPGD"
BASE COUNT          755 a         1806 c         1731 g          845 t
ORIGIN      
        1 acctgatcgc ccgcttcctc tgcaccgggc cacgatcctt cagcaccggg cagttcatca
       61 tctggatgca gcgtgccgtg gcgcaggtga ccgctgccgg ctcgacgctc gacccgctgc
      121 tgcagcgcgc cccgcccgag gcgctggcgc gccagcgcct gcgctggttg cagcggaaaa
      181 agctcgtgac gggcgacgac acgctctggc aaaacgcctg gccgagcgac gccggcccgg
      241 gttggcacag cccggccgcc atcgttcgct acctcgccaa ttcgctcgac gacatcctct
      301 cgcttgcggt ggccgagcgt gggccatgaa tcctcgttcg gtctggcgcg cgctggcacg
      361 gcccagggtc ttcctgaccc ttctcctggc cgcggcgctc ctctccttcg ccttcagcct
      421 cggcaacgtg aaccgcgtcc tggcgcggat gcgggagatc tccggtccca gcatgctttg
      481 cgtgctcggc ctggccgcgc tctacctcgt catcaaggga tcgcagctgc accggctgct
      541 tctgcggctt cgggcccgca tgagctggcg gcgcttcgtc ctggcgctcg cggtcggcga
      601 actcgcggcg acgctgccgc tgggaatctt cgcccagaac tgggtgctgc tgcagcaacg
      661 ctcgatgcgc ttcgggcaga gctcgtcggc gacggcattc atgctggcga tggagttcgt
      721 ggttgccgtg gcctggctgg cgatgcgcgg catacccggc tgggactggc tgcgcccgac
      781 ggcggcgctg ttgctggcgg cggtcgcact gatgctgctc ggcctgccgc ggctcgagcc
      841 gctggcccgt cgcctggcaa gcggctcggc gcacaggacc gtgcagcgga tcctggtcga
      901 ggcagccgga ctgttccatg gcctgacgat gctgtgccac tggcgagcgc tggcgctcaa
      961 cttcgtgctc tcgtccgcgt atctcgggac catggtcttc gcgtttgtcg tcgtcggcca
     1021 cgcgatgggc gtcgggacgc tgcgctattt ccaggccgtg accatctacg gtttcgccct
     1081 ggccgtgctc ctgatgggtg gcggcgtcgt cggccagatc ggcagcatgg aattgctcgg
     1141 catgaacgcc gccagggcct ggaacatcgg ctatgccgac gggctggcgc tgatgctcgg
     1201 attccggctg gtctggatgg gcgccatctg gctgctcgcc agcccgctcg tgtggtcgct
     1261 gcgccgcctg atcgagcccg atcccgcagc cacgccagcg cccgacgacg tcgacatcgt
     1321 cggctgaatt ggcgacccct tcaggcgatg gccgcgagaa agcgctcggc cgaaccctcg
     1381 caggcgatgc ggggcagccc gggcgcggcc gcggcgcgag cgagatcggt cgcaaggtcg
     1441 aggctgcgct gcgtggtcga ccagacgttg ccgcgcttgc aatgcagatc ggcaagatat
     1501 tcctgctcgc tctgcccggg agtcggcacg aacaacgcgc gcttgccgag ggccgccaac
     1561 tccatcaacg tcgtatagcc cgagcgggtc atcgtcaccc gtgcccggct gaccatttcc
     1621 gcctgcgtcc ggcggtcgag atagccgtgg acggtcgccc cgccgacgcg gctctgcgtg
     1681 ccggcctggt cgggacggcc gagcgtgacc acgatccgcc cccgcaggcg tggcaaggca
     1741 tcgaggacga gtctcgacag cagcgtcagc tgcggctcga tgcccgagat cgagaagaag
     1801 aagtcgacgt cttcgacgtc gctcgtgctg cgaaggccgg cgagcgggcc gatgtattcg
     1861 agacggcccg ggccccagtc gagcgccggg tcgtggccca gatcgcccga caggccgccg
     1921 ttttccgcga cgtcggggat cagcaccttg gcaaagcctg cgagcaattg gcgctggccg
     1981 cgctcgacct cgcgctcgag ccatcgaggc cgcccgggaa tgatctgccg aagcgagtgc
     2041 aggaggcagt agctcggcac cgcccgcgac cagatgccga agcggctgtc ggagacgacc
     2101 cggtcgaatc ggcgctcggc gacgagccgt tcggtcagga gatgctcgag gcggaagtga
     2161 atcaacaccc agggcatgta caggctcatg cgcagataga acagcgccgg atagcggctg
     2221 aacggcttgg ggatgtcgcg cgagggatag aactcgcagg cgtcgccgag ctcgctccgc
     2281 aggagcgtca tgccggcgcc cgcagccatc aggatggtga cgtcgtcgcc gcgcgcgagc
     2341 agcgcccgga tcagcaacag gctgcgcgtc gcgtggccca gcccccagtc gtgcaccgcg
     2401 aagagaacgc gtcctgccat ggccgttgca agcggcaagc gtggtgcccg cggcgcctcc
     2461 agcaacacgg caggcgtcga caggtcggca tcccgattgc ctctgaaatt cgtcgcccgt
     2521 gtgcctgcga acaagacgaa caggagccag gaaaatgtca gcgacccggt cacccgccac
     2581 ccccgggagc gactcgctcg cctatcagga acagatcctt ccctgggttt ccaggacctt
     2641 cgccctgacg attccgcaat tgccgcccga gctgcgctgg gtggtggcca atctctacct
     2701 gctgtgcagg atcgccgaca cgatcgagga cgaatcagcg ctctcggtgg cgcaaaagga
     2761 gcactttcac gaacggctcg tcgatgccgt ttcgcgcgac ggcgacgcga cggccttcgc
     2821 ggccgagctg gcgccgctgc tggtgaccga cgccaccaaa tcggagaagg acctggtcgg
     2881 ccgcagcgcc gacgtgatcg cgctgacgag aacatcgggc ggcgcccagc gtgcggctct
     2941 atcgcgctgc ctgatcgtga tgtgcaaagg catggcgcgc ttcgagcgca acgccggact
     3001 cgccgggctg cgcgatctcg ccgccttcga cgactactgc tattgcgttg ccggcgtcgt
     3061 cggcgaggcg ctgacggcct tgttctgcga ctactcgcgc gagatcgcgg gccaccgcga
     3121 agagatgctc gagttgagca tctcgttcgg ccagggcctg cagatggtca acatcctcaa
     3181 ggacttctgg gacgaccacg accgtggcgt ctgctggttg ccgcaaagcc tgttccgcga
     3241 cggcgcctcc gcactcacgg cactcaaccg acggatggcc gcccccgacc tcgtccaggc
     3301 gtacgcgacg ctgattccga tcgcccacgc ccatctgcgc cgcagcctgc gctacgtcct
     3361 cctgattccc gcccgcgaga ccgggatccg gcgcttctgc ctgctggcgc tgatgctggc
     3421 gatgcagacc ttgcgcctga tccagcgcca tccgggcttc acgcggcgcg agcaggtgaa
     3481 ggtgtcgcat cgggccgtcg ccaacacatt gatcatggtc cggcttttcg ccggctccga
     3541 cacgcgcctg aacgactggt tcgacgggct gtcggaggat ctgccgcgac agccggcagg
     3601 gctcgacctg tcgcccccat ggccgggccg gatccgctcc gatgcccgtc ctgcgctgcg
     3661 ctgatgcgag gcgagccggc catgtatcac acgaccgcat cggcctccgc gcgggcggcg
     3721 ccaccggcag cggcgccgac cgtcgaagtg cggacgtcgc gcgaagggct cgacgccgcc
     3781 atccgtgccg cccggcgcga tgtcctgtcg ctgcagcatg ccgacgggta ttggtgccac
     3841 gagctggagg ccgattgcac gatcccggcc gaatacatcc tgatgcttca tttcctcggc
     3901 gagagcgagc cggcgctcga ggcgaagatg gcacgctatc tgcgtcgtcg ccagggccgc
     3961 gacggcggct ggccgctcta cgaaggcggc gaagccgacg tcagttgcag cgtcaaggcc
     4021 tacttcgcgt tgaagctcgc cggcgacacg gccgaggccc cgcacatgaa gcgcgcgcgc
     4081 gccgccatcc tggcgctggg cggcgccgcc cgcgccaatg tcttcacgcg gatcaccctg
     4141 gcgctgttcg gcgagctgcc atggcgcggc gtgccctgcg taccgatcga actgatcctg
     4201 ctgccgaggt ggttctggtt tcacatcgac aaggtgtcgt actggtcgcg aaccgtcatg
     4261 acgccgcttg ccttgctttg cagtttgaaa gcacgcgcgg ccaacccgct cgacatccac
     4321 atcgccgagc tcttcacgac gccgccgcat ctcgagaagc actatttcgc ggcgcctcgc
     4381 tcgaggctcg gccgggcctt gctgctgatc gaccggatcg gccaccgcat cgatccgcgc
     4441 ctgttcgcac ggctgcgtcg ccgcgccatc gcccgtgccg agtcatggat tctcgagcgc
     4501 ctgaacggca gcggcggact cggcgcgatc tttcccgcca tggtcaacgc ctgcgaagcc
     4561 ctggcggtgc tcggctatcc gcccgagcac cgcctgcgcc gcgatgcccg ccgcgcgctg
     4621 cgcgagctgg tggtggtcaa ccgcgccgaa gcctattgcc agccctgcac ttccccggtg
     4681 tgggacaccg ccctcacctg cctggcgctg caggaagacc tgggcgacgc gccggcgccg
     4741 gcgatcgaca aggcgttgag ctggctgcgc gagcgccagt tgcttcaggc cgccggcgac
     4801 tggtgccgcg cccatccggg tctcgcaggg ggcggctggg cgttccagtt tcgaaacgac
     4861 gcctacccgg atctcgacga cacggcggca gtcgccctgg cgatgcggca ggccgacgcc
     4921 ggcacgcgat acgaggagtc gctgcgccgg gccgccgact ggctgaaggg catgcagtct
     4981 cgatgcggcg gctttgccgc gttcgacgcc gacaatgatc gctcctatct caacgagatc
     5041 cctttcgccg accacggggc gttgctcgat ccgccgacca gcgacgtctc cgcgcgggta
     5101 ctgacattcc tggcccggct cgatcggccc ggcgatg
//
LOCUS       DASRWS010000461         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1050066, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000461 DASRWS010000000
VERSION     DASRWS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050066"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VFF72_08685"
     CDS             <1..128
                     /locus_tag="VFF72_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZV93281.1"
                     /translation="GGSVKDDNAASLFAESDIDGGLIGGASLNAASFVAICRAAA"
     gene            162..617
                     /gene="secG"
                     /locus_tag="VFF72_08690"
     CDS             162..617
                     /gene="secG"
                     /locus_tag="VFF72_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZV93282.1"
                     /translation="MNTVMNLVLALQILTALVMIGLVLIQHGKGADMGASFGSGASGS
                     LFGASGSANFLSRSTAVCAAVFFVCTLALAYASVDRPRASSGSVLDRPAIGAAAAASS
                     ASAVGAIPGAKPLAVPASGSAPALVPVVPAAGASTAASAAASGAAIPQK"
     gene            693..777
                     /locus_tag="VFF72_08695"
     tRNA            693..777
                     /locus_tag="VFF72_08695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:727..729,aa:Leu,seq:gag)
     gene            782..1141
                     /locus_tag="VFF72_08700"
     CDS             782..1141
                     /locus_tag="VFF72_08700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZV93283.1"
                     /translation="MILDPYLPVILFILIGVAVGVVPQILGFLLGPRKPDQAKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVSLKEIGATGFWAMMIFLGI
                     LVVGFAYEWKKGALDWE"
     gene            1242..1718
                     /locus_tag="VFF72_08705"
     CDS             1242..1718
                     /locus_tag="VFF72_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZV93284.1"
                     /translation="MSLDGVLKEGFVTTSLDAVINWSKTGSLWPMTFGLACCAVEMMH
                     AGAARYDIDRFGMLFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLQLQAKIRRENTIAR"
     gene            1723..2319
                     /locus_tag="VFF72_08710"
     CDS             1723..2319
                     /locus_tag="VFF72_08710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZV93285.1"
                     /translation="MSSLDQLQEALMTRLGSRVGKLDRAYGELTLTVDPAAYVEVARE
                     LRDAPEFAFEELLDLCGVDYSGYRDEPWEGRRFCIVSHLLSMANNWRLRLKVFCADDD
                     VPVVDSLTGVWSSANWFEREAFDLYGVIFEGHLDLRRILTDYGFIGHPFRKDFPVYGT
                     VEMRYDPDQKRVIYQPVTIEPREIVPRVIREDNYGGLH"
     gene            2322..3575
                     /locus_tag="VFF72_08715"
     CDS             2322..3575
                     /locus_tag="VFF72_08715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZV93286.1"
                     /translation="MADIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETRTYIQSLPYMDRLDYVSMMCNEHAYCLAIEKLLGVDVPIRAQYIRVMFA
                     EITRLMNHLMFLGSHALDCGAMNVFIYCFREREDLFDMYEAASGARMHAAYFRPGGVY
                     RDLPDTMPRYRESKIRGAKATREINGNRQGSLLDFIDDFIRRFPAKVDEYETLLTDNR
                     IWKQRTVGIGVVTPERAKSLGFTGPMLRGSGVVWDLRKHQPYDVYDRVDFDVAVGVNG
                     DTYDRYLVRVEEMRQANRIIRQCVDWLKSNPGPVITANHKVAPPSRVDMKTGMEDLIH
                     HFKLFTEGFRVPEGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRPPGFAHLAALDEM
                     ARGHMIADAVAIIGTMDIVFGEIDR"
BASE COUNT          614 a         1222 c         1164 g          665 t
ORIGIN      
        1 acggcggcag cgtcaaggac gacaacgccg cttccctctt tgccgagagc gacatcgacg
       61 gcggcctgat cggcggtgcc tcgttgaacg cggcatcgtt cgtcgccatc tgtcgcgccg
      121 cggcctgacg cgcggaaaga acccatcgga gtccaggaac aatgaacacg gtgatgaacc
      181 tcgtgctggc gctgcagatc ctgacggcgc tggtgatgat cggcctggtg ctgatccagc
      241 atggcaaggg cgcggacatg ggggcgtcgt tcggcagcgg cgcctccggc agccttttcg
      301 gcgcctccgg cagcgcgaat ttcctgtccc gatcgaccgc ggtctgcgcg gccgtcttct
      361 tcgtctgcac gctggccctg gcctatgcct cggtcgatcg gccaagggcc agttcgggca
      421 gcgtcctgga caggccggcg atcggagccg cggccgccgc cagcagcgcc tccgccgtcg
      481 gtgccattcc gggagcgaaa cctttggccg ttccggcaag tggctcggcg ccggcgctcg
      541 tgcctgtcgt gccggccgcc ggcgcatcga cggccgcatc ggccgccgca tccggcgccg
      601 ccataccgca gaaataaccg cagcctcggc tagaattcgt ggttgcccgg ccagccactc
      661 ctcatgcgag ccgaagcaag aaatcgagtg ccgccgacgt ggtgaaattg gtagacacgc
      721 tatcttgagg gggtagtggc gaaagctgtg cgagttcgag tctcgccgtc ggcaccatct
      781 catgatcctc gacccttacc tccccgtcat cctcttcatc ctgatcggcg tggcagtcgg
      841 cgtcgtgccg cagatcctgg gattcctgct cggcccgcgc aagccggacc aggccaagaa
      901 ttcaccttac gagtgcggct tcgaggcgtt cgaggacgcg cgcatgaagt tcgacgtgcg
      961 ctactacctc gtcgccatcc tcttcatcct tttcgatctc gagatcgcct ttctctttcc
     1021 gtgggcagtt tcgctcaagg agatcggtgc gacggggttc tgggcgatga tgatcttcct
     1081 gggcatcctc gtcgtcggct tcgcctacga gtggaagaag ggcgccctcg attgggaatg
     1141 accatgtcga gcgaactgat ccacgtcgct gtcctccacg cgtctcgttg cggcggcggt
     1201 cgtcatggcg tcctccacgg ctcttcgtcc ctggagtccc gatgagcctc gacggcgtcc
     1261 tgaaggaggg gttcgtcacc acgagcctcg acgcggtcat caactggtcg aagaccggtt
     1321 cgctctggcc gatgacgttc ggcctggcct gttgtgcggt cgagatgatg catgccggcg
     1381 cggcccgcta cgacatcgac cgcttcggca tgctgttccg gccgagcccg cggcaaagcg
     1441 acctgatgat cgtcgccgga acgctctgca acaagatggc gccggcgctg cgcaaggtgt
     1501 acgaccagat ggccgagccg cgctgggtca tctcgatggg ctcgtgcgcc aacggcggcg
     1561 gctactacca ctacagctat tcggtcgtgc gaggctgcga ccgcatcgtg ccggtcgacg
     1621 tctacgtgcc cggatgccca ccgacggccg aagcgctcct ctacggcgtg ctgcagctgc
     1681 aggccaagat ccgtcgcgag aacacgatcg cccgctgatt ccatgagctc gctcgatcaa
     1741 ctccaggaag ccctcatgac gcggctcggc agccgtgtcg ggaagctcga tcgtgcctac
     1801 ggcgaactga cattgacggt cgacccggcc gcttacgtcg aggtggcgcg cgagctgcgc
     1861 gatgcgccgg agttcgcttt cgaagagctt ctcgatctct gtggcgtcga ctactcgggc
     1921 tatcgcgacg agccatggga agggcggcgc ttttgcatcg tctcgcacct gctgtcgatg
     1981 gccaacaact ggcggctgcg cctgaaggtg ttttgcgccg acgacgacgt gccggtcgtc
     2041 gattcgctca ccggcgtgtg gagctcggcg aactggttcg agcgcgaggc cttcgatctc
     2101 tacggggtga tcttcgaggg tcatctcgat ctgcgccgca tccttaccga ctacggcttc
     2161 atcggtcatc cgtttcgcaa ggattttccg gtctacggca ccgtcgagat gcgctacgac
     2221 cccgaccaga agcgagtcat ctaccagccg gtgacgatcg agccgcgcga gatcgtgccg
     2281 cgcgtgatcc gcgaagacaa ctacggcggc ctgcactagc catggccgac atcaagaact
     2341 acacgttgaa tttcggaccg cagcacccgg ccgcgcacgg cgtgctgcgc ctcgtcctcg
     2401 agctcgacgg cgaggtgatc cagcgtgccg atccgcacat cggcctgctc catcgcgcca
     2461 ccgagaagct cgccgagacg cgcacctaca tccagtcgct gccctacatg gaccggctcg
     2521 actacgtctc gatgatgtgc aacgagcacg cctactgcct ggccatagaa aagctcctcg
     2581 gcgtcgacgt gccgatccgc gcccagtaca tccgcgtcat gttcgccgag atcacgcggc
     2641 tgatgaacca cctcatgttc ctcggctcgc acgcgctcga ctgcggcgcg atgaacgtct
     2701 tcatctactg ctttcgcgag cgcgaagacc tgttcgacat gtacgaggcg gcgtcgggcg
     2761 cgcggatgca tgcggcctat ttccggccgg gcggcgtcta tcgcgacctg cccgacacga
     2821 tgccgcggta tcgcgaatcg aagatccgcg gcgccaaggc gacgcgcgag atcaacggca
     2881 atcgccaggg atcgctgctc gacttcatcg acgatttcat ccggcgcttc cccgccaagg
     2941 tcgatgaata cgagaccctg ctgaccgaca accgcatctg gaagcagcgt accgtcggca
     3001 tcggcgtcgt cacgccggag cgggccaaga gcctgggctt caccggcccg atgctgcgcg
     3061 gctcgggcgt cgtctgggac ctgcgcaagc accagccgta cgacgtctac gaccgggtcg
     3121 acttcgacgt ggcggtcggc gtcaacggcg acacctacga ccgctacctc gtgcgcgtgg
     3181 aggagatgcg ccaggccaac cggatcatcc gccagtgcgt cgactggttg aagtccaatc
     3241 cggggcccgt gatcaccgcc aaccacaagg tggcgccgcc gtcgcgggtc gacatgaaga
     3301 ccgggatgga agacctgatc caccacttca agctgttcac cgagggcttt cgcgtccccg
     3361 agggcgaggc gtatgcggcg gtcgagcatc cgaaaggcga gttcggcatc tacatcgtca
     3421 gcgacggcgc caacaagccg taccgcctga agatccggcc gcccggcttc gcccacctcg
     3481 ccgctctcga cgaaatggcc cgcggccaca tgattgccga tgccgtggcg atcatcggca
     3541 cgatggacat cgtgttcggg gagatcgacc gatgaacgcg gggttgttgt cgttcgggca
     3601 ggcgcgcatc gcctcgacgc tcgtcgagac ggcaaagcag cttgcaggga gggctgcatg
     3661 agccg
//
LOCUS       DASRWS010000462         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_776889, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000462 DASRWS010000000
VERSION     DASRWS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776889"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFF72_08720"
     CDS             <1..224
                     /locus_tag="VFF72_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93287.1"
                     /translation="TPQGKPLPPGIAKKLARGGSLPPGIAKRYLPRDLEVRLPPPPIG
                     VARVIVGRDVLLIRVSTGAILDMIRGVLR"
     gene            396..1703
                     /locus_tag="VFF72_08725"
     CDS             396..1703
                     /locus_tag="VFF72_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZV93288.1"
                     /translation="MRLQLNKVWSGRRGSGAPLRRLLSHLRRDRDGATAVVAALAFTG
                     VIGFAGLGSEVGLWYYKHRTMQAAVDSAALGAAQALYVSSAADYVSEARAAVARYGMV
                     DGQGGVSVAVNKPPLSGTYAGNAKAVEVIVQQPQTPLISAAFLSSGPTINARAVALEG
                     AGASGCVLALDAGASAATFGNGTTDVTLTGCSLIVDSNSSSALSLVGGASITAQSASV
                     VGGVSGVSSLHTVDGVRTHAPAVADPYANVMAPSFLGCDHTRFSAKGTVAMSPGVYCQ
                     GISINANAIVTMSPGVYFVDQGSFDVTGGATLTGSGVTIVLTSSTATNIATADIHGGA
                     VVNLSAPTSGPTAGIAFFQDRHAVPGVTNDLSGGTTQNIIGALYFPNQKVTFAGGTNT
                     GDGAGCMQIVADEIDFKGNANLAINCSGKGLKAIGGSTAKLVE"
     gene            1769..2164
                     /locus_tag="VFF72_08730"
     CDS             1769..2164
                     /locus_tag="VFF72_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZV93289.1"
                     /translation="MEFALLAPLFIGVLAAFSDLGIGAYRHMQAVEAAEAGAQSAVRQ
                     GYNSAAVEAAAAGATSLPSISTSSTQFCGCPSGTALAAVGCGTTCPDGAAAGGYVTVS
                     VATPYTALLPYPGIASPLTLSAQSTVRLQ"
BASE COUNT          370 a          769 c          721 g          360 t
ORIGIN      
        1 cgacgccaca aggaaagccg ctcccgccag ggatcgcgaa gaagctcgcc cgcggcggat
       61 cgctgccccc cggcatcgcc aagcgctatc tcccgcgcga tctcgaagtc aggctgccgc
      121 cgccgccgat cggggtcgcg cgggtgattg tcggccgcga tgtgctgctg atccgggtct
      181 cgacgggtgc gatcctcgat atgatccgcg gcgtgcttcg ctgatgcgtc ccggaggatc
      241 cgagcaatcg cggggcaaaa cgacggcaag ccgattgcaa tcaactcaat tgcgctgagc
      301 aatatcagca aaacagattg cgataactgg acaatagcga cagtaatcca ctaccgatcc
      361 catgatttgc atgggaaaag gtggtggagc ggcagatgcg actgcaactg aacaaggttt
      421 ggtctggacg acgcggatcg ggagcgccgc ttcgccgcct gctttcccac ctgcggcgcg
      481 accgcgacgg tgcgaccgca gtcgtcgcgg cgctcgcctt caccggcgtc atcggcttcg
      541 ccgggttggg ctcggaagtc ggcctctggt actacaagca tcgcacgatg caggccgcgg
      601 tcgattcggc cgcgctcggc gcggcacagg cgctctacgt cagcagtgcc gccgattatg
      661 tcagcgaggc gcgtgccgcc gtcgcccgct acgggatggt cgacggccag ggtggcgtct
      721 cggtcgccgt caacaagccg ccgttgtcgg gcacttatgc cggcaatgcg aaggccgtcg
      781 aagtcatcgt ccaacagccc cagacaccgc tgatctcggc tgccttcctc agttccggcc
      841 cgacgatcaa tgcgcgcgcg gtggcgctgg aaggggctgg agcgagcggt tgtgtactgg
      901 cgctcgacgc cggggcaagc gccgcgactt tcggcaacgg cacgaccgac gtgactttga
      961 ccggctgtag cctgatcgtc gattccaaca gtagcagcgc cctcagcctg gtcggcggtg
     1021 cgagcatcac cgcgcaatcg gcgagcgtcg tcggcggggt gtcgggagtc tcgagcctgc
     1081 ataccgtcga cggcgtcagg acgcatgccc ccgccgtcgc agatccctac gccaacgtca
     1141 tggcgccgtc gttcctcggt tgcgaccaca cgcgtttcag tgccaagggc acggtcgcta
     1201 tgtcgcccgg cgtctattgc cagggtatct cgatcaatgc gaacgccata gtgacgatga
     1261 gccccggcgt ctatttcgtc gaccaaggca gcttcgacgt gaccggcgga gcgacgctta
     1321 ccggcagcgg cgtcaccatc gtcctgacga gcagcacggc caccaacatc gccaccgccg
     1381 acatccatgg tggcgccgtc gtcaacctct cagcgccgac cagcgggccg accgccggca
     1441 tcgccttctt ccaggaccgc catgcggtgc ccggagtcac caacgacctc tccggcggca
     1501 ctacccagaa catcatcggc gcactctact ttccgaacca gaaagttacc ttcgccggcg
     1561 gcaccaacac cggcgacggc gccgggtgca tgcagatcgt ggccgacgag atcgacttca
     1621 agggcaacgc caacctggcg atcaactgct ccggcaaagg cctgaaggcg atcggcggca
     1681 gcacggcaaa gctggtggag tagggggtcc gatgacacga tggctctccc gccgcgcctc
     1741 gctgcgctgc cgccgcggca ccgcggccgt ggaattcgct ctgctcgcgc cgctctttat
     1801 cggcgtcctc gccgccttca gcgatctcgg catcggcgcc tatcggcaca tgcaggccgt
     1861 cgaggccgcg gaggccggcg cgcaatccgc cgtccgccag ggctacaaca gtgccgcggt
     1921 cgaggccgcg gctgcgggcg cgacgagcct cccctccatc tcgacatcct cgacgcaatt
     1981 ctgcggttgt ccaagcggca ccgcgcttgc cgcggtaggc tgcgggacga cctgtcccga
     2041 cggcgccgcg gccgggggtt atgttaccgt gagcgtggcg acgccataca cggccctgct
     2101 cccctatccc ggcatcgcgt cgccactgac gctgagcgcg cagtcgacgg tgcggctgca
     2161 atgaggcgca gggcgctggc cgtcctcgtt cgccgcgacg gcgcgacggc gctggagttc
//
LOCUS       DASRWS010000463         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2765864, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000463 DASRWS010000000
VERSION     DASRWS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765864"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..>2169
                     /locus_tag="VFF72_08735"
     CDS             212..>2169
                     /locus_tag="VFF72_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZV93290.1"
                     /translation="MNALYEEAGKWHAGRILSETESALQVELASGKRTKVKVASVLMR
                     FAEPAPDALVEAGAALAAEIELDLAWQFAPEGEFDFAALARDYFSAAATPVQQAASLF
                     ALFGAPHYFRRLGKGRFRKASEESVKAALLGIERKRQLAAQVDAWATEIAAGTCPAPV
                     REQIYRILFRPDKNAPEYKAVVEASRRSGRAPLDLLKDAGAIDSPYQFHWRRFLFEHF
                     PKGTGFADVDVPTIRETLPLAPVAAFSIDDSQTTEIDDALSVQGLGSGRVVVGIHIAA
                     PALAIAPGSDVDKVARDRLSTVYIPGHKLTMLPGAVVDAFTLGEGRTVPVVSLYVTVD
                     EATQAPLESRTELERIEIAANLRHDKLEDDFDEAALAAGLPASLPFASELTFLHAFAR
                     RLKAAREVVRGKPETFNRPDYAFRVDTEDGSAIVGDEPVTIVPRRRGSFLDLIVAEAM
                     ILANSTWGRWLDECGVPAIYRSQASLAPGVKVRMGTRPGPHAGMGVTHYSWATSPLRR
                     YVDLVNQWQLVACVRHGRSAALAAPFKPRDVSLLAIVADFDAAYADYNAFQSAIERYW
                     SLQFLLQKRVGELEAATLKDGLVRAAELPLVFRAVGAEPLPRGARVRVRIGAIDLLTL
                     DLAASVVARLDDDGAAAPALADVEATED"
BASE COUNT          331 a          784 c          731 g          323 t
ORIGIN      
        1 ggcgatcgag cgcagcgccg cactcgcccc gagaatctcg gcgagatacc ggcaaggccg
       61 gagctactag gggcgggagc gacgaaggcg cggcgaaccc ggtgccgcga gacattcccg
      121 ttccagggcg gcattccgca cgccaacccc gcgttccgga caggctttcg cccgacgaac
      181 cggcaccgcc ctcggcgaca atcggcgccc catgaatgcc ctgtacgaag aagccggcaa
      241 atggcacgcc ggccggatcc tgagcgagac cgagagcgcc ctgcaggtgg agctggcgtc
      301 gggcaagcgg accaaggtca aggtcgcgag cgtcctgatg cgtttcgccg agcccgctcc
      361 ggacgcgttg gtcgaggccg gcgcggcgct tgccgccgag atcgagctcg atctggcctg
      421 gcagttcgcc cccgaaggtg aattcgactt cgccgccctc gcccgcgatt acttcagcgc
      481 cgcagcgact cccgtgcagc aggccgcgtc gctgttcgcg ctgttcggcg cgccgcacta
      541 tttccgccgc ctgggcaagg gacgctttcg caaggcgtcc gaggagtcgg tcaaggcggc
      601 cctgctgggc atcgaacgga aaaggcaact cgccgcgcag gtcgatgcct gggcgacgga
      661 gatcgccgcg ggcacctgtc cggcgccggt gcgcgagcag atctaccgga tccttttccg
      721 gccggacaag aacgcgcccg aatacaaggc cgtcgtcgaa gcctcgcgcc ggtccgggcg
      781 cgcgccgctc gacctgctca aggatgcggg ggccatcgat tcgccctacc agtttcactg
      841 gcgccgcttc ctgttcgagc atttccccaa gggaacgggc ttcgccgatg tcgacgtgcc
      901 gacgattcgc gagacgctgc ctttggcgcc ggtcgccgcg ttctcgatcg acgattcgca
      961 gacgaccgag atcgacgacg cgctctcggt gcagggcctg ggaagcggac gggtcgtcgt
     1021 cgggatccac atcgccgcac cggccctggc gatcgcgccg ggatcggacg tcgacaaggt
     1081 ggcccgcgac cgcctctcca ccgtctacat ccccggccac aagctgacca tgctgccggg
     1141 cgccgtggtc gatgcgttca cccttggcga gggtcgcacc gtgccggtcg tctcgctcta
     1201 cgtgacggtc gacgaagcga cgcaggcgcc gctcgagtcg cgaaccgagc tcgagcggat
     1261 cgagatcgcg gccaacctcc ggcacgacaa gctcgaagac gacttcgacg aagccgcgct
     1321 ggccgcggga ttgcctgcgt cgctcccgtt cgcctcggag ctgaccttcc tgcatgcctt
     1381 cgcgcgacgc ctgaaggccg cacgcgaggt cgtccgcggc aagcccgaga cgttcaaccg
     1441 ccccgactac gcctttcgcg tcgacaccga ggacggcagt gccatcgtcg gcgacgagcc
     1501 ggtgacgatc gtgccgcgtc ggcgcggctc gtttctcgat ctcatcgtcg ccgaggccat
     1561 gatcctcgcc aacagcacct ggggccgctg gctcgacgag tgcggcgtgc cggcgatcta
     1621 tcgaagccag gcgagcctgg cccccggcgt caaggttcgc atgggaacgc gtcccgggcc
     1681 gcacgccggc atgggcgtga cgcactactc ctgggcgaca tcgccgctgc gacgctacgt
     1741 cgacctggtc aaccaatggc agctcgtcgc ctgcgtccgg cacggccgca gcgccgcgct
     1801 ggcggcgccg ttcaagccgc gagacgtctc gctgctggcg atcgtcgccg acttcgacgc
     1861 ggcctacgcc gactacaacg cgttccagtc ggccatcgag cgctattggt cgctgcagtt
     1921 cctgctgcaa aagcgcgtcg gcgagctgga agccgcgacg ttgaaggacg ggctcgtgcg
     1981 cgccgccgag cttccactgg tctttcgcgc cgtcggcgcc gagccgctgc cgcgcggagc
     2041 gcgcgtgcgc gtgcggatcg gcgcgatcga cctcctcacc ctcgatctcg ctgccagcgt
     2101 ggtcgcgcgc ctcgacgacg acggcgccgc cgccccggcg ctcgcggacg tcgaagccac
     2161 cgaagacga
//
LOCUS       DASRWS010000464         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3376576, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000464 DASRWS010000000
VERSION     DASRWS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3376576"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFF72_08740"
     CDS             <1..225
                     /locus_tag="VFF72_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93291.1"
                     /translation="ALGRLYDTVGIDIDPADLVRLDATVLGRKTEEVMATWARGETGG
                     GPAAPPAAAPAPAPAEEPGFFDRLFGKATN"
     gene            237..992
                     /locus_tag="VFF72_08745"
     CDS             237..992
                     /locus_tag="VFF72_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV93292.1"
                     /translation="MRKTSLCALLAVLVLQAPALAQQVGQHDAAAVPQVVRAQLVPRA
                     FTTLSSETAARVDRISHRAGEHFQKGETLVEFDCVTQRAQVAKARAVLKAAEKTYAIN
                     KRLFDMKSLSGLELEVSAAEIDKAKADVAMADAMQSKCVVEAPFAGVVVDQKAREFQY
                     TTPGQPLLEIVDDKALELEFIVPSAWLRWLKTGYAFSVAVDETGKTYHAHVELLGGRV
                     DPVSESIKVTGTIDGDAQDLMPGMSGRVVMAPP"
     gene            989..>2103
                     /locus_tag="VFF72_08750"
     CDS             989..>2103
                     /locus_tag="VFF72_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZV93293.1"
                     /translation="MSRAPQPVGPDARLLQGQVATLSLLAQLTRRARHAATVEELAFL
                     AANETHGLVPYRQAALWRRDRAGIGRVLAVSGTPMIERNAPFPMWLEQVMAALDRQRK
                     DASPLAVIASDLPADLAEDWAEWLPTHALLVPLSGGGEVRGALLLARDPPWQEGDARL
                     LSEVADAYGHAWAALDRRHRLRPWRLLMRRDRWLAIALAALVFGVMWIPVRQSALAPA
                     EIVPLEPTVVRAPLDGVVERIVVQPNQDVAAGELLLTLDPTQIRNRLDVARKARDVAE
                     AEYRQAAQQAAFDDKSRQQLTILKGRAEQRQADVAYADSLLARIQVKAEQPGIAVFDD
                     PNDWTGRPVRIGERILTIADPAKAEIEVRLPVADAINL"
BASE COUNT          319 a          760 c          739 g          285 t
ORIGIN      
        1 gccctcggcc ggctctacga caccgtcggc atcgacatcg atcccgccga cctcgtgagg
       61 ctcgacgcca ccgtgctggg ccgcaagacc gaggaggtca tggcgacctg ggcgcgcggc
      121 gaaaccggcg gcggcccggc agcaccgccc gcggccgccc cggcgccggc tccggccgaa
      181 gagccggggt tcttcgaccg gttgttcggc aaggcgacga actagcaggg ggagcggtgc
      241 gtaagacaag cctatgcgct ctcctcgccg tgctggtgct gcaggccccg gcattggccc
      301 agcaggtggg ccagcacgac gctgcggccg ttccccaggt ggtccgtgcc caactcgtgc
      361 cgcgcgcctt caccacgctg tcgagcgaaa ccgcggcccg cgtcgacagg atcagccatc
      421 gcgccggcga gcacttccag aagggcgaga cgctggtcga attcgactgc gtcacccagc
      481 gcgcccaggt cgccaaggcc cgcgccgtgc tgaaggcggc ggaaaagacc tacgccatca
      541 acaagcggct gttcgacatg aagtcgctga gcgggctgga gctcgaggtc tcggccgccg
      601 agatcgacaa ggccaaggcc gacgttgcca tggctgatgc catgcaatcc aagtgcgtgg
      661 tcgaggcgcc gttcgcgggc gtcgtggtcg accagaaggc gcgcgagttc cagtacacga
      721 cgccgggaca gccgctgctc gagatcgtcg acgacaaggc gctcgagctc gagttcatcg
      781 tgccctcggc ctggttgcgc tggctgaaga ccggctacgc cttctcggtc gccgtcgacg
      841 agaccggcaa gacctaccat gcgcatgtcg agctgctcgg cgggcgcgtc gatccggtga
      901 gcgaatcgat caaggtcacc ggcacgatcg acggcgacgc ccaggacctg atgcccggca
      961 tgagcggccg cgtcgtgatg gcgccgccgt gagccgcgcg ccgcagccag tggggcccga
     1021 cgcgcgactg ctgcaaggcc aggtggcgac gctcagcctg ctggcgcaac tcacccgccg
     1081 cgcccgccat gccgcgacgg tcgaagagct ggccttcctc gccgccaacg agacgcacgg
     1141 acttgtgccc tatcgccagg ccgcactgtg gcggcgcgat cgggccggca tcggccgcgt
     1201 gctcgccgtc tccggcacac cgatgatcga gcgcaacgcg cccttcccga tgtggctcga
     1261 gcaggtgatg gccgccctcg accgccagcg caaggacgca agccctctgg cggtcatcgc
     1321 gtccgacctg ccggcggatc tcgccgagga ctgggccgaa tggttgccga cgcacgccct
     1381 gctcgtcccg ctgtccggcg gcggcgaggt gcggggcgcg ctgctgctgg cgcgcgaccc
     1441 gccctggcag gagggcgacg cgcggctgct gagcgaagtc gccgacgcct acggccatgc
     1501 ctgggcggcg ctcgatcgcc ggcatcgcct gcggccctgg cgcctgctga tgcggcgcga
     1561 ccgctggctg gcgatcgcgc tcgccgcgct ggtgttcggc gtgatgtgga tcccggtgcg
     1621 ccagtcggcg ctggcgccgg ccgagatcgt gccgctcgaa ccgaccgtgg tgcgcgctcc
     1681 gctcgacggc gtggtcgagc ggatcgtcgt gcagcccaac caggacgtcg cggcgggcga
     1741 gctgctgctg acgctcgatc cgacccagat ccgcaaccgt ctcgacgtcg cccgcaaggc
     1801 gcgcgacgtc gccgaagccg agtaccgcca ggccgcgcag caggcggcgt tcgacgacaa
     1861 gagccgccag caactcacca tcctcaaggg ccgtgccgag cagcgtcagg ccgacgtcgc
     1921 ctacgccgat tcgctgctcg cgcgcatcca ggtcaaggcc gaacagccgg gcatcgcggt
     1981 gttcgacgat cccaacgact ggaccggccg gccggtgcgg atcggcgagc gcatcctcac
     2041 cattgccgat ccggcgaagg ccgagatcga ggtgcgcctg ccggtcgccg acgcgatcaa
     2101 tct
//
LOCUS       DASRWS010000465         4713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1892821, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000465 DASRWS010000000
VERSION     DASRWS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892821"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFF72_08755"
     CDS             <1..658
                     /locus_tag="VFF72_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZV93294.1"
                     /translation="GEHHLAPDAADCASVGTMLAGMHVAASSFAGTQPNLRGLAWWNE
                     TVPVVVPHLDPDRAALMADELAFQQHLALSSAYASLPRGAIHGDLFRDNVMFVGKTLA
                     GVFDFYFAGIDCLLFDIAVALNDWCIDLASGRLAEDRAEALVQAYDGVRRLDSAELRL
                     LPALMRSAAFRFWLSRSWDVHLPRDALVLKAHDPDHFERVLVQRRDAPWHPSRGRAPS
                     "
     gene            655..1455
                     /locus_tag="VFF72_08760"
     CDS             655..1455
                     /locus_tag="VFF72_08760"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HZV93295.1"
                     /translation="MKLIEVPPREGLVWVRRGFQVFLRQPFGFASLFAACALVFLVLL
                     RVPYVGEPILLVVAPVGSLLFMIATRLGAAGQRPVPGAFVELATADRARRIRLLQLGI
                     AYLVAAFIAIGLVAAVEGDTSGPLAEVSGDASSTTDTAATELTDPRVQAGFLLRLGLG
                     ALLSIPFWHAPGLVWWGAQGWARALFFSTLAIWRNLGAFAVYGLAWAGLGVAFAMALG
                     IVVGLAGPLSATYVATPLVLAFTTVLYASLWFTFAGCFADDDPPAVVE"
     gene            1506..2255
                     /locus_tag="VFF72_08765"
     CDS             1506..2255
                     /locus_tag="VFF72_08765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93296.1"
                     /translation="MNKVAIVTGAGSGIGRASALALLQAGWKVALAGRRADALESTRS
                     AAGDLASHALAVPTDVSDPAAVKALFAATKATFGRLDLLFNNAGRGAPGIPLEELTVE
                     QWKSVVDINLTGVFLCTQEAFRMMKSQSPRGGRIINNGSISAHAPRPHSAPYTATKHA
                     ITGLTKATSLDGRVHDIACGQIDIGNAVTEMTERMTTGMPQANGQNAAEARMDVRHVA
                     DAVVQMAGLPLDTNILFMTIMASKMPFVGRG"
     gene            2345..2599
                     /locus_tag="VFF72_08770"
     CDS             2345..2599
                     /locus_tag="VFF72_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93297.1"
                     /translation="MNADPAQPCRELALAIAVALVALPALPGCAVVSVAGTAASAAVS
                     VGGAAVAVGGAVVGSTVRVAGKVVEKTIDIAVPGGSPAPK"
     gene            2616..3311
                     /locus_tag="VFF72_08775"
     CDS             2616..3311
                     /locus_tag="VFF72_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HZV93298.1"
                     /translation="MTTTNIHFIGGEKGGVGKSLMARVLAQYLIDRQLPFLGFDTDRS
                     HGALMRFYSGYASSVTVDRIESLDAIVEAAVDQPGRRILVDLAAQTHDALATWMEDAG
                     VLSLAEELGMKLFYWHVMDSGKDSVDLLRRLLDRFGTGVQYVLVRNQVRGSDFGTLEQ
                     SGEQARAIGLGAHVVSLKKLHEGAIQKIDNASSTFWKAKSDKDGGGLGLMDRQRVKIW
                     LRDVYREIDEIGV"
     gene            complement(3281..4705)
                     /locus_tag="VFF72_08780"
     CDS             complement(3281..4705)
                     /locus_tag="VFF72_08780"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZV93299.1"
                     /translation="MAWAGCGAGTADSSTAAIGAHSAASVGASPKASIGANPPAPARS
                     VARESTAGARRPGPASRGAAYAVDLGHRIATAELAGLGREIFFDPSLSASGRIACAGC
                     HDPRHAFAPANGLAVQLAGANGDTPGLRAVPSLRYLQTVPPFSEHFFDNEGDDSADAG
                     PTGGRTWDGRAGSAHEQAALPLLSPLEMAGGTPERIVARLGAAPYAARFRTLWGAGIF
                     DHADRAFAAAAMALEVYQEGAAEFQPFSSRYDAVLRGQASLTAAQARGRAVFEDPARG
                     NCASCHPSEPGPDGAAPLFSDFGFVAIGVPRNRRLAANADPSFFDLGLCGPLREDLRD
                     RPELCGRFRTPSLRNVATRRSFFHNGVVRSLDEAVRFYASRDSDPARWYGRDRAGQPR
                     EFDDLPPRYRSNVERGAPFGGRPGGPPALDEGEVSDIVAFLGTLTDADALPAAGDERS
                     PLVGPPTTRSPKERPQTPISSISR"
BASE COUNT          655 a         1696 c         1645 g          717 t
ORIGIN      
        1 tggcgagcac catctggcgc ccgacgccgc cgactgcgcc agcgtcggca ccatgctcgc
       61 cggcatgcac gtggcggcga gcagctttgc cggcacgcag cccaatcttc gcggcctggc
      121 gtggtggaac gagacggtgc ccgtcgtcgt cccgcacctc gatcccgatc gcgccgcgct
      181 gatggccgac gagctcgcct tccagcagca tctggcgctc tcgtcggcct acgcctcgtt
      241 gccgcgcggc gccatccacg gcgacctgtt tcgcgacaac gtgatgttcg tcggcaagac
      301 gctggccggc gtgttcgact tctacttcgc cggcatcgac tgccttctct tcgacatcgc
      361 cgtggccctc aacgactggt gcatcgacct cgcctcggga cgcctcgccg aagatcgggc
      421 cgaggcgctc gtgcaggcgt acgacggcgt gcgccggctc gacagcgccg agctccgcct
      481 gctgccggca ctgatgcgca gcgccgcatt caggttctgg ctgtcgcgct cgtgggacgt
      541 ccatctgccg cgcgacgcgc tggtgctgaa ggcgcacgat cccgatcact tcgagcgcgt
      601 cctcgtccag cgtcgcgacg cgccctggca tccgtcgcgc ggccgcgccc cctcatgaag
      661 ctcatcgagg tgcctccacg agaaggcctg gtctgggtgc gacgaggctt ccaggtcttc
      721 ctgcgccagc cgttcggctt cgcgtcgctg ttcgcggcct gtgcgctcgt ctttctggtc
      781 ctgctgcgcg tgccgtacgt cggcgagccg atcctgctcg tcgtcgctcc ggtgggctcg
      841 ctgctgttca tgatcgcgac gcggctgggc gcggccggtc agcgcccggt cccgggtgcc
      901 ttcgtcgagc tcgccacggc cgaccgcgca cgccgcattc gcctgcttca gctcggcatc
      961 gcctacctgg tggccgcgtt catcgccatc ggccttgtcg ccgccgtcga gggcgacacg
     1021 tccgggccgc tcgccgaggt gtccggcgat gcctcgagca cgaccgacac ggccgcgacc
     1081 gagctgaccg acccgcgcgt gcaggccggc tttctccttc gactggggct gggcgccctg
     1141 ctgtcgatcc cgttctggca cgcgccgggc ctcgtgtggt ggggcgcgca ggggtgggca
     1201 cgcgccctct tcttcagcac cctggcgatc tggcgcaacc tcggcgcctt cgcggtgtac
     1261 gggctggcct gggccggcct cggcgtggca ttcgccatgg cgctgggcat cgtcgtcggc
     1321 ctcgccgggc cgctctcggc cacctacgtg gcgacgccgc tggtcctggc cttcaccacg
     1381 gtcctctacg ccagcctctg gttcacgttc gccggctgct tcgccgacga cgatccgccc
     1441 gccgtcgtcg agtgacacga gcctcccttc agtcccccgt tgctcgccca ccggagtttt
     1501 cgcccatgaa caaagtcgcc atcgtcaccg gcgccggcag cggcatcggc cgcgcctcgg
     1561 ccctcgccct gcttcaggcc ggctggaaag tcgccctcgc cggccggcgc gccgacgcgc
     1621 tggaatcgac gcgctcggcc gccggcgacc tggcaagcca tgcgctggcc gttccgaccg
     1681 acgtgtccga cccggccgcg gtgaaggcgc tgttcgccgc cacgaaagcg accttcggcc
     1741 gcctcgatct tctcttcaac aacgccggcc ggggcgcgcc cggcattccg ctcgaggagt
     1801 tgacggtcga gcagtggaag tcggtcgtcg acatcaatct caccggcgtg ttcctgtgca
     1861 cccaggaagc tttccggatg atgaagagcc agtcgcctcg cggcggccgg atcatcaaca
     1921 acggctcgat ctcggcgcac gcgccgcggc cgcacagcgc gccctacacg gcgaccaagc
     1981 atgccatcac cgggctgacc aaggccactt cgctcgatgg ccgcgtccat gacatcgcct
     2041 gcgggcagat cgacatcggc aatgccgtca ccgagatgac cgagcggatg acgacaggca
     2101 tgccgcaggc caacggccag aacgcggccg aagcacgcat ggacgtgcgc cacgtcgccg
     2161 acgcggtcgt ccagatggcc ggcctgccgc tcgacaccaa catcctcttc atgacgatca
     2221 tggcgagcaa gatgccgttc gtcggccgcg gctgaggacg cggcgcgtga cggattgcgg
     2281 aacgcggctg catccggaca caacgccagc gccggcccgg cgtgcatggg ccacaatgtt
     2341 tcgcatgaac gctgaccctg cccagccctg tcgcgagctg gccctcgcca tcgcggtggc
     2401 gctcgtcgcg ctgccggctc tgccgggctg cgcggtcgtc agcgtcgccg gcaccgcggc
     2461 gagcgccgcg gtcagcgtcg gcggcgcggc ggtggcggtc ggcggcgcgg tcgtcggcag
     2521 cacggtccgg gtcgccggca aggtcgtcga gaagacgatc gacatcgccg tgccgggcgg
     2581 ctcgccggcc ccgaagtgac gcacaggaac ggtcgatgac gacaacgaac atccatttca
     2641 tcggtggcga gaagggcggc gtcggcaagt cgctgatggc ccgcgtgctc gcgcagtacc
     2701 tgatcgacag gcaactgccc ttcctcggct tcgacaccga ccggtcgcat ggcgcgctga
     2761 tgcgcttcta ttccggctat gcgtcgtcgg tcacggtcga ccggatcgaa tcgctcgacg
     2821 cgatcgtcga ggccgcggtc gaccagcccg gccggcgcat cctcgtcgac ctggcggcgc
     2881 agacgcacga cgccctcgcc acctggatgg aagacgcggg cgtactgagc ctggccgagg
     2941 agctcgggat gaagctcttc tactggcacg tcatggattc gggcaaggac tcggtcgacc
     3001 tgctgcgccg gctgctcgat cgcttcggca ctggtgtgca gtacgtgctc gtccgcaacc
     3061 aggttcgcgg cagcgacttc ggcacgctcg agcaatcggg cgagcaggcg agggccatcg
     3121 gtctcggtgc ccacgtcgtc tcgctgaaga aactgcacga aggcgcgatc cagaagatcg
     3181 acaacgccag cagcaccttc tggaaggcca agtccgacaa ggacggcggc ggcctcggcc
     3241 tgatggatcg tcagcgggtc aagatctggc tgcgcgacgt ctatcgcgag atcgacgaaa
     3301 tcggcgtctg aggacgctcc ttcggcgacc gggtcgtcgg cggcccgacg agcgggctcc
     3361 gttcgtcgcc ggcggccggc aacgcgtcgg cgtcggtcag ggtgccgagg aaggcgacga
     3421 tgtcgctgac ctcgccttcg tccagggccg gcgggccgcc gggccggcca ccgaaagggg
     3481 cgccgcgttc gacgttgctt cgatagcgcg gcggcaggtc gtcgaattcg cgcggttgcc
     3541 cggcgcggtc ccgtccgtac cagcgtgccg gatcgctgtc acggctggcg tagaagcgca
     3601 cggcctcgtc gagggatcgc acgacgccgt tgtgaaagaa gctgcgccgg gtcgccacgt
     3661 tgcgcaggct gggcgttcga aacctgccgc acagctcggg gcgatcgcgc aggtcttcgc
     3721 gcaacggacc gcacaggccg aggtcgaaga acgaagggtc ggcgttggcg gcgagccgcc
     3781 gattgcgcgg cacgccgatc gcgacgaagc cgaagtcgct gaacagcggc gccgcgccgt
     3841 cgggccccgg ctcgctcggg tggcatgacg cgcagttgcc ccgcgccggg tcctcgaaga
     3901 cggcgcggcc acgcgcctgc gcggcggtca gcgacgcctg gccgcgcagc acggcgtcgt
     3961 agcgactgct gaaaggctgg aactcggccg caccctcctg gtagacctcg agggccatcg
     4021 ccgcggcggc gaaggcgcgg tcggcgtgat cgaagatgcc ggcgccccag agcgtgcgaa
     4081 agcgagccgc ataaggcgcc gcgcccagtc tggcgacgat cctttccggc gtgccgccgg
     4141 ccatctcgag cggcgagagc agcggcaacg cggcctgctc gtgcgccgag ccggcacgac
     4201 cgtcccaggt acgaccgccg gtggggccgg cgtcggcgct gtcgtcgcct tcgttgtcga
     4261 agaagtgctc gctgaaaggt ggcaccgtct gcagatagcg cagcgaggga acggcgcgca
     4321 gccccggcgt gtcgccgttc gcgccggcga gttgcaccgc caggccgttg gccggcgcga
     4381 aggcatgccg cgggtcgtgg cagccggcgc aggcgatccg gcccgatgcc gaaagcgacg
     4441 gatcgaagaa gatctcgcga ccgagccccg cgagctcggc ggtggcgatg cgatgcccca
     4501 gatcgacggc gtaggcggcg ccgcgcgaag cgggccccgg gcgtcgcgcc ccggccgtcg
     4561 attcgcgggc gacggaccgg gccggcgcgg gcggattcgc gccgatcgat gccttcggac
     4621 ttgccccgac cgacgccgcc gaatgcgcgc cgatggcggc tgtcgaagaa tcggccgtgc
     4681 cggcgccgca tccggcccat gccaggcacg aca
//
LOCUS       DASRWS010000466         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1344036, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000466 DASRWS010000000
VERSION     DASRWS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344036"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..577
                     /locus_tag="VFF72_08785"
     CDS             260..577
                     /locus_tag="VFF72_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93300.1"
                     /translation="MNRNPASTPAAVAFSEVVRSSPALTRLVDRLHESEAMLVSVRPL
                     LPAALRSAVRAGPLDDAGWSMLGANAAVAAKLRQLVPALEDALRERGHPVTTIRIKVI
                     QGS"
     gene            688..1335
                     /locus_tag="VFF72_08790"
     CDS             688..1335
                     /locus_tag="VFF72_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZV93301.1"
                     /translation="MNRRDFTSALLASGAASTLAFGVSARADAAAPVEGRDYVKVEPP
                     QETTSPGKIEVLEFFSYACPHCNAFEPTLEAWARGLPGDVALKRVPVPFLYNADNFAH
                     TYYALDALGLVSKMQMHIFSAIHVDRLRLDKPEEIAGVVAKNGGDANKFLAAFKSFSV
                     ATWVARSKKMTADYRIDGVPNLAVQGRWLTSPELAHGEAQSLQVVDFLIQRSRKG"
     gene            complement(1427..1681)
                     /locus_tag="VFF72_08795"
     CDS             complement(1427..1681)
                     /locus_tag="VFF72_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93302.1"
                     /translation="MNDTLRTVEDRYTDLLRHLASPSHVDAEQLADSLRQTEHLYSMG
                     HITSWQLQQARRAYASTLESQPRQGPPPSLATLDQEARRE"
     gene            1973..2725
                     /locus_tag="VFF72_08800"
     CDS             1973..2725
                     /locus_tag="VFF72_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZV93303.1"
                     /translation="MKSKAIVALLGLTLSAGAFAIPFVQNPTTPSVINNAPAGEENLQ
                     TIANDLFGTGAINVNTGQSSAGMFASTTPNYATSIPTLVAEYTANAGTQSFGIWFGTD
                     TDNVITYDLFLGGATPHAAAGISINGDTLSVGNASGACATAINCGMFTNPLINANSFG
                     FYFQASANAPKYFTLDQLNPGPRQDRVVSFENGTSTNWLFAYEDGTDFDYNDMAVKVE
                     SITPAVPEPETFALMLAGFGAMGFLARRRRKS"
     gene            complement(2729..2804)
                     /locus_tag="VFF72_08805"
     tRNA            complement(2729..2804)
                     /locus_tag="VFF72_08805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2769..2771),aa:Thr,seq:tgt)
     gene            complement(2902..3432)
                     /locus_tag="VFF72_08810"
     CDS             complement(2902..3432)
                     /locus_tag="VFF72_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HZV93304.1"
                     /translation="MTTPIAPATAGTSTRPYLIRALHDWCTDNGFTPYIAVFVDDAVR
                     VPKEYVKNSEIVLNVGFEATSALKLGNETIEFKARFGGSSREIVVPVDHVIAIYAREN
                     GQGMAFPMPTGSVPATAGPEGAAPESKPPVPRGPRLARSEPHAPAEAKLPKATSEDPD
                     PPTQPPPKRPALKRVK"
     gene            complement(3457..>4047)
                     /locus_tag="VFF72_08815"
     CDS             complement(3457..>4047)
                     /locus_tag="VFF72_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93305.1"
                     /translation="TTDPYSHRCRFVLFEKGMDFEIRDVDLFAKPEDIALMNPYNEVP
                     ILVERDLILYESHIINEYIDERFPHPQLMPGDPVARARVRLFLFNFEKELFAHVNVLE
                     GRGSKGTDKQLEKARSQIRDRLTQLAPIFLKNKYMLGDDFSMLDVAIAPLLWRLDYYG
                     IDMSKNAVPLLKYAERIFSRPAYIEALTPSEKVMRK"
BASE COUNT          704 a         1330 c         1278 g          735 t
ORIGIN      
        1 cttctgaacg accgaccggt attgcctcag caaccggtcg ttgcggctgc cgaagatcga
       61 ggtgagaagc ttgggcatgc gagtcagtgt ggagaggtca tgagcgcccg tccggccgca
      121 tggcgcggag cgggacgacg cggcgtcacg cccgtcggaa actgtggcct cgctcccgaa
      181 aagcaaggtg cagcgcgggc gcgaaagggc gcgcaaaacc gcaaatttta acattcggcg
      241 gccgcattag actttcgagg tgaaccgcaa tcccgcgtcg acccctgctg ccgtcgcatt
      301 ctccgaggtg gtccgatcga gcccggcgct gacccggctg gtcgaccgtc tgcacgaatc
      361 cgaggcgatg ctggtcagcg ttcgtccgct gctacctgct gccctgcgca gcgctgtccg
      421 ggccggcccg ctcgacgatg ctggatggtc gatgctgggt gccaatgcgg ccgtcgccgc
      481 caagcttcgc cagctcgtgc cggccctcga agatgcgctg agagagcgtg gccacccggt
      541 gacgacgatc cgcatcaagg tcatccaggg ctcgtgagac ggccgcctgt gacggcaatc
      601 gtgcctgtcg gccggcacgg ccctcgccgc gttgaacttc gtgccaccat tcgactcctc
      661 gcagaactcc tgatggaccg cactctcatg aaccgacgcg atttcacctc tgccctgctt
      721 gcttccggcg ccgcctccac cctcgccttc ggtgtttcgg cgcgggccga tgccgccgca
      781 cccgtcgagg gtcgcgacta cgtgaaggtc gaaccgccgc aggaaaccac gagcccgggc
      841 aaaatcgagg tgctcgaatt cttttcctat gcctgtccgc actgcaacgc gttcgagccg
      901 acgctcgagg cgtgggcgcg cggactgccg ggtgatgtgg ccctcaagcg ggttccagtg
      961 ccgtttctgt acaacgcgga caacttcgcc cacacctatt acgccctcga cgcgctgggc
     1021 ctcgtttcca agatgcagat gcacatcttt tcggcaatcc acgtcgatcg ccttcggctc
     1081 gacaagcccg aagagatcgc tggcgtcgtc gcgaagaacg gcggtgacgc gaacaaattc
     1141 cttgctgcct tcaagtcatt ttccgtggcg acctgggtcg cccgctcgaa gaagatgact
     1201 gccgactacc gcatcgacgg cgttcccaac ctggccgtcc agggacgctg gctgacctcg
     1261 cccgagctcg cccacggcga agctcagtcg ctccaggtcg tcgatttcct gatccagcgc
     1321 tcgcgaaagg gctgagaggc tggctgacgc gcggcaatgg ccgcggcagt tgccccggca
     1381 cgccgccttc gtcgaagagc cctttcacga gagcggtccg accggactat tcccttctcg
     1441 cctcctgatc gagggtcgcc aacgaaggcg gcggcccttg gcgcggttgc gactcgagcg
     1501 tcgacgcgta ggccctgcgc gcctgctgga gttgccagga ggtgatgtgg cccatcgagt
     1561 agagatgctc ggtctgccga agcgaatcgg ccagttgctc ggcatcgacg tgcgaaggac
     1621 tggccaggtg acgaagcaga tcggtgtagc gatcctcgac tgttcgaagc gtgtcattca
     1681 ttgcacatcc accacggtat gtaacgaggt cattccagcc tagcgccacg gcgttgcgac
     1741 gatcgtccgc gatgaaccgg gtggcccgac agcgcttgcc gccccgtgat gtagggcttc
     1801 gccgcatgga tcgaaccttg tcctgcagca cctcttgcgt ggggccagtg agggcgcacg
     1861 cgcgaccgtt cgtcggcgac cccgtgaacg ggggatgtgc cacctgtcgt cgatcgatag
     1921 atttcgctcc agcgccgcgc cgcggcccat gcgtcacctg gagtcaccgt tcatgaaatc
     1981 gaaagccatc gtcgccctgc tgggcctgac cctcagcgcc ggcgccttcg ccattccgtt
     2041 cgtgcagaac ccgaccaccc cgtcggtgat caacaatgcc ccggccgggg aagagaattt
     2101 gcaaaccatt gccaatgacc tgttcggcac gggtgcgatc aacgtcaaca cgggccagag
     2161 ctcggccggc atgttcgcct cgacgacgcc gaactacgcc accagcatcc cgacgctggt
     2221 cgccgaatac accgccaacg caggaacgca gtcgttcggc atctggttcg gcacagacac
     2281 cgacaacgtc atcacgtatg acctgttcct gggcggagcc acgccgcacg ctgcggctgg
     2341 catcagcatc aacggcgaca cgctgagcgt cggcaacgcg tccggagctt gcgccacggc
     2401 gatcaactgc ggcatgttca cgaatccgct gatcaacgcg aattctttcg gcttctactt
     2461 ccaggcgtcg gccaacgcgc cgaagtactt caccctcgac cagttgaacc cgggtccgcg
     2521 ccaggaccgc gtcgtctcgt tcgagaacgg cacgtcgacg aactggctct tcgcctacga
     2581 agacggcacc gacttcgact acaacgacat ggccgtcaag gtcgaatcga tcacgccggc
     2641 ggttcccgag cccgagacct tcgcgctcat gctcgccgga ttcggtgcga tgggcttcct
     2701 ggcccgcagg cgccgcaagt cctgatgctg gtgccggctg tccggatcga acggacgacc
     2761 ttccgcttac aaggcgggtg ctctaccaac tgagctaagc cggcatcgac ctcgtctgcc
     2821 acgctgccga gccgcggcag tataggttgc aaccaacaag ggccatggcc gccgccggca
     2881 gccacggccc ttcgcttttg cctacttgac gcgcttgagc gccgggcgct tcggtggcgg
     2941 ctgcgtcggc ggatcggggt cctccgacgt ggccttcggc agcttcgcct cggcaggcgc
     3001 atggggctcc gagcgcgcca gccgcggccc gcgcggcacc ggcggcttcg actctggcgc
     3061 cgcaccttcc gggcccgcag tcgccggcac cgatcctgtc ggcatcggga aggccatgcc
     3121 ctgaccgttc tcgcgtgcat agatggcgat cacatgatcg accggaacga cgatctcgcg
     3181 ggagctgccg ccgaatcgcg ccttgaattc gatcgtctcg ttgcctagct tgagcgcgct
     3241 ggtggcctcg aagccgacgt tgaggacgat ctcgctgttc ttcacgtatt ccttgggcac
     3301 gcggacggcg tcgtcgacga agacagcgat gtacggcgtg aagccgttgt ccgtgcacca
     3361 gtcatgcagc gcacggatga ggtacggccg cgtcgacgtg ccggcagtgg cgggagcgat
     3421 cggcgtcgtc atgacaatga agccgcgatt cgaacgctac ttgcgcatca ccttttcgga
     3481 aggcgtcagc gcctcgatgt aggccgggcg cgaaaaaatg cgctcggcat acttgagcag
     3541 cggcacggca ttcttggaca tgtcgatgcc gtagtagtcg agccgccaga ggagcggcgc
     3601 gatcgcgacg tcgagcatcg agaagtcgtc gccgagcatg tatttgttct tcaggaagat
     3661 cggcgcgagc tgcgtcagcc ggtcgcggat ctgcgagcgg gccttctcga gttgcttgtc
     3721 ggtgcccttg ctgccgcgac cctcgagcac gttgacgtgg gcaaacagct ccttctcgaa
     3781 attgaaaagg aagaggcgca cccgggcgcg cgccaccgga tcgcccggca tgagctgggg
     3841 atgcgggaac cgctcatcga tgtattcgtt gatgatgtgc gactcgtaca ggatgaggtc
     3901 acgctcgacg aggatgggca cctcgttgta cgggttcatc agcgcgatgt cttcgggctt
     3961 ggcgaacagg tcgacgtcgc ggatctcgaa atccatgccc ttttcgaaca gcacgaagcg
     4021 gcaacggtgc gaatagggat cagtggt
//
LOCUS       DASRWS010000467         4743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2462352, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000467 DASRWS010000000
VERSION     DASRWS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462352"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFF72_08820"
     CDS             <1..782
                     /locus_tag="VFF72_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93306.1"
                     /translation="DFNDLLDADLAPEVRGAGGEWWSLRAWMEHGALPYGAERLRDAI
                     FDHQPFEIKQHIVNFIAEELRHHEASVLVAKSLAAYQEVPRADYFKAVIPRFHDERDE
                     KAMSFFAGLAVNTLFEQLSGELLQARYENARYESIRRACQLILRDEARHIQFGRIVMR
                     RFFAELPADDKRMLGEKVAKKLRGSLLSGVYAVVNLPPDERERAGRNRALACEHGLGA
                     THPDAEMAIIKRALDQIRADVAPYGVVIPQVAEVDSPATVH"
     gene            800..1834
                     /locus_tag="VFF72_08825"
     CDS             800..1834
                     /locus_tag="VFF72_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV93307.1"
                     /translation="MTMTMTMTMTKSASARGGTLRRRAFVVATIAGLAALPFVSPVEA
                     QPAYPSHAITLVVPYPAGGSADVLARAIGQRLGERLHQAVVVENHAGAATAIGARDVA
                     RAAPDGYTLLLGTVSSQAINPAMNKVGYDPVADFTAIAPIGSIPFVLVANPQAHLDSV
                     ADVIARAKAKPGELSYASAGIGTSNHLAGELFASDAHIKMLHVPYKGSAPALTDVLAG
                     HVPLMFDLQATSLAHVRAGRLKALAVTSPQRSELLPDVPTMSESGLPGYEVSAWFGVF
                     APAGVPKPIVEKLNAEISAIISSPEMKKRLQDLGVSADVATAPEYAAFVRREAAKWAA
                     TVKSAGLAPS"
     gene            1831..2718
                     /locus_tag="VFF72_08830"
     CDS             1831..2718
                     /locus_tag="VFF72_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZV93308.1"
                     /translation="MTDIAATLADIGPRWHLDIRAHSQQVKDAYEPILARAPRGDLTV
                     FRDIAYGPHGRQVLDVFAPAAALAGAPVVAYVHGGAFLRGDKRTTDQIYDNVLFWFAR
                     QGFVGVNVEYRHAPQATYPGGAEDVAAAMAWVQQRIAGFGGDPARVLLIGHSAGGTHA
                     ATYAFDPAVAAVPPQIAALALVSARLRADRLAANPNAAGVAAYFGADASSDDARSPVS
                     HAARHPELPVMIAVAEFENPLLDVYGIEFAQRRAAALGRAPRFVQMPGHNHMSIVAHF
                     NSGEETLGREILDFFSRVG"
     gene            2798..4381
                     /locus_tag="VFF72_08835"
     CDS             2798..4381
                     /locus_tag="VFF72_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZV93309.1"
                     /translation="MPDMLPTPTPFDQDFGLVADMIRLHARQDPARLALVDARDRLDY
                     GELDATMDRVAAALQRDGVRSGESIAICAAASVNYAAVFLGALRAGVVVAPLAPGETA
                     KSLAGMLADSGARRLFVDDSTAASFGATSDVPRTMLDGGSASGALETWLAPRGSVPHD
                     VVIAADAPFNIIYSSGTTGTPKGIVQPHAMRWAQVRRGGGAYGFGADATTLLATPLYS
                     NTTLVVFLPSLAWGGTVVLMARFAAEGYLRLAEAHRVTHTMLVPVQYQRIMAEPAFDR
                     FDLSSYRMKFCTSAPFAAALKADVLARWPGGLVEFYGMTEGGGSCALLAHQHPDKLHT
                     VGQPMDGHDIRLVDDDGRQVAPGAIGEIVGRSGSMMLGYHRQAAKTRDAEWTSPEGLR
                     FIRTGDVGRFDADGFLMLLDRKKDMIISGGFNIYPSDLEAVLREHAVVAEASVVGVPS
                     ARWGETPVAFVVVRRPTPDSPVAGAAAEGADLLAWFNARVGKTQRLAALHIVDELPRS
                     AIGKVLKRELRERHAATGS"
BASE COUNT          695 a         1709 c         1618 g          721 t
ORIGIN      
        1 tagatttcaa tgacctgctg gacgcggacc tcgcgcccga ggtgcgtggg gcaggcgggg
       61 aatggtggag cctgcgcgcc tggatggagc acggcgcgct gccttacggg gccgagcggc
      121 tgcgcgatgc gatcttcgat caccagccct tcgagatcaa gcagcacatc gtcaacttca
      181 tcgccgagga gctgcgccat cacgaggcct cggtgctcgt cgccaagagc ctggccgcct
      241 accaggaggt gccgcgcgcc gactacttca aggcggtgat cccgcgcttc cacgacgagc
      301 gcgacgagaa ggcgatgagc tttttcgccg gcctggcggt caacacgctg ttcgagcagc
      361 tctcgggcga gctgctgcag gcgcgctacg agaacgcccg ctacgaatcg atccgccgcg
      421 cctgccagct gatccttcgc gacgaagcgc ggcacatcca gttcggccgc atcgtcatgc
      481 gccgcttttt cgccgagctg cctgccgacg acaagcgcat gctcggcgag aaggtcgcga
      541 agaagctgcg cggcagcctg ctgagcggcg tctatgccgt cgtcaacctg ccgcccgacg
      601 agcgcgagcg cgccggccgc aaccgcgccc tggcgtgcga gcacggcctg ggcgccaccc
      661 accccgacgc ggagatggcg atcatcaagc gcgccctcga ccagatccgc gccgacgtcg
      721 cgccgtacgg tgtcgtgatt ccgcaagtgg cggaagtgga ttcgccggcg acggtccact
      781 gacgaaggaa gcgaagacga tgacgatgac gatgacgatg acgatgacga aatccgcctc
      841 cgcgcgcgga ggcacgctgc gccggcgcgc ctttgtcgtg gccaccatcg ctggcctggc
      901 cgcgctgcca ttcgtgtcgc cggtcgaggc ccagcccgcc tacccgagcc acgccatcac
      961 cctggtcgtg ccgtaccccg ccggcggttc ggccgacgtg ctggcccggg cgatcgggca
     1021 acggctcggc gagcgcctgc accaggccgt cgtcgtcgag aaccacgccg gcgcggcgac
     1081 ggcgatcggc gccagggacg tggcgcgcgc cgcgcccgac ggctacacgc tgctcctcgg
     1141 caccgtgagc tcgcaggcga tcaacccggc gatgaacaag gtcggctacg acccggtcgc
     1201 cgacttcacg gcgatcgctc cgatcggctc gattcctttc gtgctcgtcg ccaatccgca
     1261 agcgcacctc gacagcgtcg ccgacgtgat cgcccgggcg aaggcgaagc ccggcgagct
     1321 gtcctatgcg tcggccggca tcggcacctc gaaccatctc gccggcgagc tgttcgcgag
     1381 cgacgcccac atcaagatgc tgcacgtgcc gtacaagggc agcgccccgg cgctcaccga
     1441 cgtgctcgcc ggccacgtgc cgttgatgtt cgacctgcag gccacgtcgc tggcgcacgt
     1501 cagggcaggg cgcctgaagg cccttgcggt gacgagcccg cagcgaagcg agctgctgcc
     1561 cgacgtgccg acgatgagcg agagcggcct gcccggctac gaggtctcgg cctggttcgg
     1621 cgtcttcgct cctgccggcg tgccgaagcc gatcgtcgag aagctcaacg ccgagatcag
     1681 cgcgatcatc tcctcgccgg agatgaagaa gcggctgcag gacctgggcg tcagcgccga
     1741 cgtggcgacg gcacccgaat acgccgcctt cgtcaggcgc gaggccgcga aatgggcggc
     1801 gacggtgaag agcgccggcc tcgcgccgtc gtgaccgaca tcgcggcaac gctcgccgac
     1861 atcgggccgc gctggcatct cgacatccgg gcccacagcc agcaggtcaa ggacgcctac
     1921 gagccgatcc tggcgcgggc gccgcgcggc gacctcaccg tctttcgcga catcgcctac
     1981 gggccgcacg ggcgccaggt gctcgacgtg ttcgcccccg ccgccgccct tgccggggcg
     2041 ccggtcgtcg cctacgtcca cggcggcgcc ttcctgcgcg gcgacaagcg caccaccgat
     2101 cagatctatg acaacgtcct tttctggttc gcccgccagg gcttcgtcgg cgtcaacgtc
     2161 gagtaccggc acgcgccgca ggcgacgtat cccggcggcg ccgaggacgt ggccgcggcg
     2221 atggcgtggg tccagcaacg catcgccggg ttcggcggcg atccggcgcg cgtccttctc
     2281 atcggtcact cggccggcgg cacgcatgcg gccacgtacg cgttcgaccc ggccgtagca
     2341 gcggtgccgc cgcagatcgc cgcgctcgcg ctcgtctcgg cccgcctgcg cgccgaccgt
     2401 ctcgccgcca atccgaacgc cgcgggcgtc gccgcctact tcggcgccga cgcatcgagc
     2461 gacgacgcgc gctcgccggt aagccacgcc gcgcgccacc ccgagctgcc ggtgatgatc
     2521 gccgtcgccg aattcgagaa cccgctcctc gacgtctacg gcatcgaatt cgcgcagcgc
     2581 cgggccgcag cgctcggacg ggcgcctcga ttcgtgcaga tgcccggcca caaccacatg
     2641 tcgatcgttg cccacttcaa cagcggcgag gagactctcg gccgggagat cctcgacttc
     2701 ttctccaggg tcggctagga gacactgcga cgcgtcggcg atggcggcgc cgcagagagt
     2761 ttgtctccgt cgacatcggc ccagcgccgc acggacaatg cccgacatgt tgcccacgcc
     2821 cacgcctttc gaccaggact tcggcctcgt cgccgacatg atccggctgc acgcgcggca
     2881 agatccggcg cgtctggcgc tcgtcgatgc ccgggatcgg ctcgactacg gcgagctcga
     2941 cgcgacaatg gaccgggtcg ccgcggcgct gcagcgcgac ggcgtgcgca gcggcgaatc
     3001 gatcgcgatc tgtgccgcgg cgtcggtgaa ctacgcggcc gtcttcctcg gcgccttgcg
     3061 ggcgggcgtt gtcgtggcgc cgctcgctcc gggggagacg gcgaagagcc tcgccggcat
     3121 gctcgccgat tcgggcgccc gtcggctctt cgtcgacgac agcacggcgg cgtcgttcgg
     3181 cgcgacgagc gacgtgccgc gcaccatgct cgatggcggc tcggcgagcg gcgcgctcga
     3241 aacctggctc gcaccgcggg gttcggttcc gcatgacgtg gtgatcgcgg ccgacgcgcc
     3301 cttcaacatc atctattcgt cgggcaccac cggaacgccc aagggcatcg tccagccgca
     3361 tgcgatgcgc tgggcccagg tgcggcgtgg cggcggcgcc tatggcttcg gtgccgacgc
     3421 gacgacgctg ctggcgacgc ctctctactc gaacaccacg ctcgtcgtct tcctgccgag
     3481 cctcgcctgg ggcggcaccg tcgtgctgat ggcgaggttc gcggccgaag gctacctgcg
     3541 cctcgccgag gcgcatcgcg tcacccacac catgctcgtt ccggtgcagt accagcgcat
     3601 catggccgag ccggccttcg accgcttcga cctgtcgtcg tatcggatga agttctgcac
     3661 cagcgcgccc ttcgccgcgg cgctcaaggc cgacgtgctg gcgcgctggc cgggtggcct
     3721 cgtcgagttc tacggcatga ccgaaggcgg cggcagttgt gcgctgctgg cgcaccagca
     3781 tcccgacaag ctgcatacgg tcggccagcc gatggacggt cacgacatcc gcctcgtcga
     3841 cgacgacggt cgccaggtgg cgccgggcgc catcggcgag atcgtcggtc gctcgggctc
     3901 gatgatgctc ggctaccacc ggcaggcggc gaagacgcga gacgccgaat ggacgagccc
     3961 cgagggactt cgcttcatcc gcaccggcga cgtcggccgc ttcgatgccg acggcttcct
     4021 gatgctcctc gatcgcaaga aggacatgat catcagcggt ggcttcaaca tctacccgag
     4081 cgatctcgag gcggtgctgc gcgagcatgc cgtcgtcgcc gaggcgtccg tcgtcggcgt
     4141 gccgtcggcg cgctggggcg agacgccggt ggctttcgtc gtggtgcggc gaccgacccc
     4201 ggattcgccg gtcgctggcg ccgccgccga aggggcggat ctgctcgcct ggttcaatgc
     4261 gcgcgtcggc aagacgcagc ggctggccgc actgcacatc gtcgacgagc tgccgcgcag
     4321 cgcgatcggc aaggttctca agcgcgagct tcgcgaacga cacgcggcga cggggtcctg
     4381 attcgccgcg tcgtgcgacg cgcgtcgctt tcgtccgtcc tcggccgatc cgtcggcgac
     4441 cggctgaagt ccggccaagc gctttgccct caggcggatg tcgactctcc tggcatccag
     4501 ggcggccgtc gcccgcgggc tggcgcgtcg tgtccaatgc ggtctgtttc gctgcctcgg
     4561 agccacgcat gaaagtcagg aagatcgccc tcggattgct ggcggtcatc gtcgttgccg
     4621 gcgttgccgg ctggttcagc ctcgacaagg agacgcgcgg gctgctggcg acgttcccga
     4681 ccaacacgga tctgctgttc tggagtcagc gacagcgcga cgcggccttt cgcgcgctcg
     4741 aca
//
LOCUS       DASRWS010000468         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2768643, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000468 DASRWS010000000
VERSION     DASRWS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768643"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFF72_08840"
     CDS             <1..473
                     /locus_tag="VFF72_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93310.1"
                     /translation="ADPGASRVVRHDDLDDRPLQIETMNQQLRMEKRRGDSLLHLYPD
                     EAAHHAARLRDLENADKPLRAADERIANLAREQRRLRDASALWAGKALPSELRERSQR
                     VDVALQLERTRLKACREERELVGKAYDDELVVLRRLWAAQGRTVSAVDDVSRAN"
     gene            complement(525..791)
                     /locus_tag="VFF72_08845"
     CDS             complement(525..791)
                     /locus_tag="VFF72_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HZV93311.1"
                     /translation="MDSASRLDESLGTLRSEIRALGPDDGEARQRLEGLVADISAMQG
                     PDGVVTGELPTGQIEALILDFEMSHPRVAVVVNEVLEKLSAMGI"
     gene            complement(882..1304)
                     /locus_tag="VFF72_08850"
     CDS             complement(882..1304)
                     /locus_tag="VFF72_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZV93312.1"
                     /translation="MGKPLIGALLRFPREVVVERMLEAVNAGGHDLSAAELNMLMYPG
                     PDGKRPSALARECGMSRQSVNYLLAGLEARDYLRRADGESGVARVVHLSPRGRSVGLL
                     MRKTVVQIEREWTKVLGTERFEALRDTLVDLSRALGKL"
     gene            1416..1877
                     /locus_tag="VFF72_08855"
     CDS             1416..1877
                     /locus_tag="VFF72_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZV93313.1"
                     /translation="MTASPTPAAADPIRIGQIEIRYLQTADDGCGVGCFEMIVPPGAA
                     VPPPHSHAACEELMYGLDGILRCTVGSETRDLGPGDVMGTPKGVPHAFSNPHVAAARV
                     LVVNSPGIDPQYFRDIASIVSRGGPPDRALMAGTMHRYGLTPVAPPPGPPR"
     gene            complement(1874..3109)
                     /gene="boxA"
                     /locus_tag="VFF72_08860"
     CDS             complement(1874..3109)
                     /gene="boxA"
                     /locus_tag="VFF72_08860"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HZV93314.1"
                     /translation="MCIRCNTCEATCPVGAITHDDRNYVVRADVCNQCMACISPCPTG
                     SIDNWRTVPKALAYPIEAQLGWDELPAELTPEQMEAAGVEADTAIASAQAASAVMAVA
                     PESAAGEAPFNSASFGSTVPPWSAAHAYTNLHGPKSPTTATVAGNVNCTEAGFENQTH
                     HIVLDFGAMPFPVLEGQSIGIVAPGLDERGKPHVARQYSVASPRNGERPGYNNVAITV
                     KRVLEDHQGRTVRGVASNYVCDLKVGDTVQVIGPFGQSFLMPNHPRSHIVMICTGTGS
                     APMRAMTEWRRRLRKTGKFEGGKLMLFFGARTREELPYFGPLTSLAKDFIDVNLAFSR
                     TPGQPKRYVQDLMRERAADLALLLADPNAHFYVCGLKAMEEGVLLAMRDVAAQKSLDW
                     DGAIAPALKREGRLHMETY"
BASE COUNT          475 a         1084 c         1068 g          484 t
ORIGIN      
        1 acgccgatcc gggcgcatcg cgagtggtca gacacgacga cctggacgac cggccgctac
       61 aaatcgagac gatgaaccag cagctgcgca tggagaagcg aagaggcgac agcctgctgc
      121 acctctaccc ggacgaggcc gcgcaccacg ccgcccgact tcgtgatctc gagaacgcgg
      181 acaagcccct ccgcgccgcc gatgagcgca tcgcgaatct cgcgcgcgag caaaggcgcc
      241 ttcgcgacgc gtcggcgctc tgggcaggca aggcgctgcc gtcggagctc agggagcgat
      301 cgcaacgcgt cgacgtcgcg ctgcaactcg agaggaccag gctgaaggcc tgtcgcgagg
      361 agcgagagct tgtcggcaag gcctatgacg acgagctcgt cgtgctgcgc aggctctggg
      421 ctgcccaggg aaggaccgtg agcgccgtcg acgacgtctc ccgggcgaac tgagctcggc
      481 gcgggaccgg cggcgatggc gcggcgaccg cgcctcgcct ggggtcagat ccccatcgcg
      541 ctcagcttct cgaggacctc gttcacgacg accgcgacgc gcggatggga catctcgaaa
      601 tcgaggatca aggcctcgat ctgcccggtc ggcaactcgc cggtgacgac gccgtccggg
      661 ccctgcatcg ccgagatgtc ggcgacgagc ccttccagac gctgccgggc ttccccgtcg
      721 tcggggccga gcgcgcggat ctccgagcgc agggtgccga gcgattcatc aagacggctg
      781 gcactgtcca tggcgagctc ctctgcgaac gacgatgccg caatggctcc attctcgagc
      841 acgcggcctt ccggcaccgg gccatgccgg cggtgcgcct tctacagctt gccgagggcg
      901 cgggacaagt cgaccagcgt gtcgcggagc gcttcgaatc gctccgtgcc gaggaccttc
      961 gtccattcgc gctcgatctg cacgacggtc ttgcgcatca gcaggcccac ggaccggccg
     1021 cgcggcgaca gatggacgac gcgcgcgacg ccgctttcgc cgtcggcacg acgcaggtag
     1081 tcgcgggcct cgaggcccgc gagcaggtag ttcaccgact ggcggctcat gccgcactcg
     1141 cgcgcgagcg cgctcggccg cttgccgtcc gggcccgggt acatcagcat gttcaactcg
     1201 gcggcggaaa ggtcgtggcc gccggcgttg accgcctcga gcatgcgctc gaccaccacc
     1261 tcgcgcggga agcgcagcaa cgcgccgatc agcggcttgc ccaagccggc cgcgatctcg
     1321 gccctggcag attccgacgg cctcggcttg cttgacggcg acgtaaagct tttgtacatt
     1381 gacgtataga atttttacgc gcaaaggagt ttgccatgac tgccagcccg acgccagcgg
     1441 cagccgatcc gatccggatc ggccagatcg agatccggta tctgcagacc gctgacgacg
     1501 ggtgcggcgt cggctgcttc gagatgatcg ttccgccggg cgccgcggtg ccgccgccgc
     1561 acagccatgc cgcgtgcgag gagttgatgt acgggctcga cggcatcctt cgttgcacgg
     1621 tggggagcga gacgcgcgac ctcggccccg gcgacgtcat gggcactccg aagggcgtgc
     1681 cgcacgcctt cagcaacccg cacgtcgcag ccgcccgcgt gctggtggtg aactctcccg
     1741 gcatcgaccc gcagtacttt cgcgacatcg cctcgatcgt gagccgcggc ggcccgcccg
     1801 atcgcgccct gatggcgggc acgatgcatc gctacgggct gacgccggtc gcgcccccgc
     1861 ccggaccgcc gcgctaatac gtctccatgt gcagccgccc ttcccgcttg agcgccggcg
     1921 cgatcgcgcc gtcccagtcg aggctcttct gcgccgcgac gtcgcgcatc gcgagcaaca
     1981 cgccctcctc catcgccttc agcccgcaga cgtagaagtg cgcgttcgga tcggcgagca
     2041 gcaacgccag gtcggccgcg cgctcgcgca tcaggtcctg cacgtatcgc ttcggctgcc
     2101 ccggcgtccg tgagaacgcc aggttcacgt cgatgaaatc cttcgccagc gaggtcagcg
     2161 ggccgaagta aggcagctct tcgcgggtgc gcgcgccgaa gaagagcatc agcttgccgc
     2221 cttcgaactt gccggtcttc ctcaggcggc ggcgccactc ggtcatcgcg cgcatcggcg
     2281 cgctgccggt cccggtgcag atcatgacga tgtgcgacct ggggtggttg ggcatcagga
     2341 agctctggcc gaaggggccg atcacctgca cggtgtcgcc caccttcagg tcgcacacgt
     2401 agttgctggc gacgccgcgc acggttcggc cctggtggtc ttcgagcacg cgcttcacgg
     2461 tgatggcgac gttgttgtag cccggccgct cgccgttgcg cgggctggcc acgctgtact
     2521 ggcgcgcgac gtgcggcttg ccgcgctcgt cgagtcccgg cgccacgatg ccgatcgatt
     2581 gcccctcgag caccgggaag ggcatggcgc cgaagtcgag cacgatgtga tgcgtctggt
     2641 tctcgaagcc ggcctcggtg cagttgacgt tgcccgccac cgtggccgtg gtcgggctct
     2701 tcgggccatg caggttggtg taggcgtgcg cggccgacca gggcggcacc gtggagccga
     2761 aggaggcgct gttgaacggc gcctcgccgg cggccgattc gggcgccacg gccatcaccg
     2821 cggacgcagc ctgcgccgag gcgatggccg tatcggcttc cacgcccgcc gcttccatct
     2881 gctcgggcgt cagctcggcg ggaagctcgt cccagccgag ctgcgcctcg atcggatagg
     2941 ccagcgcctt cggcacggtg cgccagttgt cgatcgagcc ggtcgggcac ggcgaaatgc
     3001 acgccatgca ctggttgcag acatcggcac gcacgacgta gttgcggtcg tcgtgcgtga
     3061 tcgcgccgac cgggcaggtc gcctcgcagg tgttgcagcg gatgcagatc t
//
LOCUS       DASRWS010000469         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3378762, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000469 DASRWS010000000
VERSION     DASRWS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378762"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFF72_08865"
     CDS             complement(<1..584)
                     /locus_tag="VFF72_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZV93315.1"
                     /translation="MRGPFVAQFPPPGTIAAMPRLAANCSFLFPERPFLDRFEHAARA
                     GFAAVEFLFPYAHPAPDIRARLQAHGLRAVLFNTPPGDAAGGERGMACIPGREAEFRD
                     GALRALDYAQALGVGNVHVLAGIVPDGVGADAARATYVANLAFAAARFAEARVRVLVE
                     PLNRFDAPGYFVNRSAQAISLIDEIGAANVFLQY"
     gene            653..2653
                     /locus_tag="VFF72_08870"
     CDS             653..2653
                     /locus_tag="VFF72_08870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZV93316.1"
                     /translation="MPSSVKRPIRLGIQPRMLLVALAIGSLFLAYIAVTTVRQASRDR
                     AQAGEEMRLVAALAGARLDDHIGDATQLLNALAGTLTVDVDDTAVNDATLGSLAPQLP
                     ASVSDVSLWTIDGTNIGSSETSPAMPRRNASDHEFLAAALRADGVAIEAPLRWRKGGD
                     WTAVFAHRVVRDKKVIGVVSVETPLSALPRMLDPEGTLPAGAVITLVDAQGRIVARSL
                     DAERWIGQPAPLDPVELQQRMAMGRGSAENDGLDGKPRVFGFARARGMPWLVYVGMPV
                     DVALASANGNTLQSLAMGLGMLAVGLLLAAWVASRITRPLRQLSADARLLGEGRFEHR
                     SAVRTGSEIGLLAHTLNRMAEALQERIAAARRSAERLGLALEGSNQALFDWDIAGRRV
                     HYSARASMLRGGPDEEIDASPEEMRAFVHPDDLPTVLGRLTDAVRGATPIYEAQYRIR
                     HREGHWVWIRSRGRVVERDAGGRALRLVGTDADISRDKAAEEQLRHRAEFDLLTGLPN
                     RALFTDRLAGAIERTLRGGTALALLFVDVDRFKDVNDRHGHEAGDALLKIAAGRFQQS
                     VRSVDTVARLAGDEFTVILEGLADPADAEVVARKLIESMRVPMQVSGALLRVSVSIGI
                     AFLEPGETDPARLLRRADEALYDAKRRGRGRYALSPLPLAAD"
     gene            complement(2677..>2889)
                     /locus_tag="VFF72_08875"
     CDS             complement(2677..>2889)
                     /locus_tag="VFF72_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZV93317.1"
                     /translation="PMSTGRNFDEILRALDSIQLTAKHPVATPVNWKPGEDIVVSGSV
                     SDEDAKKKFPGFKTIKPYLRTAKQPS"
BASE COUNT          429 a         1037 c         1024 g          399 t
ORIGIN      
        1 tcgtactgaa ggaagacgtt ggctgcaccg atctcgtcga tcaacgagat cgcctgcgcc
       61 gagcgattca cgaagtagcc gggcgcgtcg aagcggttga gcggctcgac cagcacccgg
      121 acccgggcct cggcgaaccg cgcggcggcg aaggcgaggt tggcgacata ggtggcgcgc
      181 gctgcgtcgg cgccgacgcc atcggggacg atgccggcga ggacgtgcac gttgccgacg
      241 ccaagcgcct gcgcgtagtc gagggcgcgc aaggcgccgt cgcgaaactc cgcctcgcga
      301 cccgggatgc acgccatgcc gcgctcgccg cccgccgcat cgcccggggg cgtgttgaaa
      361 agcacggcgc gcaggccatg cgcctgcaat cgcgcccgga tgtccggggc cgggtgggcg
      421 taggggaaga ggaactcgac cgccgcgaag ccggcgcgcg ccgcatgctc gaaacgatcg
      481 aggaacggcc gctcggggaa gaggaaggag caattggcgg caaggcgagg catcgccgcg
      541 attgtgccgg gcggcgggaa ttgcgccacg aacgggcccc tcatcgagcc accgccggcg
      601 tgccggctgc ggcaagatcg cgggcgctcc ttcgcgcgct ccacgctgcc ccgtgccttc
      661 gtccgtcaag agaccgatcc gcctcggcat ccagccgcgc atgctgctgg tcgcgctggc
      721 catcggctcg ctgttcctcg cctacatcgc cgtcacgacc gtccgccagg ccagccgcga
      781 tcgcgcccag gcgggcgagg agatgcgcct ggtcgccgca ctggccggcg cgcgcctcga
      841 cgatcacatc ggcgacgcga cccagctgct gaatgcgctg gccggcaccc tgactgtcga
      901 cgtcgacgac accgccgtca acgacgccac cttggggtct cttgcgccgc aacttcccgc
      961 cagcgtcagc gacgtgtcgt tgtggacgat cgacggcacc aacatcggca gctcggagac
     1021 gagccccgcg atgcctcgcc gcaacgcgtc ggatcacgag ttcctcgccg cggcgctgcg
     1081 tgccgacggc gtcgcgatcg aggcgccgct gcgctggcgc aagggcggcg actggaccgc
     1141 ggtattcgcc catcgcgtgg ttcgcgacaa gaaggtcatc ggcgtcgtct cggtcgagac
     1201 gccgctgtcg gcactgccgc ggatgctcga tcccgagggc acgctgccgg ccggcgcggt
     1261 gatcaccctc gtggacgcgc aaggccgcat cgtcgctcgc tcgctcgacg ccgaacgctg
     1321 gatcggccag ccggcgccgc tcgacccggt cgagctgcag cagcgcatgg ccatgggccg
     1381 aggcagcgcc gaaaacgacg gcctcgacgg caagccgcgc gtcttcggct tcgcccgcgc
     1441 ccgcggcatg ccgtggctgg tctacgtcgg catgccggtc gacgtcgcgc tggcatcggc
     1501 caacggcaac acgctgcaaa gcctcgccat ggggctgggc atgctcgccg tcggcctgct
     1561 gctcgccgca tgggtcgcga gccgcatcac gcggccgctg cgccagctga gtgccgacgc
     1621 gcgcctgctc ggcgaaggac gattcgagca ccgcagcgcc gttcgcacgg gctcggagat
     1681 cggcctgctc gcgcacaccc tcaaccgcat ggccgaggcc ctgcaggaac ggatcgccgc
     1741 ggcccggcgc agcgccgaac ggcttggcct ggccctggaa gggtcgaacc aggcgctgtt
     1801 cgactgggac atcgccggcc ggcgcgtcca ctacagcgcc cgcgcgtcga tgcttcgagg
     1861 cggccccgac gaggagatcg acgcgtcgcc cgaagagatg cgcgccttcg tccacccgga
     1921 cgacctgccg accgtgctcg gccgcctgac cgacgcggtg cgcggggcga cgccgatcta
     1981 cgaggcgcaa tatcgaatca ggcatcgcga aggccactgg gtctggatcc gcagccgcgg
     2041 ccgcgtcgtc gaacgcgacg ccggcgggcg ggcgctcagg ctggtcggca ccgatgccga
     2101 catcagccgc gacaaggcgg ccgaggagca gttgcgccat cgcgccgaat tcgatctgct
     2161 gaccggcctg cccaatcgcg cgctcttcac cgaccggctg gccggcgcga tcgagcgcac
     2221 gcttcggggc ggcaccgcac tcgcgttgct gttcgtcgac gtcgaccgct tcaaggacgt
     2281 caacgaccgg cacggtcacg aagccggtga cgcgctgctg aagatcgccg ccggccggtt
     2341 tcagcaatcg gtgcgcagcg tcgacacggt cgcccggctc gccggcgacg aattcacggt
     2401 cattctcgaa ggcctggccg accccgccga cgccgaggtc gtcgccagga agctgatcga
     2461 atcgatgcgg gtgccgatgc aggtcagcgg ggcgctgctg cgcgtctcgg tgagcatcgg
     2521 catcgccttt ctcgagcccg gcgagaccga cccggcgcgg ttgctgcgcc gcgccgacga
     2581 ggcgctctac gacgccaagc ggcgcggtcg cggccgctac gcgctcagcc cgctgccgct
     2641 ggccgcggac tgagcacgcg gttcaggcga ggccggtcag ctcggctgct tggccgtgcg
     2701 caggtacggc ttgatggtct tgaagccggg gaatttcttc ttcgcgtcct cgtcggagac
     2761 cgagccggaa acgacgatgt cctcgcccgg cttccagttc accggcgtcg caaccgggtg
     2821 cttcgcggtc agctggatcg aatcgagcgc gcgcaggatc tcgtcgaagt tgcggccggt
     2881 gctcatcgg
//
LOCUS       DASRWS010000470         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3379447, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000470 DASRWS010000000
VERSION     DASRWS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379447"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VFF72_08880"
     CDS             <1..1424
                     /locus_tag="VFF72_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93318.1"
                     /translation="DGPYYFFKLIQRMRQLLPPWMPSVGLEGGRINIVPVDFVVAALD
                     HISHLKTDLDSRCFHLVDPVGYRVGDVLDIFSKAAHAPKMNLFVNAALLGFIPKSVKK
                     GLMAVAPVRRIKRAVLKDLGLPEDMLTFINFPTRYDCREALAALKGSGIECPRLPEYA
                     WKLWDYWERHLDPALTIDHSLRGTVAGKVVLVTGGSSGIGLAAACRFAEAGAITIICA
                     RDEAKLMEARKEILARAGKGAIVETYSADIADEASCKGLVDWLAATHGGVDFLVNNAG
                     RSIRRAIEASYERFHDFERTMQLNYFGCLRVTMGLLPGMVAKKKGHIVNISSIGVLTN
                     APRFSAYVASKAALDAWTRCASSEYADMGITFTTINMPLVRTPMIGPTRIYDNVPVLS
                     PEEAASMIAEACVEKPVRIATRLGITGELLHALVPRVAQIVMNSTFRMFPDSAAAKGD
                     KDKKYTPSPEAIAMQQMMRGIHF"
     gene            complement(1551..>2262)
                     /locus_tag="VFF72_08885"
     CDS             complement(1551..>2262)
                     /locus_tag="VFF72_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZV93319.1"
                     /translation="AQLKAAGITDLVLDLRYNGGGYLDIASELAYMIAGPTVTTSATP
                     TPAYFEREIFNDRNPFNVSTAQATTPFHDHGQNFTVSSSQALPVLGLSRVYVLTSPDT
                     CSASEAIINGLEGVGVTVELIGTTTCGKPYGFYPKDNCGTTYFAIQFQGVNNMGSGNY
                     ADGFAPTCTVDDDFAHALGDPAEAQLKEALALRDSGSCTPSGGNGNPHALAAPLRRST
                     GPVLVRNPFRENRILRAQ"
BASE COUNT          375 a          724 c          779 g          384 t
ORIGIN      
        1 tcgacggccc ctattacttc ttcaagctca tccagcggat gcgccagctg ctgccgccgt
       61 ggatgccctc ggtgggcctc gagggaggcc gcatcaacat cgtgccggtc gacttcgtgg
      121 tcgcggcgct cgatcacatc agccacctga agaccgacct cgacagtcga tgcttccacc
      181 tggtcgaccc ggtcggctat cgcgtcggcg acgtgctcga catcttcagc aaggcggcgc
      241 atgcgccgaa gatgaatctc ttcgtcaacg ccgcgctgct cggcttcatc ccgaagagcg
      301 tgaagaaagg cctgatggcg gtggcgccgg tgcgccggat caagcgcgcg gtgctgaagg
      361 acctgggcct gcccgaggac atgctcacct tcatcaattt tccgacgcgc tacgactgtc
      421 gcgaggcgct tgcggcgttg aagggctcgg gcatcgagtg tccgaggctg cccgagtacg
      481 cgtggaagct ctgggactac tgggagcgcc acctcgaccc ggcgctgacg attgatcact
      541 cgctgcgcgg cacggtggcg ggcaaggtgg tgctggtgac cggcggctcg tcaggcatcg
      601 gcctggcggc cgcatgcagg ttcgccgagg ccggtgccat caccatcatc tgcgcccgcg
      661 acgaagccaa gctcatggag gccaggaagg agatcctggc cagggcgggc aagggcgcga
      721 tcgtcgagac gtactcggcc gacatcgccg acgaagcgag ctgcaagggc cttgtcgact
      781 ggctcgcggc gacgcacggc ggcgtcgact tcctcgtcaa caacgccggc cggtcgatcc
      841 gccgtgccat cgaggcgagc tacgagcgct tccacgattt cgagcggacg atgcagctca
      901 actacttcgg ctgcctgcgc gtgacgatgg gcctcctgcc gggcatggtg gcgaagaaga
      961 agggtcacat cgtcaacatc agctcgatcg gcgtgctgac gaacgcgccg cgcttttcgg
     1021 cctacgtcgc gtcgaaggcg gcgctcgatg cctggacgcg ctgcgcctcg agcgagtacg
     1081 ccgacatggg catcaccttc acgacgatca acatgccgct ggtgcgcacg ccgatgatcg
     1141 gcccgaccag gatctacgac aacgtgccgg tgctctcgcc tgaagaagcg gccagcatga
     1201 tcgccgaagc ctgcgtcgaa aagccggtcc gcatcgcgac gcgcctgggg atcaccggcg
     1261 agttgctgca cgcgctggtg cctcgcgtgg cgcagatcgt catgaattcg acgttccgga
     1321 tgttccccga cagcgccgcc gccaagggcg acaaggacaa gaagtacacg ccgtcgccgg
     1381 aagcgatcgc gatgcagcag atgatgcgcg gcatccactt ctgattcgcg tggactgcgg
     1441 cgttgccgtc gtccaggcgg gttgcggtcg ttccatggca cgacgcgccg aaggcgcggc
     1501 gtttgccaac ccgaccgccg caagcgcgcg gtgcttggcg agatggcctg ctactgcgct
     1561 cgcaggatgc ggttttcccg gaacggattg cgcacgagga ccggaccggt cgaccgccgc
     1621 aatggcgcgg ccagcgcgtg cggattgccg ttgccgcccg aaggcgtgca gctgccgctg
     1681 tcgcgcagcg ccagggcttc cttcaactgc gcctcggcgg ggtcaccgag cgcgtgcgcg
     1741 aaatcgtcgt cgacggtgca ggtcggagcg aacccgtcgg cgtagtttcc cgagcccatg
     1801 ttgttgacgc cctggaactg gatcgcgaag taggtcgtgc cgcagttgtc cttcggatag
     1861 aagccgtacg gcttgccgca ggtggtcgtg ccgatcagtt cgaccgtcac gccgacgccc
     1921 tcgaggccgt tgatgatcgc ctcgctcgcc gagcaggtgt cggggctggt caatacatag
     1981 acccggctca ggccgagcac gggcagcgcc tggctcgagg agacggtgaa attctggcca
     2041 tgatcgtgga acggcgtcgt cgcctgcgcg gtcgacacgt tgaacggatt gcggtcgttg
     2101 aagatctcgc gctcgaagta ggccggcgtc ggcgtggccg aggtcgtcac ggtcggcccg
     2161 gcgatcatgt aggcgagctc ggaggcgatg tcgagatagc cgccgccgtt gtagcgcagg
     2221 tcgagcacga ggtcggtgat gcccgcggcc ttcaattgcg cg
//
LOCUS       DASRWS010000471         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4214128, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000471 DASRWS010000000
VERSION     DASRWS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214128"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VFF72_08890"
     CDS             <1..117
                     /locus_tag="VFF72_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HZV93320.1"
                     /translation="RFLDHVQAHEYVWVARRIDIARHWKALHPFMPSPGASR"
     gene            114..1982
                     /gene="uraD"
                     /locus_tag="VFF72_08895"
     CDS             114..1982
                     /gene="uraD"
                     /locus_tag="VFF72_08895"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HZV93321.1"
                     /translation="MNRSITLDELNGASVEAFIVLLEGIYEHSPWVAEWAEPRRPFAT
                     LAQLKRVLFETVAGADRDRRLALLRAHPPLAGRAGTAPLTAASSDEQRRAGLAHCSAA
                     ELDALRRLDAAYLEKFGFPFILAVRGPRGDGLAKAQIIATLERRLEHHPDFEFEEALR
                     NVHRIAELRLDDRFGFRPRLGEQAWDCAELLARHSDPGFAERGELTVTYLTAAHRACS
                     AQLVRWMGECGFDDVTIDAVGNVVGVYEAEAGTAGTTAGHEAAAGAAAAKRARSAARG
                     RRLLTGSHFDTVRNGGRYDGRLGILVPMICVREMSRAGKRLPFAIELVAFAEEEGQRY
                     KATFLGSGALLGQFDPRWLDQRDADGTTMREAMRAARLPATVEAIAALRRDPAGYLGF
                     VEVHIEQGPVLAELGLPLAVVTSINGGVRLAGEVRGMASHAGTTPMTRRHDAAAAVAE
                     LAVYLEKRAGSEPNLVGTMGMLEVPNGSTNVVPGRCRFTLDIRATTDAVRDDCVADVL
                     AELHAICERRGVEASVEETMRAAACASSPQGQARWERAVAAAGLPVHAMPSGAGHDAM
                     KLGEIMPQAMLFVRGENAGISHNPLESTTADDMQLAIEAFQRMLDDLAGTPSKAAP"
     gene            1979..3208
                     /locus_tag="VFF72_08900"
     CDS             1979..3208
                     /locus_tag="VFF72_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZV93322.1"
                     /translation="MNACEQLDAWIDAHFDEETAFLQALVRVPTDTPPGDNAPHAERT
                     AALLAAFGFAADRYPVPAETVRERGLASITNLVVRRRYGEGPTVALNAHGDVVAPGDG
                     WTHDPYGGEIVDGKLYGRASAVSKSDFATYTFAVRALESLGASLAGSVELHFTYDEEF
                     GGELGPGWLLRMGLVKPDLLLAAGFSYQVVTAHNGCLQLEVTVHGKMAHAAIPETGID
                     ALQGATAILRALYAQNEIHRTITSKVEGITHPYLNVGRIEGGTHTNVVPGKVVLKLDR
                     RMIPEEDPAQVEAHLRRVIEDAAGAVPGITVAIRRLLLARALKPLPGNAPLVEALQRH
                     GEAVFGVRIPVSGTPLYTDVRLYGEAGIPAAIYGAGPRTVFESNAKRADEHLELDDLR
                     GATKVVARTLLELLSPR"
     gene            complement(3275..4234)
                     /locus_tag="VFF72_08905"
     CDS             complement(3275..4234)
                     /locus_tag="VFF72_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZV93323.1"
                     /translation="MTTSISEAAVRVAVVQAAAVPFDAGACVDKAVRLIADAAARGAR
                     VVVFPEAFIGGYPKGLGYGLVVGARDPVGREEFRLSYEAAIEVPGPLTVPLGEAAAAH
                     GCHVVMGVIEREHGTCYCTVLFFGPDGRLLGKHRKLMPTALERMIWGFGDGSTLTAID
                     SPYGVIGSVICWENYMPMLRMAMYAKNVALYCAPTADDRETWLPSMRHVALEGRCFVL
                     TACQYLRRGDFPQTVRVSLGDSPESVLMRGGSAIVGPLGQVLAGPDFDGETILVADLD
                     LAEIGRAKFDFDVVGHYSRPDVFQLSVNEQPMQAVVARGGVPG"
     gene            complement(4284..4943)
                     /locus_tag="VFF72_08910"
     CDS             complement(4284..4943)
                     /locus_tag="VFF72_08910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV93324.1"
                     /translation="MSATTGAPDMKDYYAARAAEYDAIYRKPERQADLRAIEAWLPPI
                     FAGARVLEVACGTGYWTQFIAPVASALRAIDAAPETMRIAAARVDASRVRFEVGDAYA
                     LRPSDPACDAAFAGFWFSHVPKARRLEFLAGLQAVLVPGSTVVLLDNRYVEGSSTTVS
                     AADPAGDTWQVRTLADGSVHRVLKNFPGEAELRSLVEGIGTDVVHTSWPHYWALRYRT
                     R"
     gene            complement(4940..5149)
                     /locus_tag="VFF72_08915"
     CDS             complement(4940..5149)
                     /locus_tag="VFF72_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93325.1"
                     /translation="MNLPDIEIVSAEVHAAWLESKRRQGVTSRRGEDGEELMVAYEQL
                     SEKAKELDRVTVRAVYAAIRRAGGT"
BASE COUNT          796 a         1973 c         1865 g          749 t
ORIGIN      
        1 cgcttcctcg accacgtcca ggcccacgag tacgtctggg tggcgcgacg catcgacatc
       61 gcacggcact ggaaggcact tcatccattc atgccgtcgc cgggagcttc gcgatgaacc
      121 gcagcatcac gctcgatgaa ctgaacggcg cctcggtcga ggccttcatc gtcctgctcg
      181 agggcatcta cgagcattcg ccctgggtcg ccgaatgggc cgagccgcga cggccgttcg
      241 cgacgctggc gcagctcaag cgcgtcctct tcgagaccgt cgccggcgcc gaccgcgatc
      301 gccgcctcgc cctgctgcgc gcgcatccgc cgctggccgg tcgtgccggc accgcgcctc
      361 tcacggcagc gtcgtccgac gaacaacggc gcgccggcct ggcccattgc agcgccgccg
      421 agctcgacgc gctgcgccgt ctcgacgccg cctatctcga gaaattcggc tttccgttca
      481 tcctcgccgt tcgcggaccg cgcggcgacg gcctcgccaa ggcgcagatc atcgccacgc
      541 tcgagcgtcg actcgagcac caccccgact tcgagttcga ggaagcgctg cgcaacgtgc
      601 accgcatcgc cgagctgcgt ctcgacgacc gcttcggctt ccggccgcgc ctgggcgagc
      661 aggcatggga ctgcgccgag ctgctggccc gccattccga ccccggcttc gccgagcgcg
      721 gcgagctcac cgtcacctac cttaccgccg cgcatcgcgc ctgctcggcg cagctcgtgc
      781 gctggatggg cgaatgcggt ttcgacgacg tcaccatcga cgccgtcggc aacgtcgtcg
      841 gcgtctacga ggccgaagcc ggcacggccg gaacgacggc cgggcacgaa gcggccgccg
      901 gggccgccgc cgcgaagcgg gcgaggagcg cggcgcgagg acgccgcctt ctcaccggca
      961 gccatttcga caccgtgcgc aacggcggcc gctacgacgg ccggctcggc atcctggtgc
     1021 cgatgatctg cgtgcgcgaa atgagccgcg ccggcaagcg cctgcccttc gcgatcgagc
     1081 tcgtcgcgtt cgccgaggag gagggccagc gctacaaggc gaccttcctc ggctcgggcg
     1141 cgctgctcgg ccagttcgat ccgcgctggc tcgatcagcg cgacgccgac ggaacgacga
     1201 tgcgcgaagc gatgcgcgcc gcccgcctgc cggcgacggt cgaggccatc gccgcgctca
     1261 ggcgcgaccc ggccggctac ctcggcttcg tcgaggtgca catcgagcag ggtccggtgc
     1321 tcgccgagct cggcctgccg ctggccgtgg tcacgtcgat caacggcggc gtgcgactgg
     1381 cgggcgaggt ccgcggcatg gccagccacg ccggcacgac gccgatgacg cgccggcacg
     1441 acgcggccgc ggccgtcgcc gagctcgcgg tctatctcga aaagcgcgcc ggctccgagc
     1501 cgaatctcgt cggcaccatg ggcatgctcg aggtgccgaa cggctcgacc aacgtcgtgc
     1561 ccggccgctg ccgcttcacc ctcgacatcc gggccacgac ggacgcggtc cgcgacgact
     1621 gcgtcgccga cgtgctggcc gagctgcacg cgatctgcga gcgtcgcggc gtcgaggcgt
     1681 cggtggagga gacgatgcgg gcagcggcgt gcgcgtcgtc tccgcaaggg caggcacgct
     1741 gggagcgcgc ggtcgccgcc gccggcctgc cggtgcacgc gatgccgagc ggcgccggcc
     1801 acgacgccat gaagctcggc gagatcatgc cgcaggcgat gctcttcgtg cgcggcgaaa
     1861 acgccgggat cagccacaac ccgctcgaat cgaccaccgc cgacgacatg cagctggcga
     1921 tcgaggcgtt ccagcgcatg ctcgacgatc tcgccggcac gccgtcgaaa gccgctccat
     1981 gaacgcctgc gagcagctcg acgcctggat cgacgcccac ttcgacgagg agacggcgtt
     2041 cctgcaggcc ctggtgcgtg tgcccaccga cacgccgccg ggcgacaacg cgccgcacgc
     2101 cgagcggacc gcggcgctgc tcgcggcctt cggcttcgcc gccgaccgct accccgttcc
     2161 ggccgaaacc gtgcgcgaac gcggcctcgc ctcgatcacc aacctcgtcg tccgtcgccg
     2221 ttacggggag ggcccgaccg tcgccctcaa cgcgcacggc gacgtcgtcg cgccgggcga
     2281 cggctggacc cacgacccct acggcggcga gatcgtcgac ggcaagctct acggccgggc
     2341 ctcggccgtc agcaagagcg atttcgcgac ctacacgttc gccgtgcgcg ccctcgagtc
     2401 gctcggcgcg tcgctcgccg gcagcgtgga attgcacttc acctacgacg aggagttcgg
     2461 cggcgagctc ggccccggct ggctgctgcg aatgggcctc gtcaagcccg acctgctgct
     2521 cgccgccggc ttcagctacc aggtcgtcac cgcgcacaac ggctgcctgc agctcgaggt
     2581 gaccgtccac ggcaagatgg cgcacgccgc gattcccgag accggcatcg acgcgctgca
     2641 gggcgcgacc gcgatcctgc gggcgctcta cgcgcaaaac gagatccacc gcacgatcac
     2701 gtcgaaggtc gagggcatca cgcacccgta cctcaacgtc ggccgcatcg agggcggcac
     2761 gcacaccaac gtcgttccgg gcaaggtcgt gctcaagctc gaccggcgca tgatccccga
     2821 ggaagatccg gcgcaggtcg aggcgcatct gcgtcgcgtc atcgaggacg ccgccggcgc
     2881 cgtgccgggg atcaccgtcg cgatccggcg gctccttctg gcgcgggcct tgaagccgct
     2941 gcccggcaac gcgcccctgg tcgaggcgct gcagcgtcac ggcgaggccg tcttcggcgt
     3001 gcggattccg gtctcgggaa cgccgctcta taccgacgtg cgcctctacg gcgaggcggg
     3061 catcccggca gcgatctatg gtgccgggcc tcgcaccgtg ttcgagtcga atgccaagcg
     3121 cgccgacgag catctcgagc tcgacgacct gcgcggtgcg acgaaggtcg tggcgagaac
     3181 gctgctcgag ctgctgtcgc cgcgttagcc gggcgcgggc ccgagacgac gttgcgaagt
     3241 tcggtcgggc gcgccgcgga cggtgtcgcg agagtcagcc cggcacgccg cccctggcga
     3301 cgaccgcctg catcggctgc tcgttgacgc tcagctggaa gacgtcggga cggctgtagt
     3361 ggccgacgac gtcgaagtcg aacttcgcgc ggccgatctc ggccagatcg aggtcggcga
     3421 cgaggatcgt ctcgccgtcg aaatcggggc cggcgagcac ctggccgagc gggccgacga
     3481 tggcgctgcc gccgcgcatc agcaccgact cgggcgaatc gccgagcgag acgcgcaccg
     3541 tctgcgggaa gtcgccgcgg cgcaggtact ggcaggcggt gaggacgaag cagcgcccct
     3601 cgagagcgac atggcgcatc gacggcaacc acgtctcgcg atcgtcggcg gtcggcgcgc
     3661 aatagagggc gacattcttc gcgtacatcg ccatgcgcag catcggcatg tagttttccc
     3721 agcagatcac cgagccgatg acgccgtacg ggctgtcgat cgcggtcagc gtcgagccgt
     3781 cgccgaagcc ccagatcatg cgctcgagcg cggtcggcat gagcttcctg tgcttgccga
     3841 gaagccgccc gtcggggccg aagaaaagca cggtgcagta gcacgtgccg tgctcgcgct
     3901 cgatcacgcc catgacgacg tggcagccgt gcgcggcggc ggcctcgccg agcggcaccg
     3961 tgagcggccc cggcacctcg atcgccgctt cgtacgagag ccggaattcc tcgcggccga
     4021 ccgggtcgcg tgcgccgacg accaggccgt agcccaggcc cttcgggtag ccgccgatga
     4081 aggcctcggg aaagacgacg accctggcgc ctcgcgccgc agcgtcggcg atcagccgca
     4141 ccgccttgtc gacacatgcg ccggcgtcga acggcactgc cgcggcctgt accacggcga
     4201 cgcgaaccgc cgcctcgctg atcgatgtcg tcatgcttcg tccttcctgc cggtgatggg
     4261 gttctcgccg ccgcccgcgg cattcatcga gtgcgatagc gcagcgccca gtagtgcggc
     4321 cagctggtgt ggacgacgtc ggtgccgatg ccctcgacga gcgagcgcaa ctccgcttcg
     4381 cccgggaagt tcttcagcac gcgatgcacg ctgccgtcgg cgagcgtgcg aacctgccac
     4441 gtatcgcccg ccgggtcggc ggcgctcacc gtcgtgctgc tgccttcgac gtagcggttg
     4501 tcgagcagca cgacggtcga tccgggaacc agcacggcct gcagcccggc gaggaactcg
     4561 aggcgacgcg ccttcggcac gtgcgagaac cagaagccgg cgaaggccgc gtcgcacgcg
     4621 ggatcgctcg gccgcaacgc gtaggcgtcg ccgacctcga agcgcacccg gctggcgtcg
     4681 acccgcgccg cggcaatgcg catcgtctcg ggcgcggcgt cgatcgcccg caaggcgctg
     4741 gcgacgggag cgatgaactg cgtccagtaa ccggtgccgc aagcgacttc gaggacgcgg
     4801 gcgcccgcga agatcggcgg caaccatgcc tcgatcgcgc gcaggtcggc ctgccgctcg
     4861 ggctttcgat agatcgcgtc gtactccgcg gcgcgcgcgg cgtagtagtc cttcatgtcc
     4921 ggcgcgccgg tcgtcgcgct catgtgccgc cggcgcgccg gatcgcggcg tagacggccc
     4981 ggacggtgac gcggtcgagc tccttcgcct tctcgctcag ctgctcgtac gccaccatga
     5041 gctcctcgcc gtcctcgccg cggcgcgagg tcacgccctg gcgacgcttc gattcgagcc
     5101 aggcggcgtg gacttcggcg gagacgatct cgatgtcggg caggttcatg gtcacgttcc
     5161 caggcgaagc gatgcaggtg cttcgaagcc ggcgcggcct gcggcggatg gtgttcgatc
     5221 cggtggcgac cgccccggcg cggacacgaa cgcggcgcgg cgcctcacgc cgcaagcccc
     5281 gtggccacga cgtcgccgag ccgggccaga agacccgcct tcacgcgcgg ccactcgtcg
     5341 tcgatgatgc tgaagcggac cgagttgcgc ttgcggccgt cgg
//
LOCUS       DASRWS010000472         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1347527, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000472 DASRWS010000000
VERSION     DASRWS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347527"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VFF72_08920"
     CDS             complement(<1..1019)
                     /locus_tag="VFF72_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HZV93326.1"
                     /translation="MDRLSHPELHASRLWSLPIEGMSCASCASRVESALMAVPGVREA
                     AVNLATEKAGVRADATVDASALLAAVERAGYEVPARRLALQIEGMTCSSCAGRVEKAL
                     LAVPGVVSAEVNLATERVALGLLGAVADRDVTAAVERAGYAATIVPRAPGAGESTMAP
                     RASGWPIAVAAVLSLPLIVPMLGAPFGRHWMLDGWVQLALATPVQFWLGARFYRAVWK
                     ALRAGTGNMDLLVALGTSAAYGLSLWQLFASRGDATHLYFEASAVVITLVLLGKWLEA
                     RAKNQTTEAIRALNALRPETARVRRDRVEVEVALADVAVGDLVVVRPGERIAVDGTVA
                     EGSSQV"
     gene            1178..1369
                     /locus_tag="VFF72_08925"
     CDS             1178..1369
                     /locus_tag="VFF72_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZV93327.1"
                     /translation="MISFDIPSMSCDHCAGVITRSVKAADPAAEVVVDLANHHVRIET
                     TQSREAIAATLAKAGYAPA"
     gene            1558..2349
                     /locus_tag="VFF72_08930"
     CDS             1558..2349
                     /locus_tag="VFF72_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93328.1"
                     /translation="MNNKNDTPAGQALSKVPEVVFGFWVIKIAATTLGETGGDWVSMS
                     LNLGYLVGSAIFAVIFVLLVSGQVKAERFRPVLYWATIVATTTLGTTLADFADRSVGL
                     GYPGGVTIVATLLAASLAIWYWSEGSVSVRSITRPKVEWFYWVTILFSQTLGTALGDW
                     MAGSDRGGLGLGYEIGALVFGAGLVVAAALYFWTRVSRTLLFWLAFVLTRPLGATLGD
                     LLDKPLAAGGLNLSRLYASLVLLAFIALCIHFIPQRAARAGESTA"
     gene            2370..>2797
                     /locus_tag="VFF72_08935"
     CDS             2370..>2797
                     /locus_tag="VFF72_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93329.1"
                     /translation="MPRLARWRLPAALATACACLLGCATPATGQHTVVERFLVGGEGG
                     WDFLTFDAGGNRLFIARANVVQVWSPRSQRLVGEIADTPGVHGIAVAPELHRGFTSNG
                     HADTVTVFGLGDLRRIATIPVSGRNPDAILYDPSFRRVYT"
BASE COUNT          435 a         1008 c          910 g          444 t
ORIGIN      
        1 acctggctcg aaccttcggc gaccgtcccg tccacggcga tgcgctcgcc gggccggacc
       61 acgacgagat cgcccaccgc gacgtccgcc agtgccacct cgacctcgac ccgatcgcga
      121 cgcacgcgtg ccgtctccgg acgcagtgcg ttgagggcac gaatcgcttc cgtcgtctga
      181 ttcttggccc gcgcctccag ccacttgccg aggagaacca gggtgatgac cacggccgag
      241 gcctcgaagt acaggtgcgt cgcgtcgccc cggctcgcga aaagctgcca gagactcagc
      301 ccgtaggcgg ccgaagtgcc cagcgccacc agcagatcca tgttgccggt accagcgcgc
      361 agcgccttcc agacggcgcg atagaagcga gcgccgagcc agaactgcac cggcgtcgcc
      421 aacgccagct gcacccagcc gtcgagcatc cagtgccgcc cgaaaggtgc gccgagcatc
      481 ggcacgatca gcggcaatga aagcaccgca gcgacggcga tcggccaccc gctcgctcgg
      541 ggggccatcg tcgactcccc ggcaccagga gcgcgcggca cgatcgtcgc cgcgtagccg
      601 gccctttcca ctgccgccgt cacgtcccgg tcggcgaccg caccgagcag gccgagcgcc
      661 acccgttcgg tcgccagatt cacttcggcc gacacgacgc cgggcaccgc cagcagggcc
      721 ttctccacgc ggcccgcgca cgacgagcac gtcatccctt cgatctgcag cgcgaggcga
      781 cgcgccggga cttcgtagcc ggcccgctcg acggcggcaa gcaaggcgga agcgtcgacc
      841 gtcgcgtcgg cgcgcacgcc ggccttctcg gtggcgaggt tgacggcagc ctcccgaacg
      901 cccggaaccg ccatcaaggc gctctccacg cgcgaggcgc acgaagcgca gctcattccc
      961 tcgatcggca gcgaccacag ccgggaggcg tggagttcgg ggtggctcag gcgatccatg
     1021 gtcggcatcc ttcagaaggc catgccagtg tcaaccctcc cacgatgtca aggtcaagcg
     1081 gtttcgaatt ccctttcccg cgcagggtcc gggccttgac cttcccatcg ccggaagccc
     1141 gacacccgag gcctcgttgc cgattgggaa ggtggccatg atcagtttcg acatcccttc
     1201 gatgagttgc gatcactgcg caggcgtgat cacgcggagc gtcaaggctg ccgacccggc
     1261 tgccgaagtc gtggtcgacc tcgcgaacca ccatgtgcgc atcgagacga cgcaatcgcg
     1321 agaggcgatc gccgcgacac tggcgaaggc aggctacgcg ccggcctgac gcttcggctc
     1381 gcagcgactt cgaggcgcgc ggtcacgacg agccgatcgg cgggacgtcc ggtcgaacat
     1441 gccccgggga tacgcctggc gtcttaaggc cgccttaaag gcgcactgcg gtcaacgggt
     1501 tgcgggaacg ccgcccgcag cgacgccgcg cgcgtgtgac actgtcggcc acgaatcatg
     1561 aacaacaaga acgacacccc ggccgggcag gctttgagca aggttcctga agtcgtcttt
     1621 ggcttctggg tcatcaagat cgctgcgacc accctcggcg agaccggtgg cgactgggtg
     1681 agcatgtcgc tcaatctcgg atacctcgtc ggctcggcga tcttcgcggt gatcttcgtc
     1741 ctcctggtgt cgggccaggt gaaggccgaa cgcttccggc cggtgctcta ctgggcaacc
     1801 atcgtggcca cgaccacgct gggcaccacc ctggccgact ttgccgaccg gtcggtgggc
     1861 ctcggctatc ccggcggcgt gaccatcgtc gccaccttgc tcgcggcgag cctggcgatc
     1921 tggtactgga gcgagggctc ggtctcggtg cgaagcatca cccggccgaa ggtcgagtgg
     1981 ttctactggg tgacgatcct cttctctcag actctcggca ccgcgctcgg cgactggatg
     2041 gcggggtctg atcgaggcgg gctcgggctg ggctacgaga tcggagcgct ggtcttcggc
     2101 gccggcctcg tggtcgcggc cgcgctgtac ttctggacgc gcgtgtcgcg aaccctgctc
     2161 ttctggctcg cgttcgtgct caccaggccg ctcggcgcga cgctgggcga tctgctcgac
     2221 aagcccctgg ccgcgggcgg cctcaacctg agccgcctct acgcctcgct ggtcctcctg
     2281 gccttcatcg ccctttgcat ccatttcatt ccgcagcgcg cggcgcgcgc cggcgagtcg
     2341 acggcgtgaa ccatcggcgc gatgctgcca tgccgcgact cgcgcgctgg cgactccccg
     2401 ccgccctggc gacggcgtgc gcatgcctgc tcggctgtgc cactccggcg accggacagc
     2461 acactgtcgt cgagcgcttt ctcgtcggcg gtgaaggagg ctgggatttc ctgacattcg
     2521 acgcgggcgg caaccgtctt ttcatcgcgc gcgcaaacgt cgtccaggtc tggagcccgc
     2581 gcagccagcg gctggtgggc gagattgccg atacccccgg ggtgcacggc atcgccgtgg
     2641 cgcctgaact gcaccgcggc ttcacctcca acggccacgc cgacacggtg accgtcttcg
     2701 ggctgggcga cttgcgcagg atcgcgacga tccccgtcag cggccgcaat cccgacgcga
     2761 tcctctatga cccgtcgttc aggcgcgtct acacctt
//
LOCUS       DASRWS010000473         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1347878, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000473 DASRWS010000000
VERSION     DASRWS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347878"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFF72_08940"
     CDS             complement(<1..724)
                     /locus_tag="VFF72_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZV93330.1"
                     /translation="METADSPSENASPAAASGLDFAVVGIGASAGGLAAVRALLEGLP
                     AAPDMAFVVVLHLSQHHESHAAALFQASTSMPVAQVQDPVRIERDHVYVAPPGHDLTA
                     VDGTLVPVECERSMGRHVVIDRFFRALADAYRQRAIGIVLSGAGADGAIGIGRLKEEG
                     GIVIAQAPDDAEYDGMPKNAIATGKVDLVLPVADIAKRLVEIRSNAARIALPGGSEVA
                     AGVREPAEPQAAEEALRDVMKTL"
     gene            839..2035
                     /locus_tag="VFF72_08945"
     CDS             839..2035
                     /locus_tag="VFF72_08945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV93331.1"
                     /translation="MTGPPLRSADGLGPHVVDATMFWSPTGGGVRRYLLAKDEGLLRR
                     GWRHTIAAPGLVPEAGRAPLPGLPLPASGGYRLPLRRGAVARVLEGLGPDLIEAGDPY
                     RVAWAALDAAQALGIPAVAYCHSNLEQMARLHTGRRFGDAAARLARRYTKHVYQHFAL
                     VLAPSLVMRGHLVDAGVERVACQPLGVDTRAFHPSRASSSWRVENGLAADARVLVYAG
                     RFAPEKHLDVLADAVTRLGAPYVLVAIGAGPTPPAASARVRVLPFVAGVPALATALAS
                     ADAFVHAGDQETFGLSALEAMACATPVVVRAAEGLAELVDGGGGIGVDRGDAASFAAA
                     IEALFATDRVQLRGAARRRAEASDWDRVLPGLLAHYLRLLGRVATTGAADAAPSRPAA
                     PLPVER"
     gene            2032..2859
                     /locus_tag="VFF72_08950"
     CDS             2032..2859
                     /locus_tag="VFF72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZV93332.1"
                     /translation="MSPRSPAATRPAGAADRFVCVVLHDVATSTRSACLRTLAAVAEV
                     ADVPLTLLAVPHYHGEASTRELEAWLTARSQRGDELALHGYTHRDDLPPRGWLDRLRR
                     RHYTRGEGEFWALTEVDARQRIETGIAWFRANGWPLHGFVAPAWLLGTQAWGALDGFE
                     YTSTLRQIVMLPGRERLTSQSVVYSTSSAWRRRTSLVWNAWVARRERANALLRIELHP
                     RDADFAAVRRSWQSILEHALRDRQPVTVADLVAHWRPNGEPRAAGTTTWQTTTARED"
     gene            complement(2889..4073)
                     /locus_tag="VFF72_08955"
     CDS             complement(2889..4073)
                     /locus_tag="VFF72_08955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZV93333.1"
                     /translation="MNDSARTMRIAYVTETWPPELNGVSLTVERTVRFLRSRGHRVEL
                     IRPRQPGEAALDASDELRTAGCAIPMYREMRFGLARVATLVRRFEQTRPEVVHLATPG
                     PLAWAALAAAGTLGIATTSDFRTNFHQYSRYYGLGSLAGVALGLLRRFHNLTRRTFVP
                     TRSARRELAAAEFHNLTIVGRGVDTSRFTPSRRSAALRESWGVGGDDVPVLLYVGRVA
                     AEKNVELALRAFAAIRQSRPDAQLVIVGDGPARRRLQALHPEARFVGVQRGDDLATHY
                     ASADIFLFPSLSDTFGNVVLEALAAGLPVVSFDCAAAAEHVDDGISGRLAVPGDEAAF
                     IAAAVELATAPDGVRAPMRSAAAAAAARAGWDEVLTRFESRLQDTVDATESAPARVPV
                     VA"
     gene            complement(4182..>4903)
                     /locus_tag="VFF72_08960"
     CDS             complement(4182..>4903)
                     /locus_tag="VFF72_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZV93334.1"
                     /translation="PYSTPVPLQRAPVLGDGALGARLASEGWTFTRLAIGETVSFALS
                     ASATVILLYFLLASEQWLVSRTAAAIPRRRTRAVVLSGFRQAQRDIGLFISTMSIVNL
                     ALGVATGLALWLIGLPNPVLWGTTTAVLAFIPYLGPLLITVLLLLAGSVTFGSGVTML
                     GPPAAFLLLHGIEANFLSPMIMGHRLRLSPVFVFLSVMVWGWLWGLAGAFIAVPLLLA
                     LRVVCKRVRRLHLLCAYLEGR"
BASE COUNT          701 a         1786 c         1767 g          649 t
ORIGIN      
        1 gcagcgtctt catgacgtcg cgcagcgcct cctcggccgc ctgcggctcg gccggctccc
       61 gaacgccggc ggcgacctcg ctgccgccgg gcaaggcgat gcgcgcggca ttgctgcgga
      121 tctcgaccag ccgcttcgcg atgtcggcca ccggcagcac gaggtcgacc ttgccggtgg
      181 caatcgcgtt tttcggcatg ccgtcgtatt cggcgtcgtc gggagcctgg gcgatgacga
      241 tgccgccttc ttccttcaga cggccgatgc cgatcgcgcc gtcggcaccg gcgcccgaaa
      301 gcacgatgcc gatcgcgcgc tgccggtagg cgtcggccag ggcgcgaaag aaccggtcga
      361 tgacgacatg gcggcccatc gaccgctcgc attcgaccgg caccagcgtg ccatcgaccg
      421 ccgtcaggtc gtggccggga ggggcgacgt agacatggtc gcgctcgatc cgcaccgggt
      481 cctgcacctg cgccaccggc atggatgtcg acgcctggaa gagcgccgcg gcgtggctct
      541 cgtggtgctg cgacagatgc aggacgacga cgaacgccat gtcgggcgcc gccggcaagc
      601 cctcgagcag ggcgcggacc gccgccagtc cgccggccga cgcgccgatg ccgacgacgg
      661 cgaagtcgag gccgctcgca gcggccggcg acgcgttctc gctgggtgag tctgccgtct
      721 ccatcgcgaa tctcaggata cgccgtcatt caggcgccgt gatggaagcg gatcgcgcac
      781 cggtcattcg cgatgccttc cggcacgacg ggcagcacct cgacggcaca atgtgccgat
      841 gaccgggccg ccccttcgca gcgccgacgg cctcggcccg cacgtcgtcg atgcgacgat
      901 gttctggagc ccgaccggcg gcggcgtgcg ccgctatctg ctggccaagg acgaaggcct
      961 gctgcggcgc ggctggcggc acaccatcgc cgcacccggc ctcgtgcccg aggcaggtcg
     1021 cgcgccgctg ccgggcctgc cgttgccggc gagcggcggc taccggttgc cgctgcgccg
     1081 cggcgcggtc gcccgcgtgc tcgaagggct cgggcccgac ctgatcgaag ccggcgatcc
     1141 gtatcgcgtc gcctgggccg cgctcgacgc ggcgcaagcg ttgggcattc ccgccgtcgc
     1201 ctactgccac agcaacctcg agcagatggc gcggctgcac accgggcgcc ggttcggcga
     1261 tgccgcggcg cggctcgcgc gccgctacac gaagcatgtc taccagcact tcgcgctggt
     1321 tctcgcgccc agcctcgtca tgcgcgggca tctcgtcgac gcgggcgtcg agcgcgtcgc
     1381 ctgccagccg ctcggcgtcg acacccgggc gtttcatccg tcgcgggcgt cgagcagctg
     1441 gcgtgtcgag aacggcctcg ccgcggatgc gcgcgtcctc gtctatgcgg gccgcttcgc
     1501 gcctgaaaag cacctcgatg tgctcgccga tgcagtgact cgtctcggcg cgccctacgt
     1561 gctcgttgcc atcggcgccg gcccgacgcc accggccgcc tcggcgcgcg tgcgcgtgct
     1621 gccgttcgtc gccggcgtgc cggcgctggc caccgcgctg gccagcgccg acgccttcgt
     1681 ccacgccggc gaccaggaaa ccttcggcct gtcggcgctc gaagcgatgg cctgcgccac
     1741 gccggtcgtc gtgcgcgccg ccgaagggct cgccgagctg gtcgacggcg gcggcggcat
     1801 cggcgtcgac cggggcgacg ccgcctcctt cgcagcggcg atcgaggcgc tcttcgcgac
     1861 cgatcgcgtc cagcttcgcg gcgccgcccg ccgccgcgcc gaagcgagcg actgggaccg
     1921 cgtcctgccc ggccttctcg cgcattacct ccggctgctc ggccgtgtgg cgacgacggg
     1981 cgctgccgac gccgcgccgt cgcgccccgc tgcgccgttg cccgtcgagc gatgagcccg
     2041 cgcagccccg cggcaacgcg tccggccggc gccgccgacc gcttcgtctg cgtcgtcctg
     2101 cacgacgtcg cgacgtcgac ccggtcggcg tgcctgcgca cgctggccgc cgtcgccgaa
     2161 gtcgccgacg tgccgctgac gcttcttgcg gtgccccact atcacggcga ggcgtcgacg
     2221 cgcgagctcg aggcgtggct caccgcacgc tcgcagcgcg gcgacgagct cgcgctgcac
     2281 ggctacacgc atcgcgacga cctgccgccg cggggctggc tcgaccgcct ccggcgccga
     2341 cactacacgc gcggcgaggg ggagttctgg gccctcaccg aagtcgacgc tcgacagcgc
     2401 atcgaaaccg gcatcgcctg gtttcgcgcc aacggctggc cgctgcacgg tttcgtcgcg
     2461 cccgcgtggc tgctcgggac gcaggcctgg ggcgccctcg acggcttcga gtacacgtcg
     2521 acactgcgcc agatcgtcat gctgccgggc cgcgagcggc tgacgagcca gagcgtcgtc
     2581 tacagcacct cgagcgcctg gcggcggcgc acctcgctgg tctggaacgc ctgggtcgcg
     2641 cggcgcgagc gcgccaacgc gctgctgcgc atcgagcttc atccacgcga cgccgatttc
     2701 gccgcggtgc gccgctcatg gcagtcgatc ctcgaacacg cgttgcgcga tcgacagccc
     2761 gtgacggtgg ccgacctcgt cgcgcactgg cggccgaacg gtgagccgcg tgctgctggc
     2821 accaccacgt ggcagacgac cacggccagg gaagactgaa gtcgagcgcc gccgcgcgac
     2881 ggcgtcgatc aggccacgac agggacgcgg gcgggcgccg attcggtggc gtcgacggtg
     2941 tcctgcaggc gcgactcgaa tcgtgtcagc acctcgtccc agccggcacg cgccgcggcg
     3001 gcggcggcgg ctgaacgcat cggcgcgcgc acgccgtccg gggccgtggc aagctcgacc
     3061 gccgcggcga tgaacgccgc ttcgtcgccg ggcaccgcca ggcggccgct gatgccgtcg
     3121 tcgacgtgct cggccgcggc agcgcaatcg aacgagacga cgggcagccc tgccgcgagc
     3181 gcctcgagga cgacgttgcc gaaggtgtcc gacaggctcg gaaaaagaaa gatgtcggcc
     3241 gacgcgtaat gcgtggcgag gtcgtcgccg cgctgcacgc cgacgaaacg ggcctcggga
     3301 tgaagcgcct gcaggcgccg ccgcgccggg ccgtcgccga cgatcacgag ttgcgcgtcg
     3361 ggtcgcgact ggcggatcgc cgcgaacgcg cgcaacgcga gctcgacgtt cttttccgcg
     3421 gcgacgcgac ccacgtagag cagcaccggc acgtcgtcgc cgcccacacc ccagctttcg
     3481 cgcagcgccg cgctgcgccg cgacggcgtg aagcgcgagg tgtcgacacc gcggccgacg
     3541 atcgtcaggt tgtggaactc cgccgccgcc agctcgcggc gcgccgagcg cgtcggcacg
     3601 aaggttcggc gcgtcaggtt gtggaagcgc cgcagcaggc cgagggccac gccggccagc
     3661 gagccgagcc cgtaatagcg gctgtactgg tggaagttgg tgcggaagtc ggaggtggtg
     3721 gcaatgccca gggtgccggc ggcggccagc gccgcccagg ccagcggccc tggcgtggcg
     3781 aggtgaacga cctcgggccg ggtctgctcg aaccggcgca ccagcgtggc gacgcgcgcg
     3841 agtccgaatc gcatctcgcg atacatcggg atcgcgcagc cggcggtgcg caactcgtcg
     3901 ctggcgtcga gcgccgcctc gccgggctgg cggggccgga tcagctcgac gcggtgaccg
     3961 cgcgagcgaa ggaagcggac cgtgcgctcg accgtcagcg agacgccatt gagctcgggc
     4021 ggccaggtct cggtcacgta ggcgatgcgc atcgtccggg ccgaatcgtt cacggcgtcg
     4081 gattggcaat ctccatgccc gtggctcatc gtcgctgtcg gatcggtcat gggaaaaagg
     4141 gttcctcgca gggtctgtcg gtggcctcgt cgaggtctcc ttcagcgccc ttccagatag
     4201 gcgcagagca gatgcaggcg tcgcacccgc ttgcagacga cgcgaagcgc gagcagcagc
     4261 ggcaccgcga tgaaagcgcc ggcgaggccc cagagccagc cccagaccat caccgacagg
     4321 aacacgaaga cggggctcag gcgaagccga tggcccatga tcatcgggct gaggaaattg
     4381 gcctcgatgc cgtgcaggag caggaacgcg gccggtggcc cgagcatcgt cacgccgctg
     4441 ccgaaggtga cgctgccggc aagcagcagc agcaccgtga tgagcagcgg cccgaggtag
     4501 ggaatgaagg cgagcacggc cgtcgtcgtt ccccacagca cggggttcgg caggccgatc
     4561 agccagagcg ccagacccgt cgccacgccg agagcgaggt tgacgatgct catcgtgctg
     4621 atgaagaggc cgatgtcgcg ctgcgcctgg cgaaatcccg acagcaccac cgcacgggtg
     4681 cggcggcggg gtatcgccgc cgcggtccgc gacaccagcc actgctcgga ggcgagcagg
     4741 aaatagagca ggatcaccgt cgccgatgcc gacagcgcga acgagaccgt ctcgccgatc
     4801 gccaggcgcg tgaaggtcca gccctcgctg gccagccggg cgccgagcgc gccgtcgccg
     4861 agcaccggcg cgcgctgcag cggcaccggc gtcgaatacg gaa
//
LOCUS       DASRWS010000474         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2772652, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000474 DASRWS010000000
VERSION     DASRWS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772652"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFF72_08965"
     CDS             <1..183
                     /locus_tag="VFF72_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93335.1"
                     /translation="EFTMRLDDYRALGGHVGRVRRLEDVLATTAHRVVAQVDGNPWPL
                     SADLAPASGAIAGRPS"
     gene            180..1139
                     /locus_tag="VFF72_08970"
     CDS             180..1139
                     /locus_tag="VFF72_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HZV93336.1"
                     /translation="MSTATRRPLGDGRWLFQHGPIDCVILAEGRPEVVAACRERAWQR
                     FRGVLDELVGELPLLRTDLGSAAGACVAPAGSIARRMVAACRPHARDGRFITAMAAVA
                     GSVAEELIGAFDEAGIARASINNGGDIALHLGPSASFDVGVVADPAIGRAAVAVDGHF
                     RIDAASPVRGIATSGWRGRSFSLGIADSVTVLAASASVADAAATVIANAVDIADARIV
                     RRPANEFRDDSDLGGRLVVRGVPALAPEAIGRALTAGAAVARGERTGGRIIAAVLSLQ
                     GRFLVVDADGPRAVAAAASRAFFPAADPERSFVHADRRHAHAA"
     gene            1108..1713
                     /locus_tag="VFF72_08975"
     CDS             1108..1713
                     /locus_tag="VFF72_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HZV93337.1"
                     /translation="MLIAATPTLPEVDVRKLVVAVEDVWHDNGPRLAQPLRRGSVAAV
                     VRNPYAGRYVEDIQPMMEALKPLGREMAARLVEALGGASRIEGYGKGALIGLDGELEH
                     GALWHVPGGYGMRDLLEGSHAIVPSTTKIGPAGASIDIPIHHRVAAYVRSHFDSMEVR
                     IADAPRPGEMLLVIGMTTGPRPHARVGGLRADEIAKFDGQR"
     gene            1814..>2624
                     /locus_tag="VFF72_08980"
     CDS             1814..>2624
                     /locus_tag="VFF72_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93338.1"
                     /translation="MFKSLAFALTLAAGVAAPFAHADGTIKVGEMNSYKSQPAFTDGY
                     KKGWELAVEQVNDAGGVLGKKLEVISRDDNANPGDAVRVAEELVTREHVSLICGTFLS
                     NVGLAVTDFAKQKKVFFLASEPLTDKITWQDGNRYTFRLRPNTYMQVAMLVPEAVKLH
                     LKRWALVYPNYEYGQSAAATFKAMMKKAQPDVEFVAEQATPLGRIDAGPVTQALADAR
                     PDAIFNVLFGADLIKFVREGTSRDLFKGRRVVSLLTGEPEYLEPLKNEAPVG"
BASE COUNT          368 a          951 c          914 g          391 t
ORIGIN      
        1 gaattcacga tgcggctgga cgactatcgc gcgctcggtg gccatgtcgg tcgcgtccgg
       61 cgcctcgagg acgtcctcgc gacgacagcg catcgcgtcg tcgcgcaggt ggacggcaac
      121 ccgtggccgc tgtcggcgga tctggcgccg gccagcggcg cgatcgcggg gcgcccgtcg
      181 tgagcactgc gacgcgtcgc ccgctcggcg acggccgctg gctcttccag cacgggccga
      241 tcgactgcgt gatccttgcc gaaggccggc ccgaggtggt ggcggcgtgc cgcgagcgcg
      301 cctggcagcg ctttcgcggc gtgctcgacg agctcgtcgg cgagctgccg ctgctgcgca
      361 ccgatctcgg ctcggccgcg ggcgcgtgtg tcgcgccggc gggatcgatc gcgcggcgca
      421 tggtcgcggc ctgccggccg cacgcccgcg acggccgctt catcacggcc atggccgcgg
      481 tggccggcag cgtcgccgag gagctgatcg gcgctttcga cgaggcaggc atcgcccgcg
      541 cctcgatcaa caacggcggc gacatcgcgc tgcatctcgg gccttcggcc tcgttcgatg
      601 tcggcgtggt cgccgatccg gcgatcggcc gggccgcagt cgccgtcgac gggcactttc
      661 gcatcgatgc ggcgtcgccg gtccgcggca tcgccacctc cggctggcgc gggcgcagct
      721 tctcgctcgg catcgccgac tcggtgaccg tgctcgccgc cagcgcctcg gtcgccgatg
      781 cggcggcgac ggtgatcgcc aacgccgtcg acatcgccga tgcgcgcatc gtccgccgcc
      841 cggcgaacga gttccgcgac gacagcgacc tcggtggacg gctcgtcgtt cgcggcgtgc
      901 cggcgctcgc gcccgaagcg atcggccggg cgctgacggc cggcgccgcc gtcgcccgcg
      961 gcgaacgcac gggcgggcgc atcatcgctg ccgtgttgtc gctgcaaggg cgctttctcg
     1021 tcgtcgacgc cgacgggcct cgcgccgtcg cggcagcggc cagccgcgcc ttcttcccgg
     1081 ccgccgaccc cgaacggagc ttcgtccatg ctgatcgccg ccacgcccac gctgcctgag
     1141 gtcgacgttc gcaagctcgt cgttgccgtc gaggacgtct ggcacgacaa cggtccgcgc
     1201 ctggcccagc cgcttcgccg cggcagcgtc gccgcggtcg tgcgcaatcc ctatgctggc
     1261 cgctatgtcg aggacatcca gccgatgatg gaagcgctca agccgctcgg ccgcgagatg
     1321 gcggcgcggc tggtcgaggc gctcggcggc gcgtcgcgga tcgagggcta cggcaagggg
     1381 gcgttgatcg gcctggacgg cgagctcgag cacggcgcgc tctggcacgt gcccggcggc
     1441 tacggcatgc gcgacctgct cgagggatcg cacgcgatcg tcccgtcgac gaccaagatc
     1501 ggcccggccg gcgcgtcgat cgacatcccc atccaccatc gcgtcgccgc ctacgtgcgc
     1561 agccatttcg actcgatgga ggtgcgcatc gccgacgcgc cgcggccggg cgagatgctg
     1621 ctcgtgatcg gcatgacgac cggcccgcgc ccgcacgcgc gcgtcggcgg cctgcgggca
     1681 gacgagattg cgaagttcga cgggcagcgc tagcgtgcga tgcgcggcac gaaggacgcg
     1741 tcgtctgccc gccgacggtc acgacgcctt cattgacgtt cgagcggatt tccatcgcca
     1801 accaggagtc ttcatgttca agagccttgc tttcgccctg accctcgcgg ccggcgtcgc
     1861 cgcgccgttc gcgcacgcgg acggcacgat caaggtcggc gaaatgaaca gctacaagtc
     1921 ccagcctgcc ttcaccgacg gctacaagaa gggctgggag ctcgccgtcg agcaggtgaa
     1981 cgatgcgggc ggcgttctcg gcaagaagct cgaggtcatc tcgcgcgacg acaacgccaa
     2041 tccgggcgac gcggtgcgcg tcgccgagga actggtgacg cgcgagcacg tgtcgctgat
     2101 ctgcggcacg ttcctgtcga acgtcggcct ggccgtcacc gacttcgcca agcagaagaa
     2161 agtcttcttc ctcgcctccg agccgctcac cgacaagatc acctggcagg acggcaatcg
     2221 ctacaccttc cgcctgcgcc cgaacaccta catgcaggtc gcgatgctgg tgcccgaagc
     2281 cgtgaagctg cacctgaagc gctgggcgct cgtctacccc aactacgagt acgggcagtc
     2341 ggcagcggcg accttcaagg cgatgatgaa gaaggcccag cccgacgtcg aattcgtcgc
     2401 cgagcaggcg acgccgctcg gccgcatcga cgccggcccg gtgacgcagg cgctggccga
     2461 cgccaggccc gatgcgatct tcaacgtcct cttcggcgcc gacctcatca agttcgttcg
     2521 cgaaggcact tcgcgcgatc tcttcaaggg ccgccgggtc gtgagcctgc tcaccggcga
     2581 gcccgagtac ctcgagccgc tgaagaacga ggcaccggtc ggct
//
LOCUS       DASRWS010000475         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3928857, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000475 DASRWS010000000
VERSION     DASRWS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928857"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VFF72_08985"
     CDS             complement(<1..1477)
                     /locus_tag="VFF72_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HZV93339.1"
                     /translation="MHRGKGSSVSRCRTMRIRTAVAAASTSAVVALVGCASPGSMTSA
                     NLATAPVTPLAAARPGTSIACADLTARFSFPRTVLTSATPVAAGQLAAAGAPMPAHCR
                     VAGTMNDRTSPVDGKHYAIGFEMRLPQEWNGRFYYQANGGVDGNVVPATGLATAGPAS
                     ASALAKGFAVISSDAGHAAAQNGSFGIDPQARLDYGYQAVAALTPMAKALIAAAYGRP
                     PDRSYIGGCSNGGRHALVAAARMPDAYDGFLAGDPGTVLPRAAIANLLGGKTYAGLAT
                     DPHDIGSGFTAAERRMVSAAVLARCDGLDGAVDGMVQDTRACQAAFDLDRDVPTCTGD
                     RDGSCLSAAQKAGIGKLFAGVTTASGQKVFASFPYDAGLGTNEWAGWKFVSPATRDAG
                     AIADIWRVPPAPPVGFDGRAFMLAANADALLASVQATSATYPESAMSFMLPPHLDDFS
                     AVRRHGGRIVVYHGTSDPIFSSQSSVEVYEGWSRGSGAPAST"
     gene            complement(1567..1986)
                     /locus_tag="VFF72_08990"
     CDS             complement(1567..1986)
                     /locus_tag="VFF72_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZV93340.1"
                     /translation="MQKLLGYSLAQAAIPGLRIFNKHIGEPFQLRRLEFTILMLIASN
                     PHVTQRQMSLALDVSAPRLTIICDKLVDRGLITRTRSEDDRRKQYIALTPKGETLVRK
                     TDRIAETMERELLGHLSELERNLLFQLLDKVAHQRRV"
     gene            complement(2208..>2551)
                     /locus_tag="VFF72_08995"
     CDS             complement(2208..>2551)
                     /locus_tag="VFF72_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HZV93341.1"
                     /translation="STLPANGTVKDFVRLFAVPGMNHCSGGPATDSFPAFTALTNWVE
                     NGQAPDMLVAQSSTTAPATYGWTLPAGATTRVRPLCPYPQYARYIGATGSSAAALAQQ
                     NSPSSYVCTTL"
BASE COUNT          358 a          848 c          939 g          406 t
ORIGIN      
        1 atgtcgaggc cggcgcgccg ctgccgcggc tccagccttc gtagacctcg acgctgctct
       61 ggctcgagaa gatcggatcg ctggtcccgt ggtagacgac gatgcgtccg ccgtggcggc
      121 gcaccgccga gaagtcgtcg agatgcggcg gcagcatgaa ggacattgcc gactccgggt
      181 aggtcgcgct ggtggcctgg accgaggcga gcaaggcgtc ggcattggcc gcgagcatga
      241 aggcccgacc gtcgaatccc acgggcggcg ccggcggaac acgccagatg tcggcgatcg
      301 cgccggcgtc ccgcgtcgcc ggagagacga acttccagcc ggcccactcg ttcgtcccca
      361 ggccggcgtc gtacggaaag gacgcgaaca ccttctgccc ggacgctgtc gtcacgcccg
      421 cgaacagctt gccgatcccc gccttttgcg ccgcactcag gcagctgccg tcccggtcac
      481 ccgtgcaggt cggcacatcg cgatcgaggt cgaatgccgc ctggcaggcc cgggtgtcct
      541 gcaccatgcc gtcgaccgcg ccgtcgaggc cgtcgcaccg ggcgagcacc gccgccgaga
      601 ccatgcgtcg ctcggcggcg gtgaagccgc tgccgatgtc gtgcggatcc gtggccaggc
      661 cggcgtaggt cttgccgccg agcaggttgg cgatcgcggc gcgaggcagc accgtgcccg
      721 gatcgccggc gaggaagccg tcgtaggcgt ccggcatccg ggccgcggcg accagcgcgt
      781 gacggccgcc gttcgagcag ccgccgatgt aggagcgatc gggcggccgg ccgtacgccg
      841 cggcgatcag cgccttggcc atcggcgtca acgcggcgac ggcctggtag ccgtagtcga
      901 ggcgcgcttg cggatcgatg ccgaagctgc cgttttgcgc cgcggcgtgg ccggcgtcgg
      961 agctgatgac cgcgaagccc ttggccagcg cgctggcaga agccggcccc gctgtcgcca
     1021 gtccggtggc cggcacgacg ttgccgtcga cgccgccgtt ggcctggtag tagaagcggc
     1081 cgttccattc ctggggcagg cgcatctcga agccgatcgc gtagtgcttg ccgtcgaccg
     1141 gactggtgcg gtcgttcatc gttccggcga cgcgacagtg cgccggcatc ggcgcgccgg
     1201 cggcggcgag ctggccggcc gcgacgggcg ttgccgaggt gaggacggtg cgcggaaacg
     1261 agaagcgcgc cgtcaggtcg gcgcacgcga tcgaggtgcc gggccgggcc gcggcgagcg
     1321 gcgtcacggg cgcggtcgca aggttcgcgg aggtcatgct gccggggctc gcgcagccga
     1381 ccagcgcgac gacggcgctc gtcgaggcgg ccgcgacggc cgtgcggatg cgcatggtcc
     1441 ggcagcggct gaccgagctt cccttgcccc gatgcatgga agccactttt tcggacgctc
     1501 gattcctgat ttgcatgaca tctccttgcc gatcgaacga ggcaacaacg aatcgccagc
     1561 ctgcttctag acgcgtcgct ggtgcgccac cttgtcgagg agctggaaca ggaggtttcg
     1621 ctcgagttcg ctcaggtggc cgagcaactc gcgctccatc gtttcggcaa tgcggtcggt
     1681 cttgcgcacc agcgtttcgc ccttgggcgt caaggcgatg tattgcttgc ggcgatcgtc
     1741 ttcgctgcgg gtgcgcgtga tcaagccgcg atcgacgagc ttgtcgcaga tgatcgtcag
     1801 gcgcggcgcc gacacgtcga gcgccagcga catctggcgc tgggtgacgt gcggattcga
     1861 cgcgatgagc atcaggatcg tgaattcgag ccgacgaagc tggaacggct cgccgatgtg
     1921 cttgttgaag atgcgcaggc ccgggatggc ggcctgcgcg aggctgtagc cgagcagctt
     1981 ctgcatcctg ccgtgatcca gacggccggg caaccccgac tctcggggtt tcgtcgatga
     2041 cgctccgtca tccgctgcgt gggttgcgcc gcgcggcgtc gcggtggttc gtgggcgcga
     2101 attcgaggct gccgacgcgg tcatcgacag cgagtgtagg ggccagccgg gtcgccaagc
     2161 gagcgggccg cggctcttcg aagccgcggc ccgcccgggt tcatggccta cagcgtcgtg
     2221 cagacgtagc tgcttgggct gttctgctgc gccagtgccg ccgcgctgct tcccgtcgca
     2281 ccgatgtagc gggcgtattg gggatacgga cagagcgggc gcacccgggt cgtcgctccg
     2341 gccggcagcg tccagccata cgtcgccggt gcggtggtgc tcgactgggc gaccagcatg
     2401 tcgggcgcct ggccgttctc gacccagttc gtcagcgccg tgaacgcggg gaagctgtcg
     2461 gtcgccgggc cgccgctgca gtggttcatc ccggggaccg caaagagacg cacgaagtcc
     2521 ttcaccgtgc cattggccgg cagcgtgctc a
//
LOCUS       DASRWS010000476         6143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4215416, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000476 DASRWS010000000
VERSION     DASRWS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4215416"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFF72_09000"
     CDS             complement(<1..537)
                     /locus_tag="VFF72_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZV93342.1"
                     /translation="MSASSPGLVRRFLRGTWRVVDVSRRVVLNLLFLLIVIVILVGLA
                     RSGPAPLADKTTLVLNLDGSITEQRAGNLRATAIDRLRGEVPRKIQLREVLSALDTAA
                     HDDHITNVVLLLDDMDATGLATLREIGAAVDRFRATGKKAIAWGSSYDQRQYLIAAHA
                     DQVYLHPLGSVDITGFGGV"
     gene            717..3383
                     /gene="mdeB"
                     /locus_tag="VFF72_09005"
     CDS             717..3383
                     /gene="mdeB"
                     /locus_tag="VFF72_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HZV93343.1"
                     /translation="MTRPSLNRTPGALRADEAADPDPGETAEWRDALDALSSAQGAGR
                     ARFILDALLAHARRSGVNWLPSMVTPYVNSIALEAQPPFPGDLAMEQRLAALMRWNAL
                     AMVMRANRAETGGSAELGGHIASYASAADLFEVGFNHFFRARNDARGQLGDLVFFQPH
                     SAPGVYARAFLEGRLGADDLAHFRRELVAPAHGARGLTSYPHPQLMPDFWQFPTGSMG
                     IGPINAIYQARFMRYLADRGLLAAEARPEQQRRVWGFFGDGEMDEPESIAALSLAARE
                     QLDNCTFVINCNLQRLDGPVRGNGRIVDELESLFAGAGWHVVKCLWSSDWDVLFARDH
                     GHALMRAFAHTVDGQFQTLSAKDGAFNREVFFGQNAALKALVADLRDEDIDRLRRGGH
                     DAVKIHAAFAAALGHAGQPTVVLAKTMKGYAMGGAAQGRMTTHSAKKLDGADLLAFRD
                     RFALPMSDADTQSLAFYKPADDSAELKYLHSRRRALGGFLPARRSDCPPLAVPPLEST
                     AGFALNTDGKEMSTTMAFVRLLGNLMKDPVLGPRVVPIVADEARTFGMASLFRQVGIY
                     APFGQLYQPEDSGSILTYREERNGQILEEGISEAGALSSWTAAATSYSVHGLPMLPFY
                     IYYSMFGFQRVGDLIWAAADQRARGFLIGATAGRTTLGGEGLQHQDGSSHLVASTVPN
                     CRAWDPATAAELAVIVDHGMREMLVEQKDVFYYVTAMNENIAQPSLPADAHEALLRGL
                     YRFRASSLDSPRRVQLLASGAMLGEAIKAAAMLESGHRVAADLWSVTSFSELARAGVA
                     AEAAWRHGATDVVHSHVGEQLAPTRGPIVIATDYVRAVPEQIRAFLPPGRRCVTLGTD
                     GFGRSDTRAALRAHFEVDAAAIAGAALRALGL"
     gene            complement(3424..4251)
                     /locus_tag="VFF72_09010"
     CDS             complement(3424..4251)
                     /locus_tag="VFF72_09010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZV93344.1"
                     /translation="MASVDPRLISLLTSTARLCAWLLLLSAIFLPLERLFALHSQKFF
                     RKGLAQDLGYFFINGLLPSLLLALPLSLVAVAARAAVPSQMQAEVAAWPLWQRILIGL
                     VVGEIGFYWGHRWAHEIPFLWRFHSLHHSAENIYFLISSRAHPLDNVFIRLCGLIPAV
                     LLGVASPLAPTGSLVPIVIVLVATTWGFFIHSNVRWRLGPLEWLVATPAFHHWHHTLG
                     PQRDCNFASMLPWVDRLFGTFYLPRGRWPARYGIDEKLPASLGAQLVHPFRRSPAPE"
     gene            complement(4334..5155)
                     /locus_tag="VFF72_09015"
     CDS             complement(4334..5155)
                     /locus_tag="VFF72_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZV93345.1"
                     /translation="MRTRLLKATVAGALCVVAYSAQADFLPNVNNLNFLDYAGNAPKG
                     YFTDVNPTGWVGGGGLIFVDAPGTADDGSYLSVYGPFPTGAPTGGNFVEADGNPDYEG
                     SFSQMITGLTAGTTYTLSFYQAGGQQAGFGNGLSTTERWIVSLGDAGLSLCHNCGPID
                     PVYGQTSTYFNTDSNASIAASQLMTTPSGGVTPWQYVSVDLTAHSSSELLSFLAWGDN
                     GSTINLPPIVFLSGVNSVNVLPAVPEPSTWALLGIGALALVAMRRKQRKGFDALA"
BASE COUNT         1030 a         2096 c         2118 g          899 t
ORIGIN      
        1 cacgccgccg aagccggtga tgtcgaccga gccgagcgga tgcaggtaga cctggtcggc
       61 atgcgcggcg atcaggtact ggcgctggtc gtagctcgag ccccaggcga tcgccttctt
      121 gccggtggcg cgaaagcggt cgaccgcggc gccgatttcg cgcagggtgg ccaggccggt
      181 cgcgtccatg tcgtcgagga ggaggacgac gttggtgatg tggtcgtcgt gcgccgccgt
      241 atcgagcgcc gagaggacct cgcgtagctg gatcttgcgc ggcacctcgc cgcgcagccg
      301 gtcgatcgcg gtcgcgcgca ggtttccggc gcgctgctcg gtgatcgagc cgtcgaggtt
      361 gagaacgagg gtcgtcttgt cggcgagcgg cgccggcccg ctgcgggcca gtccgaccag
      421 gatgacgatg acgatgagca ggaagagcag gttgaggacg acgcgccggc tgacgtcgac
      481 gacccgccag gtgccacgaa ggaaccggcg caccaggccg ggtgaagacg cagacatcga
      541 agctccagga cgcgacaaag ctgcatagct taagcgacgg cgtgcctgtc attggaggca
      601 gagcgtggcg cggcgctcgc tttctcgaca cgcccggcgg cggccggcgc ggcgccccgg
      661 ccgaccgggc ctccggcacc ggcgacagcg tccggccacg tggctacgat gccgccatga
      721 cccgcccttc cctgaatcgc acgcccggcg cattgcgggc ggacgaagcc gccgatcccg
      781 accccggcga gaccgccgaa tggcgcgacg ccctcgacgc gttgagctcg gcgcagggcg
      841 cgggacgcgc gcgattcatc ctcgatgcgc tgctcgccca cgctcgtcgc agcggcgtga
      901 actggctgcc gtcgatggtg acgccctacg tcaacagcat tgcgctcgag gcgcagccgc
      961 cctttccggg cgacctcgcg atggagcagc ggcttgccgc gctgatgcgc tggaatgcgc
     1021 tggcgatggt gatgcgcgcc aaccgcgccg agaccggcgg ctcggccgag ctcggtggcc
     1081 acatcgccag ctacgcgtcg gccgcggatc ttttcgaggt cgggttcaac catttcttcc
     1141 gggcccgcaa cgacgcccgc ggccagctcg gcgacctcgt cttcttccag ccgcattcgg
     1201 ctccgggcgt ctatgcccgt gcctttctcg aagggcggct gggcgccgac gacctcgccc
     1261 acttccggcg cgagctcgtc gcgccggcgc acggcgcgcg cggcctcacg tcgtatccgc
     1321 acccgcagct gatgccggac ttctggcagt tcccgaccgg ctcgatgggc atcggcccga
     1381 tcaacgcgat ctaccaggcg cgcttcatgc gctacctcgc cgatcgcggc ctgctcgcgg
     1441 ccgaggcgcg tcctgaacag cagcggcgcg tctggggctt cttcggcgac ggcgagatgg
     1501 acgagcccga gtcgatcgcc gcgctctcgc tcgcggcgcg cgagcagctc gacaactgca
     1561 ccttcgtcat caactgcaac ctgcagcgcc tcgacggccc ggtgcgcggc aacggccgca
     1621 tcgtcgacga gctcgaatcg ctgttcgccg gtgccggctg gcacgtcgtc aagtgcctgt
     1681 ggtcgagcga ctgggacgtg ctcttcgcgc gggaccacgg ccacgccctg atgcgcgcct
     1741 tcgcgcacac cgtcgacggc cagttccaga cgctctcggc caaggacggg gccttcaacc
     1801 gcgaggtctt cttcggccag aacgccgcgc tgaaggcgct cgtcgccgac ctgcgcgacg
     1861 aagacatcga ccgactgcgc cgcggcgggc acgacgcggt gaagatccat gccgccttcg
     1921 ccgccgcgtt gggccatgcc ggccagccga ccgtcgtgct ggccaagacg atgaagggct
     1981 acgcgatggg cggcgccgcg cagggccgca tgacgacgca ctcggcgaag aagctcgacg
     2041 gtgccgacct gctcgcgttt cgcgaccgct tcgcgctgcc gatgagcgac gccgacacgc
     2101 agtcgctcgc cttctacaag ccggccgacg acagcgccga gctgaagtac ctgcacagcc
     2161 gacgccgtgc gctcggcggc ttcctgccgg cacgccgcag cgactgcccg ccgctggcgg
     2221 tgccgccgct cgaatccacc gcaggattcg ccctgaatac cgacggcaag gagatgagca
     2281 cgacgatggc gttcgttcgc ctcctgggca atctcatgaa ggacccggtg ctcgggccgc
     2341 gcgtggtgcc gatcgtcgcc gacgaggcgc gcaccttcgg catggcgagc ctgtttcgcc
     2401 aggtcggcat ctacgcgccc ttcggccagc tctaccagcc cgaggactcg ggctcgatcc
     2461 tcacctaccg cgaggagcgc aacggccaga tcctcgagga aggcatctcc gaagccggag
     2521 cgctgtcgtc gtggacggcg gcggcgacgt cgtactcggt gcacggactg ccgatgctgc
     2581 ccttctacat ctactactcg atgttcggct tccagcgcgt cggcgatctc atctgggccg
     2641 ctgccgacca gcgcgcccgc ggctttctca tcggagcgac ggccggccgc acgacgctcg
     2701 gcggcgaggg cctgcagcac caggacggca gcagccatct ggtcgcctcg accgtgccga
     2761 actgcagggc atgggacccg gcgacggcgg ccgagctcgc cgtcatcgtc gaccacggca
     2821 tgcgcgagat gctggtcgag cagaaggacg tcttctacta cgtgacggcg atgaacgaga
     2881 acatcgcgca gccgtccctg ccggccgatg cgcacgaggc gctgctgcga ggcctgtacc
     2941 gctttcgcgc ctcgtcgctc gattcgccgc gccgcgtcca gctgctggct tcgggggcga
     3001 tgctgggcga ggcgatcaag gccgcggcga tgctcgagtc cgggcatcgc gtcgccgccg
     3061 atctctggag cgtcaccagc ttctcggagc tggcccgcgc cggcgtcgcc gccgaggccg
     3121 cgtggcgaca cggcgcgacc gatgtcgtgc acagccacgt cggcgaacag ctcgcgccga
     3181 cgcgcggccc gatcgtcatc gccaccgact acgtgcgcgc cgtgccggag cagatccgcg
     3241 ccttcctgcc gcctggccga cgctgcgtca cgctcggcac cgacggcttc ggccgcagcg
     3301 acacgcgggc ggcgctgcgc gcgcatttcg aggtcgacgc ggcggcgatc gccggcgccg
     3361 ccttgcgggc actgggcctc tgagcgacgt gcgctcagcg cacgccggag ttgccgaaga
     3421 ccttcactcc ggtgccggcg agcggcgaaa cggatgcacg agctgggcgc ccagcgaagc
     3481 cggcagcttc tcgtcgatgc catagcgcgc gggccaccgg ccacgcggca ggtagaaggt
     3541 gccaaagagc cggtcgaccc acggcagcat cgacgcgaaa ttgcaatcgc gctgtggccc
     3601 cagcgtatgg tgccagtgat gaaacgccgg cgtggcgacc agccactcga gcggacccag
     3661 tcgccagcgc acattggaat ggatgaagaa gccccaggtc gtcgcgacca gcacgatcac
     3721 gatcggcacg aggcttccag tcggcgcgag cgggctcgcg acgccgagca ggacggcggg
     3781 tatcaggccg cacaagcgga tgaagacgtt gtcaagcgga tgggcacgcg aactgatgag
     3841 gaaatagatg ttctcagcgc tgtgatgcaa agagtggaag cgccagagaa agggaatctc
     3901 gtgcgcccag cggtgacccc agtagaagcc gatctcgccg acgaccaagc cgatgaggat
     3961 gcgttgccac agcggccacg cggcgacctc cgcctgcatc tgcgaaggca ccgccgcgcg
     4021 cgccgccacg gcaacgagcg acagcggcaa tgccagcagc aagctcggca ggagaccgtt
     4081 gatgaagaaa taacccagat cctgggccag gcccttgcgg aagaatttct gggagtgcag
     4141 cgcgaagagc cgctcgagcg gaagaaagat cgccgagaga aggagcagcc aggcgcaaag
     4201 cctggccgtg ctggtcagca gcgaaatcag ccggggatcg acggacgcca agaagaattc
     4261 gtccagtgaa gcttcttgcc gcgccgccgc tcggcgacgt ggcgaagcta ttttgccgcc
     4321 gcgcggctat cgatcaggca agcgcgtcga agccctttcg ctgcttcctc cgcatcgcga
     4381 ccagcgccag cgcgccgatg cccagcaacg cccaggtcga cggctcagga acagccggca
     4441 ggacattcac cgaattcacg ccggacagga acacgatcgg tggcaggttg atcgtgctgc
     4501 cgttgtcgcc ccaggcgaga aaactcagca attcgctcga cgaatgcgcg gtgaggtcga
     4561 cggagacgta ttgccagggt gtcacgccac cggatggcgt cgtcatcagt tgcgacgccg
     4621 cgatgctcgc attcgaatcg gtgttgaagt aggtactcgt ctgtccatag acggggtcga
     4681 tggggccaca gttgtggcac aggctcagcc cggcgtcgcc aagcgaaacg atccaacgct
     4741 ccgtggtgga aaggccgttg ccgaatcccg cctgctggcc gcccgcctgg tagaaactta
     4801 aggtgtacgt cgtcccggcg gtcagccccg tgatcatctg gctgaacgag ccttcgtagt
     4861 cgggattgcc gtcggcctcg acgaagttgc cgccggtggg cgcgccggtc gggaacgggc
     4921 cgtagacgct cagatagctg ccgtcgtctg cagtgccggg ggcgtcgacg aagatcaggc
     4981 cgccgccgcc gacccagcca gttggattca catcggtgaa atagcccttc ggcgcgttgc
     5041 cggcgtaatc gaggaaattg aggttgttga cgttgggaag aaagtcggcc tgggccgagt
     5101 aggcgacgac gcagagtgcg ccagcgaccg ttgccttcag tagtctggta cgcattgaat
     5161 cctccaaaga gttcggccga cgaacgcggt cgttgcgaca tgggtgtcga agcgtggcga
     5221 acgatcgtag gcgtctgggg ccgacgcaaa accccgcgaa tgaggggttt accggatccc
     5281 agcgacagcg aattcgcaag cggctcgacg acgactcgcg cgtgctggcc ggccgcgcga
     5341 ttgcggccgc ggcggacgcc ccgcgcctgc cgggcgtggc tcagggatcg tcggatatcc
     5401 ggccctgtct cctcgtggcg atttccgatc ggctcggccg ccgcttcgac gacgcgcgcg
     5461 ggccctgcag cgcgcgaccg cgctggccga gaggctgacg caaagcggat acccgggcag
     5521 cgagttcggg ggccgacgcc aggtgagcaa gcgcagctcg agctggccat aggcctgacc
     5581 ccgcgggcgc gcagtgcggc gtgtggagag gcgactgagc tacggaccgt gatgagcgca
     5641 cgcgctccgg caacgcagtt cacgatgcgg ctcgccgcgt tcggcgatcg tccgccatga
     5701 cgcgaagagt cgcctcgcac cacgcgcgca cgcgtcaccg cgtcgaacgt ccaccaccac
     5761 ggcgatggtt tccgccaggc ggcagtcgga ccggaaacgg cagccgctca gcgaagcggc
     5821 gtcgctgcag cgcgaatgcc agcatccggt gacctcgtgc acgactgcag gcaacgagca
     5881 tcgacttgcc gggtcggcga cgtcgaccgc tgagcgggag gcgccaatcg ataccgtcgt
     5941 caaggcaatc cgcttcgtca cgatcctgcg tgcagacgcg gcacgtgagc atcgcgatcc
     6001 agctgactga aggcaacgcg gcgttcgtgc gtcacagctg gaacgccagc cagagcagca
     6061 agccttccga gagatcgcgc agcagccccg gctccttcgg ctcgaagcga gccgagcgca
     6121 actgctcgtt ctcgatccac tcg
//
LOCUS       DASRWS010000477         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_783227, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000477 DASRWS010000000
VERSION     DASRWS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783227"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFF72_09020"
     CDS             complement(<1..1056)
                     /locus_tag="VFF72_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV93346.1"
                     /translation="MLITHEHEEIRRTLKRFIDDEINPHVDEWEAAEAFPAHEVFKGL
                     GKLGLLGLTKPEKYGGMALDYSYSVVMAEALGHAACGAVPMAIGVQTDMCTPALARFG
                     SEALCREFLVPAITGDAVGCIGVSEPGAGSDVAAIRTTARKDGGDYVINGAKMWITNS
                     LQADWMCLLANTGEGPAHKNKSLIMVPMNTPGVTRAKKIRKIGMMASDTGLIHFDDVR
                     VPQSHRIGEEGLGFTYQMMQFQEERLWAAANAIESLSGCIRQTIDYARERQIFGQSVL
                     DHQVVHYKLAELKTEVEALRGLVYMATEKYIAGSDVTEWASMAKLKACRLGRLVPDAC
                     MQYWGGMGYTWDNPVSRL"
     gene            complement(1081..2694)
                     /locus_tag="VFF72_09025"
     CDS             complement(1081..2694)
                     /locus_tag="VFF72_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZV93347.1"
                     /translation="MVASTPASIDADDARRARRSHTLALIERWRGLEARTREASARAA
                     PLFNRRGQILPRERVARLLDPGVPFLELSTMAGWLLDTDDAGRSVPGGGVIAGIGVVS
                     GVRVMLVADDAGIDAGALQPMGLEKLQRAQAIALENKLPFVHLVESAGANLLAYRVEQ
                     FIHGGSAFYNLARLSAAGIPVIAVVHGSSTAGGAYMTGLSDHVIMVRGRGRAFLAGPP
                     LLKAATGEVAGDEALGGADMHASISGLVEHVADDDASALATARSLITSLGWQPPASWP
                     DGPGPIGSADDLLDLFGADMKRPVEMRRVVERIVDASEFLEFKAGYGAATVCGYAAIA
                     GYRVGLVTNNGPIDPAGSTKTTHFIQTCEQAGLPIVWLQNTTGFIVGTEAERAGMIKH
                     GSKQIQALSNATVPQITVQCGASFGAGNYGMCGRGFHPRFVFSWPTARTAVMGAEQAA
                     GTMEIVMRDGAARKGEPVDEAKLATLKARITATFERQSDAFYTSGRLLDDGLIDPRDT
                     RRVVALCLAVCDEATRRTLHPMQFGVARP"
     gene            complement(2694..>3239)
                     /locus_tag="VFF72_09030"
     CDS             complement(2694..>3239)
                     /locus_tag="VFF72_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93348.1"
                     /translation="GWDAVVRNNLTGGFLMARECITQWMLDDDGSGAARGGAIVNIIA
                     DMWNGMPGMGHSGAARAGMLNLPETAALEWAPVRVNAVAPGWIASSGMDRYPPEMRDT
                     IRGMKALVPLRHLGTESEVAATIVFLLSPAAAFVSGACLRVDGAAPNAKRIWPDVGTG
                     GTTAAFDGFHLAATPAVLKDG"
BASE COUNT          454 a         1123 c         1153 g          509 t
ORIGIN      
        1 caggcgcgac accgggttgt cccaggtgta gcccatgccg ccccagtact gcatgcaggc
       61 gtcggggacg aggcggccga gccggcaggc cttcagcttg gccatcgacg cccattcggt
      121 gacgtcgctg ccggcgatgt atttctcggt ggccatgtag acgaggccgc gcagggcctc
      181 gacctcggtc ttcagttcgg cgagcttgta gtggacgacc tggtgatcga ggacgctctg
      241 gccgaagatc tgccgctcgc gggcgtagtc gatcgtctgc cggatgcagc ccgacagcga
      301 ctcgatcgcg ttggcggccg cccagaggcg ctcctcctgg aactgcatca tctggtaggt
      361 gaagccgagc ccttcctcgc cgatgcgatg gctctgcggc acgcgaacgt cgtcgaagtg
      421 gatgaggccg gtgtcgctgg ccatcatgcc gatcttccgg atcttcttcg cccgcgtcac
      481 gccgggcgtg ttcatcggca ccatgatcag gctcttgttc ttgtgcgccg gcccctcgcc
      541 ggtgttggcg agcaggcaca tccagtcggc ctgcaggctg ttcgtgatcc acatcttggc
      601 gccgttgatg acgtagtcgc cgccgtcctt cctggccgtc gtcctgatcg ccgcgacgtc
      661 ggagccggcg cccggctcgg agacgccgat gcagccgacc gcatcgcccg tgatcgccgg
      721 gacgaggaat tcgcggcaca gggcctcgct gccgaatcgc gccagcgcgg gcgtgcacat
      781 gtcggtctgt acgccgatcg ccatcggcac cgcaccgcag gcggcatggc ccagggcttc
      841 ggccatgacc accgagtacg aatagtcgag cgccattccg ccgtacttct cgggcttggt
      901 gaggccgagc aggccgagtt tgcccaggcc cttgaagacc tcgtgcgcgg ggaatgcctc
      961 ggcggcttcc cattcatcga cgtgcggatt gatctcgtcg tcgatgaagc gcttcagggt
     1021 gcggcggatc tcttcgtgct cgtgggtgat cagcatcggc tctccttctc gcggcgcgcc
     1081 tcagggacgg gcgacgccga actgcatcgg atgaagtgtg cgccgagtcg cctcgtcgca
     1141 gaccgccagg cagagcgcga cgacgcggcg cgtgtcgcgc gggtcgatca ggccgtcgtc
     1201 gaggaggcgg cccgaggtgt agaacgcgtc gctctgccgc tcgaaggtgg ccgtgatcct
     1261 cgccttcagc gtcgcgagct tcgcctcgtc gaccggctcg cccttgcgcg cggcgccgtc
     1321 gcgcataacg atctccatcg tccccgcggc ctgctcggcg cccatgacgg ccgtccttgc
     1381 ggtcggccac gagaagacga agcgcggatg gaagccgcgc ccgcacatgc cgtagttgcc
     1441 ggcgccgaac gacgcgccgc attgcacggt gatctgcggc accgtcgcgt tcgagagcgc
     1501 ctggatctgc ttcgagccgt gcttgatcat gccggcgcgc tcggcctcgg tgccgacgat
     1561 gaagccggtc gtgttctgca accagacgat cggcaggccc gcctgctcgc aggtctggat
     1621 gaagtgggtc gtcttggtgc tgccggccgg gtcgatcggg ccgttgttgg tgacgaggcc
     1681 gacgcgatag ccggcgatcg ccgcgtagcc gcagaccgtc gccgcgccgt agccggcctt
     1741 gaattcgagg aactccgacg catcgacgat gcgttcgacg acgcggcgca tctcgaccgg
     1801 gcgtttcatg tcggcaccga agaggtcgag caggtcgtcg gccgacccga tcgggcccgg
     1861 gccgtcgggc caggatgccg gcggttgcca gccgagcgag gtgatcagcg agcgcgcggt
     1921 cgccagggcg ctcgcgtcgt cgtcggcaac gtgctcgacc aggccggaaa tgctcgcgtg
     1981 catgtcggcg ccgccgagcg cctcgtcgcc ggcgacttcg ccggtcgccg ccttgagcag
     2041 cggcggcccg gcaaggaagg cgcggccacg gccgcggacc atgatcacgt ggtccgacag
     2101 gcccgtcatg taggcgccac cggccgtcga cgacccgtgg acgacggcga tcaccgggat
     2161 ccccgccgcg gacagccgcg ccaggttgta gaacgcgctg ccgccatgga tgaactgctc
     2221 gacgcgatag gcgagcaggt tggcgccggc cgactccacg agatgcacga acggcagctt
     2281 gttctcgagg gcgatcgcct gcgcgcgctg cagcttttcg aggcccatcg gctgcagcgc
     2341 gccggcgtcg atgccggcgt cgtcggcgac cagcatgacg cgaacgcccg agacgacgcc
     2401 gatgccggcg atgacgcccc cgcccggcac cgagcggccg gcatcgtcgg tgtcgagcag
     2461 ccagccggcc atcgtcgaca gctcgagaaa cggcacgccc ggatcgagca gccgggcgac
     2521 gcgctcgcgc ggcaggatct ggccgcggcg gttgaagagg ggcgccgccc gggcgctcgc
     2581 ttcgcgagta cgcgcttcga ggccgcgcca gcgctcgatc agcgccagcg tgtgcgagcg
     2641 acgcgcgcgg cgggcgtcgt cggcgtcgat cgatgccggc gtcgaggcga ccatcagccg
     2701 tccttcagca cggccggggt cgccgcgaga tggaagccgt cgaacgccgc ggtcgtgccc
     2761 ccggtgccca cgtcgggcca gatgcgcttg gcgttcggcg ccgccccgtc gacacgcagg
     2821 caggcgcccg agacgaacgc ggccgcgggt gaaagcagga agacgatcgt cgcggcgact
     2881 tcgctctcgg tgccgaggtg gcgcagcggc accagcgcct tcatgccgcg gatggtgtcg
     2941 cgcatctcgg gcgggtagcg gtccatgccg ctcgaggcga tccagcccgg ggcgacggcg
     3001 ttgacgcgca ccggcgccca ttcgagcgcc gccgtctcgg gcaggttgag catgcccgcg
     3061 cgcgcggcac ccgagtggcc catgccgggc atgccgttcc acatgtcggc gatgatgttg
     3121 acgatcgcgc cgccgcgcgc cgcgccgctg ccgtcgtcgt cgagcatcca ctgcgtgatg
     3181 cactcgcggg ccatcaggaa cccgccggtc aggttgttgc gcaccaccgc gtcccagcc
//
LOCUS       DASRWS010000478         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_486826, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000478 DASRWS010000000
VERSION     DASRWS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486826"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFF72_09035"
     CDS             <1..220
                     /locus_tag="VFF72_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93349.1"
                     /translation="RRTRQPTTTVTAKIFSNTGRSGTAKALAPADGSARRGGLAGLHV
                     SSSNRVVRIDAGRTRRSDSIGDAAEWVA"
     gene            208..1464
                     /locus_tag="VFF72_09040"
     CDS             208..1464
                     /locus_tag="VFF72_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV93350.1"
                     /translation="MGRVSSPVATSGVDSPAAWRRLVVALTLMTIGSSAMYVVSVALP
                     SVQKEFGVARADASLPYTLLMVGFGIGGIFMGRIADRRGIVLPSLIGAVGLAAGFILA
                     GLSTNLTGFALAHGVLLGLVGSSFTFAPLVADTSLWFAKRRGIAVAICASGIYVAGAI
                     WPPIVQHFVETVGWRQTYIGLGIFCAVALPPLAMLMRTPPPVPSRGTGTERAGPTPST
                     RPFGLSMNAAQALLCIAGVSCCVAMAMPQVHIVAYCGDLGFGAARGAQMLSLMLGCGI
                     VSRLVSGAICDRIGGLRTLLLGSVLQGTALLLFLPFDSLASLYVISALFGLFQGGIVP
                     SYAIIVREHFPAAEAGARVGTVLMCTLLGMALGGWMSGKVFDLTGSYHAAFLNGLAWN
                     ALNVSIALFLLGRLHVTTARRRAIAA"
     gene            1492..2412
                     /locus_tag="VFF72_09045"
     CDS             1492..2412
                     /locus_tag="VFF72_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZV93351.1"
                     /translation="MTILVGTASWTDKTLIACGRFYPKDATSAEARLRFYASRFPIVE
                     VDSSYYAMPAPATAQLWAERTPEGFVMNVKAFRLFTGHPTTAVVMHRDLQPAFAGLGA
                     RFFYRDLGSALVDELWRRFREALLPLRAAGRLGLVHFQFPPWLRCEAPSRAHVEMCVE
                     HMAGFDLAIEFRHESWFAGASRQRETLDFLRELGVAHTVVDGPQGFYNSVPALWEATH
                     PRFALLRLHGRNTATWNVKGQTSASDRFNYDYPDDELAGLVPRLEQLALTAMQTHVIF
                     NNNMEDQGQRNARSLIDLLAAAGVGGGPKA"
     gene            2673..3005
                     /locus_tag="VFF72_09050"
     CDS             2673..3005
                     /locus_tag="VFF72_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93352.1"
                     /translation="MSTARSAALRETSAWRVALFAAGLTILLWLVLWGASLLSDNGRI
                     PSELAAAAQQPGDMVCADGAIVRGDGSFVDSLFSTGGRFRCTDWRMRHPRVDPSTGKT
                     DWPSSPLR"
     gene            complement(3039..>3533)
                     /locus_tag="VFF72_09055"
     CDS             complement(3039..>3533)
                     /locus_tag="VFF72_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HZV93353.1"
                     /translation="SYDYIAPQTFRVSSTAVSLGSTAQWQLTDALSLQGTAMGGVGYA
                     AVGTTRGAGGDRDYNYGLAPQALVALRLTDSDKASVDLTGREYFVSSVASGTSGGHDN
                     ILRGDAAFTYRIARQQAITLKVTASRRDARFVDPAAQRQTQVTVGLFYTLLGQDRFGT
                     VDWR"
BASE COUNT          474 a         1281 c         1183 g          595 t
ORIGIN      
        1 caggaggacc agacaaccga cgacgacggt gacggcgaag attttttcga acacggggcg
       61 gagcggcacc gcgaaggcgc tcgctccggc agatggtagc gcgcgccggg gcggcctggc
      121 tggccttcac gtgtcttcgt cgaaccgggt ggtccgcatc gacgcgggcc gcacgcgacg
      181 gtcggacagc atcggcgatg ccgcagaatg ggtcgcgtga gctcgcccgt cgcaacgtcc
      241 ggagtcgatt cgcccgcggc gtggcgacgg ctggtggtcg cgctgacgct gatgacgatc
      301 ggcagcagcg ccatgtacgt cgtctcggtc gcgctgcctt cggtgcagaa agagttcggc
      361 gttgcgcgcg ccgatgcctc gctgccctac acgctgctga tggtcggatt cggcatcggc
      421 ggcatcttca tgggtcgcat cgccgatcgg cgcggcatcg tcctgccgag cctgatcggc
      481 gcggtcgggc tcgccgccgg cttcatcctc gccggcctga gcaccaacct gaccggtttc
      541 gcgctggctc acggcgtgct gctcggcctc gtcggcagct ccttcacctt cgcgcccctc
      601 gtcgccgaca catcgctctg gttcgcgaag cgccgcggca tcgccgtcgc gatctgcgcc
      661 agcggcatct acgtcgccgg cgcgatctgg ccgccgatcg tccagcactt cgtcgagacg
      721 gtcggctggc gccagaccta catcggcctg ggcatcttct gcgccgtggc gctgccgccg
      781 ctggcgatgc tgatgcgaac gccgccaccg gtgccgtcac gcggcaccgg gacggaacga
      841 gccggcccga caccttcgac gcggccgttc gggctgtcga tgaacgcggc gcaggcgctg
      901 ctctgcatcg ccggcgtgtc ctgctgcgtc gcgatggcga tgccgcaggt ccacatcgtc
      961 gcctactgcg gcgatctcgg cttcggcgcc gcgcgcggtg cgcagatgct ctcgctgatg
     1021 ctcggctgcg gcatcgtcag ccggctcgtc tcgggcgcca tctgcgaccg catcggcggc
     1081 ctgcgcacgc tgcttctcgg ctcggtgctg cagggcaccg ccctgcttct cttcctgccc
     1141 ttcgattcgc tggcctcgct ctatgtcatc tcggcgctgt tcggtctctt ccagggcggc
     1201 atcgtccctt cctacgcgat catcgttcgc gagcatttcc ccgccgccga agccggcgcg
     1261 cgcgtcggca ccgtactgat gtgcacgttg ctgggcatgg cgctcggcgg ctggatgtcg
     1321 ggcaaggtct tcgacctgac ggggtcgtac cacgccgcct ttctcaacgg cctggcctgg
     1381 aacgcgctca acgtgtcgat cgccctgttc ctgctcggcc ggcttcacgt cacgaccgcg
     1441 cggcgccgcg cgatcgcggc ctagcgtcgc gccgctgtcg agaaatcccc catgacgatc
     1501 ctcgtcggca ccgcgtcgtg gaccgacaag accctgatcg cctgcggccg cttctatccg
     1561 aaggacgcca cctcggccga ggcccggctg cgcttctacg cgtcgcgttt tccgatcgtc
     1621 gaggtcgatt ccagctacta cgcgatgccc gccccggcga cggcgcaact ttgggccgag
     1681 cgcacgcccg aaggcttcgt catgaacgtg aaggccttcc gcctcttcac cggccacccg
     1741 acgaccgccg tcgtgatgca tcgcgacctg caaccggcgt tcgccggcct cggcgcacgc
     1801 ttcttctatc gcgacctcgg ctccgcactc gtcgacgagc tgtggcgtcg ctttcgcgaa
     1861 gcgctgttgc cgctgcgcgc ggccggacgg ctcggcctgg tccacttcca gttcccgccg
     1921 tggctgcgct gcgaagcgcc gagccgcgcc cacgtcgaga tgtgcgtcga gcacatggct
     1981 ggtttcgacc tggccatcga atttcgacac gagagctggt tcgctggtgc ctcgcggcag
     2041 cgcgagacgc tcgatttcct gcgcgagctg ggcgtcgccc acaccgtggt cgacggaccg
     2101 cagggcttct acaacagcgt gccggcgttg tgggaagcga cgcacccgcg cttcgcgttg
     2161 ctgcgcctgc acgggcgaaa cacggcgacg tggaacgtca agggccagac gtcggcgtcg
     2221 gaccggttca actacgacta tcccgacgac gagctcgccg gcctcgtgcc gcgcctggag
     2281 cagctggcgc tgaccgcgat gcagacgcac gtcatcttca acaacaacat ggaagaccag
     2341 ggccagcgca acgcacgctc gctgatcgac ctgctggcgg cagctggcgt cggcggcggc
     2401 ccgaaagcct gagccgccgc caggctgcgc cgatcgggtc ggtacggcag gacggcagcg
     2461 gaacgccggc cagcttgggc ggctcgacga tgcacgcctt gcccggcgcc aagcgcattg
     2521 gcaggcggct tcgacacgaa ggcgaggttg cgggcggaat ttgcgcattc gctcacgccg
     2581 catccccggc gacttccctg cggtcgcggc gggtcggcgg cggctcctaa gatcgacggc
     2641 ttcgcatcaa gcgcgccacg cacccgagtc gaatgagcac cgcccgatcc gccgctctcc
     2701 gcgagaccag cgcctggcga gtcgccctct tcgcggccgg actgacgatc ctcctgtggc
     2761 tcgtcctctg gggcgcgagt ctcctcagcg acaacggtcg catcccgtcg gagctcgccg
     2821 cggctgccca gcagcccggc gacatggtct gcgccgacgg cgcgatcgtg cgcggcgacg
     2881 gctcgttcgt cgacagcctg ttcagcaccg gcgggcgctt tcgctgcacc gactggcgca
     2941 tgcgccatcc gcgcgtcgac ccgtcgaccg gcaagaccga ctggccgtcc tcgccgctgc
     3001 gctgacgcga gcgcgctcgt cgacgatcgc gggcgccgtc agcgccagtc gacggtgccg
     3061 aagcgatcct gcccgagcag cgtgtagaag aggccgaccg tgacctgcgt ctgccgctgc
     3121 gcagccggat cgacgaagcg ggcgtcgcgg cggctcgccg tgaccttgag cgtgatcgcc
     3181 tgctgacgcg cgatccggta cgtgaacgcc gcatcgccgc gcaggatgtt gtcgtggccg
     3241 ccgctggtgc ccgacgcgac gctgctgacg aaatactccc ggccggtgag gtcgacggag
     3301 gccttgtcgc tgtcggtcag tcgcaacgcc accaaagcct gtggcgccag gccgtagttg
     3361 tagtcgcgat cgccgcccgc tccccgcgtc gtcccgaccg ccgcatagcc gacgccaccc
     3421 atcgctgtcc cctgcagcga cagggcgtcg gtcagctgcc attgcgccgt cgagccgagc
     3481 gatacggccg tgctggagac tcgaaaggtc tgcggcgcga tgtagtcgta gct
//
LOCUS       DASRWS010000479         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1058494, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000479 DASRWS010000000
VERSION     DASRWS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058494"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFF72_09060"
     CDS             <1..936
                     /locus_tag="VFF72_09060"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HZV93354.1"
                     /translation="LRYLDNSQSAGPHSRIVAVAARRAARRGESARVSGLNENLAREV
                     LELHTLGAESARDGIYTQADVTSFAAVLTGWRAPLDSGTGTSVFEAAWHEPGPKTVLG
                     KTYPEGPEALRMVLADLARHPATARFIATKLARHVVADEPPPALVDRLAAVYLRSDGR
                     LSEVYRELIANDLAWKASPVKLKTPEEFVVSTARVLQIGERLFAAADSRPRAGNPAGA
                     AIVGLGQRLQAAPSPAGWSDLADDWLGPDAVWKRIEWATRIADRSGRSIDARRLASQS
                     LGPRLAPATIQQIDRAADGPQALALLLLAPEFQRR"
     gene            958..2178
                     /locus_tag="VFF72_09065"
     CDS             958..2178
                     /locus_tag="VFF72_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZV93355.1"
                     /translation="MHEPKPLATRSARAVDPSRRRVLLAGAATSLLAPWSRLAFAGRA
                     SPTGNRVAFVILRGGMDGLSAVPAVGDPDFASARGPLAEYSTTPLPLDSTLALHPNLV
                     ELHAMFGRGELCVVHAAGLAYRERSHFDAQQVLESGGVRPYELATGWLGRALAASGDK
                     GIALDTAVPLVLRGSPSVDTWAPSALPDPSADLVARVERMYAGDPVLAAALQRASQLH
                     LEPAAMRDDGGTTGPRPGAFVALAERAAGFLAQPDGPQAAVLEITGWDTHANQASPNG
                     PLASGLRQLDAGLAALRNGLLAGDAWHRTAVVVASEFGRTVAINGTLGTDQGTGGVAL
                     VLGGAVRGRVATDWPGLAPGRRFEGRDLRTTTDLRAVLKGVLGDHLKIASQTLDSDVF
                     PGSAKIRPASLLRA"
     gene            complement(2175..>2847)
                     /locus_tag="VFF72_09070"
     CDS             complement(2175..>2847)
                     /locus_tag="VFF72_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HZV93356.1"
                     /translation="KFDLTPSPYLVAEGRAWRSATGDRPVVLAAITRDGEEAMLLAAW
                     RTLAGMRPLLVIVPRHPQRFDEVAALVRAAGFSLSRRSAWPARPDGAAATADVWLGDS
                     MLEMPAYYALADVALLGGSFAPLGGQNLIEAAACGCPVVMGPHTFNFAEAAELAVAAG
                     AAVRVATMDEGVVRAVSLAGSDEQRERSQSGIAFAASHRGAAGRIAVGIEELLASRDR
                     GPGRR"
BASE COUNT          394 a         1096 c          986 g          371 t
ORIGIN      
        1 ctgcgctacc tcgacaattc gcagtcggcg ggcccgcatt cgcggatcgt cgccgtcgcc
       61 gcgcgacgcg ccgcgcgacg aggcgaaagc gcgcgcgtca gcggcctcaa cgagaacctg
      121 gcccgcgagg tgctggagct gcatacgctg ggcgccgaga gcgcccgcga cggcatctac
      181 acgcaagccg atgtgacgtc gttcgccgcc gtcctgaccg gctggcgggc tcccctcgac
      241 agcggcaccg gcaccagtgt cttcgaggcg gcgtggcacg agcccggtcc gaagacggtg
      301 ctcggcaaga cctatcccga agggccggag gcgctgcgca tggtcctcgc cgacctcgcc
      361 aggcacccgg cgacggcgcg cttcatcgcc accaagctcg cgcgccacgt cgtcgccgac
      421 gagccgccgc cggccctcgt cgaccggctc gccgccgtct acctgcgcag cgacggccgg
      481 ctttccgagg tctaccgcga gctcatcgcc aacgacctgg cctggaaggc gtcgcccgtc
      541 aagctgaaga cgcctgagga attcgtcgtc tcgacggcgc gcgtcctgca gatcggcgag
      601 cgactgttcg ctgcagccga cagccggcct cgcgcaggca atcctgccgg cgccgcgatc
      661 gtcggcctcg gccagcgcct gcaggcggcg ccctcgccgg ccggctggag cgatcttgcc
      721 gacgactggc tgggacccga cgcggtgtgg aagcggatcg aatgggcgac ccgcatcgcc
      781 gatcgcagcg gccgatcgat cgatgcgcgc cgactcgcct cgcaaagcct cggcccgcgt
      841 ctggcgccgg caacgataca gcagatcgat cgtgccgccg acggtccgca ggcactcgcg
      901 ctgctgctgc tcgcgccgga attccagcgg cgctgagatc caccagggag actctccatg
      961 cacgaaccca agcccctggc cacgcgcagc gcacgggccg tcgacccgtc gcgccgtcgg
     1021 gtgctgctgg ccggcgcggc cacatcgctg ctggcgccct ggtcccgcct ggcattcgcc
     1081 ggccgcgcca gcccgaccgg caaccgcgtg gccttcgtca tcctgcgcgg cggcatggac
     1141 gggctgagcg ccgtcccggc cgtcggcgac ccggatttcg catcggcgcg aggcccgctc
     1201 gccgagtatt cgacgacgcc gctgccgctc gattcgacgc tggccctgca ccccaacctc
     1261 gtcgagctgc acgccatgtt cgggcgcggc gagctctgcg tcgtccacgc cgccggcctc
     1321 gcctatcgcg aacggtcgca cttcgatgcc cagcaggtgc tcgaaagcgg cggcgtgcgg
     1381 ccttacgagc tggcgacggg ctggctcggc cgcgccctcg ccgccagcgg cgacaagggc
     1441 atcgctctcg acaccgccgt gccgctggtg ttgcgcggct cgccgtcggt cgacacctgg
     1501 gcgccgtcgg cacttcccga tccgtcggcc gacctggtcg cccgggtcga acggatgtac
     1561 gccggcgatc cggtgctggc cgcggcactg cagcgtgcca gccagctgca tctcgagccg
     1621 gcagcgatgc gcgacgacgg cggcacgacc ggtccacgcc cgggtgcgtt cgtcgccctc
     1681 gccgagcgcg ccgccgggtt cctggcccag cccgacggac cgcaggccgc ggtgctcgag
     1741 atcacgggct gggacaccca cgccaaccag gccagcccga acgggccgct cgcctcggga
     1801 ctgcgccagc tcgacgccgg gctggccgcg cttcgcaacg gcctgctcgc cggcgatgcc
     1861 tggcacagga ccgccgtcgt cgtcgccagc gagttcggcc ggacggtggc gatcaacggc
     1921 acgctcggca ccgaccaagg cacgggcggc gtcgccttgg tgctgggcgg cgcggttcgc
     1981 ggccgcgtcg cgaccgactg gccgggcctc gctccggggc gtcgcttcga gggccgcgac
     2041 ctgcgcacca ccactgacct gcgcgccgtg ctgaaaggcg tcttgggcga ccacctgaag
     2101 atcgcttcgc aaacgctcga cagcgacgtg tttcccggca gcgcgaagat ccgacccgcg
     2161 tcgttgctgc gcgcctagcg tcttccaggc cctctgtcgc gggacgccag cagttcctcg
     2221 atcccgacgg cgatgcgccc tgcggcgccg cgatgcgaag cggcgaaggc aatgccggac
     2281 tgcgaccgtt cgcgctgttc gtcgctgccg gccagcgaga cggcgcggac cacgccctcg
     2341 tccatcgtcg cgacacgcac ggcagctccg gctgcgacag ccagctcggc ggcctcggcg
     2401 aaattgaagg tgtgcgggcc catcacgacc ggacagccgc aagcggccgc ctcgatcagg
     2461 ttctgtccgc cgagcggcgc gaagctgccg ccgagcagcg cgacgtcggc cagcgcgtag
     2521 taggccggca tctcgagcat cgaatcgccg agccagacgt cggccgtcgc ggcggcgccg
     2581 tcgggccgcg ccggccacgc actccgcctc gacaacgaaa agcccgccgc cctgacgagt
     2641 gcggcgacct cgtcgaagcg ctgcggatga cgcggcacga tgacgagcag cggacgcatt
     2701 ccggcgagcg tccgccacgc ggccagcagc atggcctcct cgccgtcacg cgtgatcgcc
     2761 gcgaggacga cgggccgatc gcccgtcgcg gaccgccacg cgcgaccctc ggcgacgaga
     2821 tagggcgacg gcgtcaggtc gaacttc
//
LOCUS       DASRWS010000480         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1059023, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000480 DASRWS010000000
VERSION     DASRWS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059023"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="VFF72_09075"
     CDS             <1..1573
                     /locus_tag="VFF72_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HZV93357.1"
                     /translation="ERPLAILSDNDVEHLLLALGAMLAGVPFSPISPAYSLLSQDHGK
                     LRHIVGVLTPGLFYASGPEYAKAIAAAAPADAGIVLGEGTIDGRRSESFAQLAATRPT
                     PAVDAAHDAVGPDTIAKFLFTSGSTKLPKAVINTQRMLCSNQQMILQSFPGLGRTPPV
                     LVDWLPWNHTFGGNHNIGITLYNGGTLYIDEGKPTPALIGETLRNLREIAPTIYFNVP
                     KGFEEIANALEVDEQLRKTLLSRVTMFFFSGAGLSQPVWDKLEAVSVAHCGERIRMLT
                     GLGMTETAPFAICANAHQVKSGHIGLPAPGVEVKLVDDGGKREVRYRGPNVTPGYWRN
                     PEQTAASFDDEGFFMSGDAARPMDAADPGRGFVFDGRIAEDFKLATGTFVSVGPLRAR
                     IIAAGDPMVQDVVIAGINRNEIGILIFPRPDACRAYAGVDGAMPMREVLRQPKLRAFF
                     QRLVDVLHSQGTGSATRVARAVVLVEPPSIDKGEITDKGSINQRAVLTHRDADVVRLY
                     LGTGDDIILPAGKAA"
     gene            1570..2376
                     /locus_tag="VFF72_09080"
     CDS             1570..2376
                     /locus_tag="VFF72_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV93358.1"
                     /translation="MKIEGRTALVTGGASGLGAETARELARRGAKVAVLDRNGEGALA
                     VAAAIVGEHRAGRAIGIACDIVSTESVVAALDAATAALGAPRIVMNVAGIGTAKRIIG
                     KDGSPMPLEDFTRVVDVNLVGTFNVIRLAAARIAALQPLADAAGAEGTDERGLVVCTA
                     SVAAFDGQVGQEAYAASKGALVSLTLPLARDLAQHGIRVVTIAPGLFLTPLLAELPEP
                     VQASLAASIPFPKRLGRPAEFAHLACAIVENPALNGEVIRLDGALRMAPR"
     gene            2402..2821
                     /locus_tag="VFF72_09085"
     CDS             2402..2821
                     /locus_tag="VFF72_09085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZV93359.1"
                     /translation="MSRTTHHVVTVQFGDCDPAGIVFYPNFQRWMDESSLSFFMQCGL
                     PPWRELVKTRGIVGTPLLEIATRFVTAATYAETLDIETSVEEWRAKVFVHRHRVRRGE
                     TLICEGTETRAFVRRDPDNPDRLRAIPIPDDIKAMCA"
     gene            2923..4074
                     /locus_tag="VFF72_09090"
     CDS             2923..4074
                     /locus_tag="VFF72_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93360.1"
                     /translation="MTRFTRLFAGLALAFGAAGVAQADITMGVSLPLTGPTSALGIPC
                     KNGILLWPKTVAGEKLNVIILDDATDPTQGVKNARRFVTEDHVDLMIGSAATPIAVAM
                     APVAAESKTVQLALSPMPPLPGGKDAWTFRLPQSTAVMAIPIVDYWKKTGVKTFGFLG
                     YTDAYGESWLTDISAAAEKAGIKRVDVERFARPDTSVTGQALKLVQANPDAILIAASG
                     SGAAMPAKGLVERGYPKTKIFQTHGAASLDLIRVGGKDVEGSFVASGPATVGDLLPDS
                     NPSKKLAMQYAAEYDKAYGPKTANQFGGHVFDAYILLQHIVPQALKQGKPGTPEFRAA
                     LKTALETEGRVPVSQGVLDYTATDHFGFTPDTGILLTIENGNWKLVSTH"
     gene            4134..>4561
                     /locus_tag="VFF72_09095"
     CDS             4134..>4561
                     /locus_tag="VFF72_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93361.1"
                     /translation="MDATTAGILLLDGATNGAVYALLALAIVLVFAVTRVIFIPQGEF
                     VAYGALTLAMLQLGQVPQTIWLLVALAAIAAVLDLVDAVRRGLPPGTMLRGLLRTLLG
                     PVIVALLTWWAAPRHFPLYVQSALTLAIVTPLGPLVYRLA"
BASE COUNT          698 a         1662 c         1501 g          700 t
ORIGIN      
        1 cgaacggccg cttgcgatcc tgtccgacaa cgacgtcgag catctgctcc tcgccctggg
       61 cgccatgctg gccggagtgc ccttctcgcc gatctcgccg gcctattcgc tgctgtcgca
      121 agaccacggc aagctgcgcc acatcgtcgg cgtgctgaca cccgggctct tctatgccag
      181 cggcccggaa tacgccaagg cgatcgccgc ggcggcgccg gccgacgccg gcatcgtcct
      241 cggggaagga acgatcgacg gccgccgcag cgagtcgttc gcgcagctgg cggcgacgcg
      301 gccgacgccg gccgtcgatg cggcgcacga cgccgtcggc cccgacacga tcgccaagtt
      361 tctcttcacc tcgggctcga cgaagctgcc caaggccgtc atcaacaccc agcgcatgct
      421 ctgctcgaac cagcagatga tcctgcagag ctttccgggc ctcggccgga cgccgcccgt
      481 gctggtcgac tggctgccgt ggaaccacac cttcggcggc aatcacaaca tcggcatcac
      541 gctctacaac ggcggcacgc tctacatcga cgaaggcaag ccgacgccgg ccctcatcgg
      601 cgagacgctg cgcaacctgc gcgagatcgc gccgacgatc tacttcaacg tgcccaaggg
      661 tttcgaggag atcgccaacg ccctcgaggt cgacgagcaa ttgcgaaaga ccttgctgtc
      721 gcgcgtgacg atgttcttct tttccggcgc cggcctgtcg cagcccgtgt gggacaagct
      781 cgaggcggtg tcggtcgcgc attgcggcga gcggatcagg atgctcaccg gcctgggcat
      841 gaccgagacc gcgccgttcg cgatctgcgc caacgcgcat caggtgaagt cgggccacat
      901 cggcctgccg gcgcccggcg tcgaggtcaa gctcgtcgac gacggcggca agcgcgaggt
      961 gcgctatcgc ggcccgaacg tgacgccggg ctactggcgc aaccccgagc agacggcagc
     1021 gagcttcgac gacgagggct tcttcatgag cggcgacgcc gccaggccga tggacgcggc
     1081 cgaccccggt cgtggcttcg tcttcgacgg gcgcatcgcc gaggacttca agctcgccac
     1141 cggcaccttc gtctcggtcg gcccgctgcg ggcgcggatc atcgccgccg gcgatccgat
     1201 ggtgcaggac gtcgtcatcg ccggcatcaa tcgcaacgag atcggcatcc tgatctttcc
     1261 gcgacccgat gcctgtcgcg cctacgccgg cgtcgacggg gcgatgccga tgcgagaggt
     1321 gttgcgccag ccgaagctgc gggcattctt ccagcgcctg gtcgacgtgc tgcattcgca
     1381 gggcacgggc agcgcgacgc gcgtcgcccg ggccgtcgtc ctggtcgagc cgccgtcgat
     1441 cgacaagggc gagatcaccg acaagggctc gatcaaccag cgtgccgtgc tcacgcaccg
     1501 cgacgccgac gtcgttcgcc tgtatctcgg caccggcgac gacatcatcc ttccggcagg
     1561 gaaggccgca tgaagatcga agggcgcacc gcgctcgtca ccggcggtgc gtcggggctg
     1621 ggcgccgaga cggcgcgcga gctggcgcgg cgtggcgcca aggtggcggt gctcgaccgc
     1681 aacggcgagg gcgcgctggc ggtggccgcg gcgatcgtcg gcgagcaccg cgccggccgg
     1741 gcgatcggca tcgcctgcga catcgtctcg accgagagcg tcgtcgcggc gctcgacgcg
     1801 gcgacggcgg cgctcggcgc gccgcgcatc gtcatgaacg tcgccggcat cggcacggcg
     1861 aagcggatca tcggcaagga cggctcgccg atgccgctcg aggatttcac ccgcgtcgtc
     1921 gacgtcaacc tcgtcggcac gttcaacgtc atccggctcg cggcggcgcg gatcgcggcg
     1981 ctgcagccgc tggccgacgc ggccggcgcc gaaggcacgg acgagcgggg cctcgtcgtc
     2041 tgcaccgcgt cggtcgcggc gttcgacggc caggtcggcc aagaagccta tgcggcatcg
     2101 aagggcgccc tggtgtcgct gacgctgccg ctggcgcgcg atctcgccca gcacggcatc
     2161 cgcgtcgtca ccatcgcgcc cgggctcttc ctgacgccgc tgctggccga gttgcccgag
     2221 ccggtgcagg cgtcgctcgc cgcgagcatc ccgtttccga agcggctcgg ccggccggcc
     2281 gagttcgcgc acctggcctg cgccatcgtc gagaacccgg cgctcaacgg cgaggtgatc
     2341 cgcctcgacg gtgccctgcg catggcgccg cgctgagcgt gcaaaccggg ccgagtgatc
     2401 gatgagcagg accacccacc atgtcgtcac cgtccagttc ggcgactgcg atccggccgg
     2461 catcgtcttc tatccgaact tccagcgctg gatggacgaa tcgtcgctgt cgttcttcat
     2521 gcaatgcggc ctgccgccat ggcgagagct ggtcaagacg cgcggcatcg tcggcacgcc
     2581 gctgctcgag atcgcgaccc gcttcgtcac cgcagcgacg tatgccgaga cgctcgacat
     2641 cgagacctcg gtcgaggagt ggcgggccaa ggtcttcgtg caccggcacc gcgttcgtcg
     2701 cggcgagacg ctgatctgcg aaggcaccga gactcgcgcc ttcgtcaggc gagaccccga
     2761 caacccggat cgcctgcgcg cgattcccat tcccgacgac atcaaggcga tgtgcgcctg
     2821 agctgcgcac cggccctgat tcgcctcgcc gcctgtcgcg tcgaccgcgc ctcgaccccc
     2881 gatcttgatt ttcccgcccc catcgactcc caggagaact tcatgactcg attcacccga
     2941 ctgttcgccg gcctcgcgct ggccttcggc gccgccggcg tcgcccaggc cgacatcacc
     3001 atgggcgtca gcctgccgct caccgggccc acgtcggccc tgggcattcc ctgcaagaac
     3061 ggcatcctgc tctggccgaa gacggtcgcc ggcgagaagc tcaacgtcat catcctcgac
     3121 gacgcgacgg atccgacgca gggcgtcaag aatgcacgcc gcttcgtcac cgaggatcac
     3181 gtcgacctga tgatcggctc ggcggcgacg ccgatcgccg tggcgatggc gccggtcgcc
     3241 gccgagtcga agaccgtgca gctcgcgctt tcgccgatgc cgccgctgcc cggcggcaag
     3301 gacgcgtgga ccttccgcct gccgcaatcg acggcggtga tggcgatccc gatcgtcgac
     3361 tactggaaga agaccggcgt caagaccttc ggcttcctcg gctacaccga tgcctacggc
     3421 gaaagctggc tcaccgacat ctcggccgcc gccgagaagg ccgggatcaa gcgcgtcgac
     3481 gtcgagcgct tcgcgcgtcc cgacaccagt gtcaccggcc aagcgctcaa gctcgtgcag
     3541 gccaatcccg atgccatcct catcgccgcc tcgggctccg gcgcggcgat gcctgccaag
     3601 ggcctcgtcg agcgcggcta cccgaagacg aagatcttcc agacgcacgg cgcggcttcg
     3661 ctcgacctga tccgggtcgg cggcaaggac gtcgaaggct cgttcgtcgc ttccgggccg
     3721 gcgaccgtgg gcgatctgct tccagacagc aatcccagca agaagctggc gatgcaatac
     3781 gcggccgaat acgacaaggc ctacggcccg aagaccgcga accagttcgg cggccacgtc
     3841 ttcgatgcct acatcctgct gcagcacatc gttccgcagg cgctgaagca gggcaagccg
     3901 ggcacgcccg agttccgcgc cgccctgaag accgcgctcg agaccgaagg ccgcgtgccg
     3961 gtgtcgcagg gcgtgctcga ctacacggcg accgaccact tcggcttcac gcccgatacc
     4021 ggcatcctgc tgacgatcga gaacggcaac tggaagctcg tctcgacgca ttgagcgcac
     4081 gcgcgtccgc gtccaggccg cacagcccgc acacccgttg acgatcggcg cggatggacg
     4141 cgaccaccgc cggcatcctg ctcctggacg gcgccaccaa tggcgccgtc tacgccttgc
     4201 tggcgctggc catcgtcctg gtcttcgcgg tcacgcgggt catcttcatt ccgcagggcg
     4261 agttcgtcgc ctacggcgcg ctgacgctgg cgatgctgca gctcggccag gtcccgcaga
     4321 cgatctggct gctggtcgcg ctcgccgcca tcgccgcggt gctcgatctc gtcgacgcgg
     4381 tgcgccgcgg cttgccgccg ggaacgatgc tgcgcgggct gctgcgcacg ctgctcgggc
     4441 cggtgatcgt cgcccttctc acctggtggg cagcgccgcg ccactttccc ctctacgtgc
     4501 aaagtgcctt gacgctggcg atcgtcacgc cgctcgggcc gctcgtctat cgcctggcct
     4561 t
//
LOCUS       DASRWS010000481         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2773886, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000481 DASRWS010000000
VERSION     DASRWS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773886"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFF72_09100"
     CDS             <1..480
                     /locus_tag="VFF72_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV93362.1"
                     /translation="PDVMPLAKGLGSGVPIGAVVCGPRAADVMGPGNHGTTFGGNPLA
                     MRAGVETLRIMEEDRLLDNAASVGAALKAGLERELAGTAGVVEVRGQGLMIGIELDRP
                     CGGLLGRAADAGLMISVTADRVVRLLPALIMSRDEAAQIVAILCPLIREFLQAKPSQ"
     gene            477..1409
                     /gene="argF"
                     /locus_tag="VFF72_09105"
     CDS             477..1409
                     /gene="argF"
                     /locus_tag="VFF72_09105"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZV93363.1"
                     /translation="MKPGMNLIRHFLQFRDLRAEEYAYLFERAAEIKKRFKNYEKYTP
                     LVDRTLAMIFEKASTRTRVSFEAGMYQMGGSVVNLTSEGSQLGRDEPVEDTARVISRM
                     VDIVMIRTFEQAKLEAFAANSRVPVINGLTNEYHPCQILADIFTCIEQRGPMSGKIVA
                     WVGDGNNMATTWLQAADILDFTLHVATPNGYEIDPATVGLRRPDSLKVFGDPLDACRN
                     ADVVTTDVWTSMGFEDENEERRVAFADWRVDAEMMAAARPDALFMHCLPAHRGEEVDA
                     DVIDGPQSVVWDEAENRMHVQKALMEYLLLGRIG"
     gene            complement(1417..>2229)
                     /locus_tag="VFF72_09110"
     CDS             complement(1417..>2229)
                     /locus_tag="VFF72_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZV93364.1"
                     /translation="APITYIGVRYAVAFVVAMVAFGLRATWPRSRAQWGHIVFAGLLS
                     HAGYLAGSHYAQRWGLSAGVTALILSLQPLLTAVAVSRWLHVRLSKAQMLGVAIGLGG
                     VVLVVAHRIDGSALSLRALVAVVWALVCVTCGALYQRQFCADSDLRSAICIHFAATAA
                     VMLPLGAVVEGFAIVWNWQIAATLSYHVVLASIGAYSILHLLMRRGEATGVTSLIYLT
                     PPVAALSEWAVFGTPPSPTMWVGMGIACVGVAMVTRNRTTPAPKTPIIEEPS"
BASE COUNT          386 a          756 c          787 g          300 t
ORIGIN      
        1 cccgacgtga tgccgctggc caagggcctg ggatcgggcg tgccgatcgg cgcggtcgtc
       61 tgcggcccgc gcgccgccga cgtgatgggc cccggcaacc acggcaccac gttcggaggc
      121 aatccgctgg cgatgcgggc cggtgtcgag acgctgcgca tcatggaaga agaccggctg
      181 ctcgacaacg ccgccagcgt cggcgcggca ctcaaggccg ggctcgagcg cgagcttgcg
      241 ggaacggctg gcgtcgtcga ggtccgcggc cagggcctga tgatcggcat cgagctcgac
      301 cgcccgtgcg gcggtctgct cggccgcgcc gccgacgccg ggctgatgat cagcgtcacc
      361 gccgatcgcg tggtccggtt gttgcccgcg ctgatcatga gccgcgacga ggcggcgcag
      421 atcgtcgcca tcctctgccc attgatcagg gagttcctgc aagcgaagcc ttcgcaatga
      481 agcccgggat gaacctgatc aggcactttc tccagttccg cgatcttcgc gcagaggagt
      541 acgcctacct gttcgagcgc gcggccgaga tcaagaagcg cttcaagaac tacgagaagt
      601 acacgccgct ggtcgaccgg acgctggcga tgatcttcga gaaggcgtcg acgcgcaccc
      661 gggtcagctt cgaggcgggc atgtaccaga tggggggctc ggtggtgaac ctcaccagcg
      721 aaggcagcca gctcggccgc gacgaaccgg tcgaagacac ggcgcgcgtc atcagccgca
      781 tggtcgacat cgtcatgatc cgcaccttcg agcaggccaa gctcgaggcc ttcgcggcga
      841 actcgcgcgt gccggtgatc aacggcctca ccaacgagta ccacccgtgc cagatcctgg
      901 ccgacatctt cacctgcatc gagcagcgcg gcccgatgag cggcaagatc gtcgcctggg
      961 tgggcgatgg caacaacatg gcgacgacct ggctgcaggc ggccgacatc ctcgatttca
     1021 cgctgcacgt cgcgacgccg aacggctacg agatcgaccc ggcgacggtc ggcctgcgtc
     1081 gtcccgactc cctgaaggtc ttcggcgacc cgctcgacgc ctgccgcaac gccgacgtgg
     1141 tgacgaccga cgtctggacc agcatgggct tcgaggacga gaacgaggaa cgccgcgtgg
     1201 ccttcgccga ctggcgcgtc gacgccgaga tgatggcggc ggcgcgtccc gatgccctct
     1261 tcatgcactg cctgccggcg catcgcggcg aggaggtcga cgccgatgtc atcgacggcc
     1321 cgcagtcggt cgtctgggac gaggccgaga accgcatgca cgtgcagaag gcgctcatgg
     1381 agtacctgct gctcggccgc atcggctgag cgtcgcctac gaaggctcct cgatgatcgg
     1441 cgtcttcggc gccggcgtcg tgcggttcct cgtgaccatc gcgacgccca cgcaggcgat
     1501 ccccatgccg acccacatcg tcggcgacgg cggggtgccg aagacggccc attcgctcag
     1561 cgccgcgacg ggtggcgtga ggtagatcag gctggtcacg ccggtggcct cgccgcggcg
     1621 catcagcaga tgcaggatcg agtaggcacc gatcgacgcg agcacgacgt gataggacag
     1681 cgttgccgcg atctgccagt tccagacgat ggcaaagccc tcgacgacgg cgcccagcgg
     1741 cagcatgacc gcggccgtcg cggcgaagtg gatgcagatc gccgagcgca ggtcgctgtc
     1801 ggcgcagaac tggcgctggt agagcgcgcc gcaggtgacg cagacgagcg cccagacaac
     1861 ggcgaccagc gcgcgcaggc tgagggcgct gccgtcgatg cgatgggcga cgacgagcac
     1921 cacgccgccg aggccgatcg ccacgcccag catctgcgcc ttcgacaggc gcacgtgcag
     1981 ccagcgcgag acggcgacgg cggtgagcag cggctgcagc gacaggatca gcgcagtgac
     2041 gccggccgac aggccccagc gctgcgcgta gtggctgcca gccaggtagc cggcgtggct
     2101 gagcaggccg gcgaagacga tgtggcccca ctgggcgcgc gaccggggcc acgtcgcgcg
     2161 caggccgaac gcgaccatcg ccaccacgaa ggcgacggcg tagcgcacgc cgatgtaggt
     2221 gatcggcgc
//
LOCUS       DASRWS010000482         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_784378, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000482 DASRWS010000000
VERSION     DASRWS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784378"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /gene="rpoB"
                     /locus_tag="VFF72_09115"
     CDS             complement(<1..2094)
                     /gene="rpoB"
                     /locus_tag="VFF72_09115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZV93365.1"
                     /translation="MAQQAIPYSYTEKKRIRKSFGKRESVLGVPYLLTMQKDSYVAFL
                     QKDVPPQKRKPEGLQAAFLSAFPIVSHNGYVAMKFIEYNMAKPAFDTRECQQRGLTYA
                     AAVRAKLQMIIYDRESAQKDTVKEIKEQEVYMGEVPLMTDYGSFIVNGTERVIVSQLH
                     RSPGVFFEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDILYFRVDRRRKMPVTI
                     LLKAIGLNPEAILANFFVFDNFRLMDSGAQLEFVADRLRGEIARFDITDKDGKVIVEK
                     DKRITARHVRQIEQSETRFISVPEDYVVGRMLARNVVDAETGEILAKANDELTEAVLK
                     KLRLAGIKEIPALYTNELDEGAYISQTLASDETADQLAARVAIYRMMRPGEPPTEDAV
                     EALFHRLFYSADTYDLSRVGRMKFNARVGRDVPEGSMTLSNEDILDVVKILVELRNGR
                     GEVDDIDHLGNRRVRCVGELAENQYRSGLARIEKAVKERLGQAETEALMPHDLINSKP
                     ISAALKEFFGASQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHY
                     GRVCPIETPEGPNIGLINSLALYAQLNEYGFLETPYRRVADGKVTDKIDYLSAIEEGK
                     YVIAQASATLDTEGKLIDELVSARDNGESVLTSAERIQYMDVAPTQIVSVAASLVPFL
                     EHDDANRALMGANMQR"
     gene            complement(2500..2886)
                     /gene="rplL"
                     /locus_tag="VFF72_09120"
     CDS             complement(2500..2886)
                     /gene="rplL"
                     /locus_tag="VFF72_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZV93366.1"
                     /translation="MAFDKDAFISALDSMSVMELNDLVKAIEEKFGVSAAAMAAPGGG
                     GGGAGGAAAPAAEEQTEFTLVLSEIGANKVQVIKAVRELTGLGLKEAKDLVDGAPKPV
                     KEAMTKADAEAGKKKLEEAGAKAELK"
     gene            complement(2929..>3234)
                     /locus_tag="VFF72_09125"
     CDS             complement(2929..>3234)
                     /locus_tag="VFF72_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZV93367.1"
                     /translation="FSEDAVAAAKVLSDFAKTNDKLIVKAGAHAGKALDAKGVLSLAS
                     IPSKEVLLSQLLGLMQSPIARIARVLAVLAEKRGAESGAPAPAAPDAPVGDAAPAAA"
BASE COUNT          502 a         1051 c         1061 g          620 t
ORIGIN      
        1 gcgctgcatg ttcgcgccca tcagcgcccg gttcgcgtcg tcgtgctcga ggaacggcac
       61 cagcgacgcg gcgaccgaga cgatctgcgt cggcgcgacg tccatgtact ggatccgttc
      121 ggccgaggtc agcaccgact cgccgttgtc gcgcgccgag accagctcgt cgatgagctt
      181 gccctccgtg tcgagcgtcg cgctcgcctg cgcgatgacg tacttgcctt cctcgatcgc
      241 cgacaggtag tcgatcttgt cggtgacctt gccgtcggcg acgcggcgat acggcgtctc
      301 gaggaagccg tattcgttga gctgcgcgta gagggccagc gagttgatca ggccgatgtt
      361 cggtccttcc ggcgtctcga tcggacagac ccggccgtag tgcgtcggat gcacgtcgcg
      421 gacctcgaac ccggcgcgct cgcgcgtcag gccgcccggg cccagggccg agacgcgccg
      481 cttgtgcgtg atctccgaga gcgggttggt ctggtccatg aactggctca actgcgaggc
      541 gccgaagaat tccttcagcg ccgccgagat cggcttcgag ttgatcaggt cgtgcggcat
      601 cagggcttcc gtctcggcct ggccgagacg ttccttgacg gccttctcga tccgcgccag
      661 gcccgaccgg tactggtttt cagccagctc gccgacgcag cgcacccggc gattgccgag
      721 gtggtcgatg tcgtcgacct cgccgcggcc gtttctcagc tcgaccagga tcttgacgac
      781 gtcgaggatg tcctcgttcg acagggtcat cgagccttcc ggcacgtcgc ggccgacgcg
      841 ggcgttgaac ttcatgcggc cgacgcgcga gagatcgtag gtgtcggcgg aatagaagag
      901 gcgatggaag agtgcctcga cggcgtcttc ggtcggcggc tcgccgggcc gcatcatgcg
      961 gtagatggcc acgcgcgcgg cgagctggtc ggccgtctcg tcggacgcca gagtctgcga
     1021 gatgtaggcg ccctcgtcga gctcgttggt atagagcgcg ggaatttcct tgatgccggc
     1081 gaggcggagc tttttcagca ccgcttcggt cagctcgtcg ttggccttgg cgaggatctc
     1141 gccagtctcg gcatcgacga cgtttcgcgc cagcatgcgg ccgacgacgt agtcctcggg
     1201 caccgagatg aagcgcgtct ccgactgctc gatctggcgc acgtggcggg cggtgatccg
     1261 cttgtccttc tcgacgatga ccttgccgtc cttgtcggtg atgtcgaagc gcgcgatctc
     1321 gccgcgcagc cgatcagcga cgaactcgag ctgcgcgccc gagtccatca gccggaagtt
     1381 gtcgaagacg aagaagttgg cgaggatcgc ctcggggttg aggccgatcg ccttcagcaa
     1441 aatcgtcacc ggcatcttgc ggcggcggtc gacgcggaaa tagaggatgt ccttcgggtc
     1501 gaactcgaag tcgagccacg agccgcggta cgggatgatc cgcgcactga acagcagctt
     1561 gcccgagctg tgcgtcttgc ccttgtcgtg ctcgaagaac acgcccggcg agcggtgcag
     1621 ctgcgagacg atgacgcgct cggtgccatt gacgatgaac gagccgtagt cggtcatgag
     1681 cggcacttcg cccatgtaga cctcctgctc cttgatctcc ttgacggtgt ccttctgggc
     1741 cgactcgcgg tcgtagatga tcatctgcag cttggcccga accgccgcgg cgtaggtcag
     1801 gccgcgctgc tggcactcgc gcgtgtcgaa cgccggcttg gccatgttgt attcgatgaa
     1861 cttcatcgcc acgtagccgt tgtgcgagac gatcgggaac gcggagagga acgccgcctg
     1921 cagcccttcg ggctttctct tttgcggcgg gacgtccttt tgcaggaagg cgacgtagga
     1981 gtccttctgc atcgtcagca gataaggaac tcccaggacg ctctcgcgct tgccgaagct
     2041 ctttcggatg cgcttcttct cggtgtacga ataggggatt gcttgctgcg ccatgaactc
     2101 tccgttcaga accccgcggc cggccgtgac cgctgggccc acgaatgcac cgaacgaacg
     2161 gtgcattgcg aaggcgactg tctttcgggc cttgcgcccg aagcttggtg gcaggccact
     2221 acctgccgct ggcggacaac cccggcgttg caccggagcc cgaccaaacc cgtcccttgc
     2281 agtcggatca agggactttc gtcgaaatca cttggaagaa ccctcgattg tcgatcgggg
     2341 cgctttcagc tgctttcgaa aggtcaataa cacctcagca ggtaaatcgg acgccgggcg
     2401 tcgcgcggcc ccgaatctgg cgagagaagc gcggccgtac tcgagtacgg cgagcctcgc
     2461 aagcgccagg atcgggtcgc gcagcagccc ggggccgatt tacttgagct cggccttggc
     2521 gccggcttcc tccagcttct tcttgcccgc ttcggcgtcg gccttggtca tcgcttcctt
     2581 gacgggcttc ggcgcgccgt cgaccaaatc cttggcttcc ttcaggccga ggcccgtcag
     2641 ctcgcgaacg gccttgatga cctgcacctt gttggcgccg atctcggaca ggaccagcgt
     2701 gaactccgtc tgctcctcgg ccgccggcgc ggcggccccg cccgcaccac cgccgccacc
     2761 gcccggagca gccattgccg cggccgagac gccgaacttc tcttcgatcg ccttgaccag
     2821 gtcgttgagc tccatcaccg acatgctgtc gagagcggag atgaatgcgt ctttgtcgaa
     2881 tgccatttga gtttccagtt cagatattgg gttgcgaaat ccgctcgttc aggcggccgc
     2941 cggcgcggcg tcgccaaccg gcgcatcggg cgcggccggc gccggggcgc ctgactccgc
     3001 gccgcgcttt tcggccagca ccgccagcac gcgggcgatc cgcgcgatcg gcgactgcat
     3061 caggccgagc agctgcgaca gcagcacttc cttcgacggg atcgaggcga gcgagagcac
     3121 gcctttggcg tcgagggcct tgcccgcgtg ggcaccggcc ttgacgatca gcttgtcgtt
     3181 cgtcttggcg aagtcggaca ggactttcgc cgcggcgacc gcgtcctcgg aaaa
//
LOCUS       DASRWS010000483         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2466700, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000483 DASRWS010000000
VERSION     DASRWS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466700"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..717
                     /gene="dapB"
                     /locus_tag="VFF72_09130"
     CDS             49..717
                     /gene="dapB"
                     /locus_tag="VFF72_09130"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZV93368.1"
                     /translation="MAVAGAAGRMGETVCAAVEGADDMALVGRADPLLGVELGDVLGD
                     ADVVVDFTRPDTALENALSCLRAGVHAVVGTTGFDLQPLRDAAEAQQGDRANVLVAPN
                     FAIGAVLMMRFAAEAARHMHKAEIIELHHDRKLDAPSGTAALTAELMAQAGGGERPPI
                     HSVRLPGLVAHQEVLLGDLGQTLSIRHDSISRESFMPGVLLGVRRVDSLPEPLVVGLE
                     SLLF"
     gene            894..1799
                     /gene="dapA"
                     /locus_tag="VFF72_09135"
     CDS             894..1799
                     /gene="dapA"
                     /locus_tag="VFF72_09135"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZV93369.1"
                     /translation="MSDLGTILTAIVTPFDERSRVNEEAFVALMRHLGDNGSDGFVVA
                     GTTGEASTMTDEEQLGLIELAVAERPAGKTIVAGTGTNDTREAVFLTERATALGVDAT
                     LVVTPYYNKPSRLGITRHYETVAKATDKPMLVYNIPGRTATNMPVDLLAELAQIEHID
                     GVKQANADELQPIDGMALYAGDDATFARTLDLGGAGGICVASHIVGNEMRRMVDEPDR
                     RAEIDASLRDVYATLFMTSSPTCTKAALNLLGHEAGGLRLPLVEATEQELAEVRAMLT
                     RHGLLADDDGRAASAGASSAQARGA"
     gene            1850..>2082
                     /locus_tag="VFF72_09140"
     CDS             1850..>2082
                     /locus_tag="VFF72_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV93370.1"
                     /translation="MTVVEHDGRIVVVDVGLRFPTPEMVGIDLVLPDFSYLRERARDI
                     EAIVITHGHEDHLGALPWVLRELGQDAVPVVYGG"
BASE COUNT          306 a          758 c          716 g          302 t
ORIGIN      
        1 tgcgccggcc ccctccgccg cagcgcaggg ggcgtcgcga tgatcggcgt ggcggtcgcc
       61 ggcgccgccg ggcgcatggg cgagaccgtc tgcgccgccg tcgaaggagc cgacgacatg
      121 gcgctcgtcg gccgcgccga cccgctgctg ggcgtcgagc tcggcgacgt gctcggcgac
      181 gccgatgtcg tcgtcgactt cacgcgcccc gacaccgccc tggagaacgc gctgagctgc
      241 ctgcgcgcgg gcgtgcacgc cgtcgtcggc acgaccggct ttgacctgca gccgctgcgc
      301 gacgccgccg aggcgcagca gggcgatcgc gcaaacgtgc tcgtcgcgcc gaacttcgcc
      361 atcggcgccg tgctcatgat gcgcttcgcc gccgaggccg cgcgtcacat gcacaaggcc
      421 gagatcatcg agctgcacca cgaccgcaag ctcgacgcgc ccagcggcac cgccgcgctg
      481 accgccgagc tgatggcgca ggccggcggg ggggagcgcc cgccgattca ttccgtgcgc
      541 ctgcccggcc tcgtcgccca ccaggaggtg ctgctcggcg acctcggcca gacgctcagc
      601 attcgccacg acagcatcag ccgcgagtcg ttcatgccgg gcgtgctgtt gggcgtgcgg
      661 cgggtcgaca gtctgcccga accgcttgtg gtcgggctcg agagcttgct gttttgagca
      721 cgtccgatct ttcagcggcg gggggtagtt catgacctac ttgacggcat cctgcctttt
      781 gggtcgagcc ggcctgcact tctcgtcaca atttgtaacc gttccggccg ccacgacatg
      841 tcacctggac cggtcatccg gaccggtcga tggccgcgtg agatactgag gccatgtcag
      901 acctcggcac gattctcacg gcgatcgtca ctccgtttga cgagcgctcg cgcgtcaacg
      961 aggaggcgtt cgtggcgctg atgcgccatc tcggcgacaa cggctcggac ggcttcgtcg
     1021 tcgcgggcac gaccggcgag gcctctacga tgaccgacga ggagcagctg gggctgatcg
     1081 agctggcggt cgccgagcgc ccggcgggca agacgatcgt cgcaggcacc ggcaccaacg
     1141 acacgcgcga ggcggtgttc ttgaccgaac gcgcgaccgc gctcggcgtc gacgcgacgc
     1201 tcgtcgtgac gccctactac aacaagccca gccgcctggg catcacgcgc cactacgaaa
     1261 cggtcgccaa ggcgaccgac aagccgatgc tcgtctacaa catccccggg cgcacggcca
     1321 cgaacatgcc cgtcgacctg ctcgccgagc tcgcgcagat cgagcacatc gacggcgtca
     1381 agcaggccaa cgccgacgag ctgcagccga tcgacggcat ggcgctgtac gccggcgacg
     1441 acgcgacctt cgcgcgcacg ctcgatctcg gcggcgccgg cggcatctgc gtggccagcc
     1501 acatcgtcgg caacgagatg cgccggatgg tcgacgagcc cgacaggcgc gccgagatcg
     1561 acgcctccct gcgcgacgtc tacgcgacgc tgttcatgac ctccagcccc acctgcacga
     1621 aggccgcgct gaacctgctc ggccacgagg ccggcggcct gcgcctgccg ctcgtggagg
     1681 ccaccgagca ggagcttgcc gaggtgcgcg cgatgctcac ccgtcatggc ctgctcgcgg
     1741 acgacgacgg gcgggccgcg tcggccggcg cgtcctcggc tcaggcccgc ggcgcgtgag
     1801 ctccgttcgt gtcctgcccc tcggcggcct gggggagatc ggcaagaaca tgaccgtcgt
     1861 cgagcacgac gggcggatcg tcgtcgtgga cgtcgggctg cgcttcccga cgcccgagat
     1921 ggtcggcatc gacctcgtcc tgcccgattt ctcctatctg cgcgagcgcg cgcgggacat
     1981 cgaggcgatc gtgatcacgc acggccacga ggaccacctc ggcgcgctgc cgtgggtcct
     2041 gcgcgagctc ggccaggacg cggtccccgt cgtctacggc gg
//
LOCUS       DASRWS010000484         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1647726, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000484 DASRWS010000000
VERSION     DASRWS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647726"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="VFF72_09145"
     CDS             complement(<1..2213)
                     /locus_tag="VFF72_09145"
                     /inference="COORDINATES: protein motif:HMM:NF023400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZV93371.1"
                     /translation="MPAAYAASVVAVSPSGEVAKVREVTVKFSEAVVPFGDPRVADPF
                     SIACQGTPPTGAGRWASDRVWLYDFREDLPPGTRCTVKALVDWKPTPKGGASSSSLTG
                     PTEFTFSTGGPAVVSAQPSAGSDIDADAYFLLRLSGPAVESTVGEHAWCEAEGIGERL
                     PVQVVGGDVREQVLKARRIDKARAANMLLVHCTRPLPDDAAARLVWGKGIAAAANPAV
                     VTTIEQRFRYHVRPAFTADFTCERENASAPCLPIRPMSVRFSAPVARALAAQVQLVPA
                     AGGAALAPVFDKDDKSTEVSEIAFPKPLAENAAYRIVMPPGLHDDAGRALANAASFPL
                     KVATGAAPPIAKFAAAPFGIIERDADAALPVTLRHVQVDLGSGNPAAGASPAGSTGRI
                     RDKLLTSDSDILSWYALVQKYHETELSARELGFPQSTWSTTEEVTDAKGRTSRRKVDR
                     MVGTREVSLLAREPDVRSLTLPQLGAGDARPFEVIGIPLPQPGYHVVEIESLRLGQSL
                     LDKRAPMFVRTGVLVTNLGVHFKLGRENSLVWVTSLDRGKPVEGADVAVNDCFGKPLW
                     NGRTDAKGLAVVARALDASFDHCPADGGYFVTAHRRGDVAFVFSTWQKGIESWRFNVP
                     TARGAEPDLRASTVFDRTLFRAGETVSMKHFIRVETSRGLAQVAADRLPTRVKIVHEG
                     SGQEFVFPLQWLAGGRSATTTWKIPPAAKLGVYDVSLEREGGGGGGDETHSRSSG"
BASE COUNT          389 a          853 c          861 g          373 t
ORIGIN      
        1 ctgccgctcg accggctgtg cgtctcgtcg ccgccgccgc caccgccctc gcgctcgagc
       61 gagacgtcgt agacgccgag cttggccgcc ggcgggatct tccaggtcgt cgtcgcgctg
      121 cggccgccgg cgagccactg cagcggaaag acgaactcct ggccgctgcc ttcgtggacg
      181 atcttcaccc tggtcggcag acggtccgcg gccacctgcg ccaggccgcg cgaggtctcg
      241 acgcggatga agtgcttcat cgacaccgtc tcgccggcgc gaaagagcgt ccggtcgaac
      301 accgtcgagg cgcgcaggtc gggctcggcg ccacgggccg tgggcacgtt gaagcgccac
      361 gattcgatgc ccttttgcca ggtgctgaag acgaaggcga catcgccccg gcgatgcgcg
      421 gtgacgaagt agccgccatc ggccggacag tgatcgaagc tcgcgtcgag agcgcgtgcg
      481 acgaccgcca ggcccttcgc gtcggtgcgg ccgttccaga gcggcttgcc gaagcagtcg
      541 ttgacggcga catcggcgcc ttcgacgggc ttgccccgat cgagcgacgt cacccagacg
      601 aggctgttct cgcggccgag cttgaagtgg acgccgaggt tggtgacgag cacgccggtg
      661 cgcacgaaca tcggtgcgcg cttgtcgagc agcgactggc cgagccgcag cgactcgatc
      721 tcgacgacgt gatagcccgg ctgcggcagc ggaatgccga tcacctcgaa cggtcgcgca
      781 tcgccggcgc cgagttgcgg cagcgtcaaa gaccggacat cgggctccct ggcgagaagc
      841 gagacctcgc gcgtgccgac catccggtcg accttgcgcc ggctcgtccg gcccttcgcg
      901 tcggtgactt cctcggtcgt cgaccacgtg ctctgcggaa aaccgagctc cctggctgag
      961 agctcggtct cgtgatactt ctgcaccagc gcataccagg acaggatgtc ggagtcgctg
     1021 gtcaggagct tgtcgcgaat gcggccggtc gatcccgcgg gcgaggcgcc ggcggccggg
     1081 ttgccgctgc cgagatcgac ctggacatgg cgaagggtga ccggcagcgc ggcatcggcg
     1141 tcgcgctcga tgatgccgaa aggcgccgcc gcgaacttgg cgatcggcgg cgccgcgccg
     1201 gtcgcgacct tgagcggaaa actcgccgcg ttggcgagcg cgcgtccggc gtcgtcgtgc
     1261 aggccgggcg gcatgacgat gcgataggcg gcgttctccg ccagcggctt cggaaaggcg
     1321 atctcgctca cctcggtcga cttgtcgtcc ttgtcgaaca ccggcgccag cgccgcgccg
     1381 ccggcggccg gcacgagctg cacctgggcg gcgagcgccc gcgccaccgg cgccgagaag
     1441 cgcacgctca tcggccggat cggcaggcag ggcgccgacg cgttctcgcg ctcgcacgtg
     1501 aaatcggccg tgaacgccgg acgcacgtga tagcgaaagc gctgctcgat cgtcgtcacg
     1561 accgccgggt tggccgccgc ggcgatgccc ttgccccaga cgagcctcgc cgccgcatcg
     1621 tcgggcagcg gccgcgtgca atgaacgagc agcatgttcg ccgcgcgtgc cttgtcgatg
     1681 cggcgcgcct tcagcacctg ctcgcgcacg tcgcctccca ccacctgcac cggcagtcgc
     1741 tcgccgatgc cttcggcctc gcaccaggcg tgctcgccga ccgtcgactc gacggccggc
     1801 ccgctcaggc gaaggaggaa gtacgcatcg gcgtcgatgt cgctgcctgc cgagggctgc
     1861 gccgagacga cggccgggcc gccggtggaa aaggtgaatt cggtcggccc tgtcaacgag
     1921 ctgctgcttg cgccaccttt aggggtcggc ttccaatcga cgagtgcctt gaccgtgcaa
     1981 cgcgtccccg gcggcaagtc ctctcgaaag tcgtagagcc acacccgatc gctcgcccag
     2041 cggccggcgc ctgtcggagg ggtgccctgg caggcaatgg agaacggatc ggcaacgcgc
     2101 gggtcgccga aggggacgac agcttcggaa aatttgaccg tgacctcgcg gaccttcgcc
     2161 acctcgccgc tcgggctaac ggcgacgacg ctcgccgcgt aggccgccgg catgagtgcg
     2221 agcaagcttg cagttacgaa acaacgcacg tctttcactc tgggctcctg gcgattgcgc
     2281 ggcagtctac gggccgaaaa gagcggcagg aagaagagag gatggaggtc gtgagaagag
     2341 accgaatgcg catgcgaaac cggctcgtcg cagtcgaccc gccaatgagc cccaagcgtg
     2401 tgtcgctttg cactctttcc gccatcccca cgggggcgtt acgccggtac actgagtcgt
     2461 gtctttcgcg cgtcgg
//
LOCUS       DASRWS010000485         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4217149, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000485 DASRWS010000000
VERSION     DASRWS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217149"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="VFF72_09150"
     CDS             complement(<1..1744)
                     /locus_tag="VFF72_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZV93372.1"
                     /translation="MTTIRYEIDGSGIATVTFDAVGAPVNTMTREWQADLADVSRRLV
                     ADRPNIRGVVLASAKSTFFAGAALGHVLTLKPEDAAPGFAEIEAIKASYRRIETLGRP
                     VVALLNGAALGGGWELALAAHARFAIDDASISFGLPEVTLGLIPGATGITKTVRLLGL
                     AAAQPYLVEGRLFGPREGLELGLVDGVADDLDALKALALAWIDAHPDAQQPWDRRDYR
                     MPGGAPSSPKLAAALSVAPAMLLQKTRGLYPATEAILETMAEGALVDYDTATRIESRK
                     LARIMVGQTARNMIQAFFFDLNAIRSGKSRPAGFARWRPTRVGILGAGMMGAGIAHAN
                     AARGIACVLTDVSIERANAGVESIRRITAAQVARGRLELAGEARLLGLVTPSADPAAL
                     DGCELIIEAVFENREMKAAVTRQAWPMLAPGGVFASNTSTLPIGGLAEASADPTRFVG
                     LHFFSPVNRMRLVEIIRGARTSDETIARAIDYVAALGKTPIVVNDSRGFFTSRVFASF
                     VMEGVSMLAEGIGAPLIEHAAQAAGMPVGPLAVLDETSLSLSVHVIEQTRADLAAEGK
                     TYVPSSAEQLVERMV"
     gene            complement(1801..>2381)
                     /locus_tag="VFF72_09155"
     CDS             complement(1801..>2381)
                     /locus_tag="VFF72_09155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462498.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZV93373.1"
                     /translation="PRTTLEGLASLKVAFAELGAMGFDAVALARYPQVERIEHVHTAG
                     NSSGIVDGAAAVLVGSEAKGRAMGLAPRGRIVATALSGADPTIMLTGPMPATRKVLAK
                     AGLAIDDIDLFEVNEAFAAVPMRFMREMGVPEEKVNVNGGAIAMGHPLGATGAMLVGT
                     LLDELERRKLRRGLVTLCVGGGMGIATIVERV"
BASE COUNT          345 a          823 c          865 g          348 t
ORIGIN      
        1 gcaccatgcg ttcgaccagc tgttcggcgg acgacggcac ataggtcttc ccttcggcag
       61 cgagatcggc gcgcgtctgt tcgatgacgt gcaccgacag cgacagcgag gtctcgtcga
      121 gcaccgcgag cggcccgacc ggcatgccgg cggcctgcgc cgcatgctcg atcagcggcg
      181 cgccgatgcc ttcggcgagc atcgacacgc cttccatgac gaagctggcg aagacgcggc
      241 tggtgaagaa gccgcgcgaa tcgttgacga cgatgggcgt cttgccgagt gccgcgacgt
      301 aatcgatggc gcgggcgatc gtctcgtcgc tggttcgggc gccgcggatg atctcgacca
      361 ggcgcatccg gttcaccggc gagaagaagt gcaggccgac gaagcgggtc gggtcggcgc
      421 tcgcctcggc aagtccgccg atcggcaggg tcgaggtatt gctggcgaag acgccgccgg
      481 gcgccagcat cggccaggcc tgccgggtca ccgccgcctt catctcgcgg ttctcgaaga
      541 ccgcctcgat gatcagctcg cagccgtcga gtgccgccgg atcggcgctc ggcgtgacga
      601 gaccgagcag gcgggcctcg ccggctagct cgagccggcc cctggcgacc tgcgcggcag
      661 tgatgcggcg gatcgactcg acgccggcat tcgcccgctc gatcgacaca tccgtgagga
      721 cgcaggcgat gccgcgcgcg gcattggcgt gggcgatacc ggcgcccatc atgccggcgc
      781 cgaggatgcc gacccgcgtc ggtcgccagc gcgcgaagcc ggccggccgc gacttgcccg
      841 agcggatcgc gttgaggtcg aagaagaacg cctggatcat gttcctcgcc gtctgcccga
      901 ccatgatccg ggcgagcttt cggctctcga tgcgggtcgc ggtgtcgtag tcgacgaggg
      961 cgccttcggc catcgtctcg agaatcgctt cggtggccgg gtagaggcca cgcgtctttt
     1021 gcagcagcat ggccggcgcg acgctcagcg ccgcggcgag cttcgggctc gatggcgcgc
     1081 cgcccggcat ccggtagtcg cgacggtccc acggctgctg cgcatcggga tgggcgtcga
     1141 tccaggccag ggccagcgcc ttcagcgcgt cgaggtcgtc ggcgacgccg tcgacgaggc
     1201 cgagctcgag gccctcgcgc gggccgaaga gcctgccctc gacgagatac ggctgggcgg
     1261 ccgccagtcc gagcagacgc acggtcttcg tgatgccggt cgcgccggga atcaggccga
     1321 gcgtcacctc gggcagcccg aacgagatcg atgcgtcgtc gatcgcgaag cgggcatggg
     1381 cggccagggc cagctcccag ccaccgccga gcgcggcgcc gttgagcagc gcgacgaccg
     1441 ggcggccgag cgtctcgatg cggcggtagc tcgccttgat cgcctcgatc tcggcaaagc
     1501 cgggcgcggc gtcttcgggc ttgagcgtca gcacatggcc gagggcggcg ccggcaaaga
     1561 acgtcgactt ggccgaagcg aggacgacgc cgcggatgtt cggccgatcg gcgaccaggc
     1621 gtcgcgacac gtcggcgagg tcggcctgcc actcccgcgt catcgtgttg accggcgcgc
     1681 cgacggcgtc gaaggtgacg gtggcgatgc cgctgccgtc gatctcgtag cggatcgtcg
     1741 tcatcgcggc cgaggtgtcg atgtcatggc atccgatgtc tcgagagatc gagccacgcc
     1801 tcagacgcgc tcgacgatgg tcgcgatgcc catgccgccg ccgacgcaca gcgtcacgag
     1861 gccgcgcctg agctttcgcc gctcgagctc gtcgagcagc gtgccgacga gcatcgcccc
     1921 cgtggcgccg agcggatgcc ccatggcgat ggcgccgccg ttgacgttga ccttctcctc
     1981 gggcacgccc atctcgcgca tgaagcgcat cggcaccgcg gcgaaggctt cgttgacctc
     2041 gaagaggtcg atgtcgtcga tcgcgagtcc cgccttggcg aggaccttgc gcgtcgccgg
     2101 catcgggccg gtgagcatga tggtcgggtc ggccccggag agcgcggtgg cgacgatgcg
     2161 gccgcgcggc gccaggccca tcgcgcggcc cttcgcttcg cttccgacca gcaccgcggc
     2221 ggcgccgtcg acgatgcccg acgagttgcc cgccgtgtgc acgtgctcga tgcgctcgac
     2281 ctgcggatag cgggccagcg ccacggcgtc gaagcccatc gccccgagct cggcgaaagc
     2341 gaccttcagc gacgccaggc cctcgagcgt ggtgcgcggc t
//
LOCUS       DASRWS010000486         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_488862, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000486 DASRWS010000000
VERSION     DASRWS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488862"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1861
                     /locus_tag="VFF72_09160"
     CDS             182..1861
                     /locus_tag="VFF72_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZV93374.1"
                     /translation="MIHLLNLLAAIALLVWGTHIVRTGMLRVFGENLRRILATSFSNR
                     FRAFIAGLGVTSLVQSSTATCLIVASFVGKNLVGTSAALAVMLGADVGTSMMTVVFSL
                     DLSWLSPLLIFTGVVVFISKQNSGAGAVGRVLIGLGVITLALQLIVAATRPLTDAPAI
                     RNLLVSLPSDVFLDIVVGTVLTVLSYSSLAIVLLIATLAASSLIPITAALGLVLGANI
                     GSGILAMLATANASAQVRRLPLGNLIFKVIGTLFFFGFLPEVHVLLDQLVMSVHQQVV
                     VFHLVFNVVMAVALIGFTGLIGRTVERWLPEPTPGADLLRPRHLDPLALNMPSLAISC
                     AAREALHQADVVETMLRGIPLVLKNNDLRLSGELRKLDDTVDELYSAIKFYLTQISGE
                     ALSKEESRRWTDIVSFTINMEQIGDIIERVLQDIEDKKVKKSRSFSDAGMAEIIHLHE
                     RLLANLRLAMSVFLDGHVRDAKRLLEEKAHFRDLEHEYAANHIGRLRDNTTQSIETSS
                     LHLDLISDLKRINSHICSIAYPILESAGALTATRIRQSQFAALESPKETAP"
     gene            1920..2783
                     /locus_tag="VFF72_09165"
     CDS             1920..2783
                     /locus_tag="VFF72_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZV93375.1"
                     /translation="MATLTWPIIGAVLFAALLHASWNALIKSGDDKALDTALIHLLGC
                     AVGLALVATVGLPRRDALPWLFASAAVHLGYYVTLVGAYRHGELGFSYPIMRGTAPLL
                     VALLSGRLVGEHLPAVGWLGVAGISGGVLLTGLSRVPRESGSRRHALGFALANACIIA
                     TYTLIDGTGVRISGDALPYVGLLYLINGVPYFGLVMWQRREALAPAAAYMRRRWPAAL
                     LGACASLGAYGIALWAMRQAPLASVAALRETSVLFAAVIGLLLLGERFRLQRMLGAGA
                     IVAGVMALRLA"
     gene            complement(2797..>3240)
                     /locus_tag="VFF72_09170"
     CDS             complement(2797..>3240)
                     /locus_tag="VFF72_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93376.1"
                     /translation="RRPAMSDIAVENYKKGVANALDRWAKKVKPVAEQIDKLNSVLKK
                     LLENKSPSAEDKKKIDDCKAARDKLQKKIETFDLELKTDLMLLDMPQKGKADEKEIVK
                     LPKWIEEIVKKKGVSIGTVAVAPDIDIDFKAGKLKKVGLIIKFTF"
BASE COUNT          465 a         1146 c         1002 g          627 t
ORIGIN      
        1 tgacggcgtc gacgcgcggc ttcgctcgca cggcgaaagc gccgggcaga aggtgcccct
       61 cgtcgtcaat cgacgtctgc acggaatcga tccggcacgc cgcttccgca acttcgacgc
      121 cttcgatctg gtcctcaacc atgtcgactg attcgcttca tcccggggag ctcggcgcgc
      181 attgatccat cttctcaatc ttcttgccgc gatcgcgctc ctcgtctggg gcacgcacat
      241 cgtccgcacc ggcatgctgc gcgtcttcgg ggagaacctg cgccgcatcc tggcaacgag
      301 cttttcgaac cgctttcgcg ccttcatcgc cgggctcggc gtcaccagcc tggtccagtc
      361 gagcacagcc acctgcctga tcgtcgcctc gttcgtcggc aagaacctcg tcggcaccag
      421 cgccgccctc gccgtcatgc tcggcgccga tgtcggcacg agcatgatga cggtggtctt
      481 ctcgctcgac ctttcgtggc tgtcgccgct gctcatcttc acgggcgtcg tcgtcttcat
      541 ctcgaagcag aacagcggcg ccggtgcggt cggccgggtc ctcatcggcc tgggcgtcat
      601 caccctcgcc ctgcagctga tcgtcgccgc cacgcggccg ctcaccgacg caccggcgat
      661 tcgcaacctg ctcgtctcgc tgccgagcga cgtgttcctc gacatcgtcg tcggtaccgt
      721 gctgacggtg ctttcctact cgagcctggc gatcgtcctg ctcatcgcca cgctggcggc
      781 gtcctcgctg atcccgatca ccgctgcgct cggcctggtg ctgggcgcca acatcggcag
      841 cggcatcctg gcgatgctgg ccaccgccaa cgcctcggcg caagtgcgca ggttgccgct
      901 cggcaacctg atcttcaagg tgatcggcac gctcttcttc ttcggcttcc tgcccgaagt
      961 gcacgtcctg ctcgaccaac tggtgatgtc ggtgcaccag caggtcgtgg tctttcatct
     1021 ggtcttcaac gtcgtcatgg cggtggcgct gatcggcttc accggcctga tcgggcgaac
     1081 cgtcgagagg tggctgcccg agccgacgcc gggcgccgac ctgctgcgtc ctcgccatct
     1141 cgatcccctg gcgctcaaca tgccatcgct ggcgatctcg tgcgccgcac gcgaggcgct
     1201 gcatcaggcc gacgtcgtcg agacgatgct gcgcggcatc ccgctcgtgc tgaagaacaa
     1261 cgacctgcgc ctgtccggcg aattgcgcaa gctcgacgac acggtcgacg agctctactc
     1321 ggccatcaag ttctacctga cgcagatctc gggcgaggcc ctgtcgaagg aagagagccg
     1381 gcgctggacc gacatcgtct cgttcaccat caacatggag cagatcggcg acatcatcga
     1441 gcgcgtgctg caggacatcg aagacaagaa ggtgaagaag tcgcgcagct tttccgatgc
     1501 cgggatggcc gagatcatcc acctgcacga acgcctgctc gccaacctgc gcctggcgat
     1561 gagcgtcttt ctcgacggcc acgtccgcga cgcgaagcga ctgctcgagg aaaaggcgca
     1621 ctttcgcgac ctggagcacg aatacgcggc caaccacatc ggccgcttgc gcgacaacac
     1681 gacgcaaagc atcgaaacca gctcgctgca cctcgacctc atcagcgatc tgaagcgcat
     1741 caattcgcat atctgctcga tcgcctatcc gatcctcgag tcggccggcg cgctgacggc
     1801 gacacgcatc cgccagtcgc agttcgcggc gctcgagagc cccaaggaaa cagcgccctg
     1861 aggcacggac cggtccacgc ggccccaggc gcgaacgccg gcgcagccgc acggcgctga
     1921 tggcgacgct gacctggccg atcatcggcg cggtgctgtt cgccgcgctg ctgcacgcgt
     1981 cctggaacgc gctcatcaag tcgggcgacg acaaggcgct cgacacggcc ctgatccacc
     2041 tcctgggctg cgcagtcggc ctggcgctgg tcgccacggt cggcctgccc cgccgcgacg
     2101 cgctgccgtg gcttttcgcc tcggccgccg ttcacctggg ctactacgtc acgctggtgg
     2161 gtgcctaccg gcacggcgag ctcggctttt cctacccgat catgcgcggc acggcgccgc
     2221 tgctggtcgc cttgctcagc ggcaggctgg tcggagaaca tctgcccgcg gtcggctggc
     2281 tcggcgtggc cggcatcagc ggcggcgtgc tgctgaccgg cctgtcgcgc gtgccccggg
     2341 agtcagggtc ccggcgccac gcgctcggct tcgcgctcgc caacgcctgc atcatcgcca
     2401 cctacacctt gatcgacggc accggcgtgc ggatctcggg cgacgccctg ccctacgtcg
     2461 gcctgctcta tctgatcaac ggagtgccgt acttcgggct cgtgatgtgg caaaggcgcg
     2521 aggcactcgc gcccgcggcg gcctacatgc gtcggcgctg gccggcggcc ctgctcggcg
     2581 cctgcgcatc gctcggtgcg tacggcatcg cgttgtgggc gatgcgccag gcaccgctcg
     2641 cctccgtggc ggcgctgcgc gagacatcgg tgctgttcgc ggccgtgatc ggacttctgc
     2701 ttctcggcga gagattccgc ctgcagcgca tgctgggcgc cggcgcgatc gtcgccggcg
     2761 tgatggcgct gcggctggcc tgaacggccg gaccgtctag aacgtgaact tgatgatgag
     2821 gccgaccttc ttcagcttgc ccgccttgaa gtcgatgtcg atgtcggggg cgaccgcgac
     2881 cgtgccgatg ctcacgccct tcttcttcac gatctcctcg atccatttgg gcagcttgac
     2941 gatctccttc tcgtcggcct tgcctttctg gggcatgtcg agaagcatga ggtcggtctt
     3001 cagctcgagg tcgaaggtct cgatcttctt ctgcagcttg tcgcgggccg ccttgcagtc
     3061 atcgatcttc ttcttgtcct cggcgctcgg gctcttgttc tcgagcagct tcttgagcac
     3121 cgagttcagc ttgtcgatct gctcggccac cggcttgacc ttcttggccc agcgatcgag
     3181 ggcgttggcg acgcccttct tgtagttctc gacggcgatg tccgacatgg caggcctcct
//
LOCUS       DASRWS010000487         6395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_785240, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000487 DASRWS010000000
VERSION     DASRWS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785240"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1210
                     /gene="galE"
                     /locus_tag="VFF72_09175"
     CDS             197..1210
                     /gene="galE"
                     /locus_tag="VFF72_09175"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZV93377.1"
                     /translation="MILLTGATGYIASHTWLALHSAGLRVVGLDNFANSSPEVLERLA
                     ALGVDARAFVRADVTDRAALDEVFRRWPIEAAIHFAALKSVGESVARPLEYHRVNVGG
                     LVALLGAMQAAACRTFVFSSSATVYGEPERLPLAEDARLAATSPYGATKLIGEDVLRD
                     LGRSEAGWRVGVLRYFNPVGAHPSGTIGEDPRGTPNNLMPYIAQVAVRRRPRLQVFGA
                     DYPTPDGTGVRDYLHVDDLAEGHVAALRHLSSGAPSFTVNLGTGRGVSVLELVRAFER
                     SSGQPVPFEIVGRRPGDVAACYADPARACSLLGWRATRDLDAMCADTWRWQQANPDGF
                     AAR"
     gene            1214..1774
                     /gene="rfbC"
                     /locus_tag="VFF72_09180"
     CDS             1214..1774
                     /gene="rfbC"
                     /locus_tag="VFF72_09180"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZV93378.1"
                     /translation="MKITTTAIDGVLVIEPRVFGDPRGFFLESFNARDFAGATGRHVE
                     FVQDNQSRSTRGVLRGLHWQEAPHAQGKLVRVVSGAVFDVAVDIRRASPTFGRWIGVE
                     LSGDNHCQIWIEPGLAHGFLVLSETADVLYKTTTYYAPAAERSIRFDDPDLAIAWPDV
                     GMAPTVSAKDTAGMSFAAWRAEASAR"
     gene            complement(1852..3222)
                     /locus_tag="VFF72_09185"
     CDS             complement(1852..3222)
                     /locus_tag="VFF72_09185"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HZV93379.1"
                     /translation="MKGLRRRPLLQAAAVAAAGAIVWPGPARAQAKAGYAVLVGNTSY
                     NPAAENLPPAQKCLRDIEPQLRRLGFDVVALHDAPVSTVQAEIVKMQQAVAANPGMPA
                     VFYFVGHGFQSNAENFMVPAGSDLDAVPAQLSKTCISPEKDIFSHLRRLDGPAATVIM
                     IDACRTPDRPRSPGEGYNQTMPPEGCHVAFATGPGKRAFAPNDPQRDTLFAEVLVAEL
                     TASPPDRSILLTLESVRAKVANKVNSIPTIVKAFGPNAQQPELASNVSGDPPWDPALA
                     SKPAPTDGARPPAPASSAASELAAARAAATPEEAAAKLRDLLQKTPEGDDADVARLRL
                     KDLETVVAAARAARLDLDPAKLTAGQPPRVVEDMRRALQGDKYAALRVAETLPQPGAG
                     GELIERTDYGRWMIFAARLGNGIAAYRLSLYFRNVDRRDVEASRYLSLARANNYTPPR
                     QLETGR"
     gene            complement(3233..>6395)
                     /locus_tag="VFF72_09190"
     CDS             complement(3233..>6395)
                     /locus_tag="VFF72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF037374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HZV93380.1"
                     /translation="VVWADNQTSYAGQIAARGGAQGGDGGQAEVSGKKLLDFQGDVDL
                     RAVKGRTGQLLLDPQSLVIGTVADVNGDGTSGDDLAGNVLNASDYPSANSQITAARVA
                     SLLNTTDVTLASSISLSVTAPLTVAAGGAATTLSLTSGNVYLNAAMTLNNSSLSAQTT
                     PITSPSVEVNAAVTSLATVSLSGGAQVNVNSGGSLNAGTVNLTAPTVNVNGAINATTM
                     TLTGTTINLNAPTTATTMSLLANNDSATTTIQQDSLGAGIVSAGTLTVDGASGFNPPG
                     GVDLSHANNQIGRLVVNAGDAQFNVANAPGVPLVLSGTASDLAVTGVNTSIAQPAGAL
                     GVLSIGAGEGFFHVTTTGSGDITLNNPGNSLFGVTFNAAGNVNIAAGGNLDASGSATG
                     NVTLAAIGGAFYLDDTIAAANVDMSGVGFVNNTSYVNPPFTLPVGGRFTIHSSDYTQD
                     NFGTLGFGVASSSINYIVYGGYTGAAPGTGNGLFTNQTGSLAPPVTDNPPISKVYDGT
                     TNFNYTQTGTTSAGTLNAATPVALTLSNYTVNGTGNFVDKNAGANKQLTVGPSNDTSA
                     VAPSDNGATLYGLQFAGYTRAAGPHVAGTPGNAVSQITPKPITASGIDGIDRVYDGTT
                     GVGLDAAGASLTGVVAGDTVNLATTSAVGTLANKNIGVNKAVSITGLALTGGDQGNYT
                     VSDASGATATISARSITSTGFAGVGRVYDGTTAVAVNGSGASLGGVIAGDTVGIAGGA
                     TGTLADKNVGAAKPVTITGVALNGADAGNYTVTDASGATATITPRPLTSTGITGVDRT
                     YDATTVVAVSTAGATLNNAVAGDNVSIVGAGASGTIADKNVGTAKPVTVSGLALGGGD
                     AGNYTLTDASNATVNITPLAVQATGITAVNRADDGSTAVGLNTAGAGVNGVLPGDTVG
                     VDASGAVGSVATPAPGEAKPVTVTGIVLTGPDAIDYSVLPTPIAPGGGPLTVRILSVA
                     QSRFDDIRFKEYLQGVADAQEPFRRAMLEALLSGFGKENIRKQLQRGLVYETGLAPPA
                     VDIIDSAKPPASCDATAGLRCGQ"
BASE COUNT          885 a         2171 c         2260 g         1079 t
ORIGIN      
        1 gcgctgcatc gcctgcggct cggccgcgat gacgtgattg aggagcagca cgacacgctc
       61 catgaccgag cgttccgcca atgccttcat cgtctcgaac atcgacgaat tgtaggcagc
      121 cccttgcgac cacacccccc gatggcgaga ggcttctgac gcctctggac gacaatccgg
      181 cctttcccga cgctccatga tccttctcac cggcgccacc ggctacatcg catcgcacac
      241 gtggctggcc cttcattcgg caggcctgcg cgtcgtgggc ctcgacaact tcgccaacag
      301 ctcgccggag gtgctggagc gcctcgccgc gctcggcgtc gacgcccgcg ccttcgttcg
      361 cgccgatgtc accgatcgcg ccgccctcga cgaggtgttc aggcgctggc cgatcgaggc
      421 ggcgatccat ttcgcggcgc tcaagtcggt cggcgaaagc gtcgcccgcc cgctcgagta
      481 ccaccgcgtc aatgtcggcg gcctcgtcgc gctgctcggg gcgatgcagg ccgccgcctg
      541 ccgcacgttc gtcttcagct cgagcgccac cgtgtatggc gaacccgagc ggctgccgct
      601 tgccgaagac gcccgcctcg cggcgacgag cccgtacggc gcgaccaagc tgatcggcga
      661 agacgtgctg cgcgacctcg gtcggtccga ggctggctgg cgggtcggcg tgcttcgcta
      721 cttcaacccg gtcggcgcgc atccgagcgg aacgatcggc gaggatccgc gcgggacgcc
      781 gaacaacctc atgccctaca tcgcccaggt tgccgtgcgc cggcgccctc gcctgcaggt
      841 gttcggcgcc gactatccga cgcccgacgg caccggcgtg cgcgactatc ttcacgtcga
      901 cgacctcgcc gaggggcacg tcgcggcgct gcgccatctg agcagcggcg caccgtcttt
      961 caccgtcaac ctcggcacgg ggcggggagt gagcgtgctc gagctcgtgc gcgccttcga
     1021 gcgaagcagt ggccagccgg tgccgttcga gatcgtcggc cgccgccccg gcgacgtcgc
     1081 cgcctgctac gccgatccgg cgcgagcttg ctcgctgctc ggctggcggg cgactcgcga
     1141 tctcgatgcg atgtgcgccg acacctggcg ctggcagcag gccaatcccg acggcttcgc
     1201 ggcccgctga acgatgaaga tcaccacgac cgcgatcgat ggcgtcctcg tcatcgagcc
     1261 ccgggttttc ggtgacccgc ggggcttctt cctcgagtcg ttcaacgccc gtgatttcgc
     1321 tggcgcgacc ggcaggcacg tcgagttcgt ccaggacaac cagtcgcgct cgacgcgtgg
     1381 cgtcctgcgc ggcctgcact ggcaggaggc gccccatgcc cagggcaagc tggtccgggt
     1441 cgtcagtggg gcggtgttcg acgtcgccgt cgacatccga cgtgcttcgc cgaccttcgg
     1501 ccgctggatc ggcgtcgaac tgagcggcga caaccattgc cagatctgga tcgagccggg
     1561 cctggcacac ggctttctcg tgctgagcga gaccgccgac gtgctctaca agacgacgac
     1621 gtactacgcg cctgcggcgg agcggtcgat ccgcttcgac gaccccgatc tcgccatcgc
     1681 gtggcccgac gtcggcatgg cacccacggt ctcggcgaag gacaccgcgg gcatgtcgtt
     1741 cgctgcctgg cgcgccgagg cgagcgcgcg ctgatcctgg ctcgctgacg tatcgggacc
     1801 ggcgcagggc gggacgccga ttgcccccgc cgccggcgcc gggcgaacga ctcagcggcc
     1861 cgtctcgagc tggcgcggcg gggtgtagtt gttggcgcgg gccagcgaca ggtagcggga
     1921 ggcctcgacg tcgcgccggt cgacgttgcg aaagtaaagg ctcagccggt aggcagcgat
     1981 gccgttgccc aaccgggccg cgaagatcat ccaccgaccg tagtcggtac gctcgatgag
     2041 ctcgccgccg gcacccggct ggggcaaggt ttcggcgacg cgaagggccg cgtacttgtc
     2101 gccctgcaac gcccgccgca tgtcttcaac gacgcgcggt ggctgcccgg cggtcaattt
     2161 ggccggatcg agatcgagtc gagccgcgcg agcggcggcg accaccgtct cgagatcctt
     2221 caggcgcaag cgtgccacgt cggcgtcgtc gccctcgggc gtcttttgca gcaggtcgcg
     2281 cagttttgcc gccgcttcct cgggcgtggc cgcggcacgc gccgccgcca gctcgctggc
     2341 ggcggacgac gccggagccg gcggccgcgc gccgtccgtc ggcgccggct tcgacgccaa
     2401 ggccggatcc cacggcggat cgccgctcac gttcgacgcc agctcgggct gctgcgcgtt
     2461 cgggccgaac gccttgacga tggtcgggat ggaattgacc ttgttggcga ccttggcgcg
     2521 cacgctctcg agcgtgagga ggatcgagcg gtccggcggc gacgcggtga gctcggcgac
     2581 caggacttcg gcgaacagcg tgtcgcgctg cgggtcgttc ggcgcgaagg cccgcttgcc
     2641 gggcccggtc gcgaaggcca cgtggcaacc ctcgggaggc atcgtctggt tgtagccttc
     2701 gccgggcgat cgtggccggt cgggcgtgcg gcaggcgtcg atcatgatga cggtggccgc
     2761 cggtccatcc agtcgcctca ggtggctgaa gatgtccttc tcgggcgaga tgcaggtctt
     2821 cgaaagctgc gcgggaacgg cgtcgaggtc gctgccggcc ggcaccatga aattctcggc
     2881 gttcgactgg aagccgtggc cgacgaaata gaagacggcg ggcatccccg gattggcggc
     2941 gacggcctgc tgcatcttga cgatctcggc ctgcactgtc gacacgggag catcgtgcag
     3001 cgccaccacg tcgaagccga ggcgccgcaa ttgcggttcg atgtcgcgca gacatttctg
     3061 cgcgggcggc agattctcgg cggccgggtt gtacgaggta ttgccgacga gcaccgcata
     3121 gccggccttg gcctgtgctc gcgccggccc cggccagacg atcgcgccag cggccgcgac
     3181 ggccgcggcc tgcagcagcg gcctgcgccg caagcctttc acgacccgcc cgtcactggc
     3241 cacagcgcag cccggccgtc gcatcgcacg atgccggcgg cttcgccgaa tcgatgatgt
     3301 cgaccgccgg cggcgccagg cccgtctcgt agacgaggcc gcgctgcaat tgcttcctga
     3361 tgttttcctt gccgaaccca gacagcagcg cctccagcat cgcccggcgg aaaggttcct
     3421 gcgcatcggc gacgccttgc agatattcct tgaagcggat gtcgtcgaag cggctttgcg
     3481 ccaccgaaag gatgcgcacg gtgagcgggc cgccgcccgg ggcgatcggc gtcggcaaga
     3541 cgctgtagtc gatcgcgtcg gggcccgtca gcacgatgcc ggtcaccgtc accggcttgg
     3601 cctcgcccgg ggccggcgtg gcgacgctgc caaccgcgcc ggacgcatcg acgcccaccg
     3661 tatcgccggg cagcacgccg ttgactccgg caccggcggt gttgaggccg accgccgtac
     3721 tgccgtcgtc ggcccggttg acggcggtga tgccggtcgc ctgcacggcc agcggcgtga
     3781 tgttgacggt ggcgttgctc gcgtcggtga gcgtgtagtt gccggcgtcg ccgccgccga
     3841 gcgccaggcc gctgaccgtc acgggcttgg cggtgccgac gttcttgtcg gcgatggtgc
     3901 ccgaggcgcc agcgccgacg atcgacacgt tgtcgccggc gacggcattg ttgagcgtcg
     3961 cccccgccgt ggacacggcg acgacggttg tcgcgtcgta ggtgcggtcg accccggtga
     4021 tgccggtgga tgtcagaggc cgcggcgtga tggtcgcggt cgcgccgctg gcgtcggtga
     4081 cggtgtagtt gccggcgtcg gcgccgttga gcgcgacgcc cgtgatggtg accggcttcg
     4141 ccgcgccgac gttcttgtcg gccagcgtgc ccgtcgcgcc gccggcgatg ccgacggtat
     4201 cgccggcgat cacgccgccc aaggacgctc ccgagccgtt cacggcgacg gccgtcgtgc
     4261 cgtcatagac acgaccgacg ccggcgaagc cggtcgaggt gatggacctg gcgctgatcg
     4321 tggcggtcgc accgctcgca tcgctgaccg tgtagttgcc ctggtcgcca ccggtcagcg
     4381 ccaggccggt gatgctcacc gccttgttca cgccgatgtt cttgttcgcg agcgtaccga
     4441 cggcgctcgt cgtggcgagg ttgaccgtgt cgcccgcgac gaccccggtc agcgacgcac
     4501 cggccgcgtc gaggccgacg ccggttgtgc cgtcgtagac gcggtcgatg ccgtcgatgc
     4561 cgctcgcggt gatcggcttg ggcgtgatct gcgagaccgc gttgccgggc gtcccggcga
     4621 cgtgcgggcc ggcggcacgg gtgtatccgg cgaactgcag tccatagagc gtggcaccgt
     4681 tgtcgctcgg cgcgaccgcg ctggtgtcgt tgctcgggcc gaccgtgagc tgcttgttcg
     4741 ccccggcgtt cttgtcgacg aagttgccgg tgccgttgac ggtgtaattc gacagcgtca
     4801 gcgccaccgg ggtcgccgcg ttcagcgtgc cggcggacgt cgtgccggtc tgcgtgtagt
     4861 tgaagttggt cgtgccgtcg tagaccttcg agatcggcgg gttgtcggtg acgggcggcg
     4921 caaggctgcc ggtctggttc gtgaagagac cgttgcccgt accgggcgcc gcgcccgtgt
     4981 agccgccgta gacgatgtag ttgatgctgc tggaggcgac gccgaatccg agcgtaccga
     5041 aattgtcctg cgtgtagtcg ctcgaatgaa tcgtgaacct gccgcccacg ggcagcgtga
     5101 atggcgggtt gacgtaggag gtgttgttga cgaagccaac gccgctcatg tcgacgttcg
     5161 cggcagcaat cgtgtcgtcg agatagaaag cgccgccgat ggccgccagc gtgacattgc
     5221 ccgtcgcgct gccgctcgcg tcgaggtttc cgccggccgc gatgttcacg ttgcctgccg
     5281 cgttgaatgt cacgccgaag agagaattgc ccgggttgtt cagcgtgatg tcaccgcttc
     5341 cggtcgtggt gacatggaag aacccttcgc cggcgccgat gctcaggaca ccgagagcgc
     5401 cggcaggctg cgcgatgctg gtgttcaccc ccgtcacggc aaggtcggag gcggtgccgc
     5461 tcagcaccag cggcacgcca ggggcgttcg cgacgttgaa ctgggcatcg ccggcattga
     5521 cgaccaaccg gccgatctgg ttgttggcat ggctcaggtc gacgccgccg ggagggttga
     5581 atcccgaggc gccgtcgacg gtcagcgttc ccgcgctgac gatgccggcg ccgagcgaat
     5641 cctgctggat cgtcgtcgtc gcgctgtcgt tgttcgcgag cagcgacatc gtcgtcgcgg
     5701 tcgtcggcgc gttcaggttg atcgtcgtcc cggtcagcgt catcgtcgtg gcgttgatcg
     5761 cgccgttgac gttgacggtc ggcgccgtca ggttgacggt gcccgcgttg agcgaaccgc
     5821 cggagttgac gttgacctgg gccccgcccg acagcgagac agtcgccagt gaggtgactg
     5881 ccgcgttgac ctccacggaa ggcgaagtga tcggcgtcgt ctgcgcgctc agcgacgaat
     5941 tgttgagcgt catcgccgcg ttgagataca cgtttccgct ggtgagcgac agcgtcgtcg
     6001 ccgcgccgcc ggccgcgacc gtcagcggcg cagtcaccga caggctgatc gacgaggcga
     6061 gcgtgacgtc ggtggtgttg agcagcgacg cgacgcgggc cgccgtgatc tgactgttgg
     6121 cactcgggta atcgctcgca ttgagaacgt tgccggccag atcgtcgccg gaggttccgt
     6181 cgccattgac atcggcgacc gtgccgatga cgagagactg cggatcgagg agaagctggc
     6241 cggttcgccc cttgaccgcg cgcaggtcga catcgccctg gaaatcgagc agcttcttgc
     6301 ccgagacctc ggcttgcccg ccgtcaccgc cctgggcacc gccacgcgcg gcgatctgac
     6361 ccgcgtaact tgtctggtta tcggcccaga caacc
//
LOCUS       DASRWS010000488         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_488934, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000488 DASRWS010000000
VERSION     DASRWS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488934"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFF72_09195"
     CDS             <1..673
                     /locus_tag="VFF72_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZV93381.1"
                     /translation="TGAFKEPDKNLRAVFSVHPEPFTLVARKEAGITKFTDLKGKRVN
                     VGNPGSGTRSAMEELLAAEGMKMSDFALASELKADEHGPALCDNKIDAFFYGVGHPSA
                     NIQDPTTTCGAKLVPLDDPKIDALVAKHPYYAKVVIPGNLYANNPQPTPTYGVTATMV
                     TSAKEPDDVVYTITKAVFENLDEFKKLHPAFAHLDAQQMIKNGISAPIHPGALRYYRE
                     KGWMK"
     gene            681..>2113
                     /locus_tag="VFF72_09200"
     CDS             681..>2113
                     /locus_tag="VFF72_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HZV93382.1"
                     /translation="MSGESVDAAQKLVQEADLGGRMPAGWPARLIAGVCLAWSLLQLW
                     YASPLPFTFDVFVLNSTEMRSLHLGLGLFLAYLVFPFAKRSPRDRIPLHDWVMAGVAA
                     FCGAYLFLFYREISARPGEPTTMDIAAAIAGMILLLEATRRVVGPPLAIIALLMLVYA
                     FAGPHMPDVIAHKGVSLSKAVSHFWLSTEGVFGVALGVSASYIFLFVLFGSLLEKAGA
                     GNYFIKVAFALLGHMRGGPAKAAVVSSGLMGMISGSSVANVVTCGTFTIPLMKRVGYS
                     PEKAGAIEVAAGVDGQIMPPVMGAAAFLMAEYVGVPYSEIVKNAFLPAIISYIALFYI
                     VHLEACKSGIAGLPSRPGTKPWLRVAGIFMTLAGIVIVGNIVYWGLGWLRPLLGGGAS
                     LLVLALIAVAYVLLVRQVASEPDLTIDDPNAPVIELPPLRDTILSGLHFLLPIVVLVW
                     ALLVEQLSPGLSAFYAAVLLIIILVTQ"
BASE COUNT          318 a          768 c          645 g          382 t
ORIGIN      
        1 caccggcgcg ttcaaggaac ccgacaagaa cctgcgtgcc gtcttctcgg tgcaccccga
       61 gccgttcacg ctggtcgcgc gcaaggaagc cgggatcacc aagttcaccg acctcaaggg
      121 caagcgcgtc aacgtcggca accccgggtc gggcacccgc tcggccatgg aagagctgct
      181 cgccgccgag ggcatgaaga tgagcgactt cgccctcgcc tcggagctga aggccgacga
      241 gcacggcccg gcgctgtgcg acaacaagat cgacgctttc ttctacggcg tcggccaccc
      301 gtcggccaac atccaggatc cgaccacgac ctgcggcgcc aagctcgtgc cgctcgacga
      361 tccgaagatc gacgcgctcg tcgccaagca tccgtactac gccaaggtcg tgatccccgg
      421 caacctctac gccaacaatc cgcagccgac gcccacctac ggcgtcacgg cgacgatggt
      481 gacctcggcc aaggaacccg acgacgtcgt ctacacgatc accaaggcgg ttttcgagaa
      541 cctcgacgag ttcaagaagc ttcacccggc gttcgcccac ctcgacgcgc agcagatgat
      601 caagaacggc atctccgcgc cgatccatcc gggggcgctg cgctactacc gcgagaaggg
      661 ctggatgaag tgaggcggcg gtgagcggcg aaagcgtcga cgcggcgcag aagctggtcc
      721 aggaggccga cctcggcggg cgcatgcccg ccggctggcc cgcgcgcctg attgccgggg
      781 tctgcctggc ctggtcgctg ctgcagctct ggtacgcctc gccgctgccg ttcaccttcg
      841 acgtcttcgt tctcaattcg accgagatgc gctcgctgca tctcggcctg ggcctctttc
      901 tcgcctatct cgtcttcccc ttcgccaagc gctcgccgcg cgatcgcatt cccctgcacg
      961 actgggtgat ggcgggagtg gcggccttct gtggcgccta cctctttctc ttctaccgcg
     1021 agatctcggc gcggccgggc gagccgacga cgatggacat cgccgccgcc atcgccggga
     1081 tgatcctgct gctcgaggca acgcgacgcg tcgtcgggcc gcccctggcg atcatcgccc
     1141 tgctcatgct cgtgtatgcc ttcgccggcc cgcacatgcc cgacgtcatc gcccacaaag
     1201 gcgtctcgct gagcaaggcc gtctcgcatt tctggctttc caccgagggc gtcttcggcg
     1261 tcgcgctcgg agtctcggcg agctacatct tcctgttcgt tctgttcggt tcgctgctcg
     1321 agaaagccgg cgccggcaac tacttcatca aggtcgcgtt cgccctgctc gggcacatgc
     1381 gcggcggccc ggccaaggcc gccgtcgtct cgtccggcct gatggggatg atctccggct
     1441 cgtcggtggc caacgtcgtc acgtgcggca ccttcacgat cccgctgatg aagcgggtcg
     1501 gctactcgcc cgagaaagcc ggcgcgatcg aggtcgcggc cggcgtcgac ggccagatca
     1561 tgccgccggt gatgggcgcc gccgcgttcc tgatggccga gtacgtcggc gtgccgtact
     1621 ccgagatcgt caagaacgcc ttcctgccgg caatcatttc ctacatcgcc cttttctaca
     1681 tcgtccacct cgaggcctgc aaatcaggca tcgcgggcct gccgagccgg cccggcacga
     1741 agccgtggct gcgcgttgcc ggcatcttca tgaccctcgc cggcatcgtc atcgtcggca
     1801 acatcgtcta ctggggcctg ggctggctgc gcccgctgct cggcggaggc gcatcgctgc
     1861 tcgtgctcgc gctgatcgcg gtggcctacg tgctgctggt gcgccaggtc gcctccgagc
     1921 ccgacctgac gatcgacgac ccgaacgctc cggtcatcga gctgccgccg ctgcgcgaca
     1981 cgatcctttc cggccttcat ttcctgctgc cgatcgtcgt cctcgtctgg gcgctcctcg
     2041 tcgagcagct ctcgcccggg ctctcggcgt tctacgcggc ggtgctgctc atcatcatcc
     2101 tcgtcacgca aaa
//
LOCUS       DASRWS010000489         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3076670, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000489 DASRWS010000000
VERSION     DASRWS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076670"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFF72_09205"
     CDS             <1..548
                     /locus_tag="VFF72_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZV93383.1"
                     /translation="LQPHFLFNALNTVSSLIHSDPDLADSLLTRLATLLRAATDAGRR
                     VEQPLRNELELLRAYADIMGERFADRVAIDWEIDVDAGRCLVPTLGLQPLLENCFRHV
                     VERRRTPTRVVIRARCDAGRLRVEVQDDGDLRALPLSRGVGLANLERRLHALHGDRAS
                     LALRLNAGGGLIACLDLPCAC"
     gene            542..1255
                     /locus_tag="VFF72_09210"
     CDS             542..1255
                     /locus_tag="VFF72_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV93384.1"
                     /translation="MLIVDDEAPARRKLRRMLGAFADIEVAGEAADGAAALALVGELA
                     PDVLFLDVQMPEVDGFDVAASLPDDGPAIVFVTAFDRYALEAFDTRAADYLLKPVEPA
                     RLERAVQRLRSASPAPAERPAAAASPTQLLIAERGVTHVVPVADIEWLQAADNYVDVH
                     LRGRTLLMRRTLESLLKDLAPAFVRTHRSAAVALSRVQALRPRGNGDATVLLQSGAEV
                     PCSRAHRAVLVRHLRDRSR"
     gene            1419..2351
                     /locus_tag="VFF72_09215"
     CDS             1419..2351
                     /locus_tag="VFF72_09215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZV93385.1"
                     /translation="MEVLYTILKAAVVTVLVSSLLEALVLSCRRGWRSYDWKAAGVSV
                     VDFLVREYPLRFLLPLAFWTDAMGWFWQHRLFTLPMHHWTGWAACFVGAEFCYYVYHR
                     TAHRVRWFWCTHAVHHSPNQLNLSAAYRFGWTGRLTGTLAFFMLAPLLGMPPRIVLML
                     LSLNLLYQFWIHATWIPRLGPLEWILNTPSAHRVHHAANLEYLDGNYGGVLIVFDRLF
                     GTYIAERDDVPCRYGLVEPITSYNLFAVEFHQWRALLRDLRGARSLREAWGYVAKPPG
                     WRPDGRGRTTEDLRGDLVATTKAATAGAASPASA"
     gene            complement(2359..3414)
                     /locus_tag="VFF72_09220"
     CDS             complement(2359..3414)
                     /locus_tag="VFF72_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZV93386.1"
                     /translation="MNPVLVEALRGGVRESAHRGAVAVVDADGHVVATIGDIERPIFP
                     RSAVKVLQALPLVESGAADRLNLGDEELAVACASHNGEPEHVAVVARMLAKAGLDAGA
                     LECGAHWPYREKVQRDMARAGIEPSALHNNCSGKHAGFLCVACQLASQPGAGVDRSGL
                     RRFAAGYVGPAHPVMREVTASLQAATGFDLDQAARGTDGCSIPTFAIPLRHLALAFAR
                     VGTGAHWPAGRAAAAQRLRVAVARHPFMVGGSDRFDTRVMQRLGERVFCKVGAEGVFC
                     AALPGRGLGVAIKMDDGNNARAAEVAMAAAIEACVDLDDGEAQFMRGLSDVTLRNWNG
                     IEVGALRAAAELREKLG"
     gene            3528..>3772
                     /locus_tag="VFF72_09225"
     CDS             3528..>3772
                     /locus_tag="VFF72_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZV93387.1"
                     /translation="MPATALALVLAAALLHALWNLVAKKSGGDSRFALIAAVFLAVLW
                     APVGVWAAWNVVPRWRLIEWSVLIASAIVHVVYFTTLL"
BASE COUNT          482 a         1382 c         1302 g          606 t
ORIGIN      
        1 agctgcagcc gcacttcctg ttcaacgcct tgaacaccgt gtcgtcgctg atccattccg
       61 atcccgacct cgccgactcg ctcctcaccc ggctggcgac cttgctgcgc gccgccaccg
      121 acgccggccg ccgtgtcgag cagccgctcc gcaatgagct cgagctgctg cgcgcctacg
      181 ccgacatcat gggcgagcga ttcgccgatc gcgtcgcgat cgactgggaa atcgatgtcg
      241 acgccggccg gtgtctcgtg ccgacgctcg ggctgcaacc gctgctcgag aactgctttc
      301 gtcacgtcgt cgagcgacgt cgcacgccca cccgcgtggt gattcgtgcg cgctgcgacg
      361 ccggccggct gcgcgtcgaa gtgcaggacg acggcgacct gcgcgcgctg ccgctgtcgc
      421 gcggcgtggg cctggccaac ctcgagcggc gacttcacgc gctgcacggt gatcgcgcga
      481 gcctcgcgct gcgcctgaac gccggcggcg gattgatcgc ctgcctggac ctgccttgcg
      541 cgtgctgatc gtcgacgacg aggcaccggc gcggcgcaag ctgcgccgga tgctcggcgc
      601 tttcgccgac atcgaggtcg caggcgaggc ggccgacggc gccgccgccc tggcgctcgt
      661 cggcgagttg gctcccgacg tgctcttcct cgatgtgcag atgcccgagg tcgacgggtt
      721 cgacgtcgcg gcatccttgc ccgacgacgg gccggccatt gtcttcgtga ccgccttcga
      781 ccgctatgcg ctcgaggcct tcgacacccg cgccgccgac tacctgctca agcccgtcga
      841 gccggcgcgc ctggagcggg ccgtccagcg cctgcgctcg gcatcgcccg cgccggccga
      901 aaggccggcc gccgcggcgt cgccgaccca gctgctgatc gccgagcgcg gcgtcaccca
      961 tgtcgtgccc gtggccgaca tcgagtggct ccaggccgcc gacaactacg tcgacgtcca
     1021 cctgcgcggc aggacgctgc tgatgcggcg cacgctcgag tccttgctga aggacctggc
     1081 accggcgttc gttcgcacgc atcgcagcgc ggccgtcgcg ttgtcccgtg tccaggcgct
     1141 gcggccacgc ggcaacggcg acgccaccgt gctgctgcag agcggcgccg aagtgccttg
     1201 cagccgcgct catcgcgccg tcctggtccg gcacctgcgg gaccgttcgc gctgatcccg
     1261 tcgcgtcgcc cggcgcgccg tcgctgcgag gaagcggacc gcgactcgcc ccgcgccgtc
     1321 gacgtttcgt cccatcgcgc ttgccacgtt cgggcggcac ccttagcgtg cgggccgtgg
     1381 ctgaacgatg cacgccggca cgccggcgcg aatgacccat ggaagtgctc tacacgatcc
     1441 tcaaggccgc cgtcgtcacg gtgctcgtgt cgtcgctgct cgaggcgctg gtgctgtcgt
     1501 gccggagggg ctggcgcagc tacgactgga aggccgcggg cgtttcggtc gtcgatttcc
     1561 tggttcgcga gtacccgttg cgcttcctgc tgccgctggc cttctggacc gatgcgatgg
     1621 gctggttctg gcagcaccgg ctgttcacct tgccgatgca ccactggacg ggctgggcgg
     1681 cctgcttcgt gggtgccgag ttctgctact acgtgtacca ccgcaccgcg caccgggtgc
     1741 gctggttctg gtgcacgcat gccgtgcacc actctcccaa ccagctcaac ctttcagcgg
     1801 cctaccgttt cggctggacc ggcaggctca ccggaacgct ggcgttcttc atgctcgcgc
     1861 cgctcctggg catgccgccg cgcatcgtgc tgatgctgct gtcgctcaac ctgctctacc
     1921 agttctggat ccacgcgacc tggattccgc gcctcggccc gctggagtgg attctcaaca
     1981 ccccttcggc gcaccgtgtc caccacgccg ccaacctcga gtacctcgat ggcaactacg
     2041 gcggcgtgct gatcgtcttc gaccggctct tcggcaccta catcgccgag cgcgacgacg
     2101 tgccgtgtcg ctacggcctg gtcgagccga tcacgagcta caacctgttc gccgtcgagt
     2161 tccatcaatg gcgggcgctc ttgcgcgacc tgcgcggtgc gcgttcgctg cgcgaggcct
     2221 ggggctacgt ggcgaagccg cccggctggc ggcccgacgg ccgcggcagg acgaccgagg
     2281 acctgagggg cgacctcgtc gcgacgacga aggccgcgac cgccggtgcc gcgtcgcccg
     2341 catcggcgta ggcgctcgtc agcccagctt ttcgcgcagc tcggctgccg cgcgcagcgc
     2401 gccgacctcg atgccgttcc agttgcgcag cgtcacgtcg ctgaggccgc gcatgaactg
     2461 cgcctcgccg tcgtcgagat cgacgcaggc ctcgatcgcg gccgccatcg ccacttcggc
     2521 ggcgcgggcg ttgttgccgt cgtccatctt gatggcgacg ccgaggccgc ggccgggcag
     2581 ggcggcgcag aagacgccct cggcaccgac cttgcagaac acccgctcgc cgagccgctg
     2641 catcacccgg gtgtcgaacc ggtcgctgcc gccgaccatg aagggatgtc gagcgacggc
     2701 gacgcgcagc cgttgcgccg cggcggcgcg cccggccggc cagtgagcgc cggtgccgac
     2761 gcgcgcgaag gcgagcgcga ggtggcgaag cgggatcgcg aaggtcggga tcgagcagcc
     2821 gtcggtgccg cgcgcggcct gatcgaggtc gaagccggtc gccgcctgca atgacgcggt
     2881 gacctcgcgc atcaccggat gcgccggacc gacgtagccg gcggcgaagc ggcgaagccc
     2941 gctccgatcg acgccggcac cgggctggct cgcgagctgg caggccacgc acaggaaccc
     3001 ggcgtgcttg cccgagcagt tgttgtgcaa cgcgctcggc tcgatgccgg cccgtgccat
     3061 gtcgcgctgc accttctcgc ggtagggcca gtgcgcgccg cattcgaggg caccggcgtc
     3121 gagacccgcc ttggcgagca tgcgggcgac gacggcgacg tgctcgggct cgccgttgtg
     3181 cgaggcgcag gcgacggcga gctcttcgtc gccgagattc agccgatcgg cggcgccgct
     3241 ttcaacgagc gggagggcct gcagcacctt caccgccgag cgcggaaaga tcgggcgctc
     3301 gatgtcgccg atcgtcgcga cgacgtggcc atcggcatcg acgacggcca ccgcgccgcg
     3361 gtgcgcggat tcgcgcacgc cgccgcgcaa cgcctcgacg aggacgggat tcatgcagcc
     3421 gcggccggtg ccatggcggg aactgtagcg cgacgggcct tcgccggggc cggacgggac
     3481 catggcgaca gggccggcga gcgtgtcctc cacaatggac ttcttccatg ccggccaccg
     3541 ccctcgcgct cgtcctcgcc gcagccttgc tgcacgcgtt gtggaacctg gtcgcgaaga
     3601 agtcgggcgg cgactcgcgg ttcgccctca tcgcggcggt ctttctcgcg gtgctctggg
     3661 cgccggtcgg cgtgtgggcg gcttggaacg tcgtcccccg ctggcgcctc atcgaatggt
     3721 cggtgctgat cgccagcgcg atcgtccacg tcgtctactt cacgacgttg ct
//
LOCUS       DASRWS010000490         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3933272, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000490 DASRWS010000000
VERSION     DASRWS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933272"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFF72_09230"
     CDS             <1..823
                     /locus_tag="VFF72_09230"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HZV93388.1"
                     /translation="SLVHTDLAGNDLPDPPNVRNYLISGTQHAGPAGANSLGVCQQFG
                     NPIDQNPALRALWVDLDQWLDGTPPPPSAVPQRATGTAVFSATTADSPLGIGTVSQSD
                     LGWPTIPGVTYTGLITVHNLWNWGSQFDSGILSNSPPVATGKVYPSFVSKVDSDGNEI
                     AGIRLPGVAAPVATSAGWNLRSAAYGGNDGCESTGSYIPFAPTAIARTALGDPRPSLD
                     ERYGSHAGYVAAVTSAVNALEAQRLLLPADGQAYITAAQQPVNVVGNPLYGSYSW"
     gene            complement(938..1855)
                     /locus_tag="VFF72_09235"
     CDS             complement(938..1855)
                     /locus_tag="VFF72_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZV93389.1"
                     /translation="MTGTRTQTFAMLAALSLCPVLAQAHGVESHGAAAATLGALPWTF
                     EPWEIGCLLVSAALYAAGVWRLWRHAGVGRGVGFTEVAEFALGLLAIVAALVSPLDAL
                     SDSLFSAHMVEHELLMIVAAPLLVLARPLAAWAWALPASWRGAIGGFFHRPGWRRPWL
                     LFTGPFWAWLLHLLALWLWHIPALFEAATANEGIHAFQHICFLGTALVFWWSVLGKAT
                     RHHRGIALLSIFATMIHTGALGALLTLSNVVWYSSYLATTSAWGLTPLQDQQLGGIIM
                     WVPTGLVYIACGLWLMTRWLSEAPRFSRA"
     gene            complement(1852..>2039)
                     /locus_tag="VFF72_09240"
     CDS             complement(1852..>2039)
                     /locus_tag="VFF72_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93390.1"
                     /translation="SPGCSVGTPEESVGVMVILFLSSRSSVPAGCDASATGVPNPGKE
                     KPSATFFHSNSTHENRG"
BASE COUNT          357 a          724 c          693 g          265 t
ORIGIN      
        1 ctcgctggtc cacaccgacc tcgccggcaa cgacctcccc gatccgccga acgtgcgcaa
       61 ctacctgatc tccggaacgc agcacgccgg cccggcgggc gcgaattcgc tcggcgtctg
      121 ccagcagttc ggcaacccga tcgaccagaa cccggccctg cgtgcgctct gggtcgatct
      181 cgaccagtgg ctcgacggca cgccgccgcc gccgagcgcg gtgccgcagc gcgcgacggg
      241 cacggcggtc ttctcggcga ccaccgccga ttcgccgctc ggcatcggca cggtgtcgca
      301 aagcgacctc ggctggccga cgattccggg cgtgacctac accggcctga tcacggtgca
      361 caacctctgg aactggggct ctcagttcga cagcggcatc ctgtcgaaca gtcctccggt
      421 ggcgaccggc aaggtctatc cgtcgttcgt ctcgaaggtc gattccgacg gcaacgagat
      481 cgccggcatc cggctgccgg gcgtcgctgc gccggtggcg acgtcggcgg ggtggaatct
      541 gcgcagcgcc gcctacgggg gcaacgacgg ttgcgagtcg acgggcagct acatcccgtt
      601 cgcgccgacg gcgatcgccc gcaccgcgct cggcgatccg cggccgtcgc tcgacgaacg
      661 ctacggcagc cacgccggct acgtcgcggc cgtcacgtcg gcagtgaacg cgctcgaggc
      721 gcaacgcctg ctcctgccag ccgacggaca ggcttacatc acggcggccc agcaaccggt
      781 gaacgtcgtc ggcaatccgc tctacggcag ctacagctgg tgagcggtgc gatctcgccg
      841 cgcgagcggc gggatcgcag gcgccctgtc gccggggccg gcttccgggt cacgcgccat
      901 ccgtgcgcga tccggggctc ggccccggtc gcgttcgtca ggcccggctg aaccgcggcg
      961 cctccgacaa ccaacgagtc atcagccaca ggccgcaggc gatgtagacg aggccggtcg
     1021 gcacccacat gatgatgccg cccagttgct gatcctgcag gggcgtcagg ccccaggccg
     1081 aggtggtggc caggtagctc gagtaccaga cgacgttcga gagcgtgagc agcgcgccca
     1141 gcgcgcccgt gtggatcatg gtggcgaaga tggagagcaa tgcgatgccg cggtggtgcc
     1201 gcgtcgcctt gccgagcacg ctccaccaga agacgagcgc ggtgccgaga aagcagatgt
     1261 gctggaaagc gtggatgccc tcgttggcgg tcgccgcttc gaacagcgcc ggaatgtgcc
     1321 agagccacag ggcgagcaag tggagaagcc aggcccagaa cggccccgtg aaaagcagcc
     1381 acggacgccg ccagccgggt cgatggaaga agccgccgat cgcgcctcgc cagctggccg
     1441 gcaacgccca cgcccacgcc gcgagcggac gggcgagaac gagtagcggc gccgcgacga
     1501 tcatcaacag ctcgtgctcg accatgtgcg ccgaaaacag cgaatcggac aacgcgtcga
     1561 gcgggctgac gagggcggcg acgatcgcca gcaggccgag tgcgaattcg gcaacctcgg
     1621 tgaacccgac gccgcggccg accccggcgt gacgccacag ccgccacacc cctgccgcat
     1681 acagcgccgc gctcacgagc aggcagccga tctcccaggg ctcgaaggtc cacggcaacg
     1741 cgcccagggt ggctgcggcg gcgccgtgcg actcgacgcc atgcgcctgc gcgaggacgg
     1801 ggcaaagtga cagcgccgcg agcatcgcga aggtctgcgt acgtgttccg gtcatccccg
     1861 attctcgtgc gtcgaattcg aatggaagaa cgtcgccgag ggtttctcct tgcccggatt
     1921 gggcacgcca gttgccgacg catcgcatcc cgccggcact gaagaccgac tcgacaagaa
     1981 caggatgacc atgaccccga ccgattcctc cggcgtgccg acagagcagc ccggggatt
//
LOCUS       DASRWS010000491         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3933503, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000491 DASRWS010000000
VERSION     DASRWS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933503"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="rpoE"
                     /locus_tag="VFF72_09245"
     CDS             <1..600
                     /gene="rpoE"
                     /locus_tag="VFF72_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HZV93391.1"
                     /translation="PDADAPLIERVKQGDVKAFEMLVVKYQRRIERLIGRMVRDVDLV
                     PDIAQETFIRAYRAIPKFRGESAFYTWLYRIAVNTAKKSLMELKRDPLVTESARASRD
                     EDDDSPRFDHELTDGETPEAVLASKQIAAAVNFAIDGLSEELRQAITLREIEGLSYEE
                     ISELMNCPIGTVRSRIFRAREAIAAKLRPLLDTRQGERW"
     gene            668..1321
                     /locus_tag="VFF72_09250"
     CDS             668..1321
                     /locus_tag="VFF72_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HZV93392.1"
                     /translation="MASNPVEGSTDAERLSALVDGELDEAALAETCFEWAHEAQARMR
                     WHAYQVIGDVLRSDDLATGVCHDEQFLVSLRARLADEPVIVAPAPLSTPPLPLRASMS
                     VTRPLPRWTIPSAIAAGVVLVVGTFTVLRPLEPAPSGAAPVAVAEATPPAAVASPLQA
                     ASIREPVEPEAIIVDGKMIHDARLDRYLAAHKQFSGSSALGLPSAFLRAATVEADAR"
     gene            1422..2375
                     /locus_tag="VFF72_09255"
     CDS             1422..2375
                     /locus_tag="VFF72_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HZV93393.1"
                     /translation="MAATPQAPAASEVRSWLLRIHEAASHRNFQGTFVVSGGGNVASA
                     HIAHFVEGPNQYERIESLDGRQRKVFRINNVVHTVWPASHVAMIEQRGLLNSFPALLQ
                     AGDDGLGEWYEVRPEGTDRVAGHGADILEVRARDAYRYGYRLWADHASGLLLRVDVIG
                     VHGHVLETSAFSDVSIGVRPQPASIVQAMRRLDGYRVVRPVLTPTRLDKEGWALRRVA
                     PGFKEVSCVSRQIESPREVRADAQALDPVIQTIFSDGLTYVSVFIEPYRPDRHGHPML
                     AALGATLTLSERHGDWWVTIVGDTPQATLKLFAAALERKKP"
     gene            2530..>3207
                     /locus_tag="VFF72_09260"
     CDS             2530..>3207
                     /locus_tag="VFF72_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZV93394.1"
                     /translation="MLAAAAPHVAQAQALPDFTELVEKVGPAVVNIRTTERLRASRNG
                     SGDIDEDMLEFFRRFGIPLPNTRPNPNPRSPEMPGDGESRPRGIGSGFILNADGYVMT
                     NAHVVDGADEVVVTLADKREFKAKIIGADKRSDVALVKIDASGLPTVRIGDVSKLKVG
                     EWVIAIGSPFGLESTVTAGIVSAKSRDTGDLLPLIQTDVPINPGNSGGPLINMRGEVV
                     GINSQIYS"
BASE COUNT          520 a         1106 c         1052 g          529 t
ORIGIN      
        1 cccgacgccg acgccccgct gatcgaacgg gtcaagcagg gcgacgtcaa ggctttcgag
       61 atgctggtcg tcaagtacca gcgccggatc gaaaggctca tcggacgcat ggtgcgcgat
      121 gtcgacctgg ttcccgacat cgcccaggag acattcatcc gcgcctatcg ggcaatcccg
      181 aagtttcgcg gcgaaagcgc gttctacacc tggctctacc ggatcgcggt caacacggcg
      241 aaaaagtcgc tgatggaact caagcgcgac ccgctcgtca ccgagtcggc ccgtgccagc
      301 cgcgacgagg acgacgattc gccgcgcttc gaccacgagc tgaccgacgg cgaaaccccc
      361 gaggcggtcc tggccagcaa acagatcgcg gcggcagtga actttgcgat cgatggcttg
      421 tccgaagaat tgcggcaggc gatcacgctg cgcgagatcg aaggtctgag ctacgaggag
      481 atctccgagc tgatgaactg cccgatcgga acggtgcgct cgcggatatt cagggcgcgg
      541 gaagcgatcg ccgccaagct gcggccgctt ctcgacaccc gccagggcga gcgatggtga
      601 ctggcgtcag gatgaagcgg cgggcaccgg gcccgtcctt cgcattgcaa tagcggtgga
      661 gagcggcatg gcttcgaatc cggtggaagg gtcgaccgac gcggagcgcc tgtccgcgct
      721 cgtcgacggc gaactcgacg aggcggcgct ggcggagacc tgcttcgagt gggcgcatga
      781 agcgcaggcg agaatgcgct ggcatgccta tcaggtcatc ggcgacgtgc tgcgctcgga
      841 cgatcttgcg acaggcgttt gccacgacga gcagttcctg gtgtcgctgc gagcccggct
      901 cgccgatgag ccggtgatcg tcgcacctgc accactttcg acgccgcccc tgccgctgcg
      961 cgccagcatg tcggtcacca ggccgcttcc gcgctggacg attccgtcgg caatcgccgc
     1021 gggcgtcgtt ctcgtcgtcg ggacgttcac ggtgctccgg ccgctcgagc cggcgccgag
     1081 cggagcggcc ccggtcgccg tcgccgaggc aacgccgccc gccgcggtcg cgtcgccgct
     1141 gcaggcagcc tcgatccgcg agccggtcga gcccgaggcg atcatcgtcg atggcaagat
     1201 gatccatgac gcgcgtctcg accgctatct ggcggcgcac aagcagtttt ccggctcttc
     1261 cgcgctcggg ctgccgtcgg ctttcctgcg cgccgcgacg gtcgaagccg acgcgcgctg
     1321 atcgcgacgc gttttcttcc tcgatgtcgt ctcgcgcttt cgcttcattc gccttcgcga
     1381 cggcggctct tgtttgcggc ttcgccccgt cggcggccgc tatggcggcg acgccgcagg
     1441 cgcccgcagc cagcgaagtg cgctcgtggc tgttgcgaat tcacgaggcg gcgagccacc
     1501 ggaacttcca gggaacgttc gtcgtgagcg ggggcggcaa tgtcgccagc gcccacatcg
     1561 ctcatttcgt cgaaggcccc aaccagtacg agcgcatcga gtcgctcgat ggccgccagc
     1621 gcaaggtctt tcgcatcaac aacgtggtgc acacggtctg gcccgccagc cacgtcgcca
     1681 tgatcgagca gcgcggcctg ctgaattcgt ttccggcgtt gctgcaggcg ggcgacgacg
     1741 gcctcggcga gtggtacgag gtgcggcccg agggcaccga ccgcgttgcc ggtcacgggg
     1801 ccgacatcct cgaggtgcgc gcgcgggacg cctatcgcta cgggtatcga ctctgggccg
     1861 accacgcctc gggtctgctc ctgcgtgtcg atgtcatcgg cgtgcacggt cacgtgctcg
     1921 agacctcggc cttctccgac gtctcgatcg gcgttcgtcc ccagccggcg agcatcgtcc
     1981 aggcgatgcg ccggctcgac ggctatcgcg tcgtgaggcc ggtcctgacg ccaacgcgtc
     2041 tcgacaagga aggctgggcg ctgcgccgcg tcgcgcccgg cttcaaggag gtgagctgcg
     2101 tcagtcggca gatcgaaagc ccgcgcgaag tccgcgccga cgcgcaggcg ctggatccgg
     2161 tgatccagac gatcttttcc gacggactga cctatgtttc cgtcttcatc gagccctatc
     2221 gtccggaccg gcacggccat ccgatgctcg cggcgctcgg tgcgacgctg acgctcagcg
     2281 agcgacatgg tgactggtgg gtgaccatcg tcggcgacac tccgcaggcc acgctcaagc
     2341 ttttcgcggc tgcgctcgag cgcaagaaac cgtagccggc ggcggccggc cggacacggc
     2401 acacttccgg tcctgttccg aagttcgctt gtccgactcg acgaaagctt tcgcatgcca
     2461 ccgtccacca cgtctactgc cctcgcggcc ggccgcgccg cgttcgccgc tatcgccctg
     2521 tcgatcggca tgctggccgc tgccgcgccc catgtcgcgc aggcccaggc gctccccgat
     2581 ttcaccgagc tggtggagaa ggtcggaccg gccgtcgtca acatccgcac gaccgagcgc
     2641 cttcgcgcct cccgcaacgg ctcgggcgac atcgacgaag acatgctcga gttcttccgg
     2701 cgcttcggaa ttccgttgcc gaacacccgg ccgaacccga atccacgatc gcccgaaatg
     2761 ccgggcgacg gcgaatctcg gccccgcggc atcggctcgg gattcatcct caatgccgac
     2821 ggctacgtca tgaccaatgc gcacgtggtc gacggcgccg acgaggttgt cgtgacgctc
     2881 gccgacaagc gcgagttcaa ggcaaagatc atcggcgccg acaaacgatc cgacgtcgcg
     2941 ctcgtgaaga tcgatgccag cggcctgccg acggtgcgca tcggcgacgt gagcaagctc
     3001 aaggtcggtg aatgggtgat cgcgatcggc tcgccgttcg gcctggaaag caccgtcacc
     3061 gccggcatcg tcagcgcgaa atcgcgcgac acgggcgatc tgttgcccct gatccagacc
     3121 gacgtgccca tcaaccccgg caactccggc ggcccgctca tcaacatgcg tggcgaggtc
     3181 gtcggcatca actcccagat ctacagc
//
LOCUS       DASRWS010000492         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2779807, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000492 DASRWS010000000
VERSION     DASRWS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2779807"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFF72_09265"
     CDS             <1..322
                     /locus_tag="VFF72_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZV93395.1"
                     /translation="FLAEHPDFAVHLSHKYVRMVRTLTEQVKRLALQDVYGRMANVLV
                     DLSDVVGTERVVREKLTQQDIADRIGCSREMVNRVLKDLTTGGYVSVRDDRYVIHRKL
                     PAGR"
     gene            complement(421..744)
                     /locus_tag="VFF72_09270"
     CDS             complement(421..744)
                     /locus_tag="VFF72_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93396.1"
                     /translation="MLNTLDAPARTNEIDARWYAGTVFPLWNASVAPTRAIEYRRAER
                     WRPCKVGNAWRVGRRLPGQARRVDFHGGNPQPLEMNEAEATAFAAALNRRLETWLYAD
                     PARVY"
     gene            complement(832..2013)
                     /locus_tag="VFF72_09275"
     CDS             complement(832..2013)
                     /locus_tag="VFF72_09275"
                     /inference="COORDINATES: protein motif:HMM:NF036878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZV93397.1"
                     /translation="MDIPAVQATLRRFAAELLSFADRTSIELDASPGSKTHVLIDWEN
                     VQPSDVDVRELVPDATDLWLFHGPNQKNVAAHHATFGDRATPVRIARTGKNALDFHLT
                     FYIGYIASRQPEARFIVISNDKGYGPMLEHAKELGFAADQVGFVRLKGRGAATKSAAA
                     KARGAGTATKAARKTRPSAAAPTRVNAATGPLEAATGGAAKAATATRRSAPTKTPAKR
                     SAQESTRADAAPQTDARAAKAASSRTRPRPSTPPAPAAGKSAPKAAKATPAPAPAAKG
                     IRRPAAGKTAKAEATSSAAAAPSRRRKPATAAAESPSRVDPGRAASPRTGKSFEQVVA
                     LLGKRPVDRLPHKQARLLADVAQMIGAAADSGEARAMLDRLVASGKVSIDERRTVRYV
                     L"
     gene            2136..2597
                     /locus_tag="VFF72_09280"
     CDS             2136..2597
                     /locus_tag="VFF72_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZV93398.1"
                     /translation="MSLASVRAFLADRAPDIEILEASSSTATVALAAEAHGVTPGQIA
                     KTLSLRLGGEAILVVMRGDARLDNRKFKDRFGAKPKMLDAAEVEQATGHPVGGVCPFG
                     LPAKLRVYADVSLRAFDVVVPAAGDTHTALRVAPVRLAELAGAEWIDVAQP"
BASE COUNT          350 a          907 c          962 g          444 t
ORIGIN      
        1 gttcctcgcc gagcatcccg acttcgcggt gcacctctcg cacaagtacg tgcgcatggt
       61 gcgcaccctg accgagcagg tgaagcggct ggcgctgcag gacgtgtacg gccggatggc
      121 gaacgtgctg gtggatcttt ccgacgtcgt cggcaccgag cgggtcgttc gcgagaagct
      181 gacgcagcag gacatcgccg atcgcatcgg ctgctcgagg gagatggtga accgggtgct
      241 gaaggacctg acgaccggcg gatacgtgtc ggtgcgcgac gatcgctacg tcatccaccg
      301 caagctgccg gccggccgat agccggcccg gtcaccggca acgggccggc tacccccgcc
      361 cgttcaccgc agcgccgcgg cggcgacgcc acacgaggac ttccccgggg ggttagcgcg
      421 tcaatagacg cgcgccggat cggcgtagag ccaggtctcg aggcggcggt tgagggccgc
      481 ggcaaaggca gtcgcctcgg cttcgttcat ttccagcggc tgcggattgc cgccgtggaa
      541 atcgacgcgt cgggcctgcc ccggcagacg acggccgacg cgccatgcgt tgcccacctt
      601 gcagggacgc caacgctcgg ctcgccggta ttcgatggcg cgggtcgggg caacgcttgc
      661 gttccagagc gggaagaccg ttcccgcgta ccagcgtgca tcgatctcgt tcgtgcgcgc
      721 aggcgcgtcg agcgtgttca gcatctcgtt cctcgaaggt cctgtccctc tgaagaacgg
      781 aaaatcgacg tgaaacccgc cagccgtccg tcggtcgggt ctcgccagtt gctacagaac
      841 gtaacgcacc gtccggcgtt cgtcgatcga gaccttgccg ctcgcgacga gtcgatcgag
      901 catcgcccgg gcttcgcccg aatcggctgc ggcgccgatc atctgggcga cgtcggcaag
      961 gagtcgcgcc tgcttgtgcg gcagccgatc gacgggccgc ttgcccagca atgccaccac
     1021 ctgctcgaac gactttcccg tgcgcgggct cgccgcgcgc ccgggatcga cgcgcgacgg
     1081 cgattccgcc gcggccgtcg cgggctttcg acggcgtgag ggcgctgccg ctgcactcga
     1141 cgttgcctcg gcctttgcgg tcttgcctgc cgccgggcgg cggatccctt tcgctgccgg
     1201 ggcaggcgcg ggcgtcgcct tcgcggcctt cggggcggac ttcccggccg cgggtgcggg
     1261 cggcgtggac ggccgcgggc gcgtgcgcga actcgcggcc ttcgcagccc gggcatcggt
     1321 ttgcggcgca gcgtcggcac gggtcgactc ctgcgccgat cgcttggccg gggtcttcgt
     1381 cggcgccgat cggcgtgtcg ccgtcgctgc cttcgccgca ccgccggtcg cggcttcgag
     1441 gggaccggtc gccgcgttga cgcgtgtcgg cgccgctgcg gatggacgcg tctttcgcgc
     1501 tgccttcgtg gccgtgccgg cgccgcgggc cttcgccgcg gcggacttcg tcgccgcgcc
     1561 gcggcccttg agccgtacga agccgacctg gtcggcggcg aagccgagct ccttggcgtg
     1621 ctccagcatc gggccgtagc ccttgtcgtt ggagatgacg atgaagcgcg cctcaggttg
     1681 tcgcgacgcg atgtagccga tgtagaaggt gagatggaag tcgagtgcgt tcttgccggt
     1741 gcgggcgatc cgcaccggcg tcgcgcggtc gccgaacgtg gcgtggtgcg ccgcgacgtt
     1801 cttctggttg gggccgtgga agagccacag gtcggtcgca tcggggacga gctcgcggac
     1861 gtcgacgtcg ctcggctgca cgttctccca gtcgatcagc acgtgcgtct tggatccggg
     1921 gctggcgtcg agctcgatcg aggtccggtc ggcgaagctc aacagttcgg ccgcgaaacg
     1981 gcgcaaggtg gcctgtacgg cgggaatgtc catggaaggg gtccttctgg tggcgacgtg
     2041 cctgccgatt agacgcgacc gccgtctcgg cggcacatgg caacattcgc gacccggccg
     2101 acgagccgtc ttcaacgcca cgtgatgcat cgccaatgag cctggcctcc gtgcgcgctt
     2161 ttcttgccga tcgtgcgccc gacatcgaga ttctcgaggc gtcgtcgagc accgccaccg
     2221 tcgccctggc ggccgaagcg catggcgtca cgcccgggca gatcgcgaag acgctgtcgt
     2281 tgcgcctggg cggcgaggcc atcctcgtcg tcatgcgtgg cgacgcgcgg ctcgacaacc
     2341 gcaagttcaa ggatcgcttc ggcgccaagc ccaagatgct cgacgccgcc gaggtcgagc
     2401 aggcgaccgg ccatccggtg ggcggcgtct gtccgttcgg actgccggcg aagcttcgcg
     2461 tttacgccga tgtgtcgctt cgcgccttcg acgtggtcgt cccagcggcc ggcgacacgc
     2521 acacggcgct gcgcgtcgcg ccggtgcggc tggccgagct tgccggcgcc gagtggatcg
     2581 acgtggcaca gccctgacga gccgtgcgcg gcagttgccg ctagacgcgc cgcgcggcgg
     2641 ctggctggta gcttcccggg acc
//
LOCUS       DASRWS010000493         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1066418, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000493 DASRWS010000000
VERSION     DASRWS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066418"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2478
                     /locus_tag="VFF72_09285"
     CDS             <1..2478
                     /locus_tag="VFF72_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZV93399.1"
                     /translation="FGRWWRRQSAARQDRYATLGPLASVLLFLAAIISAFWYLRNEEF
                     EREQDAVKRDTEVAQQQLRLRLIEDDEQLVRVARELATHTLDRDGFLIQASTFTDERP
                     ETTNLIWLSASRAKVASYSGTSFPPETGISGIDTPASLPVANAETEPERAFERARDTR
                     QTVYSKPFSDSFGNPVFQMLIPLVERGAFSGVLVAEYSVERLLRYGVPTEISRRHALS
                     LVDVRTHSLASTVMTLPGTPAARPSITYELPVAPSDNGLVLRGEGYRTSIGLISNTLF
                     WMVMALSVLTVWMLLGTWRHMRRRLQMQAALVSEMNFRRAMENSMLTGMRAMDLEGRI
                     SYVNPAFCVMTGFAEADLIGQRPPFPYWPHGRHEENARLLEQELAGRSPAGGIEVKVM
                     RKDGSLFDARMYVSPLIDPKGHQTGWMTSMTNITEAKRIRDQLTASHERFTTVLEGLD
                     TAVSVVSVQQGELLFANHSYRLWFGADARGHAKLSGGEASHGLPPDSDFGDAVDALGG
                     LPTQELTSAGSDSREVFVESLGKWFDVRARYLQWTDGALVQMLIATDISERRNAEAES
                     ERQAETAQVTSRLVTMGEMASSVAHELNQPLTAISNYCNGMVSRVENDAIDKGDLIAA
                     LQKTSRQAERAGQIIHRIRAFVKRSEPQRQPASAQAIVDDAVDLAGIELRRRNVSIHV
                     YVAQRLPTIMADPILIEQVVLNLLKNAAEAIDNARLPVSRRRIELRVVPRHTPDEGDV
                     IEFTVSDKGPGLKDEVIARMYEAFFSTKVDGLGIGLSLCRSIVESHRGRIKAVNLYNG
                     DTPAGCRFSFTLPAEPTRTEAPAAATA"
     gene            2475..>2809
                     /locus_tag="VFF72_09290"
     CDS             2475..>2809
                     /locus_tag="VFF72_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV93400.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVKCFDSAESFL
                     SRFDPREVACLIADIRMEGMSGLELQDRLLERRSPLPVTFITGHGDVPMAVTTMKKGA
                     MDFIEKPFK"
BASE COUNT          491 a          976 c          905 g          437 t
ORIGIN      
        1 ttcggccgct ggtggcggcg ccagtcggca gcgcgccagg accgctatgc cacgctcggt
       61 ccgctcgcat cggttcttct ctttctcgcg gcgatcatct cggcgttctg gtacctgcgc
      121 aacgaggaat tcgagcgcga gcaggacgcc gtcaagcgcg acaccgaagt cgcccagcaa
      181 caactgcgcc tgcgcctgat cgaggacgac gagcaactcg tccgtgtcgc ccgcgaactc
      241 gcgacacata ccctcgatcg cgatggcttc ctcatccagg cgtcgacctt caccgacgaa
      301 cgacccgaaa cgaccaacct gatctggctg agcgcctcgc gggccaaggt cgcgagctat
      361 tcgggcacga gcttcccgcc ggagacgggc atcagcggca tcgacacgcc ggcgtccttg
      421 ccggtcgcca acgcggagac cgagcccgag cgcgccttcg agcgggcccg cgacacgcgc
      481 cagacggtgt actcgaagcc gttcagcgac agcttcggca atccggtgtt ccagatgctc
      541 attccgctcg tcgagcgcgg cgccttcagc ggcgtgctgg tggccgagta ctcggtcgag
      601 cgactgctgc gctacggcgt gccgaccgag atctcgcgtc gacacgccct ctccctcgtc
      661 gacgtgcgca cgcattcgct cgccagcacg gtgatgacgc tgcccggcac gccggcggcg
      721 cgtccttcga tcacctacga gttgcccgtc gcgccgagcg acaacggcct ggtgctgcgc
      781 ggcgagggct atcgcacatc gatcggtctc atcagcaaca cgctgttctg gatggtgatg
      841 gcgctgtcgg tgctcaccgt ctggatgctg ctcggcacct ggcgacacat gcgacgccgg
      901 ctgcagatgc aggccgcgct ggtctcggag atgaacttcc ggcgcgccat ggagaactcg
      961 atgctgaccg gcatgcgggc catggacctc gaaggccgca tcagctatgt caatccggcg
     1021 ttttgcgtga tgaccggatt cgccgaggcc gacctcatcg gccaacgacc gcccttcccg
     1081 tactggccgc acggccggca cgaggaaaac gcacgattgc tcgagcagga gctcgccggc
     1141 cgcagccctg ccggcggtat cgaggtcaag gtgatgagga aggacggatc gctgttcgac
     1201 gcccgcatgt acgtctcgcc gctgatcgac ccgaaggggc accagaccgg ctggatgacg
     1261 tcgatgacca acatcaccga ggccaagcgg atccgcgacc agctcaccgc atcgcacgag
     1321 cgcttcacga ccgtcctcga aggcctggat accgccgtct cggtggtctc ggtgcagcag
     1381 ggcgagctgc tgttcgcgaa tcactcctac cgtctctggt tcggcgccga cgccaggggt
     1441 cacgccaagc tgtcgggcgg cgaagcgtcg cacgggcttc ctcccgacag cgatttcggc
     1501 gacgcggtcg acgcgctcgg cggcctgccg acccaggagc tcacgtcggc gggctccgat
     1561 tcgcgcgaag tcttcgtcga atcgctcggc aagtggttcg acgtccgcgc ccgctacctg
     1621 cagtggaccg acggggcact ggtgcagatg ctgatcgcca ccgacatctc ggaacgccgc
     1681 aatgccgagg cggaatccga gcgccaggcc gagacggccc aggtgacgag ccggctggtg
     1741 acgatgggcg agatggcatc gtcggtggcg cacgagctca accagccgct gacggcgatt
     1801 tccaactact gcaacggcat ggtcagccgc gtcgagaacg acgcgatcga caagggcgat
     1861 ctcatcgccg cgctgcagaa gacttcgcga caggccgaac gggccggcca gatcatccac
     1921 cgcatccgcg ccttcgtgaa gcgcagcgag ccgcagcgcc agcccgcctc ggcgcaggcc
     1981 atcgtcgacg acgcggtcga cctcgccggc atcgagctgc gccggcgcaa cgtctcgatc
     2041 cacgtctacg tcgcgcagcg gctgccgacg atcatggccg acccgatcct gatcgagcag
     2101 gtcgtcctca acctgctgaa gaacgcggcc gaggccatcg acaacgcccg cctgccggta
     2161 tcgcggcggc gcatcgaatt gcgagtcgtg ccacggcaca cgccggacga aggcgacgtc
     2221 atcgagttca cggtcagcga caaggggccg ggcctgaagg acgaggtcat cgcccggatg
     2281 tacgaggcgt tcttctcgac gaaggtcgac ggcctcggca tcggcctgag cctgtgccgc
     2341 tcgatcgtcg agagccatcg gggcaggatc aaggcggtca acctctacaa tggcgacacc
     2401 cccgccggct gccgtttttc gttcacgctg cccgccgaac caacacgcac cgaagccccc
     2461 gcggccgcca cagcatgagc ctgatcccga agaaaggaac cgtctacgtc gtcgacgacg
     2521 acgaggccgt ccgcgattcg ctgcagtggc tgctcgaggg caaggactac cgcgtcaaat
     2581 gcttcgactc ggccgagtcg ttcctgtcgc gcttcgatcc gcgcgaggtc gcctgcctga
     2641 tcgccgacat ccgcatggag ggcatgagcg gcctcgagct ccaggatcgt ctgctcgagc
     2701 gccgctcgcc gctgccggtg accttcatca ccggtcacgg cgacgtgccg atggccgtga
     2761 cgacgatgaa gaagggcgcg atggacttca tcgagaagcc gttcaagga
//
LOCUS       DASRWS010000494         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3390906, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000494 DASRWS010000000
VERSION     DASRWS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3390906"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFF72_09295"
     CDS             complement(<1..637)
                     /locus_tag="VFF72_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZV93401.1"
                     /translation="MHEPQGAISAPLADASPAAPTGPETVAPSLLFSSVGLSPVLQRA
                     VADQGYTTMTPIQAKAIPLVLAGRDIMGAAQTGTGKTAAFSLPLLHKMLSHENSSMSP
                     ARHPVRALVIAPTRELADQVADNIKGYAAHSKLRVAVVFGGIDMKPQTLELKQGVEVL
                     VATPGRLLDHIEAKNCVLNHVEYVVLDEADRMLDIGFLPDLQRILSYLPKQR"
     gene            703..1779
                     /gene="rfbB"
                     /locus_tag="VFF72_09300"
     CDS             703..1779
                     /gene="rfbB"
                     /locus_tag="VFF72_09300"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZV93402.1"
                     /translation="MILVTGGAGFIGSNFVLDWLAQSDEPVVNLDALTYAGNLENLAT
                     LEGDGRHHFVRGDIVEAALLDRLFDEHRPRAVVHFAAESHVDRSIHGPAAFVRTNVEG
                     TFTLLEAARRHWTQMPSTEREIFRFVHVSTDEVYGSLQPAAPAFAETNTYAPNSPYSA
                     SKAASDHLVRAWHHTYGLPTVTTNCSNNYGPFHFPEKLIPLTIVNALAGKPLPVYGDG
                     QQVRDWLYVKDHCAAIRAVLGRGRPGETYNIGGWNEKPNLEIVHTVCALLDEMAPDAA
                     GSYRRLITHVTDRPGHDRRYAIDARKIEGELGWRPAETFETGLRKTLRWYLDHADWTR
                     RVQTGAYRDWLSANYGSRARAEIE"
     gene            1776..2672
                     /gene="rfbD"
                     /locus_tag="VFF72_09305"
     CDS             1776..2672
                     /gene="rfbD"
                     /locus_tag="VFF72_09305"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZV93403.1"
                     /translation="MKILLFGKNGQVGWELQRALAPIGEVVALDFDSAGPLRADFSEP
                     ASLAATVRAVRPDVIVNAAAHTAVDKAESEPDLAHRLNAEAPGVLAREAAASGAWLVH
                     YSTDYVFDGSGHTARDEGAPTGPLNVYGRTKLEGEEAIRQSGCRHLLFRTSWVYGTRG
                     GNFAKTMLRLAQERSELSVIDDQIGAPTGADLLADVAAHAIRAASTAPSLAGTYHLVA
                     SGETSWHGYASHVIEFARAAGVPIKVAANAIAAVPGTAFPLPAVRPKNSRLDTSRLRQ
                     AFGLTLPPWQAGVDRMLHELLT"
     gene            2683..3576
                     /gene="rfbA"
                     /locus_tag="VFF72_09310"
     CDS             2683..3576
                     /gene="rfbA"
                     /locus_tag="VFF72_09310"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZV93404.1"
                     /translation="MQSAKPRKGIILAGGSGTRLHPATLALSKQLLPVYDKPMVYYPL
                     STLMLAGIREILVISTPQDTPRFEHLLGDGAAWGLSIEYCVQPTPDGLAQAFILGRRF
                     VGGAPSALVLGDNIFHGHDLQRLLQSASARSTGATVFAYHVQDPERYGVVEFDGDRRA
                     VSIEEKPKQPQSNYAVTGLYFYDEQVCDIAADIEPSARGELEITEVNARYLAQGGLTV
                     EILGRGYAWLDTGTHDTLLEASLFISTLEKRQGLKVACPEEIAYRSGWISAQQLETLA
                     RPMLKNGYGQYLQRVLNDRVF"
     gene            3648..4019
                     /locus_tag="VFF72_09315"
     CDS             3648..4019
                     /locus_tag="VFF72_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HZV93405.1"
                     /translation="MDASSPEPSLASLELMRLLEEHPDCSQRELSKRMGVSLGKTHYL
                     LRALLEKGWVKAQNFSRSDSKLRYAYVLTPSGVHERLKLTRSFLARKEREYIELKSQI
                     SVLRKELAQDEASAPTTSGHP"
     gene            4016..>5129
                     /locus_tag="VFF72_09320"
     CDS             4016..>5129
                     /locus_tag="VFF72_09320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZV93406.1"
                     /translation="MKITVIGTGYVGLITGACLADMGNEVLCLDVDSVKVDFLNGGGI
                     PIHEPGLAPLVSNNLQSRRLTFTDDVDRAVAHGDLQFIAVGTPPDEDGSADLRHVLDA
                     ARAIGQRMTGYKVVIDKSTVPVGTAAQVRACIEAELSKRKVEATFSVVSNPEFLKEGA
                     AVDDFMRPDRIVVGAEDERAIALMRALYQPFLRNHDRLLMMDLRSAELTKYAANAMLA
                     TRISFMNELANLAEDLGADIESVRKGIGSDSRIGYSFLYAGCGYGGSCFPKDISALIR
                     TGEERGHALAILRAVEAANESQKGVLARKIVKRFGPDLSNRKFAIWGLSFKPNTDDLR
                     QAPSRFLIDELVARGGEVVAYDPTAMGEARKLYATQP"
BASE COUNT          931 a         1720 c         1656 g          822 t
ORIGIN      
        1 gccgctgctt gggcagatac gacaggatcc gctgcaagtc gggcaggaag ccgatgtcga
       61 gcatgcggtc ggcctcgtcg aggacgacgt actcgacgtg attcaggacg caattcttcg
      121 cttcgatgtg gtcgagcaac cggcccggcg tggccaccaa gacctcgacg ccctgcttca
      181 attcgagcgt ctgcggcttc atgtcgatgc cgccgaagac caccgcgaca cgcagcttcg
      241 agtgggcggc gtaccccttg atgttgtcgg cgacctgatc ggcgagctcg cgcgtcggcg
      301 cgatgaccag cgcccgcacc gggtggcggg cgggcgacat gctgctgttt tcgtgcgaca
      361 gcatcttgtg cagcagcggc agcgagaacg cggccgtctt gccggtgccc gtctgcgccg
      421 cgcccatgat gtcgcgcccg gccaggacga gcggaatcgc cttggcctgg atcggcgtca
      481 tcgtcgtgta gccctggtcg gcgacggcgc gctgcaggac gggggacaag ccgacggacg
      541 agaacagaag cgagggggcg accgtttcgg gaccggtcgg cgcagcgggg ctcgcatcgg
      601 cgagcggcgc ggagatcgcg ccttggggtt catgcatcac gcaattatcg cgcctgcccg
      661 cgcccgccgc agaaagggga gatgcggccg atagaatttc tcatgattct cgtcaccggc
      721 ggggccggtt tcatcggctc caatttcgtt ctcgactggc tggcgcagtc cgacgagccc
      781 gtcgtcaacc tcgacgccct cacctacgcc ggcaatctcg agaatctggc gacgctcgaa
      841 ggcgacggcc ggcaccactt cgtgcggggc gacatcgtcg aggccgcgct cctcgaccgc
      901 ctgttcgacg agcaccggcc gcgcgccgtc gtgcatttcg ccgccgaaag ccacgtcgat
      961 cgaagcatcc acgggccggc cgccttcgtg cgaaccaacg tcgaaggcac cttcacgctc
     1021 ctcgaggcag cgcggcggca ttggacgcaa atgccgtcga cagaacgcga aattttcagg
     1081 ttcgtgcacg tctccaccga cgaggtctac ggctcgctgc aaccggccgc ccctgccttc
     1141 gccgagacga atacctacgc gcccaacagt ccgtattcgg ccagcaaggc ggccagcgat
     1201 cacctcgtgc gcgcctggca ccacacctat ggcttgccga cggtgaccac gaactgcagc
     1261 aacaactacg gaccgttcca tttccccgag aagctgatcc cgctaacgat cgtcaacgcc
     1321 ctggccggca agccgttgcc ggtgtacggc gacgggcagc aggtgcgcga ctggctgtac
     1381 gtgaaggacc attgcgccgc gatccgcgcg gtgctcgggc gcggcaggcc gggcgagacc
     1441 tacaacatcg gtggctggaa cgagaaaccc aacctcgaga tcgtgcatac cgtgtgtgcc
     1501 ctgctcgacg agatggcgcc ggatgctgcg ggctcgtatc gccgcctgat cacccacgtc
     1561 accgatcgcc ctggccatga ccggcgctac gccatcgatg cgcgcaagat cgaaggcgaa
     1621 ctcggctggc ggccggccga gaccttcgag accggcctgc gcaagacgtt gcgctggtac
     1681 ctcgaccacg ccgactggac aagacgcgtg cagaccggcg cctatcgcga ctggctctct
     1741 gcgaattacg gcagccgggc gcgcgccgaa atcgaatgaa gatcctgctg ttcggcaaga
     1801 acggccaggt cggctgggag ctgcagcgag cgctcgcacc aatcggcgag gtagtggcgc
     1861 tcgacttcga cagcgccggg ccgttgcgcg ccgacttctc ggagcctgca tcgctagccg
     1921 ccacggtgcg cgccgtacgg cccgacgtga tcgtcaatgc cgcggcgcac accgcagtcg
     1981 acaaggccga aagcgagccc gatctggcgc atcggctgaa cgccgaagcg cccggcgtgc
     2041 tggcccgcga ggccgccgcc tcgggcgcct ggctcgtcca ctacagcacc gactacgtct
     2101 tcgacggcag cggccacacg gcgcgcgacg agggcgctcc gaccgggccg ctgaatgtct
     2161 acggccggac caagctcgaa ggcgaagagg cgatccggca gagcggctgc cggcaccttc
     2221 tctttcgcac gagctgggtc tacggcacgc gcggcggcaa cttcgccaag acgatgctgc
     2281 gcctggcgca ggaacgcagc gagctgtccg tgatcgacga tcagatcggc gcgcccaccg
     2341 gggccgatct gctggccgac gtcgccgccc atgccattcg tgccgcgagc accgcgccct
     2401 cgctggccgg tacgtaccac ctcgtcgcca gcggcgagac gagctggcac ggctacgcga
     2461 gccacgtcat cgaattcgcc cgtgccgccg gcgtgccgat caaggtggca gccaacgcga
     2521 ttgcagccgt tcccggcacg gcgttcccgc tgcccgcggt gcggccgaag aattcgcggc
     2581 tcgacacgag ccggctgcgc caggccttcg gcctgacact gccgccctgg caagcaggcg
     2641 tcgaccgcat gctgcatgag ctcttgacct gacaggactg cgatgcaaag cgccaagccc
     2701 cgcaagggca tcatcctcgc cggcggctcc ggcacccggc tgcacccggc caccctggcc
     2761 ctcagcaagc aactgctgcc ggtgtacgac aagccgatgg tctattaccc gctctcgacg
     2821 ctgatgctcg ccggcatccg cgagatcctc gtcatcagca cgccgcagga cacgccgcgc
     2881 ttcgagcatc tgctgggcga cggcgcggcc tggggcctgt cgatcgaata ctgcgtgcag
     2941 ccgacccccg atggcctggc gcaggcgttc atcctcggac gccggttcgt cggcggcgcg
     3001 ccgagcgccc tggtgctcgg cgacaacatc tttcatggtc acgacctgca gcgcctgctg
     3061 cagtcagcgt cggcgcgatc caccggtgcg accgtcttcg cctaccacgt acaagacccg
     3121 gagcgatatg gcgtcgtcga gttcgacggc gaccgacgcg cggtcagcat cgaagaaaag
     3181 ccgaaacagc cgcagagcaa ttacgccgtc acgggactct atttctacga cgagcaggtt
     3241 tgcgatatcg cggctgacat cgagccttcg gcacgtggcg agctggaaat caccgaggtc
     3301 aacgcgcgct acctggcaca aggcggcctg acggtggaaa ttctcggccg cggctatgcc
     3361 tggctcgata ccggcacgca cgacacgctg ctcgaggcca gccttttcat ctccaccctc
     3421 gagaagcgac aaggtctgaa ggtggcctgc cccgaagaga tcgcctatcg ctcgggttgg
     3481 atctcggcgc aacagctgga aacgctggcc cggccgatgc tgaagaacgg ctacgggcaa
     3541 tatctgcaac gcgtgctcaa cgaccgcgtc ttctgacgcg cagattaccc gggaggctcg
     3601 tatttcgagc ttgtagaatc cagcgttcaa cgattgaaca ccttgccttg gacgcttcat
     3661 ctccggaacc ctcgctcgcc tcgctcgagc tgatgcggct gctcgaggag caccccgact
     3721 gctcgcagcg cgagctttcg aagcgcatgg gcgtgagcct cggaaaaacg cattacctcc
     3781 tgcgcgccct gctcgaaaaa ggctgggtca aggcgcagaa cttcagccga agcgacagca
     3841 agctgcgcta tgcctatgtg ctgacgccga gcggcgtgca tgagcggctg aagctgacgc
     3901 gatcgttcct ggcgcgcaag gagcgcgaat atatcgagct caagtcgcag atctctgtgc
     3961 tgcgcaagga gcttgcgcaa gacgaggcga gcgcgccaac gacgagcgga catccatgaa
     4021 gatcacggtg atcggcacgg gctacgtggg cctcatcacg ggtgcatgtc tggcagacat
     4081 gggcaatgaa gtgctctgtc tcgacgtcga ctcggtcaag gtcgatttcc tcaacggcgg
     4141 cggcattccc atccacgaac ccggactggc gccattggtt tccaacaacc tgcagtcccg
     4201 caggctgacc ttcaccgacg acgtcgaccg cgccgtcgcc cacggcgacc tgcaattcat
     4261 cgctgtgggc acgccgcccg acgaggacgg gtcggccgat ctgcgacacg tgctggatgc
     4321 ggcacgcgcc attggccaac gcatgaccgg ctacaaggtg gtcatcgaca aaagcaccgt
     4381 gccggtagga accgccgcgc aggtgcgggc gtgcatcgag gcggagctct cgaagcgcaa
     4441 ggtcgaagcg acattctcgg tggtctccaa tcccgagttc ctgaaggagg gagctgcggt
     4501 cgacgacttc atgcggcccg accgcatcgt ggtcggcgcc gaagacgagc gtgccatcgc
     4561 gttgatgcgg gcgctctacc agcctttcct tcgcaaccat gaccgcttgt tgatgatgga
     4621 tctgcgatcg gcggaactga ccaaatacgc ggcgaacgcc atgctggcga ctcgcatcag
     4681 tttcatgaac gaactggcca acctggccga ggatctgggt gccgacatcg aatcggtacg
     4741 caaggggatc ggatcggact cgcggatcgg ctactcgttc ttgtatgcgg gctgcggcta
     4801 tggcggatcg tgctttccga aggacatcag cgcgctcatc cggacgggcg aagagcgcgg
     4861 gcatgcgctt gcgatcctca gagccgtcga ggccgccaac gagtcgcaga agggcgtgtt
     4921 ggcgagaaag atcgtgaagc gcttcgggcc cgacctttcg aacaggaaat tcgcgatatg
     4981 gggcttgtcg ttcaagccca acacggatga cctgcgccag gccccgagcc gcttcctgat
     5041 cgacgaactc gtcgctcgcg gcggcgaggt ggtcgcctac gatccgacgg cgatgggcga
     5101 agcgcgcaag ctctatgcga cacagccgc
//
LOCUS       DASRWS010000495         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1901771, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000495 DASRWS010000000
VERSION     DASRWS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901771"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /gene="otsB"
                     /locus_tag="VFF72_09325"
     CDS             <1..812
                     /gene="otsB"
                     /locus_tag="VFF72_09325"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZV93407.1"
                     /translation="ARSFGRPALSMPRHLFSREGEDALAATLALRPLLAFDFDGTLAP
                     IVARPEDARVPLPVARRLDQLARLLPVAIVSGRRIDDVRGRLSFEPHHIIGSHGAEDP
                     LDPQSDTAPARLDAFRSRLRRRAADLDAAGVVVEDKAHSLALHYRLARDRERARSLVD
                     ELVSGPDAGLHTFGGKMVMNVVAAGARDKADAVASLVERFGVRAAIFLGDDVNDEPVF
                     ARAEPAWLTIKVGRDDPASAARFCIDGTGEIARLLDRMLALLGDPGVAPPR"
     gene            815..1342
                     /locus_tag="VFF72_09330"
     CDS             815..1342
                     /locus_tag="VFF72_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZV93408.1"
                     /translation="MASQPSPRVPAEPAVRASTTQAGEDASVRVLRRFRLVFNAVKSH
                     FQHIEKASGIGGAQVWALSIIGERPGLGVTDLARAMDVHQSTASNLVRSLVERGLVDT
                     TRAGPDRRAVQLRLLAAGRRVLRRAPPPLTGVLPQALARLDEATLRRLDHDLAELIAV
                     LDTDHDAAHQPLAHL"
     gene            1361..>2094
                     /locus_tag="VFF72_09335"
     CDS             1361..>2094
                     /locus_tag="VFF72_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HZV93409.1"
                     /translation="MPSPIEEYALIGDCHTAALVGKDGSIDWMCMPRFDSPACFAALL
                     GSPEHGRWRIAPAGKTVRTTRRYRGDTMILETDFETDTGVVRIIDCMPLDTERWDVLR
                     IVEGRSGRVAMAMELVIRFDYGSIVPWVRSSEEGVFAIAGPDTLELRTPVETRGESMT
                     TVAEFEVGAGDRVPFVLTYRLSHETPQAAIDAEAELEQTEQRWLEWSSRCSAPPRWRA
                     AVMRSLLTLKALTYTATGGIVAAPTTS"
BASE COUNT          276 a          793 c          729 g          296 t
ORIGIN      
        1 tggcgcgttc gttcggccgg cccgcactga gcatgccgcg ccacctcttt tcccgcgaag
       61 gcgaggacgc gctggccgcg acgctggcgt tgcggcctct cctcgcgttc gacttcgacg
      121 gcacgctggc gcccatcgtc gcccggcccg aagacgcgcg cgtgccgctg cccgtcgcgc
      181 ggcgcctcga ccagctcgcc cggttgctgc cggtggccat cgtcagcggc cgccgtatcg
      241 acgacgtgcg cggccggctg tcgttcgagc cgcatcacat catcggcagc cacggcgccg
      301 aggatccgct cgacccgcaa agcgacaccg cgccggcacg cctcgatgcg tttcgctcgc
      361 gcctgcgacg gcgcgctgcc gatctcgacg ccgcgggcgt cgtcgtcgag gacaaggcgc
      421 actcgctggc gctgcactac cggctggcgc gcgatcgcga gcgggcccgc agcctcgtcg
      481 acgagctggt ctcgggcccc gatgccgggc tgcacacctt cggcggcaag atggtgatga
      541 acgtcgtcgc cgccggcgcc cgcgacaagg ccgacgccgt ggcgagcctg gtcgagcgct
      601 tcggcgtgcg cgctgccatc ttcctcggcg acgacgtcaa cgacgagccg gtcttcgccc
      661 gcgccgagcc cgcctggctg acgatcaagg tcggccgcga cgacccggcg agcgccgctc
      721 gcttctgcat cgacggaacg ggcgagatcg cgcgtctgct cgatcgcatg ctggcgctgc
      781 tcggcgatcc gggcgtggcg ccgccgcgct gaagatggcg agccagccgt cgccgcgcgt
      841 gccggccgag ccggccgtca gggcgagcac gacgcaggca ggcgaagacg cgtcggtgcg
      901 cgtcctgcgc cgcttcaggc tcgtcttcaa tgccgtgaag tcgcacttcc agcacatcga
      961 gaaggcgtcg ggaatcggcg gcgctcaggt ctgggcgctc agcatcatcg gcgagcggcc
     1021 cggcctcggc gtcaccgacc tggcccgggc catggacgtg catcagtcga ccgccagcaa
     1081 tctcgtccgc tcgctggtcg agcgcgggct ggtcgacacg acgcgcgcgg ggcccgatcg
     1141 gcgcgccgtg cagttgcgcc tgctggcggc ggggcgccgc gtgctgcgtc gcgcgccgcc
     1201 gccgctcacg ggcgtgctgc cgcaggcgct ggcgcgtctc gacgaagcga ccttgcgccg
     1261 cctcgaccac gaccttgccg agctgatcgc cgtgctcgac accgaccacg acgcggcaca
     1321 ccagccgctg gcccatctct agactcgacc cggaggccgc atgccctcgc ccatcgaaga
     1381 gtacgccctg atcggcgatt gccacaccgc cgcgctggtc ggcaaggacg gatcgatcga
     1441 ctggatgtgc atgccgcgct tcgattcgcc ggcctgcttc gccgccctgc tcggctcgcc
     1501 cgagcacggc cgctggcgca tcgcgccggc cggcaagacg gtgcgcacca cgcgccgcta
     1561 ccgcggcgac acgatgatcc tcgagaccga cttcgagacc gacaccgggg tcgtccgcat
     1621 catcgactgc atgccgctcg acaccgagcg ctgggacgtg ctgcgcatcg tcgaagggcg
     1681 cagcggccgt gtcgcgatgg cgatggagct cgtcatccgc ttcgactacg gctcgatcgt
     1741 cccctgggtg cgcagcagcg aggaaggcgt cttcgcgatc gccggccccg acacgctcga
     1801 gctgcgtacg ccggtcgaga cgcgcggcga gtcgatgacg acggtggccg agttcgaggt
     1861 cggcgccggc gaccgcgtcc cattcgtcct cacctatcgc ctctcgcacg agacgccgca
     1921 ggcggcgatc gacgccgagg ccgagctcga gcaaacggag cagcgctggc tcgaatggtc
     1981 gtcgcgctgc agcgcgccgc cgcgctggcg ggccgccgtg atgcgctcgc tcctgacgct
     2041 gaaggcgctc acctacaccg cgaccggtgg catcgtcgcc gcgccgacga cctc
//
LOCUS       DASRWS010000496         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3391325, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000496 DASRWS010000000
VERSION     DASRWS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391325"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VFF72_09340"
     CDS             complement(<1..885)
                     /locus_tag="VFF72_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZV93410.1"
                     /translation="MTRSLALQRELGPVFDVTLDCEDGAPIGGEAEHAHLVAEMATSS
                     ANAFGRCGVRVHPVGDVTFDSDVSIIVGGAAKRLAYIMLPKVAGVADIDRAAATIDAS
                     GGSAIPLHALIETHDGLRAVAAIAAHPRIESLSFGLMDFVSAHRGAIPASAMSAEGQF
                     AHPLVVRAKLEVAAACHAFGRTPSHSVVTEFGDAAVVAAAARRAAREFAYTRMWSIHP
                     GQIRPIVAAFAPDPGEIDEAVAVLQAAADAGWAPVRRGDVLHDRASYRYWWRIAERAM
                     RTGTTLPEAFRRQFSRPGR"
     gene            complement(1023..1796)
                     /gene="tam"
                     /locus_tag="VFF72_09345"
     CDS             complement(1023..1796)
                     /gene="tam"
                     /locus_tag="VFF72_09345"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HZV93411.1"
                     /translation="MADWDPQLYRRFEGERTRPARELLARVDLASPGLVFDLGCGPGN
                     STELLVERWPGARVRGVDNSEAMLAAARQRLPAVEFELSDIGGWRPERAPDLIYANAA
                     LQWVGEHRSLLPRLLAALAPGGSLAVQMPDNLDMPSHRAMREVAALPEFAAAIGDAAA
                     VRSRILPAETYYDLVSNDAAAVDVWRTTYYHPMPSPRAIVDWLRGTGLRPFLQRLDDA
                     ASDTFLREYEARVDAAFPERVDGLRLLPFPRLFIVATRR"
     gene            complement(1812..>2604)
                     /locus_tag="VFF72_09350"
     CDS             complement(1812..>2604)
                     /locus_tag="VFF72_09350"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZV93412.1"
                     /translation="PLLAGVVATDNAEVAFKDADFALLVGARPRGPGMERKDLLSANA
                     QIFTAQGKALDKVASRNVKVLVVGNPANTNAYIAMKSAPGLPRRNFTAMLRLDHNRAA
                     SQIAAKTGKPVASIEKLAVWGNHSPTMYADYRFATIDGKSVKQMINDDAWNRDTFLPT
                     VGKRGAAIIEARGLSSAASAANAAIDHMRDWALGSNGRWVTMGIASDGQYGIPKDTMF
                     GFPVTTAGGEYKLVEGLDIDPFSRERIDLTLKELQEEQEGVKHLL"
BASE COUNT          388 a          885 c          937 g          394 t
ORIGIN      
        1 ccttccgggc cgggaaaatt gccgccggaa cgcttcgggc aaggtcgttc cggtgcgcat
       61 cgcccgctcg gcgatgcgcc accagtagcg atagctggcc cggtcgtgca gcacgtcgcc
      121 gcgtcgcacc ggcgcccagc ccgcgtcggc cgctgcctgc agcaccgcaa ccgcctcgtc
      181 gatctcgccc ggatcgggcg cgaaggcggc gacgatggga cggatctggc cgggatggat
      241 gctccacatc cgcgtgtagg cgaactcgcg agcagcgcgt cgcgccgcag cggcaaccac
      301 cgcggcgtcg ccgaattcgg tgacgacgct gtgcgacggc gtccggccga aggcgtggca
      361 ggccgcggcg acctcgagct tggcgcgaac gacaagcgga tgcgcgaact ggccttcggc
      421 cgacatcgcc gaggccggga tcgcgcctcg atgcgccgag acgaagtcca tcaggccgaa
      481 cgacagcgac tcgatgcgcg ggtgcgcggc gatcgcggcg accgcccgca gcccgtcgtg
      541 cgtctcgatg agcgcgtgca gcgggatcgc cgacccgcca gaggcgtcga tcgttgcagc
      601 ggcgcgatcg atgtcggcga cgcccgcgac tttcggcagc atgatgtagg cgagccgttt
      661 cgcggcgccg ccgacgatga tcgagacatc gctgtcgaac gtgacgtcgc cgaccggatg
      721 gacgcggacc ccgcagcggc cgaacgcgtt cgccgacgac gtcgccatct ccgcgacgag
      781 atgggcgtgc tcggcctcgc cgccgatcgg cgcaccgtct tcgcagtcga gcgtgacgtc
      841 gaagaccggc ccgagctccc gctggagggc caggctcctg gtcatccgcg tctcgatgcc
      901 ggcgtagtga tcgcagaccg gaagggtgcg gcaatcgctg gcggcgtcga agagaaacga
      961 cgccggatcg atcacggcgg tttccgccgc gagggctgct tcggatgccg cgatgctgct
     1021 catcaccggc gcgtggcgac gatgaaaagg cgcgggaaag gcagcaggcg aaggccgtcg
     1081 acgcgctctg gaaaggcggc gtcgacgcgt gcttcgtatt cgcgcaggaa ggtgtccgac
     1141 gccgcgtcgt ccagacgctg caggaacggc ctcagtcccg ttccgcgtag ccagtcgacg
     1201 atggcacggg gagacggcat cgggtggtag tacgtggttc gccatacgtc gacggcggcg
     1261 gcgtcgttcg acacgaggtc gtagtacgtc tcggcgggaa ggatccggct gcgcacggcc
     1321 gcggcatcgc cgatcgccgc ggcgaactcg ggcagcgcgg cgacttcgcg catggcgcgg
     1381 tgactcggca tgtcgaggtt gtcgggcatc tgcacggcga gcgatccgcc gggcgcgagc
     1441 gcggcgagga gccgcggcag caggctgcgg tgctcgccga cccactgcag cgccgcattg
     1501 gcgtagatca gatcgggcgc tcgctccggc cgccagccgc cgatgtcgga caactcgaac
     1561 tcgacggccg gcagccgctg ccgcgccgcc gccagcatcg cctccgagtt gtcgacaccg
     1621 cggacacgag cgcccggcca gcgctcgacg agcaactccg tcgagttgcc ggggccgcag
     1681 ccgagatcga agaccaggcc gggcgatgcg aggtccacgc gggcgagcag ctcacgcgcg
     1741 ggtcgcgtcc gctcgccttc gaagcggcga tagagctgcg gatcccagtc ggccatcgcc
     1801 gtgtctgtcg atcagagcag gtgcttgacg ccttcctgct cttcctgcag ttccttcagc
     1861 gtcaggtcga tccgctcgcg gctgaagggg tcgatgtcca gcccctcgac gagcttgtat
     1921 tcgccgccgg cggtggtcac gggaaagccg aacatggtgt ccttcggaat gccgtactgg
     1981 ccatcggagg cgatgcccat cgtcacccac cgcccgttcg atccgagcgc ccagtcgcgc
     2041 atgtggtcga tcgccgcgtt ggccgccgat gccgccgacg acagcccgcg cgcctcgatg
     2101 atcgccgcgc cgcgcttgcc gaccgtgggc aggaaggtgt cgcggttcca ggcgtcgtcg
     2161 ttgatcatct gcttgaccga cttgccatcg atcgtcgcga agcggtaatc ggcatacatc
     2221 gtcggcgagt gattgcccca gacggcgagc ttctcgatcg aggccaccgg cttgcccgtc
     2281 ttggcggcga tctggctggc ggcgcggttg tgatccaggc gcagcatcgc cgtgaagttc
     2341 cttcgcggca ggcccggcgc gctcttcatc gcgatgtagg cgttggtgtt ggcgggattg
     2401 ccgacgacca gcaccttgac gttgcggctg gccaccttgt cgagcgcctt gccctgggcg
     2461 gtgaagatct gcgcgttggc ggagagaagg tccttgcgct ccatgccggg gccgcgcggg
     2521 cgcgcgccca cgagcagcgc gaagtcggcg tccttgaacg ccacctcggc gttgtcggtg
     2581 gccaccacgc cggccagcag cggg
//
LOCUS       DASRWS010000497         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1655675, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000497 DASRWS010000000
VERSION     DASRWS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655675"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="VFF72_09355"
     CDS             complement(<1..2270)
                     /locus_tag="VFF72_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZV93413.1"
                     /translation="MPTPANPTALAQQARRSYVERLLGGIPAVVQAVDQGARTLAGSV
                     AEPAVAMKRREIIPVLQTTFPLWQQGMTTLLRATLETGTVTQTRLGDLPVHAGGQRAG
                     AKLSLVDDDTIEHEILSSRLALAMMDRASWEFTDLRSRMNVLEKREELDTNDILRPHV
                     LARVVTNSWLAAPLGFDAWRLLQSVLHDEFSHFAEEAYHETNRFLLQRHVLPEVDLRP
                     FIRRSNHGGAPAPAILGRSSGSTGGFGTPTGTAASGASDFQYSVMKEAKEPHEVGEET
                     RMMTRASGLARSADQADAVLNRLNRVVGRQLPDFANTSQAHAASPRLSAAIAEAQEGV
                     ARRLATASAAAASAGAPVVGTPALLEELHQRKQALKAAATTPVERATIEIVALLFQSI
                     LMEDAIPATVRVWFARLQMPVLRVAVSEPDFFATVEHPARRLIDRMGSCVMGFDATSR
                     VVGDILEKEIKRVVQVVEAYPDTGRRVFQTVLTEFEKFLEHYFKNENEASKKGVSLAA
                     QVEQRETMAIQYTIELRKMLNDVPVQEGVRDFLFHVWADVLATTAVKAGNADATKEMK
                     RVAADLIWSASAKVSRAERAEVIRRLPPLLKTLRDGMDEAGIPVPKQDEHIQRLNNSL
                     AAAFTAKAAAISHERLEELMQRLETLEELLPEAEQVEIDESLVMDLSGHESSELEVVG
                     EGGSMPTPAMLQWARELQVGSWYMLDYRNRNEAVQLAWQGMRKQLTLFVSPQGRGVLF
                     QQHRLAAFLQAGLLVPA"
     gene            complement(2312..3340)
                     /gene="secF"
                     /locus_tag="VFF72_09360"
     CDS             complement(2312..3340)
                     /gene="secF"
                     /locus_tag="VFF72_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZV93414.1"
                     /translation="MEFFRIRRDIPFMRHALVFNVISALTFLAAVFFLATRGLHYSIE
                     FTGGNVIEVAYAQTADIPKVRDVVEGLGLGDVVVQNFGSSRDVMIRVPVRADIKQGEL
                     TNRVFGALCTAEKGTVKEGVAGQGQSASRQLCAVGANEPLRLSSTEVVGPQVGKELAQ
                     DGAKALAFVVVGIMIYLAFRFEWKFSVAAIVANLHDVVIILGFFAFFQWEFSLSVLAA
                     VLAVLGYSVNESVVIFDRIRESFRRYRKLSTEETIDHAITSTISRTIITHGSTQMMVL
                     SMLAFGGPTLHYFAIALTIGILFGIYSSVFVAAAIAMWLGVKREDLVGSSVAKAHDPK
                     DPNAGAVV"
     gene            complement(3375..>3657)
                     /locus_tag="VFF72_09365"
     CDS             complement(3375..>3657)
                     /locus_tag="VFF72_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZV93415.1"
                     /translation="PQAAINTGYERAFATILDSNVTTLIAGLALLAFGSGPVRGFAIV
                     HCLGIMTSMFSAVLFSRGLVNLWYGRQKRLKSVSIGQVWKPTQSVPAKG"
BASE COUNT          581 a         1247 c         1244 g          585 t
ORIGIN      
        1 gccggcacca gcaggccggc ctgcaggaag gcggcgagcc ggtgctgctg gaacaacacg
       61 ccgcgcccct gcggcgagac gaacagcgtc agctgcttgc gcattccctg ccaggccagc
      121 tgcacggctt cgttgcggtt gcggtagtcg agcatgtacc agctgccgac ctggagctcg
      181 cgcgcccatt gcagcatcgc cggggtcggc atcgagccgc cctcgcccac cacctcgagc
      241 tccgagcttt cgtggcccga caggtccatc accagcgact cgtcgatctc gacctgctcg
      301 gcctccggaa gcaactcctc gagcgtctcg aggcgttgca tcagctcctc gagccgctcg
      361 tgcgagatcg ccgccgcctt ggcggtgaat gccgcggcga gcgaattgtt gaggcgctgg
      421 atgtgttcgt cctgcttggg cacggggatg ccggcttcgt ccatgccgtc gcgaagcgtc
      481 ttcagcaggg gcggcagccg ccgtatcacc tcggcccgct cggccctcga caccttggcg
      541 ctggccgacc agatcaggtc ggcggcgacg cgcttcatct ccttggtcgc gtcggcgttg
      601 ccggccttga cggccgtcgt cgcgaggacg tcggcccaga cgtggaacag gaagtcgcgc
      661 acgccttcct gcaccggcac gtcgttgagc atcttgcgca gctcgatcgt gtactggatc
      721 gccatcgtct cgcgctgctc gacctgcgcc gccagcgaga cacccttctt cgaggcctcg
      781 ttctcgttct tgaagtagtg ctcgaggaac ttctcgaact cggtgagcac cgtctggaag
      841 acgcgccggc cggtgtcggg atacgcctcg acgacctgga cgacgcgctt gatttccttt
      901 tcgaggatgt cgccgacgac gcggctggtc gcgtcgaagc ccatcacgca cgagcccatg
      961 cgatcgatca ggcggcgcgc cggatgctcg acggtggcga agaaatcggg ctcggagacg
     1021 gcgacgcgca gcaccggcat ctgcagccgg gcgaaccaga cccgcaccgt cgccggaatc
     1081 gcgtcttcca tcaggatgct ctggaacagc agggcgacga tctcgatcgt ggcgcgctcg
     1141 acgggtgtcg tggccgccgc cttcagcgcc tgctttctct ggtgcagctc ctcgagcagc
     1201 gccggcgtgc cgaccacagg agcgccggcg ctcgccgctg ccgccgaggc cgtcgcgaga
     1261 cgccgcgcga cgccttcctg cgcttcggcg atcgccgcgc tcaggcgggg cgaagccgcg
     1321 tgggcctgcg acgtgttggc gaaatcgggc aactgccggc cgacgacgcg gttcaaccgg
     1381 ttgagcaccg catcggcctg gtcggcgctg cgcgccaggc ccgaggcgcg cgtcatcatc
     1441 cgcgtctcct cgccgacctc gtgcggctcc ttggcctcct tcatcaccga gtactggaag
     1501 tcggacgcgc cgctggccgc cgttccggtg ggcgtgccga agccgcccgt cgatcccgac
     1561 gagcggccga ggatcgccgg cgccggcgcg ccgccgtggt tcgagcggcg gatgaagggc
     1621 ctcaggtcga cttctggcag cacatggcgc tgcaggagga agcgattcgt ctcgtggtac
     1681 gcctcttcgg cgaaatgcga gaactcgtcg tgcagcaccg actgcaggag tcgccaggca
     1741 tcgaagccga gcggtgccgc cagccacgaa ttggtgacga cgcgggccag cacgtgcggg
     1801 cgcaggatgt cgttggtgtc gagctcctcg cgcttctcga gcacgttcat ccgcgagcgc
     1861 aggtcggtga actcccacga cgcccggtcc atcatcgcca gcgccaggcg cgagctgagg
     1921 atctcgtgct cgatggtgtc gtcgtcgacg aggctcagct tggcaccggc acgctggccg
     1981 ccggcgtgga ccggcaagtc gccgagccgc gtctgggtga ccgtgccggt ctcgagcgtc
     2041 gcgcgcagca aggtcgtcat gccttgctgc cagaggggaa acgtcgtctg caggaccggg
     2101 atgatctcgc ggcgcttcat cgccaccgcg ggttcggcca ccgagccggc cagcgtccgg
     2161 gcaccctggt cgaccgcctg cacgacggcc ggaatgcccc cgagcagccg ctccacatat
     2221 gaccgcctcg cctgctgcgc cagtgccgtc gggttggcgg gggtgggcat ggggtgggtt
     2281 gcgatccgtc ctgcaggaat ctcgttcgac tctacaccac ggccccggcg ttcgggtcct
     2341 tcggatcgtg cgccttggcg accgacgagc cgaccagatc ctcgcgcttg acgcccagcc
     2401 acatcgcgat cgcggcggcg acgaaaaccg acgaatagat gccgaacagg atgccgatcg
     2461 tcagcgcgat cgcgaagtaa tgcagcgtcg ggccgccgaa ggcgagcatc gacaacacca
     2521 tcatctgcgt cgagccgtgg gtgatgatgg tgcggctgat cgtgctcgtg atcgcgtggt
     2581 cgatcgtctc ctcggtcgac agcttgcggt agcggcggaa cgactcgcgg atccggtcga
     2641 agatgacgac cgattcgttc accgagtagc cgagcacggc gagcaccgcc gccagcaccg
     2701 agagcgagaa ctcccactgg aagaacgcga agaagcccag gatgatgacg acgtcgtgca
     2761 ggttggcgac gatggcggcg accgagaact tccactcgaa gcgaaacgcc aggtagatca
     2821 tgatgccgac gacgacgaag gcgagcgcct tggcgccgtc ctgcgccagc tccttgccga
     2881 cctgcggccc caccacctcg gtgctcgaca ggcgcagcgg ctcgttcgcc cccaccgcgc
     2941 acagctggcg cgacgccgac tggccctggc cggcgacgcc ttccttgacc gtgcccttct
     3001 ccgccgtgca gagggcgccg aagacccggt tggtgagctc gccctgcttg atgtcggcac
     3061 gcaccggcac gcggatcatc acgtcgcgcg agctgccgaa gttctgcacg acgacgtcgc
     3121 cgaggcccag gccctcgacc acgtcgcgga ccttcgggat gtcggccgtc tgcgcgtagg
     3181 ccacctcgat cacgttgccg ccggtgaact cgatcgaata gtgcaggccg cgggtcgcca
     3241 ggaagaacac ggccgccagg aaggtcaacg ccgagatgac gttgaagacc agcgcatgcc
     3301 gcatgaacgg gatgtcgcgc cggatgcgga agaactccat cgaatgcttc tctcttgctg
     3361 gttggcggcg gcgctcagcc cttggcaggg acgctctggg tcggcttcca cacctggccg
     3421 atcgagacgc tcttgagacg cttctgccgg ccgtaccaga ggttgacgag accacgcgag
     3481 aacagcacgg cggagaacat cgaggtcatg atgcccaggc agtggacgat ggcgaagccg
     3541 cgcaccggcc cggagccgaa cgcgagcagg gcgaggccgg cgatcagcgt cgtcacgttc
     3601 gaatcgagga tggtggcgaa ggcgcgctcg tagccggtgt tgatcgcggc ctgtggc
//
LOCUS       DASRWS010000498         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3082543, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000498 DASRWS010000000
VERSION     DASRWS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082543"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFF72_09370"
     CDS             <1..292
                     /locus_tag="VFF72_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZV93416.1"
                     /translation="GLLRGYFHGADDDTPDELQQARLLSVTLPPTSASIARSLAEHEL
                     AAAGVDVVSLRRASGAVVGPSPSLALAAGDTLVLSGLPEPLARAEEMLLRSR"
     gene            complement(310..777)
                     /locus_tag="VFF72_09375"
     CDS             complement(310..777)
                     /locus_tag="VFF72_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZV93417.1"
                     /translation="MEIDELVTAVQTLNAEDSFRARLSLENWRVLAPYLARYEIRAGD
                     LLIKQGDADRAMYFIAQGSMQVFVTGGPPGSHRVAILRAGAVVGEPGLFGDTPRTANV
                     EAMTPCVVWALRGPRMEELAQRSPALALELLRAAGAVMAIRFRATLARQAPMT"
     gene            1018..2544
                     /locus_tag="VFF72_09380"
     CDS             1018..2544
                     /locus_tag="VFF72_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZV93418.1"
                     /translation="MDPTPDPFLAMRRELTDWRQWLTRFVVLAAAAAAGLVIVAFTWL
                     ADHALTLFDRIAHLGWWAPLLWMPLSTAGIVWVTRRFAPATAGSGIPQVMASLDTGVA
                     PADTHKFVSLRLTFAKILLTAWGLLAGLSLGREGPSVQIAAGVMYSARKLLPSRSAIS
                     GHGLLVAGGAAGIAAVFNAPLAGVMFAIEELSRRPEQRSSGLLIAAIVLSGLIAVSIY
                     GNATYFGVIQVPSIGWALFWPGLLVTMVCGASGGLFSRLLLRSLSGSGERLNRWRRRR
                     PVLFAAACGGAVALIGLATAGATFGSGYSYTRALLDGTHNAPVIYALCKFTATWITAW
                     SGIPGGLFAPALAIGAGLGNDVAALAGPPERTALIALGMVGFLAAVTQAPLTAFIIVM
                     EMIDGHAMVLSLMACALLSSLFSKLIGPPIYPSLAQLLVPAEPATAPAGATAPVVHEP
                     VVPAPVAAAPPAPPVASTSASIPAASSPPSPVALPAIPSPTADSTTPDDPAAAAQGPA
                     "
     gene            2658..>3293
                     /locus_tag="VFF72_09385"
     CDS             2658..>3293
                     /locus_tag="VFF72_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZV93419.1"
                     /translation="MTRNLVRAAIVVAAAVSFATFAFAADFINVLTGGTSGVYYPMGV
                     ALSQIFAKALPDAKVSVQATKASAENLNLLQAGRGEIAFTLGDSLSDAWKGDAEAGFA
                     TPLKKLRGVAGIYPNYIQIVASADSGIKTLADLKGKRVSVGAPKSGTELNARAVLKAA
                     GITYKDLGRVEYLPFGESVELMKNRQLDVTLQSAGLGVASLRDLASAMKIVI"
BASE COUNT          446 a         1202 c         1102 g          543 t
ORIGIN      
        1 cgggttgctg cgcggctact tccatggcgc cgacgacgac acgccggacg agttgcagca
       61 ggcgcgcctg ctcagcgtca cgctgccgcc gaccagtgca agcatcgcgc gaagcctcgc
      121 cgagcatgag ctcgccgcgg cgggcgtcga cgttgtttcg ctgcgtcgcg cctccggcgc
      181 ggtggtgggg ccgtcgcctt cgctggccct cgccgcgggc gatacgctgg tgctgtccgg
      241 ccttcccgag ccgctcgccc gcgccgagga gatgttgctg cgatcgcgct gaggcgttgc
      301 cgggtcagct caagtcatcg gcgcctggcg cgccagcgtc gcccggaagc gaatcgccat
      361 gacagcgccg gcggcgcgca gcaactcgag cgccagcgcc ggcgaacgct gcgcgagctc
      421 ttccatgcgc ggtccccgca aggcccagac gacgcagggc gtcatcgcct cgacattcgc
      481 cgttcgcggc gtgtcgccga agaggccggg ttcgccgacg accgcgccgg cacgcaggat
      541 ggcgacgcgg tggctgcccg gtggtccgcc ggtgacgaag acctgcatgc tgccctgggc
      601 gatgaaatac atcgcccgat cggcgtctcc ctgcttgatg agaaggtctc cggcacggat
      661 ctcgtagcga gccaggtagg gcgcgagaac gcgccagttc tcgagcgaca gacgcgctcg
      721 aaacgagtct tccgcgttga gtgtctgtac tgctgtgacg agttcgtcga tttccatgga
      781 tcgccttcgt cgccacggcg agccgaagta gcgggcaccg cgcgacggcg gcattatcgg
      841 ccgggcgatg ctggcacgac agccgcctgc gcggccaaac gcaccctttc gtcacgcttg
      901 tggcgccggc agaaccggtc accctgtccg acgcgacctt gctctcgaag ggtgccgtgc
      961 gatcattggc cgctcccgca gtgacgcgcc cgaggcgggc cgcctttttt ccgaacgatg
     1021 gacccgacgc ccgatccctt cctcgcgatg cgccgcgagc tcaccgactg gcggcaatgg
     1081 ctgacccggt tcgtcgttct cgccgccgcg gcggcagccg gcctcgtcat cgtcgccttc
     1141 acctggctcg ccgatcacgc gctcaccttg ttcgatcgca tcgcgcatct cggctggtgg
     1201 gcgccgctgc tctggatgcc gctcagcacg gcgggcatcg tctgggtgac gcgccgtttc
     1261 gcgccggcga cggccggctc gggcattccg caggtgatgg cctcgctcga tacgggcgtc
     1321 gcgccggccg atacccacaa gttcgtgtcg ctccggctca ccttcgccaa gatcctcctc
     1381 acggcatggg gactgctcgc gggcctctcg ctcggccgcg aaggtccctc ggtgcagatc
     1441 gccgcgggtg tgatgtacag cgcgagaaag ctgctgcctt cgcgcagcgc gatcagcggt
     1501 cacggactgc tcgtcgccgg cggcgccgcc ggcatcgccg ccgtcttcaa tgcaccgctg
     1561 gccggcgtga tgttcgccat cgaagagctg tcgcgccggc ccgagcagcg ctcgagcggc
     1621 ctgctgatcg ccgccatcgt cctctccggc ctgatcgccg tgtcgatcta cggcaacgcg
     1681 acgtacttcg gcgtcatcca ggtgccgtcg atcggctggg cgcttttctg gccgggcctg
     1741 ctcgtcacga tggtctgcgg cgcctcgggc gggctcttct cgcgcctgct cctgcgctcg
     1801 ctgagcggca gcggcgagcg actcaaccgg tggcggcggc ggcggccggt gctgttcgca
     1861 gcggcgtgcg gcggcgccgt cgcgctgatc ggccttgcca ccgccggcgc caccttcggc
     1921 agcggctaca gctacacgcg tgcgctgctc gacggcacgc acaacgcgcc cgtgatctac
     1981 gcgctctgca agttcacggc gacctggatc accgcctgga gcggcattcc tggcggcctc
     2041 ttcgcgccgg cgctggccat cggcgcaggc ctcggcaacg acgtcgcggc gctcgccggg
     2101 ccgcccgagc gcacggcact gatcgccctc ggcatggtcg gattcctggc cgcagtgacg
     2161 caggcgccgc tcaccgcatt catcatcgtc atggagatga tcgacggcca tgcgatggtg
     2221 ctgagcctga tggcgtgcgc acttctttcg agcctgttct ccaagctgat cgggccgccg
     2281 atctatccgt cgctcgcgca gttgctggtg ccggccgagc cggctacggc gcctgcgggg
     2341 gcaactgcgc ccgtcgtgca cgagccggtc gtgcctgcgc cggtcgcggc tgcgccacct
     2401 gctccgccgg tcgcatcgac gtccgcgtcg atacctgccg cgtcctcgcc gccgtcgccg
     2461 gtcgctctcc cggccatccc gtcgccgacc gcggactcga cgactcctga cgaccccgcc
     2521 gccgcggcgc aaggcccggc gtaggcgaaa acaccgggcg cgaaacccgc gcgcatgatg
     2581 gtagaacgcg ggaccgcgtc ccggaccctc gcccgcggcg agcggcaacc cggccttgtc
     2641 ctgtccacgg agatcccatg actcgcaacc tcgttcgagc cgccatcgtc gtcgccgccg
     2701 ccgtttcctt cgcgacattc gccttcgccg ccgacttcat caatgtcctc acgggtggaa
     2761 ccagcggggt gtattacccg atgggcgtcg ccctcagcca gatcttcgcc aaggcgctgc
     2821 ctgacgcgaa ggtgtcggtc caggcgacca aggcgtcggc cgaaaacctc aacctgctgc
     2881 aagccggtcg cggcgagatc gccttcacgc tcggcgactc gttgtcggac gcctggaagg
     2941 gtgacgccga ggcgggcttt gcgacgccgc tcaagaagct gcgcggcgtg gcaggcatct
     3001 acccgaacta catccagatc gtggccagcg ccgattccgg catcaagacg ctcgccgatc
     3061 tgaagggcaa gcgcgtctcg gtcggcgcgc cgaaatcggg caccgagctc aacgcccggg
     3121 ccgtcctgaa ggccgcgggc atcacctaca aggacctcgg ccgggtcgaa tacctgccgt
     3181 tcggcgaatc ggtggagctc atgaagaacc ggcagctcga cgtcacgctg cagtcggccg
     3241 gactgggcgt cgcctcgctg cgcgacctcg cctcggcgat gaagatcgtc atc
//
LOCUS       DASRWS010000499         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3941292, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000499 DASRWS010000000
VERSION     DASRWS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3941292"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2269)
                     /locus_tag="VFF72_09390"
     CDS             complement(<1..>2269)
                     /locus_tag="VFF72_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZV93420.1"
                     /translation="PEVDYPTIQVQTFYPGASPDVMTSSITAPLERQLGEMPGLNQMT
                     STSSAGASVITLQFSLDLTLDIAEQEVQAAINAAGNLLPSDLPAPPIYAKVNPADAPI
                     LTLAVTSKSMQLTQVEDLVDTRLAQKLSQLQGVGLVSIGGGQRPAVRIQANPRALAAY
                     GLNIDDLRTTISNANVNTPKGNFDGPSRAYTINANDQLKKPEEYRDLVVAYRNGAPVR
                     LSDVATVAESAENTKLGAWMNETPAVIVNIQRQPGANVIAVVDSIKALLPQLEASLPA
                     ALQVHVLTDRTNTIRASVRDVELELALAVGLVVLVIFLFLRDLRATLIPSLSVPLSLV
                     GTLAVMYLAGFSLDNLSLMALTIATGFVVDDAIVMIENISRYIERGVAPLQAALRGAE
                     QIGFTIVSLTISLIAVLIPLLFMGDVVGRLFREFAITLAVTILISAAVSLTLVPMLCA
                     KLLRHRRPTEMSAVARRSQAMFDGLIAGYGRMLSRVLDHQGLTLLVAGATLALTAYLY
                     VVIPKGFFPVQDTGLIQGISEAPQSVSYVAMAERQQKLAAAILKDPDVVSLSSFIGVD
                     GTNTTLNSGRFLINLKPRDERQASASEIIRRLQHEIAAIPGVSLYMQPAQDLTIDATV
                     SRTQYQFVLEDANPAEFTAWVPKLIDRLKQLPEIADVASNLQDQGLAAYIEIDRDTAA
                     RFGITPATVDNALYDAFGQRIVSTIFTQSNQYRVILETEPTQATLSGIHLPSSAGGQV
                     PLLSIARVIEQQAPLQ"
BASE COUNT          363 a          687 c          843 g          376 t
ORIGIN      
        1 tctgcagcgg cgcctgctgc tcgatcacgc gcgcgatcga cagcagcggc acctggccgc
       61 cggccgacga cggcaggtgg atgccggaga gcgtggcctg ggtcggctcg gtctcgagga
      121 tgacgcggta ctggttcgac tgcgtgaaga tggtcgagac gatgcgctgg ccgaaggcgt
      181 cgtagagcgc gttgtcgacc gttgccggag tgatgccgaa gcgcgccgcc gtgtcgcggt
      241 cgatctcgat ataggcggcg aggccctggt cctggaggtt gctcgccacg tcggcgatct
      301 cggggagctg cttcagccgg tctatcagct tcgggaccca ggcggtgaac tctgccgggt
      361 tggcgtcctc gaggacgaac tggtactgcg tgcggctcac cgtcgcgtcg atcgtcagat
      421 cctgcgccgg ctgcatgtag agcgagacgc cgggaatcgc cgcgatctcg tgctgcaagc
      481 gccggattat ctcgctcgcg cttgcctgcc gctcgtcacg cggcttcaaa ttgatgagga
      541 agcggccgct gttcagcgtc gtgttggtgc cgtcgacgcc gatgaaggag gagaggctga
      601 cgacgtccgg atccttgagg atcgccgcgg cgagcttctg ctggcgctcg gccatcgcga
      661 cgtaggagac gctctgcggc gcctcggaga tgccctggat gagcccggta tcctggaccg
      721 gaaagaagcc cttggggatg acgacgtaga gataggcggt gagggccagc gtcgcaccgg
      781 cgacgagcag cgtcagcccc tgatggtcga gaacgcggct cagcatccgg ccatagccgg
      841 cgatgagccc gtcgaacatt gcctggctgc gccgcgccac cgccgacatc tctgtagggc
      901 ggcggtggcg cagcagcttg gcgcagagca tcggcacgag ggtcagcgac accgcggccg
      961 agatgaggat ggtgacggcg agggtgatcg cgaactcgcg gaagaggcgc ccgaccacgt
     1021 cgcccatgaa gagcagcggg atcagcacgg cgatcagcga gatcgtcagc gacacgatcg
     1081 tgaagccgat ctgctcggcg ccgcgcagcg ccgcctgcaa gggcgcgacg ccccgctcga
     1141 tgtagcgcga gatgttctcg atcatgacga tggcgtcgtc gacgacgaag ccggtggcga
     1201 tcgtcagcgc catcagcgac agattgtcca ggctgaagcc ggcgaggtac atgaccgcca
     1261 gcgtgcccac aagcgaaagc ggcaccgaga ggctggggat cagcgtcgcg cgcagatcgc
     1321 gcaggaagag gaagatgacg aggacgacga gcccgaccgc gagggcgagc tcgagctcga
     1381 cgtcgcgtac cgaagcgcgg atggtgttcg tgcggtcggt cagcacatgc acctggagcg
     1441 ccgccggcag cgacgcctcg agctgcggca gcagcgcctt gatgctgtcg accaccgcga
     1501 tgacgttggc gccaggctgg cgctggatgt tgacgatgac cgccggcgtc tcgttcatcc
     1561 aggcgccgag cttggtgttc tcggcgctct ccgcgacggt cgccacgtcg gagaggcgga
     1621 ctggcgcgcc gttccggtag gcgacgacga ggtcgcggta ctcctccggc ttcttcagtt
     1681 ggtcgttggc gttgatcgtg taggcgcgcg acgggccgtc gaaattgccc ttgggcgtgt
     1741 tgacgttggc gttgctgatc gtcgtcctga ggtcgtcgat gttgaggccg taggcggcga
     1801 gcgcccgcgg attggcctgg atgcgcaccg ccggccgctg accgccgccg atgctgacga
     1861 ggccgacgcc ctggagctgc gagagcttct gcgcgagccg tgtgtcgacc agatcctcga
     1921 cctgggtaag ctgcatgctc ttcgaggtga cggcgagcgt caggatgggc gcgtcggcgg
     1981 ggtttacctt ggcatagatc ggcggcgccg gcaggtccga gggcaggagg ttgccggccg
     2041 cgttgatcgc cgcctgcacc tcctgctcgg cgatgtcgag ggtaaggtcg aggctgaact
     2101 gcaacgtgat gaccgaggcg ccggccgaac tcgtcgaggt catctggttg agccccggca
     2161 tctcgccgag ctggcgctcc agcggcgcgg tgatcgacga ggtcatcaca tccgggctgg
     2221 cgccgggata gaaggtctgc acctggatgg tcgggtagtc gacctcggg
//
LOCUS       DASRWS010000500         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3649259, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000500 DASRWS010000000
VERSION     DASRWS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3649259"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFF72_09395"
     CDS             <1..437
                     /locus_tag="VFF72_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV93421.1"
                     /translation="PAVAQAAEANFRSRGVRVIPYPSDMRAFAPHAQLCAIVHRWGGF
                     YTRVAESVIDGTWTNTPVWGGIASGMVDIAAVDPTLPASLRAAVQARRQAIVDGTLKP
                     FAAPLIDNTGRLRLAHGALDDAQIKSMNWLVAGVVGSVPRSR"
     gene            complement(459..1319)
                     /locus_tag="VFF72_09400"
     CDS             complement(459..1319)
                     /locus_tag="VFF72_09400"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZV93422.1"
                     /translation="MRPHLPPLFVSHGSPMIALEPGEAGAFLARLGPALDATFGRPKA
                     IVSISAHTAARLPVVLAAPRHEAVYDFGGFDPKLFTLRYDAPGDPGLAAHVVGLLQAA
                     GIAARSVPEGGLDHGAWTALRYIYPQADIPVVPLAFVPGDSPERQFALGAALAPLAGE
                     GVLVLGSGSITHNLRRVFRDGGLHPDKSQPEIPESAAFRQWIAGRSGALDWSALFAYR
                     SQAPFGVEMHPTDEHLLPWFVAAGAGGRSDAPLRVHASATMGSLGMDAYAFGPHAAAL
                     GEALGVLDAA"
     gene            1485..>2364
                     /locus_tag="VFF72_09405"
     CDS             1485..>2364
                     /locus_tag="VFF72_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93423.1"
                     /translation="MLRDLRAGELRLLVVAVTLAVAALTAVGFFADRINGGLARDARQ
                     LLGGDAIVASDQPVPPAFADKARALGLVTATTANFPSMGRAPDAKGGATRLVSVKAVS
                     DAYPLRGRLRIRDAAGADAATRDVAHGPAPGTAWVDAAVLDALGLKVGDTLLLGDASL
                     KTAEVIVIEPDRGTGFASLAPRVMLNVADLPSTGLVQPASRITYRLAVAANQVADDRV
                     NSFVHWAQTEVKDRHLRGMRVESIATGRPEMRQTLDRAGKFLNLVALLAALLAAVAVG
                     IAARDFASRHLDDCAML"
BASE COUNT          340 a          841 c          862 g          321 t
ORIGIN      
        1 cgccggcggt ggcgcaggcg gccgaggcca actttcgcag ccgcggcgtt cgcgtcatcc
       61 cgtaccccag cgacatgcgt gccttcgcgc cgcacgcgca gctgtgcgcc atcgtccatc
      121 gctggggcgg cttctacacg cgcgtcgccg agagcgtcat cgacggcacg tggacgaaca
      181 cgccggtctg gggcggcatc gccagcggca tggtcgacat cgctgcggtc gacccgacac
      241 tgcccgcctc gctgcgcgcg gcggtgcagg cgcgccggca ggcgatcgtc gacggcacgc
      301 tgaagccgtt cgcggcgcca ttgatcgaca acaccggtcg cctgcgactc gcccacggag
      361 cgctcgacga cgcgcagatc aagtcgatga actggctggt cgccggcgtc gtcggcagcg
      421 tgccgcgatc gcgctgaagc ggaattcgcc gacgcgcgtc aggcggcgtc gagcacgccg
      481 agggcttctc cgagcgccgc tgcgtgcggg ccgaaggcgt acgcgtccat gccgaggctg
      541 cccatcgtcg cgctggcatg cacgcgcagc ggcgcatcgc ttcggccacc ggcgccggcg
      601 gcgacgaacc agggcagcag gtgctcgtcg gtcggatgca tctcgacgcc gaacggagcc
      661 tggctgcgat acgcgaacag cgcgctccag tcgagggccc cggagcgtcc ggcgatccat
      721 tgacggaacg ccgcgctttc ggggatctcg ggctggctct tgtcagggtg caggccgccg
      781 tcgcgaaaga cgcgcctcag gttgtgcgtg atgctgccgc tgcccaggac gaggacgcct
      841 tcgccggcga gcggagccag ggcggcaccg agcgcgaact ggcgctcggg cgaatcgccc
      901 ggcacgaagg cgagcggcac gacgggaatg tcggcttgcg gatagatgta gcgaagcgcc
      961 gtccaggcgc catggtcgag gccgccttcg ggcaccgacc gggccgcgat tccggcggcc
     1021 tgaagcaagc cgacgacgtg cgcggcaagg cccggatcgc ccggcgcgtc gtagcgcagc
     1081 gtgaagagct tcggatcgaa gccgccgaag tcgtagaccg cttcgtggcg gggcgcggcg
     1141 agcacgaccg gaaggcgcgc cgccgtgtgt gccgagatgc tgacgatcgc cttcggccgg
     1201 ccgaaggtcg catcgagggc cgggccgagc cgggcgagaa aggcgccggc ctcgccgggc
     1261 tcgagggcga tcatcggcga gccgtgcgaa acgaacagcg ggggcaggtg agggcgcatg
     1321 ggaagattgg atcacgcgcc cgcccggcga cgattcaacc ctgctgcacg cagcgttcgc
     1381 cactcgtgta agttcgacgc cgcatgagcc cgccgccgac aacctccgcc gccgacgcgg
     1441 ctgtgccgcg accgcccggt ccctggcggc tcggcacccg gcagatgctg cgcgacctgc
     1501 gcgcgggcga gttgcgattg ctggtcgtcg ccgtgacgct ggcggtcgcc gccctgaccg
     1561 ccgtcgggtt cttcgccgat cgcatcaacg gcggtcttgc ccgcgatgcg cggcagcttc
     1621 tcggcggcga cgcgatcgtc gccagcgacc agccggtgcc gccggcgttc gccgacaagg
     1681 cccgcgcgct cggcctggtc accgcgacga cggcgaactt cccgagcatg ggccgggctc
     1741 ccgacgcgaa gggcggcgcg acgcggctgg tgagcgtcaa ggccgtgagc gatgcctatc
     1801 cactgcgcgg acgactgcga atccgcgatg cggccggtgc cgatgcggcg acgcgagacg
     1861 tggcgcatgg gccggcgccg ggcacggcgt gggtcgatgc ggcggtgctc gatgccctcg
     1921 gcctgaaggt cggcgacacc ttgttgctgg gcgatgccag cctgaagact gccgaggtca
     1981 tcgtcatcga acccgatcgc ggcaccggct tcgccagcct ggcgccgcgc gtcatgctca
     2041 acgtcgccga cctgccgtcg accggcctcg tccagccggc gagccgcatc acgtatcgcc
     2101 tggccgttgc cgctaaccag gtcgccgacg acagggtgaa cagcttcgtc cactgggcgc
     2161 agaccgaggt gaaggaccgt catctgcgcg gcatgcgcgt cgagtcgatc gccaccggcc
     2221 ggcccgagat gcgccagacg ctcgatcgcg ccgggaaatt cctcaacctc gtcgccctgc
     2281 tcgcggcgct cctcgccgcc gtggccgtcg gcatcgccgc gcgcgatttc gccagccgcc
     2341 acctcgacga ttgcgcgatg cttc
//
LOCUS       DASRWS010000501         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1070999, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000501 DASRWS010000000
VERSION     DASRWS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070999"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFF72_09410"
     CDS             <1..311
                     /locus_tag="VFF72_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93424.1"
                     /translation="AQACREDAACRSRTDDDVVEFVRHGRSLSVRYATGREPSNAAHP
                     VRPADERNARFDLNAILGSGSRGSGRRRVHAPGEGTTLAALEGLGAPREEAPRLEQRT
                     "
     gene            369..800
                     /locus_tag="VFF72_09415"
     CDS             369..800
                     /locus_tag="VFF72_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModE family transcriptional regulator"
                     /protein_id="HZV93425.1"
                     /translation="MDATAMIARKTAAAKIGAKTTTTPSAAKPATPVARFRMRITSGD
                     TISVGPGKIALLEAIRAERSITAAAQSIGMSYRRAWMLVDELNAALKKSAVRSIKGGD
                     HGGGSELTEEGAMLVAVYRRIEEQAAKACAAEIKALLKLVR"
     gene            complement(877..1116)
                     /locus_tag="VFF72_09420"
     CDS             complement(877..1116)
                     /locus_tag="VFF72_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZV93426.1"
                     /translation="MIVVADLDGARLSARNQLAGTIVRMQPGAVNAEVVIDVGGGATI
                     VALVTQGSASSLALAPGVAASAVFKASSVIVGVPR"
     gene            complement(1114..1998)
                     /locus_tag="VFF72_09425"
                     /pseudo
     CDS             complement(1114..1998)
                     /locus_tag="VFF72_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2009..2683)
                     /gene="modB"
                     /locus_tag="VFF72_09430"
     CDS             complement(2009..2683)
                     /gene="modB"
                     /locus_tag="VFF72_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZV93427.1"
                     /translation="MDAAWIALGLSLKVALWATGLDLVLGVAIGYALARKRFPGRELV
                     DSILLLPMVLPPTVLGYYLLVVIGRRGWLGGWLSREFGIDLVFTWQGAVLAATLVAFP
                     FVCKSARAAFETVDAQYEEAAKVLGLGAAAIFLRVTLPLAWRGILAGVLLAFARALGE
                     FGATLMVAGSIPGKTQTLSIAVYEAVQAGQDDLANLLVLLTSTACVVVLLVAGRLAPA
                     RIPGRP"
     gene            complement(2707..>3052)
                     /gene="modA"
                     /locus_tag="VFF72_09435"
     CDS             complement(2707..>3052)
                     /gene="modA"
                     /locus_tag="VFF72_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV93428.1"
                     /translation="PASVPVGRYTKAVLEKAQLWPAIEARMIGAQSVRQALDYVARGE
                     VDAGFVYGTDAAIMRGKVKVAFVVPTETPVLYPIAATASAPNPAGAREFVAFVASPAA
                     QAILARFGFGKP"
BASE COUNT          515 a         1043 c         1103 g          391 t
ORIGIN      
        1 tcgctcaggc gtgccgcgag gacgcagcct gccgatcccg caccgacgac gatgtagtcg
       61 aattcgtccg ccatggccga tcgctctcag taaggtacgc aacaggccgg gagccgtcga
      121 acgcggcgca tccggtacgg cctgccgatg aacggaacgc ccgtttcgat ctgaacgcga
      181 tcttaggctc aggcagccgc ggttcgggcc gccgtcgcgt gcacgcgccg ggtgagggga
      241 cgaccctcgc cgcgctcgaa gggctgggcg cgccgcgcga ggaggcgcca aggctcgagc
      301 agcgcacgtg agccggcacc gcgcttcgcc gatggtgtaa ggtgccacgg cacaagtccg
      361 cgaaatcgat ggatgccacc gccatgattg ccaggaagac tgccgccgcg aagatcggcg
      421 cgaagacgac gacaacgcct tcggccgcga agccggcgac gccggtcgcg cgcttccgga
      481 tgcgcatcac gtcgggcgac acgatctcgg tcgggccggg caagatcgcc ctgctcgagg
      541 cgatccgcgc ggagcggtcc attaccgccg cggcgcagtc gatcggcatg tcgtaccggc
      601 gcgcctggat gctggtcgac gagctcaatg cggcgctgaa gaagtcggcc gtgcgctcga
      661 tcaagggggg cgaccacggc ggcggcagcg agctcacgga agaaggcgcc atgctcgtgg
      721 ccgtctaccg ccggatcgag gagcaggcag ccaaggcctg cgccgccgag atcaaggcgt
      781 tgctgaagct ggtgcgctga ggcagccggg tcgcgccggt cgattcggcg cgaagacgca
      841 ctgccgggct gccgtccctg gcgcacggcc tggcgctcag cgaggaacgc cgacgatcac
      901 gctcgatgcc ttgaagaccg cgctggccgc gacgcccggc gccagcgcaa gcgaagacgc
      961 gctgccctgg gtgacgagcg cgacgatcgt cgcgccgccg ccgacatcga tcaccacctc
     1021 ggcgttgacg gcaccgggct gcatccgcac gatcgtcccg gcaagctggt tgcgggccga
     1081 cagacgtgca ccgtcgagat cggcgacgac gatcaccgac gacgccttga cgagggcgaa
     1141 ggcatcgatg ccgaccgcga gtccgagctt cgtgacgctt tcgtgggtga cgatggcgac
     1201 gatgctctgg ccgccgggca aggcgatctc gacctcgtcg tcgacggcgc ctggcaggat
     1261 gcggctcacc cttccggtga gctggttgcg ggcactcgat ttcatggccg attcctcctg
     1321 gacgggaacc tgcgacgccg tggcgcgacc ggcatcgatg tagacgatct cgtcggcaaa
     1381 ggcgtcgacg tcggccgaat cgtgggtgat gagcacgatc ggcagcgcaa aacgggcctg
     1441 cagctcatgc agctcggcgc gcagcttgcc gcgcagcggc aggtccatcg cggcgaacgg
     1501 ctcgtcgaga aggagcgcgc gggggctggc ggcgagcgcg cgggccagcg cgacgcgctg
     1561 ccgttgaccg cccgagagct ggtcgggaaa gcgcgcctgc agccgacgca gctcgagcgc
     1621 gtcgagccat cgatcggtcg tctcgtcggc gcggcctcga cgcgggttgc gcaggccgct
     1681 gtcgaggccg aaggcgatgt tctggcgcac cgtcagatgc gggaagagcg cgtattcctg
     1741 gaacaggtag cccaggcgcc gcgagcgcgc cgccaggtcg actccggcgc cggcgtcgaa
     1801 catcgcctcg ccgtcgaagg cgattcggcc ggcatcgggg cggaccagtc cggcgatcgc
     1861 cttcagcgtc atcgtcttgc cggcgcccga gggaccgaag aggacgaggc ggcgcgcatc
     1921 gctggcaaac gacatgtcga gcgaaaaggc attgggtccg tcgccgacct gctttcgtat
     1981 cgccacttcc cacttcatgg cgacgacctc acggacggcc cgggatccgc gccggggcga
     2041 gccggccggc gaccaggagg acgacgacac aggcggtcga agtcagcagc accagcaggt
     2101 tggcgaggtc gtcctgaccc gcctgcaccg cctcgtagac ggcgatcgag agcgtctgcg
     2161 tcttgcccgg aatgctgccg gcgaccatca gcgtcgcgcc gaattcgccg agggcacgcg
     2221 cgaaggcgag cagcacgccg gcgaggatgc cacgccaggc cagcggcagc gtgacgcgaa
     2281 ggaagatcgc cgcggcaccg aggcccagga ccttggccgc ctcctcgtac tgggcgtcga
     2341 ccgtctcgaa cgcggcgcgc gccgacttgc acacgaacgg gaaggcgacg agggtcgccg
     2401 ccaggaccgc gccctgccag gtgaagacga ggtcgatgcc gaactcgcgc gacagccatc
     2461 cgccgagcca gccacgccgg ccgatgacga cgagcaggta gtagccgagt accgtcggcg
     2521 gcagcaccat cggcagcagg aggatcgagt cgacgagctc ccggccaggg aagcgctttc
     2581 gcgccagcgc gtagccgatt gcgacaccga ggacgaggtc caggccggtc gcccacagcg
     2641 ccaccttcag cgacagcccg agggcgatcc acgccgcgtc caccgtgccg ggcccgggtc
     2701 cgcggatcag ggtttgccga agccgaagcg ggccaggatg gcctgcgcgg cgggcgaagc
     2761 gacgaaggcg acgaactccc tcgcgccggc aggattcggc gccgaggcgg tcgcggcgat
     2821 cggatagagc accggcgttt cggtgggcac gacgaaggcc accttcacct tgccgcgcat
     2881 gatggcggcg tcggtgccgt agacaaagcc ggcatcgacc tcgccgcgcg ccacgtagtc
     2941 gagcgcctgt cgcaccgact gtgcgccgat catcctggcc tcgatcgccg gccaaagctg
     3001 cgccttctcg agcacggcct tcgtgtagcg gccgaccggc acgctggccg gc
//
LOCUS       DASRWS010000502         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1657828, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000502 DASRWS010000000
VERSION     DASRWS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657828"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1160
                     /locus_tag="VFF72_09440"
     CDS             174..1160
                     /locus_tag="VFF72_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93429.1"
                     /translation="MAETDQQRVQKVLDTQVKPLAARGAGEMHAALGKLRSSIHKVEH
                     GIKQRVDAEMLSTYRRELTIDMRGVVDAAVTLRKALNALADATKDDEDFAADEAGIEK
                     MKSGILAVLDGSKVDLRKAKELEDRAAEQIDKVGSSEVEAKKMWAGVVAVWEKNDHVV
                     AKALKDLRQWKKDAGEAVAARDASRLKQLQANPPAIPPDEKLLKGTLNNDMMVRFTHD
                     FHLDGFSRAFRDEVLADAARMKQADKDGQKSGQEWKDILATVAKLSIVPRDAKKAAAV
                     LGLPAGASAKLQPVLELDAGAMPKALESLGKQFKVDLDGKDALAKLRKNGVL"
     gene            1234..2832
                     /gene="garD"
                     /locus_tag="VFF72_09445"
     CDS             1234..2832
                     /gene="garD"
                     /locus_tag="VFF72_09445"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HZV93430.1"
                     /translation="MSEAPLLLPPHVIRMDARDNVAIVANGGGLEVGTLFLAGPAAGL
                     TLRDRVPQGHKMALVDIAEGEPVLRYGVTIGRALKPIARGSWVHERLLRMPSARSLEG
                     LPVATIRPADAAPLEGFTFEGFRNADGTVGTRNILAITTTVQCVAGVVDAAVARIRNE
                     LLPRYPNVDDVVGLEHGYGCGVAIDAVDAPIPIRTLRNISRNPNFGGEVMVVSLGCEK
                     LQPERLLPPGTFAIRDERSDAPGGEFDLVRLQDERHVGFASMLDSILATARVHLERLN
                     ARRRETVPASALVVGVQCGGSDAFSGATANPAVGVCTDLLVRAGATVMFSETTEVRDG
                     IDQLTARAANAEVAAAMIREMAWYDAYLGRGGADRSANTTPGNKAGGLSNIVEKAMGS
                     IVKSGTSPIGGVLSPGESLRDKGIAGGLVYAATPASDFICGTLQMAAGMNVHIFTTGR
                     GTPYGLAQVPVVKVATNSELARRWHDLMDLNAGRIADGTATIEQCGRELFELVLEVAG
                     GRRTWAERHGLANDLVLFNPAPVT"
BASE COUNT          526 a         1056 c         1094 g          432 t
ORIGIN      
        1 ggcgggacgg attcatggtc aggctccagt gtggaggatg caacgattgc gggaacgcgc
       61 gtcgccgtca atcggggacg ccatccgacc gcgtgggggg tcgattgcgt cgcggccggc
      121 aaagtcccac actgtcgccc cggctgcccg ggcgacgcac gaggagcgaa gcgatggccg
      181 aaaccgatca gcagcgcgtg cagaaggtgc tcgacaccca ggtcaaaccg ctcgctgcgc
      241 gcggcgccgg cgaaatgcat gccgcgctcg gcaagctgcg cagctcgata cacaaggtcg
      301 agcacggaat caagcagcgc gtcgacgccg aaatgctttc cacctatcga cgcgagctga
      361 cgatcgacat gcgtggcgtt gtcgacgcgg cagtcaccct gcgcaaggcc ctgaatgcgc
      421 tcgccgacgc gacgaaggac gatgaggatt tcgccgccga cgaggctggc atcgagaaga
      481 tgaagagcgg catcctcgcc gtcctcgacg gctcgaaggt cgacctgcgc aaggccaagg
      541 agctcgagga ccgggctgcc gagcagatcg acaaggtggg cagcagcgag gtcgaggcga
      601 agaagatgtg ggccggcgtc gtcgccgtct gggagaagaa cgaccacgtc gtcgccaagg
      661 cgctgaagga tctgcggcaa tggaagaagg acgccggcga ggccgtcgct gcgcgcgacg
      721 cttctcgcct caagcagttg caggccaatc cgcctgccat cccgcccgac gagaagctcc
      781 tcaagggcac cctcaacaac gacatgatgg ttcgcttcac ccacgacttc catctcgacg
      841 gattcagtcg cgcctttcgc gacgaggttc ttgccgacgc cgccaggatg aagcaggccg
      901 acaaggacgg gcagaagtcg ggccaggagt ggaaagacat cctggcgacg gtggccaagc
      961 tttcgatcgt gccgcgcgac gcgaagaagg ccgccgccgt cctcggcctg cccgcgggcg
     1021 cgtcggcgaa gctgcagccc gtcctcgagc tcgatgccgg cgcgatgccg aaggcgctcg
     1081 agtccctggg caagcagttc aaggtcgatc tcgacggcaa agacgcgctg gccaagctgc
     1141 gcaagaacgg cgtgctctga cgcgcgagcg cgccggcggg ccttcgatgc cacgccccgg
     1201 tcgcggcatc gatgcgggtc gcaaaatggc gccatgagcg aagcgcccct cctcctgccg
     1261 ccgcacgtga tccgcatgga cgcgcgcgac aacgtcgcca tcgtggccaa tggcggcggc
     1321 ctcgaagtcg gcaccctctt tcttgccggc cccgcggctg gcctgactct gcgcgaccgt
     1381 gtgccgcagg ggcacaagat ggcgctggtc gacatcgccg agggcgagcc cgtgcttcgc
     1441 tacggcgtga ccatcggacg cgccttgaaa ccgatcgccc gcggcagctg ggtccatgag
     1501 cggctgctgc gcatgccgtc ggcgcgctcg ctcgagggcc tgccggtcgc caccatcagg
     1561 ccggctgatg cggcgccgct cgaaggcttc acgttcgaag gattccgcaa tgccgacggc
     1621 acggtcggga cgcgcaacat cctggcgatc acgacgacgg tgcagtgcgt cgccggcgtc
     1681 gtcgacgccg ccgtcgcgcg catcaggaac gagcttcttc cgcgctaccc gaacgtcgac
     1741 gacgtcgtcg gcctcgagca cggctacggc tgcggcgtcg ccatcgacgc cgtcgatgcg
     1801 ccgatcccga tccgcacact gagaaacatc agccgcaatc cgaacttcgg cggcgaggtg
     1861 atggtggtga gcctgggctg cgagaagctg cagcccgagc ggctgctgcc gccgggcacg
     1921 ttcgcgatcc gcgacgaacg cagcgatgcg ccgggcggcg agttcgacct ggtgcgcctg
     1981 caggacgagc gtcacgtcgg cttcgcctcg atgctcgatt cgatcctcgc caccgcgcgc
     2041 gtccatctcg agcggctgaa cgcacgacgc cgcgagacgg tgccggcgtc ggcgctcgtc
     2101 gtcggcgtgc agtgcggcgg cagcgacgct ttctcgggcg ccaccgccaa cccggcggtc
     2161 ggcgtctgca ccgacttgct ggtgcgcgcc ggcgcgaccg tgatgttctc ggagacgacg
     2221 gaagtgcgcg atggcatcga ccagctcacc gcgcgtgccg cgaacgccga agtcgccgcg
     2281 gcgatgatcc gcgagatggc ctggtacgac gcctatctcg gccgcggcgg tgccgaccgc
     2341 agtgccaaca cgacgccggg caacaaggcg ggcggcctgt cgaacatcgt cgagaaggcg
     2401 atgggctcga tcgtcaagtc gggcacgagc ccgatcggcg gcgtgctctc gcccggcgaa
     2461 agcctgcgcg acaagggcat cgccggcggc ctcgtctatg cggcgacgcc ggccagcgac
     2521 ttcatctgcg gcacgctgca gatggccgcc ggcatgaacg tgcacatctt caccaccggc
     2581 cgcggcacgc cctacgggct ggcccaggtg ccggtcgtca aggtggcgac gaacagcgag
     2641 ctggcccggc gctggcacga cctgatggac ctcaacgccg ggcgcatcgc cgacggcacg
     2701 gcgacgatcg agcaatgcgg tcgtgagctg ttcgagctcg ttctcgaggt ggccggtggc
     2761 cggcgaacct gggccgagcg gcacggcctg gccaacgacc tcgtgctgtt caacccggcg
     2821 ccggtgacct gaggacgcgc acggaccggc atgggttgcc ggcctcggcg gtccggcttg
     2881 ccggtcgatt cgcggaccac gagcgacgcg cgggcctcgt ggtgcagggg tcgccttccc
     2941 gcgctgcgac aatgcttcga accgggccag gtcgatggag cgagcgtgac ggacgaagac
     3001 atcggcgcgc agcgtgcagc gctcgcggcg catttcaagg cgaggatccc ggccttgctc
     3061 gaggcctggc gtgcggccgt cgacgcggat ccgaaactca cgaccggc
//
LOCUS       DASRWS010000503         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_212970, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000503 DASRWS010000000
VERSION     DASRWS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212970"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1289
                     /locus_tag="VFF72_09450"
     CDS             48..1289
                     /locus_tag="VFF72_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HZV93431.1"
                     /translation="MLSADSTTPLEASHVAFDDEEGPAVGLTDLLTWLGQGKKTIAAV
                     TVAAALVSLAVALLLPPFFTARTTFLAPGSQQQSGSAAALAALGSLGALGGGLVPKSP
                     DELYVALLKSDSVDRALDRRFDLQKRYRVDSYETLKKTLPNFIRVSSDKKSGVIAVEV
                     DDEDPQFAARLANAHEGEITQLLGRLAVSEAQLRRVFFAKQLEETKESLVRAELNLQK
                     VQEKSGVIVLDKQAEALINRAALVRAQISEREVQLRVLRTSATEQNPEVIRLNSELAG
                     LRAELARMESSKGGAPGSAIDLPVGKIPEAALEYIRARREMKLQETLLESMVRQYEIA
                     KLDEAKEGPTLQQVDVAVAPDHKSKPSRALIVIAGTLLGFLLASAWVVIRRYSRLVRE
                     ADPSREAAWAEMGRAWGFRRA"
     gene            complement(1300..2073)
                     /locus_tag="VFF72_09455"
     CDS             complement(1300..2073)
                     /locus_tag="VFF72_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV93432.1"
                     /translation="MSVGASARLAFDHLVVAARSIGEGVAWCEAELGVVPAAGGKHPL
                     MGTHNRLVNVGSVRYPRSFLEIVAVDPDAPAPGRPRWFDLDGEATRAALASGPKLVQW
                     VARTDAIDASLVALRAAGLALADPNAAERLTARGLLRWRIALMADGSRPAGGALPLLI
                     EWGGEHPCDSLPTSAVVLEQVELAGVSASLATMLGVDAAPTMSSIDGSGISEAEAEAE
                     AEDAPSLRGTAGTTTAAGIIVVLRTPIGLRRLSSPPSTG"
BASE COUNT          358 a          804 c          748 g          312 t
ORIGIN      
        1 gttcgggctc ggcatcgccg cgatccattc gctctgatcg gccggccatg ctcagcgcag
       61 actcgaccac gccgctcgaa gcctctcatg tcgccttcga cgacgaggaa gggcccgccg
      121 tcggcctgac cgaccttctc acctggctcg gccagggaaa gaagacgatc gccgccgtca
      181 ccgtcgcggc agcgctcgtg tcgctcgccg tcgccttgct gctgccacct ttcttcaccg
      241 cgcgaaccac ttttctcgct ccgggctcgc agcagcaaag cggctctgcg gccgccctcg
      301 ccgcgctcgg ctcgcttggc gctctcggcg gcggcctggt gccgaagtct cccgacgagc
      361 tttacgtggc gctgctgaag agcgacagcg tcgaccgcgc cctcgatcga cgcttcgacc
      421 tgcaaaagcg ctaccgggtc gacagctacg agacgctgaa aaagactctg cccaacttca
      481 tccgcgtctc ttcggacaag aagagcggcg tcatcgccgt cgaggtcgac gacgaagatc
      541 cgcagttcgc cgcgaggctc gccaatgcgc acgaaggtga gatcacgcaa ctgctcggcc
      601 ggctcgccgt ctccgaggcg cagctgcgcc gtgtcttctt cgccaagcag ctcgaagaga
      661 ccaaggaaag cctcgtccgc gccgagctca acctgcagaa ggtgcaggaa aaatccggcg
      721 tcatcgtcct cgacaagcag gccgaggcct tgatcaaccg cgccgcgctg gtgcgtgcgc
      781 agatctccga gcgcgaggtc cagctcaggg tcctgcgcac ctcggcgacg gagcagaacc
      841 ccgaagtgat ccgcctcaac tccgagctcg cgggactgcg cgccgagctg gcccggatgg
      901 agtcgtcgaa aggcggcgcc cccggcagcg ccatcgacct gccggtcggc aagatccccg
      961 aggcggccct cgaatacatt cgcgcccgtc gcgagatgaa gctgcaggag accttgcttg
     1021 aaagcatggt ccgccagtac gagatcgcca agctcgacga agccaaggaa ggcccgacgc
     1081 tgcagcaggt cgatgtcgcg gtcgcgccgg accacaagtc gaagccgtcg cgggcgctca
     1141 tcgtcatcgc cggcacgctg ctcggcttcc tgctggcctc ggcgtgggtg gtgatccgcc
     1201 gctattcgcg cctcgtcagg gaggccgatc cttcacgcga ggcggcctgg gccgagatgg
     1261 gccgcgcctg gggattccgc cgggcctgac ggccagcgct cagccggtcg acggcggcga
     1321 cgaaaggcga cgcaggccga tcggcgtgcg caagacgacg atgatgccgg cggccgtcgt
     1381 cgtgccggcg gtcccgcgca gcgacggcgc atcttcggct tcggcttcgg cttcggcctc
     1441 cgaaatcccg cttccgtcga tcgacgacat ggtcggcgcc gcatcgacgc cgagcatcgt
     1501 cgccagcgat gccgaaacgc cggcgagttc gacctgctcg agaacgacgg cgctggtcgg
     1561 cagggagtcg cacggatgct cgccgcccca ctcgatgagc aacggcaggg cgccgccggc
     1621 cgggcggctg ccgtcggcca tcaaggcgat gcgccagcgc agcaggccgc gggccgtgag
     1681 gcgttcggca gcgttcggat cggccagcgc cagacccgcg gcgcgcagcg cgacgagact
     1741 cgcatcgatc gcatcggtgc gggccaccca ttgcacgagc ttgggcccgc tggcgagggc
     1801 cgcgcgcgtg gcctcgccgt cgaggtcgaa ccagcgcggc cggccgggcg caggcgcgtc
     1861 cgggtccacc gcgacgatct cgagaaaact gcgcggatac cggaccgatc ccacgttgac
     1921 caagcggttg tgcgtgccca tcaggggatg cttgccacca gcggccggca cgacgccgag
     1981 ctcggcctcg caccacgcga cgccctcgcc gatcgagcgg gctgcgacaa cgaggtgatc
     2041 gaacgcgagc cgcgccgacg cgccgacgct cacggcagct cgacctcgcc gcgaatgcag
     2101 gtgacgccgt gtccgccgac ccagacgact ccgtcctggc aggcgatgtg gacgcggccg
     2161 cgccggccga gccgggtgcc ttgcgtggcg acgtaccgcg ccggcgccac acccgtctcg
     2221 at
//
LOCUS       DASRWS010000504         3977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1071948, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000504 DASRWS010000000
VERSION     DASRWS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071948"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /locus_tag="VFF72_09460"
     CDS             <1..1937
                     /locus_tag="VFF72_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZV93433.1"
                     /translation="AVPDPARWSEVFREATWEEALDLAGGTLRRLRDGNGRMSLAGFG
                     SAKGSNEEAYLFQKLVRTGFRNNNVDHCTRLCHASSVAALLEGVGSGAVSNPVNDVAH
                     AELILIIGSNPTSNHPVAATWMKNAAQRGTRIVLADPRRTEISRHAWRTLQFNADTDV
                     AMLNALIHTVIEEGLVDQSFVSERVDNYEALKENVRGYSPEAMAPICGIPAATLREVA
                     RAFATAKSAMILWGMGISQHVHGTDNARCLIALASVTGQIGKPGSGLHPLRGQNNVQG
                     ASDAGLIPMMFPDYRRVDNPAAHEWFERFWETPLDSVAGYTVVEIMHKVLAPDSDPHK
                     IRGMYIMGENPAMSDPDLNHARHALASLEMLVVQDIFMTETAWLADVVLPASAWPEKS
                     GTVSNTDRMVQLGRRALDMPGEARLDLWIIQQIANRLGLDWNYPGEDAGVAAVYEEMR
                     QAMHGAIAGITWERLQREDSVTYPCLSAEDPGRPIVFDDRFPTADGRVKLVPADIIPA
                     NERPDADFPFVLITGRQLEHWHTGSMTRRASMLDAIEPMPMASMNGADLAEIAVEPGD
                     VVTLLSRRGDVALHVRRDDGTPRGAVFVPFAYYEAAANLMTNSALDPFGKIPEFKYCA
                     IRVVAGGTVRPSPGYGTGAC"
     gene            1948..2577
                     /locus_tag="VFF72_09465"
     CDS             1948..2577
                     /locus_tag="VFF72_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93434.1"
                     /translation="MSSSAALADRVRARLDERIRGGVRDVTLLLHSDVAGSFVVESLQ
                     RQAKGGAPLNGLPARVATLAVTDVGAVGIDLWLAAFAWGAAQVWTLVTHEDEMPSREA
                     IARRMRVAEAILASVGLPGEHLRIIGGGEVSRLASCVAAKQGRRASPDERVDSGGDET
                     MPAALQRLDRALQRPPAAAIARRATFTGAGSGPGLLDLAFAHLLEVAPS"
     gene            2574..3464
                     /locus_tag="VFF72_09470"
     CDS             2574..3464
                     /locus_tag="VFF72_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZV93435.1"
                     /translation="MSDPNDTDRPARLRLTAARAPLTESIAVRDEFGAERVVSIPAER
                     ALTVFVDRRELVTLMTLGAAPELLVLGYLRNQRLVERVDDIESITVDWDVGAAAVKTR
                     AGIERFEEKTARRVVTTGCGQGTVFGDLMGELDTIHLLPADDPRGRLGQAALYGLLDA
                     VRRQESTYKSAGSVHGCALFLQSEMLMFVEDVGRHNAIDTIAGWMWLNGIDGADKIFY
                     TTGRLTSEMVIKSAQMGVPIVVSRSGVTQMGHAMAVRLGLALFGRATNRHFLCYCGFE
                     RFDAQPEPPRAAAAVTRPAD"
     gene            complement(3543..>3977)
                     /gene="mobA"
                     /locus_tag="VFF72_09475"
     CDS             complement(3543..>3977)
                     /gene="mobA"
                     /locus_tag="VFF72_09475"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:1902758 - bis(molybdopterin guanine
                     dinucleotide)molybdenum biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZV93436.1"
                     /translation="RHLETYAGMGSPVWPDATPDYPGPLAGFLAGLEHCTTPWLVTVP
                     CDSPLFPVDLVAQLVRGIERDDADVAMAATREEGALCLQPVFCLMRATVRDALSRFVA
                     SGRRKIDAWTATQRTATVEFDDAHAFVNANTLDELSKLQRPA"
BASE COUNT          606 a         1427 c         1376 g          568 t
ORIGIN      
        1 ccgcggtgcc cgatccggcg cgttggtcgg aggtgtttcg cgaagcgaca tgggaggagg
       61 cgctcgacct cgccggcggc accctgcgcc ggctgcgcga cggcaacggt cgcatgtcgc
      121 tggccggttt cggctcggcc aagggcagca acgaggaagc gtacctgttc cagaagctgg
      181 tccgcaccgg ctttcgcaac aacaacgtcg atcactgcac gcgcctgtgc catgcatcga
      241 gcgtcgcggc gctgctcgag ggcgtcggct cgggcgcggt gagcaacccg gtcaacgacg
      301 tcgcgcacgc cgagctgatc ctgatcatcg gctcgaaccc gacgtcgaac catccggtcg
      361 cggcgacgtg gatgaagaac gcggcgcagc gcggcacccg gatcgtcctc gccgacccgc
      421 gccgtaccga gatctcgcgc cacgcgtggc gaacgctgca gttcaacgcc gacaccgacg
      481 tcgcgatgct caacgcgctg atccacacgg tgatcgagga gggcctggtc gaccagtcgt
      541 tcgtcagcga gcgcgtcgac aactacgagg cgctcaagga aaacgtccgc ggctacagcc
      601 ccgaggcgat ggcgccgatc tgcggcatcc cggcagcgac gctgcgcgag gtcgcccgtg
      661 cgttcgccac cgcgaagagc gcgatgatcc tgtggggcat gggcatcagc cagcacgtgc
      721 acggcaccga caacgcgcgc tgcctgatcg cgctggcctc ggtcaccggc cagatcggca
      781 agcccggctc cggactgcac ccgctgcgcg gccagaacaa tgtccagggc gcgagcgacg
      841 ccggcctgat cccgatgatg tttcccgact accgccgggt cgacaacccg gccgcgcacg
      901 aatggttcga gcgcttctgg gagacgccgc tcgacagcgt cgccggctac acggtggtcg
      961 agatcatgca caaggtgctg gcgcccgaca gcgacccgca caagatccgc ggcatgtaca
     1021 tcatgggcga gaacccggcg atgagcgacc cggacctcaa ccacgcccgc catgcgctgg
     1081 catcgctcga gatgctggtc gtgcaggaca tcttcatgac cgagacggcg tggctcgccg
     1141 acgtcgtgct gcccgccagc gcgtggcccg aaaagagcgg caccgtcagc aacaccgacc
     1201 ggatggtcca gctcggccgg cgcgccctcg acatgcccgg cgaggcgcgg ctcgacctgt
     1261 ggatcatcca gcagatcgcc aaccgtctcg gcctcgactg gaactatccg ggcgaggacg
     1321 ccggcgtcgc cgcggtctac gaagagatgc gccaggcgat gcacggagcg atcgccggca
     1381 tcacgtggga gcggctgcag cgcgaggaca gcgtcaccta tccgtgcctc agcgccgaag
     1441 acccgggccg gccgatcgtc ttcgacgatc ggttcccgac cgccgacggc cgcgtcaagc
     1501 tcgtgccggc cgacatcatc ccggccaacg agcggcccga cgccgacttc ccgttcgtcc
     1561 tcatcaccgg ccggcagctc gagcactggc acaccggcag catgacgcgg cgcgccagca
     1621 tgctcgacgc catcgagccg atgccgatgg cgtcgatgaa tggcgccgac ctcgccgaga
     1681 tcgccgtcga gccgggcgat gtcgtcacct tgctctcgcg ccgcggcgat gtcgcgctgc
     1741 acgttcgccg cgacgacggc acgccgcgcg gcgctgtctt cgtgcccttc gcctactacg
     1801 aggcggccgc caacctgatg accaactcgg cgctcgatcc gttcggcaag atccccgagt
     1861 tcaagtactg cgcgatccgg gtcgtcgccg gcggcaccgt ccgtccgagc cctggctacg
     1921 gcactggcgc ctgctgaggg cgcatccttg tcctcctcgg cggcgctcgc tgaccgggta
     1981 cgtgcgcggc tcgacgagcg aatccgcggc ggcgttcgcg atgtcacgct gctcttgcac
     2041 agcgacgtgg cgggcagctt cgtcgtcgag tcgttgcagc gtcaagcgaa gggcggcgcg
     2101 ccgctgaacg gcctgcccgc acgtgtcgcg acgctggccg tgaccgacgt cggcgcggtc
     2161 ggcatcgacc tgtggctggc cgctttcgcg tggggcgcgg cccaggtctg gacgctggtg
     2221 acgcacgaag acgaaatgcc gtcacgcgag gcgatcgcgc gccggatgcg ggtcgccgag
     2281 gcgattctcg ccagcgtcgg cctgcccggc gagcacctgc gcatcatcgg cggcggcgag
     2341 gtgtcgcggc tggcgtcgtg cgtcgccgcg aagcagggtc gtcgagcctc gcccgacgag
     2401 cgcgtcgact ccggcggcga cgagacgatg ccggcggcgc tgcaacggct cgaccgggcg
     2461 ctgcaacggc cgccggcggc cgcgatcgcc cgccgcgcca ccttcactgg cgccggcagc
     2521 gggcccggct tgctcgacct tgccttcgcc catctgctcg aggtcgcgcc ttcgtgagcg
     2581 accccaacga caccgatcgg ccggcgaggc ttcgcctcac cgcggcgcgg gcgccgctca
     2641 ccgagagcat tgccgtgcgc gacgagttcg gcgccgagcg tgtcgtctcg atccccgccg
     2701 agcgcgcgtt gacggtgttc gtcgaccggc gcgagctggt gacgctgatg acgctcggcg
     2761 ccgcgcccga gctgctggtg ctcggctatc tgcgcaacca gcgcctcgtc gagcgcgtcg
     2821 acgacatcga gtcgatcacc gtcgactggg acgtcggcgc ggcggcggtg aagacgcggg
     2881 ccggcatcga gcgcttcgag gaaaagaccg ccaggcgcgt cgtcaccacc ggctgcggcc
     2941 agggcaccgt cttcggcgac ctgatgggcg agctcgacac gatccacctc ctgcccgccg
     3001 acgatccgcg cggccgcctc ggccaggccg cgctctatgg cctgctcgac gccgtgcgtc
     3061 gccaggagag cacctacaag tccgcgggct cggtgcacgg ctgcgcgctg ttcctgcaaa
     3121 gcgagatgct gatgttcgtc gaggatgtcg gccggcacaa cgcgatcgac accatcgcgg
     3181 gctggatgtg gctgaacggc atcgacggcg ccgacaagat cttctacacg acgggccgcc
     3241 tgaccagcga gatggtgatc aagtcggcgc agatgggcgt gcccatcgtc gtctcgcgca
     3301 gcggcgtcac gcagatgggc cacgcgatgg ccgtccggct gggcctggcg ctgttcggcc
     3361 gggccacgaa ccggcacttc ctctgctact gcggcttcga gcgcttcgac gcgcaacccg
     3421 agccgccgcg cgccgcggcg gccgtcacgc gaccggccga ctgatgaatc cgctcgagcg
     3481 catcgcgccc ggtcgccttg tcgccgggct tgccagggtc ctgcaacgac ggccgatgcg
     3541 ggtcaggcag ggcgttgcag cttcgacagc tcatcgaggg tgttggcgtt gacgaaggca
     3601 tgggcgtcgt cgaactcgac cgttgccgta cgctgcgtcg cggtccacgc gtcgatcttg
     3661 cgcctgccgc tggcgacgaa ccgggaaagc gcgtcgcgca ccgtcgcgcg catcaggcag
     3721 aacaccggct gcaggcaaag tgctccctcc tcgcgcgtcg ccgccatggc gacatcggcg
     3781 tcgtcgcgct cgatgccgcg caccagctgc gccacgagat cgacgggaaa cagcggcgag
     3841 tcgcatggca ccgtcaccag ccacggcgtc gtgcagtgct cgagtccggc caggaatccg
     3901 gccagcgggc cggggtaatc gggcgtcgcg tccggccaga ccggcgagcc catgccggca
     3961 tacgtctcga gatggcg
//
LOCUS       DASRWS010000505         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_791354, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000505 DASRWS010000000
VERSION     DASRWS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791354"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFF72_09480"
     CDS             <1..904
                     /locus_tag="VFF72_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZV93437.1"
                     /translation="TMLALWCICVAATALIARGVAAIAVDPIVEATKSITRWAPDRPA
                     DPAPPADEVGVLMETFNRFRDRVDETVAREREFAANLDHEIRTPLTTIRTDAELIGLE
                     TALPASARQRLERIVASVDEIVATTAAALSSSAGRRVAAEPLVLRDCIGAVCEALADR
                     AAERGLRIEVDLDDGSPLVADRQALTTVCRNIVRNAIEHAAPATLRIAGDVRELRFSD
                     DGPGIAATSLSHVFERYQQGHRGDVGGADAAGVGAASALPRRGLGLAIARRLCDLQGW
                     RLSVRSPVIDGHGTAFRLEFTDDA"
     gene            1072..1836
                     /locus_tag="VFF72_09485"
     CDS             1072..1836
                     /locus_tag="VFF72_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZV93438.1"
                     /translation="MIDGIGPAPPPPFVPGALGGPASIADYVPVHLLLVPAVLVAVAW
                     TLQHGPLDMRIAALFFDPKRGFVGHHLPWLDILGHEAARSLPILVGALSVAAGAAARW
                     RPLWRPWRPILFSIGAAMLAGPLAIAVMKSMTSQHCPTDTLEFGGIVAHALDQAAPFW
                     AATRQAAGRCLPSGHAGGGYALLCLYFAGWAAGRPAWRWGGLAVGVSAGLVFSVVRMM
                     QGAHFASATIWSAAIDWLVCALIFMPLLCRKSPPRN"
     gene            1833..>2170
                     /locus_tag="VFF72_09490"
     CDS             1833..>2170
                     /locus_tag="VFF72_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZV93439.1"
                     /translation="MSAPSAARRRLPAVALLVVVWLVATIGLRPLALPEEGRYVGVAW
                     EMLRSGHWLVPTEDGLPFFHKPPLFYWLDAASMQLFGLHPAAARLPSVLAALLGVAGV
                     RTVAHRWAGE"
BASE COUNT          260 a          816 c          740 g          354 t
ORIGIN      
        1 cacgatgctc gcgctgtggt gcatctgcgt cgcggcgacg gcgttgatcg cgcgcggtgt
       61 cgccgcgatc gccgtcgatc cgatcgtcga ggcaacgaag agcatcaccc gctgggcacc
      121 cgaccggccg gccgatccgg cgccgccggc cgacgaagtg ggggtcctga tggagacctt
      181 caaccggttt cgcgaccgcg tcgacgagac ggtcgcacgc gagcgcgagt tcgcggccaa
      241 cctcgaccac gagatccgca cgccgctcac gaccatccgc accgacgccg agctgatcgg
      301 tctcgagacg gcgctgccgg catcggcgcg ccagcgcctc gagcgtatcg tcgccagcgt
      361 cgatgagatc gtcgcgacga cggcggcggc gctgtcgtcg agtgccggcc gacgcgtcgc
      421 tgccgagccg ctggtcttgc gcgactgcat cggcgcggtc tgcgaggcgc tcgccgaccg
      481 cgccgccgaa cggggcctgc ggatcgaggt cgacctcgac gacgggtcgc cgcttgtcgc
      541 cgaccggcaa gccctcacca ccgtctgccg caacatcgtt cgcaacgcga tcgagcacgc
      601 ggcgccggcg acgctgcgta tcgcgggcga cgtccgcgaa ttgcgcttca gcgacgacgg
      661 gcctggcatc gccgcgacgt cgctgtcgca cgtcttcgag cgctatcagc agggccatcg
      721 cggcgacgtc ggcggcgccg acgcggcggg cgttggcgcg gcgtcggcat tgcctcgacg
      781 cggcctcggc ctggccatcg cccggcggct ctgcgacctg cagggctggc gcctgtccgt
      841 gcgttcgccc gtgatcgacg gccacggcac ggcatttcgc ctcgagttca ccgacgacgc
      901 ctgaacgcaa atctcacgtt tcctgcacgg atttgcgacg agcccgggcc tagcctgcgc
      961 acccgccgtg ccctggcttc gaagcccgag ccaccggcgc cgacggcgac ccatgccctt
     1021 gcacgaccga accgcactcc tcgccgacct tcccggccgc ggtgaacgac gatgatcgac
     1081 gggatcggcc ccgccccgcc gcctcccttc gtgccgggtg ccctcggcgg accggcttcg
     1141 atcgccgact atgtcccggt gcacctgttg ctggtgcccg ccgtcctcgt cgcggtcgcc
     1201 tggaccttgc agcacggccc gctcgacatg cggatcgccg cgctcttctt cgatccgaag
     1261 cggggcttcg tcggccatca cctgccgtgg ctcgacatcc tcggccatga ggcggcgcga
     1321 agcctgccga tcctggtggg cgcgctcagc gtcgccgccg gcgccgcagc ccgctggcgg
     1381 ccgctctggc gaccgtggcg tccgatcctg ttctcgatcg gcgcggccat gctggcgggc
     1441 ccgctcgcca tcgccgtgat gaagtccatg accagccagc actgccccac cgacacgctc
     1501 gagttcggcg gcatcgtcgc tcacgccctc gaccaggcgg cgccgttctg ggcggcgacg
     1561 cgccaagccg ctggacgttg tcttccgagc ggccatgccg gtggcggcta cgcgctgctg
     1621 tgcctctatt tcgccggctg ggcggcgggc cgtcccgcct ggcgctgggg cggcctggcc
     1681 gtcggcgtga gcgcgggcct ggtcttcagc gtcgtgagaa tgatgcaggg cgcccatttc
     1741 gccagcgcga caatctggtc ggccgccatc gactggctcg tctgcgcgtt gatcttcatg
     1801 cccctgctgt gccggaaatc acctcccagg aattgagcgc gccaagcgcg gcccgacgcc
     1861 gccttcccgc agtcgcgctc ctcgtcgtcg tctggctcgt ggcgacgatc ggccttcgtc
     1921 ccctggcgtt gcccgaggag ggccgctacg tcggcgtcgc ctgggaaatg cttcgctcgg
     1981 gccattggct ggtgccgacc gaagacggtc tgccgttctt tcacaagccg ccgctcttct
     2041 actggctcga tgcagcgtcg atgcagctct tcggcctgca tccggcggcg gcccggctgc
     2101 cctcggtgct cgccgcgctg ctcggcgtgg ccggtgtgcg gacggtggcc catcgctggg
     2161 ccggcgaaag
//
LOCUS       DASRWS010000506         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_495202, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000506 DASRWS010000000
VERSION     DASRWS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495202"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFF72_09495"
     CDS             <1..1336
                     /locus_tag="VFF72_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HZV93440.1"
                     /translation="DGGGGIAIVPTAPERQRNSDNDHPYRHDSDFHYLTGFGEPDAWL
                     LVDAAGRSILFCRARDAEREVWDGLRLGPAAAPDRLGVDEAFAIESLDEVVTGRLAGQ
                     PAVWMPGGRADTGARLDRWLEHLRGRERQGVDAPRTVHDLRPVLAEMRLFKDAGEVET
                     MRRAGAISADAHLRAMRFCAARLRRDPRGGVAEYEIEAELLHEFRRRGADGPAYGSVV
                     AAGANACILHYAAGRTELKAGELCLIDAGCELDGYASDITRTFPADGRFTPAQRELYD
                     IVVAAQRAAIDATRPGARQRDAHAAAVGVLAQGMLDTGLLDRHKVGDVDAVVESSAYR
                     AFYMHGTGHWLGRDVHDVGRYDSLAEAPVEQPDWQGGRVVKRPSRRLEPGMVVTIEPG
                     LYVRPGEGVPERYWNIGIRIEDDALVTASGCELISRGVPVEAEAIEAVMRDS"
     gene            1417..>2268
                     /locus_tag="VFF72_09500"
     CDS             1417..>2268
                     /locus_tag="VFF72_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV93441.1"
                     /translation="MPVFLVFAACAFASGFALRLADPIVLPVAHYFAVAPAAAALLNT
                     AYALPYAIAQPFLGPIGDRFGKPRCVQVCIAGLAVMLAAGALATSFAFLIATRVGAGI
                     FAGGLIPLVLAGLGDTYDMSERQVMIGRMLFAIIAGQMLGSVVSGLANEAFGWHSAFF
                     IAAALGATAALIAWLTMARPDPNVEHTRPGSFATLYGRVFANPKAPWLYGCIMVEGAV
                     FYGLFPYMGELLLATTAPGSRTISVETGLVLGAFGIGGLIYAASVRRLLRVFGMRRMC
                     LIGSSCAA"
BASE COUNT          283 a          848 c          797 g          340 t
ORIGIN      
        1 cgacggcggc ggcggcatcg ccatcgtgcc gacggcgccc gaacggcagc gcaactccga
       61 caacgaccat ccgtaccggc acgacagcga cttccactac ctgaccggct tcggcgagcc
      121 cgacgcgtgg ctgctcgtcg atgccgccgg ccgctcgatc ctgttctgtc gcgccaggga
      181 cgccgagcgc gaggtctggg acggactgcg cctcggcccc gcggcggcgc cggacaggct
      241 cggcgtcgac gaggccttcg cgatcgaatc cctcgacgag gtcgtgaccg ggcgcctcgc
      301 cggtcagccg gccgtatgga tgccgggtgg ccgcgccgac acgggcgccc gcctcgaccg
      361 ctggctcgag cacttgcgag gccgcgaacg ccagggcgtc gacgcgccgc gcacggtgca
      421 cgacctgaga ccggtgctcg ccgagatgcg tctcttcaag gatgccggcg aggtcgagac
      481 gatgcgccgc gccggggcga tcagcgccga cgcccacctg cgcgcgatgc gcttttgcgc
      541 cgcccggctg cgccgcgatc cgcgcggcgg cgtcgccgag tacgagatcg aggccgagct
      601 gctgcacgaa ttccggcgtc gcggcgccga cggcccggcg tacggatcgg tggttgccgc
      661 cggcgccaac gcctgcatcc tccattacgc ggcgggccgc accgagctga aggcgggcga
      721 gctctgcctg atcgacgccg gctgcgagct cgacggctat gcaagcgaca tcacgcggac
      781 ctttccggcc gatggccgct tcacgccggc gcagcgcgag ctctacgaca tcgtcgtcgc
      841 cgcccagcgc gcggcgatcg acgcgacgcg tccgggggcg cgccagcgcg acgcccatgc
      901 cgccgccgtc ggcgtcctgg cccagggaat gctcgacacc gggctgctcg atcgccacaa
      961 ggtcggcgac gtcgatgccg tcgtcgagtc gtcggcgtac cgcgccttct acatgcacgg
     1021 caccggccac tggctcggcc gcgacgtgca cgacgtcggc cgctacgact cgctcgccga
     1081 ggcgcccgtc gagcagcccg actggcaggg cggccgcgtc gtcaagcggc cgtcgcgccg
     1141 gctcgagccg ggcatggtgg tgacgatcga gcccggcctg tacgtgcggc cgggcgaagg
     1201 cgtccccgag cgctactgga atatcggcat ccgcatcgaa gacgacgcgc tggtcacggc
     1261 gagcggctgc gagctgatca gccggggcgt tccggtcgag gccgaggcga tcgaggccgt
     1321 gatgcgcgac tcgtgaaggc aaccgggccg gcccgcgatc cggttgcatc cggcctcgac
     1381 gcgagcgcca cggacggctg aggaagatct cttcccgtgc cggtctttct cgtcttcgcc
     1441 gcctgtgcct tcgccagcgg cttcgcgctg cgccttgccg atccgatcgt cctgccggtg
     1501 gcccactact tcgcggtcgc gccggcggcg gcggccctgc tcaacaccgc ctacgcactc
     1561 ccctacgcga tcgcgcagcc cttcctcggg ccgatcggcg accggttcgg caagccgcgc
     1621 tgcgtgcagg tctgcatcgc cggcctcgcg gtgatgctgg cggccggcgc cctcgcgacg
     1681 agctttgcct tcctcatcgc cacccgcgtc ggcgccggca tcttcgccgg cggcctgatt
     1741 ccgctcgttc tcgccggcct cggcgacacc tacgacatga gcgagcgcca ggtgatgatc
     1801 ggccggatgc tgttcgcgat catcgccgga cagatgctcg gctcggtcgt ctcgggcctg
     1861 gccaacgagg ccttcggctg gcacagcgcg ttcttcatcg ccgcggcgct cggcgcgacg
     1921 gccgcgctca tcgcgtggct gacgatggcg cggcccgatc cgaacgtcga gcacacgcgc
     1981 ccgggctcgt tcgccacgct ctacggccgc gtcttcgcca atccgaaggc gccctggctg
     2041 tacggctgca tcatggtcga gggcgccgtc ttctacgggc tctttccgta catgggagag
     2101 ctgctgctgg cgacgaccgc gcccggcagc cggacgatct ccgtcgagac cggcctggtc
     2161 ctgggcgcct tcggcatcgg cggcctgatc tacgcggcga gcgttcgccg cctgctgcgc
     2221 gtgttcggca tgcgccggat gtgcctgatc ggctcgtcgt gcgcggcg
//
LOCUS       DASRWS010000507         6364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3397108, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000507 DASRWS010000000
VERSION     DASRWS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397108"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFF72_09505"
     CDS             <1..788
                     /locus_tag="VFF72_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93442.1"
                     /translation="STGMSEPVAAPRGNSAPPVDAGLALRVRDLVAGYTPGVDILRGV
                     SIDVRSGEIVTVLGPNGAGKSTLIKAIAGLVPVRTGGIELFGADISGVEAHRMVRLGL
                     AYVPQVSNIFAKLTVEENLEMGALHHRAAQQQNIERMYGLFPRLKERRRQAAGTMSGG
                     ERQMVAVARALMAEPRVLMLDEPSAGLSPKLVGVVFGKIREVRDSGVTILMVEQNARA
                     ALAISDRGYVLAEGRQQVSGGARELLADPEVGALYLGVTRGLA"
     gene            785..1678
                     /locus_tag="VFF72_09510"
     CDS             785..1678
                     /locus_tag="VFF72_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93443.1"
                     /translation="MIAQYFADGVILGATLSLGAIGLTLTYDILRFANFAHGEFLTFG
                     AYFALVFASLFVGGRTLGPLTFGWSFVLALVVAVGLTAGLALLLDWLLFRRLRKSDIA
                     VTLVIASFGASLMLRNVVVFAWGSQPEYYTRTIAIAREVLPGVRLTANEIFVIVLTAV
                     LMVALHFFLTGTRLGKSMRAVSDNAPLARVTGIDVQGVIRWTWIVGGGLAAVAGVLYG
                     LTVQIAPEMGFNLILPMFAAAILGGIGSIYGAVLGGLVIGIAESVSVPFIGAAYMPAV
                     GFALMFLILLVYPKGILGART"
     gene            1675..2682
                     /locus_tag="VFF72_09515"
     CDS             1675..2682
                     /locus_tag="VFF72_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93444.1"
                     /translation="MSGIGSYLVFFLIQALIFAVVCLGLNLQWGFTGLFNIGVSGFYL
                     VGAYAFAIICGPPYASHLGGFGLPFVVGLFGAVVASGAIAFILGIPTLRLREDYLAIV
                     TIGIGTVLQLVALNTASLTGGSQGTTSIPRPLPDLLQGVLQRNLLMLGLMVVVTLVVF
                     GALEAMVRSPWGRVLKAIREDEAAASSLGKNPFAFRLQSFVIGSAIMGLGGALYASFI
                     GFVSPEDFMPILTFQIWTMLIVGGSGNNKGAVVGALVMWAVWTLSGSAAQVLLPADLQ
                     VKGGAAQIILIGLVLMLTLVLRPRGLLGEEAVVSRGARGAPLPRRQAPRRRPLRQPSD
                     S"
     gene            2873..3160
                     /locus_tag="VFF72_09520"
     CDS             2873..3160
                     /locus_tag="VFF72_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HZV93445.1"
                     /translation="MMSLVASGGVIATGAARAAAVVSESDAQAVALGYKVNTREVDQK
                     KYPKHDVSQHCGDCQLYQGKRDDPKATGPCPIFGEKLVHQTGWCSAWVKMA"
     gene            complement(3276..3632)
                     /locus_tag="VFF72_09525"
     CDS             complement(3276..3632)
                     /locus_tag="VFF72_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93446.1"
                     /translation="MNAMQAARDAVVRQARALWSWRQATLEGAGRSVSMSDGGEAPFT
                     GHGLVVTAAQDLGFFARSSTWRCTRCSRVHRFSDRVPVIAASPCRCASIEFEPHRDGV
                     FATPILSDGTTPAIPA"
     gene            complement(3784..4326)
                     /locus_tag="VFF72_09530"
     CDS             complement(3784..4326)
                     /locus_tag="VFF72_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HZV93447.1"
                     /translation="MTHRTLVATLLDEASFAPYGDVIDRRSVAADVSINGGTARRTSD
                     LARVDASPRGGHVALSLVRALPRPLPMRLECMERHLRSSQAFVPLDATRWLVVVAARG
                     RAPAVRRLRAFLASGEQGVNYARGTWHHPLIALDRECRFLVVDRIADDGAEDCEVWPL
                     AGLEVNVPLDFTEPHAAADR"
     gene            complement(4409..4603)
                     /locus_tag="VFF72_09535"
     CDS             complement(4409..4603)
                     /locus_tag="VFF72_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93448.1"
                     /translation="MAVRFCRRPAAPAHHAPEHGGASSSLRRWTISLAKHDKIAQGDL
                     DDTVGAVAGIEMNEIMKALQ"
     gene            4602..5183
                     /locus_tag="VFF72_09540"
     CDS             4602..5183
                     /locus_tag="VFF72_09540"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZV93449.1"
                     /translation="MHSLRLACLLVLAGTSATASAALQPGDAAPPFTAQASLAGKPFA
                     YSLAAQLARGPVVVYFYPAAYTQGCDIEAHEFSSRIDRFTAAGASVVGVSLDRIERLN
                     AFSADPDYCAGKLAVASDADGRIARSFHLQVHAAQGGAKDTRGQEIGHGFAQRTTFVI
                     RRDGTVAATVGGVAPDENVRRTLEVVEQLAARR"
     gene            complement(5241..6221)
                     /locus_tag="VFF72_09545"
     CDS             complement(5241..6221)
                     /locus_tag="VFF72_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZV93450.1"
                     /translation="MTRTTEAPAITRSDIAVARSRIAADVRHTPLMRLRGSDLGIDCA
                     ELWLKLEHLQAGGSFKARGMFNRMRSNPVPEAGVVIASGGNAGIAVAVAAKAMGVRCE
                     VFVPETTSAAKRARLDELGAVLVVGGASYAEALAASLERQAATGALLMHAYDQREVVI
                     GAGTLAAEVEDDAGVPDRILVSVGGGGLIAGIAAWFEDRCAIDALEPERAPTLYRARE
                     AGKPVDVDVAGIAADSLGARRVGAIAWELAQQYVTGAWLLADADIRAAQEAMWSGLRL
                     AVEPAAALPLAALATRTVAPRRHERVLLVICGANLDLASLATPPRPSPTS"
BASE COUNT          869 a         2210 c         2242 g         1043 t
ORIGIN      
        1 ggagtaccgg catgagcgag cccgtggccg cgcctcgcgg caacagcgct ccgccggtgg
       61 acgccggcct ggccttgcgc gtgcgcgacc tcgtcgccgg ctacacgccc ggggtcgaca
      121 tcctgcgcgg cgtttccatc gacgttcgca gcggcgagat cgtcacggtg ctcgggccca
      181 acggcgccgg caagtcgacc ctgatcaagg ccatcgccgg gctcgtcccg gtgcgcaccg
      241 gcggcatcga gctcttcggc gccgatatct ccggcgtcga ggcgcacagg atggtgcgcc
      301 tcggcctggc ctacgtgccg caggtgagca acatcttcgc caagctcacg gtcgaggaga
      361 acctggagat gggcgcgctg caccaccggg ccgcgcagca gcagaacatc gaacgcatgt
      421 acgggctctt tcctcgcctg aaggaacggc gacgccaggc ggccggcacg atgtcggggg
      481 gcgagcggca gatggtggcg gtcgcccgcg cgctgatggc cgagccgagg gtgctgatgc
      541 tcgacgagcc atcggccggc ctttcgccca agctggtcgg cgttgtcttc ggcaagatcc
      601 gcgaggtccg cgacagcggc gtgacgatcc tgatggtcga gcagaacgcc agggcggcgc
      661 tcgccatctc cgaccgcggc tacgtgctcg ccgagggccg ccagcaggtg agcggcggtg
      721 cgcgcgaatt gctcgccgac ccggaagtcg gcgcgctgta cctgggcgtg acgcgggggc
      781 tcgcatgatc gcgcagtact tcgccgatgg cgtcattctc ggggccacgc tctcgctcgg
      841 cgcgatcggc ctcaccctca cctacgacat cctgcgcttc gcgaatttcg cgcacggcga
      901 gttcctgacc ttcggcgcct acttcgcgct cgtcttcgcc agccttttcg tcggcggccg
      961 cacgctcggc ccgctgacct tcggctggag cttcgtgctg gcgctcgtcg tcgccgtcgg
     1021 gctcactgcg gggctcgccc tcctgctcga ctggctgctc tttcgccggc tgcgcaagag
     1081 cgacatcgcg gtcacgctgg tgatcgcctc gttcggcgcg tcgctgatgc tgcgcaacgt
     1141 cgtcgtcttc gcctggggct cgcagcccga gtactacacg cgcacgatcg cgatcgccag
     1201 ggaagtgctg cccggcgtgc gtctcaccgc caacgagatc ttcgtcatcg tgctcaccgc
     1261 ggtgctcatg gtcgcgctgc atttcttcct gaccggcacc cggctcggca agtcgatgcg
     1321 cgcggtttcc gacaatgcgc cgctcgcgcg cgtcaccggc atcgacgtgc agggggtgat
     1381 ccgctggacc tggatcgtcg gcggcggcct cgcggcggtg gccggcgtgc tgtacggcct
     1441 gacggtgcag atcgcgccgg agatgggatt caacctgatc ctgccgatgt tcgccgccgc
     1501 gatcctcggc ggcatcggca gcatctacgg cgccgtgctg ggcgggctgg tgatcggcat
     1561 cgccgagtcg gtctcggtgc ccttcatcgg cgccgcctac atgccggcgg tgggcttcgc
     1621 gctgatgttc ctgatcctgc tcgtgtatcc gaaaggcatc ctcggagcgc gaacgtgagc
     1681 ggcatcggct cgtacctggt gttcttcctg atccaggcgc tgatcttcgc agtggtctgc
     1741 ctcggcctga acctgcaatg gggcttcacg gggctcttca acatcggcgt gtccggtttt
     1801 tacctggtcg gcgcctacgc gttcgcgatc atctgcgggc cgccctatgc ctcgcacctc
     1861 ggcggcttcg ggctgccttt cgtcgtcggc ctcttcggcg cggtcgtcgc ctcgggggcg
     1921 attgccttca tcctcggcat tccgacactg cggctgcgcg aggactacct ggcgatcgtc
     1981 acgatcggca tcggcaccgt gctgcagctc gtcgcgctga acaccgcttc gctgaccggg
     2041 ggcagccagg gcacgacgag cattccgcgc ccgctgcccg acctgctgca aggcgtgctg
     2101 cagcgcaatt tgctgatgct cgggctgatg gtcgtcgtca cgctcgtcgt cttcggcgcg
     2161 ctcgaggcga tggtccgctc gccctggggg cgtgtgctga aggcgatccg cgaggacgag
     2221 gccgccgcct cgtcgctcgg caagaacccc ttcgctttcc ggctccagtc gttcgtgatc
     2281 ggctcggcga tcatgggtct cggcggcgcg ctctacgcga gcttcatcgg cttcgtgagc
     2341 cccgaggact tcatgccgat cctgaccttc cagatctgga ccatgctgat cgtgggcggc
     2401 agcggcaaca acaagggcgc tgtcgtcggc gcgctggtga tgtgggcggt gtggacgctc
     2461 agcggcagcg ccgcccaggt gctcctgccg gcggatctgc aggtcaaggg cggggcggcg
     2521 cagatcatcc tgatcgggct cgtgctgatg ctgacgcttg tcttgcggcc gcgtggactg
     2581 ctcggcgagg aggcggtggt ttcgaggggt gcgcgtggcg cgccattgcc gcgccggcag
     2641 gcgccacgcc ggcggcctct tcggcagccg tccgattcgt gaggacgggt ccgggttggc
     2701 ctcgccgtcg acttcactgg ctgcgggaac gtcgtttgct ggcatggaca cgccgcgacg
     2761 acgcgctgct cgcggcatcg gtccggcgcc tcgccagtcg cccgcaccgg ccgataccgt
     2821 tcagcacttc tccaggaggt ttgccgtgag ttcttcgaat cgtcgcgtct tcatgatgag
     2881 tcttgtcgcc agcggcggcg tgatcgccac aggcgcggcg cgggccgcgg ctgtcgtgtc
     2941 ggaatccgac gcacaggcgg tcgcgctcgg ctacaaggtc aacaccaggg aagtcgatca
     3001 gaagaagtat ccgaagcacg acgtcagcca gcactgcggc gattgccagc tctaccaggg
     3061 taagcgcgac gatcccaagg ccaccggccc ttgcccgatc ttcggcgaga agctggtgca
     3121 ccagaccggc tggtgcagcg cctgggtgaa gatggcctga gcctcccgcg gcgggatctc
     3181 gccacggcac cgactctggc cggctgagca agacggcgcc tcgccgattc ggcgtcgggc
     3241 cgcgtgatcc ggttcagtgt ggctcgcgag ccgggctacg ccggaatcgc cggcgtcgtc
     3301 ccgtcggaca ggatgggcgt ggcgaacacg ccgtcgcggt gcggttcgaa ctcgatgctg
     3361 gcgcaccggc aaggcgacgc ggcgatcacg ggcacccggt cggagaaacg gtgcacgcgc
     3421 gagcatctcg tgcagcgcca ggtgctgctt cgcgcgaaga agccgaggtc ctgcgcggcg
     3481 gtgacgacca gcccatggcc tgtgaacggc gcctcgccgc cgtcgctcat cgagaccgac
     3541 ctcccggcgc cctcgagcgt ggcctgtcgc cacgaccaca gcgcccgggc ctgccggacg
     3601 accgcgtcgc gcgcagcttg catcgcattc atgccaatcc ctcgtctggt gcgtcggtga
     3661 cgatagcgga cggcgcgccg gcgcggcctg ccgttcttcc tgaaccccgc gtttcagggg
     3721 ggtcgtgaag cgcaggcatg catacgttgc gcggccgagt gccgaggcga cccggcgagc
     3781 gcgtcagcga tcggccgcag cgtgcggttc ggtgaaatcc aggggcacgt tcacctccag
     3841 gcccgccaag ggccagacct cgcaatcctc ggccccgtcg tcggcgatgc ggtcgacgac
     3901 gagaaatcgg cactcgcgat cgagggcgat cagcgggtgg tgccaggtac cgcgagcgta
     3961 gttgacgcct tgctcgccgc tcgcgagaaa ggcgcgcaga cgccggacgg ccggagcgcg
     4021 tccgcgtgcg gcaacgacga cgagccagcg cgttgcgtcg agcgggacga aggcctggct
     4081 cgagcgcagg tggcgctcca tgcactcgag acgcatcggc agcggccggg ggagggcgcg
     4141 gaccaggctg agggccacat ggccgccgcg cggggaggcg tcgacgcgcg ccaggtcgct
     4201 cgttcgccgc gccgtgccgc cattgatcga gacgtcggcc gcgacgctgc gtcgatcgat
     4261 gacgtcgccg tacggcgcga aggaagcttc gtcgagcagc gttgcgacga gcgttcgatg
     4321 cgtcaccggc cgagccccgc gcgattcacc cggacgcgcg tgctgcgcga tcgccgagcg
     4381 tcggggtgca caggcatcag gggagatgtc actgcagcgc cttcatgatc tcgttcatct
     4441 cgatgccggc gacggcgccg accgtgtcgt cgaggtcgcc ctgtgcgatc ttgtcatgct
     4501 tcgccaggga aatcgtccac ctccttagcg acgacgacgc gccgccgtgc tcaggtgcat
     4561 gatgcgcggg tgccgcaggg cggcgacaga aacggaccgc catgcacagt ctccgccttg
     4621 cctgccttct cgttctcgcg ggcacgagcg ctacggcgtc ggccgccttg cagccgggtg
     4681 acgcggcgcc gccgttcacg gcacaggcct cgctggctgg caagccgttc gcctattcgc
     4741 tggccgccca gctggccaga ggccccgtcg tcgtctactt ctaccccgcg gcctacacgc
     4801 agggatgcga catcgaggcg cacgagttct cttcgcgcat cgaccggttc actgccgccg
     4861 gtgcctcggt ggtcggggtg tcgctcgacc gcatcgagcg gctcaacgcc ttctcggccg
     4921 accccgacta ttgcgccggc aagctcgccg tcgcttccga tgccgacggc cgcatcgccc
     4981 ggtcgtttca cctccaggtg cacgctgcgc aaggcggcgc caaggacacc cggggccagg
     5041 agatcggcca tggcttcgcc cagcgaacga cattcgtcat ccgtcgcgac ggcacggtcg
     5101 cggcgacggt gggcggagtg gcgcccgacg aaaacgtcag gcgcacgctc gaggtcgtcg
     5161 agcagttggc cgcacgtcgc tgattcgcgc ccggcgcgac aagagagcgg gagagcgatc
     5221 gaccagatcc gttcgatcgg tcacgacgtg ggggacgggc gaggcggcgt cgcgagtgac
     5281 gcgaggtcga ggttggcgcc gcagatcacg agcaggacgc gttcgtggcg ccgcggcgcg
     5341 acggttcgcg tcgccagcgc ggccagcggc agcgccgccg cgggctcgac cgccaggcgt
     5401 agcccgctcc acatcgcctc ctgcgcggcc cggatgtcgg cgtcggcgag cagccaggct
     5461 cccgtgacgt attgctgcgc cagctcccag gcgatcgcac cgacgcgccg tgcgccgagc
     5521 gaatcggcgg cgatcccggc gacgtcgacg tcgaccggct tgcccgcctc gcgggcgcga
     5581 tagagcgtcg gcgcccgctc gggctcgagc gcgtcgatcg cgcatcgatc ctcgaaccac
     5641 gccgcgatcc cggcgatcag cccgccgccg ccgacgctca cgaggatgcg atcgggcacg
     5701 ccggcatcgt cttccacttc ggcggcgagc gtgccggctc cgatcacgac ctcgcgctgg
     5761 tcgtacgcgt gcatcagcag cgcgccggtc gcggcctgtc gctcgaggct cgcggccagc
     5821 gcctcggcat agctcgcccc gccgacgacg agcaccgcac ccagctcgtc gaggcgggcg
     5881 cgcttggcgg cgctcgtcgt ctcgggcacg aagacctcgc agcgcacacc catcgccttc
     5941 gccgcgacag ccacggcgat gccggcgttg ccgcccgacg cgatgacgac gcccgcttcg
     6001 ggaacagggt tcgagcgcat gcggttgaac atcccgcggg ccttgaagct gccgcccgcc
     6061 tgcaggtgct cgagcttcag ccacagctcg gcgcaatcga tgccgaggtc gctgccgcga
     6121 agtcgcatga gcggggtgtg ccgcacgtcg gcggcgatgc gcgagcgggc gacggcgatg
     6181 tcggagcgcg tgatggcggg ggcttcggtg gttcgggtca tggatgaggg cagcgtaacg
     6241 caggccgtcc agccacgcgg cgaaaccggg aatacggccc gataagcggg ccagttcgac
     6301 gctttggcgc gttcgggccc atgtagattc gttcgatccc gcaccgccgt gccttgtttg
     6361 cgca
//
LOCUS       DASRWS010000508         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2789110, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000508 DASRWS010000000
VERSION     DASRWS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789110"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3392
                     /gene="treY"
                     /locus_tag="VFF72_09550"
     CDS             <1..>3392
                     /gene="treY"
                     /locus_tag="VFF72_09550"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZV93451.1"
                     /translation="AEQQLAHAAAHGRERGMAVGLYLDLAVSVDRAGADTWSHASTYA
                     LTASVGAPPDAFNQQGQAWGLPPLRPDRLRVGGYRAFIDTLVANMRLAGALRIDHVMG
                     LMRLFWIPAGRGARDGAYVHYRVDELLAIVVLESHRHRCLVIGEDLGTVLDEMRAALR
                     ERGVLSYRLLYFERDAEGGFKAPDDYPRDALVAVATHDLPTLAGWWQGRDIDLRRDLG
                     LFADPDGEAAQRAERAQDRQRLLAVLASDAAAPEAEAGRGDDRARPGRAAGAAAGGGA
                     GGRSGADLALGRPVFDAAAPMTAPLAEAIHRHLAATPSVVMMVQLEDALGMVEEANLP
                     GTTDQQPNWRRKLVADLDAIGASVRVDSLCAAIRSRRPTPAAQSAPVASVADVVEVVE
                     VAQVARVAQAAEVGQVIPGPPAAPAVQAATVAPATASPSPPDGITVAPRIPRATYRLH
                     LHGGFDFDAAARVLPYLVRLGVSHVYCSPISRAAPGSLHGYDVVAHDEINPELGGAAG
                     LARFVAALREHGLGLLLDLVPNHMGTGAENAWWADVLENGEASLLAHYFDIDWHPLDA
                     DLDGKVLLPVLGDAYGDVLDRGELGIDFDAARGSFAVAYHEHRFAIDVRDYATLLRLL
                     EIDDAALRDAVTTLAAAFAGLPSRHALGDASVDRRARDKVALKERLARLASEHPALRA
                     AIERALAEAGAPERLHDLLEGQAWRLASWRVASDEINYRRFFDVNSLAALRIERRPVF
                     EATQGLALELTATGVADGLRIDHPDGLRDPARYFLRLQQGHAERAGLVVAEPPGGRPA
                     RPLYLLAEKIAALDETIPGSWAVHGTTGYRFASLVNGLFVDTAAEARLDRIWRAFSGD
                     PRSFDEHAYTGKRDVMRSGLASELTVLASELLRIARADRRSRDHTFNSLRDALAEITA
                     CLAVYRTYIVDAASTQDRRYVDQAVSRARHRSPAADAAVFDFVRRTMLGEAGDGATAA
                     LRERALHWAVRFQQFSAPVAAKGVEDTADYRYLRLASLNEVGGDPARFGTTVREFHRA
                     SADRAAHRPHTLIATSTHDHKRSADVRCRIDVLSEMPAGWRLLLRRWSRLNRRHKTRL
                     DDGGWAPSPRDENLLYQTLLGTLPPAPLDAPARARYRERIQA"
BASE COUNT          451 a         1301 c         1178 g          462 t
ORIGIN      
        1 tgccgagcag cagctcgcgc acgccgcggc gcacggccgc gagcgcggca tggcggtcgg
       61 tctctacctc gacctcgcgg tgtcggtcga tcgtgccggc gccgacacct ggtcgcacgc
      121 gtcgacctac gcgctgacgg ccagcgtcgg cgcgccgccc gatgccttca accagcaagg
      181 ccaggcctgg ggcctgccgc cgttgcgtcc cgaccggctg cgcgtgggcg gctatcgggc
      241 gttcatcgac accctcgtcg ccaacatgcg cctggccggc gccttgcgca tcgaccacgt
      301 gatggggctg atgcggctgt tctggattcc ggccgggcgc ggcgcgcgcg acggtgcgta
      361 tgtgcactac cgcgtcgacg agctgctcgc catcgtcgtc ctcgaaagcc atcggcaccg
      421 ctgcctcgtg atcggcgagg accttggcac tgtgctcgac gagatgcgcg ccgcgctgcg
      481 cgagcgtggc gtgctgtcgt accggctcct ctatttcgag cgcgacgccg aaggcggatt
      541 caaggcgccg gacgactatc cgcgcgacgc gctggtcgcc gtcgcgaccc acgatctgcc
      601 gacgctcgcg ggctggtggc agggtcgcga catcgacctg cggcgagacc tcggcctgtt
      661 cgccgacccg gacggcgagg cggcgcagcg ggccgagcgg gcacaggatc gccagcgcct
      721 gctcgcagtg ctcgccagcg acgcagcagc gccagaagca gaagccggcc gcggcgacga
      781 tcgtgcccgt cccggtcgcg ccgccggggc ggccgcgggc ggcggcgccg gcggtcgcag
      841 tggcgcggac cttgctctcg ggagaccggt cttcgacgcg gcggcgccga tgaccgcgcc
      901 gctcgccgag gcgatccatc gccatctggc ggcgacgccg tcggtcgtca tgatggtgca
      961 gctcgaggac gcgctcggca tggtcgagga agccaacctg ccgggaacca ccgaccagca
     1021 accgaactgg cgccgcaagc tcgtcgccga cctcgacgcg atcggagcgt cggtgcgcgt
     1081 cgactcgctg tgtgcggcca tccgctcgcg gcgtccgacg ccggccgcgc agagcgcacc
     1141 tgtcgcgtca gtcgcagacg tcgtcgaggt cgtcgaagtc gcacaggtcg cacgggtcgc
     1201 acaggccgca gaggtcggac aggtcatacc gggcccacca gccgcaccgg ccgtacaagc
     1261 cgccacggtc gcaccggcca cggcatcgcc ttcgccgccg gacggcatca ccgtcgcgcc
     1321 acgcatcccc cgcgcgacct atcgtctgca tctgcacggc ggcttcgatt tcgacgcggc
     1381 agcgcgcgtc ctgccgtacc tcgtgcgtct cggcgtcagc cacgtctact gctcgccgat
     1441 cagccgcgcc gcgccgggca gcctccacgg ctacgacgtc gtcgcccacg acgagatcaa
     1501 tcccgagctc ggcggcgccg ccggcctggc gcggttcgtc gcggcgctgc gcgagcacgg
     1561 cctggggctg ctcctcgacc tcgttcccaa ccacatggga accggggccg agaacgcctg
     1621 gtgggccgac gtgctcgaga acggcgaggc ctcgctgctt gcgcactact tcgacatcga
     1681 ctggcatccg ctcgacgccg atctcgacgg caaggtgctg ctgcctgtgc tcggcgacgc
     1741 gtacggcgac gtcctcgacc gcggcgagct cggcatcgac ttcgatgcgg cgcgcggcag
     1801 cttcgcggtg gcctaccacg agcatcgctt cgcgatcgac gtgcgcgact acgcgaccct
     1861 gctgcgattg ctcgagatcg acgatgccgc gctgcgcgac gccgtcacca cgctcgccgc
     1921 ggccttcgcc ggcctgcctt cccgacacgc cctgggcgat gcgtccgtcg accggcgggc
     1981 tcgcgacaag gtggcgctga aggagcggct cgcccgtctc gccagcgagc atccggcact
     2041 gcgggcggcg atcgagcgcg cgctcgccga ggccggcgcc cccgagcgac tgcacgacct
     2101 cctcgaaggg caggcctggc gactcgcctc gtggcgcgtc gccagcgacg agatcaacta
     2161 ccggcgcttc ttcgacgtca actcgctcgc cgcgctgcgc atcgagcggc gcccggtctt
     2221 cgaggcgacg caggggctgg cgctcgagct caccgcgacc ggcgtcgccg acggcctgcg
     2281 catcgaccat cccgacggcc tgcgcgaccc ggcccggtac ttcctgcgcc tgcagcaggg
     2341 ccacgccgag cgcgccgggc tcgtcgttgc cgagcccccc ggcggccggc cggcgcggcc
     2401 gctctacctg ttggccgaga agatcgccgc cctcgacgag acgattcccg gatcgtgggc
     2461 cgtccacggc accaccggct accgcttcgc gtccctcgtc aacggccttt tcgtcgacac
     2521 cgcggccgag gcacggctcg accggatctg gcgggccttc agcggcgatc cacgcagctt
     2581 cgacgagcac gcctacaccg gcaagcgcga cgtcatgcgc agcggcctcg cgtcggagct
     2641 gaccgtgctg gcgagcgagc tgctgcgcat cgcccgcgcc gaccggagga gccgcgacca
     2701 caccttcaac tcgctgcgcg acgcgctcgc cgagatcacg gcctgcctcg cggtgtatcg
     2761 cacctacatc gtcgacgccg cgtcgacgca ggaccggcgc tacgtcgacc aggccgtgag
     2821 ccgggcccgg caccgaagcc ccgccgccga tgcggcggtg ttcgacttcg ttcgccggac
     2881 gatgctgggc gaggcgggcg acggcgcgac cgcggcgctg cgcgagcgcg cgctgcactg
     2941 ggccgtgcgt ttccagcagt tcagcgcccc ggtcgcggcc aagggtgtcg aggacaccgc
     3001 cgactatcgc tacctgcggc tcgcctcgct caacgaggtc ggcggcgatc cggcgcgctt
     3061 cggcaccacg gtgcgcgagt tccatcgcgc cagcgccgac cgcgccgcgc atcggccgca
     3121 cacgctgatc gcgacctcga cccacgacca caagcgctcg gccgacgtgc gctgccggat
     3181 cgacgtgctc tcggagatgc cggccggatg gcgactgctg ctgcgccgct ggtcgcggct
     3241 caaccggcgc cacaagacgc ggctagacga tggcggctgg gcgccgtcgc cgcgcgacga
     3301 gaacctgctc taccagacgc tgctcggcac gctgccgccg gcgccgctcg acgcgccggc
     3361 gcgcgccagg tatcgcgagc gcatccaggc ct
//
LOCUS       DASRWS010000509         5755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3397743, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000509 DASRWS010000000
VERSION     DASRWS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397743"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1028
                     /locus_tag="VFF72_09555"
     CDS             168..1028
                     /locus_tag="VFF72_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZV93452.1"
                     /translation="MDWTERRERLRSLIAGPRCIYPGSVFDAISARIAEDLGYEAGMF
                     AGSIASFAVLGAPDVVVLTLTEFAQQAYRINRAGKLPLLVDADHGYGNALSVKRTVEE
                     LETAGVAAMTIEDTALPTAYGASGPNLIPIAEGVGKMKAALAGRQDPLLAIAGRTSAI
                     QITGLDDAIARGKAYQEVGVDALFFVGVKTRAELDALSAATRIPIILGGATPELTDRE
                     YLASRRVRIALQGHQPFAAGVKAVYETLKALREGTPPGKLQGVADADLMKRVTRDADY
                     AKWTKGFLGG"
     gene            1039..2007
                     /locus_tag="VFF72_09560"
     CDS             1039..2007
                     /locus_tag="VFF72_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93453.1"
                     /translation="MRRRAFLATGALALATTPLRAQRLARVAFLGVQAEATQDPRQIE
                     QFKAGLAENRLVDGRTVAVEYHWADGRPERLRALARALGARSDLDVIVTVGSQAVREL
                     MATGTRIPIVLAVIGDPVRPGFVKTLARPGGTITGLSMSNSELEGKRLDILRAAVPSI
                     ARVMVLLDPGMEGEGVDVVERNARSLGIELLMTPVANAGQLAPGFASAAGRGADSLVT
                     MASPFLNFQRDRIIALANQHRLPSIWEVDTFTRAGGLMSYGPSFADMYRRSAGFVAKI
                     LKGANPGDLPVEQPTRFELVVNMRTARLLGLTFSQSFLARADELIE"
     gene            2004..2984
                     /locus_tag="VFF72_09565"
     CDS             2004..2984
                     /locus_tag="VFF72_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93454.1"
                     /translation="MIKRRFLMASGLAAVSSRSVRAQRPPLIGYLGGRSLATDAHLLQ
                     AVKDGLKENGFVEGRNVKFEFRWADGDFSRLDRLAKELVALKPDLLMAVGGTPVPIAM
                     ERARGSLAIPLVFTIGFDPVSLKLVDSLARPGHDATGAMLLANALEGKRLDLLKDMQP
                     GLQSAALLVNSTSAMVNQLEADAQRAAAALGVALHFVKVSTVAELDAALADLAPSKPG
                     ALAVSIDGFLIAQRRRIIDWAARERVPAIYPNREFTEAGGLASYAARWAEAYHWAGVY
                     AGRILRGAKAGDLPIQQPTAYELVVNLKTARAQGITLPAAFTARADEVIE"
     gene            complement(3009..3887)
                     /locus_tag="VFF72_09570"
     CDS             complement(3009..3887)
                     /locus_tag="VFF72_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZV93455.1"
                     /translation="MHYLLSHRPTYRYASTVDSAHHIAHLRARSFPGQTVTSIGVTAQ
                     PEPSMATQHVDHFGNLIDLLRIDQPHRRFDIEVRAAIEVRFPVPPPADQTPAWEEIAR
                     ALDGNGFPKPIEASEFVHDSPLVPIDEELAAYGARSFTPGRPILAAARELTTRIKADF
                     EYHPGATDISTALHEVFAGKAGVCQDFAHVMIAALRAHGLAAAYVSGYIRTVPSAEEL
                     ALRGADASHAWVAVWCGERAGWVHLDPTNDLVAHEDHVAVAWGRDFSDVSPLRGVILG
                     GGSHAYSVAVTLVPTG"
     gene            complement(3887..>5755)
                     /locus_tag="VFF72_09575"
     CDS             complement(3887..>5755)
                     /locus_tag="VFF72_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZV93456.1"
                     /translation="PHGMSNPNIAVLTPGSLSATYFEHVYLSRALGVSLVEGGDLVAR
                     DGEVLIKTLAGLRPVHMLLRRLDSSFADPLELRADSALGVTGLVEATRTAKIAVANAL
                     GSGLVETPALMPFLYGISQRLLGQPLKLPSLDVWWLGEPPAKDYVLANLDAMIVRPCL
                     GPDREPIVVGMLEGEARHRLRQRLSANPGHFVAQSPVSPSLNPKWDGAQLAPSAVVLR
                     CFLVSNGASWRVLPGGLAREPAGNTALGQLGRLSGTLKDVWVLAEDAADVQIPASRRF
                     HPLAVDRGGADLQSRVADNLYWLGRYVERLDDNARLLRLTVTRVAQGVIGVREGIELR
                     LLGKLIAQAGLMPGQAALATPESAAFQNGLAAVASDRGSLASVLEAIQRLTGTLRDRF
                     SPDMMITVGPLLNEVRSGLREAGGNFAPPLAALDHILRFVSTLSGLASENMTRGTGWL
                     FLDLGRRIERAQFVLRAALGPLQQSPIDWDAAMRLALELCDSSITYRWRYLGQLQPAP
                     VLDLVVLDDSNPRAFAFQMRSIASHLRYLAEASGTRVPNPLEPLDDELARAVALFAGE
                     EKSQEEQALALLRRIATGGQERLEELSEAITRAYFSHVPAAQAVGSMAGMAAMAAS"
BASE COUNT          885 a         1991 c         2060 g          819 t
ORIGIN      
        1 tccgctgcca aataggctgc caaacagatc gccaagccgg gcgagcagcg attagcgccc
       61 cacgtcgcgc attcttctct gatcgcaaac gacatgcccc cttccgtccc gcggcatgtt
      121 agaaacacgc cgccattgat ctgaaatcat ttaacagggg cagcagtatg gattggacgg
      181 agcggcggga gcgcttgcgc tcgctcatcg cgggaccgcg ttgcatctat ccgggatcgg
      241 tcttcgacgc gatctcggcg cgcattgccg aagatctcgg ctatgaagcc ggcatgtttg
      301 ccggctcgat cgcttcgttc gcggtgctcg gtgcaccgga tgtggtggtg ctgacgctga
      361 ccgaattcgc acagcaggct taccgcatca atcgcgccgg caaattgccg ctcctggtcg
      421 atgccgacca tggctacggc aatgcgctaa gcgtcaaacg cacagtcgag gagcttgaaa
      481 ccgccggcgt cgctgccatg acgatcgaag acaccgcgct gcccacagct tacggcgcga
      541 gcgggccgaa cctcattccg atcgcggagg gtgtcggcaa gatgaaggcg gcgctggccg
      601 gccggcagga tccgctgctg gcgatcgccg ggcgcaccag cgcgatccag atcaccggcc
      661 tcgacgacgc gatcgcccgc ggcaaggcct accaggaggt cggcgtcgac gcgctgttct
      721 tcgtcggcgt gaagacccgc gccgagctcg atgcgctgtc ggccgccacc aggattccga
      781 tcatcctcgg cggcgccacg cccgagctga ccgacaggga gtatctcgcc agccgccgcg
      841 tgcgcatcgc cctgcagggc caccagccgt tcgccgccgg cgtgaaggcg gtctacgaga
      901 ccctgaaggc gctgcgcgag ggcaccccgc ccggcaagct gcagggcgtg gccgacgccg
      961 acttgatgaa gcgcgtgacg cgcgacgcgg actatgccaa gtggacgaag gggtttctcg
     1021 gcggctgaag gagcggacgt gaggcggcgg gcttttctgg ctacgggcgc gctcgcgctc
     1081 gccaccacgc cgctgcgcgc ccaacgcctg gcgcgcgtcg cctttctcgg cgtgcaggcc
     1141 gaggcgacgc aggatccccg gcagatcgag caattcaagg cggggctggc ggaaaacagg
     1201 ctggtcgacg gccgcaccgt cgccgtcgaa taccactggg ccgacggcag gccggagcgc
     1261 ctgcgggcgc tggcccgggc gctgggcgcg cgcagcgacc tcgatgtcat cgtcacggtg
     1321 ggctcgcagg cggtgcgcga gctgatggcg accggaaccc gcatcccgat cgtcctcgcg
     1381 gtgatcggcg atccggtccg cccgggcttc gtgaagacgc tggcgcggcc gggcggcacg
     1441 atcaccggcc tgtcgatgtc gaactccgag ctcgagggca agcggctgga catcctgcgc
     1501 gccgcggttc cgtcgatcgc cagggtcatg gtgctgctgg atcccggcat ggagggcgaa
     1561 ggcgtcgacg tcgtcgagcg caacgcccga tcgctcggca tcgagttgct gatgacgccg
     1621 gtcgccaacg ccgggcagct cgctcccggc ttcgcctccg ccgccggccg cggcgccgac
     1681 agcctggtga caatggcctc gccgttcctc aacttccagc gcgaccgcat catcgcgctg
     1741 gccaaccagc atcgcctgcc gtcgatctgg gaggtcgaca ccttcacccg cgcgggcggc
     1801 ctgatgtcgt acgggccgag cttcgccgac atgtaccggc gctcggcggg cttcgtcgcc
     1861 aagatcctga agggcgccaa ccccggcgac ctgccggtcg agcagccgac gcgcttcgag
     1921 ctggtggtca acatgcgtac cgcgcggctg ctcggcctca ctttttcgca gtcctttctg
     1981 gcgcgtgctg acgagctgat cgaatgatca agcgacgctt cctcatggcg tccggcctcg
     2041 ccgcggtctc gagccgatcg gtgcgcgccc agcggccacc gctgatcggc tatctcggcg
     2101 gccgctcgct tgccaccgac gcgcacctgc tgcaggcggt gaaggacggg ctgaaggaga
     2161 acggcttcgt cgagggccgc aacgtcaagt tcgagttccg ctgggccgac ggcgacttct
     2221 cgcgactcga ccgtctggcg aaggagctgg tggcgctgaa gcccgatctc ttgatggcgg
     2281 tcggcggcac gccggtgccg atcgcgatgg agcgcgcgcg cgggagcctg gccattccgc
     2341 tcgtcttcac catcggcttc gatccggtga gcctgaagct ggtcgacagc ctggcgcggc
     2401 ccggccacga cgcgaccggc gcgatgcttc tcgccaacgc gctcgagggc aagcggctcg
     2461 acctgctgaa ggacatgcag ccaggcctgc agtcggcggc gctgctggtc aactcgacca
     2521 gcgccatggt gaaccagctc gaggccgacg cgcagcgggc ggcggctgcg ctcggcgtgg
     2581 cgctccattt cgtgaaggtc tcgaccgtcg ccgagttgga cgcggcactg gccgacctcg
     2641 cgccgagcaa gcccggcgcg ctggcggtgt cgatcgacgg cttcctgatc gcccagcgcc
     2701 ggcgcatcat cgactgggcg gcgcgcgagc gggtgccggc gatctacccg aaccgcgaat
     2761 tcaccgaagc aggcggactg gcgagctacg ccgcgcgctg ggccgaggcc taccactggg
     2821 ccggcgtcta cgccggccgc atcctgaggg gcgccaaggc cggcgatctg ccgatccagc
     2881 agccgaccgc ctacgagctg gtcgtcaacc tcaagaccgc ccgcgcccag ggcatcacgc
     2941 tgccggccgc gttcaccgcc cgggcggacg aggtgatcga gtaggcgccg attggaagta
     3001 ggacggaatc agcccgtcgg caccagcgtc accgccacgc tgtaggcgtg gctgccgccg
     3061 cccaggatga cgccgcgcaa agggctcacg tcggagaagt cgcggcccca ggcgacggcg
     3121 acatggtcct cgtgcgcgac caggtcgttg gtcgggtcga gatggaccca gccggcgcgc
     3181 tcgccgcacc acaccgcgac ccaggcgtgg ctggcatcgg cgccgcgcag cgccagctcc
     3241 tcggccgaag gaacggtgcg gatgtagccc gagacatagg ccgcggcgag gccgtgcgcg
     3301 cgcagcgcgg cgatcatcac gtgggcgaag tcctggcaga cgccggcctt gcccgcgaat
     3361 acctcgtgca aggccgtcga gatgtcggtc gcgccgggat ggtattcgaa gtcggccttg
     3421 atgcgcgtcg tgagctcgcg ggccgccgcg aggatcggcc ggccgggcgt gaaggagcgc
     3481 gcgccgtagg ccgccagttc ttcgtcgatc ggaacgagcg gcgagtcgtg gacgaactcg
     3541 ctcgcctcga tcggcttggg gaagccgttg ccgtcgagcg cgcgggcgat ctcctcccac
     3601 gccggcgtct ggtcggccgg cggcggcacg ggaaagcgca cctcgatcgc ggcgcgcacc
     3661 tcgatgtcga agcgccggtg cggctggtcg atgcgcaaca ggtcgatcag gttgccgaaa
     3721 tggtcgacat gctgcgtcgc catcgacggc tcgggctgcg cggtgacgcc gatcgaggtc
     3781 accgtctggc cggggaacga gcgcgcgcgc aggtgggcga tgtggtgcgc gctgtcgacg
     3841 gtgctggcgt agcggtaggt cgggcgatgc gacagcaggt aatgcatcag ctggccgcca
     3901 tcgccgccat ccccgccata gagccgaccg cctgcgccgc cgggacgtgg ctgaaatacg
     3961 cgcgggtgat cgcctcgctc aactcctcga gccgctcctg cccgcccgtc gcgatccggc
     4021 gcagcaacgc cagcgcctgc tcctcttgcg atttctcctc gccagcgaac agcgcgaccg
     4081 cgcgggcaag ctcgtcgtcg agcggttcca acgggttcgg cacccgcgtc cccgaggcct
     4141 cggctagata gcggaggtgg ctcgcgatcg agcgcatctg gaaggcgaag gcgcgcgggt
     4201 tgctgtcgtc gagcacgacg aggtcgagca cgggcgcggg ctggagctgg ccgagatagc
     4261 gccagcggta ggtgatcgag ctgtcgcaca gctcgagcgc cagccgcatc gccgcgtccc
     4321 agtcgatcgg cgactgctgc aacggcccga gcgccgcgcg cagcacgaac tgcgcccgct
     4381 cgatgcgccg gccgagatcg aggaacagcc agccggtgcc gcgcgtcatg ttctcgctgg
     4441 cgaggcccga cagcgtcgag acgaagcgga ggatatggtc gagcgcggcg agcggcgggg
     4501 cgaaattgcc gccggcctcg cgcaggccgc tgcgcacctc gttcagcagc gggccgacgg
     4561 tgatcatcat gtcgggcgag aagcggtcgc gcagcgtgcc ggtcaggcgc tggatcgcct
     4621 cgagcaccga ggcgaggcta ccgcggtccg atgcgacggc ggcgaggccg ttctggaagg
     4681 ccgcgctctc gggcgtcgcc agcgccgcct ggcccggcat caggccggcc tgggcgatca
     4741 gcttgccgag caggcggagc tcaatgccct cgcgcacgcc gatcacgccc tgcgccacgc
     4801 gggtgacggt gaggcgcagc aggcgggcat tgtcgtcgag ccgctcgacg tagcggccga
     4861 gccagtagag gttgtcggcg acgcggctct gcaggtcggc gccgccgcga tcgaccgcga
     4921 gcgggtggaa gcggcggctg gccgggatct gcacgtcggc cgcgtcctcg gccagcaccc
     4981 agacgtcctt gagcgtgccg ctgagccgcc cgagctggcc gagcgcggtg tttcccgccg
     5041 gctcgcgcgc caggcctccc ggcagcacgc gccagctcgc accattcgag accaggaagc
     5101 agcgcagcac gacggccgac ggcgccaatt gcgcgccgtc ccatttcgga ttgagcgacg
     5161 gcgagacggg agactgcgcc acgaagtggc cggggttggc cgagaggcgc tgccgcaggc
     5221 gatggcgcgc ctcgccctcg agcatgccga cgacgatcgg ctcgcggtcc gggccgaggc
     5281 agggccgcac gatcatcgca tcgagattcg ccaggacgta gtccttcgcc ggcggctcgc
     5341 ccaaccacca cacgtcgagc gagggcagct tcagcggctg gccgagcagg cgctggctga
     5401 tgccataaag aaacggcatc agcgccggcg tctcgaccag gcccgagccg agcgcgttgg
     5461 ccacggcgat cttggccgtc cgcgtcgcct cgacgaggcc ggtgacgccg agcgcgctgt
     5521 cggcgcgcag ctcgagcgga tcggcgaacg acgaatcgag ccggcgcagc agcatgtgca
     5581 cgggccgaag gccggccagc gtcttgatca gcacctcgcc gtcgcgtgcg accaggtcgc
     5641 cgccctcgac cagcgacacg cctaaagcgc gcgacagata gacgtgctcg aagtaggtcg
     5701 ccgacagcga gcccggcgtc agcacggcta tgttcgggtt cgacatgccg tgcgg
//
LOCUS       DASRWS010000510         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_215463, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000510 DASRWS010000000
VERSION     DASRWS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215463"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFF72_09580"
     CDS             complement(<1..338)
                     /locus_tag="VFF72_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HZV93457.1"
                     /translation="MTATSQTQAVPEEIQARVSGSSFYAAMLLMPKAERQAMFAVYRF
                     CRLVDDVADDGTRPRPERAKELARWRADIAALYAGKPSGQAAFLAPIVRDFALREADF
                     LTVIDGMDMDV"
     gene            complement(335..1222)
                     /gene="hpnC"
                     /locus_tag="VFF72_09585"
     CDS             complement(335..1222)
                     /gene="hpnC"
                     /locus_tag="VFF72_09585"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZV93458.1"
                     /translation="MSDAASFASGKGHRDENFPVASWLIKPALRAPVLAFYRFARAAD
                     DVADHATASAEEKLAALARMEAGLGGEAGASAEGDALRAALRDHGVTDQHARDLLEAF
                     RRDVTKRRYTDWADLMDYCRYSAAPVGRFVLDVHGESRATWPANDALCSALQVINHLQ
                     DCAKDYRNLDRVYIPLDALAGQGIGVEALGEKRASAPLRAVLVALAQRTNAFLDQSRV
                     FAGQIADRRLALEVGVIQTLAESLTNRLMRRDPLSERVHHRPVEMIGLALRGGLGVAA
                     GRLRRGISGKQPDLRGSQG"
     gene            complement(1219..>2028)
                     /locus_tag="VFF72_09590"
     CDS             complement(1219..>2028)
                     /locus_tag="VFF72_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZV93459.1"
                     /translation="QGVQHATNAGAPPDYILLTDADIGHASDNLRALVSRAEQEGRVL
                     VSLMAELSCTSWVERFLIPAFVFFFQMLYPFAWVAKARGIAAAAGGCVLVRREALERA
                     GGIASIRAEIIDDCALARRLKAEGPIWLGLTRRAKSLRPYGGLAEIGRMISRSAYAQL
                     GYSPLLLLGTVAGMLLVYGVPPLLALFAEGPARWLGLAAWLAMALAFQPMLRFYRVSP
                     LWGVALPAIAAAYALFTVDSALQVWRGRGGMWKGRAQAMTGDMMDGTTGGT"
BASE COUNT          370 a          706 c          663 g          289 t
ORIGIN      
        1 acatccatat ccatgccgtc gatgacggtg aggaagtccg cttcccgcag cgcgaagtcg
       61 cgcacgatcg gagcaaggaa cgccgcctgt cccgacggct tgccggcata gagcgctgcg
      121 atgtcggcac gccagcgagc aagctccttt gcccgctccg gacgtggacg tgttccatca
      181 tcggcgacgt cgtcgaccag ccggcagaag cgatagaccg cgaacatggc ctgccgttcc
      241 gccttcggca tcagaagcat ggcggcatag aacgagctgc cggagacccg cgcctggatc
      301 tcctctggca ccgcctgcgt ctgagaagtg gcggtcatcc ttgactgcca cgcagatcgg
      361 gttgcttgcc gctaatgccc cgtcgcagcc ggccggccgc gacgccaagg ccgccgcgca
      421 gggcaaggcc gatcatctcc acgggccgat gatggacgcg ctccgacaag ggatcgcgac
      481 gcatcaaccg gttggtcagg ctctcggcca gggtctggat gacaccgact tccagcgcga
      541 ggcgccggtc cgcgatctgg cccgcgaaga cccgcgactg atcgagaaat gcgttggtgc
      601 gctgcgccag ggccacgagc accgcgcgca gtggtgccga cgcgcgcttt tcgcccaagg
      661 cctcgacgcc gatgccctga ccggcaagag cgtcgagcgg gatatagacg cgatcgagat
      721 tgcgataatc cttggcgcaa tcctgcaggt ggttgatcac ctgcaaggcg ctgcagagtg
      781 catcattggc gggccacgtc gcccgcgatt cgccgtgcac atcgagcacg aagcgaccga
      841 cgggcgccgc cgagtagcga cagtagtcca tcaggtcggc ccagtcggtg tagcgacgct
      901 tggtgacgtc gcggcggaag gcttcgagga ggtcgcgggc gtgctggtcc gtgacgccat
      961 ggtcgcgcag cgccgcgcgc aatgcgtcgc cctcggcgct ggctccggct tcaccaccga
     1021 gccccgcctc catgcgcgca agggccgcga gcttttcctc ggcactggcc gtggcgtggt
     1081 cggccacgtc gtcggcagcg cgggcgaagc gatagaaggc cagcaccggc gcgcgcagcg
     1141 ccggcttgat cagccacgac gcgacgggaa agttctcgtc gcgatggccc ttgcctgaag
     1201 caaacgaggc cgcgtcgctc atgtgccgcc tgttgtgccg tccatcatgt cgcccgtcat
     1261 cgcctgggcc cgtcccttcc acatgccacc ccgaccgcgc cacacctgaa gcgcggagtc
     1321 taccgtaaac aacgcatagg cagccgcgat ggccggcagc gctactcccc acaagggcga
     1381 gacacgatag aagcggagca taggctggaa cgccagtgcc atcgcaagcc aggcggcgag
     1441 ccccagccaa cgggccggac cttccgcaaa caacgccagg agcggcggca ccccgtagac
     1501 cagcagcatc cccgcaaccg tgccgagcag cagcagcggt gaatagccca gctgggcata
     1561 ggccgagcgc gagatcatgc ggccgatctc ggcaaggcca ccataaggcc gcaagctttt
     1621 ggcgcgccgc gtgaggccga gccagatcgg cccctctgcc ttcagtcgcc gggccagcgc
     1681 gcagtcgtcg atgatctccg cacggatgga cgcaatgccg ccggcccgtt ccagcgcctc
     1741 gcgccgcacc agcacgcacc caccggccgc cgccgcgatg cccctcgcct tcgcgaccca
     1801 ggcaaaggga tagagcatct ggaaaaagaa cacgaacgcc gggatcagga aacgctcaac
     1861 ccagcttgtg caggagagct ctgccatcag cgagaccagc acgcgtcctt cctgctcggc
     1921 gcgcgaaacg agcgcgcgca gattgtcgga ggcgtgtccg atgtcggcgt cggtcaacag
     1981 gatgtagtcc ggaggcgcac cggcgttcgt cgcgtgttgc acgccctg
//
LOCUS       DASRWS010000511         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4225770, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000511 DASRWS010000000
VERSION     DASRWS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225770"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VFF72_09595"
     CDS             <1..173
                     /locus_tag="VFF72_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HZV93460.1"
                     /translation="PGSGNLGVLQAGALEESNVDLTGELVNMITAQRDYQANAQTIKT
                     EDQIMQTIVNLR"
     gene            365..1090
                     /gene="flgF"
                     /locus_tag="VFF72_09600"
     CDS             365..1090
                     /gene="flgF"
                     /locus_tag="VFF72_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HZV93461.1"
                     /translation="MDRLIYLSMAGAKAAMQRQDTLANNLANVSTTGFRAELSAFRAV
                     PVQGSGASTRVYALESTPGYSDAAGVVSGTGRSLDVAMKDNAWLAVQGLDGTEAYTRN
                     GSLDLSADGTLTTTSGLTVLGDGGPIQVPPNTSVSIAADGTVSATDASGRSNAIGKLK
                     LVTPDAPLTRGTDGLFRAADGELSADPAARVQIGALEGSNVSAVETMVEMIAASRQFE
                     AQMRSLSNAESNDKSAQQLLSAS"
     gene            1090..2415
                     /locus_tag="VFF72_09605"
     CDS             1090..2415
                     /locus_tag="VFF72_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZV93462.1"
                     /translation="MATAASPAPGLADAIPGSELQALLFPIGADEFVHRYWGREPLYL
                     RGDAAKVRGFFDGEAFREALAQAGPPGADVLRASFDAKTDDGRSASALAGEQVSSVFR
                     ASLEQAVPLFRAGATLCLSQVERRVPRLAQFLAAIKRQLGYPGRASFSAYLSPPGSGF
                     NWHFDARIASTLQIEGRKRWRYTAHAATPWPRGNGSLDAAARPRYTDPGCGPSDELVG
                     LGDGPVGEVTLEPGDLLVLPAGVWHEACGGDEGSLALNLSFTPLPYTLLAQRLLDALL
                     MDEPGWRSMNPALPAGPAGEIDAAGVAAISAQLARAASALQALSGDSAQVLRLWQSLV
                     GGEMPPATPVVAGPAPVLPMERLRLRAEAGVHAMLAEQGAQLLVATGEGRRLELSGDA
                     VAFVRRLLSTPEFTAASCLEWTGGGAAFAWDDVAGMLGQLRSQGLIESV"
BASE COUNT          339 a          934 c          879 g          345 t
ORIGIN      
        1 tcccgggttc gggcaatctc ggcgttctgc aggccggcgc gctcgaggag tcgaacgtcg
       61 acctgaccgg cgagctggtc aacatgatca ccgcgcagcg cgactaccag gccaacgcgc
      121 agacgatcaa gaccgaagac cagatcatgc agacgatcgt caacctgcgt tgatgagacc
      181 cccacgctca cgggtacgct cgctgccccc cgagggggcg cggccggcct tgggacggcc
      241 cggcggccgg ccggtgagac ccccacgctc acgagtacgc tcgctgcccc ccgagggggc
      301 gcggccgatg ctccttcgac actcacgttc gccgacgccg tctttcgact gatcggagcc
      361 acgcatggat cgactcatct acctgtcgat ggcaggcgcc aaggcggcga tgcagcggca
      421 ggacacgctc gccaacaacc tcgcgaacgt ctcgaccacc ggttttcggg ccgagctctc
      481 ggcgtttcgc gccgtcccgg tgcagggcag cggtgccagc acgcgcgtct acgcgctcga
      541 gtcgacgccg ggctacagcg atgccgcggg cgtcgtcagc ggcaccggac gcagcctcga
      601 cgtggcgatg aaggacaacg cctggctcgc cgtgcaggga ctcgacggca ccgaggcata
      661 cacccgcaac gggtcgctcg acctgagcgc cgacggcacc ctcacgacca cgtcgggcct
      721 caccgtgctc ggcgacggcg gaccgatcca ggtgccgccg aacaccagcg tctccatcgc
      781 cgccgacggc accgtgagcg cgaccgacgc gtcgggccgc agcaacgcga tcggcaagct
      841 gaagctggtc acacccgacg cgcccctgac ccgcggcacc gatggcctct ttcgtgccgc
      901 cgacggcgag ctttcggccg acccggcggc gcgggtccag atcggcgcgc tcgagggctc
      961 gaacgtcagc gcggtcgaga cgatggtcga gatgatcgcc gcgtcgcgcc agttcgaagc
     1021 gcagatgagg agcctgtcga acgcggagag caacgacaag agcgcgcagc agctcctgtc
     1081 ggcaagttga tggccacggc cgcgtcgccg gcgccgggcc tggccgacgc gattcccggc
     1141 tcggagttgc aagcgctgct gtttccgatc ggtgccgacg agttcgtgca tcgctactgg
     1201 gggcgcgagc ccctctacct gcgcggcgac gccgccaagg ttcgcggctt tttcgacggc
     1261 gaggcgttcc gcgaggccct ggcgcaggcc gggccgcccg gcgcggacgt gttgcgggcc
     1321 agcttcgatg ccaagaccga cgacggtcgc tcggcgtcgg cgctcgccgg cgagcaggtc
     1381 tccagcgtct ttcgcgccag cctggagcag gccgttcccc tctttcgcgc cggggcgacg
     1441 ctgtgcctca gccaggtcga acgacgcgtg ccgcgccttg cgcaatttct ggcggcgatc
     1501 aagcgccagt tgggctatcc cggccgcgcc agcttcagcg cctacctctc gcctcccggc
     1561 tcgggcttca actggcattt cgatgcgcgc atcgccagca ccctgcagat cgaaggccgc
     1621 aagcgctggc gctacaccgc gcacgcggcg acgccctggc cgcggggcaa cggcagcctg
     1681 gacgccgccg cccggccgcg ctataccgac ccgggctgcg gcccgtccga cgagctcgtc
     1741 ggcctcggcg acggcccggt cggcgaggtc acgctcgagc cgggcgacct gctggtgctg
     1801 ccggccggcg tgtggcacga ggcctgcggt ggcgacgaag ggtcgctcgc gctcaacctc
     1861 tcgttcacgc cgctgcccta cacgctgctc gcgcagcgcc tgctcgatgc gctgctgatg
     1921 gacgagcccg gctggcgcag catgaacccg gcgctgcccg cggggccggc cggcgagatc
     1981 gacgccgccg gcgtcgccgc gatctcggcg cagctggcgc gcgccgcgtc ggcattgcag
     2041 gcgctgagcg gcgacagcgc gcaggtgctg cgcctgtggc agtcgctcgt cggcggcgaa
     2101 atgccgcccg ccacgccggt cgtggccggg ccggctccgg tcctgccgat ggagcgcctg
     2161 cgcctgcgtg ccgaggccgg cgtgcacgcg atgctcgccg agcagggcgc gcagcttctc
     2221 gtcgccaccg gcgaaggccg ccgcctcgag ctgagcggcg acgccgtcgc cttcgtgcgc
     2281 cggctgctgt cgacgcccga gttcaccgcc gcctcgtgcc tcgagtggac tggcggcggt
     2341 gcggccttcg cctgggacga cgtcgccggg atgctcgggc agctgcgcag ccaggggctg
     2401 atcgagtcgg tctagtcgtg ccgcgcagcg gccgtcgcac gccgcggcgc gctgcgccgc
     2461 aaaatccccc tctcgcgccg tcgcggtccc gcgccct
//
LOCUS       DASRWS010000512         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3654787, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000512 DASRWS010000000
VERSION     DASRWS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654787"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFF72_09610"
     CDS             <1..384
                     /locus_tag="VFF72_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZV93463.1"
                     /translation="RTSFVRLVAGAVLGFAAIAAGAAELPFNQVAFDKAVAAGQPVIV
                     DVQASWCPTCKAQKPIVEALMREPKMRKVTLFSADFDTEHALKKQLRVVQQSTFVVFK
                     GGKEVGRSTGETDRGRIESLFDKAL"
     gene            419..1147
                     /locus_tag="VFF72_09615"
     CDS             419..1147
                     /locus_tag="VFF72_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HZV93464.1"
                     /translation="MEIAPATFALAFGAGALSTLSPCVVPLLPILVASALAQHRLGLW
                     ALAAGLALSFTAIGLFVATIGVSIGIDSERLHRLAGALLLAFGAVMASGRLQAAFTTG
                     VSRLSGAGSALLGRVGGQGATGQFAVGLLLGIAWTPCVGPTLGAASTLAAQGRQLGSV
                     AWLMLVFGLGAATPLVALGTLSQRLAGRLGGRWLAAADVARRALGLCLVVVGLVIVVG
                     ADKWIETWLVDHSPAALTELTTRF"
     gene            complement(1215..2810)
                     /locus_tag="VFF72_09620"
     CDS             complement(1215..2810)
                     /locus_tag="VFF72_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HZV93465.1"
                     /translation="MDDPILLARHGDTLCRLLPAMANRHGLVTGATGTGKTVTLQAMA
                     QAFSERGVPVFMADVKGDLAGITQPASPSPKMAALLKERGIEMPAPAACPATLWDVYA
                     DDKSALGHPVRATVSDMGPLLLGRMLDLNEIQQGVLQLVFKIADDQGLLLLDLKDLRA
                     MLQHVGDNASAFTTGYGNISAASIGAIQRGLMQIAAQGGDRFFGEPMLAIADFMQTVP
                     AAGGGRAQGVVNILAADKLLNSPRLYATFLLWMLSELFEQLPEIGDPDEPKLVFFFDE
                     AHLLFDQAPKVLLERIELVVRLVRSKGVGVYFVTQNPLDVPDSILGQLGNRVQHALRA
                     FTPRDQKAVKAAASTMRANPGLDIETAITELATGEALVSLLDEKGRPGVTERVFVIPP
                     ASRIGTISDAERQALIAGSLVAGVYEKTIDRESAYEKLKGRAVSTGAAGGAATGATAA
                     TESPQGAASPAGTASAPAPSGDGLLGGLKNAIFGTTGPRGGRHEGLAESAARSAMRSI
                     GSSVGREIIRGVLGSLSGGSRRR"
     gene            2934..>3299
                     /locus_tag="VFF72_09625"
     CDS             2934..>3299
                     /locus_tag="VFF72_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZV93466.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKVWTRLIREIVVAARQGGGDLA
                     MNPRLRLAVEKAKAANMPADTIRKNVDKATGNLEGVSYEEIRYEGYGIGGAALLVDCV
                     TDNRVRTVAEVRHAFSKYGG"
BASE COUNT          455 a         1129 c         1198 g          517 t
ORIGIN      
        1 aggacatcgt tcgtccggct cgttgccggt gcggttctcg gcttcgcggc gatcgccgcc
       61 ggcgccgcgg agctaccctt caaccaggtc gcgttcgaca aggcggtggc cgccggccag
      121 ccggtcatcg tcgacgtgca ggcgtcgtgg tgcccgacct gcaaggcgca aaagccgatc
      181 gtcgaggcgt tgatgcgcga gcccaagatg aggaaggtga ctctcttctc ggccgacttc
      241 gacaccgagc acgcgttgaa gaagcagctg cgcgtcgtcc agcagagcac tttcgtcgtc
      301 ttcaagggcg gcaaggaggt cggccgctcg accggcgaga ccgaccgggg tcgcatcgag
      361 tcgctgttcg acaaggcgct gtgaagcgcg gccgccgggt ggcccggcgg cgccgatgat
      421 ggagatcgcc cccgcgacct tcgccctggc cttcggcgcg ggcgcactgt cgacgctgtc
      481 gccctgcgtc gtgccgctgc tgccgatcct ggtcgccagc gcgctggccc agcaccggct
      541 gggactctgg gcgctcgcgg ccgggctggc gctgtcgttc acggcgatcg gtcttttcgt
      601 ggcgacgatc ggcgtgtcga tcggcatcga ctcggagcgc ctgcatcgcc tcgccggcgc
      661 gctgctgctc gccttcggcg ccgtcatggc gtcgggccga ctgcaggcgg cattcacgac
      721 cggggtgtcg cgcctgtccg gcgccggcag tgcgctgctc ggccgcgtcg gcggacaggg
      781 cgcgaccgga cagttcgcgg tcggcctgct gctcggcatc gcctggacgc cctgtgtcgg
      841 accgacgctc ggtgctgcga gcacgctcgc cgcgcaggga cgtcagctcg gcagcgtcgc
      901 ctggctgatg cttgtcttcg gcctcggcgc ggcgacgccg ctggtcgcgc tgggcacgct
      961 ctcgcaacgc ctggccggcc gcctgggcgg tcgttggctg gcggcggccg atgtcgcgcg
     1021 acgcgcgctc gggctctgcc tggtggtcgt gggactggtc atcgtcgtcg gcgccgacaa
     1081 gtggatcgag acctggcttg tcgaccattc gcccgccgcg ctgacggagt tgacgacacg
     1141 cttctgaaag ccgccacgcc gcagggccgg gcgtgcgacg cgacggcagc ctcgcgcggc
     1201 gccgggtcgc gctgtcagcg ccgtcgcgag ccgcccgaca ggctgccgag aacgccgcgg
     1261 atgatctcgc ggccgacgct cgagccgatc gatcgcatcg ccgagcgggc tgccgattcc
     1321 gcaagacctt cgtgccggcc gccgcgcggc ccggtcgtgc cgaagatcgc attcttgagg
     1381 ccgccgagca ggccatcgcc ggacggtgcc ggcgcgctcg ccgtgccggc cggcgaggcg
     1441 gcgccttgcg gcgattcggt cgctgcagta gcgccggtcg ccgcgccgcc agccgcgccg
     1501 gtcgagacgg cgcgaccctt cagcttctcg tacgccgatt cgcgatcgat cgtcttctcg
     1561 tacacgccgg cgaccagcga gccggcgatc agcgcctggc gctcggcgtc gctgatggtg
     1621 ccgatgcggc tcgccggcgg gatgacgaag acgcgttcgg tgacgccggg ccgacctttc
     1681 tcgtcgagga ggctcaccag cgcctcgccg gtggccagct cggtgatcgc agtctcgatg
     1741 tcgaggccgg ggtttgcgcg catcgtgctc gccgccgcct tgaccgcctt ctggtcgcgt
     1801 ggcgtgaagg cgcgaagcgc gtgctggacg cggttgccga gctggccgag gatggaatcg
     1861 ggaacgtcga gcgggttctg ggtgacgaaa tagacgccga cgcccttgct tcgcaccagc
     1921 cgcacgacca gttcgatccg ttcgagcagc accttgggcg cctggtcgaa cagcaggtgt
     1981 gcttcgtcga agaagaagac cagcttcggc tcgtcgggat cgccgatctc gggcaactgc
     2041 tcgaagagct cggagagcat ccagagcagg aacgtcgcgt agaggcgcgg gctgttcagc
     2101 agcttgtcgg cggccaggat gttgacgacg ccttgcgccc gtccgccacc ggccgcgggc
     2161 acggtctgca tgaagtcggc gatggccagc atcggctcgc cgaagaaacg atcgccgccc
     2221 tgtgccgcga tctgcatcag gccgcgctgg atcgcaccga tgctggcggc gctgatgttg
     2281 ccgtacccgg tcgtgaacgc cgacgcgttg tcgccgacgt gctgcagcat ggcccgcagg
     2341 tccttcaggt cgagcagcag caggccctgg tcgtcggcga tcttgaagac gagctgaagc
     2401 acgccttgct ggatctcgtt gaggtcgagc atgcggccga gcaggagcgg gcccatgtcg
     2461 ctgacggtgg cgcgcaccgg gtgccccagc gccgacttgt cgtcggcgta gacgtcccag
     2521 agcgttgccg ggcacgctgc cggcgccggc atctcgatgc cgcgctcctt cagcagcgcc
     2581 gccatcttcg gcgacgggct cgccggctgc gtgatgccgg cgaggtcgcc cttcacgtcg
     2641 gccatgaaga ccggcacgcc gcgctcgctg aaggcctgcg ccatcgcctg cagggtgacg
     2701 gtcttgccgg tgccggtcgc gccagtgacc aggccgtgcc ggttcgccat cgccggcaag
     2761 agccggcaca gcgtgtcgcc gtgtcgggcg agaagaatgg gatcgtccat gcgtgaccgg
     2821 tcgcgagagg gatggcgacc cagtctatcc ggcgaagccg cgtcggcgcc gggagcccgc
     2881 gtctagaatc ttcgccgtcg aagggaagtc gccgcgatgg cgcatgcaca cgtatggccg
     2941 gtcattcgaa gtgggcgaac atccagcacc gcaagggccg ccaggacgag aagcgcggca
     3001 aggtctggac ccggctgatc cgcgagatcg tcgtcgccgc gcgccagggc gggggcgacc
     3061 tggcgatgaa cccgcgtctg cgcctggccg tcgagaaagc caaggccgcc aacatgccgg
     3121 ccgacacgat ccgcaagaac gtcgacaagg ccaccggcaa cctcgagggc gtcagctacg
     3181 aggagatccg ctacgagggc tacggcatcg gcggcgcggc gttgctggtc gattgcgtca
     3241 ccgacaaccg ggtccgcacc gtcgccgagg ttcgccatgc cttcagcaag tacggcggc
//
LOCUS       DASRWS010000513         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_216552, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000513 DASRWS010000000
VERSION     DASRWS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216552"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFF72_09630"
     CDS             complement(<1..370)
                     /locus_tag="VFF72_09630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV93467.1"
                     /translation="MDIEIRRATAEDFPAIAELDGASFRFHYSAEELTDAVLDVAPEG
                     FVLASDGDTIVAISAELPLQMTVPGGAELDVMGLTWVSVELTYRRRGVLRSVIESQLR
                     AHAEAGLAATVLTASEGGIYG"
     gene            complement(405..>2088)
                     /locus_tag="VFF72_09635"
     CDS             complement(405..>2088)
                     /locus_tag="VFF72_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93468.1"
                     /translation="ARARGDNNAADLLQRVHPMPGAGIDFTELGHILLLAIAVYAGAS
                     ALSWLQGWLLNGVVLNSVRRLRKDVEDKINRLPLPYFDQQPRGELLSRVTNDIDNIQM
                     SLQQTMSQLLTSLLTVVGVVTMMFVISPVLALIALVTIPVSMVVTKTIAKRSQSKFIR
                     QWKNTGQLNGQIEEAFTGHELVKVFGRHEEIEGEFDRKNEELYQAAFGAQFISGVIMP
                     AMMFIGNLNYVAIAVVGGLRVASGSMSLGDVQAFIQYSRQFTQPLTQVASMANLLQSG
                     VASAERVFDLLDAEEQLPDPAQPKKPIEKRGRVEFEHISFSYSADAPLIEDLSLVADP
                     GHTIAIVGPTGAGKTTLVNLIMRFYELDSGRITLDGVDITAMRRDQLRGEIGMVLQDT
                     WLFGGTIRDNIAYGRPGASEEEILAAARAAFVDRFVQALPDGYDTVIDEESSNLSTGQ
                     KQLMTIARAFLAEPSLLILDEATSSVDTRTEVLVQHAMAALRANRTSFVIAHRLSTIR
                     DADVILVMESGRIVEQGNHEALLAARGAYYDLYNAQFTQAPDETEGAPVPVG"
BASE COUNT          310 a          702 c          721 g          355 t
ORIGIN      
        1 gcccgtagat gccgccctcg ctggccgtga gcacggtggc cgccagcccg gcctcggcgt
       61 gcgcgcgcag ctggctctcg atcaccgagc gcagtacgcc tcgccgccgg taggtgagct
      121 cgaccgagac ccaggtcaac cccatgacgt ccagctcggc cccacccggg accgtcatct
      181 gcaacgggag ctcggcgctg atggccacga tcgtgtcgcc gtcgctcgcg aggacgaagc
      241 cttcgggggc gacgtccaac acggcgtcgg tcagctcctc ggcgctgtag tggaacctga
      301 agctggcgcc gtcgagttcc gcaatcgctg ggaagtcctc cgccgtcgct cgccggatct
      361 cgatgtccac ggctaccgag tctgccggtc gggccaatcg gatatcagcc caccggcacc
      421 ggcgcgccct cggtctcgtc cggcgcctgg gtgaactggg cgttgtagag gtcgtagtac
      481 gcgccgcgcg cggcgaggag agcttcgtgg ttgccctgct cgacgatgcg gccgctctcc
      541 atcaccagga tcacgtcggc gtcgcggatc gtggagagcc ggtgggcgat gacgaaactg
      601 gtgcggttgg cgcgcagcgc ggccatcgcg tgctgcacca acacctcggt gcgggtgtcc
      661 accgagctgg tcgcctcgtc gaggatcagc agcgacggct cggcgaggaa cgcgcgcgcg
      721 atcgtcatca gctgcttctg cccggtggac aggttgctgc tctcctcgtc gatcacggtg
      781 tcgtagccat ctggcagggc ctggacgaac cggtcgacga aggcggcccg cgccgcggcg
      841 aggatctcct cctcgctcgc gcccggccgc ccgtacgcga tgttgtcgcg gatggtgccg
      901 ccgaacagcc aggtgtcctg caggaccatg ccgatctcgc cgcgcagctg gtcccggcgc
      961 atcgccgtga tgtcgacgcc gtcgagcgtg atccggccgc tgtccagctc gtagaagcgc
     1021 atgatcaggt tgaccagcgt ggtcttgccg gcgccggtgg gaccgacgat cgcaatcgtg
     1081 tgaccggggt cggccacgag cgagaggtcc tcgatgagcg gtgcgtcggc cgagtagctg
     1141 aacgagatgt gctcgaactc gacgcggccg cgcttctcga tcggcttctt gggttgagcc
     1201 gggtccggca gctgctcctc ggcgtcgagc aggtcgaaca cccgctcggc cgaggcgaca
     1261 ccggactgga gcaggttggc catggacgcg acctgggtca gcggctgggt gaactggcgc
     1321 gagtactgga tgaacgcctg gacgtcgccg aggctcatgc tgcccgaggc cacccgcagc
     1381 ccgccgacga ccgcgatcgc gacgtagttc aggttcccga tgaacatcat cgccggcatg
     1441 atcacgccgc tgatgaactg cgccccgaac gcggcctggt acagctcctc gttcttgcgg
     1501 tcgaactcgc cctcgatctc ctcgtgccgc ccgaagacct tgaccagttc gtgcccggtg
     1561 aacgcctcct cgatctggcc gttgagctgg ccggtgttct tccactgccg gatgaacttg
     1621 ctctgcgagc gcttggcgat cgtcttcgtg accaccatcg agaccgggat cgtgacgagc
     1681 gcgatgagcg ccagcaccgg cgagatcacg aacatcatcg tgaccacgcc gacgacggtg
     1741 agcagcgagg tcagcagctg gctcatcgtc tgctgcaggc tcatctggat gttgtcgatg
     1801 tcgttggtga cgcggctgag cagttcaccg cgcggctgct gatcgaagta cggcagcggc
     1861 aggcggttga tcttgtcctc gacgtctttg cgcaggcgcc gcacgctgtt cagcacgacg
     1921 ccgttgagca gccagccctg cagccacgag agcgcgctgg cgccggcata gacggcgatg
     1981 gccagcagca ggatgtggcc gagctcggtg aagtcgatcc ccgcgccggg catcgggtgg
     2041 acccgctgca gcaggtcggc cgcgttgttg tcgccgcggg cccgggcc
//
LOCUS       DASRWS010000514         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3400340, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000514 DASRWS010000000
VERSION     DASRWS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3400340"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /gene="smc"
                     /locus_tag="VFF72_09640"
     CDS             complement(<1..2003)
                     /gene="smc"
                     /locus_tag="VFF72_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZV93469.1"
                     /translation="MRLNSIKLSGFKSFVEPTQFQLPGQLVGVVGPNGCGKSNIIDAV
                     RWVLGESKASELRGESMQDVIFNGSSLRKPAGRASVELVFDNVDGRAGGPWSRFSEIA
                     VKRVLTRDGTSSYHINNQAVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESKPEEL
                     RLFLEEAAGVSKYKERRRETENRLKDTRENLTRVEDILRELGQNLDKLQGQAEVAGRY
                     RALQQDAQTRQHQLWFLKHRDASREEERVRQGLVAASAALESKLAELRHAEAELEQIR
                     QSHYAANDELHAAQGALGEAALEVSRIEERIRYVAQGRDRVARQRGDLKVQHESWLAR
                     ESEARGGIDRAKAAIEEGEAAASNLAGRVATQSRALEEAAAAWRTAQDVSATQREVVG
                     AIAQQIQVAAAETRGIDERLAQARGRRERLVGEESSLAPVESALLAAAQERSAAAAHA
                     HAAIESEMAQWQAKLPALEESRRTMQAEANEQSRRHADLGARLGALRALQEKVQVEDK
                     LKPWLARHGLESLHGLWQRVRIEPGWEAALEAALRERLGALEIGRLDTVRAFAADAPP
                     ARLAFFTTPGAPLAPSHRTLPRLAELLHLGDAGLSALLGDWLEGVYTAADLDAALASR
                     SSLTHGEVIMTREGHAVSQFAVSFYAPDSQQAGLLARAQEIENL"
     gene            2135..2581
                     /gene="queF"
                     /locus_tag="VFF72_09645"
     CDS             2135..2581
                     /gene="queF"
                     /locus_tag="VFF72_09645"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZV93470.1"
                     /translation="MAKTSRNALSVSTRRPPATPPSAPQKHLDVFANPAPGRDYLIEF
                     EIPEFTCLCPLTGQPDFARFTIEVIADRLCIELKSLKLYLWSFRDVGAFHEKVTNAIL
                     DDIVSATKPRFARIRAHWNVRGGIVTNVAAEYRAKGWKAKSPALLG"
     gene            complement(2663..>3131)
                     /locus_tag="VFF72_09650"
     CDS             complement(2663..>3131)
                     /locus_tag="VFF72_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93471.1"
                     /translation="AVWPASRLAEWPELEKKSQEAFDRSFGRSAPKEARALGAKMAAR
                     AVFGWAGLGEKLLAAQAGIDDHTLELAKLGVIRHLDRAPVASTVEFRLVDAQADKLML
                     GWIRTATEELLDVVGLPRAAIAEIESSAPDWASLREDVGGGLFVDYRRSLAVA"
BASE COUNT          462 a         1122 c         1035 g          512 t
ORIGIN      
        1 agattctcga tttcctgggc ccgggcgagc aggcctgcct gttgcgagtc gggcgcgtag
       61 aagctcaccg cgaactggct cacggcatgg ccctcacgcg tcatgatgac ttcgccatgc
      121 gtgaggctcg accgtgaagc cagcgcggcg tcgagatcgg ccgccgtgta gacgccttcg
      181 agccagtcgc cgagcagggc cgacaggccc gcatcgccca gatgcagcaa ctcggcaagc
      241 cgcggcaggg tgcgatgcga aggcgccagc ggcgcgcccg gcgtcgtgaa gaaggccagg
      301 cgcgccggcg gcgcgtcggc ggcgaaggcg cgaaccgtgt cgagccggcc gatctcgagg
      361 gcgccgagcc gctcgcgcag cgcggcctcc agcgcggcct cccagccagg ctcgatgcga
      421 acccgctgcc acagaccgtg cagcgattcc aggccatgcc gggccagcca cggcttgagc
      481 ttgtcctcga cctgcacctt ctcctgaagg gcgcgcagcg ctccgaggcg agcaccgaga
      541 tcggcatggc gccgcgattg ctcgttcgcc tcggcctgca tcgtccggcg tgactcctcg
      601 agcgccggaa gcttcgcctg ccactgcgcc atctcggatt cgatcgccgc atgggcgtgt
      661 gccgccgcgg ccgatcgttc ctgcgcggcg gcgagcagcg ccgactcgac cggcgcgagg
      721 ctgctttcct cgccgacgag gcgctcgcgt cggccccggg cctgcgcgag gcgctcgtcg
      781 atgccacgcg tctcggccgc ggccacctgg atctgctggg cgatcgcgcc caccacctcg
      841 cgctgggtcg cactgacatc ttgcgcggtg cgccaggccg ccgccgcttc ctccagcgcc
      901 cgtgactggg tggcgacgcg gccggcgagg ttgctggccg ccgcctcgcc ttcttcgatc
      961 gcggccttcg cacgatcgat gccgccgcgc gcttcgctct cgcgcgccag ccagctctca
     1021 tgctgcacct tcaggtcgcc gcgttgccgg gccacccgat cgcggccttg cgcgacatag
     1081 cgaatgcgct cctcgatccg gctgacctcg agcgccgcct cgccgagcgc tccctgcgcg
     1141 gcgtgcaact cgtcattggc cgcatagtgc gactggcgaa tctgttcgag ctcggcctcg
     1201 gcgtgacgca gctcggcaag cttcgactcg agcgccgcgc tcgccgcgac gagcccttga
     1261 cggacgcgct cttcctcgcg cgaagcatcg cggtgcttga ggaaccacag ctggtgctgg
     1321 cgtgtctgcg cgtcctgctg caaggcgcgg tagcggccag ccacctcggc ctggccctgg
     1381 agcttgtcga ggttttgccc gagctcgcgc aggatgtcct cgacgcgggt caggttctcg
     1441 cgcgtgtcct tcaggcggtt ttcggtctcg cgacgacgct ccttgtattt ggagacgccg
     1501 gcggcctctt cgaggaacag gcgcaactcc tcgggcttgc tctcgatgat gcgcgagatc
     1561 gtgccctggc cgatgatcgc gtacgcccgc ggcccgagac cggtgccgag gaagacgtcc
     1621 tggacatcgc ggcgacgcac cgcctggttg ttgatgtgat agctgctcgt tccgtctcgc
     1681 gtcaggacac gcttgacggc aatttcggag aaccggctcc agggtccgcc ggcgcgtccg
     1741 tcgacgttgt cgaagaccag ctcgacgctg gcgcgaccgg ccggctttct caggctcgag
     1801 ccgttgaaga tcacgtcctg catcgactcg ccgcgcagtt cgctcgcctt gctctcgccc
     1861 agaacccacc gaaccgcgtc gatgatgttc gacttgccgc agccgttcgg accgacgacg
     1921 ccgacgagct ggccgggaag ctggaactgg gtcggttcga cgaaggactt gaagcccgag
     1981 agcttgatcg agttgagacg catggacggc ggcgagcgga gagcctaagt tattgattta
     2041 tttaagaaaa acgccgccgt ttggcaagcc cgaatcgttc ggatgatagc atcgcggccc
     2101 acctcccacg ctcgtgtcgg caccgccccg gaccatggcc aagacctccc gcaacgccct
     2161 ttccgtctcg acgcggcggc cgcccgccac gccgccgagc gcgccgcaaa agcatctcga
     2221 cgtctttgcc aatccggcac ccgggcgcga ctacctgatc gagttcgaga tccccgagtt
     2281 cacgtgcctg tgcccgctga ccggccaacc cgatttcgcc cgcttcacga tcgaagtgat
     2341 cgccgatcgc ctgtgcatcg agttgaagag cctgaagctc tacctgtgga gctttcgcga
     2401 cgtcggcgcc ttccacgaga aggtcaccaa cgcgatcctc gacgacatcg tctcggcgac
     2461 gaagccgagg ttcgcccgca tccgcgcgca ctggaacgtg cgcggcggca tcgtcacgaa
     2521 cgtcgcggcc gagtaccgcg ccaagggctg gaaggcgaag tcgccggcat tgctggggta
     2581 gtcgcccgtt cgccgacacg tcggacgtgt gcgatggccg caagagtgcg gtggcacatc
     2641 gtgtggtccg aaaggcggcg cctcaggcca cagcgagact gcgccggtag tcgacgaaca
     2701 ggccgccgcc gacgtcttcc ctgagcgaag cccagtccgg cgccgacgac tcgatctcgg
     2761 cgatcgcggc gcgcggcagg ccgacgacgt cgagcaattc ctcggtcgcg gtccggatcc
     2821 agccgagcat cagcttgtcg gcttgcgcat cgacgagtcg aaactcgacg gtcgaagcga
     2881 ccggcgcccg gtcgagatgg cggatgacgc cgagcttggc gagctcgagc gtgtggtcgt
     2941 cgattcccgc ctgcgcggcg agcagcttct cgccgagccc ggcccagccg aacacggccc
     3001 gtgccgccat cttcgcaccc agcgcgcgcg cctccttcgg cgccgatcga ccgaacgacc
     3061 ggtcgaacgc ctcctggctc ttcttttcca gctcgggcca ctcggcgagc cgcgacgccg
     3121 gccagacggc g
//
LOCUS       DASRWS010000515         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_217992, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000515 DASRWS010000000
VERSION     DASRWS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217992"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFF72_09655"
     CDS             <1..560
                     /locus_tag="VFF72_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HZV93472.1"
                     /translation="SAINRVLVSRAVRLLALRGDDSVIDFFCGLGNFTLPLARGARSV
                     LGIEGSAALLERASDAAMLNGLEARVKFLARNLFEVGADDLVGFGPARKWLLDPPREG
                     AFAIVKSLVECIDERPPSVEWQPPERIVYVSCNPATLARDAGLLVHRGGYACTAAAVV
                     NMFPHTAHVESMAVFERSPEKEKGP"
     gene            complement(613..1311)
                     /locus_tag="VFF72_09660"
     CDS             complement(613..1311)
                     /locus_tag="VFF72_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZV93473.1"
                     /translation="MDPSQQAVQMGYAVPVTTVEERNRVLRNTYWLLALSMVPTVLGA
                     WIGVTTGLMTTMRPGMSMIVFLGGAFGFIFAIEKTKNSAAGVPVLLAFTFFMGLMLSR
                     LLSFVLGFSNGAGLVMTAFAGTGAIFLGMATLSTVIKRDLSAMGKFLFIGAVMVLVAM
                     IANLFLQSSALMVTLSVLVVGIFSAFIVYDLQRVKNGMETNYISATLSVYISLYNIFT
                     NLLALLGIFGGNRN"
     gene            1709..>2635
                     /gene="carA"
                     /locus_tag="VFF72_09665"
     CDS             1709..>2635
                     /gene="carA"
                     /locus_tag="VFF72_09665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZV93474.1"
                     /translation="MLPDHAPALLALADGTVFHGVSIGADGETTGEVVFNTSLTGYQE
                     ILTDPSYCRQIVTLTYPHIGNYGSNDEDRESRRVFASGLVVKDVPQLASNFRSTASLR
                     DYLRREGTVAIAGIDTRRLTRLLRTGGAQNGCIVALPPGAAIEAGRIDNAVDSARRAP
                     SMAGLDLAKVVSVGEPYEWTQTSWSLGSGYGEQRAPRLHVVAYDFGVKFNILRMLAAR
                     GCRITVLPARTSSAQALALSPDGVFLANGPGDPEPCDYAIEATRDFIARGVPTFGICL
                     GHQIMALACGAKTYKMKFGHHGANHPVKDLDDG"
BASE COUNT          464 a          883 c          857 g          431 t
ORIGIN      
        1 actcggcgat caaccgggtc ctggtctcgc gcgccgttcg cctgctcgcg ctgcgcggcg
       61 acgactcggt gatcgacttc ttctgcggcc tcggcaattt caccctgcca ctggcaaggg
      121 gcgcgcgctc cgtgctggga atcgagggga gtgcggcgtt gctggaacgc gcgagtgatg
      181 cggcgatgct caacggcctc gaagcgcggg tgaagttcct ggcacgcaac ctgttcgagg
      241 tcggcgccga cgatcttgtc gggttcgggc cggctcgcaa gtggcttctc gacccgccgc
      301 gcgaaggcgc cttcgcgatc gtcaagtcgc tggtcgagtg catcgacgaa cggccgcctt
      361 ctgtcgagtg gcagcccccc gagcggatcg tgtacgtgag ctgcaatccg gcgacgctcg
      421 cacgcgatgc cggactactg gtgcaccggg gcggctacgc ctgcacggcc gccgcggtcg
      481 tcaacatgtt cccccacacg gcccacgtcg agagcatggc ggtgttcgag cgttcgccgg
      541 aaaaggaaaa gggcccctaa ggaccctttc ctggaaggag gccgcgaacg gcctccgaat
      601 gcgcgagcaa gctcagttgc ggttgccgcc gaagatgccg agcagcgcga gcaggttggt
      661 gaagatgttg tagaggctga tgtagacgct cagcgttgca ctgatgtagt tcgtctccat
      721 gccgttcttg acccgctgca ggtcgtacac gatgaacgcc gagaagattc cgacgacgag
      781 aaccgaaagc gtcaccatca acgcgctcga ctgcaggaac aggttggcga tcatcgccac
      841 cagcaccatg acggcgccga tgaacaggaa cttgcccatg gccgacaggt cgcgcttgat
      901 gacggtggac agcgttgcca tcccgaggaa gatcgcgccg gtgcctgcaa aggccgtcat
      961 cacgaggccg gcgccgttcg agaagccgag cacgaagctc agcagccgcg acagcatcag
     1021 gcccatgaag aacgtgaacg ccagcagcac gggaacgcct gctgcggagt tcttcgtctt
     1081 ctcgatcgcg aagatgaagc cgaatgcgcc gccgaggaag acgatcatgc tcatgcccgg
     1141 ccgcatcgtc gtcatgaggc ccgtcgtcac gccgatccag gcgccgagca ccgtcggcac
     1201 catcgacagg gcgagcaacc agtaggtgtt gcgaaggacg cggttgcgct cctcgacggt
     1261 ggtgaccggg acggcgtagc ccatctgaac ggcttgctgg ctgggatcca tgggacctcc
     1321 tgatgtgacg aacagtctga cgggaatctg acccgcttgc agggattcta gttcccgcat
     1381 ggtgacacat tgcgaaggga acggacattt cagccccccc ccgaacgcca tccacatgcg
     1441 ggcggtcgag ccgattccaa gagcgacgcc cggatcggtt cgctcgacgg gacgacgcgc
     1501 acgccctgga gcttgccgag ccgaagcccg gcgagaagtc gggtcgtcgt cggcgcggct
     1561 acaatttgtc gcctcagccc gctttgcgat gcaggttgaa gcggctgcag caaggcaccg
     1621 ttcggcgcca tgctcgcgcc ctcagggacg gcgcctgccg tccaagccgc ctcgcatcga
     1681 cccggagctc ttttccttgt tcccgcaact gctgcccgac cacgccccgg ccctgctggc
     1741 cctcgcagac ggcacggtgt tccatggtgt ctcgatcggc gcggacggcg agacgacggg
     1801 cgaggtcgtt ttcaacacct cgctcaccgg ctaccaggaa atcctgacgg atccgagcta
     1861 ttgccggcag atcgtcacgc tcacgtatcc gcacatcggc aactacggct cgaacgacga
     1921 ggaccgggaa tcgcggcgcg tcttcgcctc cggcctcgtc gtcaaggacg tgccgcagct
     1981 cgcttcgaac tttcgcagca cggcatcgct gcgcgactac ctgcgccgcg aaggcacggt
     2041 tgccatcgcc gggatcgaca cccggcgtct cacccgcctg ctgcgcacgg gtggcgcgca
     2101 gaacggctgc atcgtcgcgc tgccccccgg tgcagcgatc gaagcaggtc gcatcgacaa
     2161 cgcggtcgac agcgcgcgtc gcgcgccgtc gatggccgga ctcgatctcg ccaaagtggt
     2221 cagcgtcggc gagccctacg aatggacgca gacgtcgtgg tcgctcggca gcgggtacgg
     2281 ggagcagcgc gcgcctcggc tgcatgtcgt cgcctacgac ttcggcgtca agttcaacat
     2341 cctcaggatg ctcgccgcgc gaggttgccg gatcaccgtg ctgccggctc gcacgtcgag
     2401 cgcgcaggcg ctcgccctca gccccgacgg cgtctttctc gccaacgggc ccggcgaccc
     2461 cgagccctgc gactacgcga tcgaggcgac gcgtgatttc atcgcccgcg gcgtgccgac
     2521 cttcggcatc tgcctgggtc accagatcat ggcgctggcc tgcggcgcaa agacgtacaa
     2581 gatgaagttc ggccaccacg gcgccaatca cccggtcaag gatctcgacg acggt
//
LOCUS       DASRWS010000516         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2794654, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000516 DASRWS010000000
VERSION     DASRWS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2794654"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFF72_09670"
     CDS             <1..207
                     /locus_tag="VFF72_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93475.1"
                     /translation="WHAERRQGSLAFYNWFAYEFWRFVLVQEQVGKLAQTAIDFPPMQ
                     KGASFNLEAVKAQIEAAMKSRTGD"
     gene            complement(490..>2129)
                     /locus_tag="VFF72_09675"
     CDS             complement(490..>2129)
                     /locus_tag="VFF72_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HZV93476.1"
                     /translation="SRRAFESGIPLRSAVSVRHARRVLCVFPAYTPSFGTFQHAFALM
                     GRVRAFMPPQGLLLIAAYMPQAWPVRFIDENITPATDADFAWADVVFVSGMHIQAPQI
                     RDICARAHAAGRVVALGGPSVSSAPEMYPDFDYLHVGEIGDATDDLIARLDASVARPA
                     AQVTLKTQERLGLDAFPLPAYDMIPLARYLIGSLQFSSGCPYRCEFCDIPGLYGRQPR
                     LKSPEQLTGELEAMMRQPTRPMVIYFVDDNFIGNKKAARDMLHHLIAWQKQHYYPFAF
                     ACEATLNLAKQTEILALMREAGFCEVFVGIETPDADALKGMKKDQNLMVPLLESVATL
                     NSYGLEVTAGMIIGLDTDKPDTDQHIIDLIDQSNIPILTLNLLQALPKTPLWDRLAKA
                     GRLVNDPMLESNVRFLRPHDDVVASWRRCVGHAYDPKRLFARYMHQVEATYPKRLRPP
                     ARGRLNWPSVKLGATLAWNVATRVGWRSDYRREFWGTAWPALKRGQIDAVLGMGFTSH
                     HLIQFSREALRGEQNASFYSTKAREQGEAQEAAEAAA"
BASE COUNT          353 a          668 c          760 g          348 t
ORIGIN      
        1 tggcatgccg agcggagaca aggctcgctg gccttttaca actggtttgc ctacgagttc
       61 tggcgcttcg tgctcgtgca ggagcaggtc gggaagctcg cgcagaccgc gatcgacttc
      121 ccgcccatgc agaagggggc gagcttcaat ctggaggccg tcaaggcgca gatcgaggcg
      181 gccatgaagt cgcgcacagg cgactgacga tgcggggcga gtgggcccgg cgcattttcc
      241 gctcgtcgtc ttgaagcgcc ggccggagag gtccggcggc ggcggcagac ccgtcgccgg
      301 gagcgatcag gacatcagga gaatgacgtg aacattcgct gacgcgaagg atcgtcgcga
      361 gcgcgatcgc cgtgctgctc gtcgctgccg gcgccgtcac gccggagatt gcggtcgggc
      421 gcagcccatt ggcgtcgccg cttcatggcg cgttcatgac gagcaagcga gtgcggcatg
      481 cgccgcgcct cacgccgccg cttcggccgc ctcttgcgcc tcgccctgct cgcgcgcctt
      541 ggtcgaatag aacgacgcgt tctgctcgcc gcgcagggcc tcgcgcgaga actggatcag
      601 gtggtgcgac gtaaatccca tgccgagcac ggcgtcgatc tggccgcgct tgagcgccgg
      661 ccaggcggtg ccccagaatt cgcggcgata atcggagcgc cagccgacgc gcgtcgcgac
      721 gttccaggcg agcgtggcgc cgagcttgac gctcggccag ttgaggcggc cgcgcgccgg
      781 cggccgcagg cgcttcgggt aagtcgcctc gacctggtgc atatagcgcg cgaacaggcg
      841 cttgggatcg taggcgtgcc cgacgcagcg ccgccacgac gcgacgacgt cgtcgtgcgg
      901 gcgtaggaag cgcacgttcg actcgagcat cggatcgttg acgagccgcc ctgctttggc
      961 gaggcggtcc cacaacggcg tcttgggcag cgcctgcagc agattaagcg taaggatcgg
     1021 aatgttggac tgatcgatca aatcgatgat gtgctggtcg gtgtcgggct tgtcggtgtc
     1081 gaggccgatg atcatgccgg ccgtcacctc gaggccgtag ctgttgagcg tcgcgaccga
     1141 ctcgagcagc gggaccatca ggttctggtc tttcttcatg cccttgagcg cgtcggcgtc
     1201 cggcgtctcg atgccgacga acacctcgca gaagccggcc tcgcgcatca gcgcgaggat
     1261 ctcggtctgc ttggcgagat tgagcgtcgc ttcgcacgcg aaggcgaacg gataatagtg
     1321 ctgcttctgc cacgcgatca ggtggtgcag catatcccgc gcggcttttt tgttgccaat
     1381 gaagttgtcg tcgacgaaat agatcaccat cggccgcgtc ggctggcgca tcatcgcctc
     1441 gagttcgccg gtgagctgct ccggcgactt gagccgcggc tggcggccgt agaggcccgg
     1501 gatatcgcag aactcgcagc gataggggca gcccgaggag aactgcaggc tgccgatgag
     1561 atagcgcgcg agcgggatca tgtcgtaggc gggcagcggg aaggcgtcga ggccgaggcg
     1621 ctcctgcgtc ttgagcgtca cctgcgcggc cgggcgcgcg acgctcgcat cgaggcgcgc
     1681 gatcaggtcg tcggtcgcgt cgccgatctc gccgacatgc aggtagtcga agtccggata
     1741 catctcgggc gcgctcgaga ccgaggggcc gccgagcgcc acgaccctgc cggccgcatg
     1801 ggcgcgcgcg cagatgtcgc ggatctgcgg tgcctggatg tgcatgccgc tgacgaacac
     1861 gacgtcggcc caggcgaaat cggcgtcggt cgccggcgtg atgttctcgt cgatgaagcg
     1921 gaccggccac gcctgcggca tataggcggc gatcagcagc aggccctgcg gcggcatgaa
     1981 ggcgcgcaca cgccccatca gcgcgaacgc gtgctggaac gttccgaacg acggcgtgta
     2041 ggccgggaag acgcacagca cgcgacgggc gtgccgaaca gataccgcag accgcaatgg
     2101 aatccccgat tcgaatgccc ttcgggact
//
LOCUS       DASRWS010000517         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2482397, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000517 DASRWS010000000
VERSION     DASRWS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482397"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..772)
                     /locus_tag="VFF72_09680"
     CDS             complement(2..772)
                     /locus_tag="VFF72_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93477.1"
                     /translation="MPSPLFDLTGKVAVVTGSSKGIGKAIAMHLALAGARVVVSSRKA
                     PVCEQAADEIRKAGGDATVIPCNISDKAQVEKLIAETKKQLGPVDVLVCNAASNPYYG
                     PNEKLPDDVFMKVMQNNILSNMWLINQCLPDMRAKKDGSIMIVSSIGGVRASTVIGAY
                     CISKAADMQLARNLAAEYGPDNIRVNTIAPGLIVTDFARALYEDPTKRAAREQATPLR
                     RLGQPDDIAGLAVMLAGKAGAFITGQTLIVDAGVTIGS"
     gene            complement(829..1893)
                     /locus_tag="VFF72_09685"
     CDS             complement(829..1893)
                     /locus_tag="VFF72_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZV93478.1"
                     /translation="MIIDRIDPIALRIPAAGGNTLCLTLSRVVTRDGVEGYGECLSLR
                     PPMQRALFATIRDAIAPHYLGKSVDDREALNLAARKRFASFGRAGTVLNALASVDIAL
                     WDIAGKVANKSVSELLGSARRSQVPVMASLDKYDDAKRTRARVEEALATGVAAVKVHE
                     HRLEVAEESRRVIPAAMPYVVDCNNAHTLADIKRDEKRWAAMNLLFFEDPLWPPEELL
                     SMSALPGITIGMGADFGSAEQMAIYSKTPAIGVLQPDVCMLGGLSEAKRALAMLAAAK
                     RTAAPHTPFVGPAALASLHMLAVAEGEGYFATVEADDSMDPYGFGHTRWKASLDMPTA
                     PGLGFDPDPGFLNRYDYAKE"
     gene            1886..2794
                     /locus_tag="VFF72_09690"
     CDS             1886..2794
                     /locus_tag="VFF72_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV93479.1"
                     /translation="MIMARSLTVPGAAASVFVDIGFDDLGGEYRMAADTFTTSDGIRL
                     AYYVDDFTDPWRQADTLLLLHAAVGHARRFYAWVPPLCRHYRVVRLDLRGHGASQVPS
                     ESSTLDMARLVADVEELLAHLGVKACHIVGNSAGGYIAQNLAMKSPDKVKSLILFGST
                     AGLRNSQAASWLPRIAKDGLRGFFRETIAERLPLDQVPKGLVDWFVDETGNNDPAWIA
                     RFVGYMSQQEWSDRLPAIKCPTLLVLPGGETVGSIRNYDVMIERIPDVQAITYEGLPH
                     NICDAVPDRCAADVLAFLRWRFGLPA"
BASE COUNT          458 a          947 c          943 g          489 t
ORIGIN      
        1 gtcagctccc gatggtcacg ccagcgtcga caatgagggt ctggccggtg atgaaggcgc
       61 cagccttgcc ggccagcatc acggcgagtc cggcgatgtc gtcgggctgg ccgagacggc
      121 gcaacggcgt cgcctgctcg cgcgcagcgc gcttcgtcgg atcctcgtac agcgcgcggg
      181 caaagtcggt tacgatcagg ccgggcgcga tcgtgttcac gcggatgttg tcgggcccgt
      241 actcggcggc gaggttgcgc gcgagctgca tgtcggccgc tttggagatg cagtaggcgc
      301 cgatcaccgt gctggcccgc acgccgccga tcgacgacac gatcatgatc gagccgtcct
      361 tcttggcgcg catgtcgggc aggcactggt tgattagcca catgttcgac aggatgttgt
      421 tctgcatcac cttcatgaac acgtcatcgg gcagcttctc gttcggcccg taatacgggt
      481 tcgacgcggc gttgcagacc agcacgtcga ccggcccgag ctgcttcttc gtctcggcga
      541 tcagcttctc gacctgcgcc ttgtcggaga tgttgcaggg aatgacggtc gcatcgccgc
      601 cggccttgcg gatctcatcg gccgcctgct cgcagaccgg tgccttgcgc gacgacacca
      661 cgacccgggc gccggcgagc gcgagatgca tggcgatcgc cttgccgatg cccttgctcg
      721 agccggtgac gacggcgacc ttgccagtga ggtcgaaaag cggcgatggc atcgtgtttc
      781 ctccgattca gctttcctct ccattcagat ggggaggaac aagacctttc attccttcgc
      841 atagtcgtac cggttcagga agcccggatc aggatcgaag ccgaggcccg gggcggtcgg
      901 catgtcgagc gaggccttcc agcgcgtgtg accgaagccg tagggatcca tcgaatcatc
      961 ggcctcgacg gtggcgaagt agccctcgcc ctccgcgacc gccagcatgt gcagcgaggc
     1021 gagcgccgcc gggccgacga acggcgtgtg aggcgcggcg gtcctcttcg ccgcggccag
     1081 catcgccagc gcgcgtttgg cctcgctgag gccgcccagc atgcagacgt cgggctgcag
     1141 cacgccgatc gccggcgtct tgctgtagat cgccatctgc tcggccgagc cgaaatcagc
     1201 gcccatgccg atggtgatgc cgggcagcgc cgacatcgac agcagctctt ccggcggcca
     1261 cagcggatcc tcgaagaaca gaaggttcat cgccgcccag cgcttctcgt cgcgtttgat
     1321 gtcggccagc gtgtgggcgt tgttgcagtc gacgacgtag ggcatcgccg ccggaatgac
     1381 gcggcgcgat tcctcggcaa cctcgagccg gtgctcgtgc accttcaccg ccgccacgcc
     1441 ggtcgccagc gcctcctcga cccgcgcccg cgtgcgcttc gcgtcgtcgt acttgtcgag
     1501 gctcgccatc accggaacct ggctgcgcct ggcacttccg agcaattcgg acaccgactt
     1561 gttcgccacc ttgccggcga tatcccacag cgcgatgtcg accgaagcca acgcgttcag
     1621 caccgtgccg gcccggccga agctcgcgaa gcgcttgcgc gcagcgaggt tcagcgcctc
     1681 gcgatcgtcg accgacttgc cgagatagtg cggcgcgatc gcgtcgcgga tcgtggcgaa
     1741 cagcgcccgc tgcatcggcg gtcgcagcga caggcattcg ccgtagccct cgacgccgtc
     1801 gcgggtcacc acgcggctca gggtgagaca gagcgtgttg ccgccggcag cgggaatgcg
     1861 aagcgcaatg gggtcgatgc gatcgatgat catggcacgc agtttgacag tgcccggagc
     1921 cgctgccagc gttttcgtcg atattgggtt cgatgatctt gggggagagt accgcatggc
     1981 ggccgacacg ttcacgacca gcgacggcat caggctcgcc tactacgtcg atgactttac
     2041 cgacccgtgg cggcaggccg acacactgct tctgctccat gccgccgtcg gccacgccag
     2101 gcgcttctac gcctgggtgc caccgctctg ccggcactat cgcgtggtgc ggctcgacct
     2161 gcgcggccat ggcgcctcgc aggtgccgtc ggagtcctcg acgctcgaca tggcgcgact
     2221 ggtggccgac gtcgaggagc tgctggcgca tctcggcgtc aaggcgtgcc acatcgtcgg
     2281 caactcggcc ggcggctaca tcgcccagaa cctggcgatg aagtcgccgg acaaggtgaa
     2341 gagcctgata ctgttcggct cgaccgccgg cctgcgcaac agccaggccg cgagctggct
     2401 gccgcgcatc gccaaggacg ggctgcgcgg cttcttccgg gagaccatcg ccgagcgcct
     2461 gccgctcgac caggtgccga aggggctggt cgactggttc gtcgacgaga ccggcaacaa
     2521 cgatccggcc tggatcgccc gtttcgtcgg ctacatgagc cagcaggaat ggagcgacag
     2581 gctgccggcg atcaagtgcc cgaccctgct cgtcctgccg ggcggggaga ccgtcggctc
     2641 gatcaggaac tacgacgtca tgatcgaacg cataccggac gtgcaggcga ttacctacga
     2701 aggcctgccg cacaatattt gcgatgcggt gccggaccgt tgcgcggccg acgttctggc
     2761 attcctgcgt tggcggttcg gcctaccggc gtaacaagcc ggcgtgataa aagagggcat
     2821 ggaccagacc atcgccc
//
LOCUS       DASRWS010000518         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2482601, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000518 DASRWS010000000
VERSION     DASRWS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482601"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFF72_09695"
     CDS             <1..339
                     /locus_tag="VFF72_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93480.1"
                     /translation="TGSWDAGRLHQVLANLVVNALKYGSGQAPIVIRLDGSENDVVFS
                     VHNQGDVIAQATLDRMFAPLTRGSDAGQREGSMGLGLYICREIVVAHKGEISSASDAS
                     GTTFTVRLPR"
     gene            complement(383..1264)
                     /locus_tag="VFF72_09700"
     CDS             complement(383..1264)
                     /locus_tag="VFF72_09700"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZV93481.1"
                     /translation="MHIETALDAKAVIGESPTWVAKENALYWIDVKAPALHRFRPADG
                     ATRTWPLGSDVGAFAFLPDGAALVALRHGIHRLDLASGSSTLLAPPPFDPALFRFNEG
                     ACDAAGRFWVGVMFDPVSGKPEKQPGRLHSFTIAEGLRAEPDAAELHNGIAWSDDGRT
                     FFLSHSDSGEIFAFDYEPAHGVVSNRRVFARIPGELGIPDGAAIDTRGGYWCALHGGG
                     KIRRFDPDGSVAEDIELPVSQPTMCAFAGDGLSTLYITSASDKMSAEQKRQEPRAGSL
                     LKAVTQARGIVRPFVVR"
     gene            complement(1300..>2174)
                     /locus_tag="VFF72_09705"
     CDS             complement(1300..>2174)
                     /locus_tag="VFF72_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZV93482.1"
                     /translation="AMVGRIEMGAAGRATGVRYHRQGAWHFQRARHVVVAGYAVESPR
                     LLLNSATAQFPDGLANRSGLVGRNLMTQSNQAVWGRMDEEVRWYKGPPSLAITEHWNY
                     DDRKDFHGGYCWMGQGPLPNEWAAVQTGARGLWGRPLRDAMRDYIHMIGVKMVGEMLP
                     DEANRVTLADDTDQYGLRVARITYRWGDNDKALIAHALEQMQRSIEAIGARDIFRQDD
                     DTCHLGGTVRMGSDRDTSVVDADCRSWDIPNLWVCDGSVFPTVGGVNPALTIQTVAMR
                     TADRIGQLAAAGDL"
BASE COUNT          328 a          749 c          748 g          349 t
ORIGIN      
        1 accggctcgt gggacgccgg ccgccttcac caggtgctcg cgaacctcgt cgtcaacgcc
       61 ttgaaatacg gaagcgggca ggctcccatc gtgatccgcc tggacggaag cgagaacgac
      121 gtggtgttct cggtgcacaa ccagggcgac gtcatcgcgc aggccacgct cgaccggatg
      181 ttcgctccgc tgacccgggg cagcgacgcc ggccagcgag aaggaagcat gggcctgggc
      241 ctgtacatct gccgcgagat cgtcgtcgcc cacaagggcg agatttcgtc ggcctccgac
      301 gcgtcgggca cgaccttcac cgtccgcctg ccacgatagg ccgggcggcg cgacccggcg
      361 tcacgccgtc ggctcgtcct gcctagcgaa cgacgaatgg cctgacgatg ccgcgcgcct
      421 gcgtcaccgc tttcagcagg ctgccggctc gcggttcctg gcgcttctgc tcggcgctca
      481 tcttgtcgga agcgctcgtg atgtagagcg tcgacaagcc gtcaccggcg aatgcgcaca
      541 tcgtcggctg gctcacgggc agctcgatgt cttcggcgac cgagccgtcg gggtcgaatc
      601 gcctgatctt tccgccgccg tgcagtgcgc accaatagcc gccgcgggta tcgatcgcgg
      661 caccgtccgg aattccgagc tcgcccggaa ttcgcgcgaa caccctgcgg ttcgacacca
      721 cgccgtgagc cggctcgtaa tcgaacgcga agatctcgcc ggagtcgctg tgcgacaaaa
      781 agaaggtgcg gccgtcgtcg ctccaggcga tgccgttgtg cagctcggcg gcgtcgggct
      841 cggcgcgcag cccttcggcg atggtgaagc tgtgcagccg gccgggctgc ttctccggct
      901 tgcccgagac cggatcgaac atcacgccga cccagaatcg gccggcggcg tcgcacgcac
      961 cttcgttgaa gcgaaagagc gccgggtcga acggcggcgg cgccagcagc gtcgagctgc
     1021 cgctggcgag atcgagccgg tggatgccgt ggcgcaaggc gacgagtgcc gcgccgtcgg
     1081 gcaggaaggc gaaggcgccg acgtcgctgc cgagcggcca ggtccgcgtg gcgccgtcgg
     1141 ccggccggaa gcgatgcagc gccggcgctt tcacgtcgat ccagtagagc gcgttctcct
     1201 tggcgaccca ggtcggcgac tcgccgatca cggccttggc gtcgagagcc gtttcgatgt
     1261 gcatgacgct tctccgtggg acggccgaac cttcgtgcgc tacagatcgc cagccgcggc
     1321 gagctggccg atgcgatcgg ccgttcgcat cgccaccgtc tggatcgtca gtgccgggtt
     1381 gacgccgccc accgtgggaa acaccgagcc gtcgcagacc cagagattgg gaatgtccca
     1441 gctgcggcag tcggcatcga cgacgctggt gtcgcgatcg ctacccatgc gaaccgtgcc
     1501 gccgagatgg caggtgtcat cgtcctgccg gaagatgtcg cgcgcgccga tcgcctcgat
     1561 cgagcgctgc atctgctcga gcgcgtgcgc gatcaaggcc ttgtcattgt cgccccagcg
     1621 ataggtgatc ctggccacgc gcaggccgta ctggtcggtg tcgtcggcga gggtgacgcg
     1681 gttggcttcg tcgggcagca tctcgccgac catcttgacg ccgatcatgt ggatgtagtc
     1741 gcgcatcgcg tcgcgaagcg gccggcccca gaggccgcgg gcgcccgtct gcaccgccgc
     1801 ccactcgttc ggcagcggcc cctgtcccat ccagcaatag ccgccgtgga agtccttgcg
     1861 gtcgtcgtag ttccagtgct cggtgatggc caggctcggc ggtcccttgt accagcggac
     1921 ctcctcgtcc atgcgccccc agaccgcctg gttcgactgc gtcatcaggt tcctgccgac
     1981 cagcccggaa cggttggcca ggccatcggg aaattgcgcg gtggccgagt tgaggagcag
     2041 ccgcggcgac tcgacggcgt agccggcgac gacgacgtgc ctcgcgcgct ggaagtgcca
     2101 ggcgccctgg cggtgatagc ggacgccggt cgcgcggccg gcggcgccca tctcgatccg
     2161 gccgaccatc gcca
//
LOCUS       DASRWS010000519         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2483024, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000519 DASRWS010000000
VERSION     DASRWS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483024"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1265)
                     /locus_tag="VFF72_09710"
     CDS             complement(264..1265)
                     /locus_tag="VFF72_09710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZV93483.1"
                     /translation="MKAFVVDRYQKQTALRLADLPTAEPREDEVLVEVHAAGVNLLDA
                     KIRSGEFKLILPYRLPLVLGHDVAGVVAKVGTRVRQFKVGDEVYARADDFRIGTFAEF
                     VAVKEASLALKPRGLTMEEAASIPLVGLTAWQALVEKAGLKKGQKVFIQAGSGGVGTF
                     AIQLAKHLGATVATTTSTTNVALVKSLGADVVIDYKTQDFEDVLRDYDVVLNSQDGKT
                     LEKSLRILQRGGQLISISGPPDPQFGRQIAAPGFVRLVMRLLSSGVRRKARQLGVGYS
                     FLFMRANGSQLREITRLIDGGAIRPVMDRVFPFSSTNEALTYVEAGRAKGKVVLRLK"
     gene            complement(1262..2101)
                     /locus_tag="VFF72_09715"
     CDS             complement(1262..2101)
                     /locus_tag="VFF72_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZV93484.1"
                     /translation="MKSRSNKIALVTGASSGIGQATAQLLAEAGYTVYGTSRRGGAAG
                     GRSFQMLSLDVTSDASVAAAVAQVMQREGCIDLLVNNAGFGVAPAGAEESSIAQAQSI
                     FDTNFFGIVRMTRAVLPHMREQGAGRIINIGSVLGLLPMPYGALYAATKHAIEGYSES
                     LDHELRTRGIRVSVIEPAYTNTPFDANFLQPDAMLAAYGEARAAVGQRVKEVMATADQ
                     PGIVAEVVLKAASAVRPQLRYTAGGLAGRLTWLRRFAPAGLMDAGIRRDLRLDATLAG
                     RSS"
     gene            complement(2120..2638)
                     /locus_tag="VFF72_09720"
     CDS             complement(2120..2638)
                     /locus_tag="VFF72_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93485.1"
                     /translation="MDATLSGAAAASDPTLRPGSPWPMFWIASVAIFLVSLDGTMLYS
                     VFGALRAGFPGASAADMSWVLQRGDAASLTFGIAFAMMFFAFFFYMGNVWHYSPARAE
                     LAIAPGPLTVIPAAMLTGRFAARFGHRHEGGHCPIARSTTSARIAKGAAGLRRRSDRL
                     DEQTRFAELPTT"
     gene            complement(2674..3393)
                     /locus_tag="VFF72_09725"
     CDS             complement(2674..3393)
                     /locus_tag="VFF72_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93486.1"
                     /translation="MKIANAVALVTGANRGIGLAFTRALLARGARKRGARKVYAGARD
                     PSTITEPDVEPIRLDVTKPDEVAAAAARARDVTLVINNAGIGQFGGFLAPDSEESARR
                     HLETNFFGMLRMSHAFAPLLAANGGGALLNVLSIVSWINGGQLAAYAASKSAAWSLTN
                     ALRHELAAQRTQVLALHMAFVDTDLVRALDGPKASPDEIVARALDGLEDGLDEVLADE
                     RTRLVKQGLTATRPSYLPQLA"
     gene            complement(3489..4085)
                     /locus_tag="VFF72_09730"
     CDS             complement(3489..4085)
                     /locus_tag="VFF72_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV93487.1"
                     /translation="MSRTPNSRKQETHERIVNVAARAIRRHGYAGVGVADVMKEAGLT
                     HGGFYAHFESRDALLVEALERAGRESGEAVTRALERRAARGVSAFRALVEAYLGDEHL
                     ASLEIGCPVAALACDMPRQSDAVREASAVRVRRLVAGVQSALPHASRATASVVAGTLV
                     GTLQLARALGANADGRALLSAARKALVQQYDTPSTAAG"
BASE COUNT          711 a         1391 c         1397 g          727 t
ORIGIN      
        1 atcttgcgcg aagtggtcag cccgctgggg agactgcagc ccgcgtaggt cctcctcatg
       61 gcgcactgcg ctctggaggt tgccgacatc ttcgatgacg cggtcgtcgc taccgcgctc
      121 ctgcgaccgg cgcgcgccag ccagttgccg agcgctgccc ccatgtctgg gaaaacaaat
      181 cgtgcaccgc ttcccggggg agtagacgtt cgtagtcgcc gatcgcctgc aactccgagc
      241 cccgcgcgcc agaaagagga gcctcatttc agcctgagga cgacctttcc cttcgcgcgt
      301 ccggcctcga cgtaagtcag cgcctcattg gtcgacgaga acggaaacac ccgatccatc
      361 acggggcgga tggccccgcc gtcgatgagt cgggtgatct cgcgcaattg gctcccattc
      421 gccctcatga agaggaatga gtagcccacg cccaattgcc gcgccttgcg cctcacgccc
      481 gagctcagca gccgcatcac cagtctcacg aatccgggtg ccgcgatctg gcgtccgaat
      541 tggggatcgg ggggcccgga gatggagatg agctggccgc cacgctggag gatgcgaaga
      601 gacttctcga gtgtcttgcc atcctggctg ttcagcacca catcgtagtc acgaagcaca
      661 tcctcgaaat cttgcgtctt gtagtcgatg acgacgtctg cccccaggct cttcacgagc
      721 gcgacgttgg ttgtactcgt ggtcgtagcg acggtggcgc ccagatgttt ggccagctgg
      781 atggcgaagg tgcccacgcc gcccgagccg gcctggatga agaccttctg gcccttcttc
      841 aggccggcct tctcgacaag cgcctgccat gccgtcaacc ccaccagcgg aatggaagcg
      901 gcttcctcca tcgtcaggcc cctgggcttg agcgcaagcg acgcctcctt gaccgcgacg
      961 aattctgcga acgtgccgat ccggaagtca tccgcccgcg catagacctc atcacccact
     1021 ttgaactgcc gcacccgcgt gcccaccttg gccacgacac ctgccacgtc gtgtccgagg
     1081 acgagaggca ggcgatacgg caggatgagc ttgaactcgc cgctccggat cttggcgtcc
     1141 agcaggttca cgccggcggc atggacctcg accaggacct cgtcttcacg cggctcagcg
     1201 gtcggcaagt ccgccaggcg cagagcggtc tgcttttgat agcgatcgac gacgaatgcc
     1261 ttcacgatga tctccccgcc agtgttgcat cgagtcgcag atcccttcga atcccggcat
     1321 ccatcagtcc ggcaggcgcg aacctgcgca gccaggtcag ccggccagcg agcccgccgg
     1381 ctgtgtagcg aagttgcgga cgaaccgcac tggcagcctt cagcacgacc tcggccacga
     1441 tacccggctg gtctgcagtg gccatcactt ccttcacccg ctggccgacg gcggcgcggg
     1501 cctcgccgta ggcggcgagc atggcgtcgg gctgcaggaa gttcgcgtcg aaaggcgtgt
     1561 tcgtgtacgc cggctcgatg accgagaccc ggatccccct ggtgcgcaac tcatggtcga
     1621 gcgactcgga atagccttcg atcgcatgct tcgtcgcggc atagagcgcg ccgtagggca
     1681 tgggcagcag acccagaacc gaaccgatgt tgatgatgcg cccggcgccc tgttcgcgca
     1741 tgtgcggcag gacggcacgc gtcattcgga cgattccgaa gaagttggtg tcgaagatcg
     1801 actgggcctg ggcgatcgag ctttcttcgg ccccggcagg agcgacccca aagccggcgt
     1861 tgttgacgag gaggtcgatg caaccttccc gctgcatcac ttgcgcgaca gcggcggcga
     1921 cagacgcatc gctggttaca tccagggaca gcatctggaa cgatcgcccg cccgcagcgc
     1981 caccacgccg actcgtcccg tagaccgtgt agccagcctc tgcgaggagt tgcgcagtgg
     2041 cctgtccgat gcccgaggag gcacccgtca cgagcgcgat cttgttgctc cggcttttca
     2101 tgttcacgac gcttctccgt caggttgtgg gtagttcggc aaatcgagtc tgttcgtcga
     2161 ggcggtctga acggcgtcgt agacctgcag cgcctttcgc aatcctcgcg cttgtagtcg
     2221 acctagcgat cggacaatga ccgccttcat gtcggtggcc gaagcgagca gcgaagcgac
     2281 cggtgagcat ggcggccgga atcaccgtca gcgggcctgg cgcgatcgcc agctccgcgc
     2341 gcgccgggct gtagtgccag acgttgccca tgtagaagaa gaaagcgaag aacatcatcg
     2401 caaaggcgat gccgaaggtc agcgaggctg catcaccgcg ctgtaggacc cacgacatat
     2461 cggccgcgct cgcgccggga aaacccgcgc gcaacgcacc gaagaccgag tacagcatcg
     2521 tgccgtcgag cgagaccagg aagatcgcga cgctcgcgat ccagaacatc ggccacggcg
     2581 atccggggcg cagcgttggg tcggaggcgg cggcagcgcc ggacagggtg gcatccatcg
     2641 tgcgtgactt cggtgttgct gcgcgagcgc ggatcaggcg agctgcggca ggtaactcgg
     2701 ccgcgtcgcc gtcaggccct gcttgacgag tcgagtccgt tcgtcggcga gcacctcgtc
     2761 gagcccgtcc tcgagtccgt cgagcgcgcg ggcgacgatc tcgtcggggc tggccttcgg
     2821 gccgtcgagg gcgcgcacga ggtcggtatc gacgaaggcc atgtgcagcg ccagcacctg
     2881 cgtccgttgc gcggcgagct cgtgacgcag cgcgttcgtg agcgaccagg cggccgattt
     2941 gctggcagcg tacgcggcga gctgcccgcc gttgatccac gacacgatgg acagcacgtt
     3001 gagcagcgcg ccgccgccgt tagcggcgag cagcggtgcg aaggcgtggc tcatgcgcag
     3061 catgccgaag aagttggttt ccaggtgccg gcgtgcgctt tcctcgctgt ccggcgcgag
     3121 gaagccgccg aactggccga tgccagcgtt gttgatgacg agcgtcacgt cgcgggcgcg
     3181 cgcggcggcc gctgccacct cgtcgggctt cgtcacgtcc agccggatcg gctcgacatc
     3241 cggctcggtg atggtcgagg gatcacgcgc accggcgtag accttgcgcg cgccgcgctt
     3301 gcgcgcgccg cgggcaagca gcgcgcgtgt gaaggcgagg ccgatgccgc ggttggcgcc
     3361 agtgaccaag gcaacggcgt tggcgatctt catgaaaatc tcctgtgaga ggttgaggcg
     3421 gcgtccagta aatatgacag acatcatatt tgggggcgag tgagaggagc tggttttcgc
     3481 cgtgcggctc agccggccgc cgtgctgggt gtgtcgtact gctggacgag cgctttgcgt
     3541 gccgccgaca gcagcgcgcg cccgtcggcg ttggcgccca gggcacgcgc gagttgaagc
     3601 gttccgacga gcgtgccggc caccacgctc gccgttgcgc gcgatgcgtg aggcaaagct
     3661 gattgcactc cggcgacgag gcgccgcacg cgcaccgccg acgcctcgcg caccgcatct
     3721 gactggcgcg gcatgtcgca cgcgagcgcg gcgactggac agcctatctc gagagacgcg
     3781 aggtgctcgt cgccgagata cgcttcgacg agcgcccgga aggcgctcac gccccttgcc
     3841 gcccgtcgct cgagggcgcg cgtgaccgct tcaccgcttt cgcggccggc ccgctccagc
     3901 gcctcgacga gcagcgcgtc tcgagactcg aaatgagcat agaagccgcc gtgggtgagg
     3961 ccggcctcct tcatgacgtc agcgacgccg acaccggcgt agccgtggcg cctgatcgct
     4021 cgtgcggcga cgttaacgat ccgctcatgg gtctcctgct tgcggctgtt gggcgtgcgg
     4081 ctcatcaggg tcatccttat atgacgtcaa tcatattgct attgggatcg ctcgtcaacc
     4141 ccgtgccgca gggccagcct ttttgaaccc gtggcggatc gaatgccggc ttttagccca
     4201 gactgtcgcg aggtagttcc gcttcg
//
LOCUS       DASRWS010000520         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1667506, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000520 DASRWS010000000
VERSION     DASRWS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667506"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFF72_09735"
     CDS             complement(<1..210)
                     /locus_tag="VFF72_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93488.1"
                     /translation="MLEVRNLVKEFGGLKAVDGVSFTLRAHTITGLIGPNGAGKTTLF
                     NTIAGVHRPNAGAISFLGRRIGGLPP"
     gene            complement(286..1557)
                     /locus_tag="VFF72_09740"
     CDS             complement(286..1557)
                     /locus_tag="VFF72_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93489.1"
                     /translation="MSKFTRLALVASTASLFCLVARPADAASCDTTIGGVMSLTGSLG
                     VLGQQIAKGAQLAVADLNDAGGVDRCTLKLSLLDDQTSPSVGVDAAKKLVDVQHVPAI
                     VGALSSGVSAAVLTSVAAPSKVVLISPASTSPTFTQLGKDGKTGGFFFRTTPSDALQG
                     VAMAKVALDAGLKKVAVLYLNNAYGQGLSQEFANAFGKLGGTVTQNVVYNPSQPSYRS
                     EVDKALSPKPDALFLVGYPGDGTTIAREWISSGGPQKWLLPDGLESQDFVNDVGAQYM
                     KNVHGTAAGSVKTPSLATFQGEFQKRFGSLPTQAYIPNAYDAVVLVGLAIDQARSADA
                     TAIRDGIRKVTDPKGEKIYAGAAELKKATTLLGRGKSIQYIGASGPLQFDKYGDINAP
                     MVVWTVDADGKVKSTGMVTVGQIDELREQAE"
     gene            complement(1831..>2633)
                     /locus_tag="VFF72_09745"
     CDS             complement(1831..>2633)
                     /locus_tag="VFF72_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV93490.1"
                     /translation="SAHMADDKMLREMTSDPKFRKALVTDGKTETGQAIVRALVKAGA
                     DIVWVGHAEPWKQFGGLDEITALPQVTLVPLDLTNGRSVTELAGEIGGKVDIVVNNAE
                     LHRAFGIGARHGTDVARAEMDINYFGLLRLAQEFGPAMKGRSADGVTHAAAWVNLLSI
                     YALSNYPPHGTFSASKAAAHSLAQCLRAEMRPAGIRVINVFPGPIDDEWNQNLPPPKV
                     TPAALASAIVKSLKEGIEDVYPGDAAQEWVERWRENPKVLERELAAGG"
BASE COUNT          394 a          896 c          918 g          425 t
ORIGIN      
        1 cggcggcagc ccgccaatgc gccggccgag aaacgagatc gcacccgcgt tcggccggtg
       61 cactccggcg atggtgttga agagcgtggt cttgccggca ccgttcggac cgatcaggcc
      121 ggtgatcgtg tgcgcgcgca gcgtgaacga gacgccgtcg accgccttca ggccgccgaa
      181 ctccttgacc aggttgcgaa cctcgagcac acgctgtccc gtttccggtc ggccgcgaac
      241 cgcccgggcg cctggaacgc gcctgcgggc ggaggcggca cgcgcctact cggcctgctc
      301 tcgcaattcg tcgatctgac cgaccgtgac catgccggtg gacttgacct tgccgtcggc
      361 gtcgaccgtc cagaccacca tcggcgcgtt gatgtcgccg tacttgtcga actgcagcgg
      421 gcccgaggcg ccgatgtact ggatcgactt gccccggccg agcagcgtcg tcgccttctt
      481 cagctccgcg gcgccggcgt agatcttctc gcccttcgga tcggtgacct tgcggatgcc
      541 gtcgcgtatc gccgtggcat cggcgctcct ggcctggtcg atcgccaggc cgaccagaac
      601 gaccgcgtca taggcgttgg gaatgtaggc ctgcgtgggc aagctgccga accgcttctg
      661 gaactcgccc tggaaggtcg cgagcgaagg cgtcttcacc gaacccgcgg cggtgccatg
      721 gacgttcttc atgtactggg cgccgacgtc gttgacgaaa tcctgcgact cgagcccgtc
      781 gggcagcagc catttctgcg ggccgcccga ggagatccat tcgcgcgcga tggtcgtgcc
      841 gtcgccggga tagccgacca ggaagagcgc gtcgggcttg gggctgagcg ccttgtcgac
      901 ttccgaccgg taggaaggtt ggctcgggtt gtagacgacg ttctgggtga cggtgccgcc
      961 gagcttgccg aacgcgttcg cgaactcctg gctcaatccc tgcccgtagg cgttgttcag
     1021 gtagagcacc gcaaccttct tcaggccggc gtcgagcgcc accttggcca tcgccacgcc
     1081 ttgcagcgcg tccgacggcg tggtgcggaa gaaaaagccg ccagtcttgc cgtccttgcc
     1141 gagctgggtg aaggtcggcg aggtcgacgc cggcgagatc agcacgacct tgctcggcgc
     1201 ggcgaccgaa gtcagcaccg ccgcgctgac gccgctcgac agggcgccga cgatggccgg
     1261 cacgtgctgc acgtcgacga gctttttcgc cgcgtcgaca ccgaccgacg ggcttgtctg
     1321 gtcgtcgagc agcgacagct tgagcgtgca gcgatcgacg ccgccggcgt cgttgagatc
     1381 ggcgacggcg agctgcgcgc ccttggcgat ctgctggccg agcaccccga gcgagccggt
     1441 gagcgacatg accccaccga tggtggtgtc gcacgatgcc gcgtcggcag ggcgggcgac
     1501 aagacagaac aggctcgcgg tcgaggcgac cagcgcgagc ctcgtgaatt tcgacatgac
     1561 gagatcctcc gggtgcgagc ggcggaggca gcgatccggg aggctgcaag accgtcgcag
     1621 ggacgccgaa ggcaatggag ggcgaggcgc gggcgccggg cagatcgccc tcgaaccgcc
     1681 ggggatgctc cgccggctcg gttcattgca tggcaatcga cgtgccgaaa gcgcggcggg
     1741 gtgaaaggcg cgccggggcg catttccagc acccgcggaa aaggcgcgcg ccgcgcaaga
     1801 ccgggcgctg cgaccgatcc cgaccggcgc tcagccgccc gctgccagct cccgctccag
     1861 caccttgggg ttctcgcgcc agcgctcgac ccactcctgc gccgcgtcgc cgggatagac
     1921 gtcctcgatg ccttccttca gcgacttgac gatggcgctc gccagcgccg ccggcgtcac
     1981 tttcggcggc ggcaggttct ggttccactc gtcgtcgatc ggccccggga agacgttgat
     2041 cacccggatg ccggcgggcc gcatctcggc gcgaaggcat tgcgcgagcg agtgcgccgc
     2101 cgccttcgag gccgagaagg tgccgtgcgg cggatagttc gaaagcgcgt agatcgagag
     2161 caggttcacc cacgcggcgg cgtgcgtcac gccgtcggcc gagcggccct tcatcgccgg
     2221 gccgaactcc tgcgccaggc gcaacaggcc gaagtagttg atgtccatct cggcccgggc
     2281 cacgtcggtg ccgtgccgcg cgccgatgcc gaaggcgcga tgcagctcgg cgttgttgac
     2341 gacgatgtcg accttgccgc cgatctcgcc ggcgagctcg gtcaccgagc ggccgttggt
     2401 gagatcgagc ggcaccagcg tgacctgcgg cagcgcggtg atctcgtcga ggccgccgaa
     2461 ctgcttccat ggctcggcgt ggccgaccca gacgatgtcg gcgccggcct tcaccagcgc
     2521 acgcacgatg gcctggcccg tctcggtctt gccgtcggtc accagcgcct tgcgaaactt
     2581 ggggtcgctg gtcatttcac gcagcatctt gtcgtcggcc atgtgggcac tcc
//
LOCUS       DASRWS010000521         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2483784, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000521 DASRWS010000000
VERSION     DASRWS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483784"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFF72_09750"
     CDS             <1..516
                     /locus_tag="VFF72_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZV93491.1"
                     /translation="QRDAFSDATGRSPGHVDGHQHVHQLPQIRDIVVDAEATTPAAQR
                     FAIRSTGRVIGPGHAFKRMVIEGTGGRGLLRMLRERRIRHNAALLGVYDFQDPDYRRL
                     VRGWLASAPADGGLVFCHPLATSPTTASVDAITAARRREADYLGSDAFAEDLATAGFK
                     VAAAWQSSSAG"
     gene            complement(476..895)
                     /locus_tag="VFF72_09755"
     CDS             complement(476..895)
                     /locus_tag="VFF72_09755"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZV93492.1"
                     /translation="MTAPPDDFAAPPPGGRNFLRYLAVGAVATAVHYAVLVTLVEAGH
                     VAPRFAAAAGAWIGAQVAFAGNARFTFGHAAGGVSAWWRFQLTALIGAAISFAVVAAG
                     VRLGLHYLLAQVVATAIAVFATYEINRRWSFATPRRP"
     gene            1042..2160
                     /gene="mnmA"
                     /locus_tag="VFF72_09760"
                     /pseudo
     CDS             1042..2160
                     /gene="mnmA"
                     /locus_tag="VFF72_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
     gene            complement(2358..2792)
                     /locus_tag="VFF72_09765"
     CDS             complement(2358..2792)
                     /locus_tag="VFF72_09765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV93493.1"
                     /translation="MTRDEPAQARVVPGKATPRGKYPHVRRAGDFLFVSGTSSRRADN
                     SIAGADADAMGTTLLDIAVQTRAVIENIRDILASVGADLADVVEVSTFLVDMNDFGAY
                     NAVYGEYFGENGPARTTVAVHQLPHPHLRIEIKAVAYKPRGA"
     gene            complement(2789..>2968)
                     /locus_tag="VFF72_09770"
     CDS             complement(2789..>2968)
                     /locus_tag="VFF72_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93494.1"
                     /translation="SIPDAAFATKTSDLVRPQGQGANRQPARGDHMTGSDAANVCRVA
                     ESIGVAHTSVKLRDR"
BASE COUNT          463 a         1050 c         1030 g          425 t
ORIGIN      
        1 cagcgcgacg ccttcagcga tgcgaccggt cgctcgcccg gccacgtcga cggccaccag
       61 cacgtgcacc agctgccgca gatccgcgac atcgtcgtcg atgccgaagc gacgacgccc
      121 gccgcgcagc gattcgcgat ccgcagcacc ggccgcgtca tcgggcccgg ccacgccttc
      181 aagcggatgg tgatcgaagg taccggcgga cggggcctgc tgcggatgct tcgggaacgg
      241 cgcatccgcc acaacgcggc gctgctcggc gtctacgact tccaggaccc cgactaccga
      301 cgtctcgtcc gcggctggct cgcctcggcg ccggccgacg gcggcctcgt cttctgccat
      361 ccgctcgcga cctcgccgac cacggccagc gtcgatgcga tcacggccgc gcgccggcgc
      421 gaagccgatt acctcggcag cgacgccttc gccgaagacc tcgcgacggc cggattcaag
      481 gtcgccgcgg cgtggcaaag ctccagcgcc ggttgatctc gtacgtggcg aagacggcga
      541 tcgccgtcgc caccacctgc gcgagaaggt agtgcaggcc gagacggacc ccggcggcca
      601 ccaccgcgaa gctgatcgcc gcgccgatca gcgcggtcaa ctggaagcgc caccatgccg
      661 agacgccgcc ggcagcgtgg ccgaacgtga accgggcgtt gccggcgaat gccacttgcg
      721 cgccgatcca tgcgccggca gcggccgcga aacgaggcgc gacgtgaccg gcttcgacga
      781 gggtgaccag cacggcgtag tgcacggcgg tcgcgacggc gccgacggcg agatagcgga
      841 gaaagttgcg gccgcccggg ggaggcgcgg cgaagtcgtc gggcggcgcc gtcatggaga
      901 gattgtcgga tgtcgtggcg gccctcgaca gcggccgccg ggccgcccac ggtcaccggc
      961 gagaccgccg cgtcgccgcc tcgcgcccgt catcgccctg gccgcgcatg gcgggcctgt
     1021 cgtcgctacg atgcccgcct catgatcgca gccaaggcct cctcccgccg gcagcgaatc
     1081 gtcgtcggcc tgtcgggcgg cgtcgactcg gccgttaccg catggctgct caagcgcgcc
     1141 ggccacgaag tggtcgccat cttcatgaag aactgggaag aggacgacga cgacgaattc
     1201 tgttcgtcgc gccaggactt tctcgatgcg gcgagcgtcg ccgacgtgct gggcatcgag
     1261 atcgagcacg tcaacttcgc cgccgactac aaggaccgcg tcttcgccga gttcctgcgc
     1321 gaataccgtg ccggccgcac gcccaatccc gacgtgcagt gcaacgccga gatcaagttc
     1381 aaggcgttcc tcgaccatgc gatgcgcctc ggcgcggacc ggatcgccac cggccactac
     1441 gcgcgcatcg gcgaggcggc cggcggccgc ttcactctgc tgaaaggccg ggacggtcgc
     1501 aaggaccaga gctacttcct gcatcgactc acgcaggggc agctggcgcg cacggtgttc
     1561 ccgatcggcg agctggaaaa gaccgaggtt cgccgcctcg ccgccgagat cggcctgccg
     1621 aatgcgaaga agaaggactc gacgggcatc tgcttcatcg gcgagcggcc ctttcgcgag
     1681 ttcctcaatc gcttcctggc gcacacgccc ggtcccgtcc gcgacgaccg cggccgcacc
     1741 ctcggagagc acgtggggct gtcgttctac acgctgggcc agcgcaaggg catcggcatc
     1801 ggcggcgtga aggagcgtgg cgcgcggcgc ggcggcggcg accacgcgcc ctggttcgtc
     1861 gccaggaagg acatcgccgc caacaccctc tacgtcgtgc caggccacga ccatccctgg
     1921 ctgcagagcc gctcgttgat cgctgccgac gcgagctgga ttgccggcca gccgccggcg
     1981 gcggggcgct atgccgcgaa gacgcgctat cggcaggccg acgcgacggc cgaggtcgtc
     2041 acggcggatg ctgccggcgc actcgcgctg cgcttcgatg cgccgcagtg ggcggtgacg
     2101 cccggccaat cagcggtcat ctacgacggc gacatctgcc tgggcggtgc cgtgatcgcc
     2161 cgcgcaggcc atggaaccga cgaagacggc gctgcgtcga cctcgaccac gtccccctcc
     2221 gccgcttcgc ccgacattca ggcgattcag gcgattcagg cgattcaggc gactcaggcg
     2281 actcgggcga ctcgggcgac tcaggcgact caggcgactc aggcgactca ggcgaccggg
     2341 accgggaccg ggacgactca ggcgccgcgc ggcttgtagg cgaccgcctt gatctcgatg
     2401 cgcaggtgcg gatgcggcag ctggtggacg gcgaccgtcg tgcgggccgg gccgttctcg
     2461 ccgaagtact cgccgtagac ggcgttgtag gcaccgaagt cgttcatgtc gacgagaaac
     2521 gtcgacacct cgacgacgtc ggcgagatcg gcaccgacgc ttgccaggat gtcgcggatg
     2581 ttctcgatga cagcgcgcgt ctgcaccgcg atgtcgagca gcgtcgtgcc catcgcgtcg
     2641 gcatcggcgc cggcgatgct gttgtcggcc cggcgcgagc tggtgccgct cacgaacagg
     2701 aaatcgcccg cacgccggac gtgcgggtac ttgccccgtg gcgtcgcctt gccgggcacg
     2761 acgcgcgcct gggcgggttc gtcgcgcgtc atcgatcgcg cagcttgacg ctggtgtgtg
     2821 cgacgccgat cgattcggcg acgcgacaca cgttggcggc atcggatccg gtcatgtggt
     2881 ctccccttgc tggttgtcga ttcgcgcctt gcccctgtgg acgaacgagg tcggacgtct
     2941 tcgtcgcgaa ggccgcatca ggaatcga
//
LOCUS       DASRWS010000522         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2796513, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000522 DASRWS010000000
VERSION     DASRWS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796513"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFF72_09775"
     CDS             <1..1007
                     /locus_tag="VFF72_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZV93495.1"
                     /translation="ILRGPHALRDDGLVDAARPPRIAGPDAVFDSARLGHDVRWGTPL
                     PARAELLGMLERQLDACIDAIPPSSDDAALYFHRLSLFHEDMHGEAFAWLRATLEWPA
                     PDGVAALRSLPEGGSLRIDACDVVLGRGEDEAGFSFDNEQPKTPTRVAAFEIDRAPLS
                     AGAFARFVDAGGYDDAALWPGAAGLWRAQAGRAQPLRWRRAAAGGWESRWFDRWLPLD
                     AGAPLIHVNAWEAEAYCRWAGRRLPTAAEWQAAARQPRFHWGGSVWEWTASAFEPYPG
                     FRAGPYSDYSRPWFGDHRELRGGSFATHPRLHDLAYRNFFTPERADVFAGFRTVAMPS
                     "
     gene            1026..1895
                     /locus_tag="VFF72_09780"
     CDS             1026..1895
                     /locus_tag="VFF72_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV93496.1"
                     /translation="MMFLRSLLAAGLIALGLGHAPAALAQADGVLRVSAIPDEAPTEL
                     QRKFKPLGEYLAKATGLKVEFVPVTDYAAVVEGLATNKLDLAWLGGFTYVQARIRTHG
                     GVVPLVQRAEDAKFTSRFIVPLNSTAKTLADLKGKTFAFGAPSSTSGSLMPRYFLLKA
                     GIQPERDFKSVAFSGAHDATVAFVAAGRAEAGVLNASVMDKLIEEHNPNAAKVRVLAI
                     TPPYFDYNWTVRGGMAPALRKKIADAFLALDPSKPGMKEIMDLQRASKFIPTQDSNYD
                     SIETAGRSAGLIK"
     gene            1916..2749
                     /locus_tag="VFF72_09785"
     CDS             1916..2749
                     /locus_tag="VFF72_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93497.1"
                     /translation="MSLELAAAGLVHANGHRALRDVTLSLAQGERAGIVGPSGAGKTT
                     LVRLLGTSLRPTEGQVGIAGVDPWRLSTRELRRLRSRIGVVHQNPPIPPRLRVVTAVL
                     AGRLGAWPTWRSLVSLLVPRDVEGARAALQPLALDDRVFDRCDRLSGGQLQRVGIARV
                     LYQRPDAILADEPVSALDPTLADEAVGALVAASEASGATLIASLHAVDLALKWFPRLI
                     GMKNGQVAFDRPTAAVTKSMLHDLYANEGRTLPVQGVEARDEEALRAANVLVLKRSGC
                     Q"
     gene            2822..>3593
                     /locus_tag="VFF72_09790"
     CDS             2822..>3593
                     /locus_tag="VFF72_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93498.1"
                     /translation="MSVVSPALAMPDARRRDPATRSRLTAIVVGLVILWPLLALSEFH
                     PSALFGSENMQVMANYLAAFLPPSTAPDFLALLGQSTLQTLAIATAGISLAFVVAAPL
                     AVLATRPLAASRIGPGPGRFADNAMRTSIRGVLVVLRSVPEVVWALVFVRALGLGPGA
                     GVLALAVTYGGMLGKVYIEILESGDTRSARALLEAGSGRLAALFYGLVPGAAQELASY
                     TVYRWECAVRASVVMGFVGAGGLGQLMDQSMKMLAGGET"
BASE COUNT          463 a         1299 c         1257 g          574 t
ORIGIN      
        1 ggatcctgcg cggaccgcac gcgctgcgcg acgacggcct cgtcgacgcg gcgcgtccgc
       61 cgcgcatcgc cgggcccgac gccgtcttcg actcggcgcg tctcggtcac gatgtgcgct
      121 ggggcacgcc gctgccggcc cgggccgagc tgctcgggat gctcgagcgg cagctcgacg
      181 cgtgcatcga cgcgattcca ccgtcgagcg acgacgcggc gctctatttc catcgcctgt
      241 cgctctttca cgaggacatg cacggcgagg ccttcgcgtg gctgcgcgcg acgctggagt
      301 ggccggcgcc cgacggggtg gccgcgctcc gctcgctgcc ggaaggcgga tcgttgcgca
      361 tcgatgcatg cgacgtcgtg ctcggccgtg gcgaagacga ggcggggttc tcgttcgaca
      421 acgagcagcc gaagaccccc acgcgcgtcg ctgcgttcga gatcgatcgg gcgccgcttt
      481 ctgccggtgc attcgcccgc ttcgtcgatg ccggcggcta cgacgacgca gcgttgtggc
      541 cgggcgccgc cggcctgtgg cgggcgcagg ccgggcgggc gcagccgctg cgatggcggc
      601 gagcggcagc cggcggctgg gaatcgcgct ggttcgatcg ctggctgccg ctcgacgcgg
      661 gcgctccgct catccacgtg aacgcctggg aagccgaggc ctactgccgc tgggccgggc
      721 gacggctgcc gactgcggcc gagtggcagg ccgccgcgcg ccagccacgc tttcattggg
      781 gcggcagcgt ctgggaatgg acggcaagcg ccttcgagcc ctatcccggc tttcgggccg
      841 gaccctacag cgactactcc cggccgtggt tcggcgatca tcgcgagttg cgcggcggct
      901 ctttcgccac ccacccccgg ctgcacgacc tggcctaccg caacttcttc actcccgaac
      961 gggccgacgt cttcgccggc tttcgcaccg tcgcaatgcc ctcctgaaca ggaaaaggaa
     1021 tccgcatgat gtttcttcgc tccctgctgg ccgccggcct gatcgccctc ggcctcggcc
     1081 acgcgccggc ggcgctggcg caagccgatg gtgtgcttcg cgtctcggcg attcccgatg
     1141 aggcaccgac cgagctgcag cgcaagttca agccgctggg cgaatatctc gccaaggcca
     1201 cgggcctgaa ggtcgagttc gtgccggtga ccgactacgc cgcggtcgtc gagggcctgg
     1261 cgacgaacaa gctcgacctc gcatggctcg gcggctttac ctacgtgcag gcgcgaatcc
     1321 gcacccacgg cggcgtcgtg ccgctggtgc agcgcgccga ggacgcgaag ttcacgagcc
     1381 gtttcatcgt gccgctgaac agcaccgcga agacgctggc cgacctgaag ggcaagacct
     1441 tcgccttcgg ggcgccctcg tcgacgtcgg gcagcctgat gccgcgctat ttcctgctca
     1501 aggccggcat ccagccggag cgcgacttca agtcggtcgc cttctcgggc gcccacgatg
     1561 cgacggttgc cttcgtcgcc gccggccgcg ccgaggccgg cgttctcaac gcctcggtga
     1621 tggacaagct gatcgaggag cacaacccga acgccgccaa ggttcgcgtc ctcgccatca
     1681 cgccgccgta cttcgactac aactggacgg tgcggggcgg aatggcgccg gcgctcagga
     1741 agaagatcgc cgatgcgttt ctcgccctcg acccgagcaa gcccgggatg aaggagatca
     1801 tggacctgca gcgtgcctcg aaattcatcc cgacccagga ctcgaactac gacagcatcg
     1861 agaccgcggg ccgctccgcc ggcctgatca agtaggccag gacttcgctt cgccgatgag
     1921 tctcgagctc gccgccgccg gcctcgttca tgcgaacggg caccgcgccc tgcgcgacgt
     1981 cacgctgtcg ctggcgcaag gcgagcgggc cggcatcgtc ggcccgtcgg gcgccggcaa
     2041 gacgacgctg gtccgcctgc tcggcacgtc gctgcggccg acggaagggc aggtcggcat
     2101 cgccggggtc gatccgtggc ggctgtcgac gcgcgagctg cgccgcttgc gctcccggat
     2161 cggcgtcgtc caccagaatc cgccgatccc gccgcggctt cgcgtcgtca ccgcggtgct
     2221 cgccggccgg ctcggcgcgt ggccgacctg gcggtcgctc gtctcgctgc tcgttccgcg
     2281 cgacgtcgaa ggcgcccgcg ccgcgttgca gccgctggcg ctcgacgacc gggtcttcga
     2341 ccgctgcgac cgtctctccg gcggccagtt gcagcgcgtc ggcatcgccc gggttctcta
     2401 ccagcgaccc gacgcgatcc tcgccgacga gccggtctcg gcgctcgacc cgacgctcgc
     2461 cgacgaagcc gtcggcgccc tcgtcgcggc gagcgaagcg agtggcgcga cgctgatcgc
     2521 ctcgctgcat gccgtcgacc tggcgctcaa atggtttccg cgcctcatcg gcatgaagaa
     2581 cggccaggtt gccttcgacc ggccgaccgc ggcggtgacg aagtcgatgc tgcacgatct
     2641 ctacgccaac gaagggcgga cgctgccggt ccagggcgtc gaggcgcgcg acgaggaggc
     2701 gctgcgtgcc gccaacgtcc tcgtgctgaa gcgatcggga tgccaatgac gcgacgtgcg
     2761 cgcctcgccg cgctgtcgct gccggcgtgc gcctgagcgt tcgcgggacc cggtcgcaac
     2821 gatgagcgtc gtttcccctg cgctggcgat gcccgatgcg cgtcgccgcg atccggcgac
     2881 gcggagccgg ctgacggcga tcgtcgtcgg gctcgtgatc ctctggccgc tcctggcgct
     2941 gagcgagttc cacccgtcgg cgctgttcgg cagcgagaac atgcaggtga tggcgaacta
     3001 cctcgccgcc ttcctgccgc cgtcgacggc gcccgatttc ctcgccctgc tggggcagtc
     3061 gaccttgcag acgctggcga tcgccaccgc cgggatctcg ctcgcgttcg tcgtcgcggc
     3121 gccgctggcg gtgctggcga cgcgtccgct cgcggcatcg cgcatcggcc cggggcccgg
     3181 ccgcttcgcc gacaacgcga tgcgcacgag cattcgcggc gtcctcgtcg tgctgcgctc
     3241 ggtgcccgaa gtcgtgtggg cgctcgtctt cgtccgcgcc ctcggcctcg gccccggtgc
     3301 cggcgtgctc gccctggcgg tcacctacgg cggcatgctc ggcaaggtct acatcgagat
     3361 cctcgagtcg ggcgacacgc gctcggcgcg agcgctgctc gaagccggct ccggccgtct
     3421 cgcggccttg ttttacgggc tcgttcccgg cgccgcgcag gagcttgcgt cgtacaccgt
     3481 gtatcgctgg gagtgcgcgg tgcgcgcctc ggtggtgatg ggattcgtcg gcgcaggcgg
     3541 cctcgggcag ctgatggatc agtcgatgaa gatgctggcc ggcggcgaga cga
//
LOCUS       DASRWS010000523         5726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1081805, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000523 DASRWS010000000
VERSION     DASRWS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081805"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1019
                     /locus_tag="VFF72_09795"
     CDS             285..1019
                     /locus_tag="VFF72_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZV93499.1"
                     /translation="MTTSARLPPDSPRALAFDVFGTVVDWHEGVAREAAALAARHGVE
                     GDWAAFARAWRAGYIPAMDKVRSGELPWLRIDVLHRRILDDIIEAHGLGCLGEDERAR
                     LNLAWHRLPPWPDAVPGLLRLKRRFTLTTLSNGNFSLLTDMARHGGLPWDCIVSAELF
                     GRYKPDPETYLGCARLLDVAPAELMMVACHVSDLRAARASGLRTAYVRRPREAGAVTP
                     APQFDPAEFDLVADDFGQLADLLAAP"
     gene            1184..2566
                     /locus_tag="VFF72_09800"
     CDS             1184..2566
                     /locus_tag="VFF72_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HZV93500.1"
                     /translation="MRQKTAVRGVATLVVVGAAWLGATAWSGQQARARYGAELGRIEA
                     RFPFMRVVDRKYDKGFFSSTATTSFQFGCPAGAGAPPATLTLTSVVHHGPIAGRTLAA
                     AVVDSQVMLAGAAQPVAAAFAGAAPLTVRTVVGFTGRDRSTFDSPPAHASVGEHAVVA
                     WQGLSGTVDSGQHGRSMTYRVRSPGLVVSDPASHAVMRIGAMTVSGDGEATSDSGLIM
                     VGKVNGTLDAVEVNDGKAVEPMKIMLTGLHFATQTAINGELIDGTGTFGGTGSVGDVR
                     IDRFEMESSMHRLHAPTYEHLMQSTSAEMYRCDIADKDRLAGLLSMQKAMAGDLMAML
                     RYGPEISLDKFVVESGGRTAEVAYAVGVDGVTDADARLPPAAVLMAHAHASASARVPI
                     DWLRRLSETTAARLQGKGPDPANLDLMIDQAQAQGYLVRDSDYVTSKAEFAGGVLKVN
                     GRELGPKPRR"
     gene            complement(2591..3529)
                     /locus_tag="VFF72_09805"
     CDS             complement(2591..3529)
                     /locus_tag="VFF72_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HZV93501.1"
                     /translation="MNTADPVLEVLPRDLSAYRAGNTGIDYVHRFDSGRPGPDVMINA
                     LTHGNELCGMVAVCHLLDSDVRPKIGTLTLSFANVAAYETFDAARPFESRLITHNLNR
                     IWSAAQLEGSDDSVELRRAREMRPAVAAAQHILDIHSTSQDVVPFWVYPAFERNAAVA
                     RSLPRPAVHLVMPAGLGSGTPLIQHGAHGDPASRAGAALVVECGQHFRQASADLAVDV
                     ALDFLGHFGLVDRPVPPRAAPPRRFELLRTHVIQNDSFAFTRPVIGFETFAKDELIAT
                     DGPLEIRAPCDDCTIFMPARVAIVGREGVYLTRPLH"
     gene            complement(3615..4034)
                     /gene="trxC"
                     /locus_tag="VFF72_09810"
     CDS             complement(3615..4034)
                     /gene="trxC"
                     /locus_tag="VFF72_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HZV93502.1"
                     /translation="MLIVCPHCLTTNRVPDARLAESPTCGHCQQELLGAAPVPLDDAS
                     FDKVVAKTELPVVVDFWADWCGPCRAMGPQYAKAAAELKGKALFAKVDSDAAPRTSAR
                     FAVRSIPTLLMLRGGREVKRQAGAIQAAQIVDWVSHG"
     gene            complement(4051..5094)
                     /locus_tag="VFF72_09815"
     CDS             complement(4051..5094)
                     /locus_tag="VFF72_09815"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZV93503.1"
                     /translation="MYVRNAWYVACTVDEIGHGKPLGRTICGERMVIFRAEGGQVSVL
                     EDFCPHRGAPLSLGAIREDGRLVCGYHGLVMGGDGRCVSMPGQRVRPTIRSYPVLERY
                     GFIWVWPGDKSLADAAALHPLPWAESPDWAYGGGLFNVKCDYRLMIDNLMDLTHETYV
                     HATSIGQPEIEEAAPSTRVEGDSAITSRFMHDIPAPPFWRAALRGNHLADDVPVDRWQ
                     ICRFTPPSHVMIEVGVAHAGHGGYDAPAERKVSSIVVDFITPETDTSIWYFWGMARNF
                     NVEDKALTETIRSGQEKIFTEDVAILERQQLNLSEQSSRHLMRLNIDMGGFESRRILE
                     RLVAEEREDLLSR"
     gene            complement(5162..>5726)
                     /locus_tag="VFF72_09820"
     CDS             complement(5162..>5726)
                     /locus_tag="VFF72_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93504.1"
                     /translation="LVIDRDNNRLYELDDGHLNGDGSWNAACGAVFHLDSDTVRPTAQ
                     PGWTSADAAGLPIFPGLARWEEAALGPGGIRHALRFTVQHTRRAYVPPASHWASTSTS
                     GSLPPMGMRVRLKASYAIPAGFSVETRALLTAMQTYGMLVADNGSNWFVSGAPDDRWD
                     NDTLVGELRQVTGSSFEVVRMDGLVAG"
BASE COUNT          838 a         1989 c         2035 g          864 t
ORIGIN      
        1 accttctggc ccggctggcg aaacggctcg ccgcgccgtc gaatcggaga agagcgcgca
       61 ttcgtatccc ggcgatgtaa tggattacat caaggcgagg tactttggca ccttcggggc
      121 acccgcggtc agcgggtcaa cccgatgatc gagcccgcgc cccggcgcgg ctgcccaggc
      181 atcatcgccg ccgtgggccg tgtcgcgacg cctcgcgccc ggtccgcgcg aaccaccgca
      241 cctcacgccg ccactttcgc gccaactgat cgaagggcaa cgccatgacg acctccgccc
      301 gccttccccc ggactcgccg cgagccctcg ccttcgacgt gttcggcacc gtcgtcgact
      361 ggcacgaagg cgtcgcgcgc gaggccgccg cgctcgcggc acgccacggc gtcgaaggcg
      421 actgggccgc cttcgcgcgc gcctggcgcg ccggctacat cccggccatg gacaaggtgc
      481 gcagcggcga gctgccgtgg ctgcgcatcg acgtgctgca ccgccggatc ctcgacgaca
      541 tcatcgaagc gcacggactg ggctgcctcg gcgaggacga gcgggcgcga ctcaacctcg
      601 cctggcatcg cctgccgccc tggcccgatg cagtgccggg cttgcttcgg ttgaagcgtc
      661 gcttcacgct gacgacgctc tcgaacggca atttctccct cctcaccgac atggcccggc
      721 acggcggcct gccctgggat tgcatcgtct cggccgagtt gttcggccgc tacaagcccg
      781 atcccgagac ctatctggga tgcgcccgcc tgctcgacgt ggcaccggcc gagctgatga
      841 tggtcgcctg ccatgtctcc gacctgcgag cggcacgcgc cagcggcttg cgcacggcct
      901 atgtccggcg gccgcgcgag gccggcgcgg tgacgccggc gccgcagttc gatcccgccg
      961 agttcgatct cgtcgccgac gacttcggcc agctcgccga cctgctcgcc gcgccctgac
     1021 gccagctgtc gccgtcggcg cggggtggca tgcgccggcg gtgcgggcgc gacacgacag
     1081 gcggcgcgcg acgcgcttgc gaattcgccg gcccggcgcg aacatgccgg tcgcgcgcga
     1141 gcaccgcgct tgacgacggt gcagcgctga accgggaggt cacatgaggc agaagaccgc
     1201 cgtccgcggc gtcgcaacac tcgttgtcgt gggagcggcc tggctcggcg ccacggcgtg
     1261 gtccggacag caggcccggg cccgctacgg cgccgagctc ggtcggatcg aggcgcggtt
     1321 tccgttcatg cgggtcgtcg atcggaaata cgacaagggg ttcttctcgt cgaccgccac
     1381 gacctcgttc cagttcggtt gccccgccgg tgccggagcg ccgccggcga cgctgacgct
     1441 caccagcgtc gtgcaccacg gtccgatcgc cggtcgcacg ctggccgcgg cggtggtcga
     1501 ttcgcaggtc atgctggccg gcgcggcgca gcccgtggcc gccgccttcg ccggcgccgc
     1561 tccactgacc gtgcgaacgg tggtcggctt cacgggccgc gaccgcagca cgttcgacag
     1621 cccgccggcg cacgcgtcgg tgggcgagca cgccgtcgtc gcgtggcagg gcctgagcgg
     1681 cacggtcgac tcgggccagc acgggcgctc gatgacgtac cgcgtccgca gtcccgggct
     1741 cgtcgtcagc gatccggcca gccacgccgt catgcgcatc ggcgcgatga ccgtttcagg
     1801 cgatggcgag gccaccagcg acagcggcct gatcatggtc ggcaaggtga acggcacgct
     1861 cgacgccgtc gaggtcaacg acggcaaggc cgtcgagccc atgaagatca tgctcacggg
     1921 gctgcacttc gcgacccaga cggcgatcaa tggcgagctg atcgacggca cgggcacgtt
     1981 cggtggcacc ggcagcgtcg gcgacgtcag gatcgatcgt ttcgagatgg aaagctcgat
     2041 gcaccggctg catgcgccga cctacgagca tctgatgcag tcgacctcgg ccgagatgta
     2101 ccgctgcgac atcgccgaca aggatcggct ggccgggctg ctctcgatgc agaaggcgat
     2161 ggcgggcgac ctgatggcga tgctgcgcta cggccccgag atttcgctcg acaagttcgt
     2221 cgtcgaatcg ggtggccgca ctgcagaggt cgcctacgcc gtcggcgtcg atggcgttac
     2281 cgatgccgac gcccggttgc cgccggccgc ggtgctgatg gcgcacgcgc atgcgagtgc
     2341 gtcggcccgg gtgccgatcg actggttgcg caggctctcc gaaacgacgg cggcacgcct
     2401 gcagggcaag gggcccgacc cggccaacct cgacctgatg atcgaccagg cacaggcaca
     2461 gggctacctc gtgcgcgaca gcgactacgt gacgagcaag gccgagtttg cgggcggcgt
     2521 gttgaaggtc aacggccgcg agctcgggcc gaagccgcgc cgatagtcgc ccggcgtcga
     2581 gtgctgccgc tcagtgcagc ggccgggtca gatagacgcc ctcgcggccg acgatggcca
     2641 cgcgcgccgg catgaagatc gtgcagtcgt cgcacggggc gcggatctcg agcggcccgt
     2701 cggtggcgat cagctcgtcc ttcgcgaagg tctcgaagcc gatcaccgga cgcgtgaagg
     2761 cgaagctgtc gttctggatc acgtgcgtgc gcagcagctc gaagcggcgc ggtggcgcgg
     2821 cgcgtggcgg caccggccgg tcgacgaggc cgaagtggcc gaggaagtcg agcgcgacgt
     2881 cgaccgcgag gtcggcgctc gcctgcctga aatgctgtcc gcactcgacc accagcgccg
     2941 caccggcgcg gctcgcggga tcgccgtgcg cgccgtgctg gatcagcggc gtgcccgagc
     3001 cgaggccggc cggcatgacc agatgcaccg cagggcgcgg cagcgaccgt gccacggcgg
     3061 cattgcgctc gaaggcggga tagacccaga acggcacgac gtcctggctg gtcgagtgga
     3121 tgtcgaggat gtgctgcgct gccgccaccg ccgggcgcat ctcgcgcgcc cggcgcaact
     3181 cgacgctgtc gtcgctgccc tcgagttgcg ccgccgacca gatgcggttc aggttgtgcg
     3241 tgatcagccg gctctcgaac ggccgcgcgg catcgaaggt ctcgtaggcg gcgacgttgg
     3301 cgaagctgag cgtgagggtg ccgatcttcg gccgcacgtc gctgtcgagc agatggcaca
     3361 ccgcgaccat gccgcacagc tcgttgccgt gcgtgagggc attgatcatg acgtcgggcc
     3421 cgggccgacc cgagtcgaac cggtgcacgt agtcgatgcc ggtgttgccg gcccgatagg
     3481 ccgacaggtc gcggggcagg acttcgagga cggggtcggc ggtgttcatc gccggattgt
     3541 gccgccggtc ttgcgaagct caggcactgc tcagcgctgg cgacggagct ttgcgaaggc
     3601 gcgcggcctc gatctcagcc gtggctcacc caatcgacga tctgcgccgc ctggatcgcg
     3661 ccggcctggc gcttgacctc gcggccgccg cgcagcatca gcaatgtcgg gatgctgcgc
     3721 acggcgaacc gcgccgaagt gcgcggcgcg gcgtcgctgt cgaccttggc gaacagcgcc
     3781 ttgcctttca gctcggccgc ggccttggca tattgcggcc ccatcgcgcg gcacggcccg
     3841 caccagtcgg cccagaaatc gacgacgacc ggcagctccg tcttcgccac caccttgtcg
     3901 aagctggcgt cgtcgagcgg caccggcgcg gcgccgagca gttcctgctg gcaatgcccg
     3961 caggtcggcg attcggcgag ccgcgcgtcg ggcacccggt tggtcgtgag gcagtgcggg
     4021 cagacgatga gcatggcgag gctccggcgt tcagcggctg agcaggtctt cgcgctcctc
     4081 ggcgacgagt cgctcgagga tgcgtcgcga ttcgaagccg cccatgtcga tgttgaggcg
     4141 catcaggtgc cggctcgact gctccgacag gttgagctgc tggcgctcga ggatcgcgac
     4201 gtcctcggtg aagatcttct cctggccgga gcggatcgtc tcggtgagcg ccttgtcctc
     4261 gacgttgaag ttgcgggcca tgccccagaa gtaccagatc gaggtgtcgg tctcgggcgt
     4321 gatgaagtcg acgacgatgc tcgagacctt gcgctcggcc ggtgcgtcgt agccgccatg
     4381 cccggcgtgg gcgacgccga cctcgatcat gacgtggctg ggcggcgtga agcggcagat
     4441 ctgccagcgg tcgaccggta cgtcgtcggc cagatggttg ccgcgcagcg ccgcgcgcca
     4501 gaacggcggc gccgggatgt cgtgcatgaa gcggctggtg atggcgctgt cgccctcgac
     4561 gcgcgtcgag ggcgccgcct cctcgatctc gggctggccg atgctggtcg cgtgaacgta
     4621 ggtttcgtgc gtcagatcca tcaggttgtc gatcatcagg cggtagtcgc acttgacgtt
     4681 gaagaggccg ccgccgtacg cccaatcggg gctctcggcc cagggcagcg gatgcagcgc
     4741 cgcggcgtcg gcgaggctct tgtctcccgg ccacacccag atgaagccgt agcgctcgag
     4801 caccggatac gagcggatcg tcggccgcac gcgctgcccc ggcatgctga cgcagcggcc
     4861 gtcgcctccc atcacgaggc cgtggtagcc gcagacgagc ctgccgtcct cgcggatcgc
     4921 accgagcgac agtggcgcgc cgcggtgcgg gcagaaatcc tcgagcaccg agacctggcc
     4981 gccctcggcg cgaaagatca ccatccgctc gccgcagatc gtccgtccga gcggcttgcc
     5041 gtggccgatc tcgtcgaccg tgcaggcgac ataccaggcg ttgcggacgt acatggggtt
     5101 ctccgggcag cgcgcggcgg cgacggcgcc ggcatccagc cgttatagcg ccaaccgcgt
     5161 ttcagccggc gaccaggcca tccatgcgaa cgacctcgaa cgagctcccc gtcacctggc
     5221 gcaactcgcc gaccagcgtg tcgttgtccc agcggtcgtc gggcgctccg ctgacgaacc
     5281 agttcgagcc gttgtcggcg acgagcatgc cgtaggtctg catcgccgtc agcaaggcgc
     5341 gcgtctcgac gctgaagcct gccggaatcg catagctcgc cttcaggcgc acccgcatcc
     5401 ccatcggcgg caggctaccg ctcgtgctcg tcgaagccca gtgcgacgcc ggcggcacgt
     5461 aggcgcggcg cgtgtgctgc accgtgaagc gcagcgcatg acggatgccg ccgggcccga
     5521 gcgcggcttc ttcccaacgc gccaggccgg gaaagatcgg caggccggcg gcatcggcac
     5581 tggtccagcc gggctgggcc gtcggtcgca ccgtatcgct gtcgaggtgg aacaccgcgc
     5641 cgcacgcagc gttccacgag ccgtcgccat tgaggtggcc gtcgtcgagc tcgtagagcc
     5701 ggttgttgtc gcggtcgatg acgagg
//
LOCUS       DASRWS010000524         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4230586, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000524 DASRWS010000000
VERSION     DASRWS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4230586"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFF72_09825"
     CDS             complement(<1..364)
                     /locus_tag="VFF72_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93505.1"
                     /translation="MTLDRRLLSCTLASSVLLVACAATPITPAPAPAAPARSVAEPMR
                     APGGAPAATAASARASTSAAPSVAIVDPKDLVPPAPARFEDAVARAGRQLFMQAQSVL
                     GPAPRLLVVDPLIDANTGG"
     gene            complement(406..2631)
                     /locus_tag="VFF72_09830"
     CDS             complement(406..2631)
                     /locus_tag="VFF72_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93506.1"
                     /translation="MKRFQSPGSRRALARMAFALSAAALVSCGGGSTGSDQASGPEQT
                     YLSVQASDPDGDTLHYRWRVTAGTIVDVDSRQTVWRLPDGPGLHFAYVAVSDGKGGWT
                     EQQYGVSSDFLATPAPATAPITRAAPAVADVDGSMNRLRVSSADSTLFAAAAGATPQA
                     RIVYLPGVQIQVVATATGAVAFAGRTDLAGEVDLPKLPAGSSYQIMCSTQDGTPLALC
                     GSMTVSSLAAVRLVSPPLASARNLRLFGHVALADGGVCGHENPYLGVLTAATVQLEQS
                     DGAPIGSPATVNRYGDYELDAAVPVNASLQAQIACEGYSATVPVPASSDPAGYVSGTP
                     VELSYVIANSRPAIVKVVANGPDGNVRGDVVVPIDDSPSAAIPGADHFLVYKGADTKL
                     SACQYYVSLGAAQSCDAQGNMVAPISFNDWLTKNGFGGPGDVSALYVNQRDLNLVRHM
                     VATRSPSGSIAFSVCNSPGPKGLSQAEIDRVIDDNASGFNRVACVTMEYSSVPGVNGG
                     QPFTQFYTFDAAGQLLLSINLDTRGEKYMPGSCVACHGGLAYNGKFPEQAGASPFLGS
                     RFLPFDTGNYLFSSAASLTEPDQEEAFYQLNQLVQATELSNTSPTSTLIQGWYASGHI
                     LDKSYVPPAWLAADAQPATAGAADFYRHVVAISCRTCHVALNPGQYDWNSLILSPARV
                     SRYYCGGTADVATNATMPNALISNDRLIQEIAADPGLTDLVTQFMGCAAPLPDPVYPK
                     R"
     gene            complement(2769..4607)
                     /locus_tag="VFF72_09835"
     CDS             complement(2769..4607)
                     /locus_tag="VFF72_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZV93507.1"
                     /translation="MDSDEDDRTVIRPPGARTTSTTPGAAFERTAAATTPHATGGFAP
                     TTGSPATTPPVAAPAGGEQHALTLPAGTRLAEFEITKTIGEGGFGIVYLASDHSLQRN
                     VALKEYMPSSIASRVGATEIRPRSERHQETFDAGLKSFINEARLLAQFDHPSLLKVHR
                     FWEANGTAYMVMPFLQGTTLRDTVRALPEPPGEAWIIGFLAPLLDALAMLHAAQCFHR
                     DIAPDNILLLADSGRPLLLDFGAARRVIGETTQALTAILKPGYAPVEQYAEIPGLKQG
                     AWTDIYALAAVVHWTITGKTPTASVGRLFNDTHVPLAERVAGRYSAAFLEAIDRALVV
                     MPDKRTQTVEAFRDEIMAGGTSGILAPATWPSPGATPVALDSRSRQHPTTPGSRPGVT
                     EPPPLTAAATVRLPPVGATDSAVPAPRRRLGWIAAAVVLAAIAAGVAFFLRPSPPTSD
                     ASRETAVAPASTNSTTNAREGSATTSPSPSPAAAASAVVLPLALPPAPQATETAAPAP
                     SPGETGGESATAVPVAPAAEKTPEPARASRPRIDEKREVRAAEPVERTAKKAARTESR
                     DEPVPAATPAKPANDNQAECARLFQRLSLGDTDPSIAERVRTLRCR"
     gene            4814..5050
                     /locus_tag="VFF72_09840"
     CDS             4814..5050
                     /locus_tag="VFF72_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93508.1"
                     /translation="MKTYDIEVQHLKSARHDKGLIELEVDALVLPRPARGEEERASSL
                     RLPVEHARSLLILLRQQLGELDRLNPRSRRSGRA"
BASE COUNT          742 a         1694 c         1963 g          799 t
ORIGIN      
        1 ggccgccggt gttcgcgtcg atcagcgggt cgacgacgag caggcggggc gccggcccga
       61 gcaccgactg ggcctgcatg aacagctggc gcccggcgcg cgcgacggca tcctcgaatc
      121 tggcgggcgc cggaggtacg agatccttcg ggtcgacgat ggcgacggac ggcgcggcgg
      181 atgtgctcgc ccgtgcgctc gccgcggtgg cggcgggtgc gccccccggg gcgcgcatcg
      241 gctccgcgac gctgcgcgcc ggcgcggccg gcgccggtgc gggcgtgatc ggcgtggcgg
      301 cacaggccac cagcagcacc gacgaggcga gcgtgcagga aagcagtcgg cgatcaaggg
      361 tcatgatgaa gcggttctcg caaaggggat cggagcgtcg gcgcgtcagc gcttcggata
      421 gaccgggtcg ggcaacggcg ccgcacagcc catgaactgc gtgacgaggt cggtcaggcc
      481 gggatcggcc gcgatctcct ggatcaggcg atcgttggag atgagggcgt tgggcatggt
      541 ggcgttggtg gcgacatcgg ccgtgccgcc gcagtaatac ctcgacacgc gggccggcga
      601 caggatgagc gaattccagt cgtactgtcc cgggttgagc gcgacgtggc acgtgcggca
      661 cgagatggcg acgacatggc gatagaagtc ggccgcgccc gcggttgccg gctgggcatc
      721 ggcggcgagc caggcgggcg gcacgtagct cttgtcgagg atgtggcccg acgcgtacca
      781 gccctggatc aacgtgctcg tcggcgaggt gttgctcaat tcggtggcct gcaccagctg
      841 gttgagctgg tagaaggcct cttcctggtc gggctcggtc agcgaggcgg cgctcgagaa
      901 caagtagttg ccggtgtcga aggggaggaa gcgcgagccg aggaagggcg aggcaccggc
      961 ctgctccggg aacttgccgt tgtaggccag gccgccatgg caggcgacgc acgatcccgg
     1021 catgtacttc tcgccgcgcg tgtcgagatt gatcgacagg agcagctggc cggccgcatc
     1081 gaacgtgtag aactgggtga acggctgtcc gccgttgacg ccgggcaccg atgaatactc
     1141 catcgtgacg caggcgaccc ggttgaaccc gctcgcgttg tcgtcgatca cgcgatcgat
     1201 ctcggcttgc gacaggccct tgggcccggg gctgttgcag accgaaaagg cgatcgaccc
     1261 ggatggcgaa cgcgtcgcca ccatgtggcg cacgagattg aggtcgcgct ggttgacgta
     1321 gagcgccgag acgtcgccgg ggccgccgaa gccgttcttc gtcagccaat cgttgaagga
     1381 gatcggcgcc accatgttgc cctgcgcatc gcagctctgc gcggcgccca gcgagacgta
     1441 gtactgacag gcgctcagct tggtgtcggc gcccttgtag acgagaaagt ggtcggcgcc
     1501 cggaatggcg gccgatggcg aatcgtcgat cggaacgacg acgtcgccgc ggacgttgcc
     1561 gtcgggcccg ttggccacca ccttgacgat ggcagggcgg ctgttggcga tgacgtacga
     1621 cagctcgacc ggcgtgccgc tcacatagcc cgccgggtcg ctcgatgccg gaacgggcac
     1681 cgtcgccgaa tagccttcac aggcgatttg cgcctgcagc gaggcgttca ccggcaccgc
     1741 cgcgtcgagc tcgtagtcgc cgtagcggtt gacggttgcc ggcgagccga tcggcgcgcc
     1801 gtcggactgt tcgagctgca cggtggcggc ggtaaggacg ccaagatacg ggttttcgtg
     1861 gccgcagacg ccgccgtcgg cgagcgcgac gtgaccgaac agccgcaggt tgcgcgcact
     1921 ggcgagaggg ggcgagacga gtcgcaccgc cgcaaggctg gagacggtca tcgagccgca
     1981 gagggcgagc ggcgtgccgt cctgggtcga gcacatgatc tggtagctgc tgcctgccgg
     2041 cagcttcgga agatcgacct cgccggcgag atcggtgcgg ccggcgaagg cgacggcgcc
     2101 ggtggccgtc gccacgacct ggatctgcac gccgggcagg tagacgatgc gggcctgcgg
     2161 cgttgcgccg gcggccgcgg cgaagagcgt gctgtcggcc gaggagacgc gcagccggtt
     2221 catcgagccg tcgacatcgg ccactgccgg ggcagcgcgc gtgatgggcg ctgtggccgg
     2281 cgcgggcgtg gcgaggaaat cggacgacac accgtactgc tgctcggtcc agccgccctt
     2341 gccgtcggat accgcgacgt aggcgaagtg caggcccggg ccgtcgggaa gcctccagac
     2401 ggtctgccgg ctgtcaacat cgacgatggt gccggcggtg acgcgccatc gatagtgcag
     2461 cgtgtcgccg tcggggtcgc tcgcctgcac gctcaggtag gtctgctcag ggccgctggc
     2521 ctgatcggag cccgtcgatc cgccgccgca cgacaccagc gccgctgccg acagcgcgaa
     2581 cgccatccgg gcgagggcgc gccgagatcc cggcgattgg aaccgcttca tcctcttgat
     2641 cctcttgtcg acccggggcc gttggctggc cgcgtcctgg agcgcgcgtg gatgcgagcg
     2701 cttgtgacga ggcgcagcga tcgttgacga aggcgtcgct gccgtcaacc ccccggtttt
     2761 gccgaggcct atcgacaacg aagcgtgcga acgcgctcgg cgatgctcgg gtcggtgtcg
     2821 cccagcgaca ggcgctggaa cagccgcgcg cactcggcct ggttgtcgtt ggccggcttg
     2881 gcgggcgttg cggccggcac cggctcgtcg cggctttcgg tgcgtgccgc cttcttcgcg
     2941 gtccgctcga ccggctcggc ggcgcggact tcgcgcttct cgtcgatgcg cggtctggat
     3001 gcccgggccg gttcgggggt cttctccgcg gcgggagcca ccggcaccgc ggttgccgat
     3061 tcaccgcccg tctcccccgg cgagggtgcc ggtgcggccg tttccgtcgc ttgcggcgcc
     3121 ggtgggagtg ccaagggcag gaccaccgcc gacgccgcgg cggccggact cggcgaggga
     3181 cttgtcgtgg cgctgccttc gcgagcgttc gtcgtcgagt tcgtcgacgc cggcgccacg
     3241 gcggtttcgc gggacgcatc gctggtcgga ggcgacggtc gcaggaagaa ggcgacgccc
     3301 gcggcgatcg ccgccagcac gacggccgcg gcgatccagc cgagccggcg tcgcggcgcg
     3361 ggtaccgccg agtcggtggc accgactggc ggcaaccgca ccgtcgctgc cgcggtgagc
     3421 gggggcggtt cggtgacgcc cgggcgcgac ccgggcgtgg tcggatgctg ccggctacgc
     3481 gaatcgagcg caaccggcgt cgcgccgggc gacggccacg tggcgggcgc caggatgccc
     3541 gacgtgccgc cagccatgat ctcgtcgcga aacgcctcga cggtctgcgt ccgcttgtcg
     3601 ggcatcacga ccagggcgcg gtcgatggct tcgagaaagg cagcgctgta ccggccggcg
     3661 acgcgttcgg cgagcggcac gtgcgtgtcg ttgaacagac gaccgaccga tgccgtcggc
     3721 gtcttgcccg tgatcgtcca gtgaacgacc gcggcaagcg cgtagatgtc ggtccaggcg
     3781 ccttgcttca gcccggggat ctcggcgtac tgctccaccg gcgcatagcc cggcttgagg
     3841 atggcggtga gcgcctgcgt ggtctcgccg atgacgcggc gcgcggcgcc gaaatcgagc
     3901 agcagcggcc ggccgctgtc ggccagcagc aggatgttgt cgggcgcgat gtcgcggtgg
     3961 aagcactggg cggcatgcag catcgccagc gcgtcgagca ggggcgcgag aaagccgatg
     4021 atccacgctt cgcccggcgg ttcgggcagt gcgcgcaccg tgtcgcgcag ggtcgtgccc
     4081 tgaaggaagg gcatcaccat gtaggccgtg ccgttggctt cccagaagcg gtgtaccttg
     4141 agcagcgaag ggtggtcgaa ctgcgcgagc agccgcgcct cgttgatgaa gctcttcagg
     4201 ccagcgtcga aagtctcctg atgtcgctcg gagcgcggcc ggatctcggt ggcgccgacc
     4261 cgggaggcga tgctcgaagg catgtattcc ttgagcgcga cgttgcgttg cagggagtgg
     4321 tccgaggcga ggtagacgat gccgaagccg ccctcgccga tcgtcttcgt gatctcgaat
     4381 tcggcgaggc gtgttcccgc gggcagcgtc agggcgtgct gctccccgcc tgccggggcc
     4441 gcgacaggcg gggttgtggc gggcgatccg gttgtcggcg cgaagccgcc tgtcgcgtga
     4501 ggcgtcgtcg ccgccgcggt ccgctcgaat gcggcgcccg gcgtcgtcga cgtcgtgcgc
     4561 gcgccgggcg ggcggatgac tgtgcgatcg tcctcgtcgg aatccatgct cgtgtgcgac
     4621 gcaacgcgcc tgggctggcg cgccatcggc cgggtcggcg gatattagcg aactcgtaca
     4681 gaacggcctg cgctcttgcc agcgtggcgg cgcgcgaagg gcccgttcgt gcgttcggtc
     4741 gcgcgtcgcg gcaccgcgcg tggggcggct cgacgtcgac aatccgtcat cgccatctgg
     4801 aaggagcgcg cgcgtgaaga cctacgacat cgaagtccag cacctcaaat cggcgcgcca
     4861 cgacaagggc ctgatcgagc tcgaggtcga cgcgctcgtg ctgccgcgtc cggcacgcgg
     4921 cgaagaagag cgagcaagca gtcttcgcct gccggtggag cacgctcgat cgctgctgat
     4981 cctgctcagg cagcaacttg gcgagctcga caggctcaac ccgcgcagcc gtcgctccgg
     5041 gcgggcgtga gccttccacc gcgtcgcggc gcacgagccg ggatcgccgc ccgctcgtcc
     5101 catcttcttc gggcccggcg gccacttcga cgtatcgttg cgcgccgatt cgtctccccg
     5161 tcatgccctt gccatccgtg atccgctgca ctcccgtc
//
LOCUS       DASRWS010000525         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1082371, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000525 DASRWS010000000
VERSION     DASRWS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082371"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..581
                     /locus_tag="VFF72_09845"
     CDS             144..581
                     /locus_tag="VFF72_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93509.1"
                     /translation="MAASTAHFTLAHGSQAWKRATAVARPARRAESGFDVFMRKCVTV
                     ATVLDKALKRQVCVDGVNYMVALDPERIRLTRKGKRRPEVELRWRDLVSGEAAMAVAL
                     NASLVARPAAAKSVSGRARAPAAPAPRAAAPAAPKKRAARKPR"
     gene            586..897
                     /locus_tag="VFF72_09850"
     CDS             586..897
                     /locus_tag="VFF72_09850"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HZV93510.1"
                     /translation="MSTPVTIDVPHQLGRIEARRRIEAGFAKVVHSLPGGAGAYSERW
                     EGDRLVFSAAAMGQTVSGVIDVLDTMVKMEIVLPGVLGLLAGGLKRQLQKAGMLLLTK
                     K"
     gene            complement(1051..1182)
                     /locus_tag="VFF72_09855"
     CDS             complement(1051..1182)
                     /locus_tag="VFF72_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93511.1"
                     /translation="MALEKKKREGKKTIPASQVTKTRSGAKRRKSDGTQRPQTPATP"
     gene            1427..1822
                     /locus_tag="VFF72_09860"
     CDS             1427..1822
                     /locus_tag="VFF72_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZV93512.1"
                     /translation="MALDVLPLTYACHCLDCQTWSGSAFSQQAFVPEAALAVSGSVVI
                     YEFTTPSGNTSTQRMCGVCHARIYNTNSARPNVAVVRAGTLDRSDELRVVAHIWVKRK
                     QPWLTLAEGVPTWPEAAPAAELAGALTGH"
     gene            2089..2535
                     /locus_tag="VFF72_09865"
     CDS             2089..2535
                     /locus_tag="VFF72_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93513.1"
                     /translation="MACFNPKPCLAAALLVSSASALAGSVAPCDWVTLAQVKAAMGVD
                     MNAGTPIFDTGCSWHGRAAKVSVTLSSQLASKWWAAIQAPIAPYVKTPASGIGDEAVY
                     IKAGNLVWLSVKKGDRVLNVKVYGVSDEATQESTESALALEALQKF"
     gene            complement(2593..>3585)
                     /locus_tag="VFF72_09870"
     CDS             complement(2593..>3585)
                     /locus_tag="VFF72_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZV93514.1"
                     /translation="AKFSPQHKIELRVATIPTNNHLEDVVLRILASAQPIPIGMLGFA
                     EPIEKALLSVVERPYGMVLCVGPTGSGKTTTLHSALSHINVPERKIWTAEDPVEITQP
                     GLRQVQVNPKIYWTFAKALRAFMRADPDVIMLGEIRDEETAHMAIEASLTGHLVLSTL
                     HTNSAPETVTRLLDMGMDPFNFADSLLAVLAQRLVRRSCPHCRTSRAATHEEIEELLG
                     DYMHSFGAQAPLTRADVLAGWTRRHAEGGRLMMHKSIGCEKCDHTGLKGRAGIHEMMV
                     VSREVRGLIQNGARAEALQAAAMKEGMRTLRQDGIEKVLAGQTTIEEVRATSNG"
BASE COUNT          553 a         1150 c         1271 g          611 t
ORIGIN      
        1 gctcccgtca cactgctctg ccaccggccc atgacggggc tggccggcgc aaacccgaac
       61 gtgcgctatc gcgtgaagct caggagcacg gagagtctgc gcagccggat tcatgcgcgg
      121 cggtcgctcg aggcttgccg ctcatggctg cgtccacggc gcacttcacc ctggcccacg
      181 gcagccaggc gtggaagcgg gcgacggcgg ttgcgcgacc ggctcgtcgc gcagaatccg
      241 ggttcgatgt cttcatgcgg aaatgcgtca ccgtggctac tgtcctcgac aaggcactca
      301 agcggcaggt ctgcgtcgac ggcgtgaact acatggtcgc gctcgacccc gaacggatcc
      361 gcctgacccg caagggcaag cgtcggcccg aagtcgaact gcgctggcgc gatctcgtga
      421 gcggtgaagc cgcgatggcg gtggcgctga atgcgtcgct ggttgcgcgg ccggcggccg
      481 ccaagtcggt gtccggacgc gcacgggcgc cagcggcgcc cgcacctcgc gcagcggcgc
      541 ctgctgcgcc gaagaagcgg gctgcgcgca agccgcgata gacggatgtc gacgccagtc
      601 accatcgacg tgccgcatca gctcggccgc atcgaggcgc gtcgccgcat cgaggcgggc
      661 ttcgcgaagg tcgtgcattc cttgcccggc ggcgcgggcg cctacagcga gcgctgggaa
      721 ggcgacaggc tcgtcttcag cgccgccgcc atgggccaga cggtctcagg cgtcatcgac
      781 gtcctcgaca cgatggtgaa gatggagatc gtgctgcccg gagtgctggg cctcctggct
      841 ggcggcctga agcgtcagct gcagaaggcg gggatgctgc tgctgacgaa aaagtgacgc
      901 ggcaccggcc cgatgacagc cggcgacacg cggatcgtgc ttgccgccgc catgatcgtc
      961 acggagatgc agagggagcg gccgctgagg tcagacctct cggagccgtc gtcggcaaag
     1021 tcggtgccgg ccggtcgtcg cttccatcga tcagggagtg gctggcgtct gcggacgctg
     1081 ggttccatcg ctcttgcgcc gcttggcgcc gcttcgcgtc ttggtgacct gcgaggcggg
     1141 aatcgtcttc ttgccttcac gctttttctt ttcgagggcc atgtcgatct ccggtgatgt
     1201 gggacggagg atgcagccga agcgcgtgga gcggcagcgg aagaatccgc aagccgttgt
     1261 aggcgtccgg cccatggcgc acagcgatcc ccccgggacg aatggtccag cggggcggcg
     1321 gggcggacga tgctcgactt cggcatagtg gccgtctaca cattcgacgg agactggcat
     1381 gcaggaaatc gcgggagggt gccgatgtgg cgccctgcgc tacaggttgg cgctggatgt
     1441 gcttccgctc acctacgcct gccattgcct ggactgccag acctggagcg gcagcgcgtt
     1501 cagccagcag gcgttcgtgc ccgaggcggc actggccgtg agcggatcgg tcgtgatcta
     1561 cgagttcacg accccaagcg gcaacacctc gacgcaacgg atgtgcggcg tctgccatgc
     1621 ccgcatctac aacacgaaca gtgcccggcc gaacgtggcg gtcgtgcgtg ccggcacgct
     1681 cgaccgcagc gacgagctgc gggtcgtcgc gcatatctgg gtgaagcgca agcagccctg
     1741 gctgacgctg gcggagggcg tgccgacgtg gcccgaggcg gcgccggcag ccgagctggc
     1801 gggagcattg acagggcact gagcgccgcg gctcggtggt gcggatgcgc ggcggccagg
     1861 agcgccggtc atccgtcctg accgcgtgtc ggaactgctg cagacgtccc gcttgtgcaa
     1921 gcgacttgcc gcgggaagcg aggtcgcccg cttggaatgc acggcgccgc tcgctaaaat
     1981 ttcctgtgaa gaaggcaccg gttttgttgc tggcggccgc cgagcgagcg agtaccaagc
     2041 gccgagagga ttgctgcctc ccctgggctt tcttgtgaag gagcgagtat ggcctgcttc
     2101 aatccgaaac cctgcctggc tgccgcattg ctggtttctt cggcatcggc gctggctggc
     2161 tccgtggctc cctgcgactg ggtgacgctc gcgcaagtca aggcggcgat gggcgtcgac
     2221 atgaatgccg gtacgccgat attcgacacg ggttgcagtt ggcatggccg tgctgcgaaa
     2281 gtgagcgtaa cgctgtcttc ccagctcgcg tccaaatggt gggccgcgat ccaggcgccg
     2341 atcgcgccgt atgtcaagac acctgcttcc ggcattggcg acgaggccgt ctacatcaag
     2401 gcaggaaacc tggtttggct ttcggtcaag aagggcgacc gggttttgaa cgtcaaggtc
     2461 tatggagtga gcgacgaagc cacgcaggaa tcgaccgaaa gcgccctcgc gctggaggcg
     2521 ctgcagaagt tctaagcgca ggcgcgtgtg tgatccggcg gcggccgcaa gtgccgccca
     2581 cgcccccgcg cttcagccgt tgctcgtcgc ccgcacttcc tcgatcgtcg tctgtccggc
     2641 cagcaccttc tcgatgccgt cctggcgcag ggtgcgcatc ccttccttca tcgccgcggc
     2701 ctgcagcgcc tcggcccggg cgccgttctg gatcaggccg cgcacctcgc gcgagacgac
     2761 catcatctcg tggatgccgg cgcggccctt caggccggtg tgatcgcatt tctcgcagcc
     2821 gatgctcttg tgcatcatca gccggccgcc ttcggcatgg cgccgggtcc agccggcgag
     2881 gacgtcggcg cgcgtcaacg gcgcctgggc cccgaacgaa tgcatgtagt cgccgagcag
     2941 ctcctcgatc tcctcgtgcg tggccgcccg gctggtgcgg cagtgcgggc acgagcgtcg
     3001 cacgaggcgc tgcgccagca cggcgagcag cgaatcggcg aagttgaacg ggtccatgcc
     3061 catgtcgagc aggcgagtca ccgtttccgg tgcgctgttg gtgtgcagcg tcgagagcac
     3121 gaggtggccg gtgagcgagg cctcgatcgc catgtgcgcc gtttcctcgt cgcggatctc
     3181 gccgagcatg atgacatcgg gatcggcgcg catgaaggcg cgcagcgcct tggcgaaggt
     3241 ccagtagatc ttcggattga cctgcacctg gcgcaggccg ggctgggtga tctcgaccgg
     3301 atcctcggcc gtccagatct tgcgctcggg cacgttgatg tggctgagcg ccgaatgcag
     3361 cgtcgtcgtc ttgcccgagc cggtcgggcc gacgcacagc accatcccgt acgggcgctc
     3421 gacgacgctc aagagcgcct tctcgatcgg ctcggcgaag ccgagcatgc cgatcgggat
     3481 cggctgcgcc gaggcgagga tgcgcaggac gacgtcctcg aggtggttgt tggtcgggat
     3541 cgtcgcgacg cgcagctcga tcttgtgctg cggcgagaac ttggc
//
LOCUS       DASRWS010000526         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_503184, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000526 DASRWS010000000
VERSION     DASRWS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503184"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFF72_09875"
     CDS             <1..772
                     /locus_tag="VFF72_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93515.1"
                     /translation="YNRVILVLKGVSLQVREGSIVALLGGNGAGKTTTLRAVSNLLAG
                     ERGAVTKGSITLRGERIERLSTSDMVERGVIQVMEGRHCFAHLTIEENLMTGAWTRRD
                     GNARIAQTLDKVYTYFPRLKERRRSQAAYTSGGEQQMCAIGRALMASPKMVLLDEPSM
                     GLAPQIVGEVFEIVRDLNEREGVTFLLAEQNTNMALRYANYGYILESGRIVMDGAAAA
                     LRENEDVREFYLGAGAASGERKSYRDVKSYKRRKRWLS"
     gene            786..1058
                     /locus_tag="VFF72_09880"
     CDS             786..1058
                     /locus_tag="VFF72_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93516.1"
                     /translation="MARAEADALQKLLPLLWQLREIKGVQEIRPGVFHARREAFIHFH
                     DEGGALVAELKKAGGSGFDRYPLETAPQQRKLVDEARLRAKRLDDE"
     gene            1055..2347
                     /locus_tag="VFF72_09885"
     CDS             1055..2347
                     /locus_tag="VFF72_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV93517.1"
                     /translation="MSSHRASSDGPFFDVLETREPEARDAAQFASLSTLIAHAKARVP
                     AVELRLRDIDPAAINSRVALATLPVLRKHELLEAQAAHPEGDPFGGFAAIGWGRGRSH
                     ATRASRVFASPGPIHEPEGHRPDYWRMGRALFAAGIRAGDLVHNTFSYHFTPAGAMMD
                     SGARSIGCTVFPAGTGQTDLQVQTMKALSPDAYVGTPSFLRLILEKADDLGVALPSLR
                     TASLGGEAFPAALRSLLTSRGIAASQSYGTADLGLIAYETEAHEGLVIDEGVIVEIVR
                     PGTGDVLVDGEVGEVVVTALNPDYPLIRFGTGDLSAILPGRCPSGRTNRRIRGWLGRA
                     DQSVKVRGMFVHPPQVAEIARRHPGIRRARLVVAGEMADDRMSLQIEIEGAVPEGLAE
                     RVTETIRDVTKLRGTVEIKAPGTLANDGKVIDDQRRPA"
     gene            complement(2374..2817)
                     /gene="dtd"
                     /locus_tag="VFF72_09890"
     CDS             complement(2374..2817)
                     /gene="dtd"
                     /locus_tag="VFF72_09890"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZV93518.1"
                     /translation="MIALLQRVMAARVEVGPRVVGAIGHGLLVFVCAEPEDDDAIADR
                     LVDKVLRLRVFADAAGKMNLDLPTVEGGLLLVSQFTLAADTSRGTRPGFSRAAPPDLG
                     RRLYERALLTASRRHPVVACGEFGADMRVHLINDGPVTIPLAVRD"
     gene            complement(2814..3716)
                     /locus_tag="VFF72_09895"
     CDS             complement(2814..3716)
                     /locus_tag="VFF72_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZV93519.1"
                     /translation="MAAVQVRTLLRLSVGAAVAAITMKGLGWWLTGSVGLLSDAMESF
                     VNLASALFALAMVSIAERPADDDHPFGHSKAEYFASGFEGLLIAGAAIAIVWTAVQRF
                     MHLQSLQSVGVGLALVVASSLLNGALAWKMMAASRQHRSIALEADARHLYADVWTSVG
                     VVVGVGLVWLTGWLWLDPAIAILVAINIVREGTRVVWRSVDGLMDKALEPDVQARIDS
                     TLARFSQPAVRFDDMVTRRAGRRRFLDMHMHVPGSWTLGRAAALRGEVEEALMREVDG
                     LRASIQLLPSNREAAFNSVEESAP"
     gene            complement(3717..>4604)
                     /gene="tyrS"
                     /locus_tag="VFF72_09900"
     CDS             complement(3717..>4604)
                     /gene="tyrS"
                     /locus_tag="VFF72_09900"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZV93520.1"
                     /translation="AKTYYAQASLVLDGARTEIRYNSEWSDPLGARGMIQLASRYTVA
                     RMMERDDFAKRFKAGTPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRSLQ
                     AEYGQEPQCIMTMPLLEGLDGVEKMSKSKGNYIAISEPANDMFAKLMSISDALMWKYF
                     DLLSFRRADEIERLRLEVDSGRNPRDAKVLLAKEITARFHGAAAAEAGQADFNLRARG
                     GIPDEIESVTLVGAPLGIGAVLKQARLAPSTSEALRLVDGGGVRIDGAVVVDRGLKLP
                     AGTFVVQVGKRKFARVTLP"
BASE COUNT          770 a         1607 c         1578 g          649 t
ORIGIN      
        1 ctacaaccgg gtcatcctgg tgctcaaggg cgtgtcgctg caggtgcggg aaggcagcat
       61 cgtcgcgctg ctcggcggca acggcgccgg caagacgacg acgctgcgcg ccgtgagcaa
      121 cctgctcgcc ggcgagcgcg gcgcggtcac caagggctcg atcacgttgc gcggcgaacg
      181 gatcgagcgg ctgtcgacat ccgacatggt cgagcggggc gtcatccagg tgatggaggg
      241 gcgccactgc ttcgcccacc tcacgatcga ggaaaacctc atgaccggcg cctggacgcg
      301 ccgcgatggc aacgcccgga tcgcgcagac gctcgacaag gtctacacct actttccgcg
      361 cctgaaggag cggcgccggt cgcaggcggc ctacacctcg ggcggcgagc agcagatgtg
      421 cgccatcggc cgcgcgctga tggccagccc gaagatggtg ctcctcgacg agccgtcgat
      481 ggggctggcg ccgcagatcg tgggcgaggt cttcgagatc gtccgcgacc tcaacgagcg
      541 cgaaggcgtg accttcctgc tcgccgagca gaacacgaac atggccctgc gctacgccaa
      601 ctacggctac atcctcgaga gcggccgcat cgtcatggat ggcgccgccg ccgcgctgcg
      661 cgagaacgag gacgtgcgcg agttctatct cggcgccggc gcggcgtcgg gcgagcgcaa
      721 gagctaccgc gacgtcaaga gctacaagcg gcgcaagcgc tggctgtcct gagaggagac
      781 cggagatggc ccgagccgaa gccgacgccc tgcagaagct gctcccgctg ctgtggcagt
      841 tgcgcgagat caagggcgtg caggagatcc gccctggcgt cttccacgca cggcgcgagg
      901 cattcatcca tttccatgac gaaggcggcg ccctcgtcgc cgaactgaag aaggccggcg
      961 gcagcggctt cgaccgctat ccgctcgaga cggcaccgca gcagcgaaag ctcgtcgacg
     1021 aggcgaggct gcgcgccaag cggctcgacg acgagtgagc tcgcatcgcg cctcgagcga
     1081 tggcccgttc ttcgacgtcc tggaaacgcg agagccggag gcgcgcgacg ccgcgcaatt
     1141 cgcgtcgctg tcgacgctga tcgcgcacgc caaggcgcgc gtgcccgcgg tcgagctccg
     1201 gcttcgcgac atcgaccctg ccgcgatcaa ctcgcgcgtc gcgcttgcga cgttgccggt
     1261 cctgcgcaag cacgaactcc tcgaggcgca ggcagcgcat cccgagggcg atccgttcgg
     1321 cggcttcgcc gcgatcggct ggggtcgcgg ccgttcgcac gcgacccgcg cgagccgcgt
     1381 cttcgcgtcg ccgggtccca tccacgagcc ggaaggtcac cgccccgact actggcgaat
     1441 gggccgtgcc ctcttcgccg cgggcattcg cgcgggcgac ctggtgcaca acaccttcag
     1501 ctaccacttc acgccggccg gcgcgatgat ggacagtggc gcgcggtcga tcggctgcac
     1561 ggtgtttccg gcgggaaccg gccagaccga cctgcaggtg cagacgatga aggcgctgtc
     1621 gcccgatgcc tacgtcggca cgcccagctt tcttcgcctg attctcgaga aggccgacga
     1681 cctcggcgtg gcgctcccct cgctgcgcac ggcgtcgctc ggaggcgagg ccttcccggc
     1741 ggcgctgcgc agcctgctca ccagccgcgg catcgccgcg agccagagct acggcaccgc
     1801 cgatctcgga ctcatcgcct acgagaccga ggcgcacgaa gggctggtga tcgacgaagg
     1861 cgtgatcgtc gagatcgtcc gccccggcac cggcgacgtc cttgtcgacg gcgaggtggg
     1921 cgaagtcgtc gtcactgccc tgaaccccga ctacccgctg atccgtttcg gtaccggcga
     1981 cctctcggcg atcctgccgg ggcggtgtcc cagcgggcgc accaaccggc gcatccgtgg
     2041 ctggctcggc cgcgccgatc agtcggtcaa ggtccgcggc atgttcgtgc atccgccgca
     2101 ggtggcggag atcgcccgac gccaccccgg gatccgtcgc gcgaggctcg ttgtcgccgg
     2161 cgagatggcc gacgaccgga tgagcctgca gatcgagatc gagggcgcgg tgccggaagg
     2221 cctcgccgag cgcgtcaccg agacgatccg cgatgtcacc aagctgcgcg gcaccgtcga
     2281 gatcaaggcg cccggaacgc tggccaacga cggcaaggtg atcgacgacc agcgtcgacc
     2341 cgcctgaggc gaggcggtgg cggcgccgcg aactcagtct cgaaccgcca ggggaatcgt
     2401 caccggtccg tcgttgatca ggtgaacgcg catgtcggca ccgaattcgc cgcaagccac
     2461 gaccggatgg cgccgcgatg ccgtcagcaa cgcccgctcg tagaggcgac ggccgagatc
     2521 gggcggcgcc gcgcgagaaa atcccggccg ggtgccgcgc gacgtgtccg ccgccagagt
     2581 gaactggctg accaggagca ggccgccttc gaccgtcggc agatcgaggt tcatcttgcc
     2641 ggcggcgtcg gcgaacacgc gcaacctcag caccttgtcg acgaggcggt cggcgatcgc
     2701 gtcgtcgtcc tcgggctcgg cgcagacgaa aacgagaagg ccgtgcccga tcgcaccgac
     2761 aacccgcgga ccgacctcga cccgcgccgc catcacgcgc tgcagcagcg cgatcagggc
     2821 gcggactcct cgacgctgtt gaaggccgcc tcgcggttcg acggcaggag ctggatcgac
     2881 gcacgcaggc catcgacctc gcgcatcaat gcttcctcga cctcgccgcg caacgccgct
     2941 gcgcgaccga gcgtccacga acccggcaca tgcatgtgca tgtcgagaaa acgcctgcgg
     3001 ccggcgcgcc gggtcaccat gtcgtcgaag cggacggcgg gctggctgaa gcgggcgagg
     3061 gtcgaatcga tccgcgcctg cacgtcgggc tccagcgcct tgtccatcag cccgtcgacc
     3121 gaacgccaga cgacgcgcgt gccctcgcgg acgatgttga tcgcgaccag gatggcgatc
     3181 gccgggtcga gccacagcca gccggtgagc cagacgaggc cgacccccac gacgacgccg
     3241 accgaggtcc agacgtcggc gtagagatgg cgggcgtcgg cttcgagcgc gatcgatcgg
     3301 tgctggcgcg aggccgccat catcttccag gcgagcgcac cgttgagcag cgagctcgcg
     3361 acgaccagcg ccaggcccac gcccaccgac tgcagcgact gcaaatgcat gaagcgctgc
     3421 acggccgtcc agacgatggc gatggccgcc ccggcgatca gcaggccctc gaagccgctg
     3481 gcgaagtatt cggccttgct gtgaccgaag ggatgatcgt cgtcggccgg gcgctcggcg
     3541 atgctcacca tcgccagtgc gaagagcgcc gacgcgaggt tgacgaagga ctccatcgca
     3601 tcggacagca gcccgaccga gccggtcagc caccagccca ggcccttcat cgtgatcgcc
     3661 gcaaccgccg cgccgaccga caggcgcagc agcgtgcgga cctgcaccgc cgccatctag
     3721 ggcaaggtga cgcgcgcgaa cttgcgcttg ccgacctgga ccacgaaggt gccggcggga
     3781 agcttcaggc cgcgatcgac gacgacggcg ccgtcgatgc ggacgccacc gccgtcgacc
     3841 aggcgaagcg cctcgctggt ggagggcgcc agccgtgcct gtttcagcac cgcaccgatg
     3901 cccagtggcg caccgacgag cgtcaccgac tcgatctcgt cgggaatgcc gccccgggcg
     3961 cgcaggttga agtcggcctg gccggcttcg gcggcggcgg cgccatggaa gcgggccgtg
     4021 atctccttgg ccagcagcac cttggcatca cgcgggttgc ggccggaatc gacctccaag
     4081 cgcagacgct cgatctcgtc ggcgcgacga aagctgagca ggtcgaagta cttccacatc
     4141 agcgcgtcgc tgatcgacat cagcttggcg aacatgtcgt tggccggctc ggagatggcg
     4201 atgtagttgc ccttggactt cgacatcttc tcgacgccgt cgaggccttc gagaagcggc
     4261 atcgtcatga tgcattgcgg ctcctgcccg tactcggcct gcagcgatcg gcccaccagc
     4321 aggttgaact tctggtcggt accgccgagc tcgaggtcgg atttcagcgc caccgagtcg
     4381 tagccctgca tcagcgggta gaggaactcg tgaacgctga tcggcgtgcc cgccttgaac
     4441 ctcttggcga agtcgtcgcg ctccatcatc cgcgccaccg tgtagcgcga ggcgagctgg
     4501 atcatgccgc gcgcgccgag cgggtcgctc cactcggagt tgtagcggat ctcggtcctg
     4561 gcgccgtcga ggaccaggct tgcctgcgcg tagtaagtct tcgc
//
LOCUS       DASRWS010000527         7857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_798874, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000527 DASRWS010000000
VERSION     DASRWS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798874"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFF72_09905"
     CDS             <1..608
                     /locus_tag="VFF72_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HZV93521.1"
                     /translation="PLRSGQFKVAAFYSINNCQPGLRGVSLGNFLIKRVAEELKVELP
                     QLKIFCTLSPIPGFSAWLESTTDFSQLGLVPRAATLRLPEARALVRDALHGDADGLAS
                     ATSLRAMPPKAQAALLRLAATYLLHMSTRTGGDPVARFHLANGARLERLDWLADLSRS
                     GLKQSCGLMVNYLYDLQRIETNHEKFIHGEVPHTRAVAALI"
     gene            1193..1633
                     /locus_tag="VFF72_09910"
     CDS             1193..1633
                     /locus_tag="VFF72_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93522.1"
                     /translation="MMKALLALAGTACLAAMPVAAEANCFFVYSAQNQLVYRSTISPV
                     DLSRPISDGMRGRFAGDHLTMIPDETNCPDLLVGGESQVFASFGFPSAGRSVSAIDAS
                     PLFRGLDSPGGGASTTYDGAAVTPAGGSTGRAPRRGRSGAAAGR"
     gene            1755..2615
                     /locus_tag="VFF72_09915"
     CDS             1755..2615
                     /locus_tag="VFF72_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZV93523.1"
                     /translation="MPSAAVGDTGASIDRAATLSEAQAMLGGEAGYDAIVLDAASVVV
                     SAPEVEAIGARAALVVLVTEPDAEHALGWLRRGADDVLSPEELGSAAGWRRVRFAIER
                     RRRLDGTQPAYATDPATGLPHRQQLVEHLSQLLALREREPAPMAVLALRLELPNAVDE
                     SGQAGLLLRKIAVRLRAAVRSSDVVASTGDDCFVVLLGAVMAPAEAARVGDKLAAVLV
                     APFKVGGGELAVAAAVGVAHYPQDGNQADRLLRRALALAAVAPPLTFGETADIRDGAA
                     SDRLAANDEH"
     gene            2870..5509
                     /gene="alaS"
                     /locus_tag="VFF72_09920"
     CDS             2870..5509
                     /gene="alaS"
                     /locus_tag="VFF72_09920"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZV93524.1"
                     /translation="MKTSEIRSTFLKFFESKGHTVVASSPVVPGDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYVRAATAQKCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDALKFAWELLTEVYRLPKDKLWATVYAEDDEAYAIWKDDIGLPVERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCSEIFYDHGPEIAGGPPGSPDEDGDRYIEIWNLVFMQFERS
                     ADGAMTKLPKPSVDTGMGLERLTAVLQHVHSNYEIDLFVTLLAAAKRVVELAGATDVD
                     ADSPSLKVIADHIRACTFTVVDGVIPGNEGRGYVLRRIARRAIRHGYKLGARRPFFHK
                     LVADLGDAMGDAYPELRRDAGRATDVLRQEEERFFQTIANGMDILDAAIASLPSRGER
                     ILAGDVAFKLHDTYGFPLDLTADVGRERGVAVDTAAFDVAMARQRDQARAAGRFKVAQ
                     GLEYSGAATTFHGYEQLTRDSARVVAVYVDGSPVASARAGDDAVVVLDHTPFYAESGG
                     QVGDRGELRNATSRMRVEDTVKIQATVFGHEGRVLEGEIRVGDSVDAKVDVERRARTV
                     RNHSATHLMHKALRAVLGEHVQQKGSLVDADKTRFDFAHNAPLTDEQIGRVERIVNAE
                     ILANHATSARVMPIEDAQGLGAMMLFGEKYGDEVRVLDIGTSRELCGGTHVARTGDIG
                     LFKVVSEAGVASGIRRIEAVTGEGALAYLQGLESTVRRVAGTLHSAPAEVPQRLAQVL
                     DQTRALEKEMAALKGRLASSQGGDLAGRAIDVKGLKVLAAQLDGADAKTLRETLDKLK
                     DKLKTAVVVLATVDGAKVQIAAGVTADATSRVRAGDLVNFVAHQVGGKGGGKADMAMA
                     GGTDASRLGEALASVAGWVGERA"
     gene            5544..5894
                     /locus_tag="VFF72_09925"
     CDS             5544..5894
                     /locus_tag="VFF72_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZV93525.1"
                     /translation="MDELFLEGLEVPCRVGCTDPERATPQSLRVGVRLFCPTLRDAAI
                     ADDLEKTVDYRLAGELIDAVQGREFLLIERVAEELAAVVLRNPLVDHVIVSVRKRPPV
                     QGLEWAGVQITRGR"
     gene            5891..6544
                     /locus_tag="VFF72_09930"
     CDS             5891..6544
                     /locus_tag="VFF72_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HZV93526.1"
                     /translation="MKVFSNTAISLDGRINTKEGGFRFFGSPRDHARMSRLRAEADAI
                     LVGGATFRNWPHPSLPDDVDRTQAHRPWWNVVVTRTLDVPLDAGFLAEPAVRPLFLTR
                     ADSLRAGFAAEAEGWSGPGSSLPVAWILAQLERRGVERLLVEAGGDLLFQFLAADAID
                     EMNLTLCPLVAGGPAPGLVGGPGFDFAAARRFRLEAAVPEGDEVFLRYAAVHRQEAR"
     gene            6541..7089
                     /locus_tag="VFF72_09935"
     CDS             6541..7089
                     /locus_tag="VFF72_09935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV93527.1"
                     /translation="MTAITIRRAVPGDAAAFARIMGDPLVYPALLQMPHPSEEGWRSR
                     LAEFGAPGKSDFMIVAERDGEVVGNAGLHPAGIQIRRRHVGAIGICVAADAQGKGVGK
                     ALMQALCDYADGWMNLLRIELIVFADNAPAIALYRKFGFRIEGRHLGYAMRDGRYADA
                     LSMARLHPRPPSIEPGAVDDVA"
     gene            7115..>7857
                     /locus_tag="VFF72_09940"
     CDS             7115..>7857
                     /locus_tag="VFF72_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZV93528.1"
                     /translation="MSQSRTAARRDSPFLLRAARIGLGLLVALLLMPASALAQERVSF
                     PSLDGEASAPIVLTAFWFPASVSPAPAVVLLHGCGGMFDRHRQLDRRMRDYSALFNAA
                     GYHVLVVDSLTPRYETELCTQRIGKRRVTQANRRLDALGAVAYLAQRSDVDASRIGLV
                     GWSNGASTVLAATNLRHPDVAAALVRPAFGIAFYPGCEADLRRGYEPAGPVLLLAGGA
                     DDWTPVAPCRALVRAAADPKPTLVVYPGA"
BASE COUNT         1148 a         2729 c         2723 g         1257 t
ORIGIN      
        1 caccgctgcg atccgggcag ttcaaggtgg cggcgttcta ctccatcaac aactgccagc
       61 ccggcctgcg aggcgtctcg ctcggcaact tcctgatcaa gcgcgtcgcc gaggagttga
      121 aggtcgagct gccgcaactg aagatcttct gcaccctctc gccgatcccc ggcttttccg
      181 cgtggctcga gtccaccacc gatttcagtc agctcgggct cgtgccgcgg gctgcaaccc
      241 tgcgcttgcc cgaggcgcgc gcgctcgtcc gcgacgcctt gcacggcgac gccgacgggc
      301 ttgcgagcgc cacctcgttg cgcgcgatgc cgccgaaggc gcaggcggcg ctgctgcgcc
      361 tggcggcgac ctacctgctc cacatgtcga cacgcacggg aggcgacccg gtggcgcggt
      421 tccacctcgc caacggtgct cgcctcgagc ggctcgactg gctcgccgac ctgtcgcgca
      481 gtggcctcaa acagtcgtgc gggctgatgg tcaactacct ctacgacctg cagcggatcg
      541 agaccaacca cgagaaattc attcacggcg aagtgccgca tacgcgcgcc gtggccgcgc
      601 tgatatgagc ggcacctgga tgagcggcac gtggcgtcgc ttcggatcga cgagcttgcg
      661 gtcgaagtcc ctggttgacg aaggattcgc gttcgccacg atctgatttc gaagtcgcgg
      721 attcccgcca cgagcgatcg ccgaggcacc cgtaccgcgt ctctcgttcg cgaaccgcat
      781 ccatcgaccc ggccatcgtg tcgatgttca cgaagcctcg caactcgtcg cacggcggcg
      841 cgcctagagt gcgttgttgc ttcgcaggaa gggatcgatg cgttggcgta cgctcgcggc
      901 ggcagtttcc tgccttgccg cctttctggc cacgccggca acggcggcgg cgcaggtctt
      961 caggtgtccg gtcggcggtg caatcgtgtt ccagcaatcg ccttgtgccg ggctcgcagc
     1021 gagcggtggc cggttgctga tcggggccga cggccagaag gtcgccgagc ggcgcgcgga
     1081 gccggaaccg ctgccgccgc cgcgcgttct gggccggaca cctttgccta cacccatggg
     1141 tgccaggcac cgcaagtcgc cttaaagtcg agtcttcgga ggatcgagca tcatgatgaa
     1201 agcacttctc gcgctcgccg gcaccgcgtg cctggctgcc atgcccgtcg ccgccgaggc
     1261 gaattgcttc ttcgtctact cggcgcagaa ccagctcgtc tatcgctcga cgatctcgcc
     1321 tgtcgatctg tcgcgtccca tttcggacgg gatgcgcggg cgcttcgcgg gcgatcacct
     1381 gacgatgatt cccgacgaga cgaactgtcc tgaccttctg gtcggcggcg agagccaggt
     1441 gttcgcgtcg ttcggtttcc cgtctgcggg ccggtccgtt tcggcgatcg atgcatcgcc
     1501 gctctttcgc ggcctcgatt cgcccggcgg cggcgcgtcg accacgtacg acggggccgc
     1561 ggtgacgccc gccggaggtt cgaccgggcg tgcgccacgc cgtgggcgct ccggcgccgc
     1621 cgcgggccgc tgaccggaca ccaccgccgc gcggcgctcc gacggcgctg cccgcccatc
     1681 gccatgacag ctgcctcatc gccatgacag cggcttcgcc gaggcttctt cttgtcggcg
     1741 cggcacctga ccccgtgcct tcggcggcag tgggcgacac cggcgcgagc atcgatcgag
     1801 cggcgacgct ttccgaggcg caagccatgc tgggtggcga ggccggctac gacgccatcg
     1861 tgctcgatgc ggccagcgtc gtcgtctcgg cgccggaagt cgaagcgatc ggcgcccgtg
     1921 ccgcacttgt ggtcctggtc accgagcccg atgccgagca cgccctcggc tggctgcgcc
     1981 gcggcgccga cgacgtcctc tcgcccgagg agctcggctc ggcggcgggc tggcgacgtg
     2041 tccgcttcgc gatcgagcgg cggcgccgtc tcgacggaac gcagccggcc tatgccaccg
     2101 accccgcgac cggcttgccg caccgccagc aacttgtcga gcatctcagc cagttgctcg
     2161 cgttgcggga gcgcgagccg gcgccgatgg cggtgctggc cttgcgcctc gagctgccga
     2221 acgcggtcga cgagtccgga caggccggcc tgctgctgcg aaagatcgcc gttcgactgc
     2281 gcgccgcggt tcgctcgagc gatgtcgtcg cctcgacagg ggacgactgc tttgtcgtcc
     2341 tgctcggtgc ggtgatggcc cccgccgagg cggcgcgggt cggcgacaag ctcgccgccg
     2401 tcctggtggc gccgttcaag gtcggtgggg gcgagcttgc ggtcgccgcg gctgtcggcg
     2461 tcgcccacta tccgcaagac ggcaaccagg ccgaccgcct ccttcggcgc gccctcgcgc
     2521 tggccgcggt ggcaccgccg ctgaccttcg gagaaactgc agacatccgc gacggcgcgg
     2581 cttcggatcg cctggccgcg aacgacgagc actgatcggg cgacgttgct gcccgatcgg
     2641 ggccgcggtg cgggcaggga caacgcctgc ggaccgctgc gtgcgaggcg cgctgatatc
     2701 ctcgcgagtg caagcgacga cagtctgcgc gggctgtcgc ccgtggccag acgccgggcg
     2761 aagccggatg aagcgtctgc atgcaggcga gcgggatctg cggccggcga agcctggatg
     2821 ccgagcggtt cgccccctca acctattccg agcgccacga aacgcggcca tgaaaacctc
     2881 cgagatccgt tccacgttcc tgaagttctt cgagtcgaag gggcacacgg tcgtcgcctc
     2941 gagcccggtc gtgccgggcg acgatccgac gctgctcttc accaatgccg ggatgaacca
     3001 gttcaaggac gtcttcctcg gtttcgacaa gcgcccctac gtgcgcgccg cgacggcgca
     3061 gaaatgcatc cgcgccggcg gcaagcacaa cgacctcgag aacgtcggct acaccgcgcg
     3121 gcaccacacg ttcttcgaga tgctcggcaa tttcagcttc ggcgactact tcaagaaaga
     3181 tgcgctgaag ttcgcgtggg aactgctcac cgaggtctac cggcttccga aggacaagct
     3241 ctgggcgacc gtctacgccg aggacgacga ggcctacgcc atctggaagg acgacatcgg
     3301 cctgccggtg gagcggatcg tgcgcatcgg cgacaacaag ggcgcgcgct acgcgtccga
     3361 caacttctgg atgatgggcg acacgggccc gtgcggcccg tgcagcgaga tcttctacga
     3421 ccacggtccc gagatcgccg gcggcccgcc aggctcgccc gacgaggacg gcgaccgcta
     3481 catcgagatc tggaatctcg tgttcatgca gttcgagcgc agtgccgacg gcgcgatgac
     3541 gaagctgccg aaaccctcgg tcgatacggg catgggcctc gagcggctga cggcggtgct
     3601 gcagcacgtg cattcgaact acgagatcga cctgttcgtg acgctgctcg cggcggcgaa
     3661 gcgggtggtc gaattggcag gggcgaccga tgtcgatgcc gattcgccct cgctcaaggt
     3721 cattgccgat cacatccgcg cctgcacctt cacggtggtc gacggcgtca tcccgggcaa
     3781 cgaaggtcgc ggctacgtcc tcaggcggat cgcgcgccgg gcgatccggc acggctacaa
     3841 gctcggcgcg cgccggccgt tcttccacaa gctggtcgcc gacctcggcg acgcgatggg
     3901 cgatgcctat cccgagctgc gccgcgatgc cggacgggcc accgacgtgc tgcgccagga
     3961 agaggagcgc ttcttccaga ccatcgccaa tggcatggac atcctggacg ccgcgatcgc
     4021 ctcgctgccg tcccggggcg agcgcattct tgccggcgac gtcgcgttca agctgcacga
     4081 cacctacggc tttcccctcg acctgacggc cgacgtcggg cgcgagcgag gcgtggcggt
     4141 cgacacggcc gcgttcgacg tggcgatggc gcgccagcgc gatcaggccc gcgcggcggg
     4201 tcgtttcaag gtggcgcagg gcctcgagta cagcggcgcg gcgacgacgt ttcacggcta
     4261 cgagcaactc acgcgcgaca gcgcccgggt cgtcgcggtc tacgtcgacg gcagcccggt
     4321 cgcgagcgcg cgcgccggcg acgacgccgt cgtcgttctc gaccacacgc cgttctatgc
     4381 cgagtcgggc ggacaggtcg gcgatcgtgg cgagctgcgc aacgccacgt cgcgcatgcg
     4441 cgtcgaggac acggtgaaga tccaggccac ggtgttcggc cacgaggggc gcgtcctcga
     4501 aggcgagatc cgcgtgggcg acagcgtcga cgcgaaggtc gacgtcgagc ggcgggcccg
     4561 aaccgtgcgc aaccacagcg cgacgcacct catgcacaag gcgttgcgcg cggtgctggg
     4621 cgaacacgtg cagcagaagg gctcgcttgt cgatgccgac aagacgcgct tcgatttcgc
     4681 ccacaacgcg ccgctgaccg acgagcagat cggtcgcgtc gagcggatcg tcaacgccga
     4741 gatactcgcc aaccacgcca ccagcgcgcg cgtcatgccg atcgaggacg cacagggcct
     4801 gggggcgatg atgctgttcg gcgagaaata cggtgatgag gtgcgcgtcc tcgatatcgg
     4861 cacgagccgg gagctttgcg gcggcacgca cgtggcgcgc accggcgaca tcgggctttt
     4921 caaggtcgtg tccgaagccg gagtcgcctc cggcatccgg cgcatcgaag cggtcaccgg
     4981 cgagggtgcg ctcgcgtacc tgcaggggct cgaatcgacc gtgcggcgcg tcgccggcac
     5041 cttgcattcg gcgccggccg aggtgccgca acgtctggcc caggtgctcg accagacgcg
     5101 cgctctcgag aaggaaatgg cggcgttgaa gggccggctg gcttcctccc agggcggcga
     5161 cctggctggc agggcgatcg acgtcaaggg gctgaaggtg ctggccgcgc aactcgacgg
     5221 cgccgacgcg aagaccttgc gcgagacgct cgacaagctc aaggacaagc tgaagaccgc
     5281 cgtcgtcgtc ctcgccaccg tcgatggcgc gaaggtgcag atcgccgccg gcgtcaccgc
     5341 cgatgcgacc tcgagggtga gggccggcga cctggtcaat ttcgtcgctc atcaggtcgg
     5401 cggcaagggc ggcggcaagg ccgacatggc gatggctggc ggcaccgacg catcgcggct
     5461 cggcgaagcg ctcgcctcgg tggcgggctg ggtcggggag cgcgcatgaa gcgggcgata
     5521 agcggccacg gcggagagcg ctgatggacg agctcttcct ggaaggcctc gaggtcccgt
     5581 gccgggtcgg ttgcaccgat cccgagcggg cgacgccgca atccctgcgc gtcggcgtgc
     5641 gcctcttctg tccgacgctg cgcgacgcgg cgatcgccga cgacctcgag aagacggtcg
     5701 actaccggct cgcgggcgag ctcatcgacg ccgtgcaggg tcgcgaattc ctgctcatcg
     5761 agcgggtcgc cgaggagctc gctgccgtgg tcctcaggaa tccgctggtc gaccacgtca
     5821 tcgtctcggt gcgcaagcgg ccgccggtgc aggggctcga gtgggccggg gtgcagatca
     5881 cgcggggccg atgaaggtct tcagcaacac cgcgatctcc ctcgacgggc gcatcaacac
     5941 gaaagagggc ggctttcgtt ttttcggctc gccacgcgac cacgcccgca tgagccggct
     6001 gcgtgccgaa gccgacgcca tccttgtcgg cggcgcgacg ttccgcaact ggccgcatcc
     6061 gtcgttgccc gatgacgtgg atcgaacgca agcgcatcgg ccctggtgga acgtcgtcgt
     6121 gacacgtacg ctcgacgtgc cgctcgacgc cggatttctc gccgagccgg cggtgcggcc
     6181 gctcttcctg acacgtgccg attcgcttcg tgccgggttc gcggccgagg cggagggctg
     6241 gagcggaccc ggcagcagcc tgccggtggc gtggatcctt gcgcaactcg aacggcgcgg
     6301 agtcgaacgc ctcctcgtcg aggccggggg cgacctgctg ttccagtttc tcgccgccga
     6361 cgcgattgac gagatgaacc tgacgctttg ccccctggtc gccggcggtc ccgcacccgg
     6421 tcttgtcggc ggcccggggt tcgacttcgc tgccgcacgg cgcttccggc tcgaggccgc
     6481 ggttcccgag ggcgacgagg tctttcttcg ctacgcggcg gtgcatcgac aggaggcgcg
     6541 atgacggcga tcaccatccg tcgggccgtc ccgggagatg ccgcggcttt cgcgcggatc
     6601 atgggcgatc cgctcgtcta tccggcgttg ctgcagatgc cgcatcccag cgaggaagga
     6661 tggcgcagcc gcctcgccga gttcggcgca ccgggcaagt ccgacttcat gatcgtcgcc
     6721 gaacgcgacg gcgaggtcgt cggcaacgcc ggcctgcatc cggccggaat ccagatccgc
     6781 cgccgccatg tgggtgcgat cggcatctgc gtcgcggccg acgcgcaggg caagggcgtc
     6841 ggcaaggcgc tgatgcaggc cttgtgcgac tacgccgacg gctggatgaa cctgttgcgg
     6901 atcgagctca tcgtctttgc cgacaacgcg ccggcgatcg ccctctatcg caagttcggc
     6961 tttcgcatcg agggccgtca tctcggctat gcgatgcgcg acgggcgcta cgccgacgcc
     7021 ttgtcgatgg cccggctgca tccccggccg ccatcgatcg agcccggagc ggtcgacgat
     7081 gtggcctgat cgcccgatga tgccgccgcc gtcgatgtcg caatcccgaa ctgccgcgcg
     7141 tcgcgactcg cccttccttc tgcgcgccgc ccggatcggc ctcggccttc tggtggcgct
     7201 gcttctcatg ccggcgtccg cgctggcgca ggagcgggtg tcgttcccga gcctggacgg
     7261 cgaggccagc gcgccgatcg tcctgacggc gttctggttt cccgcatcgg tgtcgccggc
     7321 gcccgccgtc gtgcttcttc acggctgcgg cggcatgttc gatcgtcatc gccagctcga
     7381 ccggcgcatg cgcgactact cggcgctctt caatgccgcc ggctaccacg tgctcgtcgt
     7441 cgactcgctg acgccgcgct acgagaccga gctgtgcacg cagcggatcg gcaagcgccg
     7501 cgtcacgcag gccaaccgcc gtctcgacgc gctgggcgct gtcgcctacc tcgcgcaacg
     7561 aagcgacgtc gacgcgagcc ggatcgggct cgtcggctgg tcgaacggcg ccagcacggt
     7621 gcttgccgcg accaacctgc gccaccccga cgtcgcggcg gcgctcgttc gtccggcatt
     7681 cggcatcgcc ttctatccgg gctgcgaggc tgacctgcga cggggctatg agccggcggg
     7741 tccggtgctg cttctcgccg gtggggccga cgactggacg cccgttgcgc cctgccgcgc
     7801 gctggtccgc gcggcggccg accccaagcc gacgctggtc gtctacccgg gcgcgta
//
LOCUS       DASRWS010000528         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1917297, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000528 DASRWS010000000
VERSION     DASRWS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917297"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFF72_09945"
     CDS             <1..266
                     /locus_tag="VFF72_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZV93529.1"
                     /translation="RIARRWLHRDNDKIPHAVKGQVAEAVALSPYLEKLVAMREELRQ
                     LWTRTNVSAEQLVADLQAWLHKAEESGIAVLQEFALRLRAAHA"
     gene            complement(388..555)
                     /gene="rpmG"
                     /locus_tag="VFF72_09950"
     CDS             complement(388..555)
                     /gene="rpmG"
                     /locus_tag="VFF72_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZV93530.1"
                     /translation="MAKGGREKIKLESSAGTGHFYTTDKNKKTTPEKIEMIKFDPKAR
                     KHVTYKETKLK"
     gene            complement(566..799)
                     /gene="rpmB"
                     /locus_tag="VFF72_09955"
     CDS             complement(566..799)
                     /gene="rpmB"
                     /locus_tag="VFF72_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZV93531.1"
                     /translation="MARVCQVTGKRPMVGNNVSHANNKTKRRFLPNLHYRRFWVEAEN
                     RWVRLRITNAALRLIDKVGIEQVVADLRARGEI"
     gene            complement(891..1847)
                     /gene="trxB"
                     /locus_tag="VFF72_09960"
     CDS             complement(891..1847)
                     /gene="trxB"
                     /locus_tag="VFF72_09960"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZV93532.1"
                     /translation="MTNPPQHARVLILGSGPAGYTAAIYAARANLKPMLVTGIAQGGQ
                     LMTTTDVDNWPADVDGVMGPDLMQRFLKHAERFDTRVVFDHVDEVDLRARPFTLRGDS
                     GHYSADALVIATGASAKYLGLASEQEFMGRGVSGCATCDGFFYRGQEVCVVGGGNTAV
                     EEALYLSNIASKVHVIHRRDKFRAEAILVDRMMAKVAEGKMQLHLWSTLDEVLGDASG
                     VTGVRLRGSRDGSTSTVAVTGCFIAIGHQPNTDIFKGQLEMRDGYILTRSGLDGFATM
                     TSVDGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRFLEQTS"
     gene            2019..>2407
                     /locus_tag="VFF72_09965"
     CDS             2019..>2407
                     /locus_tag="VFF72_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZV93533.1"
                     /translation="MAMLSNLDLIRRVPLFSLLTNDQAQGIADSVVKRRFRRGEIVVE
                     HGKKTNALFILLTGRARVLTADSRGREVILAVLQSGDYVGEMSLIDNEPHSATVRAEV
                     QTDMLILGRAEFARCLPENSSLSYAIMR"
BASE COUNT          385 a          783 c          800 g          439 t
ORIGIN      
        1 tgcggatcgc ccgccgctgg ctgcatcgcg acaacgacaa gatcccgcac gccgtgaagg
       61 ggcaggtcgc cgaggcggtg gcgctcagcc cgtatctcga gaagctcgtg gccatgcgcg
      121 aagagctgcg ccagctctgg acccgcacca acgtgtccgc cgagcagctg gttgccgatc
      181 tgcaggcgtg gctgcacaag gccgaggaaa gcggcattgc cgttctccag gagttcgcct
      241 tgcggctgcg ggccgcgcac gcgtagccca gggcttcgcc ggctgcggcc ggcaagacgg
      301 gtcgcctccg cctggcggtc gacccaaaat gaaaaagggt cgcgacgcga cccttttcct
      361 cgacacgacc gcaatcgcga ccgtggctta cttgagcttc gtttccttgt aggtcacgtg
      421 cttgcgagcc ttcggatcga atttgatcat ctcgatcttt tccggcgtcg tcttcttgtt
      481 cttgtcggtc gtgtagaagt gaccggttcc ggcgctcgat tcgagcttga tcttctcgcg
      541 tccacccttg gccatgacgg tttcctcaga tttcgccgcg ggcgcgcaga tcggcgacga
      601 cctgctcgat gcccaccttg tcgatcagcc gcagcgcggc attcgtgatg cgcaaccgca
      661 cccaccggtt ttccgcctcg acccagaacc ggcgatagtg cagattggga aggaagcggc
      721 gcttcgtttt gttgttggca tgggaaacgt tgttccccac catcgggcgc ttgcccgtga
      781 cctgacagac gcgtgccatg acgcttctcc gtgacttgct gatggaatgc gcacccgagg
      841 gccgagtggc aaaccgcgca ttatagcggt agccgccgag tggttgcgcc ctacgacgtc
      901 tgctccaaga aacgctgcgc gtcgagggcc gccatgcagc ccgtgccggc gctcgtgatc
      961 gcctgacggt agacgtggtc ctggacgtcg ccggcggcga agacgccgtc gacgctcgtc
     1021 atcgtcgcga agccatcgag gccgcttctc gtcaggatgt agccgtcgcg catctccagc
     1081 tggcccttga agatgtcggt gttgggttgg tggccgatgg cgatgaagca gccggtgacg
     1141 gcgaccgtcg acgtcgagcc gtcgcggctg ccgcgcagtc gaacgccggt gacgccgctg
     1201 gcgtcgccga gcacctcgtc gagcgtcgac cagaggtgca gctgcatctt tccttcggcc
     1261 accttggcca tcatcctgtc gaccaggatc gcctccgcgc gaaacttgtc gcgccgatgg
     1321 atgacgtgca ccttcgaggc gatgttggaa aggtagaggg cttcctccac cgcggtgttg
     1381 ccgccgccga cgacgcacac ctcctggccg cgatagaaga agccgtcgca cgtcgcacaa
     1441 ccgctcacgc ctcggcccat gaactcctgc tccgaggcca ggcccaggta tttggcggag
     1501 gcgccggtgg cgatgacgag ggcgtcggcg ctgtagtgtc cggagtcgcc gcgcagcgtg
     1561 aatggccggg cccgaaggtc gacttcgtcg acatggtcga agacgacccg ggtgtcgaag
     1621 cgctcggcgt gcttgagaaa acgctgcatc aggtcgggcc ccatgacgcc gtcgacgtcg
     1681 gccggccagt tgtcgacgtc ggtcgtcgtc atgagctggc cgccctgggc gatgccggtg
     1741 accagcatcg gcttcaggtt ggcgcgggcc gcgtagatcg cggcggtgta gcccgccgga
     1801 cctgagccga ggatgaggac gcgggcgtgc tgcggtgggt tcgtcatgga gttcgaggag
     1861 gaaggcaagg gccggcaagc cgcgtcgcgc aagtctgaca cgagggcctg ccccgctggc
     1921 atcggcgctc cgaatggtga caatagcgcc ggcgccgcca gtgtgagatt ttcacgcagg
     1981 ccggcaggcc gcgtttgttg acactgaaag gtacctcgat ggcgatgctc tcgaatctcg
     2041 atctcatccg gcgcgtaccg ttgttctcgc tgctgaccaa cgatcaggcc cagggcatcg
     2101 ccgacagcgt cgtcaagcga cgcttccgcc gcggcgagat cgtcgtcgag cacggcaaga
     2161 agacgaacgc ccttttcatc ctgctcaccg gccgggcccg ggtgctgacc gccgactcgc
     2221 gcggccgcga ggtgatcctc gccgtcctgc agtcgggcga ctacgtcggc gagatgagcc
     2281 tgatcgacaa cgagccgcat tcggccaccg tgcgtgccga ggtgcagacc gacatgctga
     2341 tcctcggccg cgccgaattc gcgcgctgcc ttcccgagaa ctcgagcctg tcgtacgcga
     2401 tcatgcg
//
LOCUS       DASRWS010000529         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1917982, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000529 DASRWS010000000
VERSION     DASRWS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917982"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFF72_09970"
     CDS             <1..319
                     /locus_tag="VFF72_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93534.1"
                     /translation="VVGVGIGFPLYGPAYWGPYPYGYYNPYVAYPAPRVIVAQPAPAP
                     APPAAPDPIFYPKSGQSPAQVETDRRECNRWATTQPGTMADASIFQRATYACMEGRGY
                     TVR"
     gene            388..582
                     /locus_tag="VFF72_09975"
     CDS             388..582
                     /locus_tag="VFF72_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93535.1"
                     /translation="MRATVSGCRSQIYSSLEAQLKDAGPFAEPQRKVLAALGKKLGEL
                     QATVQAVVKNVDDAAALAAK"
     gene            complement(718..2202)
                     /locus_tag="VFF72_09980"
     CDS             complement(718..2202)
                     /locus_tag="VFF72_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZV93536.1"
                     /translation="MSNINPSRRRFLRTASVVSGSVGTAAAPFALNLASLGTAVGQTA
                     PDYKAIVCLFLYGGNDSSNMVLRTDAASFAEYTRMRNTGNDPIALLAPGTAPAGNATR
                     ASPARLGGVLPIAPKFTVSTENAANTYAVHPSMPEVQSLFASGRLAILANAGPLVVPL
                     TRTDYTSNSKPRPQALGSHNDQQSTWQALGPEGVKIGWGGHLGDMVASSNTNTVYTSI
                     SVSGNAVFSAGQTTFQYQVGNGGAAAIGGISGSLFNSAAAASTLRSIITADNSHLFGQ
                     EYTSIVNRSINAQATFQTAFAASTVAAPTQYFQPSTATNVTNGLAQQLQTVARVIGAR
                     SALGAKRQIFFVSMGGFDTHDNQNGNQADLLARISHALAYFDSALSNVGGADMRNNVT
                     LFTASDFGRTITTNGDGTDHGWGSHHFVMGGAVKGGEIYGRFPIFGLNNNQDSANNAY
                     LPFTSVDTIGSTLGRWFGVSDSNLDTVFPNLHNFPRDLGFLKTA"
     gene            complement(2222..>2566)
                     /locus_tag="VFF72_09985"
     CDS             complement(2222..>2566)
                     /locus_tag="VFF72_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93537.1"
                     /translation="YMNYIRSWTTLDATRDIQQDYSAEKALAETPDALVDRMNLLLFS
                     GAMPDALKTQISAAVASRVIPGPTYPSGTAASAVATTTPSNQAQIDAAKLDRVYIAVF
                     LSMASPDYVIQK"
BASE COUNT          387 a          808 c          949 g          422 t
ORIGIN      
        1 cgtcgtcggc gtcggcatcg gctttcctct ctacggaccg gcgtactggg ggccgtatcc
       61 ctacggctac tacaacccct acgtggccta tccggcgccg cgcgtcatcg tcgcacagcc
      121 ggcgccggcg cccgcaccgc ccgcggcgcc cgatccgatc ttctatccga agagcggcca
      181 gagccccgcg caggtcgaga ccgatcggcg cgagtgcaac cgctgggcga cgacgcagcc
      241 cggcaccatg gccgacgcga gcatcttcca gcgcgccacg tacgcctgca tggaaggacg
      301 cggctacacg gtccgctgac gtcccggcgc gactcgggtc gcgccggcgt cgcatcgccc
      361 gacgatcggc gctggacgac ggtccacgtg cgggctaccg tatccggttg ccgttcgcag
      421 atctacagct cgctcgaagc gcagctgaag gacgccgggc ccttcgccga gccgcagcgc
      481 aaggtgctgg ccgcgctcgg gaagaagctc ggcgagctgc aggcgacggt gcaggcggtc
      541 gtcaagaacg tcgacgacgc ggctgcgctg gccgcgaagt agccgcggcg cgtgccggtt
      601 cgacgtgcgg cgacggcgtc gtccgcgtcg aagtcgtccc cgccaatgaa gaagggcagc
      661 ctgagctgcc cctggtgaaa gacgccaacg ggacttttca gcgtcccgcg acgcggatca
      721 ggcggtcttc aggaaaccca ggtcgcgcgg gaagttgtgc aggttcggaa acacggtatc
      781 gaggttcgag tcgctgacgc cgaaccatcg gcccagggtg ctgccgatgg tgtcgaccga
      841 ggtgaacggc aggtaggcgt tgttcgccga gtcctggttg ttgttgagcc cgaagatcgg
      901 aaagcgcccg tagatctcgc cgcccttgac ggcgccgccc atcacgaagt ggtgcgagcc
      961 ccagccgtga tcggtgccgt cgccgttggt ggtgatcgtg cggccgaagt cggaggcggt
     1021 gaacagcgtc acgttgttgc gcatgtcggc gccgccgacg ttcgacagtg ccgaatcgaa
     1081 gtaggccagc gcatgcgaga tgcgagccag caggtcggcc tggttgccgt tctgattgtc
     1141 gtgggtgtcg aagccgccca tcgagacgaa gaagatctgc cgcttggcgc cgagcgcgct
     1201 gcgcgcgccg atgacgcggg cgacggtctg cagctgctgg gcgaggccgt tggtgacgtt
     1261 ggtcgccgtc gacggctgga agtactgcgt gggcgcggcc acggtggacg ccgcgaacgc
     1321 cgtctggaag gtggcctgtg cgttgatcga gcggttgacg atcgacgtgt attcctgccc
     1381 gaacaggtgc gaattgtcgg ccgtgatgat cgagcgcagc gtcgacgcgg ccgcggccga
     1441 attgaagagc gagccgctga tgccgccgat cgccgcggcg ccgccgttgc cgacctggta
     1501 ctggaacgtc gtctggccgg ccgagaagac ggcgttgccg gagaccgaga tgctcgtgta
     1561 gacggtattg gtgttgctgc tcgcgaccat gtcgccgagg tggccgcccc agccgatctt
     1621 cacgccctcg gggcccagcg cctgccaggt cgactgctgg tcgttgtgcg agccgagcgc
     1681 ctgcggccgc ggcttggagt tcgacgtgta gtcggtgcgc gtcagaggca cgacgagcgg
     1741 gccggcgttg gccaggatcg ccagccgacc cgacgcgaag aggctttgca cctccggcat
     1801 gctcgggtgc acggcgtacg tgttcgcggc gttctcggtc gacacggtga acttgggcgc
     1861 gatcggcagc acgccgccca ggcgggccgg cgaggcgcgg gtggcgttgc ctgccggcgc
     1921 ggttcccggc gcgagcaggg cgatcgggtc gttgccggtg ttgcgcatgc gggtgtattc
     1981 ggcgaacgac gcggcatcgg tgcgcagcac catgttcgac gagtcgttgc cgccgtagag
     2041 gaacaggcag acgatggcct tgtagtcggg cgccgtctgg ccgaccgccg tgccgaggct
     2101 ggccaggttc agcgcgaagg gcgcggcggc ggtgccgacc gagccggaga cgaccgacgc
     2161 cgtgcgcagg aagcggcgac gcgatggatt gatgttcgac atgtcgatat cccttggact
     2221 cttacttctg gatcacgtag tcgggcgacg ccatcgacag gaagacggcg atgtagaccc
     2281 ggtcgagctt ggcggcgtcg atctgcgcct ggttgctggg cgtcgtcgtc gcgaccgcgc
     2341 tggcggcggt gcccgacgga taggtcggcc ccggaatgac ccggctggcg acggccgcgg
     2401 aaatctgcgt cttcagggca tcgggcatcg ccccggaaaa gagcagcagg ttcatgcgat
     2461 cgaccagcgc gtcgggggtc tcggcgagtg ctttctcggc gctgtagtcc tgctggatgt
     2521 cgcgcgtcgc gtcgagcgtc gtccacgaac ggatgtagtt catgta
//
LOCUS       DASRWS010000530         8269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1368041, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000530 DASRWS010000000
VERSION     DASRWS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368041"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VFF72_09990"
     CDS             complement(<1..153)
                     /locus_tag="VFF72_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZV93538.1"
                     /translation="MSTSSQAEARIFCVANQKGGVGKTTTTVNLAAGLSRIGRRVLLV
                     DLDPQGN"
     gene            complement(150..818)
                     /gene="rsmG"
                     /locus_tag="VFF72_09995"
     CDS             complement(150..818)
                     /gene="rsmG"
                     /locus_tag="VFF72_09995"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZV93539.1"
                     /translation="MSPLAAANGEGEASLDRAADELSLTLLPGQRAQLLEYIDLLVKW
                     SQTYNLTSIRDRSGMLSHHVIDCLAVLPSMRREMMGLSAPRVMDAGSGAGLPGVVIGI
                     MEPSWAVTCVDSVGKKTAFVRHVAGSLGLRRVEAEHGRVEDLTKQFDVVVSRAFASLE
                     AFTSRTRSALAEQGKWLAMKGRVPQQEIDALPLGVKVFHVEQIKVPALAAERSLVWMK
                     IDDK"
     gene            complement(815..2770)
                     /gene="mnmG"
                     /locus_tag="VFF72_10000"
     CDS             complement(815..2770)
                     /gene="mnmG"
                     /locus_tag="VFF72_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZV93540.1"
                     /translation="MLYPRHFDVVVVGGGHAGTEAALAAARMGAATLLLTHSLETLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGAMAQAADEAGIHFRILNGSKGPAVRATRAQADRV
                     LYRHAIRRRLERQDNLWLFQGACEDLFLHGDRIAGVQTQFGVRFECTRVILTTGTFLD
                     GRIHVGLDSHPGGRMGDSPSLRLSSRLKELQLPQGRLKTGTPPRLDGRTIDFSRLVEQ
                     PGDGVEENQDDVSGIPVFSFLGRPEQHPRQVSCWMTETNPRTHAIIRGGFAQSPMFTG
                     KIEGVGPRYCPSIEDKVNRFADKDSHQIFLEPEGLTTTEVYPNGISTSLPFSVQMEAI
                     RSIAGLERADILRPGYAIEYDYFDPRELRATFETRSIAGLYFAGQINGTTGYEEAAAQ
                     GLHAGVNAALSVRGEPAWTPSRSEAYLGVLVDDLVTKRVTEPYRMFTSRAEYRLSLRE
                     DNADARLTEQGRRLGLVTDDRWAAFERKREAIARETSRLASVRAGPCAATEGRPALTR
                     DYGLLDLLKRPDADFDSVWRWAEVEDVPRETLRQALGRPLADQVIEQIEIASRYAGYI
                     DRQTDEVTRSRTAETTALPDDLRYEDVRALSHEARQVLARHRPETLGSASRLPGVTPA
                     AISLLLVHLKKRARGEESSAEMGRVGI"
     gene            complement(2829..3314)
                     /locus_tag="VFF72_10005"
     CDS             complement(2829..3314)
                     /locus_tag="VFF72_10005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV93541.1"
                     /translation="MSGMAGTVAPLTLRWRWCPFGDLSVRELQYIYSVRQRVFALEQQ
                     CIYLDVDGCDERAYHLAAWSSAQREPLAYARVLEPGAKYDDVSIGRVVTWGAGRGCGL
                     GRELMARAIEGARGVWPGRPIRISAQSRLERFYEGFGFASVGAAYLEDGILHTEMVLA
                     G"
     gene            complement(3311..4486)
                     /locus_tag="VFF72_10010"
     CDS             complement(3311..4486)
                     /locus_tag="VFF72_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93542.1"
                     /translation="MTPQLRAVMTVLRKELTDALRDRRTLLTVLVSSVLMGPLILVAI
                     SGLVASLEAQAEQREVYVAGLANAPTLRNFLERQTYTVKEAPADYEEALRKSRFTDPV
                     VVVHRDFETELARGESPVVEIVSDSANQRSEASAGRIERLLGGFARERVVLNLAMRGV
                     AVQLLEPIDVESRDLASPQTRATRITGMLPYFVMMAVLYGALNAALDTTAGERERGSL
                     EPLLMNPVERWALVIGKWAAVAGVAMLIAVLSSFSFLPGQWVLRSDTLAALFQYGPRE
                     AFYFLVVLVPFAAALSAVLMAVAIRCRTFKEAQASATIVLLATSLLPLINIFNLGSDA
                     PWHLWIPALAQNTLMMRVLKGEGFGVADILIPLTVCVVLTLGGIGFVARMLRHAAMK"
     gene            complement(4483..5298)
                     /locus_tag="VFF72_10015"
     CDS             complement(4483..5298)
                     /locus_tag="VFF72_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93543.1"
                     /translation="MIEVSGLAKSFVVAEAGRRRGLAALVPRRGRGGRVVHAVRDVSL
                     HAVDGRITGLLGPNGAGKTTTLRMLAGLLTPDAGQLRVDGIDVVARPREVLARMGMLS
                     DSRGLYPRLTARENIVYYGTLQGMTRDAADARAVELARMFAMTTLLDRRTDGFSQGER
                     MKTALVRALVHDPSNIVLDEPTNGLDVIATRALRENLRWLRSAAGGGKCIVFSTHIMQ
                     EVERLCDSVVVVADGRAVASGTVPELLERTGETDFEEAFVKLAFPAETWRPAA"
     gene            complement(5295..6824)
                     /locus_tag="VFF72_10020"
     CDS             complement(5295..6824)
                     /locus_tag="VFF72_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV93544.1"
                     /translation="MSRLLGRGGAAIVLAVAGLLGAFAVRADGPVPALADCHVAGIVN
                     GVLCGSMLRPLDPDDPQRGSITVRYVVVPALARRKLADPVFMLAGGPGQSAIAVAAQT
                     MPLFSRLNNRRDIVFVDQRGTGGSAPLLCPNEANESLAEQSDPERQVRLMQQCKARLL
                     TLPYIHGEADLGFFTTWIAVRDLDAVRRRLGAGQIDLVGASYGTRVGLEYLRQFPQSV
                     RRIVLDGVAPPYMSLPTSFSTDNQAALDKLMAACRAEAACSRAHPRLQANLAALLAGL
                     PKGVDAAPPLTGRVEHFILTHDMVLDALRGALYVPALAAALPDAIDAAAAGRYDGLLG
                     LDATLFSKKSTRLATGMNLSVVCAEDVPRIALSLAVDPPGTTFGDSFASLYEKLCAVW
                     PRGPVPAAFYSVGPTRSPALLLSGGIDPVTPPRHAEQVARELGPMARQVIVANAGHGV
                     MSIGCMRDVVYRFIDAADDAGALRVDAACAAHVPRPPAFVSVGDAVAAASAAASGSSA
                     R"
     gene            complement(6821..7189)
                     /locus_tag="VFF72_10025"
     CDS             complement(6821..7189)
                     /locus_tag="VFF72_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZV93545.1"
                     /translation="MARTILEEARQHPAIRERIATLNADIVHNVQAAATTNPVLVVGM
                     AGNPHVRRARKALAAAGVASHYLEYGSYFSEWRKRNALKMWTGWPTFPMIFVKGTLIG
                     GADQIDALIASGELKRTLGE"
     gene            complement(7258..8142)
                     /locus_tag="VFF72_10030"
     CDS             complement(7258..8142)
                     /locus_tag="VFF72_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZV93546.1"
                     /translation="MNISASTFRSRGVERLVRGQATSDGDGVKLTRVLTQGLQQRLDP
                     FLMLDAFGSDEAVDYIGGFPDHPHRGFETVTIMQEGRMRHHDSVGNVGLLEPGSVQWM
                     TAGRGIIHSEMPEQERGRMAGFQLWVNLAARDKMQPPAYRDIAPADVPDLRIEDGARV
                     RVIAGSCDGVEGAVTRPVTEPVVLDVTLPAGAVFDAVLPPGHNAFAYVSTGSVDVGSG
                     SLARVDAGVMAILENDALATGVRLQAAADLPAPARILLVAGRPLGEPIAQYGPFVMNT
                     TAELQRAVSDFQRGVLAG"
BASE COUNT         1425 a         2852 c         2695 g         1297 t
ORIGIN      
        1 gttgccctgc ggatcgaggt cgacgagcaa aacgcggcgg ccgattcgcg agagacccgc
       61 ggcgagattg acggtcgtcg tcgtctttcc tacgccgccc ttttgattgg caacacagaa
      121 aatcctcgcc tcggcctgag agcttgtact cacttatcgt cgatcttcat ccagactagc
      181 gagcgttcgg ccgccaaggc ggggaccttg atttgttcca cgtgaaacac cttgacgccc
      241 agcggcaggg catcgatttc ctgctgcggc acgcggccct tcatggccag ccacttcccc
      301 tgttccgcca gcgccgagcg tgttcgactg gtaaaggctt cgagggaagc gaaagcccgc
      361 gacacgacca cgtcgaactg tttggtcaaa tcctcgaccc gtccatgctc ggcctcgacg
      421 cgccgcagtc cgagagagcc ggcgacgtgc ctgacgaacg ccgtcttctt tccgacgctg
      481 tcgacgcacg tcaccgccca tgacggctcc atgatgccga tcacaacgcc cggcaacccg
      541 gcaccgctgc cggcgtccat cactcggggc gccgacaaac ccatcatctc tcgccgcatc
      601 gacggcaata ccgccaggca gtcgatgaca tgatgcgaga gcatcccgga gcggtctcgg
      661 atcgaggtca ggttgtacgt ttggctccac ttcaccagca gatcgatgta ctcgagcagc
      721 tgtgctcgtt gccccggcag cagcgtcaat gaaagctcgt cggcagcgcg atccagcgac
      781 gcctcgcctt cgccattggc ggcggccagc ggactcaaat acccacccga cccatctcgg
      841 cgctcgactc ctcgcccctg gctcgcttct tgaggtgcac gagcaacagc gaaatggccg
      901 cgggagtcac tccgggcagg cgtgacgccg agccgagcgt ttccggccga tgcctcgcaa
      961 ggacctgccg ggcttcgtgc gaaagcgccc tcacgtcctc gtatcgaagg tcgtcaggca
     1021 gcgcggtcgt ctccgccgtg cgtgacctcg tcacctcgtc cgtctgccgg tcgatatatc
     1081 cggcgtagcg cgaagcgatc tcgatctgct cgatgacctg gtcggccagg gggcgcccga
     1141 gcgcctggcg caacgtttca cgtggaacat cctcaacctc cgcccagcgc cagacggaat
     1201 cgaagtcggc gtcggggcgt ttgaggagat cgagcaaacc gtagtcgcgc gtgagcgcgg
     1261 gtcgaccctc ggtggccgca caagggcccg cacgaacgga cgcgagccgc gaggtttcgc
     1321 gggcgatggc ctcgcgcttt cgctcgaacg cggcccagcg atcgtccgtg acgaggccca
     1381 gtcgcctgcc ctgttcggtc agccgggcgt cggcgttgtc ctcgcgaagc gacagccgat
     1441 attcagcacg gctggtgaac atccggtacg gttcggtgac gcgcttcgtc accagatcgt
     1501 ccaccagcac gccgaggtag gcctcgcttc gggatggcgt ccaggcgggc tctcccctca
     1561 ctgagagtgc cgcgttgacg cccgcatgca ggccttgcgc agccgcctcc tcgtaccccg
     1621 tcgtgccatt gatctggccc gcaaaataga ggcccgcgat cgaccgcgtc tcgaatgtcg
     1681 cccgtaactc ccgcggatcg aaatagtcgt attcgatggc atagcccggc cgcaggatgt
     1741 cagcgcgctc caggccggcg atcgagcgga tcgcctccat ctgaaccgaa aacggcagcg
     1801 acgtcgaaat gccgttcgga tacacctctg tggttgtcag accttcgggc tccaggaaga
     1861 tctgatgcga atccttgtcc gcgaatcgat tgaccttgtc ctcgatgctc gggcagtagc
     1921 gggggccaac cccttcgatc tttcccgtga acatggggct ttgcgcgaaa ccgccccgga
     1981 tgatcgcgtg cgtgcgagga ttggtttcgg tcatccagca ggagacttgc cgggggtgct
     2041 gttccggccg gccgagaaag ctgaataccg ggataccgga aacgtcgtcc tggttttcct
     2101 cgacaccgtc gccgggctgc tccaccagcc gcgagaagtc gatggtgcgc ccgtcgaggc
     2161 gaggcggagt tcccgtcttg agacgcccct ggggaagttg cagctctttc agacgtgaag
     2221 aaaggcgcag cgacggggaa tcgcccatgc gcccgcctgg atggctgtcg aggccgacgt
     2281 ggatgcgccc gtccaggaag gtgccggtcg tcaggatgac ccgggtgcac tcgaaccgca
     2341 caccgaactg ggtctgaacg ccggcgatcc tgtcaccgtg aagaaacagg tcttcgcagg
     2401 cgccctggaa cagccacagg ttgtcctgtc gctcgagtcg cctccggatg gcatgtctgt
     2461 aaagcacccg gtcggcttgc gcccgcgtgg cgcgaacggc cggccccttc gaaccgttca
     2521 aaatgcgaaa gtgaatgccg gcttcatcgg cagcctgcgc catggcgcca cccagcgcat
     2581 cgatctcctt gacaagatgg cccttgccaa tgccgccgat cgacggattg caactcatct
     2641 gcccgagagt ttcgagcgaa tgagtcagca gcaatgtggc tgcacccatc ctcgccgcag
     2701 caagcgcggc ttcggtgccg gcatgcccac cgccgacgac gacaacatca aagtgcctgg
     2761 gatagagcat cactggccct ggctgcaaag cctgcgattg tatttttcag cacaggaaag
     2821 tcctcgcctc acccggcgag gaccatttcc gtgtgcagga tgccgtcttc gaggtaagcc
     2881 gcgccgaccg acgcgaagcc gaacccctca tagaaccgct ccaaccgcga ctgcgccgag
     2941 atgcggatcg gccgacccgg ccagacgccc cgtgccccct cgatggcccg cgccatcagc
     3001 tcgcgcccga gaccgcaacc ccgtccggct ccccacgtga cgacccgacc gatcgagaca
     3061 tcgtcgtact tcgcgcccgg ctcgagaacg cgcgcgtagg ccagcggctc gcgctgcgcc
     3121 gacgaccacg ccgccaggtg gtacgcccgt tcgtcgcagc cgtccacatc gagatagatg
     3181 cactgctgct cgagcgcgaa cacgcgctga cgcaccgaat agatgtactg caattcacgc
     3241 acgctcaagt cgccgaacgg acaccaacgc catcgcagcg tcaatggcgc caccgtgccc
     3301 gccatcccac tcatttcatc gccgcatgcc gcagcattcg ggccacgaac ccgatgccac
     3361 ccagggtcag caccacgcat accgtgagag gaatgaggat gtccgcgacg ccgaatcctt
     3421 cgcccttgag gactcgcatc atcaaggtgt tctgcgccag tgccgggatc caaaggtgcc
     3481 agggcgcgtc gctgccgagg ttgaagatat tgatcagcgg caggagcgac gtcgccagaa
     3541 gcacgatggt cgcgctcgcc tgcgcctcct tgaaggtccg gcatcggatc gccaccgcca
     3601 tcagcacggc cgacagcgcg gcggcgaaag ggaccagcac caccaggaaa tagaaggcct
     3661 cgcgcggtcc gtactggaac aaggccgcca gcgtgtcgct gcgcaacacc cactgtcccg
     3721 gcaggaagct gaaggagctg aggacggcaa tcaacatcgc cacccctgcc accgccgccc
     3781 acttgccgat caccagcgcc cagcgctcga ccgggttcat gagcagcggc tcgagcgagc
     3841 cacgttcgcg ttcgccggcg gtcgtgtcca gcgcggcatt gagcgctcca tagagcaccg
     3901 ccatcatgac gaagtagggc agcatgccgg tgatccgggt ggcgcgggtc tgcggacttg
     3961 ccaggtcgcg gctttcgacg tcgatcggct cgagaagctg aacggccacg ccgcgcatgg
     4021 cgaggttgag caccacgcgc tcgcgagcga atccgcccag cagccgctcg atccgcccgg
     4081 cgctggcctc ggaacgctgg ttcgcgctgt ccgagacgat ctcgacgaca ggcgactcgc
     4141 ccctcgccag ctccgtctcg aaatcacgat gaacgaccac caccggatcg gtgaagcgcg
     4201 acttgcgcag cgcctcttcg tagtccgccg gtgcctcctt gacggtgtac gtctgtcgtt
     4261 cgagaaagtt gcgaagcgtt ggcgcgttcg cgagacccgc gacgtagacc tctcgctgct
     4321 cggcctgcgc ttcgagcgac gccacgagcc ccgagatcgc gacgaggatg agcggcccca
     4381 tcaacaccga cgagaccagg accgtgagca gcgtccggcg atcgcgcagg gcatccgtca
     4441 gctccttgcg caagaccgtc atgacggcgc gaagttgtgg cgtcatgccg cgggccgcca
     4501 ggtctcggcc gggaaggcca gcttcacgaa cgcctcctcg aaatcggttt caccggttcg
     4561 ttcgagcaac tcgggcacgg tgccgctggc gactgcgcga ccgtcggcga ccaccacgac
     4621 gctgtcgcac agccgctcga cctcctgcat gatgtgcgtc gagaaaacga tgcatttgcc
     4681 gccgccggcg gccgatcgca gccagcgaag gttttcgcgc aaggcccgcg tcgcgatgac
     4741 gtcgaggccg ttcgtcggct cgtcgagaac gatgttcgac gggtcgtgga ccagcgcccg
     4801 cacgagcgcc gtcttcatcc gctctccttg gctgaatccg tcggttcgac gatcgaggag
     4861 cgtggtcatc gcgaacattc tcgccagctc gacggcccgc gcgtcggccg cgtcgcgcgt
     4921 catgccttga agggtgccgt aatagacgat gttttcccgc gcggtgaggc gcggatacag
     4981 gccgcgcgaa tcggacagca tccccattct cgccagcacc tcgcggggcc gagcgaccac
     5041 atcgatgcca tcgacgcgca gctgccctgc gtccggcgtg aggagcccgg ccagcatccg
     5101 cagcgtcgtt gtcttgccgg cgccgttcgg gccgaggagg ccggtgatgc ggccgtcgac
     5161 cgcatgcagg ctgacatcgc gaacggcatg caccacccgg ccgccccggc cacgccgggg
     5221 tacgagcgcc gcgagacccc tccgacggcc agcctcggcg acgacgaagc tcttggcgag
     5281 cccgctgact tcgatcatcg cgcggacgaa cccgatgcag ccgccgacgc cgcggcgacg
     5341 gcgtcaccga cgctgacgaa ggccggggga cgcggcacgt gggccgcgca ggcggcgtcc
     5401 acgcgcaacg cgccggcgtc gtcggcggcg tcgatgaagc ggtacacgac gtcgcgcatg
     5461 cagccgatcg acatcacgcc gtggcccgcg ttggcaacga tgacctgccg tgccatcggc
     5521 ccgagctcgc gcgcaacctg ctccgcatgg cgcggcggcg tcaccggatc gatgccgccg
     5581 ctcaggagca gcgccggcga acgcgtcggc ccgaccgagt agaacgccgc cggcaccggt
     5641 ccccgcggcc agacggcgca cagcttctcg tagagcgacg caaacgaatc gccgaaggtc
     5701 gtgccgggcg ggtcgacggc aagcgagagc gcgatccgcg gcacatcttc ggcgcagacc
     5761 accgacaggt tcatgccggt cgcaagccgg gtcgacttct tcgagaacag cgtcgcgtcg
     5821 agcccgagca agccgtcgta gcggccggcg gccgcggcgt cgatcgcatc gggaagcgcc
     5881 gccgccagcg ccggcacgta gagcgcgccg cgaagcgcgt ccaggaccat gtcgtgcgtc
     5941 aggatgaagt gctcgacgcg acccgtcagc ggcggcgccg cgtcgacacc cttcggcagg
     6001 ccggcgagca gcgccgcgag attcgcctgc agtcggggat gggcgcgtga acatgccgct
     6061 tcggcccggc aagcggccat gagcttgtcg agcgccgcct ggttgtcggt cgaaaagctc
     6121 gtcggcaagg acatgtacgg cggcgcgaca ccgtcgagga cgatgcggcg gaccgattgc
     6181 gggaactggc gcaggtattc gaggccgacg cgtgttccgt aggaagcgcc gacgagatcg
     6241 atctgcccgg caccgagtcg gcgacgcacc gcatcgagat cgcgaacggc gatccatgtc
     6301 gtgaaaaacc ccagatcggc ttctccatgg atgtacggaa gcgtcagcaa ccgggccttg
     6361 cactgctgca tcaggcgaac ctgccgctcc ggatcggact gctcggcgag ggattcgttg
     6421 gcctcgttcg ggcacaggag gggcgccgag ccgccggtgc cgcgctggtc gacgaagacg
     6481 atgtcgcgcc gattgttgag cctggaaaac agcggcatcg tctgcgccgc aacggcgatg
     6541 gcgctctgtc ccgggccgcc ggccagcatg aagacgggat cggcgagctt gcgacgcgcc
     6601 agggcgggga cgaccacgta gcgaaccgtg atcgagccgc gctgcggatc gtcggggtcg
     6661 agcggccgca gcatgctgcc gcacagcacg ccgttgacga ttccggcgac atggcaatcc
     6721 gccagcgccg ggacggggcc gtcggcgcgc accgcgaatg caccgagcag gccggccaca
     6781 gcgagcacga tcgccgcgcc gccgcgaccg agtaggcggc tcattcgccg agcgttcgct
     6841 tcagttcgcc gctggcgatg agggcgtcga tctgatcggc accgccgatc agcgtgccct
     6901 tgacgaagat catcgggaac gtcggccatc cggtccacat cttcagcgca ttgcgctttc
     6961 gccattcact gaagtagctc ccgtactcca gatagtgact ggccacgccc gcggccgcca
     7021 gcgccttgcg cgcccggcgg acgtgcggat tgccggccat gcccacgacg agcaccgggt
     7081 tcgtcgtcgc cgccgcctgc acgttgtgca cgatgtcggc gttgagggtg gcgatccgct
     7141 cccggatcgc cgggtgctgg cgggcttctt cgaggatcgt gcgggccatg gtgttcgcct
     7201 tgcggatgcg gcaggcggcg accataccac ccgccggcgg ctcggcgggc cgccccttca
     7261 cccggcgagg acgcctctct ggaagtcgct gaccgcgcgt tgcaactcgg ccgtcgtgtt
     7321 catgacgaag gggccgtact gggcgatcgg ctcgcccagg ggccgccccg ccaccagcag
     7381 gatcctggcg ggtgccggca gatcggccgc cgcctgcaat cgcacgccgg ttgcaagggc
     7441 gtcgttttcg agaatggcca tgacaccagc atcgacgcgc gccaacgacc cgctgccgac
     7501 gtccacgctg ccggtcgaga cgtacgcgaa ggcgttgtgg cccggcggca gcaccgcatc
     7561 gaagaccgcg cccgccggca aggtcacatc gagcacgacg ggctcggtga ccggccgcgt
     7621 gacggcgccc tcgacgccat cgcagcttcc ggcaatgacg cgaacgcgag cgccatcttc
     7681 aatgcgcaga tctggaacgt ccgcgggcgc gatgtcgcgg taggcgggcg gctgcatctt
     7741 gtcgcgcgcc gcgaggttga cccagagctg gaatccggcc atccgaccgc gttcctgctc
     7801 gggcatctcg gagtggatga tgccgcgccc ggcggtcatc cactggacgc tgcccggctc
     7861 cagcaggccg acgttgccga cgctgtcgtg gtggcgcatg cggccttcct gcatgatcgt
     7921 caccgtctcg aaaccgcggt gcgggtgatc cggaaagccg ccgatgtagt cgaccgcctc
     7981 gtcgctgccg aaggcgtcga gcatcaggaa ggggtcgagg cgctgctgca ggccttgcgt
     8041 gaggacccga gtgagcttga cgccgtcgcc gtcggacgtc gcctggccac ggacgaggcg
     8101 ctcgacgccg cgcgagcgga aagtggatgc agagatgttc atggaggcaa tgtagcgacg
     8161 ccgggccttt cggcaagctt gccgcgtttg cacgccgcgt tcaatccgcg cgaatgcgac
     8221 gtttcgacgt ccggggtgta ataagggccc tctcgctcac gacctcact
//
LOCUS       DASRWS010000531         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3093033, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000531 DASRWS010000000
VERSION     DASRWS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093033"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3041)
                     /locus_tag="VFF72_10035"
     CDS             complement(<1..>3041)
                     /locus_tag="VFF72_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93547.1"
                     /translation="IYRYIGPTLIDSDPTQDGNQPFDLTIQQYQDSTLWQQVNVSEEP
                     AQVRAFVENSSIDAAGDLSITANAHEEIHSVVVAFAAGVGGGSDAGFALSAAGVYAEN
                     KIRTDIKADIDGDGTTGISAASVEVVATDASSIDAIAGAASLAAGFGADVGVAVSIGL
                     SLGFNEVENDVQAFADNADNGITTFAGGVTISSATQGRHLFDLSNLGALTPDDLDNAA
                     TAAPAGTDDPDDPSKPDSDPNDPDDQQGIDFRNDAIHDQAVLAALRAAMDAQLAQHGE
                     SPLSVQDTVGTESMFTTDSGRLAVAAVVGNDQLVFNADDGFHTGDAVIFENDGGEDIG
                     LANGTYYVIVVDRRTIELAKTKDEATAQTPVPIETLVQPADGLGGQSVRPLFEVGQGT
                     TVKLADGYAFGGDAGRVYEYIGPPTGDTDEPVTLDLATQDYTSSSWLEVDKLKISVVQ
                     PGQSWELISPDGTTYALELTGTGPLTLSVGKNTINAISAAASMALGLGEVGAAISGAG
                     AVAQNVILGSTQAYAQDSKLTSASDVSLSATSTSDISSTVLALSAAAGVGNVGVGASI
                     GIAVAENFIGWQPGANDATPIEVQSYLQDTPVHAGGNLSLTALADQGIDSFVLAGSVA
                     IGAGVAAGVAASGSGVFSENRIGIDVGSDILGSGLTPTDAASVTLDAEDTSLITAFAG
                     AISVAAALGGNLGLGVSIGVSIANNTIDGQVEAVIDGADVHTTSGGVSVTATDAATID
                     SVAAAASVAIGIAVDLGVGVAVSGAGALAENTIFGGTTAEISDSNVVSAGDVIVSAAD
                     TSHIQGTIVSASVAVGGALLGAVGASIGMGLARNIIGGALDTDPNDFGSAILSTANVL
                     TLHPGDEVRVASGPRTGDVYQYLGTTDFHATYDFTTADTPDQVQTGQRVFVPAAGGAD
                     DAVYQYVGKDPLDKPDLSPTAQNYSDTGKWQRVLFTTASDPALVKAGDQVEVTGGDGT
                     ISVYQYVGKDALASLTDQDYTNTAKWRQITAPLEQDYGNPE"
BASE COUNT          459 a         1022 c         1005 g          555 t
ORIGIN      
        1 gttccgggtt gccgtagtcc tgttcgagcg gcgccgtgat ctgccgccat ttggcggtgt
       61 tggtgtagtc ctgatcggtc agactagcga gcgcatcttt gcccacgtac tggtagacgc
      121 tgatcgttcc atcgccgccg gtgacctcga cttggtcgcc ggccttgacc agcgccggat
      181 cgctggcggt cgtgaacagc acccgctgcc acttcccggt gtcgctgtag ttctgtgcgg
      241 tcgggctcag gtccggttta tcgagcggat ccttccccac atactgatag acggcatcat
      301 cggccccgcc ggcggcagga acgaagacgc gctggccggt ctgaacctgg tccggcgtgt
      361 cggcggtggt gaagtcgtag gtcgcgtgga aatcggtggt cccgagatat tggtacacgt
      421 cgccggtgcg cgggccgctg gcgacgcgga cctcgtcgcc cgggtgcagc gtcagcacgt
      481 tggcggtcga gaggatagcg gagccgaaat cgttgggatc ggtatcaagc gcgccgccga
      541 tgatgttgcg cgccagtccc atgccgatcg acgcgccgac ggcacccaac agggccccgc
      601 cgacggcgac cgaggccgaa acgatggtgc cttggatgtg cgaggtgtcc gctgcactga
      661 caatgacatc tccggcgctc acgacattgc tgtccgagat ttccgccgtg gtgccgccaa
      721 agatcgtgtt ctcggccaac gcccccgcgc cgctcacggc gacgcctacc cccaggtcga
      781 cggctattcc gatcgcgacc gacgcagccg cagcgaccga gtctatggta gcggcgtccg
      841 tcgccgtcac gctgacgccg cccgatgtcg tatgcacatc ggcgccgtcg atgacggcct
      901 cgacctggcc atcgatcgtg ttgttcgcga tcgacacgcc aatggagacc cccaaaccga
      961 ggtttccgcc gagtgcggcg gcgaccgata tggcgcccgc gaacgcggtg atgagcgaag
     1021 tgtcctccgc atccagcgtc acgctggccg cgtcggtagg tgtaagaccc gagccgagaa
     1081 tgtcgctgcc gacgtcgatg ccaatgcgat tctcggagaa gacgcccgag ccgctcgcgg
     1141 cgactccggc agccacaccg gccccgatcg ccaccgagcc cgccaggacg aacgagtcga
     1201 tgccctgatc ggcgagcgcc gtcagcgaca ggttgccgcc ggcatgcacc ggcgtgtcct
     1261 gcagatagga ttgcacttca atgggtgtcg catcattggc acccggctgc catccgatga
     1321 aattctccgc caccgctatg ccgatggacg cgccaacgcc gacgttgccg acgccggcgg
     1381 ccgccgacag ggccaatacg gtcgacgaga tgtcggatgt gctcgtcgca ctcagtgata
     1441 cgtcgctggc gctcgtgagc ttgctgtcct gcgcatatgc ctgcgtgctg cccaggatca
     1501 cattctgcgc cacggcgccg gcgccgctga tggccgcgcc aacctcgccg aggcccagcg
     1561 ccatcgaggc tgccgccgag atcgcgttga tcgtgttctt ccccacgctc agcgtcagcg
     1621 ggcccgtgcc cgtgagctcg agcgcgtagg tcgtgccgtc cggcgagatc aactcccagc
     1681 tctggccggg ctgcaccacc gagatcttga gcttatcgac ctcgagccag ctcgaggacg
     1741 tgtagtcttg cgtcgccaga tcgagcgtga ccggctcatc cgtgtcgccg gtcggggggc
     1801 cgatgtactc gtagacgcga ccggcgtcgc cgccgaacgc atagccatcg gcgagcttca
     1861 ccgtggtgcc ctggcctacc tcgaacaggg gccggacgga ctgcccgcca agtccgtcgg
     1921 ccggctgcac cagcgtttcg atcgggactg gcgtctgcgc cgtcgcttca tctttcgttt
     1981 tcgcaagctc gatcgtccgc cggtcgacca cgatgacgta gtacgtgccg ttcgcgagcc
     2041 cgatgtcctc gccgccatcg ttctcgaaga tcaccgcatc gccggtatgg aagccatcgt
     2101 cggcattgaa aaccagctga tcgttgccga cgacggcggc cacggcgagc cggccggagt
     2161 cggtggtgaa catcgactcg gttcccaccg tatcctgcac cgagagcggg ctctccccgt
     2221 gctgcgcgag ctgcgcgtcc atggctgcgc gcagcgcggc gaggacggcc tgatcgtgga
     2281 tcgcatcgtt cctgaagtcg atcccctgct ggtcgtccgg gtcgttcggg tcgctgtccg
     2341 gcttgctcgg gtcgtccgga tcatccgtgc cggcgggcgc ggccgtggcc gcattgtcca
     2401 ggtcgtcggg ggtcaatgcg ccgaggttgc tcaggtcgaa cagatgccgg ccctgcgtgg
     2461 ccgacgagat ggtgacgccg ccggcgaagg tcgtgatgcc gttgtcggca ttgtcggcga
     2521 acgcctgcac gtcgttctcg acttcgttga acccgagcga caggccgatc gacaccgcca
     2581 caccgacgtc cgcgccgaac ccggcggcca gcgaagccgc gcccgcaatg gcatcgatgc
     2641 tagacgcatc ggtcgccacg acctccacgc tcgcggcgct gatgccggtc gtgccgtcgc
     2701 cgtcgatatc ggccttgatg tcggtcctga tcttgttttc cgcgtagaca ccggccgcgc
     2761 tcagcgcgaa tccggcgtcg ctgccgccgc cgactccggc cgcgaaggcg acgacgacgg
     2821 aatggatttc ctcgtgtgcg ttggcggtga tgctgaggtc gccggccgca tcgatgctcg
     2881 agttctcgac gaaggcgcgg acctgcgcgg gctcctccga gacattgacc tgttgccaca
     2941 gggtcgagtc ctggtactgc tggatcgtca ggtcgaaggg ctggttgccg tcctgggtcg
     3001 ggtcgctgtc gatcagcgtc ggcccgatat atcggtagat g
//
LOCUS       DASRWS010000532         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3665032, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000532 DASRWS010000000
VERSION     DASRWS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665032"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..697)
                     /locus_tag="VFF72_10040"
     CDS             complement(158..697)
                     /locus_tag="VFF72_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HZV93548.1"
                     /translation="MSTAAFPARPPARKGKAGIVSSSHLVSPRSVELSELEFGLIIAW
                     NAFSRWAMRCMAAAGCAELTITDVLVLHHVCHRARNKKLGDICFVLNIEDTHVVAYSL
                     KKLLSAGLARGDKSGKEVFYSPTPAGETAVTKYREVREACLIANLDGDRNADIGEAAR
                     LLRTMSGLYDQAARAATSL"
     gene            844..1839
                     /locus_tag="VFF72_10045"
     CDS             844..1839
                     /locus_tag="VFF72_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZV93549.1"
                     /translation="MGSIARSLRATARSVAVLALAGAAFGVAAQTKWDLPAAYPATNF
                     HTENLQHFADDVDKATGGKLKITVHANASLFKANEIKRAVQGDQAQAGEILLTAYSNE
                     WPIFGIDGLPFLADSYDEAWKLYQAEKPALEKRLASQGMMLLYTVPWPPQGIYVNKPI
                     HSAADLKGVKWRAYSPATARIAELVGAQPVTVQAAELSQAMATGVVESYMSSGSTGYD
                     TKTYEHIKYWYDTQAWLPKNAVIMNKSAFDALDKTTQAAVLKAAAEAEKRGWALSKQK
                     NGEYIELLKKNGMHIETPSPQLKADMKKVGDVMLKEWLQKAGPEGQEIVDAYKKM"
     gene            1854..>2283
                     /locus_tag="VFF72_10050"
     CDS             1854..>2283
                     /locus_tag="VFF72_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZV93550.1"
                     /translation="MPATAHPPARGPVGRALDRLYDATAALGAAAMVLLLGAVLLSIV
                     AREIGFDVPGIDAYAGYLMAATGFLAMAHTLKRGEHIRVTLLLSALHGPARRGIEIWA
                     LFAATLLATLLAVYTCKLAWQSHTYHDISTANDATPLWLPQ"
BASE COUNT          409 a          807 c          727 g          340 t
ORIGIN      
        1 ccgatgtcgc cgaggggccg cgggaaacgc gtgtcgagca tgatgacgcc gagcaccggt
       61 cgagtgtctc gcgacagcgg cgtcgccgcg tccgccgcgt cgccgtgaga cgcaacacct
      121 gccactggcg cagccggctg cgtcacagca ggcatcgtca cagcgaggtc gcggcgcggg
      181 ccgcctggtc gtagaggccc gacatcgtgc gcagcaggcg cgccgcttcg ccgatgtcgg
      241 cattgcggtc gccgtcgagg ttggcgatca ggcaggcctc gcgcacctcg cggtatttcg
      301 tcaccgccgt ctccccggca ggcgtcgggc tgtagaagac ttccttgccc gacttgtcgc
      361 cgcgcgccag gcccgcggag agcagcttct tgagcgaata ggcgacgaca tgcgtgtcct
      421 cgatgttgag cacgaagcag atgtcgccga gcttcttgtt gcgggcccga tggcagacgt
      481 gatgcaggac gaggacgtcg gtgatggtga gctcggcgca accggccgcc gccatgcagc
      541 gcatcgccca gcgcgagaag gcgttccagg caatgatcag cccgaactcg agctccgaca
      601 gctcgacgct cctgggcgag acgagatgcg acgacgagac gatgccggcc ttgcccttgc
      661 gggccggcgg gcgcgccgga aacgcggccg tcgacattcg gtgattccct cgattcgttg
      721 aaaacttatt gacaatttat cgaaatactt tctagactgc aagcgctcgg gaagaaaccg
      781 atgccgtgcc cccgaggcac gacctatgct ttgcccccct gatccacgca cgtcggagta
      841 cccatgggtt ccatcgctcg ctctcttcgc gccacggcgc gcagcgtcgc cgtccttgcc
      901 ctcgccggcg ccgcctttgg cgttgctgcc cagaccaagt gggatcttcc ggccgcctac
      961 ccggcgacga acttccacac cgagaacctg cagcatttcg ccgacgacgt cgacaaggcg
     1021 accggcggca agctgaagat caccgtgcac gccaacgcgt cgctcttcaa ggccaacgag
     1081 atcaagcgcg ccgtccaggg cgaccaggca caggccggcg agatcctcct caccgcctat
     1141 tccaacgagt ggccgatctt cggcatcgac ggactgccct tcctcgccga cagctacgac
     1201 gaggcctgga agctctacca ggccgagaag ccggcgctcg aaaagcgcct cgccagccag
     1261 ggaatgatgc tgctctacac ggtgccgtgg ccaccgcagg gcatctacgt caacaagccg
     1321 atccattcgg ccgccgacct gaagggcgtc aagtggcgcg cctacagccc ggcgacggcg
     1381 cgcatcgccg agctcgtcgg cgcccagccg gtcaccgtgc aggccgccga gctgtcgcag
     1441 gccatggcca ccggcgtcgt cgaaagctac atgtcgtcgg gctcgaccgg ctacgacacc
     1501 aagacctacg agcacatcaa gtactggtac gacacccagg cctggctgcc gaagaacgcc
     1561 gtcatcatga acaagtcggc cttcgacgcg ctcgacaaga cgacccaggc cgccgtcctg
     1621 aaggccgcgg ccgaagccga gaagcgcggc tgggccctgt cgaagcagaa gaacggcgag
     1681 tacatcgagc tgctgaagaa gaacggcatg cacatcgaga cgccttcgcc gcagctcaag
     1741 gccgacatga agaaggtcgg cgacgtcatg ctgaaggaat ggctgcagaa ggccggcccc
     1801 gagggccagg aaatcgtcga cgcctacaag aagatgtgat cgcgcgcgag ccgatgcccg
     1861 cgactgccca tcctcccgcg cgcggcccgg tcggccgcgc cctcgaccgc ctctacgacg
     1921 ccaccgcggc gctcggcgca gcggcgatgg tgctgctgct cggcgccgtg ctgctgtcga
     1981 tcgtcgcgcg cgagatcggc ttcgacgtcc ccgggatcga cgcctacgcc ggctacctca
     2041 tggcggccac cggcttcctg gcgatggcgc atacgctcaa gcgcggcgag cacatccgcg
     2101 tcacgctcct cctctcggcg ctgcatggcc cggcgcggcg tggaatcgag atctgggcgc
     2161 tgttcgcggc gacgctgctg gccacgctcc tcgccgtcta cacctgcaag ctcgcgtggc
     2221 agtcgcacac gtaccacgac atctcgaccg ccaacgacgc gacgccgctc tggctcccgc
     2281 aga
//
LOCUS       DASRWS010000533         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3665539, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000533 DASRWS010000000
VERSION     DASRWS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665539"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFF72_10055"
     CDS             <1..672
                     /locus_tag="VFF72_10055"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HZV93551.1"
                     /translation="KVTALLEEFRIVKRQLLLAALAARRAGGGHAAQRVLVCSPLPGE
                     GKTFCATNLALALAAEKDSEVVLVDADFAKPSVLSTLGLTGRHGLMDALADPSIRVED
                     CVIPTDVGGLYVLPAGNPTTSDSEYLASARTPEVLDRLTQGAPNRMVIFDSPPALAAT
                     PAAELAKYVSQALVVARADLTGQSALEDALSLLSACPDIKLLLNAAHFSPSGRRFGTY
                     YGYGE"
     gene            669..>2154
                     /locus_tag="VFF72_10060"
     CDS             669..>2154
                     /locus_tag="VFF72_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93552.1"
                     /translation="MTLAARLSGWLVCGLAALALPGAARADDTDAKGLHAGPHTEIAP
                     YIEADQVVTAEIDPGSDVVTYSTLAAGADATIVGHYSAASASVRYERHFSESRGLADS
                     DTVSGIARASYAIVPRAVTIEAGALASREGVDGSGGTFTGALVGDSGVASTIYSGYIG
                     PSVKTQAGDLQIEGHYRFGYTKVQEPDAVVVGTTGAIPVDLTDESTTHNAQAHIGFRP
                     YTIAPVGVGVGGQWVEQDVSNLDQRVRDRNVRGDVIVPLNPSFALAGGAGYEDVEVSS
                     RDALRDGSGAPVIGKDGRYVTDDSAPRRIAYQTDGLIWDVGVMWRPSTRTSLEAYVGR
                     RYDSMTYYGTFAYAPNSRQSVNVSVYDGISSFGGSLTDEIAGLPTEFTAVRNVLSGSI
                     GGCVASLEGGNCVGSTFGSLASAAFRSRGFTASYSARFGRTTAGLGFGYDHRQFIAAP
                     GTVMAAANGVVDQTWWGAVYLTSKLDERSGVNFSATETLFDSGFA"
BASE COUNT          319 a          764 c          743 g          328 t
ORIGIN      
        1 aaggtcaccg cgctgctgga ggaattccgc atcgtgaagc gccagttgtt gctggcggcg
       61 ctcgccgcac ggcgcgccgg gggcggccat gcggcgcagc gcgtgctggt ctgctcgccg
      121 ttgccgggcg agggcaagac cttctgcgcc accaacctag ccctcgcgct cgccgccgag
      181 aaggacagcg aagtcgtgct ggtcgatgcc gatttcgcca agccttcggt cctctcgacg
      241 ctcggcctga ccggccggca cggcctgatg gacgcgctgg cggacccgtc gatccgcgtc
      301 gaggactgcg tgatcccgac cgatgtcggt gggctgtacg tgcttcccgc gggcaatccg
      361 acgacctcgg acagcgaata cctcgccagc gcccgcacgc cggaagtgct cgaccgcctg
      421 acgcaaggcg cgccgaaccg catggtgatc ttcgattcgc ccccggcgct ggcggcgacg
      481 ccggcggcgg aactggcgaa atacgtcagc caggcgctgg tcgtcgcccg ggcggacctg
      541 accgggcaat cggcgctgga agacgcgctg tcgctgctct cggcctgccc ggacatcaag
      601 ctgctgctca acgcggcgca tttcagcccc agcggccgcc gcttcggcac ttattacgga
      661 tacggcgagt gaccctcgcg gcgcgccttt ccgggtggct ggtctgcggc ctggcggcgc
      721 tggcgctgcc gggcgcggcg cgggccgacg acaccgacgc caaaggcctg cacgccggcc
      781 cgcacaccga gatcgccccg tacatcgagg ccgatcaggt ggtgaccgcc gaaatcgatc
      841 ccggcagcga cgtggtcacc tattcgaccc tggccgcagg ggccgacgcc accatcgtcg
      901 ggcattattc ggccgcttct gcctcggttc gctacgagcg ccatttcagc gagagccgcg
      961 gcctcgccga cagcgacacg gtcagcggta tcgcccgcgc gtcgtacgcg atcgtgcccc
     1021 gcgcggtgac gatcgaggcc ggcgcgctcg cctcgcgcga aggggtggac ggcagcgggg
     1081 gcaccttcac cggcgcgctg gtgggcgaca gcggcgtggc gagcacgatc tactccggtt
     1141 acatcgggcc ctcggtcaaa acgcaagcgg gcgacttgca gatcgaaggc cattaccgtt
     1201 tcggctacac caaggtgcag gagccggacg cggtggtggt cggcaccacc ggcgccattc
     1261 cggtcgatct caccgacgag agcacgaccc ataacgccca ggcccatatc ggcttccgcc
     1321 cgtacacgat cgccccggtg ggcgtcggcg tgggcggcca atgggtcgag caggacgtct
     1381 ccaacctcga ccagcgggtg cgcgaccgca atgtccgcgg cgacgtgatc gtgccgctca
     1441 atccgtcgtt cgcgctcgcc ggcggggccg gctacgagga tgtcgaagtg tcgagccgcg
     1501 acgccctgcg cgacgggtcg ggcgccccgg tgatcggcaa ggacggccgc tacgtcaccg
     1561 acgacagcgc cccgcgccgg atcgcctacc agaccgacgg gctgatctgg gatgtcgggg
     1621 tcatgtggcg cccgagcacg cgcacctcgc tcgaagccta tgtcggccgc cgctacgatt
     1681 cgatgactta ttacggcacc ttcgcgtatg cgccgaactc gcgccagagc gtgaacgtgt
     1741 ccgtctatga cggcatcagc agcttcggcg ggtcgctgac cgacgagatc gctggcctgc
     1801 cgaccgagtt caccgcggtg cgcaatgtgc tgtcaggcag catcggcggg tgcgtcgcga
     1861 gcctcgaggg cggcaactgc gtcggcagca cgttcggctc gctggcgtcg gccgccttcc
     1921 gcagccgcgg gttcacggcg tcctattcgg cgcgcttcgg gcgcaccacc gccgggctcg
     1981 gcttcggcta cgaccatcgc cagttcatcg ccgcgccggg cacggtgatg gccgcggcca
     2041 acggcgtggt cgaccagacc tggtggggcg cggtctatct caccagcaag ctcgacgagc
     2101 gttccggcgt caacttcagc gccaccgaga cactgttcga cagcggcttt gccg
//
LOCUS       DASRWS010000534         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3094570, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000534 DASRWS010000000
VERSION     DASRWS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094570"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2137)
                     /locus_tag="VFF72_10065"
     CDS             complement(<1..2137)
                     /locus_tag="VFF72_10065"
                     /inference="COORDINATES: protein motif:HMM:NF025240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HZV93553.1"
                     /translation="MPEGILFVTYPTLRSGRSDVTRLGQLLEWAGDGFDGVIVFDEAH
                     AMANAAGGEGSRGRTKGSEQGVAGVRLQNLLPRARVLYASATGASDVNNLAYAGRLGL
                     WGPETAFADRETFVAGIRDGGIAAMELVARDLKSLGLYTARALSFAGVEYEILEHRLT
                     AEQIAVYDAYAEAWAIIHANLRDALEATRIVDAESGETLNRGAKSAALSIFEGTKQRF
                     FAQLLLSMKLPSLLPAIAAARDEGQAVVVQLVSTAEAMLERRLAELSDDEREALEIDL
                     SPREYVVDYLAKSFPVRLMAVFTDENGNPRSEPMMDDEGRPVLCRSALAARDRMIERL
                     CALPPIGTALDAIIERFGVDQVAEVTGRSRRLIVGPDGRQKLQARSARANIAETQAFM
                     DGAKRILVFSDAGGTGRSYHADLSAGNQARRVHFLLEPGWRADAAIQGLGRTNRTNQA
                     SAPLFRPVTTDVRGERRFISTIARRLDSLGALTRGQRQTGGQNLFDPADNLESQYAKE
                     ALNRWFGLLFVGKLDAVGLGRFQELTGLRVEGADGTMTDDLPTIQRWLNRILALPIAL
                     QNAIFDEFLALVEARVDAAQQAGTLDLGVETIAVENFEVQSDTLLRADPLSGATTHLL
                     ELEIARALKPLTLSRLEEHYGIDSDHHQPLRNARSNRVGLLLPARSLLTDEGARIARF
                     ELLRPLKREYITADQLAESSWEPLEPAEFR"
BASE COUNT          362 a          682 c          726 g          379 t
ORIGIN      
        1 gccggaattc agccggttcg agcggctccc agctgctctc ggccagctgg tcggcggtga
       61 tatattcccg cttgagcggg cgcagcagtt cgaaccgcgc gatccgcgcg ccctcatcgg
      121 tcaacagact gcgcgccgga agcaatagcc cgacccggtt cgagcgcgca ttgcgcagcg
      181 gctgatggtg atcgctgtcg atcccgtagt gctcctctag cctcgacagg gtcagcggct
      241 tcagtgcccg ggcaatttcc agctcgagga gatgcgtggt ggcgccggat aacggatcgg
      301 cacgtagcag ggtgtcggac tgcacctcga agttttcgac cgcaatcgtc tcgacgccta
      361 ggtcgagcgt cccggcctgt tgggcggcat cgacgcgggc ctcgacgagg gcaagaaact
      421 cgtcgaaaat cgcgttctgc agcgcaatcg gcaaggcgag gatgcgattg agccatcgct
      481 gaatcgtcgg caagtcgtcc gtcatggtgc cgtcggcgcc ctctacgcga agacccgtaa
      541 gctcctgaaa ccggcccaga ccgacggcgt cgagcttgcc cacgaagagc aggccaaacc
      601 agcggttgag cgcctccttg gcgtactggc tttccagatt gtcggcggga tcgaacaggt
      661 tctgtccgcc ggtctggcgt tgaccccggg tcagggctcc gaggctgtcg agacgccgcg
      721 cgattgtcga gatgaagcgt cgttcgcccc gcacatcggt cgtcaccggc cggaatagcg
      781 gcgccgaggc ctggttcgtc cgattggtcc ggccgaggcc ctggatcgcg gcatcggccc
      841 gccagcctgg ttcgagcagg aagtggactc ggcgagcctg gttcccggcg gagagatcgg
      901 catggtagct gcgtccggtg ccgccggcgt cggaaaagac caggatgcgc ttggcgccat
      961 ccatgaatgc ctgggtctcg gcgatattgg cgcgggcgct gcgcgcctgc agcttctggc
     1021 gtccatcggg accgacgatg agccgccggc tgcgtccggt tacttcggcc acctggtcga
     1081 cgccgaaacg ctcgatgatc gcatcgagcg cggtgccgat cggtggcagg gcgcacagcc
     1141 gttcgatcat tcggtctcgc gccgcaagag cggaccggca aagaaccggt ctgccttcat
     1201 cgtccatcat cggctccgac cgcggattgc cgttctcgtc ggtaaacacg gccatcaggc
     1261 ggaccgggaa actcttggca aggtagtcga cgacgtattc gcgcggagag agatcgattt
     1321 cgagcgcttc gcgttcgtcg tcggacagtt cggcgaggcg tcgctcgagc atggcctcgg
     1381 cggtcgagac cagctgaacg acgacggcct ggccttcatc gcgtgctgcc gcaatcgccg
     1441 ggaggaggct ggggagcttc atcgacagca ggagctgggc gaagaagcgt tgctttgtgc
     1501 cctcgaatat cgagagggcg gccgatttcg cgccgcggtt gagcgtctcg ccgctctcgg
     1561 catcgacgat ccgggtggct tcgagcgcgt cgcgcaggtt tgcgtggatg atcgcccacg
     1621 cttcggcgta agcgtcgtaa actgcaatct gctcggccgt cagacgatgc tcgaggatct
     1681 cgtattcgac gccggcgaag gagagcgcgc gggccgtata gagaccgagc gacttgagat
     1741 cgcgagcgac gagctccatc gccgcgatgc cgccgtcgcg aatgcccgcg acgaacgttt
     1801 cacgatccgc gaaggccgtt tcgggtcccc acaggccgag acgcccggcg taggcgagat
     1861 tgttgacgtc ggaagctccc gtggcagagg cgtagaggac tcgcgcccgc ggcagcagat
     1921 tttgcaggcg gacacccgcg acgccttgct cggagccttt cgtccgcccg cgcgagcctt
     1981 cgccgcccgc agcgttggcc atcgcatgcg cttcatcgaa aacaatcacc ccgtcgaagc
     2041 cgtcgccagc ccattcgaga agctgtccga gccgcgtgac atcgcttctc ccggagcgca
     2101 gcgtcggata agtgacgaac aggatgcctt ccggcattgt gatcggccg
//
LOCUS       DASRWS010000535         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1086776, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000535 DASRWS010000000
VERSION     DASRWS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086776"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFF72_10070"
     CDS             complement(<1..473)
                     /locus_tag="VFF72_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93554.1"
                     /translation="MRYLVVGAGALGGYFGGRLLEAGCDVTFLLRPARAAAISARGLI
                     VRSRLGDMTLSPPHILASDIRAPFDVVIVACKAYDLESTMDDFAPAVGATTAILPLLN
                     GMRHLDALQARFGAQRVLGGLCLISAVVDADGVVQHVNDLHSLTFGEIAGGLSPR"
     gene            complement(775..1485)
                     /locus_tag="VFF72_10075"
     CDS             complement(775..1485)
                     /locus_tag="VFF72_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZV93555.1"
                     /translation="MRLQPFLDISQSDDLETFERRLVDFSHEFEFGLVNAILVLDRPG
                     RDAQFVSVGNTPEGYADAFSSVESSRRDPVLKRLKRMSVPFTYDQDLYINESAGDVWE
                     EQAAFGYRTGVAVALHLQDHKHFLLGVDREKPLPGDVDEVTRLLASLQLLAVHAQDAA
                     LRLMGVQDAAKEVPRLTQRELEILRWTMEGKSAWAVGQILSVSESTVNFHLRNVFRKL
                     GSSSKHQAVLKAMSLGLL"
     gene            1588..1971
                     /locus_tag="VFF72_10080"
     CDS             1588..1971
                     /locus_tag="VFF72_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93556.1"
                     /translation="MIAAWQIRSMSDVKVDVATWRESMASLCHLGTTSRRGDDGRRCG
                     QAVWGLHIDGAGAIGIAWDWEEVRAGVVAMSDPMTVSSNASFIDDSGSPVDDFKRILH
                     LNNAIFSLPWQEAVRQGEADLRAAA"
     gene            2247..>2594
                     /locus_tag="VFF72_10085"
     CDS             2247..>2594
                     /locus_tag="VFF72_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZV93557.1"
                     /translation="MTHSIGPEGDVFVVGAGIMGIGIAQVAAQAGHRVRLFDAREGAA
                     AAACAQLARTLDGLVAKGRIDASEAQAISARVQAVSALSDAAGSGLVVEAIVENADAK
                     RALFTSLDAIAKPG"
BASE COUNT          427 a          836 c          901 g          430 t
ORIGIN      
        1 atgcgcggcg acaggccgcc tgcgatctcg ccgaaggtca gcgagtgcag atcgttgacg
       61 tgctggacga cgccgtcggc gtcgacgacc gccgagatga ggcacaggcc gcccaggacg
      121 cgctgcgcac cgaagcgggc ctgcagcgcg tcgaggtggc gcatgccgtt gagaagcggc
      181 aggatggccg tcgtggcgcc gacggccggg gcgaagtcgt ccatcgtcga ctcgaggtcg
      241 taggccttgc aggcgacgat gacgacgtcg aacggggcgc ggatgtcgct ggccagaatg
      301 tgcggcggcg acagcgtcat gtcgcccagc cggctgcgaa cgatcagccc cctggccgag
      361 attgccgcgg cacgagccgg tcgaagcagg aaagtgacgt cgcagcctgc ctcgagcagg
      421 cggcctccga aatatccacc cagcgcgccg gcgccgacaa cgaggtatcg catggtggtg
      481 cgagcctact ctgccttgga aaacggcgtc gcgccgaagg acgggaaggc aatcaccggg
      541 caaacgacga aagccggacc tcggtccggc tttgatgggt ttttgagctg gggttctgga
      601 cttaccaggt gcccccgcgc ggaatcccga agctcaggcg gatcgctttc gacatgctgt
      661 tctcctcatc ccacgcgggc cgcgcagtct gaagctgatt tcagcgaaga accgcgcgcg
      721 tcgaaaccta caaacctttg taggcgtcgt ggcgcgaaga cacgcgcgcc gcggctacag
      781 cagtccgagc gacatcgcct tcaggacggc ctggtgcttg gacgacgacc cgagcttgcg
      841 aaagacgttg cggagatgga aattgacggt gctctcgctg accgaaagga tctggcccac
      901 ggcccaggcc gacttacctt ccatggtcca gcgcaggatc tcgagctcgc gctgcgtcag
      961 gcgcggcacc tccttcgccg cgtcctgcac gcccatgagc cgcagcgccg catcctgggc
     1021 gtgaaccgcg agcagctgca gcgaagcgag gaggcgcgtc acttcgtcga cgtcgccggg
     1081 tagcggcttc tcgcggtcga cgccgagaag gaagtgcttg tggtcctgca ggtgcagcgc
     1141 caccgcgact ccggtgcggt agccgaacgc tgcctgctcc tcccagacgt cgcccgcgct
     1201 ctcgttgatg tagagatcct ggtcgtaagt gaaggggacg ctcatccgtt tcagccgttt
     1261 gagcaccggg tcgcgtctcg agctctcgac gctcgagaag gcatccgcgt agccctccgg
     1321 cgtgttgccg acgctcacga actgggcatc gcgcccgggt cgatcgagca cgaggatggc
     1381 attgaccagg ccgaactcga attcatggct gaagtcgacc aggcggcgtt cgaacgtctc
     1441 gaggtcgtcg ctctggctga tgtcgagaaa cggctgaagc ctcatttgtt cacgatatcg
     1501 gtcggttccc tacaagccct gacgggttgt cgaagtcggc tcgcgtgccg aacactgcaa
     1561 ccgtcaacac ttttcgagga gatttcgatg atcgcagcct ggcaaatccg gtcgatgagc
     1621 gatgtcaagg tcgatgtcgc gacgtggcgc gaatccatgg cctcactatg ccacctcggg
     1681 acgacgtcca ggcgtggcga cgatgggcgg cgttgcggcc aggccgtctg ggggctgcac
     1741 atcgacggtg ccggcgccat cggcatcgcc tgggactggg aagaggtgcg cgccggcgtc
     1801 gtcgcgatgt cggatccgat gacggtgtcg tcgaacgcat cgttcatcga cgacagcggc
     1861 tctccggtcg acgatttcaa gcgcatcctg cacctgaaca acgcgatctt ctcgctgccg
     1921 tggcaggagg ccgtgcggca aggcgaagcc gaccttcgcg ccgctgcctg aaggccggag
     1981 atcgccggcg attccggcga cccttttccg atcgccgtcg agcgcactct ggcgatctgc
     2041 ggccgggacc tgcggacccg tggacccgtg gacccgcggt cctcggcctc gcggactggg
     2101 cggctcgagt cggcgacggg agttcttgcg ccttcgcgct ttgcggcgtg ccgcgcggcg
     2161 gcggagacgc gcggcggccc tggcgccgcg acggacggcc gcactgtcga taatcgccgg
     2221 ctcgcgttcc atcaccaggc tgcacgatga cccactccat tggtcccgag ggcgacgttt
     2281 tcgtggtcgg ggcgggcatc atggggatcg gcattgccca ggtcgccgca caggcggggc
     2341 accgagtgcg gctgttcgac gcgcgggaag gtgccgcggc ggcggcctgt gcgcaactcg
     2401 ccaggaccct cgacggcctg gtcgcgaagg gccggatcga cgcctccgag gcgcaggcga
     2461 tctcggctcg cgtccaggcg gtttccgcct tgtccgatgc cgcgggcagc gggcttgtcg
     2521 tcgaagcgat cgtcgagaac gccgacgcca agcgggcgtt gtttacgagc ctcgatgcga
     2581 ttgccaagcc cggc
//
LOCUS       DASRWS010000536         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_224290, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000536 DASRWS010000000
VERSION     DASRWS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224290"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFF72_10090"
     CDS             complement(<1..540)
                     /locus_tag="VFF72_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZV93558.1"
                     /translation="MTVKNRIPADSPWAQRRLLWLEREVSLTRWMHSASTHGWLVRVL
                     SALSRIGDGWLWYASVLLLPLFGGPSGPSAAVRMFAVGAIDIVLYRIVKRWIARPRPA
                     RACEGIRECARALDEFSFPSGHTMHAVACSIILSSYYPALALFVWPFTVLVALSRVIL
                     GLHYPSDVIVGAAIGAVTAL"
     gene            complement(563..832)
                     /locus_tag="VFF72_10095"
     CDS             complement(563..832)
                     /locus_tag="VFF72_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93559.1"
                     /translation="MKKPPSADDFNHTAWAIRCVLIESSGTVFLAIDESSDLAPLPCA
                     GSDPCFAARREATPATQPMPNVRAEMWFRKTARPAACKPAIHERA"
     gene            918..2684
                     /locus_tag="VFF72_10100"
     CDS             918..2684
                     /locus_tag="VFF72_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZV93560.1"
                     /translation="MKSHLDAIAIARVVLTDGLAILSAAVATWFAAVSAARCTRIRAR
                     RKEGMSSFCSRLLGPVGNPSMIAWLGLSGLLSLAGACWARAVLLMIAVALGGCAVLPS
                     QIERPASYARLDVSDTTLARIAAASTPADARELSGFRLLPDGAQAFEARLALIRRAER
                     TIDVQYYLIASDRTGLEFLRELELAAARGVRVRILVDDLYATGEDELFSEVARHERIE
                     VRMFNPLPARTGGIVERIALSLHEFSRINRRMHNKLLIADGTYAVTGGRNIADAYFDR
                     SGDANFIDVDLLAAGPVVARLAAVFDRYWNSRLAYPLGSLIGARRAVPARQSPAAAAL
                     AAVATTAPSTLDAELASGRLVLMRASADVIADAPEKADGDNAAPTVAQAHLALLGSAA
                     SRVLLASPYFVPGSAAMQTLAGMRTRDVEISVLTNSFATTDEPLVHLGYARHRDALLG
                     AGVDLHELMASSEPTGEATTSLGGSGHGRSLGRLHTKLTVIDDEQTFLGSMNMDARSA
                     DLNTEAAIVVHSAALARLAAAFVRTWQRDRSYELRLRDEKLTWLSGKGASQRSRSVEP
                     RPVATPALPVRVLATLLGESVL"
     gene            complement(2756..2845)
                     /locus_tag="VFF72_10105"
     tRNA            complement(2756..2845)
                     /locus_tag="VFF72_10105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2809..2811),aa:Ser,seq:tga)
     gene            complement(2910..>3351)
                     /locus_tag="VFF72_10110"
     CDS             complement(2910..>3351)
                     /locus_tag="VFF72_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HZV93561.1"
                     /translation="QTLELAGALETRLREAPLREFLDSMFGNEPTLWSDSLVGDDRLR
                     FTLNTLTRIRFVDSDGRLDFAAKDGAGTAPPGLVPWFDAAGRRSAGTPIAFGHWSTLG
                     LLQRGDLLGIDTGCVWGGKLTAVRVDGGRRDVCQVDCEQSQRPG"
BASE COUNT          523 a         1195 c         1131 g          502 t
ORIGIN      
        1 cagggcggtg acggcgccga tggccgcgcc cacgatcacg tcgctcggat agtgaaggcc
       61 gaggatgacc cgcgacaggg cgacgagcac ggtgaacggc cagacgaaga gggcaagcgc
      121 cgggtagtag ctcgacaaaa tgatgctgca ggccaccgca tgcatcgtgt ggccgctcgg
      181 aaagctgaat tcatcgaggg ctcgcgcgca ctcgcggatg ccttcgcagg cgcgtgccgg
      241 ccggggccgg gcgatccagc gcttgacgat gcgatagaga acgatgtcga tcgctcccac
      301 cgcgaacatc cgcaccgccg ccgacggtcc cgacggaccg ccgaagaggg gaagcagcaa
      361 cacgctcgcg taccagagcc agccgtcgcc gatccggctc aacgcggaga ggacgcggac
      421 cagccagccg tgcgtcgagg ccgagtgcat ccatcgcgtc agcgagacct cgcgctcgag
      481 ccagaggagc cgccgctgcg cccagggcga gtcggcggga attcgattct tgactgtcat
      541 gacgtgcgca ccttagcaaa cgtcatgccc tctcgtggat tgcgggcttg caggcagccg
      601 ggcgagcggt cttcctgaac cacatttcgg cgcggacgtt cggcatcggc tgggttgccg
      661 gcgtcgcctc tcgcctcgca gcaaagcacg ggtcggaacc ggcgcacggc agcggcgcga
      721 gatcgctcga ctcgtcgatg gccaggaaga ccgtcccgct ggattcgatc agcacgcatc
      781 gaattgccca ggctgtgtga ttgaagtcat ctgcactggg cggcttcttc actggaagcg
      841 cggcggtcgg tttccgagac tgcttccaca ccgaagcgac aggtcgcaac ggtgagcgaa
      901 cgaacgagga gcctgccgtg aagagccatc tagacgccat cgccatcgcc cgggtcgttc
      961 tcaccgacgg cctcgccatc ctgtcggccg cggtcgcgac ctggttcgcg gccgtctcag
     1021 cagcacgttg cacacgcatc cgcgcccgcc ggaaagaagg catgtcttcc ttctgtagcc
     1081 gcttgctggg tccggtcggc aacccttcga tgattgcctg gctcggtctg tccggcctgc
     1141 tcagcctggc cggcgcgtgc tgggcgcgcg ccgtcctgct catgatcgca gtggcgctcg
     1201 gcggctgcgc cgtgctgccg agccagatcg agcgacccgc ctcgtatgca cggctcgacg
     1261 tctccgacac gacgctggcc cggatcgcag cggcttcgac gccggccgac gcacgcgagc
     1321 tgtccggctt tcgcctgttg cccgacggcg cgcaggcctt cgaggcccgc ctggccctga
     1381 ttcgccgggc cgagcgaacg atcgacgtcc agtactacct gatcgccagc gaccgcaccg
     1441 ggctcgagtt cctgcgcgag ctcgagctgg ccgccgcccg cggcgtgcgc gtacgcatcc
     1501 tcgtcgacga cctctacgcc accggcgagg acgagctgtt ctccgaggta gcccggcacg
     1561 aacgcatcga agtccggatg ttcaacccgc tgccggcacg aaccggtggc atcgtcgagc
     1621 gcatcgcgct gtcgctgcac gagttctcgc gcatcaaccg ccgcatgcac aacaagctcc
     1681 tcatcgccga cggcacctac gccgtgaccg gcggcaggaa catcgccgac gcctacttcg
     1741 accgcagtgg cgacgccaac ttcatcgacg tggatctcct ggccgccggc ccggtggtgg
     1801 cgcggctggc ggcggtcttc gatcgctact ggaactcgcg gctggcctat ccgctgggca
     1861 gcctgatcgg cgcgcggcgg gcggtgccgg cgaggcagtc gccggccgct gccgcgctgg
     1921 ccgcggttgc gacgaccgcg ccgtccacgc tggacgccga gctcgccagc ggccgactcg
     1981 tcctgatgcg cgccagcgca gacgtgatcg ccgacgcgcc ggaaaaggcc gatggcgaca
     2041 acgccgcgcc gactgtcgcg caagcccacc tcgcgttgct cggatcggcg gcgtcacggg
     2101 tgctgctggc gtcgccctac ttcgttccgg gcagcgcggc gatgcagacc ttggccggca
     2161 tgcgcacgcg cgacgtcgag atctcggtcc tgaccaactc cttcgcgacc accgacgagc
     2221 cgctcgtgca cctcggctac gcccggcacc gcgacgcgct cctgggcgcc ggcgtcgatc
     2281 tgcacgagct catggccagc agcgagccga caggcgaagc gacgacctcg ctcggcggtt
     2341 ccggacacgg tcgctcgctc ggccggctgc ataccaagct caccgtgatc gacgacgagc
     2401 agaccttcct cggctcgatg aacatggacg cgcgctcggc cgatctcaac accgaagccg
     2461 cgatcgtcgt ccacagcgcc gccctggccc gcctcgccgc cgccttcgtg cgcacctggc
     2521 aacgcgaccg cagctacgag ctgcggctgc gggacgagaa gctgacctgg ctgtcgggga
     2581 agggcgcgag ccagcgcagc cgctcggtcg agccgcggcc cgtcgcgacg cccgcgctgc
     2641 cggtccgcgt gctggcgacg ctgctcggtg aaagcgtgct ttgagcggaa gatggctcta
     2701 cgcgtgcgcg gcttcggcgg agccgtcgag acatgaacgc atctgtcgcg cgcactggcg
     2761 gagacggtga gattcgaact cacgaagggg tctccccctc ggcagttttc aagactgctg
     2821 ccttcaaccg ctcggccacg tctcctgtgc acggccgaag ccgttcgcct ggcacgcatt
     2881 ctggcatcgg cgcgctactg cgccaatgat cacccgggtc gctgcgattg ttcgcagtcg
     2941 acctggcaga cgtcgcgccg gccaccgtcg acgcgtaccg cggtgagctt gccgccccag
     3001 acgcagccgg tgtcgatgcc cagcaggtcg ccgcgttgca ggaggccgag cgtcgaccag
     3061 tggccgaacg cgatcggcgt tccggcgctg cgtcgccccg cggcgtcgaa ccacggaacg
     3121 agtccgggcg gcgccgtccc cgcgccgtcc ttggccgcga aatcgagccg gccatcgctg
     3181 tcgacgaagc ggatccgggt cagcgtgttg agggtgaagc gcagccggtc gtcgccgacg
     3241 agcgaatcgc tccagagggt cggctcgttg ccgaacatcg aatccaggaa ctcacgcagc
     3301 ggcgcctcgc gcaggcgcgt ctcgagcgcg ccggcgagct cgagcgtctg c
//
LOCUS       DASRWS010000537         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_801570, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000537 DASRWS010000000
VERSION     DASRWS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801570"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="pyrC"
                     /locus_tag="VFF72_10115"
     CDS             <1..822
                     /gene="pyrC"
                     /locus_tag="VFF72_10115"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZV93562.1"
                     /translation="PLMTLYLTDSTPPALMRSAREAGIVALKLYPAGATTNSDAGVTD
                     LRKIAGVLEAAEREGIALLVHGEVTDPEIDVFDREAVFIDRHLAALRRDFPALRIVLE
                     HVTTREGVQFVAGAGERTGATLTAHHLLYNRNALFSGGVRPHYYCLPILKREEHRRAL
                     IDAATSGPGRFFLGTDSAPHASRLKEHASGCAGCYTAFNALELYAEAFDAAGALDRLE
                     AFASFNGADFYGLPRNDGKVTLRREASVVPEALAFGDATLKPLRAGERISWTFAS"
     gene            complement(897..1334)
                     /locus_tag="VFF72_10120"
     CDS             complement(897..1334)
                     /locus_tag="VFF72_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93563.1"
                     /translation="MPLPILAKRIEEISMKTKIALSAIVAAVAVFSQGAFAQASSPTR
                     AEVKAEAKTAAKAPAGEAGAGELKRPSTHSTKTRAQRKAETKAALKAHEGEAMGEAGS
                     MKSQNTAGSTTTRAERKAKTKAALKAHEGTPMGQADSNAEPKK"
     gene            1571..1940
                     /gene="rnpB"
                     /locus_tag="VFF72_10125"
     ncRNA           1571..1940
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFF72_10125"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1995..3230
                     /locus_tag="VFF72_10130"
     CDS             1995..3230
                     /locus_tag="VFF72_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZV93564.1"
                     /translation="MNPDARTLWQAPTWALAVLLDCLSMLGPFSIDTYLPAFTGISRA
                     IGATPVEMQQTLSAYLFGFAVMNLFHGAMSDSFGRRPVILAGLVVFTVASVGCALSQH
                     VGVLVLFRALQGMSAGAGVVISRAVIRDMFPQADAQRVMAQVTIYFGVAPAVAPMVGG
                     WLFIHTGWHSIFWLLAAIGIALFVAIARLLPETLHVTHRQPFNAAHLLRGYWSLGSNP
                     RFLTLALASGIPFNGMFLYVLSTPVFLGESLHLGPGEFFWFFLLTIGGIVAGAWCSGR
                     MAGKVRPSRQIRLGFLIMGVVSIVNLVLNLLFVPEAWWALLPIAVYSFGWAMMVPVVT
                     LLVLDLVPERRGMASSFQSFVGSTANGVVAGVISPLVMHSTVALAATSLGMMSIGVFA
                     WLWVRPRLGLPPRRSPLPV"
     gene            3355..4119
                     /locus_tag="VFF72_10135"
     CDS             3355..4119
                     /locus_tag="VFF72_10135"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZV93565.1"
                     /translation="MNPSSPSQEAGARRELAACYNLFDTLGWAEGIFNHITVRVPSPA
                     GAGPHYLINPFGIHYAEVTAGNLVKIDVDGRDVDDSGRDVNRAGFVIHSAIHAARDDA
                     HCVMHVHTTAGCAVSCKESGLRHDNFYSAMMSGDVGYHDYEGVTTSVEERPRLVRSLA
                     GHSHLILRNHGLLVVGATLPQAFQRLWTLQRACEIQLASDAGAGPNRAIAADVLERVP
                     ATRLRMATTGDDVATRLFDAMVRRAGIAFSRGAENP"
     gene            4138..5127
                     /locus_tag="VFF72_10140"
     CDS             4138..5127
                     /locus_tag="VFF72_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZV93566.1"
                     /translation="MTQADSSLPGFEAIRLGGNIGAEIRGLDLRRPLPAATFEALNAA
                     FVRHEVLVFRDQDITLDEQMAFARQFGELSIHPFSPNLDDKREVIVLDYSADNPPALT
                     DQWHADETFRAAPPAITVLRANIVPAYGGDTLFASMTAAYTGLSERMKQYIHGLEAIH
                     DFKPWRPLFTSSQAHQVKLRELERAFPNPSHPVVRIHPLTGRRVINVNAQFTTRIKGL
                     GADESQMILQYLYDRAKVPEFQLRVKWQPHTVVMWDNRSVHHYAPHDYYPQRRSMNRV
                     TVAGDAVRGVSGPYVAEEGVEALPDGHHVTPAAGTRRPTREFERGLVVAPEAT"
     gene            5124..>5851
                     /locus_tag="VFF72_10145"
     CDS             5124..>5851
                     /locus_tag="VFF72_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV93567.1"
                     /translation="MNAPLRRHVLAALGGGLCAAALRPAWAQSTFPDRPIKLTVPYPP
                     GALTDALGRMVAERLRVGFGQPVIVENKAGAGTLLGAASVAKSAPDGYQLLIATSTTL
                     AISPAMFSSPAAVPGDFAGVAMIGSVSLLLVTRPDLKVASLPELVALMRREPGKYSYG
                     SPGNGTMHQLLVEMIKVQEKVQATHIPYQGSMTALRDLLTGRIDFMFLDVVAALPQVK
                     AGKINAIAVSAAHRLPALPDVPTVA"
BASE COUNT          871 a         2059 c         1944 g          977 t
ORIGIN      
        1 ccgctgatga cgctctacct caccgattcg acgccgcccg cgctgatgcg cagcgcccgc
       61 gaggcgggca tcgtcgcgct caagctctac ccggccggcg cgacgacgaa cagcgacgcc
      121 ggcgtcaccg acctgcgcaa gatcgccggc gtgctcgagg cggccgagcg cgagggcatc
      181 gcgttgctcg tgcacggcga ggtgaccgat ccggagatcg acgtcttcga tcgcgaagcc
      241 gtcttcatcg acaggcatct ggccgccctg cggcgcgact ttccggccct gcgcatcgtc
      301 ctcgaacacg tcacgacgcg ggagggcgtg cagttcgtcg ccggggccgg agagcggacc
      361 ggcgcgacgc tgacggcgca ccatcttctc tacaaccgca acgcgctttt cagcggcggc
      421 gtgaggccgc actattactg cctgccgatc ctcaagcgcg aggagcatcg gcgcgcgctg
      481 atcgacgcgg ccacctcggg gcccggccgg ttcttcctcg gcaccgacag cgcaccgcac
      541 gcgtcccggc tcaaggagca cgccagcggc tgcgccggct gctacacggc gttcaacgcg
      601 ctcgagctgt atgccgaggc cttcgatgcc gcgggcgcgc tcgatcggct cgaagcgttc
      661 gccagcttca acggggccga cttctacggc ctgccccgaa acgacggcaa ggtgacattg
      721 cggcgcgagg catcggtggt gccggaagcg cttgcgttcg gcgacgccac gctcaaacct
      781 cttcgcgcgg gcgaaaggat ctcttggacg ttcgcttcct agccgtcgcg cagcgaaccg
      841 caggcgcaaa aaaaggcggc ccgggggccg cctttctcgt cgcccgaaag cgagtcttac
      901 ttcttcggct cggcgttcga gtcggcctgg cccatcggcg ttccctcgtg ggccttcagg
      961 gcggccttgg tcttggcctt gcgctcggcg cgggtggtcg tggagccagc ggtgttctgg
     1021 ctcttcatcg aaccggcttc tcccattgcc tcgccctcgt gggccttcag cgcggccttg
     1081 gtttcggcct tgcgctgggc gcgggtcttg gtggaatggg tcgacggcct cttcagttcg
     1141 ccggcaccgg cttcgccggc gggcgccttg gcggcggtct tcgcctcggc cttgacttcg
     1201 gcgcgggtcg gcgacgaggc ctgggcgaac gcgccctgcg agaacacggc gacggccgcg
     1261 acgatggcac tcagagcgat tttggtcttc atggaaatct cctcaattcg tttggcaagg
     1321 atgggaaggg gcatcaagca agtctcgact ctcggttcgc gatccctgcc gggttcaacg
     1381 ggttgcacga cgaggagcag gacttgacac gacaacgcgg caccggcgga ctcggttgac
     1441 gcatcgccga gcggtgctct tggccccgca accggtcatt ttactcaata cggcgaaatc
     1501 cgcggctcgg ggcagcgccg cgcggcgagg atgtcgcgtg attgaaacgt cggccgataa
     1561 tcccgggggt gaagcgagcc aggccgccgc tcgcgctgtc ctcgggcggc gcgggaggaa
     1621 ggtccggact gcacagggcg gcgtagcagc taacggctgt ccaccgcgag gtgaggatca
     1681 gagcaacaga gacgagtcgt tgccgggcgc cgagaggcac cggcggcggg tgaaacgggc
     1741 aatctctacg cgcagcaata ccaaataggc cggcgtgcgc ggggcttcgg caccgcgtgg
     1801 aagtgtggct ccgcacgagc cggcgggtag gtagcaccga gccgggcagg gatgcccggc
     1861 ccagaggaat ggcggccacg gcggggacgt cgcgagacga cctcgccgca cagaatccgg
     1921 cctatcggct cgcttcacac taactcgatc tccggcgtgc gcggcgacgc tgactacact
     1981 cgaccgcttc ctcgatgaac cccgacgcga gaaccctgtg gcaggcgccg acctgggcgc
     2041 tggccgtgct gctggactgc ctcagcatgc tcgggccgtt ctcgatcgac acctatctgc
     2101 cggccttcac agggatctcg cgcgcaatcg gtgcaacgcc ggtcgagatg cagcagacgc
     2161 tttccgcgta cctgttcggc ttcgcggtga tgaacctgtt ccacggcgcg atgtccgaca
     2221 gcttcggccg ccggccggtc atcctggccg gtctggtggt cttcaccgtc gcctcggttg
     2281 gttgcgcgct gtcgcagcac gtcggcgtgc tcgtgctctt tcgtgcgctg caggggatgt
     2341 cggccggggc cggagtggtc atctcgcgcg ccgtcattcg cgacatgttc ccgcaggccg
     2401 acgcgcagcg cgtgatggcc caggtgacca tctacttcgg cgtcgccccc gcggtggcgc
     2461 ccatggtcgg cggatggctg ttcatccata cgggctggca ttcgatcttc tggctcctgg
     2521 ccgccatcgg catcgcgctg ttcgtggcga tcgccaggtt gcttccggag acgctgcacg
     2581 tgacgcaccg gcagccattc aacgccgccc acctgctgcg cggctactgg tcgctcggca
     2641 gcaacccccg cttcctgacc ctggcactgg ccagcgggat tcccttcaac ggcatgttcc
     2701 tgtacgtcct gtcgaccccg gtgttcctcg gcgagagcct ccacctgggg cccggcgagt
     2761 tcttctggtt cttcctgctc accatcggcg gcatcgtcgc cggcgcctgg tgctcggggc
     2821 gaatggccgg caaggtcagg ccgtcacgcc agatccggct cggtttcctg atcatgggcg
     2881 tcgtctcgat cgtcaacctg gtgctcaacc tgctcttcgt gcccgaggcg tggtgggcgc
     2941 tgctgccgat cgcggtctat tcgttcggct gggcgatgat ggttcccgtc gtcaccctgc
     3001 tcgtcctcga tctcgtcccc gagcgccgcg gcatggcgtc gtcgttccag tccttcgtcg
     3061 gcagcacggc caacggcgtc gtcgccggcg tcatctcgcc gctggtgatg cactcgacgg
     3121 tcgccctcgc cgcgacctcg ctcgggatga tgagcatcgg cgtgttcgcg tggctctggg
     3181 tccgacccag gctcggcctg ccgccgcggc gctcgccgct gcccgtatga gcggccatga
     3241 cctcaccggt tgcgacaagc cggtccgtgg cttggcgaaa ggaaggcggc gcgcggtaaa
     3301 acacggacga cgatttcttt cgaatattgc cggccttggc ggcaagattg cccgatgaac
     3361 ccttcaagtc cttcccagga agcgggcgcg cgccgggagc tcgcggcctg ctacaacttg
     3421 ttcgacacgc tcggctgggc cgagggaatc ttcaaccaca tcaccgtgcg ggtgccgtcg
     3481 ccggccggtg ccggcccgca ctacctcatc aacccgttcg ggatccacta tgccgaggtc
     3541 acggccggca acctcgtgaa gatcgacgtc gacggccgcg acgtcgacga ttcggggcgc
     3601 gacgtcaacc gggccggctt cgtcatccac agcgccatcc acgcggcgcg cgacgatgcc
     3661 cattgcgtca tgcacgtgca cacgaccgcc ggctgcgcgg tctcgtgcaa ggagagcggc
     3721 cttcgacacg acaacttcta cagcgccatg atgtccggcg acgtcggcta tcacgactac
     3781 gaaggcgtga cgacgagcgt cgaggagcgt ccgcgactgg tgcgttccct cgccggccac
     3841 agccacctca tcctgcgcaa ccacgggctg ctggtcgtcg gcgcgacgct gccgcaggcg
     3901 ttccagcgcc tgtggacgct gcagcgagcc tgcgagatcc agctcgccag cgacgccggc
     3961 gcgggcccga accgcgccat cgccgccgac gtgctcgagc gcgtgccggc gacgcggctg
     4021 cgcatggcga cgaccggtga cgacgtcgcg acgcgcctct tcgacgcgat ggtccgtcga
     4081 gccggcatcg ccttctcgcg tggcgccgaa aatccctgac accgaggaga cgatcccatg
     4141 acccaagccg attcctccct cccgggcttc gaggcgatcc gcctcggcgg caacatcggc
     4201 gccgagatcc gcggcctcga cctgcgccgt ccgcttcccg cggcgacgtt cgaggcgctg
     4261 aacgccgcgt tcgtccgcca cgaggtgctc gtctttcgcg accaggacat cacgctcgac
     4321 gaacagatgg ccttcgcccg ccagttcggc gaactgtcga tccatccgtt cagccccaac
     4381 ctcgacgaca agcgcgaggt gatcgtcctc gactactcgg ccgacaatcc gccggcgctg
     4441 accgatcagt ggcatgccga cgagaccttt cgcgcagccc cgccggcaat cacggttctg
     4501 cgtgccaaca tcgtcccggc ctacggcggc gacacgctgt tcgccagcat gacggcggcc
     4561 tacaccggcc tgtcggagcg catgaagcag tacatccacg gactcgaggc gatccacgac
     4621 ttcaagccgt ggcgcccgct cttcaccagc tcgcaggcgc accaggtcaa gctgcgcgag
     4681 ctcgagcggg cgtttccgaa cccgtcgcat ccggtggtgc gcatccatcc cctgacgggt
     4741 cgccgggtga tcaacgtcaa cgcccagttc acgaccagga tcaagggcct cggcgccgac
     4801 gagtcgcaga tgatcctgca gtacctctac gaccgggcca aggtgcccga attccagctg
     4861 cgcgtgaaat ggcagccgca cacggtcgtc atgtgggaca accgctcggt gcaccactac
     4921 gcgccgcacg actactaccc gcagcggcgc agcatgaacc gcgtcaccgt cgccggagac
     4981 gcggttcgtg gcgtcagcgg accctacgtc gcggaagagg gcgtcgaggc actgccagac
     5041 ggtcatcacg tcacgcccgc cgccggcacg cggcggccga cgcgcgaatt cgaacgcggc
     5101 ctcgtcgttg cgccggaggc gacatgaacg cgccgttgcg tcgacacgtt ctcgcggccc
     5161 tgggcggcgg cctctgtgcg gcggcgctgc ggccggcgtg ggcgcagtcg acctttcccg
     5221 atcgacccat caagctgacc gtgccgtacc cgccgggcgc gctcaccgac gcgctcggca
     5281 ggatggtcgc ggagcggctg cgcgtcggct tcggtcaacc ggtcatcgtc gagaacaagg
     5341 ccggcgccgg cacgctgctc ggcgccgcat cggtggcgaa atcggcgccc gacggctacc
     5401 agttgctcat cgcgacgagc acgacgctgg cgatctcgcc ggcgatgttc tcgtcgccgg
     5461 cggccgtgcc gggcgatttc gccggcgtcg cgatgatcgg cagcgtcagc ctgctgctcg
     5521 tcacccggcc ggatctcaag gtggcgagcc tgccggagct cgtggcgttg atgcgccgcg
     5581 agcccggcaa gtactcgtac ggctcgcccg gcaacggcac catgcaccag cttctcgtcg
     5641 agatgatcaa ggtgcaggag aaggtgcagg cgacgcacat tccctaccag ggcagcatga
     5701 cggcgcttcg cgatctcctg accggccgga tcgacttcat gttcctcgac gtcgtcgcgg
     5761 cgctgccgca ggtgaaggcc ggcaagatca acgcgatcgc cgtgtcggcc gcgcaccggc
     5821 tgccggcgct gcccgacgtg ccgaccgtgg c
//
LOCUS       DASRWS010000538         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2802334, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000538 DASRWS010000000
VERSION     DASRWS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2802334"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFF72_10150"
     CDS             <1..614
                     /locus_tag="VFF72_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93568.1"
                     /translation="AELLQEPMDEADRARFVANIAGETERIQELVDRMMELTALESRK
                     SLDQAEPVAVVALVADVVAAASPAAQARGLRLEFDAPQGESPVVEGDAFLLRRAIANL
                     VDNALDFSPPGGRVRLAVKLHRRSCDIVVGDEGPGIPEYAEDKVFEKFYSLARPATAR
                     KSTGLGLSFVKEIAELHHGRAGLKNGVRGAVATLSLPLVAASA"
     gene            complement(627..1493)
                     /locus_tag="VFF72_10155"
     CDS             complement(627..1493)
                     /locus_tag="VFF72_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZV93569.1"
                     /translation="MNDSSPAFRLHGPAEPRTPLVLDSPHSGFEFPDDFGARLGEFEL
                     REGEDCFVDELWMPATERGVGLIAARAPRTYIDCNRHAGDIDLALLEDGHWPDPHVPS
                     GKARLGKALIWRTLDDGRDIYARKLSVDEVRRRIERFHRPYHQALRQRIEMTRARFGA
                     SWHIDCHSMNSVAGAQGEGGAGTPRADIVLGDRDGTTCDADFTEFIRAHLAARGYEVR
                     INDPYKGVELVRAYSDPADRRMSLQLEVNKRLYMDEARCEKTAGFATLQADLASLVDA
                     LLERAKDELRSR"
     gene            1610..1789
                     /locus_tag="VFF72_10160"
     CDS             1610..1789
                     /locus_tag="VFF72_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93570.1"
                     /translation="MRLFRVPLTPQSEIGRVVFAVIVSVVATALLLEWAPSPSPGTMH
                     GKSATVANAKEAGSP"
     gene            complement(2025..>2691)
                     /gene="phnE"
                     /locus_tag="VFF72_10165"
     CDS             complement(2025..>2691)
                     /gene="phnE"
                     /locus_tag="VFF72_10165"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZV93571.1"
                     /translation="IDAPTQMADLLTRMWPIDFEEFRRGAGQAMLETINIASIGTLIT
                     LVMAIPIGIMAARNITRIPALNWFAQFLLVASRSVNSLVWALLFVAVFGPGALAGTIA
                     IAFRSIGFVGKLFAESLEESQRGPIEALRAVGAPPLSVLLKGYWPQVKPAFWSLVLLR
                     WDINVRESAVLGLVGAGGIGVALQTAMDFFQWNRVALILLLVFTVVIFAELAVNLIRR
                     RLI"
BASE COUNT          455 a          895 c          926 g          415 t
ORIGIN      
        1 ccgccgagct gctgcaggag ccgatggacg aggccgaccg ggcgcgattc gtcgccaaca
       61 tcgccggcga gaccgagcgc atccaggaac tcgtcgaccg gatgatggag ctgacggcgc
      121 tcgagtcgag aaagagcctc gaccaggccg agccggtcgc cgtcgtcgct ctggtcgccg
      181 acgtcgttgc cgccgcgagc ccggccgcgc aggcgcgggg ccttcgcctc gaattcgacg
      241 cgccgcaggg cgaatctccg gtcgtcgagg gcgacgcttt cctgctgcga cgggccatcg
      301 ccaacctcgt cgacaacgcg ctcgatttct cgccaccggg aggccgggtg cgcctggccg
      361 tgaagctgca tcgacgcagc tgcgacatcg tcgtcggcga cgaggggccc ggcattcccg
      421 aatacgcgga agacaaggtg ttcgagaagt tctattcgct ggcccggccg gcgacggcca
      481 ggaagagcac aggcctcggc ctgtcgttcg tcaaggagat cgccgagctg caccacgggc
      541 gcgccgggct gaagaacggc gttcgaggtg ctgtcgcgac gctctcgctg ccgctcgtcg
      601 ccgcgtcggc ctgacacgcc gacccgctat ctggaccgca attcgtcctt cgcgcgctcg
      661 agcagtgcgt cgacgagcga agcgaggtcg gcttgcagcg tcgcgaaccc ggcggtcttc
      721 tcgcaccgcg cctcgtccat gtagagccgc ttgttgacct cgagctgcag gctcattcgc
      781 cggtcggccg ggtcggaata ggcccgcacc agctcgacgc ccttgtaggg atcgttgatg
      841 cgcacttcgt agccgcgcgc cgcgagatgc gcgcgaatga actcggtgaa gtcggcgtcg
      901 caggtcgtac cgtcgcgatc gccgaggacg atgtcggcgc gcggcgttcc ggcgccgcct
      961 tcgccctggg cgccggcgac cgagttcatc gaatggcagt cgatgtgcca gctggcgccg
     1021 aagcgcgcgc gcgtcatctc gatgcgctgg cgcagcgcct ggtgataggg gcggtggaat
     1081 cgctcgatgc gtcgacgcac ctcgtccacg gaaagcttgc gcgcatagat gtcgcggccg
     1141 tcgtcgaggg tgcgccagat gagcgccttg cccagccgcg ccttgccgct cggcacgtgc
     1201 ggatcgggcc aatggccgtc ttcgagcagc gccagatcga tgtcgccggc gtgccggttg
     1261 cagtcgatgt aggtgcgcgg cgcccgcgcc gcgatcaggc cgacgccgcg ctcggtggcc
     1321 ggcatccaaa gctcgtcgac gaagcaatcc tcgccttcgc gcaattcgaa ttcgccgagg
     1381 cgggcgccga aatcgtcggg gaactcgaac cccgaatgcg gcgaatcgag gactagcggc
     1441 gtccgcggct cggccgggcc gtggagccgg aatgccggag aggaatcgtt catcgccaca
     1501 gcatagcccg cttcgcgctc gcttcatcga cgcttcgaaa tggcttcgcc gcctccggtc
     1561 aggcttgggc cacggtcggc ttgcggaccc tgtttccaag gagagaacga tgcgactgtt
     1621 ccgggttccc ctgacgccgc aaagcgagat cggccgcgtc gtcttcgcgg tgatcgtctc
     1681 ggtggtcgcg accgcgcttc tgctcgaatg ggcgccatcg ccatcgccgg gcacgatgca
     1741 tggcaagagc gcgaccgtcg ccaatgcgaa ggaggccggt tcgccctgac cggtcggagg
     1801 acacgagcgc caaaggcccg ctcgcgcccg cggctggtgc cggccttggc cgcagggcga
     1861 agtcggtcat gccgtgatat ggatgtctcg tgcttttccc gaaaatggcg aacgcatgtc
     1921 ggattggaag ggccatcccc ttcccgttcc gtcctgcgaa aaggcgagaa gccggtcgaa
     1981 gcctcgcagg cataggagct gcaagcaagg ctgctagcgc gccgtcaaat cagccgccgg
     2041 cggatgaggt tgaccgccaa ctcggcgaag atgacgacgg tgaagaccag caacaggatc
     2101 agcgcgacac gattccactg gaagaagtcc attgcggtct gaagggcgac gccgatgcct
     2161 ccggcgccga cgagccccag caccgcggat tcgcgcacgt tgatgtccca gcgcagcagc
     2221 accagcgacc agaacgccgg cttcacctgc ggccaatagc ccttgaggag aacgctgagc
     2281 ggtggcgcgc ccaccgcacg cagtgcctcg atcggaccgc gctgcgactc ctcgagcgac
     2341 tccgcgaaga gcttgccgac aaagccgatc gagcggaatg cgatcgcgat cgtgccggcg
     2401 agcgctccgg ggccgaatac cgcgacgaag agcaacgccc agaccagcga attgacggag
     2461 cgggatgcga ccagcaggaa ctgcgcgaac cagttgagcg ccggaatgcg ggtgatgttg
     2521 cgcgccgcca tgatcccgat cgggatcgcc atcacgagcg tgatgagcgt gccgatcgac
     2581 gcgatgttga tggtctcgag catcgcctgg ccggcgccgc gacggaactc ctcgaagtcg
     2641 atcggccaca tccgcgtcag caggtcggcc atctgggtcg gcgcgtcgat g
//
LOCUS       DASRWS010000539         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3957981, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000539 DASRWS010000000
VERSION     DASRWS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957981"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2037
                     /locus_tag="VFF72_10170"
     CDS             <1..>2037
                     /locus_tag="VFF72_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZV93572.1"
                     /translation="LERPSSGPSLTARLKDNAHHLLRAYETTVTAAGNGEAITPAAEW
                     LLDNYHLVEKQVRQIRDDLPASYYRQLPKLGAGSFAGYPRVFGLAWAFIAHTDSHFDS
                     DLLCRFVQAYQTVQPLTIGELWAVAITLRIVLIENLRRLADEITDGRIARLDADAMAD
                     RLLGVSGHAPQAIETLLVRYGRSVSHDTFAVQLIQRLHDQDQEAMPAIAWLHARLIAQ
                     NTTADRIVAAVHQNQSAASLTVRNVITSMRLISDVDWSDLFESMSLVDECLRVSPGFA
                     DMDFTTRNLYRGEIEMLSRGSSTSEIDIANRAVAAAQAAAQGTAADESGAARAMRRHA
                     ADPGYYLLGAGRHAFETEVGYRPPATLWRHCFTRRAGLGGYIIRILLLAALLLAGVLA
                     LLAGFVPQPGWLALLGLAGFLPALDAAVAFANRGVMRDVNAVVLPGLALRDGVPAALR
                     TVVAVPTLLTTQAALAEQIERLEIHHLASGAGEIYYALLSDWTDSPTEQADGDQSLLA
                     AAATRIAELNRRYPATQDSARFLLLHRRRIWNRKQKQWMGWERKRGKLHELNRLLRGA
                     SDTSFVAIDGHAPTVPPQVRYVITLDADTRLPRDTVRRLIGKMAHPLNQPVFDAASQR
                     VVEGYGVLQPRVTPSLPVGAEGSLFQRILSSPSGIDPYAAAVSDVYQDLFDEGSY"
BASE COUNT          323 a          658 c          680 g          376 t
ORIGIN      
        1 ctggagcggc cttcctccgg cccctcgctg acggcgcggc tcaaggacaa cgcgcaccat
       61 ctcttgcggg cctatgaaac gacggtgacg gcggcgggaa atggcgaggc gatcaccccg
      121 gccgcggagt ggctgctcga caactaccat ctggttgaaa agcaggtccg ccagattcgc
      181 gacgacttgc ctgccagcta ctaccggcaa ttgcccaagc tgggcgccgg ctcgttcgcc
      241 ggctacccgc gcgtgttcgg ccttgcgtgg gcgttcattg cgcataccga cagtcatttc
      301 gactccgact tgctgtgccg gttcgtgcag gcctaccaga ccgtgcagcc gctgaccatc
      361 ggcgaacttt gggcagtggc gattacgctg cgcatcgtgc tgatcgagaa ccttcggcgc
      421 ctggccgacg aaatcaccga cggccgcatt gcgcggctgg acgccgatgc gatggccgat
      481 cgcctgctcg gcgtgagcgg ccacgcgccg caggccatcg aaaccctgct ggtgcgctac
      541 ggcagatcgg tttcgcacga tacctttgca gtgcagttga tccagcggct gcacgaccag
      601 gaccaggagg cgatgccggc cattgcatgg ctgcacgcac gtctgatcgc ccagaacacc
      661 acggcggacc ggatcgtggc cgcggtgcac cagaatcaga gcgcggccag cctgacggtg
      721 cgcaacgtga ttacgagcat gcgtctgatt tccgatgtgg actggagcga tctgttcgaa
      781 agcatgagtc tggtcgacga gtgtttgcgc gtttccccgg gattcgccga tatggatttc
      841 accacgcgca acttgtatcg cggcgaaatc gaaatgctgt cgcgcggttc atcgacgagc
      901 gagatagaca tcgccaaccg cgccgtcgct gccgcgcagg ctgccgcgca aggcacggcg
      961 gccgacgagt ccggcgctgc gcgagcgatg cgccggcatg ccgccgatcc cggttattac
     1021 ttgctggggg cgggccggca tgctttcgag accgaggtcg gctatcgccc gccggcgacg
     1081 ctatggcggc actgcttcac gcgccgcgcc ggtctgggcg gctacatcat ccgcatcctg
     1141 ttgctcgcgg cgctgctgtt ggccggcgtc ctggcgctgc tggccggctt tgtgccccag
     1201 ccaggctggc tggccttgct gggcctggcg ggattcctgc ccgcgctgga cgcggccgtg
     1261 gcgtttgcca atcggggtgt aatgcgcgac gtcaacgcgg tcgttctgcc gggactggcg
     1321 ctgcgcgatg gcgttcccgc cgcgctgcgc acggtcgtgg ccgtgccgac tctgctgacc
     1381 acccaggctg cgctggccga acagatcgag cggctggaga ttcatcatct ggccagtggg
     1441 gcgggcgaga tctactacgc attgctgtcg gactggacgg attccccgac cgagcaggcc
     1501 gatggcgacc aatccctgct ggccgccgcg gccacgagga tcgcggaatt gaaccggcgc
     1561 tatccggcta cgcaggacag cgcgcgcttc ctgctgctgc atcgccggcg tatatggaat
     1621 cgcaagcaaa agcagtggat gggttgggag cgcaagcgcg gcaagctgca cgagttgaat
     1681 cgtctgctgc gcggcgcgtc ggacacttcg ttcgttgcca ttgacggaca tgcgccgacc
     1741 gtgccgccgc aagtgcgcta tgtaatcacg ctcgatgcgg acactcgttt gccgcgcgat
     1801 acggttcgcc ggctgatcgg aaagatggcg catccgctca accaaccggt gttcgatgcg
     1861 gcgagccagc gcgtcgtcga aggttatggc gtgctgcagc ctcgtgtcac gccctcattg
     1921 cctgtcggcg ccgagggctc gctgtttcag cgcatcttgt cgagcccgag cgggatcgat
     1981 ccctacgccg cggccgtatc cgacgtgtat caggatctgt tcgacgaagg ctcgtac
//
LOCUS       DASRWS010000540         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2490625, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000540 DASRWS010000000
VERSION     DASRWS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490625"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFF72_10175"
     CDS             <1..414
                     /locus_tag="VFF72_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV93573.1"
                     /translation="AATRYFNTFASSPLQAAVGMAVLDVIERDHLADNVAKVGAFLKA
                     ALSQRKGRFGSIADVRGHGLFIGVEIAKADAARTPDADGAIEVVNRLKDKGFMTSNAG
                     AYRNVLKIRPPLTFGQKHAEDFLVAFDETMAELDG"
     gene            407..1411
                     /locus_tag="VFF72_10180"
     CDS             407..1411
                     /locus_tag="VFF72_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZV93574.1"
                     /translation="MDKEAIGRLFAPAARAALPAFPIEPREITLVSLAENVTFKVTDR
                     DNAYVLRLHRPGYHTLDELVSERAWIRALADAGIDVPGPVPARDGRDYVPVTIPATGE
                     QRFAGMLRWTEGRLLSHVLAETSDPRVVEDYFAQLGALTAAMHNQASAWRPPSGFSRH
                     ALDRDGLMGEAPHWGPFWEHRSLSPAERRLLLEARAHMREKLGRLSRDPSVYSLIHAD
                     MHPGNILVDGDRLTVIDFDDAGFGWHAYDIAIVLTYYQSKPNAREIERAFLDGYRATR
                     PLTDETLALIPMFRLTRWMASIGWFHQRPELEPSPVFEERKAWVLEKCAALRIGDRWT
                     "
     gene            complement(1632..>2345)
                     /locus_tag="VFF72_10185"
     CDS             complement(1632..>2345)
                     /locus_tag="VFF72_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HZV93575.1"
                     /translation="PVDMAWDVLRHYRDVADRLPDELSLVGTLAHAPDGSGQKLAAIV
                     LCHCGSLAEGDKAVQPIKTFGKPVMDAIAPIPYTQMNAMLDGGYPRGALNYWKSSFLA
                     TLSDDAIRAMIDCFARCPTTMGQLLLEHFHGAVTRIGPTDTAFPHRSPGHNLLVLAQW
                     TDRRQNAECIAWARDSYDSMQAFMGAGRYVNYLGDDEARDAISTAYGPNYRRLQQIKA
                     TYDPANFFRMNQNIQPLAS"
BASE COUNT          375 a          817 c          767 g          386 t
ORIGIN      
        1 gcggcgacgc gctacttcaa caccttcgcg tcgagcccgt tgcaggccgc ggtcggcatg
       61 gccgtgctcg acgtcatcga gcgcgatcac ctggccgaca atgtcgccaa agtcggcgcg
      121 ttcctgaagg cggcgctcag ccaacgcaag ggccgcttcg gctcgatcgc cgacgtgcgc
      181 ggccatggcc tgttcatcgg cgtcgagatc gcgaaggccg acgcggcccg cacgcccgat
      241 gccgacggcg cgatcgaggt ggtcaatcgc ctgaaggaca agggcttcat gaccagcaat
      301 gccggcgcct accgcaacgt gctgaagatc cggccaccgc ttacctttgg gcagaagcac
      361 gccgaggact tcctggtcgc gttcgacgaa acgatggccg agctcgatgg ataaggaggc
      421 catcggccgc ctcttcgcgc cggccgcccg cgccgccctc cccgccttcc cgatcgagcc
      481 gcgcgagatc acgctggtct cgctcgccga gaacgtcacg ttcaaggtga ccgatcgcga
      541 taacgcctac gtgctgcgct tgcaccggcc gggttatcac acgctcgatg aactggtgtc
      601 cgagcgggcg tggatccgcg cactggccga cgccggcatc gacgtgccgg gtccggtgcc
      661 ggcgcgcgac ggacgcgact atgtcccggt gacgatcccc gccaccggcg agcagcgctt
      721 cgccggcatg ctgcgctgga ccgaaggccg cctgctgtcg cacgtcctcg ccgagaccag
      781 cgatccccgg gtggtcgagg actacttcgc ccaactgggc gcactgaccg cggcgatgca
      841 caaccaggcg agcgcctggc ggccgccctc gggcttctcc cgccatgcgc tcgatcgcga
      901 cgggttgatg ggcgaggcgc cgcattgggg gccgttctgg gagcaccgct cgttgtcgcc
      961 cgccgagcgc cgcctgctgc tcgaagcccg ggcacacatg cgcgagaagc tcggacgtct
     1021 cagccgcgac ccgtcggtgt acagcctgat ccatgccgac atgcatcccg gcaacatcct
     1081 ggtcgacggc gaccggctga ccgtcatcga cttcgacgat gccggcttcg gctggcacgc
     1141 ctacgacatc gccatcgtgc tcacctacta tcagagcaag ccgaacgccc gcgagatcga
     1201 gcgcgccttc ctcgacggct accgcgccac ccggccgctc accgacgaga cgctggccct
     1261 gatcccgatg tttcggctga cccgctggat ggcgtcgatc ggctggttcc accagcggcc
     1321 cgagctcgaa ccctcgccgg tgttcgagga gcgcaaggcc tgggtgctcg agaaatgcgc
     1381 cgcgctccgc ataggagacc gatggacctg aggaacgcag cgctcgccgc cggagctggc
     1441 ccgcgtcgtc ggcaagacgc gcccggctcg atctcgcgtc cgccttcgag cgcctgcatc
     1501 agctccgaag cagaaggcag aacgctacag gccagtcccg gtgcgcattc gctccgggta
     1561 tcggcgtagc cgccacccag ccaatgcttc ccggtcctac cggctcccgg tcggctcggg
     1621 ccgctgccgg gttacgaagc gagtggctgg atgttctgat tcatgcggaa gaagttcgca
     1681 ggatcgtacg tcgccttgat ctgctgcaga cgtctgtagt tcggcccata ggccgtcgag
     1741 atggcgtcgc gagcctcgtc gtcaccgagg tagttgacgt atctgcccgc ccccatgaac
     1801 gcctgcatac tgtcatagct atcgcgtgcc caggcgatgc actcggcgtt ctgccgtcgg
     1861 tccgtccatt gcgccaacac cagcagattg tggccgggtg accgatgggg gaaggcagtg
     1921 tcggtggggc cgatgcgcgt gacggcgccg tggaagtgtt cgagcagcaa ctggcccatc
     1981 gtcgtcgggc agcgcgcgaa gcaatcgatc atggcacgga tggcatcgtc gctcagcgtc
     2041 gcgaggaagc tcgacttcca gtagttgagc gcgccgcgcg gatagccccc gtccagcatg
     2101 gcgttcatct gcgtgtacgg gattggagcg atggcatcca tcaccggctt accgaacgtc
     2161 ttgatcggct gcaccgcctt gtcgccttcg gccagcgatc cgcaatggca aagcacgatt
     2221 gccgccagtt tttgccccga gccgtccggc gcgtgcgcga gcgtgccgac caggctgagc
     2281 tcgtcgggaa gccggtccgc cacgtcgcgg tagtggcgca ggacatccca tgccatgtcg
     2341 acggg
//
LOCUS       DASRWS010000541         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1088044, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000541 DASRWS010000000
VERSION     DASRWS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088044"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..631
                     /locus_tag="VFF72_10190"
     CDS             359..631
                     /locus_tag="VFF72_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZV93576.1"
                     /translation="MNKTELIEHIARQADISKAAASRALEAMIGGVKTSLKKNHTVSL
                     VGFGTFSVSKRAARSGRNPRTNEPIKIKAAKVPKFRPGKALKDALN"
     gene            662..737
                     /locus_tag="VFF72_10195"
     tRNA            662..737
                     /locus_tag="VFF72_10195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Val,seq:tac)
     gene            857..2779
                     /locus_tag="VFF72_10200"
     CDS             857..2779
                     /locus_tag="VFF72_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZV93577.1"
                     /translation="MFDFFRRHTRVLQFVLVLLVFPSFVFFGIQGYSRFSGNDQKTVA
                     TVAGQPISQDEFDNAMRDRIDRARRQMPNLDAKLFDTPQMRQLALDAMIRERVLLAAA
                     DKLHLAPGDERLDKLFKSDPQFAQFRNPDGSLNKDMVAALGLSSEGFAAQLRQDMARQ
                     QVLQGLNDSVLAPEAATAAALDAMYQQREVQVERFDTKSYLDKVSPTDAQIEAYYKDP
                     ANAAQFRAPEQESIDYVVLDPEAMKKGLTISDADLKKYYDENVKRYTTPEERKASHIL
                     VKADKDAPKAERDKARAKAEALLAEVRKDPASFAEIAKKNSDDEGSASKGGELDWFGK
                     GAMVKPFEDAAFALKPGEISGIVPSDFGFHIIRLDAIRGGQVRPFAEVRPEIESEIRN
                     QLAQKRFSDAAVEFGDTVYEQPDSLKPAADKWQLEIKKAEHVTRQPAPGASGPLANAR
                     FLEALFSSDATKSKHNTKAIDIGSNQLVSGHVTQYTPAHQLPLADVKDRIRTQLATMQ
                     AAALAKKAGMERLEAARAAPAKPVSSNTLIVSRAQSHDLQASVVDAVLKAPAAKLPAF
                     VGVDLGSAGYDVVKITKLWGRDPGFADLDKARSQYGQVWSDAVDRAYYDELKSRFKVK
                     INEAALASSGGSTEDR"
     gene            2829..2904
                     /locus_tag="VFF72_10205"
     tRNA            2829..2904
                     /locus_tag="VFF72_10205"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2862..2864,aa:His,seq:gtg)
     gene            complement(2984..3961)
                     /locus_tag="VFF72_10210"
     CDS             complement(2984..3961)
                     /locus_tag="VFF72_10210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZV93578.1"
                     /translation="MHAWLCENPTGVDALQWKDLPTPQPGPGEVRVAIRAASLNFPDL
                     LIVQNKYQVKPALPFVPGSEYAGTIEAVGEGVRHLHVGDPVAAFGGTGGFATHACVNA
                     DLVVPLPEGFAFDDAAAFILTYGTTHHALLDRAALKAGETLLVLGAAGGVGTAAIQLG
                     KVVGARVIAAASSAEKCELCRSIGADATIDYSSGNIRDELKKLTDAKGPDVVYDPVGG
                     DLAEPVFRSIAWRGRYLVIGFAQGGIPALPLNLALLKGASLVGVFWGEFARREPKANA
                     RALAELARWYAEGKIKPVIDQRLPMRELPAAYARMGSRKVRGKLVMTNT"
     gene            4064..4819
                     /gene="surE"
                     /locus_tag="VFF72_10215"
     CDS             4064..4819
                     /gene="surE"
                     /locus_tag="VFF72_10215"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041929570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZV93579.1"
                     /translation="MRILIANDDGYLAPGLEALVRACEGLGDLDVVAPEQNASGTSNS
                     LTLNRGLSVYEARNGYRYINGTPADCVHFALSGLLDQRPDLVVSGINQGANMGDDTLY
                     SGTVAAAMEGFLLGIPAIAFSQVQKGWEHLDAATRVARAVIEQVLEDPPAQSIYLLNV
                     NIPNRADADRLPRRVTRLGRRHASEPVIRQVSPRGDTLYWIGPAGDAREAGPGTDFHA
                     TRHGEVSITPLHVDLTDHATLPSWRQWADKAPR"
     gene            4816..>5560
                     /locus_tag="VFF72_10220"
     CDS             4816..>5560
                     /locus_tag="VFF72_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZV93580.1"
                     /translation="MTAEGRPRSRFPLRLDRLPAVGSPGGTVLRPQRDLAAAYPDAGR
                     SGSPAGLGLDSIDVRRRMVERLRDGGVGQSQVIEALLRVPRHSFVDTALATQAYEDTS
                     LPIGHGQTISKPSVVARMLEMLFEGTNARRHGNLGAVLEIGTGCGYQAALLSELSVRV
                     ISMERLKPLHEKARDLLAPIRSNRLRLVHGDGMLGHPPNAPYDSIIAAAGGAQVPEAW
                     LDQLAVGGRLVSPVDQADGSQALVVVDRRE"
BASE COUNT          992 a         1897 c         1774 g          897 t
ORIGIN      
        1 gtcgcgtctc gtcggcttcc tgtacttctc gaagcctgaa ccctgatcgc ctgctgctgc
       61 caacgtgatc tgcttcatcg ccgccctccg tgtccgacgc tgacacatca acgcatgcag
      121 ctacgatcca gagtacttga tcagcgaatc cctagcgccc gcgtcgcgga agtcaagtgc
      181 gaaccgcacg agcggcgaat gttttttgcg tctccggcgt cgcaatagaa tcgccaaccc
      241 tgcttgacac tggccgatcg cgacgatgta atcgtttcgc ccaggccgca gaccgccgcc
      301 tcgcgccgcc gcacccaatt cctttgagac accgagcctt cctgaggggg aaccattcgt
      361 gaacaagact gaactcatcg agcacatcgc caggcaggcc gacatctcga aagcggcggc
      421 atcgcgcgcg ctcgaggcga tgatcggcgg cgtgaagacg agcctgaaga agaaccacac
      481 cgtctcgctc gtcggcttcg gaacgttcag cgtcagcaag cgcgcggcgc gcagcggtcg
      541 caatccgcgc accaacgagc cgatcaagat caaggcggcc aaggtaccga aattccgtcc
      601 gggcaaggcg ctcaaggacg cactcaactg accgtcggct ccggtcggcc gacagcccac
      661 cgggtgctta gctcagccgg tagagcggcg cccttacaag gcgtaggtcg ggggttcgaa
      721 accctcagca cccaccatca ggcgagcgcg gagaatggtc gcgcccgccc ggttaccgga
      781 cgggcgctcg gatagaatcg ggcggccctc caacgaaagg cgaacctcgg ttcgcctttc
      841 tcgcatctgc cgaacgatgt tcgatttctt ccgccgccat acacgcgtcc tgcagttcgt
      901 cctcgtcctg ctggtgtttc cgtcgttcgt cttcttcggc atccagggtt acagccgctt
      961 ttcgggcaac gaccagaaaa cggtggcgac ggtcgccggg cagcccatca gccaggacga
     1021 attcgacaac gccatgcgcg accggatcga tcgggcgcgt cgacagatgc ccaacctcga
     1081 cgcgaagctc ttcgacacgc cgcagatgcg ccagctcgcg ctcgacgcga tgatccgcga
     1141 gcgtgtcctt ctcgccgccg ccgacaagct gcacctcgct ccgggcgacg agcgcctcga
     1201 caagctgttc aagtccgatc cccagttcgc ccagttccga aatccggatg gcagtctcaa
     1261 caaggacatg gtcgccgcgc tcggtctctc gtccgaaggg ttcgcggcgc aactgcgcca
     1321 ggacatggcg cgccagcagg tgctccaggg tctcaacgat tcggtgctgg cgcccgaagc
     1381 ggcgaccgcc gctgccctcg atgcgatgta ccagcagcga gaagtgcagg tggagcgctt
     1441 cgacaccaag tcctacctcg acaaggtctc gcccaccgat gcacagatcg aggcgtacta
     1501 caaggacccg gcgaacgccg cccaattccg ggcgccggag caggaatcga tcgactacgt
     1561 cgtgctcgat cccgaggcga tgaaaaaggg cctgacgatc tcggatgcgg acctgaagaa
     1621 gtattacgac gagaacgtca agcgctacac gacgccagag gagcgaaagg ccagccacat
     1681 tctcgtcaag gccgacaagg atgcgccgaa ggccgaacgc gacaaggcgc gcgccaaggc
     1741 cgaggctctg ctcgcagagg tgcgcaagga ccccgcctcg ttcgccgaga tcgcgaagaa
     1801 gaactccgac gacgaaggct cggcgtccaa gggcggcgag ctcgactggt tcggcaaggg
     1861 cgcgatggtc aagccgttcg aggatgcggc gttcgcgctg aagccgggcg agatcagcgg
     1921 catcgttccc agtgacttcg gctttcacat catccgcctc gacgcgattc gcggcggcca
     1981 ggtcaggccc ttcgccgagg ttcgtcccga gatcgagagc gagatccgca accagcttgc
     2041 gcaaaagcgc ttttccgatg cggcggtcga attcggagac accgtctacg aacaacccga
     2101 cagtctcaag cccgccgcgg acaagtggca gctcgaaatc aagaaggccg agcatgtgac
     2161 tcgccaaccc gcgcccggtg catcgggccc gctcgccaac gccaggttcc tcgaggcgct
     2221 cttctcgagc gatgcgacga agagcaagca caacacgaag gcgatcgaca tcggctccaa
     2281 ccagctcgtc tcgggtcacg tgacgcagta cacgccagcg caccagctgc cgctcgccga
     2341 cgtgaaggac aggatccgca cccagctcgc gacgatgcag gccgcagcgc tggcgaagaa
     2401 ggcgggcatg gagaggctcg aggccgcgcg cgcggcgcct gccaagcccg tgtcgtcgaa
     2461 cacgctgatc gtgtcgcggg cgcagtcgca cgatctgcag gcgagcgtcg tcgacgccgt
     2521 cttgaaggca ccggccgcga agctgcccgc gttcgtcggt gtcgatctgg gcagcgcagg
     2581 ctatgacgtg gtgaagatca ccaagctctg gggccgggat cccggattcg ccgatctcga
     2641 caaggcgcga tcgcaatacg gccaggtctg gagcgatgcg gtggaccgcg cctactacga
     2701 cgagctgaag tcgcgcttca aggtcaagat caacgaggcc gcgctcgcct cgagcggcgg
     2761 ctcgactgaa gatcgctgag gcacgaggcg tgctcaggga gccgctcgct ataattcgcg
     2821 gctcgatggt ggctgtagct cagttggtag agtccaggat tgtgattcct gttgtcgtgg
     2881 gttcgagccc catcagccac cccatctcct gagcctctgg gcggcgccgc ggcgccacgg
     2941 cgaggtcgac gcaagcgatc gctcgtgccg agaatcctct tcctcaggta ttcgtcatca
     3001 ccagcttgcc gcgcaccttg cggctgccca tgcgggcata tgcggccgga agttcgcgca
     3061 tcggcaatcg ctggtcgatc accggcttga tcttgccctc ggcgtaccag cgcgccagtt
     3121 ccgccaaggc ccgagcgttg gccttgggct cgcggcgcgc gaactcgccc caaaagacgc
     3181 cgaccagcga agctcccttc agcagcgcga gattgagcgg cagggcaggg atgccgccct
     3241 gggcgaaacc gatgaccagg tagcgaccgc gccaggcgat cgagcgaaac accggctcgg
     3301 cgagatctcc gccgaccggg tcgtagacga cgtccgggcc cttcgcgtct gtgagcttct
     3361 tcaactcgtc gcggatgttg ccgctgctgt agtcgatcgt cgcgtcggcg ccgatcgacc
     3421 ggcaaagctc gcacttttcg gcactcgatg cggctgcgat gactcttgcg ccgacgacct
     3481 tgccgagctg gatcgccgcc gtgccgacgc cgccggccgc gccgagcacg agaagcgtct
     3541 cgccggcctt cagcgcagcg cggtcgagca gggcgtggtg ggttgtgcca taggtaagga
     3601 tgaacgcggc cgcatcgtcg aaggcgaagc cctctggcag gggcacgacg aggtcggcgt
     3661 tgacgcaggc gtgtgtcgcg aagccgccgg tgccgccgaa cgcggcgacg gggtcgccca
     3721 cgtgcaggtg tcgcacgcct tcaccgaccg cctcgatcgt ccccgcgtat tcggagcccg
     3781 gcacgaaggg cagcgcgggc ttcacctggt acttgttctg gacgatgagc agatcgggga
     3841 aattgaggct cgcggcgcga atggcgaccc gcacctcgcc aggaccgggt tgcggcgtcg
     3901 gcaagtcctt ccactgcagc gcgtcgacgc cggtcgggtt ctcgcaaagc caggcgtgca
     3961 tcggtcacct cgtgtcgttc agccgcggcg agcggccctc gcggcgatga tacgcagcag
     4021 gtccgcgtca tgacaccgca tggcgactgc ctacaattcg ccgatgcgaa tcctcatcgc
     4081 caacgacgac ggctacctcg cgccgggtct cgaagcgctg gtccgggcgt gcgaaggtct
     4141 gggtgatctc gatgtcgtcg ctcccgagca gaacgccagc ggcacctcga actcgctgac
     4201 cctcaatcga ggcctctctg tctacgaggc acgcaacggc tatcgctaca tcaacggcac
     4261 gccggccgac tgcgtccatt tcgccctgtc cggattgctc gatcagcgcc ctgacctggt
     4321 cgtttcgggc atcaaccagg gcgccaacat gggcgacgac acgctgtatt cgggcaccgt
     4381 ggcagccgcc atggaggggt ttcttctcgg cattccggcg atcgccttct cgcaggttca
     4441 aaagggctgg gaacacctcg acgccgcgac gcgagtcgcg cgcgccgtca tcgaacaggt
     4501 gctcgaagat cccccggccc aaagcatcta tcttctcaac gtgaacattc ccaatcgcgc
     4561 cgatgccgac aggctgcccc ggcgcgtcac ccgcctgggt cgacgccacg ccagcgagcc
     4621 ggtgatccgg caggtcagtc cgcgtggtga cacgctctac tggatcgggc cggccggtga
     4681 cgctcgcgaa gcgggaccgg gcaccgactt ccatgccacc cgccatggcg aggtgtcgat
     4741 cacgccgctg cacgtcgacc tcaccgatca cgcgacgctg ccttcatggc gccaatgggc
     4801 cgacaaggcg ccacgatgac cgccgagggc cgcccgcggt cccgcttccc gcttcggctc
     4861 gatcgccttc cggcggtcgg ctctcccggc ggcactgtcc tgaggccgca gcgcgacctg
     4921 gcggccgcgt accccgatgc cgggcggagc ggttcgccgg ccggcctggg gctcgattcg
     4981 atcgacgtgc gccgacgcat ggtcgagcgg ctgcgtgacg gtggtgtcgg tcagtcgcag
     5041 gtcatcgagg cgttgctgcg cgtgccgcgt cattcgttcg tcgacaccgc gctggcaacg
     5101 caagcctacg aggacaccag cctgccgatc ggccacggcc agacgatttc caagccttcg
     5161 gtcgtcgcgc gaatgctcga gatgctcttc gagggcacga atgcgcgacg ccacgggaac
     5221 ctcggcgccg ttctcgagat cggtacaggt tgcggctacc aggcggcgct cctgagcgaa
     5281 ctctcggtcc gtgtcatctc catggaacgt ctcaagccgc tgcacgaaaa ggcccgcgac
     5341 ctgctcgcac cgatccgctc caatcgactt cgcctcgtcc acggcgacgg gatgctgggc
     5401 cacccgccga acgcccccta cgacagcatc atcgccgccg ccggcggcgc gcaggtgccc
     5461 gaggcgtggc tcgatcagct cgctgtcggt ggccgtctgg tttcgccggt cgatcaggcc
     5521 gatggcagcc aggctcttgt cgtcgtcgat cgccgggagg
//
LOCUS       DASRWS010000542         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2803460, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000542 DASRWS010000000
VERSION     DASRWS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803460"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="tpiA"
                     /locus_tag="VFF72_10225"
     CDS             complement(<1..635)
                     /gene="tpiA"
                     /locus_tag="VFF72_10225"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZV93581.1"
                     /translation="MPGRARKFVVGNWKMNGSRAGNAALLDGLKQALPFAADVAVCAP
                     APFIDDVSRSLAGSGIGWGAQDCSSHDAGAFTGEFSARMLAELGCSVVIVGHSERRAQ
                     HGESDGLVAAKARRAIDAGLVPIVCVGETLAERDAGRTEEVVDRQLAAVTDLLGNDIA
                     RTVLAYEPVWAIGSGRTATPDQAQAVHARLRRALGAAGASVRILYGGSVKD"
     gene            complement(644..1630)
                     /locus_tag="VFF72_10230"
     CDS             complement(644..1630)
                     /locus_tag="VFF72_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZV93582.1"
                     /translation="MKAIEITAPGGPEVLRLVDRPMPVAGTGEALIRVAASGINRPDV
                     LQRKGLYPVPPGASDIPGLEVAGKVEAGDDAALRAAGLAIGDRVCALVAGGGYAEYCV
                     APIGQCLPSPSSLSDVEAASLPETFFTVWSNVFDRGRLQPGETLLVHGGSSGIGVAAI
                     QLAKAHGARVIVTAGSKEKADACLSLGADDAIDYKTQDFVAEVRRITGKKGVDVVLDM
                     VAGDYVGRDIQCMAEDGRIVIIAVQGGVESKIDAGLVLRRRLVITGSTLRPRPVEFKS
                     AIAASLKRNVWPWLESGKVKPVVQATFAAADASRAHTLMDSHQHIGKLVLVW"
     gene            complement(1647..>3080)
                     /gene="pnp"
                     /locus_tag="VFF72_10235"
     CDS             complement(1647..>3080)
                     /gene="pnp"
                     /locus_tag="VFF72_10235"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZV93583.1"
                     /translation="KPVWDWTAPAKDDAFISKVGALAEHKLRDAYQIRSKQARTQATR
                     YVTSATLAALAEGGAAVDKVKVDNILFDIEARIVRSQILSGEPRIDGRDTRTVRPIEI
                     RTGLLPRVHGSALFTRGETQALVAATLGTDRDSQRIDALAGEYSEHFMLHYNMPPFAT
                     GETGRVGSPKRREIGHGRLAKRALVAVLPDRGEFPYSMRIVSEITESNGSSSMASVCG
                     GCLAMMDAGVPLKAHVAGIAMGLIKDGNRFAVLTDILGDEDHLGDMDFKVAGTTTGVT
                     ALQMDIKIQGITKEIMQVALAQAKEARLHILGKMQEAMGHAKEEVSQFAPRLYTMKIN
                     PEKIRDVIGKGGATIRALTEETGTTIDIGEDGTITIASTDADKAAHAKKRIEELTAEV
                     EVGRIYEGPVTKILDFGALVNLLPGKDGLLHISQIANQRVEKVTDFLKEGQIVRVKVL
                     ETDEKGRVKLSMKALLDREPREPAHHE"
BASE COUNT          440 a         1070 c         1048 g          522 t
ORIGIN      
        1 tcgtccttga cgctgccgcc gtagaggatg cgaaccgacg cgccggcggc gcccaatgcg
       61 cggcgcagcc gcgcgtggac ggcctgtgcc tggtcgggcg tggcggtgcg tccgctgccg
      121 atcgcccaga ccggctcgta ggcaaggacg gtgcgggcga tgtcgttgcc gagcaggtct
      181 gtcaccgccg ccagctgacg gtcgacgacc tcttcggtcc ggccggcgtc ccgctcggcc
      241 agcgtctcgc cgacgcagac gatcggaacg aggcccgcgt cgatggcacg acgggccttc
      301 gcggcgacga ggccgtcgct ttcgccgtgc tgcgcgcgcc gctcggagtg gccgacgatg
      361 acgacgctgc agccgagttc ggcaagcatc ctcgccgaga attcgccggt gaaggcgccg
      421 gcatcgtggc tcgagcagtc ctgcgctccc cagccgatgc cgcttccggc gagcgagcgc
      481 gagacgtcgt cgatgaaggg ggccggcgcg cagacggcca catcggcggc aaacggcaac
      541 gcttgcttca acccatccag gagcgccgcg ttgccggcgc ggctgccgtt catcttccag
      601 ttgccgacga cgaacttgcg cgcgcgaccg ggcatcgcgc cgctcaccac acgagcacca
      661 gcttgccgat gtgctggtgc gaatccatca gcgtgtgggc acgcgacgcg tcggctgcgg
      721 cgaacgtcgc ctgcacgacc ggcttcacct tgccgctctc cagccagggc cagacgtttc
      781 gcttcagcga cgcggcgatg gcactcttga actcgaccgg gcgcggacgc agcgtcgagc
      841 cggtgatgac gagtcgccga cgcaggacca ggccggcgtc gatcttcgac tcgacgccgc
      901 cctgcaccgc gatgatgacg atccggccgt cctcggccat gcactggatg tcgcggccga
      961 cgtagtcgcc cgccaccatg tcgagcacga catcgacgcc cttcttgccg gtgatccggc
     1021 gcacctcggc gacgaaatcc tgcgtcttgt agtcgatggc gtcatcggca ccgagcgaca
     1081 ggcaggcgtc ggccttttcc ttcgaacctg ccgtgacgat gacacgcgcg ccatgcgcct
     1141 tggcgagctg gatcgcggcg acaccgatgc cgctcgagcc gccatgcacc agcaatgtct
     1201 cgccgggctg caaccggccc cggtcgaaga cgttcgacca gacggtgaag aaggtctcgg
     1261 gcaggctggc cgcctcgacg tcactcaggc tggacggcga cggcaggcac tggcctatcg
     1321 gcgccacgca gtattcggcg tagccgccgc cggcaaccag cgcgcagacg cggtcgccga
     1381 tcgccaggcc ggccgcgcgc aatgccgcgt cgtcgcctgc ctccaccttc ccggccactt
     1441 cgaggccggg gatgtcggaa gcgcccgggg gcaccgggta gaggcccttt cgctgcagca
     1501 cgtccggccg gttgatgccc gaggcggcga cccggatgag cgcctctccc gtgccggcca
     1561 ccggcatcgg ccgatcgacc aggcgaagaa cctcggggcc gcccggcgcg gtgatctcga
     1621 tcgctttcac gatgtcgctc gccgcgtcac tcgtgatgcg ccggctcgcg cggctcgcga
     1681 tcgagcaggg ctttcatcga cagcttgacg cgacccttct cgtcggtctc gagcaccttg
     1741 accctgacga tctgcccttc cttcaggaag tcggtgacct tctcgacacg ctggttggcg
     1801 atctggctga tgtgaagcag gccgtccttg ccgggaagca ggttgaccag cgccccgaag
     1861 tcgaggatct tggtgaccgg accttcgtag atcctgccca cctccacttc ggcggtcaac
     1921 tcctcgatgc gcttcttcgc gtgcgccgcc ttgtcggcgt cggtcgaggc gatcgtgatg
     1981 gtgccgtcct cgccgatgtc gatcgtcgtg ccggtctcct cggtcagcgc acgaatggtg
     2041 gcgccgccct tgccgatcac gtcgcggatc ttttcggggt tgatcttcat cgtgtagagg
     2101 cgcggcgcga actgcgagac ctcttccttg gcatgaccca tcgcttcctg catcttgccc
     2161 aggatgtgca ggcgcgcttc cttggcctgc gcgagcgcca cctgcatgat ctccttggtg
     2221 atgccctgga tcttgatgtc catctgcagc gcggtcacgc cggtggtggt gccggcgacc
     2281 ttgaagtcca tgtcgccgag gtggtcctcg tcgcccagga tgtcggttag gaccgcaaag
     2341 cggttgccgt ccttgatgag gcccatggcg atgccggcga cgtgcgcctt caacggcacg
     2401 ccggcatcca tcatcgccag gcagccgccg cagaccgaag ccatcgacga cgagccgttc
     2461 gactcggtga tctcggagac gatgcgcatc gaatacggaa attcgccgcg atcgggcagg
     2521 acggcgacga gggcccgctt ggccagccgc ccgtggccga tctcgcgacg cttcggcgag
     2581 ccgacgcgcc cggtctcgcc ggtggcgaag ggcggcatgt tgtagtggag catgaagtgc
     2641 tcgctgtatt cgccagcgag cgcgtcgatg cgctgcgagt cgcgatcggt gccgagcgtc
     2701 gcggcgacca gcgcctgcgt ctcgccccgg gtgaacaacg ccgagccgtg gacgcgcggc
     2761 agcaggccgg tgcggatctc gatcggccgc acggtgcgcg tgtcgcggcc gtcgatgcgc
     2821 ggctcgcccg agaggatctg gctgcgcacg atcctggctt cgatgtcgaa caggatgttg
     2881 tcgaccttga ccttgtcgac ggcagcgccg ccctcggcga gcgccgccag cgtcgcgctc
     2941 gtgacgtagc gcgtggcctg cgtgcgggcc tgcttcgagc ggatctggta ggcgtcgcga
     3001 agcttgtgct cggcaagcgc gccgaccttc gagatgaagg cgtcgtcctt ggccggcgcc
     3061 gtccagtccc agaccggctt
//
LOCUS       DASRWS010000543         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1370335, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000543 DASRWS010000000
VERSION     DASRWS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370335"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="asd"
                     /locus_tag="VFF72_10240"
     CDS             <1..556
                     /gene="asd"
                     /locus_tag="VFF72_10240"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462931.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZV93584.1"
                     /translation="GHSYSTQALVGGDAQLAARFEDGHFATLYLSPKDYHRIHMPCDG
                     TLERMIHVPGSLFSVNPVTARGVPGLFARNERVVCVFESAAVGRFVLVLVGATIVGSM
                     ATAWHGTVNAPRVRRVREWHYPAGDVSLAKGAEMGRFMLGSTVVLLFSREAGLQFAAD
                     WQPGRAIRLGEAMATATPPVAPSR"
     gene            568..1500
                     /locus_tag="VFF72_10245"
     CDS             568..1500
                     /locus_tag="VFF72_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV93585.1"
                     /translation="MRAVFVDANAALRTLAERIGPRTVPELAIGFGAPGPDPSTLPSL
                     LGGAGIAVIDHTALPIDVARRCVGLRDVVFLGTGARSYMDPEALAGIGISVHVIKGYG
                     DTAVAECTIGLLFAAARGFAAMDSAMRRGQWRRSEGMQLGGKTLGLIGFGGIAAEVAR
                     LAQGLGMKVLAWNRSARAFPGVEFVAVERLLAESDAVSMHLLLTDETRGFLTRERIAA
                     LKPGVLLVNTARGALVDEAAMIDALETGRIGHAGLDVFETEPLPAGHPLAALDNVTLS
                     AHSGFRTPEANERLVEAAFAHCRRIVDDTARSPA"
     gene            1604..2233
                     /locus_tag="VFF72_10250"
     CDS             1604..2233
                     /locus_tag="VFF72_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV93586.1"
                     /translation="MKGTSRSGQRRSDRHAVETRRSAQKAESRERIVRAAAASVRREG
                     FAGTGVAEVMGEAGLTHGGFYAHFPSREALLAAAADGAGADGVAQLARIAAAAPAGGG
                     LAAIAEAYLSDRHVERAERGCPIVAVGSELARQAPSVRHAATGRIKEMVDLIERQDSG
                     WGEAGGHARALARLSCMVGAMVLARAVDDPDLSRAVREAASDAITGTSR"
     gene            2306..3814
                     /locus_tag="VFF72_10255"
     CDS             2306..3814
                     /locus_tag="VFF72_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV93587.1"
                     /translation="MKTSLPAVAIAPASVVAAPSATIPGALAVPTGAAPTSAGSISGA
                     AAPAATGSPWLVFFVASIAVFLVAIDSTVLYAAFAALRRAFPEARAADLSWVLNAYTV
                     VYAALLVPSGRLADLYGRKRIFATGVSIFLLASLACGLSTHVALLIAARVAQAVGAAL
                     LTPASLAIVLAAFPASRRAVVVAAWGAVGGLAAALGPSVGSWVVDAWGWPWAFFINLP
                     LGALSLWRGRGALAESRNPENAAPLDIVGVLLLIGGVGAIAFGLVRIDALGWDSPVVL
                     GCAAAGAALLACFVAWARHAASPAIDLTLFDSPTYRWVNLATLSFGIAFAMMFFGFFL
                     FMTAVWHFSLPEAGLAITPGPLMVVPTAIVSGRIAARIGHRPLLVVGSVVYACGGLWF
                     RLVPGIAPDYLHHWLPGLFLTGIGVGMVLPSLSGAAVAQLAPNRFGIGSAVNQAVRQV
                     GSVLGVAVTIVLVGKTAPQLVDFHHLYDGHVALALLTALLCLRVQTRPRAPA"
     gene            complement(3860..>4296)
                     /locus_tag="VFF72_10260"
     CDS             complement(3860..>4296)
                     /locus_tag="VFF72_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZV93588.1"
                     /translation="LAEQPAERDRLIAEPGLIRSAVEEFLRIESSNQLGNRITTRATA
                     IAGEPLEPRTQVTLCIGAANRDPEVFPEPDRLDISRHPNRHVAFGSGIHQCVGMGLGR
                     LEAKIAIGRFVARFPRYALAGRPERSRRVRFRGHVRLPVVLA"
BASE COUNT          508 a         1584 c         1526 g          678 t
ORIGIN      
        1 aggccacagc tactcgacgc aagccttggt gggtggtgac gcgcaactgg ccgcacgctt
       61 tgaagacggc catttcgcta cgctgtacct gagccccaag gactaccacc gcatccacat
      121 gccgtgcgac ggcacgctgg agcggatgat ccacgtgccg gggtcgctgt tctccgtgaa
      181 cccggtgacc gcgcgcggcg tgccggggct cttcgcgcgc aacgagcgcg tcgtctgcgt
      241 cttcgagtcg gccgcggtcg gccggttcgt gctcgttctc gtcggcgcca ccatcgtcgg
      301 cagcatggcg acggcgtggc acggcacggt caacgcgccg cgcgtgcgtc gcgtgcgcga
      361 atggcactat ccggccggcg acgtctcgct ggcgaagggc gccgaaatgg ggcggttcat
      421 gctcggctcg acggtcgtgc tgctgttctc gcgtgaagcc ggcctgcagt tcgcggccga
      481 ctggcagccc ggccgcgcga tccgcctcgg cgaagcgatg gcgacggcaa cgccgccggt
      541 cgcgccctca cgctgaaggg ccgccgaatg cgcgccgtct tcgtcgacgc caacgccgcg
      601 ttgcgcacgc tggccgagcg catcggcccg cggacggtgc ccgagctcgc gatcggcttc
      661 ggcgcgcccg ggcccgatcc ctcgacgctg ccgtcgctgc tgggcggcgc cggcatcgcc
      721 gtcatcgacc acacggcctt gccgatcgac gtggcaaggc gctgcgtcgg cctgcgcgac
      781 gtggttttcc tcggcaccgg cgcgcgcagc tacatggacc ccgaggcgct ggcaggcatc
      841 ggcatctcgg tgcacgtcat caagggctac ggcgacaccg ccgtcgccga atgcacgatc
      901 gggctgctct ttgccgcggc acgcggcttc gccgccatgg acagcgccat gcgacgcggc
      961 cagtggcgcc gcagcgaagg catgcagctc ggcggcaaga cgctcggcct gatcgggttc
     1021 ggcggcatcg ccgccgaggt cgcccggctg gcccaggggc tcggcatgaa ggtgctggcc
     1081 tggaaccgca gcgccagggc ctttccgggc gtcgagttcg tcgccgtcga gcgacttctc
     1141 gccgagagcg acgccgtgtc gatgcacctg ctccttaccg acgagacacg cggctttctg
     1201 acgcgcgagc gaatcgccgc gctgaagccg ggcgtgctgc tcgtcaatac cgcgcgcggc
     1261 gcgctggtcg acgaagccgc gatgatcgac gcgctcgaga cgggccgcat cggccacgcc
     1321 ggcctcgacg tgttcgagac cgagccgctg ccggccgggc atccgctcgc cgcgctggac
     1381 aacgtcacgc tgtcggccca ttcggggttc cggacgcccg aggcgaacga gcgcctcgtc
     1441 gaggcggcgt tcgcgcactg ccggcgcatc gtcgacgaca cggctcgatc gccggcctga
     1501 tccaccctcc tggcctcccg gcctgaaacg ggcacgggcc cgagacgcct ctcgcatggc
     1561 gtgtcgagca tcggttccag aaccgtcgta tgatgggcga catatgaaag gcacttcacg
     1621 ttccggccag cgtcgatcgg atcgccacgc cgtcgagacg cgacgcagcg cgcagaaggc
     1681 ggagtcgcgc gaacgcatcg ttcgcgccgc cgccgcctcg gtgcgtcgcg aaggcttcgc
     1741 cggcaccggc gtcgccgagg tcatgggcga ggccggcctc acccatggcg gcttctatgc
     1801 ccacttcccg tcgcgcgagg cacttctcgc cgctgccgcc gacggtgccg gcgccgacgg
     1861 cgtggcgcag ctggcgcgaa tcgccgcagc ggcgccggcg ggcggcggcc tcgcggcgat
     1921 cgccgaggcc tatctgtccg atcgccacgt cgagcgcgcc gagcgcggct gcccgatcgt
     1981 cgccgtcggc tcggagctgg cgcgccaagc gccttcggtg cgccacgcgg ccaccggccg
     2041 catcaaggag atggtcgacc tgatcgagcg ccaggacagc ggctggggcg aagccggcgg
     2101 ccatgcccgg gcgctggccc ggctgagctg catggtgggg gccatggtcc tggcccgcgc
     2161 cgtcgacgac cccgatctgt cgcgcgccgt ccgcgaagcg gcgagcgacg cgatcaccgg
     2221 cacctctcgc tgagccggct tcgcagcaag ccgtttcgcc gccacgtcca tttcatatga
     2281 caagcaacat ccgggatacg tcgacatgaa aacgtccttg cctgccgtgg cgatcgcgcc
     2341 agcgtccgtc gtcgcggcgc cgtccgcgac gatcccggga gccctggcgg tgccgacggg
     2401 cgcagcgccg acgtccgcag gatcgatttc gggcgccgcg gcgccggcgg cgacgggctc
     2461 gccctggctg gtcttcttcg tcgccagcat cgccgtcttc ctggtggcga tcgactcgac
     2521 cgtgctctac gcggcgttcg ccgcgttgcg ccgcgccttt cccgaggcgc gggcggccga
     2581 cctctcgtgg gttctcaacg cctacacggt cgtctatgcg gcgctgctcg tgccgtcggg
     2641 gcgactggcc gatctctacg gccgcaagcg catcttcgcc accggcgtct cgatcttcct
     2701 gctcgcctcg ctggcctgcg gcctgtcgac gcacgtcgcc ctgctgatcg cggcgcgcgt
     2761 cgcgcaggcg gtgggcgcgg cgcttctcac gcccgcctcg ctcgccatcg tgctggcggc
     2821 gtttccggcg tcgcgccgtg ccgtcgtcgt cgccgcctgg ggcgccgtcg gcgggctggc
     2881 cgcggcactc ggcccgagcg tcggctcgtg ggtcgtcgac gcctggggct ggccgtgggc
     2941 gttcttcatc aacctgccgc tgggcgcgct gtcgctgtgg cgcggccgtg gcgcgctcgc
     3001 cgaatcgcgc aatcccgaga acgcggcgcc gctcgacatc gtcggcgtgc tgctgctgat
     3061 cggcggcgtc ggcgcgatcg ccttcggcct cgtgcggatc gacgccctcg gctgggactc
     3121 gcccgtggtg ctcggctgcg ccgcggccgg cgccgccctg ctcgcctgct tcgtcgcctg
     3181 ggcgcgccac gccgcgtcac cggcaatcga cctcaccctc ttcgacagcc cgacctatcg
     3241 ctgggtcaac ctcgcgacgc tcagcttcgg catcgccttc gcgatgatgt tcttcggctt
     3301 tttcctgttc atgacggcgg tctggcattt ctcgctgccc gaggcgggcc tggcgatcac
     3361 gcccgggccg ctgatggtgg tgccgacggc gatcgtctcg ggccgcatcg ccgcgcgaat
     3421 cgggcatcgg ccgctgctcg tcgtcggcag cgtcgtctat gcctgcggcg gcctctggtt
     3481 tcgcctcgtt cccggcatcg cccccgacta tctgcaccac tggctgccgg gcctctttct
     3541 gaccggcatc ggcgtcggca tggtgttgcc gtcgctctcg ggcgcggccg tcgcgcagct
     3601 cgcacccaac cgcttcggca tcggcagcgc ggtcaaccag gcggtgcgcc aggtcggctc
     3661 ggtgctcggc gtcgcggtga cgatcgtcct ggtcggcaag accgcgccgc aactcgtcga
     3721 ctttcaccat ctctacgacg gccacgtcgc gctggcgctg ctcaccgcgc tgctgtgcct
     3781 gcgcgtgcag acgcggccgc gcgcccccgc ctgagcgtcg gcgcgcggcc ggcgccgcac
     3841 gcgcgaccga cccgcgcgct caggccagca cgacgggcag ccgcacgtgg ccgcgaaagc
     3901 ggacccgccg gctgcgctcg ggccgcccgg cgagcgcgta gcgcggaaag cgcgcgacaa
     3961 agcggccgat ggcgatcttc gcctcgagac ggccgagccc catgccgacg cactggtgga
     4021 tgcccgagcc gaaggcgacg tgacgattgg gatggcgcga gatgtcgagg cggtcgggtt
     4081 cgggaaagac ctcggggtcg cggttggccg cgccgatgca cagcgtcacc tgggtgcgcg
     4141 gttcgagcgg ctcgccggcg attgcggtgg cacgcgtcgt gatccggttg cccagctggt
     4201 tggagctctc gatgcgcagg aattcctcga cggcgctgcg gatcaggcct ggctcggcga
     4261 tcaggcggtc gcgctcggcc ggctgctcgg ccaggc
//
LOCUS       DASRWS010000544         6212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_225604, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000544 DASRWS010000000
VERSION     DASRWS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225604"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..777
                     /locus_tag="VFF72_10265"
     CDS             70..777
                     /locus_tag="VFF72_10265"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZV93589.1"
                     /translation="MSSKRTFKIYRYDPETGAAPSMQTIEIELDGSERMLLDVLIKLK
                     AQDPSLSFRRSCREGVCGSDAMNINGKNGLACLTNMRTLKDPVVLKPLPGLPVVRDLI
                     VDMTDFFKQYHSIKPYLVNDEMPPEKERLQSPEERDELNGLYECILCASCSSSCPSFW
                     WNPDKFVGPAGLLQAYRFLADSRDQATGERLDNLEDPYRLFRCHTIMNCVDVCPKGLN
                     PTRAIGKIKELMVRRAV"
     gene            789..1151
                     /locus_tag="VFF72_10270"
     CDS             789..1151
                     /locus_tag="VFF72_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HZV93590.1"
                     /translation="MAPSSEIGRLVDAGESSGVPIDERALSRLRWRCRRGMLENDLLI
                     ERFFARRGVVDEADRIGMTSLMALGDNDLLDLFLGRQEPIAELDTPEVRQVLAAVRPT
                     RPSHASLPSTANPSRTLP"
     gene            1148..2458
                     /gene="gltA"
                     /locus_tag="VFF72_10275"
     CDS             1148..2458
                     /gene="gltA"
                     /locus_tag="VFF72_10275"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZV93591.1"
                     /translation="MIPSDTKATLSFSDGSPSMDLPIYKGTIGPDVIDIRKLYGQTGK
                     FTFDPGFMSTASCDSTITYIDGDKGELLYRGYPIEQLATTCDYLETCYLLLKGELPTR
                     SQKDDFDDLVTHHTMVNEQMQFFLRGFRRDAHPMAIMTGLVGAMSAFYHDSTDIHNAQ
                     HREISAIRLIAKMPTLVAMTYKYSAGQPYVYPKNDLSYAGNFMRMMFATPCEEYEPNE
                     VLVRAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAA
                     LNMLEEVQRMGGVEKIGEFIKQVKDKNSGIKLMGFGHRVYKNYDPRAKLMRETCHEVL
                     DTLGLHDDPIFKLAMSLEKIALEDEYFVARKLYPNVDFYSGIVQRAIGIPVSLFTAVF
                     ALARTVGWIAQLNEMIADPDYKIGRPRQLFTGARPRQVVPMTQR"
     gene            2493..3905
                     /gene="leuC"
                     /locus_tag="VFF72_10280"
     CDS             2493..3905
                     /gene="leuC"
                     /locus_tag="VFF72_10280"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZV93592.1"
                     /translation="MNPRTLYDKLWDEHVVRTEDDGTALLYIDRHLVHEVTSPQAFEG
                     IEQAGRKVWRVSANLAVSDHNVPTTDRSHGIADPISRLQVDTLDANCDRWGVTQFKMN
                     DLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLL
                     ARKAKNMLVKVEGRLPRGCTAKDIVLAVIGRIGTAGGTGYTIEFAGSAVRALSMEGRM
                     TVCNMAIEAGARAGLVGVDETTINYVKGRPYSPTGLEWEAALRHWKTLHSDAGAHWDR
                     VVEMDAATLVPQVTWGTSPEMVTGIEGRVPDPEREKDAGKRSAMERALQYMALEPNQR
                     IDDIRIDKVFIGSCTNSRIEDLREAAAIVRRLGRRVAGNVRLAMVVPGSGLVKKQAES
                     EGLDAVFRAAGFEWREAGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVS
                     PAMAAAAAIEGHFVDVRRLA"
     gene            3902..4549
                     /gene="leuD"
                     /locus_tag="VFF72_10285"
     CDS             3902..4549
                     /gene="leuD"
                     /locus_tag="VFF72_10285"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZV93593.1"
                     /translation="MTPFTVHSGLVAPMDRENVDTDAIIPKQFLKSIRKSGFGPNLFD
                     AWRYLDPGEPGQDPASRRPNPDFVLNQPRFKGASILLARKNFGCGSSREHAPWALQQY
                     GFRAILAPSYADIFFNNCFKNGLLPIVLGEHQTSSLFDEVAAFVGYSLTIDLQRQVVV
                     RPGGETLAFEVEPFRKFCLLGGYDDIALTLRHAEAIKTFEAERLLRQPWLATTIR"
     gene            4632..5729
                     /gene="leuB"
                     /locus_tag="VFF72_10290"
     CDS             4632..5729
                     /gene="leuB"
                     /locus_tag="VFF72_10290"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZV93594.1"
                     /translation="MKIAILPGDGIGTEIVAQAERVLASLDLRFETEHALVGGAAYET
                     HGHPLPEATLRLARAADAILFGAVGDWKFDRLERSLRPEQAILGLRKSLGLFANFRPA
                     ICYPELTAASSLKAELVGGLDILIIRELTGDIYFGQPRGRRASPDGAFVGAEEAFDTM
                     RYSRPEIERIAHVAFRAAQKRSKRLTSVDKANVLETFQLWKDVVTDVHTQYPDVELDH
                     MYVDNAAMQLVRAPKKFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDARGKGLYE
                     PSHGSAPDIAGKDLANPLATILSLAMMLRFSLEQPEAAARIEQAVQAVLASGLRTADI
                     WSEGGTKVGTRQMGDAVVAALAAQKTAITKS"
     gene            5813..>6212
                     /locus_tag="VFF72_10295"
     CDS             5813..>6212
                     /locus_tag="VFF72_10295"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZV93595.1"
                     /translation="MELTGLVGWRGMVGSVLLERMQAEGDFDLIEPLFFSTSDKGGRA
                     PSQAKNERTLQDAYDIEALRRCAVIVTAQGGDYTKDVYPRLRSAGWNGYWIDAAKTLR
                     MHDDAVIVLDPVNLDVIRSAMTRGVRNYIGG"
BASE COUNT         1112 a         2106 c         2014 g          980 t
ORIGIN      
        1 tcaagtcgca gccgctgacc gtcgagtcga ttccgccgaa agtgcgcacg ttctgacgcg
       61 caagacagca tgagcagcaa acgcaccttc aagatctacc gctacgaccc cgagaccggc
      121 gcggcgccct cgatgcagac catcgagatc gagctcgacg gcagcgagcg gatgctgctc
      181 gacgtgctga tcaagctgaa ggcccaggat ccgagcctca gcttccgccg ttcgtgccgg
      241 gaaggcgtct gcggctcgga tgcgatgaac atcaacggca agaacggcct ggcctgtctc
      301 accaacatgc gcacgctcaa ggaccccgtg gtgctcaagc cgctgcccgg cttgccggtg
      361 gtgcgcgacc tgatcgtcga catgacggac ttcttcaagc agtaccactc gatcaagccg
      421 tacctcgtca acgacgagat gcctcccgag aaggagcggc tgcaaagccc cgaggagcgc
      481 gacgagctca acggcctcta cgagtgcatc ctctgcgcca gctgctcgag ctcgtgcccg
      541 agcttctggt ggaatcccga caagttcgtc ggcccggcag gcctgctcca ggcctatcgc
      601 ttcctcgccg acagccgcga ccaggccacc ggcgaacgcc tcgacaacct cgaggatccg
      661 taccgcttgt tccgctgcca caccatcatg aactgcgtcg acgtctgccc gaagggcctc
      721 aatccgaccc gggccatcgg caagatcaag gagctgatgg tgaggcgcgc cgtctgatgc
      781 aggaagagat ggccccctcc agcgagatcg ggcggcttgt cgacgcgggc gagtcgtcgg
      841 gcgtgccaat cgacgagcgg gcgctcagcc ggctgcgctg gcgctgccgg cgcggcatgc
      901 tcgagaacga cctgctgatc gagcgcttct tcgcccggcg aggcgtcgtc gacgaggccg
      961 accggatcgg catgacctcg ttgatggcgc tgggcgacaa cgatctgctc gacctgtttc
     1021 tcggccgcca ggaaccgatt gcggagctcg acacgccgga ggtccggcaa gtgctcgcgg
     1081 ccgtccgccc gacgaggccg tcgcacgcct cgctcccttc taccgccaat ccttcgagga
     1141 cattgccatg atcccgtccg acaccaaagc caccctctcg ttctcggacg gcagccccag
     1201 catggatctg ccgatctaca agggcacgat cggccccgat gtcatcgaca tccgcaagct
     1261 ctacgggcag acgggcaagt tcaccttcga tccgggtttc atgtcgaccg cctcgtgcga
     1321 ctcgacgatc acctacatcg acggcgacaa gggcgagttg ctctaccgcg gctatccgat
     1381 cgagcagctc gcgaccacct gcgactacct cgagacctgc tacctgctcc tcaaggggga
     1441 actgccgacg cgaagccaga aggacgactt cgacgatctg gtgacgcatc acaccatggt
     1501 caacgagcag atgcagttct tcctgcgcgg ctttcgccgc gacgcccatc cgatggcgat
     1561 catgacgggg ctggtcggcg cgatgtcggc cttctatcac gactcgaccg acatccacaa
     1621 cgcgcagcac cgcgagatct cggcgatccg cctgatcgcc aagatgccga cgctggtggc
     1681 gatgacctac aagtattcgg ccggccagcc ctacgtctat ccgaagaacg acctctcgta
     1741 cgccggcaac ttcatgcgca tgatgttcgc gacgccgtgc gaggagtacg agccgaacga
     1801 agtgctggtg cgggcgatgg atcgcatctt catcctccat gccgatcatg agcagaacgc
     1861 gtcgacatcc accgttcgac tctgcggctc gtcgggcacc aacccgttcg cggccatcgc
     1921 cgcgggcgtc gcctgcctgt ggggtcccgc gcacggcggc gccaacgagg cggcgctcaa
     1981 catgcttgag gaagtgcagc ggatgggagg cgtcgagaag atcggcgagt tcatcaagca
     2041 ggtgaaggac aagaactcgg gcatcaagct gatgggcttc ggccaccggg tctacaagaa
     2101 ctacgatccg cgcgccaagc tgatgcgcga aacctgtcac gaggtgctcg acacgctcgg
     2161 cctgcacgat gacccgatct tcaagctggc gatgtcgctc gagaagatcg cgctggagga
     2221 cgaatacttc gtcgcccgaa agctctaccc gaatgtcgat ttctactcgg ggatcgtgca
     2281 gcgggcgatc ggtattccgg tgagcctctt caccgccgtc ttcgccctcg cgcgcacggt
     2341 cggctggatc gcgcagctca acgaaatgat cgccgacccc gactacaaga tcggccggcc
     2401 gcgccagctc ttcaccgggg cgcgtccgcg gcaggtcgtg ccgatgaccc agcgatgaag
     2461 ccggccgacg cggccggcgc cgaggagcat ccgtgaatcc gcgcactctc tacgacaagc
     2521 tctgggacga gcacgtcgtc cgcaccgagg acgacggtac cgcgctgctc tacatcgacc
     2581 gtcacctcgt gcacgaggtg acgagcccgc aggcgttcga gggcatcgag caggctggcc
     2641 gcaaggtgtg gcgggtctcg gccaacctcg cggtcagcga ccacaacgtg ccgacgaccg
     2701 atcgatcgca cggcatcgcc gacccgatct cgcggttgca ggtcgacacc ctcgacgcca
     2761 attgcgaccg ctggggcgtg acgcagttca agatgaacga cctgcgccag ggcatcgtcc
     2821 atgtcatcgg ccccgagcag ggcgcgacgc tgcccggcat gacggtggtc tgcggcgact
     2881 cgcacacctc cacgcacggc gccttcggcg cgctggcgca cggcatcggc acgagcgagg
     2941 tcgagcatgt gctggcgaca cagaccctgc tggcgagaaa ggcgaagaac atgctggtca
     3001 aggtggaggg tcgcctgccg cgcggctgca ccgccaagga catcgtgctg gccgtgatcg
     3061 gccggatcgg cacggccggc ggcaccggct acaccatcga attcgcggga agcgcggtgc
     3121 gcgcgttgtc gatggaaggc cggatgacgg tctgcaacat ggcgatcgaa gcgggagcgc
     3181 gggccggcct ggtcggcgtc gacgagacga cgatcaacta cgtgaagggc cgcccgtatt
     3241 cgccgaccgg cctggaatgg gaggcggcgc tgcgccactg gaagacgctg cactccgatg
     3301 ccggcgcgca ttgggatcgg gtcgtcgaga tggacgcagc cacgctggtg ccgcaggtca
     3361 cctggggaac gtcgccggag atggtgacgg gcatcgaggg ccgtgtgccc gaccccgagc
     3421 gcgagaagga cgccggcaag cgcagcgcca tggaacgggc cctgcaatac atggcgctcg
     3481 agccgaacca gcgcatcgac gacatccgca tcgacaaggt cttcatcggc tcgtgcacga
     3541 actcgcgcat cgaggatctc cgcgaagccg cggccatcgt ccgccggctc ggtcgtcgcg
     3601 tcgctggcaa cgtcaggctg gcgatggtcg tcccgggttc ggggctggtg aagaagcagg
     3661 ccgaaagcga gggcctcgat gccgtctttc gcgcggccgg attcgagtgg cgcgaggcgg
     3721 gctgctcgat gtgcctggcg atgaatgccg atcgactgga gccgggcgag cgctgcgcgt
     3781 cgaccagcaa tcgaaacttc gaaggacgcc agggcgcggg cggccgcacc catctcgtca
     3841 gcccggcgat ggccgccgcc gcggccatcg agggccattt cgtcgacgtg aggcgattgg
     3901 catgacgccc tttaccgttc actccggcct cgtcgcgccg atggatcgcg agaacgtcga
     3961 caccgacgcc atcattccga agcagttcct caagtcgatc aggaagtcgg gcttcggccc
     4021 gaacctgttc gacgcctggc gctacctcga tccgggcgag ccgggccagg atccggcatc
     4081 gcggcgaccg aatccggact tcgttctcaa ccagccgcgc ttcaagggcg cgtccatcct
     4141 cctggcacgc aagaacttcg gctgcggttc gtcgcgcgag catgcgccat gggcgctcca
     4201 gcaatatggc tttcgcgcca tcctcgcgcc cagctacgcc gacatcttct tcaacaactg
     4261 cttcaagaac ggcctgttgc cgatcgtcct cggcgagcac cagacgagtt cgctcttcga
     4321 cgaggtggcg gccttcgtcg gctactcgct gacgatcgat ctgcagcgtc aggtcgtggt
     4381 gcggcccggc ggcgagacgc tggccttcga ggtcgagccg tttcgcaagt tctgtcttct
     4441 cggcggctac gacgacatcg cgctcaccct gcgccatgcc gaagcgatca agaccttcga
     4501 ggcggaacgc ctcttgaggc agccgtggct cgcgacaacg attcgatgat gcgcacgccc
     4561 tcgtgccgga acgaccggcg ggtcgcgatc gaggcgtgcg ttgagcggcg cccgccggtg
     4621 cgggggacat gatgaagatc gccatccttc ccggcgacgg catcgggacc gagatcgtcg
     4681 cgcaggccga gcgggtcctg gcgtcgctgg accttcgctt cgagacggag cacgcgctgg
     4741 tcggcggcgc cgcctacgag actcacggcc atccgctgcc ggaagcgacg ctgcggctgg
     4801 cccgggccgc cgacgcgatc ctcttcggcg ccgtcggcga ctggaaattc gaccggctcg
     4861 agaggtcgct gcgtcccgag caggcgatcc tcggcctgcg caagtcgctc ggcctgttcg
     4921 ccaacttccg tccggcgatc tgctacccgg agctcaccgc ggcgtcgagc ctgaaggcgg
     4981 agctcgtcgg cggcctcgac atcctcatca tccgcgagct caccggcgac atctatttcg
     5041 gccagccacg tggccgacgc gcgtcgcccg acggcgcgtt cgtcggcgcc gaggaagcgt
     5101 tcgacacgat gcgctattcg cgccccgaga tcgagcggat cgcccacgtc gcctttcgcg
     5161 cggctcaaaa gcggagcaag cggctgacga gcgtcgacaa ggccaacgtg ctcgagacgt
     5221 tccagctctg gaaggatgtc gtcaccgacg tccacacgca gtaccccgac gtcgagctcg
     5281 accacatgta cgtcgacaac gccgcgatgc aactggtgcg tgcgcccaag aagttcgacg
     5341 tcgtcgtcac cggcaacatg ttcggcgaca tcctgtccga cgaagcggcg atgctcacgg
     5401 gctcgatcgg aatgctgcca tcggcttcgc tcgatgcgcg gggcaagggc ctctacgagc
     5461 ccagccacgg cagcgctccg gacatcgccg gcaaggacct ggcgaacccg ctggctacaa
     5521 tcctgtcgct tgccatgatg cttcgcttct cactcgaaca acccgaggcc gccgcgcgga
     5581 tcgagcaggc cgtgcaggcc gtgctcgctt cgggcttgcg cacggccgac atctggtcgg
     5641 agggtggcac gaaggtcggc acgcggcaga tgggcgacgc cgtcgtggcg gcgctcgctg
     5701 cgcagaaaac ggcgatcacc aagagctagg caacaccgcg tcgtcgcgcc ctctccccgg
     5761 ccggcggccg gcggacccga aggaatcaat tcacttcagt cagggcaagg cgatggaact
     5821 cacaggattg gtcggctggc gcgggatggt cggctccgtg ctgctcgagc gcatgcaggc
     5881 cgaaggcgat ttcgacctga tcgagccgct cttcttcagc accagcgaca agggcggccg
     5941 ggcgccgtcg caagcgaaga acgagcgcac gctgcaggac gcctacgaca tcgaggcgct
     6001 caggcgttgc gccgtcatcg tcaccgcgca aggcggcgac tacacgaagg atgtctaccc
     6061 gcggcttcgg tctgccggct ggaacggcta ctggatcgac gcggccaaga cgctgcgcat
     6121 gcacgatgac gccgtcatcg tcctcgaccc cgtcaatctc gatgtcatcc gttcggcgat
     6181 gacgcggggc gtgcgcaact acatcggcgg ca
//
LOCUS       DASRWS010000545         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3669607, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000545 DASRWS010000000
VERSION     DASRWS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669607"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFF72_10300"
     CDS             complement(<1..221)
                     /locus_tag="VFF72_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZV93596.1"
                     /translation="MALAALNDATSAARREIMVREQIERRGVRDPAVLEAMRQVPREA
                     FVPAARRERAWDDTPLPIAEGQTISQPYIV"
     gene            complement(247..1404)
                     /locus_tag="VFF72_10305"
     CDS             complement(247..1404)
                     /locus_tag="VFF72_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93597.1"
                     /translation="MRVASILPLLAACLIGACAVAPPAPRAPPALFADGRFGPPDEAI
                     STDDVFALSDEMRHYLAVDIADQLRVEGAQAGLIDALYRKSQLHLEYDSTRTKNAAET
                     FASRSGNCLSLIVMTAALAHALDIPVIFQSASIDPTWSRSGDLLFSSGHVNVTLGQRL
                     RDANSIRDLSETTVDFLPPGELGRMRTREISESTVLAMYANNRAAEAMARGRLDDAYA
                     WSALALRKDPGYLAAFNTLGVVYMRHGDVALATQVFTHVLSREPRDTRAMANLAESDD
                     RLGRKAEAAALRVRLAALETNPPFHFFSLGLEAMRRADYAAARDYFKREVDRADYYHE
                     FHFWLGLADWHLGDVVQAKRQLALAMDNSTTRGQHDLYAAKLQWLEAQRHQ"
     gene            complement(1465..1839)
                     /locus_tag="VFF72_10310"
     CDS             complement(1465..1839)
                     /locus_tag="VFF72_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZV93598.1"
                     /translation="MPLTCFIRYEIDPFQRDAFKTYAERWGRIIPRCGGHLVGYFLPH
                     EGSNDIAWGLIAFDSLADYEAYRARLRTDAEGRANFEFAQAGRFIRREERSFVEVVPG
                     TFGVAAMPGEPASVDHGPADAS"
     gene            2068..2418
                     /locus_tag="VFF72_10315"
     CDS             2068..2418
                     /locus_tag="VFF72_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93599.1"
                     /translation="MTTHRHRPVSQRRFAWLFWLALVLPLAQSAASWHAFSHLREAPP
                     VDADAGKHALHAAHCGLCLMAAAIAGGAPPAEPPVVAQGPIRHATPASRIVAALHLVA
                     PDAYLSRAPPLVSL"
     gene            2490..3962
                     /locus_tag="VFF72_10320"
     CDS             2490..3962
                     /locus_tag="VFF72_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93600.1"
                     /translation="MHKLVPATFALALILPFGARADTAADVAALRQEMDAMRASYEAR
                     LESLEERLRRTEAALPVAAASAPPAAMAANASPVVVARSSAAPPAVAATAALPAVAAT
                     VVPPPPDTGSGSGGLNAFNPSLSLILSGLYARTSQDPSRYAITGFPLPPDAQIGPGTR
                     GFSLDESELGVAASIDPWLRGAANIAFEADNSVSIEEAFVQTTSLGHGVSLKAGRFFS
                     GIGYLNPQHSHTWDFVDNPLAYQAMLGTQYDDDGVQLMWLPPIDQYVEIGAELGRGRS
                     YPGTDTGRNGAGMNSIFAHTGGDIGDSQSWRAGVSMLDAKASAQQLRATDTLGHVVTN
                     AFDGSTRVWIADAIWKWAPHGNATRTNFKLQGEYLHSTRSGNLAYDIDGIDSAGAYRA
                     AQSGWYVQGVWQFMPYWRVGLRTERLDPGTPDYGLNSDVLATSGFHPHKNTLMVDFNP
                     SEFSRVRLQFARDQSREGVADNEVFLQYQMSLGAHGAHSY"
     gene            3988..>4310
                     /locus_tag="VFF72_10325"
     CDS             3988..>4310
                     /locus_tag="VFF72_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZV93601.1"
                     /translation="MNPRFHSILTLLAAPILFLALPAQAALHVFACEPEWGALAQELG
                     GKLVDVSVATNALQDPHQIQAKPSLIARARSADLVVCTGAELEVGWLPVLLQQSGNGK
                     VQPGQP"
BASE COUNT          630 a         1515 c         1470 g          695 t
ORIGIN      
        1 acgatgtagg gctgcgagat cgtctgtcct tcggcgatcg gcaagggcgt gtcgtcccag
       61 gcacgttcgc gccgggccgc cggcacgaag gcctcgcgcg gcacctgccg catcgcctcg
      121 agcaccgccg ggtcgcgcac gccgcgccgc tcgatctgct cgcgaaccat gatctcccgg
      181 cgcgccgccg atgtcgcatc gttcagggcc gcaagcgcca cgtcgggtgc gccagtcgac
      241 tcgcctctac tggtgccgct gcgcctccag ccactgcagc ttggcggcgt agaggtcgtg
      301 ctgtccgcgc gtcgtgctgt tgtccatggc gagcgcgagc tgccttttcg cctggacgac
      361 atcgccgaga tgccagtcgg ccaggccgag ccagaaatgg aactcgtggt aatagtcggc
      421 ccggtcgacc tctcgcttga agtagtcgcg cgccgcggcg tagtcggcac ggcgcatcgc
      481 ctccaggccg aggctgaaga agtggaacgg cgggttcgtc tccagcgccg cgagtcgcac
      541 acgcagcgcc gccgcctcgg cctttcgacc gagacggtcg tcgctctcgg cgaggttggc
      601 cattgcccgg gtgtcacgcg gctcgcggct caggacgtgc gtgaagacct gggtcgccag
      661 ggcgacgtcg ccgtggcgca tgtagacgac tcccagcgtg ttgaatgccg cgaggtagcc
      721 cgggtccttg cgcagcgcga gcgccgacca ggcgtaggca tcgtcgagtc ggccgcgcgc
      781 catcgcctcg gcggcgcggt tgttcgcgta catcgccagc acggtcgatt ccgagatctc
      841 tcgcgtccgc atgcggccga gctccccggg cggcaggaag tcgaccgtcg tttcactcag
      901 gtcgcggatg ctgttcgcgt cgcgcaagcg ctggccgagc gtgacgttga cgtgtcccga
      961 ggagaacagc aggtcgccgc tgcggctcca ggtcgggtcg atggacgcgc tctggaagat
     1021 caccgggatg tcgagcgcat gcgccagcgc cgctgtcatg acgatgagcg agaggcagtt
     1081 gccggaccgg ctggcgaagg tctcggcggc attcttggtg cgcgtcgagt cgtattcgag
     1141 gtggagttgc gacttgcgat agagcgcgtc gatcaggccg gcctgcgcgc cctcgacgcg
     1201 caactggtcg gcgatgtcga cggcgaggta gtgcctcatc tcgtcgctga gcgcgaagac
     1261 gtcgtcggtc gatatggctt cgtcgggcgg cccgaaccgg ccgtcggcaa aaagggccgg
     1321 cggggcccgc ggcgccggcg gcgccacggc gcaggcgccg atcaggcagg cagcgagcaa
     1381 aggcaggatc gaggcgacac gcatcggctt ctccccgcgg cggcgaggca tctgccgccg
     1441 cgacaactca accgggagcc cgcatcatga cgcatcggcg gggccgtggt cgaccgacgc
     1501 cggctcgccg ggcatcgcgg cgacgccgaa ggtgccgggg acgacttcga cgaagctgcg
     1561 ctcctcgcgc cggatgaacc tgccggcttg ggcgaactcg aagttggccc gcccttcggc
     1621 atcggtcctg aggcgggccc ggtaggcttc gtagtcggcg agcgaatcga aggcgatcag
     1681 cccccaggcg atgtcgttgc tgccttcgtg cggcaggaag tagccgacga ggtggccgcc
     1741 gcaacgcgga atgatgcggc cccagcgctc ggcgtaggtc ttgaaggcgt cgcgctggaa
     1801 cgggtcgatc tcgtagcgga tgaagcaggt gagcggcatg tgcggtctcg aggaagggac
     1861 gccgacagcg tagccgcgga tcgggccgac atggttcggc gctgctcgaa tcatggagac
     1921 gggttgccgg catcgatgcc ctcccggcga ttgcgcgacg gcgccgcgtg aatggcgcct
     1981 cgtcgggcgc ctcgtctttc cgatttcagg cgacgttcac gttcggaaac cttcgccgcc
     2041 gggcgcggga ctaagatggt tgcccgcatg acgacgcacc gtcaccgtcc cgtttcgcag
     2101 cgccgcttcg cctggctgtt ctggctggcg ctggtgttgc cattggcgca gtcagcggcg
     2161 tcgtggcatg ccttctcgca tctgcgcgag gcgccgccgg tcgacgccga cgccggcaag
     2221 cacgcgttgc atgcggctca ctgcggcctg tgcctgatgg ccgcggcgat cgccggaggc
     2281 gcgccgccgg ccgagccgcc ggtcgtggcg cagggtccga tccggcatgc gacgccggcg
     2341 agccgcatcg tcgccgcgtt gcatctcgtc gctcccgacg cctacctcag tcgcgcaccc
     2401 cctctcgttt cgctgtgacc gttgccgcca cgccggcccg cctcgatgcg gtcggcgtgc
     2461 tcagacgtca ttgacgaaag cgagacccca tgcacaagct cgtgccagcg acgttcgcgc
     2521 tggccctgat ccttccgttc ggcgcgcgcg ccgataccgc cgccgatgtc gccgccctgc
     2581 gccaggaaat ggacgcaatg cgcgccagct acgaggcgcg ccttgaatcg ctcgaagagc
     2641 gcctgcgacg gaccgaggcg gccttgcccg tcgccgccgc gagcgcgccg ccggctgcca
     2701 tggcggcgaa tgcgtcgccg gtcgtcgtcg cccgctcgtc cgctgcgccg cctgcggtcg
     2761 ccgccactgc cgcgctgcct gccgtcgccg cgaccgtcgt cccgccgccg cccgacaccg
     2821 gctccggcag cggcgggctc aacgccttca acccgtcgtt gtcgctgatt ttgtcgggcc
     2881 tctacgcgcg cacgtcgcag gacccgtcgc gctacgcgat caccggcttt cccctgccgc
     2941 cggacgccca gatcggtcct ggcacccgcg gcttcagcct cgacgagtcg gagctcggcg
     3001 tggctgccag catcgacccg tggctgcgtg gtgctgccaa catcgccttc gaggccgaca
     3061 actcggtatc gattgaggaa gccttcgtgc agacgacctc gctcggccac ggcgtctcgc
     3121 tcaaggccgg tcgcttcttt tccggcatcg gctacctcaa cccgcagcac tcccacacct
     3181 gggacttcgt cgacaacccg ctcgcctacc aggcgatgct cggcacccag tacgacgacg
     3241 acggcgtgca gctgatgtgg ttgccgccga tcgaccagta cgtcgagatc ggcgccgagc
     3301 tcggtcgcgg ccgcagctat cccggcaccg acaccggtcg caacggcgcc ggcatgaact
     3361 cgatcttcgc gcatacgggc ggcgacatcg gcgacagcca gagctggcgt gccggcgtct
     3421 cgatgctcga cgccaaggcg agtgcgcagc agcttcgcgc gaccgatacc ctcggccatg
     3481 tcgtcaccaa tgcgtttgac ggcagcacgc gcgtctggat cgccgacgcg atctggaagt
     3541 gggcgcctca cggcaacgcc acgcggacca acttcaagct gcagggcgaa tacctgcaca
     3601 gcacgcgcag cggcaacctc gcctacgaca tcgacggcat cgacagcgcc ggtgcctatc
     3661 gcgccgccca gtctggctgg tacgtgcagg gtgtctggca attcatgccg tactggcgcg
     3721 tcggcctgcg caccgagcgg ctcgaccccg gcacgcccga ctacggcctg aacagcgacg
     3781 tcctcgcgac gagcggcttt catccgcaca agaacacgct gatggtcgac ttcaacccga
     3841 gcgagttctc gagggtgcgt ctgcagttcg cccgcgatca atcgcgcgaa ggcgttgccg
     3901 acaacgaggt cttcctgcaa taccagatga gcctgggcgc gcatggcgcg cacagctact
     3961 gaccgacatt tcccggagcc cgcttccatg aatcctcgtt ttcattcgat cctgacgctg
     4021 ctcgcggcgc cgatcctctt cctggcgctg ccggcgcagg cggccctgca tgtcttcgcc
     4081 tgcgagcccg aatggggtgc gctggcccag gagctcggcg gcaagctcgt cgacgtctcg
     4141 gtggcgacca acgccctcca ggacccgcac cagatccagg ccaagcccag cctgatcgcg
     4201 cgtgcgcgca gcgcggacct cgtcgtatgc accggcgctg aactggaagt gggctggttg
     4261 ccggtgctgc tgcagcagtc gggcaatggc aaggtgcaac cggggcaacc
//
LOCUS       DASRWS010000546         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_508354, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000546 DASRWS010000000
VERSION     DASRWS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508354"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFF72_10330"
     CDS             <1..557
                     /locus_tag="VFF72_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZV93602.1"
                     /translation="AGALAVAQQVLIGLSIGFAAKLAVTAVEFAGELVGLQMGLNFAS
                     FFNPMSGGDETAVSRFYGVAVAWLFIVSGGHLVVIAAVTQSFTAFPVSSEPFAFLRAV
                     EPQRWGAEIFSTGLWIALPLIAMLLFTNLVLGVISRVAQQMNIFAIGFPVTLGVGLVG
                     MLLTLPMLQAPFTLALERMLARFQ"
     gene            691..1911
                     /locus_tag="VFF72_10335"
     CDS             691..1911
                     /locus_tag="VFF72_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZV93603.1"
                     /translation="MNAFRTLSSWIVTAVFLAACGGGDPDVPGSGSPSGAPTSKGNFT
                     AVVSFGDSLSDVGSYAPATSLAGNGQPPYFGGKFTTNETSNGAPDSNTLGKVWVEDLA
                     ATLGIVVTPAEVGFNGVSVKCPIAAAAPALAGTCTGYGQGGSRVTDPMGIGHNADGSG
                     ALTVPVVTQIANHLARFGSFKDSDLIFVYAGSNDVFTQFGTFTTAAAQIQAAAGAGTI
                     TADQANVQLFAAQQAAEAGMKQAAQDLANDIKTQILANGGKYVAVMTLSDIVDTPLGQ
                     SLPAAVQPVLTDLSQVFNLWLRDGLAGQPVQIIDTFALFKDAYQNPATYGFVNNTVPA
                     CDPDKISAITGGAVTDGSSLFCNATPGAPYNGLRDGADVTTWQFADDVHPTTGGHAVI
                     AAAFEAQLRAFGWI"
     gene            1941..2594
                     /locus_tag="VFF72_10340"
     CDS             1941..2594
                     /locus_tag="VFF72_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HZV93604.1"
                     /translation="MNHVPSLAAATLLALACNAAQADGIADGNNVLKLGITRYDTHSK
                     TNGISGIGVPPGADAEVGDATTAIFVYERLLTPQVGLEFVLGVPPRLKAKATGSVAFL
                     GDDVLSAKNVAPTLLLNYHFFDPSATWRPYVGAGINYTKFTDVRSSLAPDVQMGSSTG
                     LALQAGISYAITKDIGLFASIAKINVKSKVVASGPTVLTTTVDFRPLVYSAGVSFQF"
     gene            complement(2697..>3164)
                     /locus_tag="VFF72_10345"
     CDS             complement(2697..>3164)
                     /locus_tag="VFF72_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93605.1"
                     /translation="RYINDEKICVALAAMWAQLKVKVKVVTQPRATYFPKLEKYDFSM
                     YMLGWGGSVTDAETVITPVLRNHRDDGSGIGFYNYGRYKDDVFDALAAKTSVEPDPVK
                     REAMIKQALLRYKDQFATLPLHRQVIPWAARSNVDVVHRADNWLEVAWVKIGK"
BASE COUNT          488 a         1159 c         1015 g          502 t
ORIGIN      
        1 ccgccggcgc cctcgccgtc gcacagcagg tgctgatcgg cctgtcgatc ggcttcgcgg
       61 cgaaactcgc cgtcaccgcc gtcgagttcg cgggcgagct cgtcggcctg cagatgggcc
      121 tcaatttcgc ctccttcttc aacccgatga gcggcggcga cgagaccgcc gtcagccgct
      181 tctacggcgt cgcggtggct tggctgttca tcgtcagcgg cggccacctc gtcgtcatcg
      241 ccgcggtgac gcagagcttc accgcgtttc cggtgtcgag cgagccgttc gccttcctgc
      301 gcgcggtcga gccgcagcgc tggggcgccg agatcttctc gaccggcctg tggatcgccc
      361 tgccgctgat cgcgatgctg cttttcacga acctcgtgct gggcgtcatc tcgcgcgtcg
      421 cccagcagat gaacatcttc gcgatcggct ttccggtcac gctgggcgtc ggcctcgtcg
      481 gcatgctgct caccttgccg atgctgcagg cgccgttcac cctggcgctg gagcggatgc
      541 tggcccggtt ccagtagccc gcgacggtgg gccccggcca cagcgcgccg cggctcgcgg
      601 gtttcaccga ggtgtgcgac caagggatca ccctatagtg gccggcgaaa gcgaacgacc
      661 gttcgcaaac gcctgcacca cgaggtctgc atgaacgcat ttcgcaccct gagttcctgg
      721 atcgtcaccg ccgtgttcct cgcggcctgc ggcggcggcg accccgacgt cccgggcagc
      781 ggctcgccct cgggggcgcc gacgagcaag ggcaacttca ccgccgtcgt ctccttcggc
      841 gacagcctga gcgatgtcgg cagctacgcg ccggcgacct ctctggccgg caacggccag
      901 ccgccgtact tcggcggcaa gttcacgacc aacgagacga gcaacggcgc gcccgacagc
      961 aacaccctgg gcaaggtctg ggtcgaggac ctcgccgcca cgctcggcat cgtcgtgacg
     1021 ccggccgagg tcggcttcaa cggcgtgtcg gtcaagtgcc cgatcgcggc ggccgcgcct
     1081 gccctggccg gcacctgcac cggctacggc cagggcggct cgcgcgtcac cgacccgatg
     1141 ggcatcggcc acaacgccga cggctcgggc gcgctgaccg tgccggtggt gacgcagatc
     1201 gccaaccacc tggcccgatt cggcagcttc aaggattccg atctcatctt cgtctacgcc
     1261 ggcagcaacg acgtcttcac ccagttcggc acgttcacga cggccgccgc gcagatccag
     1321 gccgcggccg gcgccggcac gatcaccgcc gaccaggcca acgtgcagct cttcgccgcg
     1381 cagcaggccg ccgaggcggg aatgaagcag gccgcgcagg acctcgccaa cgacatcaag
     1441 acgcagatcc tcgccaacgg cggcaagtac gtcgccgtga tgacgctcag cgacatcgtc
     1501 gacacgccgc tcggacagtc gttgccggcc gccgttcagc cggtgctcac cgatctctcg
     1561 caggtgttca acctctggct tcgcgacggc ctggccggcc agccggtgca gatcatcgac
     1621 accttcgcgc tgttcaagga cgcgtaccag aacccggcca cctacggctt cgtcaacaac
     1681 acggtgccgg cctgcgaccc cgacaagatc agcgccatca ccggcggcgc ggtcaccgac
     1741 ggctcctcgc ttttttgcaa cgcgacgccg ggcgcgccct acaacggcct gcgcgacggc
     1801 gccgacgtga cgacctggca gttcgccgac gacgtgcacc cgacgaccgg cggccacgcg
     1861 gtcatcgctg ccgccttcga ggcgcagctc agggcgttcg gctggatctg accacagcaa
     1921 cgaggcgaag gaaccgcacc atgaaccacg tcccatcgct tgccgccgcc accttgctgg
     1981 cgctcgcctg caacgccgcg caggccgacg gcatcgccga cggaaacaac gtcctcaagc
     2041 tgggcatcac ccgctacgac acgcactcga agaccaacgg catcagcggc atcggcgtgc
     2101 cacccggcgc cgacgccgag gtcggcgacg cgacgacggc gatcttcgtc tacgagcggc
     2161 tgctgacgcc gcaggtgggg ctcgagttcg tcctcggcgt gccgccgcgc ctgaaggcca
     2221 aggccaccgg cagcgtcgcc ttcctgggcg acgacgtgct ctcggcgaag aacgtcgcgc
     2281 cgacgctgct tctcaactat cacttcttcg atccgtcggc gacctggcgg ccttacgtcg
     2341 gcgccggcat caactacacg aagttcaccg acgtccggtc gagcctcgcg cccgacgtgc
     2401 agatgggcag ctcgacgggg ctggcgctgc aggcgggcat cagctacgcg atcaccaagg
     2461 acatcggcct tttcgcgagc atcgccaaga tcaacgtcaa gagcaaggtc gtcgcctcgg
     2521 gcccgacggt gctgacgacg acggtcgact tccggccact cgtctactcg gccggcgtct
     2581 cgttccagtt ctgacggcag cgccggccgg cgcgaaacgg cagggcggca gcccgcgcct
     2641 gcattccggc gtccgccgtt ccagcctcat gggctcgacg cccgccgccc gcggcgctac
     2701 ttgccgatct tcacccaggc gacctcgagc cagttgtcgg cgcgatggac gacgtcgacg
     2761 ttgctgcgcg cggcccaggg aatcacctgg cgatgcagcg gcagggtcgc gaactggtcc
     2821 ttgtagcgca gcagggcctg cttgatcatc gcctcgcgct tgaccggatc gggctcgacg
     2881 ctcgtcttcg cggccagcgc gtcgaacacg tcgtccttgt agcggccgta gttgtagaag
     2941 ccgatgcccg agccgtcgtc gcggtggttg cgcagcaccg gcgtgatcac ggtctcggcg
     3001 tcggtgaccg agccgcccca gccgagcatg tacatgctga agtcgtactt ctcgagcttc
     3061 gggaagtagg tggcacgcgg ctgcgtgacg accttcacct tgaccttgag ctgggcccac
     3121 atcgccgcca gcgcgacaca gatcttctcg tcgttgatgt agcg
//
LOCUS       DASRWS010000547         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2493247, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000547 DASRWS010000000
VERSION     DASRWS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493247"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFF72_10350"
     CDS             complement(<1..1181)
                     /locus_tag="VFF72_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZV93606.1"
                     /translation="MPLPIFLPCAAGVEALLESEIRALLPAASATAQRGGVALAGDPR
                     EVMILNLESRLAQRVLVEVAAGDYRSEDDLYALARGVEWSDWITPRQTIRVDTTTQRS
                     PLRSLNFAALRVKDAVCDRMREASGERPSVDTARPDLPLVLHLGAEQAALYVDTSGEP
                     LFKRGWRADTGDAPLKETLAAAMLAAAGWHGSADAGGALHDPCCGSGTIAIEAAQRAC
                     GIAPGALRRFAFEKMLPFAGAEPRAELQRLRSRARERVHPPQVPIFASDLSFRMVDFA
                     RRNAERAGVAHAIVFNGGDALERPAPAIAADLPGTLMLNPPYGERIEVGGKAGAARSR
                     MRESAEDRATPGDFFARLAAHWKHAYTAHPAGWTAWILSPDMKLPGAMRLKESRRVPL
                     W"
     gene            complement(1198..2781)
                     /gene="rpoN"
                     /locus_tag="VFF72_10355"
     CDS             complement(1198..2781)
                     /gene="rpoN"
                     /locus_tag="VFF72_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZV93607.1"
                     /translation="MKASLQVRLSQHLALTPQLQQSIRLLQLSTLELHEEVEQMLEQN
                     PFLEAEEESAPPFEPLVERLTRSEQAGERESERAVDGGDGGDDAPAIDGAADFGGTER
                     EDWENGTEREDFDGIRETPGKTRTQNDADDFDDFDRDAPAQSLQDHLRGQLSSMRLAP
                     ADAAAVLVLIDSLDEDGLLADTLEDIAQRLRESGIEFEAQAAAGCADDEDVVEPLVAQ
                     LRCGLRFLQSMEPTGVGARDLAECLTLQLRQRPRGEAQAIAILVCKHHLPLLARRDMK
                     RLMAVTGADEPLLREAQALILGLEPKPARPFARAEANIVVPDVVVQKSGRGWKVVLNP
                     DVMPRLRINDLYASAIRQQRGAGRAAGGLNGAAAGLNSRLQEARWFVKNILQRFDTIQ
                     RVSQAIVERQKSFFTHGAIAMKPLVLREIADQLGLHESTISRVTTAKYMATPFGTFEL
                     KYFFGSSLATEAGGNASSTAVRALIGQIVGAEDASKPLSDSQISKMLEEQGIHVARRT
                     VAKYREALKIAPAQLRKAM"
     gene            complement(3068..>3660)
                     /gene="lptB"
                     /locus_tag="VFF72_10360"
     CDS             complement(3068..>3660)
                     /gene="lptB"
                     /locus_tag="VFF72_10360"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZV93608.1"
                     /translation="GLIRADGGEIAIEGQRVERMPIHLRSRLGLSYLPQEPSIFRKLN
                     VEENIRAVLELQVGADGKALKKEAIEEALEGLLKDLSIEKLRTSPAPALSGGERRRVE
                     IARALATRPRFILLDEPFAGIDPIAVIEIQRIISFLKRRGIGVLITDHNVRETLGICD
                     RAYIINEGRVLAEGTPAEIVGNADVRKVYLGENFRM"
BASE COUNT          508 a         1287 c         1306 g          559 t
ORIGIN      
        1 ttccacaagg gcacgcgccg cgattccttg agccgcatcg ccccgggcag cttcatgtcg
       61 gggctcagga tccaggcggt ccagccggcc ggatgcgcgg tgtaggcgtg tttccagtgc
      121 gccgcgaggc gggcgaagaa gtcgccggga gtcgcccggt cctcggccga ttcgcgcatt
      181 cgcgagcgcg ccgcaccggc cttgccgccg acctcgatcc gctcgccgta gggcgggttg
      241 agcatcagcg tgccgggcag gtcggcggcg atcgccggcg ccggccgctc gagcgcgtcg
      301 ccgccgttga agacgatcgc gtgcgcgacg ccggcccgct cggcattgcg ccgggcgaag
      361 tcgaccatcc ggaacgacag gtcgctggcg aagatcggca cctgcggcgg gtggacgcgt
      421 tcgcgggcgc ggctgcgcag tcgctgcagc tctgcgcgcg gctcggcgcc ggcgaaggga
      481 agcatcttct cgaaggcgaa ccggcgcagg gcgccgggcg cgatgccgca ggcgcgctgc
      541 gccgcctcga tggcgatcgt ccccgatccg cagcagggat cgtgcagggc accaccggca
      601 tcggccgagc catgccagcc ggcggcggcc agcatggccg cggccagcgt ctccttcagc
      661 ggcgcatcgc cggtgtcggc gcgccagccg cgcttgaaga ggggctcgcc ggaggtatcg
      721 acatagagtg ccgcctgctc ggcgcccagg tgcaggacga gcggcaggtc gggccgcgcg
      781 gtgtcgacgc ttggccgctc gccgctcgcc tcgcgcatcc ggtcgcagac agcatccttg
      841 acgcgcagcg ccgcgaaatt gaggctgcga agcgggctgc gctgcgtcgt cgtgtcgaca
      901 cgaatcgtct ggcgcggcgt gatccagtcc gaccactcga cgccgcgcgc cagcgcatag
      961 agatcgtcct cgctgcggta gtcgccggca gccacctcga ccaggacgcg ttgcgcgagg
     1021 cggctttcga ggttgaggat catcacctcg cgcggatcgc cggccagcgc cacgccgcca
     1081 cgctgcgccg tcgccgacgc cgccggaagc aaggcgcgga tctcgctttc gagcagcgcc
     1141 tcgacgcccg cggcgcacgg caggaagatc ggcaggggca tcgacgtgtc ggcctggcta
     1201 catcgccttg cgaagctggg ccggagcgat cttcagcgcc tcgcggtact tcgcgacggt
     1261 gcgccgggcc acatggatgc cctgctcttc gagcatcttc gagatctggc tgtccgacag
     1321 cggcttcgag gcatcttccg caccgacgat ctgcccgatc agggcccgca ccgcggtgct
     1381 cgaggcattg ccgcccgcct cggtggcgag cgaggagccg aagaaatact tgagctcgaa
     1441 ggtgccgaaa ggcgtcgcca tgtacttggc cgtcgtcacc cgcgagatgg tcgattcgtg
     1501 caggccgagc tggtcggcga tctcgcgcag cacgagtggc ttcatggcga tcgcgccgtg
     1561 cgtgaagaag ctcttctgcc gctcgacgat cgcttgcgag acgcgctgga tggtgtcgaa
     1621 tcgctgcagg atgttcttga cgaaccagcg cgcctcctga aggcgcgaat tcaggccggc
     1681 cgcggcgccg ttgagaccgc ccgcggcgcg gccggcgccg cgctgctggc ggatcgcgct
     1741 cgcatacagg tcgttgatgc gcagtcgcgg catgacgtcc ggattgagca ccaccttcca
     1801 gccgcggccc gacttctgca cgacgacgtc gggaacgacg atgttcgcct cggcccgcgc
     1861 gaacggtcgc gccggcttgg gctcgaggcc gaggatgagc gcctgggcct cgcgcagcag
     1921 cggttcgtcg gccccggtca cggccatcag ccgcttcatg tcgcgccgcg cgagcagcgg
     1981 caggtgatgt ttgcagacga ggatggcgat cgcctgcgcc tcgccgcgcg gacgctgccg
     2041 cagctgcagg gtcaggcact cggccagatc gcgcgcgccg acgccggtcg gctccatgct
     2101 ctgcaggaag cgaagcccgc agcgcagctg ggccacgagg ggctcgacga cgtcctcgtc
     2161 gtcggcacag ccggcagcag cctgcgcctc gaactcgatg ccgctctcgc ggaggcgctg
     2221 ggcgatgtcc tcgagcgtgt cggcgagcag gccgtcctcg tcgagcgaat cgatgagcac
     2281 gagcaccgcg gcggcatctg caggtgccag gcgcatgctc gagagctggc cgcgcaggtg
     2341 atcctgcagg ctttgcgccg gcgcgtcgcg atcgaaatcg tcgaaatcgt cggcgtcgtt
     2401 ctgggtgcgc gtcttgcccg gcgtctcgcg gattccgtcg aagtcctcgc gctcggtgcc
     2461 gttctcccag tcctcgcgct cggtgccgcc gaaatcggcc gcgccgtcga tcgccggggc
     2521 gtcgtcaccg ccatcgccgc cgtcgacggc ccgctcgctc tcgcgctcgc cggcctgttc
     2581 cgagcgcgtc agccgctcga ccagcggctc gaaaggcggc gccgactctt cctcggcctc
     2641 gagaaaaggg ttctgctcga gcatctgctc gacttcctcg tgcaactcga gcgtcgacag
     2701 ctggagcagc cggatcgact gctgcagctg cggcgtgagc gccagatgct gcgacaggcg
     2761 gacctgcagc gacgccttca tctcgctgcc tcgtcgcgca gccgacgtcc ccgtgagcgt
     2821 ggaggctccg gccggccttc gggccgcccc aaggccggcc gcgcccgctc gaggggcagc
     2881 gaacggacct gtgagcgcgg gggctccggc cggccgccgg gccgccccaa ggccggccgc
     2941 gccccctcgg ggggcagcga acggacccgt gagcttgggg gctccggccg gtcgccgggc
     3001 cgccccaagg ccggccgcgc cccctcgggg ggcagcgaac ggacccgtga gcgtgggggc
     3061 tccatcctca catccggaag ttctcgccca ggtagacctt gcggacgtcg gcgttgccga
     3121 cgatctccgc aggcgtgcct tcggcgagca cccggccctc gttgatgatg taggcgcggt
     3181 cgcagatgcc gagcgtctcg cgcacgttgt gatcggtgat gaggacgccg atgccgcgac
     3241 gcttcagaaa gctgatgatt cgctggatct cgatgaccgc gatcgggtcg attccggcga
     3301 acggttcgtc gagcaggatg aagcggggtc gcgtcgccag ggcgcgggcg atctcgaccc
     3361 ggcgccgctc tccgcccgag agagccggcg ccggcgaggt gcgcagcttc tcgatggaaa
     3421 gatccttcag caagccctct agcgcctcct cgatcgcttc tttcttcagc gccttgccgt
     3481 cggcacccac ctgcagctcc agaacggcgc ggatgttttc ctcgacattg agcttgcgaa
     3541 agatcgaggg ctcctgcggc aggtacgaca ggccgagccg cgatcgcaga tggatcggca
     3601 tgcgttcgac gcgctgtccc tcgatggcga tctcgccccc gtcggcgcgg atcaggccga
//
LOCUS       DASRWS010000548         5774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1925097, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000548 DASRWS010000000
VERSION     DASRWS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925097"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VFF72_10365"
     CDS             <1..125
                     /locus_tag="VFF72_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZV93609.1"
                     /translation="FRERFEAKGRFRDYLAAIPTRVIHDASMLALRGADRALDT"
     gene            complement(161..1579)
                     /locus_tag="VFF72_10370"
     CDS             complement(161..1579)
                     /locus_tag="VFF72_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV93610.1"
                     /translation="MTDASRSTDIPARLDRLPWGRFHTLVIAALGITWILDGLEVTLA
                     GSVSGALKSSAQLHLSDAQIGLSASLYLVGAVSGALVFGALADRFGRKRLFNVTLGLY
                     LVATALTATSNSFAAFAFFRALTGAGIGGEYAAINSAIQELIPARFRGRIDLLVNGSF
                     WIGAALGAGGAVLLLAPGRLPPDVGWRVAFGVGAALGLVILVLRRFIPESPRWLMTHG
                     RLDEAERVMAQIEATANAPAIDAPLPRLRLSGRRTGMADVARTLLRTYPRRSLFVLVL
                     MASQAFFYNAIFFTYALVLGRFFGVADDRIGLYILPFAAGNFLGPLLLGPLFDIVGRR
                     AMIGLTYAASGLLLAGTALLFVDARIGATGMTLAWSVVFFFASAAASSAYLTAGESFP
                     LEMRALAIALFYSLGTAFGGVVGPWLFAALIGSGERSQIGWGYAFGAFVMLAAAAATR
                     KLGVAAERRALEDVSPPLTSRG"
     gene            1718..3625
                     /gene="glgB"
                     /locus_tag="VFF72_10375"
     CDS             1718..3625
                     /gene="glgB"
                     /locus_tag="VFF72_10375"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZV93611.1"
                     /translation="MTIEEDVYLFREGTNARMYEQLGCHLVRGGARFAVWAPNARAVA
                     VIGDWNDWNGQAHPLRPRDDGSGIWEGEVAGVGRGHLYKFRVAGADGGSADKADPFAF
                     CTELPPATASRVWSLEHEWGDAAWMATRAAKNALDAPISIYEVHAGSWRRDGGRMLSW
                     HELATSLADYVLEMGFTHVELMPVTEHPFYGSWGYQTTGYFAPTARYGTPQDFMAFVD
                     HLHQRGIGVLLDWVPSHFPVDAHGLAQFDGTHLFEHADPRQGFHPEWSSAIFNYGRNE
                     VRSFLMSSGCFWLDKYHIDGLRVDAVASMLYLDYARKSGEWVPNVHGGKENLDAVELL
                     RRFNEAAYRDHPDTQTIAEESTAWPMVSRPTSMGGLGFGMKWNMGWMHDTLAYMSQDP
                     IHRRHHHGGLTFSLVYAFNENFVLPLSHDEVVHGKGSLLARMPGDEWRQFANLRALFG
                     LMWTHPGKKLLFMGGEFGQRREWTHEGELEWWVAGMPLHAGVRRWVADLNRLLRSEPA
                     LHELDFSADGFEWVESQDAGNSVVAFLRKPRRGEALLVVCNLTPVPREGYRVGVPVAG
                     YWRELLNSDASGYGGSGAGNLGGVASQNVSAQGREHSLALMLPALSTLVFKRDVSAEP
                     PRPGAVGAAKA"
     gene            3622..5616
                     /locus_tag="VFF72_10380"
     CDS             3622..5616
                     /locus_tag="VFF72_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HZV93612.1"
                     /translation="MTRIGRPDGRVRAVIDAVWPSIDGGRFAAKRIVGGDMIVEADCF
                     ADGHDALRVMLRWRAEGDERFAESEMTAEGNDVWRGSFPLRNVGRYGYTVAAWVDHFL
                     SWRLELERRVDAADIRIAVRVGAALIDEVATRAHGADRKALAAWAVQLRRDAADEAAD
                     VERLKDVALDADGARLAALYPDRGLQTIAAPELPLVVDRERAQFSAWYELFPRSASDE
                     PGRHGTFADVEARLPYVASLGFDVLYFPPIHPIGRVARKGRNNTLTVAAGDVGSPWAI
                     GAAEGGHTAILPELGTAEDFRRLVARAAQHGLEIALDIAFQCAPDHPWVKQHPSWFRM
                     RPDGSVQYAENPPKKYQDIYPFNFETEDWRELWGALKGVFEHWIGEGVTIFRVDNPHT
                     KSFAFWEWVVGELKRAHPGLIFLAEAFTRPKVMHRLAKLGYTQSYTYFTWRNTKHELV
                     EYFTELSRDRSREYFRPNAWPNTPDILHEQLHGGRRPMFMARLVLAATLAANYGIYGP
                     AYELLEGTPREPGSEEYRDSEKYQLRQWNLAGEGNLREFITRVNRIRRENPALHGDWS
                     LVFCPVDNDQLIAYAKKSGDNVVVCVVNLDPDHAQSGWVELDLAALGVEGASYQMHDL
                     LSDQHFFWEGPRNFVLLDPNKLPAHVMRLVPRGRSERDFG"
BASE COUNT          905 a         2007 c         2027 g          835 t
ORIGIN      
        1 cgtttcgcga gcgcttcgag gcgaagggac ggtttcgcga ctatctcgcg gcgatcccga
       61 cgcgcgtcat ccacgatgcg tcgatgctcg cgctgcgcgg cgccgaccgc gccctcgaca
      121 cctgatccgc ggctcggccc gacgcgcctt cggtcgtcgg tcagccccgg ctcgtcagcg
      181 gcggcgacac gtcctcgagg gcgcgccgct cggcggcgac gccgagcttt cgcgtcgccg
      241 ccgcggcggc cagcatcacg aaggcgccga acgcgtagcc ccagccgatc tggctgcgct
      301 cgccgctgcc gatgagcgcg gcgaagagcc acggaccgac gacgccgccg aatgcggtgc
      361 cgagcgagta gaagagggcg atcgccagcg cccgcatctc gagtggaaaa ctctccccgg
      421 cggtgaggta cgccgagctc gctgctgccg aggcgaagaa gaagacgacc gaccacgcca
      481 gcgtcattcc cgtcgcgccg atccgcgcgt cgacgaagag cagcgccgtg ccggcgagca
      541 gcaggccgga ggcagcgtag gtcaggccga tcatcgcccg tcggccgacg atgtcgaaga
      601 gcggcccgag gagcagcgga ccgaggaagt tgccggcggc gaagggcagg atgtacaggc
      661 cgatgcggtc gtcggcgacg ccgaagaagc ggccgaggac gagcgcgtag gtgaagaaga
      721 tggcgttgta gaagaaggcc tgcgaggcca tcagcacgag cacgaagagc gagcgtcgcg
      781 gataggttcg cagcagcgtg cgggcgacgt cggccatgcc ggtgcggcgg ccgctcaggc
      841 gcagccgcgg cagcggcgcg tcgatggccg gtgcgttcgc cgtggcttcg atctgcgcca
      901 tcacgcgctc ggcctcgtcg aggcggccgt gcgtcatcaa ccagcgcggg ctctcgggga
      961 tgaagcggcg cagcacgaga atgacgaggc cgagcgcggc gccgacgccg aaggcgacgc
     1021 gccagccgac gtcgggtggc agtcggcccg gcgccagcag cagcaccgcg ccgccggcgc
     1081 cgagcgccgc gccgatccag aagctgccgt tgacgagcag atcgatgcgg ccgcgaaagc
     1141 gcgccgggat cagctcctgg atcgccgagt tgatcgccgc gtactcgccg ccgatgccgg
     1201 cgccggtgag ggcgcgaaag aacgcgaacg cggcgaagct gttcgaggtc gccgtcagcg
     1261 cggtggcgac gagatacagg cccagcgtga cgttgaagag gcgcttcctg ccgaagcgat
     1321 cggcgagcgc gccgaagacg agcgcgccac tgacggcgcc gacgagatag aggctggccg
     1381 acaggccgat ctgcgcatcg ctcagatgca gttgcgcgct cgatttcagc gcccccgaga
     1441 ccgagccggc gagcgtcacc tcgagtccgt cgaggatcca tgtgatgccg agcgcggcga
     1501 tgacgagcgt gtggaagcgg ccccagggca ggcggtcgag ccgggccgga atgtcggtcg
     1561 atcggctggc gtcggtcatc gggctcgtcg tttgctctgg ggctggaatg cgcgcctgcg
     1621 ccgtgtcgtg aagggacggg gaggcatggt gcaacgataa ggtggcacga ggcacagggt
     1681 tgttcgcgtc gcgtgactgt gctacggtga accgagcatg acgattgaag aggatgtcta
     1741 tcttttccgc gagggcacga acgctcggat gtacgagcag ctcggttgcc acctcgtgcg
     1801 aggcggtgcg cgattcgcgg tctgggcgcc caacgcccgt gccgttgccg tgatcggcga
     1861 ctggaacgac tggaacggcc aggctcatcc tctgcgtccg cgcgacgacg gctcgggcat
     1921 ctgggaaggc gaggtcgcgg gggtcgggcg cggtcatctc tacaagttcc gcgtcgccgg
     1981 tgccgacggg ggcagcgccg acaaggccga ccccttcgcc ttctgcacgg agctgccgcc
     2041 tgcgaccgcg tcgcgggtct ggtcgctcga gcacgaatgg ggcgacgctg cgtggatggc
     2101 gacgcgcgcg gcgaagaacg cgctcgacgc accgatctcc atctacgagg tgcacgccgg
     2161 gtcgtggcgt cgcgacggcg gccgcatgct gtcgtggcac gagttggcca cttctctcgc
     2221 cgactacgtg ctcgagatgg ggttcacgca tgtcgagctg atgccggtca ccgagcaccc
     2281 gttctacggc tcgtggggct accagacgac cggctacttc gccccgacgg cccgctacgg
     2341 cacgccgcag gatttcatgg ccttcgtcga ccacctgcac cagcgcggca tcggcgtgct
     2401 cctcgactgg gtgccttcgc atttcccggt cgacgcgcac ggcctggcgc agttcgacgg
     2461 cacgcacctg ttcgagcacg ccgatccgcg ccagggcttt catccggagt ggagctcggc
     2521 gatcttcaac tacggccgca acgaggtgcg cagctttctc atgtcgtcgg gctgcttctg
     2581 gctcgacaag taccacatcg acggccttcg cgtcgatgcc gtcgcgtcga tgctctacct
     2641 cgactacgcg cgaaagagcg gcgagtgggt gccgaacgtt cacggcggca aggagaacct
     2701 cgacgccgtc gagctcctgc gccgcttcaa cgaggccgcc taccgcgacc atcccgacac
     2761 gcagacgatc gccgaggaat cgaccgcctg gccgatggtc tcgcggccga cctcgatggg
     2821 cggcctcggc ttcggcatga agtggaacat gggctggatg cacgacacgc tggcctacat
     2881 gagccaggat ccgatccatc gccggcatca ccacggcggc ctcaccttct cgctcgtcta
     2941 cgcgttcaac gagaacttcg tgctgcctct ctcgcacgac gaggtcgtgc acggcaaggg
     3001 gtcgttgctc gccaggatgc cgggcgacga gtggcgccag ttcgccaacc tgcgcgcgct
     3061 gttcggcctg atgtggacgc accccggcaa gaagctcctc ttcatgggcg gcgagttcgg
     3121 ccagcgccgc gagtggacgc acgagggcga gctcgaatgg tgggtcgccg ggatgccgct
     3181 gcacgccggc gtcaggcgct gggtcgccga cctgaaccgg ctgctgcgca gtgagccggc
     3241 gctgcacgag ctcgacttct ccgccgacgg cttcgagtgg gtcgagagcc aggatgccgg
     3301 caacagcgtc gtcgccttcc tgcgcaagcc gcgtcgcggc gaggcgctgc tcgtcgtctg
     3361 caacctgacg ccggtgccgc gcgagggcta ccgcgtcggc gttccggtcg ccggctactg
     3421 gcgcgagctg ctcaacagcg acgcttcggg ttacggcggc agcggtgccg gcaacctcgg
     3481 cggcgtcgcc tcgcagaacg tgtcggcgca gggccgcgag cactcgctgg ccttgatgct
     3541 gccggcgctc tcgacgctgg tcttcaagcg cgacgtgtct gccgagccgc ctcggccggg
     3601 cgccgtcggc gccgcgaagg catgacgcgc atcggccggc ccgacgggcg cgtgcgcgcg
     3661 gtcatcgacg cggtgtggcc ttcgatcgac ggcggccgct tcgccgccaa gcgcatcgtc
     3721 ggcggcgaca tgatcgtcga ggccgactgc ttcgccgatg gccacgacgc gttgcgcgtg
     3781 atgctgcgct ggcgcgccga aggcgacgag cgattcgccg agagcgagat gacggccgag
     3841 ggcaacgacg tctggcgcgg cagcttcccg ctgcgcaacg tcggccgcta cggctacacg
     3901 gtcgcggcgt gggtcgatca tttcctctcc tggcggctcg agctcgagcg ccgcgtcgac
     3961 gccgccgaca tccgcatcgc cgtgcgcgtc ggcgcggcgc tgatcgacga ggtcgcgacg
     4021 cgcgcccacg gcgccgatcg gaaggcgctt gccgcctggg ccgtgcagct gcgtcgcgac
     4081 gctgccgacg aggcggccga cgtcgagcgg ctgaaggacg tcgccctcga tgccgacggc
     4141 gcacgcctgg ccgccctcta tcccgaccgc ggcctgcaga cgatcgccgc gcccgagctg
     4201 ccgctcgtcg tcgatcgcga gcgggcgcag ttcagcgcct ggtacgagct gttcccgcgc
     4261 tccgcttccg acgagccggg tcggcacggc acgttcgccg acgtcgaggc gaggctgccc
     4321 tacgtcgcct cgctcggctt cgacgtgctg tacttcccgc cgatccatcc gatcggccgc
     4381 gtcgccagga agggccgcaa caacaccttg acggtggccg cgggcgacgt cggcagcccc
     4441 tgggcgatcg gcgccgccga aggcggccac accgcgatcc tgcccgagct cggcacggcg
     4501 gaagacttcc ggcgcctggt cgcgcgggcg gcgcagcacg gcctcgagat cgccctcgac
     4561 atcgccttcc agtgcgcgcc cgaccatccc tgggtcaagc agcacccgag ctggtttcgc
     4621 atgcggcccg acggcagcgt gcaatacgcc gagaacccgc cgaagaaata ccaggacatc
     4681 tatccgttca acttcgagac cgaggactgg cgcgagctgt ggggcgcgct gaagggcgtg
     4741 ttcgagcact ggatcggcga gggcgtgacg atctttcgcg tcgacaaccc gcacaccaag
     4801 tcgttcgcct tctgggagtg ggtcgtcggc gagctgaaac gcgctcaccc ggggctgatc
     4861 ttcctcgccg aggcgttcac ccggcccaag gtgatgcatc ggctcgccaa gctcggctac
     4921 acgcagtcgt acacctactt cacctggcgc aacaccaagc acgagctggt cgaatacttc
     4981 accgagctga gccgcgaccg cagccgcgag tacttccgtc ccaacgcctg gccgaacacg
     5041 cccgacatcc tgcacgagca gctgcacggc gggcgccggc cgatgttcat ggcccgcctc
     5101 gtcctcgccg cgacgctggc cgccaactac ggcatctacg ggccggccta tgagttgctc
     5161 gaaggcacgc cgcgcgagcc cggcagcgag gaatatcgcg actccgagaa ataccagctg
     5221 cgccagtgga acctcgccgg cgaaggcaac ctgcgcgagt tcatcacccg cgtcaaccgc
     5281 atccgtcgcg agaatccggc gctgcacggc gactggagcc tcgtcttctg cccggtcgac
     5341 aacgaccagc tcatcgccta cgcgaagaag agcggcgaca acgtcgtcgt ctgcgtcgtc
     5401 aacctcgacc ccgaccacgc ccagtcgggc tgggtcgagc tcgacctcgc ggcgctcggc
     5461 gtcgaaggcg cgtcgtacca gatgcacgac ctgctgagcg accagcattt cttctgggaa
     5521 gggccgcgca acttcgtcct gctcgatccg aacaagctgc cggcgcacgt gatgcgcctg
     5581 gtgccgcgcg ggcgcagcga gcgcgatttc ggctgaggaa tgaagccccc acgcccgttc
     5641 acgctcgctg ccccccgagg gggcgcccgc cgacggaccg gccaagccgg atccgcggcc
     5701 gcggctggcc gggtccccgt gcgcgggctc gcaacaacat gaacaagccc cttgccgaag
     5761 ccgagctcgc cgct
//
LOCUS       DASRWS010000549         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3671033, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000549 DASRWS010000000
VERSION     DASRWS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671033"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="aroC"
                     /locus_tag="VFF72_10385"
     CDS             <1..768
                     /gene="aroC"
                     /locus_tag="VFF72_10385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZV93613.1"
                     /translation="WQKYGIRDYRGGGRQSARETAVRVAAGAIARKWLRERYGVAIRG
                     HLAQLGPKAIPFEAWEHVDSNPFFAANASIVAELETFMDSLRKSGDSCGARVTVIATG
                     VPPGWGEPVYGKLDADLASAMMGINAVKGVEIGAGFRSVAQRGTTHGDELTPEGFASN
                     NAGGILGGISTGQDITVSIAIKPTSSIRSPRRSIDLEGRPTLVETHGRHDPCVGIRAA
                     PIAEAMLALVLMDHALRHRAQCAGVRVGSVPIPAGRG"
     gene            949..2160
                     /locus_tag="VFF72_10390"
     CDS             949..2160
                     /locus_tag="VFF72_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV93614.1"
                     /translation="MKNSPAGARRRLWTGGVLVLLVAAALGLALTQRAGSASAGNGAA
                     KSAEAVVPLEFTAAEVARPLVARMPLSIEFSGPLVAPRTAIVRAKASGTLLTLHVAEG
                     SRVRAGQVIGEIDLSDLQTRVSDRAALVESAQASLVEAERQHTANVGLAAQNFISATA
                     LQSSQARLDAARAQLKSAQAQLAVSRIGVREATLAAPIPGIVGKRYVLPGEKVSAEQQ
                     VVTVVDLSTLELAGSVGTHEVSLLQPGLPVEVRVEGQDDPVAGRIDRIAPAAEAGTRS
                     IGVVVVLANRGERFRAGQYAEARVKVADDTMRLTVPIGAVGQASGQDYVWTIEHGALV
                     RRIVITGRKDVPTGRVEVKKGLDREAQILAERFDNLREGAPARVVARRSGASASSVAG
                     RAHLPASATST"
     gene            2178..>2725
                     /locus_tag="VFF72_10395"
     CDS             2178..>2725
                     /locus_tag="VFF72_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZV93615.1"
                     /translation="MWFTRVSIRNPVFATMVMVALCVLGLFSYSQLRVERLPDITPPV
                     VFVSVAYPGASPDATESELTRPIELALNGIAGIKMIRSNSLEGRSETAVEFQVDADMN
                     RAVQDVRDKIASVQPAFPRDAKQPFVSRFDGDNAQPTVYLSLLGPDRSARELSLLADQ
                     VVRKRLERASGVARVDVGGLTVR"
BASE COUNT          397 a          966 c          964 g          398 t
ORIGIN      
        1 tggcaaaaat atggaatccg cgattaccgg ggcggcggca ggcagtcggc gcgcgagacg
       61 gcggtgcggg tcgcggcggg tgcgattgcc agaaaatggc tgcgcgagcg ttacggcgtc
      121 gctatccgcg gtcatctggc gcagctcgga cccaaagcga ttccattcga agcctgggag
      181 cacgtcgatt caaatccgtt ctttgccgcg aacgccagca tcgtcgcgga gctcgagacg
      241 ttcatggatt cgctgcgcaa gtccggcgac tcctgcggcg cacgcgtgac ggtgatcgcg
      301 acgggcgtac ctccaggctg gggcgagccc gtgtacggca agctcgatgc cgatctcgca
      361 tcggcgatga tggggatcaa cgcggtcaag ggcgtcgaga tcggtgccgg ctttcgctcc
      421 gtcgcgcagc gaggcacgac gcacggcgac gagctgactc cggaaggctt cgcgagcaac
      481 aacgccggcg gcatcctcgg cggcatctcg accgggcagg acatcaccgt ctcgatcgcc
      541 atcaagccga ccagctcgat ccgcagcccg cgccggtcga tcgatctcga aggccgtccg
      601 acgctcgtcg agacgcacgg tcgtcacgac ccctgcgtcg gcatccgcgc cgcgccgatc
      661 gccgaggcga tgctggccct ggtcctcatg gaccacgcct tgcgacatcg cgcccaatgc
      721 gccggagtga gggtcggctc ggtgccgata ccggccggcc gcggctgaac gcccgcctcg
      781 ccggggcggc gaggcgctgg cgttcagcct ctcgacgcgc cgcagaccgc cgcgcgacat
      841 gacgcgacgg cgaccgctcg tcgccgatgt tcgccgtcct atcccgtcga tccgcagccg
      901 gtcgcatgcg cggcggccga ggccgcgccc gcacctacac tcgcggccat gaaaaactcg
      961 cctgccggcg cgcggcgtcg cctctggacg ggtggcgtcc tggtgctgct cgtggccgcc
     1021 gcgctcggcc tggcgctgac ccagcgagcc ggcagcgcct cggccggcaa cggcgccgcg
     1081 aagtcggccg aggcggtggt gcccctcgag ttcactgcgg ccgaggtggc gagaccgctc
     1141 gtcgcccgca tgccgctctc gatcgaattc tccggtcccc tggtggcgcc gcggacggcg
     1201 atcgttcgcg ccaaggcgtc cggaacgctt ctcacgctgc acgtggccga aggcagccgg
     1261 gtccgcgccg gtcaggtcat cggcgagatc gacctctcgg atctgcagac ccgggtcagc
     1321 gatcgggccg cccttgtcga atcggcgcag gccagcctcg tcgaggccga gcgccagcac
     1381 accgccaacg tcggcctcgc ggcacagaac ttcatctcgg cgacggcgct gcagtcgtcg
     1441 caggcgcgtc tcgacgcggc gcgggcccag ctcaagtcgg cccaggccca gctcgccgtc
     1501 tcgcggatcg gcgtcaggga ggcgacgctg gcggcgccga ttcccggcat cgtcggcaag
     1561 cgctacgtgc tgccgggcga gaaggtgagc gccgagcagc aggtggtgac ggtggtcgac
     1621 ctgtcgacgc tcgagctggc cggctcggtc ggcacgcacg aggtctcgct gctgcagccg
     1681 ggcctgcccg tcgaggtgcg ggtcgaaggc caggacgatc ccgtcgccgg ccgcatcgat
     1741 cgcatcgcac ccgccgccga ggccggcacg cggtcgatcg gcgtggtcgt cgtccttgcc
     1801 aaccgcggcg agcgattccg cgccggccag tacgccgagg ccagggtcaa ggtcgccgac
     1861 gacacgatgc ggctgacggt gccgatcggc gccgtcggac aggcatcggg ccaggactac
     1921 gtctggacga tcgagcatgg agcgctcgtc cgccgcatcg tcatcaccgg tcgcaaggac
     1981 gtgccgacgg gccgggtgga agtgaagaag ggactcgacc gcgaggcgca gatcctggcc
     2041 gagcgattcg acaacctgcg cgagggcgcg ccggcacgtg tcgtcgctcg tcgaagcggt
     2101 gcgagcgcgt cgtcggtggc cggtcgcgcg catttgccgg cctcggccac gtcgacctga
     2161 cggcggagct ccgggccatg tggttcaccc gcgtctcgat ccgcaacccg gtgttcgcga
     2221 ccatggtcat ggttgcgctg tgcgtgctcg gcctgttctc gtacagccag ctgcgagtcg
     2281 agcggctgcc cgacatcacg ccgccggtcg tcttcgtcag cgtcgcctat ccgggcgcct
     2341 cgcccgacgc gaccgagagc gagctgacgc ggccgatcga gctggcgctc aacggcatcg
     2401 ccgggatcaa gatgatccgc tcgaacagcc tcgagggacg cagcgagacc gcggtcgagt
     2461 tccaggtcga cgccgacatg aatcgcgccg tgcaggacgt gcgcgacaag atcgcatcgg
     2521 tgcagccggc gtttccgcgc gacgcgaagc agcctttcgt ctcgcgcttc gacggcgaca
     2581 acgcccagcc gaccgtctat ctgtcgctgc tcggacccga ccgcagcgcc cgcgagctct
     2641 cgctgctcgc cgaccaggtg gttcgcaagc gcctggagcg ggccagcggc gtggctcgtg
     2701 tcgacgtcgg cggcctcacc gtccg
//
LOCUS       DASRWS010000550         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3097856, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000550 DASRWS010000000
VERSION     DASRWS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097856"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFF72_10400"
     CDS             complement(<1..383)
                     /locus_tag="VFF72_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HZV93616.1"
                     /translation="MKPFAFVFPGQGSQSVGMLDGWRDHPAVRQTLDEASTALGEDVA
                     RLIHEGPKEQLDLTTNTQPVMLTAGIACYRAWLAETQIAPVVVAGHSLGGVLALELAS
                     GKYDVEVAGVLALGVKVEWSQDDLGR"
     gene            complement(497..1486)
                     /locus_tag="VFF72_10405"
     CDS             complement(497..1486)
                     /locus_tag="VFF72_10405"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZV93617.1"
                     /translation="MMAAAARYSRISGTGSFLPPDRVSNQDLADRLAADGVETSDQWI
                     VERTGIRFRHFAAPSVTSSDLATEAARRALAAADCDPHDIDLIILATSTPDMVFPSAA
                     CMVQEKLGVHGCSAFDVQAVCSGFVYALTVADAMIRTGSATKALVIGAEVFSRILDFK
                     DRTTCVLFGDGAGAVVLEASETPGILASELHADGRHVGILCVPGTVAGGRVLGDPLLK
                     MDGPAVFKLAVSVLDSVARSVLAKAGRSEADIDWLIPHQANIRIMQGTAKKLKLDPHK
                     LIATVDRHGNTSAASIPLALDDAVRAGTIRRGDTVMLEGVGGGFTWGAALLDF"
     gene            complement(1483..2592)
                     /gene="plsX"
                     /locus_tag="VFF72_10410"
     CDS             complement(1483..2592)
                     /gene="plsX"
                     /locus_tag="VFF72_10410"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZV93618.1"
                     /translation="MAASSSPTRLAVDCMGGDHGPSVILPACRSFLVSHPDAELMLVG
                     RVDALRESEGWDRCLRVEASEVVEMTDSVEVALRRKKDSSLRVAVSQVKPVEGGMPLA
                     QACVSAGNTGALMAVSRYVLKTIDGIDRPAIATAMPNERDGFTTVLDLGANVDCSAEH
                     LLQFAVMGSALVAAMKGIESPSVGLLNIGEEAIKGGETIKRAGELLRAAGAAGLVNFQ
                     GNVEGNDIFTGTCDIVVCDGFVGNVALKTAEGLATMLASFARQEFKRNVLTKLAALAV
                     LPVLNRLRRRVDPRRYNGAALLGLRGLVFKSHGSADAFAFEQAMNRAYDAARNGLLDR
                     VQHRIQATLSAMPGAAGGEMPGAPPLPALAAAGSA"
     gene            complement(2651..2833)
                     /gene="rpmF"
                     /locus_tag="VFF72_10415"
     CDS             complement(2651..2833)
                     /gene="rpmF"
                     /locus_tag="VFF72_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZV93619.1"
                     /translation="MAVQQNKKSPSKRDMHRAHNALAKPGIAIEPTTGETHLRHHLSA
                     TGYYRGRKVLKTKSDA"
     gene            complement(2946..3500)
                     /locus_tag="VFF72_10420"
     CDS             complement(2946..3500)
                     /locus_tag="VFF72_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZV93620.1"
                     /translation="MTTKSGRFDPRHLDMRAFAAAHGEIAGDWPLAALARLAGTAIEG
                     SVASAAPVTWNAAAGRSVLAGAGSQAALSLRADANLAMQCQRCLQPVDVPLAVARRIF
                     FVEGEDAAAALDEDSDDDVLALEPAIDLRTLVEDELLLALPLIARHEECPQPLVAPLD
                     DLPQASGERPFAALAALKARRGDA"
     gene            3576..>4024
                     /locus_tag="VFF72_10425"
     CDS             3576..>4024
                     /locus_tag="VFF72_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZV93621.1"
                     /translation="MVARRLVLASTSPYRRALLERLRLPFDVRSPQVDETALSGESPH
                     DLVVRLALAKADAIAAKEASAVVIGSDQVADLDGAIIGKPGDHLRATAQLRAMRGKRV
                     VFRTGLAVVCREIGLREVDVADVAVRFRDFGDAEIERYLRLEQPYDCA"
BASE COUNT          633 a         1392 c         1392 g          607 t
ORIGIN      
        1 cggccgaggt cgtcctggct ccactcgacc ttcaccccga gcgccagcac cccggcgacc
       61 tcgacgtcgt acttcccgga cgcgagttcg agcgccagca cgccgcccag cgaatgcccg
      121 gcgacgacga ccggcgcgat ctgcgtctcg gccagccagg cgcggtaaca ggcgatgccg
      181 gcggtgagca tcacgggctg cgtgttcgtg gtgagatcga gctgctcctt cggcccctcg
      241 tggatgagcc gggcgacgtc ttcgccgagc gccgtcgacg cctcgtcgag cgtttgccgg
      301 accgcaggat ggtcgcgcca gccgtccagc atgccgaccg actgggagcc ctggccaggg
      361 aaaacgaacg cgaaaggctt catggcagcg aacggagtga gcgtgcgggc gccggcctcg
      421 cgtccgggcg ccccaagcgc ggccgtgccc cctcgggggg cagcgaacga agcgagcgtg
      481 ggggcaccat ttccctctag aaatcgagca gcgccgcacc ccaggtgaag ccaccgccga
      541 cgccttcgag catgaccgtg tcgccgcgcc ggatcgtccc ggcgcgcacg gcatcgtcga
      601 gggccaaggg gatcgacgcg gccgacgtgt tcccgtgccg gtcgacggtg gcgatcagct
      661 tgtgcgggtc gagcttcagc tttttcgcgg tgccctgcat gatgcggata ttggcctggt
      721 gcggaatcag ccagtcgatg tcggcctcgc tgcggccggc cttggcgagg acggaccgcg
      781 ccacgctgtc gagcaccgac accgccagct tgaaaaccgc cggtccgtcc attttcagga
      841 gcggatcgcc gaggacccgg ccgcccgcca ccgtgcccgg aacgcaaagg atgccgacat
      901 ggcgaccgtc ggcgtgcaac tcgctggcca gaatgccggg tgtctcgctc gcctcgagca
      961 cgacggcgcc ggcgccgtcg ccaaacagca cgcacgtcgt gcggtccttg aagtcgagga
     1021 tgcgcgagaa gacttcggcg ccgatgacga gcgccttcgt cgccgagccg gtacggatca
     1081 tcgcgtcggc gaccgtcaac gcgtagacga aaccggagca caccgcctgc acgtcgaacg
     1141 cagagcagcc gtgcacgccg agcttttcct gcaccatgca ggcggccgaa ggaaacacca
     1201 tgtcgggcgt cgacgtggcg aggatgatca gatcgatgtc gtgcgggtcg caatcggccg
     1261 cggccagggc ccgtcgcgcc gcttccgtgg cgagatcgct cgacgtcacc gaaggcgcgg
     1321 cgaagtgtcg aaagcggatg ccggtgcgct cgacgatcca ttggtcggag gtctcgacgc
     1381 cgtcggcggc aagacggtcg gccagatcct gattgctgac gcgatcgggc ggcaggaagc
     1441 tgccggttcc gctgatgcgc gaataacggg ccgcggcggc catcatgccg atccggccgc
     1501 tgccagggca ggcaacggcg gtgcgcccgg catttcgcca ccggccgcac ccggcatcgc
     1561 cgaaagcgtc gcctggatgc ggtgctgcac gcgatccagc aagccgttgc gggccgcatc
     1621 gtaggcgcgg ttcatcgcct gctcgaacgc gaaggcatcg gccgagccgt ggctcttgaa
     1681 gacgaggccg cgcagcccga gcaacgcggc gccgttgtag cggcgcgggt cgacgcgacg
     1741 ccggagccgg ttgagcacgg gcagcaccgc caacgctgcc agcttcgtca acacgtttct
     1801 cttgaattcc tgcctcgcga agctggccag catggtggcc aggccctcgg cggtcttcag
     1861 agcgacgttg ccgacgaaac cgtcgcagac gacgatgtcg caggtgccgg tgaagatgtc
     1921 gttgccttcc acgttgccct ggaagttgac gagcccggca gcgccggcag cgcgcagcag
     1981 ctcgccggca cgcttgatcg tctcgccccc tttgatcgcc tcttcgccaa tgttgaggag
     2041 gccgacgctc ggactctcga ttcccttcat ggccgcgacg agcgcgctgc ccatcaccgc
     2101 gaactgcagc aggtgctcgg ccgagcaatc gacattggcg ccgaggtcca gaacggtggt
     2161 gaagccgtcg cgctcattgg gcatcgccgt cgcgatcgcg ggccggtcga tgccgtcgat
     2221 ggtcttgagc acgtagcggg aaaccgccat gagggcgccc gtgttgcctg ccgagacgca
     2281 tgcctgggcc agcggcatgc cgccttcgac cggcttgacc tggctcacgg cgacgcgcag
     2341 cgatgaatcc ttcttgcgac gcagggcgac ttcgaccgaa tcggtcatct cgaccacttc
     2401 gctggcctcg acgcgcaggc atcggtccca gccttcggac tcgcgcaacg cgtcgacacg
     2461 cccgacgagc atcagctcgg cgtcgggatg actgacgagg aaggaccggc acgccggcag
     2521 gatgaccgac gggccgtggt cgccgcccat gcagtcgacg gcgaggcggg tcggggaaga
     2581 ggaagccgcc attgggcgag ggcgtgatcg gcaggagcct cgcgggcagc cggtctcacg
     2641 ccatgccgga tcaggcgtcg gacttggtct tgagcacctt gcggccgcgg tagtagccgg
     2701 tggcgctcag gtggtgacgc agatgcgttt cgcccgtggt gggctcgatt gcgatgcccg
     2761 gcttggcgag agcgttgtgg gcgcgatgca tgtcgcgctt cgacggcgac ttcttgttct
     2821 gctgaacagc catgtgattc tccaggggcc cgtgcggcac gagcggcgcg gcggcgatcg
     2881 atatcgtcga gccaccacga aaagccgaag attgtagcag ccgaggcctg cgcggccgcc
     2941 gaggctcagg catcgccccg cctggccttc agcgcggcca gtgccgcgaa cggacgctcc
     3001 ccggaagcct gcggcaggtc gtcaagcggc gccacgagcg gctgcgggca ttcttcatgc
     3061 cgcgcgatca gcggcagggc gagcaaaagc tcgtcctcga ccagcgttcg cagatcgatg
     3121 gccggctcca gggcgaggac atcgtcgtcg ctgtcttcat cgagcgccgc ggcggcgtct
     3181 tcgccctcga cgaagaagat ccgccgcgcc actgccagcg gcacatcgac cggctgcagg
     3241 caacgctggc actgcatggc gagattcgca tcggcacgca gcgacaaggc ggcctggctg
     3301 cccgcgccgg ccagcaccga gcgcccggcc gcggcattcc aggtcacggg cgccgccgag
     3361 gcgacacttc cttcgatggc cgtcccggcc aggcgtgcaa gcgccgccag cggccagtcg
     3421 ccggcgatct cgccgtgcgc ggcggcgaac gcgcgcatgt cgaggtggcg cggatcgaaa
     3481 cgccctgatt tcgtcgtcat cggccgtgac ctcgaatcgt catcggccga gtttagtggc
     3541 ggcgtgcccg gcctcggcga tgggacaatg aaaccatggt cgcccggcgc ctcgtgctcg
     3601 catcgacgtc gccctaccgg cgcgcgttgc tcgagcgcct ccgcctgccc ttcgacgtcc
     3661 gttcgccgca ggtcgacgaa accgcactct cgggcgaatc gccacacgat ctcgtcgtcc
     3721 ggctcgccct cgccaaggcc gacgcgatcg ccgcgaaaga ggcatcggcg gtggtcatcg
     3781 gctccgacca ggtcgccgac ctcgacggcg ccatcatcgg caagcccggc gatcacctcc
     3841 gcgccaccgc tcagctgcgg gcgatgcgcg gcaagcgcgt cgtctttcgc accggtcttg
     3901 ccgtggtctg cagggagatc ggccttcgcg aggtcgatgt cgccgacgtc gcggtccggt
     3961 ttcgcgactt cggcgacgcc gagatcgagc gctacctgcg cctcgagcag ccctacgact
     4021 gcgc
//
LOCUS       DASRWS010000551         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3960930, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000551 DASRWS010000000
VERSION     DASRWS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3960930"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /gene="gmhB"
                     /locus_tag="VFF72_10430"
     CDS             complement(<1..610)
                     /gene="gmhB"
                     /locus_tag="VFF72_10430"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZV93622.1"
                     /translation="MKLVILDRDGTINEDRDDFVKSPDEWVAVPGALEAIARLNHAGW
                     HTVVATNQSGLARGLFDMATLNAIHARMNRELTAVGGRIDAVFFCPHGPDDGCYCRKP
                     LPGLIEKIAERYGIDAGEVHVVGDSLRDLQAGAAAGCHTHLVRTGKGASLDDAQLATL
                     RRDLPGSQVHADLTAFAHALIQGERRERGIAADESDSLFGELG"
     gene            complement(621..2771)
                     /gene="glyS"
                     /locus_tag="VFF72_10435"
     CDS             complement(621..2771)
                     /gene="glyS"
                     /locus_tag="VFF72_10435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZV93623.1"
                     /translation="MTSTSSAALLVELFVEELPPKALKSLGAAFASALHAGLAGRDLI
                     DPAVATWEGFATPRRLGVLLPAVRALAPDRSMEIKLMPVAVGLAADGQATPALLKKLA
                     SLGAAASVVPALGRRGDGKGEALYYEATVCGATLAEGLQAALDEAIAGLPIPKVMQYQ
                     LADGWTTVGFVRPAHGLVALHGDQVVAVRALGLTAGRVTHGHRFEAKTPTISLRSAQS
                     YAGQLRDEGAVIASFAQRRAEIARQLEAVAAREAGLRPIADDALLDEVTGLVEKPNVL
                     ACEFEKEFLEVPAECLILTMKANQKYFPLLDAAGRLSHRFLVVSNIAPADPGRVIEGN
                     ERVVRPRLADARFFFDQDRKKRLDERVAALDRIVYHARLGTQGERVARVRAIAAAIVA
                     RLPLAGAATAVDRAALLAKADLTTDMVGEFPELQGVMGRYYALHDGESDEVAQAIEDH
                     YKPRFAGDELPRSTIGVVVALADKLETVAGLLGAGQVPSGDKDPFGLRRQALGIIRLL
                     VERRLALGLRDLVDAAIAAMPESARADPLAVAQFVLDRATGYFEDQGFDRKAIASVLV
                     PFGATSPLGELVEVVAEATRFVTSPAGQILVAANKRITNILRKSGFDIEFGVSPRGLP
                     QAPDSAVFAQDEERDLFAALMSSGEDALRLRKALRYAESLAALAPLSAPTQAFFDKVM
                     VNVDDSALRDNRIVLLQHARAYMNQVADLTLMAA"
     gene            complement(2771..3730)
                     /gene="glyQ"
                     /locus_tag="VFF72_10440"
     CDS             complement(2771..3730)
                     /gene="glyQ"
                     /locus_tag="VFF72_10440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZV93624.1"
                     /translation="MLSFQQIILRLQTYWSEQGCALLQPYDMEVGAGTSHTATFLRSL
                     GPEPWKAAYVQPSRRPKDGRYGQNPNRLQHYYQYQVVLKPAPADILDLYLGSLEVLGF
                     DLKTNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCRPITGEITY
                     GLERLAMYLQGVDNVYKLQWTEGLSYGDVYLQNEQEQSAYNFEHSDVEFLLTAFAAHE
                     RQAGALMGARLALPAYEQVLKAAHAFNLLDARGAISVTERAAYIGRIRNLARSVAQSY
                     LDSRARLGFPMAPPAWADEVKAQLDAKSAVVEKAAAKTAGKSA"
     gene            complement(3786..>4427)
                     /gene="lnt"
                     /locus_tag="VFF72_10445"
     CDS             complement(3786..>4427)
                     /gene="lnt"
                     /locus_tag="VFF72_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZV93625.1"
                     /translation="GLPLGNDEIGYTNSAAGISAATSHLPGGFYRYDKHHLVPFGEFI
                     PTGFHWFTRMMNIPLGDFNRGPVAAPSFVFRGERIAPNICYEDLFGEELAARFVVADE
                     APTILANISNIGWFGDTIAVPQHLEISRMRTLELQRPMIRATNTGATVVIDHRGTVTA
                     ALKPFTRGVLEASVEGRSGITPFAWWAGRFDLWPLAVLGAAVLVAAIAARRRP"
BASE COUNT          689 a         1477 c         1564 g          697 t
ORIGIN      
        1 cgccgagctc cccgaacagc gaatcggact cgtcggcggc gatgccacgc tcgcgccgct
       61 cgccctggat gagcgcgtgc gcgaaggcgg tcaggtcggc atgcacctgg ctgccgggca
      121 ggtcgcggcg aagggtcgcc agttgcgcgt cgtcgagcga cgcgcccttg ccggtgcgca
      181 cgagatgcgt gtggcagccg gcggcggcgc cggcctgcag gtcgcgcagc gaatcgccga
      241 cgacgtgcac ctcgccggcg tcgatgccgt agcgctcggc gatcttctcg atcaggccgg
      301 gcagcggctt gcggcagtag cagccgtcgt cgggcccgtg cggacagaag aagacggcgt
      361 cgatccggcc gcccaccgcg gtcagctcgc ggttcatgcg cgcatggatg gcgttgagcg
      421 tggccatgtc gaacaggccg cgggccaggc ccgactggtt ggtggcgacc acggtatgcc
      481 agcccgcgtg gttgagccgg gcgatcgctt ccagcgcccc gggcaccgcg acccactcgt
      541 cgggcgattt cacgaaatcg tcgcgatcct cgttgatcgt tccgtcgcgg tcgaggatga
      601 cgagcttcat cgcgatgtca tcaggccgcc atgagggtca gatcggcgac ctggttcatg
      661 taggcacggg catgctgcag caagacgatc cgattgtcgc gaagcgcgga gtcgtcgacg
      721 ttgaccatga ccttgtcgaa gaacgcctgc gtcggggcgc tgagcggcgc cagcgccgcc
      781 agcgattctg cgtagcgaag tgccttgcgc aggcgcagcg catcttcgcc cgacgacatg
      841 agcgccgcga acaggtcacg ctcttcgtct tgcgcgaata cggcggaatc gggcgcctgg
      901 ggcaagccac gcggcgaaac cccgaactcg atgtcgaacc cggatttgcg caggatgttg
      961 gtgattcgct tgttcgctgc gaccaggatt tgcccggccg ggctcgtgac gaagcgggtg
     1021 gcctcggcga cgacctcgac cagctcgccc agcggtgaag tggccccgaa cgggacgagc
     1081 accgaggcga tcgccttgcg atcgaatccc tggtcctcga agtatccggt tgcccggtcg
     1141 agaacgaact gggcgaccgc caatggatcg gcacgggcac tctccggcat cgccgcgatg
     1201 gccgcatcga cgagatcacg caagcccagc gcgagccgcc gctcgacgag caggcggatg
     1261 atgccgagcg cctgccggcg caatccgaac gggtccttgt cgccgctcgg cacctgaccg
     1321 gcgccgagca gcccggcgac ggtctcgagc ttgtcggcca gcgcgacgac gacgccaatg
     1381 gtcgagcgcg gcagctcgtc gccggcgaaa cggggcttgt agtgatcctc gatcgcctgc
     1441 gcgacctcgt cgctttcccc gtcgtgcagt gcgtaatagc gacccatcac gccctgcagc
     1501 tcggggaact cgccgaccat gtcggtcgtg agatcggcct tggcaagaag cgccgcacga
     1561 tcgaccgccg tggcggcgcc ggccagcggc aaccgcgcga cgatggcggc ggcgatcgct
     1621 cgaacgcgcg cgacgcgctc gccctgcgtg ccgagcctgg cgtgatagac gatgcggtcg
     1681 agtgcggcga cgcgctcgtc gagtcgcttc ttccggtcct ggtcgaagaa gaacctggcg
     1741 tcggccaggc gcggtcgcac cacgcgctcg ttgccttcga tgacgcggcc ggggtcggcc
     1801 ggcgcgatgt tgctgacgac gaggaagcgg tggctgaggc ggccggcggc gtcgagcagc
     1861 ggaaagtact tctggttggc cttcatcgtc aggatcaggc actcggccgg cacttcgaga
     1921 aattccttct cgaactcgca ggcaagaacg ttcggcttct cgacgaggcc ggtcacctcg
     1981 tcgagcaagg cgtcgtcggc gatcggtcgc aagccggctt cgcgcgcggc gacggcctcg
     2041 agctggcggg cgatctcggc gcggcgctgg gcaaagctcg cgatcacggc accttcgtcg
     2101 cgcagctgcc cggcatacga ctgcgccgat cgcaagctga tcgtcggcgt cttcgcctcg
     2161 aagcggtgac cgtgcgtgac ccggccggcg gtcagcccga gcgcccgcac cgcgacgacc
     2221 tggtcaccgt gcaacgccac gaggccgtgt gccggccgca cgaagccgac ggtggtccag
     2281 ccgtcggcga gctggtattg catgaccttg ggaatcggca agccggcgat cgcttcgtcg
     2341 agcgcggcct gcaggccttc ggcaagcgtc gcaccgcaaa cggtcgcctc gtaatagagg
     2401 gcttcgccct tgccgtcacc gcggcgcccg agtgcgggca ccaccgatgc ggcggcgccg
     2461 agcgaggcga gttttttcag cagcgccggc gtcgcctgtc catcggcggc aagaccgacc
     2521 gcgaccggca tcagcttgat ctccatcgag cggtcaggtg ccagggcgcg cacggcagga
     2581 aggagaacgc cgaggcggcg aggcgtggcg aagccttccc acgtcgcgac cgccggatcg
     2641 atcaggtcgc gcccggccag gccggcgtgc agcgcgctcg caaaggccgc gcccagcgac
     2701 ttcagggcct tgggcgggag ctcctcgacg aagagctcga cgagcagcgc cgcggacgag
     2761 gtcgacgtca tcacgcgctc tttccggccg tcttcgccgc ggctttctcg acgaccgcgc
     2821 tcttcgcgtc gagctgcgcc ttcacttcgt cggcccaggc cgggggcgcc atcggaaagc
     2881 cgagccgggc ccggctgtcg aggtagctct gggcgacgct gcgcgcgaga ttgcggatcc
     2941 tgccgatgta ggcggcgcgc tcggtgacgc tgatggcgcc gcgtgcgtcc aagaggttga
     3001 aggcgtgcgc cgccttcagc acctgctcgt aggccggcag cgcgagccgc gcgcccatca
     3061 gggcacccgc ctgcctctca tgcgcggcga acgccgtcag caggaattcg acatcgctgt
     3121 gctcgaagtt gtaggcgctt tgctcctgct cgttctgcag gtagacgtcg ccgtagctga
     3181 ggccttcggt ccattgcagc ttgtagacgt tgtcgacacc ctgcagatac atcgccaggc
     3241 gctccagccc gtaggtgatc tcgcccgtga tcggcctgca gtcgatgccg cccacctgct
     3301 ggaaataggt gaactgcgtc acctccatgc cgttgagcca gacttcccag cccaggcccc
     3361 aggcgcccag cgtcgggttc tcccagtcgt cctcgacaaa gcggacgtcg ttcgtcttca
     3421 ggtcgaagcc gagcacctcg agcgagccga gatagaggtc gaggatatcg gccggtgccg
     3481 gcttgagcac cacctggtac tggtagtagt gctgcaggcg gttcggattc tggccgtagc
     3541 ggccgtcctt gggccggcgg ctcggctgca cgtaggccgc cttccacggc tcggggccga
     3601 gcgagcgaag gaaggtcgcg gtgtggctgg tgccggcgcc gacctccatg tcgtacggtt
     3661 gcagcagcgc gcacccctgc tccgaccagt aggtctgcag gcgaaggatg atttgctgga
     3721 acgagagcat cgcggatctt ccggcaggcc gacccacagc gggcgcgctc cgaggagccg
     3781 tgattttagg ggcgccgcct tgccgcgatt gccgcgacca gcaccgccgc gccgagcacg
     3841 gccaggggcc agaggtcgaa ccggccggcc caccacgcga acggcgtgat gccgctacga
     3901 ccctcgacgc tggcctccag cacgccgcgc gtgaagggct tcagtgccgc ggtgaccgtg
     3961 ccgcgatgat cgatgacgac ggtggcgccg gtgttggtgg cgcgaatcat cggccgctgc
     4021 agctcgagcg tgcgcatgcg cgagatctcg agatgctgcg gcaccgcgat cgtgtcgccg
     4081 aaccagccga tgttgctgat gttggcgaga atggtcggcg cttcgtcggc gacgacgaag
     4141 cgtgccgcga gctcttcgcc gaacaggtcc tcgtagcaga tgttgggcgc gatgcgctcg
     4201 ccgcgaaaga cgaacgaagg tgccgccacc gggccgcggt tgaagtcgcc cagcgggatg
     4261 ttcatcatcc gggtgaacca gtgaaagccg gtcgggatga actcgccgaa cggcaccagg
     4321 tgatgcttgt cgtagcggta gaagccgccg ggcaggtgac tcgtggccgc cgagatgccg
     4381 gcggccgaat tcgtgtagcc gatctcgtcg ttgccgagtg gcaggcc
//
LOCUS       DASRWS010000552         5035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1092985, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000552 DASRWS010000000
VERSION     DASRWS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092985"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFF72_10450"
     CDS             complement(<1..618)
                     /locus_tag="VFF72_10450"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZV93626.1"
                     /translation="MTGSIRSRAAWVPDARAVVFALLTLFAFAGALPAHAQSQGQAPG
                     GDPPSRVARLSEASGNVWLLNPDDNEWIGIDRNRPLTSGDRIATDNGAHAEVTLGTTT
                     LRLGEATDLEIVQLDDTAYRLRLQSGSVALRVRGPLALAGFSLETGEGQFRVLALGHY
                     RFDRFDQSSNLSVYSGRAVFERRDTALVVAAGQQAQFWLDAAGAAQ"
     gene            899..1522
                     /locus_tag="VFF72_10455"
     CDS             899..1522
                     /locus_tag="VFF72_10455"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZV93627.1"
                     /translation="MLTDACPSKGPEKPAERIATLARLRRHLGVGRCLVLGLVFFALP
                     CRGEEPVVHVERHGKNVVIDVDMPVAVAPATAWAVLTDYDRMATFLSNLSSSRIVERR
                     GDVLHIAQSGRTQVAFMTFSFAAVRAVELVPMREIRSSLVSGDFESYSSTTRLVDLTG
                     GVRVIHHGEYVPKAWLPPMIGVAVIESETRKQFAEFAAEMLRRQAAQ"
     gene            1825..2016
                     /locus_tag="VFF72_10460"
     CDS             1825..2016
                     /locus_tag="VFF72_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93628.1"
                     /translation="MNRDHESAFQPDPVLAFALACRVERDRRACLDIAEEDRRFETQS
                     HERAVAEASAARAIAAAAR"
     gene            2140..2385
                     /locus_tag="VFF72_10465"
     CDS             2140..2385
                     /locus_tag="VFF72_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93629.1"
                     /translation="MTDLTRLSDQISELQGSLLAMECFINSLTEALPADTRPVVQAFY
                     ASESEAFRAALMASTAPEATVAAFERDVQRAQRLLDG"
     gene            complement(2503..4362)
                     /locus_tag="VFF72_10470"
     CDS             complement(2503..4362)
                     /locus_tag="VFF72_10470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZV93630.1"
                     /translation="MDMTRPSPLSKATDVPVAQVAKAALRRLAVERLEPTPENYERAY
                     RHEAGDSSGATALPARAHKLVERLALRALDGDAAGAAEVGHAIGTGRWEQAEHLLDMA
                     GDASSQALATLIERIVRGVERGGHNWTPARRKEGIHRVLAGSRSDSRRLQQRLAQLVN
                     SWEGDGAATDVDIDDAPADAVTQAAADAAIDRAAASPASSMPEPDVTATATTAPEATT
                     DAAKSNDWKRIALSLAAALRQALPMQDDANRELGSELARLADDLSAEGATALRADATE
                     ALVQRAERVLQHRHHLFEQLGKLCHELTSSLTDIAENDSWVKGQCEAMRLKIEEGLTA
                     RGVRQVSDMLHHARARHAEIKGEREKAREALKLMIQQMLAELGELGSKTGDFHQSVGR
                     YAEVIEEADSLESLTGVVREMVTESRAVQSLVQQTQARLQEEHSKATDLSERVVRLED
                     EMRRLSDEVSTDQLTQIANRRGLMQAFSIEQARLERNGGHLSIGLLDIDNFKRLNDEL
                     GHSAGDEALKSLAAIVSKTLRPTDRVARYGGEEFVVLLPETPVDEGEQILTRLQRSLT
                     GGLFMHKDKQVFVTFSAGVTGYRLSERIEIALERADQALYEAKRTGKNRTCIG"
     gene            complement(4376..>5035)
                     /locus_tag="VFF72_10475"
     CDS             complement(4376..>5035)
                     /locus_tag="VFF72_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZV93631.1"
                     /translation="LSEWAIRETARQARVWQDNFGFNDSIAVNLPSRLFERTDLVEYI
                     HNAVTTYGVPHHAIELEITETGLMKDLQNVIPSLHRLNEIGVEISIDDFGTGYSSLAY
                     LTTLPISELKIDRSFIRDLGMTPQSSAVVTAIIALARSLGLRVIAEGVENLRQMEVLH
                     RLGCGIMQGFLFSKAQPPEAIEEWLRQTVLPKKAPWIGRAGSTDGPEPPRATRARLSR
                     T"
BASE COUNT          769 a         1717 c         1693 g          856 t
ORIGIN      
        1 ctgcgcggca cctgccgcat cgagccagaa ctgcgcctgc tggccagctg cgacgaccaa
       61 cgcggtatcg cgcctctcga atacggcgcg cccgctgtag acgctgagat tgctcgactg
      121 atcgaaacgg tcgaagcggt agtgacccag ggcgagcacc cggaactggc cctcaccggt
      181 ttcgagcgaa aaccccgcaa gcgccagcgg cccgcgcacc cgcaacgcga cgcttccgct
      241 ttgcaggcgc agccgatacg cggtgtcatc gagttgcacg atctccaggt cggtggcttc
      301 gccgagacga agcgtcgttg tcccgagcgt gacctcggca tgcgcgccgt tgtccgtggc
      361 gattcgatcg ccgctcgtca ggggccggtt gcgatcgatg ccgatccact cgttgtcgtc
      421 cggattcaga agccagacgt tgccgctggc ctcgctcagc cgggcaacgc ggctcggcgg
      481 gtcaccaccg ggtgcctggc cctgcgactg tgcgtgcgcc ggcagagcgc cagcgaaggc
      541 gaacagggtc agaagcgcga agacgacagc gcgtgcatct ggaacccatg ccgcgcgact
      601 tcgaatcgat cctgtcatgg tgcgaactcc ccgaaagcgc gagaagatgc aacgggcatg
      661 ccctcggtaa tacgccaacg gtcgacgggg cactgcagtt gactgatagg cgtcaaccac
      721 atagcgctgc gcagaaaata cggttcattg cgatccaccg tatccgctgg cgacttaggc
      781 catccttaag aatgtggtct gccgcgggga gtttcgggac tctccggcgc tggtgcgatg
      841 agaacgtgac actgcgcgat cgttacgctt ggcccggccc ctctccaaga atcgtcacat
      901 gctcaccgac gcctgtccat cgaaggggcc ggaaaaaccc gccgagcgca tcgcgacgct
      961 ggcgcggctg cgtcggcacc tgggcgtcgg tcgttgtctg gtgctcggtt tggtcttctt
     1021 tgccctcccg tgccgtggcg aggagcctgt cgtccacgtc gaaaggcacg gcaagaacgt
     1081 cgtgatcgat gtggacatgc cggtcgcggt cgccccggcg acggcatggg cggtcctgac
     1141 agactacgac cggatggcga cgtttctcag caacctgagc tccagccgga tcgtcgagcg
     1201 ccggggcgat gtccttcaca tcgcgcagtc gggtcgcacc caggtcgcct tcatgacgtt
     1261 ctccttcgct gccgtgcgtg ccgtcgagct ggtacccatg cgagagatcc gctcctcgct
     1321 ggtctcgggc gacttcgagt cctattcctc gacgacccgg ctcgtcgacc ttaccggcgg
     1381 agtcagggtg atccaccatg gggaatacgt gccgaaggcg tggctgccgc cgatgatcgg
     1441 cgtcgccgtc atcgaatcgg agacccgcaa gcaattcgca gaatttgccg ccgagatgct
     1501 caggcgccag gcggcgcaat agtgacacgg cgcaccgagg cccggtctgt cactgaatcc
     1561 gctcgatccc ggcgcagcgt tgcaaagctc cctgacgaag cttctcttca cgacgcctgt
     1621 cgtggcaaag cgttcggcaa agtcgtccgc ttccatgtaa gactgtcggc ttcgccgcgg
     1681 actggccggc gacgacacgt cggttggcgg actcgacgag gcactcccca gcgacacccg
     1741 accgacaacg acccgcaggc ggtcctgtct ccgccatgac accttgcgag cacacaggca
     1801 cgcagtttgc gacgctttcc gcccatgaac cgcgaccatg aatctgcgtt ccagcccgac
     1861 ccggtcctcg cgttcgcgct ggcctgccgc gtcgaacgtg atcggcgggc ctgcctcgac
     1921 attgccgaag aagaccggcg tttcgagacg cagtcgcacg agcgcgccgt ggccgaggcg
     1981 tcggcggcgc gggcaatcgc cgccgcggcg cgctgagctc accgcgaatc cgtggccgac
     2041 ttgccggctc gaggtcgcca tgcaggctcc gtgctggggc cggcgatcct gggcgtcgac
     2101 gcgggccatg acgacgatcc acgtcttacg gagaacgaga tgacggacct gacgcgactc
     2161 agcgaccaga tcagcgagct gcagggcagc ctgctggcca tggagtgctt catcaactcg
     2221 ctgaccgaag cattgccggc ggatacccga ccggtcgtgc aggcgttcta tgcgagcgag
     2281 tccgaagcct tccgcgccgc gttgatggcg tcgacggcgc ccgaagcgac cgtggcggcg
     2341 ttcgagcgcg acgtgcagcg agcccaaaga ctcctcgacg gctgaccggc ttcccagcga
     2401 agcacgcgga ggagacgcga ccgagcgaga gacccacgga acgccgccgg cggccgaacg
     2461 tcgttgccgg ccagcgaaaa gccgctcgac aacggggtcg catcagccga tgcaggtccg
     2521 gttctttccg gttcgcttgg cttcgtagag cgcctggtcg gcgcgctcga gcgcgatctc
     2581 gatccgctcg gaaagacgat agcccgtcac gccggccgag aaggtgacga agacctgctt
     2641 gtccttgtgc atgaagaggc cgcccgtgag cgatcgctgc agccgcgtca ggatctgctc
     2701 gccttcgtcg accggcgtct cgggcaggag gacgacgaac tcctcgccgc cgtagcgggc
     2761 gacgcggtcg gtcgggcgca gcgtcttgct gacgatggcg gcgagcgact tcaaggcctc
     2821 gtcgccggcg ctgtgtccaa gctcgtcgtt caggcgcttg aagttgtcga tgtcgagcag
     2881 gccgatcgag agatggccgc cgttgcgctc gaggcgcgcc tgctcgatcg agaaggcctg
     2941 catcaggccg cgccggttgg cgatctgcgt cagctggtcg gtcgacacct cgtccgacag
     3001 gcgacgcatc tcgtcttcga gcctgaccac gcgctcggaa agatcggtcg ccttgctgtg
     3061 ctcttcctgc aggcgcgcct gcgtctgctg caccagcgac tgcacggcgc ggctctcggt
     3121 gaccatctcg cgcaccaccc cggtcaggct ctcgagcgag tcggcctcct cgatcacctc
     3181 ggcgtagcgg ccgacgctct ggtggaagtc gccggtcttc gagccgagct cgcccagctc
     3241 ggcgagcatc tgctggatca tcagcttgag cgcctcgcgc gccttctcgc gctcgccctt
     3301 gatctcggca tggcgggctc gcgcgtggtg cagcatgtcg ctcacctggc gcacgccccg
     3361 ggcggtgagg ccctcctcga tcttcaggcg catcgcctcg cactggccct tgacccagct
     3421 gtcgttctcg gcaatgtcgg tgaggctcga ggtcagctcg tgacaaagct tgccgagctg
     3481 ctcgaaaagg tggtggcggt gctgcagcac gcgctccgcg cgctgcacaa gcgcctcggt
     3541 cgcgtcggcg cgcagcgccg tggcgccttc ggccgacagg tcatcggcga gacgcgccag
     3601 ctcggacccg agctcgcgat tggcgtcgtc ctgcatcggc agcgcctggc gcaacgcggc
     3661 ggccaggctg agcgcgatgc gcttccagtc gttcgacttc gccgcatccg tggtggcttc
     3721 cggcgccgtc gtcgccgtcg cggtcacgtc cggctcgggc atcgatgacg ctggcgatgc
     3781 ggccgcgcga tcgatcgccg cgtccgcagc ggcctgcgtg acggcgtccg ccggtgcatc
     3841 gtcgatgtcg acatcggttg cggcgccgtc gccttcccag ctgttcacca gctgcgccag
     3901 gcgctgctgc agtcggcgcg aatcgctgcg gctgcccgca agcacgcggt ggatgccttc
     3961 cttgcgtcgc gccggcgtcc agttgtgacc gcctcgctcg acaccgcgaa cgatccgttc
     4021 gatcagcgtc gcgagcgctt gcgacgaggc gtcgccggcc atgtcgagaa ggtgctcggc
     4081 ctgctcccaa cggccggtac cgatggcgtg gccgacctcg gctgcgccgg ccgcgtcgcc
     4141 atcgagggcg cgcagggcca gccgctcgac gagcttgtgc gcgcgcgccg gcagcgccgt
     4201 cgcgccggac gaatcgccgg cctcatggcg gtaggcgcgc tcgtagttct ccggcgtcgg
     4261 ctcgaggcgc tccaccgcca gccgccgcaa cgccgccttc gcgacctgcg ccaccggcac
     4321 gtcggtcgct ttcgacaagg gcgatgggcg tgtcatgtcc atggcgactg ccggtctagg
     4381 tgcgggagag gcgggcgcgc gtcgcgcgcg gcggctcggg cccgtcggtg ctgccggccc
     4441 ggccgatcca gggcgccttc ttgggcagca ccgtctgccg cagccactcc tcgatcgcct
     4501 cgggcggctg ggccttgctg aagagaaagc cctgcatgat gccgcagccg aggcgatgca
     4561 ggacttccat ctggcgcagg ttctcgacgc cctcggcgat gacccgcagg ccgagcgatc
     4621 gtgccagggc gatgatggcc gtcaccaccg ccgagctttg cggcgtcatg ccgaggtcgc
     4681 ggatgaaact gcgatcgatc ttcagctcgc tgatcggcag cgtcgtcagg taggcgagcg
     4741 acgaatagcc ggtgccgaag tcgtcgatgg agatctcgac gccgatctcg ttcaggcgat
     4801 gcagcgacgg gatcacgttc tgcagatcct tcatcagccc cgtctcggtg atctcgagct
     4861 cgatcgcgtg gtgcggcacc ccgtaggtgg tgacggcgtt gtggatgtac tcgacgaggt
     4921 cggtgcgctc gaacaggcgg ctcggcaggt tgaccgcgat cgaatcgttg aagccgaagt
     4981 tgtcctgcca gacgcgcgcc tgccgcgcgg tctcgcggat cgcccattcg ctgag
//
LOCUS       DASRWS010000553         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_510451, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000553 DASRWS010000000
VERSION     DASRWS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510451"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1048
                     /locus_tag="VFF72_10480"
     CDS             47..1048
                     /locus_tag="VFF72_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV93632.1"
                     /translation="MTATLVPSLFRVARRAAVLLLAGVGVAAFAEGSSDWPTRPIHIV
                     IPYGAGSSPDVFARLIADKISPRLGQAVVIDNKPGAGGNNGTAAVAKAAGDGYTFLVS
                     TNGPLVYNTVLYKHLGYDPFTELRPVVMGGTQANVCAVRTDSGITSLGQLVAAMRANP
                     GKFNFASTGVGSLSQLGVELLKTKTDTYAVHIPYASSPLAILALLQGDVQFACVPAVS
                     VMPQVRSGKLRPLAVSTATRSPFTPDIPTMKEAGLPDVENTAWMAVMAPASTPTPIVE
                     RMNKEINDALKMPEVREKLASMYMQPGGGTSADLAKFMHKELVTMTPVIKRSGASID"
     gene            1120..3189
                     /locus_tag="VFF72_10485"
     CDS             1120..3189
                     /locus_tag="VFF72_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZV93633.1"
                     /translation="MLLCTVARSDADDAEVEAAFARLDDGRGIYFGSDAGVVGLHPLG
                     ATLLDAPALAFSVFADGVEVEPIDAVGEALLAHPSLAEFAARCGRATGRDPIAALRAF
                     LAAMPRIEESLLVGALAFDAHRLAGSASSAPPATGMRLGVLFFAPSLLRRDAAGRWQR
                     VSLHFVDGLLAEVASSRAIVRSRPSAASESVPTSDARDDASKTSARDDFAPGGYAEVV
                     ARAIAELDGPGLLSLTLSQSFRRGVDPATSPARAFAALRAANPAPASFFVNDGEGERL
                     FGASPDLQLVVRGREVTALPVCGTVARGRGPVGEAEALRELLDEAVDAASLAICSDVL
                     RNDLAPLCVPGTLRLLDRRRPMALATVVHTVDRLRGELRDGIDAWDAIVATAAPVMAT
                     GAPRRRALELIAAHEASPRGWYGGLVVEVGGDGSARVGTILRAAALRDGTVEVRTGGD
                     LVRGSDPVREEHESRIKAVSLWRALGLPVDARLQASIAGDAVEKPARSRSTSASTVRL
                     VDAGDPLGCAVADMLVGLGFGLDAGAGVTVLVGADALRCAAAQAEATGLVAIGDAAAL
                     VLRRAGFAASGERPAQGELVRVAPAAGSRLEAQVPFRVMRYGSIGVDTTRAPAASAWQ
                     AWLVDDADRPVALAHRSRRTVCLLFRPESLLSDAAAGQVLRVACDSVGETRHHRHAPR
                     SGDDAAR"
BASE COUNT          413 a         1216 c         1146 g          513 t
ORIGIN      
        1 tcgccggcga ggaacgcttc ttcgcagaga ccgagcccat cgaaccatga cagcaactct
       61 cgttccatcc ctctttcgcg tcgcgcgccg cgctgccgtc ctcctgctgg ccggcgtcgg
      121 cgtcgcggcc ttcgccgagg gcagctccga ctggccgacc cggccgatcc acatcgtcat
      181 cccttatggc gccggctcct cgcccgacgt cttcgcgcgg ctcatcgccg acaagatctc
      241 gccgcgcctc ggccaggcgg tcgtcatcga caacaagccg ggcgccggcg gcaacaacgg
      301 caccgcggcg gtcgccaagg cggcgggcga cggctacacc ttcctcgtca gcaccaacgg
      361 gccgctggtc tacaacaccg tgctctacaa gcacctcggc tacgacccgt tcaccgagct
      421 gcgcccggtg gtcatgggcg gaacgcaggc caacgtctgc gccgttcgca ccgattccgg
      481 catcacgagc ctcggccagc tcgtcgccgc gatgcgggcc aatccgggca agttcaattt
      541 cgcatcgacc ggcgtcggca gcctctcgca gctcggcgtc gagttgctga agacgaagac
      601 cgatacctac gccgtgcaca ttccctacgc gtcgtcgccg ctggcgatcc tggcgctgct
      661 ccagggcgac gtgcaattcg cctgcgtccc cgccgtctcg gtgatgccgc aggtgcgctc
      721 gggcaagctt cgtccgctcg ccgtctcgac ggcgactcgc agccccttca cccccgacat
      781 cccgacgatg aaggaagccg gtctcccgga cgtcgagaac actgcctgga tggcggtcat
      841 ggcgccggcg agcacaccga cgccgatcgt cgagcgcatg aacaaggaga tcaacgacgc
      901 cctgaagatg cccgaggtgc gcgagaagct ggcgagcatg tacatgcagc cgggcggcgg
      961 cacgagcgcg gacctggcga agttcatgca caaggagctc gtcacgatga cgccggtcat
     1021 caagcgcagc ggcgcttcga tcgactaggg tcctgcccgc gtcatgggcc cgcgcgagcc
     1081 gtctcttcct gggtgggcct agggcccgcc gggcgcgtcg tgttgctctg caccgtcgcg
     1141 cgcagcgacg ctgacgacgc cgaggtcgag gccgccttcg cccgcctcga cgatggccgc
     1201 ggcatctact tcggcagcga cgccggagtc gtcgggcttc atcccctcgg ggcgacgttg
     1261 ctcgacgcgc cggcgctggc cttctccgtc ttcgccgacg gcgtcgaggt cgagccgatc
     1321 gacgcggtcg gcgaggcctt gctcgcgcat ccgtcgctcg cggaattcgc agcgcgttgc
     1381 ggtcgtgcaa ccggccggga tcccatcgcc gccctgcgcg cgttcctcgc cgccatgccg
     1441 cgcatcgagg aatcgcttct cgtcggtgcg ctcgccttcg acgcgcatcg gctcgccgga
     1501 tcggcgtcgt cggcgccccc ggcgaccggc atgcgactgg gcgtcctctt cttcgcgcct
     1561 tcgcttctgc gtcgcgatgc ggcgggccgc tggcaacgcg tgtcgctgca tttcgtcgac
     1621 ggtcttcttg ccgaggtcgc ctcgagccgc gccatcgtgc gctcgcgccc ctcggcagcg
     1681 agcgaatcgg tgccgacgtc ggacgctcgt gacgatgcgt cgaagacgtc ggcgcgcgac
     1741 gactttgcgc cgggcggcta cgccgaggtc gtcgcgcggg cgatcgccga gctcgacggt
     1801 ccgggcctgc tgtcgctcac gctcagccag agctttcgcc gcggcgtcga tccggcgacg
     1861 tcgccggcac gcgcgttcgc ggccttgcgc gccgccaatc cggcgccggc gagcttcttc
     1921 gtcaacgatg gcgaaggcga gcgcctgttc ggtgcttcgc ccgatctgca actcgtcgtt
     1981 cgcggacgcg aagtcacggc gctgccggta tgcggcacgg tcgcgcgggg ccggggtccg
     2041 gtcggcgagg ccgaggcgct gcgcgaactg ctcgacgaag cggtcgatgc ggcgtcgctc
     2101 gcgatctgca gcgacgtgct gcgcaacgat ctggcgccgc tctgcgttcc gggcaccttg
     2161 cgcctgctcg accggcgccg gccgatggcg ctggcgacgg tcgtgcacac ggtcgatcgc
     2221 ctgcgcggcg agctgcgcga cggcatcgac gcctgggacg cgatcgtcgc cacggcggcg
     2281 ccggtgatgg cgaccggagc gccgcgccgt cgtgcgctcg agctcatcgc ggcacacgag
     2341 gcgagcccgc gcggctggta cggcggcctc gtcgtcgagg tcggcggcga cggctcggcc
     2401 cgtgtcggca cgatcctgcg cgccgccgcg ctacgcgacg gcaccgtcga ggtccgcacc
     2461 ggaggcgacc tcgtccgcgg ctccgatccg gtacgcgagg agcacgaatc gcggatcaag
     2521 gcggtgtcgc tgtggcgcgc cctcggcctg cccgtcgacg cgagactcca ggcatcgatc
     2581 gccggcgatg ccgtcgagaa gccggcgcgt tccaggtcga catcggcgtc gacggtccgt
     2641 ctcgtcgacg ccggcgatcc gctcggctgc gccgtcgccg acatgctggt cggtctcggc
     2701 ttcgggctcg atgccggtgc cggtgtcacg gtgctggtcg gcgccgacgc gctgcgctgc
     2761 gccgcggcgc aggccgaggc gacgggactt gtcgcgatcg gcgacgcggc agcgctggtt
     2821 cttcgccgcg ccggcttcgc ggcatcgggc gagcgacccg ctcagggcga gctcgtccgc
     2881 gtcgcgccgg cggccggatc gcgcctcgag gcgcaggtgc cttttcgcgt catgcgctac
     2941 ggctcgatcg gtgtcgatac cacgcgcgcg ccggcggcgt cggcctggca agcctggctc
     3001 gtcgacgatg cggaccgacc ggtcgccctt gcgcatcggt cgcggcgcac cgtctgcctt
     3061 ctctttcggc ccgaatcgtt gctcagcgac gctgccgccg gccaggtgct gcgcgtggcg
     3121 tgcgactccg tgggcgagac acgtcaccac cggcacgcgc cgcgctccgg ggacgacgcc
     3181 gcgcgatgag cgacgacccg tgggatctcg agcgattcgt cgaggcgcag gccacctcct
     3241 tcgcgcaggc gcacgccgag ctcgccgccg gcaggaagcg cagccact
//
LOCUS       DASRWS010000554         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3412666, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000554 DASRWS010000000
VERSION     DASRWS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3412666"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFF72_10490"
     CDS             complement(<1..476)
                     /locus_tag="VFF72_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93634.1"
                     /translation="MSLIRLAVASCLLLGAAIAAPAGAAVRLDELRVPPGFRVELLTD
                     QVPNARQMALGRFEGGKGVVYVGSMREGKVYAVEIDAGKARAVHTIASDLEIPSGVAW
                     RDGKLYVGALSRILRFDGIDGRLAHPPAPVVVTDKLPDDKHHGARFLAFGPDGKLYV"
     gene            complement(495..1568)
                     /locus_tag="VFF72_10495"
     CDS             complement(495..1568)
                     /locus_tag="VFF72_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HZV93635.1"
                     /translation="MTESLVLAHERLTAAVRAIVAAAGSADAEAEAVAANLVEANLRG
                     HDSHGVGMTPRYIDAVLEGGLTVNAHVAVRADSGPLLTLDGQAGYGQVIGAEAMALGI
                     ARAREHGVAVVGLANSHHLGRIGHWAEQCIAAGLVSIHFVNVLARPIVAPFGGRDARL
                     GTNPFCVGIPRAGGEPVVLDFATSRIAQGKTRVAYNQGLPVPPGTIIDDRGEPTTDPR
                     FTVVEPAGALLPFGEHKGAGLALVCELLGGALAGGHTGAAAGDGRRRVLNGMFSVIVD
                     PARMGTAANLASEMESFIAWACASPPMSGVARVQTPGEPERETRAKRLAEGIEVDVVT
                     WAEIVAAGAKVGLAADRVEALAR"
     gene            1705..2196
                     /locus_tag="VFF72_10500"
     CDS             1705..2196
                     /locus_tag="VFF72_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93636.1"
                     /translation="MILPVRVLSSALATCLALAAGSVAAETSKTSQIYLQRQADGSAV
                     LTDHPSANALTERVWKMDREDPIAARERAAEVRREADAVSLRVQRSIESQQRLASEVD
                     ALRLAAQQRQDVADDDSAVGGVIVPLGDGGFMHRPHLGRRHHWPGRHPHRSFRGSAWP
                     AGR"
     gene            complement(2165..>2769)
                     /locus_tag="VFF72_10505"
     CDS             complement(2165..>2769)
                     /locus_tag="VFF72_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HZV93637.1"
                     /translation="RERLVRGELETPKPGDPLFDGHAVHLVATPQQSLEAAATTARAA
                     GIEAHILGDEIEGESREVGKVHAALARQIARRGQPFAAPCVVLSGGETTVTVKPSTGA
                     PGRGGRATEFLLGCAIALQGEARVHVLAADTDGIDGSEDNAGAIVAPDTLARAASIGL
                     KPRDCLDRNDAYGFFSALGDLVVTGPTFTNVNDLRAMLIL"
BASE COUNT          403 a          961 c         1022 g          383 t
ORIGIN      
        1 acgtaaagct tgccgtcggg gccgaaggcg agaaagcggg cgccgtgatg cttgtcgtcg
       61 ggcagcttgt cggtcacgac gacgggcgcc ggcggatggg cgagccgacc gtcgatgccg
      121 tcgaacctga ggatgcgcga cagcgcgccg acgtagagct tgccgtcgcg ccaggcgacg
      181 cccgacggga tctcgaggtc gctggcgatc gtgtggacgg cgcgcgcctt gccggcatcg
      241 atctcgaccg cgtagacctt gccctcgcgc atgctgccga cgtagacgac gcccttgccg
      301 ccctcgaagc ggccgagcgc catctgccgg gcgttcggca cctgatcggt gagcagctcg
      361 acgcgaaatc cgggcggcac gcgcagttcg tcgagccgca ccgcggcgcc tgcgggcgcg
      421 gcgatcgcgg cgcccaggag cagacacgaa gcgacggcca gccggatcag agacatcggc
      481 aaagcgtagc agcgtcagcg cgcgagcgct tcgaccctgt ccgcggcaag gccgaccttg
      541 gcgccggcgg cgacgatctc ggcccaggtg acgacgtcga cctcgatgcc ttcggccaac
      601 cgcttcgcgc gcgtctcgcg ttcgggttcg cccggcgtct gaacacgcgc gacgccggac
      661 atcggcggcg acgcacacgc ccaggcgatg aacgactcca tctccgaggc gagattcgct
      721 gcggtgccca ttcgggccgg gtcgacgatg accgagaaca tgccgttgag gacgcggcgg
      781 cgcccgtcgc cggccgcagc gccggtgtgt ccgccagcca gcgcgccgcc gagcagctcg
      841 cagaccagcg ccaggccggc gcccttgtgc tcgccgaagg gcaggagcgc gcccgccggt
      901 tcgaccacgg tgaagcgtgg atcggtggtc ggctcgccgc ggtcgtcgat gatcgtgccg
      961 ggcggcaccg gcaggccctg gttgtaggcg acgcgcgtct tgccctgcgc gatccggctg
     1021 gtcgcgaaat cgaggacgac gggctcgccg cccgctcgcg ggatgccgac gcagaacgga
     1081 ttcgtcccca ggcgcgcgtc gcgtccgccg aagggcgcca cgatgggtcg cgccaggacg
     1141 ttgacgaaat ggatcgagac gaggccggcg gcgatgcact gctctgccca gtggccgatc
     1201 cggccgagat ggtgcgagtt ggccagaccg accaccgcga cgccgtgctc ccgcgcccgg
     1261 gcgatgccga gcgccatcgc ctcggcgccg atcacctgcc cgtagccggc ctgaccgtcg
     1321 agcgtcagca gcggcccgct gtcggcacgc acggcgacgt gcgcgttcac cgtcaatccc
     1381 ccttcgagaa cggcgtcgat gtagcgcggc gtcatgccca caccgtgtga atcgtggccg
     1441 cgcaagttgg cctcgacgag gttggcggca acggcctcgg cttcggcgtc ggccgagccg
     1501 gcagcggcga cgatcgcgcg aacggccgcg gtcagtcgtt cgtgtgcaag gacgagggat
     1561 tcggtcatga gcggaaccgg ctgacgcggg aacgacgagc gtaacaagcc ccgcaccgct
     1621 gtcgggtcgg cagccatccc gatgcgcgcg agcgcgaacg gtggcacatt cgtccgcgtg
     1681 ttctcgcgtc ctgccggagt gatcatgatc ctgcctgttc gcgtcctttc gtccgcgctc
     1741 gcgacctgcc tggcactcgc tgccggcagc gtggcggcgg aaacatccaa gacgtcgcag
     1801 atctacctgc agcggcaggc cgatggctct gcggtgctga ccgatcaccc ttcggcgaac
     1861 gcgctgaccg agcgcgtctg gaagatggat cgcgaggatc cgatcgcggc gcgcgaacgc
     1921 gccgccgaag tgcggcgcga agccgacgcg gtgagcctgc gcgtgcagcg cagcatcgag
     1981 tcgcagcagc gcctcgcgag cgaggtggat gcgttgcgcc tcgcggcgca gcagcgccag
     2041 gatgtggcgg acgacgacag cgcggtcggc ggcgtcatcg tgccgctggg tgacggcggc
     2101 ttcatgcacc gaccccacct cggccgccgc catcactggc ccggccgaca cccgcaccgg
     2161 tccttcagag gatcagcatg gcccgcaggt cgttgacgtt ggtgaaggtc gggccggtca
     2221 cgacgagatc tccgagcgcc gaaaagaagc cgtaggcgtc gttgcggtcg aggcagtcgc
     2281 gcggcttcag gccgatcgac gcggcccggg ccagcgtgtc gggagcgacg atggcgccgg
     2341 cgttgtcttc gctgccgtcg atgccgtcgg tgtcggcggc gagcacgtgg acgcgagcct
     2401 cgccttgcag ggcgatggcg cagccgagca ggaactcggt cgcccggccg ccgcggccgg
     2461 gtgcacccgt agagggcttc acggtcaccg tcgtctcgcc gcccgagagg acgacgcacg
     2521 gtgcggcgaa aggctggccg cggcgagcga tctgccgcgc cagcgccgca tgcaccttgc
     2581 cgacctcgcg cgattcgccc tcgatctcgt cgccgaggat gtgcgcttcg atgccggcgg
     2641 cgcgcgcggt ggtggcggcg gcctcgagcg attgctgcgg cgtggcgacg aggtgcaccg
     2701 cgtggccgtc gaacagcgga tcgcccggct tgggcgtctc cagttcgccg cggacgaggc
     2761 gctcgcgga
//
LOCUS       DASRWS010000555         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1928439, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000555 DASRWS010000000
VERSION     DASRWS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928439"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFF72_10510"
     CDS             <1..193
                     /locus_tag="VFF72_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZV93638.1"
                     /translation="RHIAPNELATLMPDLARHVGACRFYNCTHVHEPGCGVVAALQRG
                     EIAAARYRIYLEILAELQR"
     gene            complement(198..1187)
                     /locus_tag="VFF72_10515"
     CDS             complement(198..1187)
                     /locus_tag="VFF72_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HZV93639.1"
                     /translation="MSFFAVLFALLLEQVKPLPRGNVVHDALTGWMRWTARNFDAGRD
                     AHAWVVWCVTALVPSLLTWAAFFGLQRINGLLGLAFDVAVLYLTLGFRQFSHYFTDIR
                     AALERGDEFEARRLLGEWRHVDASELPRAEFLRHLIEHSLLAAHRHVFGVFFWFVVMS
                     TFGLGPAGAVLYRMAEFASRFWAYRSGDGAVAINTRLMALSMRLFRLLDHVPARLTAS
                     GFAVVGNFEEAVNCWRRDAGLWKYDNEGVILAAAAGAVGIRLGGGSAPGLTPDRAKTF
                     ESGALPDSADAQGSTPGTVPQIGHLRSVVGLVWRSVVLWMLLLALLSLANLIG"
     gene            complement(1255..1755)
                     /locus_tag="VFF72_10520"
     CDS             complement(1255..1755)
                     /locus_tag="VFF72_10520"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZV93640.1"
                     /translation="MLVALVLREALTVLLTRRTDHLHDHPSQISFPGGRAEPTDADAV
                     ATALREAQEEIGLASASVEVLGSLPTYTTGTGFIVTPVVGLVRPGFDLHADPFEVAEV
                     FEVPLAWLMDPAHHQRHAFESGGATREFFSIPWPGVDAEGRPRRYFIWGATAAMLRNL
                     YRFLSA"
     gene            complement(1935..2288)
                     /gene="rplS"
                     /locus_tag="VFF72_10525"
     CDS             complement(1935..2288)
                     /gene="rplS"
                     /locus_tag="VFF72_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZV93641.1"
                     /translation="MNLIQTLEHEEIARLASKIPDFAPGDTVIVSVKVVEGTRKRLQA
                     FEGVVIAKRNRSLNSSFIVRKVSSGEGVERTFQTYSPLIASIEVKRRGDVRRAKLYYL
                     RQRSGKSARIKEKLA"
     gene            complement(2377..3138)
                     /gene="trmD"
                     /locus_tag="VFF72_10530"
     CDS             complement(2377..3138)
                     /gene="trmD"
                     /locus_tag="VFF72_10530"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZV93642.1"
                     /translation="MRFDILTLFPELFAPHLIHGVTRRAFESRSVDVRLWQLRDFGDP
                     PHARVDDRPYGGGPGMVLLFEPLLRALDAIRADRGDASPVPLVNFSPAGEALRQHAVA
                     SLAQGAGAILLCARYEGVDQRFIDRHVDIELSLGDFVLSGGEIPALALLDAVARLQPG
                     VLGDARSHEQDSFSEGLLDCPHYSRPEKLDGPGGAVPDVLLSGHHAAIGRWRRDRSLE
                     LTQKRRPDLVEAARQAGALDDTDRATLARQSAAKL"
     gene            complement(3152..>3601)
                     /gene="rimM"
                     /locus_tag="VFF72_10535"
     CDS             complement(3152..>3601)
                     /gene="rimM"
                     /locus_tag="VFF72_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZV93643.1"
                     /translation="PSNDRPVAGVGALPTRLEIRAARPHGEAIVAAARGIDDRDGVDV
                     LRGARIFLPRADFPAAGPDEFYWADLVGMTVINRDGESLGEVIGLLDNGAQSLLRVEP
                     RAAAAAGPDTGAARATSTERLIPFVAAFIDEVDLAGRRIVVDWGLDY"
BASE COUNT          624 a         1208 c         1273 g          496 t
ORIGIN      
        1 gcgccacatc gcgccgaacg agctcgcgac gctgatgccc gatctggccc gtcatgtcgg
       61 cgcgtgccgc ttctacaact gcacgcacgt ccacgaaccg ggctgcggcg tcgtcgcggc
      121 gttgcagcga ggcgagatcg ccgcggcgcg ctaccggatc tacctcgaaa tcctcgccga
      181 gctgcaacgc tgagttgtca gcctatgagg ttggccagcg agagcagcgc cagcagcagc
      241 atccacaaca cgaccgagcg ccagaccaga ccgacgacgc tgcgcaagtg gccgatctgc
      301 ggcaccgtgc cgggggtcga tccctgcgca tcggccgagt cgggcaaggc gccggattcg
      361 aacgtcttgg cacgatcggg tgtcaggccc ggcgccgaac cgccgcccag ccggatgccg
      421 accgcgccgg ccgccgcggc aaggatcacg ccttcgttgt cgtacttcca gaggccagcg
      481 tcacggcgcc agcagttcac cgcctcctcg aagttgccga cgacggcgaa ccccgaagcg
      541 gtgaggcgcg ccggcacgtg atcgagcagg cgaaacagcc gcatcgacaa ggccatcagg
      601 cgggtgttga tcgctactgc gccgtcaccg ctgcgatagg cccagaagcg gcttgcgaac
      661 tcggccatcc gatagagcac ggctcctgcc ggcccgaggc cgaaggtcga catgacgacg
      721 aaccagaaga agacgccgaa gacgtggcgg tgcgcagcca ggagcgaatg ctcgatgagg
      781 tggcgcagga actcggcccg cggcaactcg ctcgcgtcga catggcgcca ttcacccagc
      841 aaccgtcgcg cctcgaattc gtcccctcgc tccagggcgg cccggatgtc ggtgaaatag
      901 tggctgaact ggcgaaagcc gagcgtcagg tagaggaccg cgacgtcgaa cgccaggccg
      961 agcaggccgt tgatgcgctg caggccgaag aatgccgccc aggtgaggag ggaaggcact
     1021 agcgccgtca cgcaccagac gacccaggcg tgggcgtcgc gaccggcgtc gaagtttcgt
     1081 gccgtccagc gcatccatcc ggtcaacgcg tcgtgaacga cgttgccgcg aggcaggggc
     1141 ttgacttgct cgagcaacag ggcgaacaag acggcgaaga aactcattcc gacccatgat
     1201 agcgacaggg tctgccgcgc cgtcgccgcc gccagtgcgg cgcgtcgcac cgcttcaggc
     1261 cgacaggaag cggtagaggt tgcgcagcat cgccgcggtt gcgccccaga tgaaatagcg
     1321 gcgcggccgg ccttcggcat cgaccccggg ccagggaatc gagaagaact cgcgcgtggc
     1381 gccgccggac tcgaaggcgt ggcgctggtg atgggccggg tccatcagcc aggccagcgg
     1441 cacctcgaat acctcggcaa cctcgaacgg gtcggcgtga aggtcgaagc cgggtcggac
     1501 cagcccgacc acgggcgtga cgatgaagcc ggtgccggtg gtgtaggtcg gcagcgagcc
     1561 gagcacctcg acgctggccg aagccaggcc gatctcctcc tgcgcctcgc gcagcgccgt
     1621 ggcgacggcg tcggcatcgg tgggctcggc gcggccgccg ggaaagctga tctggctcgg
     1681 atggtcgtgg aggtggtcgg tgcgtcgggt cagcagcacg gtcaacgcct cgcgcagcac
     1741 cagcgcgacc agcaccgacg cagccgcggg aggccgctcg acgaatcgcc gctcgaccga
     1801 gatctcgggc tgccacgccg gcggatggcc gaagcggcgg cgcagcgcgt cgggcgcgat
     1861 atggtcgccg gcgacggccg gcaggtgcga atcggaaccg aggaccggca gggcctcggg
     1921 atcgaaggtc agactcaggc gagcttctct ttgatgcggg ccgacttgcc ggagcgctgg
     1981 cgcaggtagt agagcttggc gcggcgcacg tcgccgcggc gcttcacctc gatcgaggcg
     2041 atcagcgggc tgtaggtctg gaacgtgcgc tcgacgccct cgccgctcga gaccttgcgg
     2101 acgatgaagc tcgaattgag gctgcgattg cgcttggcga tgacgacgcc ttcgaacgcc
     2161 tgaaggcgct tgcgcgttcc ctcgacgacc ttgacgctga cgatgaccgt gtcgccgggc
     2221 gcgaaatcgg gaatcttgct ggcgaggcgc gcgatttcct cgtgctcgag ggtctggatg
     2281 agattcacgg aagtgctcct ggcgatcgtg ccggcgactt gtcgtcttta tggggccgct
     2341 cgggccctgc gccgcagagg atcgaaaaac ccgaaattat agctttgctg cgctctgccg
     2401 cgccaaggtc gcgcgatcgg tgtcgtcgag cgcgcctgcc tgccgggcgg cttcgacgag
     2461 gtcgggccgc cttttctggg tgagctcgag ggagcggtcc cggcgccagc ggccgatggc
     2521 tgcatgatgg cccgagagaa ggacatccgg aacggcgccg cccggcccgt cgagcttttc
     2581 gggccgactg tagtggggac agtcgagcag accctcggaa aaactgtcct gctcgtgcga
     2641 gcgggcgtcg ccgagaacgc cgggctgcag tcgtgccacc gcatcgagca gcgccaacgc
     2701 cggaatctcg cctccggaca gcacgaagtc gcccaggctc aactcgatgt ccacgtgcct
     2761 gtcgatgaag cgctggtcga cgccttcgta gcgcgcgcac agcaggatcg cgcccgcgcc
     2821 ttgtgccagg ctggccaccg catgctggcg aagcgcctcg ccggcgggcg agaagttcac
     2881 gagcggcacc ggcgacgcat cgccccgatc ggcccggatg gcgtcgagcg cgcgaagcag
     2941 cggctcgaaa agcagaacca tgcccggacc cccgccgtac ggccggtcgt cgacgcgggc
     3001 atgcggcgga tcgccgaaat cgcgcaattg ccacaaacgc acatcgaccg agcgcgactc
     3061 gaacgcccgg cgtgtcacgc cgtggatcag gtgcggcgcg aagagttcgg ggaagagcgt
     3121 caggatgtcg aagcgcatcg ccggtcgatt ctcagtagtc caggccccag tcgacgacga
     3181 tgcgccggcc ggcgaggtcg acctcgtcga tgaacgccgc gacgaaggga atgaggcgct
     3241 ccgtcgacgt cgccctggcg gcgcctgtgt ccgggcccgc cgcagcggcg gcacgcggct
     3301 cgacgcgcaa gaggctctgc gccccgttgt cgagcaaacc gatcacctcg ccgagcgatt
     3361 ctccgtcgcg gttgatcacc gtcattccca ccaggtcggc ccaatagaac tcatcggggc
     3421 cggccgccgg aaaatcggca cggggaagga agatgcgggc gccgcgcagc acatcgacgc
     3481 cgtcgcgatc gtcgatgccg cgcgccgcgg cgacgatcgc ctcgccgtgc ggccgcgccg
     3541 cccggatctc gagccgtgtc ggcaaggcgc cgacgccggc gaccggcctg tcgttcgagg
     3601 g
//
LOCUS       DASRWS010000556         6397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3962271, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000556 DASRWS010000000
VERSION     DASRWS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3962271"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..673)
                     /locus_tag="VFF72_10540"
     CDS             complement(134..673)
                     /locus_tag="VFF72_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZV93644.1"
                     /translation="MMKAVVIALAVGALSACATQSPDVVRPYEAQRMSQVYDATVLSV
                     RPVTIQGQQSGIGAGAGAVTGAVAGSTIGGHRDSIVGGVLGAVVGGVIGNAVERGATQ
                     QNAVEILVQLRNGERRAIVQANGNEGWSVGEPVVLVTTDGRTRVTRAPQVYPAAPQGY
                     PPLPPPPVYPPSGPVPPRS"
     gene            complement(826..1383)
                     /locus_tag="VFF72_10545"
     CDS             complement(826..1383)
                     /locus_tag="VFF72_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZV93645.1"
                     /translation="MEVLQLDMSGRPQAWISAKEAAVLYASDGVAWTLGHPFHVLRGG
                     VQRRTGLQSRIEVHPIVAVRGAVPSRAWRRTPALSNPKLFARDRQVCAYCVGRFDLDD
                     LTREHIVPTSRGGGDTWMNCITACRSCNGHKGNRLPEEAHMSLHYLPYVPSLHEDMIL
                     RGRRILADQMEFLLASVPRSSRLHG"
     gene            complement(1460..2455)
                     /locus_tag="VFF72_10550"
     CDS             complement(1460..2455)
                     /locus_tag="VFF72_10550"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZV93646.1"
                     /translation="MKVAIVGLGAVGGLIAARLAAAGHEVSALARGRTLALVRERGLV
                     VESNGRRSVAAIAASDDAASLGRQDLVVIALKAPALAAVAPALAAMTDERTVLLPAMN
                     GVPWWFMPAAMPDSPPLESVDPGGRLLAALPIDCVLGCVVHLAASCPEPGHVQHTFGE
                     RLIIGEPRGGDSRRAEGLRAMLAEAGFEAEASGDVRREVWYKLWGNMTMNPVSALTGA
                     ASDAILDDPLVFDFMVRVMAEAAAVGARIGCAIDQSGAERMKLARKLGSFRTSMLQDA
                     DAGRPIELDALVAAVHEIGGRVGVATPNVGALLGLTRLMGRQRGLYPPPADAARP"
     gene            2665..3252
                     /locus_tag="VFF72_10555"
     CDS             2665..3252
                     /locus_tag="VFF72_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZV93647.1"
                     /translation="MKGRQTRAAIVDAALGLASQSGIEGLSIGALAELTGMSKSGVFA
                     HFGSREELQISVVREYHARFEEEIFRPALREPRGLPRLQAMFDRWLDRVSIEVDSGCI
                     YISGAVEFDDRPGPVRDALVAMVQTWRAALERAIGAAVSEGHLHADTDVAQLLFEIHG
                     LILALHHDARFLRHPGAHERARAGFERVVAHWAAS"
     gene            3272..5062
                     /locus_tag="VFF72_10560"
     CDS             3272..5062
                     /locus_tag="VFF72_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZV93648.1"
                     /translation="MPSYAPPLRDMQFVLHEVLDVTGTLKALPRYADIDAATIDAVIE
                     EGGKFASEVLLPLNPVGDTEGCTLDKATHEVKTPAGFKAAYAKYVEGGWPSLSCDPEY
                     GGQGLPHIVNQCLYEMLNSSNQAWTMYPGLSHGAYEAMVAHGSDEQKRLYLGKLTSGH
                     WTGTMCLTEPQCGTDLGLLRTKAEPQADGTYKLTGNKIFISAGEHDMAENIIHLVLAR
                     LPDSPAGSKGISLFVVPKWKVKADGSLGERNGIWCGGLEHKMGIHGNATAQIVLEDAE
                     GTLVGEPNKGLAAMFVMMNAARLGVGMQSLGLTEVAYQNAAAYAKERLQMRSLSGAKE
                     KDKPADPIIVHPDVRKMLLTARAYAEGGRALAMFTTLLLDKELASEDESVRKDSADLV
                     ALLTPIVKAFFTDNAWIATSHCMQVFGGHGYIHESGMEQFVRDARINMIYEGTNTIQS
                     LDLLGRKVLSDNGAKLKKFGKIVQDFVEEEGVDEAMQEFVNPLADLGDKVTKLTTEIG
                     MKAFKDADEVGAAAVDYLRVCGHLVYAYLWARMAKVALEKEAGGDPFYTAKLAVARFY
                     FAKLLPETAGLIRTARSGAASLLAMDAALF"
     gene            5116..5571
                     /locus_tag="VFF72_10565"
     CDS             5116..5571
                     /locus_tag="VFF72_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HZV93649.1"
                     /translation="MTSIRRTLALALPLLFASTLALAQSMTPAGLWKTVDDKTKKEKS
                     LVRIVETDGVFTGKVEKIIDPDAPKDAVCKDCTDERKDKPILGMTIIRGVKATDDKAV
                     FEGGNILDPNNGKVYEVKLTVIDNGARLDVRGYIGVPLLGRTQTWSRVQ"
     gene            5586..>6397
                     /locus_tag="VFF72_10570"
     CDS             5586..>6397
                     /locus_tag="VFF72_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZV93650.1"
                     /translation="MNRFNVRKVAVLGAGVMGAQIAAHLVNVKVPVVLFDLAAKEGPK
                     NGIVARAVENLKKLKPAPLGIAEDAAFIEPANYEDNLDRLLECDLVIEAIAERMDWKL
                     DLYRKVAPAIAPHAIVASNTSGLSITKLSGVLPEEIQPRFCGIHFFNPPRYMALVELI
                     ATPTTEPKILDDLEAFVTSALGKSVVRAKDDARYIEPANYEDDIAGLAECDLVIEAIA
                     ERMDWKHDLYKKVAPHLAPHAIFATNTSGLSITNLSEGFSDELKSRFCGVHF"
BASE COUNT         1092 a         2155 c         2199 g          951 t
ORIGIN      
        1 cgttgcgacg tcgctatccg gccgacgcct acgtcggcat cgagctcgag gtgaaccagc
       61 gtttcgtcga agccgggggc gccgcgtggg acaggatcag gctcgatctc gtcgacacgc
      121 tgggacaggt gcttcagctg cgcggcggca ccggtccgga cggcggatag acgggcggcg
      181 gcggcagcgg cggatagccc tgcggcgcgg ccggatagac ctgcggcgcg cgggtgacgc
      241 gggtgcggcc atcggtcgtc acgaggacga cgggctcgcc gaccgaccag ccttcgttgc
      301 cgttggcctg gacgatggcc cgtcgttcgc cgttgcgaag ctggaccagg atctcgaccg
      361 cgttttgctg cgtcgcgccg cgttccacgg cgttgccgat cacgccgccg acgacggcac
      421 cgaggacacc cccgacgatg gaatcgcgat ggccgccgat cgtcgagccg gcaacggcac
      481 ccgtcacggc accggcgccg gcgccgatgc cgctttgctg tccctggatc gtcacgggtc
      541 gaaccgacag gacggtcgcg tcatagacct gcgacatccg ctgcgcctcg tagggtcgaa
      601 cgacgtcggg actctgggtg gcacacgccg agagcgcgcc gaccgccagc gcgatcacaa
      661 cggctttcat catggttttc tcctgcaaga tgtatctccg ccggcagcgc agcgcatgcg
      721 ctcaagtgga ccacaacggc acgtgattcg ccttggcggc gcgcgaagac ccggcaaaga
      781 agtgtggcag ttgtcgtgcc ggcggcgccg gcgcgaaagg gatgctcagc cgtgcaggcg
      841 gctgctgcgc ggcacgctgg cgagcaggaa ctccatctgg tcggccagga tgcgacggcc
      901 gcgcaggatc atgtcctcgt gcaggctggg aacgtagggc aggtagtgca gcgacatgtg
      961 cgcttcctcg ggcaggcggt ttcccttgtg cccgttgcag gaccggcacg cggtgatgca
     1021 gttcatccag gtatcgccgc cgccacggga ggtgggcacg atgtgctcgc gggtgaggtc
     1081 gtcgagatcg aagcgaccga cgcagtaggc gcagacctgg cgatcgcggg cgaagagctt
     1141 cgggttgctg agagccggcg tgcgccgcca ggcgcggctg ggcacggcac cgcgcacggc
     1201 gacgatcggg tggacctcga tgcgcgactg cagccccgtg cgtcgctgga cgccgccccg
     1261 cagcacatgg aagggatggc cgagcgtcca cgcgacgcca tcgctggcat agagcacggc
     1321 cgcttccttg gccgagatcc aggcctgggg gcggccggac atgtcgagct gcaggacttc
     1381 cacaacccga aagcgtaatg catccgcgaa acgacgcgaa ggacatcgcg cggtgcggcc
     1441 gcgacgcgat gcgccggcgc tacggtcgcg cggcgtccgc cggcggtgga tagaggccgc
     1501 gctggcggcc catcaggcgc gtcaggccga gcagtgcgcc gacgttgggt gtcgccacgc
     1561 cgacgcgccc gccgatctcg tggaccgcgg cgaccagggc gtcgagctcg atcggccggc
     1621 cggcgtcggc atcctggagc atcgaggtgc gaaagctgcc gagcttgcgc gcgagcttca
     1681 tccgttcggc tcccgactgg tcgatggcgc agccgatgcg cgcaccgacg gccgccgcct
     1741 cggccatgac gcggaccatg aagtcgaaga ccagcggatc gtcgaggatg gcgtcgctgg
     1801 ccgcgccggt cagcgccgag accgggttca tggtcatgtt gccccagagc ttgtaccaga
     1861 cctctcggcg cacatcgccg ctcgcctccg cctcgaagcc ggcctcggcg agcatcgcgc
     1921 gcaagccttc ggcgcggcgc gagtcgccgc ctcgcggctc gccgatgatg aggcgctcgc
     1981 cgaaggtgtg ctgcacgtgg ccgggctcgg ggcacgatgc cgcgaggtga acgacgcagc
     2041 cgagcacgca gtcgatcggc agggcggcga gcaggcgtcc gccggggtcg acgctttcga
     2101 ggggcgggga atcgggcatc gccgccggca tgaaccacca gggcacgccg ttcatcgccg
     2161 gcagcaggac cgtgcgctcg tcggtcatcg cggcgagcgc cggcgcgacc gcggcgagcg
     2221 ccggcgcctt gagggcgatg acgacgagat cctggcggcc gagcgaggcg gcgtcgtcgc
     2281 tggcagcgat cgccgcgacc gagcgacgac cgttcgactc gacgaccagg ccacgttcgc
     2341 gaacgagggc gagcgttcgt ccacgggcga gcgccgaaac ctcgtgcccg gccgcggcca
     2401 ggcgggctgc gatcaggcct ccgacggcgc ccaggccgac gatcgcgacc ttcacgagcg
     2461 acccctcgag tgccgggcgg tctcttgcgg accggtatca atttgtccct ggctgcgcgc
     2521 tggcatcgaa tttctgcaaa atagcacggt cgttcgtttt ttcatgggtg tcttcttcgc
     2581 gatgtgtctg ccgtgaattc tctcgccatc gacacgctcg ccgacccgct ggcgggcgac
     2641 gcggcgaacg cgcccaagtc gctgatgaag ggccgccaga cgcgggctgc catcgtcgac
     2701 gcggcgctcg gcctggcgtc gcagagcggc atcgagggcc tgtcgatcgg cgccttggcc
     2761 gagttgaccg gcatgagcaa atcgggggtc ttcgcccatt tcggctcgcg cgaggaactg
     2821 cagatctcgg tcgttcgcga gtaccacgcg cgtttcgagg aagagatctt tcgtccggcg
     2881 ctgcgcgagc cgcgcggact gccgcgtctg caggcaatgt tcgaccggtg gctcgaccgg
     2941 gtgtcgatcg aggtcgattc gggctgcatc tacatcagcg gcgccgtcga gttcgacgac
     3001 cggcccggac cggttcgcga cgcgctcgtg gcgatggtgc agacctggcg cgccgcgctc
     3061 gagcgcgcca tcggggccgc ggtgagcgaa ggacatctgc acgccgacac cgacgtggcg
     3121 cagcttctgt tcgagatcca cgggctcatt ctggccctcc atcacgacgc ccgattcctg
     3181 cgtcatcccg gcgcccacga gcgtgcgcgt gccggcttcg agcgcgtcgt cgcccactgg
     3241 gccgcttcct gaacttcacg aggacatcga catgccttcc tatgcgccgc ccttgcgtga
     3301 catgcagttc gtcctgcacg aggtgctcga cgtcaccggc accctcaagg cgctgccgcg
     3361 ctacgccgac atcgacgccg cgaccatcga cgccgtgatc gaggaaggcg gcaagttcgc
     3421 gagcgaggtg ctgctgccgc tcaacccggt cggcgacacc gagggctgca ccctcgacaa
     3481 ggcgacccac gaggtgaaga cgccggccgg cttcaaggcc gcctacgcca agtacgtcga
     3541 gggtggatgg ccttcgctca gctgcgaccc cgagtacggc ggccaggggc tgccgcacat
     3601 cgtcaaccag tgcctgtacg agatgctcaa ttcgtcgaac caggcctgga cgatgtaccc
     3661 cggcctgtcg cacggcgcgt acgaggcgat ggtcgcgcac ggttccgacg agcagaagcg
     3721 cctgtacctc ggcaagctga cgagcggcca ctggaccggc accatgtgcc tgaccgagcc
     3781 gcaatgcgga accgacctcg gactcctgcg cacgaaagcc gagccgcagg ccgacggcac
     3841 gtacaagctc accggcaaca agatcttcat ctcggccggc gagcacgaca tggccgagaa
     3901 catcatccat ctcgtcctcg cccggctgcc cgattcgccg gcgggatcga agggcatctc
     3961 gctcttcgtc gtgccgaagt ggaaggtcaa ggccgatggc tcgctcggcg agcgcaacgg
     4021 catctggtgc ggcggcctcg agcacaagat gggcatccac ggcaacgcca cggcgcagat
     4081 cgtcctcgag gatgccgaag gcacgctcgt cggcgagccg aacaagggcc tggcggcgat
     4141 gttcgtgatg atgaacgcgg cccggctggg cgtcggcatg cagtcgctcg gcctcaccga
     4201 ggtggcctac cagaacgccg ccgcgtacgc gaaggagcgg ctgcagatgc gttcgctctc
     4261 cggcgccaag gaaaaggaca agccggccga cccgatcatc gtccaccccg atgtgcggaa
     4321 gatgctgctc accgcgcgtg cctacgccga aggcggccgg gcgctggcga tgttcacgac
     4381 gctgctcctc gacaaggagc tcgccagcga ggacgagagc gtgaggaagg actcggccga
     4441 cctcgtcgcc ctgctgacgc cgatcgtcaa ggccttcttc accgacaacg cctggatcgc
     4501 gacctcgcac tgcatgcagg tcttcggcgg ccacggctac atccacgagt cgggcatgga
     4561 gcagttcgtc cgcgacgcgc gcatcaacat gatctacgag ggcacgaaca cgatccagtc
     4621 gctcgacctg ctcggccgca aggtgctctc cgacaacggc gccaagctga agaagttcgg
     4681 caagatcgtg caggacttcg tcgaggagga gggcgtcgac gaggcgatgc aggagttcgt
     4741 caacccgctc gccgaccttg gcgacaaggt gaccaagctg acgaccgaga tcggcatgaa
     4801 ggcgttcaag gacgccgacg aggtcggcgc cgcggcggtc gactacctgc gcgtctgcgg
     4861 ccacctcgtc tacgcgtacc tctgggcgcg catggccaag gtggcgctcg agaaggaagc
     4921 gggtggcgat ccgttctaca ccgccaagct ggcggtggcg cgcttctact tcgccaagct
     4981 gctgccggag acggccggcc tcatccgcac ggcgcgcagc ggcgccgcgt cgctgctggc
     5041 gatggacgcg gcgctcttct gagccgcgct gagccgcgcg agccccgcat cccgacagga
     5101 acacggagaa gacccatgac atcgatccgc agaaccctcg ccctcgcctt gccgctcttg
     5161 ttcgcatcga cgctggcgct ggcgcagtcg atgacgccgg ccggtctgtg gaagaccgtc
     5221 gacgacaaga cgaaaaagga gaaatcgctg gtccggatcg tcgagaccga cggcgtgttc
     5281 accggcaagg tcgagaagat catcgatccc gatgcgccga aagacgcggt ctgcaaggac
     5341 tgcaccgacg agcgcaagga caagccgatc ctcggcatga ccatcatccg cggcgtcaag
     5401 gcgaccgacg acaaggccgt cttcgaaggc ggcaacatcc tcgatccgaa caacggcaag
     5461 gtctacgagg tcaagctcac ggtgatcgac aacggcgcca ggctcgacgt gcgcggctac
     5521 atcggcgtgc cgctgctcgg tcgcacgcag acgtggtcgc gcgtgcaatg acatcaggag
     5581 cgagcatgaa tcgattcaac gtccgcaagg tcgccgtcct cggcgccggc gtcatgggtg
     5641 cgcagatcgc ggcccacctc gtcaacgtca aggtgccggt ggtgctgttc gacctggcgg
     5701 ccaaggaggg ccccaagaac ggcatcgtcg ccagggcggt cgagaacctg aagaagctca
     5761 agcccgcgcc gctcggcatc gccgaggacg ccgccttcat cgagccggcg aactacgagg
     5821 acaacctcga ccggctcctc gagtgcgacc tcgtcatcga ggcgatcgcc gagcggatgg
     5881 actggaagct cgacctctat cgcaaggtcg cgccggcgat cgcgccgcac gcgatcgtcg
     5941 cgagcaacac gtccggtctc agcatcacca agctctccgg tgtgctgccg gaagagatcc
     6001 agccgcgctt ttgcggcatc cacttcttca acccgccgcg ctacatggca ctcgtcgagc
     6061 tgatcgcgac gccgacgacc gagccgaaga tcctcgacga cctcgaggcg ttcgtgacga
     6121 gcgcgctcgg caagtcggtc gtccgcgcca aggacgacgc gcgctacatc gagccggcga
     6181 actacgagga cgacatcgcg gggctcgccg agtgcgacct cgtcatcgag gcgatcgccg
     6241 agcggatgga ctggaagcac gatctgtaca agaaggttgc gccgcatctc gcgccgcatg
     6301 cgatcttcgc gacgaacacg tcgggcctgt cgatcactaa cttgtccgaa ggattctcgg
     6361 acgagctgaa gtcgcgcttt tgcggcgtgc acttctt
//
LOCUS       DASRWS010000557         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2237058, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000557 DASRWS010000000
VERSION     DASRWS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237058"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFF72_10575"
     CDS             complement(<1..1115)
                     /locus_tag="VFF72_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HZV93651.1"
                     /translation="MSRLFEPWRIGALPLANRIVVAPMCQYSASDGTPGDWHLIHLGH
                     LALSGAGLLVVEATAVSAEGRITAGDLGLYSDANEQALGRVVAAIRAHSPIRVALQLA
                     HAGRKASSRAPWDGGAQIRLGEPGAWRAVAPSAVPHSPGEDAPQALGDAGMRKVRDDF
                     VAAARRAARLGFDGVELHAAHGYLLHQFLSPVANRREDEYGGSLENRMRFPLEVFDAV
                     RAVFPAQSPVWVRVSATDWVDGGWDVESTVAFGKALAARGCAAIHVSSGGVASTQKIP
                     VGPGYQVPLAARIKAEVGLPTIAVGLITDPEQAETIVASGQADAVALARGMLYDPRWP
                     WHAAARLGAHVAAPHQYWRSQPREFKDLFDNATFGAR"
     gene            complement(1184..1771)
                     /locus_tag="VFF72_10580"
     CDS             complement(1184..1771)
                     /locus_tag="VFF72_10580"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HZV93652.1"
                     /translation="MPARLTPPREPKSRPPISGDAERIAVVVMGVSGSGKTTIAEALA
                     SALGLHFIDGDSLHSPESVAKMRAAIPLQDDDRWPWLDRIGACLGDAQRWPQGVVVAC
                     SALKRTYRDRIRAAVPGLRFVFLDGSETLIAGRLAHRHAHYMPAALLASQLRTLEPPG
                     DDETDVLRLAIDRPVGEIVDDAARRLSAGPSPARS"
     gene            1957..2472
                     /locus_tag="VFF72_10585"
     CDS             1957..2472
                     /locus_tag="VFF72_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HZV93653.1"
                     /translation="MQTSRFAATVLAASLAAANVAWAQGDTQNVERADPHIQQPREPS
                     RTNSRDRGQSQMAQRHDNRGNSNGWNRGNDRQQGRSYDRDGPHDGYVRNDRSDHRNWG
                     AGPDHNFYRGGYLPREYRSRQYVVDDWRGHHLRPPPRGYHWVQTGGDYVLAAIATGVI
                     TSIILNAAANN"
     gene            2554..2949
                     /locus_tag="VFF72_10590"
     CDS             2554..2949
                     /locus_tag="VFF72_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93654.1"
                     /translation="MNRRTAGRASLPASWAAVALATAMLAGCGAPQARPDIQTVVTAR
                     VAGMSVGDFFDTFGRPRSRSERGDGSTDYLWISETKALGSGAANVDLSRCTLRLVADS
                     RNRIASVEVVLDSPGYTSTSRCNEIFAKK"
     gene            3031..>3283
                     /locus_tag="VFF72_10595"
     CDS             3031..>3283
                     /locus_tag="VFF72_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93655.1"
                     /translation="MAGGTVAGSAENPQNLAFRASTLASAMNDDPQERARQQAVDALG
                     LDSLTDTAYDDITRAAAELFGTPIALITILDGDRQWFKSR"
BASE COUNT          526 a         1168 c         1135 g          454 t
ORIGIN      
        1 cagcgggcgc cgaaggtcgc gttgtcgaag aggtccttga actcgcgcgg ctgcgagcgc
       61 cagtactgat gcggcgcggc gacgtgcgcg cccaagcgcg cggccgcgtg ccacggccag
      121 cgcgggtcgt agagcatccc ccgcgccagg gcgacggcat cggcctggcc gctggcgacg
      181 atggtctcgg cctgctccgg atcggtgatc aggccgacgg cgatggtcgg caggccgacc
      241 tcggccttga ttcgtgccgc cagcggcacc tggtatcccg gcccgaccgg aatcttctgc
      301 gtcgaagcga cgccgccgct cgagacatgg atcgcggcgc aacctcgcgc ggcgagcgcc
      361 ttgccgaacg cgaccgtgct ttcgacgtcc cagccaccgt cgacccagtc ggtcgccgag
      421 acacggaccc agaccgggct ttgcgccgga aagacggcgc gcaccgcgtc gaagacctcg
      481 agcggaaaac gcatccggtt ctcgaggctg ccgccgtact cgtcctcgcg gcgattggcg
      541 acgggcgaca ggaactgatg cagcagatag ccgtgcgccg catgcaactc gacgccgtcg
      601 aatcccaggc gggcggcgcg gcgggcagcg gcaacgaaat catcgcgcac cttgcgcatg
      661 ccggcatcgc ccagcgcctg cggcgcgtct tcgcccggcg agtgcggcac ggccgaaggc
      721 gcgaccgcgc gccaggcgcc cggctcgccc agcctgatct gcgcgccgcc gtcccacggc
      781 gcacggctcg acgccttgcg gccggcatgt gccagctgca gcgcgacgcg gatcggcgaa
      841 tgggcgcgaa tcgcggcgac gacgcggccg agcgcctgct cgttggcgtc gctgtagagg
      901 ccgaggtcgc cagccgtgat ccgcccctcg gccgacacgg cggtcgcctc gacgacaagc
      961 aggcccgcac ccgagagcgc gagatggccg agatggatga gatgccagtc gcccggcgtc
     1021 ccgtcgctcg cggagtactg gcacatcggt gcgacgacga tgcggttggc gagcggcagg
     1081 gcaccgatcc gccacggttc gaagagtcgg ctcatggcga cgattctggt cggatcggcg
     1141 gcgccccgcc ggcgaccacc ggatgcgggc gggctcgagt cgctcaggat cgagcgggtg
     1201 acgggccggc cgacagccgg cgcgccgcgt cgtcgacgat ctcgcccacc gggcggtcga
     1261 tggccaggcg cagcacgtcg gtttcgtcgt caccgggcgg ctcgagcgtt cgcagctggc
     1321 tggccagcag cgcggccggc atgtagtggg catggcggtg cgcgagtcga ccggcgatca
     1381 acgtctcgct gccgtcgagg aagacgaagc gaagccccgg caccgcggcg cgaatccggt
     1441 cgcggtaggt tcgcttcagc gccgagcagg cgacgacgac gccctggggc cagcgctgcg
     1501 cgtcgcccag gcaggcgccg atccggtcga gccacggcca tcggtcatcg tcctgcagcg
     1561 gtatcgcggc gcgcatcttg gccacgcttt cgggcgaatg caggctatcg ccgtcgatga
     1621 agtgcagccc gagcgccgaa gccagcgcct cggcgatcgt tgtcttgccg gatcccgaaa
     1681 cgcccatgac gacgacggcg atcctttcgg cgtcgcccga aatcggcggc cgggacttcg
     1741 gctctcgtgg cggcgtcagc ctggctggca tcggagttcc ggcgcgcccc gtgactgagg
     1801 ccgctcggtg aatttcacga tagcggagtc ggcgcggcgc cgcaacgctg cgcagcagcg
     1861 agaacggccc ctgtcccgac cggcggtctc gaccttctcc tacgcacgac atcgagacgc
     1921 caggggatca tgcagtggca gaaaaaggag ctctccatgc agacatcaag attcgccgcc
     1981 accgtgctgg ccgcgtcgct tgcggcggcc aacgtggcct gggcgcaagg tgatacgcag
     2041 aacgtcgagc gcgccgatcc gcacattcag cagccgcgcg agccctcccg gacgaattcc
     2101 cgggacaggg gtcagtcgca gatggcgcag cggcacgaca accggggcaa ctcgaacggc
     2161 tggaaccgcg gcaacgatcg ccagcaaggt cgctcgtacg accgtgacgg cccccatgac
     2221 ggctacgttc gcaacgatcg atccgatcat cgcaactggg gcgccgggcc cgatcacaac
     2281 ttctatcggg gcggctacct gccacgtgaa tacaggagcc gccagtacgt ggtcgacgat
     2341 tggcgcggcc accacctgcg gccgccgccg cggggctatc actgggtcca gaccggcggc
     2401 gactacgtcc tcgccgcgat cgccaccggg gtgatcacct cgatcatcct caacgccgcc
     2461 gcgaacaact gacccctggc ccttcgcgac gccgcgccgg agccgcattt tgttcgtgcg
     2521 acgatcgcat ctcgtacgac gggagatgat gtggtgaatc gaaggaccgc gggtcgcgct
     2581 tccctgccgg cgtcctgggc ggccgtggcc ctcgccacgg cgatgctcgc cggctgcgga
     2641 gcgccgcagg ccaggcccga catccagacc gtcgtcaccg cgcgcgtcgc cggcatgagc
     2701 gtcggcgact tcttcgatac cttcggcagg cctcgcagcc ggtcggagcg gggcgacggg
     2761 tcgacggact acctgtggat ctccgagacg aaagcgctcg gcagcggcgc cgccaacgtg
     2821 gacttgagcc gctgcacctt gcgcctcgtt gccgactcgc gcaatcggat cgccagcgtc
     2881 gaagtcgtcc tggacagccc cggctacacc agcacgtcgc gctgcaatga aatcttcgca
     2941 aagaagtgac gcggacgccg gcgcgactgc aagatttcac cagggcgaac cggtccgcag
     3001 cgccgccgaa ttgaccctct cgccttgggc atggccggcg gcacggtcgc aggatcggcc
     3061 gaaaacccgc agaatctggc gtttcgcgcg tccaccctcg cctctgccat gaatgacgat
     3121 ccccaggaac gcgcccgcca gcaggccgtg gacgcgctcg ggctcgattc gttgaccgac
     3181 acggcgtacg acgacatcac ccgcgcggcc gccgagttgt tcggcacgcc gatcgccctg
     3241 atcaccatcc tcgacggcga ccggcagtgg ttcaagtcgc gcg
//
LOCUS       DASRWS010000558         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1683098, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000558 DASRWS010000000
VERSION     DASRWS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683098"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="sucB"
                     /locus_tag="VFF72_10600"
     CDS             <1..795
                     /gene="sucB"
                     /locus_tag="VFF72_10600"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HZV93656.1"
                     /translation="PTAVRGGAAVAALVKPKALPAVASRPAASLGDRPEQRVPMSRLR
                     ARIAERLLQSQSTSAILTTFNEVNMAPVIEMRKRFQEKFEKEHGVRIGFMSFFVKAAV
                     AALKKYPIINASVDGTDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAE
                     FGKMAAEGKLSLDDLTGGTFTISNGGVFGSLLSTPIINPPQSAILGVHATKERAVVEN
                     GQVVARPMNYLAMSYDHRIVDGREAVLGLVAMKDALEDPARLLFDI"
     gene            797..>2134
                     /gene="lpdA"
                     /locus_tag="VFF72_10605"
     CDS             797..>2134
                     /gene="lpdA"
                     /locus_tag="VFF72_10605"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZV93657.1"
                     /translation="MASTPSTTADAGSGAFDVVVIGAGPGGYVAAIRAAQLGFSTACV
                     DEWVNEKGGPAPGGTCTNVGCIPSKALLQSSENFALAGHHFADHGIGLSNLSIDVAKM
                     MARKKAIVKQNNDGIVYLFKKNKVHFFHGRGSLSKKTGAGWEIAVTGAEATTLQARHV
                     IVATGSSARPLPGVPFDEAKILSNDGALCIDAVPKRLGIVGSGVIGLEMGSVWRRLGA
                     EVTILEALPTFLGAVDAQVAAEAQKSFKRQGLEIVLGAKIDAVDTRADDVSVRFVDAK
                     GAAQTLSCDKLIVSIGRIPHTIGLGAEAVGLKLDERGFVSVDADCRTNLDNVWAVGDV
                     VRGPMLAHKAEEEGVAVAERIAGQKPHVDFNTVPWVIYTSPEIAWVGQTEQQLKAAGR
                     PCRTGVFPFMANGRARALGDTTGFVKMIADAKTDEILGVHMIGPMVSELIAEAA"
BASE COUNT          377 a          692 c          715 g          350 t
ORIGIN      
        1 ccgacggcgg ttcgcggcgg cgcggccgtc gctgcgctcg tcaagccgaa ggcccttccg
       61 gcagtggcgt cgcggccggc ggcgagcctc ggcgaccggc ccgagcagcg ggtgccgatg
      121 agccgccttc gcgcacgcat cgccgagcga ctgcttcagt cgcaatcgac gagtgcgatc
      181 ctcaccacgt tcaacgaagt gaacatggcg ccggtgatcg agatgaggaa gcgattccag
      241 gagaagttcg aaaaggagca cggcgtcagg atcggcttca tgagcttctt cgtgaaggcg
      301 gccgtcgcgg cgctcaagaa gtacccgatc atcaacgcgt cggtcgacgg caccgacatc
      361 gtctaccacg gctacttcga tatcggcatc gccgtcggct cgccgcgtgg actggtcgtg
      421 ccgatcctgc gaaatgccga tcagatgagc ttcgccgaca tcgagaagaa gatcgccgag
      481 ttcggcaaga tggccgccga aggcaagctc tcgctcgacg acctgaccgg cggcacgttc
      541 acgatctcga acggcggcgt tttcggctcg ctgctctcga cgcccatcat caatccgccg
      601 cagtcggcaa tcctcggcgt tcacgccacc aaggagcgcg ccgtcgtcga gaacgggcag
      661 gtggtggcgc ggccgatgaa ctacctggcg atgtcgtacg accaccgcat cgtcgatggt
      721 cgcgaagccg tccttggcct ggtggcgatg aaggatgcgc tggaagaccc ggcgcgactg
      781 ctcttcgaca tctgacatgg ccagcacgcc ttcgaccact gccgatgccg gttccggcgc
      841 cttcgatgtc gtcgtcatcg gcgccgggcc gggcggctac gtcgccgcca tccgcgccgc
      901 gcagctcggc ttctcgacgg cctgcgtcga cgaatgggtc aacgaaaaag gcggcccggc
      961 tccgggcggt acgtgcacca acgtcggctg cattccgtcc aaggccttgc tccagtcctc
     1021 ggaaaacttc gccctggcgg gccaccattt tgccgaccac ggcatcggcc tgtcgaacct
     1081 gagcatcgac gtggcgaaga tgatggcgcg caagaaggcc atcgtgaagc agaacaacga
     1141 tggcatcgtc tacctcttca agaagaacaa ggttcacttc ttccatggcc gaggctcgtt
     1201 gtcgaagaag acaggggccg gctgggagat cgccgtaacc ggcgccgagg cgacgacgct
     1261 gcaggcgcgt cacgtcatcg tcgctaccgg atcgagcgcc aggccgttgc cgggcgtgcc
     1321 gttcgacgag gcgaagatcc tctcgaacga cggtgcgctc tgcatcgacg cggtgcccaa
     1381 gaggctgggc atcgtcggct cgggagtgat cggcctggaa atgggttcgg tctggcgccg
     1441 gctcggcgcc gaggtgacga tcctcgaggc attgccgacc ttcctcggcg ccgtcgatgc
     1501 gcaggtcgcc gcggaggcgc agaagtcgtt caagcggcag ggcctcgaga tcgtcctcgg
     1561 cgcgaagatc gacgcggtcg acacccgggc cgacgacgtt tcggtgcgct tcgtcgacgc
     1621 caagggcgct gcgcagacgc tgtcgtgcga caagctcatc gtttcgatcg gacgcatccc
     1681 ccacaccatc ggcctcggcg ccgaggctgt cggattgaag ctcgacgagc gcggcttcgt
     1741 gagcgtcgat gccgattgcc gcaccaacct cgataacgtc tgggcggtcg gtgacgtcgt
     1801 ccgcggcccg atgctcgcgc acaaggccga ggaggagggc gtcgccgtcg ccgagcgaat
     1861 cgccgggcag aaaccccacg tcgacttcaa caccgtaccc tgggtcatct ataccagccc
     1921 cgagatcgcc tgggtcggcc agaccgagca gcagttgaag gcggcggggc gaccttgtcg
     1981 cacgggcgtc tttccgttca tggcgaacgg ccgggcgcgg gcattgggtg acacgacggg
     2041 cttcgtcaag atgatcgcgg atgcgaagac cgacgagatt ctcggcgtgc acatgatcgg
     2101 accgatggtc tcggagttga tcgccgaagc cgcg
//
LOCUS       DASRWS010000559         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3413502, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000559 DASRWS010000000
VERSION     DASRWS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3413502"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="radA"
                     /locus_tag="VFF72_10610"
     CDS             complement(<1..1259)
                     /gene="radA"
                     /locus_tag="VFF72_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZV93658.1"
                     /translation="MAKEKSNFACTECGGTSPRWQGKCPHCGAWNTLIEAVVESAAKN
                     RFAASSRGLTPAESVATLSEIEATDTDRQPTGIDELDRALGGGIVAGGVILIGGDPGI
                     GKSTLLLQALDVLSRVCRVLYVTGEESGAQVALRSRRLGLGRSAVRVMAEIQLEKIMA
                     TIEHERPAVCVVDSIQTVYSDALTSAPGSVAQVRECAAQLTRLAKASGTTVILVGHVT
                     KDGALAGPRVMEHIVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGV
                     ANPSAIFISTHGEPVAGSCVLVTLEGTRPLLVEVQALVDAGGPSPRRLSVGLDRDRLA
                     MMLAVLHRHAGIAALDQDVFVNAVGGVRISEPAADLAVMLAIQSSLRGKALPRGFIAF
                     GEVGLAGEVRPAPRGQERLREAAKLGFS"
     gene            complement(1303..2877)
                     /locus_tag="VFF72_10615"
     CDS             complement(1303..2877)
                     /locus_tag="VFF72_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HZV93659.1"
                     /translation="MKTRIVHAPEAFAMHRRDAVRALGALGAALALPTFAAAPAAASD
                     GAAFDAAVADRPWLAPFKGIDDSDPSRSDLRCGELAVSGRWPAALRGRFYRNGPAIFE
                     RDGQRYRHWFDGDGMVQQFTFSGRGVSHLGRLVRTPKLAAERQAGRFLYSAFGTGIQS
                     EAPIQGPDSMNVANTNAIEHAGRILAMWEGGSAFALHPDGLATQGPVTWKEGFEQVPF
                     SAHPKLDPASGALWNIGTFGEKIVVWHIDAGGRLAGVQVGESPYPNGMAHDVAVTARY
                     IVLPLPPVKMNYAAIARGATPEQAFDFQRSEPLRILVMRKDDIRERRVFELPARMVFH
                     VGNAFERPDGRIALTYVGAKDDEFLIHGAVAMVAGHVAGPTGSQLQSALLDMTSGRAT
                     VEEMPGSIEFPRIDPRRIGAPARHLISAASWKDFGERRGALFHGVQVRDLQTGRVDRF
                     DYGERMVVEEHIVVPKPGATHELDSWLLGTTYDVRRKVTTVNVLEMKRVADGPIAQAT
                     LPYWLPYGFHGNFTPA"
     gene            complement(2895..3332)
                     /locus_tag="VFF72_10620"
     CDS             complement(2895..3332)
                     /locus_tag="VFF72_10620"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HZV93660.1"
                     /translation="MNQRCLSSFAAVSVGAVLSALAVVASPARAATCVDLEVQNLRAG
                     GTLMLAAYADASTFDKSPVVALQMKVDGESMRFPLCGLQGDHVALTLYQDLNGNGKLD
                     KNVLGIPSEPWGASGRPAAMSAPTWATTAVPLDASTVVVRLSK"
     gene            complement(3606..4010)
                     /gene="arfB"
                     /locus_tag="VFF72_10625"
     CDS             complement(3606..4010)
                     /gene="arfB"
                     /locus_tag="VFF72_10625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZV93661.1"
                     /translation="MSTVPVVAESDVEWTAVRAQGAGGQNVNKVSSAVHLRFDVAASS
                     LPDAVKARLLGGTDRRINDEGVVVIKAQRHRSQDLNRRDALDRLNALVAQAAEVPLVR
                     RPTRPTRASQRRRLEGKSQRAEVKAGRSKISG"
BASE COUNT          581 a         1408 c         1423 g          610 t
ORIGIN      
        1 gaaaagccga gcttggccgc ttctcgcaac cgttcctggc cgcgcggtgc cggtcgcact
       61 tcgccggcca gaccgacctc gccgaaagcg atgaagccgc gcggcagcgc cttgccgcgc
      121 aggctcgact ggatcgccag catcaccgcg aggtcggcgg ccggctcgct gatgcgcacg
      181 ccgccgacgg cattgacgaa gacgtcctgg tcgagcgcgg cgatgccggc gtggcgatgc
      241 agcaccgcga gcatcatcgc cagccgatcg cgatcgagcc cgaccgagag ccggcgcggg
      301 ctcgggccac ccgcatcgac gagcgcctgc acctcgacga gcaagggcct ggtgccttcg
      361 agcgtgacca gcacgcacga tccggcgacc ggctcgccgt gcgtcgagat aaagatcgcg
      421 ctcgggttgg cgacgccctt caggcccttc tccgtcatcg cgaagacgcc gatctcgttg
      481 accgcgccga agcggttctt gatcgcccgc accaggcgaa agctcgaatg cgtatcgccc
      541 tcgaaataca gcaccgtgtc gacgatgtgc tccatgacgc gcgggccggc caaggcgccg
      601 tccttggtga cgtggccgac gaggatcact gtcgtgccgc tcgccttggc cagccgcgtc
      661 agctgcgcgg cgcattcgcg cacctgcgcc accgagcccg gtgccgacgt cagcgcatcg
      721 ctgtagacgg tctggatcga atcgacgacg cagaccgccg ggcgctcgtg ctcgatcgtc
      781 gccatgatct tctcgagctg gatctcggcc atcacgcgga ccgcgctcct gccgaggccg
      841 agccggcgcg agcgcagcgc cacctgcgcg ccgctctctt cgccggtgac gtagagcacc
      901 cggcagacgc gcgacaggac atcgagcgcc tgcagcaaca gcgtcgactt gccgatgccc
      961 gggtcgccgc cgatgaggat gacgccgccg gcgacgatgc cgccgccgag cgcccggtcg
     1021 agctcgtcga tgccggtcgg ctggcggtcg gtgtcggtcg cctcgatttc cgagagcgtc
     1081 gccaccgact cggcgggcgt gaggccgcgc gaagaggcgg cgaaccggtt cttcgccgcg
     1141 ctttccacga ccgcctcgat cagcgtgttc caggcgccgc agtgcgggca cttgccctgc
     1201 cagcgcgggc tcgtgccgcc gcactcggtg cacgcgaagt tcgacttttc cttggccatc
     1261 ggcgtcgatt gtcgacgctc gcctcgctgg tgtcggagag gctcaggccg gcgtgaagtt
     1321 tccgtggaag ccgtacggca gccagtacgg cagcgtcgcc tgggcgatcg gcccgtcggc
     1381 gacgcgtttc atctcgagca cgttgacggt cgtcaccttg cgcctgacgt cgtaggtcgt
     1441 gccgagcagc cacgaatcga gctcgtgcgt tgcaccgggt ttcggaacga cgatgtgctc
     1501 ctcgacgacc atgcgctcgc cgtagtcgaa ccgatcgacc cggccggttt gcagatcgcg
     1561 gacctgcacg ccgtgaaaga gcgcgccgcg ccgctcgccg aagtccttcc agctcgccgc
     1621 gctgatgagg tggcgtgccg gtgcgccgat gcgacgcgga tcgatccgcg ggaactcgat
     1681 gctgcccggc atctcctcga ccgtggcgcg gccgctcgtc atgtcgagca acgccgattg
     1741 caattgcgat ccggtcggcc cggcaacatg gccggcgacc atcgccaccg cgccgtggat
     1801 caggaactcg tcgtccttgg cgccgacata cgtgagggcg atccggccat cggggcgttc
     1861 gaaggcgttg ccgacgtgaa agaccatcct ggccggcagc tcgaagacgc ggcgctcgcg
     1921 aatgtcgtcc tttctcatca cgaggatgcg caggggctcg ctgcgctgga aatcgaacgc
     1981 ctgctcgggc gtcgcgccgc gggcgatcgc cgcgtagttc atcttcaccg gcggcagcgg
     2041 caagacgatg tagcgcgcgg tcacggcgac gtcgtgagcc atgccgttcg gatagggaga
     2101 ttcgccgacc tgcacgccgg ccagccggcc gccggcgtcg atgtgccaga cgacgatctt
     2161 ctcgccgaag gtgccgatgt tccagagcgc gccgctcgcc gggtcgagct tggggtgcgc
     2221 cgagaacggc acctgctcga agccctcctt ccaggtaacg ggcccctggg tcgcgagccc
     2281 gtccgggtgg agggcgaacg ccgatccgcc ttcccacatc gccaggatcc gccccgcgtg
     2341 ctcgatggcg ttggtgttgg cgacgttcat cgagtcgggc ccctggatcg gcgcctcgct
     2401 ctggatgccg gtgccgaagg cgctgtagag gaaacgtccg gcctgtcgtt cggcggcgag
     2461 cttgggcgtg cgcaccagcc ggccgaggtg cgacacgccg cgacccgaga aggtgaactg
     2521 ctggaccatg ccgtcgccgt cgaaccagtg ccggtagcgc tggccgtcgc gctcgaagat
     2581 tgccggcccg ttgcgataga agcggccgcg cagcgccgcc ggccagcggc cgctgacggc
     2641 gagttcgccg caacgcaggt cgctgcgcga cgggtcgctg tcgtcgatgc ccttgaaggg
     2701 cgccagccac ggccggtcgg ctacggcagc gtcgaatgcg gcgccgtcgc tcgcggccgc
     2761 cggcgcggca gcgaaggtcg gcagcgccag ggcggcgccg agggcgccga gggcccggac
     2821 ggcgtcgcga cgatgcatgg cgaaggcttc gggagcgtgg acgatgcggg tcttcatgac
     2881 ggtctccttg cgaatcactt ggaaagcctg acgacgaccg tcgaggcgtc gagcggcacg
     2941 gccgtggtcg cccaggtcgg cgccgacatg gccgccggtc gacccgaggc gccccatggc
     3001 tcgctcggga tgccgaggac gttcttgtcc agcttgccgt tgccgttcaa gtcctggtag
     3061 agcgtcagcg cgacgtggtc gccctgcagg ccgcacaagg gaaagcgcat cgactcgccg
     3121 tcgaccttca tctgcagggc gacgaccggc gacttgtcga aggtcgaggc gtcggcgtag
     3181 gcggcaagca tcaacgtgcc gccggcacgc aggttctgca cctcgaggtc gacgcaggtc
     3241 gcggcgcggg ccggactggc gacgaccgcg agcgcggaca gcacggcgcc gacgctgacg
     3301 gcggcgaagg aggagaggca gcgctggttc atggcgatct cgtgagtggc gatgcggtca
     3361 ctctaggcag cgcctcgccg cgtcgcgacg ccgaggcgac gaaacgcagg gcatcggcgc
     3421 gaagcgtcgg cggtgcggtg ccagtcgcgg cgcccatcgc agcatccgtc gcgatggccg
     3481 ccgcggccgc aggcgcgccg acttccgcgg tgcagtcctt cagcggccgg tgctttcccc
     3541 ggggaggcgg tccggcaatc cggcaggcgg cgatgccgaa cccgtcggca tcatcgtcac
     3601 gaacctcagc cgctgatctt gctgcggccg gccttcacct cggcgcgctg gctcttgccc
     3661 tcgagacgcc gccgctgcga cgcccgcgtc ggccgggtcg gccgacgcac gagcggcact
     3721 tctgcggctt gcgcgaccag cgcgttcagg cgatcgagcg catcgcgccg gttcaggtcc
     3781 tggctgcggt gacgctgcgc cttgatgacg acgacgcctt cgtcgttgat gcgccgatcg
     3841 gtcccgccga gcaggcgcgc cttcacggca tcgggcagcg acgacgcggc gacgtcgaag
     3901 cgcaggtgga ccgcgctcga gaccttgttg acgttctgcc cgccggcgcc ctgcgcacgc
     3961 accgcggtcc actcgacgtc gctttcggcg acgaccggca cggtgctcat gcgctcctgc
     4021 cg
//
LOCUS       DASRWS010000560         6568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3962932, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000560 DASRWS010000000
VERSION     DASRWS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3962932"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFF72_10630"
     CDS             complement(<1..346)
                     /locus_tag="VFF72_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HZV93662.1"
                     /translation="MAAVAPFSFDNSFARELEGFHTPWRPETVPAPKTLFFNEPLAQE
                     LGLDVAALSGAPGAALLSGNALPEGAEPIAQAYAGHQFGGFSPQLGDGRAVLLGEVID
                     RAGQRRDIALKGS"
     gene            489..1091
                     /locus_tag="VFF72_10635"
     CDS             489..1091
                     /locus_tag="VFF72_10635"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HZV93663.1"
                     /translation="MSTSRHLRSQPDTSAKAAEGESDSARADSGDWAHRGVVPREGQG
                     TGWVVVADEAIARFLTTTDVPGELDAVEALTDPAAHAREGELHVNDAGRRGGRVSSDS
                     GRGAGNGSGVASVTASAGDDNRHLEAQAFARRVAQHLSMALQHKRYASLRIVAAPRFL
                     GALRGELDAAVKAIVVEEIDKDLIHMSDADIAGRLSKRAA"
     gene            1105..1458
                     /locus_tag="VFF72_10640"
     CDS             1105..1458
                     /locus_tag="VFF72_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HZV93664.1"
                     /translation="MPLREPSLIASWHAHVYFDASSREAARALRQTIETTLSGQVVVG
                     RFHERPVGPHPMWSYQLAVAAAEFSVVVGWLALNHGGLDVFIHPNTGDALRDHRDSAI
                     WIGRSHILNLAALGG"
     gene            complement(1502..2566)
                     /locus_tag="VFF72_10645"
     CDS             complement(1502..2566)
                     /locus_tag="VFF72_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV93665.1"
                     /translation="MTGSKQAPKQLQLQPQLQPQPQPQPQMQSVLDRLVRARSGLPSR
                     YELPFEQAREHLLLEREPWLVDGPECAGRDRKVECEGRAVSMRIVMPPRMSPRRILVY
                     LHGGGWCVGSSRTHDAIVRRLAGALGCEAWSIDYALAPESPYPQGLRDVVAAIRLAAA
                     EHPQADLIVAGDSAGANLALDAALQMRDAGDDSIAALLLFYGVYTDDCSGPSMEAYGD
                     GRFGLSIRAHRRYLDAYRGAATAQPGEEGAQGADAAASEPGRRGTDVPVFALSPLASL
                     AGLPPAWLTVAELDILRDQSHALATRLRLAHVPVEVDEVPGVIHGFLAYGQQLDAVGE
                     ALGRAAVWARVIADGPAPPS"
     gene            complement(2599..2982)
                     /locus_tag="VFF72_10650"
     CDS             complement(2599..2982)
                     /locus_tag="VFF72_10650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV93666.1"
                     /translation="MNQHNPRSIAGPLGAYSHAVSAPGAGRWLHIAGQIGLRADGTLA
                     PDFVAQAEAAWSNLVAILADAGMGVEDLVKVTHYLVRGTDLVEYNPVRTRHLGEARPA
                     STLIVVHALARPEWMIEIDAVAWKA"
     gene            complement(2993..3889)
                     /locus_tag="VFF72_10655"
     CDS             complement(2993..3889)
                     /locus_tag="VFF72_10655"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV93667.1"
                     /translation="MESDGESAARPASVDDGTQASPARAVDEALEREYNLRIRHPERG
                     AAYDDYRRRSVALRERETEHRARRYGEAANCVIDFFPARVVAPAPLFVFIHGGYWRAL
                     DRGIFSFLARPWLDRGVHVAMPGYDLAPAVSVRAIADQIHAALDGLLGDAAALQIDAT
                     RCIVGGHSAGAHLGALVLSEKRKGSAIGFVGVSGIYALAPLLSTSINFDVRLDADEAR
                     ALSPSPARADRATRYLCAVGGAETDGFRRQTHDYVAALRERGCHARSLEVPGRTHFDV
                     LDDLADADAGLFRSAFALLSPA"
     gene            complement(3879..4592)
                     /locus_tag="VFF72_10660"
     CDS             complement(3879..4592)
                     /locus_tag="VFF72_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93668.1"
                     /translation="MLEVDSLGAGYGAADVLHGVSITVPGGSFAALIGANGAGKSTTM
                     KAIAGLLRPVRGEIRFAGERIERAAAARIVRRGLALVPEGRRVFGPMSVRENLQMGAF
                     TRLFPRRAADAGQRLDLVLETFPRLQERLEQAAGTLSGGEQQMLAIGRALMSEPRLLL
                     LDEPSMGLAPVIVEQVFEALKQLQRGGLTLLVSEQNAAAALAHADQGYVIENGRIVIA
                     ASAADLRGNDRVREAYLGI"
     gene            complement(4639..5403)
                     /locus_tag="VFF72_10665"
     CDS             complement(4639..5403)
                     /locus_tag="VFF72_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93669.1"
                     /translation="MAALELGAVSVAFGGLRAVDDLTCSVAKGAIKGIIGPNGAGKTT
                     LFNAISGITAPDRGRIILDGVDITTMRPHRRAGLGLARTFQNLQLFAGMSLLENVMIG
                     AHTRLHAGWAAGLFGLSRAEEQRAEALARDKLGLLGLEARAAELAGNLGFADAKLLEI
                     ARALASEPRLLLLDEPIAGVPPVDQNRIADVIRTVNASGVTIVLVEHNMRVVMNVCHD
                     ILVMNHGARLAEGEPASVARNPEVIAAYLGAGATHA"
     gene            complement(5405..6322)
                     /locus_tag="VFF72_10670"
     CDS             complement(5405..6322)
                     /locus_tag="VFF72_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93670.1"
                     /translation="MAGNDYVLSLGVFFFINLLLIGGLNLVMGYGGQISLCQGGFFGV
                     GAYVSGVLALKYGVPSALGVCAAIAGAALSAFVIGLPALRLRGNYLAMATLGFNAILS
                     VLFNELVSLTGGPNGLSGIPPIGVAGFAFDSPGRFFWLAWAAGALLMALLAMLLASRA
                     GRALRAVAGSEVAAASMGVDPFRSKLAAFMFSAACAGLAGALYAHFNQYASPETFNFS
                     TSVLLVVMVALGGWGHYWGPVFGAALFTAVPELLRRFQDAELLVFGVGMIVVLLFLPS
                     GVAGVSERIRARPAGRRAASLRASGRIGG"
BASE COUNT         1097 a         2250 c         2368 g          853 t
ORIGIN      
        1 cggagccctt cagggcgatg tcgcgacgct ggccggcacg atcgatcacc tcgccgagga
       61 gcacggcgcg gccatcgccc agctgcggcg aaaagccgcc gaattgatgc ccggcgtagg
      121 cctgggcgat cggctcggct ccctcgggca gggcattgcc cgacaacagc gcggcgccgg
      181 gtgcgccgga gagggccgcg acatcgaggc cgagctcctg cgccagcggc tcgttgaaga
      241 acagcgtctt cggcgccggc accgtctccg gccgccacgg cgtgtgaaag ccctcgagct
      301 cgcgggcgaa ggagttgtcg aacgaaaaag gtgccacagc ggccatgggc gattcaagaa
      361 gaccccaggc gcggagtcgc aggatggagt ttgaacgggt tcgaggaatc ggacccggac
      421 agggtcgaag tggcggcagg aacgtcgatt gcaatgcaca cggtacgtcc aacatcacga
      481 gaaccctcat gtcgacttcc aggcaccttc gatcacagcc cgacacttcc gccaaggcag
      541 ccgagggcga gtcggacagc gcccgcgccg attccggcga ctgggcgcac cggggcgtcg
      601 ttccccgcga aggtcagggc accggctggg tcgtggttgc cgacgaggcc atcgcacgat
      661 tcctgacgac caccgacgtg cccggcgagc tcgacgcggt cgaggccttg accgacccgg
      721 cggcgcacgc cagggaaggc gagctccatg tcaacgatgc ggggcggcgc ggcgggcgcg
      781 tctcgagcga ctcgggccgc ggcgcgggaa acggctcggg cgtcgccagc gtcacggcct
      841 cggccggcga cgacaaccgg catctcgaag cccaggcctt cgccaggcgc gtggcccagc
      901 atctttcgat ggcgttgcag cacaagcgct acgccagctt gcgcatcgtc gcggcgccga
      961 gattcctggg cgcgctgcgc ggcgagctcg acgccgccgt caaggcgatc gtggtcgagg
     1021 agatcgacaa ggacctcatc cacatgagcg atgccgacat cgccggccgg ctctcgaagc
     1081 gcgccgcctg acaatcgccg cgtgatcccg cttcgcgagc cgtccctcat cgccagctgg
     1141 catgcgcacg tctacttcga cgcgtcgagc cgcgaggccg cccgggccct gcggcagacg
     1201 atcgaaacaa cgttgtcggg gcaggtcgtg gtgggacgat tccacgaaag gccggtcggc
     1261 ccccatccga tgtggagtta tcagctcgcc gtcgccgccg cggaattctc cgtggtcgtg
     1321 ggctggctgg cgctgaatca cggcggactc gacgtcttca ttcatccgaa caccggcgac
     1381 gccttgcgcg accatcgcga cagcgcgatc tggatcggcc gctcgcacat cctgaacctc
     1441 gcggcgctgg gcggttgatc ctgtttcctt cggtgcaacg cgccggctgc catgcaggcc
     1501 gtcaggaggg tggggccggg ccgtcggcga tcacgcgcgc ccataccgca gcgcggccga
     1561 gggcctcgcc gacggcgtcg agctgctggc cataggcaag gaatccgtgg atgacgccgg
     1621 gcacctcgtc gacctcgacg gggacgtgcg cgagccgaag acgtgttgcc agcgcgtgcg
     1681 actggtcgcg caggatgtcg agctccgcga ccgtgagcca tgccggcggc aaacctgcca
     1741 gcgaggccag gggcgacaac gcgaagaccg gcacatcggt gccgcgccga ccgggctcgc
     1801 ttgccgcggc atctgcgccc tgtgccccct cctcgccagg ttgcgccgtc gctgcgccgc
     1861 ggtacgcgtc gagatagcgc cgatgcgcgc gaatcgacag gccgaagcgg ccgtcgccgt
     1921 aggcctccat gctggggccg ctgcagtcat cggtgtagac gccgtagaag agcagcagcg
     1981 cggcgatcga atcatcgccg gcgtcgcgca tctgcaaggc ggcatcgagg gccaggttgg
     2041 cgcccgccga atcgccggcg acgatcaggt cggcctgcgg atgctcggcg gcagcgagcc
     2101 tgatcgcggc gacgacgtcg cgaagcccct gcggataggg cgattcgggt gccagagcgt
     2161 agtcgatcga ccaggcctcg cagccgagcg cacccgcgag gcggcgcacg atcgcgtcgt
     2221 gggtgcgcga cgagccgaca caccagccgc cgccgtgcaa atagaccagg atgcgccgcg
     2281 gcgacatccg cggcggcatg acgatgcgca tcgacacggc gcgaccttcg cactcgacct
     2341 tgcggtcgcg accggcacac tcgggaccgt cgacaagcca gggctcgcgc tcgagcagca
     2401 aatgctcgcg agcctgctcg aacggcagct cgtagcgact cggcaggcca gaccgtgcgc
     2461 ggacaaggcg gtccaggacg gactgcatct gcggctgcgg ctgcggctgc ggctgcagct
     2521 gcggctgcag ctgcagctgc ttgggcgctt gcttcgagcc cgtcacttcg ccttctgccc
     2581 gactgatccg aacgggcgct aagccttcca ggccacggcg tcgatctcga tcatccactc
     2641 cggtcgcgcc aaggcatgga cgacgatcag cgtcgatgcc ggtctcgcct cgccgagatg
     2701 cctggtgcgg accgggttgt actcgaccag gtcggtgccg cgaacgaggt agtgcgtgac
     2761 cttgacgagg tcctcgacgc ccatgccggc atcggcgagg atcgcgacca ggttgctcca
     2821 tgcggcctcg gcctgcgcga cgaagtcggg agcgagcgtg ccgtcggcgc gaagcccgat
     2881 ctgcccggcg atgtgcagcc agcggccggc gccgggcgcc gaaacggcat gcgagtaggc
     2941 gccgagcggg ccggcgatgg agcgagggtt gtgctggttc atgacgatct tctcaggcag
     3001 gggaaagcag agcaaaggca ctgcgaaaca gcccggcgtc ggcatcggcc aggtcgtcga
     3061 gcacgtcgaa atgggtgcgg ccgggtactt cgagcgaacg cgcgtggcaa cctcgctcgc
     3121 gcaaggcagc gacgtagtcg tgcgtctggc ggcgaaagcc gtcggtttcg gcccctccga
     3181 cggcgcagag atagcgggtc gcgcgatcgg cgcgagccgg cgaggggctc agggcgcggg
     3241 cctcgtcggc gtcgaggcgg acatcgaagt tgatgctcgt cgaaagaagc ggcgccagcg
     3301 catagatgcc gctgacaccg acgaagccga tggccgaccc cttgcgcttc tcgctcagca
     3361 ccagggcgcc gaggtgggcc ccggccgaat ggccgccgac gatgcagcgg gtggcatcga
     3421 tctgcagcgc cgccgcatcg ccgagcagtc cgtcgagcgc ggcgtggatc tggtcggcga
     3481 tcgcgcgcac gctgaccgcc ggcgcgaggt cgtagccggg catcgccacg tggacgccgc
     3541 ggtcgagcca cggtcgggcc aggaacgaga agatgccgcg gtcgagggcg cgccagtagc
     3601 ctccgtggat gaagacgaag agcggcgccg gggcgacgac gcgtgccgga aagaagtcga
     3661 tcacgcagtt cgccgcctcg ccgtaacgac gcgcacggtg ctcggtctcg cgctcgcgca
     3721 gggcgacgct gcggcgtcga tagtcgtcgt acgccgcgcc gcgctcggga tgacggatgc
     3781 gcaggttgta ttcgcgctcc agcgcctcgt cgacggcgcg cgcgggcgat gcctgcgtcc
     3841 cgtcgtcgac cgacgccggg cgcgccgccg actctccgtc agattccaag ataggcctcc
     3901 cggacccggt cgttgccgcg cagatcggcg gcgctcgcgg cgatgacgat gcgcccgttc
     3961 tcgatgacgt agccctgatc ggcgtgggcc agggcggccg cggcgttctg ctccgacacg
     4021 aggagcgtga ggccgccgcg ctgcagttgc ttcagtgcct cgaagacctg ctcgacgatg
     4081 accggcgcca ggcccatcga cggctcgtcg agcagcaaca gccgcggctc gctcatcagc
     4141 gcgcggccga tcgccagcat ctgctgctcg ccccccgaca gcgtgccggc cgcctgctcg
     4201 agccgctcct gaaggcgcgg aaaggtctcg aggacaagat cgagccgctg gccggcgtcc
     4261 gccgcacgcc gcggaaaaag acgcgtgaac gcgcccatct gcaggttctc gcgaacgctc
     4321 atcgggccga acacgcgccg gccttcgggc acgagcgcca gcccgcggcg aacgatgcgc
     4381 gccgccgcgg cgcgctcgat ccgctcgccc gcgaagcgga tctcgccgcg gaccggtcgc
     4441 aggaggccgg cgatcgcttt catcgtcgtc gacttgccgg cgccattggc gcctatcagg
     4501 gccgcgaagg atcctccggg caccgtgatc gacacgccgt gcagcacgtc ggccgcgccg
     4561 tagcccgcgc cgagcgagtc gacctcgagc atcggcgccg ccgcgatcga cggggcgcgt
     4621 tcgcggcgag gcgccgggtc acgcatgggt tgctcccgcg cccaggtagg cggcgatcac
     4681 ttcgggattg cgcgccacgc tcgccggctc gccttcggcg agacgggcgc cgtggttcat
     4741 gaccaggatg tcgtggcaca cgttcatgac gacgcgcatg ttgtgttcga cgaggacgat
     4801 cgtcacgccg ctcgcattga ccgtgcggat gacgtcggcg atgcggttct ggtcgaccgg
     4861 cggcacgccg gcgatcggct cgtcgagcag caacagccgt ggctcggagg cgagggcccg
     4921 ggcgatctcg agcagcttgg cgtcggcgaa gccgaggttg ccggcgagct cggcggcgcg
     4981 tgcctcgagg ccgaggaggc cgagcttgtc gcgggcaagc gcttcggcac gttgttcctc
     5041 ggcgcgcgac agcccgaaga gaccggccgc ccaaccggca tgcagccggg tgtgcgcgcc
     5101 gatcatcacg ttctcgagca ggctcatccc ggcgaagagc tgcaggttct ggaacgtcct
     5161 tgcgaggccg agaccggcgc gccggtgtgg ccgcatcgtg gtgatgtcga cgccgtcgag
     5221 gatgatccgg ccgcggtcgg gcgcggtgat gccggagatc gcgttgaaga gtgtcgtctt
     5281 gccggcaccg ttcgggccga tgattccctt gatggcgccc ttggcgacgc tgcaggtcag
     5341 gtcgtcgacg gcgcgcaggc cgccgaaggc caccgacacc gcgccgagct cgagcgccgc
     5401 cacttcagcc gccgatccgt ccggaggcgc gaagcgaggc cgcgcgccgt cccgcgggcc
     5461 gtgccctgat ccgctccgac acgccggcca cgcccgacgg gagaaagagc aggacgacga
     5521 tcatgccgac gccgaagacg agcagctcgg catcctggaa gcggcgaagc agctcgggca
     5581 ctgcggtgaa aagcgccgcg ccgaagaccg ggccccagta gtgaccccac ccgccgagcg
     5641 ccaccatcac gacgagcagc acggaggtcg agaagttgaa ggtctcgggc gacgcgtact
     5701 ggttgaagtg cgcgtagagc gcgccggcga ggccggcgca ggcggccgag aacatgaacg
     5761 cggcgagctt gctgcgaaac ggatcgacgc ccatgctcgc cgcagcgacc tcgctgccgg
     5821 caaccgcgcg cagcgcgcgg ccggcccggc tggcgagcag catggcgagc agcgccatca
     5881 gcaatgcgcc ggccgcccac gccagccaga agaagcggcc gggggaatcg aaggcgaagc
     5941 cggcgacgcc gatcggcgga atgccggaca ggccgttcgg gccgccggtc agcgagacga
     6001 gctcgttgaa gagcaccgaa aggatcgcat tgaagccgag cgtggccatc gccaggtagt
     6061 tgccgcgcag ccgcagcgcc ggcaggccga tcacgaacgc cgagagcgcg gcgccggcga
     6121 tcgcggcaca gacaccgagc gccgacggca ccccgtattt cagcgcgagc acgcccgaca
     6181 cataggcgcc gaccccgaag aagccgccct ggcaaagcga gatctggccg ccgtatccca
     6241 tgacgagatt caggccgccg atcagcagca ggttgatgaa gaagaagacg cccaggctca
     6301 gcacgtagtc gttgccggcc acgaacggcc atgccgccag gagcagccag acgaggccga
     6361 cgtgacgcag gcgctgccgg cacagcagcg ccggcgaaaa gcgcgacgac gaggacgagg
     6421 gcttcagacg cgctccacct cgccctggcc gagaattccg gcgggccaca ggaaaagcgt
     6481 gagcagcaac gcgacgaaag cgaccgcgtc cttgaagtgc gacgagatca ggccgccgga
     6541 gagcgcttcg aggcaaccga gcagaacg
//
LOCUS       DASRWS010000561         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_511729, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000561 DASRWS010000000
VERSION     DASRWS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511729"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFF72_10675"
     CDS             complement(<1..442)
                     /locus_tag="VFF72_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZV93671.1"
                     /translation="MGHGSWLTSVLIYLGAAVVAVPVARFLGLGSIIGYLGAGILIGP
                     WGLRFVTDPKEMLEFAEFGVVLMLFLVGLELEPQRLWSLRKSIFGWGVFQLAGSALLL
                     AAGAVAFGVDWRLGVVAALGLAMSSTAIGLGVLAERNLLATTSGE"
     gene            complement(442..1134)
                     /locus_tag="VFF72_10680"
     CDS             complement(442..1134)
                     /locus_tag="VFF72_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZV93672.1"
                     /translation="MAESPVDPSAAAAAAAPPAGAEVLVLVAHPELEQSRANRRLLGE
                     ARALQAAGQPGRIEVRDLYALYPDYLVDVAAEQAALAAARLVVWQQPIRWYGMPPLLK
                     LWLDDVLAFGWAYGPGGHALRGKDLWLVASTGGPEDSYRPGSYNRYFFDAFLPPYEQT
                     AALCGMRFMPPLLLHGAHKASEGDLAAHARTYAQRLADYPHWPEIEEIEACPQCVVPL
                     TARPIDNVMEAA"
     gene            1218..1832
                     /locus_tag="VFF72_10685"
     CDS             1218..1832
                     /locus_tag="VFF72_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZV93673.1"
                     /translation="MPVIVVANPKGGVGKTTMSTNLAGYLASRGHAVMLGDVDRQQSS
                     RGWLAHRPAGLPKISAWETAPGDVVRPPKGTTHVVLDTPAGLHGKRLDEVMKLADKVL
                     IPLQPSIFDINATHEFIGQLLAHRRSPQVQLAVVGTRTREGTIATDQLRHFLESMRVP
                     LLAFLRDTQNYVHLAAHGLTLWDVSSVRFERDREQWEPIIVWVN"
     gene            1850..2302
                     /locus_tag="VFF72_10690"
     CDS             1850..2302
                     /locus_tag="VFF72_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZV93674.1"
                     /translation="MRTFATLAELQHLVGQELGVSDWITVTQERIQLFADATGDHQWI
                     HLDRERAKAGPFGTTIAHGFLTLSLLPEMGAKAFAVADTKMGVNYGLNRVRFPAPVPS
                     GSRIRGRFTLSKFEAVDGGAQLVIEVKMEREGSDKPVCVAESVSRRYT"
BASE COUNT          417 a          822 c          875 g          333 t
ORIGIN      
        1 tctcgcccga ggtggtggcc agcaggtttc gctccgcgag cacgcccagg ccgatcgcgg
       61 tcgacgacat cgccaggccg agcgcggcga cgacgccgag ccgccagtcg acgccgaagg
      121 cgacggcgcc ggcggccaga agcagggccg agccggcgag ctggaagacg ccccagccga
      181 agatcgactt gcgcagcgac cacagtcgct gcggctcgag ctcgaggccg accaggaaca
      241 gcatgagcac gacaccgaac tcggcgaact cgagcatctc cttgggatcg gtgacgaagc
      301 gcagtcccca cgggccgatc aggatgccgg cgccgaggta gccgatgatc gagcccagcc
      361 cgaggaagcg ggcgaccggt accgcgacga ccgcggcgcc cagatagatg aggacgctgg
      421 tgagccagga gccgtgtccc atcaggcagc ttccatgacg ttgtcgatcg gccgcgccgt
      481 cagcggcacg acgcattgcg ggcatgcctc gatctcctcg atctcgggcc agtgcggata
      541 gtcggcgagc cgctgcgcgt aggtgcgcgc atgggccgcg agatcgccct cgctcgcctt
      601 gtgcgcgccg tgcagcagca ggggcggcat gaagcgcatt ccgcacagcg ccgcggtctg
      661 ctcgtagggc ggcaggaagg cgtcgaagaa atagcggttg tagctgcccg gccggtacga
      721 gtcctcgggg ccgccggtgg aagcgacgag ccacaggtcc ttgccgcgca gcgcatggcc
      781 gccgggcccg taagcccagc cgaacgcgag cacgtcgtcg agccagagct tgagcagcgg
      841 cggcattccg taccagcgta tcggctgctg ccagacgacc aggcgcgccg cggcgagcgc
      901 cgcctgctcg gcggcgacgt cgacgaggta atcgggatag agcgcgtaga ggtcgcggac
      961 ctcgatccgg ccgggctgcc cggcggcctg cagcgcgcgc gcctcgccga ggagccggcg
     1021 gttggcgcgc gactgctcga gctccgggtg ggcgacaagg acgagaacct cggcgccggc
     1081 aggcggcgcg gcggccgccg cggcggccga aggatcgacg ggggattcgg ccatgcgggc
     1141 cctcgctggt aagtcggtat ggcgcggcag cgccggtcgc cgctaacata accgcaaatc
     1201 cgaaaacgga gagccgcatg ccggtgatcg tggtcgccaa tcccaagggg ggagtcggca
     1261 agacgacgat gtcgacgaat ctcgcgggct acctcgcctc gcgcggccac gccgtcatgc
     1321 tgggcgatgt cgaccgccag cagtcgtcgc gcggctggct cgcgcatcgg ccggccggcc
     1381 tgccgaagat cagcgcctgg gaaacggcgc cgggagacgt cgtccggccg ccgaagggca
     1441 cgacgcatgt cgtcctcgat acgccggccg gcctgcatgg caagcgtctc gacgaggtca
     1501 tgaagctcgc cgacaaggtg ctgattccgc tgcagccgag catcttcgac atcaacgcca
     1561 cgcacgaatt catcggccag ttgctggcgc accggcgcag cccgcaggtg cagctggcgg
     1621 tggtgggcac gcgcacccgc gagggcacga tcgccaccga tcagctgcgt catttcctcg
     1681 agtcgatgcg cgtgccgctg ctcgcattcc tgcgcgacac ccagaactac gtgcacctcg
     1741 ccgcgcacgg tctgaccttg tgggatgtgt cgtcggtacg cttcgagcgc gaccgcgagc
     1801 agtgggagcc gatcatcgtc tgggtcaatt gagaacgacg ggaagcgaga tgagaacgtt
     1861 cgccacgctg gccgagttgc agcatctcgt gggccaggag ctcggggtca gcgactggat
     1921 cacggtgacg caggagcgca tccagctctt cgccgacgcg accggcgacc accagtggat
     1981 ccacctcgat cgcgaacgcg ccaaggcggg tccgttcgga acgacgatcg cgcatggctt
     2041 cctgacgctg tcgctgctgc ccgagatggg cgccaaggcg ttcgcggtcg ccgacacgaa
     2101 gatgggcgtc aactacgggc tcaaccgggt ccgcttcccg gcgccggtgc cgagcggcag
     2161 tcgcatccgc ggacgcttca cgctgtcgaa gttcgaggcg gtcgacggcg gcgcgcagct
     2221 cgtcatcgag gtgaagatgg agcgcgaagg ctccgacaag cccgtctgcg tcgccgaatc
     2281 ggtgtcgcgg cgatacacat gactcggcac gaccgcgaat cgactgcacc atgaaggttc
     2341 ttctcatcga cgatcacccg ctgatcctga cggcgttgca gaacgtcatc cagggcatcg
     2401 gcagccacgt ctcggtcgtc ggcgtggccg gggcgcgcgg cgcccgc
//
LOCUS       DASRWS010000562         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2499010, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000562 DASRWS010000000
VERSION     DASRWS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499010"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2475
                     /gene="pepN"
                     /locus_tag="VFF72_10695"
     CDS             <1..2475
                     /gene="pepN"
                     /locus_tag="VFF72_10695"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HZV93675.1"
                     /translation="NGEPVSFRHEDGLLVIDNVPEGSFALEIRNTCAPEKNTQLSGLY
                     TSGGGFFTQCEAEGFRRITYFLDRPDVMAEYTVLIRANKAAYPVLLSNGNLVEQGDLD
                     GGRHFARWHDPFPKPSYLFALVAANLVAREQHVRSRSGKDHLLQVWVRRGDLDKTEHA
                     MNSLIASVVWDEARFGLTLDLERFMIVAVGDFNMGAMENKGLNLFNTKYVLANPATAT
                     DDDYEAIESVIGHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFSMDMLATATARA
                     KKRIEDVRRLRQIQFSEDAGPMAHPVRPDSYVEINNFYTATVYEKGAEVVRMMQTLVG
                     RDGFRRGLALYFERHDGQAVTCDDFAQAIADANPESVLARRLAQFKRWYAQAGTPKVT
                     ARGRYDAPSRTYTLGLEQAPAAAAGEGAGPLVIPLAIGLVGRDGQALPLRLEDEPADS
                     ADRERLLVLDEPRAFYTFVDVDQPPVLSLLRGFSAPVCLVDGLGDAELLVLLRHDADP
                     FNRWEAGQRLALARLVAAANGEAAATVPDDEFVAAMREVLRHPTLDASFKALVLTLPS
                     EAYVAEHVESVDPQRIHAGREALRHRLATELFDDWQEAFEANQLGGGYSPDAASAGHR
                     ALAGLALTMLCVASVDRGDAVWPGRAYQRVKDASNMTEREAGLLALIVSGSPLAEAAL
                     ARLHDLFHDDPLVIDKWFSVQATAPERDGRVFERVRRLLAHPDFSLTNPNRARSLIGA
                     FCNGNPAGFHRADGSGYAFWADRVIELDPINPQLAARIARALDRWARLAEPYRAAARS
                     ALGRVAAHPGLSSDTAEIVTRALAER"
     gene            2556..3824
                     /locus_tag="VFF72_10700"
     CDS             2556..3824
                     /locus_tag="VFF72_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZV93676.1"
                     /translation="MASRAIRKNAFYAQSGGVTAVINASACGVIETARGHRDRIGTVF
                     AGRNGIIGALTEDLIDTRRETAAAIRALRSTPAGAFGSCRYKLKSLEQNRREYERLIE
                     VFRAHDIGWFFYNGGGDSADTCFKVSQLSKTMGYPLQAIHIPKTVDNDLPVTDCCPGF
                     GSVAKYVAVSTLEASLDVRSMARTSTKVFVLEVMGRHAGWIAAAGGLVESHGIPVVIL
                     FPEIEFDEKRFLARVDALVESHGYCTVVVSEGAHRADGRFLAEQGSRDAFGHAQLGGA
                     APVVAGMIKETLGHKFHWAVADYLQRAARHIASKTDVDQAYAVGRRAVELAIKGANAV
                     MPTIVRVADRPYRWKIGVADLADVANVEKFMPRDFITADGFGITEKCHRYLAPLIAGE
                     DYPAYRNGLPVYATLKNAAVAKKLPAFTLA"
     gene            complement(3857..4102)
                     /locus_tag="VFF72_10705"
     CDS             complement(3857..4102)
                     /locus_tag="VFF72_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93677.1"
                     /translation="MADDVTKKDLQYVQAVLNKKIDDVAKALAAEKSRAIADCQEVDA
                     IVVRVRSDLDKRLDRLEGSVDKLTKAMGELARRVAAS"
BASE COUNT          664 a         1512 c         1369 g          641 t
ORIGIN      
        1 aacggcgagc cggtctcgtt ccgccacgag gacgggctgc tcgtgatcga caacgtcccg
       61 gaaggcagct tcgcgctcga gatccgcaac acctgcgcgc ccgagaagaa cacccagctc
      121 tcgggcctct acacgtctgg cggcggcttc ttcacccagt gcgaggccga agggtttcgc
      181 cgcatcacgt atttcctcga ccggcccgac gtgatggccg aatacaccgt gctgatccgc
      241 gccaacaagg ccgcgtatcc ggtgctgctc tcgaacggca acctcgtcga acagggcgac
      301 ctcgatggcg gacgccactt cgcccgctgg cacgatccgt tcccgaaacc gagctacctc
      361 ttcgccctcg tcgccgccaa cctggtcgcc cgcgagcagc acgtccgctc acgttcgggc
      421 aaggatcacc tgctccaggt ctgggtgcgc cgcggcgacc tcgacaagac cgagcacgcg
      481 atgaactcgc tgatcgcgtc ggtggtctgg gacgaggcgc gattcggcct gacgctcgat
      541 ctcgagcgct tcatgatcgt cgccgtcggc gacttcaaca tgggcgcgat ggagaacaag
      601 gggctcaacc tcttcaacac caaatacgtc ctcgccaacc cggccaccgc caccgacgac
      661 gactacgaag cgatcgaatc ggtgatcggc cacgagtact tccacaactg gacgggcaat
      721 cgcatcacct gtcgcgactg gttccagctg agcctgaaag aaggcctgac ggtctttcgc
      781 gaccaggaat tcagcatgga catgctggcc acggcgacgg cacgcgcgaa gaagcggatc
      841 gaggacgtac gacggttgcg ccagatccag ttctcggaag acgcggggcc gatggcccat
      901 ccggtgcggc ccgacagcta cgtcgagatc aacaacttct acaccgccac cgtctacgaa
      961 aagggcgccg aggtcgtgcg catgatgcag acgctggtcg gccgcgacgg cttccggcgc
     1021 ggcctggcgc tgtatttcga aaggcacgac gggcaggccg tcacctgcga cgacttcgcg
     1081 caggcgatcg ccgacgccaa cccggagagc gtcctggcgc gccgcctcgc gcagttcaag
     1141 cgctggtacg cgcaggccgg cacgccgaag gtcacggcgc gcggccgcta cgacgcgcca
     1201 tcgcgcacct acacgctcgg cctcgagcag gcgccggcgg cggccgccgg cgagggcgcc
     1261 gggccgctgg tgatcccgct ggcgatcggc ctcgtcggcc gcgacggaca ggcgctgccg
     1321 cttcgtctcg aagacgagcc ggccgactcc gccgaccgcg agcggcttct tgtgctcgac
     1381 gagccccgcg ccttctacac cttcgtcgac gtcgaccagc cgccggtgct ctcgctgttg
     1441 cgcggcttct cggcgccggt ctgcctcgtc gacggcctgg gcgacgccga gctgctggtg
     1501 ctgctgcgcc acgacgccga tccgttcaat cgctgggagg ccggccagcg gctcgccctc
     1561 gcccgcctcg tcgccgcggc gaacggcgag gccgccgcga ccgtgcccga cgacgaattc
     1621 gtcgccgcga tgcgcgaggt gcttcgccac ccgaccctcg acgcttcctt caaggcgctg
     1681 gtcttgaccc tgccgagcga ggcctacgtc gccgagcacg tcgaaagcgt cgatccgcag
     1741 cgcatccacg ccggccgcga agccctgcgc caccgcctgg ccaccgagct cttcgacgac
     1801 tggcaagagg cgttcgaggc gaaccagctc ggcggcggct attcgcccga tgcggcgtcg
     1861 gccggacatc gcgcgctcgc cggcctcgca ttgacgatgc tctgcgtcgc cagcgtggat
     1921 cgcggcgacg ccgtgtggcc cggccgcgcc tatcagcgcg tcaaggacgc gtcgaacatg
     1981 accgagcgag aggccggctt gctcgcactc atcgtcagcg gctcgccgct cgcagaggcg
     2041 gcgctggcgc ggctgcacga cctgttccac gacgatccgc tggtcatcga caagtggttc
     2101 tcggtgcagg cgacggcgcc cgagcgcgac ggccgcgtct tcgagcgagt ccgtcgactg
     2161 ctcgcgcacc cggacttcag cctgaccaat ccgaaccgcg cgcgcagcct gatcggcgcc
     2221 ttctgcaacg gcaatccagc gggctttcat cgcgccgacg gcagcggcta cgcgttctgg
     2281 gccgatcgcg tcatcgagct cgatccgatc aacccgcaac tcgccgcaag aatcgcccgc
     2341 gccctcgatc gctgggcccg cctcgccgag ccgtaccggg ccgcggcgcg gtcggcactc
     2401 gggcgggtcg ccgcgcatcc gggactgtcg agcgacaccg ccgagatcgt cacgcgggcg
     2461 ctggcggagc gctaggatga cgacgcatcc gcaggctcgc cggccctcgc cttcgcgaac
     2521 catgcgccgt cttcaaatga tcggagggca atgacgtggc cagccgcgcg atccgcaaga
     2581 acgcgttcta tgcccagtcg ggcggcgtca ccgccgtcat caacgcgtcg gcgtgcggcg
     2641 tcatcgagac ggcgcggggc catcgcgacc ggatcggcac ggtcttcgcc ggccgcaacg
     2701 gcatcatcgg cgcgctcacc gaagacctga tcgacacccg cagggaaacg gcagcggcga
     2761 tccgcgccct gcgctcgact ccggcgggcg ccttcggctc gtgccgctac aagctgaagt
     2821 cgctcgagca gaaccggcgc gagtacgagc ggctgatcga ggtctttcgc gcccacgaca
     2881 tcggctggtt cttctacaac ggcggcggcg actcggccga cacctgcttc aaggtgagcc
     2941 agctgtcgaa gacgatgggc tatccgctgc aggcgatcca catcccgaag acggtcgaca
     3001 acgacctgcc cgtcaccgat tgctgcccgg gcttcggctc ggtcgccaag tacgtcgccg
     3061 tctcgacgct cgaggcgtcg ctcgacgtgc gctcgatggc gcgcacctcg accaaggtct
     3121 tcgtcctcga ggtgatgggc cggcatgccg gctggatcgc ggccgccggc ggcctcgtcg
     3181 agtcgcacgg gatcccggtg gtcatcctct ttcccgagat cgagttcgac gagaagcgct
     3241 tcctcgcccg ggtcgatgcg ctcgtcgagt cgcacggcta ctgcaccgtg gtcgtttccg
     3301 aaggagcgca ccgcgccgac ggccgctttc tcgccgagca gggcagccgc gacgccttcg
     3361 ggcacgccca gctcggcggc gccgcaccgg tcgtcgcggg catgatcaag gagacgctcg
     3421 gccacaagtt ccactgggcg gtggccgact acctgcagcg cgcggcgcgg cacatcgcat
     3481 cgaagaccga cgtcgatcag gcctacgccg tcggcaggcg tgccgtcgag ctcgcgatca
     3541 agggcgccaa cgcggtgatg ccgacgatcg tgcgcgtggc cgaccgtccc taccgctgga
     3601 agatcggcgt cgccgacctg gccgacgtcg ccaacgtcga aaagttcatg ccccgcgact
     3661 tcatcaccgc ggacggcttc ggcatcaccg agaaatgcca tcgctacctg gcgcccctga
     3721 tcgccggcga ggactatccc gcctaccgga acgggctgcc cgtctacgcg acgttgaaaa
     3781 acgccgccgt ggcgaagaag ctacctgcct tcacgctggc ctgatcgaag gcgacgacgc
     3841 gaccgggtcg gtcgcgtcac gaggctgcga cgcgacgtgc cagctcgccc atcgccttgg
     3901 tgagcttgtc gacggagcct tcgagccgat cgaggcgctt gtccagatcc gagcgaacgc
     3961 gcacgacgat ggcatcgacc tcctggcagt cggcgatcgc gcggctcttt tcggccgcca
     4021 gggccttggc gacgtcgtcg atcttcttgt tgaggacggc ctgcacgtat tgcagatcct
     4081 tcttcgtcac atcgtcggcc atggcggtgt accggggggt cgcgaaggct ccattgtgtc
     4141 gtgctcgatc aacccgctgg cggcgtgcgc cggggcgccc cggcgg
//
LOCUS       DASRWS010000563         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3101023, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000563 DASRWS010000000
VERSION     DASRWS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101023"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1660)
                     /locus_tag="VFF72_10710"
     CDS             complement(11..1660)
                     /locus_tag="VFF72_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZV93678.1"
                     /translation="MDQTDTTQRSILLVVLPYRLGDATTDTTKKKIRSFLAFPYGVLT
                     LASYAQRYAQRLRGVEVLDLNLPTGESPDAILSRKLAETQPDIVGFSMSYDVSYQWLR
                     SLSEIVKRHDETTCVVAGGPAITTAYAEILDGCPHLDAACYSEGEVGLKNLIEADDFA
                     AVLAQDPWVTREKLARKTTAHPVYDDLDKIIDVDYSLVEIGAYSMKEAFSPFTRFVES
                     SKQFFIVTSRGCPFKCVFCAEPSFHGANMRYASVDRVVEHVARLKHQYGLGVLTIYDD
                     QILMNRARAKELFRKLAPLKIRIEMPNGVTLSYIDEEMAGLMKAAGVDTIFLAIEHGS
                     KRVLKEIIKKPIAFSRIKPTVQMLQNAGIFACTFLVIGLPGETRAEREETRAAVYDWG
                     VDWAFFNYATPLRGSELFRMCRDNVWIEEKYLPIGAIDMTDYVIRAPGMDAAEIKGTV
                     FEMNLDLNFVNNSNLRRGNLTTAYRSFREVVSRHPGQPFAHYFLAQCMEEMGVGQARI
                     DKHYQAYRDIIAGSDTWRAAADKFGLAPDPQAGMREERRRA"
     gene            complement(1988..2164)
                     /locus_tag="VFF72_10715"
     CDS             complement(1988..2164)
                     /locus_tag="VFF72_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93679.1"
                     /translation="MSEVYAPVARVMVVAALLAAITTLMLVTVQTARAAGALAIGSCG
                     AFGEAYDFRNPGDA"
     gene            complement(2232..>2540)
                     /locus_tag="VFF72_10720"
     CDS             complement(2232..>2540)
                     /locus_tag="VFF72_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZV93680.1"
                     /translation="VCLGDMPQVDATLIDKLIAAFDPEKGALVVVPSIDGRRGNPVVW
                     SRRFFPDLMAVQGDIGARHLIGSYAEAVAEVPVKGAAALTDVDTPESLSAVKAEIERI
                     "
BASE COUNT          426 a          817 c          852 g          445 t
ORIGIN      
        1 attccggcgc ctaggcgcgc cggcgctcct cgcgcatgcc ggcctgcgga tcgggcgcga
       61 gcccgaactt gtcggccgcc gcgcgccagg tgtcggagcc ggcgatgatg tcgcgatagg
      121 cttggtagtg cttgtcgatc ctcgcctgcc cgacgcccat ttcctccatg cattgcgcga
      181 ggaaatagtg cgcgaagggc tgacccggat gacgcgatac gacctcgcgg aacgagcgat
      241 aggcggtggt gagattgccg cggcgcaaat tgctgttgtt gacgaaattg agatcgagat
      301 tcatctcgaa cacggtccct ttgatctcgg ccgcatccat gcccggcgcg cgaatgacgt
      361 aatcagtcat gtcgatggcg ccaatcggca ggtacttctc ctcgatccag acattgtccc
      421 tgcacatgcg gaacagttcg ctgccgcgca gcggcgtcgc gtagttgaag aaggcccagt
      481 cgacgcccca gtcgtagacc gcggcacgcg tctcctcgcg ctcggcgcgc gtctcgcccg
      541 gcaggccgat gaccaggaag gtgcaggcga agatgccggc gttctggagc atctgcaccg
      601 tcggcttgat gcggctgaag gcgatcggct tcttgatgat ctctttgagc acgcgcttcg
      661 agccgtgctc gatggcgagg aagatggtgt cgacgcccgc ggccttcatc aggccggcca
      721 tctcctcgtc gatataggag agcgtgacgc cgttcggcat ctcgatgcgg atcttcagcg
      781 gcgcgagctt gcggaacaac tccttcgccc gcgcgcggtt catcaggatc tggtcgtcgt
      841 agatcgtcag cacgccgaga ccgtattggt gcttcaggcg cgctacgtgc tcgaccacgc
      901 ggtcgacgct cgcataacgc atgttggcgc cgtggaacga cggctcggcg caaaacacgc
      961 atttgaacgg gcagccacgc gaggtgacga tgaagaactg cttcgacgac tcgacgaagc
     1021 gcgtgaaggg cgagaacgcc tccttcatgc tgtaggcgcc gatctcgacc agcgagtaat
     1081 cgacgtcgat gatcttgtcc aaatcgtcgt agacgggatg cgcggtcgtc ttgcgcgcca
     1141 gcttctcgcg cgtcacccac ggatcctgcg ccagcaccgc ggcgaaatcg tcggcctcaa
     1201 tcaggttctt caagcccact tcgccctccg aatagcaggc ggcgtcgaga tgcgggcagc
     1261 cgtcgaggat ttcggcgtag gcggtggtga tcgccgggcc gccggcgaca acgcaggtgg
     1321 tctcgtcgtg gcgcttcacg atctcgctga gcgagcgcag ccactggtag gagacgtcgt
     1381 aggacatcga gaagccgaca atgtccggct gcgtttcggc gagcttgcgc gagaggatcg
     1441 cgtcggggct ttcgccggtc ggcagattga ggtcgagcac ctcgacgccg cgcagccgtt
     1501 gcgcgtaccg ctgcgcgtag gaggcgagcg tgagcacgcc gtaaggaaac gcgaggaagc
     1561 tcctgatctt cttcttggtc gtatcggtcg ttgcgtcgcc gaggcgatac ggcaggacga
     1621 ccaggaggat cgagcgctgc gttgtgtcgg tctggtccac gtccggtccc ccgctggccc
     1681 gcgcgcccgg cgcgtgagcc tagccaaccg aatacgtgcg cattcttaag gttgccttaa
     1741 ccatgcgcct gagaccgccc gcctagcctt tggcgtcgca gaaaaaggtg cggatgacgc
     1801 actccttggc gccgtcggcg tagcaatgct tgagcgcgtc gttctgggcg gcgccgagcc
     1861 gcggcccctt gccgacgcca tgcccgccgc aggcattggc gtagtcgacg gcgagcgccg
     1921 cgcagccgcg cttgaccgtg gcgacgacgc ggcaggtgcc gtccttgcat ttggcgagcg
     1981 cgctcttcta cgcgtcaccg ggattgcgga agtcgtaggc ctcgccaaag gcgccgcagg
     2041 agccgatcgc cagcgccccc gccgcccgcg cggtctgcac ggtgacgagc atgagcgtcg
     2101 tgatcgcagc cagcaaggcg gcgaccacca tcacgcgggc gaccggtgcg tagacctcgc
     2161 tcatgagagc cttaccccaa cttgcccgga acctggggcg agtcggggac ggcgatcaat
     2221 gattcgaatc tctagatgcg ctcgatttcg gccttgaccg cggacagcga ctcgggggta
     2281 tcgacgtcgg tcagggcggc cgcgcccttg accggcacct cggcgaccgc ctcggcgtag
     2341 ctgccgatca ggtggcgggc gccgatgtcg ccctgcaccg ccatcaggtc ggggaagaag
     2401 cgccgcgacc acacgaccgg attgccgcgg cgcccgtcga tcgacggcac cacgaccagc
     2461 gcccccttct ccggatcgaa ggcggcgatg agcttgtcga tcagagtggc gtcgacctgc
     2521 ggcatgtcgc cgaggcagac
//
LOCUS       DASRWS010000564         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2500515, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000564 DASRWS010000000
VERSION     DASRWS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500515"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFF72_10725"
     CDS             <1..272
                     /locus_tag="VFF72_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93681.1"
                     /translation="QMRKFQQTQQPPHSLLAPINEAPVIPPIPRAVGPQAPLSAPQTS
                     PGTTPGPLQRQRALRPYPAASDTVYVPFPVPAAASAPAATPHPVN"
     gene            280..1701
                     /gene="gspE"
                     /locus_tag="VFF72_10730"
     CDS             280..1701
                     /gene="gspE"
                     /locus_tag="VFF72_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HZV93682.1"
                     /translation="MGARHPLPYAYAKANTLLLEDDGNRLVLWAPETVGLPALSEVLR
                     LYDVDAFERESADTLVGRIAAAYAGGESSAAVVIGEVESSVDLSRMMQELPAIEDLLE
                     ASNDAPIIRMLNALLTQAAKDGASDIHIEPYERSSSVRFRVDGTLREVVQPNKALHAA
                     LVSRLKIMAELDIAERRLPQDGRISLRIGGRPVDVRVSTLPSAHGERAVLRLLDKAES
                     KFTLEALGMSGDVLTRFDALIKQPHGIILVTGPTGSGKTTTLYASLGRVDTAGTNILT
                     VEDPVEYELAGIGQTQVNAKIDLTFAKALRAILRQDPDVIMIGEIRDYETAQIAIQAS
                     LTGHLVLATVHTNDAPSTVTRMIDMGVEPFLLSSSLIGVLAQRLVRKLCVHCRRADAE
                     GRFHPVGCEHCAMSGYKGRTGVYELMQVDDRIQSLIHGRAAEAEVFAAAEAAGLKSMR
                     EDGDRLVAEGVTSAEEVIRVTRE"
     gene            1793..>2155
                     /locus_tag="VFF72_10735"
     CDS             1793..>2155
                     /locus_tag="VFF72_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZV93683.1"
                     /translation="MPAFRFEALDALGKSTTGLLEADNARAARSQLRAQALVPLAVAQ
                     VASAGTATTGLRLGGRVFNSTSLAVWTRQIAGLVGSGLPLERALTALSDEAEDPKQRE
                     LVAHLRSEVNSGSPFAQAL"
BASE COUNT          340 a          779 c          729 g          307 t
ORIGIN      
        1 agcagatgcg caagttccag cagacgcagc agccgccgca cagcctcctg gcgccgatca
       61 acgaggcgcc cgtcattccg ccgatcccgc gcgcagtcgg cccgcaggcg cctctctcgg
      121 cgccgcagac ttcgccggga acgacgcccg gtcccctgca gcgccagagg gcgctccgcc
      181 cttatccggc ggcctccgac acggtctacg tgccgtttcc ggtgccggcg gcagcgtccg
      241 ctccggcggc gacgccgcat ccggtgaact gaccaggcca tgggcgcccg acatccgctg
      301 ccctacgcct acgccaaggc gaacacgctg ctgctcgagg acgacggcaa ccggctggtg
      361 ctgtgggcgc ccgagacggt cggtctgccg gcgctgtccg aagtgctgcg cctctacgac
      421 gtcgacgcgt tcgagcgcga gtcggccgac accctcgtcg gacgaatcgc cgctgcctac
      481 gccggcggcg aatcgagcgc cgccgtcgtc atcggcgagg tcgagagctc ggtggacctg
      541 tcgcggatga tgcaggaact gccggcgatc gaggacctgc tcgaggcgtc gaacgacgcg
      601 ccgatcatcc gcatgctcaa cgcgcttctc acgcaggcgg caaaggacgg cgccagcgac
      661 atccacatcg agccgtacga gcgttcgagc tcggtgcgct ttcgcgtcga cggcaccctg
      721 cgcgaagtcg tgcagccgaa caaggcgctg cacgcggcgc tggtctcgcg cctgaagatc
      781 atggccgagc tcgatatcgc cgagcgcagg ctgccgcagg acggccgcat ctcgctgcgc
      841 atcggcggcc ggccggtcga cgtgcgcgtc tccaccctgc cctcggcgca tggcgagcgg
      901 gccgtgctgc gactgcttga caaggccgag tcgaaattca cgctcgaggc cctcggcatg
      961 agcggcgacg tgctgacgcg cttcgacgcc ctcatcaagc agccgcacgg aatcatcctc
     1021 gtcaccgggc ccaccggctc gggcaagacg acgacgctct acgcctcgct cggccgcgtc
     1081 gacaccgcgg gaaccaacat cctgaccgtc gaggatccgg tcgaatacga gctcgcgggc
     1141 atcggccaga cgcaggtcaa tgccaagatc gacctgacgt tcgccaaggc gctgcgcgcc
     1201 atcctgcgcc aggaccccga cgtcatcatg atcggcgaga tccgcgacta cgagacggcg
     1261 cagatcgcga tccaggcgtc actcaccggc cacctcgtgc tcgccaccgt ccacaccaac
     1321 gacgcgccga gcaccgtgac gcggatgatc gacatgggcg tcgagccgtt cctgctgtcg
     1381 tcgagcctga tcggcgtgct cgcgcaacgc ctggtgcgaa agctctgcgt gcattgccgc
     1441 agggccgatg ccgaggggcg ctttcacccc gtcggctgcg agcactgcgc catgtcgggc
     1501 tacaagggac gcaccggcgt ctacgagttg atgcaggtcg acgacaggat ccagtcgctg
     1561 atccatggtc gtgccgccga ggccgaagtc ttcgctgccg cggaggcggc cggtctcaag
     1621 tcgatgcgcg aagacggcga ccggctcgtc gccgagggcg tcacctcggc cgaggaagtc
     1681 atccgggtga cgcgcgaatg agcctcgaca tcgcgtcggg cgatccggcc ggcagcgttt
     1741 ccgagttcgg aagccacgga ttgcgggccg gcgcttcggc tcggcgctga cgatgccggc
     1801 ctttcgcttc gaggcgctcg acgcgctcgg caagtcgacc accggcctgc tcgaagccga
     1861 caacgcacgc gcggcgcggt cgcagctgcg cgcccaggcg ctggtgccgc tggccgtcgc
     1921 gcaagtcgct tcggccggta cggcgacgac cgggctgcgg ctgggcggcc gggtattcaa
     1981 ctcgaccagc ctcgcggtat ggacccggca gatcgccggg ctcgtcggct cgggcctgcc
     2041 gctcgagcgg gcgctcacgg cgctcagcga cgaggccgaa gatccgaaac agcgcgagct
     2101 cgtcgcgcac ctgcgcagcg aagtcaattc aggctcgccg ttcgcgcagg cgctg
//
LOCUS       DASRWS010000565         6382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2501037, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000565 DASRWS010000000
VERSION     DASRWS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501037"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..824
                     /locus_tag="VFF72_10740"
     CDS             45..824
                     /locus_tag="VFF72_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZV93684.1"
                     /translation="MACFGAASQAAERVLTMASTTSTEQSGLFPHLLPAFTKATGIDV
                     HVVAVGTGQALDMGRRGDADVLFVHDKAAEEKFVAEGWGLPRRDVMYNDFVLVGPKDD
                     PAAVKGHDIVAALAKLWASPSVFISRGDRSGTNAAELRYWKAAGIDVPGQKMSGYREC
                     GCGMGPALNMASSINAYVLADRGTWLSFRNRGDLAILVEGDKRLFNQYGVIVVNPARH
                     PQTKTALAQAFSDWVVSPQGQATIASYRVGGEQLFFPDARP"
     gene            837..1385
                     /gene="mobB"
                     /locus_tag="VFF72_10745"
     CDS             837..1385
                     /gene="mobB"
                     /locus_tag="VFF72_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HZV93685.1"
                     /translation="MKVVGFCGYSGAGKTTLAEQLVAFLTRAGLRVSVVKHAHHDFDI
                     DHPGKDSWRHRQAGAYEVVIASDRRMAKMREYPRLEEPTVDALLGELVDCDWALVEGF
                     KHAALPKIEVWRAATGKPALYPADDHVIAVATDDVAALPASTALPLFELGEIERIAAF
                     LQGRGDRHEYRPGRFRVGVGVD"
     gene            complement(1470..1997)
                     /locus_tag="VFF72_10750"
     CDS             complement(1470..1997)
                     /locus_tag="VFF72_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HZV93686.1"
                     /translation="MRNATELLAQYAAYHRDRRNIVSHFVGVPMIVFGIGVLLARPAA
                     PAFGLSLTPAWVAFSLAAAWYLTRGNVVLGVAVSAAVGLLIKLGHEVAGGSVGVWLGW
                     GAGFFAFGWLIQFMGHWYEGRKPAFVDDVIGLLVGPMFVTAEAMFMLGWNKPLLDEIE
                     RRAGPTHLRDIARIA"
     gene            2158..2727
                     /gene="dcd"
                     /locus_tag="VFF72_10755"
     CDS             2158..2727
                     /gene="dcd"
                     /locus_tag="VFF72_10755"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZV93687.1"
                     /translation="MSIKSDKWIRRMAAEHAMIEPFEPGQVRHASDGHRIVSYGTSSY
                     GYDIRCAPEFKVFTNIFSTVVDPKNFDEKSFVDIQADVCVIPPNSFALARTVEYFRIP
                     RNVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVIF
                     FESDEVCETSYKDRGGKYQGQRGVTLPKT"
     gene            2808..3701
                     /locus_tag="VFF72_10760"
     CDS             2808..3701
                     /locus_tag="VFF72_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZV93688.1"
                     /translation="MRMDGEEESSNVEDVRGMGGGGGGGFGFGGRSIGLGSVVVALIA
                     GWIFGVNPLTLLGLAGGGGGSGGQVVQQQSRPAQAPPANDPQAKFVAQVLHSTEVVWT
                     DVFRESGRTYHPPRLVLFSGSWPTACGQGQAAMGPFYCPNDQKVYIDLSFYDLLSRKL
                     GAPGQFAQAYVIAHEVGHHVQNELGIMQKVDAMRGRGGEAQANALSVRVELQADCFAG
                     VWANRSQKEQGWRLEPGDIETALNAAAQIGDDTLQRKSRGTVVPETFTHGTSAQRVGW
                     FKRGFDKGSVDDCNTFRSRDL"
     gene            complement(3776..4939)
                     /locus_tag="VFF72_10765"
     CDS             complement(3776..4939)
                     /locus_tag="VFF72_10765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZV93689.1"
                     /translation="MTPTTTSPVITHIEDLREIARKRVPRMFYDYADTGSWTESTYRA
                     NEADFQKIKLRQRVAVDIEKRSLRSTMVGEDVAMPVALAPIGMLGMQHADGEILAARA
                     AEAFGVPFTLSTMSICSIEDVAANTTRPFWFQLYVMRDREFIGRLIDRAKAARCSALV
                     LTLDLQILGQRHKDIKNGLSTPPKPTLANLLNLATKPAWSMAMLGTKRRSFGNIVGHV
                     AGVRNMASLGAWTAQQFDPRLSWADIDWIRKRWGGKLILKGILDAEDAKLAADSGADA
                     LIVSNHGGRQLDGAPSSIAALPAIAEAVGSRIEVWMDGGIRSGQDILRARALGAQAVM
                     IGRAFVYGLGAMGQLGVTRALEVLANELELTMAFCGHTDIRKVDRDVLLPGTY"
     gene            complement(4978..6186)
                     /locus_tag="VFF72_10770"
     CDS             complement(4978..6186)
                     /locus_tag="VFF72_10770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZV93690.1"
                     /translation="MDAFYASVELLRYPELRGRAVVIGGGRDHQPRRRADGTHDFDVL
                     RDYVGRGVVTTSTYEARKLGVFSAMGMMKSAALAPEAILLPVDFDEYRRYSRLFKAAV
                     REIAPVVEDRGIDEIYIDLSELPGVDADGGRAAGLRMKASVREATGLTCSIGITPNKL
                     LSKIASELDKPDGLTLLEHADIPARIWPLAARKVNGIGPKAALKLAEMGLHTIGDIAA
                     ADPLVLVARFGRSFGAWLHEASHGRDERPVVTQSEPVSISRETTFERDLHAVRDRAEL
                     GAVFTDLCSKLGGDLSRKGYAARTIGIKLRFDDFTTATRAQTLPAHTLDGREIRKAAG
                     QCLKRVDLSRRLRLLGVRAAGLEKLSDLVARGALESRPRPARARRDEHLADELPLFAS
                     IGSPSGTKPA"
BASE COUNT          978 a         2179 c         2284 g          941 t
ORIGIN      
        1 gccgtccgtt cgcggcatgc ctggcgttcg catgcgcgct ttcgctggcc tgcttcggcg
       61 ctgcctcgca agccgccgag cgcgttctca cgatggcgtc gacgacgagc accgagcagt
      121 cgggcttgtt cccgcatctc ctgccggcgt tcacgaaggc gaccggcatc gacgtgcacg
      181 tcgtcgccgt cggtaccggt caggcgctcg acatgggccg tcgcggcgat gccgacgtcc
      241 tcttcgtgca cgacaaggcg gccgaggaga agttcgtcgc cgagggctgg ggcctgccgc
      301 gccgcgacgt catgtacaac gatttcgtcc tcgtcggccc gaaggacgat cccgcggccg
      361 tcaagggaca cgacatcgtc gccgccctcg cgaagctgtg ggcgtcgccg agcgtcttca
      421 tctcgcgcgg cgaccgcagc ggcaccaacg ccgccgagct gcgctactgg aaggcggccg
      481 gcatcgacgt gccgggccag aagatgagcg gctaccggga gtgcggctgc ggcatgggac
      541 cggcgctcaa catggcgtcg tcgatcaacg cctacgtcct cgccgatcgc ggcacctggc
      601 tcagcttcag gaaccgcggc gacctggcga tcctggtcga gggcgacaag cggctcttca
      661 accagtacgg cgtcatcgtc gtcaacccgg cccgccatcc ccagaccaag accgcgctgg
      721 cccaggcctt ctccgactgg gtcgtctcgc cgcagggcca ggcgacgatc gcctcgtacc
      781 gggtcggcgg cgaacagctc ttctttcccg atgccaggcc ctgagcgaac ccgtcggtga
      841 aggtcgtcgg tttctgcggc tattcgggcg ccggcaagac gacgcttgcc gagcagctcg
      901 tcgcgttcct gacgcgggcg ggtcttcgcg tctcggtcgt caagcacgct caccacgact
      961 tcgacatcga ccaccctggc aaggattcat ggcggcatcg ccaggccggc gcgtacgagg
     1021 tggtgatcgc gtccgaccgg cggatggcca agatgcgtga atatccgcgt ctcgaggagc
     1081 cgacggtcga cgcgctgctc ggcgagctcg tggattgcga ctgggcgctg gtcgaaggct
     1141 tcaagcacgc ggcgctgccg aagatcgaag tctggcgcgc cgccaccggc aagccggcgc
     1201 tgtatccggc cgacgatcac gtgatcgccg tcgccaccga cgacgtcgcg gcgctgcccg
     1261 cgtcgacggc cctgccgctg ttcgagctcg gcgaaatcga gcggatcgcg gcattcctgc
     1321 agggacgcgg cgatcgtcac gagtaccggc cgggacgctt tcgcgtcggc gtcggcgtcg
     1381 actagtcggc gcggacgacg cggacgacgc ggacgacgcg gacgacgcgg acgacgcgga
     1441 cgacgcggcc tcctgccgcg tcgggagaat caggcgatcc gggcgatgtc gcgcaggtgc
     1501 gtcggcccgg cgcgccgctc gatctcgtcg agcagcggct tgttccagcc gagcatgaac
     1561 atcgcctcgg cggtgacgaa catcggcccg acgagcaggc cgatgacatc gtccacgaag
     1621 gcgggcttcc tgccttcgta ccagtggccc atgaactgga tcagccagcc gaaggcgaag
     1681 aagccggcgc cccagccgag ccagacgccg acgctgccgc cggcgacctc gtggccgagc
     1741 ttgatgagca ggccgaccgc cgcgctgacg gcgacgccca ggaccacgtt gccgcgcgtc
     1801 aggtaccagg cggcggcaag cgagaaggcg acccacgcgg gggtgagcga gaggccgaag
     1861 gcgggcgccg cgggacgggc gaggagcacg ccgatgccga acacgatcat cggcacgccg
     1921 acgaaatggc tgacgatgtt gcgccggtcg cggtggtacg ccgcgtactg ggcgaggagc
     1981 tcggtggcgt ttctcatggg cgggcctcgg cggggcggtg ggggaatgtt aggccaactt
     2041 gcaagacccg tgcgcggtcg cggcggcgac gccgtgagcg accgcacggt gcgccgccgg
     2101 gtcgaggcgc gacggcggct ccctacaatc cgcgctcctt cctcggggcg agccggcatg
     2161 agcatcaaga gcgacaagtg gatccggcgc atggccgccg agcacgcgat gatcgagccg
     2221 ttcgagccgg ggcaggttcg ccacgccagc gacggccacc gcatcgtcag ctacggaacg
     2281 tcgagctacg gctacgacat ccgctgcgct cccgaattca aggtcttcac gaacatcttc
     2341 tcgaccgtcg tcgatccgaa gaacttcgac gagaaaagct tcgtcgacat ccaggccgac
     2401 gtctgcgtca tcccgccgaa cagcttcgcc ctggcgcgca ccgtcgagta cttccgcatc
     2461 ccgcgcaacg tgttgacgat ctgcctgggc aagagcacgt acgcgcgctg cgggatcatc
     2521 gtcaacgtga cgccgttcga gcccgagtgg gaaggctacg tgacgctgga gttcagcaac
     2581 acgacgccgc tgccggccaa gatctacgcc ggcgaggggt gcgcgcaggt gatcttcttc
     2641 gagagcgacg aggtctgcga gacgagctac aaggatcgcg gcggcaaata ccagggccag
     2701 cgcggcgtca cgctgcccaa gacctgacga cggcgcggcc gcttcgccga cagggcgatg
     2761 cggcagcgcc ggctaccatc gccgcggcga gacgcgggga gacagcgatg cggatggacg
     2821 gcgaggaaga gagcagcaac gtcgaggacg tgcgcggcat gggtggcggc ggcggaggcg
     2881 gcttcggctt cggcggccgc agcatcggcc tgggcagcgt cgtcgtcgcc ctgatcgccg
     2941 gctggatctt cggcgtcaat ccgctgacgc tcctcggcct ggccgggggc ggcggcggaa
     3001 gcggcggcca ggtcgtgcag cagcagtcac ggcccgcgca ggcgccgccg gccaacgatc
     3061 cgcaggcgaa gttcgtcgcc caggtcctgc acagcactga ggtggtctgg accgatgtct
     3121 ttcgcgagtc gggtcgcacc taccatccgc cgaggctcgt tctcttcagc ggcagctggc
     3181 cgaccgcctg cggccagggc caggcggcga tggggccgtt ctattgcccg aacgaccaga
     3241 aggtctacat cgacctgtcg ttctacgacc tcctgtcgcg caagctcggc gcgccgggtc
     3301 agttcgccca ggcctacgtg atcgcgcacg aggtcggcca tcacgtccag aacgagctcg
     3361 gcatcatgca gaaggtcgac gcgatgcgcg gccgcggtgg cgaggcacag gccaacgcgc
     3421 tcagcgtgcg ggtcgagctg caggccgact gcttcgccgg cgtctgggcc aaccgatcgc
     3481 aaaaggagca gggctggcgt ctcgaaccgg gcgacatcga gacggcgctc aacgccgccg
     3541 cgcagatcgg cgacgacacg ctgcagagga agtcgcgcgg cacggtggtg ccggagacct
     3601 tcacgcacgg caccagcgcc cagcgggtcg gctggttcaa gcgcggcttc gacaagggca
     3661 gcgtcgacga ctgcaacacc ttccgcagcc gcgacctctg accgtcgcct cgcgggcgcg
     3721 ggcgccggcg tcgcgacgcc ttttcgcgtg aggggcaggc gcgcttcgcg aagcgtcagt
     3781 acgtgccggg cagcagcacg tcgcggtcga ccttgcggat gtcggtatgg ccgcagaagg
     3841 ccatggtgag ctcgagctcg ttggcgagca cctcgagggc ccgcgtcacg ccgagctggc
     3901 ccatcgcgcc caggccgtag acgaaggccc ggccgatcat gacggcctgg gcgccgagcg
     3961 cgcgcgcccg gaggatgtcc tggcccgatc ggatgccgcc atccatccag acctcgatcc
     4021 gcgagccgac cgcctcggcg atcgccggca gcgccgcgat cgacgagggc gcgccatcga
     4081 gctgccggcc accgtggttc gagacgatca gcgcgtcggc gccgctgtcg gccgcgagct
     4141 ttgcgtcttc cgcgtcgagg atgcccttca ggatgagctt gccgccccag cgcttcctga
     4201 tccagtcgat gtcggcccac gagagccgcg ggtcgaactg ctgcgccgtc caggcgccga
     4261 gcgaggccat gttcctgacg ccggcgacat ggccgacgat gttgccgaag ctgcgccgtt
     4321 tcgtgccgag catcgccatg ctccaggccg gcttggtcgc caggttgagc aggttggcca
     4381 gggtcggctt cggcggtgtc gacaggccgt tcttgatgtc cttgtggcgc tggccgagga
     4441 tctgcaggtc gagcgtcagg accagggcgc tgcatcgcgc ggccttggcg cgatcgatca
     4501 gccggccgat gaactcgcgg tcgcgcatca cgtagagctg gaaccagaac ggccgggtgg
     4561 tgttggcggc gacgtcctcg atcgagcaga tgctcatcgt cgacagggtg aagggcacgc
     4621 cgaaggcctc ggcggcgcgc gccgccagga tctcgccgtc ggcgtgctgc atgccgagca
     4681 tgccgatcgg cgccagcgcc accggcatcg ccacgtcctc gcccaccatc gtcgagcgca
     4741 ggctccgctt ctcgatgtcg acggcgacgc gctggcgcag cttgatcttc tggaagtcgg
     4801 cctcgttggc gcgataggtg ctctccgtcc acgagccggt gtcggcgtag tcgtagaaca
     4861 tccgcggcac gcgcttcctg gcgatctcgc gcaggtcctc gatgtgggtg atgacaggag
     4921 aagtcgtcgt cggggtcatc ccgggatgct acgacagcgc tcgcggcgaa acggcgctca
     4981 cgccggcttc gttccggacg gcgagccgat cgatgcgaag agcggcagct cgtcggcgag
     5041 atgctcgtcg cgtctcgccc tggccgggcg cggccgcgat tcgagcgcgc cgcgggcgac
     5101 gaggtcgctc agcttctcga ggccggcggc gcgaacgccg agcaggcgca ggcgccgcga
     5161 caggtcgacc cgcttcaggc actggccggc cgccttcctg atctcgcggc cgtcgagggt
     5221 gtgcgcgggc agcgtctgcg cccgcgtggc ggtcgtgaaa tcgtcgaagc gcagcttgat
     5281 gccgatggtc cgcgcggcat agccctttct cgacaggtcg ccaccgagct tgctgcacag
     5341 gtcggtgaag acggcaccga gctcggcccg gtcgcgaacc gcgtgcaggt cgcgctcgaa
     5401 ggtcgtctcg cggctgatcg agacgggctc gctctgcgtc acgacgggtc gttcgtcgcg
     5461 gccgtgcgaa gcctcgtgca gccaggcgcc gaagctgcgg ccgaagcggg cgacgaggac
     5521 gagcggatcg gcggcggcga tgtcgccgat cgtgtgcagg cccatctcgg cgagcttcag
     5581 cgccgccttc gggccgatgc cgttgacctt gcgcgcggcg agcggccaga tccgcgccgg
     5641 aatgtcggca tgctcgagca gcgtcaggcc gtcgggcttg tcgagctcgg aggcgatctt
     5701 cgagagcagc ttgttgggcg tgatgccgat cgagcaggtg aggccggtgg cctcgcgcac
     5761 gctcgccttc atgcgcagcc cggcggcgcg gccgccgtcg gcgtcgacgc cgggaagctc
     5821 gctcaggtcg atgtagatct cgtcgatgcc gcggtcctcg acgaccggcg cgatctcgcg
     5881 caccgccgcc ttgaacaggc gcgagtagcg gcggtactcg tcgaagtcga ccggcagcag
     5941 gatcgcctcg ggcgcgagcg ccgccgactt catcatcccc atcgccgaga agacgccgag
     6001 ctttcgtgcc tcgtacgtgc tcgtcgtgac gacgccgcgg ccgacgtagt cgcgcaggac
     6061 gtcgaagtcg tgcgtgccgt cggcgcggcg acgcggctgg tggtcgcgtc cgccgccgat
     6121 gacgacggcg cgcccgcgca gctcgggata gcgcagcagc tcgaccgacg cgtagaacgc
     6181 gtccatgtcg agatgcgcga ccaggcgtgg cacgacagga ctgtacgtgc atacagcgtc
     6241 gcacggcaag cgcggccgcc gcgtgcggcg cccctgcttg ccgcagaatg cgccgcatga
     6301 gcagcgcctc gccggccgcc tcgatccgct tcgcgacgcg cgccgacgcg ctcgtcatcg
     6361 ccgagatgtc gcgcgactac at
//
LOCUS       DASRWS010000566         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1932892, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000566 DASRWS010000000
VERSION     DASRWS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932892"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1153)
                     /locus_tag="VFF72_10775"
     CDS             complement(68..1153)
                     /locus_tag="VFF72_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZV93691.1"
                     /translation="MGEVTDLAAAAAAVDLDGPAPATVDGPPTAALIEALDRDGAVRQ
                     AWRVARWPVTLGRALDNDVVLTDPHVAAHHASIDHDANGSVVVRAGQTRNGLTIGRER
                     VEGGASRRFADPGRDLELHLGRTALRLRLAGHALAPEQVLAPRVALERRWAPTLALAV
                     AVLAIVLFNTWLDNDPDNLSRAIGSALLTAITGGAIWCGFWALLSKLFARQSNFGWHV
                     RVFVIASLVMLALSALPPLLEFSLSWPWLTDFSFVAAFATMAGAVYFHLLAVEPGRRR
                     LMRGVAVTGFAVGVALTLWFNVQRTGRPGEELYMNHLFPPALRLARPVSVDHFVDGMA
                     PMQSILDRQARERNGSEDDAASGDDDE"
     gene            complement(1141..2544)
                     /locus_tag="VFF72_10780"
     CDS             complement(1141..2544)
                     /locus_tag="VFF72_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZV93692.1"
                     /translation="MHSRRPFLLAACIVAGALSMPAWAVQMPPLPPLGPTAATPATVS
                     PAAPSEPSAASVPPALPAPVSLSARKVYEQARSQLVQIRTVLKGRASQTSVGSGFFVD
                     KGGLIVTNFHVVSEAALKPERHDLVYVTADGREAPLQILQLDVRHDLALLRAADAPSR
                     TFDALAFRTDAEPLAQGERIYSLGNPLDVGFAVVQGTYNGLVRRSFYPQIFFGGALSA
                     GMSGGPALDEEGHVIGINVARRVDGEQVSFLVPASFAVSLLAAGRDAKPLSGAAYAIV
                     DAQLMQHQQALTDRFIAQGWKKATHPRYHVPVPPDVFMRCWGTSAPSRTGGLDFERSD
                     CVMDTRIFVGDFTTGTIGLRHESYDGSRLGPLRFAARYAQSFRNESFVRLSSQYQTKP
                     RCNEDFVERAGLPLRAVVCMRAYKKLPQLYDVSVLVATLDQSVAGVQGRFDAQGVSFA
                     NAQRLTRHYLDAYGWAK"
     gene            2683..3708
                     /locus_tag="VFF72_10785"
     CDS             2683..3708
                     /locus_tag="VFF72_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93693.1"
                     /translation="MHRRTLLSASAAALALPSFAFGQALEKPKVALAVGGKNLLYYLP
                     LTIAETQGYFKDEGLDLTISDFAGGSRALQALVGGSADVVSGAFEHTITMQVKGQRLR
                     AFVLEGRAPQIVLGINPKTMPDYKTVADLKGKKLGVTAPGSSTQVLANFVLAKAGLKP
                     SDVSIVGVGAGNGAVAAIRSGQIDAISNLDPVITLLVRSGDLKIISDTRVVSVADRVF
                     GGPMPAACLYTHQTFIDKYPKTVQALTNAIVRADRWIQKAGPSDIVKAVPENYLLGDR
                     AVYVDAFLAAKGALSPDGMIPEAGAETARRALASIDPEIAAAKVDLSAIWTNEFVRRA
                     DAKYAKA"
     gene            complement(3736..4032)
                     /locus_tag="VFF72_10790"
     CDS             complement(3736..4032)
                     /locus_tag="VFF72_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93694.1"
                     /translation="MKHFATTVALAVVALSAGLPGVSIAGDTASVATPCTAPASMDHL
                     QRRIVAKASQGVDSLRDFIYITRGIYALDMNETVAWLDAQRRCVAVADAGDDGR"
     gene            4287..>4935
                     /locus_tag="VFF72_10795"
     CDS             4287..>4935
                     /locus_tag="VFF72_10795"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93695.1"
                     /translation="MSAVPALAFDKVVCTFVSKDDPGQRYTAVSDVTLSVGPGEFVSV
                     VGPTGCGKSTLLNVAAGLLAPSGGSVEVFGEPLRGINRRAGYMFQSESLMPWRTAIGN
                     VAAGLEFRGVGDAAGRAAEWLRRVGLGGFADRYPHQLSGGMRKRVSLAQTLVLDPDII
                     LMDEPFSALDVQTRQLMENELLALWAQKRKAVLFITHDLDEAIALSDRVVVLSAGP"
BASE COUNT          762 a         1686 c         1783 g          704 t
ORIGIN      
        1 ccgcgccgtc gccgcgccga tgccgcggcc gccaccggtg accagcaccg tgcgcatggc
       61 tggcgattca ctcatcgtcg tcccccgacg ctgcgtcgtc ttcgctgccg ttgcgctcgc
      121 gggcctgccg gtcgaggatg gactgcatcg gcgccatgcc gtcgacgaaa tgatcgacag
      181 acaccgggcg cgccaggcgc agcgccggcg gaaacaggtg gttcatgtag agctcctcgc
      241 cgggccgccc ggtgcgttgc acgttgaacc acagcgtcag cgcgacgccg accgcgaaac
      301 cggtgacggc gacgccgcgc atcagtcgcc tgcggccggg ctcgacggca agcaggtgga
      361 agtagaccgc gccggccatc gtggcgaagg cggcgacgaa gctgaagtcg gtcagccacg
      421 gccacgagag agagaactcg agcagcggcg gcagcgcgga gagggccagc atgacgaggc
      481 tcgcgatgac gaagacccgg acgtgccagc cgaagttgct ctgccgcgcg aagagctttg
      541 acagcaacgc ccagaagccg caccagatcg cgccgccggt gatcgccgtc agcagcgcgc
      601 tgccgatggc gcgactgagg ttgtcgggat cgttgtcgag ccaggtgttg aagagcacga
      661 tcgccagcac cgcgacggcc agcgccaggg tcggtgccca ccgccgctcg agggcgaccc
      721 tcggcgccag cacctgctcc ggggccagcg cgtggccggc gaggcgaagg cgcagcgcgg
      781 ttcgcccgag atgcagctcg aggtcgcgac ccggatcggc gaagcgccgg gacgccccgc
      841 cttcgacgcg ctcgcggccg atcgtcaggc cgttgcgggt ctggccggcg cgaacgacga
      901 cgctgccgtt ggcgtcatgg tcgatcgatg cgtggtgcgc ggcgacgtgc gggtcggtga
      961 ggacgacgtc gttgtcgaga gcccggccga gcgtcaccgg ccagcgggcg acgcgccagg
     1021 cctggcgaac ggcgccgtcg cgatcgagcg cttcgatcag tgccgcggtg ggcgggccgt
     1081 cgacagtcgc cggcgccggg ccgtcgagat cgacggcagc cgcggccgcg gcgaggtcgg
     1141 tcacttcgcc cacccgtagg cgtcgaggta gtggcgggtc agccgctgcg cgttggcgaa
     1201 gctcacgccc tgggcatcga agcggccctg cacgccggcc acgctctggt cgagcgtggc
     1261 gacgagcacc gagacgtcgt agagttgcgg cagcttcttg taggcgcgca tgcagaccac
     1321 ggcgcgcagc ggcaggccgg cgcgttcgac gaagtcctcg ttgcagcgcg gcttggtctg
     1381 gtactgcgac gacagccgca cgaagctctc gttgcgaaag ctctgcgcgt agcgggccgc
     1441 gaagcgcaac gggccgagcc gcgagccgtc gtagctttca tggcgcaggc cgatcgttcc
     1501 ggtcgtgaag tcgccgacga agatgcgcgt atccatgacg cagtcggagc gctcgaagtc
     1561 gaggccgccg gtgcgcgagg gcgcgctcgt gccccagcag cgcatgaaga cgtcgggcgg
     1621 caccggcacg tggtagcgcg gatgcgtcgc cttcttccag ccctgggcga tgaagcggtc
     1681 ggtgagcgcc tgctgatgct gcatcagttg cgcatcgacg atcgcatagg ccgccccgct
     1741 gagcggcttg gcgtcgcgtc cggcggccag cagggatacc gcgaagctcg ccggcaccag
     1801 gaagctgacc tgctcgccgt cgacccgacg cgcgacgttg atgccgatca cgtgcccttc
     1861 ctcgtcgagc gccgggccgc cgctcattcc ggcagaaagc gcgccgccga agaagatctg
     1921 cggatagaag ctgcgccgca ccagtccgtt gtaggtgccc tggacgacgg cgaagccgac
     1981 gtcgagcgga ttgccgagcg aatagatccg ctcgccctgc gccagcggct cggcgtcggt
     2041 gcgaaaggcc agggcgtcga aggtgcgcga gggtgcgtcg gcggcgcgca gcagggccag
     2101 gtcgtggcgc acgtcgagct gcaggatctg cagcggcgcc tcgcggccgt cggcggtgac
     2161 atagacaagg tcgtgtcgct ccggcttcag cgccgcttcg ctgacgacgt ggaaattggt
     2221 gacgatgagg ccgcccttgt cgacgaagaa cccggagccg accgacgtct ggctggcgcg
     2281 ccccttcagc acggtgcgga tctgcacgag ctgcgaccgc gcctgctcgt agaccttgcg
     2341 cgccgacagc gagacgggcg ccggcagcgc tggcgggacg gatgcggcgg acggctccga
     2401 aggcgcggcc ggcgaaacgg tggccggcgt cgctgccgtc ggaccgagcg gcgggagcgg
     2461 cggcatctgc acggcccagg ccggcatcga cagcgccccg gccacgatgc aggccgcgag
     2521 caggaagggc cgccgggaat gcatcgggcg gcattctacg tagccgccgt ccgccccggc
     2581 gctgaccagg ccgcggtcga acccgtcgca ggccgcaacg gcatcgcacg gtgcgcgagg
     2641 cttcgctaaa gtcgctgacc tgatcaatcc ggagaccgtt caatgcatcg ccgcaccctc
     2701 ttgtcggcga gcgccgccgc gctcgccctg ccgtccttcg ctttcggcca ggcgctcgag
     2761 aagcccaagg tcgcgctggc cgtcggcgga aagaaccttc tctactacct gccgctgacg
     2821 atcgccgaga cgcagggcta cttcaaggac gagggactcg acctcacgat ctcggatttc
     2881 gccggcggtt cgcgcgcgct gcaggcgctc gtcggcggca gcgccgatgt cgtatccggg
     2941 gcgttcgagc acaccatcac gatgcaggtc aagggccagc gcctgcgcgc cttcgtcctc
     3001 gaaggcaggg cgccgcagat cgtcctgggc atcaatccga agacgatgcc cgactacaag
     3061 acggtggccg acctgaaggg caagaagctc ggcgtcaccg cgcccggctc gtcgacccag
     3121 gtgctcgcca acttcgttct cgccaaagcc ggcctcaagc cgagcgacgt ctcgatcgtc
     3181 ggcgtcggtg ccggcaatgg cgccgtggcg gcaattcgct cgggccagat cgacgcgatc
     3241 tccaacctcg atccggtgat cacgctgctg gtccgctcgg gcgacctgaa gatcatctcc
     3301 gacacccgcg tcgtctcggt ggccgatcgg gtcttcggcg gcccgatgcc ggcggcgtgc
     3361 ctgtacacgc accagacttt catcgacaag tacccgaaga cggtgcaggc cctcaccaac
     3421 gcgatcgtcc gcgccgaccg ctggatccag aaggcgggtc cgagcgacat cgtcaaggcc
     3481 gtgcccgaga actacctgct cggcgatcgc gccgtctatg tcgacgcgtt cctcgccgcc
     3541 aagggcgcgc tttcgcccga cgggatgatc ccggaggccg gggccgagac ggcgcggcgc
     3601 gcgctcgcca gcatcgatcc cgagatcgct gcggcgaagg tcgacctgtc ggcgatctgg
     3661 acgaacgagt tcgtgcggcg cgccgacgcg aagtacgcga aggcctgacg ccttcgaggc
     3721 gcgccgccgg cgggatcagc ggccgtcgtc gccggcatcg gcgacggcga cgcagcggcg
     3781 ctgcgcatcg agccaggcca cggtctcgtt catgtcgagg gcatagatgc cgcgcgtgat
     3841 gtagatgaag tcgcgcaggg aatcgacgcc ctgcgaggct ttcgcgacga tgcgcctttg
     3901 cagatgatcc atcgaggccg gcgcggtgca gggcgtggcg accgacgcgg tatcgccggc
     3961 aatggaaacg cctgggaggc cggccgagag cgcgacgacg gccagggcga cggtggtggc
     4021 gaagtgtttc atgacggtgt cctcgggtgg ttgtgactgg gcgggtgggt ggttcgctgc
     4081 ggcgccgatt cggtgacgcc ggatgtcacc ttaggcaccg acgccgcggc cgtcatccac
     4141 catgcgacgg atgccgcttc gatcgccgcg aatccgcccc ggcgcgcgac gaagccggcg
     4201 gagtcgatcc gcgtcggcgc cctacactcg cggacgctgc acggcgcgat tcctttcccg
     4261 gccggcagcg caaccccgaa cccgcgatgt ccgccgtccc cgcgcttgcc ttcgacaagg
     4321 tggtctgcac tttcgtctcg aaagacgatc ccggccagcg ctacacggcg gtgagcgacg
     4381 tgaccctgtc cgtcgggccc ggcgaattcg tctcggtcgt cggtccgacg ggctgcggca
     4441 agagcacgct gctcaacgtc gccgccggat tgctggcgcc gagcggcggc agcgtcgagg
     4501 tcttcggcga gccgctgcgc ggcatcaacc ggcgcgccgg ctacatgttc cagtcggaaa
     4561 gcctgatgcc gtggcgaacc gccatcggca atgtggcggc cgggctcgaa ttccgcggcg
     4621 tcggcgatgc cgcggggcgc gccgccgaat ggctgcgccg ggtcggcctc ggcggcttcg
     4681 ccgatcgcta tccgcaccag ctctcgggcg gcatgcgcaa gcgcgtgagc cttgcgcaga
     4741 cgctggtgct cgatcccgac atcatcctga tggacgagcc gttctccgcg ctcgacgtcc
     4801 agacgcgcca gctgatggaa aacgagctgc tcgcgctgtg ggcgcaaaag cgcaaggcgg
     4861 tgctcttcat cacgcacgac ctcgacgagg cgatcgcgct gtcggaccgc gtggtggtgc
     4921 tgtcggccgg gccgg
//
LOCUS       DASRWS010000567         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4241631, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000567 DASRWS010000000
VERSION     DASRWS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4241631"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFF72_10800"
     CDS             complement(<1..857)
                     /locus_tag="VFF72_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZV93696.1"
                     /translation="MTLTDLDADALSRAIHARETSCRDVMQAYLARVAAVNPGVNAIV
                     SLQDENDLLRQADACDAELRRGESRGWMHGMPQAIKDAAETRGIPSTLGSPLLKDHVP
                     GRDALMVERMKASGCIVVGKTNTPEFGLGSHTFNEVFGTTRNAFDRSRSAGGSSGGAA
                     VALATHMLPVADGSDFMGSLRNPAGWNDVYGFRPSQGRVPRWPSADVWVALLSTEGPM
                     GRTVGDVAALLDIQAGWDARVPTAIATKESFAAGLGGFDPRGVRIGWLGDLDGYLAME
                     PGIAGLCERG"
     gene            complement(908..2146)
                     /locus_tag="VFF72_10805"
     CDS             complement(908..2146)
                     /locus_tag="VFF72_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZV93697.1"
                     /translation="MTESTSPKLAVVGAGPVGLALAIHAANALPEASITLFDARTGQH
                     DVAADPRTLALSLGSVQLLERLEAWRAGGAQPIVEVHVSQQAPSLAATLLARLGEPAL
                     TIRAVDEGVPMLGAVLSYGAIVAPLQEVWLDAVAREPKRLVSRFGSPVAALKNVAGGA
                     EVDAGIAERFDLAVVAEGGVFAAAARPALVAGSRWPALRHDYGQTAWVGTVRFEGGSA
                     APGDSTRGIAFERFTRHGPAALLPLADARAALVWCVASDDDPVRDLDAAQRTVVLNTV
                     FHPSTPRIAEVSPLKSFALGLIAEKTLTDGRIVRIGNAAQTLHPVAGQGLNLGMRDAF
                     ELVRSLSRRTDVDAVLRQLEWQRAPDRWAMIGATDFLARSFTWTLPGAAAARGLGLRM
                     IQALGPVKSSIARQMMFGSR"
     gene            2241..>2857
                     /locus_tag="VFF72_10810"
     CDS             2241..>2857
                     /locus_tag="VFF72_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZV93698.1"
                     /translation="MALMDFIKKQFIDIIEWTEDGDGVLAWRFPMEDREIQNGGSLTV
                     RESQMAVFVNEGKIADVFSPGRYTLTTATLPILTYLQNWDKLFKSPFKSDVYFFSTRQ
                     QIDQRWGTSQPVTIRDKDFGAVRLRAFGNYAYRIADPRLFYNEVSGTRERYTIADIDG
                     QLRGLMLQDISNAVGASGTPFLDLAANQTAFAAALQAAATPAFEKL"
BASE COUNT          460 a         1005 c          973 g          419 t
ORIGIN      
        1 ccccgctcgc acagcccggc gatgcccggc tccatcgcca ggtagccgtc gaggtcgccg
       61 agccagccga tgcgaacgcc acgtggatcg aagccgccca gcccggccgc gaacgactcc
      121 ttcgtggcga tcgccgtcgg cacgcgcgcg tcccatcccg cctgtatgtc gagcaacgcc
      181 gccacgtccc cgacggtcct ccccatcggg ccctcggtgc tcagcaacgc cacccagacg
      241 tcggccgagg gccagcgcgg cacgcgcccc tggctcggcc gaaagccgta gacgtcgttc
      301 cagcctgccg gattgcgcag gctgcccatg aagtcgctgc catcggccac gggcagcatg
      361 tgcgtggcca gcgccacggc ggcaccgccg ctgctgccgc cggccgaacg gctgcgatcg
      421 aaggcattgc gcgtcgtgcc gaagacctcg ttgaaggtgt gcgagccgag accgaactcg
      481 ggcgtgttgg tcttgccgac gacgatgcag ccggacgcct tcatccgctc gaccatcagc
      541 gcgtcacggc cgggaacatg gtccttcagc agcggcgagc cgagcgtgct cgggatgccc
      601 cgggtctccg ccgcgtcctt gatcgcctgt ggcatgccgt gcatccagcc gcgactctcg
      661 ccgcgccgga gctcggcgtc gcaggcatcg gcctggcgca gcagatcgtt ctcgtcctgc
      721 aggctgacga tcgcgttgac gccgggattg acggcggcga cgcgcgccag gtaggcctgc
      781 atcacgtcgc ggcaggaagt ctcgcgggcg tggatggcgc gcgagagcgc gtcggcgtcg
      841 aggtcggtga gggtcaagga attgtcctcc gcggtggatt ggcgcactcg tcgcgcggcc
      901 tgccggttca gcgtgagccg aacatcatct gccgggcgat tgacgatttc acgggtccga
      961 gcgcctggat catccgcaag ccgaggccgc gcgcggcggc ggcgccgggc aatgtccagg
     1021 tgaagctgcg ggcaaggaaa tcggtggcgc cgatcatcgc ccagcgatcg ggggcgcgtt
     1081 gccattcgag ctggcgcagc accgcgtcga cgtccgtccg gcgggacagc gagcgcacca
     1141 gctcgaaggc atcgcgcatg cccaggttga gcccctgtcc ggcgaccgga tgcaaggtct
     1201 gcgcggcgtt gccgatccgc acgatgcggc catccgtcag ggtcttctcg gcgatcagcc
     1261 cgagcgcgaa gctcttcagc ggcgagacct cggcgatgcg cggcgtcgaa ggatgaaaga
     1321 cggtgttgag gacgaccgtg cgctgtgccg cgtcgaggtc gcgcaccgga tcgtcgtcgc
     1381 tggccacgca ccagacgaga gcggcgcggg cgtcggccag cggcagcaac gcggcaggcc
     1441 cgtggcgcgt gaatcgttcg aaggcgatgc cgcgcgtcga atcgcccggc gccgccgagc
     1501 cgccctcgaa gcgcaccgtc ccgacccacg cggtctggcc gtagtcgtga cgcagcgccg
     1561 gccagcgact gccggccacg agagccggcc gcgccgctgc cgcgaagact ccgccttcgg
     1621 caacgaccgc gagatcgaaa cgctcggcga tgccggcatc gacttcggcg ccacctgcga
     1681 cgttcttcaa cgcagcgacc ggcgagccga aacgactgac gagccgcttc ggctcgcgcg
     1741 cgacagcgtc gagccagact tcctgcaacg gcgcgacgat cgcgccgtag ctcagcacgg
     1801 cgcccagcat cggcacgcct tcatcgacgg cccggatcgt cagtgcgggc tcgccgagac
     1861 gcgccagcag cgtggcggcg agcgaagggg cctgctgcga cacatgcact tcgacgatgg
     1921 gctgcgcgcc gccggctcgc cacgcctcga gccgctcgag caactgcacg ctgcccagcg
     1981 agagcgccag cgtgcgcgga tcggcagcga cgtcgtgctg cccggtccgc gcgtcgaaga
     2041 gcgtgatcga agcctcggga agcgcattcg cggcgtggat ggcgagcgcc agaccgaccg
     2101 gtccggcacc gaccacggcg agcttcggcg atgtcgattc cgtcatgcgc gctccccgtt
     2161 tttggggatt gtttatgcag tgtgcggcgt attattcgcc gcacccgaat gttcgggtca
     2221 cgagtgggag aaacgctgcc atggcgctga tggatttcat caagaaacag ttcatcgaca
     2281 tcatcgagtg gaccgaggac ggtgacggcg ttctcgcgtg gcgtttcccg atggaagacc
     2341 gggagatcca gaacggcggc tcgctcaccg tgcgcgaatc gcagatggcg gtcttcgtca
     2401 acgaaggcaa gatcgccgac gtcttctcgc cgggccgcta cacgctgacg acggcaacgc
     2461 tgccgatcct cacctacctg cagaactggg acaagctctt caagagcccg ttcaagagcg
     2521 acgtctactt cttctcgacg cgccagcaga tcgaccagcg ctggggcacc agccagccgg
     2581 tgaccatccg cgacaaggat ttcggcgccg tgcgcctgcg cgccttcggc aactacgcct
     2641 accggatcgc cgatccgcgc ctcttctaca acgaggtgtc gggaacccgc gagcgctaca
     2701 cgatcgccga catcgacggc cagttgcgcg gcctgatgct gcaggacatc tcgaacgcgg
     2761 tcggcgccag cggcacgccg tttctcgatc tcgccgccaa ccagacggcg ttcgccgcgg
     2821 cgctgcaggc cgccgccacg ccggctttcg agaagct
//
LOCUS       DASRWS010000568         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_808613, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000568 DASRWS010000000
VERSION     DASRWS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808613"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFF72_10815"
     CDS             complement(<1..793)
                     /locus_tag="VFF72_10815"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HZV93699.1"
                     /translation="MWHRLLALLGGLLGGLLGPGVARAVDLPADNAETLYHLYSGGGV
                     TAQGPAVLVRKSLFDKVSIAGSYYVDAVSNASIDVVTTASKYHEVRSEYGLSADYVYR
                     DAQITLSGLSSHEPDYTANTGSVDISQEVFGGMTTVALGFTRGDDTVLKHNSPEFHDK
                     AKHWQYRLGVTQILTPRWLLSINGEALSDDGYLGSPYRDARVFGAFVPEKNPRTRSAR
                     AVKFRLVGDLGWRNAMHAEYRYYRDTWAIKSHTAELGYSQYLGNSW"
     gene            complement(807..1055)
                     /locus_tag="VFF72_10820"
     CDS             complement(807..1055)
                     /locus_tag="VFF72_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HZV93700.1"
                     /translation="MRATSPLALLALGAALALGGCAVPSLPKIEPWVKPYERERLADP
                     IMSLSRDPLSDKHIEHTRDVREGSRGATGVQGGGCGCN"
     gene            complement(1052..1570)
                     /locus_tag="VFF72_10825"
     CDS             complement(1052..1570)
                     /locus_tag="VFF72_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZV93701.1"
                     /translation="MRTILSSFLGRGARVAAALALLCGAGAAVPAIAPLSAAPDFTLH
                     TMKGPNLRLQEQRGRVVMVNFWATWCGPCRQEMPQLNRLYEKYHAAGFVLLGVNVDDD
                     TAKAAEVAAKLGVTFPVLLDTDKAVSKLYDLSTMPSTVLIDRDGKVRYVHRGYLTGYE
                     DSYEKQIRELLK"
     gene            complement(1583..2230)
                     /locus_tag="VFF72_10830"
     CDS             complement(1583..2230)
                     /locus_tag="VFF72_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HZV93702.1"
                     /translation="MKNNTMRIALLAAALAALAGTARAADPAPPPVDQVVVPQVDRRG
                     IHLPRFPSSDFEIGTFVGTYSTQNFGSSAVGGIRLGYHITEDFFVEGVYAATKVSDAN
                     FRQVLPGGVFPQEKETLSYYNLSVGYNILPGEIFIGGAHSFASQFYIITGIGSTKLDV
                     QRRPTINFGGGLRVYLTDHAALQLDVRDHVFSLDLLGKRQSTQNPELTLGVTAFF"
     gene            complement(2259..>2694)
                     /locus_tag="VFF72_10835"
     CDS             complement(2259..>2694)
                     /locus_tag="VFF72_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93703.1"
                     /translation="DDFLSRRVQLGVAWGEFRSEPTLKLWTSVRMSETLSLEGTVGQV
                     QGVFSGTDFWHVNIMTEPWSDRRFSPFAGVGVGRFTNFPNASLVNATTTSANLANAMV
                     GVRYYLTDRVVLRADYSIYTVFVNDARTTEYRAWTGGLSFFF"
BASE COUNT          431 a          828 c          982 g          453 t
ORIGIN      
        1 gccacgagtt gccgaggtac tggctgtagc cgagctcggc ggtgtgcgac ttgatcgccc
       61 aggtgtcgcg gtagtagcgg tactcggcgt gcatggcatt gcgccagccg aggtcgccga
      121 cgaggcggaa cttcaccgcc cgcgccgagc gcgtgcgcgg attcttctcg gggacgaagg
      181 cgccgaagac gcgggcatcg cgatagggac tgccgagata gccgtcgtcg gagagggctt
      241 cgccgttgat gctgagcagc cagcgcggcg tcaggatctg ggtcacgccg agtcggtact
      301 gccagtgctt ggccttgtcg tggaactccg gcgagttgtg cttgaggacg gtgtcgtcgc
      361 cgcgggtgaa acccagcgcg acggtcgtca tgccgccgaa gacctcctgc gagatgtcga
      421 cgctgccggt gttggcggtg tagtcgggct cgtggctgct caggcccgag agcgtgatct
      481 gcgcatcgcg atagacgtag tcggcgctca gcccgtactc gctgcgcact tcgtgatact
      541 tgcttgcggt ggtgacgacg tcgatcgagg cattgctgac ggcgtcgacg tagtagctgc
      601 ccgcgatgga gaccttgtcg aacaggctct ttcgcaccag caccgccggc ccctgtgccg
      661 tcacgccgcc gccgctgtag aggtgataga gggtttcggc gttgtcggcc ggcaggtcga
      721 ccgcgcgggc cacgccgggg cccagcaggc cgccgagcag gccgcccagc agcgccagca
      781 ggcgatgcca catgccgatg gcggccctag ttgcagccac agccgcctcc ctggacgccg
      841 gtggcaccgc gcgagccttc gcggacgtcg cgggtgtgct cgatgtgctt gtcggaaagc
      901 gggtcgcgcg acaggctcat gatcggatcg gcgagccgct cgcgctcgta gggcttgacc
      961 cagggctcga tcttcggcag cgacggcacg gcgcagccgc ccaacgccag cgcggcgccg
     1021 agagcgagca gggcgagcgg gctcgtcgct ctcacttcag caactcccgg atctgctttt
     1081 catagctgtc ctcgtagccg gtgaggtagc ctcggtggac gtagcggacc ttgccgtcgc
     1141 ggtcgatgag gaccgtggaa ggcatggtgc tcaggtcgta gagcttgctg accgccttgt
     1201 cggtgtcgag cagcacggga aacgtcacgc cgagcttggc ggcgacttcg gccgccttcg
     1261 ccgtgtcgtc gtcgacgttg acgccgagca gcacgaatcc cgccgcgtgg tatttctcgt
     1321 agaggcggtt gagctgcggc atctcctggc ggcacggccc gcaccatgtc gcccagaagt
     1381 tgaccatgac gacccggccg cgctgctcct gcaggcgcag gttggggccc ttcatggtgt
     1441 gaagcgtgaa gtcgggcgcc gccgaaagcg gcgcgatcgc cggcacggcg gcgccggcgc
     1501 cgcagagcag ggcgagcgcg gcggcaaccc gcgcgccacg gccgaggaag gaggacagga
     1561 tcgttcgcat gtcggtcggc gctcagaaga aggcggtgac gcccagggtc agctcggggt
     1621 tctgcgtgct ctggcgcttg ccgagcagat cgagcgagaa aacgtggtcg cggacgtcga
     1681 gctgcagtgc cgcgtggtcg gtgaggtaga cgcgcaggcc gccgccgaag ttgatcgtcg
     1741 gccggcgttg cacgtcgagc ttggtgctgc cgatgccggt gatgatgtag aactgcgacg
     1801 cgaacgaatg cgcgccgccg atgaagatct cgccgggcag gatgttgtag ccgaccgaga
     1861 ggttgtagta gctgagggtt tccttttcct gcgggaagac gccgcccggc agcacctgcc
     1921 ggaagttcgc gtcgctgacc ttggtcgccg catagacgcc ttcgacgaag aagtcctcgg
     1981 tgatgtggta gcccaggcgg atgccgccga ccgcgctcga gccgaagttc tgcgtcgagt
     2041 aggtgccgac gaaggtgccg atctcgaagt cgctgctcgg aaagcgcggc aggtggatgc
     2101 cgcggcgatc gacctgcgga acgacgacct gatcgaccgg cggcggcgcg ggatcggcgg
     2161 cacgggccgt gccggcgagc gcggccaggg ccgcggcgag gagggcgatg cgcatcgtgt
     2221 tgttcttcat gggcttcgtg aactctgctt ttcggcgtct agaagaagaa cgaaagtccg
     2281 ccagtccagg cgcggtattc ggtcgtcctc gcatcgttca cgaaaaccgt gtaaatcgaa
     2341 tagtcggcgc gcaacacgac ccggtcggtc aggtaatagc gcacgccgac catcgcattg
     2401 gcgaggttgg cgctggtcgt cgtcgcattg acgaggctgg cgttcgggaa attcgtgaag
     2461 cggccgacgc cgactccggc gaacggcgag aagcgccgat cggaccacgg ctcggtcatg
     2521 atgttgacgt gccagaagtc ggtgcccgag aagacaccct ggacctggcc gacggtgcct
     2581 tcgaggctga gcgtctccga catgcgaacg ctggtccaca gcttcagcgt cggctcgctg
     2641 cggaactcgc cccaggccac gccgagctgc acgcggcggc tgaggaaatc gtcg
//
LOCUS       DASRWS010000569         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2814238, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000569 DASRWS010000000
VERSION     DASRWS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2814238"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="VFF72_10840"
     CDS             complement(<1..2074)
                     /locus_tag="VFF72_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93704.1"
                     /translation="MPSLLLRLSWPELRHHRWRTAAALVAVALGVALAFSVHLINASA
                     LAEFDAATRAVGGEADLRIVGGTAGFDEALFGRVARSPAVALASPIVDVATSAVGADG
                     KRFALRVIGVDALTVAATAPALLPHVRHGAGRFAMLDPDAAFLNAEALRRIGAHRQRI
                     SITGAHGPVDLHVAGDVAASGGALAVMDIAGAQVALGMLGRLSAIDVRLVGGADRAAV
                     LRKLDLPADARVAAAGEAEQRASNVSRAYRVNLTVLALVALFTGAFLVFSILALSVAR
                     RQQQLALLGVVGLPARGRLALVLRESALLGVVGSALGLGLGTALALFALRVLGGDLGG
                     GYFAGVAPPLRFDTAGAVLYGSLGVVAAMVGGWLPARAAERLAPAQALKGLVANALPA
                     QRSLRRGLGLVLAGVVLAMLPPIVGLPLAAYVSIACLLIGGIACIPGLLGAILALVPA
                     PRRPLPLLAIERARHERDGATIAVAGVVASLALAVALTVMVTSFREAVSRWLDVVLPA
                     DLYARGAPGTIGGELSRLPPRLPDLAAALPGVRRVEAQRITSVLFSPTQPAVTIIARR
                     LADPSRDLPLAGALADAPPGSVPVYVSEAIVALYGWRRGSSFELPLPDGRSARIYVRG
                     VWRDYARQFGAVAIDRADWQRLTGDDSSNDLAIWLDATASTGAVEAGLRRLADAADGS
                     GERLEFASP"
     gene            complement(2189..2839)
                     /locus_tag="VFF72_10845"
     CDS             complement(2189..2839)
                     /locus_tag="VFF72_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93705.1"
                     /translation="MALLEVDAIARRYGDAPVFADVTFEVEAGEFVAVLGESGVGKST
                     LLNGIAGLDPIDAGRVRVLGTDVQGLAEPAQARFRRDHLGFVFQAFHVLPHLTVADNV
                     ALPLLLQGRDDAARVASMLVSVGLDGFGPRLPQTLSGGQLQRVAIARALVHRPELILA
                     DEPTGNLDPATADRVMTLFAAQVRAERAACVLVTHSRAAAARADRILTLTPEGIAP"
     gene            complement(2842..3801)
                     /gene="miaA"
                     /locus_tag="VFF72_10850"
     CDS             complement(2842..3801)
                     /gene="miaA"
                     /locus_tag="VFF72_10850"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZV93706.1"
                     /translation="MTRIRAPLGGARRVLCIAGPTASGKSAAALAVADALAPRRRVEI
                     VSVDSAQVYRGMDIGTAKPSEEERLRVPHHLMDIVDPAERYSAARFVDDATRVVDEIA
                     GRGALALLVGGTLLYFKALFEGLDEMPAADESVRGEIDRDARQRGWAALHADLAAIDP
                     ATAARLSPGDAQRIQRALEVHRVSGRPLSSWHGERKPSTPPPPLIALEPRDRGWLHAR
                     IADRFRAMLDAGFVDEVRRLRARGDLDASTPSMRAVGYRQAWAALDAGDLSTLAERGS
                     AATRQLAKRQLTWLRSMPWRQVVACDGDGATAEVVRLALRLAA"
     gene            complement(3798..5048)
                     /locus_tag="VFF72_10855"
     CDS             complement(3798..5048)
                     /locus_tag="VFF72_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZV93707.1"
                     /translation="MARIDPQPRPMPTRVFAALLTLLLGIQPVSTDLYLPALPTLRRD
                     LGASIEGAQLTLSVLIVCFGLGQLLCGPLSDRFGRRPVLLAGLAAYTIASFGAAASTG
                     IATLIVWRAVQGAAMAASVTCGRSIVRDLYDPAAGARMMSSALTGLGCIAVLSPLIGG
                     ALVEAFGWHAAMLAPAIFGAFTLAMIAWRLEETVPTLDATATRPANIVRNWRRVCANP
                     TFRAWALLSACTYGGLFCMLAGSSFVFIGMLGVERATFGIILASMSLAYIAGTFLCRR
                     LLLRHGLRGAVARGACLSLAGGGSMALLSLAGVHEVWAILLPQYVYALGHGVHQPCGQ
                     AGAVGPFPEKAGVAASLSGFAMTVTALGVGLWLGHALDVTVYPLTLGFGAFSVAVTVV
                     AWTLVRRHGEPAIAARARVAVRPA"
BASE COUNT          822 a         1787 c         1966 g          565 t
ORIGIN      
        1 gcggcgaggc gaattcgagg cgttcgccgg aaccgtccgc ggcgtcggcg aggcgacgca
       61 ggccggcctc gacggcgccg gtcgaggccg tggcgtcgag ccagatcgcc aggtcgttgc
      121 tgctgtcgtc gccggtcagg cgttgccagt cggcacggtc gatggcgacg gcgccgaact
      181 ggcgtgcgta gtcgcgccag acgccgcgga cgtagatccg ggccgatcgg ccgtcgggaa
      241 gcggcagctc gaaggaactg ccacgccgcc aaccatagag cgcgacgatc gcttcgctca
      301 cgtagacggg aacgctgcct ggcggtgcgt cggccagcgc gcccgcgagc ggcaggtcgc
      361 gtgacggatc ggccagccga cgggcgatga tcgtgacggc cggctgcgtc ggcgagaaga
      421 gcaccgacgt gatgcgctgc gcttcgacac ggcgaacacc cggcaatgcc gccgcgaggt
      481 cgggcaggcg tggcggcagc ctcgacaact cgccgccgat cgttccgggc gcgccgcgag
      541 cgtagaggtc ggccggcaaa acgacatcga gccatcggct gacggcctcg cgaaagctcg
      601 tcaccatcac cgtgagcgcg accgccaggg ccaggctggc gacgacaccg gcgacggcga
      661 tggtggcacc gtcgcgctcg tgccgcgccc gctcgatcgc cagcaggggc agcggtcgac
      721 gcggcgccgg cacgagggcg aggattgcgc cgagcaggcc cgggatgcag gcgataccgc
      781 cgatcagcag gcaggcgatc gagacatagg cagcgagcgg caggccgacg atcggcggca
      841 gcatcgccag gacgacgccg gcgaggacga gcccgagccc gcgacgcagc gaccgttgcg
      901 ccggcagggc gttcgcgaca aggcctttca gtgcctgtgc cggcgcaagc cgctccgccg
      961 cgcgggccgg cagccagcca ccgaccatcg ccgcgacgac gccgagcgaa ccgtaaagca
     1021 cggcgccggc cgtatcgaat cgcagcgggg gtgcgacgcc ggcgaaatag ccgccaccga
     1081 ggtcgccgcc gagaacgcgc agggcaaaca acgcgagggc ggtgcccagg ccgaggccca
     1141 gggcgctgcc tacgacgccg agcagcgccg attcgcgcaa cacgagggcc agacggcctc
     1201 gcgccggcag gccgacgaca ccgagcaggg cgagttgctg ctggcggcgg gcgaccgaca
     1261 gcgccaggat cgagaagacg agaaaggcgc cggtgaagag tgcgaccagc gccaggacgg
     1321 tcaggttgac gcgatatgcg cgcgagacgt tcgaggcgcg ctgctccgct tcgccagcag
     1381 cggcgacacg tgcatcggct ggcaagtcaa gcttgcgcaa caccgccgcc ctgtcggcgc
     1441 cgccgacgag acggacatcg atcgcgctca gtcggccgag catcccgagc gcgacctgcg
     1501 ccccggcgat gtccatgacg gcgagcgcgc cgccgctcgc cgccacgtcg ccggccacgt
     1561 ggagatcgac gggcccgtgc gcgccggtga tcgagatccg ttgacgatgg gcaccgattc
     1621 gtcgcagcgc ttccgcgttg aggaatgccg cgtccggatc gagcatcgcg aagcgccccg
     1681 cgccgtgccg aacgtgcggc agcaatgccg gcgcggtggc ggcgaccgtc agggcgtcga
     1741 cgccgatcac gcgcaacgcg aatcgcttgc cgtcggcgcc gacggcgctc gtcgcgacat
     1801 cgacgatcgg gctcgccagc gcgaccgccg gcgagcgggc gactcgcccg aagagcgctt
     1861 cgtcgaagcc ggcggtgcca ccgacgatgc gcaggtcggc ctcgccgccg acggcgcgcg
     1921 tcgccgcgtc gaattcggca agggcggacg cgttgatcag atggaccgaa aacgccagcg
     1981 cgacgccgag tgcgaccgcg acgagggctg ccgcggttcg ccagcggtgg tgacgaagct
     2041 cgggccacga caggcgcagc aggaggctcg gcatgtcagg gatgacgtcg ggggatcgac
     2101 tcgtctcccg tcgtcgatcc cgctctgcgg gtcgcccgac ggcgagctcg ggcggagacg
     2161 gccgacgaca gcgaacgacc gccccgcttc acggcgcgat gccctccggc gtcagcgtga
     2221 ggatgcgatc ggcgcgtgcc gcggcggcgc gtgaatgggt gacgaggacg caggcggcgc
     2281 gctcggctcg cacttgcgcc gcgaacaggg tcatgacgcg gtcggcagtg gccggatcga
     2341 gattgccggt cggctcgtcg gcgaggatga gctctggccg atggaccagc gcacgagcga
     2401 tggcgacgcg ctgcaactgg ccgccggaaa gcgtctgcgg caggcgcggc ccgaagccgt
     2461 cgaggccgac cgagaccagc atcgacgcga cccgcgcggc gtcgtcgcgg ccctgcagca
     2521 gcagcggcag cgcgacgttg tcggcgacgg tgagatgcgg caggacgtgg aaggcctgga
     2581 agacgaagcc gagatggtcg cgccggaagc gggcttgcgc cggctcggcg agcccctgca
     2641 cgtcggtgcc gaggacacgg acgcggccgg cgtcgatggg atcgagaccg gcgatgccgt
     2701 tgagcagcgt cgacttgccg acacccgact cgccgagcac ggcgacgaat tcaccggcct
     2761 cgacttcgaa cgtgacatcg gcgaagaccg gcgcgtcgcc gtagcgccgg gcgatcgcgt
     2821 cgacctcgag cagcgccatc gtcaggcggc gagccgcagc gcgagacgga cgacctcggc
     2881 cgtcgcgccg tcgccgtcgc aggcgacgac ctgccgccac ggcatcgagc gcagccacgt
     2941 cagctgccgc ttggccagct ggcgcgtcgc ggcgctgccg cgctcggcca gcgtcgacag
     3001 gtcgccggcg tcgagcgcgg cccacgcctg gcgatagccg accgcgcgca tcgacggcgt
     3061 cgaggcgtcg aggtcgccgc gcgcgcgcag gcggcgaacc tcgtcgacga atccggcgtc
     3121 gagcatggcg cgaaagcggt cggcgatgcg cgcgtgcagc cagccgcgat cgcgcggctc
     3181 gagcgcaatc agcggtggcg gcggcgtcga cggcttgcgc tcgccgtgcc acgacgacag
     3241 cggacggccg ctgacccggt gcacttcgag cgcgcgctgg atgcgctgcg cgtcgccggg
     3301 cgaaagccgc gccgcggtcg ccgggtcgat tgcggcgaga tcggcatgca gggcggccca
     3361 gcctcgctgc ctcgcgtcgc ggtcgatctc gccccgcacg ctttcgtcgg cggcgggcat
     3421 ctcgtccagg ccttcgaaca gggccttgaa atagagcagg gtgccgccca ccagcagcgc
     3481 cagggcgccg cgtccggcga tctcgtcgac gacgcgggtc gcatcgtcga cgaagcgcgc
     3541 ggccgaatag cgctcggcgg gatcgacgat gtccatgagg tgatgcggca cgcgcaggcg
     3601 ttcctcctcg ctcggcttcg cggtgccgat gtccatgccg cggtagacct gcgccgagtc
     3661 gacgctgacg atctcgacgc ggcgccgcgg tgccagcgcg tcggcgacgg ccagcgcggc
     3721 cgcgctcttg cccgaggccg tggggccggc gatgcagagc acgcgacgtg cgccgcccag
     3781 cggagcgcga atgcgcgtca cgccggccgc acggcgactc gcgcgcgcgc cgcgatggcc
     3841 ggctcgccgt gtcgccggac cagcgtccag gcgaccaccg tgacggcgac gctgaaggcg
     3901 ccgaagccca gcgtcagcgg atagacggtg acgtcgagtg cgtggccgag ccagaggccg
     3961 acgccgagcg ccgtcaccgt catcgcgaat cccgagagcg acgccgcgac gccggccttt
     4021 tcggggaacg gtccgaccgc gccggcctgg ccgcacggct ggtgcacgcc gtgtccgagc
     4081 gcatagacgt actgcggcag caggatcgcc cagacctcgt gcacgccggc caggctcagc
     4141 agcgccatgc tgccgccgcc ggccagcgac aggcaggcgc cgcgggcgac ggcgccgcga
     4201 agtccgtggc gcagcagcag cctgcggcag aggaaggtgc cggcgatgta ggccagcgac
     4261 atcgatgcca ggatgatgcc gaacgtggca cgctcgacgc cgagcatgcc gatgaagacg
     4321 aacgacgagc cggcgagcat gcagaagagg ccgccgtagg tgcaggccga gagcagggcc
     4381 caggcgcgaa aggtcggatt ggcgcagacc cgccgccagt tgcggacgat gttcgccggc
     4441 cgcgtcgcgg tggcgtcgag cgtcggtacg gtctcctcga gccgccacgc gatcatcgcc
     4501 agggtgaacg cgccgaagat cgccggagcg agcatcgccg cgtgccagcc gaatgcctcg
     4561 acgagggcgc cgccgatcag cggcgaaagc acggcgatgc agccgagccc cgtcagcgcg
     4621 ctcgacatca ttcgtgcgcc ggccgccggg tcgtagaggt cgcggacgat ggagcggccg
     4681 caggtgacgg aggcggccat cgccgcgcct tgcaccgccc gccagacgat cagcgtcgcg
     4741 attccggtcg acgcggcggc gccgaagctg gcgatcgtgt acgcggccaa gcccgccagc
     4801 agcaccggcc ggcgaccgaa gcggtcggaa agcggtccgc agagcaactg ccccaggccg
     4861 aagcagacga tgaggaccga cagcgtgagt tgcgcgccct cgatgctcgc cccgagatcg
     4921 cggcgcaggg tcggcagggc cggcaggtag aggtcggtgc tgaccggctg gatgccgagc
     4981 agcagcgtca gcagggcggc gaagacgcgg gtcggcatcg gccgcggctg gggatcgatg
     5041 cgggccatcc agcgagggta gcaggcagga cgcatgcggg ctcgccgcgt ggctgcagag
     5101 gcgggagggc gacggcagcg ttcgtctcgt cttccgcgcg
//
LOCUS       DASRWS010000570         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2814607, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000570 DASRWS010000000
VERSION     DASRWS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2814607"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="VFF72_10860"
     CDS             <1..1370
                     /locus_tag="VFF72_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZV93708.1"
                     /translation="NAIGAEPELTLSLLPSAHATVFILGADTGVTKSDLAIWRDHLGA
                     HAPTRFVVLNKIDALEDPLASVAQVEAQIEQQQRETARTLGVATQRVFPISARQALAA
                     RVSGDEMRLNESRLPILEAALGAQLLPQRRQVLEQVVQEGASQIETHVVRHIGDSRRQ
                     LAEQTLELRGLRGKNNAKVRLMIKRVDAETAEFEQSTTLLQAMRAVHARMLKDMLVEL
                     SSDRVRDEVAEMQASMGGSILNLGAKKAFLALCERLRGLLASSQRRNGEIREMLSASF
                     ARLNAEYGFSLALGKPLEFERFAGELQEIESNYVQYLGLTLALRLAQPKFMEQFRRML
                     ISKLRVVFENASSELELWNKAVSAQVDSQLRERRKAFKRRRETLEKIQTAAGELESRI
                     DEIEVQDQRFVQLQGRVTDLAEALREHAFATPLATDAAVPLDIPLFDDAVPAGDDGAA
                     MQRQA"
     gene            1396..>2439
                     /gene="mutY"
                     /locus_tag="VFF72_10865"
     CDS             1396..>2439
                     /gene="mutY"
                     /locus_tag="VFF72_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZV93709.1"
                     /translation="MSATLPLPAGFAERVVDWQATHGRHDLPWQRDRRPYPVWLSEVM
                     LQQTQVSTVLGYFDRFVSRFPDVRALAAASLDDVLALWSGLGYYGRARNLHRCAQVVV
                     AEHGGEFPRTSSGLAALPGIGRSTAAAIAAFCFGERVAILDGNVKRVLARVLAFDADL
                     AEAAAERRLWDAASALVPAEGVAAYTQGLMDLGATVCLPRAPMCLLCPVAGVCRAAHR
                     STQGRYPVKSRRSRRGRRENAWLELRWRGRVWLVQRPATGVWAGLWSLPEYESVDSLA
                     AATRDWPGRGEALPPFVHVLTHFDWHLHPWRWTFPSRTSVGTLAPWLAPWPAGRWFEI
                     ETARTLALPAPLRK"
BASE COUNT          340 a          853 c          874 g          372 t
ORIGIN      
        1 tgaacgcgat cggtgccgag cccgagctga cgctgagcct gttgccgtcg gcgcatgcga
       61 ccgtcttcat cctcggcgcc gatacggggg tcaccaagtc cgacctggcg atctggcgcg
      121 atcacctcgg cgcccacgcg ccgacgcgct tcgtcgtcct caacaagatc gacgcgctcg
      181 aggacccgct ggccagcgtc gcgcaggtcg aggcgcagat cgagcagcag cagcgcgaga
      241 cggcgcgaac cctcggcgtg gcgacccagc gcgtctttcc gatctcggcc cggcaggcgc
      301 tcgccgcgcg cgtctcgggc gacgagatgc gcctcaacga gagccggttg ccgatcctcg
      361 aggcggcgct cggtgcgcag ctgctgccgc agcgccggca ggtgctcgag caggtcgtgc
      421 aggaaggggc gtcgcagatc gagacgcatg tcgtgcgcca catcggcgat tcgcgccgcc
      481 agctcgccga acagacgctg gagttgcgcg gcttgcgcgg caagaacaac gccaaggtcc
      541 gcctgatgat caagcgggtc gatgccgaga ccgcggagtt cgagcagtcg acgacgctgc
      601 tccaggcgat gcgggcggtc catgcgcgca tgctcaagga catgctggtc gagctgtcgt
      661 ccgaccgggt gcgcgacgaa gtcgccgaga tgcaggcgtc gatgggcggc tcgatcctca
      721 acctgggcgc gaagaaggcg ttcctggccc tgtgcgagcg cctgcgcgga ttgctcgcgt
      781 cgtcgcagcg gcgcaacggc gaaatccgcg agatgctctc ggcatcgttc gcgcggctca
      841 acgccgaata cggtttcagc ctggcgctgg gcaagccgct cgaattcgag cgcttcgccg
      901 gcgagctcca ggaaatcgag agcaactacg tccagtacct cggcctgacg ctggcgctgc
      961 gcctggcgca gccgaagttc atggagcagt tccggcgcat gctgatcagc aagcttcgcg
     1021 tcgtcttcga gaatgcgtcg agcgagctcg agctgtggaa caaggccgtc tcggcgcagg
     1081 tcgattcgca gttgcgcgaa cggcgcaagg cgttcaagcg ccgccgcgag acgctcgaga
     1141 agatccagac ggctgccggc gagctcgagt cgcgcatcga cgagatcgag gtgcaggacc
     1201 agcgcttcgt ccaattgcag gggcgcgtca ccgacctggc cgaggccttg cgcgagcatg
     1261 cgttcgcgac gccgctggcc accgacgcgg ccgtgccgct cgacatcccg ctcttcgacg
     1321 atgccgtccc cgccggcgac gacggcgcgg cgatgcaacg ccaggcgtga gccggcgcgc
     1381 tgccggcgcg cgtcattgag cgcgacgctg ccgctgccgg cgggattcgc cgaacgcgtc
     1441 gtcgactggc aggcgacgca cggccgccac gacctgccgt ggcagcggga tcggcggccg
     1501 tacccggtct ggctctccga ggtcatgctg cagcagacgc aggtgtcgac ggtgcttggc
     1561 tacttcgatc gcttcgtctc gcgctttccc gatgtgcggg cgctcgcggc ggcgagcctc
     1621 gacgatgtcc tggcgctctg gagcggcctc ggctactacg ggcgggcccg caacctgcat
     1681 cggtgcgcgc aggtcgtcgt tgccgagcac ggcggcgagt tcccgcgcac gagcagcgga
     1741 cttgctgcct tgccgggcat cggccgttcg acggcggcgg cgatcgccgc cttctgcttc
     1801 ggcgagcggg tcgcgatcct cgacggcaac gtcaagcgcg tgctggcccg cgtgctggcg
     1861 ttcgacgccg acctcgccga ggccgcggcg gagcgtcgcc tgtgggacgc cgccagcgcg
     1921 ctcgttcccg ccgaaggcgt cgccgcgtac acgcagggcc tcatggatct cggcgccacg
     1981 gtgtgcctgc cgcgcgcgcc catgtgcctg ctttgcccgg tcgccggtgt gtgccgggcc
     2041 gcgcaccgtt cgacgcaagg ccgctatccg gtcaagagcc gccgctcccg tcgcggacgg
     2101 cgcgagaacg cctggctcga gctgcgctgg cgaggccgcg tctggctggt gcaacggccg
     2161 gcgaccggtg tctgggccgg cctctggagc ctgcctgaat acgagtcggt cgactcgctc
     2221 gccgccgcga cgcgcgattg gccggggcga ggcgaggcgc tgccgccgtt cgtccacgtg
     2281 ctcacccatt tcgactggca cctgcatccc tggcgctgga ccttcccgtc gcgaacgtcc
     2341 gtcgggacgc tcgcgccgtg gcttgcgcca tggccggccg ggcgctggtt cgaaatcgag
     2401 acggcgcgca cgctcgccct gccggcgccg ctcaggaag
//
LOCUS       DASRWS010000571         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_232729, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000571 DASRWS010000000
VERSION     DASRWS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232729"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..2317)
                     /locus_tag="VFF72_10870"
     CDS             complement(230..2317)
                     /locus_tag="VFF72_10870"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZV93710.1"
                     /translation="MASKRTAKASPSSGDAHAVTGGERHEVAGGRHPAMTTNQGLVIA
                     DNQNSLRAHPRGPTLLEDFILREKITHFDHERIPERVVHARASAAHGHFELTTSLARF
                     TTAKVLTEVGVKTPVFARFSTVAGGAGSVDTPRDVRGFAVKLYTREGNWDLVGNNIPV
                     FFIQDAMKFPDLVHAVKMEPDRGFPQAATAHDTFWDYMSLTPEAMHMVMWIMSDRTLP
                     RSLRMMEGFGIHSFRLLDADGVSTFVKFHWRPRLGLQSTIWDETVKIAGADPDFHRRD
                     LFEAIQSGRFPQWDLAVQLFTEEEARAFPFDHLDATKLIPEELVPLRTIGRMVLDRWP
                     DNFFAETEQVAFCPSHLVPGLDFSNDALLQGRLFSYLDTQLSRLGSANFHQIPVNAPK
                     CPFANQQRDGHMQVAQPSGRVAYEPSSLAADSPRESPGTGFRSAAVDEGGARGRVRAE
                     SFADHYSQGRLFFRSQTAPEQAHIASALVFELSKVEAAHVREAMVGHLRHIDESLAKR
                     VADGIGMERMPSAPAAAGPVRDMTASPALQIIGKMKPTLEGRAVGILVADGSDGKLVE
                     ALRKAAVDAGATVKVVAPRLGGVRLGDGSRRAADGQLAGTPSVIFDAVAVILSKEGAA
                     KLAREASAVDFVRDAFGHLKAIAVDAGGQALLQSAGVEADAGIVSAHDSGAFIAAAMT
                     RQWDREARVRTLA"
     gene            2567..2857
                     /locus_tag="VFF72_10875"
     CDS             2567..2857
                     /locus_tag="VFF72_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93711.1"
                     /translation="MSRHSTLLTGSFCLVAAALAGGCGGVILDSRVHPVDVRSMAPSP
                     GVDERGLVLHERSSLSRAERQARVMQARRSGNLEAPGDAAASIDAPSRPIRN"
     gene            complement(2894..3454)
                     /locus_tag="VFF72_10880"
     CDS             complement(2894..3454)
                     /locus_tag="VFF72_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZV93712.1"
                     /translation="MIVGISGASGFVYGVRLLQMLRRLDVETHLIVSVSAQTTMAYET
                     GWKIADVRALADHHHRHDDMAACVSSGSYRTLGMIVAPCSMKTLADIATATSSTLIAR
                     AADVMLKERRRLVLLARETPLHAAHLRNMLAVTEMGAIVAPPVPAFYSRPANLEEMID
                     HTLGRVLDLFDLDAGTVRRWSGSSRG"
     gene            complement(3642..>4269)
                     /locus_tag="VFF72_10885"
     CDS             complement(3642..>4269)
                     /locus_tag="VFF72_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HZV93713.1"
                     /translation="ARLQGRKRQRIARRIAELTGLPLALIEEKNLRIGDVAYFFEALR
                     PRGLIVGRLEARATGPMAASRSREWEFDPGIEAIAPPYTMAAMAYFGGELGLADEMRY
                     EVLSSDVHKQWNWNRGDDKGNSFATTSPDLARAMRRNPQLRVFVASGRYDLGTPYSAT
                     DWSLAQIDAPAEVLGRIRHRYYDAGHMMYTRQADLAQLDADVGAWLGG"
BASE COUNT          630 a         1428 c         1523 g          688 t
ORIGIN      
        1 tgtgcgcgaa gggcatgcgg ttcgacgggc ggaagcttcg gatactctaa gggctccccc
       61 gcgcgatcac agaggcgtcg caagatcgcc gaggcgcgtc gcacgcaacg gcgccgccgc
      121 tgcccgtggc agatgccgac aggagggctt cacgggaagt gcgccgtgcc cgcgatctcc
      181 ccggcattcg tcgcgagcag cgcgctcgcg acgaccggag tcttggcggt caggccagcg
      241 tgcgaacccg ggcctcgcgg tcccactgcc gggtcatggc ggcggcgatg aaggcgcccg
      301 aatcgtgcgc cgagacgatg ccggcatcgg cttcgacccc ggccgactgc agcagcgcct
      361 ggcctccggc atcgacggcg atcgccttca ggtggccgaa cgcgtcgcgc acgaagtcga
      421 ccgcgctggc ctcgcgcgcc agcttggcgg cgccttcttt cgataggatc acggcgacgg
      481 catcgaagat gaccgaaggc gttccggcaa gctgtccgtc ggcggcgcgc cgcgagccgt
      541 cgccgaggcg cacgccgccg agccgcggcg cgacgacctt gaccgtcgca ccggcgtcga
      601 ccgccgcctt cctgagcgcc tcgacgagct tgccgtcgct gccgtcggcg acgaggatgc
      661 cgacggcgcg tccctcgagc gtcggcttca tcttgccgat gatctgcagc gccggcgaag
      721 ccgtcatgtc gcgcaccggg ccggccgcgg ccggcgccga tggcatccgc tccatgccga
      781 tgccgtcggc aacgcgcttc gccagcgact cgtcgatatg gcgaaggtgg ccgaccatcg
      841 cctcgcgaac gtgcgccgct tccaccttcg agagctcgaa gacgagggcc gatgcgatgt
      901 gcgcctgctc gggcgccgtc tggctgcgaa agaagaggcg cccctggctg tagtggtcgg
      961 cgaacgactc ggcgcggacg cgaccgcggg cgccgccttc gtcgacggcg gcgctgcgaa
     1021 agcccgtgcc gggcgattcg cgcggcgaat cggcagccag cgagctcggc tcgtaggcga
     1081 cgcgtccgct cggctgcgcc acctgcatgt ggccgtcacg ctgctggttg gcaaagggac
     1141 atttcggtgc gttgacgggg atctggtgga aattcgccga gccgagcctc gacagctgcg
     1201 tgtcgaggta ggaaaaaaga cgcccctgca acagcgcgtc gttcgagaaa tccagtccgg
     1261 gcacgaggtg cgatgggcag aaggccacct gctcggtttc ggcgaagaaa ttgtccggcc
     1321 agcgatcgag caccatcctg ccgatcgttc taagcggcac cagctcttcg gggatgagct
     1381 tggtcgcgtc gagatgatcg aacggaaacg cccgggcctc ttcctcggtg aagagctgaa
     1441 ccgcgagatc ccactgcgga aagcgccccg actggatggc ctcgaacaga tcgcgccggt
     1501 ggaaatccgg gtcggcaccg gcgatcttca cggtctcgtc ccagatcgtc gattgcaggc
     1561 cgagcctggg tcgccagtgg aacttgacga aggtcgacac gccgtcggcg tcgagaaggc
     1621 gaaacgagtg gatgccgaag ccttccatca tgcgcagcga acgcggcagc gtgcggtcgg
     1681 acatgatcca catcaccatg tgcatcgcct ccggcgtcag cgacatgtag tcccagaagg
     1741 tgtcgtgcgc ggtcgctgcc tgcggaaagc cccggtcggg ctccatcttc acggcgtgaa
     1801 cgaggtcggg aaacttcatc gcgtcctgga tgaagaagac ggggatgttg ttgccgacga
     1861 gatcccagtt gccctcgcgg gtgtagagct tcaccgcgaa gccgcgcacg tcgcgcggcg
     1921 tgtcgaccga gcccgcgccg ccggcgacgg tcgaaaaccg cgcgaagacc ggtgtcttga
     1981 cgccgacctc tgtcaggacc ttcgccgtcg tgaatcgggc aagcgacgtc gtcaattcga
     2041 aatggccgtg cgccgccgac gcgcgggcat gtaccacgcg ctcgggaatg cgctcgtggt
     2101 cgaagtgggt gatcttctcg cgaaggatga agtcttcgag aagggtcggc cctcgcggat
     2161 gggcgcgaag cgagttctgg ttgtcggcga tgaccagacc ttggttcgtg gtcattgccg
     2221 ggtgccgtcc cccggcgacc tcgtggcgtt cgccaccggt gacggcatgg gcgtcgccgc
     2281 ttgaagggga ggctttcgcc gtgcgtttgc tggccatggt gttcggtccc gtcgtgatgc
     2341 gtgtcggcag gcagggcaac gcgcgttccc gcgatggtcc cggatcggcc cgcggcgcct
     2401 atcgtcgcga tagggccact gcgcggattc gttcacgggc ggcgtcgcgc ccaggcgcgg
     2461 attcgttcac gccgcgcgat tccgacaggg cgccgcgggc ggtacgcttt gcagcctgcg
     2521 tttcgactgc atccgccgaa cgcctcgggc cggcggaata ctcgccatga gtcgacattc
     2581 gaccctcctg accggatcgt tttgcctcgt cgccgcggcg ctggccggcg gctgcggtgg
     2641 cgtgatcctc gactcgcgtg ttcatccggt cgacgtgcgc tcgatggcgc cgtcgccggg
     2701 cgtcgacgag cgcgggcttg tgttgcacga acggtcgagc ctgagccggg ccgagcgcca
     2761 ggcgcgcgtc atgcaggcac gacgatcggg aaacctcgag gcgccgggcg atgcggcggc
     2821 gtcgatcgac gccccgtcgc gtccgatcag gaactgagga tcgccgcgac agcggccgct
     2881 cgcgcaagcg gcatcagccg cggctgcttc ccgaccaccg gcgcaccgtg ccggcgtcga
     2941 ggtcgaacag gtcgaggacc cgcccgaggg tgtggtcgat catctcctcg aggttcgcgg
     3001 gcctcgagta gaaggccggc acgggcggcg cgacgatcgc gcccatctcg gtgacggcca
     3061 gcatgttgcg caggtgcgcg gcgtgcaacg gcgtctcgcg cgccagcagc accagccggc
     3121 gcctttcctt gagcatcacg tcggcggcgc gcgcgatcag ggtcgacgac gtggcggtgg
     3181 cgatgtcggc gagcgtcttc atcgagcagg gcgcgacgat catgccgagc gtgcggtagg
     3241 agccgctgga cacgcaggcc gccatgtcgt cgtggcgatg gtgatgatcg gcgagcgcgc
     3301 gcacgtcggc gatcttccag cccgtctcgt aggccatggt cgtctgcgcc gagaccgaga
     3361 cgatcagatg cgtctcgacg tcgagccggc gcagcatctg cagcaggcgc acgccataga
     3421 cgaagcccga tgcgccgctg atgccgacga tgatccgcgg ccgcgacggt gcgcttgcga
     3481 cgggctccgg gcctgtccgc gcgtcgtcgt tcgccttggc cggcgctttc gcgccgcgtc
     3541 gccctttcgc tgcctcgctc acctgcgtcg cgtcctttcc gttcgtcgat gccggcagtg
     3601 cggtctcggc ggggccgatc ataggcggcc ggcgacggcg ttcagccgcc gagccaggcc
     3661 ccgacatcgg cgtcgagctg cgccaggtcg gcctggcgcg tgtacatcat gtgcccggcg
     3721 tcgtagtagc gatggcggat gcggccgagc acctcggcag gggcgtcgat ctgggccagc
     3781 gaccagtcgg tcgcgctgta gggcgtgccc aggtcgtagc ggccgctggc gacgaacacc
     3841 cgcagttgcg gattgcgtcg catcgcccgg gccagatccg gactggtcgt ggcgaagctg
     3901 ttgcccttgt cgtcgccgcg attccagttc cactgcttgt ggacgtcgct ggacagcact
     3961 tcgtagcgca tctcgtcggc gagtccgagc tcgccgccga aataggccat cgccgccatc
     4021 gtgtagggag gcgcgatcgc ctcgatgccc ggatcgaatt cccactcgcg cgaacggctc
     4081 gcggccatcg gcccggtggc gcgcgcctcg agcctgccga cgatcagccc gcgcggtcgc
     4141 agcgcctcga agaagtaggc gacgtcgccg atacgcaggt tcttttcctc gatcagcgcc
     4201 agcggcaagc cggtgagctc ggcgatgcga cgggcgatgc gctggcgttt tcgcccctgg
     4261 agccgggcg
//
LOCUS       DASRWS010000572         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4242618, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000572 DASRWS010000000
VERSION     DASRWS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4242618"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFF72_10890"
     CDS             <1..615
                     /locus_tag="VFF72_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HZV93714.1"
                     /translation="YATVIAEHFRDRGKNVLLLMDSLTRYAMAQREIALAIGEPPATR
                     GYPPSCFAKLPQLVERSGNGVAGGGSITAFYTVLSEGDDPQDPIADAARGILDGHIVL
                     SRELAEAGHYPAIDIERSVSRVMTSVADGGHLDSARRFRQLHARFQKARDLIQLGAYS
                     AGSDAELDLAVRLHDRMNELLQQNMNDAARLDDSVVQLHALLAA"
     gene            641..1111
                     /locus_tag="VFF72_10895"
     CDS             641..1111
                     /locus_tag="VFF72_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HZV93715.1"
                     /translation="MNDDLQTLDALLAQAERNRDEALAEQLKLEAARRGAEAQAEQLV
                     VYRREYEQRWSAEFCREGKIELVRCYQGFMARLTQAVEQQQRSARQAAAQVERAAALV
                     RGHDVRAAALRKLRERRLEEGHRSDLRIEQKQADEHAARIAWLRLGANPAQGAV"
     gene            1132..2541
                     /locus_tag="VFF72_10900"
     CDS             1132..2541
                     /locus_tag="VFF72_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZV93716.1"
                     /translation="MIDRTTRALAVVAAPAPASAPAAPRPGAGAQAPAAHPFAELLQQ
                     NRDAARAADASVGPAPPGAQRPQDTGRIAATDAKPREANSPAAKESTAKSTSAPAAST
                     DAPGSPTQDASTASTSPDATRGRSPGTDRADKTRDDATTRGDAADEDASANAAGSDTG
                     ATRGKDDANRTHGLAPADPGLVASQLAGRPAAPLGDAPAPVLAAQGHDAAAFGSATPD
                     AAAGGGASGGAAAAPGTPSDAAASTRAGAAEGARHDAGGDAGSVAADAALASGAFATT
                     LAEATHSHPAAAHGGQGFDALGSGALQSASTSPAASSGAGADGVATASLPTPLQSPAF
                     AAALGVQVSVLARDGVQRAELHLNPADMGPVSIRITLDGTQARVDFGADTAATRQAIE
                     AGLPQLASAMRDAGFTLAGGGVSQHSGSGTGSSGDGGDASGDRRPAMRVSATGTARLG
                     GAAEQLTRRRGQAGLDLYA"
     gene            2762..3400
                     /locus_tag="VFF72_10905"
     CDS             2762..3400
                     /locus_tag="VFF72_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HZV93717.1"
                     /translation="MSAVIAADAAAGAAPAKGGKKKLLVMVAVAVVVLLVLGAGTVVI
                     LKKRAAAHAAAQADDDGTAVSSAKAEKSHESMGPPTYLPLDPFVVNLADKEGDRYAQI
                     GITLEIESSVMAEQMRGYMPSIRNAILMVITRKTSRELLEPLGKEQLADEIAREAVRP
                     MGIEIAAPQPVTSASAASASTGGEAVMASTKRHGPTVKNPIRHVNFSSFIIQ"
     gene            3456..>3855
                     /gene="fliM"
                     /locus_tag="VFF72_10910"
     CDS             3456..>3855
                     /gene="fliM"
                     /locus_tag="VFF72_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853747.1"
                     /note="with FliG and FliN makes up the switch complex
                     which is involved in switching the direction of the
                     flagella rotation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HZV93718.1"
                     /translation="MNEQILSQDEVDALLQGISGESDSLEAAAPETGGVRTFDLANQE
                     RMVRDRMPTLEIVGERFAKNVRGGMFGFIRRSPEVSIGTVRVQKYSAFLREIAVPTNF
                     NIVSVKPLRGLGLIVCEPQLIFAVIDALFGG"
BASE COUNT          598 a         1466 c         1325 g          466 t
ORIGIN      
        1 tacgcgaccg tcatcgccga acatttccgc gaccgcggca agaacgtgct gctgctgatg
       61 gattcgctga cccgctacgc gatggcgcag cgcgagatcg cgctggcgat cggagagccg
      121 ccggcgacgc gcggctatcc gccgagctgc ttcgccaagc tgccgcagct cgtcgagcgc
      181 agcggcaacg gcgtcgccgg cggcggctcg atcaccgcct tctacaccgt gctctccgaa
      241 ggcgacgatc cgcaggatcc gatcgccgac gccgcccgcg gcatcctcga cggccacatc
      301 gtcctgtcgc gcgagctcgc cgaagccggc cactatccgg cgatcgacat cgagcgctcg
      361 gtgtcgcgcg tgatgaccag cgtcgccgac ggcggccacc tcgactcggc gcggcgcttt
      421 cgccagctgc acgcgcgctt ccagaaggcg cgcgacctga tccagctcgg tgcctacagc
      481 gccggcagcg atgccgagct cgacctcgcc gtgcgcctgc acgaccgcat gaacgagctg
      541 ctgcagcaga acatgaacga cgccgcccgc ctcgacgaca gcgtcgtcca gctgcacgcg
      601 ctgctcgccg cctgaatccc gaccccccac gcaacgaccc atgaacgacg atctccagac
      661 gctcgacgcc ctcctcgccc aggccgagcg caaccgcgac gaggccctcg ccgaacagct
      721 caagctcgag gcggcgcggc gcggcgccga ggcgcaggcc gagcagctcg tcgtctatcg
      781 gcgcgaatac gaacagcgct ggagcgcgga gttctgccgc gaaggcaaga tcgagctggt
      841 ccgctgctac cagggcttca tggcgcggct gacgcaggcc gtcgagcagc agcagcgctc
      901 ggctcgccag gcggcggcgc aggtcgagcg ggccgcggcg ctggtgcgtg gccacgacgt
      961 ccgtgccgcg gcgctcagga aactgcgcga gcgccgcctc gaggaaggcc atcgcagcga
     1021 tctgcgcatc gagcagaagc aggccgatga acacgccgcg cggatcgcct ggctgcgcct
     1081 gggcgccaac cccgcacagg gcgcggtcta gggcacggca agacaggcga gatgatcgat
     1141 cgcacgaccc gcgcgcttgc cgtcgttgcg gcgccggcgc ccgcctcggc accggcagcg
     1201 ccacggccgg gcgccggagc gcaggcgccc gctgcgcatc cgttcgccga gctgctgcag
     1261 caaaaccgcg acgcggcgcg cgccgccgat gcgagcgtcg gcccagcgcc gcccggagcg
     1321 cagcgaccgc aggacaccgg tcgcatcgca gcgaccgacg ccaagccgcg cgaagcaaac
     1381 tcgcccgcag cgaaggagtc gacggcgaag tcgacttcgg ccccggcagc atcgaccgac
     1441 gcacccggct cgccgacgca ggacgcatcg accgcgtcga catcgcccga cgcgacccgc
     1501 ggacgctcgc caggcaccga ccgcgccgac aagacccgcg acgatgcgac gacgcgtggc
     1561 gacgccgccg acgaggatgc gtcggcgaac gccgccggca gcgacaccgg cgcgacccgc
     1621 ggcaaggacg acgccaaccg cacccatggc ctggcgcccg ccgaccccgg cctggtcgct
     1681 tcgcaactcg ccgggcgacc cgcggcccct ctcggagatg cccctgcccc ggtcctcgct
     1741 gcgcaaggac acgacgccgc agccttcggc tccgcgaccc cggatgccgc cgcgggcggc
     1801 ggcgcttccg gtggcgcagc ggcagctccc ggcacccctt ccgacgcagc ggccagcacg
     1861 cgcgccggtg ccgccgaggg ggctcgacac gacgcgggcg gcgatgctgg ctcggtcgcc
     1921 gccgacgccg cgctcgcctc cggtgccttc gccaccacgc tcgccgaagc gacgcattcg
     1981 catccggcgg cggcgcatgg cgggcaaggc ttcgatgcgc tcggcagcgg cgcgcttcag
     2041 tcggcatcga cgtcgccggc cgcatcctcc ggcgccggcg ccgacggcgt cgccaccgcc
     2101 agcctgccga cgccgctgca gtcgcccgcc ttcgccgcgg cgctcggcgt ccaggtcagc
     2161 gttctcgccc gggacggcgt ccagcgcgcc gagctgcatc tcaatcccgc agacatgggg
     2221 ccggtctcga tccggatcac cctcgacggg acgcaggccc gggtcgattt cggagccgac
     2281 accgcggcga cgcgccaggc gatcgaggcc ggcctgccgc aactcgccag cgcgatgcgc
     2341 gatgccggat tcacgctcgc cggtggcggc gtctcgcagc attccggatc gggcaccggc
     2401 tcgtccggcg acggcggcga tgcttcgggc gatcgccgcc cggcgatgcg tgtctcggcc
     2461 acgggcacgg cacggcttgg cggcgcggcc gagcagctga cgaggcgccg cggccaggcg
     2521 gggctcgacc tctacgcctg agagccggca cttgccaggg tgcaacgccg cgacccggcg
     2581 cccgcgcccc ggagcgtggt gcgcggctgc accgccgcgc gtcgcgtcgc cgccttctgc
     2641 gcggcaaaag cccgccttat cgccgcttca gcggcggcgt cgctttcgat aatttcctca
     2701 agtcatccgc cacgctgccg atcgcggccg ccggcaggaa acgccccgag gagaagcatc
     2761 gatgtccgct gtgattgccg ccgacgccgc cgccggcgcc gcccccgcca aaggcggcaa
     2821 gaagaagctg ctcgtcatgg tggccgtcgc ggtcgtcgtg ctgctcgtgc tcggcgccgg
     2881 cacggtggtg atcctgaaaa agcgggccgc cgcccacgcc gcggcgcagg ccgacgacga
     2941 cggcacggcc gtgtcgtcgg cgaaggccga gaagtcgcac gagtcgatgg gaccgccgac
     3001 ctacctgccg ctcgatccgt tcgtcgtcaa cctggccgac aaggagggcg accgctacgc
     3061 ccagatcggc atcacgctcg agatcgagag ctcggtgatg gccgagcaga tgcgcggcta
     3121 catgccttcg atccgcaacg ccatcctcat ggtgatcacg cgaaagacgt ccagggagct
     3181 gctcgagccg ctcggcaagg agcagctcgc cgacgagatc gcccgcgagg ccgtgcggcc
     3241 gatgggcatc gagatcgccg cgccgcagcc cgtgacgagc gcgtcggcgg cgtcggccag
     3301 caccggaggc gaagcggtga tggcctcgac gaagcgccac ggcccgacgg tgaagaaccc
     3361 gatccgtcac gtcaacttct cgagcttcat catccaatga cgcagtttcg gccacgccgc
     3421 gccccggcct gcctcaccgc tttgcgagag ccggcatgaa cgagcagatc ctcagccagg
     3481 acgaggtcga cgcgctgctg caaggcatct cgggcgaaag cgattcgctc gaggcggcgg
     3541 cgcccgagac gggcggcgtc cgcaccttcg acctggccaa ccaggagcgg atggttcgcg
     3601 accgcatgcc gacactcgag atcgtcggcg agcgcttcgc gaagaacgtg cgcggcggga
     3661 tgttcggctt catccgcagg agcccggaag tctcgatcgg cacggtgcgc gtgcagaagt
     3721 acagcgcctt cctgcgcgag atcgcggtgc cgacgaactt caacatcgtc agcgtcaagc
     3781 cgctgcgtgg actcggcctg atcgtctgcg agccgcagct catcttcgcc gtgatcgacg
     3841 ccctgttcgg cggcg
//
LOCUS       DASRWS010000573         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1935267, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000573 DASRWS010000000
VERSION     DASRWS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935267"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFF72_10915"
     CDS             complement(<1..1340)
                     /locus_tag="VFF72_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HZV93719.1"
                     /translation="MRFIGAALALAFLLVAADRLPRAEAAPTIVGPSSTHIDLETGKG
                     RLIRLDHPASSVFIANPEIADIQVKSPSLVYIIGKAAGETSLFAVGDKDQVLLNAQIV
                     VRHDLARLESALRELVPDGTVGVSTVNNSLVLSGIVFSAAKADDIRRIAAHFVPDKSQ
                     LINNVEVDAPNQVNLRVRIAEISRQTIKALGFNWENVFGPGNFLLGLATGNPVLQAGA
                     LLAPGKAAQVAIPQIQNPAGTLPFLTRTNGVDNLVAGYQGGRTDLNVLIDALDKQGLV
                     TVLAEPNLTALSGQTASFLAGGEFPVPVPGENNTTTIQFKKFGVSLAFQATIGARDLI
                     SLKVKPEVSELSSAGAITLNSISVPALTTRRAETTIELGSGQSFAIAGLLQNNVTHDL
                     QKFPWLGDVPVLGQLFRSDRFQRDESELVIIVTPYIVRPVSSKRLMAPTDGYQAP"
     gene            complement(1352..2224)
                     /gene="cpaB"
                     /locus_tag="VFF72_10920"
     CDS             complement(1352..2224)
                     /gene="cpaB"
                     /locus_tag="VFF72_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZV93720.1"
                     /translation="MLLRRLAFLLIAIAVSVGTVLLARSWMETQLASRKPPTAVAQEL
                     PSKMVLVAKTDLPTGQFVRAENLRWQAWPEKGVAGSYVVEGKGKLEDYVGAVVRSSMS
                     AGEPITDGRLVRPGDRGFMAAVLTPGYRAVTVPVTPSSGIAGFAFPGDHVDVILTLTI
                     QPLPGKDGARSGPDRRASETILSDIRVLALDQRADDQKHQVTVAKTATLEVTPKQAEI
                     IAVASEMGKLSLSLRSLAQDTLASAETRPYTWDSDAATLLRARGTGTLSGRKVSVVRG
                     SKSTEVEFATAAIR"
     gene            complement(2240..>2497)
                     /locus_tag="VFF72_10925"
     CDS             complement(2240..>2497)
                     /locus_tag="VFF72_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93721.1"
                     /translation="GGDVKLLAATALWVPPALLAPFTLVVSFTGALLGIAMLTPLRRR
                     MPPPPAGLLGDTAAGALRQPMPFAVAIAAGGLFALLSRLAR"
BASE COUNT          369 a          793 c          940 g          395 t
ORIGIN      
        1 ggggcctgat agccgtctgt aggcgccatc aggcgcttgc tcgagacggg ccggacgata
       61 tagggcgtga cgatgatcac gagttccgac tcgtcccgct ggaagcggtc ggagcggaag
      121 agctggccga gcaccggcac gtcgccgagc caggggaatt tctgcaaatc gtgcgtgacg
      181 ttgttctgca gcagcccggc gatcgcgaag ctctggccgc tgcccagctc gatcgtcgtc
      241 tccgcgcgcc gcgtcgtcag cgccgggacg ctgatgctgt tcagcgtgat ggcgccggcc
      301 gaggacagct cgctgacctc gggcttgacc ttgaggctga tgaggtcgcg cgcgccgatc
      361 gtcgcctgga aagcgaggct gacgccgaat ttcttgaatt ggatggtggt cgtgttgttc
      421 tcgccgggga ccgggacggg gaactcgccg ccggcgagga agctggcagt ctgcccggag
      481 agggcggtga ggttgggctc ggcgagcacc gtgacgagcc cctgcttgtc gagcgcgtcg
      541 atcagcacgt tgaggtcggt gcggccgccc tggtagccgg cgacgaggtt gtcgacgccg
      601 ttggtgcgcg taaggaaggg cagggtcccg gcggggttct ggatctgcgg tatggcgacc
      661 tgcgccgcct tgccgggcgc cagcagcgcc cccgcctgaa ggaccggatt gccggtcgcg
      721 aggccgagca ggaagttgcc ggggccgaag acgttctccc agttgaagcc gagcgccttg
      781 atggtctggc gcgagatctc ggcgatccgc acccgcagat tgacctggtt cggggcgtcg
      841 acctcgacgt tgttgatgag ctgcgacttg tcggggacga agtgcgccgc gatgcggcgg
      901 atatcatcgg ctttagcggc cgagaacacg atgccgctca gcaccagcga gttgttgacc
      961 gtgctgaccc cgacggtgcc gtcgggaacc agctcgcgca gggcgctctc cagccgcgcc
     1021 agatcgtggc ggacgacgat ctgggcgttg agcaggacct ggtccttgtc gccgacggcg
     1081 aagaggctcg tctcgcccgc ggccttgccg atgatgtaga cgaggctcgg cgatttcacc
     1141 tggatgtcgg cgatctcggg attggcgatg aagacgctcg aggccgggtg gtcgaggcgg
     1201 atgaggcggc ccttgccggt ctcgaggtcg atatgcgtgc tgctcggccc gacgatggtc
     1261 ggcgcggcct ccgcgcgcgg cagccggtcc gccgcgacga gcaggaaggc gagggcgagc
     1321 gccgcgccga tgaatcgcat cgtccttctc ctcaccggat cgctgccgtg gcgaactcga
     1381 cctcggtgct cttgctgccg cggacgacgc tcaccttacg gccggaaagg gtgccggtgc
     1441 cgcgcgcgcg gagcagggtg gcggcgtcgg aatcccaggt gtagggcctg gtctccgccg
     1501 aggcgagcgt gtcctgggcg aggctgcgca ggctgagtga gagcttgccc atctcgctgg
     1561 cgacggcgat gatctcggcc tgcttggggg tcacctccag ggtcgccgtc ttggcgaccg
     1621 tgacttggtg cttctgatca tcggcccgct ggtcgagggc gaggacgcgg atatccgaaa
     1681 ggatggtctc gctggcgcgc cggtcggggc cgctccgcgc gccatccttg ccgggcagcg
     1741 gctggatcgt cagtgtcagg atgacgtcga catggtcgcc cggaaaggcg aagcctgcga
     1801 tgcccgagct cggcgtcacc ggcacggtga cggcgcggta tccgggcgtc aggacggcgg
     1861 ccatgaaacc gcggtcgccg ggccgcacga ggcggccgtc ggtgatcggc tcgccggccg
     1921 acatgctgct tcgcacgacc gcgccgacat agtcttccag cttgcccttg ccttcgacga
     1981 cgtagctgcc ggcgaccccc ttctcgggcc aggcctgcca gcgcaggttc tcggcgcgca
     2041 cgaactggcc ggtcggcagg tcggtcttgg cgaccaggac catcttgctc ggcagctcct
     2101 gggccaccgc cgtcggcggc ttgcgcgacg cgagctgcgt ctccatccag ctccgggcga
     2161 ggagaacggt gccgaccgag acggcgatcg cgatcagcag aaaggcgagg cggcgcagca
     2221 gcatgagact tactccgtcc tagcgggcga ggcgcgagag gagggcgaag agtccgcccg
     2281 ccgcgatcgc gacggcaaaa ggcatcggct ggcgcagcgc gccggcggct gtatccccga
     2341 gcaaccccgc cggcggcggc ggcatgcggc ggcgcagcgg cgtcagcatg gcgatcccga
     2401 gcagcgcacc ggtgaacgag acgacgagcg tgaagggcgc gagcagcgcc gggggaaccc
     2461 acagtgcggt cgccgccaga agcttcacgt cgccgcc
//
LOCUS       DASRWS010000574         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_809844, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000574 DASRWS010000000
VERSION     DASRWS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809844"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFF72_10930"
     CDS             complement(<1..205)
                     /locus_tag="VFF72_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93722.1"
                     /translation="MKFRFPVIIIDEDYRSENASGLGIRALARAIESEGTEVLGVTSY
                     GDLSQFAQQQSRASAFILSIDDEE"
     gene            complement(386..1210)
                     /locus_tag="VFF72_10935"
     CDS             complement(386..1210)
                     /locus_tag="VFF72_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZV93723.1"
                     /translation="MTPGVHAGLPRRAAGPTHARMSLALIVILAALAWLALAGWSASP
                     WRRWLEHGAWSDVAWLAALCRAVPAGEKMLPPAAYAVAWMLMIAAMMLPTILPLLAIF
                     RRVVASQPRAGALMAAVVVGYATAWFGFGVVAYGLDMAVREAAAASGWLVAHGWVIGA
                     VVIAAAGAFQFSALKYRCLDRCRSPFGFVNARWRGRRPFAESYRLGVGHGLFCVGCCW
                     ALMLVTFVVGMGNVGWMLVLAAAMAAEKNLPWGARLRTPLGVGLLAWAAAIAVANG"
     gene            complement(1257..1859)
                     /locus_tag="VFF72_10940"
     CDS             complement(1257..1859)
                     /locus_tag="VFF72_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZV93724.1"
                     /translation="MGYQLEGRLLEVCNCRVLCPCWIGEDPDNGTCDTIVAWHFDQGK
                     INGVDVSDRTIALIAHVPGNILQGNWRAAVYLDDRVTPAQEDAILAVYTGKLGGPVGD
                     LVKLVGEVVSVEKVPISFGVRGGNGTLKIGDAGYAELVPYQGAMGGPTTLSDTVFSTV
                     PGAPVFVGKAPVYRSKNAKLGIDLDISGYNALQSVFRFDA"
     gene            complement(2019..>2752)
                     /locus_tag="VFF72_10945"
     CDS             complement(2019..>2752)
                     /locus_tag="VFF72_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV93725.1"
                     /translation="WRIPLAIASIGALAAALAIGLAAVGPNRAPSPRIDPRAVLSAWR
                     DVPLRLANLGYLGHMWELYAMWAWIGVFLDASFALTMPPASAGVAAKLAAFATVASGA
                     IGCVGAGLLADRLGRTTLTIIAMAVSGSCAASIGLLFGGDPTVLVIVCIVWGISIVAD
                     SAQFSASIAELADPSRVGTMLTVQTALGFTLTLATIHLMPHFVATMGWRYAFVPLAIG
                     PAVGVWAMARLRARPEAARLAGGNR"
BASE COUNT          475 a          986 c          924 g          367 t
ORIGIN      
        1 tctcctcgtc gtcgatcgag aggataaagg ccgaggcgcg cgactgctgc tgcgcgaact
       61 gcgacaggtc gccgtagctc gtcacgccga gcacttcggt cccctcgctt tcgatcgcgc
      121 gcgccagtgc gcggatgccg agcccgctcg cgttctccga acgataatcc tcgtcgatga
      181 tgatgacggg aaagcgaaat ttcatccgtg cactcctctt aacgggtcag agccgcaata
      241 cctgcctcga acggagggca agtgttgcgg ctctgaccaa tagactggca ggcgcgccaa
      301 agttccgccc gcggcgcgcg actgcgcggt tttgtccggg ccgcattgtg cgctgcttcg
      361 cccggaggcg cgaggttttt tcgcgtcacc cgttggcgac agcgatggcc gccgcccacg
      421 cgagcaagcc gacgccgagc ggcgtgcgca ggcgggcgcc ccatggcagg ttcttctcgg
      481 cggccatcgc ggccgcgagc acgagcatcc atccgacgtt gcccatgccg acgacgaacg
      541 tcaccagcat cagcgcccag cagcagccta cgcaaaacag gccgtggccg acgcccagcc
      601 ggtagctctc ggcgaacggg cgccggcccc gccagcgcgc attgacgaat ccgaacggcg
      661 agcggcagcg gtcgaggcag cggtacttga gcgcgctgaa ctggaatgcg ccggccgcag
      721 cgatcacgac cgccccgatc acccagccgt gcgcgacgag ccagcccgac gccgccgccg
      781 cttcgcgcac cgccatgtcg aggccgtagg ccacgacgcc gaacccgaac caggccgtcg
      841 catagccgac gacgacggcc gccatcagcg ctcccgcccg cggttggctg gcgacgacgc
      901 ggcggaatat cgcgagcagc ggcaggatcg tcggcagcat catcgcggcg atcatcagca
      961 tccacgcgac cgcgtaggcg gcaggcggca gcatcttctc gcctgccggc acggcgcgac
     1021 agagcgcagc cagccacgcg acatcgctcc acgcgccatg ctcgagccag cgccgccacg
     1081 ggctcgcgct ccagccggcc agcgcgagcc acgcgagcgc cgcgaggatg acgatcagcg
     1141 cgagcgacat ccgcgcatgc gtcggccccg ccgcccggcg cggcaagcct gcgtggacgc
     1201 cgggcgtcat gtgcgtcgcg atcgccccga cggcgccggg gcgatgcccg gtgtcgtcag
     1261 gcgtcgaacc ggaacacgct ttgcagcgcg ttgtagcccg agatgtcgag atcgatgccg
     1321 agcttcgcgt tcttcgagcg atagaccggc gctttgccga cgaataccgg cgcgccgggc
     1381 accgtcgaaa agaccgtgtc ggaaagcgtc gtcgggccgc ccatcgcgcc ctggtagggc
     1441 acgagctcgg catagcccgc atcgccgatc ttcagggtgc cgttgccgcc gcggacgccg
     1501 aaggaaatcg gcaccttctc gaccgacacg acttcgccga cgagcttgac gaggtcaccg
     1561 accggtccgc cgagcttccc ggtgtacacg gcaagaatcg cgtcttcctg cgccggcgtc
     1621 acacgatcgt cgagataaac ggccgcgcgc cagttgccct gcaggatgtt gcccggcacg
     1681 tgcgcgatca atgcgatggt ccgatcggac acatcgacgc cgttgatctt gccctggtcg
     1741 aaatgccagg cgacgatcgt gtcgcaggtt ccgttgtcgg ggtcctcgcc gatccagcag
     1801 ggacaaagaa cgcggcagtt gcacacttcc agcaaacgac cttcgagttg ataacccatg
     1861 gccgactccc ttcctgtacg gttgcgcatc ccgcgccgcg gtgggtaacg cggggaacgt
     1921 catgctacgc ccggaaccgc cgcgtcgcca tacgtaatat tcggcatcgc gagcgccggg
     1981 aaaccgcagc gtggcggcga cccggcagct cccggtcgtc accggtttcc gccggcgagg
     2041 cgggccgcct ccggacgcgc gcgcagccgc gccattgccc acacgccgac ggccgggccg
     2101 atcgcgagcg ggacgaacgc gtagcgccag cccatcgtcg ccacgaagtg cggcatcagg
     2161 tggatcgtcg cgagcgtcag cgtgaagccg agcgcggtct ggaccgtcag catcgtgccg
     2221 acgcgcgaag gatcggcaag ctcggcgatc gacgcggaaa attgcgccga atccgccacg
     2281 atgctgatgc cccagacgat gcagacgatg acgagcacgg tcggatcgcc gccgaacagc
     2341 aacccgatcg atgccgcgca gctcccgctg accgccatcg cgatgatcgt gagcgtcgtc
     2401 cgcccgaggc ggtcggcgag cagcccggca ccgacgcagc cgatggcacc tgacgcgacc
     2461 gtcgcaaagg cggccagctt ggcggcgacg cccgccgacg ccggcggcat cgtcagcgcg
     2521 aagctcgcgt cgaggaacac gccgatccac gcccacatcg cgtagagctc ccacatgtgc
     2581 ccgaggtagc ccaggtttgc gaggcgcaac gggacgtcgc gccatgcgct cagcacggcg
     2641 cgcgggtcga tgcgcggcga cggcgcgcga ttgggcccga cggcggcgag cccgattgcg
     2701 agcgcggccg cgagcgcgcc gatcgacgcg atggccagcg gaatgcgcca gg
//
LOCUS       DASRWS010000575         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_233717, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000575 DASRWS010000000
VERSION     DASRWS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233717"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4701
                     /locus_tag="VFF72_10950"
     CDS             <1..>4701
                     /locus_tag="VFF72_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZV93726.1"
                     /translation="EPWMEELYRRVSDRQTMGSVVQELRASLGEVEKSIDTFFRNPAD
                     HGVLVDVPTQLASMRGVLSVLGMDQAAQALLRMRDEVDGLVSTEIDPDRVGQAGVFQR
                     LAGNLSALGFMIDMLSVQPHLAKSLFVYDAEAGTLAPVMGRSAGMPEQPAPVEPRLIE
                     QAQLLAFSSVREDVPLQEVTRDLERLSHEAQAADQPTLAAAVLKAQESLLKAADDPVK
                     LTTARGELSEALVDFVATSTEPASLEPVVAEPAPAPMKPIALVGELEHDEEMRDVFLE
                     EAREVIDDAKTATAELQEAPGDLGLLTTVRRAFHTLKGSSRMVGLKAFGEAAWACEQL
                     FNTQLAEQRAVEAPLLEFTTWVLDHLAAWVDDIAGHRDEGRSEGEVKLAADRVARRLQ
                     SGEESSDIALPMGLPADLPSRADLELGQPAAPVEPVSPPAEVAFELDLSGLDSADASA
                     PQVDNLPALDVQASAMFDRFEDARDDDAMQTTLANEQFRPEPHAGASIDLDLGNPTEP
                     AALFEALAPSHDDHRGDGSGPSSLMLDLSSGEALPAATLGSSGSVPLDLEIDAPAPAQ
                     EYDGETPGATEEHVKVVGPLRIGIPLFNIYLNEADELSRRLMTEVAEWAMELHRPPGE
                     TPIALAHSLAGSSATVGFADLSHLARTLEHALARTQVIGHGTDEEARLFVNAAEEIRR
                     LLHQFAAGFLKEPSPELVTRLADHEVSSAMRLEAITAASELAGGADSESPALPGPAVE
                     PLLSLDDVEPVGAPSPSISLSDEASDAGPAATSRTPVSDEAKAPHPVADTGTRFGADG
                     AHDGANGDALSSTGSASDTAAIASHPSSVAEPSPATPIMPTPSAASAEDRSSDAHPAP
                     DAPTRISTSESGDALAATATDDDESPDDPFEAAVSSFGALGPLGALGRAELKPLAAAP
                     TEPARPAQAARASTFGDVDDDIDAVDAVDLELFPIFEEEAQELLPKLAGQLRDWSRQP
                     AVSTHASACMRTLHTLKGGARLAGAMRLGEMAHRLETRIERLTANEGKADASDVETLQ
                     GRADAMAHAFDALRNRDAQAYADAVAAAATPDPLPVRDAGSAARAAAVAAPPTSGPDG
                     AAPSGDERSAPPSSAPATPEPAAWVAAAPSVTPVPATGAEARKSGKGRKGATAAQDAM
                     PSAAAVAAGTATASSAPIDWSRFTKTTAAVPAKPQERAQAAAQSAVRVRAPLLDRLVN
                     QAGEVSITRSRIESGVSQIKGSLTDLTENLERLRGQLRDIELQGEVQMTSRLEAAKAA
                     SQAFDPLEFDRFTRFQELTRMMAESVNDVATVQRTLQRSLETAEDELVAQARLTRDLQ
                     DDLLRTRMVEFEGLSDRLYRVVRLAAKETGKQVRLDIVGGSIEVDRGVLDRMTGAFEH
                     LLRNCVSHGIEAPDKRIAAGKDATGTIVVSLSQEGNEVGVEFRDDGAGLDLARIRDKG
                     VAMRLLDPGHSHDDAELANLIFTAGFTTVDNVTELAGRGVGMDVVRSDVIAMGGRIET
                     ATAAGQGTSFKLILPLTTAVTQVVMLRCGDNRIAVPSTLVEIVRRATPKEVQAAYASG
                     VYEVGDATLPFFWLG"
BASE COUNT          733 a         1676 c         1650 g          642 t
ORIGIN      
        1 gagccgtgga tggaggagct gtaccggcgc gtctccgacc ggcagacgat gggcagcgtc
       61 gtccaggaat tgcgcgcctc gctcggcgag gtcgaaaaat cgatcgacac cttcttccgc
      121 aacccggccg accacggcgt cctcgtcgac gtgccgacgc agctcgcgtc gatgcgcggt
      181 gtcctgtccg tgctcggcat ggaccaggcc gcgcaagcgc tgctgcgcat gcgcgacgag
      241 gtcgacggcc tcgtctccac cgagatcgac ccggaccgtg tcggccaggc gggcgtcttc
      301 cagcgcctgg cgggcaacct gagcgcactc ggcttcatga tcgacatgct gagcgtgcag
      361 ccgcatctcg ccaagtcgct cttcgtctac gacgccgagg ccggcacgct ggcgccggtg
      421 atgggccgaa gcgccggcat gccggagcag ccggcgccgg tcgagccgcg cctgatcgag
      481 caggcgcaac ttctcgcgtt cagctcggtg cgcgaagacg tgccgctgca ggaagtgacg
      541 cgcgacctcg agcggctgtc gcacgaggcg caggcggccg accagccgac cctcgccgcg
      601 gcggtgctga aggcgcagga atcgctcctc aaggccgccg acgacccggt caagctgacg
      661 accgcccgcg gcgagctttc ggaagcgctg gtcgacttcg tcgccacgtc gaccgagccg
      721 gccagcctcg agccggtcgt ggccgagccg gcgccggcgc cgatgaagcc gatcgcgctc
      781 gtcggcgagc tcgagcacga cgaggagatg cgcgacgtct tcctcgagga agcgcgcgag
      841 gtgatcgacg acgcgaagac ggccaccgcc gagctgcagg aagcgcccgg cgatctcggc
      901 ctgctgacga ccgtgcggcg cgccttccat accctcaagg gaagctcgcg catggtgggc
      961 ctgaaggcct tcggcgaagc cgcctgggcg tgcgagcagc tcttcaacac gcagcttgcc
     1021 gagcagcgcg ccgtcgaggc gcccctcctc gaattcacga cctgggttct cgaccacctg
     1081 gcggcctggg tcgacgacat cgccggccac cgcgacgaag ggcgcagcga gggcgaggtc
     1141 aagctcgccg ccgaccgcgt cgcgcgtcgc ctgcagagcg gcgaggaaag ctccgacatc
     1201 gccttgccga tgggcttgcc ggccgatctg ccgagccgcg ccgatctcga gctcggccag
     1261 cccgcggccc cggtcgagcc ggtgtcgccg ccggccgagg tcgcattcga gctcgacctc
     1321 tccgggctcg acagcgccga cgcatcggct ccgcaagtcg acaacctgcc cgcgctcgac
     1381 gtccaagcgt cggcgatgtt cgatcgtttc gaagacgccc gcgacgacga tgcgatgcag
     1441 acgacgctgg ccaacgagca gttccggccc gagccgcacg ccggcgcgtc gatcgatctc
     1501 gacctcggaa atcccaccga gccggccgcg ctgttcgagg cgctggcgcc gagccacgac
     1561 gaccatcgcg gcgacgggag cgggccgagc agcctgatgc tcgatctgtc gagcggcgag
     1621 gcgcttcccg cggcgacgct cggcagcagc ggctcggtgc cgctcgatct cgagatcgac
     1681 gcgccggcgc cggcgcagga gtatgacggc gagactcccg gcgcgactga agagcatgtc
     1741 aaggtcgtcg ggccgctgcg catcggcatt ccgctcttca acatctacct gaacgaagcc
     1801 gacgagctgt cgcggcggct catgaccgaa gttgccgaat gggcgatgga gttgcatcgt
     1861 ccgcccggcg aaacgccgat cgcgctggcc cactcgctgg ccggaagctc ggcgaccgtc
     1921 ggcttcgccg acctctcgca cctggctcgg acactcgagc acgcgctggc ccgcacccag
     1981 gtcatcggcc atggcaccga cgaggaggcg cgtcttttcg tcaacgccgc cgaagagatc
     2041 cgtcgcttgc tgcaccagtt cgccgccggc ttcctgaagg agccttcgcc cgagctggtg
     2101 acacggctgg ccgatcacga ggtcagctcg gcgatgcgcc tcgaggcgat caccgcggct
     2161 tccgagctcg ccggtggcgc cgacagcgaa tcgccggcgc tgccaggacc cgccgtcgag
     2221 cctctgctga gtctcgacga cgtcgagccg gtcggtgcgc cgtcgccgtc gatttcgctg
     2281 agcgacgaag cgtcggacgc cgggcccgcg gcgacgagcc ggacgccggt ctccgacgag
     2341 gcgaaggccc cgcatccggt cgccgacacg gggacgcgct ttggagcgga cggcgctcat
     2401 gacggcgcga acggggacgc gttgtcgtcg accggatcgg catcggacac cgcggccatc
     2461 gcttcgcatc catcgtcggt ggccgaaccg tcgccggcga caccgatcat gccgacgccg
     2521 tcggccgcgt ccgcagaaga tcgatcgagc gatgcgcatc cggcccctga cgcgccgacc
     2581 cgcatctcga cgagcgagtc cggcgatgcg ctcgcggcca cggccacgga cgatgacgaa
     2641 tcccccgatg acccgttcga ggcggcggtg tcgtcgttcg gcgcgctcgg acccctcggc
     2701 gcgctggggc gtgccgagct gaaaccgctg gccgcggcgc cgaccgaacc ggcgcgaccg
     2761 gcgcaggcgg cccgggcgtc gaccttcggc gacgtcgacg acgacatcga tgcggtcgac
     2821 gcggtcgatc tcgagctgtt tccgatcttc gaggaagagg cgcaggagct tttgcccaag
     2881 cttgccgggc aactgcgcga ctggtcgcga cagccggccg tttcgacgca tgcctcggcc
     2941 tgcatgcgga ccctgcatac gctgaagggc ggtgcccgcc tcgccggggc gatgcgactg
     3001 ggcgagatgg cgcatcgact cgagacgcgg atcgaacgcc tgaccgccaa cgaaggcaag
     3061 gccgacgcga gcgacgtcga gacgctgcag ggccgggccg atgcgatggc gcatgccttc
     3121 gacgcgctgc gaaaccgcga cgctcaggcg tacgccgacg ccgttgccgc tgctgcgacg
     3181 cccgatccgc tgccggtgcg cgatgccggc agtgccgcca gggcggcagc ggtcgccgct
     3241 ccaccgactt ccggtccgga cggtgccgcg ccgtcgggcg acgagcgatc ggcccctccc
     3301 tccagcgcac ccgcgacgcc ggagccagcc gcctgggtgg ctgcggcgcc ttcggtcacg
     3361 ccggtaccgg cgacgggggc ggaagccagg aagtcgggca aggggcgcaa gggcgcgacg
     3421 gcggcccagg atgcgatgcc ttcagcggct gcggtggccg cgggcactgc aacggcgtcc
     3481 tcggcgccca tcgactggtc gcgattcacg aagaccacgg cggccgtgcc ggccaagccg
     3541 caggagcgtg cccaggccgc agcccaatcg gcggtgcgcg tgcgcgcgcc cttgctcgac
     3601 cggctcgtca accaggccgg cgaggtcagc atcacacggt cgcggatcga gtcgggcgtg
     3661 agccagatca agggatcgct caccgacctc accgaaaacc tcgagcggct gcgcggccag
     3721 cttcgcgaca tcgaactgca gggcgaggtc cagatgacct cgcggctcga ggccgcaaag
     3781 gcggcatcgc aggccttcga tcccctcgag ttcgaccgct tcacgcgctt ccaggaactg
     3841 acgcggatga tggccgaatc ggtcaacgac gtcgcgaccg tgcagcgcac cctgcaacgc
     3901 tctctggaga cggccgagga cgagctggtg gcgcaggccc ggctgacccg cgacctgcag
     3961 gacgacctgc tgcgcacccg gatggtcgaa ttcgaaggcc tgtccgatcg cctctaccgg
     4021 gtcgtccggc tcgcggccaa ggagaccggc aagcaggttc gcctcgatat cgtcggtggc
     4081 tcgatcgagg tcgaccgcgg cgtcctcgac cggatgaccg gcgcgttcga gcatctgctt
     4141 cgcaactgcg tcagccacgg catcgaggca cccgacaagc ggattgccgc cggcaaggat
     4201 gcgaccggca cgatcgtcgt ctcgctgtcg caggaaggca atgaggtggg cgtcgagttt
     4261 cgcgacgatg gcgccgggct cgacctggct cgcatccgcg acaagggcgt ggcgatgcgc
     4321 ctgctcgatc cgggccattc gcacgacgac gccgagctcg ccaacctcat cttcaccgcc
     4381 ggcttcacga ccgtcgacaa cgtcaccgag ctggccgggc gcggcgtcgg catggacgtg
     4441 gtccgctccg acgtcatcgc catgggcggc cgcatcgaga cggcaaccgc agcaggccag
     4501 ggcacgtcgt tcaagctgat cctgccgctg accacggcag tgacgcaggt ggtgatgctt
     4561 cgctgtggcg acaaccgcat cgccgtgccg tcgacgctgg tcgaaatcgt ccgccgggcc
     4621 acgccgaagg aggtccaggc ggcgtacgcg agcggcgtct acgaggtggg cgacgcgacg
     4681 ctgccgttct tctggctggg c
//
LOCUS       DASRWS010000576         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3677520, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000576 DASRWS010000000
VERSION     DASRWS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677520"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFF72_10955"
     CDS             <1..369
                     /locus_tag="VFF72_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93727.1"
                     /translation="DDADRLVQLAAVLAVGSAAVWPATPATDALRAALPPEVRQSIAS
                     AIDWRSDTVSFDAVLHHGDADSLQGVLRQVAGRRGPIVSVRGLRPGEVDVPLASLVVE
                     RALSTNTAAAGGNASLMTLD"
     gene            500..1846
                     /locus_tag="VFF72_10960"
     CDS             500..1846
                     /locus_tag="VFF72_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZV93728.1"
                     /translation="MDGPAEAVPIDAATEEVARRSLLIRVARTAQLWVTAAVAGALAA
                     GAALGFRWLTQQVEWAVTGHRGGLVAIARELPPWHRALVCAAGGLLAGIVLAAGRRWA
                     SRGPAGVVNLDYIDAARAGRVDLNDRTTLSRTASALVSVGTGASIGREGAMVQLGAWF
                     ASWLARLAPIPEERRPTIMVCGIAAGIGAAYHAPIAGLVFVLELALGFFARHRVAPTL
                     IAAATSSGIIYWLADPRPLYAVPDLTFVRASLFGAVFSGLIFGGIGWALLQLLDQARR
                     RFARIASLPLRLGAGGVLVGLLSAVVPEVWGNGYSAVAGVFADQFALQALALIFVTKV
                     VATALSSGSGAIGGIFTPSLFVGATAGSLLAQIAGLWLPATIVGDPRVLAIIGMASVL
                     TAVTHAPLMSIVMVLEMTQQFHLTAPIMLACGAAYAVSTRFGVRPLYGNPIEGHQQ"
     gene            complement(1884..3353)
                     /locus_tag="VFF72_10965"
     CDS             complement(1884..3353)
                     /locus_tag="VFF72_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93729.1"
                     /translation="MSAAPMLEVDRLTKRFGGLVAVKELSFSIRPGEILGLIGPNGSG
                     KSTAMKAIMGVESPSSGSIKLDGVELAGLPAYKIARLGVGLVFQHSRPLNRQTVLENI
                     KVALLPDSLTQVFAGTEVDRRARAIAERVGLAGVIDRKPPTLPFADLRRLELAKAIAR
                     HPKLVMVDEPFAGLTASEVAAFSELIREFRSEGRAVLLVDHNVKGVSALVDRVLAMYL
                     GEWIAEGAAVDVMQDETVRRVYLGGALGASARPEAGFADKVPALQVEGVGVFYGKALA
                     LQDVSLHVHEGEFVSVVGLNGAGKTTLFNAISGLVAHSGAIRWSGERLGDRSAARIAR
                     DGIVQCPETRQLFGDMSVRENLDLGGNSLDDAERDERLEWLFDLFPILKSRQAQTART
                     LSGGEQQMLAIARALMMKPKLLILDEPTLGLAPVILETLSKALERLRTTTSISVLLGE
                     QNVTFALPHADRVYVLDHARIVWEGLPGRFEEEAAATYL"
     gene            complement(3350..4279)
                     /locus_tag="VFF72_10970"
     CDS             complement(3350..4279)
                     /locus_tag="VFF72_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93730.1"
                     /translation="MRALFSSRLFWIGILSLVVGAVLPLLASGYVLGLLTVAYYTAVF
                     AMSWDLLFGFANEVNFGPTFLIGLGAYTAGILDARLSPAISIWLCVAAGAGAAVVGGV
                     ILALPALRLRGPYFGLTTLVAVLILQNFIIVFASWTGGEIGLTVPDVMSIDAHVNYWI
                     AFGFMALSGAILLGLSRSPIGLILQASGQDAVQAGALGFNVAKHKLAAFIVSAFFSGL
                     AGALMVFYMGAASVGTFVDIGVGVQVIIAAVLGGRRTIVGAAIGAVFLIGMGELLRPL
                     GELATLIVSAIALAVILFFPDGFLGILRGGLRS"
     gene            complement(4285..>5009)
                     /locus_tag="VFF72_10975"
     CDS             complement(4285..>5009)
                     /locus_tag="VFF72_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93731.1"
                     /translation="VAGYWHADAYLAALAGIVVGILLAFATYFVTVRPIQRSARIAEA
                     EKEIFILTATLLWGIIIQEIVAYLFTNNAKTVLPIVQGVVMLFGVRTPSNELFTAVVC
                     WLVIGLLWLLVNRTRRGKAVLAASMNPRGVTLLGIELSSIYLTVWAIYGLLAGIAGIL
                     LGMFLGVSSYSVGPLTASAFAIVVLGGLGSVSGSLIAAYVVGYLETLTAYLISPAYRT
                     IPALLLLVVVMYVRPQGLFGRR"
BASE COUNT          781 a         1719 c         1792 g          717 t
ORIGIN      
        1 gacgacgccg accgactcgt ccagctggcc gcggtgctcg cggtcggcag cgccgccgtc
       61 tggccggcga cgcctgcgac cgacgccttg cgcgcggcac tgccgcccga ggttcgccag
      121 tcgatcgcct cggcgatcga ctggcgcagc gacacggtgt cgttcgacgc ggtccttcat
      181 catggcgacg ccgactcgct tcaaggcgtc cttcggcaag tcgccggccg ccgtggcccg
      241 atcgtctccg tgcgcgggct gcgaccggga gaagtcgatg tgcctttggc gagcctcgtc
      301 gtcgagcgcg cgctcagcac caacaccgcc gccgccgggg gcaatgcgag cctgatgacg
      361 ctcgattgag acggcgctgt cggtcgcgcc tgccgctaaa gcaaaatcga agtcgccccc
      421 ggcgtcgacc cgccgcggtt cggcactgtt tccacagcga atgcgagaac accagcgagt
      481 gacccaagcc gagacagacg tggacgggcc ggccgaggcc gtaccgatcg acgcagccac
      541 cgaagaggtc gcccggcgct cgttgctcat tcgcgtggcc cggaccgcgc agctctgggt
      601 gacggccgcc gtcgccggcg ccctcgccgc cggggcggcg ctcggctttc gctggctcac
      661 gcaacaggtg gagtgggcgg tcacgggaca tcgcggcgga ctcgtcgcga tcgcccgcga
      721 gctgccgccc tggcatcggg cgctcgtctg cgccgccggc ggcctgctcg cggggatcgt
      781 gctggccgcg gggcgtcgct gggcgtcgcg agggccggca ggcgtggtca atctcgacta
      841 catcgacgcc gcgcgcgccg gccgggtcga cctgaacgac cggaccacgc tgtcgcggac
      901 ggcctcggcg ctggtctcgg tgggaacggg cgcgtcgatc ggccgcgagg gcgcgatggt
      961 gcagctcggc gcctggttcg cgtcgtggct ggctcgcctg gcgccgatcc cggaagagcg
     1021 gcggccgacg atcatggtct gcggcatcgc cgccggcatc ggcgcggcgt atcacgcgcc
     1081 gatcgccgga ctcgtcttcg tgctggagct cgcgctcggc tttttcgcgc gccatcgcgt
     1141 cgcgccgacg ctgatcgccg cggccacctc gagcggcatc atctactggc tcgccgatcc
     1201 gcggccgctc tatgccgttc ccgatctgac gttcgtgcgc gccagcctct tcggtgccgt
     1261 gttctcgggc ctcatcttcg gcggcatcgg ctgggcgctg ctgcaacttc tggatcaggc
     1321 acgcaggcga ttcgcccgca tcgcgtcgct gccgctgcgc ctcggcgccg gcggcgtcct
     1381 cgtcggcctt ctctcggccg tcgtccccga ggtctggggc aacggctaca gcgccgtcgc
     1441 aggcgtcttc gccgaccagt tcgccctgca ggcgctcgcc ctcatcttcg tgacgaaggt
     1501 cgtcgccacc gcgctcagtt cgggcagcgg cgcgatcggc ggcatcttca cgccgtcgct
     1561 cttcgtcggc gccaccgcgg gcagcctgct ggcgcagatc gccgggctgt ggctgccggc
     1621 gacgatcgtc ggcgatccac gcgtcctcgc catcatcggc atggcgtcgg tgctgaccgc
     1681 ggtgacgcac gcgccgctga tgtcgatcgt gatggtgctc gagatgacgc agcagttcca
     1741 cctgacggcg ccgatcatgc tggcctgtgg cgctgcctac gcggtcagca cgcggttcgg
     1801 ggtgcgtccg ctctacggca atccgatcga agggcaccag caatgagccg acttgcgccg
     1861 cggccgcgcg ccgcgatgtc ggctcagaga taggtcgccg cggcctcttc ctcgaagcgt
     1921 ccgggaagcc cttcccagac gatgcgcgcg tggtcgagca cgtagacgcg gtcggcgtgc
     1981 ggcagcgcga aggtgacgtt ctgctcgccg agcaggacgc tgatcgaggt cgtcgtgcgc
     2041 agccgctcga gcgccttgga gagcgtctcg aggatcaccg gcgcgagccc cagcgtcggt
     2101 tcgtcgagga tgaggagctt gggcttcatc atcagggcgc gggcgatggc cagcatctgc
     2161 tgctcgccgc cgctcagcgt tcgcgccgtc tgcgcctggc ggctcttcag gatcgggaac
     2221 aggtcgaaca gccactcgag tcgctcgtcg cgctcggcgt cgtcgaggct gttgccgccg
     2281 aggtcgaggt tctcgcgcac gctcatgtcg ccgaacagct ggcgcgtctc ggggcactgc
     2341 acgatgccgt cgcgggcgat ccgcgcggcg ctgcgatcgc ccagccgctc gccgctccag
     2401 cggatcgcgc ccgaatgggc gacgaggccg gagatcgcgt tgaagagcgt cgtcttgccg
     2461 gccccgttga gaccgacgac ggacacgaac tcgccttcgt ggacgtgcag cgagacgtct
     2521 tgcagggcca gcgccttgcc gtagaagacg ccgacgcctt cgacctgcag cgccggcacc
     2581 ttgtcggcga agcccgcttc ggggcgggcg ctggcgccga gcgcgccgcc gagatagacg
     2641 cggcgcaccg tctcgtcctg catgacgtcg acggccgcgc cttcggcgat ccattcgccg
     2701 aggtacatcg ccaggacccg gtcgacgagc gccgagacgc ccttgacatt gtggtcgacc
     2761 agcaggacgg cgcgaccttc gctgcggaac tcgcggatga gctcggagaa ggcagcgacc
     2821 tccgacgcgg tgaggccggc gaacggctca tcgaccatca ccagcttcgg atggcgcgcg
     2881 atcgccttgg ccagctcgag gcggcgcagg tcggcgaagg gcagcgtcgg cggcttgcgg
     2941 tcgatgacgc cggccagtcc gacgcgctcg gcgatcgcgc gcgcccgccg atcgacctcg
     3001 gtgccggcga agacctgggt caggctgtcg ggaagaagcg cgaccttgat gttctcgagc
     3061 acggtctgcc ggttcagcgg ccgcgaatgc tggaagacga ggccgacgcc gagacgggcg
     3121 atcttgtagg ccggcagccc cgcgagctcg acgccatcga gcttgatcga gccgctgctc
     3181 ggcgactcga cgcccatgat cgccttcatc gccgtcgact tgcccgaacc gttcggcccg
     3241 atcaggccga ggatttcgcc cgggcggatc gaaaagctca gctccttgac cgcgaccagc
     3301 ccgccgaagc gcttggtcag ccggtcgacc tcgagcatcg gcgccgcgct cacgaacgca
     3361 gcccgccgcg caggatcccc aggaagccgt cggggaagaa caggatcacc gccagcgcga
     3421 tcgccgagac gatgagcgtc gccagctcgc cgagcggacg aagcagctcg cccatgccga
     3481 tcaggaagac cgcgccgatc gccgcgccga cgatggtgcg ccggccgccg agaacggcgg
     3541 cgatgatgac ctgcacgccg acgccgatgt cgacgaaggt gccgaccgac gcggcaccca
     3601 tgtagaagac catgagcgcg ccggcgaggc cggagaagaa ggcgctgacg atgaaggcgg
     3661 cgagcttgtg cttggcgaca ttgaagccga gcgcgccggc ctgcaccgcg tcctggccgc
     3721 tcgcctgcag gatcaggccg atcggcgagc gcgacaggcc gagcaggatg gcgccgctga
     3781 gcgccatgaa gccgaaggcg atccagtagt tgacgtgcgc gtcgatcgac atcacgtcgg
     3841 gcaccgtgag gccgatctcg ccgccggtcc agctggcgaa gacgatgatg aagttctgca
     3901 ggatcagcac cgccaccagt gtcgtcaggc cgaaataggg accgcgcagg cgcagcgcgg
     3961 gcaacgccag gatgacaccg ccgacgaccg ccgcgccggc gccggcggcg acgcacagcc
     4021 agatcgagat cgcgggcgag agacgtgcgt cgaggatacc ggcggtgtag gcgcccaggc
     4081 cgatcaggaa ggtcggtccg aagttgacct cgttggcgaa gccgaagaga aggtcccacg
     4141 acatcgcgaa gactgccgtg tagtaggcga cggtgagcag gcccagcacg tagccggacg
     4201 cgagcagcgg caggacggcg ccgacgacga ggctgaggat gccgatccag aaaagccgcg
     4261 aggagaagag cgcgcgcacg gaggtcacct cctgccgaag agtccctgcg gtcgcacgta
     4321 catgacgacg acgagcagga gaagtgccgg gatggtccgg tacgccggcg agatcaggta
     4381 ggcggtcagc gtctcgaggt agccgaccac gtaggcggcg atcagcgagc ccgagacgct
     4441 gccgaggccg ccgaggacga cgatcgcgaa ggcgctcgcc gtcagcggcc cgacgctgta
     4501 cgagctgacg cccaggaaca tgccgaggag gatgccggcg attccggcga gcagcccgta
     4561 gatcgcccag accgtgaggt agatgctcga cagctcgatg ccgagcagcg tgacgccgcg
     4621 cgggttcatc gatgccgcga gcaccgcctt gccgcgccgg gtgcggttga cgagcagcca
     4681 gagcaggccg atgaccagcc agcagacgac ggcggtgaag agctcgttcg acggcgtgcg
     4741 cacgccgaac agcatcacca cgccctggac gatcggcagc accgtcttcg cgttgttggt
     4801 gaagaggtag gcgacgatct cctggatgat gattccccag agcagcgtcg ccgtcaggat
     4861 gaagatctcc ttttcggcct cggcgattcg cgccgagcgc tggatcgggc gcacggtgac
     4921 gaagtaggtg gcgaacgcga gcaggatgcc gacgacgatg ccggccagcg cggcgaggta
     4981 ggcatcggcg tgccagtacc cggccaccg
//
LOCUS       DASRWS010000577         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2241642, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000577 DASRWS010000000
VERSION     DASRWS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241642"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="mlaD"
                     /locus_tag="VFF72_10980"
     CDS             complement(<1..385)
                     /gene="mlaD"
                     /locus_tag="VFF72_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZV93732.1"
                     /translation="MEKKRVEILVGVFVLLGLLGIVFLALKAANLGTFSTADTYTLYA
                     KFDNIGGLKVRGPVRSAGVTVGRIASITLDPKTYQGVVRMEIDKKVQFPDDSSARILT
                     AGLLGDQYIGLEAGGSEKMFQPNDTI"
     gene            complement(415..1197)
                     /gene="mlaE"
                     /locus_tag="VFF72_10985"
     CDS             complement(415..1197)
                     /gene="mlaE"
                     /locus_tag="VFF72_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HZV93733.1"
                     /translation="MISLVGRLGRGTIDLLRVLGQHATFFVALVRACPAAMRRPFLVV
                     AQIHAIGNRSLVIILASGLAVGFVLALQTYYALVTYGASESLGLIVNLSLTRELGPVV
                     TALLFAGRAGTSLTAEIGLMKAGEQLAAMDMMAIDPKSRVLAPRFIAGIISMPILAIL
                     FSAVGILGAYVVAVPLIGVDAGNFWSIMQSGVDVRRDVGNGIVKSIVFGFICTFVALY
                     QGYDAEATPEGVAHGTTRTVVIASLAVLLTDFVLTALMFSTP"
     gene            complement(1220..2071)
                     /locus_tag="VFF72_10990"
     CDS             complement(1220..2071)
                     /locus_tag="VFF72_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93734.1"
                     /translation="MLQPGEAAAAGERANAQAMVEVDHVVFGYEGTSRTILNDVSLSF
                     DRGRVTAILGGSGCGKTTLLRLIGGVYPVARGEVRVDGHVVDARDAEQLFALRRRLGM
                     LFQFGALFTDLSVFENVAFPLREHTALDETMIRDIVLMKLNAVGLRGAARLRISEISG
                     GMARRVALARAIALDPELLLYDEPFAGLDPISMGVAANLIRTLNDTTAATSILVSHDV
                     EECFRICDYAYLMSSQGKVVAHGRPEQLAASEEPEVRQFIRGEPDGPVRFHYPAPPLA
                     QDFGVRA"
     gene            complement(2074..>2742)
                     /locus_tag="VFF72_10995"
     CDS             complement(2074..>2742)
                     /locus_tag="VFF72_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZV93735.1"
                     /translation="IHFAMEFLPQQNKVNAGEGVDRQIRADDRNVVVIGGGDTGSDCV
                     GTSNRHGAKSVTQFELMPMPPEHEDKPLVWPYWPTKLRTSSSHEEGCERAFAIATKEF
                     IGEAGKVKALKTVGVEWKGGSMVEVPGTEKVLPAELVLLAMGFVSPVATLLDAFGVEK
                     DSRGNARAHVDAVGGYATSVPRVFAAGDVRRGQSLVVWAIREGRQAARAVDEFLMGAS
                     ALPR"
BASE COUNT          498 a          903 c          905 g          436 t
ORIGIN      
        1 ggatcgtgtc gttcggctgg aacatctttt cggaaccgcc cgcctcgagc ccgatgtact
       61 gatcgccgag caggccggcg gtcaggattc tcgccgacga atcgtcaggg aactgcacct
      121 tcttgtcgat ctccatgcgg accacgccct ggtaggtctt gggatcgagc gtgatggacg
      181 cgatccggcc gacggtcacg ccggcgctgc gaaccggccc gcgcaccttc aggccgccga
      241 tattgtcgaa tttcgcgtag agcgtgtagg tgtcggcggt gctgaaggtt ccgagattgg
      301 cggccttcag agccaggaag acgatcccga gcaggcccag cagcacgaag acgccgacca
      361 gaatctcaac gcgtttcttt tccatcgatc gttttgtcca gcaaaggacg aatgctacgg
      421 tgtcgagaac atcagcgccg tcaggacgaa gtcggtgagc agcacggcga gcgaggcaat
      481 caccacggtt cgcgtcgttc cgtgcgccac gccctcgggc gtcgcctcgg cgtcgtagcc
      541 ctggtagagg gcgacgaagg tgcagatgaa gccgaagaca atgctcttga cgatgccgtt
      601 gccgacgtcg cgcctcacgt cgacacccga ctgcatgatg gaccagaagt tgcccgcatc
      661 gacgccgatc agcggcaccg ccaccacgta ggcgccgaga atgccgaccg ccgagaaaag
      721 gatcgccagg atcggcatcg agatgattcc ggcgatgaag cgcggtgcca ggacgcggct
      781 cttcggatcg atcgccatca tgtccatggc agcgagttgc tcgccggcct tcatcaggcc
      841 gatctcggcg gtaagcgacg tgccggcgcg acccgcgaag aggagcgctg tcaccacggg
      901 gccgagctct cgcgtcagcg agaggttgac gatcaggccg agcgattccg aggcgccgta
      961 agtcaccagc gcgtagtagg tctgcaacgc cagcacgaat ccgaccgcga ggccggaggc
     1021 gaggatgatg acgagcgaac ggttgccgat cgcgtggatc tgggcaacga cgaggaaggg
     1081 ccggcgcatt gccgccgggc acgcacgaac gagcgccacg aagaaggtcg cgtgctggcc
     1141 cagcacgcga agcagatcga tcgtgccgcg cccgaggcgg ccgacgagcg aaatcatagg
     1201 agagcggcgt gcggcgtcgt cacgcgcgca ccccgaaatc ctgcgccagc ggtggcgccg
     1261 ggtagtggaa tcgcaccggt ccgtcgggct cgccgcggat gaactgccgc acctcgggct
     1321 cctcggacgc cgcgagctgt tccgggcggc cgtgggcgac caccttgcct tgcgaagaca
     1381 tcagataagc atagtcgcag atccggaagc actcctcgac gtcgtgcgag acgaggatcg
     1441 aggtggccgc ggtcgtgtcg ttgagcgtgc ggatgaggtt ggcggcgacg cccatcgaga
     1501 tcggatcgag cccggcgaaa ggctcgtcgt agagaagcag ctccgggtcg agcgcgatcg
     1561 cccgcgccag cgcgacgcgc ctggccatgc cgccggatat ctccgagatc ctcagcctgg
     1621 cggcgccgcg cagtccgacc gcgttgagct tcatcaagac gatgtcgcgg atcatcgtct
     1681 cgtcgagcgc ggtgtgctcg cgcaacggga aggcgacatt ctcgaagaca gaaagatcgg
     1741 tgaaaagggc gccgaactgg aaaagcatcc cgaggcgtct gcgcagcgcg aacagctgct
     1801 cggcgtcgcg cgcgtcgacg acgtggccat cgacacgtac ttcgccgcga gcgacgggat
     1861 aaacgccgcc gatcaggcgc agaagggtcg tcttgccgca ccctgaaccg ccgaggatcg
     1921 ccgtcacgcg accgcgatcg aacgacaggg acacgtcgtt gaggatggtc cggctcgtcc
     1981 cttcgtagcc gaagacgacg tgatcgacct cgaccatcgc ctgggcgttc gcccgctcgc
     2041 ccgccgcagc ggcttcgccg ggttgcagca tggctagcgc ggcagggcgc tggcgcccat
     2101 caggaattca tcgacagctc gcgccgcctg ccgtccttca cggattgccc agacgacgag
     2161 cgactggccg cggcggacgt cgccggcggc gaagacgcga ggcaccgacg tcgcatagcc
     2221 gccgaccgcg tcgacgtgcg cccgggcatt gcctcgtgaa tccttctcga cgccgaaggc
     2281 atcgagaagc gttgccaccg gactcacgaa acccatcgcc agcagcacga gctcggccgg
     2341 caggaccttt tcggttccgg gtacctccac catcgagccg cccttccatt cgacgcccac
     2401 ggtcttcaag gccttgacct tgccggcttc gccgatgaac tccttggtcg cgatcgcgaa
     2461 cgcccgctcg caaccctcct cgtgactcga cgacgtacgc agcttggtcg gccagtatgg
     2521 ccagacgagc ggcttgtctt cgtgttcggg cggcatcggc atcagctcga actgcgtcac
     2581 gctcttcgcg ccgtgccggt tgctcgtccc gacgcagtcg ctgcccgtgt cgccgccgcc
     2641 gatgacgacg acattcctgt cgtcggcccg gatctgccga tcgacgcctt ccccggcgtt
     2701 gaccttgttt tgctgcggca ggaactccat cgcgaaatgg at
//
LOCUS       DASRWS010000578         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3677779, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000578 DASRWS010000000
VERSION     DASRWS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677779"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VFF72_11000"
     CDS             <1..1311
                     /locus_tag="VFF72_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZV93736.1"
                     /translation="DFRRLVTGEPDIGEMIMRAFILRRVGLLRHAQGGVVLIGPGHSA
                     DTLRLEQFLTRNAYPHRLIDTEHDEGASGFLACYSIAPAELPVVVVPGQRAWRNPPLA
                     TLADDLGLTEKFDHESVFDVAVVGAGPAGLAAAVYAASEGLQTIIVESLAPGGQAGTS
                     SRIENYLGFPTGISGQALAGRAQVQAQKFGARLAVSRAATGLVCGDSPMRLRLEDDAV
                     IRSRAVIVATGARYRKLDVAGYERFEGQGIYYAATAMEADLCRRAAVVVVGGGNSAGQ
                     AAIFLAGVTTHVYMLVRGSGLAATMSDYLVQRIEGSSHISLHPFCEIIGLAGGDALEA
                     VTWRDRRDGAETRQAVVAVFAMIGAEPNTRWLGDCLQLDPRGFVVTGTKAGAAADSPY
                     STSRPGVYAVGDVRSGSVKRVASGVGEGSVVVLAVHRFLHPAPE"
     gene            complement(1425..>2237)
                     /locus_tag="VFF72_11005"
     CDS             complement(1425..>2237)
                     /locus_tag="VFF72_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93737.1"
                     /translation="FYWEIGDQTAALASGSVDSSTDPTVYTATTPMSIASASKWLYAS
                     YVVQRQAGVLSDSDVRFLNFTSGYTSFTSCQPGQTVQQCVDSGTNGVYSPLTDGRFDY
                     GGGHMEKHASLIGLGPLDDAGLAAEIESQLGSDVELAYSQPQLAGGVVTSADNYASFL
                     RKVLAGTLLMHDALGTHAVCTNPDTCPGAVGGPAPADESWHYSIGHWVEDDPTVGDGA
                     FSSAGSFGFYPWIDAPKTYYGIIARTASSGSGVDSVMCGRLIRKAWATAVAQ"
BASE COUNT          323 a          793 c          772 g          349 t
ORIGIN      
        1 gacttccgcc gtctcgtgac gggcgagccc gacatcggcg agatgatcat gcgagccttc
       61 atcctgcgcc gcgtcggcct gctgcgccac gcccagggcg gcgtcgtcct gatcggcccg
      121 ggacacagcg ccgacaccct gcgcctcgag cagttcctca cccgcaatgc ctacccgcac
      181 cgcctcatcg acaccgagca cgacgagggc gccagcggat tcctcgcctg ctactcgatc
      241 gcgccggcgg aactgccggt ggtcgtcgtc cccggccagc gcgcatggcg caacccgccc
      301 ctcgcgacgc tcgccgacga cctcggcctc accgagaagt tcgaccacga atcggtcttc
      361 gacgtcgccg tcgtcggcgc cggaccggcc ggtctcgccg ccgccgtcta cgcggcctcc
      421 gagggcttgc agaccatcat cgtcgagagc ctggcgccgg gcggccaggc cggcaccagc
      481 tccaggatcg agaactatct cggctttccg accggcatca gcggccaggc cctggccggc
      541 cgggcccagg tgcaggcgca gaagttcggc gcccgcctcg ccgtgtcgcg cgcggcgacc
      601 ggcctcgtat gcggcgactc gcccatgcgc cttcgtctcg aggacgatgc cgtcatccgc
      661 agccgcgcgg tgatcgtcgc cacgggagcg cgttatcgca agctcgacgt cgccggctac
      721 gagcgcttcg agggccaggg catctactac gcggccaccg cgatggaggc cgacctgtgt
      781 cgcagggccg ccgtcgtcgt cgtcggcgga ggcaattcag cgggccaggc ggcgatcttt
      841 ctcgccggcg tcaccacgca cgtgtacatg ctggtccgcg gcagcgggct ggcggcgacc
      901 atgtccgact atctcgtgca acgcattgaa ggctcgtcac acatcagcct gcatccgttc
      961 tgcgagatca tcggcctggc cgggggcgat gcgctcgaag ccgtcacctg gcgcgaccgg
     1021 cgcgacggcg ccgagacacg ccaggccgtc gtggccgtct tcgcgatgat cggcgcagag
     1081 ccgaacaccc gctggctcgg cgactgcctg caactcgacc cgcgcggctt cgtcgtcacc
     1141 ggcacgaagg caggagcggc ggccgattcg ccctactcga catcgcgacc gggagtctac
     1201 gcggtcggcg acgtccgctc ggggtcggtg aaacgggtgg cgtcgggggt cggcgaaggc
     1261 tcggtcgtcg tcctggccgt gcaccgcttc ctgcaccccg cgccggagta gaaaaggcgc
     1321 gtctgcgcgc agttcgttgc cgcggcggcg gcaacgaagg ccttcggagg caggcgaagc
     1381 ggttctgcgt tctcgtcagc tcgaagcgag gctccgattc cggactattg cgccaccgcc
     1441 gtcgcccagg ccttgcggat gaggcggccg cacatcaccg aatcgacgcc gctgccgctg
     1501 ctcgcggtgc gcgcaatgat cccgtagtac gtcttcgggg catcgatcca tggatagaag
     1561 ccgaaggagc cggcgctgct gaaggcgccg tcgcccacgg tcgggtcgtc ctcgacccaa
     1621 tgaccgatcg agtagtgcca gctctcgtct gccggcgccg ggccgccgac cgcgccgggg
     1681 caggtgtcgg ggttggtgca gaccgcatgc gtgccgagcg catcgtgcat cagcaacgtg
     1741 cccgcgagga ccttgcgcag gaagctggcg tagttgtcgg cgctcgtgac gacgccgccg
     1801 gcgagctgcg gctgcgaata ggcgagctcg acgtcgctgc cgagctgcga ttcgatctcg
     1861 gcagccagcc cggcgtcgtc gagcgggccg aggccgatca ggctggcgtg cttttccatg
     1921 tggccgccgc cgtagtcgaa gcggccgtcg gtgagcggcg agtagacgcc gttcgtgccg
     1981 ctgtcgacgc actgctgcac ggtctggcca ggctggcagg acgtgaagct cgtgtagccg
     2041 ctggtgaagt tcaggaacct gacgtcggag tcactcagca cgccggcctg cctttgcacg
     2101 acgtagctcg cgtaaagcca cttcgacgcc gaggcgatgc tcatcggcgt cgtcgcggtg
     2161 tagaccgtcg gatcggtgct gctgtcgacc gagcccgagg ccagcgcggc cgtctggtcg
     2221 ccgatctccc agtagaa
//
LOCUS       DASRWS010000579         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1690738, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000579 DASRWS010000000
VERSION     DASRWS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690738"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFF72_11010"
     CDS             <1..213
                     /locus_tag="VFF72_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZV93738.1"
                     /translation="LPQFIDPARSLWLQFVVMAGTFAVLEMITEVTIAGLAHRVGPWL
                     RRVGKRFNRACGGLFVAIGVALPLRG"
     gene            228..1232
                     /locus_tag="VFF72_11015"
     CDS             228..1232
                     /locus_tag="VFF72_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZV93739.1"
                     /translation="MTARHDPLSDVLRSVNLRGKVFYYVSCRDDWVAETPPSSELALA
                     LNPGVEHMLAYHMIIRGSGWAAVAGEAPVRIAGGDIVMFPRGDAHVLSSAPGMHSHGD
                     DSEWRITTRDDPKPIAVAFHDGVCDPGAELPASDAGTVFICSFVSCDLRPFNPLIHAL
                     PRLMHLSASGVGAWVAPMLAQAVSESRERRPGSAALLERVSEMVFVDAARRHLESLPD
                     GGGSGWLAALRDRHVGRAIGLLHERPAHEWTVEELGREVGMSRSALHERFVEMVGQPP
                     MQYLTNWRMQLGAKALRESRATVATIAQDVGYDSEAAFSRAFRRLMGEPPAAWRRAHA
                     "
     gene            1424..2257
                     /locus_tag="VFF72_11020"
     CDS             1424..2257
                     /locus_tag="VFF72_11020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV93740.1"
                     /translation="MSHVIDTPAAAPDYAAIKQRQQATWASGDYALIGATLQIVGETL
                     AEAADVRAGERVLDVAAGNGNATLGAARRFAEVTSTDTVQALLDKGRERARADGLQVK
                     FQIADAESLPFADGSFDVVLSTFGVMFTPQHARSASEMLRVVRKGGRIGLANWTPDGF
                     IGRLFKVIGTHVPPPAGLKSPALWGMEPHMVELFGTQAAQIRCERRNFVFRYRSAAHW
                     LQMFRDYYGPMLKAFAALGESARPALERDILALLESLNVGGKNTLIIPGEYLEVVVVK
                     A"
     gene            2303..2869
                     /locus_tag="VFF72_11025"
     CDS             2303..2869
                     /locus_tag="VFF72_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93741.1"
                     /translation="MALERARLLFVGLLSLGMAGIGVWMLVSGKGWHDKAWGTAGVIF
                     FGLGGGMLLHSATKDELSLTRQVRIPTRTRPFVIPAALLVSLPWSQKFSVCLPLLVAW
                     AALLPRYENVRTLRAACGLAVVIAAGQAFLAVMAATGTDPTESPEALVPQLVFSGLVV
                     LIDIRVLWEVYKRLRSSSATQGLPSYNP"
     gene            complement(2909..>4494)
                     /locus_tag="VFF72_11030"
     CDS             complement(2909..>4494)
                     /locus_tag="VFF72_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HZV93742.1"
                     /translation="LRLQGRDAEIARDILAELQARLSFLQEVGLAYLSLDRSAPTLSG
                     GEAQRIRLAAQLGSNLQGVAYVLDEPTIGLHPRDNAILLDALQNLADKGNTLVVVEHD
                     EETIRRADHIIDIGPGAGKRGGRLVAEGSVSEISSHADSLTGRFLAHPLVHPVQPRRP
                     VAEVTAATMRKEGADNALLTICDATLHNLRNVTVDIPLKRLVAITGVSGSGKSTLARD
                     VLLANLQFINTRGARPNWQGCEKIDGYAWIDRVLEVDQTPIGKTPRSCPATYIGFWDT
                     IRKLFAETLEAKARGYGPGRFSFNIGEGRCAACEGQGVRTIAMSFLPDVKVPCEVCHG
                     ERFNPETLAVSWRGKSIGDVLKMEVDEAVDFFSTMPNISHPLQLLKDVGLGYLTLGQP
                     SPTLSGGEAQRIKLVTELSKVRDDVTRRGNKVPHTLYVLDEPTVGLHMADVERLIRVL
                     HRLVDGGHSVVVIEHDLDVIAEADWVIDLGPEGGVNGGRVVAEAVPERLVAIARSHTG
                     AALGPVLSRAPSGEALEAA"
BASE COUNT          638 a         1598 c         1547 g          711 t
ORIGIN      
        1 ctgccgcagt tcatcgaccc ggcacgcagc ctctggctgc agtttgtggt catggccggc
       61 accttcgccg tgctcgagat gatcaccgag gtgacgatcg cgggcctcgc ccatcgcgtc
      121 ggtccctggc tgcgacgcgt cggcaagcgc ttcaaccgcg cctgcggcgg cctgttcgtg
      181 gcgatcggcg tggccctgcc gctgcgcggc tgagcggcgc tcctggcatg acggcacggc
      241 acgacccctt gtccgatgtg ctgcgcagcg tcaacctgcg cggcaaggtg ttctactacg
      301 tcagctgccg cgacgactgg gtggccgaga cgcccccgtc gtccgagctg gcactggcgc
      361 tcaaccccgg cgtcgagcac atgctggcgt accacatgat cattcgcggc agcggctggg
      421 cggccgtggc aggcgaggcg ccggtgcgca tcgccggggg cgacatcgtc atgttcccgc
      481 gcggcgatgc ccacgtgctg tcgagcgcgc ccggcatgca ttcgcacggc gacgacagcg
      541 aatggcgcat caccacgcgc gacgacccga agccgatcgc cgtcgccttc cacgacggcg
      601 tgtgcgatcc cggcgccgag ctgccggcca gcgacgccgg cacggtcttc atctgcagct
      661 tcgtcagttg cgacctgcgg ccgttcaacc cgctcatcca cgcgttgccg cgcctcatgc
      721 acctgagcgc cagcggtgtc ggcgcctggg tcgcgccgat gctggcccag gccgtgagcg
      781 agtcgcgcga gcggcggccg ggcagcgcgg cgctgctcga acgcgtcagc gaaatggtct
      841 tcgtcgacgc ggcgcgccgg catctcgaat cgcttcccga cggtggcggc agcggctggc
      901 tggcggcgct gcgcgatcgc cacgtcggcc gcgccatcgg cctcctgcac gagcggcccg
      961 cgcacgaatg gacagtcgag gagctcggcc gcgaggtcgg catgtcgcgt tcggcgctgc
     1021 acgagcgctt cgtcgagatg gtcggccagc cgccgatgca atacctcacc aactggcgca
     1081 tgcagctcgg cgcgaaggcg ctgcgcgaaa gccgcgccac cgtggcgacg atcgcgcagg
     1141 acgtgggcta cgactccgag gccgccttct cgcgagcgtt caggcggctg atgggcgagc
     1201 cgcccgccgc gtggcggcgc gcgcacgcgt aacgccagcc tgccggcacg cgcctcgagc
     1261 gggcagcgcg attcggactg ccgagcaaat cctccggacg caggggaaag ccccgttcgt
     1321 ccgcgccgaa aagaattccc tccgtcgcca cgaccgcgtg gcgatgcccg acgcggccct
     1381 tcacggaccc cgcgcaggtc gtccaccttc tccggagcct ctcatgagcc acgtcatcga
     1441 tacccccgcc gccgcacccg actatgccgc catcaagcaa cgccagcagg ccacctgggc
     1501 cagcggcgac tacgcgctga tcggcgcgac cctgcagatc gtcggcgaga cgctcgccga
     1561 ggccgccgac gtgcgggccg gcgagcgcgt gctcgacgtc gccgccggca acggcaacgc
     1621 gacgctcggc gcggcccggc gcttcgccga ggtcacctcc accgacaccg tgcaggcgct
     1681 gctcgacaag ggtcgcgagc gcgcccgggc cgacgggctc caggtgaagt tccagatcgc
     1741 cgatgccgaa tcgctgccct tcgccgacgg cagcttcgac gtcgtgctgt cgaccttcgg
     1801 cgtgatgttc acgccgcaac acgcgcgcag cgccagcgag atgctgcgcg tcgtgcgcaa
     1861 gggcggccgc atcggcctgg ccaactggac acccgacggc ttcatcggcc ggctgttcaa
     1921 ggtgatcggc acgcacgtgc cgccgccggc cggcctgaag tcgcccgcgc tctggggcat
     1981 ggaaccgcac atggtcgagc tgttcggcac gcaggcggca cagatccgct gcgagcgccg
     2041 taacttcgtc ttccgctacc gctcggccgc gcactggctg cagatgttcc gcgactacta
     2101 cggaccgatg ctcaaggcct tcgccgccct cggcgaaagc gcccggccgg ccctggaacg
     2161 cgacatcctc gccttgctcg aaagcctgaa tgtcggcggc aagaacacgc tgatcattcc
     2221 cggcgaatat ctcgaggtcg tcgtcgtcaa ggcgtgacgt cgtaccggtg gcggtcgccg
     2281 cccgcgggcg acaatggcca tcgtggcgct cgaacgtgct cgcctcctct tcgtcggcct
     2341 cctgagcttg ggcatggccg ggattggcgt gtggatgctc gtcagtggca aggggtggca
     2401 cgacaaggcg tggggcactg ccggcgtcat cttcttcggc ctcggcggag ggatgcttct
     2461 gcactccgcc acgaaagatg agctctcgct gacgaggcag gtccggatcc cgacgcggac
     2521 ccggcctttc gtcataccgg ccgctctgct tgtcagcctg ccatggtcgc agaagttctc
     2581 ggtctgcctg cccctgctcg tcgcctgggc tgcgcttctt ccgcgctacg agaacgtgcg
     2641 cacgctgagg gcagcatgcg ggcttgctgt cgtcatcgcg gccggccagg cgttcctcgc
     2701 cgtgatggca gcgaccggaa cggatccgac cgaaagtccc gaagcgttgg tgccgcaact
     2761 cgtcttttcc ggcctcgtgg tgctgatcga catccgggtg ctgtgggagg tgtacaagcg
     2821 acttcgctcg tcttcggcaa cgcagggcct gccttcgtac aacccgtgac cgcgatgcgg
     2881 gcgcggaacg cgcccgacgc ggttcagctc acgctgcttc gagcgcttcg cccgacggcg
     2941 cacgcgacag cacgggcccg agcgccgcgc cggtatggct gcgggcgatt gcgacgagcc
     3001 gctcgggcac cgcctccgcc accacgcggc cgccgttgac gccgccttcg ggaccgaggt
     3061 cgatcaccca gtcggcctcg gcgatgacgt cgaggtcgtg ctcgatgacg acgacgctgt
     3121 gcccgccgtc gaccagccgg tgcaggacgc ggatgaggcg ctcgacgtcg gccatgtgca
     3181 ggccgacggt gggctcgtcg agcacgtaga gcgtgtgcgg caccttgttg ccacgccggg
     3241 tgacgtcgtc gcgcaccttc gagagctcgg tgaccagctt gatgcgctgc gcctcgccgc
     3301 ccgaaagcgt cggcgacggc tggccgagcg tgaggtagcc gaggcccacg tccttcagca
     3361 gctgcagcgg atgcgagatg ttgggcatgg tcgagaagaa gtcgaccgcc tcgtcgacct
     3421 ccatcttcag cacgtcgccg atgctcttcc cccgccagct cacggccagc gtctccgggt
     3481 tgaagcgctc gccatggcag acctcgcacg gcaccttcac gtcgggcagg aagctcatgg
     3541 cgatggtgcg cacgccctgc ccttcgcacg ccgcgcagcg gccttcgccg atgttgaagc
     3601 tgaaccggcc cgggccgtag ccgcgggcct tcgcctcgag cgtctcggca aacagctttc
     3661 ggatcgtgtc ccagaagccg atgtaggtgg ccgggcacga gcgcggcgtc ttgccgatcg
     3721 gcgtctggtc gacctcgagg acgcggtcga tccaggcata gccgtcgatc ttttcgcagc
     3781 cctgccagtt cggacgcgcg ccacgcgtat tgatgaactg caggttggcg agcagcacgt
     3841 cgcgcgccag cgtgctcttg cccgagccgc tcacgccggt gatggcgacg aggcgcttga
     3901 gcggaatgtc gacggtgaca ttgcgcaggt tgtgcagcgt cgcgtcgcag atcgtgagga
     3961 gcgcattgtc ggcgccttcc ttcctcatcg tcgccgccgt cacctcggcg accggccggc
     4021 gtggctgcac cgggtggacc agcggatggg cgaggaagcg gccggtgagc gaatcggcat
     4081 gcgaggaaat ctcgctgacg ctgccttcgg caaccagccg cccgccgcgc ttgccggcgc
     4141 cgggcccgat gtcgatgatg tggtcggcgc ggcggatcgt ctcctcgtcg tgctcgacga
     4201 ccacgagcgt gttgcccttg tcggcgaggt tctgcagcgc gtcgagcagg atggcgttgt
     4261 cgcgcggatg caggccgatc gtcggctcgt cgagcacgta ggccacgccc tgcaggttcg
     4321 agccgagctg cgccgccagc cggatgcgct gcgcctcgcc gcccgagagc gtcggcgccg
     4381 agcggtcgag cgacaggtac gcgaggccga cctcctgcag gaacgacagc cgggcctgca
     4441 gttccgccag aatgtcgcgc gcgatctcgg cgtcgcggcc ttgcagccgc agct
//
LOCUS       DASRWS010000580         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3105554, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000580 DASRWS010000000
VERSION     DASRWS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105554"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFF72_11035"
     CDS             complement(<1..1334)
                     /locus_tag="VFF72_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93743.1"
                     /translation="MEPRGDRLTAAHGFALAIALGIAILAGGCSSPSGIALDPAWASG
                     GVAVSRDLFGHNTVWSEGGLGLWDDGARALVPQAVQETQQLTPGLLRFPGGTRAMRYH
                     FAEARGATRTPQCDPFRGTTDATTYGPDEFLSLAVTLGADVTWVAPWVDGSPDESAAI
                     ASFVAATPAVKYLEIGNEPYLGLPAGPSSGSCGRPTQFVQDERWDGTTRIPTTAADYA
                     AQLAATAPKVRAANPALLIGAPATSQYDGTSDAMDAVGDVDALAGGDPWNARLLSDAG
                     DAFDFFVLHPYDFTVDDDARVRLAERARKTVRDLRAAAPTKQVGITEYGFLFGGGTLL
                     NAIVTADMTRMAIEERLLLCVRHILIETDASGPFADSAAIGPSLAEKPGWFASELVRR
                     LQPVALPTSADLAADGTLVALPTRDDAMTHLVVLFVDRRTDDGPARTVRLALP"
     gene            complement(1310..>2539)
                     /locus_tag="VFF72_11040"
     CDS             complement(1310..>2539)
                     /locus_tag="VFF72_11040"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HZV93744.1"
                     /translation="DGQRGGGSMSPGAALTLLVIFGIIVVIVMVTKKRGGDRAEVMVD
                     RSTQAEGLAALRGQDPGFDDAAFAERAKGVMAKVNEAWIAGAMGPARRHISDGVYVRF
                     QTQLQLLKAQGLRNAMADWSVVGCEVLAAESDAKWDTVHVKMVGQARDADVPANLSAP
                     QAMEKAKQAPLVRYEEVWSFLRKRGSKSKNGVPTLEGRCPNCGADLPVSDVVRCEYCK
                     AVVNSGEHDWVLAEITQPEEWRPWMTNADLEGLNALQAKDPAVSRQELEDRASVVFWK
                     WIDARVTGTRQRFERFCLQPGQPPADRAALSQVAVGSSELMDVTVAQDGPIGPVDRVE
                     VEIVWSASENGGQPVNQTSRVVLARATGATSKGGLNSLDCPNCRGPLADSDLAKCNFC
                     GEPLAGGKYEWSLEAIG"
BASE COUNT          355 a          929 c          899 g          356 t
ORIGIN      
        1 ggcaacgcca gacggacggt gcgcgccggg ccgtcgtcgg tgcggcgatc gacgaagagc
       61 acgaccaggt gggtcatggc gtcgtcgcgc gtgggcagcg cgaccagcgt gccgtcggcg
      121 gcgagatcgg ccgaggtcgg cagcgccacc ggctgcaggc ggcgcaccag ctcggaggcg
      181 aaccagcccg gcttctccgc cagcgacggg ccgatcgcgg ccgagtcggc gaagggtccc
      241 gaggcgtcgg tctcgatgag gatatgtcga acgcacaata agagccgctc ttcgatcgcc
      301 atgcgcgtca tgtcggcggt gacgatggcg ttgagcagcg tgccgccgcc gaagaggaag
      361 ccgtattcgg tgatgcccac ctgcttggtg ggcgcggcgg cgcgcaggtc gcgcaccgtc
      421 ttgcgcgcgc gctcggcgag gcggacgcgg gcgtcgtcgt cgacggtgaa gtcgtagggg
      481 tggagcacga agaagtcgaa cgcgtcgccg gcgtcggaca gaaggcgcgc gttccacggg
      541 tcgccgcccg cgagcgcgtc gacgtcaccg acggcgtcca tggcgtcgga cgtgccgtcg
      601 tattgcgacg tcgccggcgc gccgatcagc agcgccggat tggcggcgcg caccttcggc
      661 gccgtcgccg cgagctgcgc ggcgtagtcg gcggcggtcg tcgggatgcg cgtcgtgccg
      721 tcccagcgct cgtcctgcac gaactgcgtc ggacggccgc acgagcccga cgacggaccg
      781 gcgggcaggc cgaggtacgg ctcgttgccg atctcgaggt acttcaccgc cggcgtggcc
      841 gcgacgaacg acgcgatcgc cgccgactcg tcggggctgc cgtcgaccca cggcgcgacc
      901 caggtgacgt cggcgccgag cgtgacggcg agcgacagga actcgtcggg cccgtatgtg
      961 gtggcgtcgg tggtgccgcg gaacggatcg cattgcggcg tgcgcgtggc tccgcgcgcc
     1021 tcggcgaagt gatagcgcat cgcgcgcgtg ccgccgggga agcgcagcag gccaggcgtc
     1081 agctgctgcg tttcctgcac cgcttgcggc acgagcgcgc gcgcgccgtc gtcccacagg
     1141 ccgaggccgc cttcggacca gacggtgttg tgcccgaaga gatcgcgcga cacggcgacg
     1201 ccgcccgacg cccacgccgg gtcgagcgcg atccccgacg gcgacgagca gccgccggcg
     1261 aggatcgcga tcccgagcgc gatggcgagc gcgaagccgt gcgctgccgt cagccgatcg
     1321 cctcgaggct ccactcgtac ttgccgccgg cgagcggctc gccgcagaag ttgcacttgg
     1381 ccagatccga atcggcgagc ggcccacgac agttggggca gtcgagcgag ttgagcccgc
     1441 ccttggacgt cgcgccggtc gcgcgcgcca gcaccacgcg gctcgtctgg ttcaccggct
     1501 ggccgccgtt ctccgacgcc gaccagacga tctcgacctc cacccgatcg acgggaccga
     1561 tgggcccgtc ctgcgccacg gtcacgtcca tcagctccga gctgccgacg gcgacctgcg
     1621 acagcgccgc gcgatccgcc ggcggctgcc ccggctgcag gcagaagcgc tcgaagcgct
     1681 gccgcgtgcc ggtcacgcgc gcatcgatcc acttccagaa cacgacggac gcgcgatcct
     1741 cgagctcctg gcggctcacc gccggatcct tcgcctgcag cgcgttgagc ccttcgaggt
     1801 cggcgttcgt catccacgga cgccactcct cgggctgcgt gatctcggcc agcacccagt
     1861 cgtgctcgcc cgagttgacc accgccttgc agtactcgca gcgcaccacg tcgctcaccg
     1921 gcagatcggc gccgcagttg gggcagcgcc cctccagcgt gggcacgccg ttcttcgatt
     1981 tgctgccgcg cttgcgcagg aacgaccaga cctcttcgta gcgcaccagc ggcgcctgct
     2041 tggccttctc catcgcctgc ggcgcgctca ggttcgccgg cacgtcggcg tcgcgcgcct
     2101 ggccgaccat cttcacgtgc acggtgtccc acttggcgtc gctctcggcg gcgagcactt
     2161 cgcaaccgac gaccgaccaa tcggccatcg cgttgcgcag cccctgcgcc ttcaagagct
     2221 gcagctgcgt ctggaagcgc acgtacacgc cgtcggagat gtggcgccgc gccggtccca
     2281 tggcgccggc gatccacgcc tcgttgacct tggccatgac gcccttggcg cgctcggcga
     2341 aggccgcgtc gtcgaagccg ggatcctggc cgcgcagcgc cgccaaccct tcggcctgcg
     2401 tcgagcgatc gaccatgacc tcggcgcgat cgccgccgcg cttcttggtc accatgacga
     2461 tgacgacgat gatcccgaag atcaccagca gcgtcagcgc cgcgccgggc gacatcgagc
     2521 cgccgccccg ctgcccgtc
//
LOCUS       DASRWS010000581         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2243020, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000581 DASRWS010000000
VERSION     DASRWS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243020"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFF72_11045"
     CDS             complement(<1..1173)
                     /locus_tag="VFF72_11045"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HZV93745.1"
                     /translation="MNKHLHRLVFSSARGACIAVAETVASTGKAASGERRSGRHGAAA
                     TCALGLAIAAFGAQAQSVGGVGAAAGTFVTGVAPKASVQTGNVLPTGGVVVKGSGSIQ
                     VSGADMTINQASQRLVANWQSFSIGSGNSVRFVQPSSSAVALNRVTGDDASRIFGSLT
                     ANGHVYLENANGVYFAPGAQVSVGSLLATTLHVDDDQFMQGNLRLSGGSTSLGEVSNG
                     GSIVAAPGGTVILAGPVVSNTGAIVTPGGTTALVAANAVNVDPTGSGLLNISVPVAAV
                     NASLVQSGSIAADGGSVQLAAAATDAALRTVMQVDGVVRARSIESRNGSIVLSGGSSG
                     IVAVTGQLDVSGHAGMSGGTIEVLGERVGLMNEARLDASGSAGGGTVLVGGNRQGNG"
     gene            complement(1192..2943)
                     /locus_tag="VFF72_11050"
     CDS             complement(1192..2943)
                     /locus_tag="VFF72_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HZV93746.1"
                     /translation="MPHSNSTTSRSRRPALGGSLFLGLLIPLVALAQTPPQQPNAGSI
                     LQQVQPSLASPVPADRAAPDLRVPRAVAEPSSGGGAKVDVRGFRIAGLPEARADRLKP
                     LLQKYTGPGKTLADLEDAAKDIEVALQRQGLFLAQAYVPEQSLADGIVTIQVLEGRIG
                     AVKVEVEPGVRVAPEYLDRIVDVLRGHPVAERNLIERALFTLGDLRGIVVATTLSPGD
                     KVGEADLTVKISPGPKTAATVDLDNGGSLFTGRYRANVGLDWFSPTGRGDVASLRGQV
                     SDNLGSEFIRASWLTPINSLGTKVGIAASFLHYKLGSAIFEDLGAHGTAEDYALQLLH
                     PLIRSRNDNLFVQFSTNYRRFDDKVDSLPLDTKKDVSPYATVAVVGDFRDTFAGGAIS
                     NYSLGVVGGRLKINDEDDIVVDQQNYKSAGSYAKLQLGASRLQHLPTKDYLYLALNGQ
                     LASKNLDSSEKFSMGGPYGVRGYPSPESPSDSGLIATWEYRKQIPIEALPGDVVFSIF
                     GDYGVADLHHDPRTEADPTASAGGNIRRLVSHGIGVTYARRDGLTVKGYVAARGGLKA
                     QSDDSRAQLYLLVSQQF"
     gene            complement(3126..3815)
                     /locus_tag="VFF72_11055"
     CDS             complement(3126..3815)
                     /locus_tag="VFF72_11055"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HZV93747.1"
                     /translation="MLRLSARHWRGGVALGLGLPLLLGSGPAAAGGLGYGVVGLPTWL
                     GLVAALWALFGARMLPSSASGIVFALLALAMLGSAWWAWRRQQGSAISSANPVAARAA
                     PVVIPDGLDGAAVLDAARARFLRLQAAWDVADISTLKSLTTPEMLEELMHVLTARGAG
                     TSRTDVITLHAELLALEELSAAYLASVEFSGMIRESAERGPVPFRELWMLACMKDDGA
                     SWRLARQQSLF"
     gene            complement(3809..4540)
                     /locus_tag="VFF72_11060"
     CDS             complement(3809..4540)
                     /locus_tag="VFF72_11060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV93748.1"
                     /translation="MGETHFGYSSVDESAKAARVRGVFDSVAPKYDLMNDLMSLGLHR
                     AWKAYAAAVANVRPGDKVLDLAGGTGDMARAFAPRAGESGMVVHTDINEAMLRQGRDR
                     LLDEGIVLPTVLCDAESLPFATASFDLVSVAFGLRNMTRKEKALAEMARVLRDGGRLL
                     VLEFSRVAPPLERLYDFYSFQVLPRLGQWVAGDGESYRYLAESIRVHPSQAELKAMMK
                     SAGFAHVDVHNLAAGVVALHVGIRC"
BASE COUNT          757 a         1569 c         1572 g          704 t
ORIGIN      
        1 gccgttgcct tggcggttgc cgccgacgag gacggtgccg ccgccggcgc tgccactggc
       61 gtcgagacgc gcctcattca tcaggccgac gcgttcgccg agcacctcga tcgtgccccc
      121 cgacatgccg gcatgacccg acacatcgag ctggccggtc actgccacga tcccgctgct
      181 gccgcccgag agcacgatcg atccgttgcg gctttcgatg ctgcgcgcac gcacgacgcc
      241 gtcgacctgc atcacggtgc ggagtgctgc atcggtcgcc gccgcggcga gttgcacgct
      301 gccgccgtcg gcggcgatcg agccggattg caccagactg gcattgaccg ccgcgaccgg
      361 aacgctgatg ttgagcagcc ccgagccggt gggatcgacg ttgacggcgt tcgctgcgac
      421 cagcgccgtc gtgccgccgg gcgtgacgat ggcgccggtg ttcgagacca ccggcccggc
      481 caggatgacg gtgcctcccg gcgccgcaac gatcgatccg ccgttgctca cctcgccgag
      541 cgaggtgctg ccaccggaca gccgcaggtt gccctgcatg aactggtcgt cgtcgacgtg
      601 cagcgtcgtc gccagcaggc tgccgacgct cacctgcgcg ccgggcgcga aatagacgcc
      661 attggcgttc tcgagataga catggccgtt cgcggtgagc gagccgaaga tccggctcgc
      721 atcgtcgccg gtgacgcggt tgagcgcgac ggcgctgctc gacggctgca cgaagcgcac
      781 ggagttgccc gagccgatcg aaaaactctg ccagttggcg acgagtcgct ggctcgcctg
      841 gttgatcgtc atgtcggcgc ccgacacctg gatgctgccg ctgcccttga cgaccacgcc
      901 gcccgtcggc aggacgttgc cggtctgcac cgacgccttc ggtgcgacgc ccgtgacgaa
      961 cgtgccggcc gctgcgccca cgccgcccac cgattgcgcc tgcgcgccga aggcagcgat
     1021 ggcgaggccg agtgcgcagg tcgccgcggc gccgtggcga ccgcttcgcc gctcgcccga
     1081 cgctgccttg cccgtcgacg cgactgtttc ggcgaccgcg atgcaggcac cccgcgcgct
     1141 gctgaagacg agtcgatgca gatgcttgtt catgatggat cgccgtcgaa atcagaattg
     1201 ctggctgacc agcagataga gctgcgcacg gctgtcgtcg ctttgcgcct tcaggccgcc
     1261 gcgcgccgcc acgtaaccct tgaccgtcag cccgtcgcgg cgggcgtagg tgacgccgat
     1321 gccatgcgaa acgaggcggc ggatgttgcc gcccgcgctg gcggtcgggt cggcctcggt
     1381 gcgcggatcg tgatgcaggt cggcgacgcc gtagtcgccg aagatggaga agaccacgtc
     1441 accgggaagt gcctcgatcg ggatctgctt ccggtattcc cacgtcgcga tcaggcccga
     1501 gtcgctgggc gactcgggcg aagggtagcc acgcaccccg tagggaccgc ccatgctgaa
     1561 cttctcggag ctgtcgaggt tcttgctggc cagctggccg ttcagcgcga ggtagaggta
     1621 gtccttcgtc ggcagatgct gcaacctcga tgcaccgagc tgcagcttgg cgtagctgcc
     1681 cgccgacttg tagttctgct ggtcgacgac gatgtcgtcc tcgtcgttga tcttcaacct
     1741 gccaccgacg acgccgagcg aatagttgct gatcgcgccg ccggcgaacg tatcgcggaa
     1801 atcgccgacg acggcgaccg tcgcataagg cgagacatcc ttcttggtat cgaggggaag
     1861 cgaatcgacc ttgtcgtcga agcgtcgata gttggtgctg aactggacga acaggttgtc
     1921 gttgcgcgag cggatcagcg ggtgcagcag ctgcagcgcg tagtcttcgg ccgtgccgtg
     1981 cgcaccgagg tcctcgaaga tcgcggagcc gagcttgtag tggagaaaac tcgcggcgat
     2041 gccgaccttc gtgccgagcg aattgatcgg cgtcagccac gaagcgcgga tgaactcgga
     2101 gccgaggttg tcggagacct ggccacgcaa cgacgcgacg tcgccacgac cggtgggact
     2161 gaaccagtcg aggccgacat tggcccggta gcgaccggtg aagagcgatc cgccgttgtc
     2221 gaggtcgacc gtcgcggcgg ttttcggccc gggcgagatc ttcaccgtga gatcggcctc
     2281 gccgaccttg tcgccgggcg acagcgtcgt cgcgacgacg atgccgcgca ggtcgccgag
     2341 cgtgaacagg gcccgctcga tcaggttgcg ctcggccacg ggatggcctc gcaggacgtc
     2401 gacgatgcgg tcgaggtact cgggcgccac gcgcacgccg ggctcgacct cgaccttcac
     2461 cgcgccgatc cgaccctcga ggacctggat cgtgacgatg ccgtcggcca ggctctgttc
     2521 cggcacgtag gcctgcgcga gaaacagacc ttggcgttgc agggcgactt cgatgtcttt
     2581 ggcggcgtct tcgagatcgg ccagcgtctt gcccgggccg gtgtacttct gcaacagcgg
     2641 cttcagccga tcggcacgtg cctcaggaag cccggcaatg cgaaatcccc tgacatcgac
     2701 cttcgcgccg cctccggagc tcggctcggc cacggcacgc ggcacgcgca ggtcgggcgc
     2761 ggcacggtcg gcgggcacgg gcgaagcgag cgacggctgc acctgttgca ggatcgagcc
     2821 ggcgttgggt tgctggggcg gcgtctgcgc cagcgcgacc agcgggatga ggagaccgag
     2881 aaagagcgaa cccccgagcg cagggcgtcg cgaccgggac gttgtcgaat tggaatgagg
     2941 catggtcgat ggcagacgat gacgcggagg cgacatcgtc cgcggagagc gcgaccttac
     3001 gaacgcctca ccaccctgtc aatcgccccc atccgggcga cttgacgcga catcgccgct
     3061 ccgtagcctt cccatccgcg gtcggcctcg cattcagtgc gtctggaaga ctgcggagac
     3121 cgggctcaga acaacgactg ctgcctcgcc agtctccacg acgccccgtc gtccttcatg
     3181 cacgcgagca tccagagctc gcgaaagggc accggtcccc gctccgccga ctcgcggatc
     3241 attcctgaaa actcgacgct ggcgagatag gcggcgctca gctcttccag cgcgaggagt
     3301 tccgcatgca gcgtgatcac atccgtgcgg ctggtgcctg cgccacgtgc cgtcagcacg
     3361 tgcatcagct cttcgagcat ttcaggcgtg gtgagcgatt tcagggtcga gatgtcggcc
     3421 acgtcccagg cagcctgcag gcggaggaaa cgggcgcgcg ccgcgtcgag gaccgccgcg
     3481 ccgtcgagcc cgtctggaat gacgacaggt gccgcacgcg ccgcgacggg gttcgcggac
     3541 gagattgcgc tgccttgctg ccggcgccag gcccaccacg cgcttcccag catcgccagg
     3601 gcaagcaggg cgaacacgat gccgctggcc gacgatggca gcatgcgcgc gccgaacagg
     3661 gcccacaacg ccgccacgag gccgagccac gtcggcaggc cgacaacgcc atagccgagg
     3721 ccgcccgcgg ccgccggacc gctgccgagc agaagcggca agcccaagcc cagcgccacg
     3781 ccgccgcgcc agtgacgtgc cgacagcctc agcatcggat ccccacatgc agtgcgacga
     3841 cgccggcggc gaggttgtgc acgtcgacat gggcaaatcc cgccgacttc atcatcgctt
     3901 tgagctcggc ctgcgacgga tggacgcgga tcgactcggc gaggtagcga taactctcgc
     3961 catcgccggc gacccattgc cccagacgcg gcagcacctg gaaggagtag aagtcgtaga
     4021 gcctttccag cggcggcgcg acgcgcgaga actcgagcac cagaaggcgg ccgccatcgc
     4081 gcagcacgcg cgccatttcg gccagcgcct tctccttgcg ggtcatgttg cgcaggccga
     4141 aggcgacgct gacgagatcg aagctggcgg tggcgaaggg cagcgactcg gcgtcgcaga
     4201 gcacggtcgg caggacgatg ccttcgtcga gaagacgatc gcgaccctgt cgcaacatcg
     4261 cctcgttgat gtcggtgtgg accaccatgc cgctttcgcc ggcccgcggg gcgaaggcac
     4321 gcgccatgtc gccggtgcct ccggcgaggt cgaggacctt gtcacccggg cgaacattcg
     4381 cgaccgccgc cgcgtaggcc ttccaggccc gatgcaggcc gagcgacatc aggtcgttca
     4441 tcaggtcgta cttcggcgcg accgaatcga acacgccgcg cacgcgggcc gccttggccg
     4501 attcgtcgac cgatgaatag ccgaagtggg tctcgcccat ggccgcccgg gagccagcct
     4561 cagtggccgt ggccgcacga cgacgccgcg gcagccgcca tg
//
LOCUS       DASRWS010000582         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4245714, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000582 DASRWS010000000
VERSION     DASRWS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4245714"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFF72_11065"
     CDS             <1..633
                     /locus_tag="VFF72_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HZV93749.1"
                     /translation="YGTFACRSVDTLLVFGSDGRVYSAAVGVLPSGRGDGQPITSLVD
                     LQPGTQPSHFFAGDASQLLLLAGTGGFGLLAHAGDLVARQRGGKSFLTLEGDEKPLPP
                     AVVAAGAARVACLTLLGRLLVFGLDDVKLQAKGGRGLTLVDLDARDALVSVCVFAEGL
                     RVLGSGRGGKPKDEVLRGVALEAHVGKRARKGRRQDAMVRATRVLPVVPA"
     gene            734..970
                     /locus_tag="VFF72_11070"
     CDS             734..970
                     /locus_tag="VFF72_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93750.1"
                     /translation="MKKIVHDTTDAFDQRLDVLQAGHAPQVEIDAAIAVYERLRTAQS
                     ICQALVPGAYSESAMLALLEAISEEVAINTPIDD"
     gene            complement(1002..1487)
                     /locus_tag="VFF72_11075"
     CDS             complement(1002..1487)
                     /locus_tag="VFF72_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZV93751.1"
                     /translation="MSRSRGTAPALLVAVAIVSFAAVAIALVSQYAYAMDPCPWCVLE
                     RLLFVVIGLLALLGLAWRASAALRGIAGIAFLLACAGFAASIWQHFVAAKSASCNLTL
                     ADRIMGATQLDRLLPSVFEARASCADAAVTLAGIPYDFLAAAVFLACAVATLRILRGA
                     A"
     gene            complement(1520..1981)
                     /locus_tag="VFF72_11080"
     CDS             complement(1520..1981)
                     /locus_tag="VFF72_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV93752.1"
                     /translation="MPSIYDRLTDLDISLPPLAVPAGAYVPFVRSGNLVFLSGHIAKR
                     DGKAWTGQLGRDMTTSTGRKAARAVAIDLLGTLHAAVGDLDKIERIVRITSLVNSTGD
                     FTEHHLVTNGCSELLGEVFAEKGAHARSAYGVAQLPLGACVEIELVAQVGQ"
     gene            2046..>2234
                     /locus_tag="VFF72_11085"
     CDS             2046..>2234
                     /locus_tag="VFF72_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93753.1"
                     /translation="MRASAGGWIAASMLAWLAGVAVQLREPRLAPAAAYLAAALSALL
                     LLCVAAWSSRRAAPTWRRQ"
BASE COUNT          348 a          744 c          834 g          308 t
ORIGIN      
        1 tacggcacgt tcgcgtgtcg aagcgtcgac acgctgctcg tcttcggcag cgatgggcgc
       61 gtctattcgg cggcggtcgg cgtgctgccg agcgggcgcg gcgacggcca gccgatcacg
      121 tcgttggtcg acctgcaacc cgggacgcag ccatcgcact tcttcgcggg cgacgcgagc
      181 cagctgttgc tgctggccgg caccggcggc ttcgggctgc tcgcgcacgc cggagatctc
      241 gtggcccgcc agcgcggcgg gaagtccttc ctgacgctcg aaggcgacga gaagccgctg
      301 ccgccagcgg tcgtcgcagc gggcgcggcg cgcgtggcct gtctcacgct tctcggccgc
      361 ctgctcgtct tcggcctcga cgacgtcaag ttgcaggcca agggcggtcg cggcctcacg
      421 ctggtcgacc tcgacgccag ggacgcgctc gtcagcgtct gcgtcttcgc cgaaggcctg
      481 cgcgtcctcg gcagcggccg tgggggcaag ccgaaggacg aagtgttgcg cggcgtcgcg
      541 ctcgaggcgc acgtcggcaa gcgggcgcgc aagggccggc gccaggacgc gatggtgcgg
      601 gccacgcgcg tgctgccggt cgtgcccgcc tgagctgcag agatgggcga agcgtcgcgc
      661 tgcggtcgcc gggccgccgc cggatcgcga ctacgatcgc tctcttttcg cgaaccggat
      721 gccggagaag gccatgaaga aaatcgtcca cgacaccacc gacgccttcg atcagcggct
      781 cgacgtcctg caggcagggc atgcgccgca ggtcgagatc gacgccgcga tcgcggtcta
      841 cgagcggttg cgaacggcgc agtcgatctg ccaggcgctg gtgcccggcg cctactcgga
      901 atcggcgatg ctcgccttgc tcgaggcaat ttccgaagaa gtggcgatca acaccccgat
      961 cgacgactga acgatccggc ggcgcggccc tgagttcgcc ttcaggcagc gccgcgcagg
     1021 atccgcaagg tggccacggc gcaggccagg aagacggcgg cggcgaggaa gtcgtagggg
     1081 attccggcga gggtgacggc cgcatcggcg cagctggccc gggcctcgaa aaccgacggc
     1141 aaaagccggt cgagctgcgt ggcgcccatg atgcgatcgg caagggtgag gttgcatgag
     1201 gccgacttcg ccgccacgaa gtgctgccag atcgatgcag cgaagccggc gcaggccagc
     1261 aggaatgcaa ttccggcgat gccgcgcagc gccgccgacg cccgccaggc caggccgagc
     1321 agggcaagca ggccgatgac gacgaagagc agcctctcga ggacgcacca ggggcacgga
     1381 tccatcgcat aggcgtattg cgacacgagc gcgatcgcga cggccgcgaa ggaaacgatc
     1441 gccacggcca cgagcaaggc cggggcggtg ccgcgcgaac gcgacatggc gatcgatcgc
     1501 cgcgcagccg gggccgccat cactggccca cctgggcgac gagctcgatc tcgacgcagg
     1561 cgccgagcgg cagttgcgcc acgccgtagg cgctgcgcgc gtgcgcgcct ttctcggcga
     1621 acacctcgcc gagaagctcc gagcagccgt tggtcaccag gtggtgctcc gtgaaatcgc
     1681 cggtgctgtt gacgaggctg gtgatgcgca cgatgcgctc gatcttgtcg agatcgccga
     1741 ccgccgcgtg cagcgtgccg agcaggtcga tggcgacggc gcgcgccgcc ttgcgacccg
     1801 tcgaggtggt catgtcgcgg ccgagctggc cggtccaggc cttgccatcg cgcttggcga
     1861 tgtggcccga caggaacacg aggttgccgc tgcgcacgaa aggaacgtag gcaccggccg
     1921 gcaccgccag aggcggaagc gagatgtcga gatcggtgag gcggtcgtag atcgagggca
     1981 tggagaggat tcgctcggtc gcgagaaggg cgcaaatcgt acaccccgcc cctacactcc
     2041 ggacgatgcg agcatcggcg ggcggctgga tcgcggcgtc gatgctggca tggctggcgg
     2101 gcgtcgcggt tcagcttcgc gagccgcggc tggcgccggc ggcggcctat ctcgctgccg
     2161 cgctgtcggc gctgctgttg ctgtgcgtgg ccgcgtggtc gagccggcgc gccgcgccga
     2221 cgtggcgccg ccag
//
LOCUS       DASRWS010000583         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2821262, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000583 DASRWS010000000
VERSION     DASRWS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821262"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFF72_11090"
     CDS             complement(<1..1150)
                     /locus_tag="VFF72_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV93754.1"
                     /translation="MTDSRLLADEAAWCSFGDTVHYVDPPKVFERCEGSWLYDSSGVA
                     YLDLQMWYSAVNFGYANPRLGDAMKRQLDTLPQVASQYLHPTKIELARVIGEDALRKW
                     DRKGRVHFNVGGSQSIEDSLKLVRNARGGKSLMFAFEGGYHGRTLGASAITSSYRYRR
                     RFGHFGERAHFVPFPYHFRGSKGMGKEEYGEHCVAQFARLFETEYNGVWDPKTRQCEY
                     AAFYVEAVQGTGGYVIPPKNFFTGLKKVLDQYGVLLVVDEIQMGFYRTGKLWGIEHFG
                     VVPDVLVFGKALTNGLNPLAGLWAREDLISPEVFPPGSTHSTFASNPLGTAVGLETMR
                     MLAETDYETMVMKKGAHFLAGLKDLQKRHPEIGDVDGLGLALRAEICEA"
     gene            complement(1147..1884)
                     /locus_tag="VFF72_11095"
     CDS             complement(1147..1884)
                     /locus_tag="VFF72_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HZV93755.1"
                     /translation="MSGRLSRIDAGRCGWSVLCDFDGTIAQEDVTDSLLMRFGRPGWD
                     VLEVAWREGRIGSRECMAGQIALLDCSADELDEHLAGMAIDPDFARFATAVRRSGASL
                     TIVSDGLDRAIRNMLRRHDLDSLPVVASRLVATGPRRWKLEFPFASGACIAASSTCKC
                     AWAQPPHVAAGDRVLMVGDGASDFCVAGRADLSFASKRLLDHCLDHDLPHRAVANFRQ
                     ALELWPTLVASRAPGDEHSKPRSLPRP"
     gene            complement(1941..2216)
                     /locus_tag="VFF72_11100"
     CDS             complement(1941..2216)
                     /locus_tag="VFF72_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZV93756.1"
                     /translation="MTTQTLEAVAEVLAEKFGVEAARIRPEATLESLGLDSLTLMEFV
                     FAVEDRFSVRIPEERLDPRQAGVTLGHLSDLLEDALLTQLPADFRTA"
BASE COUNT          397 a          781 c          811 g          373 t
ORIGIN      
        1 cggcctcgca gatctcggcg cgcagcgcca ggccgaggcc gtcgacgtcg ccgatctcgg
       61 ggtggcgctt ctgcaggtcc ttcaggccgg cgaggaagtg cgcgcccttc ttcatcacca
      121 tcgtctcgta gtcggtctcg gcgagcatcc tcatcgtctc gaggccgacc gcggtgccga
      181 gcgggttgga agcgaaggtc gagtgcgtcg agccgggcgg aaagacctcg ggcgagatca
      241 ggtcctcgcg agcccacaga ccggccagcg ggttgaggcc gttggtgagc gccttgccga
      301 agacgagcac gtcgggaacg acgccgaagt gctcgatgcc ccagagcttg ccggtgcgat
      361 aaaagcccat ctggatctcg tcgacgacga gcagcacgcc gtactggtcg agcaccttct
      421 tcaggccggt gaagaagttc ttcggcggaa tgacgtagcc gccggtgccc tgcaccgcct
      481 cgacgtagaa cgccgcgtac tcgcactgcc gggtcttcgg gtcccagacg ccgttgtatt
      541 cggtctcgaa gaggcgcgcg aactgggcca cgcagtgctc gccgtattcc tccttgccca
      601 tccccttcga gccgcggaag tggtacggaa acggcacgaa gtgggcgcgc tcgccgaagt
      661 ggccgaagcg gcgccggtag cgatagctcg aggtgatcgc cgaagcgccc agggtccgcc
      721 cgtgatagcc gccttcgaag gcgaacatca ggctcttgcc gccgcgcgca ttgcgcacga
      781 gcttgagcga atcctcgatc gactgcgagc cgccgacgtt gaagtggacg cggccctttc
      841 gatcccactt gcgcagcgcg tcttcgccga tcacgcgggc gagctcgatc ttggtcggat
      901 gcaggtactg gctcgccacc tgcggcaagg tgtcgagctg gcgcttcatc gcgtcaccca
      961 ggcgcggatt ggcatagccg aagttgaccg ccgagtacca catctgcagg tcgagatagg
     1021 cgacgcccga gctgtcgtac agccagctgc cttcgcagcg ctcgaagacc ttgggcggat
     1081 cgacgtaatg gacggtatcg ccgaacgagc accaggcggc ctcgtcggca aggagacgcg
     1141 aatcggtcat gggcggggaa gggagcgggg tttcgaatgt tcgtcgcccg gcgcccggct
     1201 cgcaaccagc gtcggccaga gctcgagcgc ctgtcggaaa ttggcgacgg cgcggtgcgg
     1261 caggtcgtgg tcgaggcagt ggtcgagaag ccgcttgctc gcgaagctga ggtcggcccg
     1321 accggcgacg cagaagtcgg aggcgccgtc gcccaccatc aggacgcgat ctcctgcggc
     1381 cacgtgcggc ggctgcgccc acgcgcactt gcaggtgctg ctggcggcga tgcaagcccc
     1441 gctcgcgaac ggaaactcga gcttccagcg tcgcggcccg gtggcgacga gccggctcgc
     1501 caccaccggc aggctgtcga gatcgtgccg gcgcaacatg ttgcggatcg cccggtcgag
     1561 gccgtcgctg acgatcgtca ggctcgcgcc cgagcggcgc accgcggtgg cgaatcgggc
     1621 gaagtcggga tcgatcgcca tgcccgcgag gtgctcgtcg agctcgtcgg cgctgcagtc
     1681 gagcaaggcg atttgcccgg ccatgcattc gcgcgagccg atgcggcctt cgcgccatgc
     1741 gacttcgagg acgtcccagc cgggccggcc gaagcgcatc aggagcgaat cggtgacgtc
     1801 ttcctgcgcg atggtgccgt cgaagtcgca cagcaccgac caaccgcatc ggccggcatc
     1861 gatgcggctc aagcgacccg acacgccgaa gctccccatc gcagcgcccc gaacgaggcg
     1921 cggtccgcgc gacgcatcct tcaggccgtc cggaagtcgg ccggcagctg cgtcagtaac
     1981 gcgtcttcga gcaggtcgct caggtggccg agcgtgacgc cggcctggcg cgggtcgagc
     2041 cgttcctcgg ggatccgcac cgaaaagcga tcctcgacgg cgaacacgaa ctccatcagc
     2101 gtcagcgaat cgaggcccag gctttcgaga gtcgcctcgg gccggatacg ggccgcctcg
     2161 accccgaatt tctcggccag cacctcggcc actgcttcta aggtctgcgt ggtcatgatc
     2221 gaggcaggga caggggacaa ccgctcgggc tggtttgaaa ttcgcgacgg tgacgtatcc
     2281 tagccgaggg ttatcccatg cggatcctga ctcctgcctg accgcgatgc gtatcctcct
     2341 gatcgaagac gacgacgtgc tg
//
LOCUS       DASRWS010000584         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2822614, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000584 DASRWS010000000
VERSION     DASRWS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2822614"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1765
                     /gene="boxC"
                     /locus_tag="VFF72_11105"
     CDS             101..1765
                     /gene="boxC"
                     /locus_tag="VFF72_11105"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HZV93757.1"
                     /translation="MDQPAPPRVDYRTDPSRYRHWRISTEGVLARLTLDIAEDGGIAP
                     GYKLKLNSYDLGVDIELRDALDRIRFEHPQVRSVIVTSGKDRVFCSGANIFMLGVSSH
                     AWKVNFCKFTNETRNGIEDTSLHSGIKFVAAVNGACAGGGYELALACDEIVLVDDRSS
                     SVSLPEVPLLGVLPGTGGLTRVTDKRHVRHDLADIFCTSVEGVRGQRAVDWRLVDAVA
                     RPAQFAQAVHDRADRLATGSPRPLHAEGVSLPRLEREDAADAIRYAHVEVAIDRSRRT
                     AALTVKAPSGAQPADVAGIEAAGATWWPLAMARQLDDAILHLRTNELEIGTWLLKTEG
                     DSAAVLGADKTLLAHRGHWFVRETIGMLRRTFARLDVSSRSLFALIEPGSCFAGILAE
                     LAFAADRAYMLALPDDPARAPKLQLDDFNFGMLPLVDGQSRLARRFYGNEAELAAAND
                     TIGDALDADEALKLGLVTAAPDDIDWSDEIRIALEERAAMSPDALTGLEANLRFAGPE
                     NMATRIFGRLTAWQNWIFQRPNAVGARGALKVYGTGEKAGFDVHRV"
     gene            1901..>2151
                     /locus_tag="VFF72_11110"
     CDS             1901..>2151
                     /locus_tag="VFF72_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HZV93758.1"
                     /translation="MSIDYSQKIPNNVNLADDRTLQRALEQWQPNFIQWWDDVGPEGS
                     TNHDVYLRTATSVDPQGWAHFDYVKMRDYRWGIFLQPNDA"
BASE COUNT          341 a          795 c          668 g          347 t
ORIGIN      
        1 caacatgaag caaactgcac gtcgcgcttg atccgattct acacatgcat tatcatgcat
       61 tgatgttgcc ggccaacggc agcccatccc ggagttcctg atggatcagc ccgccccgcc
      121 ccgtgtcgac taccgcaccg atccttcgag gtaccggcac tggaggatct cgaccgaagg
      181 ggtgctcgcc cgcctcacgc tcgacatcgc cgaagacggc ggaatcgccc cgggctacaa
      241 gctcaagctc aacagctacg acctcggtgt cgacatcgag ttgcgcgacg cgctcgaccg
      301 catccgcttc gagcatccgc aggttcgctc ggtgatcgtc accagcggca aggatcgcgt
      361 cttctgctcg ggcgccaaca tcttcatgct cggcgtctcg agccacgcct ggaaggtgaa
      421 cttctgcaag ttcacgaacg agacgcgcaa cggcatcgag gacacgtcgc ttcattccgg
      481 catcaagttc gtcgccgccg tcaacggcgc ctgcgccggc ggcggctacg agctggcgct
      541 ggcttgcgac gagatcgtcc tggtcgacga tcgatcgtcg tcggtctcgc tgcccgaggt
      601 gccgctgctc ggcgtgctgc ccggtaccgg tggcctgacc cgcgtcaccg acaagcgcca
      661 tgtccgccac gacctcgccg acatcttctg caccagcgtg gagggcgtgc gtggccagcg
      721 cgcggtcgat tggcggctgg tcgatgcggt ggccaggccg gcgcaattcg cccaggcggt
      781 gcatgaccgg gccgaccgtc tcgccaccgg cagcccgcgt cccttgcatg ccgagggcgt
      841 gtcgttgccg cgtctcgagc gcgaagacgc cgccgacgcc atccgctatg cgcacgtcga
      901 agtcgccatc gaccgaagca ggcgcactgc cgccctgacc gtgaaggcgc cgagcggcgc
      961 acagccggcc gacgttgccg gcatcgaagc ggccggtgcc acgtggtggc cgctcgccat
     1021 ggctcgccag ctcgacgacg cgatcctgca cctgcgcacc aacgagctcg agatcggcac
     1081 ctggctgctg aagaccgaag gcgactccgc tgccgtgctc ggcgccgaca agacgctgct
     1141 cgcccatcgt gggcactggt tcgttcgcga gacgatcggc atgctgcgcc gcaccttcgc
     1201 gcggctcgac gtttcctcgc gttctctctt cgcgctgatc gagcccggct cgtgcttcgc
     1261 cggcatcctc gccgagctcg cgtttgccgc cgaccgcgcc tacatgctgg cgttgcccga
     1321 tgacccagcg cgcgcgccga agctgcagct cgacgacttc aacttcggca tgctgccgct
     1381 cgtcgatggc cagtcgcgcc tggcgcggcg cttctacggc aacgaagccg agctcgccgc
     1441 cgcgaacgac acgatcggcg acgcgctcga cgccgacgag gccctgaagc tcggccttgt
     1501 caccgcggcg cccgacgaca tcgactggag cgacgagatc cgcatcgccc tcgaggagcg
     1561 cgcggcgatg agccccgacg ccctcaccgg cctcgaggcg aacctgcgct tcgccggccc
     1621 ggagaacatg gccacgcgca tcttcggccg cctcaccgcc tggcagaact ggatcttcca
     1681 gcgtcccaac gccgtcggcg ccaggggcgc gctcaaggtc tacggcaccg gcgagaaggc
     1741 cggcttcgat gtgcatcgcg tctgagcagg ccgtgcggcc gctgcgcctc ttcatcttgc
     1801 caccgtggag cgcgccacgc gtccgtgcag ccgcgccttt cttggcggct tgacgccgtt
     1861 cgccgcccta ccgtcgcccc ggtccgtcag gagacatcac gtgtccatcg attactcgca
     1921 gaagatccct aacaacgtca acctcgccga cgatcgcacc ctgcagcgcg cgctcgagca
     1981 gtggcagccg aacttcatcc agtggtggga cgacgtcggc cccgagggct cgacgaacca
     2041 cgacgtctac ctgcgcaccg cgaccagcgt cgatccgcag ggctgggccc acttcgacta
     2101 cgtgaagatg cgcgactatc gctggggcat cttcctgcag cccaacgacg c
//
LOCUS       DASRWS010000585         9035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3425259, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000585 DASRWS010000000
VERSION     DASRWS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3425259"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFF72_11115"
     CDS             complement(<1..516)
                     /locus_tag="VFF72_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZV93759.1"
                     /translation="MRGRGLVDGARRLFPLAWPVFVGQIAVIAFSTVDTVMAARASAD
                     DLAALAIGAAAYISIFVGFMGIVLAVGPIAGQLYGAGRLDESGREMQQAMWLGLALSL
                     IGCALLLLPEPFLDLAQARGVVAEKVRAYLQGLAFALPPALVFTAYRGFNIAVSRPKA
                     VMILQIGALALK"
     gene            complement(513..971)
                     /locus_tag="VFF72_11120"
     CDS             complement(513..971)
                     /locus_tag="VFF72_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HZV93760.1"
                     /translation="MAALHIIDIESAINWWRDRYPSPDGITACPEVLALAEVYALMVY
                     YHESECDETSMPAAAHAAWLDWYATMPDAPCIAICSTSQGDSICKGCGRTFEEVRHWT
                     EMSPGEKRATWRRITNENTAWRFNRYADRAREGGTGADPKAVHDGDAAAA"
     gene            complement(1045..2922)
                     /locus_tag="VFF72_11125"
     CDS             complement(1045..2922)
                     /locus_tag="VFF72_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93761.1"
                     /translation="MLSRFTGAVASAARAALVALAPRAAFSVLVIGTGLAGCGPEDSG
                     SNAPVASVSLAATTAVPLGSTTRLAAQAMDAQGHAVSDKTASWNVADTTIAQVAGDGT
                     VTPLRVGFTIATASVDGITATTTLSVVGPLPLPTRSQYVGMNLSGIAYWTTEFPFTDL
                     MKNGGGWSSRYDNGTWGGTFPSMQGGYPTALNPNQHALYAVAWANTHYAAGQYVVLWD
                     GDGDISFPLSNVKVISSAPHRIVLDVSDTSGEMWVGIDRTNAADPVRNVRFLWPGTES
                     TYATQPFNLEFLKKVAPFSVLRFMDWGATNGSPVVEWADRSHVGDVTYANANGVPLEV
                     MIDLANTLHVDPWFCVPHQASDDYVRQFATLLHGRLDPALHPHIEYSNEVWNTGFAQT
                     TWALAQSTRLGLPQPWGTPSAFYAQRSVQIFKIFQQVYGPADSTRLVRVIAGQAAWTQ
                     FLENALAWQDTAANADVLAIAPYFNAAGTSDTTKVDATLQMTSDQVVDQMLVSIRGNI
                     TSWIKANAALAAKYHLTMKAYESGAGDSSSYFPADKQDAMTALFSAAHRNPRMKDVYD
                     EYYSLWIANGGQTMNQFNDAGGWSKWGLWGALEYVTEDAAAAPKYQGLLNVIAKHPAT
                     P"
     gene            complement(3144..3608)
                     /locus_tag="VFF72_11130"
     CDS             complement(3144..3608)
                     /locus_tag="VFF72_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93762.1"
                     /translation="MKIRSIPSTRGGWSPIVFAGALAGALACPWAMAQGSSGSAMTPQ
                     AQYAHDRAACESGRTTEDKATCLKEAGAALDARRHDQLTSDDTPLRNATDRCRMLPRK
                     DQADCVARIVGPLTPNQRVTVSGSVAGGGDIKETTTTTPGSVIVVVPEAPKK"
     gene            3789..4325
                     /locus_tag="VFF72_11135"
     CDS             3789..4325
                     /locus_tag="VFF72_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZV93763.1"
                     /translation="MKKPSVESQLFEALAFAAHKHRAQRRKDVEASPYINHPIALART
                     LAVEGGVTDTRTLIAAVLHDTVEDTDTTFEEIRAMFGAKVADVVREVTDDTKLDKAER
                     KRLQIEHAPHLSRRATLVKLADKTANLRDVASNPPRGWPLERKRAYFDWAKAVVDGLP
                     PVNKRLRAAFDAAFSQRP"
     gene            complement(4385..4633)
                     /locus_tag="VFF72_11140"
     CDS             complement(4385..4633)
                     /locus_tag="VFF72_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93764.1"
                     /translation="MHDELGLLQSLGFTLPSPAYIVGVILFGLIGMVAFYIGRHQSRP
                     RIKWLGVALMFYPYVVPSTWLMYAIGVALSVGVWMDRT"
     gene            4628..5167
                     /locus_tag="VFF72_11145"
     CDS             4628..5167
                     /locus_tag="VFF72_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZV93765.1"
                     /translation="MHRRHRHPCPSCRGCAARGFSLLELMVVIAVIAILALIALPGLP
                     DKFVRDQIVEAVKLADLAKGPVAAAWASHVALPADNAAAGLPAANLIVNNEISSVAIE
                     SGAIQVTFGNHANGNIRGKVLSLRPGVVESAPMVPVAWVCGNAEPPLNMIAKGPNRTD
                     VPLRFLPLNCRASAASAPG"
     gene            5175..6446
                     /locus_tag="VFF72_11150"
     CDS             5175..6446
                     /locus_tag="VFF72_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZV93766.1"
                     /translation="MPDGSGIVAAGLESARLRSAGKDRLSLALIDVRNRSLAWAAALE
                     AAGSPPGREPGRSDGANSDDAGVAAVVAEAPLWRLGHVAWFQEYWIGRNVQRGRGERA
                     DPDLPRLASILPGADDWFASPSIHRGDDQADPVDRATIRQYLLDTLEASLDTLAATPD
                     ETDDALYFHRLVLMHEEMQVEAFAVFAQSFGLRPPEGLLERATSSTVRPPLQVPATRW
                     LLGGGGEGFRFDNEREAHAVEIPEFEIDAQPIRWTQYGEFVEDGGYDEPSFWSPAGRE
                     WLRQGRRTPRHVEQMRRGVVQRRFGLMTRLPNDAPVMHVNCHEAEAWCRWAGRRLPGE
                     AEWEAAAHQAASRGFRFGEVREWTATTFRPYPGFVAGPWRGYSVPSFGVARVLRGASF
                     ATPPALRSTRFRGFASPDCDEGFFGFRSCAA"
     gene            6658..7137
                     /locus_tag="VFF72_11155"
     CDS             6658..7137
                     /locus_tag="VFF72_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZV93767.1"
                     /translation="MDVSLSLTVNGKAVRATIDPRTLLVQFLRENLQLTGTHVGCDTA
                     QCGACTVHMNGRAIKACSMLALQVQGSDITTIEGLAKGDELHPMQAAFRECHGLQCGF
                     CTPGMVMSAVQLLADNPKADEAAIREALDGNICRCTGYQNIVKSIQFCQSGAKPVAA"
     gene            7157..>9035
                     /locus_tag="VFF72_11160"
     CDS             7157..>9035
                     /locus_tag="VFF72_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZV93768.1"
                     /translation="MNEFQVGTIGQSVRRREDRRFLTGDGQYTDDVTTHGQTHGVFLR
                     SPHAHAKIRGIKLDAAKKSPGVIAIFTGADLAEAKVGGLPCGWLIHSKDGAPMKEPPH
                     PVLAQGKVRHVGDQVALVVAETQLQAKDAAEKIEVDYEELPAVIDLAGADSAGATVHD
                     DVANNVCYDWGHGDKAAVDAAFAKAAHVTKLDFVNNRLIPNAMEPRAANASYNKSDQS
                     YTLYVANQNPHVERLLMCAFVLGLPESKVRVIAPDVGGGFGSKIFLYPEDVALVWASK
                     HVGRPIKWTAERSESFLADAHGRDHVTTAEMAMDKDGNFLALRVKTIANMGAYLSTFA
                     SCVPTILYATLLAGQYKTPAIYAEVRAMFTNTAPVDAYRGAGRPEATYVVERIVEQCA
                     RDMKMDPGEIRRKNFVTTFPYATPVGLTYDVGNYAAHLDKAIQMADVAGFKARKAASE
                     ARGRKRGLGYSCYIEACGIAPSNIAGALGARAGLFEAGEVRVHPTGTVTVFTGSHSHG
                     QGHETTFAQVVAGRLGIPVENVEIVHGDTGRIPFGMGTYGSRSLSVGGTAIVKAVDKV
                     IAKGKKIAAHLLEAADTDIEFENGEFKVAGTDKKVPWGSVSLTAYVPHNYPLDKLEPG
                     LN"
BASE COUNT         1428 a         3132 c         3128 g         1347 t
ORIGIN      
        1 cttcagcgcc agcgcgccga tctgcaggat catcaccgcc ttcggcctgg agacggcgat
       61 gttgaagccc cggtaggcgg tgaagacgag cgccggcggc agcgcgaagg cgagcccttg
      121 cagatacgcg cgcaccttct cagcgacgac gccgcgcgcc tgcgccaggt cgagaaacgg
      181 ctcgggcagc aggagaagcg cgcagccgat cagcgacagg gccaggccca gccacatcgc
      241 ctgctgcatc tcgcggccgc tctcgtcgag ccggccggcg ccatagagct ggccggcgat
      301 cggtccgacc gccaggacga tgcccatgaa gccgacgaag atcgagatgt acgccgcggc
      361 gccgatcgcc agcgccgcca ggtcgtcggc cgaggcgcgc gccgccatca ccgtgtcgac
      421 ggtgctgaag gcgatcacgg cgatctggcc gacgaagacc ggccaggcga gcggaaagag
      481 ccggcgcgcg ccgtcgacga gcccgcggcc cctcatgcgg cggcggcgtc gccgtcgtgc
      541 acggctttcg gatcggcgcc cgtgccgcct tcacgggcac ggtcggcgta gcgattgaac
      601 cgccaggcgg tgttctcgtt ggtgatgcga cgccaggtcg cgcgcttctc gcccggcgac
      661 atttccgtcc agtgccggac ttcctcgaag gtgcggccgc agcccttgca gatcgagtcg
      721 ccctggctgg tggagcagat ggcgatgcag ggcgcgtcgg gcatcgtcgc gtaccagtcg
      781 agccaggccg cgtgcgccgc cgccggcatc gacgtttcgt cgcactcgct ctcgtggtag
      841 tagaccatca gcgcgtagac ctcggccagc gccaatacct cgggacaggc cgtgatgccg
      901 tcaggcgagg gatagcgatc gcgccaccag ttgatggccg attcgatgtc gatgatgtgc
      961 agcgccgcca cgtcccaagc tcgcagaagg agccatgacg gtagcactgc cgagggccgc
     1021 cggcgcggac cgcggcgcac gacctcaggg cgtcgcggga tgcttggcga tgacgttgag
     1081 caggccctgg tacttgggtg ccgctgcggc gtcctcggtg acgtactcga gcgcacccca
     1141 cagcccccat ttcgaccagc cgccggcgtc gttgaactgg ttcatcgtct ggccgccgtt
     1201 ggcgatccag agcgagtagt actcgtcgta gacgtccttc atccgcggat tgcggtgcgc
     1261 cgccgagaac agcgccgtca tcgcgtcctg cttgtcggcc ggaaagtacg acgagctgtc
     1321 gccggcgccg ctctcgtacg ccttcatcgt caggtggtac ttggcagcga gcgcggcgtt
     1381 ggccttgatc cagctggtga tgttgccgcg gatgctgacg agcatctggt cgacgacctg
     1441 gtcgctcgtc atctgcagcg ttgcgtcgac cttggtcgtg tcgctggtgc cggcggcgtt
     1501 gaagtaggga gcgatcgcga gcacgtcggc gttggcggcg gtgtcctgcc aggcgagggc
     1561 gttttccagg aattgcgtcc atgccgcctg cccggcgatg acgcggacga gccgtgtgct
     1621 gtcggccggc ccgtacacct gctggaagat cttgaagatc tgcaccgagc gctgcgcgta
     1681 gaacgccgat ggcgtgcccc agggctgcgg cagtccgagc cgggtcgatt gcgccagcgc
     1741 ccaggtggtc tgcgcaaagc cggtgttcca gacctcgttc gagtactcga tgtgcggatg
     1801 cagcgccggg tcgagccggc cgtgcaggag ggtcgcgaac tggcggacgt aatcgtcgct
     1861 cgcctggtgc ggcacgcaga accaggggtc gacgtgcagc gtgttggcca ggtcgatcat
     1921 cacctcgagc ggcacgccgt tggcgttggc gtaggtgacg tcgccgacgt gcgaacggtc
     1981 ggcccattcg acgaccggcg agccgttggt ggcgccccag tccatgaagc gcagcacgga
     2041 gaacggcgcc accttcttca ggaattcgag gttgaacggt tgcgtcgcgt acgtggattc
     2101 ggtgccgggc cagaggaagc gcacgttgcg caccggatcg gcggcattgg tgcggtcgat
     2161 gccgacccac atctcgccgc tggtgtcgct gacgtcgagg acgatgcgat gcggcgctga
     2221 gctgatgacc ttgacgttgc tgagcggaaa gctgatgtcg ccgtcgccgt cccagaggac
     2281 gacgtactgt ccggcggcgt aatgggtgtt ggcccaggcg acggcgtaga gcgcgtgctg
     2341 gttcggattg agcgccgtcg ggtagccgcc ctgcatcgac gggaaggtgc cgccccaggt
     2401 gccgttgtcg tagcgtgacg accagccgcc gccgttcttc atcaggtcgg tgaacgggaa
     2461 ttcggtggtc cagtaggcga tccccgacag gttcatgccg acatactgcg agcgcgtcgg
     2521 caacggcagc gggccgacga ccgacagcgt cgtcgtcgcg gtgatgccat cgacgctcgc
     2581 cgtcgcgatg gtgaagccga cgcgcagcgg cgtcaccgtc ccgtcgccgg cgacctgggc
     2641 gatcgtcgtg tcggcgacgt tccacgacgc cgtcttgtcg ctcaccgcat ggccctgtgc
     2701 atccatcgcc tgcgcggcga gccgggtcgt cgagccgagc ggcaccgccg tcgtcgccgc
     2761 cagcgagacg ctggccacgg gtgcgttgct gccgctgtct tccggaccgc agccggccag
     2821 gccggtcccg atcacaagga cggagaacgc ggcgcgcggg gcgagcgcga cgagagcggc
     2881 gcgcgcggcg ctggcgacgg cgccggtgaa tcgagacagc atgcggattc gcgagacggg
     2941 cactcgcgtc gaacgaggag ccgcattgca ccgtgcgacg cgtcgcggcg tcaacgcaaa
     3001 gtcgttgcag acggaaacag gacgttcccg ccaaacatgc gtaaagctcc ggcgtgacgc
     3061 gaatcacggc ggcgcttcgg cgggcctgcc ttgccccctc cggcaccggt cgcgcattcc
     3121 tgcgcgcggc gccgggcgca gacctacttc ttcggcgctt ccggcaccac gacgatcacg
     3181 gaccccggcg tcgtcgtcgt ggtctccttg atgtcgccgc cgccggcgac gctgcccgag
     3241 accgtgacgc gctggttcgg cgtcagcggc ccgacgatcc gggcgacgca gtcggcctgg
     3301 tccttgcggg gcagcatgcg gcaccggtcg gtggcattgc gcagcggcgt gtcgtcgctg
     3361 gtgagctggt cgtgacgtcg cgcatcgagc gcggcacccg cttccttcag gcaagtggcc
     3421 ttgtcctcgg tcgtgcgccc gctctcgcag gctgctcggt catgggcgta ttgcgcctgc
     3481 ggcgtcatcg ccgagcctga agagccctgc gccatggccc acgggcaggc gagcgcgccg
     3541 gccagtgcgc cagcaaagac gatcggcgac caacctcctc gggtcgacgg aatggaacgg
     3601 atcttcatga tggatctcca gagctgcgca acgtgtgcgg tgtgccgaca cgatagggtc
     3661 ggccgcggta ccggcgccgt aggacggtgc cgccccgaag cgccccacga tggctcgtgc
     3721 cgacgacagc gggcggacaa tgacctgcga caccggcctc ccggccgccg atttgcctgt
     3781 cgaatgccat gaagaaacca tcggtcgagt cacagctctt cgaggcgctc gcctttgcgg
     3841 cgcacaagca tcgggcacag cggcgaaagg atgtcgaggc cagtccctac atcaaccatc
     3901 cgatcgcgct ggcacggacg ctcgcggtcg aaggcggggt caccgacacg cgcaccctca
     3961 tcgccgcggt gctccacgac acggtcgagg acaccgacac gaccttcgag gagatccggg
     4021 cgatgttcgg cgccaaggtc gccgatgtcg tgcgcgaggt caccgacgac acgaagctcg
     4081 acaaggccga gaggaagcgc ttgcagatcg agcacgcgcc gcacctgagc aggcgcgcca
     4141 cgctggtcaa gctggccgac aagacggcca acctgcgcga tgtcgcgtcg aatccgccgc
     4201 gcggctggcc gctcgagcga aagcgcgcct acttcgactg ggccaaggcg gtcgtcgacg
     4261 gcctgccgcc ggtcaacaag cgcctgcgcg ccgcgttcga tgcggcgttc tcgcagcgtc
     4321 cctgaccctt gccaatgcga cgcccgccaa cggcgcgacg cgagccgccg accgaccacg
     4381 cgcgctacgt gcgatccatc cagacgccca ccgacagggc gacgccgatc gcgtacatga
     4441 gccacgtgct cggcacgacg taggggtaga acatgagcgc gacaccgagc catttgatgc
     4501 gcggccgcga ctgatgccgg ccgatgtaga acgcgaccat gccgatcagc ccgaacagga
     4561 tcacgccgac aatgtaggcc gggctcggca gggtgaaacc gagcgattgg agcaacccca
     4621 gttcatcgtg catcgccgac atcgccatcc ttgcccgtcg tgtcgcggct gcgccgcacg
     4681 cggattctcg ctgctcgagc tgatggtcgt catcgcggtg atcgcgatcc tcgcgctgat
     4741 cgcgttgccc ggcctgcccg acaagttcgt gcgcgaccag atcgtcgagg cggtcaagct
     4801 tgccgatctc gccaaggggc cggtcgctgc ggcgtgggcc agccacgtcg cgctgccggc
     4861 ggacaacgcc gcggccggac tgccggcggc caacctcatc gtcaacaacg agatcagctc
     4921 ggtggcgatc gagtccgggg cgatccaggt caccttcggc aatcacgcca acggcaacat
     4981 ccgcggcaag gtgctgagcc tgcgaccggg ggtcgtcgag tcggcgccga tggtgccggt
     5041 ggcctgggtc tgcggcaatg ccgagccgcc gctcaacatg attgccaagg gcccgaaccg
     5101 caccgacgtg ccgctgcgct tcctgccgct caactgccgc gccagcgcgg cctcggcacc
     5161 gggctgacgc ggccatgccg gacggctcgg gcatcgtcgc tgccggactc gagagcgctc
     5221 gcctgcgctc tgccggcaag gatcggctct cgctcgccct catcgacgtg cgcaatcgat
     5281 cgctggcgtg ggccgctgcg ctcgaggccg ccggttcgcc gcccggccgg gagcccggtc
     5341 gatccgacgg cgcgaacagc gacgatgccg gcgtcgccgc ggttgtcgcc gaagcaccgc
     5401 tctggcggct cggtcacgtc gcctggttcc aggagtactg gatcggtcgc aacgtgcagc
     5461 ggggccgcgg cgagcgtgcc gaccccgacc tgccgcggct cgcctcgatc ctgcccggtg
     5521 ccgacgactg gttcgcgtcg ccgtcgattc atcgtggcga cgaccaggcc gatccggtcg
     5581 accgggcgac catccgccag tatcttctcg acaccctgga ggccagcctc gacacgctcg
     5641 ccgcgacgcc cgacgagacc gacgacgcgc tctacttcca tcgcctcgtc ctgatgcacg
     5701 aggagatgca ggtcgaggcg ttcgccgtct tcgcccagtc gttcgggctg cggccacccg
     5761 agggcctgct ggagcgtgcg acctcgtcca ccgtgcggcc gccgttgcag gtgccggcca
     5821 cccgctggct ccttggcggc ggcggcgaag gctttcgctt cgacaacgag cgcgaggcgc
     5881 acgcggtcga gattccggag ttcgagatcg acgcccagcc gatccgctgg acgcaatacg
     5941 gcgagttcgt cgaggacggc ggctacgatg agccgagctt ctggagcccg gccggccgcg
     6001 agtggctccg gcagggccgg cgcacgccgc gccatgtcga gcagatgcgc cggggcgtcg
     6061 tgcagcgccg gttcggcctc atgacgcgcc tgccgaacga cgcgccggtc atgcatgtga
     6121 actgtcacga ggccgaggcg tggtgccggt gggcggggcg ccgcctgccc ggcgaggcgg
     6181 agtgggaggc ggcggcgcat caggcagcgt cgcgcggctt tcgcttcggc gaggtgcgcg
     6241 agtggacggc caccaccttc cggccctacc cgggctttgt cgccggcccg tggcgcggct
     6301 attcggttcc ttcgttcggc gtcgccaggg tgctgcgcgg cgcctcgttc gcgacgccgc
     6361 cggcgctgcg cagcacgcgc tttcgcggct tcgcctcgcc cgactgcgac gagggcttct
     6421 tcggctttcg cagctgcgcg gcctgacgcg cgcccgaccg ctgctcgacg ctatggtcga
     6481 ctgggcccgt ggcggcattt cgatcgctgc cggcaccgcc ctcgactcca aacggcgcag
     6541 ccttggggac tagggaaatc ccgcgcgtgg ccgggcggcg ccggtcgaac aatgggccga
     6601 gcgacgcagc gcggcatcct tcgcagcgcg acgcttcatc cccaacagga gacctgcgtg
     6661 gacgtttccc tttccctcac cgtcaacggc aaggccgtac gggcgaccat cgacccgcgc
     6721 acgctgctcg tgcagttcct gcgcgagaac ctgcagctca ccggcaccca cgtcggctgc
     6781 gacaccgcgc agtgcggtgc ctgcaccgtg cacatgaacg gacgcgccat caaggcctgc
     6841 tcgatgctcg ccctgcaggt gcaaggcagc gacatcacga cgatcgaagg cctcgccaag
     6901 ggcgacgagc tgcatccgat gcaggcggcc tttcgcgaat gccacggcct gcaatgcggc
     6961 ttctgcacgc ccggcatggt gatgtcggcg gtgcagctgc tcgccgacaa cccgaaggca
     7021 gacgaagcgg cgatccgcga agccctcgac ggcaacatct gccgctgcac gggctaccag
     7081 aacatcgtca agtcgatcca gttctgccag tcgggcgcca agcccgtcgc ggcctgagga
     7141 gcgcgccatg ggcagcatga acgaatttca ggtcggcacg atcggccaga gcgtgcgtcg
     7201 ccgcgaggat cggcgctttc tcaccggcga cggccagtac accgacgacg tgacgacgca
     7261 cgggcagacc cacggcgtct tcctgcgctc gccgcacgcg cacgcgaaga tccggggcat
     7321 caagctcgac gcggcgaaga agtcgccggg cgtcatcgcc atcttcaccg gcgccgacct
     7381 cgccgaggcc aaggtcggcg gcctgccctg cggctggctg atccacagca aggacggcgc
     7441 gccgatgaag gagccgccgc atccggtgct ggcgcaaggc aaggtgcgcc acgtcggcga
     7501 ccaggtcgcc ctcgtcgtcg ccgagacgca actgcaggcc aaggacgcgg cggagaagat
     7561 cgaggtcgac tacgaggagc tgcccgcggt catcgacctc gccggcgccg actcggccgg
     7621 cgccaccgtg cacgacgacg tcgcgaacaa cgtctgctac gactggggcc acggcgacaa
     7681 ggcggcggtc gatgccgcct tcgccaaggc ggcgcacgtg acgaagctcg acttcgtcaa
     7741 caaccgcctc atcccgaacg cgatggagcc gcgcgccgcc aacgccagct acaacaagtc
     7801 ggaccagagc tacacgctct acgtcgccaa tcagaacccg cacgtcgagc ggctgctgat
     7861 gtgcgcgttc gtgctcggcc tgcccgagtc gaaggtgcgg gtgatcgccc ccgacgtcgg
     7921 cggcggcttc ggctcgaaga tcttcctcta tccggaagac gtggcgctgg tctgggcgag
     7981 caagcatgtc ggccgtccga tcaagtggac ggccgagcgc agcgagtcgt tcctcgccga
     8041 cgcgcacggg cgcgaccacg tcaccaccgc cgagatggcg atggacaagg acggcaactt
     8101 cctcgccctg cgcgtcaaga cgatcgccaa catgggcgcc tacctgtcga cgttcgcgtc
     8161 gtgcgtgccg accatcctct acgcgacgct gctcgccggc cagtacaaga cgccggcgat
     8221 ctacgccgag gtcagagcga tgttcacgaa cacggcgccg gtcgacgcct accgcggcgc
     8281 tggacgtccc gaggcgacct acgtcgtcga gcgcatcgtc gagcaatgtg cccgcgacat
     8341 gaagatggac ccgggcgaga tccggcgcaa gaacttcgtc accacctttc cgtacgcgac
     8401 gccggtgggc ctcacctacg acgtcggcaa ctacgcggcg catctcgaca aggcgatcca
     8461 gatggccgat gtcgccggct tcaaggcgcg caaggccgcg tcggaggcca ggggcaggaa
     8521 gcgcggcctc ggctactcgt gctacatcga ggcctgcggc atcgcgccct cgaacatcgc
     8581 cggcgcgctc ggcgcgcgcg cgggcctctt cgaggcgggc gaagtgcgcg tgcatccgac
     8641 cggcaccgtg acggtgttca ccggctcgca cagccacggc cagggccacg agacgacgtt
     8701 cgcgcaggtc gtcgccggac ggctcggcat tccggtcgag aacgtcgaga tcgtgcacgg
     8761 cgacaccggt cgcatcccgt tcggcatggg cacctacggc agccgctcgc tgtcggtggg
     8821 cggcacggcg atcgtcaagg ccgtcgacaa ggtgatcgcc aagggcaaga agatcgccgc
     8881 ccacctgctc gaggcggccg acaccgacat cgagttcgag aacggcgagt tcaaggtcgc
     8941 cggcaccgac aagaaggtgc cgtggggctc ggtctcgctc accgcctacg tgccgcacaa
     9001 ctatccgctc gacaagctcg agcccggcct gaacg
//
LOCUS       DASRWS010000586         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1694679, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000586 DASRWS010000000
VERSION     DASRWS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694679"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFF72_11165"
     CDS             <1..258
                     /locus_tag="VFF72_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZV93769.1"
                     /translation="DAASDTRTTLYQIGTAIPIGTGLILVSYGESQAKTSYSRITDRI
                     GSIGYDYFLSKNTDLYIAASREKTFMLSSGGALAGGIRVRF"
     gene            complement(383..1084)
                     /locus_tag="VFF72_11170"
     CDS             complement(383..1084)
                     /locus_tag="VFF72_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HZV93770.1"
                     /translation="MSERSRPASKGRPAATRPRPSIAAGKTATEKTAALTDAEILELQ
                     DRLDALPAPLEPLDVCAIDGFLCGILVQPRPVPRERWLARVIDAEGRAPSPGADVSRI
                     RELVGRREAQLQRAISARHWFDPWIFAPDDDLDEGGDVDEGARTAVLGWVAGFAAALE
                     AFPGLLDRSGPHLNEPLALLYRHFDPDDLDDADDLLAEIEAIEPVADLADAVEGLVRA
                     TLLLADEAEAIGREG"
     gene            1337..2857
                     /locus_tag="VFF72_11175"
     CDS             1337..2857
                     /locus_tag="VFF72_11175"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZV93771.1"
                     /translation="MGATEAFASTAEIGHFIDGKPVAGTSGRRQAVFNPATGGVARHV
                     ALASAAEVDAAVAAAKAAWPAWAALPPLRRARILQGFLRLLEEHRDTLAAMITSEHGK
                     VFTDAQGEVTRGIDVVEFACGIPQLLKGDYTEQVSTGIDNWTMRQPLGVVAGITPFNF
                     PCMVPMWMFPVAIACGNSFVLKPSERDPSASIFMAELLHQAGLPAGVFSVVQGDKTAV
                     DALLAHPDVKAVSFVGSTPIARHIYETGARHGKRVQALGGAKNHVVVMPDANLEQAVD
                     ALVGAAYGSAGERCMALSVAVLVGDVADRIVPLVAERARTLVVKNGMELDAEMGPLVT
                     REAKARVDGYIAAGVDEGATLVADGRGLKVKGLEGGFFTGGTLFDHVTPSMKIYREEI
                     FGPVLSCVRVPDFESALKLVNEHEFGNGVGCFTSDGNVAREFARRVEVGMVGINVPIP
                     VPMAWHGFGGWKSSMFGDMHAYGEEGVRFYTRQKSVMQRWPASTPKGAEFAMPTSK"
     gene            complement(2995..3405)
                     /locus_tag="VFF72_11180"
     CDS             complement(2995..3405)
                     /locus_tag="VFF72_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZV93772.1"
                     /translation="MNALTRFVVTLVLALMAAGMAFAQDAAVPVQRSSGGVEYVNGGI
                     GDGARNAMSQMSGFDLKLVFSNPAGEYLVADHVAVKGPSGEVFAVDQAGPWLLLKLPP
                     GSYTVSARYAGATQERAVKVGSGMRTMNWRFAAS"
     gene            complement(3508..>3993)
                     /locus_tag="VFF72_11185"
     CDS             complement(3508..>3993)
                     /locus_tag="VFF72_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZV93773.1"
                     /translation="LPHFVGVSIAQNLGVTLTHIPYRGSAATMVDLAGGNVAAAVSPV
                     TEALALHKAGKIRILATTGATRSPFVPDVPTFKELGIDLVVPLWFAVYGPAHVPEATV
                     DLLRKAIDTGFATAEVKSRLAQLGLVPAPLSPAALEELRRKETAMWGPVVKASGFKPS
                     D"
BASE COUNT          618 a         1412 c         1384 g          579 t
ORIGIN      
        1 gacgccgcgt cggacacccg caccacgctc taccagatcg gcaccgccat cccgatcggc
       61 accggcctga tcctcgtctc gtacggcgag tcgcaggcga agacgtcgta ctcgcgcatc
      121 accgaccgga tcggctcgat cggctacgac tacttcctct cgaagaacac cgacctgtac
      181 atcgcggcgt cgcgcgagaa gacattcatg ctgtcgtcgg gcggcgccct ggccggcggc
      241 atccgggtcc gcttctagcc gggcgacggc cgcaccgcaa gcggccggat cgcaggacgg
      301 cgccgttcgt ggggacggcc cgccgacgcg ggaccgcgca tccccgcaag caccggtcga
      361 gcgcgggtcg agcccgagcc cgtcagcctt cccggccgat cgcctcggct tcgtcggcga
      421 gaagaagtgt cgcccgcacc agcccttcga ccgcatcggc caggtcggcg accggctcga
      481 tcgcctcgat ctcggcgaga aggtcgtcgg cgtcgtcgag atcgtcggga tcgaaatggc
      541 gatagagcag cgccagcggc tcgttcaggt gcgggccgct gcgatcgaga aggcccggaa
      601 aggcttcgag cgcggcggcg aaaccggcga cccagcccag caccgcggta cgagcgccct
      661 cgtcgacgtc gccgccttcg tcgaggtcgt cgtccggggc gaagatccag ggatcgaacc
      721 agtgccgggc cgagatggcg cgctgcaatt gcgcctcgcg acgtccgacg agctcgcgaa
      781 tgcgcgagac gtcggcaccc ggcgaagggg cgcgcccctc ggcgtcgatc acgcgagcca
      841 gccagcgctc gcgcggcacc ggccggggct gcacgaggat gccgcacaga aagccgtcga
      901 tcgcgcagac gtcgagcggc tcgagcggtg cgggcaaggc gtcgaggcga tcctgcagtt
      961 cgaggatctc ggcgtcggtg agcgccgccg tcttctcggt cgcggtcttg ccggccgcga
     1021 tcgacggtcg cggccgggtc gcggccggcc ggcccttcga cgccgggcga gaacgttcgg
     1081 acatcgggcg attgtggcca atgcgacgcc aggcgcggat cgatggatcg tcgatactgt
     1141 ccgcggctgg cgaacgacag ccccgcagtc cctcttcggc gaacggggtt gcccggcgac
     1201 cggtccggcg ctctggaaca atcgttcgct gccccccgcg ccgggcgccg ttcgttcggc
     1261 ccggctcgcg ccgggccttc tggcacagcg cagcccgcgc gcctgtcgcg tccttcacga
     1321 cctgaggaga aacatcatgg gagccaccga ggccttcgcc agcaccgccg aaatcggcca
     1381 cttcatcgac ggcaagccgg tggccggcac gagcggtcgg cggcaagccg tcttcaaccc
     1441 cgcgacgggc ggcgtcgccc gtcatgtcgc actggccagc gccgccgagg tcgacgccgc
     1501 ggtcgctgcg gcgaaggcgg cctggccggc ctgggcggcg ctgccgccgc tgcgccgtgc
     1561 gcgcatcctg caaggcttcc tgcgtctgct cgaagagcac cgcgacacgc tcgcggcgat
     1621 gatcacctcc gagcacggaa aggtcttcac cgacgcgcag ggcgaggtca cgcgcggcat
     1681 cgacgtcgtc gagttcgcct gcggcatccc gcagctcctc aagggcgact acaccgagca
     1741 ggtctcgacc ggcatcgaca actggacgat gcgccagccg ctcggcgtcg tcgccggcat
     1801 cacgccgttc aacttcccgt gcatggtgcc gatgtggatg ttccccgtcg ccatcgcctg
     1861 cggcaacagc ttcgtcctca agccgagcga gcgcgatcct tcggcctcga tcttcatggc
     1921 cgagctgctc caccaggccg gccttccggc cggtgtcttc agcgtcgtgc agggcgacaa
     1981 gacggcggtc gatgcgctgc tcgcgcaccc cgacgtcaag gccgtgagct tcgtcggctc
     2041 gacgccgatt gcgcgccaca tctacgaaac cggcgcccgg cacggcaagc gcgtgcaggc
     2101 cctcggcggc gccaagaacc acgtcgtcgt gatgcccgac gcgaacctcg agcaggcggt
     2161 cgacgcgctg gtcggcgccg cctacggctc ggccggcgag cgctgcatgg cgctctcggt
     2221 cgcggtgctc gtcggcgacg tggccgaccg gatcgtgccc ctcgtcgccg agcgcgcgcg
     2281 cacgctcgtc gtcaagaacg gcatggagct cgacgccgag atggggccgc tcgtcaccag
     2341 ggaagcgaag gcacgcgtcg acggctacat cgccgccggc gtcgacgaag gagcgaccct
     2401 cgtggccgac ggtcgcggcc tcaaggtcaa gggcctcgaa ggcggcttct tcaccggcgg
     2461 cacgctgttc gaccacgtca cgccgtcgat gaagatctat cgcgaggaga ttttcggacc
     2521 ggtcctttca tgcgtccgcg ttcccgactt cgaatcggcg ctcaagctcg tcaacgagca
     2581 cgagttcggc aacggcgtcg gctgcttcac cagcgacggc aatgtcgcgc gtgaattcgc
     2641 gcggcgggtc gaggtgggca tggtcggcat caatgtgccc attcctgtgc cgatggcgtg
     2701 gcacggcttc ggcggctgga agtcgagcat gttcggcgac atgcatgcct acggcgaaga
     2761 aggcgtgcgc ttctacaccc ggcagaaatc ggtgatgcag cgctggcccg cgtcgacgcc
     2821 caagggcgcc gagttcgcga tgccgacgtc gaaatagccg gtcggcgaga cgcgagccct
     2881 cgacgctgcg ggcggaggtg tcctggttgg cctacggagt cgtcccgacg ggagcggacg
     2941 ggtgcaggct tcaggggccg cgggtgacgc gccaggtcga gtcgacctcg gaattcagct
     3001 cgccgcgaag cgccagttca tggtccgcat gcccgagccg accttcacgg cccgctcctg
     3061 cgtcgcgccg gcatatcgcg cgctcaccgt atagctgccc ggaggcagct tgaggagcag
     3121 ccagggcccg gcctgatcga ccgcgaacac ttcgccgctc ggacccttga ccgcgacgtg
     3181 gtcggcgacg aggtattcgc cggcgggatt actgaagacc agcttcaggt cgaatccgga
     3241 catctgcgac atcgcgttgc gggcgccatc gccgataccg ccgttgacgt attcgacgcc
     3301 tccggacgag cgctgcaccg gcacggccgc atcctgggcg aacgccatgc cggccgccat
     3361 gagcgccagg acgagcgtga cgacgaatcg ggtcagggcg ttcatggaca tctccgcaga
     3421 cagcttgcca gggaggatcg gcgcgcggca ggccgggtgc catcggcgag cggcgcatcg
     3481 ccgcgtcgga cggcatccac cgacgtgtca gtccgacggc ttgaaacccg atgccttgac
     3541 gaccggtccc cacatcgccg tctccttgcg gcgcaactcc tccagcgccg ccggcgacag
     3601 cggcgccggc accaggccca gctgggcgag gcgtgacttc acctcggccg tcgcgaatcc
     3661 ggtgtcgatc gccttgcgca acaggtcgac ggtcgcctcg ggaacatgcg ccggcccgta
     3721 gaccgcgaac cagagcggca cgaccaggtc gatgccgagc tccttgaaag tcggcacgtc
     3781 gggcacgaag ggcgaccggg tcgctccggt cgtcgccagg atgcgtatct tgccggcctt
     3841 gtgcaacgcc agcgcctcgg tgaccggcga caccgcggcg gcgacgttgc cgcctgccag
     3901 gtcgaccatc gtcgccgccg agccgcgata cgggatgtgg gtgagcgtga cgccgaggtt
     3961 ttgcgcgatc gacacgccga cgaagtgcgg cag
//
LOCUS       DASRWS010000587         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2247175, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000587 DASRWS010000000
VERSION     DASRWS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247175"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFF72_11190"
     CDS             <1..856
                     /locus_tag="VFF72_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZV93774.1"
                     /translation="GAAVLSAALDASGRPLSLRITSAGDAITPRWMERTVPALAGPID
                     SPDKTTSEGLFDQPYGIAQRIAHVATRSGVPIGYWRSVGHSHNAFFIESFIDELAAAA
                     HEDPLAFRLSLLADAPRHAAVLRLAGERAGWPVEGRAATLPPGHALGVALHESFGSVV
                     AEIVEASIDGDRPRVHRVVCAADVGTVVDPLIVAQQLESAVIFGLGAALYSRIDIEAG
                     VVQQTNFPNYPMLRMAESPVIETYLVPSARAPGGVGEIGTPPLAPALANALFALTGKR
                     LRTLPLVL"
     gene            complement(885..>2743)
                     /locus_tag="VFF72_11195"
     CDS             complement(885..>2743)
                     /locus_tag="VFF72_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93775.1"
                     /translation="AEDEVIGRTSTELGLWSDLRDRDRLRAAIEGAGRIDQMPATIAA
                     RSGRLVSVLVSAARFTMDGRGYMVVNARDVTVAEQTRLEHAAIFGRASIGIALTRDQR
                     FVHANDRFETLFGWTGGGLIGQPGAAVWLDDADYAEIGRLASPLLSSGQPFEIEREMK
                     RRDGSRFWCRLIGQAVDPSRPGMGGTIWIADDITERRRLDAALSAARDAAESASRAKS
                     AFLANTSHEIRTPLNALLGLARLALDEHIEATTRTRYLEQILASAQGLEAILSDILDF
                     SKIEAGKFTVEAAAFDLGQLLEAVQASYRPPAKARGLALDLVVDESLASIVMGDALRI
                     RQILGNFIANAIKFTAEGAIRIDVRAAAPGVVRLAVTDTGTGIAPELQLLLFQPFSQG
                     DVSTTRRYGGTGLGLSICRQLAELMGGRVGVESQPGAGSTFWAELPLPAAMLQGDLLD
                     SAATEGERLQGARVLLVEDNPVNMMIAAATLAQWGVVVSEARDGRMAIDAVEAAARDG
                     RPFDLVLMDVQMPVLSGHDATIELRKRWNAETLPIIALTAAALVSERDMAMAAGMNDF
                     LTKPIDTPKLRRTLARHVRRTPAAPAASTAATLRKAENEAAATSPEASRAAE"
BASE COUNT          362 a          990 c          980 g          411 t
ORIGIN      
        1 gggcgccgcc gtgctgagcg ccgccctcga cgcgtcgggc cggccgctgt cgctgcgcat
       61 caccagcgcc ggcgacgcga tcacgccgcg ctggatggag cggaccgtcc cggcgctcgc
      121 cggcccgatc gacagccccg acaagacgac gagcgagggc ctgttcgacc agccttacgg
      181 cattgcccag cggatcgcgc acgtcgcgac gcgcagcggc gtgccgatcg gctactggcg
      241 ctcggtcggc cactcgcaca acgccttctt catcgaatcc ttcatcgacg agctcgccgc
      301 cgcggcgcac gaagacccgc ttgcctttcg cctgtcgctg ctcgccgacg cgccgcgtca
      361 tgccgcggtc cttcgcctcg ccggcgagcg ggccggctgg ccggtcgagg ggcgagccgc
      421 gacgctgccg ccggggcacg cgctcggcgt ggcgctgcac gaatccttcg gcagcgtcgt
      481 cgccgagatc gtcgaggcgt cgatcgacgg cgaccggccg cgcgtgcatc gcgtcgtctg
      541 cgcagccgat gtcggcacgg tcgtcgatcc gctcatcgtc gcccagcagc tggaaagcgc
      601 cgtcatcttc ggcctcggcg ccgcgctcta cagccgcatc gacatcgagg ccggcgtcgt
      661 gcagcagacg aatttcccga actatccgat gctgcgcatg gccgaatcgc cggtcatcga
      721 gacgtacctc gtgccgagtg cccgggcgcc gggcggcgtc ggtgagatcg gcacgccgcc
      781 gctggcgccg gcgctcgcca acgccctctt cgcgctgacc ggcaagcgcc tgcgcacctt
      841 gcccctcgtt ctctgaaacg gccgcagcgt cggcgcgcgg cgcgtcattc ggctgcgcgc
      901 gacgcttccg gcgacgtcgc ggcggcctcg ttctcggctt tccggagggt cgcggcggtg
      961 gatgcagcag gtgcggcggg tgtgcgccgc acatggcgcg ccagggtccg gcgcaacttc
     1021 ggcgtgtcga tgggcttggt caggaagtcg ttcattcctg cggccatcgc catgtcgcgt
     1081 tcggagacca gggccgccgc ggtcagggcg atgatcggca gcgtctcggc gttccatcgc
     1141 ttgcgcagct cgatggtcgc gtcgtggccg ctcagcacgg gcatctggac gtccatcagg
     1201 acgaggtcga agggccggcc gtcgcgcgct gccgcctcga cggcgtcgat cgccatccgg
     1261 ccatcgcgcg cctcggacac cacgacgccc cactgcgcca gcgtcgccgc cgcgatcatc
     1321 atgttgaccg ggttgtcctc cacgagaagc acgcgagccc cctggaggcg ttcgccttcg
     1381 gtggcggcgc tgtcgagcag gtcgccctgc agcatcgccg caggcagcgg caactcggcc
     1441 cagaaggtac tgccggctcc gggctggctt tcaacgccga cccggccacc catcagctcg
     1501 gcgagctgac ggcagatcga caggcccagg cccgtgccgc cgtagcggcg ggtcgtcgac
     1561 acgtcgcctt gcgagaacgg ctggaacagc agcagctgca actccggcgc gataccggtg
     1621 ccggtgtccg tcacggccag gcgcacgacg cctggcgcgg cggccctgac gtcgatccgg
     1681 atcgcgcctt cggcggtgaa cttgatcgcg ttggcgatga agttgccgag gatctggcgg
     1741 atgcgcaagg catcgcccat gacgatgctg gcgagggact cgtcgacgac gagatcgagg
     1801 gccaggccgc gcgctttcgc gggcggccgg tacgaggcct gcaccgcctc gagcagctgg
     1861 cccaggtcga acgcggccgc ctcgaccgtg aacttgcccg cctcgatctt cgagaaatcc
     1921 aggatgtcgg acaggatcgc ctcgagcccc tgcgcgctgg ccaggatctg ctcgaggtag
     1981 cgcgtccgcg tcgtcgcttc gatgtgctcg tcgagcgcga gacgggccag cccgagcagg
     2041 gcgttgagcg gtgtgcggat ttcgtggctg gtgttggcga ggaacgcgct cttggcgcgg
     2101 ctggccgact cggcggcgtc gcgcgcggcc gacagcgccg cgtcgaggcg gcggcgttcg
     2161 gtgatgtcgt cggcgatcca gatcgtgccg cccatgccgg gtcgcgacgg gtcgaccgcc
     2221 tggccgatca ggcggcacca gaagcggctg ccgtcccggc gcttcatctc gcgttcgatc
     2281 tcgaacggct ggccgctcga cagcagcggc gaggcgagcc ggccgatctc ggcgtagtcg
     2341 gcatcgtcga gccagaccgc cgcgccgggc tggccgatca ggccgccgcc ggtccagccg
     2401 aacagcgtct cgaagcggtc gttggcgtgg acgaagcgct ggtcgcgcgt gagggcgatg
     2461 ccgatcgatg cgcggccgaa gatcgcggcg tgctcgaggc gcgtctgctc ggcgacggtc
     2521 acgtcgcgcg cgttgacgac catgtagccg cggccgtcca tcgtgaaacg cgccgccgaa
     2581 accagcaccg acaccagccg gcccgagcgg gccgcgatcg tcgccggcat ctggtcgatg
     2641 cgcccggcgc cctcgatcgc cgcgcgcagc cggtcgcgat cgcgcaggtc gctccacagg
     2701 ccgagctcgg tcgacgtccg gccgatcact tcgtcctcgg cgt
//
LOCUS       DASRWS010000588         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1384012, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000588 DASRWS010000000
VERSION     DASRWS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384012"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VFF72_11200"
     CDS             complement(<1..1233)
                     /locus_tag="VFF72_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZV93776.1"
                     /translation="MSKRPNIVFIVADDLGFADLGCYGGRVPVSPVLDGLAAHGIRFT
                     QGYANSPVCSPTRFALMTARFQYRLRGAAEEPINSKSRGSTTLGLPPAQPTLPSLLKG
                     AGYRTALIGKWHLGYPPAFGPLRSGYDEFFGPMSGGVDYFTHCDSRGTHDLWQGEAEH
                     AEPGYLTDLISRRTVESIDRFAAPGQPPFFLSVHYTAPHWPWETRDDEALAQEVKDNL
                     FHLHGGSIHTYRRMIAHMDEGIGWIMAALERHGATRDTLVVFTSDNGGERFSDSWPLV
                     GGKMDLMEGGIRVPYVVRWPARTPAGTTTDQLAITMDWVPTFFEAAGTEAHPDYPSDG
                     LSLLKQLADPGAVTDRPLFWRMKHHEQRAMRSGCWKYLKVDEHEYLFDVVTDERERAN
                     LAKRDPARLAGMRAAWQAW"
     gene            complement(1252..2562)
                     /locus_tag="VFF72_11205"
     CDS             complement(1252..2562)
                     /locus_tag="VFF72_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZV93777.1"
                     /translation="MSIDPLPLDWHAARIAERRRAFLSPVLDTFVAYESLPVWRRGEG
                     AYLWDASGRRHLDAMAQNVCISVGYRHPGVDAAVRSQLDEIQHVTTAYAHPQPGHLAE
                     ELVARLPAGHDWVVHFVNSGAEAIDLAVLAARVHTGHFEVLTLRDAYHGMHFTAMAAT
                     GIGHAHQPVPPAPGFVSVIAPHSYHGPFGGDAAPYLDELSRTIAAATSGDVAAMIVEP
                     IQGYGGIVEMPQGYLAGAAQRAREAGGLFIVDEVQTGFGRTGSHFWGFEAHGVVPDAI
                     VMSKGIGNGFPIAAMVTRREIAESMTRRMFFNTYGANPVSCAAARAVLRAIDDEALRA
                     NADRCGKAFERAFAALGERHASIGDVRGRGLLRGVELVGDRRARTPDAAAAARAQSLL
                     REEGVIVGLCGAGHNVLKINPPLCVTEDDIANLAAACDAALCRL"
     gene            2739..>3212
                     /locus_tag="VFF72_11210"
     CDS             2739..>3212
                     /locus_tag="VFF72_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZV93778.1"
                     /translation="MKDGHGSRARRHPEGGHIICPLVTAVRRRHPPSPGEKAMNVRRF
                     VIAGAFAAGALTLAGCGMMGSSSSMAKPMMAPGDMSATLTAASEVPPKASSGTGTAKV
                     NLTGHTLTWTVTYSGLTGPVTAGHFHGPAEPGSNAGVVVPFAGNLSSPITGSATLT"
BASE COUNT          504 a         1110 c         1138 g          460 t
ORIGIN      
        1 ccacgcctgc caggcggcgc gcatcccggc caggcgtgcc ggatcgcgct tggcgagatt
       61 ggcgcgctcg cgctcgtcgg tcacgacgtc gaacagatac tcgtgctcgt cgaccttgag
      121 atacttccag catcccgagc gcatcgcacg ctgctcatgg tgcttcatgc gccagaacag
      181 cgggcggtcg gtgacggcgc cgggatcggc gagttgcttg agcagagaca gaccgtcgct
      241 cggatagtca ggatgggcct cggtgcccgc ggcctcgaag aaggtcggca cccagtccat
      301 ggtgatggcg agctggtcgg tcgtcgtgcc ggccggcgtg cgtgccggcc agcgcaccac
      361 gtagggcacg cggatgccgc cttccatcag gtccatcttg ccgccgacca gcggccagct
      421 gtcggagaag cgctcgccgc cgttgtcgct ggtgaagacg acgagcgtgt cgcgcgtcgc
      481 gccgtgcctt tcgagggcgg ccatgatcca gccgatgccc tcgtccatgt gcgcgatcat
      541 gcgccgatag gtgtggatgc tgccgccgtg caggtggaag aggttgtcct tcacctcctg
      601 cgccagcgcc tcgtcgtcgc gcgtctccca gggccagtgc ggcgccgtgt agtgcacgct
      661 gagaaagaac ggcggctggc ccggcgccgc gaagcggtcg atcgactcga cggtacggcg
      721 cgagatcagg tcggtgagat agcccggctc ggcatgctcc gcctcgccct gccagaggtc
      781 gtgggtgccg cgcgaatcgc aatgggtgaa gtagtcgacg ccgcccgaca tcgggccgaa
      841 gaattcgtcg tagcccgagc gcagcggccc gaaggccggc ggatagccga gatgccactt
      901 gccgatcagc gcggtccggt agccggcgcc cttcagcagc gaaggcagcg tcggctgtgc
      961 cggcggcagg ccgagcgtcg tgctgccgcg actcttgctg ttgatcggtt cctcggcggc
     1021 accgcgcagc cggtactgga agcgcgccgt catcagggcg aagcgcgtcg gcgagcagac
     1081 cggcgaattg gcgtagccct gcgtgaagcg gatgccgtgg gcggcgaggc cgtcgagcac
     1141 cggcgagacg ggaacccggc cgccatagca gccgaggtcg gcgaagccga gatcgtcggc
     1201 gacgatgaag acgatgttcg gacgtttgct catgggagat ctccgtggcc gctacaggcg
     1261 gcaaagcgcg gcatcgcatg ccgcggcgag attggcgatg tcgtcctcgg tgacgcacag
     1321 cggcggattg atcttgagga cgttgtggcc ggcgccgcac aggccgacga tgacgccctc
     1381 ttcgcgaagc agcgactgcg cccgcgcggc cgcggcggcg tcgggcgtgc gggcgcgccg
     1441 gtcgcccacc agctcgacgc cgcgaagaag cccccggccg cgcacgtcgc cgatcgacgc
     1501 gtggcgctcg ccgagggcag cgaaggcgcg ctcgaacgcc ttgccgcagc gatctgcatt
     1561 ggcgcgcaac gcctcgtcgt cgatggcgcg caggaccgca cgcgccgccg cgcacgacac
     1621 cggattggca ccataggtgt tgaagaacat gcgacgcgtc atcgactcgg cgatctcgcg
     1681 acgcgtcacc atggccgcga tcggaaagcc gttgccgatg cccttcgaca tgacgatggc
     1741 atcgggcacc acgccgtgcg cctcgaatcc ccagaagtgc gagccggtgc ggccgaagcc
     1801 ggtctgcacc tcgtcgacga tgaacagccc ccctgcctcg cgggcccgtt gcgccgcccc
     1861 cgcgaggtag ccctggggca tctcgacgat gcctccatag ccctggatcg gctcgacgat
     1921 catcgccgcg acgtcgcccg atgtcgcagc ggcgatcgtt cgcgacagct cgtcgaggta
     1981 gggcgccgcg tcgccgccga acggcccgtg ataggagtgc ggagcgatca cggagacgaa
     2041 cccgggcgcc ggcggcaccg gctggtgcgc gtggccaatg cccgtggccg ccatcgccgt
     2101 gaagtgcatg ccgtgatagg cgtcgcgcaa ggtcagcacc tcgaaatggc cggtatggac
     2161 gcgcgccgcg agcaccgcca gatcgatcgc ttcggcgccg ctgttgacga agtggacgac
     2221 ccagtcgtgg ccggccggca accgcgccac cagctcttcg gcgaggtgcc cgggctgcgg
     2281 atgggcatag gccgtggtca cgtgctggat ctcgtcgagc tgcgaccgga ccgcggcatc
     2341 gacgccggga tgccggtagc cgacgctgat gcacacgttc tgcgccatcg cgtcgagatg
     2401 acgccggccc gaggcgtccc acagataggc gccctcgcca cgccgccaga ccggcaacga
     2461 ctcgtacgcg acgaacgtgt cgagaacggg cgagaggaag gcgcggcgtc gctcggcgat
     2521 gcgggcggca tgccagtcga gcggcaacgg gtcgatgctc atggcgaatt ggacgggaag
     2581 ggacgaaaag gcgcgaccgg aggccgcatc atgccgcgcc ggcgccctcc actcgccgtg
     2641 caccgccgcg accctcgccg gcgcgtgccc gcgccacgcg gtcgcttcgg tctctccctc
     2701 tcctcgccgg ccggccgccg aaccgcttcc ttcaaccggt gaaggacggc cacgggagcc
     2761 gggcacgacg ccatccggaa ggcgggcata tcatctgccc gctcgtgacg gccgtgcgcc
     2821 gccggcatcc accttcccca ggagaaaaag caatgaacgt tcgtcgattc gtgattgccg
     2881 gcgcgttcgc ggccggcgcc ttgacccttg ccgggtgcgg catgatgggt tcgtcctcgt
     2941 cgatggccaa gccgatgatg gcgccgggag acatgtcggc gaccctcacc gccgccagcg
     3001 aggtgccgcc gaaggcgagc agcggaaccg gcaccgcgaa ggtcaacctg accggccaca
     3061 cgctgacgtg gacggtgacg tattccggct tgacgggccc ggtcacggcc ggccatttcc
     3121 acggccccgc agaacccggc tcgaacgccg gcgtggtcgt gcctttcgcc ggcaacctgt
     3181 cgagccccat caccggcagc gcgacgctca cc
//
LOCUS       DASRWS010000589         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2513027, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000589 DASRWS010000000
VERSION     DASRWS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513027"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFF72_11215"
     CDS             <1..517
                     /locus_tag="VFF72_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZV93779.1"
                     /translation="SRPVPSTRVEPRALELRASEATSSAASTPRGTVAAAAPSPPPAA
                     PRDADDDISWGWPAAGPVAVPFDESKNKGLVFRGKAGDPVLAAADGRVVYAGSGLRGY
                     GNLIILKHNATYLTAYAHNQTLLVKDDQTVRKGQKIAEMGSTDADSVQLHFEIRRQGK
                     PVDPAKLLPPR"
     gene            532..1317
                     /locus_tag="VFF72_11220"
                     /pseudo
     CDS             532..1317
                     /locus_tag="VFF72_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(1407..>2097)
                     /locus_tag="VFF72_11225"
     CDS             complement(1407..>2097)
                     /locus_tag="VFF72_11225"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZV93780.1"
                     /translation="VDDGRKLGFHSWHLVPKTALLDPELTLGLPATLTAATGMDAIAH
                     CMETFMAPAVNPPADGIGLDGLERAWKHIERATLDGADREARWNMMSASMQGAMAFQK
                     GLGCVHSLSHSLGGVDPRLHHGTLNAVFLPAVVRFNASADSMRKDRRLERMAHAMGLA
                     ASDERGDAVADAIRSMNARLKLPRGLADLGVDATMFDRIIDGAMADHCHKTNPRLATR
                     DDYVRMIEASM"
BASE COUNT          338 a          734 c          688 g          337 t
ORIGIN      
        1 cagccggccg gtgccctcga ctcgcgtcga gccgcgagca ctcgagctcc gcgcaagcga
       61 agcgacgtcg agcgccgcat cgacgcccag gggcaccgtg gccgccgcgg ctccgtctcc
      121 gccgccggca gctccgcgcg acgccgacga cgacatctcg tggggctggc cggcggcggg
      181 ccccgtggcc gtgcctttcg acgagtcgaa gaacaagggc ctcgtctttc gcggcaaggc
      241 gggcgatccg gtcctcgctg ccgccgatgg ccgcgtcgtc tacgccggct cgggcctgcg
      301 cggctacggc aacctcatca tcctcaagca caacgcgacc tacctgacgg cgtacgcgca
      361 caaccagacg cttctcgtca aggacgacca gaccgtgcgc aagggacaga agatcgccga
      421 gatgggctcc accgacgccg acagcgtcca gcttcacttc gagatccgcc gacagggcaa
      481 gccggtcgat cccgccaagc ttctgccgcc tcgctgaacg tcgagcaccc gatgtcgtcc
      541 tggcccgtcc tcgtcttcga catcgagtcc atccccgacg tcgccgggct gcgcgcgctg
      601 cgccaggccg acgcgagcct cgacgacgcc gccgtgtacg ccgccgaaat ggccgatcgc
      661 caggcgcgcg gcaagagcga cttcatgccg ctgcacttgc agcgcgtcct cgtcatcagc
      721 tgcatctttc gaaatgccga tggcttgcgc gtgcactcca tcgtcgaccg cgaaaccgcc
      781 ggcaagagcc aggaaggccg ggtcatccag tccttcttca acctcgtcga acgccacgtg
      841 ccccagctcg tcagctggaa cggaggcggc ttcgacctgc cggtgctgca ttaccgcggc
      901 cttcgtcacg gcgtcgtcgc cagcaagtac tgggacatgg gcgaggacga tcgcgagttc
      961 cgcttcaacc actacatcgg ccgctatcac cttcgccacc tcgacctgat ggacctgctg
     1021 gcgatgtacc agcctcgtgc caatgcgccg ctcgatgcga tggccaggct gtgcggcttt
     1081 ccgggcaagc tcggcatgga cggctcgcag gtgtactcgg cctatctcga aggaaggctc
     1141 gaggagatcc ggcgctattg cgagaccgac gtgatgaaca cgtatctcct ctactgccga
     1201 ttccagaaga tgcgaggcgg cttcaccgag cccgaatacg atcgcgaaat ggcgctggtc
     1261 ggcgagacgc tgcaaggaat cgacgagccg cactggcgag aatatctcgc tgcgtggcgg
     1321 gatcgaccag aagcatcgcg aggctcggcg atggggaccg atctgccccc cggggatccg
     1381 ctcgcgacgc agtcgggcgc ggccgtctac atcgacgctt cgatcatccg gacgtagtcg
     1441 tcgcgcgtcg ccaggcgcgg attcgtcttg tggcagtggt cggccatcgc gccgtcgatg
     1501 atccggtcga acatcgtcgc atcgacgccg agatcggcga ggccgcgcgg cagcttcagg
     1561 cgggcgttca tcgaccggat cgcgtcggcg acggcgtcgc cgcgttcgtc gctcgccgcc
     1621 aggcccatgg catgcgccat ccgctccaga cgccggtcct tgcgcatcga atcggcggac
     1681 gcattgaacc tgaccaccgc aggcaggaag acggcgttca gcgtgccgtg gtgcaggcgc
     1741 ggatcgacgc cgccgaggct gtggctcagg ctgtggacac agcccagtcc cttctggaat
     1801 gccatggcgc cctgcatgct cgcgctcatc atgttccagc gggcttcccg gtcggcgccg
     1861 tcgagagtgg cccgctcgat gtgcttccat gcccgctcca ggccgtcgag cccgatgccg
     1921 tcggcgggcg ggttgacggc cggcgccatg aaggtttcca tgcaatgggc gatcgcatcc
     1981 atcccggtcg ccgcggtgag ggtggccggc aggccgagcg tcagttccgg atcgaggagg
     2041 gcggtcttcg gcacgaggtg ccagctgtga aagccgagct tgcgaccgtc gtcgacg
//
LOCUS       DASRWS010000590         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1944094, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000590 DASRWS010000000
VERSION     DASRWS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944094"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFF72_11230"
     CDS             complement(<1..578)
                     /locus_tag="VFF72_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZV93781.1"
                     /translation="MHRLLGRRLIAPAAQHVLRHPGRFAWQTLKAFRANQGLLLAGAV
                     AYYALLSIVPLLILTVIVLSHLIDQRELLGTIGRYLEWLVPGQSSAIVGELAHFLDNR
                     GDVGWLLLATMVFFSSLAFTVLENAMSVIFIHRVVARKRRFIFSVLIPYCYILSLGVG
                     LLLVTLVAGSLQAVGQESVDLFGHVWSLHGLSG"
     gene            complement(593..1012)
                     /locus_tag="VFF72_11235"
     CDS             complement(593..1012)
                     /locus_tag="VFF72_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZV93782.1"
                     /translation="MTRVSEIMTRGIAVVQRDETLQAAARRMREMDVGALPVADGKAL
                     AGMVTDRDIAVRGVAEGMIPQESLVSDVMTADVFWCRDSDSVEDAMAQMGRSQVRRLA
                     VLDANDEIVGIVALGDLATRQSSHTDEALREISTPGH"
     gene            complement(1260..2387)
                     /gene="tgt"
                     /locus_tag="VFF72_11240"
     CDS             complement(1260..2387)
                     /gene="tgt"
                     /locus_tag="VFF72_11240"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZV93783.1"
                     /translation="MLSFDVVAREGLARRGRLKLNHGSVETPVFMPVGTYGSVKGVMP
                     STLEAMGAHIILGNTFHLWLRPGPDVLKRFGGLHRFEGWPRPILTDSGGFQVWSLGAN
                     AKVSEQGVAFASPVNGDKLLLTPEVSMQIQRTLDSDIAMQFDECTAYQVEGRVAGEDA
                     VRRSMQMSLRWAARSRDEFVRLENPNALFGIVQGGMFESLREESVDALAALDLPGYAI
                     GGVSVGEPKQEMQRIVAHTPQRLPEHKPRYLMGVGTPEDLLDGVAAGVDMFDCVMPTR
                     NARNGHLFTRFGDLRIRNARHKEDESPIDPTCGCATCARFSRAYLHHLDRCGEMLGPM
                     LASQHNLFFYLDLMRQVRESLEVGGFAEFALAWRSDRRRGV"
     gene            complement(2417..>2842)
                     /locus_tag="VFF72_11245"
     CDS             complement(2417..>2842)
                     /locus_tag="VFF72_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HZV93784.1"
                     /translation="LHVGAGTFQPVRSEDIAGHVMHRERYAVDAVAVAAIERARSRGG
                     RIVAAGTTSLRALEAAALAGEPLSPQQGDTDLFITPGFRFRVVDLLLTNFHLPRSTLL
                     MLVSAFAGHDRIMALYRHAVAERYRFFSYGDAMLLAHAS"
BASE COUNT          508 a          931 c          993 g          410 t
ORIGIN      
        1 cccgacaggc catgcaacga ccagacatgg ccgaacagat cgacgctctc ctggccgacc
       61 gcctgcaggc tgccggcgac gagcgtcacc aggagcaggc cgaccccgag cgacaggatg
      121 tagcaatagg gaatcaggac cgagaagatg aagcggcgct tcctggcgac gacgcggtgg
      181 atgaagatca ccgacatcgc gttctcgagc acggtgaacg cgagtgagct gaagaagacc
      241 atcgtcgcca gaagcagcca gccgacgtcg ccgcggttgt cgaggaagtg ggccagctcg
      301 ccgacgatcg ccgacgactg tccgggaacc agccattcga gatagcggcc gatcgtgccc
      361 agcagctcgc gctggtcgat caggtgcgaa agaacgatca ccgtcaggat cagcagcggc
      421 acgatcgaca gcagcgcgta gtaggccacc gcgccggcca gcaaaaggcc ctggttggcg
      481 cgaaacgcct tcagcgtctg ccaggcgaag cggccgggat ggcggagaac gtgttgcgcc
      541 gccggcgcga tcagcctgcg gccgagaagg cgatgcagtg cgttggacga attcaatgac
      601 ccggcgtcga aatctcgcgc agcgcctcgt cggtgtgcga cgactgccgc gtcgccagat
      661 cgccgagggc gacgatgccg acgatctcgt cgttggcgtc gagcacggcg aggcggcgta
      721 cctgggatcg gcccatctgc gccatcgcgt cctcgacgct gtcgctgtcg cggcaccaga
      781 agacatcggc ggtcatcaca tcggaaacga gcgactcctg cgggatcatg ccttcggcga
      841 cgccgcgcac ggcgatgtcg cgatcggtga ccatgccagc gagggccttg ccgtcggcga
      901 ccggcaacgc gccgacgtcc atctcgcgca tgcgccgggc agcggcctgc agcgtctcgt
      961 cgcgttgaac gacggcgatg ccgcgcgtca tgatttcgga aactcgggtc atggggtgag
     1021 cgcgaaagga gtgcgatcgg gacgacgatc ggcaacggga tgccgagccg aagcccggag
     1081 tatggatctc gtctcgggat ctcccggggc gaagccttcg agcgggcgct caccgaggaa
     1141 gcgaacgacc agagcaggtt attccggcaa cgtgccagtg gccaccggat cggcgacgcg
     1201 acaccgtgtc ggtcgccagc ccccgatgcg gtcggcaacg tccgacgccg gaccgacgcc
     1261 tagacgccgc ggcggcgatc gctgcgccag gccagggcga actcggcgaa accgccgacc
     1321 tccagcgact cgcgcacctg gcgcatcagg tcgagataga agaagaggtt gtgctggctc
     1381 gccagcatcg gccccagcat ttcgccgcaa cggtcgaggt ggtgcagata ggcgcgcgaa
     1441 aagcgcgcgc aggtggcgca gccgcacgtc gggtcgatcg gcgactcgtc ctccttgtgg
     1501 cgggcattgc ggatgcgcag gtcgccgaac cgggtgaaga gatggccgtt tctggcgttg
     1561 cgggtcggca tgacgcaatc gaacatgtcg acgccggcgg cgacgccgtc gagcaggtcc
     1621 tccggcgtgc cgacacccat gaggtagcgc ggcttgtgtt ccggcagccg ctgcggcgta
     1681 tgggcgacga tgcgctgcat ctcttgcttc ggctcgccga cgctgacgcc gccgatcgcg
     1741 tagccgggca ggtcgagcgc ggccagcgcg tcgaccgact cctcgcgcaa ggactcgaac
     1801 atgcctccct ggacgatgcc gaacaacgcg ttcgggtttt ccagccgcac gaactcgtcg
     1861 cgcgagcgag cggcccagcg caggctcatc tgcatcgaac ggcgcaccgc gtcttcgccg
     1921 gcgacgcggc cttcgacctg gtacgcagtg cactcgtcga actgcatcgc gatgtcgctg
     1981 tcgagcgtgc gctggatctg catgctgact tccggcgtca gcagcagctt gtcgccgttc
     2041 accggcgagg cgaaagccac gccttgctcg ctcaccttgg cgttggcacc gagcgaccag
     2101 acctggaagc cgccgctgtc ggtgaggatg ggacgcggcc atccctcgaa gcgatgcagg
     2161 ccgccgaagc gcttcaagac gtcgggtccg gggcgcaacc agagatggaa ggtgttgccc
     2221 aggatgatgt gcgcccccat cgcctcgagc gtcgacggca tgacgccctt gacgctgccg
     2281 taggtgccga ccggcatgaa gaccggcgtc tcgacgctgc catggttgag tttcagccgg
     2341 ccgcgccggg ccaggccctc gcgagcgacg acatcgaagg acaacattgc cgtgattgtc
     2401 gcggtgcgcc gcggcctcag cttgcatggg cgagcagcat cgcatcgccg tagctgaaga
     2461 atcgatagcg ctcggcgacg gcatgccggt acagcgccat gatccgatcg tgcccggcga
     2521 atgccgagac cagcatcaac agcgtgctcc tgggcaggtg gaagttggta agcaggaggt
     2581 cgacgacgcg aaaccggaat cccggcgtga tgaagagatc ggtgtcgccc tgctgcggcg
     2641 acagcggctc gccggccagg gccgcggcct cgagcgcgcg caggctggtc gttccggcgg
     2701 cgacgatgcg tccgccgcgc gagcgggccc gctcgatcgc cgcgacggcc acggcgtcga
     2761 cggcatagcg ctcgcgatgc atcacgtgcc cggcgatgtc ctcgctgcgt accggctgga
     2821 acgtgccggc gccgacatgc aa
//
LOCUS       DASRWS010000591         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_816475, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000591 DASRWS010000000
VERSION     DASRWS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816475"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VFF72_11250"
     CDS             complement(<1..1028)
                     /locus_tag="VFF72_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HZV93785.1"
                     /translation="MKVKISRALIVAAALTVMAATMTMAQEPEALALTGDFWGTHDPS
                     VIKAGSTWYVFATGKAQEGGQFQIRCSPDLHAWKLCGHVFDQIPEWIQKESPGTKELW
                     APDISFEHGEYRLYYAWSLFGKNTSGIALATNKTLDRSSPDYRWVDRGAVLRSTAADD
                     YNAIDPNFVLDEQGHSWLAFGSFWSGIKMRRLDDKTGLAGNPKLYALATRRRPDNPPP
                     APPGLPADWEAIEAPFIVHHGGYYYLFVSWDLCCRGVHSTYRTMVGRSASVTGPYVDE
                     QGVPMMQGGGTPLLSGNERWLGPGGESLFMQRSGPDIIVFHAYDAHSGKPALQVSTLA
                     WRNGWPHAA"
     gene            complement(1075..>2501)
                     /locus_tag="VFF72_11255"
     CDS             complement(1075..>2501)
                     /locus_tag="VFF72_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZV93786.1"
                     /translation="QWGFDGFVTDDLGAVALLTGSSERNPTEPGQRLTEDPVVAAAMA
                     IKAGNDSDDPEFEKNIPLAVQRGLLTQQDVDRALRRVLRVGFRLGAFDPPENNPYSKL
                     GMDVVRSQAHLDLAEKVAAESITLLSNRRNFLPLRREEIHSVAVIGPAGGDDYETGNY
                     YGKPTRKVSVVSGIKDLLGSGVSVSYEQGAGFTEAYDAQAVQRAVDLARRSDVVILCL
                     GTNLHVEAEGHDRRDLQLPGAQEQLLEAVYAANPRTALVLMTAGPLAATWAQDHLPAI
                     LSAWYPGETGGTAIARALFGLDNPGGHLPYTVYASLDNVPPPNDYAVNDGFTYQYFKG
                     LPLYPFGFGLSYTRFAYSHLHITPAVSANGQADISFELRNTGERAGAEVAQLYTHLEK
                     SRAYQPIRSLRAFQRVELQPGEAQTIHLRMPMKQLAWYDTARRAFLVEPGIYDVQIGS
                     SSDDIRLRGKITVGSASASHHDKQ"
BASE COUNT          441 a          726 c          862 g          472 t
ORIGIN      
        1 gccgcgtgcg gccagccgtt gcgccacgcc agtgtcgaga cctggagggc gggttttccg
       61 gagtgcgcgt cataggcgtg gaagacgatg atgtcgggac cggatcgctg catgaagagc
      121 gattcgccgc ccgggccgag ccatcgttca ttgccgctga gtaagggagt gccgccaccc
      181 tgcatcatcg ggacaccctg ctcgtcgacg tacgggccgg tgacgctggc ggagcggccg
      241 accatggtcc ggtaggtgct gtgcacgccg cgacagcaga ggtcccagga gacgaagaga
      301 taatagtagc cgccatgatg gacgatgaag ggcgcctcga tcgcctccca gtcggcaggc
      361 aggccgggcg gcgccggcgg aggattgtcg ggccggcggc gcgtcgcgag cgcatacagc
      421 tttggattgc cggccagacc ggtcttgtcg tcgaggcggc gcatcttgat gccgctccag
      481 aagctgccga atgcaagcca ggagtggccc tgctcgtcga ggacgaaatt ggggtcgatg
      541 gcgttgtagt cgtctgccgc ggtggagcga agaacggcgc cgcgatcgac ccagcggtag
      601 tcggggctgg agcggtcgag tgttttgttc gtggccagcg caatgcccga ggtgtttttg
      661 ccgaagaggg accaggcgta gtagaggcgg tattctccgt gttcaaagga gatatcggga
      721 gcccaaagct ccttcgttcc ggggctctct ttctgaatcc attcggggat ctggtcgaat
      781 acatggccgc ataacttcca ggcgtggagg tcgggcgaac accgaatctg aaactggccg
      841 ccctcctgcg cctttccagt tgcgaacacg taccaggtgc tgccggcttt gatcacagaa
      901 ggatcatgcg tgccccagaa atccccggtg agggccagcg cttcgggctc ctgtgccatc
      961 gtcatagtcg ccgccatgac ggtgagtgca gccgcgacta tcaatgcacg actgattttt
     1021 actttcatgc ttcgcacgga acagagggtc ctggtcgtca ctgcctgact cccatcattg
     1081 tttgtcgtga tgcgacgcgc tcgcagagcc gacggtgatc tttccgcgaa ggcggatatc
     1141 gtccgacgac gagccgatct gcacatcgta aatgccgggc tcgacgagga atgcacgacg
     1201 tgccgtgtca taccaggcga gctgtttcat cggcatgcgc aagtgtatcg tctgcgcctc
     1261 gcccggctgc agctccactc gctggaaggc ccgcaggctc ctgattggct gatacgcgcg
     1321 gctcttctcc aggtgcgtgt agagctgcgc gacttccgct ccggcgcgtt cgccggtatt
     1381 gcgcagttcg aaagagatgt ccgcctgtcc gttcgcggaa acggcgggag tgatgtggag
     1441 atggctgtac gcgaagcgcg tatagctcag gccgaagcca aacggataca gtgggagtcc
     1501 tttgaagtac tggtaggtga atccatcgtt gacggcgtag tcgttcgggg gaggcacgtt
     1561 gtcgaggctc gcgtaaaccg tgtacggcag atggccgccg ggattatcga gtccgaagag
     1621 agcacgggcg atggcggtgc cgccggtctc gccgggatac cacgcgctca ggatcgcggg
     1681 cagatgatcc tgcgcccagg tggcagccag cggccctgcg gtcatgagga cgagagcggt
     1741 cctgggattg gcagcataga ccgcttcgag aagctgctcc tgtgcgccgg gaagctgcag
     1801 atcgcgccga tcgtgccctt ctgcttcgac atgcaaattc gtgccaaggc acagaatgac
     1861 aacgtcgctg cggcgggcga gatcgacggc gcgctgcacg gcctgggcgt cgtaagcctc
     1921 ggtgaaaccg gctccctgtt cgtacgagac cgaaacgccg ctgcccagca gatccttaat
     1981 gccggagacg acgctgacct tgcgcgtggg cttgccgtag taattgccgg tttcgtagtc
     2041 gtcgccgccg gcgggcccga tgacggcgac ggagtgaatc tcttcgcgcc gaaggggaag
     2101 aaagttcctg cggttgctga gcagcgtgat ggactcagcg gccacttttt cggccaggtc
     2161 gagatgcgcc tgcgagcgca cgacatccat gccgagcttg ctgtacgggt tgttctccgg
     2221 cggatcgaag gcgcccagac ggaagccgac gcggagaacg cgacgcaggg cgcgatcgac
     2281 gtcctgctgc gtgaggagtc cccgctgcac ggcgagggga atgttcttct cgaattcagg
     2341 atcgtcggag tcattgccgg ctttgatcgc catggcggcg gcgaccaccg ggtcttcggt
     2401 gaggcgctga ccgggctcgg tggggttcct ctcggagctg ccggtgagaa gcgccacggc
     2461 tcccagatcg tcggtgacga agccgtcgaa tccccactgg c
//
LOCUS       DASRWS010000592         6441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1109481, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000592 DASRWS010000000
VERSION     DASRWS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109481"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..594)
                     /locus_tag="VFF72_11260"
     CDS             complement(115..594)
                     /locus_tag="VFF72_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010111883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZV93787.1"
                     /translation="MKTSPARSATRKVTKIAPRSATESAAKNATKGATSQGASASALI
                     DARIAELGGWRGDMLSRIRALIRAADPEVVEEWKWRNPVWSHDGLICTGEAYKEVVKT
                     TFAKGASLPDPAALFNSSLDGNVRRAIDFHEGDEIDKKALKDLVRAAVALNEASGRG"
     gene            complement(597..1010)
                     /locus_tag="VFF72_11265"
     CDS             complement(597..1010)
                     /locus_tag="VFF72_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV93788.1"
                     /translation="MTQGIEVAGVYVHDQDEALAFYVDKLGFRVHADVKNGTYRWLTV
                     QHPDQPSFQLGLFAPGPPVHDEATAQTLREVVAKGAMPPLVLVVDDCRAAHDRMRARG
                     VEFTQEPVERYGTVDAGFRDPSGNGWKMIQARPRG"
     gene            complement(1007..1501)
                     /locus_tag="VFF72_11270"
     CDS             complement(1007..1501)
                     /locus_tag="VFF72_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZV93789.1"
                     /translation="MNDRPARQDPALLRRLLRAKDRMDAASHEAWPVERLARVSGVSE
                     AHFARSFRQAFGVPPHRYLLTRRIERATALLRDTGLSVTEIAFDTGWESLGTFGRTFR
                     DVTGESPSEARARARAAAHALGQVPGCIVNAAQRPGLTIAVSEKRRRQGGDTNEPSER
                     QEAR"
     gene            complement(1593..2036)
                     /locus_tag="VFF72_11275"
     CDS             complement(1593..2036)
                     /locus_tag="VFF72_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV93790.1"
                     /translation="MPAPSTHPAPLSPFHLAFPVHDLAAARSFYGDLLGCPEGRSADD
                     WVDFNFHGHQIVAHLAPAETQPGHRNAVDGHDVPVRHFGIVLPMAEWEALAARLRARG
                     TTFVIEPYVRFKGEPGEQATMFFLDPSGNAIEIKAFADIGMLFAK"
     gene            complement(2210..3382)
                     /gene="argE"
                     /locus_tag="VFF72_11280"
     CDS             complement(2210..3382)
                     /gene="argE"
                     /locus_tag="VFF72_11280"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HZV93791.1"
                     /translation="MSAAPSAHALELARSLVRMRTVSRDSNLELIHFIRDELARHGVA
                     STLTFDDERKKANLFATLGEGKPAGVILSGHTDTVPWDGQDWTLDPLGAELRDGRLFG
                     RGSADMKSFIGLAVAQVPAYLSADLPFSVHLAFSYDEEVGAFGVPRLIADLQERGVRP
                     LACIVGEPTGMVPALAHKGVYRWRCCVRGHAAHSSLTPQSVNAIEVGARVVGKLSDMA
                     ARFRDDAPRYEGFDVPYTTGSVGVIEGGIADNIVPADCRFNYEFRNLPGTDAEAMQGE
                     VRAFAASLEPAMRAVAPETGIRFETICAVPAFLAREDDVAVRLARRLVGVGETTLVAF
                     GTEAGLFQKAGIPTVVCGPGHIAQAHQPDEYVSLAQLAACERFLCDLKTLPVDALA"
     gene            3794..4420
                     /locus_tag="VFF72_11285"
     CDS             3794..4420
                     /locus_tag="VFF72_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93792.1"
                     /translation="MDRWTFYTAPGTCALATHIALHEAGAAFDVVTVDFAAHQQQSPA
                     YLAVNPKGRVPALATPAGVLTETPALLLFVAQRFPEARLAPLDDPFALARLNEFDSYL
                     ASTVHVAHAHKRRGSRWADDPAAHEAMRAKVPQSMSACAALLEARLAPGPWVLGERYS
                     VADGYLFTLSGWLEGDGVDMNRFPRLVAHRDRVAQRPAVQRALAGAGS"
     gene            4540..5340
                     /locus_tag="VFF72_11290"
     CDS             4540..5340
                     /locus_tag="VFF72_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93793.1"
                     /translation="MLYRHTMSSRAHRLVDALAHRQYDANEESSDRGPEKTMTRVSVD
                     SLSFTVREVDSPEDMARVCELRAECYGRRAPALREPMAHPDAFDLSPSTTVFLCEDKT
                     SGELVGTMRVVDNTLDGDLDIEEFIEVPGWLSSSRRGEMTRLVVAPGADPLVKAALWK
                     AGYLRCLQARVQWLIIAARKPGLIKQYEYLGAKDLYADQRLVPVGRGWSSPHRIFVFD
                     VLSAKDDWYEGRHRLLRFMVETEHPDIRLASMHGAAAPAPRLSLVASA"
     gene            complement(5284..>6441)
                     /locus_tag="VFF72_11295"
     CDS             complement(5284..>6441)
                     /locus_tag="VFF72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93794.1"
                     /translation="ILGVTAMMKRDHQDPVSLSRLGIVQAQGEHLLGLIGALLDVSRI
                     ETGRLELHLAPFDLAVEVRQLAELYRVRTSGTSVAFVLEGQLPSSCWVLGDAPRVRQV
                     LHNLLGNSVKFTKEGWIRLGVERGKQGETVCTVTDTGPGISVSDQARIFEAFAQTDSV
                     ATQPDAGTGLGLAISRELAHAMGGEVTVESIVGVGSKFQFRALLPAVDAPTQDDDSHG
                     AAANSSAAPRSLVGYRVLLAEDNDVNALIAEAVLKQLGAEVSRVSSGRAAVAAALGSQ
                     RPHAVLMDLRMPDLDGLSATREIRARERAARISRLPIIALTANSSRDDVNACEAAGMD
                     GFLSKPFTDEGLARVLAAHLGPGIPLLADENDDLGGGHETQPWSGRSGAVH"
BASE COUNT         1018 a         2210 c         2187 g         1026 t
ORIGIN      
        1 cgcggcgatg ggtcgccggc ggcctggcat cggcttgcct gtcggctcgg attgcctgtg
       61 gttcttgcgt gtgcgcggcg tcttgcgtcg ggcgccacat cgcggcgggc gcgactagcc
      121 gcggcccgac gcctcgttca acgccacggc cgcccgaacg aggtccttca gcgccttttt
      181 gtcgatctcg tcgccctcgt gaaagtcgat ggcacgcctc acgttgccgt cgaggctgga
      241 gttgaagagg gcggcgggat ccggaagcga cgcacccttc gcgaacgtcg tcttcacgac
      301 ttccttgtag gcctcgccgg tgcagatcag gccatcgtgc gaccagaccg gattgcgcca
      361 tttccattcc tcgaccactt cggggtcggc cgcccggatc agcgcgcgga tccgggagag
      421 catgtcgccg cgccagccgc ccagctccgc gatcctcgcg tcgatcagcg cggaagcgga
      481 cgcgccttgc gacgtcgcac ccttggtggc gttcttcgcg gcgctctcgg tggcgctcct
      541 cggcgcgatc ttcgtcactt tccgggtcgc actccgggcc gggctcgtct tcatcgtcat
      601 cctcgcgggc gagcctggat catcttccag ccattgcccg acgggtcgcg gaagccggcg
      661 tcgacggtgc cgtagcgctc gaccggctcc tgcgtgaact cgacgccgcg cgcgcgcatc
      721 cggtcgtggg cagcgcggca atcgtcgacg acgagcacga gcggcggcat cgcgcccttg
      781 gcgacgacct cgcgcagcgt ctgcgccgtc gcctcgtcgt gcaccggcgg ccccggcgcg
      841 aagaggccga gctggaacga cggctggtcg ggatgctgca ccgtcagcca gcgataggtg
      901 ccgttcttca cgtcggcatg aacgcgaaag ccgagcttgt cgacatagaa cgcgagcgct
      961 tcgtcctggt cgtgcacgta cacgcccgcc acttcgatcc cctgggtcat cgtgcctcct
     1021 gtcgttcgga aggctcgttt gtatcgccgc cttgccggcg ccgcttctcc gaaactgcga
     1081 tcgtcaggcc gggccgctgc gcggcattga cgatgcagcc cggcacctgg ccgagcgcat
     1141 gcgcggcggc acgtgcccgc gcacgcgctt cgctcgggct ctcgccggtg acgtcgcgga
     1201 aggtgcggcc gaacgtgccc aggctctccc agccagtgtc gaaggcgatc tcggtcaccg
     1261 acaacccggt gtcgcgcagg agcgccgtgg cccgctcgat gcgccgggtg agcaggtagc
     1321 ggtgcggcgg aacgccgaac gcctgcctga acgaacgtgc gaaatgcgcc tcggaaacgc
     1381 cgctcacgcg ggcgagccgc tcgacgggcc acgcttcgtg cgaggccgca tccatccggt
     1441 ccttggcacg aagcagtcgc cgcagcagtg cgggatcctg cctcgcggga cgatcattca
     1501 tgacggatcg atgcctgcct cgtgctggcg gcgatcttgc cgtcgaccgc ccgcccgcac
     1561 atcgacgacc gccgaccgcc gcgaccggga catcacttcg cgaacagcat cccgatgtcg
     1621 gcgaacgcct tgatctcgat ggcgttgccc gaggggtcga ggaagaacat cgtcgcctgc
     1681 tcgccgggct cgcccttgaa gcgcacgtag ggctcgatca cgaaggtggt tcccctggcc
     1741 cgcaggcgcg cggcgagcgc ttcccattcg gccatcggca ggacgatgcc gaagtggcgc
     1801 accggcacgt cgtgcccgtc gacggcgttg cgatgcccgg gctgcgtttc ggcgggcgcc
     1861 aggtgggcga cgatctggtg gccgtggaaa ttgaagtcga cccagtcgtc ggcgctgcgc
     1921 ccttcggggc agcccagcaa gtcgccgtag aagctgcgcg cggcagcgag gtcgtgcacc
     1981 ggaaacgcga ggtgaaaagg cgacagcggt gccggatggg tgctgggagc gggcatgttg
     2041 cggatcctgg gataccgtgg ggtaggtgat gcaatggtag agaccgggaa cgcgaagcgc
     2101 gatcggcgat cgacgcgacc gggacaagat cagggccgat cccgcggccg gatgtcgtga
     2161 cggcaccagg caggccggac gcagcgtccg gcccacggca ggcgacgtgt caggcgagtg
     2221 cgtcgacagg cagggtcttc agatcgcaaa gaaatcgctc gcacgccgcg agctgcgcca
     2281 gcgacacgta ctcgtcgggc tgatgcgcct gggcgatgtg tcccgggccg cagacgacgg
     2341 tgggaatgcc cgccttctgg aagaggccgg cctcggtgcc gaaggccacc agcgtcgtct
     2401 cgccgacccc gacgaggcga cgcgccaggc gcacggcgac gtcgtcctcg cgcgccagga
     2461 acgccggcac ggcgcagatc gtctcgaagc ggatgccggt ctcgggcgcc acggcgcgca
     2521 tcgccggctc gagcgaggcg gcgaaggcac gcacctcgcc ctgcatcgcc tcggcgtcgg
     2581 tgccgggcag gttgcggaac tcgtagttga aacggcaatc ggccgggacg atgttgtcgg
     2641 cgatgccgcc ctcgatgacg ccgaccgagc ccgtggtgta aggcacgtcg aagccttcgt
     2701 agcgcggcgc atcgtcgcga aagcgcgccg ccatgtcgct cagcttgccg acgacgcgcg
     2761 cgccgacctc gatggcgttg accgattgcg gcgtcagcga ggaatgcgcg gcgtggccgc
     2821 gaacgcagca gcgccagcga tagacgccct tgtgcgcgag ggcgggcacc atccccgtcg
     2881 gctcgccgac gatgcaggcg agcggcctca cgccccgctc ctgcagatcg gcgatcaggc
     2941 gcggcacgcc gaaggcaccg acctcctcgt cgtagctgaa ggcgaggtga acggaaaacg
     3001 gcaggtccgc ggagaggtag gcgggcacct gcgccaccgc caggccgatg aagctcttca
     3061 tgtcggcgct gccgcgaccg aagaggcgac cgtcgcgcag ctcggcgccg agcgggtcga
     3121 gcgtccagtc ctggccgtcc cagggcaccg tgtcggtgtg gcccgagagg atcacgccgg
     3181 ccggcttgcc ttcgccgagg gtggcgaaga ggttggcctt cttgcgctcg tcgtcgaacg
     3241 tcagcgtgct cgccacgcca tgacgcgcca gctcgtcgcg gatgaagtgg atcagctcga
     3301 ggttcgagtc gcggctgacc gtgcgcatgc gaaccagcga acgtgcgagc tcgagagcgt
     3361 gcgccgaagg cgcggccgac atgtccatcg acgcaatata gcggctgcag cctcgatccc
     3421 ctcgtccatg acgccggcat caaggccgca gtcatcgcgg atccgctcag cgtgtccttc
     3481 acgaggagat cccgacaccg cggacgtcga tcgcggggcg ttccacaaga gttcaaagcc
     3541 caggtgctcg acttcttccg gcggacgttg cgttgatcca cgagtcgcag cagggccggg
     3601 tttccgagtc acgcaccggg ggcgcctgcg ccgggccgtg gggatctggc acgatcacgc
     3661 cacgccgcgc tgatgtgggc gacagcgacg acggatgtcg cgaagacgac atgcggcgcc
     3721 accatcgcct tccggctgcc agagcgcaca tccgccgcgc cgccaccgac gtgctgacga
     3781 gaggatcgat tccatggatc gctggacctt ctacaccgcc cccggcacct gcgcgctggc
     3841 cacccacatc gcgctgcacg aggccggcgc cgcgttcgac gtggtcacgg tcgacttcgc
     3901 ggcccaccag cagcagagtc ctgcctacct cgcggtcaac ccgaaaggcc gcgtgccggc
     3961 gctcgccacg cccgcgggcg tcctcaccga aacgccggcc ctgctgctct tcgtcgcgca
     4021 gcgctttccc gaggcccgac tggcgccgct cgacgacccg ttcgcgctgg ctcggctgaa
     4081 cgagttcgac agctatctcg cgtcgaccgt gcacgtcgcg cacgcgcaca agcgccgcgg
     4141 ctcgcgctgg gccgacgacc cggcggcgca cgaggcgatg cgcgccaagg tgccgcagtc
     4201 gatgagtgcg tgcgctgccc tgctcgaggc gcgtctcgcg ccgggcccct gggtgctggg
     4261 cgagcgttac agcgtcgccg acggctacct gttcacgctc tctggctggc tcgaaggcga
     4321 tggcgtcgac atgaatcgct tcccgcgcct ggtggcgcac cgcgatcgcg ttgcgcaacg
     4381 cccggccgtg cagcgagccc tggccggggc gggcagctga tccggcgatc ggcggtcgcc
     4441 tgcttcggcg acgcggccac cggggtcgta cggcaaagtc gcagcagcgc gacgtcgccg
     4501 ttgtcaacgg catcaaacag cccacaacca tggccttcga tgctgtatcg ccacacgatg
     4561 tcttcaaggg cgcatcgtct tgtcgacgcc cttgcccatc gacagtacga tgcaaacgag
     4621 gagtccagcg atcgcggtcc tgagaaaacg atgacaagag tgtccgtcga ttcattgagc
     4681 tttaccgtcc gcgaggtcga ctcgccggag gacatggcca gggtctgcga actccgcgcc
     4741 gaatgctacg gtcgacgcgc acccgctctg cgcgagccca tggcgcatcc cgacgcattc
     4801 gacctctcac cgtcgacaac ggtgttcctg tgcgaggaca agacttccgg cgaactcgtc
     4861 ggaaccatgc gcgtcgtcga caacaccctc gacggcgatc tcgatatcga ggaattcatc
     4921 gaagtccccg gatggttgag cagcagcagg cggggcgaaa tgactcgact cgtcgtcgcg
     4981 cccggcgccg atccgctggt caaggcagcg ctgtggaagg caggttatct acgctgtctc
     5041 caggcgcgcg tgcaatggtt gatcatcgcc gcgcgcaagc ccggactcat caagcaatac
     5101 gagtatctgg gcgcgaagga cttgtacgcc gaccagcgcc tcgttcctgt cggtcgcggc
     5161 tggagttcgc cccaccgcat cttcgtcttc gacgtcctgt ccgcgaagga cgattggtac
     5221 gagggtcgtc accgcctcct gcgtttcatg gtcgagacgg agcaccccga catccgcctg
     5281 gcgtcaatgc acggcgccgc tgcgcccgct ccacggttga gtctcgtggc ctccgcctag
     5341 gtcgtcgttc tcgtcggcca ggagcgggat ccccggtccg agatgggcgg ccaggaccct
     5401 tgcgagacct tcatctgtaa atggcttgct caggaatcca tccatcccgg cagcctcgca
     5461 ggcgttgacg tcgtcgcggc tcgagttcgc ggtgagagcg atgatcggca gccgggatat
     5521 ccgtgccgcg cgttccctgg ccctgatttc gcgagtggcg ctcaggccgt cgagatcggg
     5581 catgcgcaag tccatgagga cggcgtgggg gcgctgtgag cccagtgctg ccgccacggc
     5641 tgcacggcca ctgctgacac gcgaaacctc tgcgccgagc tgcttcagca ccgcctcggc
     5701 gatgagggcg ttgacgtcgt tgtcttcggc cagcagcact cgatagccga caagcgaacg
     5761 aggggcggcc gacgagttgg cggccgcacc gtgagagtcg tcgtcctgag tgggcgcatc
     5821 gactgccggg agcaatgccc tgaactggaa cttcgaaccc acgccgacga tgctctcgac
     5881 cgtcacctcg cctcccatgg cgtgtgcaag ctcccgcgag atggccagac cgagccccgt
     5941 tccggcgtcg ggctgcgtgg cgacggaatc cgtttgcgca aacgcctcga agatccttgc
     6001 ctggtcgctg accgagatgc cggggcccgt gtcggtgacg gtacacacgg tctcgccttg
     6061 cttgcctcgc tccaccccga gtcggatcca gccctccttg gtgaacttca cggagttgcc
     6121 caacaggttg tgcaagacct gccttacgcg tggggcatcg cctagtaccc aacaggacga
     6181 cggcaactgg ccttcgagaa cgaaggcgac cgacgtcccg gaggtgcgca cccggtagag
     6241 ctcggcgagc tggcgcacct cgacagccag atcgaacggc gccagatgga gttcgagacg
     6301 gcccgtctcg atacgcgaga cgtcgagcaa tgcgccgatc agacccagaa ggtgctcacc
     6361 ttgcgcctgc acgatgccaa ggcgcgaaag cgataccggg tcctgatggt cgcgcttcat
     6421 catcgccgtg actccgagga t
//
LOCUS       DASRWS010000593         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1386228, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000593 DASRWS010000000
VERSION     DASRWS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386228"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFF72_11300"
     CDS             <1..768
                     /locus_tag="VFF72_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93795.1"
                     /translation="DPPHWSRWFRLVDTRSPTGTAVMKGPDDKPLLLLSREGKGRVAM
                     LLSDHIWLWARGYEGGGPQVDLLRRLSHWLMQQPDLEEEALRLVVHGKDLTVERQTMA
                     DSVDPVTVTSPTGATRTLTLQASEPGMWHSTIQANELGLWRATDGKLTALVNVGPANP
                     REFAQVTSTTDVLGPIANATGGDARRIADARGALEMPRILPVRSSDTYKGDDWLGVKM
                     RDASVVRGIGVLPVFAGLLGLLLLIGSLAATWAREGR"
     gene            complement(777..2126)
                     /locus_tag="VFF72_11305"
     CDS             complement(777..2126)
                     /locus_tag="VFF72_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZV93796.1"
                     /translation="MSFLVNIATAIVVHLRMWIEGIVAPFQIGAAVLAASGDGLKAKA
                     LTALGEPRVQRLGLRTARAFFPNLVLSRKFIGAYPNTGTAIVTRYQDVIEVLDRNADF
                     EVVYEPKMRLITGGENFFLGMQDTPLYTRDTSNMHLAMRREDVATIVAPLARRMADEI
                     VAGHAKSIDVPVDLTLAIPTAIVTDYLGVDDAPASDLVAWSTLMFWYLFIDLAGDQAL
                     AGKALDAAAACRAAIDRAVAARKASGVVKDDVLGRCLVLGKANLPGMDDLGIRNDLIG
                     LVIGAIPTISKACCQALDQLLDRPAALASARAAAQSGNDDVLAAHVFEAFRYNPINPL
                     IYRRAAVDATISSGSLRARTIPKGTMVLAVNLSAMFDPLKVASPEAFRTDRPWDDYII
                     WGYGMHACFGAYINRAVIPAILRPLLAKPGLRRAAGAAVGIDGGGTPFPQHFTVEWD"
     gene            complement(2168..>2642)
                     /locus_tag="VFF72_11310"
     CDS             complement(2168..>2642)
                     /locus_tag="VFF72_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HZV93797.1"
                     /translation="LRCPITSHMRRVNTRDGLAPTTDGGSVLNNRRRILRRGLPYGDA
                     SNGTPDSAEHGIIMLVVCASLFRQFEFVQQQWLNYGLDANAGNDTCPLVGNHATGGGR
                     EAKAKFVIASDPATGRPPFVVEGLPQFVETRGGEYFFVPSMTALRMIGMGVVDPT"
BASE COUNT          430 a          899 c          929 g          384 t
ORIGIN      
        1 gacccgccgc attggagccg ctggttccgg ctggtcgaca cgcgcagccc gaccggcacc
       61 gccgtgatga agggacctga cgacaagccg ctgctcttgc tgtcgcgcga gggcaagggc
      121 cgcgtcgcga tgctgctctc cgatcacatc tggctgtggg cgcgcggcta cgagggcggc
      181 ggaccgcagg tcgacctgtt gcggcgactc tcgcactggc tgatgcaaca gcccgacctc
      241 gaggaggaag cactgcgcct cgtggtgcac ggcaaggatc tgaccgtcga gcgccagacg
      301 atggcggaca gcgtcgatcc cgtaacggtc acgtcgccga ccggcgcgac ccgcacgctg
      361 acgctccagg ccagcgaacc gggcatgtgg cattcgacca tccaggcgaa cgagctcggc
      421 ctgtggcgcg cgaccgacgg caagctcacc gcgctggtca atgtcggccc cgccaacccg
      481 cgcgagttcg cccaggtgac gtcgacgacc gacgtgctcg gcccgatcgc caacgcgacc
      541 ggcggcgacg cgcgacgcat cgccgacgcg cgcggcgcgc tcgagatgcc gcgtatcctg
      601 ccggtgcgct cgagcgacac ctacaagggc gacgactggc tcggcgtgaa gatgcgcgac
      661 gcgagcgtcg tgcgcggcat cggcgtgctg ccggtgttcg cgggcctgct cgggctgctg
      721 ctgctgatcg gcagcctcgc ggcgacgtgg gcgcgggagg gacggtaagc gcccgctcag
      781 tcccactcca ccgtgaaatg ctgcgggaac ggcgtgccgc cgccgtcgat gccgacggcg
      841 gcgccggcgg cgcggcgcaa gccgggcttg gccagcagcg gcctgaggat cgccgggatg
      901 acggcgcgat tgatgtaggc gccgaagcag gcgtgcatgc catagcccca gatgatgtag
      961 tcgtcccagg ggcggtcggt gcgaaacgcc tccggcgacg cgaccttgag cggatcgaac
     1021 atcgccgaca ggttgaccgc cagcaccatg gtgcccttcg gaatcgtgcg cgcccgcagg
     1081 ctccctgacg agatcgtcgc gtcgaccgcg gcgcggcggt agatcagcgg gttgatcgga
     1141 ttgtagcgga acgcctcgaa cacatgcgcg gcgagcacgt catcgttgcc cgactgcgcc
     1201 gccgcccggg ccgacgccag cgccgccggc cggtcgagca gttgatcgag cgcctggcag
     1261 cacgccttgg aaatcgtcgg gatcgcaccg atgacgaggc cgatcaggtc gttgcgaatg
     1321 cccagatcgt ccatgccggg caggttggcc ttccccagca cgaggcaacg gccgagcaca
     1381 tcgtccttga cgacgcccga cgccttgcgg gccgcgaccg cgcgatcaat cgcggcgcgg
     1441 cacgccgccg cggcgtcgag cgccttgccg gccagtgcct gatcgccggc gagatcgatg
     1501 aacaggtacc agaacatcag ggtcgaccag gccaccagat cgctcgccgg cgcatcgtcg
     1561 acgccgagat agtcggtcac gatcgcggtc gggatcgcca gcgtgaggtc gaccgggacg
     1621 tcgatgctct tggcgtgccc ggcgacgatc tcgtcagcca tgcgccgggc gagcggcgcg
     1681 acgatcgtcg cgacgtcctc gcgccgcatc gcgagatgca tgttcgaggt gtcgcgggtg
     1741 tagagcggcg tgtcctgcat gccgagaaag aagttctcgc cgccggtgat caggcgcatc
     1801 ttcggctcgt acacgacctc gaaatcggcg ttgcggtcga gcacctcgat gacgtcctgg
     1861 tagcgagtga cgatcgcggt tccggtgttc ggataggcgc cgatgaactt ccgcgacagg
     1921 acgaggttcg ggaagaaggc gcgcgccgtg cgcaggccga gacgctgcac ccgcggctcg
     1981 ccgagtgccg tgagggcctt cgccttgagc ccgtcgccgc tcgccgccag gacggcggcg
     2041 ccgatctgga acggcgcgac gatgccctcg atccacatcc gcaagtggac cacgatcgcc
     2101 gtggcgatgt tgaccagaaa cgacatgcgc gccccctttg tcgagatccc ggcgagcaat
     2161 tcggcagtca ggtcggatcg acgacgccca tcccgatcat gcgcagcgcc gtcatgctcg
     2221 gcacgaagaa atattcccca ccgcgggtct cgacgaattg cggaaggccc tcgaccacga
     2281 agggcggccg ccccgtcgcc ggatccgacg cgatgacgaa cttggctttc gcctccctgc
     2341 cgccgccggt cgcatggttg ccgaccagcg gacaggtgtc gttgcccgca ttggcgtcga
     2401 ggccgtagtt gagccactgc tgctgaacga actcgaactg ccggaacagg ctggcgcaca
     2461 ccaccagcat gatgatgccg tgctcggccg agtcgggcgt gccgttcgag gcgtcgccgt
     2521 agggcagtcc gcggcggagg atgcgacgac gattgttgag caccgagccg ccgtccgtgg
     2581 tcggcgcgag cccgtcgcgc gtattcaccc gccgcatatg ggacgtgatc ggacaccgca
     2641 gg
//
LOCUS       DASRWS010000594         7781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1946820, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000594 DASRWS010000000
VERSION     DASRWS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946820"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFF72_11315"
     CDS             complement(<1..464)
                     /locus_tag="VFF72_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93798.1"
                     /translation="MSEAAATDEALLDVQDVHAGYGGSEVLHGVDLRIGRGEAVALLG
                     RNGMGKTTLVRTIAGLHRASAGRIAFAGAAVEREAPYRIARRGLGLVPEGRQVFANLT
                     VHEHLSAFERPAAQGSASNARIRIERVYTLFPRLNERRTHLGNQLSGGEQQML"
     gene            complement(461..1201)
                     /locus_tag="VFF72_11320"
     CDS             complement(461..1201)
                     /locus_tag="VFF72_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93799.1"
                     /translation="MATLNGDAPLLDVQRLAKRFGGVVASAGIDLQVRAGEVHALIGP
                     NGAGKTTLIAQLAGQLRPDGGRIVFAGDDITRLSPHQRARRGLARSFQITRLFRSFDV
                     QGNVALAVQASAASPMTRAAIEARAREVLAEIGLETRARDAIEALSHGEQRALEVGLA
                     VAGRPRLILLDEPMAGMGHEESRRMEALIARLRTQATLLIVEHDVDAVFRLADRVSVL
                     VGGSVIASGAPAAVRGDAAVIEAYLGEE"
     gene            complement(1189..2121)
                     /locus_tag="VFF72_11325"
     CDS             complement(1189..2121)
                     /locus_tag="VFF72_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93800.1"
                     /translation="MKARHLALAVVALLVLAVPPLLTAMGADYYVSTAGRIVVFAIAA
                     TSLNLVLGYGGMVSFGHAAFVGLGAYATGVMISEGHPDAFANLALTLAISAGAALVIG
                     AISLRTRGVYFIMITLAFGQMLFYLANSIKGYGGDEGLNIRARSALFGLDLKDPTTFY
                     YVALAVLAATLLALAAFVRSRPGRALVATRDDEQRAEALGFATYRLRLAVFVAAGAVA
                     GIAGALSVNQQGYVSPSLMQWTHSGTLMVMVILGGVASIWGGVLGAAVLVVAEDTLAS
                     YTTYWAFWTGWMLLIVVLFARRGLAGALDRAWRR"
     gene            complement(2336..3247)
                     /locus_tag="VFF72_11330"
     CDS             complement(2336..3247)
                     /locus_tag="VFF72_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93801.1"
                     /translation="MTLVLEQSLNGLQFGLMLFLLAAGLTLVFGVMDMINLAHGSLYM
                     VGAFIGAWLVGLTGSFTLGLVGAVIGTALFGMALETTLLRPLYARDHLSQVLATFALI
                     LILNDGTRMIFGNDLPLSAPAALAGPVQLVPGLGYPAYRLLIIGAGLAIAALLYVGVA
                     RTRMGALVRAGASNREMATAMGVDIRRLFTIVFGIGAGLCAVAGVLMGPLLAVQVGMG
                     ENILILAFVVIVIGGIGSIRGALVGALLVGFVDTLGRTVLQDAFRAFLPPQMASAAGP
                     AVASVAIYVFMAAVLAIRPQGLFPARG"
     gene            complement(3309..4496)
                     /locus_tag="VFF72_11335"
     CDS             complement(3309..4496)
                     /locus_tag="VFF72_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93802.1"
                     /translation="MFQRLSSTAAAVALAATFLAGPACAADKVKVGFVSTLSGPSAAL
                     GVDIRDGFLLAVKMNGGKLGGLPAEVIVGDDQFKPDVAKQLFEKDIKRDKVDFMTGVV
                     FSNIMLAALPEALDANVFYLSPNAAPSPMAGKDCNADFFAVSWPNDAYHEAAGAFANQ
                     RDLKSVYLMAPNYQAGKDSLAGFKRTYKGKVVGETYTKLGQLDYAAELAEVRAAKPQA
                     VYVFLPGGMGINFIKQFVGSGLSKDTTLLLPGFSADQDVIGPVGSAMSGIFNTAHWSP
                     DFSNPANVKFVEAFRKEYGRTPTLYASQGYDTANLINAAVHDVHGNLKDHAALHKALK
                     AARFDSVRGAFRFNTNQYPIQNYYVRVVSSDGHGGLVNKSFNEPILKDHGDAYVQDCK
                     MKP"
     gene            4654..4905
                     /locus_tag="VFF72_11340"
     CDS             4654..4905
                     /locus_tag="VFF72_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93803.1"
                     /translation="MKKILLATAILFSTAAAFAQAGTAIKETGKATAETAKQAKENVE
                     AAATKQPKKTMHKAKAKVHKASAAHHRAAAKAAAKEVGK"
     gene            complement(4995..5684)
                     /locus_tag="VFF72_11345"
     CDS             complement(4995..5684)
                     /locus_tag="VFF72_11345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZV93804.1"
                     /translation="MSGAKVKSEAAIPSAGADMGRLLAQDVVIGGEAAGPATGAQRME
                     PSHSPRATLAGQPAHERPLPRSESVAAADLHREVEQFLWRQAELLDGKHWQAWVDLFA
                     DDGIYWMPVAPEQTEWDGTPSIFVEDKSLMEIRKGRVSHPNAWSQAPHWETNHLVSHV
                     VVESSDRDTIAVRSRFHMMELRREVIRHFGGSYRHTLARDADGALRIKLQRVDLFNSQ
                     ASFDYVLQIWV"
     gene            complement(5681..6982)
                     /locus_tag="VFF72_11350"
     CDS             complement(5681..6982)
                     /locus_tag="VFF72_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZV93805.1"
                     /translation="MNPSALVEPDRVHKSVYTDQQIFDREMENIFEKVWVYCGHESQI
                     PKPGDYYAVTIGRQPMIMVRGADGKSIAVLHNRCPHRGVQLVGNLKGNTGGAFVCSYH
                     AWSFHLDGNVRAIPLARGYEGTAMNKSNPDCNIKPAPRVDSYRGFVFASLAAEGPTLL
                     EFLGDAKIAFDDMCDRSPLGEVEVVPYCHRMVQQSNWKFFMENQLDALHPSVTHQSTG
                     ISAGRVEKRIKEKDGHAPLHYHYLSTFASSFEQWDSVQTINFPHGHGILKAYMGLRPQ
                     DPDTLEYEALLRSAYGEAKTEEYLSRSIHHVLIYPYLSVQSPLQQLRCLRPISPDKTL
                     SEIWHFRLKGAPEAIYRRSLWYFNLVNSPATMINADDLENWTKAQWGLASDGGDWVSF
                     HRNHGDDRIENGVAYSNHGTSEVVMRSQFKAWAKYMQGDAA"
     gene            complement(7070..>7781)
                     /locus_tag="VFF72_11355"
     CDS             complement(7070..>7781)
                     /locus_tag="VFF72_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZV93806.1"
                     /translation="DNHGQVLGVRRKLMPTFVERAVWGCGDGSDLLSFDTEFGRIGGL
                     ICGENLMTLARAHLIAQGEDFHVAVFPGAFALHTGPKLEEFDASAASFWGYSSCRAHA
                     FEAGAFVIGSCGYITQEDIPGDFALRDTLNVDYARGGSAIYAPIGIPLAPPTPGDAIV
                     YAVCPAEFVKIAKAVVDTVGHYARPDLFALTVTAPAHQRLVKRRAVESIPAGELLRIA
                     DRHEVRVADIEASVTATG"
BASE COUNT         1239 a         2603 c         2785 g         1154 t
ORIGIN      
        1 agcatctgct gctcgccgcc ggagagctga ttgccgaggt gcgtgcgccg ctcgttcagt
       61 cgcgggaaga gcgtgtagac gcgctcgatg cggatgcgcg cgttgctcgc cgacccttgc
      121 gcggccggcc gctcgaaagc gctcaggtgc tcgtgcaccg tgaggttcgc gaacacctgt
      181 cttccttctg gcacgaggcc gaggccgcgc cgggcgatgc ggtacggtgc ttcgcgttcg
      241 acggcagcgc cggcgaaggc gatgcggccg gcgctggccc ggtgcagccc ggcgatcgtg
      301 cgcacgagcg tcgtcttgcc catgccgttg cggccgagca gcgcgaccgc ttcgccgcgg
      361 ccgatccgca ggtcgacgcc gtgcagcacc tcgctgccgc cgtagccggc gtgcacgtcc
      421 tgcacgtcga gcagcgcctc gtcggtggca gcggcttcgc tcattcctcg cccaggtagg
      481 cctcgatgac ggcggcgtcg ccgcgcacgg ccgccggtgc gccgctggcg atgacgctgc
      541 cgccgaccag caccgagacg cggtcggcga gccggaacac ggcgtcgaca tcgtgctcga
      601 cgatgagcag cgtcgcctgc gtgcgcagcc gcgcgatcag cgcttccatg cgccgcgact
      661 cctcatggcc catgccggcc atcggctcgt cgagaaggat gagccgcggc cgcccggcga
      721 cggcaaggcc cacctccagc gcgcgctgct cgccgtgcga gagcgcctcg atcgcatcac
      781 gcgcgcgcgt ctcgaggccg atctcggcga gcacctcgcg ggctcgtgcc tcgatcgccg
      841 cccgggtcat cggtgacgcg gcggacgcct gcacggcgag ggcgacgttg ccctgcacgt
      901 cgaagctgcg aaagaggcgc gtgatctgga acgagcgcgc caggccgcgc cgcgcccgct
      961 ggtgcggcga aaggcgcgtg atgtcgtcgc cggcgaagac gatgcggccg ccgtcgggtc
     1021 gcagctggcc ggcgagctgg gcgatcagcg tcgtcttgcc ggcgccgttg gggccgatca
     1081 gcgcgtgcac ctcgccggca cgcacctgca ggtcgatgcc ggcgctggcg accacaccgc
     1141 cgaagcgctt ggccagccgc tgcacgtcga gcagcggcgc gtcgccgttc agcgtcgcca
     1201 tgcgcggtcc agtgcgccgg caagaccgcg gcgcgcgaag aggacgacga tcagcagcat
     1261 ccagccggtc cagaacgccc agtacgtcgt gtacgacgcg agcgtgtcct cggcgacgac
     1321 gagcaccgcc gcgccgagga cgccgcccca gatcgaggcg acgccgccga ggatcaccat
     1381 caccatcagc gtgcccgaat gcgtccactg catcagcgac gggctgacat agccctgctg
     1441 gttgaccgag agtgcgccgg cgattccggc gacggcgccg gcggcgacga agacggccag
     1501 gcgcagccgg taggtcgcga agcccagcgc ttcggcgcgc tgttcgtcgt cgcgcgtcgc
     1561 gacgagggcg cggccgggcc gcgaacgaac gaaggcagcg agcgccagca gcgtggcggc
     1621 gagcacggcg agagccacgt agtagaacgt cgtcgggtcc ttcaggtcga ggccgaacaa
     1681 ggccgagcgg gcgcggatgt tgaggccctc gtcgccgccg tagcccttga tcgagttggc
     1741 caggtagaag agcatctggc cgaaggccag cgtgatcatg atgaagtaga cgccgcgcgt
     1801 gcgcagcgag atcgcgccga tgacgagcgc cgcgccggcc gagatcgcga gggtgagcgc
     1861 gagattggcg aaggcgtcgg ggtggccttc gctgatcatc acgccggtgg cgtaggcgcc
     1921 gaggccgacg aacgcggcgt gcccgaacga gaccatgccg ccgtagccga ggacgaggtt
     1981 gaggctcgtc gccgcaatcg cgaacacgac gatgcgcccg gcggtgctca cgtagtagtc
     2041 ggcgcccatc gccgtgagaa gcggcggtac ggcgagcacg agcaacgcga cgacggcaag
     2101 cgccagatga cgcgccttca tcgcgccccc tcgggaggca gcgaacgtac tcgtgagcgt
     2161 gggggcacca ccggccggac gccgggccgc cccaaggccg gccgcgcccc ctcggggggc
     2221 agcgaacgta cccgtgagcg tgggggcacc accggccggg cgccgggccg ccccaaggcc
     2281 ggccgcgccc cctcgggggg cagcgaacgt actcgtgagc gtgggggcac caccttcacc
     2341 cgcgagccgg gaacaggccc tgcggccgga tcgccagcac cgcggccatg aagacgtaga
     2401 tcgccaccga ggccaccgcg ggcccggcgg cgctcgccat ctgcggcggc aggaaggcgc
     2461 gaaacgcgtc ctgcagcacg gtgcggccga gcgtgtcgac gaagccgacc agcaacgcac
     2521 cgacgagggc gccgcggatc gagccgatgc cgccgatgac gatgacgacg aaggcgagga
     2581 tgaggatgtt ctcgcccatg ccgacctgca ccgcgaggag cggccccatc agcacgccgg
     2641 ccaccgcgca caggcccgcg ccgatgccga agacgatcgt gaagaggcgc cggatgtcga
     2701 cgcccatcgc cgtcgccatc tcgcggttcg acgcgccggc gcggaccagc gcgcccatcc
     2761 gcgtccgcgc caccccgacg tagagcagcg ccgcgatcgc caggccggcg ccgatgatga
     2821 ggagccggta ggccggatag ccgagccccg gcaccagctg caccggaccg gccagcgccg
     2881 ccggcgccga cagcggcagg tcgttgccga agatcattcg cgtgccgtcg ttgaggatca
     2941 ggatcagcgc gaacgttgcg agcacctgcg acaggtggtc gcgcgcatag agcggacgca
     3001 gcagcgtcgt ctcgagcgcc atgccgaaga gggcggtgcc gatcaccgcg ccgacgaggc
     3061 cgagcgtgaa cgagccggtc aggcccacca gccaggcacc gatgaaggcg cccaccatgt
     3121 atagcgagcc gtgcgccagg ttgatcatgt ccatgacgcc gaagaccagc gtcaggccgg
     3181 cggcgagcag gaacagcatc agcccgaact gcaagccgtt gaggctctgc tcgagaacga
     3241 gcgtcacgac aagcccgtgc cgtgacggcc cgccgtggcg ggcgagggcg acctcgccgc
     3301 cgccagcctc acggcttcat cttgcagtcc tgcacgtagg cgtcgccgtg gtccttcagg
     3361 atcggctcgt tgaacgactt gttgacgagg ccgccgtggc cgtcgctgct gacgacgcgc
     3421 acgtagtagt tctggatcgg gtactggttg gtgttgaagc ggaaggcgcc gcgcaccgag
     3481 tcgaaacggg ctgccttcag cgccttgtgc agggccgcgt ggtccttcag gttgccgtgc
     3541 acgtcgtgca cggcggcgtt gatcaggttg gcggtgtcgt agccctgcga ggcgtagagc
     3601 gtcggcgtgc ggccgtactc cttgcggaac gcctcgacga acttgacgtt ggccggattg
     3661 ctgaagtcgg gcgaccagtg cgcggtgttg aagatgcccg acatcgccga accgaccggg
     3721 ccgatcacgt cctggtcggc cgagaagccg ggcagcagca gcgtcgtgtc cttggacagg
     3781 cccgagccga cgaactgctt gatgaagttg atgcccatgc cgcccggcag gaacacgtag
     3841 acggcctgcg gcttggcggc gcgcacctcg gcgagctcgg ccgcgtagtc gagctggccg
     3901 agcttggtgt acgtctcgcc gacgaccttg cccttgtacg tgcgcttgaa gccggcgagc
     3961 gaatccttgc cggcctggta gttgggcgcc atcaggtaca cgctcttcag gtcgcgctgg
     4021 ttggcgaacg cgccggcggc ctcgtgatag gcgtcgttcg gccacgagac ggcgaagaag
     4081 tcggcgttgc agtccttccc cgccatcggc gagggcgcgg cattcgggct caggtagaag
     4141 acgttggcat cgagcgcttc gggcagcgcc gccagcatga tgttggagaa gacgacgccg
     4201 gtcatgaagt cgaccttgtc gcgcttgatg tccttctcga agagctgctt ggccacgtcg
     4261 ggcttgaact ggtcgtcgcc gacgatcacc tcggccggca ggccgccgag cttgccgcca
     4321 ttcatcttca cggcgagcag gaagccgtcg cggatgtcga cgccgagcgc tgccgacggg
     4381 cccgagagcg tgctgacgaa gccgaccttg accttgtcgg cggcgcaggc cggcccggcc
     4441 aggaacgtcg cggcgagggc gacggcggcg gccgtcgacg aaaggcgctg gaacatgacg
     4501 gtaaaccttt cagggacgtg gatcggagcc ggatggctgc cgaattgtag ggaccacggt
     4561 gtcaacctcg aaagcgttcg tcgcgttgtg gagcggcgcc ctcgcgccgc ggggccgcgc
     4621 agcccgaccc actcatccga gaaacgaccc atcgtgaaga agatcctcct ggccacggcc
     4681 atcctgttct ccaccgccgc tgccttcgcg caggccggca ccgccatcaa ggaaaccggc
     4741 aaggccaccg ccgaaaccgc gaagcaggcg aaagaaaacg tcgaagccgc cgcgaccaag
     4801 caaccgaaga agacgatgca caaggccaag gccaaggtcc acaaggccag cgctgcgcat
     4861 caccgcgctg ccgccaaggc cgcggcgaag gaagtcggca agtaattccc ggttggcggg
     4921 gggcgctcga cgccgccccc tgatccgcgc cttgcgacat ccgggcgcgt ggcgccccgc
     4981 agtgtcggcg ggcctcagac ccagatctgc agcacgtagt cgaatgacgc ctggctgttg
     5041 aagaggtcga cgcgctgcag cttgatgcgc agcgcgccgt cggcgtcgcg cgcgagcgtg
     5101 tgccggtagc tgccgccgaa atggcggatc acctcgcgtc gcagctccat catgtggaag
     5161 cgtgagcgca cggcgatcgt gtcgcggtcg ctcgactcga cgacgacatg gctgacgagg
     5221 tggttcgtct cccagtgcgg cgcctgcgac caggcgttcg ggtgcgacac gcgccccttc
     5281 ctgatctcca tcaggctctt gtcctcgacg aagatcgagg gcgtgccgtc ccactcggtc
     5341 tgttccggcg ccaccggcat ccagtagatg ccgtcgtcgg cgaagaggtc gacccacgcc
     5401 tgccagtgct tgccgtcgag cagctcggcc tgccgccaga ggaactgctc gacctcgcgg
     5461 tgcaggtcgg ccgcggcgac gctctcgctg cgcggcaacg gccgctcgtg cgccggctgt
     5521 ccggccagcg tggcgcgcgg cgagtggctg ggttccatgc gctgcgcgcc ggtggcgggc
     5581 cccgcggcct cgccgccgat gacgacgtct tgcgccagca ggcgccccat gtcggcgccg
     5641 gcgctcggga tcgccgcttc gctcttcacc ttggcgccgc tcatgcggcg tctccctgca
     5701 tgtacttcgc ccaggccttg aactggctgc gcatcaccac ctccgaggtg ccgtggttcg
     5761 agtaggcgac gccgttctcg atccggtcgt cgccgtggtt gcggtgaaag ctcacccagt
     5821 cgccgccgtc ggacgccagg ccccattgcg ccttggtcca gttctcgagg tcgtcggcgt
     5881 tgatcatcgt cgccggcgag ttgacgaggt tgaagtacca gaggctgcgc cggtagatcg
     5941 cctcgggcgc gcccttcagg cggaagtgcc agatctcgga aagcgtcttg tcgggcgaga
     6001 tcgggcgcag gcaccgcagc tgctgcagcg gcgactgcac cgacaggtac ggatagatca
     6061 gcacgtggtg gatgctgcgc gacaggtact cctcggtctt cgcctcgcca taggctgatc
     6121 tcagcagcgc ctcgtactcg agcgtgtcgg ggtcctgcgg gcgcaggccc atgtaggcct
     6181 tgaggatgcc gtggccgtgc gggaagttga tcgtctgcac gctgtcccac tgctcgaagc
     6241 tcgaggcgaa cgtcgagagg tagtggtagt gcagcggcgc gtggccgtcc ttttccttga
     6301 tgcgcttctc gacccggccg gccgagatgc cggtcgactg gtgcgtcacc gaggggtgga
     6361 gcgcgtcgag ctggttttcc atgaagaact tccagttcga ctgctgcacc atgcggtggc
     6421 agtagggcac gacctccact tcgccgagcg gcgagcggtc gcacatgtcg tcgaaggcga
     6481 tcttggcgtc gccgagaaac tccaggagcg tcggcccctc ggcggcgagg ctggcgaaga
     6541 cgaagccgcg atagctgtcg acgcgcggcg ccggcttgat gttgcagtcg gggttcgact
     6601 tgttcatcgc cgtgccctcg tagccgcgtg ccagcggaat ggcgcgcacg ttgccgtcga
     6661 ggtggaagct ccaggcgtgg tacgagcaga cgaaggcgcc gccggtgttg cccttgaggt
     6721 tgccgacgag ttgcacgccg cggtgcgggc agcggttgtg cagcaccgcg atgctcttgc
     6781 catcggcccc gcgcaccatg atcatcggct ggcggccgat ggtgacggcg tagtagtcgc
     6841 cgggtttcgg gatctgcgat tcgtggccgc aatagaccca gaccttctcg aagatgtttt
     6901 ccatctcccg gtcgaagatc tgctggtcgg tatagacgga cttgtggacg cggtcgggtt
     6961 cgacgagggc gctgggattc atcggaggct cggtcttcag gcaggcgtgc gggccgggga
     7021 aagcgagtct agtgcggtcg caaccctgcc tccccggagc ggaagaattt cagccggtgg
     7081 ccgtgacgct cgcctcgatg tcggcgacgc gcacttcatg ccggtcggcg atgcgcaaga
     7141 gctcgccggc ggggatcgac tcgacggcgc gtcgcttcac aagccgctga tgcgcgggtg
     7201 cggtgacggt gagagcgaac aggtcgggcc tcgcgtaatg accgacggtg tctacgacgg
     7261 ccttggcgat cttgacgaac tcggccgggc acaccgcgta gacgatcgca tcgccgggtg
     7321 tcggcggcgc gagcgggatg ccgatcgggg cgtagatcgc gctgccgccg cgggcgtagt
     7381 cgacgttgag cgtgtcgcgc agtgcgaagt cgcctggaat gtcttcctgg gtgatgtagc
     7441 cgcacgagcc gatcacgaag gcgcccgcct cgaacgcgtg ggcgcggcag ctcgaatagc
     7501 cccagaagct cgccgcggac gcatcgaatt cttcgagctt cgggccggta tgcagtgcga
     7561 acgcgccggg aaagacggcg acgtggaaat cttccccttg cgcgatgaga tgggcccgcg
     7621 caagcgtcat caggttttcg ccgcagatca gaccgccgat gcggccgaac tcggtgtcga
     7681 aggaaagcag atccgaaccg tcgccgcaac cccagaccgc acgctccacg aacgtcggca
     7741 tgagcttgcg ccgtacgccg agaacctgcc catggttgtc g
//
LOCUS       DASRWS010000595         6617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2827408, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000595 DASRWS010000000
VERSION     DASRWS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827408"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..906
                     /locus_tag="VFF72_11360"
     CDS             79..906
                     /locus_tag="VFF72_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZV93807.1"
                     /translation="MKARTLLGAASTALFAAMLPLSAHAVDTVTINYFTIASTDQDAN
                     HLGGGTVDNEVQTHLGPNGLPVLNTSAYGCTSNCFTLGPPTDVLSTGEITYWSPALNN
                     GGAGGTSDVMATGSANVTLPFSVPTDFFPPNGIGSGDGGNNGYLAAVLSGTLNAPGTE
                     LISFSIGADDMAFAYLDGVNVCDLGGVHPATTGTCVTPFDIAAGPHDLRVFFVDINQV
                     QSGFTFGLNTQGVTIQPAVPEPSTYALFGLGLLGLGAMVRRRKSRRGEGSAQRLALA"
     gene            1085..2278
                     /locus_tag="VFF72_11365"
     CDS             1085..2278
                     /locus_tag="VFF72_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZV93808.1"
                     /translation="MQPRIPGFRLLHSPGPTHVPDAVVAAMSRQPTDLADPRVATLVE
                     RCEAGMKTLLGCGRAELFFYSANGHGGWEAVIANLVAPGSTILVPGTGHFSDSWAEQV
                     EAFGGTALRTPYREGFPIDPAVVEAALRDDREHRIGAVFAVHTDTASGTTSDLQALRR
                     AIDAAGHPALFVVDVVASLGAAPFAMDALGVDAVMGASQKGLMLQPGMAFVAAGARAL
                     AAARGNPTPRYYWDWERRVSEHSYRKFCGTPPLAHLAGLAAALELIEDEGLDQVHARH
                     ARLARAVHAAVGRWAEAGAVRLFTRDAAARSVSVTAVETSPGVDPEALRAVARERFQV
                     AIAGGLGPLSGRVFRIGHLGDMNEAMVLGCLAGVEAAMRVQGVAHGPHGVDAAIDSLA
                     RSTAG"
     gene            2382..3101
                     /locus_tag="VFF72_11370"
     CDS             2382..3101
                     /locus_tag="VFF72_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93809.1"
                     /translation="MGASEAAWTVRPHGKLVEVDDGILTVTGEVEMPLMTLQRRMTVV
                     ALAGRRWLAWSAIALDETEMAVIGRFGRPAFLVVPNDHHRLDARRWKARFPELQVVAP
                     EGARDKVAEAVLVDTTMPDFDDPAVRFVVVAGTRGHEAALLVDRADGTTLILNDLVGN
                     IRTEHGFGGWLLRLMGFAGDEPHIPAPVRMMLVKDTDALRAQLLEWAALPSLRRIVVS
                     HGATIEEHAGDALRALAASLK"
     gene            3249..4112
                     /locus_tag="VFF72_11375"
     CDS             3249..4112
                     /locus_tag="VFF72_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93810.1"
                     /translation="MLTLLFDGVAYGMLLFVLSVGLAVTLGLMNFINLAHGAFAMAGG
                     YITVMLMNKLGVPFLACLPLAFLGSALLGALLEPTLYRPMYAKPHLDQVLFSIGLVFM
                     AVTAVDFFVGSMQQNITLPMWLQGRLPIAAVGIGRYRLFIIVICALLTVALQWVLSRT
                     RFGSRLRAAVDDPRTAGGLGLDVNRIFLLTFAVGSGLAGLGGALGAEVLGLDPTFPLK
                     FMIYFLIVCSVGGTTTITGPLLAALLLGIFDVAGKYYVPKLGAFFVYTLMIVILVVRP
                     QGLFARAGGRA"
     gene            4109..5068
                     /locus_tag="VFF72_11380"
     CDS             4109..5068
                     /locus_tag="VFF72_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93811.1"
                     /translation="MTAASSLRHRARWRWWELALFAALPLSWWLLPAQSLLLNEIAIT
                     ALFAVSLDLILGYAGIVSLGHAAFFGFGAYAAALFAKDVMPEPLLGLAFAIVASALLG
                     LVTSVLILRGTDLTRLMVTLGVASILYELANKLDWLTGGADGLQGVVMAPLVGRFPFD
                     LAGRTAFAYSLVVLAAALFVARRIVHSPFGLSLQALRDNRLRASSIGLSVRLRLVAVY
                     TLSAAIAGAAGGLLAQTAGFASLDLFDFQRSADVLLVLVIGGTGYLYGGIFGAIVFKL
                     LHDLVSDWTPQYWNFWLGLFLVVLVMVGRDRLIRPWTWFRSAR"
     gene            5065..5835
                     /locus_tag="VFF72_11385"
     CDS             5065..5835
                     /locus_tag="VFF72_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93812.1"
                     /translation="MTAVLQTRTLVKRFGGLAATSDVSIAVERGARHALIGPNGAGKT
                     TLINLLTGVLEPTSGAIALDGVDITRLSVHERVRRGIVRTFQINQLFGGLTPLTSLAL
                     AVSVRRGKSAGWWRPVGSDAAVAAECDSLLERFRLAEVMDQPVHALAYGKRRLLEIAT
                     ALACQPRVLLLDEPVAGVPEGDRQEIFETINALPGDVSILLIEHDMDLVFNFARFVTV
                     LVNGAVFAEGDVASISADPRVKAVYLGEDEARAGAPGG"
     gene            5828..6535
                     /locus_tag="VFF72_11390"
     CDS             5828..6535
                     /locus_tag="VFF72_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93813.1"
                     /translation="MAELLRVEALRAGYGEAVVVQGVDLVLAEGESMALLGRNGTGKT
                     TLLNTLVGVTRRHGGRIALGGRDVTTLAPHARAAAGIGWVPQERNIFRSLSVDENLTA
                     VARPGPWTPARVYAMFPRLAERRANMGSQLSGGEQQMLAVARALVLNPKLLLLDEPLE
                     GLAPIIVKELLASIATVVRDEGVSAIVVEQNPRQILPITSRAVILDRGAIVDEGSSAE
                     LLADRERLDRWLAVARA"
BASE COUNT          854 a         2405 c         2225 g         1133 t
ORIGIN      
        1 cgcacccctt gttcgcggga tggcgtggat cggccgacgg cgcatgctcc atcgcttgcg
       61 ctttgcgagg agatgtccat gaaagcacgc acgctgctcg gagcggcctc cacggcgctg
      121 ttcgcagcga tgttgccgct ctcggcccac gccgtcgaca cggtcaccat caactacttc
      181 accatcgctt ccaccgacca ggacgccaac cacctcggcg gcggcacggt cgacaacgag
      241 gtgcagaccc atctcgggcc gaatggcctg ccggtgctca atacgtcggc ctacggctgc
      301 acctcgaact gcttcacgct cggcccgccg accgacgtcc tgtccaccgg cgagatcacc
      361 tactggagcc cggcactcaa caacggcggt gccggcggca cctcggacgt catggcgacg
      421 ggaagcgcca atgtcacgct gcccttcagc gtgcccaccg atttctttcc tccgaacggc
      481 atcggctcgg gcgacggcgg aaacaacggc tatctcgccg ccgtcctttc gggcactctc
      541 aatgcgccgg gtaccgagct gatcagcttc tcgatcggcg ccgatgacat ggccttcgcc
      601 tatctcgacg gggtcaatgt ctgcgacctg ggcggggtcc atcccgcgac caccggaacc
      661 tgcgtcacgc cgttcgacat cgccgcgggc ccgcacgacc tgcgcgtctt cttcgtcgac
      721 atcaaccagg ttcagtccgg cttcacgttc gggctgaaca cgcagggcgt gacgatccag
      781 ccggcggtgc cggagccttc gacctacgcg ttgttcggcc tcggcctgct cggcctcggc
      841 gccatggtgc gccggcgcaa gagccggcgc ggcgagggct cggctcagcg actcgcgctc
      901 gcctgacgcc gagccgccga cgcggctcaa cctcgccgac gcaccggtgt cggcattgac
      961 acctcgcccc tcgcgacccg aggggcgatc ttcttgcgct ctcgccggcg cctccattcg
     1021 gctcgcttcg catcgggtcg cgagcggccg atgccgcatt cggtgcggct accatctccg
     1081 caccatgcag ccacgcattc ccggatttcg cctccttcat tcaccggggc cgacccacgt
     1141 gcccgacgcc gtcgtcgcgg cgatgtcgcg acagccgacg gatctcgccg atccgcgcgt
     1201 cgcgacgctc gtcgagcgct gcgaagcggg gatgaagacg ctgctcggct gcgggcgggc
     1261 cgaactgttc ttctattccg cgaacggcca tggcggctgg gaggcggtga tcgccaacct
     1321 cgtcgccccg gggtcgacga tcctcgttcc gggcaccggc catttttccg acagctgggc
     1381 cgagcaggtc gaggccttcg gcggcacggc actgcgcacg ccgtaccgtg agggctttcc
     1441 catcgatccg gcggtcgtcg aggcggcctt gcgcgacgac cgcgagcacc gcatcggcgc
     1501 ggtgttcgct gtccacaccg acacggcgag cggcacgacc agcgacctgc aggcactgcg
     1561 tcgcgccatc gatgcggccg ggcacccggc gctgttcgtg gtcgacgtcg tcgcctcgct
     1621 cggcgccgcg ccgttcgcga tggacgcgct cggtgtcgac gccgtgatgg gcgcctcgca
     1681 gaagggcctg atgctgcaac ccggcatggc cttcgtcgcc gccggtgcgc gggcccttgc
     1741 cgcggcgcgc ggcaacccga cgccgcgcta ctactgggac tgggagcggc gcgtcagcga
     1801 gcactcgtac cgcaagttct gcggcacgcc gccgctcgcc catctggccg gcctggcggc
     1861 ggcgctcgag ctgatcgaag acgaagggct cgaccaggtc cacgcccgac acgcgcgcct
     1921 ggcccgggcc gttcacgccg ccgtcggtcg ctgggccgag gccggcgcgg tccgcctctt
     1981 cacccgcgat gccgcggcgc gctcggtctc ggtgaccgcg gtcgagacct cgcccggcgt
     2041 cgatcccgag gccctgcgcg cggttgcccg ggagcgcttc caagtcgcga tcgccggcgg
     2101 tctcggcccg ttgtcgggac gcgtcttccg gatcggccac ctcggcgaca tgaacgaggc
     2161 gatggtgctc ggctgcctcg ccggcgtcga ggcggcgatg cgcgtccagg gcgtcgccca
     2221 cggcccccac ggcgtcgacg cggcgatcga ttcgctggct cgctcgaccg ccggctgacc
     2281 tgcgtccccg ggggcggccg ccgcgaaccg cgccgggcgc gccacttgcc gctcgccccc
     2341 tgcgccatcc tttttcgggc gcggcgatga caggagaggc gatgggtgca agcgaagcgg
     2401 catggacggt gcgtccgcac ggcaagctgg tcgaggtcga tgacggcatc ctcaccgtca
     2461 ccggcgaggt cgagatgccg ttgatgacgc tgcagcggcg gatgacggtg gtggccctgg
     2521 ccggccgccg atggctggcg tggagcgcga tcgcgctcga cgaaaccgag atggcggtga
     2581 tcgggcgatt cggccgcccc gcgttcctgg tcgtgcccaa cgaccaccat cgtctcgatg
     2641 cccggcgatg gaaggcgcgc tttcccgagc ttcaggtcgt cgcgcccgaa ggcgcccgcg
     2701 acaaggtcgc cgaggcggtg ctcgtcgaca cgacgatgcc cgacttcgac gatccggcgg
     2761 tgcgcttcgt cgtcgtcgcg ggcacgcgcg ggcacgaggc ggctctcctc gtcgatcgcg
     2821 ccgatggcac cacgttgatc ctcaacgacc tcgtcggcaa catccgcacc gagcacggat
     2881 tcggcggctg gctgcttcgc ctgatgggct ttgccggcga cgagccgcat atcccggcgc
     2941 cggtcaggat gatgctcgtc aaggacaccg acgcgcttcg cgcgcaactg ctcgagtggg
     3001 ccgccctgcc atcgctcagg cgaatcgtcg tttcgcacgg agcgacgatc gaggagcatg
     3061 ccggcgacgc gctgcgcgcg ctcgcggcgt cgttgaagta gggcaggtcg accgcagcgt
     3121 accggcgccg gccgcggcgt cggagccgac gcgggcgtgt cgtcgacacg cgatgacgtc
     3181 gggaagtcgc gcgggccgcg gccgcgctcg atgaccgaga atcgcgctcc ggatcccatg
     3241 caccttccat gctgaccctg ctcttcgacg gcgtcgccta cggcatgctg ctgttcgtgc
     3301 tgtcggtggg actggcggtg acgctggggc tgatgaactt catcaacctc gcgcacggcg
     3361 ccttcgcgat ggccggcggc tacatcacgg tcatgctgat gaacaagctc ggcgtgccgt
     3421 ttctcgcctg cctgccgctc gccttcctcg gttcggcgct gctcggcgcg ttgctcgagc
     3481 cgaccctgta ccgtcccatg tacgccaagc cgcatctcga ccaggtgctg ttctcgatcg
     3541 gcctggtctt catggccgtc accgccgtcg acttcttcgt cggctcgatg cagcagaaca
     3601 tcacgctgcc gatgtggctg cagggacgcc tgccgatcgc cgcggtcggc atcggccgct
     3661 accggctctt catcatcgtc atctgcgcgc tgctgacggt ggcgctgcag tgggtgctgt
     3721 cgcgcacccg cttcggcagc cggctgcgag cggccgtcga cgacccgcgc accgccggcg
     3781 gcctcggcct cgacgtcaac cgcatctttt tgctcacctt cgcggtcggc tccggtctgg
     3841 ccggcctcgg cggcgcgctc ggtgccgaag tgctcggcct cgatccgacc ttcccgctca
     3901 agttcatgat ctatttcctg atcgtctgct cggtcggcgg gacgacgacg atcaccggcc
     3961 cgttgctcgc ggccctcctg ctcggcatct tcgacgtcgc cggcaagtac tacgtgccca
     4021 agctcggcgc gttcttcgtc tacaccctga tgatcgtcat cctggtggtg cgaccgcaag
     4081 gcctgttcgc ccgcgccggc ggccgcgcat gacggcggcg tcgtcgctgc gccatcgcgc
     4141 gcgctggcgc tggtgggagc tggcgttgtt cgccgcgctg cccctctcgt ggtggctcct
     4201 gccggcgcag tcgctgctgc tcaacgagat cgcgatcacg gctctcttcg ccgtctcgct
     4261 cgacctgatc ctcggctacg ccggcatcgt ctcgctcggc cacgccgcct tcttcggctt
     4321 cggcgcgtat gcggcggcgc tgttcgccaa ggacgtgatg cccgagccgc tcctgggcct
     4381 ggcgttcgcc attgtcgcca gcgcgttgct cggcctggtg accagcgtgc tcatcctgcg
     4441 cggtaccgac ctgacgcgcc tgatggtgac gctgggcgtg gcctcgatcc tctacgagct
     4501 tgccaacaag ctcgattggc tgaccggcgg cgccgacggc ctgcagggcg tcgtcatggc
     4561 gccgctcgtc ggccgcttcc cgttcgacct cgccggccgc accgccttcg cctattcgct
     4621 cgtggtgctg gccgcggctc tgttcgtggc gcgacgcatc gtccattcgc cgttcggcct
     4681 gtccttgcag gcgctgcgcg acaaccggct gcgcgcttcc agcatcggcc tgtcggtgcg
     4741 gctgcggctc gtcgcggtct acaccctgag cgcggcgatc gccggcgccg cgggcggcct
     4801 gctggcgcag acggcgggat tcgcctcgct cgacctgttc gacttccagc gcagcgccga
     4861 cgtcctgctc gttctcgtga tcggcggaac gggctatctc tacggcggca tcttcggagc
     4921 gatcgtcttc aagctgctgc acgatctcgt ttccgactgg acgccgcagt actggaattt
     4981 ctggctcggc ctgttcctgg tcgtgctcgt gatggtcggc cgcgatcgcc tgatccggcc
     5041 gtggacgtgg tttcggagcg cacgatgacc gccgtcctgc agacgcgcac gctggtcaaa
     5101 cgtttcggcg gcctggccgc gacgagcgat gtctcgatcg ccgtcgagcg cggcgcgcgg
     5161 cacgccctga tcgggccgaa cggcgccggc aagacgacgc tgatcaacct gctgaccggc
     5221 gttctcgagc cgacctccgg agcgattgcg ctggacggcg tcgacatcac gcgcctgtcg
     5281 gtccacgagc gggtgcgccg cggcatcgtc cggaccttcc agatcaacca gctcttcggc
     5341 gggctgacgc cgctgacgtc gctcgccctg gccgtctcgg tgcggcgcgg caagagcgcg
     5401 ggctggtggc gacccgtcgg cagcgacgcg gcggtcgctg ccgaatgcga cagcctgctc
     5461 gagcgatttc gcctcgccga ggtgatggac cagccggtgc acgcgctcgc ctacggcaag
     5521 cggcggttgc tcgagatcgc gactgcgctc gcctgccagc cgcgcgtcct gctgctcgac
     5581 gaaccggtcg ccggcgtgcc cgagggcgac cgccaggaga tcttcgagac gatcaacgca
     5641 ttgccgggcg atgtctcgat cctgctcatc gagcacgaca tggacctcgt cttcaatttc
     5701 gcgcgcttcg tcaccgtcct cgtcaacggc gccgtcttcg ccgagggcga cgtcgcctcg
     5761 atttccgccg atccgcgggt caaggccgtc tatctcggcg aagacgaggc ccgcgcggga
     5821 gctccgggtg gctgagcttc ttcgcgtcga ggcgctgcgt gccggctacg gcgaggcggt
     5881 cgtcgtccag ggcgtcgatc tcgttctcgc cgaaggcgaa tcgatggccc tgctcggccg
     5941 caacggcacc ggcaagacga cactcctcaa cacgctggtc ggcgtgacgc gccggcacgg
     6001 cggccgcatc gccctcggcg gccgcgacgt cacgaccttg gcgccgcacg cacgcgccgc
     6061 cgccgggatc ggctgggtgc cgcaggaaag aaacatcttt cgctcgctga gcgtcgacga
     6121 gaacctcacc gccgtggccc gtccggggcc atggacgccg gcacgcgtct acgcgatgtt
     6181 tcctcgcctc gccgagcgcc gcgccaacat gggcagccag ctctcgggcg gcgagcagca
     6241 gatgctggcg gtggcccgcg ccctggtcct caatccgaaa ctgctccttc tcgacgagcc
     6301 gctcgagggc ctcgcgccga tcatcgtcaa ggagttgctg gcgtcgatcg cgacggtggt
     6361 tcgcgacgaa ggcgtctcgg cgatcgtcgt cgaacagaat ccgcgacaga tcctgccgat
     6421 cacgagccgc gccgtcattc tcgaccgcgg cgcgatcgtc gacgaaggaa gcagcgccga
     6481 attgctcgcc gatcgcgagc ggctcgaccg ctggctggcg gtggccaggg cctgagcagc
     6541 tcgctgcggc cttcaggtct cgagcggcag gccgacgtag ttctcggcca gcgacgtcga
     6601 ggcagccgcg gagtgaa
//
LOCUS       DASRWS010000596         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2827665, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000596 DASRWS010000000
VERSION     DASRWS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827665"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFF72_11395"
     CDS             complement(<1..908)
                     /locus_tag="VFF72_11395"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HZV93814.1"
                     /translation="MNTAPPFIAPARFDDAAEALARVREIYDASIDHLRTSLQAFVSG
                     AVSPAHVRACYPFVRVHTNTVARADSRLSYGFVAGPGTYETTLTRPDLYSRYYLEQFR
                     LLLENHGIALEIGTSVQPIPVHFSFAEHDHIEGSLSPERRLLLRDLFDLPDLGAMDDG
                     IANGTYEASSGEAQPLALFTAPRVDYSLHRLRHYTGTDPEHFQNFVLFTNYQFYIDEF
                     VRLGHASMAGDATGQGDYEAFVQPGNVIERRRGAPAQPGDELGTAPPRLPQMPAYHLM
                     RPDRSGITMVNIGVGPSNAKTITDHIA"
     gene            1112..2365
                     /locus_tag="VFF72_11400"
     CDS             1112..2365
                     /locus_tag="VFF72_11400"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HZV93815.1"
                     /translation="MKVLVLGAGVIGVTSAYELALAGHQVTVVDRQAAAGLETSFANA
                     GEVSPGYSAPWAGPGVPLKAIKWLAMHHRPLVIRPHIDGPLLGWIVSMLANCTAARYA
                     INKTRMVRLAEYSRDCLRTLRAQTGIAYDERMQGTLQLFRTQRQFDGAANDIEVLRRS
                     GVAFVLLDRDGCVRHEPALANVRDKFVGGLLLPGDETGDCFKFTRALASMAVARGVEF
                     RFTTEVRSLLDAGDRIDAVATNAGTLEADAYLVALGSYSPLLLRPIGLRLPIYPVKGY
                     SITLPVIDDAGAPQSTVMDETHKVAVTRLGGRIRVGGTAELAGYTLKLHEARRKTLLH
                     VVEDLFPKGGDFSAPEFWCGLRPMTPDGTPVIGATPLRNLYLATGHGTLGWTMAAGTG
                     RVLADVISGRSAGIDLSGLTIDRYR"
BASE COUNT          398 a          779 c          889 g          418 t
ORIGIN      
        1 gcgatgtggt cggtgatggt cttggcgttg gacggtccga cgccgatgtt gaccatcgtg
       61 atgccgctgc gatccggccg catgagatgg taggccggca tttgcggcag ccgtggcggc
      121 gccgtgccga gctcgtcgcc cggctgcgcg ggcgcgccgc gccggcgctc gatgacattg
      181 cccggctgca cgaaggcttc atagtctcct tgtccggtcg cgtcgccggc catcgacgca
      241 tgaccgagcc ggacgaactc gtcgatgtag aactggtaat tggtgaagag gacgaagttc
      301 tggaagtgtt ccgggtcggt tcccgtgtag tggcgcaggc ggtgcagcga atagtcgacc
      361 cgtggtgccg tgaagagcgc caagggctgt gcttctccgg aagaagcctc gtaggtgccg
      421 ttggcgatgc cgtcgtccat cgcacccagg tccggcaggt cgaacaggtc gcgcaacagc
      481 aaccgacgct cgggggacag gctgccctcg atatggtcgt gttcggcgaa cgagaagtgg
      541 acgggaatcg gctgcacgct ggtgccgatc tcgagagcga tgccatggtt ttccaggagc
      601 agtcgaaact gttcgaggta gtagcgcgaa tacagatccg gccgggtcag cgttgtttcg
      661 taggtgcccg gcccggcgac gaagccgtaa ctcagccgcg agtcggcacg cgccaccgta
      721 ttcgtgtgga cgcgaacgaa gggatagcag gcccgaacat gtgcgggcga caccgcgccc
      781 gacacgaacg cctgcagcga cgtgcgcagg tgatcgatgc ttgcgtcgta gatttcccgg
      841 acccgggcga gggcctcggc ggcgtcatcg aaacgggccg gagcgatgaa aggaggagct
      901 gtgttcacgt gactcggtcg atgaagcgtg cctaaggtga cgggaatgcg tggcggcggt
      961 tgaggaaggc cacgtgcaga atcgcggcga tcatgcatga tgccccacct cgacatggca
     1021 cggcgctcgt ccgtcccgac gcgtgcgctg cgcccgggcg atcggcgatc gcgtcgcacc
     1081 ggctgcgtca ccggacgacg agacggtggc gatgaaggtc ctcgttctcg gcgccggcgt
     1141 gatcggcgtg acctcggcct acgagctggc gctcgcgggc caccaggtca ccgtcgtcga
     1201 ccgacaggcg gcggccggtc tggaaaccag ctttgccaac gccggcgaag tctcgcccgg
     1261 ctattccgcg ccgtgggccg gtcccggcgt gccgctgaag gcgatcaagt ggctggcgat
     1321 gcatcaccgt ccgctggtga tccgtccgca catcgacgga ccgctcctcg gctggatcgt
     1381 ctcgatgctg gccaactgca ccgcggcgcg ctatgcgatc aacaagacgc gcatggtcag
     1441 gctggccgaa tacagtcgcg actgcctgcg caccctgcgc gcccagaccg gcatcgccta
     1501 tgacgagcgc atgcagggca cgttgcagct tttcaggacg caacggcaat tcgatggcgc
     1561 tgccaacgat atcgaggtgc tccgccgaag cggcgtcgcc ttcgtgcttc tcgaccgtga
     1621 tggatgcgtt cgtcacgagc cggcgctcgc gaacgtgcgc gacaagttcg tcggcggcct
     1681 gcttctgccg ggagacgaaa cgggcgactg cttcaagttc acgcgggccc tggcctcgat
     1741 ggcggtggca cggggtgtcg aattccgatt caccaccgag gttcgcagcc ttctcgacgc
     1801 gggcgaccgg atcgacgccg tggcgacgaa cgcggggacg ctcgaggccg atgcgtacct
     1861 cgtcgcgttg ggaagctact cgccgctgtt gctgcggccg atcggcctgc gcctgcccat
     1921 ctaccccgtc aagggctact cgatcacgct gccggtgatc gacgatgcgg gtgcgccgca
     1981 gtcgacggtg atggatgaaa cgcacaaggt cgcggtgacg cgtttgggcg gtcgcatccg
     2041 cgtcggcggc acggccgagc ttgcgggcta cacgctgaag ctccacgagg cccggcgcaa
     2101 gacccttttg cacgtcgtcg aggacctgtt ccccaagggg ggcgacttct cggcgcctga
     2161 gttctggtgt ggcctgcggc ccatgacgcc ggatggcacg ccggtgatcg gcgcgacgcc
     2221 gctgcgcaat ctctacctcg ccaccggcca cggcaccctg ggctggacga tggcggcggg
     2281 gacgggcagg gtgctggccg acgtgatttc gggacgcagc gctggcatcg acctcagcgg
     2341 actgacgatc gatcgctatc ggtgacgggc atcggcccgc tccggacgcg gccggtgcag
     2401 cgtcacccct tgggagtcag agcgattcgg cgaaggcact ggcaggggcg tccggccagt
     2461 gcctcgcctg cagggagggg tgcg
//
LOCUS       DASRWS010000597         6174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1386588, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000597 DASRWS010000000
VERSION     DASRWS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386588"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VFF72_11405"
     CDS             <1..899
                     /locus_tag="VFF72_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZV93816.1"
                     /translation="PVFMMKALCYAMFACAFNLLLGFTGLLSFGHAAYLGIAAYATGW
                     LVRTAGWSPEFGVLAGTAAAAALGLVVGLIAIRREGIYFAMITLALAQMVYFVFLQAP
                     FTGGEDGLQGVPRGRLFGLLSLDNDLVLYYLVLAVFVAVFLFVIRVVHSPYGQVLKAI
                     RENEPRAISLGYDVARYKLLAFVLSTALAGLAGSMKTLVLGFASLSDAHWTMSGEVIL
                     MTLLGGMGTFAGPVVGAFTIVGLQNFLSDRVGSWINVIIGAIFVVCVVAFRRGFIGEL
                     QAWLRRRSNYTVDHSVGKDPGP"
     gene            896..1303
                     /locus_tag="VFF72_11410"
     CDS             896..1303
                     /locus_tag="VFF72_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93817.1"
                     /translation="MSKRLTKGELDEMRTLGALNPVNYVVLALASDAVAADAKAALGA
                     AGFSDEDILAYSSRELFPDLEAMLRKASGAAGFGYEITLMRRYMTLASEGCGWLMVYS
                     PDEAQTAKVQEVAKRFNARSAVHYGRLAHQDLV"
     gene            complement(1310..2320)
                     /locus_tag="VFF72_11415"
     CDS             complement(1310..2320)
                     /locus_tag="VFF72_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZV93818.1"
                     /translation="MTPGGRGFRRLAAVAAGLLIVASGQTVRAVAPTFRIEVHSLATQ
                     TPTTTQLLTGTGGNAPPATIAGELSLPFAAADRLPAVIFLHGDAGIIADQVAWIDELN
                     AMGIAVFTLDSFSGRGAISPGASIASMPDSVGGTARVVDAERALAMLARHPRIDPQRI
                     AVMGVSSGGRTVITAAMSRFAGAWGTPGLAFSAYIALYPPCNVQLIDDTKMEPGPLRI
                     FHGEADVVTSAEACSRYVDRLRRAGNDAAIRTFPGAHHGFDNVANPGLIRLPDAPNGA
                     RCRVEERTIGVVLNADTGRPIGLGDACFGKGIVAGYDAAADAATKQAVKELLAERFGL
                     KR"
     gene            complement(2322..3395)
                     /gene="ruvB"
                     /locus_tag="VFF72_11420"
     CDS             complement(2322..3395)
                     /gene="ruvB"
                     /locus_tag="VFF72_11420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZV93819.1"
                     /translation="MSIQTDDFAGHPAPANARVVSAAAGSPNEEAIERALRPKGLAEY
                     VGQAKAREQLEIFIGAAKKRGEAMDHVLLFGPPGLGKTTLSHIIAHELGVSLRQTSGP
                     VLEKPKDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARS
                     IKLELQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYSADELATIVRRSAGLLKVALD
                     DAGGFEIARRSRGTPRIANRLLRRVRDYADMKGSGQITKPLADKALVMLDVDPHGLDV
                     MDRKLLEAVIRRFDGGPVGLDNVAAAIGEERDTIEDVIEPFLIQQGFLQRTPRGRIAT
                     LAAYRHLGVAPPKSTGGLFAEGA"
     gene            3428..3976
                     /locus_tag="VFF72_11425"
     CDS             3428..3976
                     /locus_tag="VFF72_11425"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZV93820.1"
                     /translation="MHHPSTLRRATALVLAALVVSSLGALAGAPVSVLAAASTGIRVE
                     HPFATPSVPGATMGAAYFGVLENHGAKPDKLVRATTPVAASVEMHTMAVDPHGVMRMR
                     EVDGIAIAPGGVVRMRPGMGYHLMLVGLKQPLKPGESFPMSLQFEHAGNVDVTVVVQA
                     MGGAAPMSGMLGMPGMSGMPKH"
     gene            complement(4026..4601)
                     /gene="ruvA"
                     /locus_tag="VFF72_11430"
     CDS             complement(4026..4601)
                     /gene="ruvA"
                     /locus_tag="VFF72_11430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZV93821.1"
                     /translation="MIGRLTGTIAEKSPPQLLVDVRGVAYEVDVPMSSFYNLPAIGET
                     VTLLTHFVVREDAQILFGFLTHQERATFRLLVRISGVGPRTALAILSGLSVEELARAV
                     SLQESGRLVKVPGIGKKTAERLLLELKGKLADAVAVPTSVASSAHGDVLQALVALGYS
                     DREAAAAAKTLPADLDDVAAGIKAALRALAK"
     gene            4778..5746
                     /locus_tag="VFF72_11435"
     CDS             4778..5746
                     /locus_tag="VFF72_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZV93822.1"
                     /translation="MILRHAFIPPDNARLANLAGSLDEHLRTIAAAFEVKIHRRNESF
                     RVEGAKGRAERAVGLLQHLYGRAARPIAAAEVELAILEAMSADEGGGEAPPGADAQID
                     LRLRTRRGDLAGRTPNQVAYLRNILAHDLTFGIGPAGTGKTYLAVACAVDALERNAVQ
                     RIILTRPAVEAGERLGFLPGDLAQKVYPYLRPLYDALYELMGLDRVGKAFEKATLEIA
                     PLAFMRGRTLNHAFVILDEAQNTTTEQMKMFLTRIGFGSKAVVTGDISQVDLPKGTVS
                     GLIEAEQVLQGVAGIATTHFTSADVVRHPLVARIVEAYGRADADRS"
     gene            5748..>6174
                     /gene="ybeY"
                     /locus_tag="VFF72_11440"
     CDS             5748..>6174
                     /gene="ybeY"
                     /locus_tag="VFF72_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZV93823.1"
                     /translation="MALDREALTLSLQFADAAHRAVLARHRVGRWLRAALESPAEIAV
                     RIVGAAEGLRLNREFRGQGHATNVLTFDYERSPRVVADLVLCAPVVEREAKAMGVPIE
                     AHYAHLLVHGALHAQGLDHQRSDEARAMEARESAIVISLG"
BASE COUNT          881 a         2105 c         2186 g         1002 t
ORIGIN      
        1 atccggtctt catgatgaag gccctttgct atgcgatgtt cgcctgcgcc ttcaacctgc
       61 tgctcggctt caccggcctg ctgtcgttcg gccacgccgc ctatctcggc atcgccgcct
      121 acgccaccgg ctggctggtg cgaacggcgg gctggtcgcc cgagttcggc gtgcttgccg
      181 gaacggcggc cgcggccgcg ctcggcctcg tcgtcggcct gatcgcgatc cgtcgcgagg
      241 gcatctactt cgcgatgatc acgctggcgc tggcgcagat ggtctacttc gtcttcctcc
      301 aggcgccctt caccgggggc gaggacgggc tgcagggcgt gccgcgcggc aggctcttcg
      361 gcctcttgtc cctggacaac gacctcgtcc tctactacct tgtcctggcg gtcttcgtcg
      421 ccgtctttct cttcgtcatc cgtgtcgtcc attcgcccta cgggcaggtg ctgaaggcga
      481 tccgcgagaa cgagccgcgg gcgatctcgc tcggctacga cgttgctcgc tacaagctgc
      541 tcgccttcgt gctgtcgacg gcgctcgccg gcctggccgg ctcgatgaag acgctggtgc
      601 tcggcttcgc ctcgctgtcc gacgcccact ggacgatgtc gggcgaggtc atcctgatga
      661 cgctgctcgg cggaatgggc acgttcgccg ggccggtggt cggcgccttc acgatcgtcg
      721 gcctgcagaa cttcctgtcc gaccgggtcg gctcgtggat caacgtcatc atcggcgcga
      781 tcttcgtcgt ctgcgtcgtc gccttccggc gcggattcat cggcgagttg caggcgtggc
      841 tgcggcgccg gtcaaattac actgtggatc attctgtcgg caaggatccc gggccatgag
      901 caagcggttg acgaaaggcg agctggacga gatgcgcacg ctgggtgcgc tcaacccggt
      961 gaattacgtc gtgctggcgc ttgcgagcga cgccgtcgcg gccgacgcga aggcggcgct
     1021 cggcgcggcg ggcttcagcg acgaagacat cctcgcctac tcctccaggg agctcttccc
     1081 cgacctcgag gcgatgctgc gcaaggcgag cggcgcggcc ggcttcggct acgagatcac
     1141 cttgatgcgc cgctacatga cgctggcgag cgagggttgc ggctggctga tggtgtattc
     1201 gcccgacgaa gcccagaccg cgaaggtcca ggaagtcgcg aagcgattca acgcccgctc
     1261 ggcggtgcac tacggccgcc tggcccatca agacctggtc tgacggcttt cagcgcttca
     1321 ggccgaagcg ttcggcgagc agttccttga cggcctgctt ggtggcggca tcggcggcgg
     1381 cgtcgtagcc ggcgacgatc cccttgccga agcaggcgtc gccgagccct atcggccggc
     1441 ccgtgtcggc attcagcacg acgccgatcg tcctttcctc cacccggcag cgcgctccgt
     1501 tcggtgcgtc cgggaggcgg atcagaccgg ggttggcgac gttgtcgaaa ccgtgatggg
     1561 cgcccgggaa ggtgcggatc gccgcgtcgt tgccagcgcg acgaaggcga tcgacgtagc
     1621 gcgagcacgc ctcggccgag gtgacgacgt cggcctcgcc gtggaagatg cgcagcggcc
     1681 ccggttccat cttcgtgtcg tcgatcagct gcacgttgca cggcgggtag agcgcgatgt
     1741 aggccgaaaa cgccaggcct ggcgtgcccc aggccccggc gaagcgcgac atggcggcgg
     1801 tgatcacggt ccggccgccc gacgagacgc ccatcacggc gatgcgttgc gggtcgatgc
     1861 gcggatgccg ggccagcatc gcgagcgcgc gctcggcgtc gacgacgcgg gccgtgccgc
     1921 ccaccgagtc cggcatcgag gcgatgctcg cgcccgggct gatggcgccg cggccgctga
     1981 agctgtcgag cgtgaagacg gcgatgccca tggcattgag ctcgtcgatc caggcgacct
     2041 ggtcggcgat gatgccggcg tcgccatgca ggaagatcac cgcgggcagg cggtcagcgg
     2101 cggcgaaggg caggctcagc tcgccggcga tcgtggcggg cggcgcgttt cctccggttc
     2161 ctgtcagcaa ctgggtggtc gtcggcgtct gcgtggccag cgaatgcacc tcgatgcgga
     2221 aggtgggggc gacggcccgg acggtttgcc ccgacgcgac gatcaggagg ccggcggcca
     2281 ccgcggcgag gcgcctgaag ccgcgaccac cgggcgtcat gtcatgcgcc ctcggcgaac
     2341 agcccgcccg tgctcttcgg cggcgcgacg ccgaggtgcc gataggccgc cagcgtggcg
     2401 atccggccgc gcggcgtgcg ctgcaggaag ccctgctgga tgaggaaggg ctcgatcacg
     2461 tcctcgatcg tgtcgcgctc ctcgccgatc gcggcggcca cgttgtcgag gccgaccggg
     2521 ccgccgtcga agcggcggat caccgcctcg aggagctttc ggtccatcac gtcgaggccg
     2581 tgcgggtcga cgtcgagcat gaccagcgcc ttgtcggcga gcggcttggt gatctggccg
     2641 ctgcctttca tgtcggcgta gtcgcgcacg cggcgcagca gccggttggc gatgcgtggg
     2701 gtgccgcgcg agcgccgtgc gatctcgaag ccgccggcgt cgtcgagcgc caccttgagc
     2761 aggcccgccg agcggcgaac gatcgtcgcc agctcgtcgg ccgagtagaa ctcgagccgg
     2821 gcgacgatgc cgaagcggtc gcgcaatgga ttggtgagca tgccggcgcg ggtcgtcgcg
     2881 ccgaccagcg tgaacggctg cagctcgagc ttgatcgagc gtgccgccgg gccctcgccg
     2941 atcatgatgt cgatctggta gtcctcgagc gccgggtaga ggatctcctc gacgaccggc
     3001 gacaggcgat ggatctcgtc gatgaagagg acatcgttct tctcgaggtt ggtgaggagg
     3061 gcggcgaggt ccttgggctt ttcgagcacc gggcccgagg tctggcgcag gctgacgccg
     3121 agctcgtggg cgatgatgtg cgagagcgtc gtcttgccga ggccgggcgg gccgaagagc
     3181 agcacgtggt ccatcgcctc gccgcgcttt ttcgccgcgc cgatgaagat ctcgagctgt
     3241 tcgcgggcct tggcctggcc cacgtactcc gccaggccct tgggccgcag cgcccgctcg
     3301 atcgcttcct cgttcggcga gccagcggcc gcgctgacga cgcgcgcgtt ggccggcgcc
     3361 gggtggccag cgaagtcgtc ggtctggatg ctcatgcggg cattatcggc agcgtagact
     3421 gacgcgcatg catcacccat cgactcttcg ccgtgccacc gcgctggtgc tggcggctct
     3481 cgtcgtcagc agcctcggtg cgctcgcggg cgcgccggtt tccgtgctcg ccgccgcttc
     3541 caccgggatc cgggtcgagc atcctttcgc gactcctagc gtgcccggcg cgacgatggg
     3601 cgcggcctat ttcggtgtgc tcgagaacca tggcgccaaa cccgacaagc tggtcagggc
     3661 gacgacgccg gtggctgcca gcgtcgagat gcacaccatg gcggtcgacc cgcacggcgt
     3721 gatgcggatg cgcgaggtcg acggcatcgc cattgccccc ggcggcgtcg tgagaatgcg
     3781 gccgggcatg ggctatcacc tcatgctcgt cggcctgaag cagcccctca agccgggcga
     3841 gtcgttcccg atgtcgctgc agttcgagca tgccggcaac gtcgacgtca cggtggtcgt
     3901 gcaggcgatg ggaggggcag cgccgatgtc gggcatgttg gggatgcccg gcatgtcggg
     3961 catgccgaag cactgaaggt cggccgatcc ggcccggccg gcgacagcgg cgcgcgcgcc
     4021 gcgacctact tcgccaacgc acgcaacgcc gccttgatcc cggcggcgac gtcgtcgaga
     4081 tcggcaggca gcgtcttcgc tgccgccgcc gcttcgcgat cgctgtagcc gagggcgacc
     4141 agcgcctgca ggacgtcgcc gtgcgcgctc gaggcgacgc tggtcggtac cgccacggcg
     4201 tcggcaagct tgcccttcag ctcgagcagc agcctctcgg ccgtcttctt gccgatgccc
     4261 ggcaccttga cgagacgccc gctctcctgg aggctcaccg cgcgcgccag ctcctcgacc
     4321 gacagacccg agaggatcgc gagcgccgtg cgcggcccga caccggagat cctgacgagc
     4381 agccggaagg tggcgcgctc ctggtgcgtc aggaagccga acaggatctg cgcatcctcg
     4441 cggacgacga agtgggtgag caacgtcacc gtctcgccga tcgccggcag gttgtagaag
     4501 ctcgacatcg gcacgtcgac ctcgtaggcg acgccgcgca cgtcgacgag caactgcggc
     4561 ggcgacttct cggcgatggt tccggtgagg cggccgatca tgggccgcga ttatccggcg
     4621 ccatcgtcga gcgcgcgcgg agtcattcgg gacgttcgct cgacattgcc acgaccggca
     4681 cgcgttcgtg ccgcatgcgc cggatcggac cccagcacgg ccgacgcggc aagcggtggc
     4741 acgctgcata cactacctgc gcccgggagc atctcacttg atccttcgcc acgccttcat
     4801 accgcccgac aacgcccgac tggccaacct cgccggcagc ctcgacgagc acctgcgcac
     4861 gatcgccgcc gccttcgagg tgaagatcca ccggcgcaac gaatcatttc gcgtcgaagg
     4921 ggccaaggga cgtgccgagc gcgcggtggg cctgttgcag cacctgtacg gccgggcggc
     4981 ccggccgatc gccgccgccg aggtcgagct ggccatcctc gaggcgatgt cggccgacga
     5041 gggtggcggc gaggcgccgc cgggagcaga tgcgcagatc gacctgcggc tgcgaacgcg
     5101 ccgcggcgat ctggccggcc ggacgccgaa ccaggtcgcc tacctgcgca acatcctcgc
     5161 ccacgatctc accttcggca tcggacctgc gggaaccggc aagacctatc tcgcggtggc
     5221 ctgcgcggtc gatgcgctcg agcgaaacgc cgtgcagcgg atcatcctca cgcggcccgc
     5281 cgtcgaggcc ggcgagcggc tcggctttct tcccggcgac ctcgcgcaga aggtctatcc
     5341 ctacctgcgt ccgctctacg acgcgctcta cgagctgatg ggcctggacc gtgtcggcaa
     5401 ggcgttcgag aaggcgacgc tcgagatcgc gccgctcgcc ttcatgcgtg gccggaccct
     5461 caatcatgcg ttcgtgatcc tcgacgaagc gcagaacacg acgaccgagc agatgaagat
     5521 gttcctgacg cgcatcggct tcggctcgaa ggccgtcgtc accggcgaca tcagccaggt
     5581 cgacctgccc aagggaacgg tctcgggact gatcgaggcc gagcaggtgc tgcagggcgt
     5641 ggccggaatc gcgacgacgc atttcacgtc ggccgatgtc gtgcgccatc cgctggtcgc
     5701 ccgaatcgtc gaggcgtacg gccgcgccga cgccgacagg tcctgaggtg gcgctggacc
     5761 gcgaggcgct gacgctgtcg ctgcagttcg ccgatgccgc gcatcgggcg gtgctggcgc
     5821 ggcatcgcgt cggccgctgg ctgcgtgcgg cgctcgaatc ccccgccgag atcgcggtgc
     5881 gcatcgtcgg tgccgccgaa ggcctgcgcc tgaaccgcga attccggggc cagggtcacg
     5941 ccaccaacgt cctcaccttc gactacgagc gatcgccgcg cgtcgtcgcc gatctggtgc
     6001 tgtgcgcgcc cgtcgtcgag cgagaggcga aggctatggg cgtgccgatc gaggcgcact
     6061 acgcgcatct gctggtgcat ggcgcgctgc acgcgcaggg gctcgatcac cagcgcagcg
     6121 acgaggcgcg ggcgatggag gcgcgcgagt cggcgatcgt catctcgctc ggct
//
LOCUS       DASRWS010000598         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3973933, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000598 DASRWS010000000
VERSION     DASRWS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3973933"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFF72_11445"
     CDS             <1..1152
                     /locus_tag="VFF72_11445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZV93824.1"
                     /translation="ADVTKRRDKGAVHGPRILVLTPTRELAIQIAKAASTYGRHVAGL
                     RVATVVGGVPYGAQLKALRGPLDILIATPGRLLDHLQTGKAVIGNVEMFVLDEADRML
                     DMGFIDDIRTIADHLPRPRQTVMYSATFLGNVAGLARSLLENPQRVDVASHTDTHANI
                     EQRLHWADNGAHKNALLDHILTQREIDQALVFTSTQRDADWLADRLADLGHSVAALHG
                     GMPQGRRNRVLQGLRSRQLRILVATDVAARGIDVRTISHVVNYGLPMKPEDYVHRIGR
                     TGRAGKNGLAVTLAERGDSGMIRRIQQFTTQAIPVATIGGLEPKGLPPRAAKPSSVAA
                     GRPGTRSNPFDRALPSRDDRRPAPRRPGPGAFKGRSVSSRASRPGGFAR"
     gene            complement(1255..2136)
                     /locus_tag="VFF72_11450"
     CDS             complement(1255..2136)
                     /locus_tag="VFF72_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZV93825.1"
                     /translation="MPIRYEIVHTTLYSYRQPVAFGEHRVMFRPRDSHDLRVLATDLE
                     VSPGADIRMIQDPLSNSVALVRPRVACASLRIVCSFTIEHAHSLNLELPLSPGAELFP
                     FSYTLEERLDLEHYLRPHHEDPGGHLAAWARQFIRTDGPTRTRDLLVHMNQHIRNNFR
                     YGSRDEEGTQSPAETLTLGSGSCRDFALLMMEAARRLGIASRFVSGYLYDPSLEGGEG
                     EAVVGAGYTHAWLQVYLPGAGWVPFDPTNNLLGGTQLIRVAIARDPAQAAPISGSWYG
                     QADDYLGMNVNVAVRRA"
     gene            complement(2152..2499)
                     /locus_tag="VFF72_11455"
     CDS             complement(2152..2499)
                     /locus_tag="VFF72_11455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZV93826.1"
                     /translation="MQTTDSGLQYEDTVPGSGKQAEAGTPVTVHYTGWLYDAGEKKAK
                     FDSSKDRGDPFAFDLGAGHVIKGWDEGVQGMKEGGTRILVIPPHLGYGARGAGGVIPP
                     DATLLFEVELLRA"
     gene            complement(2555..3451)
                     /gene="gluQRS"
                     /locus_tag="VFF72_11460"
     CDS             complement(2555..3451)
                     /gene="gluQRS"
                     /locus_tag="VFF72_11460"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZV93827.1"
                     /translation="MTPRYTGRFAPSPTGLLHAGSLVAALASWLDARANRGRWLIRIE
                     DLDTSRNQPGAAEAIVGQLARCGLRADEPALRQSGRGAAYQAALDRLLDAGAAYPCGC
                     SRRDIETMAVATAVDDASGPIYPGTCRDGLHGRPARSFRLRTAGDSAVPLPSAIVEWT
                     DRRLGRQREDVARSVGDFVLRRADAVWAYQLAVVVDDAAQGVTDVVRGEDLAGSTARQ
                     IFLQRALGLPTPRWLHVPLILAADGQKLSKQTGARPLDLEDPVKALRAAGAVLDVTLD
                     GSTVGEALANGISAWRRRWPLP"
     gene            complement(3473..>3754)
                     /locus_tag="VFF72_11465"
     CDS             complement(3473..>3754)
                     /locus_tag="VFF72_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93828.1"
                     /translation="LGCGFVPEPMARDHIAGGRLVVKQVERPRFDARLGYAWRTPVGA
                     RGGRKTPPGLALGWWLEQLASAATRRSLLERHGGRVGNRSLVSAADDRS"
BASE COUNT          575 a         1352 c         1293 g          534 t
ORIGIN      
        1 gccgacgtca cgaagcgacg cgacaagggc gccgtgcacg gcccccgcat cctcgtgctg
       61 acgccgaccc gcgagctggc gatccagatc gccaaggcgg catcgactta cggccggcac
      121 gtcgccggcc tgcgcgtggc taccgtcgtc ggcggcgttc cctacggcgc gcagctgaag
      181 gccctgcgcg gcccgctcga catcctgatc gcgacaccgg gtcgcctgct cgatcacctg
      241 cagacgggca aggcggtgat cggcaacgtc gagatgttcg tgctcgacga agccgatcgc
      301 atgctcgaca tgggcttcat cgacgacatc cgcaccatcg ccgaccacct gccacggccg
      361 cgccagacgg tgatgtacag cgccacgttt ctcggcaacg tcgccggcct cgcgcgaagc
      421 ctgctcgaga acccccaacg cgtcgacgtc gcctcgcaca ccgacaccca cgccaacatc
      481 gagcaacggc tgcactgggc cgacaacggc gcgcacaaga acgcgctgct cgaccacatc
      541 ctgacccagc gcgagatcga ccaggcgctc gtcttcacga gcacgcagcg cgatgccgac
      601 tggctcgccg atcgtctcgc cgacctcggt cattcggtcg ccgcattgca cggcggcatg
      661 ccgcagggcc gtcgcaatcg cgtcctgcag ggcctgcgtt cgcggcagtt gcgcatcctg
      721 gtcgccaccg acgtggccgc gcgcggcatc gacgtgcgca ccatcagtca cgtcgtcaac
      781 tacggcctgc ctatgaagcc ggaagactac gtgcaccgga tcggccgcac cggtcgtgcc
      841 ggcaagaacg gcctcgccgt gacgctggcc gagcgcggcg acagcggcat gatccggcgc
      901 atccagcaat tcacgacgca ggcgattccg gtggcgacca tcggcggcct cgaaccgaag
      961 ggcctgcccc cgagggcggc gaagccgtcg tcagtcgcgg ccggtcggcc ggggactcgt
     1021 tccaaccctt tcgatcgggc gctgccgagt cgcgacgacc gccgccccgc gccgcgccgg
     1081 cctggaccgg gtgcgttcaa gggccgctcg gtctcgtcgc gcgcttctcg accgggcggc
     1141 ttcgcgcgct gagcaaaggc gcatcgttcg gatcgagacc ggcctgcgcg cgacgagcgc
     1201 gacagactgg cggcgcgcgg agtgtcgccg ccttgggccc ggtgatcgac cggatcaggc
     1261 tcgacgcacc gcgacgttga cgttcatccc gaggtagtca tcggcctggc cgtaccagct
     1321 gcccgagatc ggcgcggcct gcgcggggtc gcgcgcgatc gcgacgcgga tgagctgcgt
     1381 gccgccgagc aggttgttgg tcggatcgaa cggcacccat ccggccccgg gcaggtagac
     1441 ctgcagccac gcgtgcgtgt agcctgcgcc gaccacggcc tcgccctcgc cgccctcgag
     1501 cgacgggtcg tagagatagc cggagacgaa gcggctggcg atgcccagcc gtcgcgcagc
     1561 ttccatcatc aacagcgcga agtccctgca cgagccgctg cccagcgtca gcgtttccgc
     1621 cggcgactgc gtgccttcct cgtcgcgcga gccgtagcgg aagttgttgc ggatgtgctg
     1681 gttcatgtgg acgagcaggt cgcgcgtccg ggtcgggccg tcggtgcgga tgaactggcg
     1741 cgcccaggcg gcaaggtgcc ctcccggatc ctcgtggtgc ggccgcaggt aatgctcgag
     1801 gtcgagtcgc tcctcgagcg tgtagctgaa cggaaagagc tcggcacccg gcgagagagg
     1861 cagctccagg ttcagcgaat gcgcatgctc gatcgtgaag ctgcagacga tccgcagcga
     1921 cgcgcacgcc acccgaggcc gcaccagcgc gaccgagttc gacagcggat cctggatcat
     1981 gcggatgtcg gccccggggc tcacttcgag gtcagtggcg aggacgcgca ggtcgtggct
     2041 gtcgcgaggc cggaacatga cccggtgctc gccgaaggcg acgggctggc gatagctgta
     2101 cagcgtcgtg tggacgatct cgtagcggat cggcatgacg ctgccccggt ctcaggcgcg
     2161 cagcagttcg acttcgaaca gcagcgtcgc atcgggcgga atgacgccgc cggcgccgcg
     2221 tgcgccgtag cccaggtgcg gtggaatgac gaggatgcgc gtaccgcctt ccttcatgcc
     2281 ctgcacgcct tcgtcccagc ccttgatgac atggccggcg ccgaggtcga acgcgaaggg
     2341 atcgccgcga tccttgctgg aatcgaactt cgccttcttc tcgccggcgt cgtacagcca
     2401 gccggtgtag tgcaccgtca ccggcgtgcc agcctccgcc tgcttgccgc tgccgggcac
     2461 ggtgtcttcg tactgcaagc cggaatcggt cgtctgcatg gagggtcctt tcgagcggcc
     2521 aaaacgacgg atgatgccat ccgtcatcga tgagtcaggg cagcggccaa cggcgccgcc
     2581 acgccgatat cccgttggca agcgcctcgc ccacggtcga tccatcgagc gtcacgtcga
     2641 gcacggcgcc ggcggcgcgc agcgccttca ccggatcctc gagatcgaga ggccgtgcgc
     2701 cggtctgctt ggacagtttc tgcccatcgg cggcgaggat cagcggcacg tgcagccagc
     2761 gcggcgtcgg caagccgagc gcgcgctgca gaaagatctg ccgcgccgtg ctgccggcca
     2821 ggtcttcgcc gcggacgacg tcggtgaccc cttgcgccgc gtcgtcgacg accaccgcga
     2881 gctggtaggc ccacacggca tcggcccgcc tgagcacgaa gtcgccgacc gagcgggcca
     2941 cgtcctctcg ttgccggccg agtcggcgat cggtccattc gacgatcgcc gaaggcagcg
     3001 gcacggccga gtcgcccgcc gtgcgcagcc tgaacgagcg tgccggccgg ccgtgcaggc
     3061 catcccggca ggttcccgga tagatgggac cggaggcgtc gtcgaccgcc gtcgcaacgg
     3121 ccatcgtttc gatgtcgcga cggctgcagc cgcacggata cgcagcgccg gcatcgagca
     3181 gtcgatcgag tgccgcctga taggcggccc cgcggcccga ctggcgaagc gcaggctcgt
     3241 cggcgcgcag gccgcagcgc gccagctgcc cgacgatcgc ctcggccgcg cccggctggt
     3301 tgcgcgacgt gtcgaggtcc tcgatccgga tcagccagcg accgcgattc gcgcgcgcgt
     3361 caagccacga cgccagcgcg gcgaccagcg atcccgcgtg cagcagtccg gtcggcgacg
     3421 gcgcgaagcg tccggtatag cgcggcgtca tcgccgtcct tcctccgaca cgtcaggacc
     3481 gatcgtctgc cgccgagacg aggctgcgat tgccgacccg gccgccgtgt cgctcgagca
     3541 gcgagcgccg cgtcgccgcg ctcgccagct gctcgagcca ccagccgagg gccagccccg
     3601 gtggcgtctt gcgaccgccg cgggcaccga ccggcgtgcg ccaggcgtag cccaggcgcg
     3661 cgtcgaagcg gggccgctcg acctgcttga cgacgagtcg tccgccggca atgtggtcgc
     3721 gggccatcgg ctcgggcacg aagccgcagc cgag
//
LOCUS       DASRWS010000599         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1110504, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000599 DASRWS010000000
VERSION     DASRWS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110504"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFF72_11470"
     CDS             complement(<1..315)
                     /locus_tag="VFF72_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93829.1"
                     /translation="MSLTLYYHPLSSFCWKTLIALYEAGVAFEARQVNLGDPADRAAF
                     AAVWPLAKFPVLRDDARGKTIPESSIIIEYLARTEPAAASLLPRDPDEAMQVRLVDRL
                     IDA"
     gene            422..1639
                     /locus_tag="VFF72_11475"
     CDS             422..1639
                     /locus_tag="VFF72_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HZV93830.1"
                     /translation="MDDFLATLYRQESRRVFSTLLRLLGSFDVAEDALHDAFIAAAEQ
                     WPARGIPAHPVAWLVSAGRHRAIDRLRRERLYVAADDAAAQIEAAIDESPDWAERRDA
                     RDAIEDDRLRLVFTCCHPSLSEEARIALTLREVCGLTTEAIAGAFLIATPTLAQRIVR
                     AKAKITAARIPYEVPGPAERPARLASVLRVVYLVFTEGHAAHAAELADEAIRLARLLQ
                     SLLPEPEVTGLLALLVLHDARRDARTSPSGELVALEDQDRSRWNHAAIAEGCALAHEA
                     LSRRGFGVYALQAAIAAVHAEAPSTPATDWPQIVGLYDALLRAEPSPVIELNRAVAVA
                     MRDGPDAGLALIEPLIPVLAGYHAAPAAAADLCRRAGRLDEAETHYRHALALARQEPE
                     RGFLMRRLADLRI"
     gene            1733..2188
                     /locus_tag="VFF72_11480"
     CDS             1733..2188
                     /locus_tag="VFF72_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV93831.1"
                     /translation="MPTTLTPYLGFDGNTREAFAFYEQALGATIKAMLTYAQMPPQPA
                     PAGSAEGCSGQMPSGNAIMHACLVLPGGAMLFAGDAPPGVPYEGIKGNMLALQYDTVD
                     QAHSAFDALAKGGKVTMPLAPTFWARTFGMLTDRFGVSWAVNGEPIPMK"
     gene            2202..2534
                     /locus_tag="VFF72_11485"
     CDS             2202..2534
                     /locus_tag="VFF72_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZV93832.1"
                     /translation="MKYLCLVYLDAENWAACGDAECADYVQQLADGRRLLAAEPLHRA
                     HTATTVRVRGGEVTLFDGPFAETREMLAGFYLVDARDLNEAIQLAARIPPARYGSVEV
                     RPVRELQP"
     gene            2679..>3142
                     /locus_tag="VFF72_11490"
     CDS             2679..>3142
                     /locus_tag="VFF72_11490"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HZV93833.1"
                     /translation="MLLLAAAALVAVTALLAQPWLVRRRRARLRARAFPAAWRRILRR
                     RVPLVARLPPHLQLRLKGHIQVFIAEKPFIGCNGQRITDDVRVTIAAQACLLLLGHPQ
                     PDCFPRLRQVLVYPDAFVADRERPVGAGLVQAQRHTLAGESWTQGQVILSWA"
BASE COUNT          471 a         1138 c         1051 g          482 t
ORIGIN      
        1 tgcgtcgatg aggcgatcga cgaggcggac ctgcatcgcc tcgtccggat cgcgcggcag
       61 cagcgatgcc gcggcgggct cggttcgcgc caggtactcg atgatgatgc tcgactccgg
      121 gatcgtcttg cctcgcgcgt cgtcgcgcag caccgggaac ttggccagcg gccagaccgc
      181 cgcaaaggcg gctctgtcgg ccgggtcgcc gagattgacc tgccgcgcct cgaaggcgac
      241 gccggcctca taaagcgcga tcagcgtctt ccagcagaag gaggaaagcg ggtggtagta
      301 aagggtcagg gacatccggc actctgatac ttaagtaatg aattaagtat agagagctcc
      361 ggtcacgacg tcaagcagtc ctcctgattc gcaggtcggg cgtgggctta cccttcgcgc
      421 catggacgac ttcctggcca ccctctaccg gcaagagagc cggcgggtgt tctcgacact
      481 gctgcgactg ctcggcagct tcgacgtcgc cgaggacgcg ctgcacgatg ccttcatcgc
      541 cgccgcggag cagtggccgg cgcgcggcat tccggcccat cccgtggcct ggctggtttc
      601 ggccggacgc caccgggcga tcgatcggct gcgtcgggaa cgactctacg tcgccgcgga
      661 cgatgccgcc gcgcagatcg aggcggcgat cgacgaatct cccgactggg cagaacgccg
      721 cgacgcccgc gacgcgatcg aggacgaccg cctgcgcctc gtcttcacct gctgtcaccc
      781 gagcctctcg gaagaggcgc gcatcgccct cacgttgcgc gaggtgtgcg gtctcacgac
      841 cgaggcgatc gccggcgcgt tcctgatcgc cacgccgacg ctggcccagc gcatcgtccg
      901 cgccaaggcg aagatcaccg cggcccgcat tccgtacgaa gtgccgggcc ccgcggagcg
      961 gccggcgcgg ctcgcgagcg tgctgcgcgt cgtctacctc gtcttcaccg agggccatgc
     1021 ggcgcacgcc gccgaactgg ccgacgaggc catccggctg gcgcgcctcc tgcagtcgct
     1081 gctgcccgag ccggaggtca ccggcctgct cgcgctgctg gtgctgcacg atgcgcgtcg
     1141 cgacgcacgc acctcgccga gcggagagct cgtggccctc gaggaccagg accggtcgcg
     1201 ctggaaccac gccgccatcg ccgaaggctg cgcgctggcg cacgaggcgc tgtcgcgccg
     1261 gggcttcggc gtctacgcgc tgcaggcggc gatcgccgcc gtgcacgccg aggcgccgag
     1321 cacgcctgcc accgactggc cgcagatcgt cggcctctac gacgccctcc tgcgcgccga
     1381 gcccagcccg gtcatcgagt tgaaccgcgc cgtcgccgtg gcgatgcgcg acggccccga
     1441 cgccgggctc gccctgatcg agccgctgat ccccgtcctt gccggctacc acgccgctcc
     1501 ggccgccgct gccgatcttt gccgacgagc cgggcggctc gacgaggccg agacccacta
     1561 ccgtcacgcc ctggcgctgg cacgacagga gccggagcgc ggcttcctga tgcgccggct
     1621 ggccgatctg cgcatctgaa gaaaaaattt ccccgacgct gtcgaaagcc ggaccgctcg
     1681 ttcgactacc tgatgcgccc tttgcgacaa cccatcaccc ccaggagcac ccatgcccac
     1741 caccctcacg ccttacctcg gcttcgacgg caacacccgc gaggctttcg ccttctacga
     1801 gcaggcgctc ggcgccacga tcaaggccat gttgacgtac gcccagatgc cgccgcagcc
     1861 cgcacccgcc ggcagcgccg agggctgcag cggccagatg ccgagcggca acgccatcat
     1921 gcatgcgtgc ctggtgctgc cgggtggcgc gatgctgttc gcgggcgatg cgcctccggg
     1981 cgtgccttac gaaggcatca agggcaacat gctcgccctc cagtacgaca cggtcgacca
     2041 ggcgcacagc gccttcgatg cgctcgcgaa gggcggcaag gtcacgatgc cgcttgcgcc
     2101 gacgttctgg gccaggacct tcggcatgct caccgatcgc ttcggcgtca gctgggccgt
     2161 caacggcgag ccgatcccga tgaaatgacc ggagcgcagc ggtgaaatac ctctgtctgg
     2221 tctacctcga cgccgagaac tgggcggcct gcggcgacgc cgagtgcgca gactatgtgc
     2281 agcagctcgc cgacgggcgc cggttgctgg ccgccgagcc gctgcatcgc gcccacaccg
     2341 ccaccacggt gcgggtgcgc ggtggcgagg tgacgctctt cgacggccct ttcgccgaga
     2401 cgcgcgaaat gctcgccggc ttctacctcg tcgacgcgcg cgacctcaac gaggcgatcc
     2461 agctcgctgc ccgtatcccg ccagcgcgct acggcagcgt cgaagtgcgc ccggtgcgcg
     2521 agctgcagcc gtaggagccg gcctcctttt cattcgggga aggcgccagc gtcccgagct
     2581 tggcccggcg agtgcggcgg gaccgtcacg cttgaatgac gccgtcgcac gctcatctag
     2641 ggaagatgat ttctgcaggc gtcagcgccg cgctgcccat gcttctgttg gccgccgcgg
     2701 cgctcgtggc agtgacggcc ctgctggcac agccgtggct ggttcggcgc aggcgcgcgc
     2761 ggctgcgtgc cagggcgttt ccggcagcgt ggcgacggat cttgcgccgc cgggtgccgc
     2821 tcgtggcacg actgccgcca cacctgcagc tgcggctgaa gggccacatc caggtattca
     2881 ttgcggaaaa gcccttcatc ggctgcaacg ggcagcgcat caccgacgac gtgcgcgtca
     2941 cgatcgcggc ccaggcttgc cttctgctgc tcggccaccc gcagcccgat tgctttccgc
     3001 gcctgcgaca ggtcctggtg tatcccgatg ccttcgtcgc cgatcgcgaa cgacccgtcg
     3061 gtgcaggcct ggtgcaggca caacgccaca cgctggcggg cgagtcgtgg acccaggggc
     3121 aggtgattct ttcctgggca ga
//
LOCUS       DASRWS010000600         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1110828, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000600 DASRWS010000000
VERSION     DASRWS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110828"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..997
                     /locus_tag="VFF72_11495"
     CDS             71..997
                     /locus_tag="VFF72_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HZV93834.1"
                     /translation="MTLTELRYVVAVAREKHFGRAAEACSVSQPTLSVSIKKLEEELD
                     IKLFERGASEVSVTPLGEEIVRQAQAVIEGAAEIRETAKRRKDPLSGPLRLGVIYTIG
                     PYLLPDLVRQAIERVPQMPLMLQEHFTVKLLELLRTGELDCAIMAEPFPDTGLAIAPL
                     YDEPFVVAVPKTHPLARRKSISADELKREKMLLLGTGHCFRDHVLEVCPEYAQFASTD
                     AEGMRKTFEGSSLETIKYMVASGMGVTVVPQLSVPAEPQPHLAFVPFEKPVPTRRVVL
                     AWRRSFTRYEAIAELRNAIYACELKGVKRLTA"
     gene            1080..1703
                     /locus_tag="VFF72_11500"
     CDS             1080..1703
                     /locus_tag="VFF72_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZV93835.1"
                     /translation="MRIAIVTFDGFDEIDVFVPLGLLNRLSAQGWKAEITSPSANVTS
                     MNGVTVRAQHPLAYANDADAVLFGGGLYTRAIAQEIGKKTSLLDRLHLDPVRQTIGAQ
                     CSGSLLLARLGLLADMPACADATTRPWLMEAGVRVDDAPFHARGGVATAGGCLASQYL
                     AAWFMVRGAGRDAAARALSQAGPVGGKDDYVERLLEVVIPFVPDAAS"
     gene            1813..3438
                     /locus_tag="VFF72_11505"
     CDS             1813..3438
                     /locus_tag="VFF72_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZV93836.1"
                     /translation="MADLASEVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGSV
                     KARKASRHATSDWMEIEKQRGISVASSVMQMEYRGAVINLLDTPGHQDFSEDTYRVLT
                     AVDAALMVIDAANGVEPQTRRLLQVCRARNTPILTFVNKMDREVQEPLVLMDEIEREL
                     GMEVVPFTWPVGMGKRFHGVMDLRAAQMRVFRPGQDRDRDDDEIVAGLDNPVYAERFG
                     AAYAQAHDEIELLTDAAPPFDREAFLAGRQTPMFFGSAVNNFGVREVLDALVDLAPPP
                     GDRAAMQRTVHPDEAKFSGVVFKIQANMDPAHRDRIAFVRVASGHFERGMRLKVVRSG
                     KELRPNTVVSFLSQRRELLDEAFAGDIIGIPNHGVLQLGDTVTEGEALQYTGLPFFAP
                     EMFRSVEVADPLRTKQLRAGLTQLGEEGAIQVFRPVSGSVLLLGAVGQLQFEVVAHRL
                     EHEYGVKARILPSRYQVARWVTCEPEDGGAKELQRFIDANQHRVAYDAVDAPTVLVEY
                     APELRAIESNWPKVRFHALREHAGLVFQKQLEG"
     gene            3489..4391
                     /gene="ubiA"
                     /locus_tag="VFF72_11510"
     CDS             3489..4391
                     /gene="ubiA"
                     /locus_tag="VFF72_11510"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HZV93837.1"
                     /translation="MSTNAPRRIALYLDLIRWDRPAGTLLLLWPTLSALWIAAGGFPG
                     WHLLAVFVVGTFLMRSAGCCVNDVADREFDRHVKRTAARPVTSGAVGVAEALALGAAL
                     ALAAFALVLTTNTATVAWSVVALAVTLLYPFSKRVFAMPQAVLGIAFSFGIPMAFAAV
                     RGQDAASIGAIVASVPPLAWWLLLGNLFWVLAYDTEYAMVDRDDDLKIGIRTSAITLG
                     RFDVAAVMASYAVFLAVWAFLGRRLQFGVPYFIGLFCAAAIAGWHFLLIHSRSRDGCF
                     RAFRLNHWLGFAVFAGVVLDFAFR"
     gene            complement(4509..>4874)
                     /locus_tag="VFF72_11515"
     CDS             complement(4509..>4874)
                     /locus_tag="VFF72_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV93838.1"
                     /translation="TFGGNPLAAAVGIAALELLIGERLSERARVRGDELLAALRRIDH
                     PAIREVRGKGLLIGVEIDPAHATARQVCEGLLAAGVLSKDTHATVVRFAPPLIVESEH
                     IALAVAALESALRPFGRSQ"
BASE COUNT          719 a         1738 c         1636 g          781 t
ORIGIN      
        1 gtcgtcgcct gatgcccgcc cggcgttcgt cctcgcgccc atccgaaggc cggcccggga
       61 cggcgcgacc gtgacgctga ccgagcttcg ctacgtcgtt gccgtcgccc gcgagaagca
      121 cttcgggcgc gccgccgagg cgtgttcggt ctcgcagccg accctgtcgg tatcgatcaa
      181 gaagctcgag gaagaactcg acatcaagct cttcgagcgc ggcgccagcg aggtcagcgt
      241 cacgccgctc ggcgaggaga tcgtgcgcca ggcgcaggcc gtcatcgagg gcgcggccga
      301 gatcagggag acagccaagc ggcgcaagga tcccctgagc gggccgctgc gcctgggagt
      361 catctacacg atcggcccgt atctgctgcc cgacctcgtc aggcaggcga tcgagcgcgt
      421 gccgcagatg ccgctgatgc tgcaggagca cttcacggtc aagctgctcg aattgctgcg
      481 caccggtgag ctcgactgcg ccatcatggc cgagcccttt cccgacaccg gcctggcgat
      541 cgctccgctc tacgacgagc ctttcgtcgt cgccgtgccg aagacgcatc cgctcgccag
      601 gcgcaagtcg atcagcgccg acgagctcaa gcgcgagaag atgctgctgc tcggcacggg
      661 ccactgcttt cgcgaccacg tgctcgaggt ctgccccgag tacgcccaat tcgcgtcgac
      721 cgacgccgag ggcatgcgca agaccttcga gggctcgtcg ctcgagacga tcaagtacat
      781 ggtggcctcg ggaatgggcg tgacggtggt gccgcaactg tcggtgccgg cggagccgca
      841 gccgcacctg gccttcgtcc ctttcgagaa gcccgtgccg acgcgtcgcg tcgtcctcgc
      901 ctggcggcgc agcttcaccc gctacgaggc gatcgccgag ctgcgcaacg cgatctatgc
      961 ctgcgagctg aagggcgtca agcgcctgac cgcctgagcc cgaggcggcc ctgccgcgag
     1021 cgagggcggc ccgagcgttc ccctcgacgc cgcccgcgct ggcatacgat gacgcagcca
     1081 tgcgcatcgc gatcgtcacc ttcgacggct tcgacgagat cgatgtcttc gtcccgctcg
     1141 gcctgctcaa ccggctgtcg gcacagggct ggaaagcgga gatcaccagc ccgtcggcga
     1201 acgtcacgtc gatgaacggc gtcaccgtgc gggcacagca tccgctcgcc tacgccaacg
     1261 acgccgatgc cgtgcttttc ggcggcggcc tctatacgcg ggcgatcgcc caggagatcg
     1321 gcaagaagac ctcgcttctc gatcggctgc atctcgatcc ggtgcgccag acgatcggtg
     1381 cgcagtgttc gggcagtctc ctcctggccc ggctcgggct gctcgccgac atgccggcgt
     1441 gcgccgacgc gacgacccgg ccctggttga tggaggccgg cgtccgggtc gacgacgccc
     1501 cgttccacgc gcgcggcggc gtcgccaccg ccggcggatg cctggcgtcg caatatctcg
     1561 cggcgtggtt catggtgcgc ggtgcgggcc gcgacgcggc ggcccgggcg ctgtcgcagg
     1621 ccggcccggt cggcggcaag gacgattacg tcgagcgcct cctcgaggtg gtgatcccct
     1681 tcgttcccga cgcggcgagc tgagccacgc cgcgcgcttc gatcgcacgg caggctgcgc
     1741 ggccagcccg tgccgcgacc ggcgccgata atcgcccgct ttcctgcatg ccggccgtcc
     1801 cgcggcccga ccatggccga cctcgcctcc gaagtgcgcc gccgacgcac cttcgccatc
     1861 atttcccacc ccgacgcggg caagacgacg ctgacggaaa agctcctgct cttctccggc
     1921 gcgatccaga tcgccggctc ggtgaaggcg aggaaggcct cgcgccacgc cacgtccgac
     1981 tggatggaaa tcgaaaagca gcgcggcatc tcggtcgcct cgagcgtcat gcagatggag
     2041 taccgcggcg ccgtcatcaa cctgctcgac acgccgggcc accaggactt ctcggaagac
     2101 acctaccgag tcctcactgc ggtcgatgcc gcgctgatgg tgatcgacgc cgccaacggc
     2161 gtcgagccgc agacgcggcg gctgctccag gtctgccggg cccgcaacac gccgatcctc
     2221 accttcgtca acaagatgga ccgcgaggtg caggagccgc tcgtgctgat ggacgagatc
     2281 gagcgcgagc tcggcatgga ggtggtgccg ttcacctggc cggtcggcat gggcaagaga
     2341 ttccacggcg tgatggacct gcgcgccgcg cagatgcgcg tcttcaggcc ggggcaggac
     2401 cgcgaccgcg acgatgacga gatcgttgcc ggactcgaca acccggtcta tgccgagcgc
     2461 ttcggcgcgg cctacgcgca ggcgcacgac gagatcgagc tcctgaccga cgcggcgccg
     2521 ccgttcgatc gcgaagcctt cctcgccggc cggcagacgc cgatgttctt cggctcggcc
     2581 gtcaacaact tcggcgtccg cgaggtgctg gatgcgctcg tcgatctcgc tccgccgccc
     2641 ggcgaccgcg ccgcgatgca gcgcacggtg catccggacg aggcgaagtt cagcggcgtc
     2701 gtcttcaaga tccaggccaa catggatccg gcgcaccgcg accgcatcgc cttcgtccgc
     2761 gtcgcctcgg gccacttcga gcgcggcatg cggctgaagg tcgtgcgctc gggcaaggag
     2821 ctgcgcccga acaccgtcgt cagctttctc tcgcagcgac gcgagctgct cgacgaagca
     2881 ttcgccggcg acatcatcgg catccccaac catggcgtgc tgcagctcgg cgacacggtg
     2941 accgaaggcg aggccctgca atacaccggg ctgcccttct tcgcgccgga gatgttccgc
     3001 tcggtcgagg tcgccgatcc gctgcgcacg aagcagctgc gcgccgggct gacgcaactg
     3061 ggcgaggaag gcgcgatcca ggtctttcgc ccagtgtccg gctcggtgct cctgctcggc
     3121 gcggtcgggc agcttcagtt cgaggtggtg gcgcaccggc tcgagcacga atacggcgtc
     3181 aaggcgcgca tcctgccgag ccgctatcag gtcgcgcgat gggtgacctg cgagcccgag
     3241 gacggcggcg cgaaggagct gcaacgcttc atcgatgcca accagcatcg cgtcgcctac
     3301 gacgccgtcg atgccccgac cgtgctcgtc gagtacgcgc cggagttgcg cgccatcgag
     3361 agcaactggc cgaaggtccg cttccacgcc ctgcgcgagc acgccgggct ggtcttccag
     3421 aaacagctcg aaggctgagc gtgaccggat gacgcgccga gtcgagcgat gagctggcat
     3481 cgcggcccat gagcacgaac gcaccccggc ggatcgccct ctacctcgac ctgatccgct
     3541 gggatcgccc tgcgggcacg ctgctgctgc tctggccgac gctttcggcg ctgtggatcg
     3601 ccgcgggcgg ctttcccggc tggcatctgc ttgccgtctt cgtcgtcggc acctttctca
     3661 tgcgcagcgc cggttgctgc gtcaacgatg tcgccgaccg ggagttcgat cgacacgtca
     3721 agcgcacggc ggcgcggccg gtgacaagcg gcgcggtcgg cgtcgccgag gcgctggcgc
     3781 tgggcgcggc gctggcgctc gccgcattcg ccctcgtgct gacgacgaac acggctaccg
     3841 tggcctggag cgtcgtcgcg ctcgcagtga cgctgctcta tccgttcagc aagcgcgtct
     3901 tcgcgatgcc gcaggccgtt ctcggcatcg ccttcagctt cggcatcccg atggcgttcg
     3961 cggcggtccg cggccaagac gccgcgagca tcggcgcgat cgttgccagt gttccgccgc
     4021 tggcctggtg gctcttgctg ggcaaccttt tctgggtgct cgcctacgac accgaatatg
     4081 cgatggtcga tcgcgacgac gacctgaaga tcggcatccg gacctcggcc atcacgctcg
     4141 gccgcttcga tgtcgccgcg gtgatggcgt cctacgccgt gtttctcgcc gtctgggcct
     4201 tcctcggtag acgacttcag ttcggtgtgc cgtatttcat cggccttttc tgcgcggcgg
     4261 cgatcgccgg atggcacttc ctgctcatcc actcgcgcag ccgcgatggc tgctttcgcg
     4321 ccttccgcct gaaccactgg ctgggctttg ccgtgttcgc cggcgtcgtt ctcgatttcg
     4381 cctttcgctg acagtcgccg catggggact cggtgtcgag ctcgggcgac acgcggcccc
     4441 ggcccgacgc cctcttcgag cggttcaggg gcgatcccgc gcatcgacgc acgaagcgcg
     4501 cgggccgctc actgcgagcg gccgaacggc cgcagcgccg actcgagcgc ggccaccgcg
     4561 agcgcgatgt gttcgctttc gacgatgagc ggcggcgcga agcgcaccac cgtggcatgc
     4621 gtgtccttgc tgagcacgcc ggcggcgagc aggccctcgc agacctgtcg ggcagtcgca
     4681 tgcgccgggt cgatctcgac gccgatcaaa agccccttgc cacggacctc ccggatcgcc
     4741 ggatggtcga tgcgacgcag cgcggcgagc aactcgtcgc cgcgcacgcg agcccgctcg
     4801 gaaagccgct cgccgattaa cagctcgagc gcggcgatgc ccacggccgc ggcgagcgga
     4861 ttgccgccga aggt
//
LOCUS       DASRWS010000601         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3974757, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000601 DASRWS010000000
VERSION     DASRWS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3974757"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFF72_11520"
     CDS             complement(<1..458)
                     /locus_tag="VFF72_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93839.1"
                     /translation="MSIEPATLQAFKRAKELSDRLKAARPQIEKRLTALMDKLAKATD
                     DLDADAIKLYLKALDGEKHLLTTAMQSVVVATANIEEVEDDEEFLNTRLKDLEAVKGP
                     VDEARKSFDRRYADAMAIEKMAKDATKKAGQANTRLIERLAALDKLIADV"
     gene            complement(533..>2431)
                     /gene="ligA"
                     /locus_tag="VFF72_11525"
     CDS             complement(533..>2431)
                     /gene="ligA"
                     /locus_tag="VFF72_11525"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZV93840.1"
                     /translation="PELASPDSPTRRVIGEVLEGLRPVHHAVPMLSIDTETDTTSAGA
                     EKFDARVRKALGLVDADPPVVYAAEMKFDGLAISLRYERGALVRAATRGDGETGEDVT
                     HTVRTIHDVRHRLKGVDAPVLEIRGEVFMRRDRFEALNERQRTLIEAGAKNEKTFVNP
                     RNAAAGVVRQLDARNAARRPLNFFAYALGEIEGWAPPPTQTALLEAFAAMGLPASEHH
                     ARVEGAAGLVRFHAEVAARRDALPYDIDGVVYKVDDRSLQERLGFKSREPRWAVAQKY
                     PAQEKTTRLNGIEVQVGRTGKLTPVAKLEPVFVGGTTVSNATLHNLFELRRKGVRIGD
                     RVIVRRAGDVIPEIVGRIPGPRPAYVANFRMPPVCPVCGSRVVREKGGVEHRCSGGLF
                     CAAQRKIAILHFAGRRALDIEGLGDKIVDQLVDGGIVETLPGLYRLTQGDLQALERMG
                     ERSAANLIAAIEASKSPSLGRFLYALGIRHVGETTGKDLARHFGTLAAVEAAGLDALL
                     EVPDVGPIVAQSVHAFFREPHNVEVVRALLAAGVSPQESEPPTRAAALPFAGRTFVIT
                     GTLPTLSRDEAKDMIESLGGKVAGSVSKKTDWVVAGAEAGSKLARAEALGVAVIDESR
                     LLALLDGR"
BASE COUNT          341 a          854 c          815 g          421 t
ORIGIN      
        1 tcgacgtcgg cgatgagctt gtcgagcgcc gccaggcgct cgatcaacct ggtattcgcc
       61 tggcccgcct tcttcgtcgc gtccttggcc atcttctcga tcgccatcgc gtcggcatag
      121 cgtcggtcga acgatttcct cgcctcgtcg accggcccct tgacggcctc gagatccttc
      181 agccgggtgt tgaggaactc ctcgtcgtcc tcgacctctt cgatgttggc ggtggcgacc
      241 accaccgact gcatcgccgt ggtgagaagg tgcttctcgc cgtcgagcgc cttcaggtag
      301 agcttgatgg cgtcggcgtc gaggtcgtcg gtggccttgg ccagcttgtc catcagggcg
      361 gtcagccgct tctcgatctg cgggcgcgcc gccttgagcc tgtccgagag ttccttggcc
      421 ctcttgaatg cctggagggt ggcgggttcg atcgacatga cgactcctct atgaacgtcg
      481 gcattctctc cggcgcgaat gccggcgtca tcccccccaa gcgagtctgc cgtcagcgtc
      541 cgtcgagcag ggccagcagt cggctttcgt cgatcaccgc gacgccgagc gcctcggccc
      601 tcgccagctt cgatccggct tcggcaccgg cgaccaccca gtccgtcttc ttcgataccg
      661 agccggcgac cttgccgccc agcgattcga tcatgtcctt cgcctcgtcg cggctgaggg
      721 tcggcagcgt gccggtgatg acgaaggtcc tgccggcaaa gggcaacgcc gccgcacgcg
      781 tcggcggttc gctctcctgg gggctgacac cggccgcgag caacgcacga acgacctcga
      841 cgttgtgcgg ctcgcgaaag aaggcgtgca cgctttgcgc gacgatgggg ccgacgtcgg
      901 gcacctcgag caaggcatcg agaccggccg cctcgaccgc tgccagggtg ccgaagtggc
      961 gagcgaggtc cttgcccgtc gtctcgccga cgtggcggat gcccagcgcg tagagaaagc
     1021 ggccgagcga cggcgacttg ctcgcctcga tcgcggcgat gaggttggcg gcactgcgct
     1081 cgcccatccg ctcgagcgcc tgcaggtcgc cttgcgtcag ccgatagagg ccgggcagcg
     1141 tctcgacgat gccgccatcg acgagctggt cgacgatctt gtcgcccagg ccctcgatgt
     1201 cgagcgcgcg ccggccggcg aaatgcagga tcgcgatctt gcgttgcgcc gcgcagaaaa
     1261 gcccgccgct gcatcgatgc tcgacgccgc ccttctcgcg cacgacccgg ctgccgcaca
     1321 ccgggcagac agggggcatg cgaaagttgg ccacgtacgc cggacgcggg cccggaatgc
     1381 gaccgacgat ctcggggatg acgtcgccgg cgcgccgcac gatgacgcgg tcgccgatgc
     1441 gcacgccctt gcgacgcagc tcgaagaggt tgtgcagcgt cgcgttcgaa acggtcgtgc
     1501 cgccgacgaa gaccggctcc agcttggcga ccggcgtcag ctttccggtg cgaccgacct
     1561 gcacctcgat gccgttgagc cgcgtcgtct tttcctgcgc cggatatttc tgtgcgacgg
     1621 cccagcgtgg ctcgcgcgac ttgaagccga gccgctcctg caacgatcga tcgtcgacct
     1681 tgtagacgac gccgtcgatg tcgtagggaa gcgcgtcgcg ccgggccgcg acctcggcat
     1741 ggaaccgcac caaccccgcc gcgccctcga cgcgggcgtg gtgctcgctt gccggcaggc
     1801 ccatcgccgc gaacgcctcg agcagcgcgg tctgcgtcgg cggcggcgcc cagccttcga
     1861 tttcgccgag cgcgtaggcg aagaaattga gaggtcgcct ggccgcgttg cgcgcgtcga
     1921 gctggcgcac cacgccggcc gccgcgttgc gcggattgac gaaggtcttc tcgttcttcg
     1981 cgccggcctc gatcagtgtt ctctgtcgct cgttgagcgc ctcgaagcgg tcgcgacgca
     2041 tgaacacctc gccgcggatc tcgagcaccg gcgcatcgac gcccttcagg cgatgccgga
     2101 cgtcgtggat cgtccgtacc gtgtgcgtca cgtcctcgcc ggtctcgccg tcgccgcgtg
     2161 tcgcggcgcg gaccagcgcg ccgcgctcgt agcgcaggct gatcgccagg ccgtcgaact
     2221 tcatctcggc ggcgtagacg acgggcggat cggcgtcgac caggccgagc gccttgcgca
     2281 cccgcgcatc gaacttctcc gcacctgccg atgtcgtgtc ggtctcggtg tcgatcgaaa
     2341 gcatcggcac ggcgtgatgc acggggcgca agccctcgag gacctcgccg atgacgcgcc
     2401 gggtaggcga atccgggctc gccagctcgg g
//
LOCUS       DASRWS010000602         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1111663, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000602 DASRWS010000000
VERSION     DASRWS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111663"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFF72_11530"
     CDS             complement(<1..576)
                     /locus_tag="VFF72_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZV93841.1"
                     /translation="MPSELITERRGSTLILTISDPPTRNTLSAQVIAAGIEALGASES
                     NDAVRTVVVRGQGAAFCAGGNLNGLRERRAAGRDAQLQMLEHLHHFMQTIRAYPKPVL
                     ACVEGTAAGAGFSLALACDMIVAASDARFVMSYAKIGLTPDGGATWGLAASLPRALAL
                     EMLWLARPVSAETLHAHGLVNEIAPSGRAIDA"
     gene            735..2705
                     /locus_tag="VFF72_11535"
     CDS             735..2705
                     /locus_tag="VFF72_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93842.1"
                     /translation="MQRKFILLAMAAAAFAMAPEAMAMGFGQTALRTTLGQPLDFSAA
                     LTFDGDETLAPQCVDAEVRAGDARVPPPEVHASLEPARGTMPRSIRVTTTARIDEPVA
                     TIDITVGCSSKMSRRFVVFVDPPPVRLAEASPASQPLPPQTLRRDNQSILLGDIARAA
                     DASRRGGSVAGAGGEQEDGARTRGTAATASSTAPRSIARAGRAAARSSEGSQARASRH
                     RHGRTHFASRSAPRSGRLATASRPRLQLEPPVALAAPQAAASAPPRPETPAVDVAVAA
                     AAAASSALQRERDRIQVLEAALAGLRSDSQATQRSVTALQARLREAESQRYPGGLVYV
                     LAGMVVFLGLVIAVLVWLRPRQRQRARWFDAAAEQQARAARAAANDAPLSRAPESPVS
                     TVAATGWRSSGPGSMAGADTLPAAIGGLEVTTVLGPEAARPVVESMRGATGRRAGELS
                     MEELIDLEQQAEFFAVLGQDEAAIGLLDAYLHGEGGKSPLPYLQLLELHQRRGDKETY
                     EAVRGAFNDRFHAFAPEWSDDVHYGRDLEAYPQTVARLQALWPTPMSAMQALDSLLFR
                     RHASDDTFDFPAYRELLFLYSIARELSGHVETDYGSIDVLLPLEDAPIDLTPTTPDGG
                     AFSVDLDVSSWDEAVPAEPLVIRRSPGRRGAA"
     gene            complement(2751..>3326)
                     /locus_tag="VFF72_11540"
     CDS             complement(2751..>3326)
                     /locus_tag="VFF72_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV93843.1"
                     /translation="PTDVLVISDEVYEHMVYEGRHESVARLPGLAARSVVVSSFGKTY
                     HVTGWKVGYAAAPAPLATEFRKVHQFNVFTVNTPMQHGIAAFMAEPSHHLGLGAFYRA
                     KRDLFRAGLARTRLRALPCDGTYFQCVDYSRVSDLGEADFCRWLTSEIGVAAIPVSAF
                     YIDGFEQRIARFCFAKKNETLELALDRLAAI"
BASE COUNT          483 a         1185 c         1166 g          492 t
ORIGIN      
        1 tgcgtcgatc gcccgacccg acggtgcgat ctcgttgacc aggccgtgcg cgtgcagcgt
       61 ctccgccgac accggccgtg ccagccacag catctcgagc gcgagggcgc gcggcagcga
      121 ggccgcgagc ccccatgtcg cgccgccgtc gggcgtcagg ccgatcttcg cgtacgacat
      181 gacgaagcgt gcgtccgacg cggccacgat catgtcgcag gcgagtgcga gcgaaaaccc
      241 ggcgccggcc gcagttcctt cgacacaggc cagaaccggc ttgggatacg cgcggatcgt
      301 ctgcatgaag tgatgcaggt gctcgagcat ctgcaactgg gcgtcgcgcc cggcggcgcg
      361 acgttcgcgc aatccgttca ggttgccgcc ggcgcaaaac gccgcgccct ggccgcgcac
      421 gaccaccgtt cgcaccgcgt cgttcgactc cgacgcgccg agggcttcga tgcccgcggc
      481 aatcacttgt gccgagagcg tgttgcgggt cggcggatcg ctgatcgtca ggatcagcgt
      541 gctgccgcga cgctcggtga tcagttcgga aggcatggaa aaacggccct gttcatcgcg
      601 actcggcaga ggtcaaatgc aggcacttcc attcgcggaa ttgtgtcacg cagcgctgcg
      661 cggcgaggcc tcgctgcggg cggcgcgcgg ggggtcgcat gctagagtcg tttcgacccc
      721 cgagcctcga ccgaatgcag cgcaaattca ttctcctcgc catggctgcc gcggccttcg
      781 cgatggcacc cgaggcgatg gcgatgggat tcgggcagac cgcgttgcga acgacgctgg
      841 gccagccgct cgacttcagt gccgcgctca cgttcgatgg cgacgagacg ctggcgccgc
      901 agtgcgtcga cgccgaggtt cgcgccggcg acgcgcgcgt tccgccgccc gaggtgcacg
      961 ccagcctcga gccggcccgc ggcaccatgc cgcggtccat ccgcgtgacg acgacggcgc
     1021 gcatcgacga gccggtggcg acgatcgaca tcaccgtggg ctgcagctcg aagatgtcgc
     1081 gccgcttcgt cgtcttcgtc gatccgccac cggtgcgcct cgccgaagct tccccggcgt
     1141 cgcaaccgct gccgccgcag acgctgcgac gcgacaacca gtcgatcctg ctgggcgaca
     1201 tcgctcgtgc cgccgacgca tcgcgtcgag gcggctccgt cgccggcgcg ggcggcgagc
     1261 aagaagacgg agcgcggacg cgtggcacgg cagcgaccgc gtcgtcgaca gctccgcgat
     1321 cgatcgcccg ggccggccgg gccgccgctc gctcgagcga aggttcgcag gcgcgagcgt
     1381 ctcgtcaccg ccatggccgc acccacttcg cgtcgcggtc cgcgccgagg tcgggtcgcc
     1441 tcgccactgc atcccggccg agactgcagc tggagccgcc ggtggcactc gcggcgccgc
     1501 aggcggccgc atcggcgccg ccgcgtccgg agacgccggc cgtcgatgtc gcggtggccg
     1561 ctgcagccgc tgcctcgtcg gcattgcagc gtgagcgcga tcgcatccag gttctagaag
     1621 ccgccctcgc cggtctgcgc agcgactcgc aggcgaccca gcgcagcgtc accgcgctgc
     1681 aggcgcgact gcgcgaagcc gaaagccagc gctacccggg cggcctcgtc tacgtgctgg
     1741 ccgggatggt cgtcttcctg ggcctcgtca tcgccgtgct cgtctggctt cgaccgaggc
     1801 agcgccagcg cgcgcgctgg ttcgacgccg cggcagagca acaggcacgc gccgcccgcg
     1861 ccgccgcgaa cgacgcgccg ttgagccgcg cgcccgaatc acccgtgtcg accgtggccg
     1921 ccaccggctg gcggtcttcc ggtccgggca gcatggccgg cgccgacacg ctgcccgccg
     1981 cgatcggtgg cctggaagtg acgacggtgc tcggccccga ggcggcgcgt ccggtcgtcg
     2041 aatcgatgcg tggcgcgacg ggtcggcgcg ccggcgagct gtcgatggag gagctgatcg
     2101 atctcgagca gcaggccgag ttcttcgccg tcctcggaca ggacgaagcg gcgatcgggt
     2161 tgctcgacgc gtacctgcac ggcgagggcg gcaagagtcc gctgccgtac ctgcaactgc
     2221 tcgagctgca ccagcggcgt ggcgacaagg agacctacga agcggtgcgc ggcgccttca
     2281 acgaccgctt ccatgccttc gcccccgagt ggtcagacga cgtccactac ggccgcgacc
     2341 tcgaggccta tccgcagacc gtggcccggc tgcaggcgct ctggccgacg cccatgtcgg
     2401 cgatgcaggc gctcgacagc ctgctcttca ggcgtcacgc gagcgacgac acgttcgact
     2461 ttccggccta tcgcgagctg ttgttcctct actcgatcgc gcgcgagctt tccggccacg
     2521 tcgagaccga ttacggatcg atcgacgtct tgctgccgct cgaggacgcg cccatcgatc
     2581 tgacgccgac cacgcccgac gggggcgcct tctcggtcga tctcgacgtc tcgagctggg
     2641 acgaggcagt gccggccgag ccgctcgtca tccgccgctc gcccggacgc cgcggcgctg
     2701 cctgaccgcg gcgtcggttg cgtcgctctt gccgcgactt cggcgccggc tcagatcgcg
     2761 gccaggcgat cgagcgcgag ctcgagcgtc tcgttcttct ttgcgaagca gaagcgggcg
     2821 atgcgctgtt cgaagccgtc gatgtagaac gccgacacgg ggatggcggc gacgccgatc
     2881 tcgctcgtca gccagcgaca gaaatcggct tcgcccaggt cgctgacccg cgagtaatcg
     2941 acgcactgga agtaggtgcc gtcgcagggc agcgcgcgca ggcgcgtccg cgccaggccg
     3001 gcgcgaaaga ggtcgcgctt ggcgcgatag aacgcgccca gtccgagatg gtgcgacggc
     3061 tcggccatga acgcggcgat cccgtgctgc atcggcgtgt tgaccgtgaa gacgttgaac
     3121 tggtggacct tgcggaactc ggtggcgagc ggcgccggcg ccgccgcgta gcccaccttc
     3181 cagcccgtga cgtgataggt cttgccgaag ctcgagacga cgacgctgcg ggccgcgagg
     3241 ccgggaagcc gggcgacgct ctcgtgcctg ccttcgtaca ccatgtgctc gtagacctcg
     3301 tcggagatga cgagcacgtc ggtcgg
//
LOCUS       DASRWS010000603         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1113111, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000603 DASRWS010000000
VERSION     DASRWS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113111"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1048
                     /locus_tag="VFF72_11545"
     CDS             47..1048
                     /locus_tag="VFF72_11545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HZV93844.1"
                     /translation="MFKALLLEKDDRGFRAGVREVDEAGLPEGDVDVRVAHSTLNFKD
                     GLAITDRSPVVRAWPMVAGIDGAGTVVASTHADWKPGDRFVHNGWGVGETRWGCLAER
                     ARLKGDWLVRLPDVFSTRQAMAIGTAGYTAMLCVMALERHGLRAGDGTVLVTGATGGV
                     GSVAVALLARLGHRVAAATGKQEEGDYLRRLGATEVIDRKDLAAPGKPLQKERWSAVV
                     DAVGSHTLVNALAQTRYGGVVAACGLAQGFDLPATVMPFILRGVTLAGVDSVMAPLAV
                     RREAWSRLARDLDVAALDLITEEVPLARAIDKAGELMAGRVRGRVVVAISEPAAAAA"
     gene            complement(1020..1559)
                     /locus_tag="VFF72_11550"
     CDS             complement(1020..1559)
                     /locus_tag="VFF72_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZV93845.1"
                     /translation="MHMLCDPADSVLLIIDTQVQLMPAIHDSENVVRRCVQLAAAARE
                     LGIHVIGTEENPPGLGPLVPEVAALCDTTLSKSHFSAPAEPGFLDRLPAGRNSYVVAG
                     CEAHVCVMQTTRGLLAAGHSVKWVADAVGSRQPHNRLAATERARHFGADVVTTEMVIF
                     EWLGTCEHPKFKRLLQLVR"
     gene            1664..3223
                     /locus_tag="VFF72_11555"
     CDS             1664..3223
                     /locus_tag="VFF72_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93846.1"
                     /translation="MPMTGAEYRESLRRARPRVWIDGHAIESVADAPQLAPGINALAV
                     SYDFAMRDELAPLMRAVQRSSGKTVNRMLHVDTSSADLLNKLEAVRVLCQETGCAQRY
                     LAHDAFSALAQTTARVDADKGSRYAERFTAWLHDFQDRDLAVGIAMTDAKGDRSRRPH
                     EQANPDSYVHIVERNARGIVISGTKAIVTGAPYMHEFLVMPCRSMIEADADFAVCCAV
                     PCDAEGVTIVARPAGRPGEKAALFSGRYGQSTGVVMFDRVAVPWERVFLAGEWEHSAH
                     LTYGYATHHRHTCIAARAGFGDLLIGAGALMCEANGFDPGAETHLREQMVQLITIVEG
                     FYACGVAASVYAREEHGVVVPDPVFANIGKLLLANQIYEMQRIAHYVSGGLVVTLPGP
                     DEDHNPATAASLADVLRANPDVPYDKRIEAARLVEDLTASYQGGWYSVISLHGGGSPA
                     AMKQEIWRNYPVGSKVELVERLLDRGLVADTNRPIMAHRQPGRCCDSGCTSASATTMV
                     ALPATRRGSTS"
     gene            3220..>4776
                     /gene="prpE"
                     /locus_tag="VFF72_11560"
     CDS             3220..>4776
                     /gene="prpE"
                     /locus_tag="VFF72_11560"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HZV93847.1"
                     /translation="MKLQSTGRYADFYRRSIESPDAFWAEQAALVDWHRPFDSVCDAS
                     RPPFVRWFAGGLTNLCHNAVDRHLATRASQPALIHVSTETADGSPKERVYSFSELHDE
                     VQRMAAILQGLGVGKGDRVLLYMPMIAEAAFAMLACARIGAIHSVVFGGFASVSLANR
                     IDDAEPVVVVSADAGSRAGKVIAYKPLLDEAIGMSRHKPRSLLLVDRGMAPMSRVAGR
                     DHDYAALRRHHLDAKVPCVWVDATHPSYTLYTSGTTGQPKGVQRDTGGYAVALAASMK
                     HIFCGHAGETYFSTSDIGWVVGHSYIVYGPLLAGMATIMYEGLPIRPDAAIWWSLVER
                     HKVTAMFTAPTAIRVLKKQDPAFLRRHDLSSLRALFLAGEPLDEPTASWIAAAIGKPV
                     IDNYWQTESGWPILTVANGVEKMTSRFGSPGVPMYGFDVRLVDESTGKDIVEPDRKGV
                     VVIDGPLPPGFMQTVWRDDARYVSTYWKSVPGRMVYSTFDWGIRDEDGYYFILGRSDD
                     VINVAGHRLGT"
BASE COUNT          764 a         1653 c         1630 g          729 t
ORIGIN      
        1 gctatcgttg gccgatcagc caccctcgga tgcgtacgga gccaccatgt tcaaggcctt
       61 gcttctggaa aaggacgacc ggggattccg ggccggcgtc cgcgaagtcg acgaggccgg
      121 cctgcccgag ggggacgtcg acgttcgcgt cgcccattcg acgctgaatt tcaaggacgg
      181 cctcgccatc accgaccgca gccccgtcgt gcgggcctgg ccgatggtcg ccggcatcga
      241 cggcgccggc acggtcgtcg cctcgaccca cgccgactgg aagcccggcg atcgcttcgt
      301 gcacaacggc tggggcgtcg gcgagacgcg ctggggatgc ctcgccgagc gggcgcggct
      361 gaaaggcgac tggctggttc gcctgcccga tgtgttctcg acccggcagg cgatggcgat
      421 cggcaccgcc ggctacaccg cgatgctctg cgtgatggcg ctcgagcggc acggtcttcg
      481 cgctggcgac ggcacggtgc tcgtcacggg ggcgacgggt ggcgtcggca gcgtcgccgt
      541 cgccctcctg gcgcggctcg gccatcgggt cgccgccgcg accggcaagc aggaggaggg
      601 cgactacctg aggcgcctgg gcgccaccga ggtcatcgac cggaaggacc tggcggcacc
      661 cggcaagccg ctgcaaaagg agcgctggag cgcggtcgtc gatgccgtcg gcagccacac
      721 gctggtcaat gcgctggccc agacccgcta cggtggtgtc gttgccgcct gcggtctggc
      781 gcaaggattc gacctgcccg cgacggtcat gccgttcatc ctgcgcggcg tcacgctcgc
      841 tggcgtcgac agcgtcatgg cgccgcttgc cgttcgtcgc gaggcctgga gtcgtctcgc
      901 acgcgatctc gacgtcgctg cgctcgacct gatcaccgag gaggtgccgc tcgcgcgagc
      961 gatcgacaag gccggcgagc tgatggccgg ccgcgtgcgc ggccgggtgg tcgtcgcgat
     1021 cagcgaacca gctgcagcag ccgcttgaac ttcggatgct cgcaggtgcc cagccactcg
     1081 aagatcacca tctcggtggt gacgacgtcg gcgccgaagt gccgcgcccg ctcggtggcg
     1141 gcgagccggt tgtgcggctg gcgcgagccg accgcgtcgg cgacccactt caccgaatgg
     1201 ccggcggcga gaaggccacg tgtcgtctgc atgacgcaga catgggcttc gcaacccgcc
     1261 accacgtagc tgttgcggcc tgccggcagc cggtcgagaa agccgggctc ggccggtgcg
     1321 gagaaatgag acttcgacag cgtcgtgtcg cagagcgccg cgacttccgg gacgagcggg
     1381 ccgagccccg gcggattttc ttccgtgccg atgacatgga tgccgagctc acgcgcagcg
     1441 gcggccagct gcacgcagcg gcgaacgacg ttctcgctgt cgtggatcgc aggcatcagt
     1501 tgcacctgcg tgtcgatgat cagcaggacg ctgtcggcgg gatcgcagag catgtgcatc
     1561 gaaaggcttt caggtcggcg gcaaggcaat tttccgccat gacgcaacgg tgcgacgctg
     1621 cgtcgcgaca atcggcatgc gagacctgtc cgaaggagat gccatgccaa tgaccggcgc
     1681 cgagtatcgc gagtcgctgc gccgcgcgcg accgcgtgtc tggatcgacg gccacgccat
     1741 cgaaagcgtt gccgacgcgc cgcagctggc gcccggcatc aacgcgctgg ctgtcagcta
     1801 cgacttcgcc atgcgcgacg aactggcgcc gctgatgcgg gcagtgcagc gctcgagcgg
     1861 caagaccgtc aatcggatgc tgcacgtcga cacgagcagc gcagacctgc tgaacaagct
     1921 ggaggcggtt cgcgtcctct gccaggaaac agggtgcgcg caacgctatc tggcccatga
     1981 cgccttcagc gcgctggccc agaccaccgc gcgcgtcgac gccgacaagg gcagtcgcta
     2041 cgccgagcgt ttcaccgcgt ggctgcacga cttccaggat cgcgaccttg ccgtcggcat
     2101 cgccatgacc gacgccaagg gcgaccggag ccggcggccg cacgagcagg ccaatcccga
     2161 cagctacgtg cacatcgtcg aacgcaacgc cagaggcatc gtcatctcgg gcaccaaggc
     2221 gatcgtcacc ggcgcgccct acatgcacga gtttctcgtc atgccatgcc gcagcatgat
     2281 cgaggccgac gccgacttcg cggtctgctg cgcggtgccc tgcgacgccg aaggcgtcac
     2341 catcgtcgcc cgcccggccg gacgacccgg cgagaaggcg gcgctgtttt ccggccgcta
     2401 cgggcagtcg acgggcgtcg tcatgtttga ccgcgtcgcc gtcccgtggg agcgtgtctt
     2461 tctcgctggc gaatgggagc attcggcgca tctgacctac ggctatgcga cgcatcaccg
     2521 acacacctgc atcgcggcac gcgccggctt cggcgacctg ctgatcggcg cgggagcgct
     2581 catgtgcgag gcgaacggct tcgatccggg cgccgagacg cacctgcgcg agcagatggt
     2641 gcagctgatc accatcgtcg agggcttcta tgcgtgcggc gtcgcggcaa gcgtctatgc
     2701 gcgcgaggag cacggggtcg tcgtgccgga tccggtgttc gccaacatcg gcaagctgct
     2761 gctggccaac cagatctacg agatgcagcg catcgcccac tacgtctcgg gcggtcttgt
     2821 cgtgacgctt cccgggcccg acgaagatca caatcccgcc accgccgcca gcctcgccga
     2881 cgtgctgcgc gccaaccccg acgtgcctta cgacaagcgc atcgaggcgg cccgtctcgt
     2941 cgaggacctg acggcttctt accagggagg ctggtactcg gtgatcagcc tgcacggcgg
     3001 cggctcgccg gcggcgatga agcaggagat ctggcgcaac tatccggtcg gcagcaaggt
     3061 cgagcttgtc gagaggctgc tcgaccgcgg ccttgtcgcc gacacgaacc ggccgatcat
     3121 ggcccaccgg caacccggtc gctgctgcga cagcggttgc acgtcggcaa gtgcgacgac
     3181 gatggtcgcc ctgccggcga cccgacgagg aagcacgtca tgaagctgca atccaccggc
     3241 cgctatgccg atttctaccg ccgctcgatc gagtcgcccg acgccttctg ggccgagcag
     3301 gccgcgctcg tcgactggca caggccgttc gacagcgtct gcgacgcgag ccggccgcct
     3361 ttcgtgcgct ggttcgccgg cggcctgacg aacctgtgcc acaacgccgt cgaccgtcac
     3421 ctggcaacgc gcgcgtcgca gccggcgctg atccatgtct cgacagagac cgccgacggc
     3481 tcgccgaagg agcgcgtcta ctccttctcg gagctgcacg acgaagtgca gcgcatggcg
     3541 gcgatcctgc aaggcctggg cgtcggcaag ggcgatcgcg tccttctcta catgccgatg
     3601 atcgccgagg cggccttcgc gatgctcgcc tgcgcgcgga tcggcgcgat ccactccgtc
     3661 gtcttcggcg gcttcgcgag cgtgagcctg gccaaccgga tcgacgatgc cgagccggtc
     3721 gtcgtcgtca gcgccgacgc cggcagccgc gccggcaagg tcatcgccta caagccattg
     3781 ctcgacgagg cgatcggcat gtcgcgccac aagccgcgca gcctcctgct cgtcgatcgg
     3841 ggcatggcgc cgatgtcgcg cgtggccggc cgcgaccacg actacgcggc gctgcgccgt
     3901 caccatctcg atgcgaaggt gccttgcgta tgggtcgacg cgacacatcc gagctacacg
     3961 ctctacacca gcggcaccac cggccagccg aagggcgtgc agcgcgacac cggcggctac
     4021 gcggtggcgc tggccgcgag catgaagcac atcttctgcg gccacgccgg tgagacctac
     4081 ttctcgacca gcgacatcgg ctgggtcgtc ggccacagct acatcgtcta cgggccgctg
     4141 ctggccggca tggccaccat catgtacgag ggcctgccga tccggcccga cgcggcgatc
     4201 tggtggagcc tggtcgaaag gcacaaggtg acggcgatgt tcaccgcgcc gaccgcgatc
     4261 cgggtcctga agaagcagga tccggcgttc ctgcgccggc acgacctgtc gtcgttgcgc
     4321 gcccttttcc tcgccggcga gccgctcgac gagccgaccg cgtcgtggat cgccgccgcc
     4381 atcggcaagc cggtgatcga caactactgg cagaccgaga gcggatggcc gatcctcacg
     4441 gtggccaacg gcgtcgagaa gatgacgagc cggttcggca gccccggcgt gccgatgtac
     4501 ggattcgacg tgcgcctggt cgacgagagc accggcaagg acatcgtcga gcccgatcgc
     4561 aagggcgtcg tcgtcatcga cgggccgctg ccgcccgggt tcatgcagac cgtgtggcgc
     4621 gacgatgcgc gctacgtctc cacctactgg aagagcgtgc ccggccgcat ggtctattcg
     4681 accttcgatt ggggcatccg ggacgaagac ggctactact tcatcctcgg ccgcagcgac
     4741 gacgtgatca acgtcgccgg ccaccggctc ggcacg
//
LOCUS       DASRWS010000604         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1700746, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000604 DASRWS010000000
VERSION     DASRWS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700746"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFF72_11565"
     CDS             <1..659
                     /locus_tag="VFF72_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZV93848.1"
                     /translation="PLFDCESALAFSSTGRTMPLVAPLPSDHDPEIARLAKFFEQTLG
                     FAPNSVLTMMRRPAIARAFTDLNRAVMENHGRVTSELKRLVGHIASTTAGCRYCEAHT
                     IRAAERFGSSEGADRRRLDEVWSYATSELFTAAERAALDFARAAASVPNDVSPEVAAA
                     LREHWDEGEIVEITGVVALFGFLNRWNDSMATELEAAAAADGEQWLARRGWSRGKHAA
                     "
     gene            complement(699..944)
                     /locus_tag="VFF72_11570"
     CDS             complement(699..944)
                     /locus_tag="VFF72_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93849.1"
                     /translation="MDKQLRQLETFTARGSDGRTYSIRGYEHLGRVGALSSGVEPWEP
                     LGVVEYKLADGRPVRERKDGSFVVVGAGDLELHRQSH"
     gene            complement(1061..2296)
                     /locus_tag="VFF72_11575"
     CDS             complement(1061..2296)
                     /locus_tag="VFF72_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93850.1"
                     /translation="MTVLVYETDLGLHLNKTGGFTNLKWKSGEEMVSIELDKVSSASQ
                     TNPVLIAEVQEKSWPIVKKEAESLVKDLKKADSIVDKGKREAAADKALTEFRKDAGAK
                     LQRCAMDVFEVYIKDKGDYRLYLGKAGVRLLIDASSFAAAAVLTAASSWTGIGTAVGA
                     VSMARSAGSIIQQFGNLAADAETIQEQIRKNAEKLERQLTGGQLGNTVKQGLSAIINA
                     VTATEIENAFVTVEGLEKDFKLLESKVKGLHTNAASLGADLPKLLDAFEKIAPSQEIL
                     ADLVKLTGEKTSDYKKFTAELADVEKNIKKAVEGASDLMERVRTLQKWTDDRSDMVAD
                     MKKKYKEGAVKVTKVLAQFVTLGLAFYGGNWTDATSTIAGFKSSHALGAALAVANDGL
                     STVKEMGTELFDAFKKKKV"
     gene            complement(2435..3172)
                     /locus_tag="VFF72_11580"
     CDS             complement(2435..3172)
                     /locus_tag="VFF72_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZV93851.1"
                     /translation="MSRAWPLPALLAWSACWLVFWSLRSIGSALPLCAAVAMLVGGAL
                     AATASTPWRRLFVGLGFPLSCAASGLGSGLGAWPWLALLALLALAYPVGAWRDAPFFP
                     TPSGILAGLDRRAPLAAGARIVDLGCGLGDGLLELGRRYPQARLEGVEWSWPLRLAAA
                     LRCRFASVRRGDMWAEDWSPYAMVYLFQRPESMDRAWRKAVREMAPGSWLASLEFEVP
                     GRPADVILAGTGERHVFVYRMPASGAG"
     gene            complement(3187..3900)
                     /locus_tag="VFF72_11585"
     CDS             complement(3187..3900)
                     /locus_tag="VFF72_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HZV93852.1"
                     /translation="MYTAKGQLDLNSTLKQYSGLVRRLAHQMIAKLPANVEIDDLIQV
                     GMIGLTDALSRFDAAQGVQFETFATQRIRGAMLDELRGNDYLSRGTRKHQRSIESAVA
                     KLEQQFGRAPQESEIAREMGITLAEYQELLGKVRGTQLVYLEDMSGAEGDSDFLDRHV
                     VDEEANPLAQLQDHRMRAALIEAIKVLPEREQYVMSMYYEHDMNLKEIAAVLKVTESR
                     VCQLHSQSIARLRVKLRDY"
     gene            complement(3915..4715)
                     /locus_tag="VFF72_11590"
     CDS             complement(3915..4715)
                     /locus_tag="VFF72_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HZV93853.1"
                     /translation="MTLPTAPRNDQASGLRQMFGHTRVSFVPVVSNPHIGFGGVLLER
                     LCTAFAERQAKTLVVDAAERAGEAGEMAMVDLGQCIEPLSEHVSYLAARGLPIRFVDA
                     AGSTRDFLQRVAEAAPRCDVVLVHAGATELSRLFSQKRAGGAWSDLPCPIVLAEDHPD
                     SVTHAYAATKLLAHRAGLVVFDLLLGAAPQSPRAERIADKLGACADAFFGGALRRWAR
                     IDPAGDAGEAPGLDLRRLVAERLRGLRSADADASATPAASLRSTAAFP"
     gene            complement(4750..>5199)
                     /locus_tag="VFF72_11595"
     CDS             complement(4750..>5199)
                     /locus_tag="VFF72_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HZV93854.1"
                     /translation="ELLGAKKMVLIDTAGMAQRDTRTRELLEMLSHRSVQRLLVVNAA
                     SQGETIEDVLVAYRAASCAGVVLSKLDEAVKLGPALDAAIRHKLKIVGVANGQRVPED
                     WHHLSASALVQRALRGGGSSAWRFDAGEMNLVFASPSGMSPRAVVAA"
BASE COUNT          787 a         1769 c         1826 g          817 t
ORIGIN      
        1 tccccctctt cgactgcgaa tccgctctcg ccttttcttc gacaggacgc accatgccac
       61 tcgtcgctcc actgccctcc gaccacgacc cggaaatcgc ccgcctggcg aagttcttcg
      121 agcagacgct cggcttcgcg ccgaacagcg tcctgacgat gatgcgccgg ccggcgattg
      181 cgcgcgcctt caccgacctc aatcgtgccg tgatggaaaa ccacggccgg gtgacgagcg
      241 agctgaagcg gctcgtcggc cacatcgcca gcacgactgc gggctgccgc tactgcgagg
      301 cgcacacgat tcgcgccgcc gagcgcttcg gcagcagcga aggcgccgac cggcgccgcc
      361 tcgacgaggt atggagctac gcgacgagcg agctgttcac ggccgccgaa cgcgccgccc
      421 tcgacttcgc gcgagccgcg gcgagcgtgc ccaacgatgt ctcgccggaa gtcgccgccg
      481 cgttgcgaga gcactgggac gaaggcgaga tcgtcgagat caccggcgtc gtcgccctgt
      541 tcggatttct caatcgctgg aacgactcga tggcgactga actcgaggcc gccgcggcgg
      601 ccgatggcga gcagtggctc gcgcgccgcg gctggagccg cggcaagcac gcggcctgaa
      661 caatcgccgg cgcgccggcg cgccgcgacg ggttcgcgtc aatgggattg gcggtgcagt
      721 tcgagatcgc ccgcgccgac gacgacgaac gagccgtcct tgcgctcgcg taccggccgg
      781 ccatcggcga gcttgtattc gacgacaccg aggggctccc acggctcgac gccgctgctc
      841 aacgcgccga cgcggccgag atgctcgtag ccgcggatcg agtaggtgcg cccgtcgctg
      901 ccgcgcgcgg tgaaagtctc cagttgtctg agctgcttgt ccatgggtcc gaccctttcg
      961 cggcgtggac gaaccactct acgcgctcgt cgagcccatc gtcgtctcgc ggtctatcgc
     1021 tggacaagcg gccttgcacc gcgcattgcg cgcgcttgcg ctagaccttc ttcttcttga
     1081 aggcgtcgaa gagctcggtt cccatttcct tgacggtgct cagcccgtcg ttggcaaccg
     1141 ccagggcggc gcccagcgcg tgcgagctct tgaagccggc gatcgtgctg gtggcgtcgg
     1201 tccagttgcc gccgtagaac gccaggccga gggtgacgaa ctgggccagg accttggtca
     1261 ccttgacggc gccttccttg tacttcttct tcatgtcggc caccatgtcg gagcgatcgt
     1321 cggtccactt ctgcagcgtc ctgacacgct ccatcaggtc cgacgcgccc tcgacggcct
     1381 tcttgatgtt cttctcgacg tcggcgagct cggccgtgaa cttcttgtag tcgctggtct
     1441 tctcgccggt cagcttgacg aggtcggcca ggatctcctg gctcggcgcg atcttctcga
     1501 aggcgtcgag cagcttgggc aggtcggcgc cgagcgacgc ggcgttcgta tgcaggccct
     1561 tgaccttgct ttcgagaagc ttgaagtcct tctcgaggcc ctcgaccgtg acgaaggcgt
     1621 tctcgatctc cgtcgccgtg acggcgttga tgatcgccga gagtccctgc ttcacggtgt
     1681 tgccgagctg cccgccggtc agttgccgct cgagcttctc ggcgttcttc cggatctgct
     1741 cctggatcgt ctcggcatcg gcggccaggt ttccgaactg ctggatgatc gagccggcgg
     1801 agcgcgccat cgagacggcg ccgaccgccg tgccgatccc ggtccagctc gatgccgcgg
     1861 tgagcacggc ggccgcggcg aacgacgacg cgtcgatcaa caggcgcacg ccggccttgc
     1921 cgaggtagag ccgatagtcg cccttgtcct tgatgtagac ctcgaagacg tccatcgcgc
     1981 agcgctgcag cttggcgccg gcgtccttgc ggaattcggt gagtgccttg tcggcggccg
     2041 cttcgcgctt gcccttgtcg acgatcgaat cggccttctt caggtccttg acgagcgact
     2101 cggcctcctt cttgacgatc ggccagctct tttcctgcac ctcggcgatg agcaccgggt
     2161 tcgtctgcga cgccgacgag accttgtcga gctcgatcga caccatctcc tcgcccgatt
     2221 tccacttcag gttggtgaag ccgccggtct tgttcaggtg caggccgaga tcggtttcgt
     2281 agacgaggac cgtcatgcgg gtgggcgcgt ggcgcgaagt ggcgggggcg aaaggctagg
     2341 acgacgcgcg atgaaagtcc attcgccacg gcgggcgccg ccgtgaagcg tgcccgactg
     2401 cacgatcggc cacgctcgcc cgggtgcgcg agggtcagcc ggccccgctc gccggcatcc
     2461 ggtagacgaa cacgtggcgc tcgccggtgc cggcgaggat gacgtcggcc ggccggccgg
     2521 gcacttcgaa ctcgaggctc gccagccagc tgccgggcgc catttcgcga acggccttgc
     2581 gccacgcccg gtccatgctt tcgggccgct ggaacagata gaccatcgcg tacggcgacc
     2641 agtcctcggc ccacatgtcg ccgcggcgca cgctggcgaa gcgacaacgc agcgccgcgg
     2701 caagacgcaa cggccagctc cattcgacgc cttccaggcg agcctgcgga tagcggcgac
     2761 ccagctcgag caggccgtcg ccgagaccgc agccgaggtc gacgatgcgc gcgccggcgg
     2821 cgagcggggc ccgccggtcg aggccggcga ggatgcccga gggggtcgga aagaacggcg
     2881 cgtcgcgcca ggcgccgacc ggataggcca gcgcgagaag tgcgagcagg gccagccagg
     2941 gccaggcacc gaggccgctg cccaggcccg acgcagcgca cgagagcgga aagccgaggc
     3001 ccacgaagag gcgtcgccac ggcgtcgatg ccgtggccgc aagggcgccg ccgacgagca
     3061 tcgcaaccgc ggcgcaaagc ggcagcgccg agccgatcga gcgcaacgac cagaagacga
     3121 gccagcacgc cgaccaggcg agcagcgccg gcagcggcca ggcccgggac aaggtcgagc
     3181 ccgcggtcag tagtcgcgca gcttgacgcg cagccgggcg atcgactggc tgtgcagctg
     3241 gcagacgcgc gactcggtga ccttcagcac cgcggcgatc tccttgagat tcatgtcgtg
     3301 ctcgtagtac atgctcatca cgtactgctc ccgctcggga aggaccttga tcgcctcgat
     3361 cagcgccgcg cgcatgcgat ggtcctgcag ctgcgccagc ggattggctt cttcgtcgac
     3421 gacgtggcgg tcgaggaaat cgctgtcgcc ctcggccccc gacatgtcct cgagatagac
     3481 gagctgggtg ccgcgcacct tgccgagcag ctcctggtat tcggccaggg tgatgcccat
     3541 ctccctggcg atctcgcttt cctgcggcgc ccggccgaac tgctgctcga gcttggcgac
     3601 ggccgactcg atcgagcgct ggtgcttgcg cgtgccgcgc gagagatagt cgttgccgcg
     3661 cagctcgtcg agcatggcgc cgcggatccg ctgcgtcgcg aaggtctcga actgcacgcc
     3721 ctgggcggcg tcgaagcggc tgagcgcgtc ggtgaggccg atcatgccga cctggatcag
     3781 gtcgtcgatc tcgacgttgg ccggcagctt ggcgatcatc tggtgcgcga ggcgacggac
     3841 caggccgctg tattgcttca gcgtcgaatt caggtccagc tggcctttgg cggtatacat
     3901 ggcagctccg gagatcatgg gaacgcggcg gtggaccgca gcgaggcggc cggggttgcg
     3961 gaagcatcgg cgtcggcgct gcgcaggccg cgcaggcgct cggcgacgag ccggcgaagg
     4021 tcgaggcccg gcgcctcgcc ggcgtcgccg gcgggatcga tgcgggccca gcggcgcagc
     4081 gcgccgccga agaaggcgtc ggcgcaggca ccgagcttgt cggcgatgcg ctcggcccga
     4141 ggcgactgcg gcgcggcgcc cagcagcagg tcgaagacga cgaggccggc gcggtgcgcg
     4201 agcagcttgg tcgccgcgta ggcgtgggtg acgctgtcgg gatggtcctc ggccagcacg
     4261 atcggacaag gcaggtcgct ccacgccccg ccggcgcgct tttgcgagaa gagccggctc
     4321 agctcggtcg cgccggcatg gacgaggacg acgtcgcatc gtggggccgc ctcggcgacg
     4381 cgctggagga agtcgcgggt cgagccggcg gcgtcgacga agcggatcgg caagccgcgc
     4441 gccgcgaggt acgagacgtg ttccgagagc ggctcaatgc actggccgag atcgaccatc
     4501 gccatctcgc ctgcctcgcc ggcgcgctcg gcggcgtcga cgacgagcgt cttcgcctgg
     4561 cgctcggcga atgcggtgca caggcgctcg agcagcacgc cgccaaagcc gatgtgcggg
     4621 ttggagacca ccggcacgaa cgacacccgg gtgtgcccga acatctggcg caggccggag
     4681 gcctggtcgt tgcggggagc ggtcggcagc gtcatgggcg ggtcgaattc ggggtgtcgt
     4741 gcgcggccgt catgcggcga cgacggcgcg cggcgacatg ccggacggcg acgcgaagac
     4801 gaggttcatc tcgccggcgt cgaaacgcca ggccgagctg ccgccgccgc gcaaagcgcg
     4861 ctgcaccagc gcgctcgccg acaggtgatg ccagtcctcc ggcacgcgct ggccgttggc
     4921 gacaccgacg atcttcagct tgtggcggat cgccgcgtcg agcgcggggc cgagcttgac
     4981 ggcctcgtcg agcttgctga ggacgacacc ggcacagctc gcggcgcggt aggcgaccag
     5041 cacgtcctcg atcgtctcgc cttgcgacgc ggcgttgacg acgagcagcc gctgcaccga
     5101 acgatgcgac agcatctcga gcagctcgcg ggtgcgggtg tcgcgctggg ccatgccggc
     5161 ggtatcgatc aagaccatct tcttggcgcc gagcagctc
//
LOCUS       DASRWS010000605         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_820034, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000605 DASRWS010000000
VERSION     DASRWS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820034"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2119
                     /locus_tag="VFF72_11600"
     CDS             <1..>2119
                     /locus_tag="VFF72_11600"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV93855.1"
                     /translation="YGPYYDCIESLSRGPSQALARLKPAPYVVLLDAALRVAGAVHFG
                     SDGAARLPASIARYERCRVPTGETWAHAEVAEESDGVSVVDIRLIDGSGAAIASIERL
                     RLASAASVGGWHSWLHATRWIEAPSPFTVVESLRDGLGDTSVLEAALNAAAGAYAREA
                     LASVTEAEVAARYKRLWQHLPRLAAIPQGASLPQGPESTLLSRCGTALADVLRGKTDP
                     MSLLFADGDAAGIYRDAPAYRAANQTMAALAAAVLPPTGRVKVLEVGAGTGGTTGTVL
                     ASLDAGRTDYWFTDLSPTLVAQARGKLAVERFAVFDLERPPAGQDIPAGAFDLVMAAN
                     VLHATADLGTTLDHLLETLAPQGRLLLLETAPPGRKNEPAGWIDLVFGLTEGWWRFTD
                     KTLRPDYPLLQLDGWVTLLQSKGLEVEALSLPGHSVLLARRAEPSRVHRDVGGGAADR
                     CAALLDTIGSLKPDELRLTLLTDGAIGPAVSDPAAATLWGMMRTLRLERPELELRCLD
                     APRGDWDRVLATERLTAEPEIAQAGGSRFVPNLTTAEDGGASPSLNADAWYLVTGAFG
                     GLGSFIAEWLVRHGARRLVLSGRAAQTTAWIEQMRETGIAIRLEACDLADAAALQGLI
                     ARLPTIGGIVHAAGTLDDATLAQAKAQQFRKVLAPKVDAALALDAAFPDVELFVLFSS
                     AVGLFGQVGQATHVAASLGLDALA"
BASE COUNT          303 a          754 c          718 g          344 t
ORIGIN      
        1 aatacggtcc ctactacgat tgcatcgagt cgctgtcgcg cggtccctca caggcactcg
       61 ccaggttgaa gcccgcgccc tacgttgtct tgctcgatgc ggccctgcgt gttgccggcg
      121 ctgtccactt cggatcggac ggtgccgcac ggctgccggc gtccatcgcc cgctacgagc
      181 gttgccgcgt gccaaccggc gagacctggg cgcatgccga ggtcgccgag gaaagcgacg
      241 gcgtcagcgt cgtcgacatc cgcctgatcg acggatcggg cgcggcgatc gcttctatcg
      301 aacggctccg actcgcgtcg gctgcctcgg tcggcggatg gcacagctgg ctccacgcca
      361 cgcgttggat cgaagcgcct tcgcccttca ccgtcgtcga gtcactgcgc gatggcctcg
      421 gcgatacttc ggtcctcgaa gccgcgctca acgcggccgc cggtgcctac gcacgcgaag
      481 cgctggcgag cgtgaccgag gctgaggttg ccgctcgtta caagcgtctg tggcagcacc
      541 tgccgcgact ggccgcgatt ccgcaaggag cgagcctgcc tcaagggccc gagagtacat
      601 tgctcagccg ctgcggcacg gcgctggcgg atgtgctgcg cggcaagacc gatccgatgt
      661 cgttgctgtt cgccgatggc gacgccgcgg gcatctatcg cgacgcgccc gcctatcgcg
      721 ccgccaacca gacgatggcg gcgctggcag ccgccgtcct gccgccgaca ggccgcgtca
      781 aggtgctcga ggtcggtgcc ggtacgggtg gcacgacggg cacagttctc gcgagcctcg
      841 atgccggccg caccgactac tggttcaccg atctcagccc gaccctggtc gcacaagcgc
      901 gcggcaagct cgcggtcgaa cggttcgccg tgttcgacct cgaacgaccg ccggcggggc
      961 aggacatacc ggcaggcgcc ttcgatctcg tgatggcggc gaacgtcctg cacgcgaccg
     1021 ccgacctcgg tacgacgctc gatcacctcc tggagaccct tgctccacag ggccggctgc
     1081 tgctgctgga aacggcacct cccggacgca agaacgaacc ggccggctgg atcgatctcg
     1141 tcttcggcct gaccgaggga tggtggcgct tcaccgacaa gacgttgcgg ccggactatc
     1201 cgctgttgca gctcgatggc tgggtgacgc tgctgcagtc gaagggcctc gaggtcgagg
     1261 cgctgtcgct acccggccat tcggtgctgc tggcgcggcg cgccgagccg tcgcgcgtgc
     1321 atcgtgacgt cggtggcggc gctgccgacc gttgcgccgc gctgctcgac acgatcggct
     1381 cgctgaagcc cgacgaactg cgcctcacct tgctgaccga cggcgcgatt gggcctgccg
     1441 tcagcgatcc ggccgccgca acgctgtggg gcatgatgcg gacgctccgg ctcgaacggc
     1501 ccgagctcga gctgcgctgc ctcgatgcgc cgcgcggcga ctgggatcgc gtgctcgcca
     1561 ccgagcggct gaccgccgag ccggagatcg cgcaggccgg cggctcacgc ttcgtgccca
     1621 acctgaccac cgccgaagac ggcggcgcct cgccgtcatt aaatgccgat gcctggtatc
     1681 tcgtgaccgg agccttcggc ggcctcggct ccttcatcgc cgagtggctg gtgcgccatg
     1741 gcgcgcgccg gctggtgttg agcggccgag ccgcacaaac gacggcgtgg atcgagcaga
     1801 tgcgcgagac gggcatcgcc atccgcctcg aagcctgcga tctcgccgac gctgcggcgc
     1861 tgcagggctt gatcgcgcgc cttccgacaa tcggcggcat cgtccatgcc gcgggaacgc
     1921 tcgacgacgc cacattggca caggcaaaag cgcagcagtt ccgcaaggtc ctggctccca
     1981 aggtcgatgc cgccctggcg ctcgatgcgg ccttcccgga cgtcgagctg ttcgtgctgt
     2041 tttcctcggc ggtcggcctg ttcggccagg tcggccaggc cacgcacgtc gccgccagtc
     2101 tcggactcga cgcactggc
//
LOCUS       DASRWS010000606         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_820194, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000606 DASRWS010000000
VERSION     DASRWS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820194"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFF72_11605"
     CDS             <1..424
                     /locus_tag="VFF72_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZV93856.1"
                     /translation="FVEVKIDEDFGMVHVTRVVSAVAAGLIVNPRTAENQVRGAVVWG
                     IGQALHEEAQFDHRIGRIMNHSLAEYHIATQADVGKIDVIFVHEDDSEVNPIGVKGVG
                     EIGIVGVAAAIANAVFHATGRRLRSLPMTPDKVLGLVG"
     gene            complement(489..1133)
                     /locus_tag="VFF72_11610"
     CDS             complement(489..1133)
                     /locus_tag="VFF72_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV93857.1"
                     /translation="MTETRAMIRIGIACAAPLVRAGLVAGLGARDDVEVMLAAASVAA
                     LRDAGWGGAEVVVVELADSTDATGDDGLGAEGPRVVVLAEAGDERVGEWLIDGCAVLP
                     KGVPIDVVAGAIQAVRAGLVATSAAFAAEALRYERTGEGRQGVARGERILTPRERQVL
                     VEMSHGLGNREIGGALGISAHTAKFHVAQIIAKLDAQSRAHAVAKALRAGLVDA"
     gene            complement(1135..2100)
                     /locus_tag="VFF72_11615"
     CDS             complement(1135..2100)
                     /locus_tag="VFF72_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZV93858.1"
                     /translation="MNSPLRNDAATAATAALVAHSQAIAAIVERLADSTLAVRGRRGV
                     LASAVVWRDGVVVTAAHVFRRAPAATTLVGEGGATYEATLAGIDSSTDVAVFRVVGGA
                     ALRAIEVGDAAGLRAGHLAIAVGRGGDGDPLASAGLVNRAGGPWQTWLGGTIDRLIRL
                     DGGVYDGLSGAPVADASGAVVGIATAALSRRYGIVVPASTVSRVVDALLAGGRVSRPW
                     LGIGAQPVSLPARSAEAGAAAVGPDSGLLVTALAPKGPADRAGLLIGDIVVGVAGSGA
                     ADLASLRTALAGFVGKSARLSLLRGGAAREIDVEVGEQPAAGRCG"
     gene            complement(2288..3196)
                     /locus_tag="VFF72_11620"
     CDS             complement(2288..3196)
                     /locus_tag="VFF72_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZV93859.1"
                     /translation="MRTPITGEFEGGSVFIDGDWRPLSEAKVSMFDWGFTRSDVGYDV
                     ATTWQGAFFRLDDHLDRFFASLDKLRMTIPYDRAGVRRILHGCVRAGGIRDGYVAMLC
                     TRGVPPRGARDPRLAENRFYAYALPYVWIADPEKQARGIDLHVSDRVRISPDSVDPKV
                     KNYHWLDLVMSLFDAYDRGADTSCVVDAAGNVTEGPGFNVFMVKDGVVRTADRGVLEG
                     ISRKTAIELCGTLGIALRVEPVPAADLAAADEVFITSSGGGVLPIAKVDGQPVGRGFP
                     GPVTKRLQDAYWALHDDPRHRDPVEY"
     gene            3324..4259
                     /locus_tag="VFF72_11625"
     CDS             3324..4259
                     /locus_tag="VFF72_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93860.1"
                     /translation="MLQARGLCKSYGSRAVVTDLSFSVAAGEIVGLLGPNGAGKSTTV
                     AMLCGLVAADRGEVLVGSGADREVMTEHSREAKRRIGVVPQELSIYENLGAAANLELF
                     GALYGLHGPLLRERVDAALRLVGLADRARDKPSTFSGGMKRRLNIAAALVHDPDILIL
                     DEPTVGVDPQSRNAIFDNLEELRRRGKALLYTTHYMEEAERLCDRIVIVDHGRVVASD
                     TLAGIERMLPPTLSLEIEVDGPVDLAALADLPGIGRTTQDEAVLRTTVADLGRDTPAL
                     LAALVAQHRRVSRIGSARAGLEDVFLSLTGRALRD"
     gene            4262..>4580
                     /locus_tag="VFF72_11630"
     CDS             4262..>4580
                     /locus_tag="VFF72_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HZV93861.1"
                     /translation="MATTSTFSALFALVRKDLVLYFSNRRALVMSIAAPIVIAAFFGS
                     LFGSTGGKTTRIPVALADLDGSALSRQVGAGLRDDAALDVRELPAASAVDAVRRGDVR
                     VAIV"
BASE COUNT          664 a         1687 c         1607 g          622 t
ORIGIN      
        1 gttcgtcgag gtgaagatcg acgaggactt cggcatggtg cacgtcaccc gtgtcgtcag
       61 cgcggtcgcc gccggcctca tcgtcaatcc gcgcacggcc gaaaaccagg tccgcggtgc
      121 cgtcgtctgg ggaatcggcc aggcgctgca cgaagaagcg cagttcgatc accggatcgg
      181 ccggatcatg aaccacagcc tggccgaata tcacatcgcg acgcaggccg acgtcggcaa
      241 gatcgacgtc atcttcgtgc acgaggacga ctccgaggtc aatccgatcg gcgtcaaggg
      301 cgtcggcgag atcggcatcg tcggcgtcgc cgcggcgatc gccaacgccg tcttccacgc
      361 caccggacgg cgcctgcgca gcctgccgat gacgcccgac aaggtgctcg gcctggtggg
      421 ctgagggtca tcgggcgtcg atgtcgccga tgccgcggcg acgctcgacg ccgcgtgcag
      481 gccggtcctc aggcgtcgac cagtccggcg cgcagcgcct tggccaccgc gtgcgcgcgc
      541 gattgcgcgt cgagcttggc gatgatctgg gcgacgtgga acttggcggt gtgcgctgaa
      601 atgccgaggg cgccgccgat ctcgcggttg cccagcccgt ggctcatctc gaccaggacc
      661 tgtcgctcgc gcggcgtcag gatgcgctcg ccgcgggcga cgccctggcg gccctcgccg
      721 gtgcgctcgt agcgcagcgc ctcggcagcg aacgccgcgg acgttgcgac gaggccggca
      781 cgcaccgcct ggatggcgcc ggccacgaca tcgatcggca ctccctttgg cagcaccgcg
      841 cagccgtcga tcagccactc gccgacccgc tcgtcgccgg cttcggcaag gacgacgaca
      901 cgcggtccct cggcgcccag gccgtcgtcg cccgtcgcgt cggtactgtc ggccagctcg
      961 acgacgacga cttcggcgcc gccccaaccg gcgtcgcgca gggccgcgac cgacgccgcg
     1021 gcgagcatca cttcgacgtc gtcgcgagcg cccagcccgg cgaccagccc ggcgcggacg
     1081 agaggcgcgg cgcaggcgat gccgatccgg atcatggcgc gcgtctccgt catgtcagcc
     1141 gcaacgaccg gcggccggct gctctccgac ctcgacgtcg atctcgcgcg ccgcgccgcc
     1201 gcgcaggagc gaaaggcgcg ccgatttgcc gacgaagccg gccagcgcgg tccgcaacga
     1261 cgccaggtcg gcggcgccgg agccggcgac accgacgacg atgtcgccga tcagcaatcc
     1321 ggcgcggtcg gcggggcctt tcggggcgag cgccgtcacg agcaggccgc tgtcggggcc
     1381 gaccgccgcg gcgccggcct cggccgagcg cgccggcaac gacaccggtt gcgcgccgat
     1441 gcccagccac ggtcgcgaga cgcggccgcc ggcgagcagc gcatcgacga cgcgcgacac
     1501 cgtcgacgcc ggcacgacga tgccgtagcg gcgtgacagc gcggcggtgg cgatgccgac
     1561 gacggcgccg ctcgcgtcgg ccaccggcgc tccggagagc ccgtcgtaga cgccgccgtc
     1621 gaggcggatc aaccggtcga tcgtgccgcc gagccaggtc tgccacgggc cgccggcgcg
     1681 attgacgaga ccggcactcg ccagcggatc gccgtcgccg ccgcggccga cggcgatggc
     1741 caggtggccg gcgcgcaagc ccgctgcgtc gccgacctcg atcgctctca gggccgcccc
     1801 gccgacgacg cgaaaaacgg cgacatcggt cgacgagtcg atgccggcga gcgtcgcctc
     1861 gtaggtcgcg ccgccttcac cgaccagggt cgtcgccgcc ggcgcgcgcc ggaagacgtg
     1921 cgccgccgtg acgacgacgc cgtcgcgcca gacgactgcc gaggccagga cgccgcgtcg
     1981 gccacgcacc gccagcgtgc tgtcggcgag gcgctcgacg atggcggcga tggcctgcga
     2041 gtgcgcgacc agggccgccg tcgcggcggt cgctgcatcg ttgcggaggg gcgaattcat
     2101 gacgagcctt cgatgtctgc aatgcggccg aaggtacgcc ggcctcggca gcctcgaatc
     2161 cgccgaacgg ccggcatcgg cccgaaggtg tccagatgag ggacgcagcc ggcacttgca
     2221 aggcttcgcc gccacgaccc ggctccacgc cgccgccggt cccgacgcgg cgcgcacgac
     2281 gccgcgctca gtactcgacc ggatcgcgat gccgcgggtc gtcgtgcagg gcccagtacg
     2341 cgtcctgcag ccgcttcgtc accgggcccg gaaagccgcg tccgaccggc tggccatcga
     2401 ccttcgcgat cggcagcacg ccaccgccgc tcgaggtgat gaagacctcg tcggcagcgg
     2461 cgaggtcggc ggccggcacc ggctcgacgc gcaacgcgat gccgagcgtg ccgcacagct
     2521 cgatcgccgt cttcctcgag attccctcga gcacgccgcg atcggcggtg cggacgacgc
     2581 cgtccttgac catgaagacg ttgaagcccg ggccctcggt gacgttgccc gccgcatcga
     2641 cgacgcagct ggtgtcggcg ccccggtcgt aggcgtcgaa gagcgacatc accaggtcga
     2701 gccagtggta gttcttcacc ttcgggtcga ccgaatcggg cgagatgcgc acccggtcgc
     2761 tgacgtgcag gtcgatgccg cgtgcctgct tttcggggtc ggcgatccac acgtagggca
     2821 gcgcataggc gtagaagcgg ttctcggcca gccgcggatc acgcgccccg cgcggcggga
     2881 cgccacgcgt gcagagcatg gcgacatagc cgtcgcggat gcctccggcc cgaacgcagc
     2941 cgtgcaggat ccgcctcacc ccggcccggt cgtaggggat cgtcatgcgc agcttgtcga
     3001 ggctggcgaa gaagcggtcg aggtgatcgt ccaggcgaaa gaacgccccc tgccacgtcg
     3061 tcgcgacgtc gtagccgacg tccgagcgcg tgaagcccca gtcgaacatc gagaccttgg
     3121 cctccgagag cggccgccag tcgccgtcga tgaagaccga gccgccctcg aattcaccgg
     3181 tgatcggcgt gcgcatgctg tcctttcccg cggctggcgc cgcgcctggg ttgctgacgg
     3241 tgccccggcg tcgattcgtc gcatctcggc atccttcgcc ggccgcgccc gctagagtcg
     3301 cgccgatcga aaaaggagtc gagatgctgc aagcccgggg cctttgcaaa tcctacggca
     3361 gtcgcgccgt cgtcaccgac ctctcgttct cggtggctgc cggcgagatc gtcggcctgc
     3421 tcggaccgaa cggcgccggc aagtcgacga cggtggcgat gctgtgcgga ctcgtcgccg
     3481 ccgaccgcgg cgaggtgctc gtcggcagcg gcgccgaccg cgaggtgatg acggagcaca
     3541 gccgcgaggc gaagcggcgc atcggcgtcg tcccgcagga gctctcgatc tacgagaacc
     3601 tcggcgcggc ggccaacctc gagctgttcg gcgccctcta cggcctgcat ggcccgctgc
     3661 tgcgcgaacg cgtcgatgcc gcgctgcggc tggtgggcct ggccgatcgg gcccgcgaca
     3721 agccgtcgac cttcagcggc ggcatgaagc gtcgcctcaa catcgccgcc gcgctcgtcc
     3781 acgatcccga catcctgatc ctcgacgagc cgaccgtcgg cgtcgacccg caaagccgca
     3841 acgccatctt cgacaacctc gaggagctgc gccgccgcgg caaggcgctg ctctacacga
     3901 cgcactacat ggaagaggcc gagcggttgt gcgaccgcat cgtcatcgtc gaccacggca
     3961 gggtggtcgc gagcgacacc cttgccggca tcgaaaggat gctgccgccg acgctctcgc
     4021 tcgagatcga ggtcgacggg ccggtcgatc tcgcggcgct ggccgacctg cccggcatcg
     4081 gccgcacgac gcaggacgag gccgtcctgc gcacgaccgt tgccgacctg ggccgcgaca
     4141 cgccggcctt gctggccgcg ctggtcgcgc aacaccggcg cgtgtcgagg atcggctcgg
     4201 cgcgcgccgg cctcgaggac gtcttcctgt cgctgaccgg ccgcgcgctg agggactgac
     4261 gatggccacc acctcgacct tctcggccct ctttgcgctg gttcgcaagg acctcgtcct
     4321 ctacttctcg aaccggcgcg cgctcgtcat gtcgatcgcc gcgccgatcg tcatcgccgc
     4381 cttcttcggc tcgctcttcg gctcgaccgg aggcaagacg acacgcatcc cggtggccct
     4441 ggccgacctc gacggcagcg cgctgtcgag gcaggtgggc gccggcttgc gcgacgacgc
     4501 ggcgctcgat gtgcgcgaat tgcctgccgc ctcggccgtc gatgccgtgc ggcgcggcga
     4561 cgttcgcgtc gccatcgtcg
//
LOCUS       DASRWS010000607         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_245625, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000607 DASRWS010000000
VERSION     DASRWS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245625"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFF72_11635"
     CDS             <1..381
                     /locus_tag="VFF72_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93862.1"
                     /translation="LDFARLDHIDSPVAVEADSNIWVYVFIVVCAAIFWQAGPWPGVA
                     AVVVSLTLYLTLGRRYVRRRLASRVHERALADGEVWRRLWRFGGVTLVDASTADGEIA
                     RCAAPDGNWMALVRRLATCSDEDC"
     gene            565..1881
                     /locus_tag="VFF72_11640"
     CDS             565..1881
                     /locus_tag="VFF72_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZV93863.1"
                     /translation="MRRRAKTRPTSARRQSAAQPLPSLARRLVRDTDGATAVVAALIS
                     TALIGFVGLGSETGLWYYTHRSMQAAADSSALGAAAALQAGDTAGYPAEARAAAAKYG
                     FVPGAAGVTVAVNRPPSSGNFQGNLSTVEVIIQQPQTALFSALFLASAPTISARAVAL
                     QGKTGNDCVLALDAGASASTFGNGTTNVNLVGCSLAVNSNSGSALNLVGGATLSADSA
                     SIVGGYVDSGNGSITTTHGITTGAAAVADPYANVAVPAYSGCDSLSLPPHAATSVSGG
                     AVFCNGVTLNAGMTLNLSDGIYVIDRGALTVNGGATLNLTNATVVLTSSSGTNYATVD
                     IHGGATINATAPSSGETAGLAFFQDRNAPKGGTNNFSGGTTQNITGAIYLPNQIVNFA
                     GGTQTGNGCLQIVADEIDFRGNATLETHCTGTGVAAIGGSASKLVE"
     gene            1893..>2256
                     /locus_tag="VFF72_11645"
     CDS             1893..>2256
                     /locus_tag="VFF72_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZV93864.1"
                     /translation="MTIDMIRPGIVLAALRRNSGNAAIEFVLIAPLLISALVAVTDLG
                     IALYERMEIRNAAQAGAQYAIAKGWDSGAIENAVAGASTLSGISATPAPTQSCGCVSG
                     TAIVAAACGSLCPGGVAAG"
BASE COUNT          370 a          774 c          732 g          380 t
ORIGIN      
        1 ctcgacttcg cccgcctcga ccacatcgac agcccggtcg ccgtcgaggc cgacagcaat
       61 atctgggtct acgtcttcat cgtcgtctgc gcggcgatct tctggcaagc cgggccatgg
      121 ccgggcgtcg ccgcggtcgt cgtcagcctg acgctctacc tgaccctcgg gcggcgctat
      181 gtgcgccgcc gcctggccag ccgcgtccac gagcgcgccc tcgccgacgg cgaggtgtgg
      241 cgccggctgt ggcgcttcgg cggcgtcacg cttgtcgacg cgtcgacggc agacggggaa
      301 atcgcgcgct gcgccgcccc cgacggtaat tggatggcgc tggtccgccg cctcgctact
      361 tgctcagacg aagattgttg aggtcgttgg cgatcgctct gctcgcgagc gcgtgcccaa
      421 tgaagaccaa ttgtgaattt aacaacacta aatttttcct attattcaag gtgataccgc
      481 tcactggtat ggccgatccg cattattctg acatgacgtt ctgattactg tgaatttcag
      541 gcgagaagcg aggggagagc gggcgtgcgt cgtcgagcga aaacgcggcc tacatcagcg
      601 cggcgtcagt cggcggctca accgctgccg tcgctcgcga ggcggctcgt tcgtgacacg
      661 gatggcgcga cggccgtcgt cgccgctctg atttccaccg cgctgatcgg gtttgtcggt
      721 ctcggttcgg agaccggcct gtggtactac acgcaccgct ccatgcaggc agccgccgat
      781 tcgagcgccc tcggcgcggc ggcggcgctg caggccggcg ataccgccgg ctatcctgcg
      841 gaagctaggg cggcggcggc gaaatatggc ttcgttcccg gtgccgccgg cgtcaccgtc
      901 gccgtcaaca ggccgccgag cagcggcaac ttccagggca acctctcgac cgtcgaagtc
      961 atcattcagc agccccagac ggcgctgttc tccgccctgt tcctggcttc ggcaccgacc
     1021 atctcggcgc gcgccgtggc ccttcagggc aagaccggga acgactgcgt cctcgcgctc
     1081 gatgccggcg ccagcgcctc gacattcggc aacggcacga ccaacgtcaa tctcgtcggc
     1141 tgcagcctgg cggtcaattc gaacagcggc agcgccctca acctggtcgg cggcgccacg
     1201 ctctccgccg attccgcgtc gatcgtcggc ggctatgtcg acagcggcaa tgggtcgatc
     1261 acgacgacgc acgggatcac gaccggggcc gcggccgtcg ctgatcccta tgccaatgtg
     1321 gctgtcccgg catattcggg gtgcgacagc ctgtcgctcc ccccgcatgc ggcgacgtcg
     1381 gtctcgggcg gcgccgtctt ttgcaacggc gtcaccctca atgccggaat gaccctgaac
     1441 ttgagcgacg gcatctatgt catcgatcgc ggcgccctga cggtcaatgg cggtgccacc
     1501 ctcaatctga ccaacgcgac cgtcgtgctg accagcagct cgggaacgaa ctatgcgacg
     1561 gtcgacattc acggcggcgc caccatcaac gccacggcac caagctcggg cgagactgcc
     1621 gggctggcct ttttccagga tcgcaacgcg ccgaagggcg gtaccaacaa tttcagcggc
     1681 ggcaccaccc agaacatcac cggcgcgatc tacttgccga atcagatcgt caatttcgcc
     1741 ggcggcacgc agaccggcaa tggctgcctg cagatcgtcg ccgacgaaat cgacttcagg
     1801 ggcaacgcga ccctggagac acattgcacc ggcactggcg tcgccgcgat cggcggcagc
     1861 gcgagcaagc tggtcgagta gggaccgcgc tcatgacgat cgacatgatc cgccccggca
     1921 tagttctcgc ggcgttgcgg cgcaactccg ggaacgccgc catcgagttc gtcctgatcg
     1981 cgccgctgct gatctcggcg ctggtggcgg tgaccgatct cgggatcgcc ctctacgagc
     2041 gcatggagat ccgcaacgcg gcccaggccg gcgcgcaata cgccatcgcc aagggatggg
     2101 atagcggcgc gatcgagaac gccgtggcgg gcgcgagcac cctgagcggc atcagcgcga
     2161 cgccagcacc gacgcagagc tgcggttgcg tcagcggcac ggctatcgtg gcggcagcct
     2221 gcggctcgct gtgccccggc ggcgtggcgg cgggga
//
LOCUS       DASRWS010000608         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_245760, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000608 DASRWS010000000
VERSION     DASRWS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245760"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFF72_11650"
     CDS             <1..135
                     /locus_tag="VFF72_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HZV93865.1"
                     /translation="PHGHALEPGHIVLAGSFTRVVFAQKGDTLHADFGPLGGLAIQFV
                     "
     gene            146..916
                     /locus_tag="VFF72_11655"
     CDS             146..916
                     /locus_tag="VFF72_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HZV93866.1"
                     /translation="MSVEFQKNAFKAALAAGQLQIGLWSSLCSNIAADILSDSGFDWI
                     LLDTEHSPNEIPDLVGQLQAIRGGSATPIIRPAWNDAVLAKRALDIGAQTLLFPYVQN
                     VEEAKRAVASTRYPPHGVRGVSVAARASRYGRTPGYLGKANSEICVLVQVETREAMNE
                     LEAIARVDGIDGVFIGPSDLAASLGHLGNPAHAEVQTAIADAGRRLKKLGKPAGILTS
                     NEDEARRYIEWGYLFVAVGSDVGLLARNADTLAKKFKG"
     gene            1229..>2115
                     /locus_tag="VFF72_11660"
     CDS             1229..>2115
                     /locus_tag="VFF72_11660"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HZV93867.1"
                     /translation="MRAFSRFGRVNVLLGSVALLALATGAGMRAVADETCQSPYMARI
                     TGDEDFVYVWTLGVDGMGDGSDKLVTVDVRKGSPTFGKVINSASVGGQHEAHHGGFTD
                     DRRQFWAAGLSDSKIFIFDVASDPANPKLVKTIDDFVEKSGGAAGPHGAYALPGRMLM
                     PSLSNKDKTGHSALVEYSNDGEYIATHWLPAGSDTMGAKVESVADGYGYDARVLPRKN
                     VLLTSSFTGIENYMRPLGEVVSDPEAMKKFGNTVILWDFHARQPKTVLHVPGAPLEVR
                     WAWGPNNNYAFTSSALTSK"
BASE COUNT          397 a          654 c          687 g          377 t
ORIGIN      
        1 ccgcacggtc acgcgctcga gcccgggcat atcgtgctcg ccggctcgtt cacgcgcgtt
       61 gtgttcgcgc aaaaaggcga tacgctgcat gcggacttcg ggccgctcgg gggcctagcc
      121 attcagttcg tgtgaggagc gcgcgatgag cgtcgaattc cagaagaacg cgttcaaggc
      181 cgcgctggcc gccggccaat tgcagatcgg actgtggtcg agcctgtgca gcaacatcgc
      241 cgccgacatc ctgtcggact ccggcttcga ctggatcctg ctcgacacgg agcattcgcc
      301 gaacgaaatt cccgatctcg tcggccagtt gcaggcgatc cgcggcggca gcgcgacgcc
      361 gatcatccgg cccgcctgga acgacgcggt gctggccaaa cgcgcgctcg acatcggcgc
      421 gcagacgctg ctctttccct atgtgcagaa cgtcgaggag gccaagcgtg cggtcgcctc
      481 gacgcgctat cctccgcatg gcgtgcgcgg cgtctcggtt gcggcgcgcg cgagccgcta
      541 cggccgcacg ccgggctatc tcggtaaggc gaattcggaa atttgcgtgc tggtgcaggt
      601 cgagacgcgc gaggcgatga acgagttgga agcgatcgcg cgtgtcgacg gcatcgacgg
      661 cgtgttcatc gggccgtccg atctcgcggc ctcgctcggc catctcggca atccggcgca
      721 cgccgaagta cagacggcaa tcgcggatgc cggaaggcgg ctgaagaagc tcggcaagcc
      781 cgccggcatt ctcacgtcca acgaggacga ggcgcgccgt tatatcgagt ggggctatct
      841 gttcgtcgcg gtcggctccg acgtcggcct gctcgcgcgc aacgccgaca cgcttgccaa
      901 gaaatttaaa ggttaaattc gcccgctcct gcaagcggct ggacgtaaac gaagccgcga
      961 tttaggcggc ttcgatgcgt cgcgcattga ccgacgcagc ctgccgggct tacgctttgt
     1021 agatcgacga tcacgcctga tgcctgcggc gaatgccgaa gaaggcgaag gccgatcggg
     1081 acgagggact attcgcttcc gctccttcct gtgaaggatg cacaggctcc gttccctcat
     1141 cgacgaagac ctgacaatcg aatctgggaa ttgagcggag gcgctatcgg cgccgcaagc
     1201 aaggcgccgc agccgcaggg aggcggacat gcgagcgttc tcgagattcg gccgcgtcaa
     1261 tgtgcttctc ggaagtgtcg cacttctcgc gctcgcgacg ggcgcaggca tgcgggccgt
     1321 tgccgacgag acatgccagt cgccttatat ggcgcgaatt acgggcgatg aggatttcgt
     1381 ctacgtctgg actctcggcg tcgatggaat gggcgacggc tcggacaagc ttgtcaccgt
     1441 cgacgtgcgc aaaggctctc cgactttcgg caaggtgatc aattcggctt ctgtcggcgg
     1501 ccagcacgaa gcgcatcatg gcggcttcac tgacgaccgc cgccaattct gggcggccgg
     1561 gctttccgac agcaagatct tcatctttga cgtcgcgagc gatcccgcca atccaaaact
     1621 cgtcaagacg atcgacgact tcgtcgagaa aagtggtggc gcggcgggcc cacacggcgc
     1681 ctatgccttg ccggggcgaa tgctgatgcc gtcgctgtcg aacaaggata agacgggcca
     1741 ttcggctctc gtcgaatact cgaacgacgg agaatacatc gccacgcact ggctgccggc
     1801 cggaagcgac acgatgggag cgaaagtcga atccgttgcg gacggctacg gctacgatgc
     1861 ccgcgtgctg ccgcgcaaga atgtgctgct gacctcctcc ttcaccggta tcgagaacta
     1921 catgcgcccg cttggagagg tcgtgagcga tcccgaggcg atgaaaaaat tcggcaacac
     1981 ggtgatcttg tgggatttcc acgcgcgtca accgaagacc gtgctgcacg tgcccggagc
     2041 gccgctcgag gtgcgctggg cgtgggggcc taacaacaac tatgcgttca cgtcgtccgc
     2101 gctgacgtcg aaaat
//
LOCUS       DASRWS010000609         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3687967, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000609 DASRWS010000000
VERSION     DASRWS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3687967"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1333)
                     /locus_tag="VFF72_11665"
     CDS             complement(47..1333)
                     /locus_tag="VFF72_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZV93868.1"
                     /translation="MSLFNGTRAVGARPRRAALWLCIALAAPWLAGCGPKASSSAGGA
                     GAAPPPPEVGVITVKTQPVGLITELPGRLEASRVAQVRARVAGIVEKRLFTEGSDVKA
                     GQPLFRIDSAPYRATVASAQAALARAQAGVTQATAQAERYKPLLAANAISKQDYINAV
                     AAQKTAEADVAAARATLQSGQINLGYATVTAPISGRIGRALVTEGALVGQGEATPLAV
                     VQQIDPMYVNFTQSTTDVLKLRRAIASGKFKRADGRGDAASVRIILEDGSEYPQPARL
                     LFTDLTVDQTSGQVTLRAEVPNPQGLLLPGMYVRVRLEQAQTPDGILVPQQAVQRGST
                     GDSVMVVGADGKVSPRQVKVGSAKDGQWIILDGLKPGEQVMVDGFQKLQMMPPGTAVK
                     AVPWKAAAAAPAGAASGSAPAASAASAPASASAAKS"
     gene            complement(1599..2183)
                     /gene="metW"
                     /locus_tag="VFF72_11670"
     CDS             complement(1599..2183)
                     /gene="metW"
                     /locus_tag="VFF72_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZV93869.1"
                     /translation="MSDRKDIELISGLVPKGSRVLDLGCGDGELLALLSRERGCSGYG
                     IEIDDENVLACHRRGVNVIQLNLEEGLALFEDRSFDVVLQLDTLQHLKNTERMLRETA
                     RVGRVGIVSFPNFAHWPNRLHVAAGRMPVTRVLPYQWYDTPNIRVGTYADFEVLARKD
                     GLTILDAFGIQAGRVVRTWPNLRASVAVFKFQRA"
     gene            complement(2275..>2850)
                     /gene="metX"
                     /locus_tag="VFF72_11675"
     CDS             complement(2275..>2850)
                     /gene="metX"
                     /locus_tag="VFF72_11675"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561916.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZV93870.1"
                     /translation="HFYEHGLVPKRGLRIARMIGHITYLSDDVMNEKFGRQLRDDLRA
                     AAGQSPGTAAASGSVGAYKYSTQDIEFQIESYLRHQGDKFSEYFDANTYLLITRALDY
                     FDPARAHGGDLAKTFAAARCRFQIVSFSTDWRFAPARSREIVKALVDNRLDVSYAEID
                     APHGHDAFLLDDARYHGVLRACFANLAKELS"
BASE COUNT          420 a          992 c         1005 g          433 t
ORIGIN      
        1 gaagatcggt cggtcgataa agaacttggc catgcgggcg cgctccttac gacttcgccg
       61 ccgaggccga agccggcgcg gaagcggccg acgcggccgg tgcggaaccc gaggcggcgc
      121 cggcgggcgc ggcggccgcg gccttccacg gcaccgcctt caccgccgtg ccgggcggca
      181 tcatctgcag cttctggaag ccgtcgacca tgacctgctc gccgggcttc agcccatcga
      241 ggatgatcca ctgaccgtcc ttggccgagc cgaccttgac ctggcgcggc gacaccttgc
      301 cgtcggcgcc gacgaccatg acgctgtctc ccgtcgagcc gcgttgcacc gcctgctgcg
      361 gcacgaggat gccgtcgggc gtttgcgcct gctcgagtcg aacgcgcacg tacatccccg
      421 gcagcaacaa gccctgcgga ttcggcacct cggcccgcaa ggtgacctgc cccgacgtct
      481 ggtcgacggt gagatcggtg aaaagcagtc gggccggctg cggatactcg ctgccgtcct
      541 cgaggatgat gcgcacgctg gccgcgtcgc cgcggccatc ggcacgcttg aacttgccac
      601 tcgcgatggc acgcctgagc ttgagcacat cggtcgtcga ctgcgtgaag ttcacgtaca
      661 tcgggtcgat ctgctggacg accgcgagtg gcgtcgcttc gccctggccg accagcgcgc
      721 cttcggtgac gagggcccgg ccgatgcggc ccgagatcgg cgccgtcacg gtggcgtagc
      781 cgagattgat ctggccactc tgcagcgtcg ccctggcggc ggcgacgtcg gcctcggccg
      841 tcttctgcgc ggcaacggcg ttgatgtagt cctgcttgct gatcgcgttc gcggccagca
      901 gcggcttgta tcgctcggcc tgcgccgtcg cctgcgtcac gccggcctgg gcccgtgcca
      961 gcgccgcctg ggcgctcgcg acggtggccc ggtagggcgc cgaatcgata cgaaagagcg
     1021 gctggccggc cttgacgtcg ctgccctcgg tgaagaggcg cttctcgacg atgccggcga
     1081 ctcgcgcccg cacctgagcg acgcgcgacg cctcgaggcg tcccggcagc tcggtgatga
     1141 ggccgacggg ctgcgtcttg accgtgatca cgcccacttc gggcggcggc ggcgccgcgc
     1201 ccgcgccccc tgcggaagac gacgccttcg gaccgcaccc cgcgagccac ggcgcggcga
     1261 gggcgatgca caaccagagc gccgcgcggc gcgggcgagc cccgacggcg cgagtgccat
     1321 tgaacagcga catgtcagtt cccgacgccg gggaccgcat cgcgtcccga cggttgatcg
     1381 caaaagctgc gaagaatagc cgaggcaggg cccgggcgtt cacgacgtgc cgggcacggg
     1441 cccgatcgca acctctcgcg ccttgtcggc ggccgaggcg ccattcgcgt tcccgaggcg
     1501 gcatccaccg gccccggcgc gggcgcgccc agcgaggtcg cgtcgatcgc cgcgaacggg
     1561 aaccgagcga cgcgcccacg gcgcccggcc ttgcgagttc acgctcgctg gaacttgaac
     1621 acggcgacac tggcgcgcag gttcggccac gtgcgcacga cccggcccgc ctggatgccg
     1681 aaggcgtcga ggatcgtcag gccgtccttt ctcgccagga cctcgaagtc ggcataggtg
     1741 ccgacgcgaa tgttgggcgt gtcgtaccac tgatagggca gcacgcgcgt caccggcatc
     1801 cggccggcgg cgacgtgcag gcggttcggc cagtgcgcga agttcggaaa gctgacgatg
     1861 ccgacacggc cgacccgcgc cgtctcgcga agcatgcgct cggtgttctt cagatgctgc
     1921 agtgtgtcga gctggagcac gacgtcgaag ctgcgatcct cgaacagcgc caggccctct
     1981 tcgaggttga gctggatcac gttgacgcca cgccgatggc aggccagcac gttctcgtcg
     2041 tcgatctcga tgccgtagcc gctgcagccg cgctcgcgcg aaagaagcgc cagcagctcg
     2101 ccgtcgccgc aacccaggtc gaggacgcgc gagcctttcg gaacgaggcc ggagatcagc
     2161 tcgatgtcct tgcgatcgct cacgacggaa cccccacgct ggccggtgcg cgacggcgct
     2221 gcccgcggcg cgggcgccgc ggtcctcggg gcggcgcggc ggccggccgg cccttcacga
     2281 cagctccttc gcgaggttgg cgaagcacgc gcgaaggacg ccgtggtagc gcgcatcgtc
     2341 gagaaggaaa gcgtcgtggc cgtgcggcgc atcgatctcg gcatagctga cgtcgagccg
     2401 gttgtcgacg agcgccttga cgatctcgcg cgagcgggcc ggagcgaatc gccagtcggt
     2461 gctgaagctg acgatctgga aacggcagcg ggccgccgcg aaggtcttcg ccaggtcgcc
     2521 gccatgcgcg cgcgccgggt cgaagtagtc gagggcacgg gtgatcagca ggtaggtgtt
     2581 ggcgtcgaag tactcgctga acttgtcgcc ctggtggcgc aggtagctct cgatctggaa
     2641 ctcgatgtcc tgcgtgctgt atttgtacgc ccccacgctc cccgacgcgg cggccgtgcc
     2701 tggggactgc ccggccgcgg cgcgcaagtc gtctcgcaac tgtctgccga acttctcgtt
     2761 catcacgtcg tccgacaggt acgtgatgtg gccgatcatg cgtgcgatgc gcagcccccg
     2821 cttgggcacc aggccgtgct cgtagaagtg
//
LOCUS       DASRWS010000610         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2522858, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000610 DASRWS010000000
VERSION     DASRWS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522858"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /locus_tag="VFF72_11680"
     CDS             complement(<1..2235)
                     /locus_tag="VFF72_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HZV93871.1"
                     /translation="MSPRSPNARAATKHRKPPRSRRRPFRLAARILLGLVGTAWAILF
                     VGWLSLHWLILPHIEQWRAPIEARASRMLGAPVRIGAIEVRSSGWVPSIDLRDVRILD
                     AQQRVALSLAHVTAAFSTHSLLVLEPRFEQLLIESPTLDVRRDAAGHIRVAGFDFGSS
                     KMSADDGGAAADWFFKQHEFVIRNGTVRWIDEARQAPPLVLSGVDIVVRNGLRSHAMR
                     IDATPPVEWGDRFRVRGRFEQPLFARAGDWRRWQGTVYAELPRADVRELRQHVELPFE
                     LSEGDGALRGWFDISQGRPRAATVDVSLRAVTLRLDKTVEPLQFAQIEGRIGARKSGE
                     RTSVDVHGFGFVTGDGIRWPRGDLDVSWHQTEGGDVDGGDFAADRLDLGVMAGIASRV
                     PLGAALRNLLADLHPQGIITGLKTRWDGPLDAPVHYHVAGNLSGLSLSAHAAATADAI
                     GRPGLSNATIGLVADETGGQARIAVKDGILDVPGVFADPALPLARLDSHLTWTIDPGR
                     SAGEAPRITVKVDSAQFANADAQGDLTATWRTGAGSSSGMAHGDRYPGRLDLEGRLTN
                     AQAIRTVRYLPLGLPDSVRDYVGRAVRGGTIPSATFRVRGDLADFPFHGARSAGDSEF
                     HIAAKVDNLTFDYVPDRRTGSGRGSKDPAAATTGVDAAGRSAPRVAGATTASSRAGGR
                     AATPAAGADAVLPADLAASAAAPGIAVAGTSAPGGRTETWPPLTALTGELVIDRSTLE
                     VKNAR"
     gene            2368..5121
                     /gene="glnE"
                     /locus_tag="VFF72_11685"
     CDS             2368..5121
                     /gene="glnE"
                     /locus_tag="VFF72_11685"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HZV93872.1"
                     /translation="MGSPSVGRAEVNTPRASPRTGPADAAHSRFVQRVRRRYVDELPL
                     LAPGLPRRATILPLVEALRSAGRSLSAALRVARQLAIERLAVLDVEHDASLADVTRSM
                     TELAETTLDLALAQALVDQDARFGVPRNAAGERIDFWVIGMGKLGARELNVSSDIDLV
                     YVYEEEGTTDGAQSLSAHEYFSQVARSLYAYIGEVTDDGFVFRVDLALRPNGNSGPPV
                     VSLAMLDEYLQSHGREWERFAWLKSRVVAPLASVSSGRALALRSLVTSFVYRRYLDYG
                     VFEGLRQLHEKVRGEAQRRAAGRPERANDVKLSRGGIREIEFIVQLLLVVRGGQFPEI
                     RTRSTLKALDKLAAGGLMKRASAERLAAAYILLRRVEHRIQYLDDQQTHVLPTHDGDV
                     DWIASSLGPEVGGACGLIDRLGEAREFVAAEFDLLLNDGRSGGAGCRTCGTPLPPLDS
                     PELLERLPEELAARVRPLCDQPKIRLLRDESKVRLARLVSRAAQAVEKGRCTLQAAVL
                     FIDWLEPLLRRESYLALLAERPEVQNRLLRLLGLARWPMRYLMLHPGVIDELADEELL
                     HGRFDAKAYTRELEERHAGWERSSQAEEELLLDTLRRAHHAEVFRTLVRDVEGHLGVE
                     EVADDLSALADATLSCAMRWAWKHLKSAHRDEPQLAVIAYGKLGGKELGYGSDLDVVF
                     VHDDPETDADKAAEVYGAFVRKLIAWLTMRTAAGELFDIDTALRPNGNSGLLVTTMSS
                     FENYQAGRGSNTAWTWEHQALTRARWCAGLATLAPRFEAVRRGVLAAPRDVAALRDEV
                     RSMREKVRRAHGIKGDAFDVKHSPGGMMDVEFAVQTLVLAESRSYPQLLDDVGNIALL
                     RRAEAAGLLPAGVGAAAADAYRDLRRVQHQARLDEQPTQVDPATMSEQRSAVLALWRA
                     VFG"
BASE COUNT          740 a         1775 c         1859 g          756 t
ORIGIN      
        1 ccgtgcgttc ttcacctcga gcgtgcttcg atcgatcacg agctcgccgg taagcgcggt
       61 caggggcggc cacgtctcgg tgcgcccgcc gggcgcgctc gtgccggcga cggcgatgcc
      121 cggtgctgcc gctgaagccg cgaggtcggc aggtaagacg gcgtcggcac cggcggctgg
      181 ggtggccgcg cggccaccgg cccgggaaga cgcggtggtc gcaccggcca cgcgaggggc
      241 ggaccgaccg gctgcatcga cgccggtggt ggcggcggcc gggtccttcg agccgcggcc
      301 gctgcccgtc cggcgatcgg gcacgtagtc gaaggtcagg ttgtcgacct tggcggcgat
      361 gtgaaactcg ctgtcgccgg cgctcctggc gccatggaag ggaaagtcgg caaggtcgcc
      421 gcggacccga aacgtcgccg acggaatcgt gccgccacgc acggcccggc cgacatagtc
      481 gcgtaccgag tcgggcagcc cgagcggcag atagcggacg gtgcggatcg cctgggcatt
      541 ggtgagccgg ccttcgagat cgagccgtcc cggatagcgg tctccgtggg ccatgcccga
      601 gctgctgccg gcgcccgttc gccacgtggc ggtcagatcg ccttgcgcat cggcgttggc
      661 gaactgcgcg ctgtcgacct tgaccgtgat cctgggcgct tcgccggccg atcgccccgg
      721 gtcgatcgtc catgtcagat ggctgtcgag tcgggccagc ggcagcgccg ggtcggcgaa
      781 gacgccgggg acgtcgagga tcccgtcctt gacggcgatc cgcgcctggc cgccggtttc
      841 gtcggcgacg agcccgatcg tcgcgttgct gaggcccggc cggccaatgg cgtcggccgt
      901 cgccgcggca tgggcgctca gcgacaggcc gctcaggttg ccggcgacgt ggtagtgcac
      961 cggcgcgtcg agcgggccgt cccagcgcgt cttcaggccg gtgatgatcc cttgcggatg
     1021 caggtcggcg agcaggttgc gcaatgcggc gcccagcggc acgcgactgg cgatgccggc
     1081 catcacgccg aggtcgagac gatcggcggc gaagtcgccc ccgtcgacgt cgccgccttc
     1141 ggtctggtgc caggagacat cgaggtcgcc ccggggccag cggatgccgt cgccggtgac
     1201 gaatccgaag ccgtgcacgt cgaccgacgt ccgctcgcca ctctttctgg cgccgatccg
     1261 cccctcgatc tgcgcgaact gcagcggctc gacggtcttg tcgaggcgca gcgtgaccgc
     1321 gcgcagcgac acgtcgacgg tggcggcgcg cggccgaccc tggctgatgt cgaaccagcc
     1381 gcgcaatgcg ccgtcgcctt cgctcagctc gaacggcagc tcgacgtgct ggcgcaactc
     1441 gcgcacgtcg gcgcgcggca gctcggcgta gacggtgccc tgccagcgtc gccagtcgcc
     1501 ggcgcgcgcg aacagcggct gctcgaagcg tccgcgcact ctgaagcgat cgccccattc
     1561 gaccggtggc gtcgcgtcga tccgcatcgc gtgactgcgc aagccattgc gaacgacgat
     1621 gtcgacgccg gacaggacca gcggcggcgc ctggcgggct tcgtcgatcc agcggacggt
     1681 gccgttgcgg atcacgaact cgtgctgctt gaagaaccag tcggccgcgg cgccgccgtc
     1741 gtcggcgctc atcttgctgc tgccgaaatc gaagccggcg acgcggatgt ggccggccgc
     1801 gtcgcggcgc acgtcgaggg tcggcgactc gatcagcaac tgctcgaacc tcggctcgag
     1861 gacaagcagc gaatgcgtgg agaaagccgc ggtgacgtgg gccaggctga gcgcgacgcg
     1921 ttgctgggcg tcgagaatgc ggacgtcgcg cagatcgatc gacggcaccc agccgctcga
     1981 gcgaacctcg atggcgccga tccggaccgg cgcgccgagc atgcggctgg cgcgtgcttc
     2041 gatcggtgcg cgccactgct cgatgtgcgg cagaatcagc cagtgaagtg agagccaacc
     2101 tacgaagaga atggcccagg ccgtgccgac cagcccgagc aggatgcgag cggcgaggcg
     2161 aaaggggcga cgacgcgagc gaggcggctt gcggtgcttc gttgccgctc gtgcgttggg
     2221 agaccggggc gacatgaatg cagcgttgcg acgacccgcg ggttgccagg gtgccgttcg
     2281 gcccgccacg aggatgagac ggcggcgcgg actcctgcga cgccttgcgc cggtcggttc
     2341 aatctagcac agacatcgca cgacaccatg ggttcaccga gcgtcggccg tgccgaagtg
     2401 aacacaccgc gggcctcccc tcggaccggt ccggccgatg cggcacacag ccgcttcgtg
     2461 cagcgagtgc gccgccgcta tgtcgacgag ttgccgctgc tggcgcctgg cctgccgcga
     2521 cgcgcgacga tcctgccgct cgtcgaagcg ctccgcagcg cgggtcgcag cctgtccgca
     2581 gcactgcgcg tggcgcgcca gctggcgatc gagcggctgg cggtgctcga cgtcgagcac
     2641 gacgcgtcgc tggccgacgt gacgcgctcg atgaccgagc tcgccgagac gacgctcgac
     2701 ctcgcgctcg cccaggcgct ggttgaccag gacgcgcgct tcggggtgcc gcgcaacgcc
     2761 gccggcgagc ggatcgattt ctgggtcatc ggcatgggca agctcggcgc gcgcgagctc
     2821 aacgtctctt ccgacatcga cctcgtctat gtctacgagg aagagggcac gaccgacggc
     2881 gcgcagtcgc tgagcgcgca cgaatacttc tcgcaggtcg cgcgaagcct gtacgcgtat
     2941 atcggcgagg tcaccgacga cggcttcgtc ttcagggtcg acctggcgct gcggccgaac
     3001 ggcaactccg gtccgccggt ggtcagcctg gcgatgctcg acgaatacct gcagtcgcac
     3061 ggccgcgagt gggagcgctt cgcgtggctg aagagccgcg tcgtcgcgcc gctcgcgagc
     3121 gtctcgagcg gccgcgcgct ggcgctgcgg tcgctcgtga cctcgttcgt gtaccggcgc
     3181 tatctcgact acggcgtctt cgaaggcttg cgccagttgc acgagaaggt ccgcggcgag
     3241 gcgcagcgcc gcgccgcggg gcgtcccgag cgcgccaacg acgtcaagct gtcgcgcggc
     3301 ggcatccgcg agatcgagtt catcgtccag ctgctgctgg tcgtgcgtgg cggccagttc
     3361 ccggagatcc gcacccgctc gaccctgaag gcgctggaca agctcgccgc cggcggcctg
     3421 atgaagcggg ccagcgccga gcgtctggcc gccgcctaca tcctgctgcg tcgggtcgag
     3481 caccgcatcc agtatctcga cgatcagcag acgcacgtgt tgccgaccca cgacggcgac
     3541 gtcgactgga tcgcgtcgag cctcggtccg gaggtcggtg gtgcctgcgg cctgatcgac
     3601 cgcctcggcg aggcccgcga gttcgtcgcc gcggagttcg acctccttct caacgacggg
     3661 cgctcgggcg gcgccggctg ccgcacctgt ggaacgccgc tgccgccgct cgattcgccc
     3721 gagctgctgg aaaggcttcc ggaggaactg gccgcgcggg tgcgaccgct ttgcgatcaa
     3781 ccgaagatcc ggctgctgcg cgacgaaagc aaggtccgcc tggcgcgcct cgtgtcgcgg
     3841 gcggcccagg cggtcgagaa ggggcgttgc acgctgcagg cggcggtcct gttcatcgac
     3901 tggctcgagc cgctgttgcg acgcgagagc tatctggctt tgctcgccga gcgaccggag
     3961 gtgcagaacc gcctgctgcg tctgctcggc ctggctcgtt ggccgatgcg ctatctcatg
     4021 ctccatcccg gcgtcatcga cgagctggcc gacgaggagt tgctgcacgg ccgtttcgac
     4081 gccaaggcct acacgcgtga gctcgaggaa cgccacgcgg gctgggagcg ctccagtcag
     4141 gccgaggaag agctgctgct cgacaccctt cgccgcgccc atcatgccga ggtctttcgc
     4201 acgctggtgc gcgacgtcga aggccacctc ggtgtcgagg aggtggccga cgacctgtcg
     4261 gcgctcgccg acgcgacgct gtcgtgcgcg atgcgctggg cctggaagca cctgaagtcg
     4321 gcgcatcgcg acgagccgca gctcgcggtc atcgcctacg gcaagctcgg cggcaaggag
     4381 ctcggctacg gcagcgatct cgacgtcgtg ttcgtccacg acgatcccga gaccgacgcc
     4441 gacaaggcgg ccgaggtcta cggcgccttc gtgcgcaagc tgatcgcctg gctcaccatg
     4501 cgaaccgccg ccggcgagct cttcgacatc gacaccgcgt tgcgccccaa cggcaactcg
     4561 ggactcctcg tcacgacgat gagctcgttc gagaactacc aggcgggccg tggcagcaat
     4621 accgcatgga cctgggagca ccaggcgctg acccgggcgc gctggtgcgc cgggctggcg
     4681 acgctcgcgc cgcggttcga ggcggtgcga cgcggtgtcc tggccgcgcc gcgcgacgtc
     4741 gcggcgctgc gcgacgaggt tcgctcgatg cgcgagaagg tccggcgcgc ccatggcatc
     4801 aagggggatg ccttcgacgt caagcacagc ccgggcggca tgatggacgt cgaattcgcg
     4861 gtgcagacgc tcgtcctcgc cgagagccgc tcgtacccgc agctgctcga cgacgtcggc
     4921 aacatcgcgc tgctgcggcg tgccgaagcg gccggcctgc tcccggcagg cgtcggcgca
     4981 gccgccgccg atgcgtaccg cgatctgcgc cgcgtccagc accaggcccg cctcgacgag
     5041 caaccgacgc aggtcgaccc ggcgacgatg tccgaacaga gaagcgcggt cctcgccctc
     5101 tggcgtgcgg ttttcggctg acgccggcgg
//
LOCUS       DASRWS010000611         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3437360, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000611 DASRWS010000000
VERSION     DASRWS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437360"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="VFF72_11690"
     CDS             complement(<1..1627)
                     /locus_tag="VFF72_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZV93873.1"
                     /translation="MVVVRDGAAGLEVLLSRRAESIDHTSGAWVFPGGIVDADDREAH
                     ASCEGLDDRAASDRLGVAEGGLDYWVAAIRECFEESGLLFCYAADGRLVDLDGADATR
                     LAPWRRALHRRERSIGALCASENLRLAVDQLVYLSHWLTPLGRAKRYDTRFFIAAMPK
                     AQTALFDGTEMLEQMWVTPTEALARGQSLKLLTPTSRTLEAIAGFANVASVMAWARAP
                     RSVSLTMPRVGVGSEGMRPVLPDEPSWPELGRIDPQGHGHGSYEIVADRPVRLSSRVI
                     RVTAGNGSVMTGPGTNTYLVGGGTANEWAVIDPGPALDPHVDAIVAAAPGPIRKIFVT
                     HTHTDHSPATAALKARTGATVFGRLTNHRQWQDATFAADMTLSGGERIEIAPGTTLSA
                     IHTPGHASNHLCYLLEEEKTLFTGDHVMQQSTVVINPPDGDMRAYLDSLRSLLDADLE
                     WLAPGHGFLMAEPRRAMQSIIEHRLRREAKVVGAMRELGAAPADRLLPAVYADVPARL
                     HPMALRSLTAHLLKLRDDGVAEEDAGTWSLRPER"
     gene            1936..2100
                     /locus_tag="VFF72_11695"
     CDS             1936..2100
                     /locus_tag="VFF72_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HZV93874.1"
                     /translation="MLHYAVVFFVIALIAALFGFGGIAAGAAGIAKILFVVFLILAIV
                     SFLFGRNRGL"
BASE COUNT          358 a          765 c          775 g          373 t
ORIGIN      
        1 cccgctccgg ccgcagggac caggttccag cgtcttcttc agcgacgccg tcgtcgcgca
       61 gcttcagcag atgcgcggtc agcgagcgaa gcgccatcgg atgcaggcgg gcgggaacgt
      121 cggcatagac ggcaggcagc agccgatcgg ccggcgccgc gccaagttcg cgcatcgcgc
      181 cgacgacctt cgcttcgcgt cgcaggcggt gctcgatgat cgactgcatt gcccgccgcg
      241 gctcggccat caggaagcca tggcccggcg caagccactc gagatcggca tcgaggaggc
      301 tgcgcagcga gtcgagatag gcgcgcatgt cgccatcggg cggattgatc acgacggtgg
      361 actgctgcat cacatgatcg cccgtgaaca gcgtcttttc ttcctcgagc agatagcaga
      421 gatgattcga agcgtggccc ggcgtgtgaa tggccgacag cgtcgtcccg ggcgcgatct
      481 cgatgcgctc gccaccggat agcgtcatgt cggccgcgaa ggtcgcgtcc tgccactgcc
      541 gatgattcgt gagccggccg aagaccgtgg ctcccgttcg cgccttcagc gcggccgtcg
      601 ccggcgaatg atccgtgtgg gtatgcgtga cgaagatctt ccggatcggc cccggtgccg
      661 cggcgacgat cgcatcgacg tgcggatcga gggccgggcc gggatcgatg acggcccatt
      721 cgttggccgt gccgccgccg accagatagg tgttcgttcc cggcccggtc atcacgctgc
      781 cgttgccggc cgtgacgcga atgacgcggc tcgaaaggcg caccggccgg tcggcgacga
      841 tctcgtagga gccgtggccg tgaccttgcg gatcgatccg cccgagctcc ggccaggacg
      901 gctcgtcggg cagcaccggt cgcatgcctt cgctaccgac gcccacccgc ggcatcgtca
      961 gcgacacgct ccgcggcgct ctggcccatg ccatcaccga ggcgacgttc gcaaatccgg
     1021 cgatcgcctc cagcgtcctg gacgtgggag tgagcagctt cagcgactgg ccgcgcgcca
     1081 gcgcctcggt cggggtgacc cacatctgct cgagcatttc cgtgccgtcg aagagcgcgg
     1141 tctgcgcctt cggcatggcg gcgatgaaaa agcgggtgtc gtaccgtttg gcacgaccga
     1201 gcggcgtcag ccagtggctg agatagacga gctgatcgac ggccaggcgc aggttttcgc
     1261 tggcgcaaag cgcgccgatc gaccgctcgc gtcggtgcag tgcgcgacgc catggcgcga
     1321 gccgggtcgc gtcggcgccg tcgagatcga cgagacgacc gtcggcggca tagcaaaaaa
     1381 ggagacccga ctcctcgaag cactcgcgaa tcgctgccac ccagtagtcg aggcctcctt
     1441 cggcgacgcc cagtcgatcg ctcgccgcgc gatcgtcgag gccctcgcac gaggcatgcg
     1501 cttcacggtc gtcggcatcg acgatgccgc caggaaagac ccacgccccg ctcgtgtgat
     1561 cgatgctttc ggcgcgtcgc gacagcagga cttcgagccc cgccgccccg tcacgaacga
     1621 cgaccatcgt cgcggccagg cgaggcggtc gattcgaatc gtcgggggtc ggcggaggaa
     1681 acggtggcgc ggttggcagg gatgaagaca tggcggcgga caaggctcgc gaggggcgac
     1741 gcgacgacgc gtccagtgtg gcacggccgg cgctccgacg cgccggcgtc ggagccgtcc
     1801 gacagcgcgt ccctcagcct cccgacatcg tcgaagcccg ccagcctatc gctgggacga
     1861 cgagccgacc cgatgatgac tgcacccgcc aaggctctcg gcaaccgcag gcccggcgct
     1921 caaccaggag ttcacatgct tcactatgcc gtcgtatttt tcgtgatcgc actgatcgcg
     1981 gcgctgttcg gcttcggcgg cattgccgct ggcgccgccg gtatcgccaa gatccttttc
     2041 gtggtgttcc tcattctcgc gatcgtttcc ttcctgttcg gccgaaatcg cggtctgtag
     2101 acgggaaaat ccggacgacc ccgcgttcgc ccctcggggt ggacgcgggg tttcttcgtt
     2161 tggcgggacg gcggtgcgag cgccgttgtt cacgtcaaac ggatgcccgg attcgctcga
     2221 tcgtcgccat cgccgcggcg ggattgcgct ccttggcgcc gctgatgaag a
//
LOCUS       DASRWS010000612         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2524552, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000612 DASRWS010000000
VERSION     DASRWS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524552"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFF72_11700"
     CDS             <1..412
                     /locus_tag="VFF72_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV93875.1"
                     /translation="FARHWLDRVGLTRRADEPAGSLPYGDQRRLEIARAMCTRPLLLC
                     LDEPAAGLNARESDGLNELLFSIRAEEGISLLLIEHDMSVVMRISDHVVVLDYGKKIA
                     DGTTDEARNDPRVIAAYLGVPDEELPVIEREVGL"
     gene            421..1164
                     /locus_tag="VFF72_11705"
     CDS             421..1164
                     /locus_tag="VFF72_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93876.1"
                     /translation="MLEMRKVTACYGVLPILHGIDLDIQQGEIVALIGGNGAGKTTLM
                     MTIFGRPRVRDGHILFQGRDITAMPTHEVARLGIVQAPEGRRIFSRMTVLENLQMGAI
                     IANDPTHFAKDLDHVYTLFPLLKARQYQRGGTLSGGEQQMLAIARALMGRPTLLLLDE
                     PSLGLAPLIVKQIFAAIRALNTEYGLTVFLVEQSAYHALKLAHRGYVMVNGRITMSGS
                     GAELLGSPEVQAAYLEGGAQIAAQPGAQG"
     gene            1161..1505
                     /locus_tag="VFF72_11710"
     CDS             1161..1505
                     /locus_tag="VFF72_11710"
                     /inference="COORDINATES: protein motif:HMM:NF045145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93877.1"
                     /translation="MSWLSDDSLRVFLVLTVILGGGTAFLAGRGLARSWKPFGRIFFY
                     MVMLAAAVRFFHYSLFDGELLSLYYYLVTYIVLIGAACLGYRMMRTTQMVTQYRWLYE
                     RTSPLTWRARLG"
     gene            1579..>2190
                     /locus_tag="VFF72_11715"
     CDS             1579..>2190
                     /locus_tag="VFF72_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV93878.1"
                     /translation="MAGLLAAFALAALSAACGGGEESNTTPIAIVGPVTGQYASFGQQ
                     MKNGAELAVEDINAAGGVLGKKLDLDIGDDACDPKQAVAVANQMTAKGVALVAGHYCS
                     GSSIPASKVYAESDMVQISPASTNPAFTDERAGPNIYRVCGRDDQQGGVAGKYLASHF
                     SDRNIAIVHDKTAYGKGIADEVKKAINAAGKQETMYEAITAGEK"
BASE COUNT          386 a          706 c          711 g          387 t
ORIGIN      
        1 cttcgcgcgg cattggctcg accgggttgg gctgacgcgt cgcgccgacg agccggccgg
       61 gtcgctgccc tatggcgacc agagacgtct cgagatcgcg cgcgcgatgt gcacccggcc
      121 cctgctgctt tgcctcgacg aaccggcggc gggcctcaac gcgcgcgaga gcgacgggct
      181 gaatgagctg ttgttctcga tccgcgccga ggaaggcatc tcgctgctgc tcatcgagca
      241 cgacatgagc gtggtgatgc gcatctcgga ccacgtcgtg gtgctcgact acggcaagaa
      301 gatcgccgac ggcaccaccg acgaggcgcg caacgacccg cgggtcatcg ccgcctatct
      361 cggcgtgccc gacgaggagc ttccggtgat cgagcgggag gtaggcttat gagccagccg
      421 ctgctcgaaa tgcgcaaggt gacggcctgc tatggcgtgc tgccgattct gcacggcatc
      481 gatctcgaca tccagcaagg cgagatcgtc gcgctgatcg gcggcaacgg cgccggcaag
      541 acgacgctga tgatgacgat cttcggccgt ccgcgcgtaa gagacggcca catcctgttc
      601 cagggccgcg acatcaccgc catgcccacg catgaggtgg cgcggctcgg catcgtgcaa
      661 gcgcctgagg gccgccgcat cttctcgcgc atgacggtgc tggagaatct gcaaatgggc
      721 gccattatcg ccaacgaccc gacccatttc gccaaggatc tggaccacgt ctatacgttg
      781 tttcccctgc tcaaggcacg ccagtatcaa cgcggcggaa cactctccgg cggcgagcag
      841 cagatgctgg cgatcgcccg ggcgttgatg gggcgcccga cgctgctgtt gctcgacgag
      901 ccgtcgctcg gccttgcccc actgatcgtc aagcagatct tcgcggcgat ccgggcgctc
      961 aacaccgaat acggtctgac cgtcttcttg gtggagcaga gcgcctatca cgcgctcaag
     1021 ctcgcccatc gcggctatgt catggtcaat ggcaggatca ccatgagcgg ctcgggcgcc
     1081 gagctgctcg gcagccccga ggtgcaggcg gcttatctcg aaggcggcgc gcagatcgcc
     1141 gcccaaccgg gagcgcaggg atgagctggc tctccgacga cagccttcgc gtctttctgg
     1201 tgctgaccgt gatcctcggc ggggggaccg ctttcctggc tggacggggc ttggcgcgct
     1261 cgtggaagcc gtttggacgc atcttcttct acatggtgat gctggcggcg gcggtccgct
     1321 tcttccatta ttcgctgttc gacggcgaac ttctgtctct ctattactat ctcgtcactt
     1381 atattgtgct gatcggggcg gcgtgtctcg gctaccgcat gatgcggacg acacaaatgg
     1441 tgacgcaata tcgctggctt tacgaacgaa ccagcccgct cacctggcgc gcgcggctcg
     1501 gttgagccag tgcgcgctcg cgggcgaatt gctatagagt catcggagtt ttcgcagagg
     1561 gaggtaacaa tgaagcgtat ggccggactg ctcgcagcct tcgctctggc cgccttgagc
     1621 gccgcttgcg gcgggggcga ggagagcaac acgacgccga tcgcgatcgt cggcccggtc
     1681 acgggtcagt atgcctcctt cgggcagcag atgaagaacg gcgccgagct tgccgtcgag
     1741 gacatcaacg ccgccggcgg cgtgctcggc aagaagctcg atctcgatat cggcgacgac
     1801 gcctgcgatc cgaagcaggc cgtggcggtc gccaatcaga tgaccgccaa gggcgtcgcg
     1861 ctggtcgccg ggcattattg ctccggatcc tcgatcccgg cctccaaggt ctatgccgag
     1921 agcgatatgg tgcagatctc gccggcttcg accaatcccg ccttcaccga cgagcgcgcg
     1981 ggccccaaca tctatcgtgt ctgcggccgc gacgaccagc aaggcggcgt cgccggcaaa
     2041 tatctcgcca gccatttctc cgaccggaat atcgccatcg tgcacgacaa gaccgcctac
     2101 ggcaaaggca tcgccgacga ggtcaagaag gcgatcaacg cggccggcaa gcaggagacc
     2161 atgtatgagg ccatcaccgc gggcgagaag
//
LOCUS       DASRWS010000613         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4255418, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000613 DASRWS010000000
VERSION     DASRWS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4255418"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFF72_11720"
     CDS             complement(<1..355)
                     /locus_tag="VFF72_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZV93879.1"
                     /translation="MNTAVLHYDRRTIVLHWTTAALVVALWALGQTIDWFPKGTPRIF
                     ARSTHISLGVALAFVLVARIVWRLGARAVHLPPAGLGWLDRLATLTHWLLYVLLVATV
                     ALGITNAWVRGDTIFN"
     gene            650..2353
                     /locus_tag="VFF72_11725"
     CDS             650..2353
                     /locus_tag="VFF72_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93880.1"
                     /translation="MRKSNVPFFMSLAAAAVLAGCGGGGTPAFTTTSVSAAQGGSAMS
                     INGTLRLTIPAGALSQDTTVTIASVSDTPPSGTPIAANGAAFSVTFSGGATLSQAMTV
                     DMVSNGAPTHPQLGEIATESSGSWTRLSSNFFRTSDNHVIGLTKTPGVFIPVTRTLQA
                     MTGDSVARGQDTFLHETFGNEAFFGGVLGLHTLLNNLTPAQAVAAGVQVDISKVPAPI
                     AAVLTGADLAAKDAALQDPSVTRALLQAGAVVGVKAVYGNPGSDMATSAGITCALCHV
                     NVTPTTFQLTAGATSLPIGALAIDGKPNLAMNAGAILSLTPYVQTDPNLVAVLQSWGP
                     GKFDIRALPDNPLEDNTVNPTSTPPLWNFVDLEQQVYAYDWDGLFKSTATPNISLASQ
                     AEAVYDLVMHANGAFGTAGGTIAPQLSVTPPQSLLDALAAAETADPGNVIVASELYDV
                     EDFERSIVSPAPGSYDEALAEQGFVLFNGKAGCVGCHRTAEFTGPVVSTRITLTPPSG
                     GLAGGIKTPGLRGISSTAPYFHDGSAATLMDVVNTYSGRIVPTLSDSEKGALVEYLKS
                     L"
     gene            complement(2413..>2641)
                     /locus_tag="VFF72_11730"
     CDS             complement(2413..>2641)
                     /locus_tag="VFF72_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93881.1"
                     /translation="PAFWWMDAMVLLYLVFTVMLFVLEPYALRRHFDARAGRDPAAAL
                     RTMLRLHRVLLAASLVTIAGAVAGSHGWLIG"
BASE COUNT          452 a          930 c          883 g          376 t
ORIGIN      
        1 ggttgaagat cgtgtcgcca cggacccacg cgttcgtgat ccccagggcg acggtggcga
       61 cgagcagcac atagagcagc cagtgcgtga gcgtcgccag cctgtcgagc catcccagcc
      121 ctgcgggcgg gagatggacg gcccgggcgc cgaggcgcca gacgatccgt gcgaccagca
      181 cgaatgcgag cgcgacaccg agcgagatgt gcgtgctgcg cgcgaagatc cgcggcgtgc
      241 ccttgggaaa ccagtcgatg gtctgtccca gggcccacaa cgcaacgacc agtgcggcag
      301 tggtccagtg caggacgatc gtgcgacggt cgtagtgaag gacggctgtg ttcatctcgg
      361 tcattcatcg atggtaatgg tgtgccgatg ggcgacgcga gcgtgccaac gcgaagccgg
      421 gttcgcacat ctgacgcctc cctgaccggg aaagatggtc gacgcgagcg tgccaacgcg
      481 aagcccggct cgcacatctg acgcctccct gaccgggaaa cctcgtcacg ttcggctcgg
      541 ggtattccct gaacatcgcc ttccccgaat ccgccgggct cgtcctcgcg taccacgcgc
      601 atccgcaaaa acgcccggcg ccgccgggat cggatggagg agcctcgcaa tgcgcaagtc
      661 gaatgttccc ttcttcatgt ccctcgccgc ggcggcggtt ctcgccggct gcggtggcgg
      721 cggcacgccc gcgttcacga ccacgagcgt gagcgccgcc cagggcggca gcgccatgtc
      781 gatcaacggc acgctcaggc tgaccattcc tgccggcgcc ctcagccagg acacgacggt
      841 gaccatcgcc agcgtcagcg acacgccgcc gtcgggcacg ccgatcgccg ccaacggcgc
      901 ggccttcagc gtcacgttca gcggcggcgc gacgctcagc caggcgatga ccgtcgacat
      961 ggtttccaac ggcgctccga ctcatccgca gctcggtgaa atcgccaccg agtcgagcgg
     1021 cagctggacc cgactgtcgt cgaatttctt tcgcacgagc gacaaccatg tgatcggcct
     1081 gacgaagacg ccgggcgtct tcatcccggt gacccggacg ctccaggcca tgaccggcga
     1141 cagcgtcgcg cgcgggcagg acacctttct ccacgagacc ttcggcaacg aggcgttctt
     1201 cggcggcgtg ctgggcctgc ataccctgct caacaacctc acgcccgcgc aggccgtcgc
     1261 tgccggcgtg caggtcgaca tctcgaaggt gccggcgccg atcgcggcgg tgctgaccgg
     1321 cgccgatctc gccgccaagg acgcggccct gcaggatccg tcggtgacgc gcgcgctcct
     1381 gcaagcgggc gcggtggtcg gcgtgaaggc ggtctacggc aatccgggaa gcgacatggc
     1441 cacttcggcc ggcatcacct gcgcgctctg ccacgtcaac gtcacgccga cgacgttcca
     1501 gctgacggcg ggcgcgacat cgctgccgat cggcgcgctc gccatcgacg gcaagccgaa
     1561 cctcgccatg aacgccggtg ccatcctgtc gctcacgcct tacgtgcaga ccgatcccaa
     1621 cctggtggcg gtgctgcaga gctggggccc gggcaaattc gacatccgcg cgcttcccga
     1681 caacccgctc gaggacaaca cggtcaaccc gacgtcgacg ccgccgctgt ggaacttcgt
     1741 cgatctcgag cagcaggtct acgcctacga ctgggacggc ctgttcaaga gcacggccac
     1801 gccgaacatc tcgctggcca gccaggccga ggcggtctac gacctggtga tgcacgccaa
     1861 cggcgcgttc ggcaccgccg gcggcaccat tgcgccgcag ctgtcggtga cgccgccgca
     1921 gtcgctgctc gatgcactgg ccgcggcgga gacggcggac ccgggcaacg tcatcgtcgc
     1981 gagcgagctc tacgacgtcg aagacttcga gcgcagcatc gtcagccccg ccccgggcag
     2041 ctatgacgag gcgctcgccg agcagggctt cgtcctcttc aacggcaagg ccggctgtgt
     2101 cggctgccac aggacggccg agttcacggg cccggtcgtg tcgacgcgga tcacgctgac
     2161 gccgccttcg ggcggattgg ccggcggcat caaaaccccg gggctgcgtg gcatctcgtc
     2221 gacagcgccg tacttccacg acggcagcgc cgcgacgctg atggacgtcg tcaacacgta
     2281 ttcggggcgg atcgttccga cgctcagcga cagcgagaag ggcgcgctgg tcgaatacct
     2341 caagtcgctc taggcggcat cggcgcgggg cctgctcacg cggcgcgaaa tcagccgtcg
     2401 cgcgaacggg ccctatccga tcagccagcc gtggctgccg gcgacggcgc cggcgatcgt
     2461 gacgaggctc gccgcgagca gcacgcgatg caggcgcagc atcgttcgca gcgccgccgc
     2521 cggatcacgc ccggcgcggg catcgaagtg ccggcgcagg gcgtagggct ccagcacgaa
     2581 cagcatgacg gtgaagacga ggtagaggag caccatcgcg tccatccacc agaacgcggg
     2641 c
//
LOCUS       DASRWS010000614         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2835552, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000614 DASRWS010000000
VERSION     DASRWS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835552"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFF72_11735"
     CDS             complement(<1..986)
                     /locus_tag="VFF72_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZV93882.1"
                     /translation="MTSTHRPADARPAADGRQTQFLTVISRDEATERFRRHLELAPLG
                     FETVALGAALGRVLADDVIAEVDVPGFDRSNVDGFALQAADTFGATEESPRHVERSGE
                     LLTPGVVPAVAIGRGQATVIATGAMLPRGADAVVMVEHTEIAPAGGASSVEISRAVAP
                     GDNVAFAGTDIARGETVLRGGQQLTSREIGVLAAIGVAEVRVCRRPRVAILSTGDEIV
                     PPGSPLPLGAIYDSNAAIVGAAVAELGGEPIQLGVVPDDAHKLADALDIALQYDVVLL
                     SGGTSKGAGDLSYRVASRLGDPGIVAHGVALKPGKPICMAVSQGKPVVILPG"
     gene            complement(1016..2230)
                     /gene="glp"
                     /locus_tag="VFF72_11740"
     CDS             complement(1016..2230)
                     /gene="glp"
                     /locus_tag="VFF72_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZV93883.1"
                     /translation="MRGFARRAEVGVAWQWIDDHAARLDAQIVEAEHATGRVLANDVA
                     ATLDVPAFDRSAMDGFALRGDETTGASEYNPLTFAVIGQALPARPFEGTVLAGQAVRI
                     MTGAPMPRGADAVAPAEYARESPQGAEISSAFSVGKNVGRLGEDIARGATVLEAGRRL
                     RPQDLGVLASLGLPRIEVIREPRVRIVVTGNEVVAPGRPKSAYEIYDANSAMLRGLVG
                     RDGGLAEGPVRTGDDPGRIREAITAGGADVVLVSGGTSVGTEDHAARLLAQSGELAIH
                     GIAMRPSSPAGFGRIGSTLVFLLPGNPVSCLCAYDFFAGRAIRLRGGRTSQWPYALLR
                     ASVGRKIVSAIGRVDYVRVRIRDGLVEPLALSGASVLSSTVRADGFVVVPAESEGLAP
                     QSAVDVYLYDPA"
     gene            complement(2381..2890)
                     /locus_tag="VFF72_11745"
     CDS             complement(2381..2890)
                     /locus_tag="VFF72_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93884.1"
                     /translation="MTRPPLRSLLPLVVCVTVATTTPAVAAGSCDEAYQAGIKSLQTP
                     HHVYSTTTMPGGKTRASEAIYAGGVEYLKIGNKWQRSRMKPQDMIELAQEKMKTHPDT
                     CTRIGDQTAGGQAVAVYKVHSNEMGTDQVVSVFKSSGLIQGTTVSLPNQSMVIRYDYA
                     NVQAPAGVK"
     gene            complement(2929..>3234)
                     /locus_tag="VFF72_11750"
     CDS             complement(2929..>3234)
                     /locus_tag="VFF72_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93885.1"
                     /translation="SARRTLDVPRAASHFILLAAGLAGVLAAPPAKAACPPGYRSKGA
                     QCIPGPVEPHRPYTAHSNVPSSAAAVHPAHKPGVITEEIDRRKVKPQPGAPIERAGR"
BASE COUNT          460 a         1099 c         1202 g          473 t
ORIGIN      
        1 aagccgggaa ggatgacgac gggcttgcct tggctcaccg ccatgcagat cggcttgccg
       61 ggcttgagcg cgacgccgtg ggcgacgatg cccggatcgc cgaggcggct ggccacccgg
      121 tacgagaggt cgccggcgcc cttcgaggtg ccgcccgaca ggaggacgac gtcgtactgc
      181 agcgcgatat ccagtgcgtc ggcgagcttg tgcgcatcgt cgggcacgac gccgagctgg
      241 atcggttcgc ccccgagctc ggcgaccgcg gcgccgacga tggccgcgtt cgagtcgtag
      301 atggcgccca gcggcagcgg cgagccgggc ggaacgatct cgtcgcccgt cgacaggatg
      361 gccacgcgcg gacgacggca gacgcggact tcggccacgc cgatcgccgc gagcacgccg
      421 atctcgcgcg aggtgagctg ctgcccgccg cgcagcacgg tctcgccgcg ggcgatgtcg
      481 gtcccggcga aggcgacgtt gtcgcccggc gcgacggcac ggctgatctc gaccgacgac
      541 gcgccgccgg ccggcgcgat ttcggtgtgc tcgaccatga cgaccgcgtc ggcgccgcgc
      601 ggcagcatcg cgccggtggc gatcaccgtg gcctggccgc ggccgatcgc cacggcgggg
      661 acgacgcccg gggtcagcaa ttcgccgctg cgctcgacgt gccgcggcga ctcctcggtg
      721 gcgccgaagg tatcggcggc ctggagggcg aagccgtcga cgttcgaacg atcgaagccg
      781 ggcacgtcga cttcggcgat cacgtcgtcg gcgagcaccc ggccgagcgc ggcgccgagc
      841 gcgactgtct cgaagccgag cggcgccagc tcgaggtggc ggcgaaagcg ttccgtggcc
      901 tcgtcgcggc tgatgacggt gaggaactgc gtctggcgtc cgtcggctgc cggccgcgcg
      961 tcggcagggc ggtgtgtgct cgtcatggaa tcggcgtcgt cgtatgaaat ggacctcagg
     1021 ccgggtcgta gaggtagacg tcgaccgcgc tttgcggagc gaggccctcg ctttcggccg
     1081 gcacgacgac gaagccgtcg gcgcgcacgg tcgacgacag caccgacgcg ccgctcagtg
     1141 cgagcggctc gacgagaccg tcgcgaatgc ggacgcgaac gtagtcgacc cggccgatgg
     1201 cggagacgat cttgcgcccg accgaggccc tgagcaacgc gtatggccat tgcgaagtcc
     1261 ggccgccgcg caggcgtatc gcccgcccgg cgaagaagtc ataggcgcaa agacacgaca
     1321 ccggattgcc gggcagcagg aacaccagcg tcgagccgat gcggccgaag cccgccgggc
     1381 tcgagggccg catcgcgatg ccgtggatgg cgagctcgcc cgactgcgcc agcaggcgcg
     1441 cggcgtggtc ttccgtgccg acgctcgtgc cgcccgagac gaggacgacg tcggcgccgc
     1501 cggcggtgat cgcttcgcgg atgcgcccgg gatcgtcgcc ggtgcggacc gggccctcgg
     1561 caaggccgcc atcgcggccg acgaggccgc gcagcatggc cgagttggcg tcgtagatct
     1621 cgtaggctga tttcggacgg ccgggtgcca ccacctcgtt gccggtgacg acgatgcgca
     1681 cgcggggctc gcggatcacc tcgatgcggg gcaggcccag cgaggcgagc acgccgaggt
     1741 cctgcgggcg caggcgccgg ccggcctcga gcacggtggc gccgcgggcg atgtcctcgc
     1801 ccaggcgccc gacgttcttg ccgaccgaaa acgccgacga gatctcggcg ccctgcggcg
     1861 attcgcgggc gtactcggca ggcgccacgg cgtcggcgcc gcggggcatc ggcgcgccgg
     1921 tcatgatgcg caccgcctgg ccggcgagga ccgttccttc gaacggccgg gccggcaacg
     1981 cctggccgat cacggcaaag gtcaacgggt tgtattcgct cgcgcccgtg gtctcgtcgc
     2041 cgcggagcgc gaagccgtcc atcgcggagc gatcgaatgc cggcacgtcg agggtcgcgg
     2101 cgacgtcgtt cgccagcacg cggcccgtcg cgtgctcggc ctcgacgatc tgcgcgtcca
     2161 ggcgcgccgc gtggtcatcg atccattgcc aggcgacgcc gacctcggcc cgtcgggcga
     2221 agccccgcat gcgcacctcg cccggatcgg gcccgtttgc agacatcgcg ccggccacgc
     2281 tttctcgtca agtcgaaatt cactctatca cctgacggaa cagacgcgtc acgacgaccc
     2341 cgcgacggcg gcgcggatcc gcgccgggag cgaggccctt ctacttgacg ccggccgggg
     2401 cctgcacgtt ggcgtagtcg tagcggatca ccatggactg gttcggcagc gaaacggtcg
     2461 tgccctggat caatccgctc gacttgaaga cgctcaccac ctggtcggtg cccatctcgt
     2521 tgctgtgcac cttgtacacc gccacggcct ggccgccggc ggtctggtcg ccgatgcggg
     2581 tgcaggtgtc ggggtgcgtc ttcatctttt cctgcgcgag ctcgatcatg tcttgcggct
     2641 tcatgcggct gcgctgccac ttgttgccga tcttcaggta ttcgacgccg ccggcgtaga
     2701 tggcttcgct ggcgcgcgtc tttcccccgg gcatcgtcgt cgtcgagtag acgtggtgcg
     2761 gcgtctgcaa cgacttgatg ccggcctgat aggcctcgtc gcagctgccg gctgcgacgg
     2821 ccggggtggt cgtcgcgacc gtcacgcaga cgacaagcgg cagcagggac cggaggggtg
     2881 gacgcgtcat gaaggatctc ctcggtgaaa gttcgacgaa gtcgtggatc agcgcccggc
     2941 ccgttcgatc ggcgctccgg gctgcggctt gaccttgcgc cgatcgattt cctcggtgat
     3001 cacgcccggc ttgtgcgcgg ggtgaaccgc cgcggcggaa gacgggacgt tgctgtgcgc
     3061 ggtgtaagga cgatgcggct cgacgggccc gggaatgcat tgcgctccct tggatcgata
     3121 gccgggcgga cacgcggcct ttgcgggtgg cgccgccagg acgccagcca ggccggcggc
     3181 gagcaggatg aagtgcgaag cggctcgcgg aacgtcgagg gtgcgtcggg cgga
//
LOCUS       DASRWS010000615         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2525132, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000615 DASRWS010000000
VERSION     DASRWS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525132"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFF72_11755"
     CDS             complement(<1..318)
                     /locus_tag="VFF72_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV93886.1"
                     /translation="MASTMTADAQDAYVPDQRSRRLVTLASLIGTTVEWYDFFLYGTI
                     TGLVFNKLFFPAGDPFVSTMLAYTVYAVGFVTRPVGGVIFGHFGDRIGRKPLLVLTLL
                     IMGI"
     gene            complement(507..1943)
                     /gene="phnY"
                     /locus_tag="VFF72_11760"
     CDS             complement(507..1943)
                     /gene="phnY"
                     /locus_tag="VFF72_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HZV93887.1"
                     /translation="MNARELRREAMRIGGESVHRDRVIEVFNPYTEQLIGSVPKATLD
                     DVRHAFSVARAYRSRLTRYERSAILNKAAAIVRSRLEELSDLITAESGLCKKDSLYEI
                     GRVSDVLLYGANECLKDDGQIFSCDLTPHGKKRRVYTQRDPLLGVISAITPFNHPMNQ
                     VAHKVVPAIVTNNRMVLKPSEKVPFSAIVLADILYEAGLPPPMYQVVTGDPREIADEL
                     ITNADVDLITFTGGVAIGKYIASKAGYRRMVLELGGNDPLIVMEDADLDEASTLAAAG
                     SYKNSGQRCTAVKRMLVQESVADRFVELLVEKTRAWSYGDPMDGKMEMGTVIDEAAAR
                     YCEDRVNEAVAAGAKLLVGNQRRGALYSPTVVDHVTREMPLVLQETFGPVSPVLRFET
                     IDDAIRLSNSTAFGLSSGVCTNRLDYISRFVSELEVGSVNVREVPGYRLELTPFGGIK
                     DSGLGYKEGVQEAVKSFTNIKTYSLPWA"
     gene            complement(1940..>2288)
                     /locus_tag="VFF72_11765"
     CDS             complement(1940..>2288)
                     /locus_tag="VFF72_11765"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZV93888.1"
                     /translation="ALAHSISYDMTMHHGLPHGIACSFTLGMVLERALGADAERDAVL
                     ARVFDVPLGQAPAVLDRFLESLGVSTRFETYGVTAAQSERMVVSALDGVRGRNFIGAA
                     ASTSATVNQGATA"
BASE COUNT          369 a          732 c          779 g          408 t
ORIGIN      
        1 gatgcccatg atcagcagcg tcagcacgag caatggcttt cgtccgatcc tgtcgccgaa
       61 gtgcccgaag atgacaccgc cgaccggccg ggtgacgaag cccacggcgt acaccgtgta
      121 ggcgagcatc gtcgagacga acggatcgcc ggccggaaag aacagcttgt tgaagaccag
      181 gccggtgatc gtgccgtaga gaaagaagtc gtaccactcg accgtggtgc cgatcaggct
      241 tgccagcgtg accaggcgcc tgctgcgctg gtcgggcaca taggcgtcct gtgcatccgc
      301 cgtcatcgtt gaggccatcg tctgtctccc ttgtctctgg gtacctgggt accctgtttt
      361 tcaattcccg tcttcgcgag cgggggcgag tctcgacttg gataaggaaa gagaaaagta
      421 tcgaagggcg ccgggcggcg gggcaatcca ggttttctcg atgcgttcgc tcaagggcgc
      481 catgaggcac gcgttgccgc ttcgcgtcac gcccacggaa gcgagtaggt cttgatgttg
      541 gtgaagctct tcaccgcttc ctgcacgcct tccttgtagc cgaggcccga gtccttgatg
      601 ccgccgaacg gcgtgagctc gaggcggtag cccggcacct cgcggacgtt caccgagccg
      661 acctcgagct cggagacgaa gcgggagatg tagtcgagcc ggttcgtgca gacgcccgac
      721 gagaggccga aggcggtgga gttggaaagc ctgatcgcgt cgtcgatcgt ctcgaagcgc
      781 agcaccggcg agaccgggcc gaaggtttcc tgcagcacca gcggcatctc gcgcgtgacg
      841 tgatcgacga ccgtcggcga gtagagggcg ccgcggcgct ggttgccgac gagcagcttc
      901 gcgccggcgg ccaccgcttc gttgacccgg tcctcgcagt agcgggccgc ggcctcgtcg
      961 atgacggtgc ccatctccat cttgccgtcc atcgggtcgc cgtagctcca ggcccgggtc
     1021 ttctcgacca gcagctcgac gaagcggtcg gcgaccgatt cctgcaccag catgcgcttg
     1081 accgcggtgc aacgctggcc cgagttcttg taggagcccg cggcggcgag ggtcgaggct
     1141 tcgtcgaggt cggcgtcttc catgacgatc agcgggtcgt tgccgccgag ctcgagcacc
     1201 atccggcgat agcccgcctt cgaggcgatg tacttgccga tcgcgacacc gcccgtgaac
     1261 gtgatcaggt cgacatcggc gttggtgatc agctcgtcgg cgatctccct cgggtcgccg
     1321 gtgaccacct ggtacatggg cggcggcagg cccgcttcgt acaggatgtc ggcgagcacg
     1381 atcgccgaga acggcacttt ctcggaaggc ttgagcacca tccggttgtt ggtgacgatc
     1441 gccggcacga ccttgtgggc gacctggttc atcgggtggt tgaagggcgt gatggcgctg
     1501 atgacgccga gcagcggatc gcgctgcgta tacacgcgcc gcttcttgcc gtgcggcgtc
     1561 aggtcgcacg agaagatctg gccgtcgtcc ttcaggcact cgttggcgcc gtagagcagc
     1621 acgtcgctga cgcggccgat ctcgtagagc gaatccttct tgcacaggcc gctttcggcg
     1681 gtgatgagat ccgacagctc ctcgaggcgg ctgcgcacga tcgcggccgc cttgttgagg
     1741 atggcggatc gctcgtagcg cgtgagcctg gaccggtacg ccctggccac cgagaaggcg
     1801 tgccgcacat cgtcgagcgt cgccttgggc acgctgccga tcagttgctc ggtgtaaggg
     1861 ttgaagacct cgatgacccg gtcgcggtgc accgattcgc caccgatccg catcgcttcc
     1921 cgccgcagtt cccgcgcatt catgctgtcg ctccctgatt gaccgtggcg ctcgtgctcg
     1981 cggcggcgcc gatgaaattc ctgccgcgca cgccgtcgag ggcgctgacg accatccgct
     2041 ccgactgcgc tgccgtcacg ccataggtct cgaagcgcgt gctgacgccg agcgattcga
     2101 ggaaccggtc gagaacggcc ggcgcctggc cgagcggcac gtcgaacacg cgtgccagca
     2161 ccgcgtcgcg ctcggcgtcg gcgccgagcg cgcgctcgag caccatgccg agcgtgaacg
     2221 agcaggcgat gccgtgcggc agcccgtggt gcatcgtcat gtcgtaggag atcgagtggg
     2281 cgagcgcc
//
LOCUS       DASRWS010000616         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1955697, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000616 DASRWS010000000
VERSION     DASRWS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955697"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="VFF72_11770"
     CDS             <1..1685
                     /locus_tag="VFF72_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262850.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HZV93889.1"
                     /translation="HLDGHALLSNAGHLKGSAQWIRFPRVVCKTWVYDNGRAPAVLMG
                     DAAHTAHFSIGSGTKLALEDSIELAHSIARHPGDLAAALQHYEDVRSIEVLKIQNAAR
                     NSTEWFEHVDRYVNLPPEQFAYSLLTRSQRISHENLRLRDKSYVETYEDWIAGQAGLP
                     ARIDVAGGGWKHSVPPMLTPFTLRGVTLKNRIVVSPMAQYSAVDGVAGDYHLVHLGAR
                     AMGGAAMVVVEMTCPSADARITPGCPGLWNTVQRDAFRRIVDFVHANSDAKIAMQLGH
                     AGAKGSTRVPWEGEDQPLESGNWPLISASPQQYIDGISDWSRAMTRDDMDRVRDDFVL
                     STRLAAEAGFDWLELHCAHGYLLSSFISPLTNQRTDEYGGSLANRLRYPLEVFHAMRE
                     VWPKQLPISIRISAHDWVEGGITPDDAVEIARAFKAAGADLIDCSSGQVSKKQQPVYG
                     RMYQTPFADRVRNEAGIPTMAVGAISEADHVNSIIAAGRADLCAVARPHLANPAWTLL
                     EAAKIGVTEVAWPKQYLSGKQQLERNLERERSMAAQGAGLSALEQANRALGV"
     gene            1782..2312
                     /locus_tag="VFF72_11775"
     CDS             1782..2312
                     /locus_tag="VFF72_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZV93890.1"
                     /translation="MKTTDPSHALLEDAQELGHEARAGHDDHAALRLWLRMLASTTHI
                     ETEIRRRLRERFDISLARFDYLAQLYRYRDGMKMRELSRYLMVTGGNVTGLTDDLAHE
                     GLVVRESSPQDRRAWIVRLTAKGRRQFEAMAREHEGWILDLFSGLDAATVQQLYAQLG
                     QLRVHLVRGEQGDANA"
BASE COUNT          448 a         1020 c          998 g          421 t
ORIGIN      
        1 agcatctcga cggccacgcg ctgctgtcga acgccggcca cctgaagggc tcggcgcagt
       61 ggatccgctt tccgcgcgtc gtctgcaaga cctgggtcta cgacaacggc cgcgcgccgg
      121 cggtgctgat gggagacgcc gcgcacacgg cgcacttctc gatcggctcg ggcaccaagc
      181 tcgccctcga ggactcgatc gagctggcgc actccatcgc ccgccatccg ggcgacctcg
      241 ccgccgccct tcagcactac gaggacgtgc gcagcatcga ggtgctgaag atccagaacg
      301 cggcgcgcaa ctcgaccgaa tggttcgagc acgtcgaccg ctacgtcaac ctgccgcccg
      361 agcagttcgc ctattcgctt ctgacgcgca gccagcgcat cagccacgag aacctgcgcc
      421 tgcgcgacaa gagctacgtc gagacctacg aagactggat cgccggccag gccggcctgc
      481 cggcgcgcat cgatgtcgcg ggcggcggct ggaagcattc ggtgccgccg atgctcacgc
      541 ccttcacgct gcgcggcgtg acgctgaaga accgcatcgt cgtctcgccg atggcgcagt
      601 actcggcggt cgacggcgtc gccggcgact atcacctcgt ccatctcggc gcccgcgcca
      661 tgggcggcgc ggcgatggtc gtggtcgaga tgacctgtcc cagcgccgat gcccgcatca
      721 ccccgggctg cccggggctg tggaacacgg tgcagcgcga tgccttcagg cggatcgtcg
      781 atttcgtcca cgccaacagc gacgcgaaga tcgcgatgca gctcggccac gccggcgcca
      841 agggctcgac ccgcgtgccg tgggaaggcg aagaccagcc gctcgagagc ggaaactggc
      901 cgctgatctc ggcctcgccg cagcagtaca tcgacggcat cagcgactgg tcgcgggcga
      961 tgacgcgcga cgacatggat cgcgtccgcg acgacttcgt cctctcgacg cggctggccg
     1021 ccgaggccgg cttcgactgg ctcgagctgc actgcgcgca cggctacctg ctgtcgtcct
     1081 tcatctcgcc gctcaccaac cagcgcaccg acgagtacgg cggctcgctc gccaaccgcc
     1141 tgcgctatcc gctcgaggtc ttccacgcga tgcgcgaggt ctggccgaag cagttgccga
     1201 tctcgatccg catctcggcg cacgactggg tcgaaggcgg catcacgccc gacgacgcgg
     1261 tcgagatcgc gcgtgccttc aaggccgccg gcgccgacct gatcgactgt tcgtcgggcc
     1321 aggtgagcaa gaagcagcag ccggtttacg gccgcatgta ccagacgccg ttcgccgatc
     1381 gcgttcgcaa cgaggccggc atcccgacga tggcggtggg cgcgatcagc gaggccgacc
     1441 acgtcaacag catcatcgcc gccggtcgcg ccgatctctg cgccgtcgcc cgtccgcacc
     1501 tcgccaaccc ggcgtggacg ctgctcgagg cggccaagat cggcgtcacc gaggtggcgt
     1561 ggccgaagca gtacctttcg ggcaagcagc agctcgagcg caatctcgag cgcgagcggt
     1621 cgatggcggc gcagggcgcg ggcctgagcg ccctcgagca ggcgaaccgc gccctcggcg
     1681 tgtgaggcga tgaagctgcc gcgctcgcga acacgttcac ggcccttcga cggggccgag
     1741 tcattccgct ccatgcctgg cctcgctttc cgatgcatgc catgaagacg accgacccgt
     1801 cgcacgcgct gctcgaagac gcgcaggagc tcggccacga ggcgcgggcc ggccacgacg
     1861 accacgccgc gctcaggctc tggctgcgca tgctggcgag caccacgcac atcgagacgg
     1921 agatccggcg tcgcctgcgc gagcgcttcg acatctcgct ggcccgcttc gactacctgg
     1981 cccagctcta tcgctatcgc gacggcatga agatgcgcga gctgtcgcgc tatctcatgg
     2041 tcaccggggg caacgtcacc ggcctcaccg acgacctggc gcacgaaggc ctcgtcgttc
     2101 gcgagagcag cccgcaggac cggcgcgcct ggatcgtccg gctcacggcc aagggccggc
     2161 gccagttcga agcgatggcg cgcgagcacg aaggctggat cctcgatctc ttctcggggc
     2221 tcgacgcggc cacggttcag cagctgtacg cgcagctcgg gcagctgcgg gtgcatctgg
     2281 tgcggggcga gcagggcgat gccaatgcct gacgttcatg ccgggccggc gatgggcggg
     2341 caatgcggcg ttcgggccag ccggtgcgct tcgctggagc ggtcgggcat gagccggcaa
     2401 aggagtgtcc gggcaggccg gggcgcttcg ctggagcggt cggcgctgcg cgccgactgc
     2461 cctgcggtgc tcggtcttgc ggcccgccgc atgactcgct tcgttcgctt tgctcactgc
     2521 gctcggacag atgcggcgag tcagtggtgg atgcgcgctg cgcgcgcggc cacaagccct
     2581 gtgctcctcg gcgccccaga ggcgcgcccc gacctgcccg gacgctcctt tgcagcagcg
     2641 ctggtggttc tcgcgtgaaa gaggcgacgc tggtgcagcg cggcgggcgg tgcccggcgg
     2701 gggcgatttc cgtggcgacg agcagcgcag cttcggggtc ggcacgcgcg cagcgcgtgc
     2761 atcgtgaact gactcgccgc gtctgttcga gcgcagtgag cgaagcgaac gaagcgagtt
     2821 acgcggcgcg accccgaagc gagcagcgca gtgaagtcgg cgcgcagcgc cgaccgccac
     2881 ggcatga
//
LOCUS       DASRWS010000617         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1115649, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000617 DASRWS010000000
VERSION     DASRWS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115649"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VFF72_11780"
     CDS             complement(<1..1456)
                     /locus_tag="VFF72_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93891.1"
                     /translation="MPASRFLSASGRASVFVVAMLVFSGTATAAAAASSGDDPPTHSK
                     LDAPLFYQLLLGEIELRDGDAATAYKVLLDAAERAKDEALFRHVTDIALRARAGEQAL
                     GAVRAWRKAIPGSQQAIRYQLQLLVALNRVGEIQEPLTALLDLTPKPSLPKMIDAVPR
                     FLARSTDHAAVADVIERTMKPFAANPETRTSALVATGRGWLAAGDSAKALDFAQRASA
                     ADPQADGPALLALDLLPAVPAAEAIVTTRLARTPANPAVRMLYVRTLASEQRIADAVG
                     QTTTLVESDPGLAPPWLTLGALQLDARHPREAITALETYVRLVQGGASVTFAPAPAAS
                     APPTGNEAASDDGDDDTPSNQSNALTQAWLLLSQAAEQQGDLPGAERWLAHIDNSQRA
                     LDVQSRRASLLAKQGKMQEARELIRRVPEHSDTDARAKLLAEANLLREAKQWSDAETV
                     LAQANKLFPDDVDLLYEEAMIDEKLDRLDDMERLL"
     gene            1610..2422
                     /gene="mutM"
                     /locus_tag="VFF72_11785"
     CDS             1610..2422
                     /gene="mutM"
                     /locus_tag="VFF72_11785"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZV93892.1"
                     /translation="MPELPEVEVTRLSFADRIDGARVTAVRVGKALRWPLGCVPARLV
                     DQRVGAPVRRGKYLWLPLERGGLLMHLGMSGSLAFGPAASPGPHDHFDLVTANGTLRL
                     TDPRRFGAVVWSPSIDSGTAARLLARLGVEPFDPAFDGAHLHAAMRRRKVSVKQALLG
                     GDIVVGAGNIYACEALFEARIDPRTRCDRISRPRAERLAAAIRSVLARAVELGGSTLR
                     DFRDTHGAAGAFQLQARVYGRAGLPCVRGDGTVRRIVQGGRATFFCPGCQKR"
     gene            2577..>3062
                     /locus_tag="VFF72_11790"
     CDS             2577..>3062
                     /locus_tag="VFF72_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZV93893.1"
                     /translation="MTPSFATSLDALGGWRAALGERLESLARFLAEHNLAQGPAGEQL
                     AALRDRLGNEKLVVAFVAEFSRGKSELINAIFFADTGRRILPATPGRTTMCPVELGWR
                     GDEPASLMLLPIETRLEGLALGELRTQPRAWRRLDLDVHDADQLAHSLLEVTRTEFVS
                     EE"
BASE COUNT          406 a         1061 c         1110 g          485 t
ORIGIN      
        1 gcagcagccg ctccatgtcg tcgaggcgat cgagcttctc gtcgatcatg gcctcttcgt
       61 agaggaggtc gacgtcgtcg gggaagagct tgtttgcttg cgccagcacc gtctcggcat
      121 cgctccactg ctttgcttcg cgcagcaggt tggcctcggc gagcagcttg gcgcgggcgt
      181 cggtgtcgga gtgctcggga acgcggcgaa tcagctctcg cgcctcctgc atcttgcctt
      241 gcttggcgag gagcgaagcg cgacgcgact ggacgtcgag ggcgcgctgc gaattgtcga
      301 tgtgggccag ccagcgctcc gcgccaggca ggtcgccttg ctgctcggcg gcctgcgaca
      361 acagcagcca ggcctgcgtc agcgcgttcg actggttcga gggggtgtcg tcgtcgccgt
      421 cgtcgctggc cgcctcgttg ccggttggcg gcgccgacgc cgcgggggcg ggcgcgaagg
      481 tcaccgaggc accgccctgg acgagccgga cgtacgtttc gagcgccgtg atcgcttccc
      541 gcggatggcg ggcatcgagc tgcagggcac ccagcgtgag ccacggcggc gccaggccgg
      601 ggtcgctttc gaccagcgtc gtcgtctgcc cgaccgcgtc ggcgatgcgc tgttcggaag
      661 cgagcgtgcg gacgtacagc atgcggaccg cagggttcgc cggcgtcctc gccaggcgcg
      721 tcgtgacgat cgcctcggcg gccggaacgg cgggcaacag gtcgagggcg agaagggccg
      781 gtccgtcggc ctgcgggtcg gccgccgatg cgcgctgcgc gaagtcgagc gccttggcgc
      841 tgtcgccggc ggccagccag ccgcgtcccg tggcgacgag tgccgaggtt cgcgtctcgg
      901 gattggccgc gaacggcttc atcgttcgct cgatgacgtc cgcgacggcg gcatggtcgg
      961 tgctcctggc gagaaagcgc ggcaccgcgt cgatcatctt cggcagcgac ggcttgggcg
     1021 tcaggtcgag gagcgcggtc aacggctcct ggatctcgcc gacgcgattg agcgcgacca
     1081 gcaactgcaa ctggtagcga atcgcctgct gcgaaccggg gatggccttg cgccaggcgc
     1141 gcaccgcgcc gagcgcctgt tcgccggcgc gcgctcgcag agcgatgtcg gtgacgtggc
     1201 gaaagagggc ttcgtccttg gcacgctcgg ccgcgtcgag cagcaccttg taggcggtcg
     1261 cggcgtcgcc atcgcgcaac tcgatctcgc cgagcagcaa ctggtagaag agcggcgcgt
     1321 cgagcttcga gtgggtcggc ggatcgtcgc cgctgctggc cgctgccgcc gccgtcgcgg
     1381 tgcccgaaaa cacgagcatc gcgacgacga agaccgaggc gcggcccgaa gcggaaagaa
     1441 agcgggacgc cggcatcgat gactcctctg gtcggaaacg attctagtgt ggaccccggc
     1501 gacgaagggc gaacgaaggc gcgtcgaaga cctcggatcc gttccgatcg cgctgcgtcg
     1561 tgataattcc ccgccggcac gcaaagagcc caccgacacc gcgcccctca tgcccgagct
     1621 tcccgaagtc gaagtcaccc gtctcagctt cgccgaccgc atcgacggcg cgcgcgtcac
     1681 ggcggtgcgg gtcggcaagg ccttgcggtg gccgctcggc tgcgtgccgg cgcgtctcgt
     1741 cgaccaacgg gtcggcgcgc cggtccgtcg cggcaaatac ctgtggctgc cgctcgagcg
     1801 cggcggcctc ctgatgcacc tgggcatgtc gggatcgctg gcgttcggtc cggccgcttc
     1861 gccggggccg cacgaccatt tcgatctcgt caccgccaac ggcaccttgc gcctcaccga
     1921 tccgcgccgt ttcggtgcgg tggtgtggtc gccttcgatc gacagcggaa cggcggcgcg
     1981 gctgctcgcc cggctcggcg tcgagccgtt cgatccggcc ttcgacggtg cccatctgca
     2041 tgccgcgatg cgccgacgca aggtctcggt gaagcaggcg ctgctcggcg gcgacatcgt
     2101 cgtcggcgcc ggcaacatct acgcgtgcga agcgctgttc gaggcgcgca tcgatccgcg
     2161 cacgcgctgc gaccgcatca gccggcctcg agccgagcgc cttgcggcag cgatccggtc
     2221 cgtgcttgcc cgcgccgtcg agctcggtgg ctcgaccttg cgcgatttcc gcgacacgca
     2281 tggcgctgcc ggcgccttcc agctgcaggc ccgcgtctat gggcgcgccg gcctgccctg
     2341 cgtgcgtggc gacggcaccg tcaggcgaat cgtccagggg ggccgcgcca ccttcttctg
     2401 tcccggctgc cagaagcgtt gatgcggcgc gacgcggcgt gcgaaaggtc cgtcgcccgg
     2461 tgtgagcagt tgtttcgccg gcatggaatc gacgagcgct gcgctaggat gcctcgtgat
     2521 cgtgcctgcc caagggccgc cgccggcgtg ccgtgcggcg gcgcggcgcg atgccgatga
     2581 caccttcgtt tgccaccagt ctcgacgccc tcggcggatg gcgcgccgcg ctcggcgagc
     2641 ggctggaatc gctggcccgc ttcctggccg agcacaacct cgcgcagggc cccgccggcg
     2701 agcagctcgc ggcgctgcgc gatcggctcg gcaacgagaa gctcgtcgtc gcgttcgtcg
     2761 ccgagttctc gcgcggcaag tcggagctca tcaacgcgat cttcttcgcc gacaccggtc
     2821 gccgcatcct gcctgccacg ccgggccgca cgacgatgtg cccggtcgag ctcggctggc
     2881 gaggcgacga gcccgcctcg ctgatgctgc tgccgatcga gacgcgtctg gaaggcctgg
     2941 cgctgggcga gctgcggacg cagccgcggg cctggcgccg gctcgatctc gacgtgcacg
     3001 acgccgacca gctcgcccat tcgctgctcg aggtcacccg gaccgagttc gtgagcgagg
     3061 ag
//
LOCUS       DASRWS010000618         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2837060, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000618 DASRWS010000000
VERSION     DASRWS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837060"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFF72_11795"
     CDS             complement(<1..605)
                     /locus_tag="VFF72_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZV93894.1"
                     /translation="MRILDTAEWSRLSPLLDELLDLEPPAQRARLDVLEATDCALADR
                     LRSLLSQRQAVDDEGFLEGDAMPLAIGAPGAGRRIGAYTLESELGAGGMGSVWLARRS
                     DGRFEGVAAVKLPHPGMLAGGADRFAREAALLARLAHPNIATLLDAGVSADGQPYLVL
                     ERVEGEPIDRWCDARNATVDQRLRLFLEVLNAVEHAHQRLVL"
     gene            complement(673..1311)
                     /locus_tag="VFF72_11800"
     CDS             complement(673..1311)
                     /locus_tag="VFF72_11800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZV93895.1"
                     /translation="MTVGADGIEDNPERCDGPASGVADEASDEDAQALSAMFVAAERG
                     DTPATQELFATLYRELHRLARRQLHVGGGALTIGATTLMHEAYIQMSGRGGLTFPQRS
                     RFFAYAARAMRSLIVDYARERRALKRGGAYHLTSGLTEEQVAVPAEDLQPLSDALDAL
                     ARVDAELAELVELKFFGGFDFAEIAEMRHVSTRTVHRHWEKARLLLRHLLAD"
     gene            complement(1455..1531)
                     /locus_tag="VFF72_11805"
     tRNA            complement(1455..1531)
                     /locus_tag="VFF72_11805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1495..1497),aa:Val,seq:gac)
     gene            1626..>2065
                     /locus_tag="VFF72_11810"
     CDS             1626..>2065
                     /locus_tag="VFF72_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HZV93896.1"
                     /translation="MPRAAAAGLLALALSPLAFAEPIGEVDTAFKLIGPDHKIVVEAY
                     DDPKVAGITCYVSRAKTGGIKGALGIAEDKSDASIACRQVGPISIPKPLPQQEEVFSE
                     RLSVLFKRLRIVRMVDRQRNTLVYLTYSDRLVDGSPKNSVTAVP"
BASE COUNT          358 a          719 c          679 g          309 t
ORIGIN      
        1 agaacgagac gctgatgcgc gtgctcgacg gcgttcagca cctcgagaaa gaggcgcagg
       61 cgctgatcga cggttgcgtt gcgggcgtcg caccagcggt cgatcggctc gccttcgacg
      121 cgctcgagca ccaggtaggg ctgcccgtct gccgagacac cggcgtcgag cagcgtcgcg
      181 atgttcggat gcgcgagcct ggccagcagg gcggcctcgc gggcgaagcg atcagcgccg
      241 ccggcgagca tgccggggtg cggcaacttg accgccgcca cgccctcgaa ccggccatcg
      301 ctgcgacgag ccagccagac gcttcccatg ccgccggcgc caagctcgct ttcgagggtg
      361 tatgcgccga tgcgtcgacc ggcgcctggc gcaccgatcg ccagcggcat ggcgtcgcct
      421 tcgagaaagc cttcgtcgtc gactgcctgg cgctgactca gcagcgagcg cagcctgtcg
      481 gcgagtgcgc aatcggttgc ctcgaggaca tcgaggcgcg cgcgctgggc tggcggctcg
      541 agatcgagca gctcatcgag cagcgggctg agccgagacc actcggccgt gtccaggata
      601 cgcatacggg gggctcacga acagtcgatc cctctggaag cggattgtga cgacgtcacc
      661 agcctcgccg agtcaatccg cgagcaagtg gcgcaacagc agtcgcgcct tctcccaatg
      721 gcgatgcacc gtgcgcgtgc tgacgtggcg catctcggcg atctccgcaa aatcgaagcc
      781 gccgaagaac ttgagttcga cgagctcggc aagctcggca tcgacccggg ccagtgcatc
      841 gagcgcatcc gacaacggtt gcagatcttc ggccggaacc gcgacctgct cttcggtgag
      901 gccggaagtc aggtggtagg cgccgccacg cttcaacgcc cggcgctccc gggcgtagtc
      961 gacgatcagg ctgcgcatcg cccgcgccgc ataggcgaag aagcgcgagc gttgcggaaa
     1021 tgtcaggccg ccgcgcccgc tcatctggat ataggcctcg tgcatcagcg tcgtcgcgcc
     1081 gatcgtgagg gcaccgccgc cgacgtgaag ctggcgacgc gccaaacggt gcagctcccg
     1141 atagagcgtc gcgaaaagct cctgcgtcgc cggtgtgtca ccgcgctcgg ccgccacgaa
     1201 catcgccgac agcgcctgcg cgtcctcgtc cgaggcttca tcggccacgc cggacgccgg
     1261 cccgtcgcat cgttcggggt tgtcttcgat cccgtctgca ccgaccgtca tcgaggttct
     1321 gccgcgaaat cggccgagac gagcgcgaag cccggcaatc cggcccggat cgaaggcgat
     1381 ccattcccgc aattccgtgg cgacagaaaa gcccctggcg cttccacgag gtcctttgtg
     1441 ccgtccaccg attctggtag gcgcgattgg actcgaacca acgaccccca ccatgtcaag
     1501 gtggtgctct aaccagctga gctacgcgcc tgcgaaaaac gaatgatagc agcgagtgcg
     1561 catgcgcccg ggcgcggtta gcctcgccga gtcgcgatcc gcgatgaccg gaggacccca
     1621 tgaagatgcc tcgcgccgcc gccgccggac tgctcgccct cgccctgtca ccgctcgcct
     1681 ttgccgagcc gatcggcgag gtcgacacgg cattcaagct gatcggcccc gaccacaaga
     1741 tcgtcgtcga ggcctacgac gacccgaagg tcgcgggcat cacctgctac gtgtcgcgcg
     1801 ccaagaccgg cggcatcaag ggcgcgctgg gcatcgccga ggacaagtcc gacgcgtcca
     1861 tcgcctgccg ccaggtcggc ccgatcagca tccccaagcc gctgccgcag caggaggagg
     1921 tgttcagcga gcgcctgagc gtgctgttca agcggctgcg catcgtgcgc atggtcgacc
     1981 ggcagcgcaa cacgctggtc tacctgacct actccgaccg cctggtcgac gggtcgccga
     2041 agaactcggt gacggccgtg cccat
//
LOCUS       DASRWS010000619         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4256775, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000619 DASRWS010000000
VERSION     DASRWS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4256775"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFF72_11815"
     CDS             complement(<1..255)
                     /locus_tag="VFF72_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZV93897.1"
                     /translation="MALPEPRILGYLRWLAKSRGLEFDVSTVDGYDRLWRWSCDDLRA
                     FWQSIWDWFEVESPVPHDAVLVEETMPGAQWFRGARVNYAG"
     gene            complement(285..>2118)
                     /locus_tag="VFF72_11820"
     CDS             complement(285..>2118)
                     /locus_tag="VFF72_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV93898.1"
                     /translation="TQGVGVKRRAILSSGAALAASPWLAARAAPASAGDRKVLRYAFL
                     AAETSFDPAAIDDLYSRIVTAHLFEAPYRFDYLARSPSKVVPHTAVAMPETADDFRSW
                     TVRIRPGIYFLDDPAFGGKPRELVAADYAYSWKRFFDPATRSPNYSGFNEEGVLGVDA
                     LRQEAIAGKRPFDYDKEVEGLRLLDRYTLQFRLARSRPRFLYTIADSSLYGAVAREVV
                     ERYGARIGEHPVGTGAFRIAEWRRSSFLAFEKNAGYRDVRYDAEPGADDAAGQALLSE
                     FRGRKLPMVDRVEISIIEESQPRWLSFLDGSFDLMSVPLEFANQAVPGGHLAPYLARR
                     GIRVDRFANPDRTLFYFNMTDPIVGGMSPAKVALRRAISLAIDVGAEIRGVRRGQAIP
                     AQSTVAPGGYGYDPAYRSENSVYNVARAKALLDMYGYVDRNGDGWRDMPDGKPLVLEY
                     ASTPDALARQFDELWKKNMDAINVRLKVKTAQWPEELKQAKAGKLMIWELGYTSSSPD
                     FQDGLLLLYGPAAGGQNLSRFEDPRFDELYRRMLGMPDGPERLALLHQADDIITAYAP
                     QKYLVHRIVTYLIQPWLIGYRAPYYGNQFWQYVDIDNGKRHEGK"
BASE COUNT          352 a          695 c          730 g          341 t
ORIGIN      
        1 acccgcgtag ttgacgcggg cgccgcgaaa ccattgcgct ccgggcatcg tctcttccac
       61 cagcaccgcg tcgtgcggaa ccggcgactc gacctcgaac cagtcccaga tcgactgcca
      121 gaacgcacgc aggtcgtcgc acgaccagcg ccagagccgg tcgtagccat cgaccgtcga
      181 gacgtcgaac tcgaggccgc gcgatttcgc gagccagcgg agataaccca ggatgcgggg
      241 ttcgggaagc gccatggccg ggctgcggag ggtggttggg aagactactt gccctcgtgg
      301 cgcttgccgt tgtcgatgtc gacgtactgc cagaactggt tgccgtagta cggcgcgcga
      361 tagccgatca gccagggctg gatcaggtag gtgacgatcc ggtgcacgag atatttctgc
      421 ggcgcgtagg cggtgatgat gtcatcggcc tggtgcagca gcgccagccg ctccggcccg
      481 tcgggcatgc ccagcatgcg tcggtagagc tcgtcgaagc gcgggtcctc gaagcgcgag
      541 aggttctggc cgcctgcggc cggcccgtaa agcagcagca gtccgtcctg gaagtcgggc
      601 gacgacgagg tatagccgag ctcccagatc atgagcttgc cggctttcgc ctgcttcagc
      661 tcttccggcc actgcgcggt cttcaccttc aggcggacgt tgatggcgtc catgttcttc
      721 ttccacagct cgtcgaactg gcgcgccagc gcgtcgggcg tcgaggcgta ctcgagcacc
      781 agcggcttgc cgtcgggcat gtcgcgccag ccgtcgccgt tgcgatcgac gtagccgtac
      841 atgtcgagaa gcgccttggc gcgggcgacg ttgtagacgc tgttctcgct gcgatatgcc
      901 gggtcgtagc cgtagccgcc gggcgcgacc gtcgattgcg ccggaatcgc ctggccacgc
      961 cggacaccac ggatctccgc gccgacatca atggccaggc tgatcgcacg acgcaaggcc
     1021 accttggcgg ggctcatgcc accgacgatc ggatcggtca tgttgaagta gaagagggtc
     1081 cggtcggggt tggcgaagcg gtcgacgcgg atgccccgcc tggccaggta cggcgcgagg
     1141 tggccgcccg gcaccgcctg gttggcgaac tcgagcggca ccgacatcag gtcgaacgag
     1201 ccgtcgagga acgacagcca gcgtggctgg ctttcctcga tgatggagat ctcgacgcga
     1261 tcgaccatcg gcagcttgcg gccgcgaaac tcgctcaaca gcgcctgtcc cgccgcatcg
     1321 tcggcgcccg gctcggcgtc gtagcgcacg tcgcggtagc cggcgttctt ctcgaaggcg
     1381 agaaacgagc ttcgccgcca ctcggcgatg cgaaaggcgc ccgtgccgac cggatgctca
     1441 ccgatgcgcg cgccataacg ctcgaccacc tcgcgcgcca ccgcgccgta gaggctggaa
     1501 tcggcgatgg tgtagagaaa tcgcgggcgc gacctggcga ggcggaactg cagcgtgtag
     1561 cgatcgagca ggcgcagccc ttcgacctcc ttgtcgtagt cgaacggccg cttgccggcg
     1621 atcgcctcct ggcgcagggc atcgacgccg agaacgcctt cctcgttgaa cccgctgtag
     1681 ttagggcttc gcgtcgccgg gtcgaagaac cgcttccagc tgtaggcgta gtcggcggcg
     1741 acgagctcgc gcggcttgcc gccgaaggcc gggtcgtcga gaaaatagat gcccggcctg
     1801 atgcgcaccg tccagctgcg aaagtcgtcg gcggtttcag gcatcgccac ggccgtgtgc
     1861 gggactacct tcgacggcga gcgcgccagg tagtcgaagc gatagggcgc ctcgaagaga
     1921 tgggcggtga cgatgcgcga gtagaggtcg tcgatcgccg ccggatcgaa cgacgtttcg
     1981 gcggcaagga aggcgtagcg cagcaccttc ctgtcgcccg cactcgcagg cgccgcccgc
     2041 gccgcgagcc acggtgacgc ggcaagcgcc gctccgctgg aaagaatcgc ccgccgcttc
     2101 acgccgacgc cttgcgtc
//
LOCUS       DASRWS010000620         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2261404, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000620 DASRWS010000000
VERSION     DASRWS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261404"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFF72_11825"
     CDS             complement(<1..418)
                     /locus_tag="VFF72_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZV93899.1"
                     /translation="MQLYIGNKNYSSWSLRPWLLMTQAGIEFEEKRLRLSSQDDSDFK
                     KTLLAIAPTGRVPLLVDEGFAVWDSLAIAEYLAERFPRKTLWPASLRERARARSLCAE
                     MHSGFADLRGTFGMNLEASLPEVGERMLRERPGTARD"
     gene            complement(546..1505)
                     /locus_tag="VFF72_11830"
     CDS             complement(546..1505)
                     /locus_tag="VFF72_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HZV93900.1"
                     /translation="MPNKFLVLGGTGFVGRSVAEKLVQRSGGADGRLRIATRRFPRAR
                     HLQLLPTVEIACGDVHDEATLARMLRDTHAVINLVAILHGSEAEFERVHVRLPARLAA
                     ACAAAGVRRVIHLSALGAAKDAPSRYQRSKARGEEVLQAAGLDLTILRPSVIFGERDR
                     FLNLFARLQSIFPVLPLASAGARFQPVWVDDVASAVVRAIDDPTTIGTTIECCGPRVY
                     SLEELVRAAGEWSGSQRPILALPAVLGRLQAFFMEMLPGRTLMSRDNVDSMRVANVAS
                     GKLPGLERLGIEPSPLEAIAPRYLGHVSGEKRLEAWRSRARRG"
     gene            complement(1577..>2425)
                     /locus_tag="VFF72_11835"
     CDS             complement(1577..>2425)
                     /locus_tag="VFF72_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZV93901.1"
                     /translation="LNYYGELAAEELGEPFVLPPRPAPLSAAERDAAATRPGLQRALF
                     LIAMGLRSEGVREWNFSIRGFDDRELLAAAQLACDHEVWDRCINTSDRTRGEIDIEQR
                     FPTPHRREVAAAARAAGLDPAFVYGLIRQESRFVMDARSGAGASGLMQLMPGTAKWTA
                     KKLGIPYTPDLIADRETNLALGNAYLKLVLDDVGGSQPMAAAAYNAGPGRPRQWRNGP
                     VLDAAVWAENIPFPETRDYVRKVLSNATYYAAILGTKGETIRARLGPPVGPADPAATP
                     ADKNLP"
BASE COUNT          339 a          829 c          885 g          372 t
ORIGIN      
        1 ggtcgcgcgc agtgccgggc ctctcgcgca gcatgcgctc gccgacttcg ggcaggctcg
       61 cctcgaggtt catgccgaag gtgccgcgca ggtcggcaaa gcccgagtgc atctcggcgc
      121 aaaggctgcg cgcccgcgcc cgctcgcgca gcgacgccgg ccacagcgtc ttgcgcggaa
      181 agcgctctgc gaggtattcg gcgatcgcca ggctgtccca gacggcgaag ccttcgtcga
      241 cgagcagcgg cacgcggccg gtcggcgcga tcgcgagcag cgtcttcttg aagtcggaat
      301 cgtcctgcga ggacagtcgc aggcgcttct cctcgaactc gatgccggct tgcgtcatca
      361 gcagccaggg tcgcagcgac catgacgagt agttcttgtt gccgatgtag agctgcatgc
      421 gcgcctcgtc gtcgtccggg cggctcatgc tagcggagac gcttcagcaa tggctttcgt
      481 atcggtcaag ccggcgatgc gtggcgcgca tcgtttcgcg acagcgcacg accgcgcgac
      541 ccgcttcagc cgcgccgcgc ccgcgatcgc caggcttcga gccgcttctc gcccgagacg
      601 tggccgagat agcgcggcgc gatggcctcg agcggcgacg gttcgatgcc gaggcgttcg
      661 agcccgggca gcttgccact cgcgacgttg gcgacgcgca tcgaatcgac gttgtcgcgc
      721 gacatgagcg tccgccccgg cagcatttcc atgaagaacg cctgcaggcg gccgaggacc
      781 gccgggagcg ccaggatcgg ccgctgcgag cccgaccatt cgccggccgc gcgaaccaat
      841 tcctcgagcg agtagacacg cgggccgcag cactcgatcg tcgtgccgat cgtcgtcgga
      901 tcgtcgatcg cgcgcaccac ggcgctggcg acgtcgtcga cccacaccgg ctggaagcgg
      961 gcgccggcac tggccagcgg caggaccgga aagatcgact gcagtcttgc gaacaggttc
     1021 aggaagcggt cgcgctcacc gaagatcacc gacggccgca ggatcgtcag gtcgagtccg
     1081 gcggcctgca gcacttcctc gccgcgcgct ttcgagcgct ggtagcggct cggcgcatcc
     1141 ttggcggcgc cgagcgcgct caggtggatg acccggcgca cgccggccgc cgcgcacgcg
     1201 gccgcgagcc gggccggcag gcggacgtga acgcgttcga attccgcctc gctgccgtgc
     1261 aggatggcga cgaggttgat caccgcgtgg gtgtcgcgca gcatgcgcgc gagcgtcgct
     1321 tcgtcgtgca cgtcgccgca ggcgatctcg accgtcggca gcaattgcag atggcgggcg
     1381 cgcgggaagc gtcgcgtggc gatgcgcagg cgtccgtcgg cgccgccgga gcgctggacg
     1441 agcttttcgg cgacgctgcg gccgacgaag ccggtgccgc cgaggacgag gaacttgttg
     1501 ggcatgctcg acattcgtgg tgtctccggc gatcccgccg gtcgccggcg attctcgccg
     1561 agcgccgcgg gcacgctcag ggaaggttct tgtcggccgg cgtcgccgcc ggatcggccg
     1621 gccccaccgg cggcccgagc cgggcccgga tcgtttcgcc cttcgtgccg aggatcgccg
     1681 cgtagtaggt ggcgttgctg agcaccttgc ggacgtagtc ccgcgtttcg gggaagggaa
     1741 tgttctcggc ccacactgcc gcgtcgagca cggggccgtt gcgccactgg cgaggccggc
     1801 cggggccggc gttgtaggcg gccgcggcca tgggctgcga tccgccgacg tcgtcgagca
     1861 cgagcttcag gtaggcgttg ccgagcgcga ggttggtctc ccggtcggcg atgaggtcgg
     1921 gcgtgtacgg gatgccgagc tttttcgccg tccacttcgc ggtgccgggc atgagctgca
     1981 tcaggccgga cgcgccggcg cccgagcgcg cgtccatgac gaagcgtgat tcctgccgga
     2041 tcagcccata gacgaaggcc gggtcgaggc cggcggcgcg cgccgcggcg gcgacctcgc
     2101 gccggtgcgg cgtcggaaat cgctgctcga tgtcgatttc gccgcgcgtg cggtcgctgg
     2161 tgttgatgca gcgatcccag acttcgtgat cgcacgccag ttgcgccgcg gccagcagct
     2221 cgcggtcgtc gaagccgcgg atcgagaaat tccattcgcg cacgccttcg ctgcgcagtc
     2281 ccatcgcgat caggaacaag gcccgctgca gaccgggccg cgtcgccgcc gcgtcgcgtt
     2341 cggccgcgct gagcggcgcc ggccgcggcg gcaggacgaa cggttcgccg agctcttcgg
     2401 cggcgagctc gccgtaatag ttcag
//
LOCUS       DASRWS010000621         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3980446, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000621 DASRWS010000000
VERSION     DASRWS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3980446"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFF72_11840"
     CDS             <1..543
                     /locus_tag="VFF72_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93902.1"
                     /translation="MAQLRAQPLVGVVLADNPAIEAQLRAAVEADQRDPQATSRAFLA
                     VSEMRRTIVGPALAAADDASALAVMKARVDLVSHLEKTDLAACRQFSGDGIRDVNKLD
                     DEGQRLFHAMLTTMEAAYRSGKANGKGKPPTNEEFAAMLFVPSIGGISHHFSENTADT
                     DIVLGCQVFADATAKILKGN"
     gene            complement(553..1254)
                     /locus_tag="VFF72_11845"
     CDS             complement(553..1254)
                     /locus_tag="VFF72_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93903.1"
                     /translation="MKRNTTIVIAVLAAGIIAAAIAAWAVKSFTGDSATDKTMAQLRA
                     QPLVGVVLADNPAIEARLRADVEEDQRNPQATPRAFLAISEMRRTIVAPTLSAADDAS
                     ALAVMKARADLVAYLEKTDLAACRQFSGDGIRDINKLDSEGQTQFRTMLTAMEAAYRS
                     GKANGGKGKSVSNAEFAEMLRTAGFTPDDFAKFSQTATLKDDELCNLEFRIDDAPSKL
                     PDDKRGAFARFVIAH"
     gene            complement(1292..1579)
                     /locus_tag="VFF72_11850"
     CDS             complement(1292..1579)
                     /locus_tag="VFF72_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HZV93904.1"
                     /translation="MGARHRAKLKKWSGKVTRESDALDLEHGVFKKRSAHDIAVSLKR
                     SAERSRRRKSAPLRSAMSMLTFYINRAGKRLSASRRRTLEKAKDELRKLFH"
     gene            complement(1647..2375)
                     /locus_tag="VFF72_11855"
     CDS             complement(1647..2375)
                     /locus_tag="VFF72_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93905.1"
                     /translation="MPIEIDRRDLYRWLATGLLTLVCLVAIVGGGWALVRGKSPVDRV
                     LDDVRRLPLIGTVMADNAGAESRMRKAIEEELRSPTRAGLSRPLSVVADLRREYIVPA
                     LRAADDASALAALGARADFARYLRRADPGACRQFALGILLRPDLLQSEGRQLFAEYLQ
                     ALLAAYRNGKAAGKPQPFLTRDELTSALRQAGFTKMDFDRLQGFQSLSNEVSCDVEIK
                     VDGASERLPAQMRGPYARYVLTSN"
BASE COUNT          374 a          830 c          890 g          406 t
ORIGIN      
        1 atggcccagc tgcgcgccca gccgctggtc ggcgtggtgc tggccgacaa tccggcgatc
       61 gaggcgcaac tgcgcgctgc cgtcgaggcg gaccagcgcg acccgcaggc gacgtcgcgg
      121 gccttcctcg ccgtcagcga gatgcgccgc accatcgtcg gtcccgccct tgccgcagcc
      181 gacgatgcga gcgcgctcgc cgtgatgaag gcgcgcgtcg acctggtgag ccatctggag
      241 aagaccgatc tcgccgcctg ccgccagttt tccggcgacg gcatccgcga cgtcaacaag
      301 ctcgacgacg agggccagcg gctgttccat gccatgctga cgacgatgga agcggcctat
      361 cgcagcggca aggcgaacgg caagggcaag ccgccgacga atgaggagtt cgcggcgatg
      421 ctgttcgtgc cgtcgatcgg cggtatctcg catcatttca gcgagaacac cgccgacacc
      481 gacatcgtgc tgggctgtca ggtcttcgcc gacgccacgg cgaagatcct caaggggaac
      541 tgaagccgga agtcagtggg cgatcacgaa gcgggcgaag gcgccgcgct tgtcgtcggg
      601 cagcttgctc ggggcgtcgt cgatccggaa ctcgaggttg cagagctcgt cgtccttcag
      661 cgtcgcggtc tggctgaact tggcgaagtc gtccggggtg aagcccgcgg tccgcagcat
      721 ctcggcgaac tccgcgttgc tcacggactt gcccttgccg ccattcgcct tgccgctgcg
      781 ataggccgct tccatcgccg tcagcatggt gcggaactgc gtctggcctt cgctgtcgag
      841 cttgttgatg tcgcgtatgc cgtcgccgga gaactgccgg caggcggcga gatcggtctt
      901 ctcgagatag gccaccaggt cggcgcgcgc cttcatcacc gcgagcgcgc tcgcatcgtc
      961 cgcggcggag agggtgggag cgacgatggt tcgccgcatc tcgctgatgg cgaggaaggc
     1021 ccgcggcgtg gcctgcgggt tgcgctggtc ctcctcgacg tcggcgcgca gccgcgcctc
     1081 gattgccggg ttgtcggcca gcaccacgcc gaccagcggc tgggcgcgca gctgggccat
     1141 ggtcttgtcg gtggcggagt cgccggtgaa cgacttcacg gcccaggcgg cgatggccgc
     1201 ggcgatgatg cctgcagcca gcacggcgat cacgatggtg gtgttgcgct tcatggatcc
     1261 ccgcttcggc gcgagggtaa caggacgcgc actagtggaa cagcttgcgc agctcgtcct
     1321 tggccttttc gagcgtgcgg cggcggctcg ccgacaatcg cttgccggcg cggttgatgt
     1381 agaaggtcag catcgacatc gccgaccgca gcggcgccga cttgcgccgc cggctgcgct
     1441 cggccgagcg cttcagcgac accgcgatgt cgtgcgcgct gcgcttcttg aagacgccgt
     1501 gctcgagatc gagcgcatcg ctttcgcgcg tcaccttgcc tgaccatttc ttgagcttgg
     1561 cacggtgccg cgcgcccatg cgggcatgcc cgccatgtgg tgtcgctgcc atgatgcgac
     1621 aacgcccgga cgtgccggtt cgttgctcag ttgcttgtca ggacgtagcg cgcatagggc
     1681 ccgcgcattt gggcgggcag acgctccgat gcgccgtcga ccttgatctc gacgtcgcag
     1741 ctcacttcgt tggacaggct ctggaacccc tgcagccggt cgaaatccat cttcgtgaaa
     1801 cccgcctgcc tgagcgcgga ggtgagctcg tcgcgggtca ggaagggttg cggcttgccg
     1861 gccgccttgc cgttgcgata ggcggccagc agcgcctgca ggtactcggc gaacagctgc
     1921 cggccctccg actgcagcag gtcgggccgc agcaggatgc cgagcgcgaa ctggcggcat
     1981 gcgccggggt cggcgcgccg caggtagcgc gcgaagtcgg cgcgcgcgcc gagcgccgcc
     2041 agcgcactgg cgtcgtcggc ggcgcgcagc gccggcacga tgtactcgcg tcgcaggtcg
     2101 gcgacgaccg agagcgggcg cgacaggccg gcccgggtcg ggctgcgcag ctcctcctcg
     2161 atcgccttgc gcatgcggct ttcggcgccg gcattgtcgg ccatcaccgt gccgatcagc
     2221 ggcaggcggc ggacgtcgtc gagcacccgg tcgaccgggc tcttgccgcg caccagtgcc
     2281 cagccgccgc cgacgatcgc caccaggcac acgagggtga gcaggccggt ggcgagccag
     2341 cgatagaggt cgcggcggtc gatctcgatc ggcatggttc agcgccgcat gcgtcaggga
     2401 tggatgacca tgccggtgaa gacataggcc tgcagcatca cgaatacgcc gaccaggcag
     2461 gcgagcgcga tcgagtgctt gaagacgaag cgcaggatcg
//
LOCUS       DASRWS010000622         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_823502, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000622 DASRWS010000000
VERSION     DASRWS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823502"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="asd"
                     /locus_tag="VFF72_11860"
     CDS             complement(<1..661)
                     /gene="asd"
                     /locus_tag="VFF72_11860"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746858.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZV93906.1"
                     /translation="MSDRRKVLPQYVLPKRGLTAFAGHVASGERGASTTRLVRWFVRK
                     YAVDMSEAAEPDIAAYASFNDFFTRALRPGARPIADAALVCPVDGTISQLGAIEHDQI
                     LQAKGHRYSTTALVGGDAALAAAFHDGSFATLYLSPKDYHRIHMPCDGVLTRMIHVPG
                     SLFSVNPVTARGVPRLFARNERVVCVFESDVFGVFVLALVGATIVGSMATVWHGVVNP
                     PR"
     gene            821..1417
                     /locus_tag="VFF72_11865"
     CDS             821..1417
                     /locus_tag="VFF72_11865"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZV93907.1"
                     /translation="MSRIDFHASSVEVARALIGASVFVEGVGGRIVETEAYDESDPAS
                     HTFAGRTARNAVMFGPPGRAYVYRSYGLHWCLNFACMPSGHGAGVLIRALEPLAGIDV
                     MRERRGVEPLRLLCSGPGRLCQALAVTHAMNGLRLDRAPFRVVATAAAEKVEVAAGVR
                     IGIRKAAETPWRFVLVGSRFLSKPLRGAVSSTAAPAGR"
     gene            complement(1485..2642)
                     /locus_tag="VFF72_11870"
     CDS             complement(1485..2642)
                     /locus_tag="VFF72_11870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZV93908.1"
                     /translation="METIPRRADEFDVIVVGGGIAGASLAYFLAPHARVVVLERESQP
                     GYHSTGRSAAMFMESYGTAQVRSLTLASRAFLEAPPEGFADGPILSPRGAMMVAAPGQ
                     DDALEAQWAVLQATSPGGRRLGVDAACARVPVLRRERVLGAVFEPDASDIDVARLHQG
                     YLRGTRRAGGRIVSDAEVTSLRIASGGDGWLVEAGADRYAAPIVVDAAGAWSDVVARR
                     AGVAPIGLVPKRRSAFVFAPPAGSDVASWPMFAGIDESWYVKPDATHLLGSPANVDPV
                     EPQDVQPEELDIALAVHRIEEATTLVARPLRRWAGLRSFVADGDLVGGFDPQAPGFFW
                     LAAQGGYGIQTSAAMGEACAALVRGLPLPKRIADFGLDAHRLGPARFATRR"
     gene            2797..3576
                     /locus_tag="VFF72_11875"
     CDS             2797..3576
                     /locus_tag="VFF72_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV93909.1"
                     /translation="MRSRSLRLRSLRWAGFVLVGVVAACGAAAAERVQLVAADQVAVF
                     GEVWRAAGERPPVIIAFHQAESNHAEYAPIAPRLAQDGFTVLAIDQRSGDGAFGGTNL
                     TVETLGDSTDYDEALPDLEAALAWAHEHADGAPVIAWGSSYSAALVFLLAARHADEVS
                     AVVAFSPGEYLRNPTAVHDAARQLTMPVYIDQASSADEIAASTSILDAVGNSHKRMFM
                     QDPSTHGASTLRADSNPMGNEAHWQSVLKFLAPFKHEETKR"
BASE COUNT          517 a         1320 c         1378 g          542 t
ORIGIN      
        1 cccgcggcgg gttgacgacg ccgtgccaga ccgtcgccat gctgccgacg atcgtcgcac
       61 cgacgagggc gaggacgaag acgccgaaga cgtccgactc gaagacgcag acgacgcgct
      121 cgttgcgagc gaacaggcgc ggcacgccgc gcgcggtcac cggattcacc gagaacagcg
      181 accccggcac gtggatcatt cgcgtcagca ccccgtcgca cggcatgtgg atgcggtggt
      241 aatccttcgg gctcaggtag agcgtcgcga agctgccgtc gtgaaaggcc gccgccagcg
      301 ccgcgtcgcc gccgaccagc gcggtcgtcg agtagcggtg gcccttggcc tgcaggatct
      361 ggtcgtgctc gatcgcgccg agctggctga tcgtgccgtc gaccgggcag acgagcgcgg
      421 catcggcgat cggccgcgcc ccgggcctga gggcgcgcgt gaagaagtcg ttgaagctgg
      481 cgtaggcggc gatgtcgggc tcggccgcct cgctcatgtc gaccgcgtac ttgcgaacga
      541 accagcgcac cagccgggtc gtgctcgcgc cgcgctcgcc cgacgcgacg tggcccgcga
      601 acgcggtgag gccgcgcttc ggcagaacgt actgcggcag gaccttgcgg cgatcggaca
      661 cgaagcggcg gataggcgaa gagtggggcg tcggaattct aggggccgct gtcgcgcggc
      721 tcgggcgcgg cgactcgtac gcccctgccg cgaatcaccg ggcgaccacg acgacgtctc
      781 gtcgcggtct cgccgcggcg cagcccgata tcctcgccgc atgtcgcgca tcgatttcca
      841 cgcgtcgtcg gtcgaggtcg cccgggcgct gatcggcgcc agcgtcttcg tcgaaggcgt
      901 cggcggtcga atcgtcgaga ccgaggccta cgacgagagc gatccggcgt cgcacacctt
      961 cgccggccgg acagcgcgca acgcggtgat gttcgggccg ccggggcgcg cctacgtcta
     1021 tcgctcctac ggtctgcact ggtgcctgaa tttcgcgtgc atgccgagcg gccacggcgc
     1081 cggggtgctg atccgggcgc tcgagccgct cgccggcatt gacgtgatgc gcgagcgacg
     1141 cggcgtcgag ccgctgcgcc tgctctgctc ggggccggga cggctgtgcc aggccctggc
     1201 ggtgacccac gcgatgaacg gtcttcgcct cgaccgggcg ccgtttcgcg tcgtcgcgac
     1261 cgctgcagcg gagaaggtcg aggtggctgc cggcgtccgc atcggtatcc gcaaggcggc
     1321 cgagacgccg tggcgcttcg tgctcgtcgg gtcgcgcttt ctcagcaagc cgctgcgcgg
     1381 cgccgtgtcg tcgaccgcgg cgccggcggg ccgctgatcg agcgagatcc gcgtggcggc
     1441 gacggccggc cgctgatcgg acgaagtccg ggcggcgtcg ggcttcagcg cctggtggcg
     1501 aaccgggccg gcccgagcct gtgggcgtcg aggccgaagt cggcgatgcg tttgggcagc
     1561 ggcaggccgc gaaccagcgc cgcgcaggct tcgcccatcg ccgccgaggt ctggatgccg
     1621 tagccgcctt gcgcggcgag ccagaaaaag cccggcgcct gcgggtcgaa gccgccgacc
     1681 agatcgccgt cggcgacgaa cgagcgcagg cccgcccacc ggcgcagcgg tcgggcgacg
     1741 agcgtcgtcg cttcctcgat ccggtgcacg gccagcgcga tgtcgagctc ctcgggctgc
     1801 acgtcctgcg gctcgaccgg atcgacgttg gccggcgagc cgagcaggtg cgtcgcatcg
     1861 ggcttgacgt accagctttc gtcgatgccg gcgaacatcg gccaggacgc cacgtcgctg
     1921 cctgcgggcg gcgcgaagac gaaggcgctg cgtcgcttcg ggacgaggcc gatcggcgcg
     1981 acaccggccc gtcgcgcgac gacgtcggac caggcgccgg cggcgtcgac gacgatgggc
     2041 gctgcatagc ggtcggcgcc ggcctcgacg agccagccgt caccaccgct cgcgatgcga
     2101 agggaggtga cctcggcgtc gctgacgatg cggccgccgg cgcggcgcgt gccacgcagg
     2161 tagccctggt gcagccgggc gacatcgatg tcggatgcgt cgggctcgaa gacggcaccg
     2221 agtacccgct cgcggcgcag caccggcacg cgagcgcagg cggcgtcgac gcccagccgt
     2281 cggccgcccg ggctcgtcgc ctgcagcacc gcccactgcg cctcgagcgc gtcgtcctgg
     2341 ccgggagcgg cgaccatcat ggcgccgcgc ggcgagagga tggggccgtc ggcgaagcct
     2401 tcgggcggtg cctcgagaaa ggcccggctg gccagcgtca gcgaccgcac ctgggccgtg
     2461 ccgtagctct ccatgaacat cgccgccgaa cgaccggtcg agtggtagcc gggctggctt
     2521 tcgcgctcga ggacgacgac gcgcgcgtgc ggtgccagga agtaggcgag cgacgcgccg
     2581 gcgatgccgc cgccgacgac gatgacgtcg aattcgtcgg cccgtcgagg gatcgtttcc
     2641 atgctgtttc gtccgcgcct cttccgctcg ggtgcgcgat gtcctgcatg ccagggtcgg
     2701 cacgttgtcg attgtggccg gcacggatcg gcgagctgcg cagcctgtta gtctttgcac
     2761 ccgtcgcgcc ttcggcgccg tgcgctcctg tcgtccatgc gctctcgatc cctgcgtctt
     2821 cgttcgctgc ggtgggccgg attcgtcctc gtcggcgtcg tcgctgcttg cggcgccgct
     2881 gcagccgagc gggtgcagct ggtcgccgct gaccaggtgg ccgtcttcgg cgaagtctgg
     2941 cgggccgccg gcgagcggcc gccggtgatc atcgcgtttc accaggccga atcgaaccac
     3001 gccgagtacg cgccgatcgc gccgcgcctg gcccaggacg gtttcaccgt gctcgcgatc
     3061 gaccagcgct cgggcgacgg tgccttcggc ggcaccaacc tgacggtcga aacgcttggc
     3121 gactccaccg actacgacga ggcgctcccc gacctcgagg cggccctcgc ctgggcccac
     3181 gaacacgccg acggcgcgcc ggtcatcgcc tggggatcga gctattcggc ggcgctcgtc
     3241 ttcctgctcg ccgcccgcca tgccgacgag gtgagcgcgg tcgtcgcctt ctcgcccggc
     3301 gaatacctgc gcaatccgac ggcggtgcac gacgccgccc ggcagctgac gatgccggtc
     3361 tacatcgacc aggcatcgag cgccgacgag attgccgcgt cgacatcgat cctcgacgcg
     3421 gtaggcaact cgcacaagcg gatgttcatg caggacccct cgacgcacgg cgcgtcgacc
     3481 ttgcgcgccg acagcaatcc gatgggcaac gaggcgcact ggcaatcggt cctgaaattc
     3541 ctcgcgccgt tcaagcacga ggaaacgaag cggtgagaca gacgatgcat tcgacgagtg
     3601 gggcctgcgc ccgcggccca gcgcgtgcgg cgggcgcgac ggggccgtgc tgatgaccgc
     3661 gccgctgcat ccctcgctcg ggctgacgct gggcctcggc gcgttcatcg cctggcgaat
     3721 gatctggcgg gtgcgccgga tggtcggccg tcagcgt
//
LOCUS       DASRWS010000623         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3981797, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000623 DASRWS010000000
VERSION     DASRWS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981797"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFF72_11880"
     CDS             <1..728
                     /locus_tag="VFF72_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HZV93910.1"
                     /translation="LHTVESDAQGGLSVGEVDVFVGPNFVLSVRNRTHAGFLGVRARA
                     EREPQLLRHGPAFVFYALLDAVVDRYFPTIDALETELESIEEQIFEPHLGRVNIERLY
                     ALKRKIGILKHAVAPLQEAVAKLVSGRVHQICNNNREYFRDVFDHLARMNALLDTLRD
                     TIGTAIQVNLASVTIEETEITKRLAAWAGIFAVATAFAGIWGMNFQNMPELKLPWGYP
                     AALLVIALACVFMWWRFRKARWL"
     gene            853..>3049
                     /locus_tag="VFF72_11885"
     CDS             853..>3049
                     /locus_tag="VFF72_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZV93911.1"
                     /translation="MLAAARDAKAAGVDAVAGIIETHGRADTTAQLQGLEVLPRRPAQ
                     AGRGHPEFDLDAALARRPALILVDELAHSNVAGSRHPKRWQDVVELLAAGIDVWTTLN
                     VQHLETLNDVVGGITGIRVGETVPDTVFDSADEVVLVDVPAEELIARLEAGKVYAPGQ
                     AERAAANFFRRGNLMALRELALRRTADRVENDVQAYRVDRSIGRVWKTGSAVLCCVGP
                     SDAAGRVVRSAALLAQQLAVPWHAVYVETPALQRLANERRERILRVLRLAEELGATTA
                     VLAAPRVAAAIVEHARRHNLSKIVTGHAQARRWRPDGSLARQLGRLAPDVDLIEVGAP
                     AHPSRPAASMQRQDARDGRRRLGGYVVAVVACAVLTAAALPLEAILDLPNIVMLFLLA
                     VLGVALRYGRGPAALASVLNVVAFDFFFVPPRLSLGVADLQYVLTFGVMLAVGLIVGQ
                     LTAGLRFQARIATHRERRSHSLFEVARDLSGVLGTEQVVEAAQAAVAREFRARVHIFV
                     LDANDRLVASPGEGDGLDAATARWAFDHGQPAGLGTDTLAGSAWLYLPLKAAMRTRGV
                     LALMPEEPRLLLVPEQRRQLETFAALAAIALERVHYVEVAQAATLQIESERLRNSLLA
                     ALSHDLRTPLAGLVGLAESLNLTQPRLSGEQAEIADELRLEAMRMSTQVGNLLDMARI
                     ESGQVRLRRDWHSVEELVGSAVRATARILAPRRVETDLAQDLPLVECDAV"
BASE COUNT          422 a         1109 c         1057 g          461 t
ORIGIN      
        1 tgctgcatac cgtcgagtcg gacgcccagg gcgggctgag cgtcggcgag gtcgatgtct
       61 tcgtcggccc caacttcgtc ctgtcggtcc gcaaccgcac ccacgccggc tttctcggcg
      121 ttcgcgcgcg ggccgagcgc gagccgcaac tgctcaggca tggcccggcg ttcgtcttct
      181 acgcgctcct cgacgccgtc gtcgaccgct acttcccgac catcgacgcg ctcgagaccg
      241 agctcgagtc gatcgaggaa cagatcttcg agccgcacct cggccgcgtc aacatcgaga
      301 ggctctacgc gctgaaacga aagatcggca tcctcaagca tgccgtcgcg ccgctgcagg
      361 aggccgtcgc caagctggtg agcgggcgcg tccatcagat ctgcaacaac aaccgcgagt
      421 acttccgcga cgtcttcgat cacctggcgc gaatgaacgc gctgctcgac accttgcgcg
      481 acacgatcgg cacggcgatc caggtcaacc tcgccagcgt gacgatcgag gagaccgaaa
      541 tcaccaagcg cctcgccgcc tgggcgggga tcttcgcggt cgccaccgcg ttcgccggca
      601 tctggggaat gaatttccag aacatgcccg agctgaagct gccctggggc tatccagccg
      661 cgctcctcgt catcgcgctg gcctgcgtct tcatgtggtg gcgattccgc aaggccaggt
      721 ggctgtgaac gaacccgcgc ggcccgatcc cgacgcgctc gtcgaacgca ttcgcgacga
      781 cgaggcgcgc cgcagcaggg tcgcctgcgc atctatttcg gctcgtcggc gggcgtcggc
      841 aagacgttcg ccatgctcgc cgcggcgcgc gacgcgaagg cggcgggcgt cgatgcagtc
      901 gccgggatca tcgagacgca cggccgtgcc gacacgacgg cacagctgca gggcctcgag
      961 gtgctgccac gcaggccggc gcaggccggg cgcggccatc cggaattcga cctcgacgcc
     1021 gcgctggcgc gccggccggc gctcatcctc gtcgacgagc tcgcgcattc gaacgtcgcg
     1081 ggctcgcgcc accccaagcg ctggcaggac gtcgtcgagc tgctcgccgc cggcatcgac
     1141 gtctggacga cgctcaacgt ccagcacctg gagacgctca acgacgtcgt cggcggaatc
     1201 accggcatcc gcgtcggcga gacggtgccc gacacggtct tcgacagcgc cgacgaggtc
     1261 gtgctggtcg acgtgccggc cgaggagctg atcgcccgcc tcgaggccgg caaggtctac
     1321 gcccccggcc aggccgaacg cgcggcggcg aacttctttc gccgcggcaa cctgatggcg
     1381 ttgcgcgagc tggcgctgcg tcgcaccgcc gatcgcgtcg agaacgatgt ccaggcctat
     1441 cgcgtcgatc gctcgatcgg ccgggtctgg aagaccggga gcgcggtgct gtgctgcgtc
     1501 ggtccgtccg atgccgccgg gcgcgtcgtc cggagcgccg cgctgctggc gcagcaactg
     1561 gcggtgccat ggcacgccgt ctatgtcgag acgccggcac tgcagcggct tgccaacgag
     1621 cgccgcgaac gcatcctgcg cgtccttcgc ctggccgaag agctcggcgc gacgacggcg
     1681 gtgctcgccg cgccgcgcgt cgccgccgcg atcgtcgagc acgcgcgccg tcacaacctg
     1741 tcgaagatcg tgacaggcca cgcccaggcg aggcggtggc gaccggacgg ctcgctggcg
     1801 cggcaactgg gccggctcgc tcccgacgtc gacctgatcg aggtcggggc gccggcgcat
     1861 ccgtcgcgcc ccgcagcgtc gatgcagcgc caggacgcac gcgacggccg gcggcgcctc
     1921 ggcggctacg tggtcgcggt cgtcgcctgc gcggtgctga ccgccgccgc cttgccgctg
     1981 gaggcgatcc tcgatctgcc caacatcgtc atgctcttcc tgctcgccgt cctcggagtg
     2041 gcgcttcgct acggtcgcgg cccggcggcg ctcgcctcgg tgctcaacgt cgtcgccttc
     2101 gacttcttct tcgtgccgcc ccgcctgtcg ctcggcgttg ccgacctgca atacgtgctc
     2161 accttcggcg tgatgctcgc ggtcggcctg atcgtcggcc agctcaccgc gggcctgcgc
     2221 ttccaggcgc gcatcgcgac gcatcgcgag cgtcgctcgc attcgctctt cgaagtggcc
     2281 cgcgacctgt ccggcgttct cggcaccgag caggtcgtcg aggcggcgca ggccgccgtc
     2341 gcccgcgagt tccgggcccg cgtccacatc ttcgtgctcg acgccaatga ccggctcgtc
     2401 gcttcgccag gggagggcga cggcctcgac gccgcgacgg cgcgctgggc gttcgaccac
     2461 ggccagccgg ccgggctggg caccgacacg cttgcgggca gcgcctggct ctacctgccg
     2521 ctgaaagcgg cgatgcgcac gcgcggcgtc cttgcgctga tgcccgagga accgcgcctg
     2581 ctgctcgtgc ccgagcagcg gcgccagctc gagaccttcg cggcgctggc ggcgatcgcc
     2641 ctcgagcgcg tccactacgt cgaggtcgcg caggcggcga cgctgcagat cgagtccgag
     2701 cggctgcgca attcgctgct cgccgccttg tcgcatgacc tgcgcacgcc gctcgccggc
     2761 ctggtgggac tggcggagag cctgaatctg acgcagcctc gcttgagcgg cgagcaggcg
     2821 gagatcgccg acgagttgcg gctcgaggcg atgcgaatga gcacgcaggt cggcaacctg
     2881 ctcgacatgg cgcggatcga gagcggccaa gtgcggctgc ggcgcgactg gcattcggtc
     2941 gaggagctgg tgggcagcgc ggtgcgggcg acggcgcgca tcctggcgcc gcgccgcgtc
     3001 gagaccgatc ttgcgcaaga cctgccgctc gtcgaatgcg acgccgtgc
//
LOCUS       DASRWS010000624         4478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2265148, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000624 DASRWS010000000
VERSION     DASRWS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2265148"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFF72_11890"
     CDS             complement(<1..1163)
                     /locus_tag="VFF72_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZV93912.1"
                     /translation="MREPCCAPRGATSIVAFAAVVFGLGGCASLIPAYQRPAPPVAPS
                     YAPEMTPAASADSVPAADIEWQRFYAGDPRLERLIGLAIANNRDLRVAILNIEQARAN
                     YQIRRADQLPSVGVGATAQRAPNAFGKLGQTYAVGLSLSSYELDFFGRVHDLSQAALD
                     QFLATTESRKTVQMSLVASVATTYLALLADDELVKVTEETLKTREESTRLTKLRFDNG
                     ASSELDYRQAESLLEGARATLAQSLRQRALDANALTLLVGQTIPADLPPPVTLAGQHL
                     LADLPAGLPADLLERRPDIRAAEQQLLAANANIGAARAAFFPHISLTALAGTASTELS
                     GLFKGGSFAVTGTAQLLQPIFDAGRNQANLDVAKANRDIAVAQYEKSIQTAFR"
     gene            complement(1156..4350)
                     /locus_tag="VFF72_11895"
     CDS             complement(1156..4350)
                     /locus_tag="VFF72_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZV93913.1"
                     /translation="MPQFFIDRPIFAWVIALFILVFGGVAIKQLPIAQYPTVAPPAIV
                     LSATYPGASAQTLEDSVISVIEQEMNGSPGLIYLESVSQSNGTGQITLTYETGTNPDL
                     AQVDVQNRLSRAAPRLPAAVTQQGVRVDKARSNFLLFTILSSSDGSLDPIALGDYASR
                     TVLPEIQRIPGVGQAQLFGTERAMRIWVDPAKLVGYNMSTNDVVAAIRAQNAQVSSGT
                     IGDLPIVPGTPITATVVVAGQLTTVEEFRQIVLRANTDGSTVKLGDVARVELGGQNYG
                     TSARLNGKPSTGIGVQLSPTGNALQTAELIKKRMAELARYFPKGVQWSIPYDSSRFIK
                     ISIGRVVETLVEAVILVFLVMYLFLQSLRYTLIPTIVVPVALFGAFGIMQTFGFSINV
                     LTMFAMVLAVGILVDDAIVVIENVERIMSEEGLSPRDATKKAMGQITAALVGISVTLV
                     SVFLPMAFFSGAVGNIYRQFSLVMVSSMLFSVFLAMSLTPALCATFLKPLEKGAKHEK
                     RGPFGWFNRGFKATTHGYEGWVARLLRHTGPMLVAFVVVVALVALFFTRLPGSFLPTE
                     DQGYLIANVQLPPGATKTRTLAVMEQAEKFFLSQPEVDQMVSVLGFSFSGSGQNAGLA
                     FVTLKPWDEREGTEHSATAIAGRAFGALSKLRDAFIFALVPPAIPELGTATGFAFRLE
                     DRGGNGHAALLAARNQLLGLASKSKVLAGVRPDGLEDAPQLQLDIDRDRANALGVSFD
                     NINAVLSTALGSAYVNDFPNAGRLQRVIVQADAAYRMEPQDLLELNVLNARGQPVPMS
                     ALATTRWITGPIQTIRFNGYPAMRIAGDAAPGYSTGDAMDEMERLAGELPAGFAYEWT
                     GQSREERLSGSTATILIAFSLLAVFLCLSALYESWSIPFAVLLAVPLGVLGAVIGATL
                     RGMPNDVFFKVGLITVIGLSAKNAILIIEFAKDLYAQGKGLVEATLEAVHLRFRPILM
                     TSIAFILGVLPLVISTGAGSASQRAIGTGVMAGMISATLLAIFFVPVFFVVVRRIFKG
                     RRGLPGSPRHGEPATDGTMPAPPSAPTGEEGGHA"
BASE COUNT          730 a         1462 c         1623 g          663 t
ORIGIN      
        1 tcgcgaaacg ccgtctggat cgacttctcg tactgcgcca cggcaatgtc gcgattcgcc
       61 ttggccacgt cgaggttggc ctggttgcgg ccggcgtcga agatcggctg cagcagttgc
      121 gccgtcccgg taacggcgaa gctgccgccc ttgaagaggc ccgagagctc ggtgcttgcc
      181 gtgccggcca gcgccgtcaa cgagatgtgc ggaaagaagg ccgcgcgcgc cgcgccgata
      241 ttggcgttgg cggcgaggag ctgctgctcg gcggcgcgga tgtcgggacg gcgctcgagc
      301 aggtcggccg gcagccccgc aggcagatcg gcgagcagat gctggccggc cagcgtcacc
      361 ggcggcggca ggtcggcggg aatcgtctgg cccaccagca gggtgagcgc gttggcgtcg
      421 agagcgcgct ggcgcagcga ctgggccagc gtggcacggg cgccttcgag caacgactcg
      481 gcctgccggt agtcgagctc cgacgaagcg ccgttgtcga agcgcagctt ggtgaggcgg
      541 gtcgactcct cgcgggtctt cagcgtctcc tccgtcacct tgaccagctc gtcgtcggcg
      601 agcagcgcca ggtatgtcgt cgccaccgag gcgacgagac tcatctgcac cgtctttctc
      661 gactcggtgg tggccaggaa ctggtcgagc gcggcctggc tcaggtcgtg cacgcgaccg
      721 aagaagtcga gctcgtagct cgagagcgac aggccgaccg cgtaggtttg gccgagcttg
      781 ccgaaggcat tcggcgcccg ctgcgccgtc gcaccgacgc cgacgctcgg caactggtcg
      841 gcacgccgga tctggtagtt ggcgcgcgcc tgctcgatgt tgaggatggc gacgcgcagg
      901 tcgcggttgt tcgcgatggc caggccgatg aggcgctcga gccgcggatc gccggcgtag
      961 aagcgctgcc attcgatgtc ggcagccggc acgctgtcgg cagacgcggc tggcgtcatc
     1021 tcgggtgcat agctcggcgc cacgggcggc gccgggcgct ggtaggcggg gatcagcgag
     1081 gcacaaccgc ccaggccgaa gaccaccgcc gcgaaggcga cgatcgatgt cgcgccacgc
     1141 ggcgcgcagc acggctcacg catggccacc ctcctccccc gtcggcgcag acggcggcgc
     1201 gggcatcgtg ccatccgttg ccggctcgcc gtgtcgcggc gaaccgggca ggccacgccg
     1261 gcccttgaag atgcggcgaa cgacgacgaa gaacaccggc acgaagaaga tcgccaggag
     1321 cgtcgccgag atcatgccgg ccatcacgcc ggtgccgatg gcacgctggc tggccgagcc
     1381 cgcaccggtg gagatgacga gcggcagcac gccgaggatg aaggcgatcg aggtcatcag
     1441 gatcggcctg aaccgcaggt gcacggcctc gagcgtcgcc tcgacgagcc ccttgccctg
     1501 ggcgtagagg tccttggcga actcgatgat gaggatcgcg ttcttcgccg acaggccgat
     1561 cacggtgatg aggccgacct tgaagaagac gtcgttgggc atgccgcgaa gcgtcgcgcc
     1621 gatgaccgcg ccgagcacgc cgagcggcac cgccagcagc accgcgaacg ggatcgacca
     1681 gctctcgtag agcgccgaca gacagaggaa gacggcgagc agcgagaagg cgatcaggat
     1741 cgtcgccgtc gagcccgaga gacgctcttc gcgcgactgg cccgtccact cgtacgcgaa
     1801 tccggcgggc agctcgccgg ccaggcgttc catctcgtcc atcgcgtcgc cggtggagta
     1861 gccgggcgcc gcgtcgccgg cgatgcgcat cgccggatag ccgttgaatc ggatcgtctg
     1921 gatcggcccc gtgatccagc gcgtcgtcgc cagcgccgac atcggcaccg gctggccgcg
     1981 cgcattgagc acgttgagct ccagcaggtc ctggggctcc atgcgatacg ccgcgtcggc
     2041 ctggacgatg acacgctgca gcctgccggc gttggggaag tcgttgacgt aggccgagcc
     2101 gagcgccgtc gagagcacgg cgttgatgtt gtcgaacgag acgccgagcg cgttggcgcg
     2161 gtcgcgatcg atgtcgagct gcagttgcgg ggcgtcctcg aggccgtcgg gtcgcacgcc
     2221 ggcgagcacc ttgctcttcg aggcgagtcc gagcagctgg ttgcgcgcgg cgagcagcgc
     2281 cgcgtggccg ttgccgcccc gatcctcgag gcggaaggcg aagccggtgg cggtgcccag
     2341 ctcggggatc gccggcggca cgagcgcgaa gatgaacgcg tcacgcagct tcgaaagggc
     2401 accgaacgcg cgcccggcga tcgccgtcgc cgaatgctcc gtcccttcgc gctcatccca
     2461 cggcttgagg gtgacgaacg ccagtccggc gttctggccg ctgcccgaga agctgaagcc
     2521 gaggacgctg accatctggt cgacctcggg ctgcgacagg aagaacttct cggcctgctc
     2581 catcacggcg agcgttcgcg tcttggtcgc tcccggcggc agctggacgt tggcgatgag
     2641 atagccctgg tcttcggtcg gcaggaacga gcccggcaga cgggtgaaga agagcgcgac
     2701 gagggcgacg acgacgacga acgcgaccag catcgggccg gtgtggcgca ggagcctggc
     2761 gacccagcct tcgtagccgt gcgtcgtcgc cttgaagccg cggttgaacc agccgaacgg
     2821 gccgcgcttc tcgtgcttgg cgcccttctc gagcggcttg aggaaggtgg cgcacaaggc
     2881 cggcgtcagc gacatggcaa ggaagaccga gaacagcatc gagctcacca tcaccagcga
     2941 gaactggcgg tagatgttgc cgacggcgcc cgagaagaag gccatcggca ggaacaccga
     3001 gaccagcgtg acgctgatgc cgaccagcgc cgcggtgatc tgccccatcg ccttcttggt
     3061 cgcgtcgcgc gggctgagcc cttcctcgct catgatgcgc tcgacgttct cgatgacgac
     3121 gatcgcatcg tcgacgagga tgccgacggc cagcaccatc gcgaacatcg tcagcacgtt
     3181 gatcgagaag ccgaaggtct gcatgatccc gaatgcgccg aacagggcca ccgggacgac
     3241 gatggtcggg atcagcgtgt acctgaggct ctgcaggaac aggtacatca ccaggaagac
     3301 gaggatcacc gcctcgacca gcgtctccac gacacggccg atcgagatct tgatgaagcg
     3361 cgagctgtcg tacgggatgc tccactgcac gcccttcggg aagtagcggg ccagctcggc
     3421 catccgtttc ttgatgagct cggcggtctg cagcgcattg ccggtcggcg agagctgcac
     3481 gccgatgccg gtcgagggct tgccgttgag gcgcgccgac gtcccgtagt tctggccgcc
     3541 gagctcgacc cgcgcgacgt cgccgagctt gacggtcgag ccgtcggtgt tggcgcgcag
     3601 cacgatctgg cgaaattcct cgaccgtcgt cagctggccg gcgacgacga cggtcgcggt
     3661 gatcggcgtg ccggggacga tcggcaggtc gccgatggtc cccgaagaca cctgcgcgtt
     3721 ctgcgcccgg atcgcggcga cgacgtcgtt ggtcgacatg ttgtagccga cgagcttggc
     3781 cgggtcgacc cagatccgca tcgcccgttc ggtgccgaag agctgcgcct ggccgacccc
     3841 ggggatgcgc tggatctcgg gcagcacggt gcgcgaggcg tagtcgccga gggcgatcgg
     3901 gtcgagcgag ccgtcgctcg acgacaggat cgtgaacaga aggaagttcg agcgcgcctt
     3961 gtcgacacgc accccctgct gcgtcaccgc cgcgggaagc ctgggcgcgg cgcgcgacag
     4021 gcggttctgc acgtcgacct gggcaaggtc ggggttggtg ccggtctcgt aggtcagggt
     4081 gatctggccg gtgccgttcg actggctcac cgattcgaga tagatgaggc cgggcgaacc
     4141 gttcatctcc tgctcgatga cgctgatgac gctgtcttcg agcgtctgcg ccgaggcgcc
     4201 ggggtaggtc gcgctgagca cgatcgccgg cggcgcgacc gtcgggtact gggcgatcgg
     4261 cagctgcttg atcgcgacgc cgccgaagac gaggatgaaa agcgcgatga cccacgcgaa
     4321 gatgggccgg tcgatgaaga actgcggcat cgcgcgaggt cagcgggaag ccgaggcagc
     4381 gccggccggc gccgctgcgg acgccgctcc gccttccggt ccggacgccg ccccctgcca
     4441 cggcacggcc ttgaccgggg cgtcgccgcg cagcttct
//
LOCUS       DASRWS010000625         7059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1119043, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000625 DASRWS010000000
VERSION     DASRWS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119043"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="mnmH"
                     /locus_tag="VFF72_11900"
     CDS             <1..789
                     /gene="mnmH"
                     /locus_tag="VFF72_11900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HZV93914.1"
                     /translation="LVYCWRGGQRSGALATVLSQIGFRVRLLEGGYRAWRRAVVEALA
                     TLPATFDLRVICGPTGSGKSRLLARLASHGEQVLDLESLANHRGSVLGLVPGSRQPGQ
                     KAFDSRIWEALRALDPARPVFVESESRKIGNLRVPETLVERMRGSPCLWLELPLAARV
                     ALLTRDYDFFVEDTAAFCERLDALRTLRGAALVESWQEAARAGRTAEVVQALLESHYD
                     PIYRHSMQKNFPALAQPAQVISWDGSDASLEVAARSVGAGIDAP"
     gene            complement(923..1654)
                     /gene="phbB"
                     /locus_tag="VFF72_11905"
     CDS             complement(923..1654)
                     /gene="phbB"
                     /locus_tag="VFF72_11905"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HZV93915.1"
                     /translation="MKIAYVTGGMGGIGTSLCQRLHQDGFKVIAGCGPSRDHAKWIAE
                     QAALGYAFDASAGNVGDWNSTESAFAAVKAEHGPIDVLVNNAGITRDVIFRKMTRADW
                     DAVIETNLTSLFNVTKQVIDDMVERGWGRIINISSVNGEKGQFGQTNYSAAKAGMHGF
                     TMALAQELAAKGVTVNTVSPGYIGTDMVKAIRQEVLDKIVAGIPVKRLGTPEDIASMV
                     SWLASDESGFATGADFSVNGGLHMH"
     gene            complement(1746..2933)
                     /locus_tag="VFF72_11910"
     CDS             complement(1746..2933)
                     /locus_tag="VFF72_11910"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZV93916.1"
                     /translation="MSQDNDIVIVAAARTAVGKFGGSLAKTPAPELGAAVIANLLERA
                     KLSGDQVGEVILGQVLAAGSGQNPARQAVVKSGLPQSVPALTINAVCGSGLKAVMLAA
                     QAIRDHDYEIGIAGGQENMSMAPHVLPNSRDGQRMGDWKLVDTMITDGLWDVYNQYHM
                     GITAENVARKYSVSRDKQDALALASQQKAAAAQEAGRFKDEIVPFSIAQKKGDPVVFA
                     ADEFINRKTSAEALAGLRPAFDKAGSVTAGNASGINDGAAAVVVMTAKKARDLGLQPM
                     ARIASYATVALDPAFMGMGPVPASQKALQRAGWKAADLDLLEINEAFAAQACAVNNEM
                     GWDTSKVNVNGGAIAIGHPIGASGCRILVTLLHEMARRGAKKGIASLCIGGGMGVAMC
                     VER"
     gene            complement(2986..4623)
                     /gene="phaC"
                     /locus_tag="VFF72_11915"
     CDS             complement(2986..4623)
                     /gene="phaC"
                     /locus_tag="VFF72_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HZV93917.1"
                     /translation="MAATLGDAWRSLSAVAFPAETMQRLQSDYLADATSIWNRVFSPA
                     VADRAPIGDRRFAAGDWSDNPAAAFAAEMYLLNSRTLLAMADSIEGDEKTRARVRFGV
                     LQWIDAASPANYLALNPEAQRKAIETGGDSIGQGLVHLWNDVQRGHLSQTDESAFEVG
                     RNVATSEGSVVFENELFQLLEYKPLTAKVFERPILFVPPCINKFYILDLQPENSLIRH
                     AVAEGHRVFVLSWKNAKEDIAQRTWDDYIENAAIRAIGLVRDITRSEQINTLGFCIGG
                     TILATALAVCAARGEHPAASLTLLTTLLDFSDTGVLDIFIDEASVRLREMTLGPESLG
                     GGSLLKGQELASTFSFLRPNDLVWNYVVGNYLKGETPPPFDLLYWNSDGTNLPGPMYC
                     WYLRNTYLENKLAKPAALTVCGEELDLGRIAAPSFIYGSREDHIVPWQGAYQSTRVLK
                     GKTRFVLGASGHIAGVINPPLKKKRSHWVGGSAALPASADAWFKTAHERPGSWWTEWS
                     DWLRPFGGRQVAAPGSPGNRAHRPIEPAPGRYVKEKA"
     gene            complement(4920..5642)
                     /gene="pgeF"
                     /locus_tag="VFF72_11920"
     CDS             complement(4920..5642)
                     /gene="pgeF"
                     /locus_tag="VFF72_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZV93918.1"
                     /translation="MTTRVGGVSEGPFASMNLGAGIGDADDVVAANRARLAAACGAVP
                     VFLRQVHGRRVVRLTDADAGAGGVAPTVHEADASVTTSPAVACTVLVADCLPVLFAAP
                     RGRAVGAAHAGWRGLAAGVVEATVEAVCEAGRCRAGELRAWLGACIGPRRFEVGADVL
                     DAFAAGPQAAAGTAFTALRPGKWLADLAALARNRLAAAGVVDVSGDYLCTVEDASRFF
                     SFRRDRVTGRMAAAVWIAGDRR"
     gene            complement(5699..6373)
                     /gene="maiA"
                     /locus_tag="VFF72_11925"
     CDS             complement(5699..6373)
                     /gene="maiA"
                     /locus_tag="VFF72_11925"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HZV93919.1"
                     /translation="MRLYNYFRSSASYRVRIALALKGIAWDYVAVHLVRNEQMGEAFR
                     ALVPAQLVPALVVDGGGDGDGARDGAGSVLTQSLAIIEYLDETHPSPPLLPADPLGRA
                     RVRAIALDMACEIHPLDNLRVLRYLVNDLGVSEDDKNRWYRHWVETGLDVVERRLAAD
                     AATGRFCHGDSPGLADCVLVPQIFNAQRMSCRLDHVPTVMRVFERCMAEPAFASTQPS
                     SCPDAT"
     gene            complement(6385..>7059)
                     /locus_tag="VFF72_11930"
     CDS             complement(6385..>7059)
                     /locus_tag="VFF72_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZV93920.1"
                     /translation="ASLPVAGATGETFPVHRIYCVGRNYVEHAREMGFTGREPPFFFM
                     KPADAIVVVGEGQTGRMTYPSLTKSLHHEVELVVAIGRGGRDIRAADAMAHVWGYAVG
                     LDMTRRDLQGEAKKLGRPWDIGKGFDESAPIGPVRRAAECAIGEATSITLEVNGKMRQ
                     QSTIGKLIWNIAEIIEHLSAAWELAPGDLIFSGTPEGVAAVEVGDTLDASVQGVGSLR
                     LQIAGR"
BASE COUNT         1032 a         2457 c         2439 g         1131 t
ORIGIN      
        1 ctcgtctatt gctggcgcgg aggccagcgc agcggcgcgc tcgccaccgt gctgtcgcag
       61 atcggctttc gcgtgcggtt gctcgaaggc ggctaccggg cctggcgccg cgccgtggtc
      121 gaggcgctcg ccacgttgcc cgcgacgttc gatcttcgcg tcatctgcgg gccgaccggc
      181 tcggggaaaa gccgcctcct cgcccggctc gcgtcgcacg gcgagcaggt gctcgatctg
      241 gaaagcctcg ccaaccatcg cggctcggtt ctcggcctcg tgccgggaag ccggcaaccg
      301 ggccagaagg ccttcgattc gcgcatctgg gaggcgctgc gcgcgctcga cccggcgcga
      361 cccgtcttcg tcgagagcga gagccgcaag ataggcaacc tgcgcgtgcc cgagacgctc
      421 gtcgagcgca tgcgcggctc gccctgcctc tggctcgaac tgccgcttgc ggccagggtc
      481 gcgctgctga cgcgcgacta cgacttcttc gtcgaagaca ccgccgcatt ctgcgagcgg
      541 ctcgacgccc tgcgcacgct tcggggcgcc gcgctcgtcg aatcgtggca ggaagcggcg
      601 cgggccgggc gcaccgccga ggtcgtccag gccttgctcg aatcgcacta cgacccgatc
      661 tatcgacact cgatgcagaa gaatttccct gctctcgcac agccggcgca ggtcatttcg
      721 tgggacggca gcgacgcctc gctcgaggtc gcggcgcggt ccgtcggcgc cggcatcgat
      781 gcgccgtgac accgcgccga cgacgtcgat cagcgcttga ccggccagac gccagcacga
      841 tggcgaccga tgaccgcgca ggtcgtccgg atttgcgtcc ggctttgcgt ccggggacgc
      901 gacccgcgcg acccgtaccg cttcaatgca tgtgcaggcc gccgttgacc gagaagtctg
      961 cgccggtcgc gaagcccgac tcgtccgagg ccagccacga gaccatcgag gcgatgtcct
     1021 cgggcgtgcc gagccgcttc accggaatgc cggcgacgat cttgtcgagc acttcctggc
     1081 ggatcgcctt caccatgtcg gtgccgatgt agcccgggct cacggtgttg accgtcacgc
     1141 ccttggcggc cagttcctgc gccagcgcca tcgtgaagcc atgcatgccg gccttggcgg
     1201 ccgagtagtt ggtctgtccg aactggccct tctcgccgtt gaccgacgag atgttgatga
     1261 tgcgccccca gccgcgctcg accatgtcgt cgatcacctg cttggtgacg ttgaacaggc
     1321 tcgtcaggtt ggtctcgatg acggcgtccc agtcggcccg cgtcatcttg cgaaagatca
     1381 cgtcgcgggt gatgccggcg ttgttgacga gcacgtcgat cggtccgtgc tcggccttca
     1441 ccgcggcgaa ggccgattcg gtcgaattcc agtcgccgac gttgccggcc gaggcgtcga
     1501 aggcatagcc gagcgccgcc tgctcggcga tccacttcgc atggtcgcgg ctcgggccgc
     1561 aaccggcgat gaccttgaag ccgtcctggt ggaggcgctg gcacaacgac gtgccgatgc
     1621 cgcccatgcc gccggtgaca tacgcgatct tcatgcgtgg ctgtccggcc ggatgtcgag
     1681 ccacggcgaa agcgaacggg cagtccctgc gccggacggc cggatccgcg aggcgagcac
     1741 catcgtcatc gctccacgca catcgcgacg cccatcccgc cgccgatgca caggctggcg
     1801 atccccttct tcgcgccgcg cctggccatt tcgtgcaaca gcgtgacgag gatccggcag
     1861 cccgacgcgc cgatcggatg gccgatggcg atcgcgccgc cgttgacgtt gaccttgctg
     1921 gtgtcccagc ccatctcgtt gttcacggcg caggcctgcg ccgcgaaggc ttcgttgatc
     1981 tcgagcagat cgaggtcggc ggccttccag ccggcacgct gcagcgcctt ctgcgacgcc
     2041 ggcaccggcc ccatgcccat gaaggccggg tcgagcgcca cggtcgcgta gctcgcgatg
     2101 cgtgccatcg gctgcaggcc gagatcgcgc gccttcttcg ccgtcatgac gacgaccgcg
     2161 gccgcgccgt cgttgatgcc cgaggcgttg ccggccgtca ccgagccggc cttgtcgaat
     2221 gcgggacgca atcccgccag cgcttcggcg ctggtcttgc gattgatgaa ctcgtcggcg
     2281 gcgaagacga cgggatcgcc cttcttctgg gcgatcgaga acggcacgat ctcgtccttg
     2341 aagcgaccgg cttcctgcgc ggcggcggcc ttctgctgcg acgccagggc gagcgcatcc
     2401 tgcttgtcgc gcgacaccga atatttcctg gcgacgttct ccgccgtgat gcccatgtgg
     2461 tactggttgt agacgtccca caggccgtcc gtgatcatgg tgtcgacgag cttccagtcg
     2521 cccatgcgct ggccgtcgcg cgagttgggc aggacgtgcg gcgccatgct catgttctcc
     2581 tgtccgccgg cgatgccgat ctcgtagtcg tgatcgcgga tcgcctgcgc cgccagcatg
     2641 accgccttca ggcccgaacc gcacacggcg ttgatcgtca gcgccggaac gctttgcggc
     2701 aggccgctct tgacgaccgc ctggcgcgcc gggttctgcc ccgaaccggc ggcgagcacc
     2761 tggccgagga tcacctcgcc gacctggtcg cccgaaagct tcgcgcgttc gagcaggttg
     2821 gcgatgaccg ccgcgccgag ttccggcgcc ggggtcttcg cgaggctgcc gccgaacttg
     2881 ccgacggcgg tgcgggcagc ggcgacgatg acgatgtcgt tgtcttgact catggcgatc
     2941 tcctgtgtgt cggatgtcgt gcagcgccgc ggcgctgcgc tggattcagg ccttttcctt
     3001 gacgtagcgt ccgggtgccg gctcgatcgg ccggtgcgcg cggttgccgg gcgacccggg
     3061 tgcggcgacc tgcctgccgc cgaacggccg cagccagtcg gaccactcgg tccaccagct
     3121 tccgggacgc tcgtgcgccg tcttgaacca ggcgtcggcc gatgccggca gcgcggcgct
     3181 gccgcccacc cagtggctgc gcttcttctt cagcggcggg ttgatgacgc cggcgatgtg
     3241 gcccgaggca ccgaggacga agcgggtctt gcccttcagc acgcgcgtgc tctggtaggc
     3301 gccctgccac ggcacgatgt gatcctcgcg cgagccgtag atgaaggacg gcgcggcgat
     3361 cctgcccagg tcgagctctt cgccgcagac ggtcaacgct gccggcttcg cgagcttgtt
     3421 ctcgagatag gtgttgcgca ggtaccagca gtacatcggc cccggcaggt tggtgccgtc
     3481 gctgttccag tagaggaggt cgaacggcgg cggcgtctcg cccttgaggt agttgccgac
     3541 gacgtagttc cagacgagat cgttcgggcg caggaagctg aatgtcgagg ccagctcctg
     3601 gcccttgagc aggctgccgc cgcccaggct ctcgggcccc agcgtcatct cgcgcagccg
     3661 caccgaggct tcgtcgatga agatgtcgag gacgccggtg tcggagaaat cgaggagcgt
     3721 ggtcaacagc gtgaggctgg ccgccgggtg ctcgccgcgc gcggcgcaga ccgccagcgc
     3781 cgtcgccagg atggtgccgc cgatgcagaa gccgagcgtg ttgatctgct cgctgcgcgt
     3841 gatgtccctg accagcccga tggcgcggat cgccgcgttc tcgatgtagt cgtcccaggt
     3901 gcgctgggcg atgtcttcct tcgcgttctt ccagctgagc acgaagacgc gatggccctc
     3961 ggcgacagca tggcggatca gcgagttctc gggctgcagg tcgaggatgt agaacttgtt
     4021 gatgcagggc ggaacgaaca ggatcggccg ctcgaagacc ttggccgtga gcggcttgta
     4081 ttcgagcagc tggaagagct cgttctcgaa gacgaccgag ccctcgctcg tcgcgacgtt
     4141 gcgaccgacc tcgaaggcgc tttcgtcggt ctgcgagagg tggccgcgtt gcacatcgtt
     4201 ccagagatgg accagcccct ggccgatgct gtcgccgccg gtctcgatcg cctttcgctg
     4261 cgcttccgga ttgagggcga gatagttcgc cggcgacgcc gcatcgatcc actgcagcac
     4321 gccgaagcga acccgggccc gggtcttctc gtcgccctcg atcgaatcgg ccatcgccag
     4381 cagcgtgcgc gaattcagca ggtacatctc ggcagcgaag gcggccgccg ggttgtccga
     4441 ccaatcgcct gccgcgaacc ggcggtcgcc gatcggcgcc cgatcggcca cggccggcga
     4501 gaagacgcgg ttccagatcg aagtcgcgtc ggcgaggtag tcggactgca ggcgctgcat
     4561 cgtctcggca ggaaacgcga cggcgctgag agaccgccac gcatcgccga gcgtcgcggc
     4621 cattttcgcg tcgggggcgc tcggatcggg atgggggcgc gcagtcatgc gcggattatc
     4681 gtgtcgccag tcccgccctg tccgtaggac aagccatgta tctcatcgcc ctcgcctgga
     4741 cctacgtcgt cgtgctgatg gcactcgtcg aaggattctc gaccgagggg acctggctcg
     4801 gtgcgttctt cacgctgctc ctctatggtg cgctgcctct tggcatcacc ctctacctcg
     4861 tcgggacgcc agcacgacgg cgagcgcgcc gcgggggcgc tgagtcggat gagcgcgctt
     4921 cagcgccggt cgccggcgat ccagacggcg gcggccatcc gcccggtgac gcggtcgcgg
     4981 cgaaacgaga agaagcgtga cgcgtcctcg acggtgcaga gatagtcccc gctgacgtcg
     5041 acgacccccg ccgcggccag ccggttacgc gccagcgctg ccaggtcggc cagccatttg
     5101 ccgggtcgca gggccgtgaa ggcagtcccc gcggcggcct gcgggccggc agcgaaggcg
     5161 tcgagcacgt cggcgccgac ctcgaagcgg cgcggcccga tgcaggcgcc cagccaggcg
     5221 cgcagctcgc cggcccggca acggcccgcc tcgcagacgg cctcgaccgt cgcctcgacg
     5281 acgccggcgg ccaggccgcg ccagccggcg tgggcggcgc cgacggcgcg gccacgaggc
     5341 gcggcgaaga gcaccggcag acagtcggcg acgagcaccg tgcaagcgac ggcgggcgac
     5401 gtcgtcacgc tggcgtcggc ctcgtgcacc gtcggcgcga ccccaccggc gccggcatcg
     5461 gcatccgtca ggcgaaccac gcgccggcca tggacctggc gaagaaagac gggaacggcg
     5521 ccgcaggccg cggccagtcg ggcccggttg gccgcgacga cgtcgtcggc gtcgccgatg
     5581 cctgcgccca ggttcatcga tgcgaacggc ccttcgctga cgccgccgac gcgggtcgtc
     5641 atcaaggcgc cgacgccggc ctccggccac cgcggagcga gccactccgc gccgacgctc
     5701 acgtcgcgtc cgggcagctc gacggctgcg tcgacgcgaa cgccggctcg gccatgcatc
     5761 gctcgaagac gcgcatcacc gtcggcacgt gatcgagccg gcacgacatg cgctgcgcgt
     5821 tgaagatctg cggcaccagg acgcaatcgg ccaggcctgg cgaatcgcca tggcagaagc
     5881 gcccggtcgc ggcatcggcg gcgaggcggc gctcgaccac gtcgaggccg gtctcgaccc
     5941 aatggcggta ccagcggttc ttgtcgtctt cggagacgcc caggtcgttg accagatagc
     6001 gaagcacgcg caggttgtcg agaggatgga tctcgcacgc catgtcgagg gcgatggcgc
     6061 gcacgcgcgc cctgccgagc ggatcggccg gcaacagggg cggcgaagga tgggtttcgt
     6121 cgagatactc gatgatggcg agcgactgcg tcagcacgct cccggcgcca tcgcgggcgc
     6181 catcgccatc gccaccgccg tcgacgacga gggcgggcac cagttgcgcc gggaccaggg
     6241 cgcgaaacgc ctcgcccatc tgttcgttcc tgaccagatg gacggcgaca tagtcccagg
     6301 cgatgccctt cagcgccagc gcgatgcgga cgcggtacga cgccgaggag cgaaagtagt
     6361 tgtagaggcg cacgccgctg ccgatcagcg gccggcgatc tgcagccgca gcgagccgac
     6421 gccctgcacc gaggcatcca gcgtatcgcc gacctcgacg gcggcgacgc cctccggcgt
     6481 gccgctgaag atgagatcgc ccggcgccag ctcccacgcg gccgacaggt gctcgatgat
     6541 ctcggcgatg ttccagatca gcttgccgat cgtgctctgc tggcgcatct tgccgttgac
     6601 ctcgagcgtg atcgacgtcg cctcgccgat cgcgcattcg gcggcgcggc gcaccgggcc
     6661 gatcggcgcc gactcgtcga agcccttgcc gatgtcccag gggcgtccca gcttcttcgc
     6721 ctcgccctgc aggtcgcgtc gcgtcatgtc gagccccacc gcgtagcccc agacgtgcgc
     6781 catcgcgtcg gcggcgcgaa tgtcgcggcc gccgcggccg atcgcgacga cgagctcgac
     6841 ctcgtgatgg aggctcttcg tcaggctcgg ataggtcatg cgaccggtct ggccctcgcc
     6901 cacgacgacg atggcgtcgg ccggcttcat gaagaagaac ggcggctcgc ggccggtaaa
     6961 gcccatctcc ctggcgtgct cgacgtagtt gcggccgacg caatagatgc ggtgcaccgg
     7021 gaacgtctcg cccgtcgcac cggcgacggg aaggctggc
//
LOCUS       DASRWS010000626         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_251781, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000626 DASRWS010000000
VERSION     DASRWS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251781"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFF72_11935"
     CDS             <1..609
                     /locus_tag="VFF72_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93921.1"
                     /translation="CEAIACDIGTRDEAARAVAEAVRRFEALDIVVHNAAVNPWTSLA
                     ELNEEALRRTLAVNLEACFWLAQAALPCWQARGSGRMLVTSSVTGPRVAMPRSAPYAA
                     SKAGVNGFIRSAALDLAAEHVTVNGVEPGFIAKIGGTLLSDPERAARIARHIPAGELG
                     RPEDVAYAMCFLASDAGRYITGQTIVVDGGSTLPESPVFAGA"
     gene            614..1393
                     /locus_tag="VFF72_11940"
     CDS             614..1393
                     /locus_tag="VFF72_11940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV93922.1"
                     /translation="MQLRDKVSLVFGASSGIGAAVALRFAREGAHVVAAGLPGPVPGE
                     EAGALGVEARPCDVTVEEQVRAVVREALERHGRIDVLINSAGIIISDEACSIDDRVWA
                     EHCDVNLTGTMRTMRAVLPHMLERGGGAIVNIASVAAFNGGAASASYAASKAGVVALT
                     RSAAQACGARGVRVNALCPGWVDTPMSAREMDELACELGISVDEARERTIARISLARM
                     ARPEEMAAACLFLASDESSFVTGAALVADGGSRVPAAARAV"
     gene            complement(1440..3026)
                     /locus_tag="VFF72_11945"
     CDS             complement(1440..3026)
                     /locus_tag="VFF72_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZV93923.1"
                     /translation="MDWQSPYPERRFPIFARNVVATTQPLAAQAGLAMLRGGGNAVDA
                     ALAAAITLTVVEPIMNGIGGDLYAMVWCDGQLHGLDSTGAAPAGWTPARFAGRTEMPR
                     TGWNSVTVPGQVAGWRALSERFGKLPFARLFAPAIDYAKNGFPVSAFIASVWGRLAGL
                     VDDQPGFADAFMPAGTAPCAGEIWRFPEQAKALADIAATDGNSFYRGDLAQRIIAASS
                     AAGGVLTGEDLASHRAEWVTPLQQRYRDAVLHEIPPNGQGIASLMAAGMLAHFDIRAC
                     GLDSAAALHLQIEAMKLAFADLHAHVGDPRHMRVPASALLDPAYLASRAAAIDPERAG
                     AATAGRPRQAGTVYLAAADSAGMMVSFIQSNYYAFGSGVVVPGTGISMHNRGWNFNLE
                     PGHPNEVGPGKKPLHTIIPAFVTGVDGSPMMAFGVMGGFMQAQGHLQMLSRLADFQQS
                     PQAMNDAPRFMASPLDGSVKVEAHMPAAAVDGLRRRGHRLVVAPTGDLDFGAAQIIQR
                     DGDHYVAASDGRRDGQAVGF"
     gene            complement(3050..3706)
                     /locus_tag="VFF72_11950"
     CDS             complement(3050..3706)
                     /locus_tag="VFF72_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZV93924.1"
                     /translation="MGLLASRTVLDRLQAAILRVAFPDTGSCALRLAELMGEAVVAGK
                     QRASRRRVPVSDDALRTSREIDPAFDLEAFLPFQLSVVANRVSQTVGRAISDRFSLQV
                     PEWRILVVLGKYGSLTALEIARKTSMDKARVSRAQHRLVDLGLAGMQPSADDRRKVIV
                     TLAADGRQLYRDMAPLAHEVADRLFVGLTAGERVALRSVMRKLFASTSDFEPMGDEAV
                     "
     gene            complement(3601..>3877)
                     /locus_tag="VFF72_11955"
     CDS             complement(3601..>3877)
                     /locus_tag="VFF72_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZV93925.1"
                     /translation="DVCRNIGASRRKLNYCFQDVLGSSPARYLRALRLNGVRRALKCP
                     GDRRVGVQDIAARWGFWHLGQFSTDYKRQFCELPSQTLARARCGLPN"
BASE COUNT          631 a         1345 c         1319 g          582 t
ORIGIN      
        1 tgcgaagcga tcgcctgcga catcggcacg cgcgacgagg ccgcgcgcgc cgtcgccgaa
       61 gcggtgcggc gcttcgaggc gctcgacatc gtcgtccaca acgcggctgt gaatccctgg
      121 acctcccttg cagagctgaa cgaggaggcg ctgcgtcgca ccctcgccgt caacctcgaa
      181 gcctgcttct ggctcgcgca ggcggcgctt ccatgctggc aggcgcgcgg ctccggtcga
      241 atgctggtga cgtcttcggt gaccggcccg cgcgtcgcca tgccccgcag cgcgccctac
      301 gcggccagca aggcgggcgt gaacggtttc atccgcagtg cggcactcga cctggccgcc
      361 gagcacgtca ccgtcaacgg cgtcgagccc gggttcatcg ccaagatcgg cggcacgctg
      421 ctgtcggacc ctgaacgcgc cgcacgaatc gcccgccaca tccccgccgg cgagttgggc
      481 cgcccggaag acgttgccta cgcgatgtgt ttcctggcca gcgatgcagg ccgctacatc
      541 accggacaga ccatcgtcgt cgacggcggc tcgacgctgc ccgaaagccc ggtcttcgcc
      601 ggtgcctgac gccatgcagc tgcgcgacaa ggtgtccctc gtcttcggcg cgtccagcgg
      661 catcggtgcc gcagtcgcgc tgcgctttgc acgcgagggt gcgcacgtcg tcgccgcggg
      721 cctgcccggc ccggtgcccg gcgaagaggc gggcgcgctc ggcgtcgagg caaggccctg
      781 cgatgtcact gtcgaagaac aggtccgtgc cgtcgtcagg gaagcgctgg agcggcacgg
      841 ccggatcgac gtcctgatca attcagccgg catcatcatc agcgacgaag cctgctcgat
      901 cgacgaccgg gtctgggccg agcactgcga cgtcaatctc accggcacga tgcggacgat
      961 gcgcgccgtg ctgccgcaca tgctcgagcg cggcggcggc gccatcgtca atatcgcgtc
     1021 ggtggccgcc ttcaatggcg gcgcagcgtc ggcgagctac gccgccagca aggccggcgt
     1081 cgtggcgctc acgcgctcgg cggcacaagc ctgcggcgca cgcggcgtgc gcgtcaacgc
     1141 gctctgcccg ggctgggtcg acacgccgat gtcggcgcgc gagatggacg agctggcgtg
     1201 cgaactcggc atctctgtgg acgaggcccg cgagcgcacg atcgcccgga tttcgttggc
     1261 ccggatggcg cggcccgagg aaatggcggc tgcctgtctc ttcctggcca gcgacgaaag
     1321 ctcgtttgtc accggagcgg cgctggtcgc cgacggtggt tcgcgcgtgc cagccgctgc
     1381 gcgcgcggtc tgattctggc gcgcacccgc tgatgccgcg gcatcgcgcg cacgacgatt
     1441 caaaagccga ccgcctggcc atcgcggcgt ccgtccgagg cggccacgta atggtcaccg
     1501 tcacgctgga tgatctgcgc cgcgccgaag tcgagatcgc ccgtcggcgc gacgaccagg
     1561 cgatgaccgc gccggcgcag accgtcgacg gccgccgccg gcatatgcgc ctcgaccttc
     1621 acgctgccgt cgagcggcga tgccatgaag cgcggcgcgt cgttcatcgc ctgcgggctc
     1681 tgttggaagt cggccagcct cgacagcatc tgcagatggc cctgcgcctg catgaaccca
     1741 cccatcacgc cgaacgccat catcgggctg ccgtcgacgc cggtgacgaa tgccgggatg
     1801 atggtgtgca gcggcttctt gcccgggccg acttcgttcg gatgccccgg ctccaggttg
     1861 aagttccagc cgcggttgtg catcgagatc ccggtgcccg gcacgacaac gccggagccg
     1921 aatgcgtagt agttggactg gatgaaggag accatcatcc ccgccgaatc ggcggcggcg
     1981 agatagaccg tccccgcttg gcgcggccgg ccggccgttg ccgcgccggc tcgctcgggg
     2041 tcgatcgctg cagcccgcga cgcaaggtag gccgggtcga gcagcgccga agccggcacg
     2101 cgcatatggc gcgggtcgcc gacgtgcgca tgcaggtcgg cgaaggcgag cttcatcgcc
     2161 tcgatctgca gatgaagcgc ggcagcgctg tcgagaccac aggcccggat gtcgaaatgg
     2221 gcgagcatgc cggcagccat caacgaggcg atgccctggc cattgggcgg gatctcgtgc
     2281 agcaccgcgt cacggtagcg ctgctgcagc ggcgtgaccc attcggcgcg atggctggcc
     2341 aggtcttcgc cggtcaacac gccgcctgcg gccgaagagg ccgcaataat ccgctgcgcc
     2401 agatcgccgc gatagaacga gttgccatcg gtcgccgcga tgtcggccaa cgccttcgcc
     2461 tgttccggaa agcgccagat ttcgcccgcg cacggcgccg tcccggctgg catgaacgcg
     2521 tcggcgaatc cgggctggtc gtcgacaaga ccggccaggc ggccccatac cgacgcaatg
     2581 aacgccgaca ccggaaagcc gtttttcgca tagtcgatcg ctggtgcgaa aagtcgagca
     2641 aaaggcagct tgccaaagcg ttcggacaag gctcgccatc ccgccacctg gcccggcacg
     2701 gtcaccgagt tccagccggt acgtggcatc tccgttctac cggcgaaacg cgccggggtc
     2761 caacctgcgg gtgcggcacc ggtcgaatcc aggccatgca gttgcccgtc gcaccagacc
     2821 atcgcgtaga ggtcgccgcc gatgccgttc atgatcggct cgacgaccgt cagcgtgatc
     2881 gccgcggcga gcgccgcatc gaccgcattg ccgccgccgc gcagcatcgc cagtccggcc
     2941 tgcgccgcca ggggctgcgt cgtcgccacg acgttgcgcg cgaagatcgg gaagcgacgc
     3001 tcagggtaag gagactgcca gtccacggaa agccttgccg gcgcgcccct cagacggctt
     3061 cgtcgcccat cggctcgaag tcggatgtgc tcgcgaagag cttgcgcatc acggagcgga
     3121 gggccacccg ctcgccggcc gtgagaccga caaacagacg atcggccacc tcgtgggcaa
     3181 gcggcgccat gtctcgatag agttgcctgc catctgcggc cagcgtgacg atgaccttgc
     3241 ggcgatcgtc tgccgagggt tgcatgcccg ccaatccgag gtcgacgagg cgatgctggg
     3301 cccggctgac ccgggccttg tccatgcttg tcttgcgggc gatctcgagc gccgtcagcg
     3361 agccgtactt gccgagcacg accaggatgc gccactccgg cacctggagc gaaaagcggt
     3421 cggagatggc acgaccaacc gtctggctga cgcggttggc gaccacggaa agctgaaaag
     3481 gcaggaaagc ctcgagatcg aacgcggggt caatctcgcg cgacgttcga agcgcatcgt
     3541 ctgagaccgg tacgcgccgc cgcgaggcac gctgttttcc ggccaccact gcctcgccca
     3601 tcagttcggc aagccgcagc gcgcacgagc cagtgtctgg gaaggcaact cgcagaattg
     3661 ccgcttgtag tcggtcgaga actgtccgag atgccagaag ccccaacgcg ccgcgatatc
     3721 ctggacgccg actcttcgat cgcccgggca cttcagggcg cgtcgcacac cattcaggcg
     3781 aagggccctc aggtagcgcg ccgggctgga gccgagcacg tcctgaaagc agtagttgag
     3841 cttgcggcgg ctggcgccga tgttcctgca aacgtcg
//
LOCUS       DASRWS010000627         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1119560, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000627 DASRWS010000000
VERSION     DASRWS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119560"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VFF72_11960"
     CDS             <1..148
                     /locus_tag="VFF72_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZV93926.1"
                     /translation="AIQHLAGMKDSKVIVAINKDAEAPIFSVADYGLEQDLFTAVPEL
                     VRAL"
     gene            236..2062
                     /locus_tag="VFF72_11965"
     CDS             236..2062
                     /locus_tag="VFF72_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZV93927.1"
                     /translation="MSYVAPVKDMLFCMKELADLESIAKLPGFEEAGLDTAQAVLEEC
                     ARFSREVLAPLTREGDVHPSTWKDGVVTTAAGFREAFVQFGQGGWQGLQHPVEFGGQG
                     LPKTIGAACIEMINSANLSFALCPLLTDGAIEALLTAGTAEQQQRFIPKMISGEWTGT
                     MNLTEPQAGSDLALVRTRAEPIGDGRYRLFGTKIFITYGEHDMADNIVHLVLARVAGA
                     PEGVKGISLFIVPKFLVGADGSPGVRNDAWCVSIEHKLGIKASPTAVLQFGDKGVDEG
                     SGVGALPGAIGTLVGEENRGLEYMFIMMNAARYAVGMQGIAIAERAYQKAAAFARERV
                     QSRPVDGSVAGSATIIHHPDVRRMLMTMRAYTEGCRAMAIVAAAAFDAAQRHPDEQTR
                     RQSQAFYEFMVPLVKGFSTEMSIEVASLGVQVHGGMGFIEETGAAQYLRDARILTIYE
                     GTTAIQANDLVGRKTARDGGGVARGVAAQIASTEADLARSASTAALSMKRRLGAAREA
                     FEQVVDFVVRENKARPNAVFAGSVTYLMLAGNLMAGWQMARALLAAERGRDAQAADKS
                     FLEAKIVTARFYAEHLLSKVPALRDAVVDGADSVTALPLEAY"
     gene            2097..3044
                     /locus_tag="VFF72_11970"
     CDS             2097..3044
                     /locus_tag="VFF72_11970"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZV93928.1"
                     /translation="MPLPPVLRSLPLPIIGAPLFIISNPTLVVEQCKAGIVGSMPALN
                     ARPAEQLDEWLAEITETLAAWNREHPERPAAPFAVNQIVHKSNDRLEHDMQVCAKYKV
                     PVVITSLGARTDVNDAVHGWGGVVMHDIINNSFAKKAIEKGADGLIAVAAGAGGHAGV
                     KSPFALVAEIRDWFDGPIALSGAISNGAAVLAARAMGADFAYIGSAFIATDEARAAPA
                     YKQLICESTSDDIVYSNLFTGVHGNYLKGSIRAAGLDPDALPQSDPSKMNFGGDARKA
                     WKDIWGCGQGIGAIKGVVPARELVERLAREYEEARRRIH"
     gene            3311..>3693
                     /gene="sucA"
                     /locus_tag="VFF72_11975"
     CDS             3311..>3693
                     /gene="sucA"
                     /locus_tag="VFF72_11975"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526855.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZV93929.1"
                     /translation="MSEMMQESRANSYLFGGNAPYVEELYEAYLDNPGSVPDNWRSYF
                     DALQNVPATDGSSGRDIAHAPVIESFAQRAKANAFGNKASGDELAVARKQVYVQTLIA
                     AYRSLGSRWADLDPLKRTERTRIPEL"
BASE COUNT          635 a         1235 c         1238 g          585 t
ORIGIN      
        1 cgccatccag cacctggccg ggatgaagga ttcgaaggtg atcgtggcca tcaacaagga
       61 cgccgaggcg ccgatcttca gcgtcgccga ctacggcctc gagcaggatc tcttcaccgc
      121 cgtcccggag ctcgtcaggg cgctttgacg gcgcctgccg atctcgcccg acccggctgc
      181 ccgcctgccc gattcctcgg ccggcgggct ttttctcgac ccggagaatt tcgccatgag
      241 ttacgtcgcg cccgtcaagg acatgctctt ttgcatgaag gagctggccg atctcgaatc
      301 cattgcgaag ctgccgggtt tcgaggaggc gggtctcgac actgcgcagg ccgttctcga
      361 ggaatgcgcc cgcttcagcc gcgaggttct ggcgcccttg acccgcgaag gcgacgtcca
      421 cccctcgaca tggaaggatg gcgtggtcac gaccgcggcc gggttcaggg aggcgttcgt
      481 ccagttcggc cagggcggat ggcaaggcct gcagcatccg gtcgagttcg gcggccaggg
      541 gctgccgaag acgatcggcg cggcctgcat cgagatgatc aacagcgcga acctgagctt
      601 cgcgctgtgc cccctgctca ccgacggggc gatcgaggcg ctgttgacgg ccggcacggc
      661 cgagcagcag caacgcttca tcccgaagat gatttcgggc gagtggaccg gaacgatgaa
      721 cctcaccgaa ccgcaggcag gcagtgacct ggcgctcgtt cgaacgcgtg cggagccgat
      781 cggcgacggc cgctaccgcc tgttcgggac gaagatcttc atcacctatg gcgagcacga
      841 catggcggac aacatcgtcc atctcgtgct ggcccgcgtc gccggcgcac cggaaggcgt
      901 gaagggcatt tcgctgttca tcgtgccgaa gttcctggtc ggcgccgacg gttcgcccgg
      961 cgtgcgcaac gacgcctggt gcgtgtcgat cgagcacaag ctgggcatca aggccagtcc
     1021 gacggcggtc ctgcagttcg gcgacaaggg cgtcgacgag gggtcgggcg tcggtgcctt
     1081 gcccggcgcc atcggcacgc tggtcggcga ggaaaatcgc ggcctcgagt acatgttcat
     1141 catgatgaac gcggcgcgtt acgcggttgg catgcagggc atcgccatcg ccgagcgtgc
     1201 ctaccagaag gcagccgctt tcgccaggga gcgcgtgcag tcgcgaccgg tcgatggctc
     1261 ggtggccggc agcgcgacga tcatccatca ccccgacgtg cggcggatgc tgatgacgat
     1321 gcgcgcgtac accgaaggtt gccgggcgat ggcgatcgtc gcggcggcgg cgttcgatgc
     1381 cgcgcaacgt catcccgacg agcagacgcg ccggcagagc caggcgttct atgaattcat
     1441 ggttccgctc gtcaagggat tcagcaccga gatgagcatc gaggtggcca gcctcggcgt
     1501 ccaggtccat ggcggcatgg gcttcatcga ggaaaccggc gcggcgcagt accttcgcga
     1561 cgcccgcatc ctgacgatct acgaaggcac gacggcgatc caggccaacg atctggtcgg
     1621 ccggaagacg gcgcgcgacg gtggcggcgt cgcccgcggc gttgccgcgc agatcgcgtc
     1681 gacggaagcg gacctggccc gaagcgcaag cacggccgcg ctgtcgatga agcggcgcct
     1741 gggcgccgcg cgcgaagcgt tcgaacaggt cgtcgacttc gtcgtgcgcg agaacaaggc
     1801 gcgtcccaat gcggtcttcg ccggatcggt cacctatctc atgctcgctg gcaacctgat
     1861 ggccggatgg cagatggcgc gtgcgctgct cgccgcagag cgaggccgcg acgcgcaggc
     1921 ggcagacaag tcgtttctcg aagccaagat cgtgacggca cgcttttacg ccgagcatct
     1981 gctcagcaag gttccggccc ttcgcgatgc ggtcgtcgac ggcgccgaca gcgtcaccgc
     2041 gctgcctctc gaagcctatt gacgagttcc ggcgacgccg cgcaacaagg aagcccatgc
     2101 cgctgccccc cgttcttcgc tcgctgcctt tgccgatcat cggcgcgccg cttttcatca
     2161 tcagcaatcc gacgctcgtc gtcgagcagt gcaaggccgg catcgtcggc tcgatgccgg
     2221 cgctcaacgc gcgacccgcc gagcaactcg acgagtggct cgccgagatc acggaaaccc
     2281 tggccgcctg gaaccgggag cacccggagc ggccggccgc gccgttcgcg gtcaaccaga
     2341 tcgtgcacaa gagcaacgac cggctcgagc acgacatgca ggtgtgcgcg aagtacaagg
     2401 taccggtcgt catcacctcg ctcggcgcgc gaaccgacgt caacgacgcc gtgcacgggt
     2461 ggggcggcgt cgtcatgcac gacatcatca acaattcgtt cgcgaaaaag gcgatcgaga
     2521 aaggcgccga cggactcatc gcggtcgccg cgggggcggg cgggcacgcc ggcgtgaaga
     2581 gcccgttcgc gctcgtggcc gaaatccgcg attggttcga tggtcccatc gcgctgtctg
     2641 gcgcgatctc gaacggcgct gcggtcctcg ccgccagggc gatgggcgcc gacttcgcct
     2701 acatcggctc ggcgttcatc gccaccgacg aggcgcgcgc cgcccctgcc tacaagcaac
     2761 tgatctgcga gagcacgagc gacgacatcg tctactccaa tctgttcacc ggcgtgcacg
     2821 gcaactacct gaagggctcg atccgcgccg ccggcctcga tcccgacgct ctgccgcaaa
     2881 gcgacccgag caagatgaac ttcggcggcg acgcccgcaa ggcctggaag gacatctggg
     2941 gctgcggcca ggggatcggg gcgatcaagg gcgtggtgcc ggcacgcgag ctcgtcgagc
     3001 gcctggctcg ggaatacgag gaggcccgcc ggcggatcca ttgagtgcct cggcgctcgt
     3061 cgggcccgac gttcggtcgc aaacctcgct cctcgcgtgg cgcggcctcg ccgagggctc
     3121 cacgattcgc gcgatgcgtc acgacctctt gcacctggcg attcgacccc aagtgttaca
     3181 gtcttcgtga tcgactcttc ccggtatcgc gcgccgcaag gcattcggtc cgggtcgcgt
     3241 tccgtgggcc ggtgaagctg gccggcggga ggttttcaac ccactgcaac tccggaaacc
     3301 ggagtgaaag gtgagcgaga tgatgcagga atcccgggcg aactcgtacc tgttcggcgg
     3361 caacgcgccg tatgtggaag agctgtacga ggcctatctc gacaaccccg gttccgtccc
     3421 cgacaactgg cgcagctatt tcgacgcgct gcagaacgtt ccggccaccg atggatcgag
     3481 cggccgcgac atcgcgcacg cgccggtgat cgaatcgttc gcgcagcgcg ccaaggccaa
     3541 tgccttcggc aacaaggcca gtggcgacga gctcgccgtc gcgcgcaagc aggtctacgt
     3601 ccagacgctg atcgccgcct atcgctcgct cggctcgcgc tgggccgacc tcgatccgct
     3661 caagcggacg gaacgcaccc gcattcccga gct
//
LOCUS       DASRWS010000628         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2266514, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000628 DASRWS010000000
VERSION     DASRWS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266514"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFF72_11980"
     CDS             <1..892
                     /locus_tag="VFF72_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93930.1"
                     /translation="LTDGRYTRVWTPLGENILLADDADGRRLPVEALSRGTREQLFLS
                     LRLALVGSYARRGVELPLVLDDVLVNFDARRAKAAAAVLRDFAAEGHQVLVFTCHEHV
                     WKLFKNLKLPARTLPSHDEAEGATLAYRAPAAETPDEEPEATPLEPAARWRPDNSGLE
                     MMHIENDDVDEEEQEVFAWDTAEDEPEAGEEDEDLSGFEDEPEPPPPRETPPKVTVVR
                     GPTRGGPFDNALWFEPVEDDLDDEDDAEEVDDDPQAGAEDDEEDAFADAGEAEDEDGW
                     DDSDDDDSDDNDSDDDVEAA"
     gene            complement(931..2532)
                     /locus_tag="VFF72_11985"
     CDS             complement(931..2532)
                     /locus_tag="VFF72_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZV93931.1"
                     /translation="MIHVPPLNARRFRLFVIMALIASLDVASNSAVKAAEPATRPNIL
                     YIMTDDHAAHAISAYGSRINQTPHLDRLASEGVRFANCFATNSICTPSRATIITGKYS
                     HLNGVPVFNRFDGSQPTAPKYLQAAGYHTGIIGKWHLGSDPTGFDEWTILPGQGRYFD
                     PVFYDRNKTRTIPGYCTDVITDLAIDFLKRRPAGKPFFLMCHHKAPHRPWEPDEKHKA
                     RFAGKEIPEPPTLYDDYATRTDALRENRQRVFDDMTRLDLKLTPPENLSGKERQQWLQ
                     TKPTEVEIEVDGKRQTLTGDALNKYKYQRYMQDYLACVQAVDDNVGRLLDWLDEQDLA
                     KNTLVIYTSDQGFFLGDHGMYDKRFMYEPSLRMPFIVRWPGVVKPGTVEESIATNCDF
                     APTFLDAAGLDVSADMQGASLVPLLEGKRPADWRKSMYYRYYHDPGHHNTRAHYGVRT
                     ATHKLIYYWRKDQWELFDLVKDPQELNNVYAEPAQQSLVAELKQELYRLKRELKDEDQ
                     FADEQPTTGVDGPPPAKKSPGKASS"
     gene            complement(2643..>3267)
                     /locus_tag="VFF72_11990"
     CDS             complement(2643..>3267)
                     /locus_tag="VFF72_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZV93932.1"
                     /translation="VFVVDDDRRLRDWLVMSLETTGIAARSFASAREFHRFYRPSQPG
                     CLVTDVRLTVQTGLELYEQLLREGKRLPVIFTAASADISTVVAAMKLGAVEFLEKPLD
                     RKTLLEHVKKALTLDAAWRRREAEFNAVAERIACLNARDRETLELILSGASNKVMAAR
                     LFISERAVEMRRARIMRKVQVDSLAELIDLAVTHRVLAEISGVSAAS"
BASE COUNT          582 a          998 c         1083 g          604 t
ORIGIN      
        1 attgaccgac ggtcgctaca cgcgcgtttg gaccccgctc ggcgaaaata ttctcctggc
       61 cgacgacgcc gacgggcggc ggctgccggt cgaggcgctc agccgcggca cgcgcgaaca
      121 attgttcttg agcctgcggc tggccctggt cggctcctac gcgcgccgcg gcgtcgagct
      181 gccgctggtg ctcgacgacg tgctggtgaa tttcgatgcg cgccgggcca aggcggcggc
      241 ggccgtgctg agggacttcg cggccgaagg tcatcaagtg ctggttttca cctgccacga
      301 gcatgtctgg aagctgttca agaatctcaa gctgcccgcc cgcacgctcc cttcccacga
      361 cgaggcggaa ggcgccacgt tggcctatcg ggcgcccgcg gctgaaacgc ccgacgagga
      421 gccggaagcc acgcctctcg aaccggccgc tcgttggcgg ccggacaatt cggggctgga
      481 aatgatgcac atcgagaacg atgatgtcga cgaagaagaa caggaagtat tcgcctggga
      541 cacggctgaa gacgaaccgg aggccggcga ggaggacgaa gatctatccg gcttcgagga
      601 cgaaccggaa ccgccgccgc ctcgcgagac gccgccgaag gtgacggtcg ttcgcggccc
      661 tactcgcggc ggtccgttcg acaatgccct ctggttcgag ccggtcgaag acgatctcga
      721 cgacgaagac gacgcggaag aggtcgacga cgatccccag gccggcgcgg aggacgacga
      781 agaagacgcc ttcgcggacg ccggcgaagc cgaggacgag gacggctggg acgattccga
      841 cgacgacgat tcggacgaca acgattcgga cgacgatgtc gaagcggctt gaagcggcgg
      901 catgcgttag ccgcccgccc gccggctttg tcagctgctc gcctttccgg gcgacttctt
      961 ggctggggga ggtccgtcga cgccggtcgt gggctgctcg tcggcgaatt gatcttcgtc
     1021 tttcaactct cgcttcagcc ggtaaagctc ctgcttcaac tcggccacga gcgattgctg
     1081 cgccggctcg gcgtagacgt tgttcaactc ctgcggatcc ttgaccaggt cgaacagctc
     1141 ccactggtct ttgcgccaat agtaaatcag cttgtgcgtc gccgtgcgca cgccgtagtg
     1201 ggcccgcgta ttgtggtggc cggggtcgtg gtagtagcga tagtacatgc tcttccgcca
     1261 gtcggcgggc cgcttgcctt ctagcaatgg cacaaggctg gcgccttgca tatcggcgga
     1321 aacgtcaagc cccgcggcgt ctaaaaacgt cggggcgaag tcgcagttgg tcgcgatcga
     1381 ttcctcgacc gtgccgggct tcacgacgcc gggccagcga acgatgaacg gcatccgcag
     1441 cgacggctcg tacatgaacc gcttgtcgta catcccgtga tctcccagaa aaaagccctg
     1501 gtcgctggtg tagatcacca gcgtgttctt ggccaggtct tgctcatcga gccaatccag
     1561 cagccgtccg acgttgtcgt cgaccgcctg cacgcaggcc aggtagtctt gcatgtagcg
     1621 ctggtattta tacttattga gcgcgtcgcc cgtgagcgtt tgccgcttgc cgtcgacttc
     1681 gatctcgacc tcggtcggct tggtttgcag ccactgctgc cgctctttgc ccgagagatt
     1741 ctcgggcggc gtcagcttca ggtcgaggcg cgtcatgtcg tcgaacactc gttggcggtt
     1801 ctcgcgcaag gcgtcggtcc gcgtggcata gtcgtcgtac aaggtcggcg gctcggggat
     1861 ttctttgccc gcgaacctcg ccttgtgctt ttcgtcaggc tcccaggggc ggtgcggcgc
     1921 cttgtgatgg cacatcaaga agaacggctt gccggccggc cgccgcttga gaaagtcgat
     1981 cgccagatcg gtgatgacgt cggtgcagta acccggaatc gtgcgcgtct tgttgcggtc
     2041 gtaaaacaca gggtcgaagt agcggccctg gccaggcagg atcgtccact cgtcgaaacc
     2101 ggtcggatcg ctgcccaggt gccacttgcc gattatcccg gtgtggtagc cggcggcttg
     2161 caaatacttg ggcgcggtcg gctgcgagcc gtcgaagcga ttgaacacgg gtacgccatt
     2221 gagatggctg tacttgccgg taatgatcgt ggcccggctc ggcgtgcaga tcgagttggt
     2281 cgcgaagcaa ttggcgaacc gcacgccttc agacgccaag cggtcgagat gcggcgtttg
     2341 gttgatccgg cttccgtagg cgctgatggc gtgcgcggca tggtcgtcgg tcatgatgta
     2401 caagatattc ggccgagtgg ccggctcggc cgcctttacc gccgaattcg acgccacgtc
     2461 gagagacgca atgagcgcca tgatgacgaa caggcgaaat cggcgggcat tgagcggcgg
     2521 gacatgaatc atggcaagct cctcggcgaa gcgatctagc ggggccgcgc ggcggcctcg
     2581 cggctccggc cagtatggac cgcgccgccg cggtggtcaa ttggccgcaa gaattgccgc
     2641 tatcaagagg cggcggagac gccggagatt tcggccagca cgcggtgggt cacggccaga
     2701 tcgatcaatt cggccagcga atcgacttgc accttgcgca tgatccgggc gcggcgcatc
     2761 tcgaccgccc gctcggagat gaacagccgt gcggccatga ctttgttcga ggcgccgctc
     2821 aagatcaact ccaacgtttc gcgatcgcga gcgttcaagc acgcgatgcg ctcggcaacc
     2881 gcgttgaact cggcctcgcg gcgacgccaa gcggcgtcga gcgtcaaggc tttcttgacg
     2941 tgttccagca aggtctttcg atcgaggggt ttttccaaga actcgaccgc ccccagcttc
     3001 atggcggcga cgacggtcga tatgtcggcg ctggcggccg tgaagatgac cggcagccgc
     3061 ttgccttcgc ggagcaactg ctcgtagagt tccaagcccg tctggaccgt caagcgaacg
     3121 tcggtcacca ggcagccggg ttgggaaggc cgatagaagc gatggaattc gcgcgcggag
     3181 gcgaacgatc gtgcggcaat tcccgtcgtt tcgagcgaca tcaccaacca atcgcgcaga
     3241 cggcgatcgt cgtcgaccac gaatacg
//
LOCUS       DASRWS010000629         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1707835, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000629 DASRWS010000000
VERSION     DASRWS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707835"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFF72_11995"
     CDS             complement(<1..254)
                     /locus_tag="VFF72_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93933.1"
                     /translation="METIDILLEPVRAFLYQIGAFLPRLLLAVVVVIGGWLVAKVARF
                     AITKALRAVNFNVLTERAGLDNFLHRGGVASDTTTIFGWLV"
     gene            complement(303..>3566)
                     /gene="treS"
                     /locus_tag="VFF72_12000"
     CDS             complement(303..>3566)
                     /gene="treS"
                     /locus_tag="VFF72_12000"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZV93934.1"
                     /translation="YQLNVKAFFDSDDDGFGDFKGVAAKLDYVRDLGVNTIWLMPFYP
                     SPLKDDGYDISQYDGVHEQYGTLDDFRAMLGEAHRRGLKVITELVINHTSDQHPWFQA
                     ARRAPAGSPERDFYVWSDTDQKYLGTRIIFTDTETSNWTWDPVAKAYFWHRFFSHQPD
                     LNFDNPKVMEAVLDTMRFWLDMGVDGFRLDAIPYLVERDGTTNENLPETHAVIKKLRA
                     AIDAEYKNRFLLAEANMWPEDVREYFGDGDECHMAYHFPLMPRMYMAIAQEDRHPIVE
                     IMQQTPEIPAGCQWAIFLRNHDELTLEMVTSKERDYMYNMYAADKRARINLGIRRRLA
                     PLMENDVDRVKLMNGMLLSMPGSPIIYYGDEIGMGDNVFVGDRNGVRTPMQWSPDRNA
                     GFSRADPQRLYLQPIRDPMYGYEALNVEAQARDASSLLNWTKRMLAVRKTSHAFGRGR
                     RTFIRPGNRKILAYLSEHGEDTILSVFNLSRAAQPVELDLSAYKGRVPVEMLGRTSFP
                     PIGELPYLLTLPSYGFYWFRLSADADVPSWHQEGVALQDRPTLVLFDGWTSFFRDRVM
                     PWRIGMAERMRTQLESDTLPRFIEIQRWYAAKGTPIARARVVDHAIWEADGSSWMLPL
                     VELDGPAEAQSYFLPLAIGWEEGDEQRMNALEPAAVAKVRQQANVGVMGDAFYDESFC
                     RALVVAIRAGLELPMAAGRLRFKATAAFEARDVDVATLPVSRPGAASSNTIVTFDETL
                     FLKGYRRLRRGTNPELDMGRFLTDVVGFAQCVPVLGALEYIGGDGTTMTLALVQGFVA
                     NQGDGWDYTLAYLGRFLEGLRTEGAATGHEAHGGFLALMGTLGQRTAELHLALARRTG
                     DPAFDPEPLAPSDVAQYRQQAADEMAATFALLQQRVAQLPASVRADADAVLARRAALQ
                     ARLAVQPAGDGVDDRAASAGGLKTRYHGDYHLGQILVAGNDFVIIDFEGEPARSFEER
                     RAKGSPLKDVAGMLRSFDYARWSALKQHAHNADEARRLGPPLREWERATREAFLAAYA
                     RTMAAAPAQGGVDAQLLALFELQKAMYELRYELNNRTDWAQVPLQGILALAGSGESR"
BASE COUNT          527 a         1169 c         1285 g          585 t
ORIGIN      
        1 acgagccagc cgaagatcgt cgtcgtgtcg ctggcgacgc cgccgcggtg caggaagttg
       61 tcgaggccgg cgcgctcggt gaggacgttg aagttgacgg cgcgcagcgc cttggtgatg
      121 gcgaatcggg ccaccttggc gaccagccag ccgccgatga cgacgacgac ggcgagcaac
      181 aggcgcggca ggaaggcgcc gatctggtag aggaacgccc gcacgggctc gagaaggatg
      241 tcgatggttt ccatgggcgc tcctcttgcg gatggtggtt cggcgcgccg ccttcgcggc
      301 gatcagcgcg actcgccgct gccggccagc gccaggatgc cttgcagcgg cacctgcgcc
      361 cagtcggtgc ggttgttgag ctcgtagcgc agctcgtaca tcgccttttg cagctcgaag
      421 agggccagca gttgcgcatc gacgccgcct tgcgccggcg ccgcggccat cgttcgcgcg
      481 tacgcggcga ggaacgcctc gcgcgtcgcg cgctcccatt cgcgcaatgg cggcccgagg
      541 cgccgcgctt cgtcggcgtt gtgggcgtgc tgcttcaacg ccgaccagcg cgcgtagtcg
      601 aacgagcgca gcatgccggc gacgtccttc agcggcgagc ccttggcccg ccgttcctcg
      661 aagctgcgcg ccggctcgcc ctcgaaatcg atgatcacga agtcgttgcc ggcgacgagg
      721 atctggccga ggtggtagtc gccgtggtag cgcgtcttca ggccgccggc gctcgccgca
      781 cggtcgtcga cgccgtcgcc cgccggctgg acggccaggc gcgcctgcag cgcggcgcgg
      841 cgcgccagca cggcgtcggc atcggcgcgc accgaggccg gcagctgcgc cacgcgctgc
      901 tgcagcaggg cgaaggtcgc cgccatctcg tcggcggcct gctggcggta ttgcgcgacg
      961 tcgctcggag cgagcggctc gggatcgaac gccgggtcgc cggtgcgccg cgccagcgcc
     1021 aggtgcagct cggcggtgcg ctggccgagc gttcccatca atgccaggaa gccgccatgc
     1081 gcttcatggc cggtggccgc accttcggtg cgcaggccct cgaggaagcg gccgagatag
     1141 gccagcgtgt agtcccagcc atcgccctgg ttggcgacga acccctggac cagcgccagc
     1201 gtcatggtcg ttccgtcgcc accgatgtat tcgagcgcgc ccagcaccgg cacgcattgc
     1261 gcgaacccga cgacatcggt gaggaagcga cccatgtcga gctcggggtt ggtgccgcgg
     1321 cgcaggcgcc ggtagccctt caggaacagc gtctcgtcga aggtgacgat ggtgttgctg
     1381 ctggccgcgc ccgggcgcga gacgggcagc gtcgcgacat cgacgtcgcg cgcctcgaac
     1441 gccgccgtcg ccttgaaccg cagccgcccc gccgccatcg gcagctcgag gccggcgcgg
     1501 atcgcgacca ccagggcgcg gcagaacgac tcgtcgtaga aggcgtcgcc catgacgccg
     1561 acgttggcct gctggcgcac cttggcgacc gccgccggct cgagcgcgtt catgcgctgc
     1621 tcgtcgccct cctcccagcc gatggccagc ggcaggaaat acgactgcgc ctcggccgga
     1681 ccgtcgagct cgacgagcgg aagcatccag ctcgatccgt cggcttccca gatggcgtgg
     1741 tcgacgacgc gggcgcgcgc gatcggcgtg cccttggcgg cgtaccagcg ctggatctcg
     1801 atgaacctcg gcagggtgtc ggactcgagc tgagtgcgca tgcgctcggc catgccgatc
     1861 cgccacggca tgacgcgatc gcggaaaaag ctggtccagc cgtcgaagag gacgagcgtc
     1921 ggccggtcct gcagcgccac gccctcctgg tgccacgacg gcacgtcggc gtcggctgag
     1981 aggcgaaacc agtagaagcc gtacgacggc aacgtgagca gatagggcag ctcgccgatc
     2041 ggcggaaacg acgtgcggcc gagcatctcg accggcactc gtcccttgta ggccgacagg
     2101 tcgagctcga ccggctgggc ggcgcgcgac aggttgaaga cgctgaggat cgtgtcttcg
     2161 ccgtgctcgc tcaggtaggc gaggatcttg cgattgcccg gccggatgaa ggtgcgccgg
     2221 ccgcgcccga aggcgtggct ggtcttcctc accgcgagca tgcgcttggt ccagttgagc
     2281 agcgagctcg cgtcgcgcgc ctgggcctcg acgttgagcg cctcgtagcc gtacatcgga
     2341 tccctgatcg gctgcaggta gaggcgctgc ggatcggccc gcgagaagcc ggcgttgcgg
     2401 tcggggctcc actgcatcgg cgtgcgcacg ccgtttcggt cgccgacgaa gacgttgtcg
     2461 cccatgccga tctcgtcgcc gtagtagatg atcggcgagc ccggcatcga cagcagcatg
     2521 ccgttcatca gcttgacgcg gtcgacgtcg ttttccatca gcggcgccag gcggcggcgg
     2581 atgccgaggt tgatgcgggc gcgcttgtcg gcggcgtaca tgttgtacat gtagtcgcgc
     2641 tccttgctcg tcaccatctc gagcgtcagc tcgtcgtggt tgcgcaggaa gatcgcccat
     2701 tggcagccgg ccgggatctc gggggtctgc tgcatgatct cgacgatcgg atggcggtcc
     2761 tcctgggcga tcgccatgta catgcgcggc atcagcggga agtggtacgc catgtggcac
     2821 tcgtcgccgt cgccgaaata ctcgcgcacg tcctccggcc acatgttggc ctcggcgagg
     2881 aggaagcggt tcttgtactc ggcgtcgatc gcggcgcgca gcttcttgat gacggcgtgc
     2941 gtctcgggca ggttctcgtt ggtggtgccg tcgcgctcga cgagataggg gatagcgtcg
     3001 agccggaagc cgtcgacgcc catgtcgagc cagaagcgca tcgtgtcgag caccgcctcc
     3061 atcaccttcg ggttgtcgaa gttgaggtcg ggctggtggc tgaagaagcg gtgccagaag
     3121 tacgccttgg cgaccgggtc ccaggtccag ttcgaggtct cggtgtcggt gaagatgatg
     3181 cgcgtgccga ggtacttctg gtcggtgtcg ctccagacgt agaagtcgcg ctcgggcgag
     3241 ccggcgggcg cccgcctcgc cgcctggaac cacggatgct ggtcggaggt gtggttgatg
     3301 accagctcgg tgatgacctt caggccgcgc ctgtgcgcct cgccgagcat ggcgcggaag
     3361 tcgtcgagcg tgccgtactg ctcgtgcacc ccgtcgtact gcgagatgtc gtagccgtcg
     3421 tccttcagcg gcgacgggta gaacggcatc agccagatcg tgttgacgcc gaggtcgcgg
     3481 acgtagtcga gcttggcggc cacgcccttg aagtcgccga agccgtcgtc gtcggaatcg
     3541 aagaaggcct tgacgttgag ctggta
//
LOCUS       DASRWS010000630         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1397568, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000630 DASRWS010000000
VERSION     DASRWS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397568"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VFF72_12005"
     CDS             complement(<1..1290)
                     /locus_tag="VFF72_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZV93935.1"
                     /translation="MTLHQIESFALVGAMLALFMSGRLRYDVVAALILLVAILLGVVP
                     SAKAFLGFANPVIIIIASVLVVSRAIAASSILELSVGAVLKRIRSPSLQIGLMSAAVG
                     YLSAFIKNVGTLGIFIPIAIQTARRSQQPRSIYLMPLAFASLVGGTITKIGTSPNLLI
                     VTVQQELGQKQFGLFDFVPVGLPLTTLAIVFLAFGWRLLPHRDGQQTAEESFSIDDYS
                     TELRVVGEKAAAGMTVAGFEAPAEGDISVAAIVREGGRRYLPAEDWRLYEGDVLIVLG
                     DPTAVQKAIAASGFELVGSEELASPAEKSEEMHVVEVIVTAESRLVGETPATLALRHT
                     WGLNLFAVRRAGRPATGRLRSHRFRVGDVVLFQGFEKNLASSVTELGLLPLADRKLSL
                     GQARNGLVPIGLLVAALVLVGLHLLDVEVAFFAAAVLT"
     gene            1460..2671
                     /locus_tag="VFF72_12010"
     CDS             1460..2671
                     /locus_tag="VFF72_12010"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZV93936.1"
                     /translation="MSRVETPPSAALQGLRVVEMGQLIAGPFAGKLLGEFGAEVVKIE
                     APDGGDPLRTWRLLQDGTSVWWQVQSRNKRSIALDLRAAEGQEIARRLVAEADVLIEN
                     FRPGTLEGWGLGPEALLALNPRLVMLRISGYGQTGPYRDRPGFGVVGEAMGGLRHLTG
                     EPGRVPVRCGVSIGDTLAALHGTIGVLLALFHRAAHSGPGQVIDVALHESVFSVMESL
                     IPEYSAFGVVREAAGSALPGIAPSNAYPCRDGYVLVAGNGDSIFRRLMQAIGRADLGN
                     APDLASNAGRVARVVEIDAAIGAWTATRTVGEVLALLADAKVPAGKVYTARDIAEDPH
                     YRARSMLLAQTTRDGHELEVPGIVPKLSATPGSLRSAAPHLGEDTDAVLASLGLGADD
                     IRLLREKGIVA"
     gene            2808..>3498
                     /locus_tag="VFF72_12015"
     CDS             2808..>3498
                     /locus_tag="VFF72_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV93937.1"
                     /translation="MSRSTMLQNLKRGGAPSLRSAVAFLVLAVLAVVTSASTPTAADR
                     KLVVGFVYVTPIGEAGWTYQHELGRRAMQQALGSRVRTIAVESVAEGADAERVMRDLV
                     AQGAGLIFATSFGYLEPALRVAADFPNVRFEHAGGYRTAPNLATYDARYYEARWLAGY
                     LAGATSKAGIAGYVAGFPVPEVVQGINAFTLGMRAANPKAQVRVIWLGTWFDPTRERD
                     AAQSLIDHGADV"
BASE COUNT          527 a         1240 c         1230 g          501 t
ORIGIN      
        1 cgtgagcacc gccgcggcga agaacgccac ctcgacatcg agcaggtgca gcccgaccag
       61 cacgagcgcg gcgacgagca ggccgatcgg caccaggcca ttgcgggcct ggcccagcga
      121 gagcttgcgg tccgccagcg gcagcaggcc gagctcggtg acggaggagg cgaggttctt
      181 ctcgaagccc tggaagagga cgacatcgcc gacgcgaaag cggtgcgatc gcagccgccc
      241 ggtcgccggg cgcccggcgc ggcgaacggc gaacaggttc aggccccagg tgtggcgcag
      301 cgcgagcgtc gccggcgtct cgccgaccag ccgcgactcg gcggtgacga tcacttcgac
      361 gacgtgcatc tcctcgcttt tctccgcggg ggaggcgagc tcctcggagc cgacgagctc
      421 gaagccgctg gcggcgatcg ccttctgcac cgcggtggga tcgccgagga cgatgagcac
      481 gtcaccctcg tagagccgcc agtcctcggc cggcaggtag cggcggccgc cttcgcggac
      541 gatcgcggcc accgagatgt cgccctcggc cggcgcctcg aacccggcca ccgtcatgcc
      601 ggcagcagcc ttttcgccga cgacgcgcag ctcggtcgaa tagtcgtcga tggaaaagga
      661 ttcttccgcc gtctgctggc cgtcgcgatg cggcagcaac cgccagccga aggcgagaaa
      721 gacgatcgcc agcgtcgtca gcggcagtcc gaccggcacg aagtcgaaca ggccgaactg
      781 cttttgcccg agctcctgct gcaccgtgac gatgagcagg ttgggcgacg tgccgatctt
      841 ggtgatcgtg ccgccgacca gcgacgcgaa ggccagtggc atcaggtaga tcgagcgcgg
      901 ctgctggctg cgtcgcgccg tctggatcgc gatcgggatg aagatgccca gcgtgccgac
      961 gttcttgatg aacgccgaga ggtagccgac cgccgccgac atcaggccga tctgcagcga
     1021 cggcgagcgg atccgcttca gcaccgcgcc gaccgagagc tcgaggatgc tcgacgcggc
     1081 gatggcacgg ctgacgacga gcaccgaggc gatgatgatg atcaccggat tggcgaagcc
     1141 gaggaaggcc ttcgccgaag gcacgacgcc gagcaggatc gcgaccagga ggatcagtgc
     1201 ggcgacgacg tcgtagcgca gccgcccgct catgaagagg gcgagcatgg cgccgacgag
     1261 ggcgaacgat tcgatctgat gcagcgtcat gccgggcttt taccgcaggc cgcgaagccg
     1321 cggttccggc gcgatcgcac aatcggcctt cgccgccttc gccgccttcg ccgccttcgc
     1381 tgccttcgcc accgtcgcct ccgtcgccac ccttcgccac ttccgccgga cgcaggaatg
     1441 ccgctgtccg gatttcgcca tgagccgagt cgaaacccca ccgtctgccg cactgcaggg
     1501 cctgcgcgtc gtcgagatgg gccagctcat cgccggacct ttcgcaggaa agctgctcgg
     1561 cgaattcggc gccgaggtcg tgaagatcga agccccggac ggcggcgatc cgctgcgcac
     1621 ctggcggctg ctgcaggacg gcacgtcggt ctggtggcag gtccagtcgc gcaacaagcg
     1681 ctcgatcgcg ctcgatctgc gcgccgccga aggacaggag atcgcgcgcc ggctggtcgc
     1741 cgaggccgat gtgctgatcg agaacttccg gcccggcacg ctcgagggct gggggctcgg
     1801 tccggaggcg ttgctggcgc tcaacccgcg cctcgtgatg ctgcgcattt cgggctacgg
     1861 ccagaccggg ccctatcgcg accggccggg cttcggcgtc gtcggcgagg cgatgggcgg
     1921 cctgcgccat ctgaccggcg agccgggccg cgtgcccgtt cgctgcggcg tctccatcgg
     1981 cgacacgctc gccgccctgc acggcacgat cggcgtgctg ctggcgctct ttcaccgcgc
     2041 cgcccacagt ggcccggggc aggtcatcga cgtggcgctg cacgagtcgg tcttcagcgt
     2101 catggaaagc ctcattcccg aatacagcgc gttcggcgtc gtcagggagg ccgcgggcag
     2161 cgccttgccg ggcatcgcgc cgtcgaacgc ctacccgtgt cgcgacggct acgtgcttgt
     2221 cgccggcaac ggcgacagca tcttcaggcg gctcatgcag gcgatcggcc gcgccgacct
     2281 gggcaacgcg cccgatctcg ccagcaacgc cggccgcgtc gcccgcgtcg tcgagatcga
     2341 tgcggccatc ggcgcgtgga ccgcgacccg caccgtgggc gaagtcctgg cgctgctcgc
     2401 cgacgcgaag gtgccggccg gcaaggtcta caccgcccgc gacatcgccg aggatccgca
     2461 ctaccgggcc aggtcgatgc ttctcgcgca gacgacccgc gatggacacg agctcgaggt
     2521 gcctggcatc gttcccaagc tgtcggcgac gccgggctcg ctgcgcagcg cggcgccgca
     2581 tctgggcgag gacaccgacg ccgtcctcgc ttcgctcggc ctcggcgcgg acgacatccg
     2641 gctgcttcgc gaaaaaggca tcgtcgcctg aagcgcggcg ctccggcgcc cgccgtaggt
     2701 cgtgccctcg cgccggcgcg aggcggccgg gcttcgtcgg cgagaatgac gctcgccggt
     2761 cgcgtgcccc cgcggccggc ggcgagccgt tcgattccat gcgggccatg tcgaggtcaa
     2821 ccatgttgca aaacctcaag cgcggcggcg cgccttcgct gcgatccgcc gtcgcgttcc
     2881 tcgtcctcgc cgtgctggcc gtcgtgacgt cggcatcgac tcccacggcg gcggaccgaa
     2941 agctcgtcgt cggcttcgtc tacgtgacac cgatcggcga ggccggctgg acgtaccagc
     3001 acgagctcgg tcgtcgcgcc atgcagcagg cgctcggctc gcgcgtgcgg acgatcgccg
     3061 tcgagtcggt tgccgaaggt gccgacgcgg aacgcgtgat gcgcgatctg gtcgcgcagg
     3121 gcgcggggct gatcttcgcg acgagcttcg gctatctcga gccggcattg cgcgtcgccg
     3181 ccgacttccc gaacgtccgg ttcgagcacg ccggcggcta ccgcacggcg cccaacctcg
     3241 cgacctacga cgctcgctac tacgaggcgc gatggctggc cggctacctg gccggggcga
     3301 cgagcaaggc cggcatcgcc ggctacgtcg ccggcttccc ggtgcccgag gtggtgcagg
     3361 gcatcaacgc cttcacgctc ggcatgcggg cggcgaaccc caaggcgcag gttcgcgtca
     3421 tctggctcgg cacctggttc gacccgacgc gggagcgcga cgcggcgcag tcgctgatcg
     3481 accacggcgc cgacgtgc
//
LOCUS       DASRWS010000631         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2269254, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000631 DASRWS010000000
VERSION     DASRWS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269254"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFF72_12020"
     CDS             complement(<1..503)
                     /locus_tag="VFF72_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV93938.1"
                     /translation="MTAAIPFVVRAAQPRDLQAIVRLIAGLAEFEKLTHLLAVTPESL
                     RPHLFGERPAAEALVVDKGDAQVAFALFFSNFSTFLGKPGLYLEDLFVEPAHRGQGIG
                     RALLMRLAQLARERGCGRFEWSVLDWNENAIRFYEQMGATVMPEWRICRVTGDALASF
                     ATPGMPDP"
     gene            673..1617
                     /locus_tag="VFF72_12025"
     CDS             673..1617
                     /locus_tag="VFF72_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV93939.1"
                     /translation="MTPDPYLPRRHARSRFVEARGLRHHVLEWGDATTTSEDRPTLVM
                     LHGWMDVAASFQFVVDAFAADRHVVALDWRGFGQSETPQADTYFFAEYLGDLETVLDA
                     LFGADRGPLDLLGHSMGGNVAMLYAGVRPALVRRLVNLEGFGMPRTAPSRAPARLAAW
                     LDALKTPEQLRPYDSLEAVAARLQKTNPLLRGDRAAWLAAQWSERLVDQGGGESRRLL
                     ADPNHKRASPLLANVDEWLECWKRISAPVLWVEGDRTDVSIWWGDRYTKAEFHERLNL
                     VADAERHVVSPAGHMLHHDRPEDIARLIETFLSESRPS"
     gene            1643..2026
                     /locus_tag="VFF72_12030"
                     /pseudo
     CDS             1643..2026
                     /locus_tag="VFF72_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552084.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
BASE COUNT          334 a          740 c          668 g          284 t
ORIGIN      
        1 ggatcgggca ttccgggcgt ggcgaacgac gccagcgcgt cgccggtgac gcggcagatg
       61 cgccactcgg gcatcaccgt cgcacccatc tgctcgtaga agcggatggc gttctcgttc
      121 cagtcgagca cgctccattc gaaccgtccg cagccacgct cccgcgccag ttgcgccagc
      181 cgcatgagga gggcgcggcc gatgccctgg ccgcgatgcg cgggttcgac gaacaggtcc
      241 tcaagataga ggccgggctt gccgaggaag gtcgagaagt tgctgaagaa cagcgcgaag
      301 gcgacctgcg catcgccctt gtcgacgacc agcgcctcgg cggcgggccg ctcgccgaac
      361 agatgcggtc gcaggctctc gggcgtgacc gcgagcaggt gcgtcagctt ctcgaactcg
      421 gcgaggccgg cgatcaaccg gacgatggcc tgcagatcgc gcggctgcgc cgcgcggacg
      481 acgaacggaa tcgcggcagt catgtcgccg attctgcccc gctccccggg gcgtccgacg
      541 accggctcgg cgccgcgccc gcccgctcgc gcgccggggc agccgacgcg aacggcgacg
      601 aactgtcgca tcgccgacag gcgtgcggtc gccgcccgct tacattggcc gcacccgaac
      661 cgaccgcctg tcatgacacc ggatccctat cttccccgcc gccacgcgcg cagccgcttc
      721 gtcgaggcgc gcggccttcg ccatcacgtt ctcgaatggg gcgacgcgac gacgacgagc
      781 gaagaccggc cgacgctggt gatgctgcac ggctggatgg acgtcgccgc gtcgttccag
      841 ttcgtcgtcg acgccttcgc cgccgatcgc cacgtcgtcg ccctcgactg gcgcggcttc
      901 ggccagagcg aaacgccgca ggccgacacc tatttcttcg ccgagtacct gggcgacctc
      961 gagaccgtgc tcgacgccct cttcggcgcc gaccgtggcc cgctcgacct cctcggccac
     1021 agcatgggcg gaaacgtcgc catgctctac gccggcgtgc ggccggcgct cgttcggcga
     1081 ctcgtcaacc tcgagggctt cggcatgccg cgcacggcgc cgtcgcgcgc gcccgcgcgc
     1141 ctggccgcct ggctcgacgc gctgaagacg cccgagcaac tgcgccccta cgactcgctc
     1201 gaggcggttg cggcgcgcct gcagaagacc aacccgttgc tgcgcggcga tcgcgcggcc
     1261 tggctggccg cgcagtggtc ggagcgcctc gtcgaccaag gcggcggcga gtcgagacga
     1321 ctgctcgccg acccgaacca caagcgcgcc agcccgcttc tcgccaacgt cgatgaatgg
     1381 ctcgagtgct ggaagcggat ctcggcgccg gtcctctggg tcgagggcga ccgcaccgat
     1441 gtgtcgatct ggtggggcga ccgctacacc aaggccgagt tccacgagcg gctgaacctc
     1501 gtcgccgacg ccgaaaggca cgtcgtttcg ccggccggcc acatgctcca ccacgaccgg
     1561 cccgaggaca tcgcccgact gatcgagacg ttcctgtcag aatcgcggcc ttcctgaagc
     1621 gttaccgccg cgagaccgca ccatggacgt cgaacacatc aacgccatcg gcaatcgact
     1681 tgccgacctg acccagcgta cccaggctct tcgggggtat ctttgactac gatcgcaaag
     1741 cgctgcgcct gaacgaggtc aacgcggcgc tcgagaaccc gaacgtctgg aacgagccca
     1801 agcgggcgca ggaactgggc cgcgagaagc gcgccctcga caccgtggtg agcacggtcg
     1861 atcacctcac cagcaacctg gccgacaaca ccgagctcta cgacatgtcg aagtccgacc
     1921 aggacttcga cggcctgctg gcgatcgagg ccgacgcggc caagctcgag gcgacggtcg
     1981 cggggctcga gttcaggcgc atgttcaacc agcccgagga tccgct
//
LOCUS       DASRWS010000632         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2270478, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000632 DASRWS010000000
VERSION     DASRWS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270478"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /gene="dnaG"
                     /locus_tag="VFF72_12035"
     CDS             <1..1391
                     /gene="dnaG"
                     /locus_tag="VFF72_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZV93940.1"
                     /translation="KFYRAQLKESPAAIAYLKRRGLTGVVAAHFGIGYAASGWQNLAQ
                     AFTAYDDPTLDATGLVIAGDNGKRYDRFRDRIMFPIHDSRGHVIGFGGRILDSGEPKY
                     LNSPETAVFSKGRELYGLYLARNAIRDAGRVVVVEGYMDVVALAQHGVGYAVATLGTS
                     TTAVHAQKLMRLSDSVVFCFDGDAAGRKAAWRALENVLPALVDGKEARFLFLPDGEDP
                     DDFVRKRGKEAFERALETATPLSEYLLAELAARHSPTTAEGRAALVAAARPYVGQIEA
                     PILATLVRRQLAELADLPEAQLGSLVASLPAAAHGGRSTSSSSRRRAPPAPARRPPSL
                     LRALLTGLVLEPALARRHDLPRVDGAGAEAAAWNAVLDHCLHEAGDPSTASIVQHFAD
                     TAHGELIAQVLAAAADQAIGADQVEVDVLAAVEKLRQAEDQRVVTALLTQPLSTLTAA
                     QREMLAKAGRKA"
     gene            1555..>2657
                     /locus_tag="VFF72_12040"
     CDS             1555..>2657
                     /locus_tag="VFF72_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HZV93941.1"
                     /translation="MAKKHVKPARKSRPATRSKSAARKPAAKSGSRKPATAASRRSPA
                     KRPAAKPSGTRRATGRTTASKPKAAPKSKPSAKAAKPAPAPTTKPDKLDRGTTRFDRG
                     TTRLDRGTTRLDRGTKLVAATPVAAAKSVPNRKPSVGEIAAKIAGKRAEDAAHRKGKN
                     GKHAEVAPRELTPADVEARKRRLKTLIVLGKERGYLTYAEINDHLPDDILDAEQIEGI
                     ISMIGDMGIQVYDEAPDAETLLMSESTPSVPAEDVEEEAEAAASTLDSEFGRTTDPVR
                     MYMREMGSVELLTREGEIEIAKRIEEGLKHMIQAISACPMTVAQILDLADKIGKDEMK
                     NDDVVDGLMDFDEGIQPVAAAAESSDDEEEEED"
BASE COUNT          466 a          898 c          955 g          338 t
ORIGIN      
        1 cgaagttcta tcgcgcacaa ctgaaggaat cgccggcggc gatcgcttac cttaagcggc
       61 gtggcctgac cggcgtcgtc gccgcgcatt tcggcatcgg ctacgcggcc tccggctggc
      121 agaacctggc gcaggcgttt accgcctacg acgatccgac gctcgacgcg accggcctcg
      181 tcatcgccgg cgacaacggc aagcgctacg accgcttccg cgaccggatc atgttcccga
      241 tccacgacag ccgcgggcac gtgatcggct tcggcggccg cattctcgac agcggcgagc
      301 ccaagtacct gaactcgccc gagacggcgg tcttctccaa gggtcgcgag ctctacggcc
      361 tgtacctcgc acgcaatgcg attcgcgatg cgggccgcgt cgtcgtcgtc gaaggctaca
      421 tggacgtcgt cgcgctcgcg cagcacggcg tcggctacgc ggtggcgacg ctcggcacgt
      481 cgacaactgc cgtgcacgcg cagaagctga tgcggctttc cgacagcgtc gtcttctgct
      541 tcgacggcga tgccgccgga cgcaaggccg cgtggcgcgc gctcgagaac gtgctgccgg
      601 cgctcgtcga cggcaaggag gcgcggttcc tgttcctgcc cgacggcgag gatcccgacg
      661 acttcgtacg caagcgcggc aaggaagcgt tcgaacgggc gctcgaaacc gcgacgccgc
      721 tttcggaata cctgcttgcc gagctcgcgg cacggcattc gccgacgacc gccgagggac
      781 gggccgcgct cgtggcggcc gccaggccgt acgtcgggca gatcgaggcc ccgatcctcg
      841 cgaccctcgt gcgccgccaa ttggccgaac tcgccgacct tccggaggcc caactcggat
      901 cgctggtggc gtcgctgccg gcggcggctc acgggggccg cagtacgtcg tcttcgtccc
      961 gccggcgggc accgcccgcc ccggcgcggc ggccgccgtc gctgttgcgc gcgctcctga
     1021 ccgggctcgt gctggagccg gcgctcgcgc gaaggcacga cttgccgcgc gtggacgggg
     1081 ccggcgccga ggcggctgcc tggaacgcgg tcctcgacca ttgcctgcac gaagcgggcg
     1141 acccgtcgac ggcgagcatc gtccagcact tcgccgacac ggcacacgga gagttgattg
     1201 cacaggtgct cgcggcggcg gccgatcagg cgatcggcgc cgaccaggtg gaagtcgacg
     1261 tcctggctgc ggtggaaaag ctgcggcagg cggaggacca gcgggtcgtg acggccctgc
     1321 tgacgcagcc gttgtcgacg ctgaccgccg cccagcggga gatgctggca aaggcggggc
     1381 gcaaggcttg acggtcgacg gatggccgtg cgttttgcgc ttcgcgcgct ataatttgag
     1441 gtttttcgcg ccgcccccat tgtctttcgg cggtgcgcaa aaccggaatg cggagacggc
     1501 gcaccggcgg tgcgccctga cccggggcgg cgccgttacc gaacaaggat gatcatggcc
     1561 aagaagcacg ttaagcccgc ccgcaagtcg cgtcctgcga ccaggagcaa gtcggcggcg
     1621 aggaaaccgg cggcgaaaag cggaagcagg aagccggcga ccgccgcgag ccggcgctcg
     1681 cctgcgaagc gtccggcggc aaagccgtcc ggcacgaggc gcgcgacggg caggacgacg
     1741 gcgtcgaagc cgaaggcggc gccgaaatcg aagccctccg ccaaggcggc gaaaccggcg
     1801 ccggcgccga ccaccaagcc tgacaagctc gaccggggga cgacgaggtt cgaccggggg
     1861 acgacgaggc tcgaccgggg gacgacgagg ctcgaccggg ggacgaagct cgtcgccgcg
     1921 acgccggtcg cggcggcgaa gtcggtcccg aaccgcaagc cgtcggtcgg cgagatcgcg
     1981 gcgaagatcg ccggcaagcg cgccgaggac gcggcgcacc ggaagggcaa gaacggcaag
     2041 cacgccgagg tcgcgccgcg cgagctgacg ccggccgacg tcgaggcgcg caagcgccgc
     2101 ctcaagacgc tgatcgtgct cggcaaggag cgcggctacc tcacgtacgc cgagatcaac
     2161 gaccaccttc ccgacgacat tctcgacgcc gagcagatcg agggcatcat ctcgatgatc
     2221 ggcgacatgg ggatccaggt ctacgacgag gcgcccgatg ccgagacgct gctgatgtcg
     2281 gaatcgacgc cgtcggtccc ggccgaggac gtcgaggagg aagccgaagc cgccgcgtcg
     2341 acgctcgaca gcgagttcgg ccgcacgacc gacccggtgc gcatgtacat gcgcgagatg
     2401 ggatcggtcg agcttctgac gcgcgaaggc gagatcgaga tcgcgaagcg gatcgaggaa
     2461 ggcctgaagc acatgatcca ggcgatctcc gcgtgcccga tgacggtcgc gcagatcctc
     2521 gacctcgccg acaagatcgg caaggacgag atgaagaacg acgacgtcgt cgacggcctg
     2581 atggacttcg acgagggcat ccagcccgtc gcggcggccg ccgagtcgag cgacgacgag
     2641 gaagaggaag aggacga
//
LOCUS       DASRWS010000633         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3450961, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000633 DASRWS010000000
VERSION     DASRWS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3450961"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFF72_12045"
     CDS             complement(<1..802)
                     /locus_tag="VFF72_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93942.1"
                     /translation="MNSAVAPSVTARLAGIGTAVLGLVAMAGWALDKAALRSVLRGAV
                     EMKANTAASLILAGTALYLGAHGSSSHQRATRLLAIVVAMVGVATGLEYALGLNLHID
                     ELLFVDTGKAYNAIRGRMSPYSTVGFLGIGMALALLPSMRWRPVVWLLSALVAGIGLV
                     SMLGYVWNASELVTDSVLPPVAIHTGLAFTLLGIGTLRASRPRLAMAAGTLPNLASIE
                     LKVAAGLFGAFILLIAAGGIAYETSAAAAASARAVLRSQEIRSTLHQLY"
     gene            complement(924..2276)
                     /locus_tag="VFF72_12050"
     CDS             complement(924..2276)
                     /locus_tag="VFF72_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV93943.1"
                     /translation="MMGEIEQRTHALETSNLEKVREVEERRVAQQQVMSLNEELERRV
                     LERTAELERSNGELVVATAAAHEANRAKSEFLSNMSHELRTPLNAIIGFGQLLAAGEA
                     RLSTPERNKEFVNHIVEAGRHLLTLINDILNLAQIEAGKLSVSVEEVILAEVLDECRA
                     MAETMANQRNIRLLFPPRDAITIRADRTRIKQVLLNLLSNAVKYNRDMGSVVVGCERV
                     RPQAIRISVQDTGIGMRAEQLKSLFQPFNRLGQEAGVQEGTGIGLVLTKRLVELMGGS
                     LSVTSTPGVGTVFSIELEATEAESQAARAEPVETGTDRSASESATMTVLCVEDNPVSL
                     KLIEEVVGAREGIRLLTAANGRDGLAVARARLPDVILMDNNMPVMSGREAQRLLSADP
                     RTANIPVIAISANAMTGAVAEGLAAGYFRYLTKPVDLGDLRRALDDALALAMQRRPPA
                     "
     gene            2247..>2556
                     /locus_tag="VFF72_12055"
     CDS             2247..>2556
                     /locus_tag="VFF72_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93944.1"
                     /translation="MSALLDLAHHLVEGGDQCADLVVAVALDAARVVALVDDLARRRS
                     DRQDRAGDDALQAVRHEQRDQHRQQRDRSGNPGVCPDAADEVVLRTQVDGAQDLPVAD
                     D"
BASE COUNT          439 a          836 c          848 g          433 t
ORIGIN      
        1 catagagctg gtggagcgtg cttcgaatct cctggctgcg cagaacggcc cgagccgacg
       61 cggccgcagc cgcgcttgtc tcgtaggcga ttccgccggc ggcgatgagc aggatgaacg
      121 caccgaaaag acccgctgcg accttgagct cgatggaagc gaggttgggc agggtgcccg
      181 cggccatcgc caggcgcggg cggctcgccc gcaaggttcc gatgccaagc agcgtgaagg
      241 ccagcccggt atgaatcgcg accggcggga gcacgctgtc ggtgacgagc tcgctggcat
      301 tccagacata gcccagcatg ctcacgagac cgattcctgc caccagagcc gaaagaagcc
      361 agacgacggg tcgccaccgc atgctcggca gcagggccag cgccatgccg ataccgagga
      421 aacccacggt gctgtacggc gacatgcgac cgcgaatggc gttgtatgcc ttgccggtgt
      481 cgacgaagag caactcgtcg atgtgaagat tcaatccaag cgcgtactcc aggccggtcg
      541 cgacaccaac catcgcaacg acgatcgcga gaagccgggt cgctcgttga tgcgatgaag
      601 acccatgggc accaaggtac agggcggttc cggccaggat cagacttgcc gctgtgttgg
      661 ccttcatttc aacggcgccg cgcagcacac tgcgcaaggc ggccttatcg agcgcccatc
      721 ccgccatggc gaccaggccc agcaccgccg tgccgatgcc tgccagccgg gcggtgaccg
      781 aaggtgcgac cgcggaattc atgggaagtc gaaaacgcat cgaatgaggc aacccggggc
      841 ttcggtcact gagttcggcc atgcaggaaa gaaatcgtgc ggcatcctcg atccagcggg
      901 atgaggtcgc gtgccgatgt cgctcaggcc ggcggccgtc gttgcatggc caatgccagt
      961 gcgtcgtcga gcgccctgcg cagatcgccc aggtcgaccg gcttggtcag atagcgaaag
     1021 tagccggcgg caagcccttc ggccacggcg ccggtcatgg cgttggcact gatcgcgatg
     1081 accggaatgt tcgccgtgcg tgggtcggcg gacagcaacc gctgcgcctc gcgaccgctc
     1141 atcaccggca tgttgttgtc catcaggatg acatcgggaa ggcgagcccg cgccacggcc
     1201 agtccgtcgc gcccgtttgc cgctgtcagc agacgaatcc cctcgcgcgc accgaccact
     1261 tcttcgatca gcttcaggct gacggggttg tcctcgacgc atagcacggt catcgtggcg
     1321 ctctcggagg cggacctgtc ggtgccggtc tcgacgggct cggcgcgagc agcctgcgac
     1381 tcggcttcgg tcgcctccag ctcgatggaa aacacggtcc ccacccctgg ggtgctggtc
     1441 accgacaggc tgccgcccat gagctcgacg aggcgcttgg tcagcacgag accgattccg
     1501 gtcccttcct gcacgcccgc ttcctggccg agtcggttga aaggctggaa cagcgacttg
     1561 agttgctcgg cgcgcattcc gatcccggtg tcctggacgg agatccggat cgcttgcggg
     1621 cgcacgcgct cgcagccgac gacgacggag cccatgtcgc ggttgtactt gacggcattc
     1681 gacagcaggt tgaggaggac ctgcttgatt cgcgtccggt cggcgcgaat cgtgattgcg
     1741 tctcgcggcg ggaagaggag gcggatgttg cgctggttgg ccatcgtctc ggccatggca
     1801 cggcactcgt cgagcacctc cgcgagaatg acttcctcga ccgataccga gagctttcca
     1861 gcttcgatct gcgccagatt caggatgtcg ttgatcagcg tgagcaggtg cctgcctgcc
     1921 tcgacgatgt ggttcacgaa ctccttgttc cgctcgggcg tcgacagccg cgcttcgccg
     1981 gccgccagca actggccgaa tccgatgatc gcgttgagtg gcgtgcgcag ctcgtggctc
     2041 atgttggaga ggaactcgga cttggcacgg ttggcctcat gcgcggcggc cgtcgccacc
     2101 accagctcgc cgttggaccg ctcgagctcg gccgtacgct cgaggacgcg gcgctccagc
     2161 tcttcgttga ggctcatcac ctgttgctgg gcgacgcggc gctcctccac ctcgcgcacc
     2221 ttttcgagat tggacgtctc gagtgcgtga gtgcgctgct cgatctcgcc catcatctcg
     2281 ttgaaggcgg cgaccaatgc gccgacctcg tcgtcgccgt ggccctcgac gcggcgcgag
     2341 tagtcgcgct cgtcgacgac ctcgcgcgcc gccgaagcga tcgccaggat cgggcgggtg
     2401 atgatgcgct gcaggcggta cgacacgagc agcgcgacca gcatcgccag cagcgcgacc
     2461 gcagcggcaa tcccggtgta tgcccagatg cggccgacga ggtcgtactc cgcacgcaag
     2521 tagacggtgc ccaggatctg cccgttgccg acgatg
//
LOCUS       DASRWS010000634         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2538952, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000634 DASRWS010000000
VERSION     DASRWS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538952"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1351)
                     /locus_tag="VFF72_12060"
     CDS             complement(140..1351)
                     /locus_tag="VFF72_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZV93945.1"
                     /translation="MNARILACRFAALASLLVAWMLLGAGCAPSGPQVFEKAAADATD
                     DLVAQTGKLPAFLAKIEMTLSRPDANAPKRRVVLDPMLDMITGQQDETTQLLERRVTQ
                     RMGLKYPQFEFLPFQAANLTRAQYLMTGTIARVAGSSPRQVRVSLSLVDLKSTLVVAQ
                     ASALALEENLDNTPSPYYKDSPVLIKDKVVEGYARTATTPVGQHADPYYMARVGASSV
                     ISEATTLYNAERYQDALGQYQTALATPEGKQLRTESGIYLTNMKLGHVSEAEQAFGRI
                     VALGIAYNELGVKFLFSPGSTEFWSDPKISGAYPMWLRQIAREAVAAGSCMKIVGHTS
                     KTGPAAVNDAISLKRAAYIRQRLAAEEPVLAGRMQVEGMGFRQNIVGSGTDNAFDVLD
                     RRVEFKIQPCS"
     gene            complement(1348..>2996)
                     /locus_tag="VFF72_12065"
     CDS             complement(1348..>2996)
                     /locus_tag="VFF72_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZV93946.1"
                     /translation="AIDDETEHARPAPPLGHMLAAGSRLRDYEITGLIGEGGFGIVYL
                     AWDHSLQRKVAIKEYMPASMASRVSGSSAIVVKSERHLDTFKAGLRSFVNEARLLARF
                     DHPSLVKVYRFWEENGTAYMVMPFYEGPTLKSALASLGHVPTEDELRAWLKPVLNAVS
                     VLHDGETWHQNIGPDSILLTPVGPVLLGFASAAHAIAASSHLPAAALKPGYAPIEQYG
                     GVAETTRGPWTDLYSLAAVVYGAITGEEPIAAADRLADDRLAPLSTIAAGLYSPRFLA
                     AVDAALAVQPELRPQDHLHFRALMGDIETPTRLELAPPRDLMHEPFAGRDDRPVEVTV
                     PDPPPAPAAVATPAAPAAPTKPAPRRPSPPAAVAAAAAAAAESGPSWMRLRQRTNSFG
                     KRALYGLIAVTCALIGLVALVFSYQDRGERRTASATPPAASSPSTVSNAGPAVAVAAS
                     PVVAKAVASIAPSAPVSPVSPPPTLVADRLPNATAATTAARAAAPVTAAAATLAATPG
                     RPPDATTAADRQTRCLDILQKASLEPISQTETDYFKRECK"
BASE COUNT          440 a          949 c         1131 g          476 t
ORIGIN      
        1 cggcgagcgc cgatccgggc agatcgggcg gcctcgcttc gtcgtcgtgg ttcgtggcca
       61 tcagcgatcg gacatcaggt cggcctcgac agcggcgccg tcgggccgcg cgagcccgcg
      121 cacctggccg gccggccgct caactgcaag gctggatctt gaactcgacg cgtcgatcga
      181 gcacgtcgaa ggcattgtcg gtgccgctgc cgacgatgtt ctgccgaaag cccatgccct
      241 cgacctgcat gcgaccggcc aggaccggtt cctcggcggc caggcgttgc cggatgtagg
      301 cggctcgctt gagagagatc gcatcgttga cggccgccgg cccggtcttg ctggtgtgac
      361 cgacgatctt catgcagctg ccggcggcga cggcttcgcg cgcgatctgc cgcagccaca
      421 tcgggtaggc gccactgatc ttcgggtcgg accagaactc ggtgctgccg gggctgaaca
      481 ggaacttgac gccgagctcg ttgtaggcga tgccgagggc gacgatcctg ccgaaagcct
      541 gctccgcctc ggagacgtgg ccgagcttca tgttcgtgag gtagatgccg ctttccgtgc
      601 gcagctgctt tccctccggc gtcgccagcg ccgtctgata ctggccgagc gcatcctggt
      661 agcgctcggc gttgtagagc gtcgtcgcct cgctgatcac gctcgaagcg ccgacccgcg
      721 ccatgtagta cggatcggcg tgctggccga ccggcgtcgt cgccgtcctg gcgtagcctt
      781 cgacgacctt gtccttgatg aggacggggc tgtccttgta gtagggcgac ggcgtgttgt
      841 cgaggttttc ctcgagcgcc agggccgagg cctgggcgac gaccagcgtc gacttcaggt
      901 cgaccagcga caggctgacg cgaacctggc gcggactcga gcctgcgacg cgcgcgatgg
      961 ttccggtcat caggtattgc gcccgggtca gattggccgc ctggaagggc aggaactcga
     1021 actgggggta cttgagaccc atccgctgcg tgacgcgccg ctcgagaagc tgggtcgtct
     1081 cgtcctgctg gcccgtgatc atgtcgagca tcgggtcgag gacgacgcgt cgcttcggcg
     1141 cgttcgcgtc gggtcgggag agcgtcatct cgatcttcgc caggaacgcg ggcagcttgc
     1201 cggtctgggc gacgagatcg tcggtcgcgt cggcggccgc cttctcgaag acctgcggac
     1261 ccgaaggcgc gcagccggcg ccgagcagca tccaggcgac gagcagcgag gcgagcgcgg
     1321 cgaagcggca ggccaggatg cgggcgttca tttgcattcc ctcttgaaat agtcggtttc
     1381 ggtctgactg atcggctcga gagaggcctt ttgcaggatg tcgaggcaac gcgtctgacg
     1441 gtctgccgcg gtcgtcgcgt cgggtggcct tcctggcgta gccgcaaggg ttgcggcggc
     1501 cgcggtgacg ggcgccgccg cgcgggcagc ggtcgtcgcg gccgtggcgt tcggcaagcg
     1561 gtcggcgacg agcgtcggcg gcggtgacac cggcgacacc ggcgccgagg gcgcgatcga
     1621 ggccacggcc ttcgcgacga ccggcgacgc cgcgacagcg accgccggac cggcgttcga
     1681 caccgtggag ggcgacgacg cagcgggtgg tgtcgcagac gcagtgcgcc gctcgccacg
     1741 gtcctgatag ctgaagacga gtgcgaccag gccgatcagc gcgcaggtca cggcgatgag
     1801 cccgtacagc gcgcgcttgc cgaacgagtt ggtgcgctgg cgaaggcgca tccaggaagg
     1861 gccggattcg gccgcggcgg ccgccgccgc cgctacggct gccggcggcg aaggacgccg
     1921 cggcgccggc ttggtcggcg ccgcaggggc cgccggcgtc gccaccgccg caggtgcggg
     1981 cggtggatcg ggcacggtga cttcgaccgg tcgatcgtcc ctgccggcga acggttcgtg
     2041 catgagatcg cggggcggcg ccagctcgag ccgggtcggc gtctcgatgt cgcccatcag
     2101 ggcacggaaa tgcaggtgat cctgcggacg cagttccggc tggacggcca gggccgcgtc
     2161 gacggcggcg aggaagcgcg ggctgtagag ccctgcggcg atcgtcgaca gcggcgcgag
     2221 acggtcgtcg gcgagacggt cggctgcggc gatcggctct tcgcccgtga tcgcaccata
     2281 gaccaccgcg gccagcgaat acaggtcggt ccacggcccc cgcgtcgtct cggcgacgcc
     2341 gccgtactgc tcgatcggcg cgtagcccgg cttgagtgcg gcggcgggaa ggtggctcga
     2401 cgcggcgatc gcatgcgccg ccgaagcgaa tccgagcagg accggaccga ccggagtgag
     2461 aagaatcgaa tcggggccga tgttctggtg ccacgtctcg ccgtcgtgca gcacgctgac
     2521 ggcgttgagg acgggcttga gccaggcacg caactcatcc tcggtgggga cgtgtccgag
     2581 cgaggcgagg gcggacttga gggtgggccc ttcgtagaag ggcatgacca tgtaggcggt
     2641 gccgttctct tcccagaagc gatagacctt gacgagggag ggatggtcga agcgggcgag
     2701 caggcgggcc tcgttgacga agctcctgag gccggccttg aaggtgtcga ggtgtcgctc
     2761 ggacttgacg acgatggccg aggagcctga gacgcgagag gccatggagg cgggcatgta
     2821 ctccttgatg gcgacctttc gctgcaggga gtgatcccag gcgagataga cgatgccgaa
     2881 gccgccctcg ccgatgagtc cggtgatctc gtagtcgcgc agccggctgc ccgccgccag
     2941 catgtggccg agcgggggcg ccggacgggc gtgttccgtc tcgtcgtcga tcgcct
//
LOCUS       DASRWS010000635         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3124162, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000635 DASRWS010000000
VERSION     DASRWS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3124162"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..318
                     /locus_tag="VFF72_12070"
     CDS             163..318
                     /locus_tag="VFF72_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93947.1"
                     /translation="MSEGGMLYLVMTVLAFLMFCGTLMWAMIETSPRRGPKRAGGGVR
                     RPILHHH"
     gene            378..1043
                     /gene="hisG"
                     /locus_tag="VFF72_12075"
     CDS             378..1043
                     /gene="hisG"
                     /locus_tag="VFF72_12075"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZV93948.1"
                     /translation="MPTANETLVLALPKGRILDEVMPVLARARIAIEPSFADPQSRQL
                     RFRTSEPSLDVIRVRNFDVATFVAFGAAQLGVAGNDVLMEFDYPEIYVPLDLRVGRCR
                     LVVAAPQEDVGHDDPRRWSHVRVATTYPEITRRHFAARGVQAECVKLQGAIELAPSIG
                     LCRRIVDLVDSGRTLRDNGLVEIEHIGDVTSRFIVNRAALKTQPERVGHWVDRFREVS
                     RAA"
     gene            1033..2349
                     /gene="hisD"
                     /locus_tag="VFF72_12080"
     CDS             1033..2349
                     /gene="hisD"
                     /locus_tag="VFF72_12080"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZV93949.1"
                     /translation="MPPEGPTRLDASSPGFEKDFSAFLSRNRDTDENVDRIVADIVAD
                     VRARGDAALVEYTRKFDRVETDAAGLRVSEAERRAAAAQASAAQREALEFAARRIEAF
                     HRALLPKDVDFTDHTGTRLGARYRPLDSVGVYVPGGTAAYPSSVLMNIVPAKVAGVKR
                     VVMVVPTPGGTLNPLVMLAAEIAGADEIWRIGGAQAVAALAYGTASMRPVDKITGPGN
                     AFVAAAKRRVFGQVGIDLIAGPSEILVVADRHNDPAWIAADLLSQAEHDASSQSVLIA
                     DDKAFAGAVIRAVDTELKTLARADIAAASWRDNGAIILVPDLAQSAPIVDRIAAEHVE
                     LAMEPEAAEALSQKIAHAGAIFLGRHTPEAIGDYVAGTNHVLPTSRAARFSGGLSVAD
                     FLKRTSIVGCSPAALAELGPAALALAEAEGLDAHGRSISIRLNRRG"
     gene            2409..>2788
                     /locus_tag="VFF72_12085"
     CDS             2409..>2788
                     /locus_tag="VFF72_12085"
                     /inference="COORDINATES: protein motif:HMM:NF002769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HZV93950.1"
                     /translation="MLDEKSVARRSPEVDHKRAVALFDLLEENDFALVNGEPGPYKLH
                     IGIFEQRLVFSVHAADDRKLRDVILSLTPFRKIVKDYFLICGSYYAAIKKLGPAQIEA
                     LDMGRRGLHNEGSELLRERLDGKI"
BASE COUNT          428 a          958 c          925 g          477 t
ORIGIN      
        1 tttccgcctt cgcgacgctc gccctcacac tcgcgctgct cgccatcgcc ttccgcctct
       61 acgagcgcga gcaaatcctc tttccgcatt gacgcgggag tggctttctc gcgaccggcc
      121 gccgcccttg cgtttaatga gcaggaacgc gggaggtggc ccatgtccga aggcggaatg
      181 ctctatctcg tcatgaccgt cctggccttt ctgatgtttt gcggcacgct gatgtgggcg
      241 atgatcgaga cgagcccgcg ccggggcccg aagcgggcgg gcggcggcgt ccggcgcccg
      301 atccttcatc atcattaacg gttgccggca cgcgcgcgca tttgcctcgc ccggcgccgg
      361 gaggctaggc tggccggatg ccgacggcca acgaaaccct ggttctggcg ctgcccaagg
      421 ggcgcattct cgacgaggtg atgcccgttc tggcgcgcgc gcgcatcgcg atcgagccgt
      481 ccttcgccga tccccaatcg cgccagctcc gcttcaggac gagcgagccg tcgctcgacg
      541 tgatccgcgt caggaacttc gacgtcgcga ccttcgtcgc ctttggcgcg gcgcagctgg
      601 gcgtcgccgg caacgacgtg ctgatggagt tcgactatcc cgagatctac gtgccgctcg
      661 atctgcgcgt cggccgctgc cgcctcgtcg tcgcggcgcc gcaggaggat gtggggcatg
      721 acgacccgcg gcgctggagc catgtgcgcg tcgcgacgac ctatcccgag atcacccgcc
      781 gccacttcgc cgcgcgcggc gtgcaggccg aatgcgtcaa gctccagggc gcgatcgagt
      841 tggcgccgag catcgggctc tgccgccgca tcgtcgatct ggtcgattcg ggccgcacgc
      901 tgcgcgacaa cggcctggtc gagatcgagc acatcggcga cgtcacctca cgcttcatcg
      961 tcaatcgcgc cgcactcaag acgcagccgg agcgcgtcgg ccactgggtc gatcgctttc
     1021 gcgaggtgag ccgtgccgcc tgaggggccg acaagactcg acgcctcgtc gcccggtttc
     1081 gaaaaggact tctcggcttt cctctcgcgc aaccgggaca ccgacgagaa cgtcgaccgt
     1141 atcgtcgcag acatcgtggc cgacgtgcgc gcgcgcggtg acgcggcgct ggtcgagtac
     1201 acgcggaaat tcgacagggt cgagaccgac gccgcgggcc tgcgtgtctc cgaagccgag
     1261 cgccgtgccg ccgcggcgca ggcatcggcg gcgcaacgcg aggcgctgga attcgcggcc
     1321 cggcgcattg aagccttcca tcgcgcgctc ttgcccaagg acgtcgactt caccgatcac
     1381 accggtacgc ggctcggggc gcgctatcgg ccgctcgact cggtgggtgt ctacgtgccg
     1441 ggtggcacgg cggcctatcc ctcgtcggtg ttgatgaaca tcgtgccggc caaagtggcg
     1501 ggcgtgaaac gagtcgtcat ggtcgtgccg acgccgggtg gcacgctcaa tccattggtc
     1561 atgctggctg ccgagattgc cggcgctgac gagatctggc gcatcggcgg cgcacaggcc
     1621 gtggcggcgc tcgcttatgg cacggcgtcg atgaggccgg tcgacaagat caccggcccg
     1681 ggcaatgcct tcgttgccgc cgccaagcgg cgcgtgttcg gccaggtcgg tatcgacttg
     1741 atcgcaggtc cctcggagat cctagtggtc gccgatcggc acaacgatcc cgcctggatc
     1801 gccgccgatc tcctgtccca ggccgagcac gatgcctcct cgcagtcggt gttgattgcc
     1861 gacgacaagg ccttcgctgg ggcggtcatc cgggcggtcg atacagagct gaagaccctc
     1921 gcgcgcgccg atatcgcggc ggccagctgg cgtgacaacg gcgccatcat cctggtgccc
     1981 gacctagcgc aatcggcgcc gatcgtcgat cgcatcgccg ccgagcatgt cgagctcgcg
     2041 atggaaccgg aggccgccga ggcgctgagc caaaagatcg cccatgccgg ggcgattttc
     2101 cttggccgtc atacgcccga ggccatcggc gactatgtgg ccggcaccaa tcacgtgctg
     2161 ccgacctcgc gggccgcgcg tttctcgggc ggcctttccg tcgcggattt cctgaagcgc
     2221 acctcgatcg tcggctgctc tccggcagcg ctggcggaac tcggtccggc ggcattggcg
     2281 ctcgccgagg cggaagggct tgacgcgcac ggccgttcca tctccatccg gttgaaccgc
     2341 cgcggctgac gaccatggca catccgccat ccccggcagg gaccaggcca cggcgcatcg
     2401 tcgacatcgt gctcgacgag aaatcggtgg cgcggcgctc gcccgaggtc gaccacaagc
     2461 gcgccgtcgc gttgttcgat ctcctagagg agaacgactt tgccctcgtg aatggcgagc
     2521 ccggccccta caagctgcat atcggcatct tcgagcagcg gctggtgttc agcgtccatg
     2581 ccgccgacga ccgcaagctg cgcgacgtca tcctgtcgct gacgccgttc cgcaagatcg
     2641 tgaaggacta ctttctaatc tgcggcagct actacgcagc tatcaagaag ctgggaccgg
     2701 cgcagatcga ggcgctcgac atgggccgcc gcggcttgca caacgagggt tccgaactgc
     2761 tgcgtgagcg gctcgacggc aagatcga
//
LOCUS       DASRWS010000636        11123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_255676, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000636 DASRWS010000000
VERSION     DASRWS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255676"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFF72_12090"
     CDS             complement(<1..434)
                     /locus_tag="VFF72_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV93951.1"
                     /translation="MNRFSRRACVRVAGWATLAALTALAGCSKKEETTTSTEATPAGS
                     TTTTTTTTTTGTMSASAPATAASGPLKVAFAYVGPVGDGGWTFAQDNARKALQAALGD
                     KIQTSFVEKVPEGADAERVFRDMASQGNKLIFGTTFGYMEPML"
     gene            complement(626..1006)
                     /locus_tag="VFF72_12095"
     CDS             complement(626..1006)
                     /locus_tag="VFF72_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HZV93952.1"
                     /translation="MVDTRTMRWQDWVNLILGAWLFISPWVIGFYSQMPGPSWNFFVI
                     GVAMAVFAIVGLNVYSLWEEWVNLVLGIWMVISPWIVGYSGTVSARDDSIIVGVIVGV
                     LAIWALADRNRLLGDGTATRTLSH"
     gene            complement(1177..2124)
                     /locus_tag="VFF72_12100"
     CDS             complement(1177..2124)
                     /locus_tag="VFF72_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93953.1"
                     /translation="MDQAALLIAATLNSGTVLAFAALGLLVNERAGIVNLGAEGTMLV
                     AAVAGFAVAVHTGSDWAGFGAGALAGALLASAFGVLVIWLNTNQYATGLALSLFGTGF
                     SAFVGQPFTQQRLAERASFHVPGLSDLPFFGPALFRQHPMVYLAIAMTAALAWFLYRS
                     RAGLVLRAIGESPESAHALGYPVRRIRLLAVAFGGALCGVAGAYVSVVYTHLWVEGMI
                     AGKGWIALALTTFATWRPARVLLGAYLFGGVTMLQFQLQQSDGFHVPGIAGAVQVPSQ
                     FLTMLPYLATIVVLVLISRNATFIRVNMPASMGKPFFPG"
     gene            complement(2124..3314)
                     /locus_tag="VFF72_12105"
     CDS             complement(2124..3314)
                     /locus_tag="VFF72_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93954.1"
                     /translation="MLRLEARPEPSRAMSMASPLIALAITVVIGTLLFVVLGKDPLRG
                     LGVFFVEPLKGAYALSELAIKATPLLLIALGLAVCFRSNIWNIGAEGQFVLGAIFASG
                     VAMQATKDTGHWIFIAVLAASVVGGMLWAAIVAFLRDRFNASEILVSLMLVYVAEQLL
                     NYFVYGPWKDPHGYNFPQTVTFLKSTQVPRLFDTYRINIGAVGALVAVGAFWVLLFRT
                     YAGFQLQVGGLAPAAARYAGFSARRALWTTMLVSGGMAGLAGGLEVAGPLNQLTPHVP
                     AGYGFAAIIVAFVGRLHPVGIVFSAILMSMFYIGGELSQSRLGLPQSLTGVFQGLLLL
                     ALLACDTLIAYRVRWRTGVAGAAATSSSASPPAASAPATSELDDAPLSPVVATSALPA
                     RKGS"
     gene            complement(3301..4836)
                     /locus_tag="VFF72_12110"
     CDS             complement(3301..4836)
                     /locus_tag="VFF72_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV93955.1"
                     /translation="MLRLELVDISKQYPSVKANDRVALRVEPGEIHAVLGENGAGKST
                     LMKIIYGAVRPDEGEIRWNGAPVQIATPAAARALGISMVYQHFSLFDTLTVAENVWLG
                     LDRSLPLAEVTQRIRKVAGVYGLEIDPLRPVHTLSVGECQRVEIVRALMSEPKLLILD
                     EPTSVLTPQAVQALFVTLRKLAAEGCAILYISHKLDEIRALCHRCTVLRGGRVTGVVD
                     PTGQSNASLSRLMIGAEPPQLQHREAHAGEVVLEVRELSLTRDDPFGTSLDRISFDLR
                     AGEVLGIAGVSGNGQQELMRALSGEDVRAPRGSIRLFGNDIARDRPRRRRRQGLHFIP
                     EERLGRGAVPTLSLAQNTLLTRTGAVAASGWVHTGAVTELAATLIRRFNVKAGGPGSA
                     ARSLSGGNLQKFIVGREIDADPKLLVVSQPTWGVDVGAAAQIRGELLALRDRGSAVLV
                     VSEELDELFEICDRLVVIAQGRVSPSVAVADASVERIGEWMSGLWHGAAPAGSMELGH
                     AAA"
     gene            complement(4850..6154)
                     /gene="guaD"
                     /locus_tag="VFF72_12115"
     CDS             complement(4850..6154)
                     /gene="guaD"
                     /locus_tag="VFF72_12115"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HZV93956.1"
                     /translation="MTAGARIAVRGDLLDFTAEPAWGDVEPAALRFRPDHWLLVEDGR
                     ITGVQRGDPGEGWQRHDHAGRLVLPGFIDTHVHSAQIDVIASYGTELLDWLTTHTFPA
                     EARHADAAHADAAAAHFLDALLAHGTTSAVVFPTVHRRSVDALFAAAESRGMRIVAGK
                     VLMDRNAPDDLRDDVDSAERDMAELIARWHGRGRLAYAVTVRFAPTSSPEQLTLAGRL
                     CAGDASLYLQTHLAENRAELRWVAELFPDARSYLDVYASAGLLHRRSIFAHGVWLDDT
                     DRAMLAAAGAQIAHSPSSNLFLGSGLFDWRAAHAAGVNVSLASDVGGGTSLAMPRIMA
                     EAYKVQALAGQRLTAWQALHAATRGAARSLDLGGEIGSLETGTIADLCVWDWAAGPVA
                     ERRLEVARSLHERVFAWMTLADERNLAAAFVAGRRHYDRGSH"
     gene            complement(6151..7110)
                     /locus_tag="VFF72_12120"
     CDS             complement(6151..7110)
                     /locus_tag="VFF72_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZV93957.1"
                     /translation="MPSESAFGKIELQLVRVLHTVISERSVSRAAMRLGSSQPAVSAQ
                     LRRLRGLTGDALLVRAGNRMTPTDTALRLLGPAAALLDEAERLFGARARQRGFDPAAS
                     EATLRIAASDYLDPSFLPGIVGHVKRQAPSIRLELLPLSAEYDYRQRLAAGEVDLVVG
                     NWLRPPGELHLGRLFSDEVVCLVAADHPATRRGWSADRYLAAEHVAPMPFHANAPGVV
                     DDHLASLGLARDVAVKSAHFSLIPLMVAQSRLVLTTGRLFCSRFVDSLPLKIVRCPIA
                     FPPLTYYQLWHEVTHASASTKWLREQIRAVARDLAVPAPARPS"
     gene            7197..8891
                     /gene="xdhA"
                     /locus_tag="VFF72_12125"
     CDS             7197..8891
                     /gene="xdhA"
                     /locus_tag="VFF72_12125"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HZV93958.1"
                     /translation="MSSRPIRFFHRGAVVEIDAAATTRTVLDWLREDAHCTGTKEGCN
                     EGDCGACTVVIGEIAGREAKSAPDVVDGLRLRSANACLQFLPTLDGKALFTVEDLATA
                     VVAAPHRHADGLRHPPQAPDRAPGASGEAGAVAAKAGAAEVGAACAASASLHPVQRAM
                     VDCHASQCGFCTPGFVMSLWAMYQHHLASATRPSRQQIADELSGNLCRCTGYRPIVDA
                     GERMFDAPPARLDARAVVAALEGLNTTAAPDFVSPGGDIFLAPHSLDEFARQRLARPE
                     ARVLAGATDIGLWVNKMFRPLPELLYIGHVAELKRIEERDHVLSIGAAASLEDAWRSL
                     VARWPSLAEVWLRFAGVPLRHTGTMGGNVANGSPIGDSAPVLMALGASLLLRQGDRLR
                     RLPLSEFYTGYMTNRLEAGEFLQAIEVPLDTRASVRAWKISKRFDCDISAVCAGLAIE
                     LDEAGSVVAARFAFGGMAATAMRAAGAERAVLGRAWNEATVVAAQTALDADFAPLTDV
                     RGGAGYRRLVARNLLRRLWLETRRETPLASTQTRVWQVLPPVATPARADTFIGAPP"
     gene            8888..>11123
                     /gene="xdhB"
                     /locus_tag="VFF72_12130"
     CDS             8888..>11123
                     /gene="xdhB"
                     /locus_tag="VFF72_12130"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HZV93959.1"
                     /translation="MNRALDASWLAPTDSGDAQALGAGARVGISRPHESAHLHVAGDA
                     PYVDDLPELAGTLHAALGLSPVAHGRIVALDLAGIAALPGVVAVLTARDIPGPNDCGA
                     VVHDDPILADGIVHYVGQPVFAVIATDRLAARRAAAEAKSALRIEPLPALLTPVEAHA
                     AASYVLPPMHLVRGDARAAIDSAPHRFTGSLEVGGQEQFYLEGQISYAVPREDRGMLV
                     HCSTQHPSEMQHHVASCLGLSSHHVQVECRRMGGGFGGKESQSALFACVAAVAAQRLQ
                     VPVKLRLDRDDDFLVTGRRHCFHYEYEVGHDDEGRILGAELTMVSRAGFSADLSGPVM
                     TRAVCHFDNAYWLPDVAIHGYSARTNTQSNTAFRGFGGPQGAIAVENILDTVARRLGK
                     DPLDVRRLNFYGPSASAAGPGERNVTPYGQTVEDNILHELVGELEATSRYRERRAQVD
                     AFNAGSEVLKRGLALTPVKFGISFNLVHLNQAGALVHVYGDGSVLVNHGGTEMGQGLN
                     TKVAQVVAHELGVDFDAVRVTATDTHKVANTSATAASTGSDLNGKAAQDAARQIKARL
                     VAFAAERYGVEASAVRFANGSVSIGTTRTLGFRELVVEAYMARVQLWSDGFYATPGLS
                     WDAATMRGRPFFYFAYGAAVSEVVVDTLTGEWKLLAAHVLHDAGRSLNPAIDLGQVEG
                     GFIQGMGWLTTEELVWHPDDGTSRAGLLLTHAPSTYKIPTANDCPPVFDVCLWKDGLG
                     AGDNV"
BASE COUNT         1788 a         3947 c         3858 g         1530 t
ORIGIN      
        1 agcatcggct ccatgtagcc gaaggtggtg ccgaagatga gcttgttgcc ctggctggcc
       61 atgtcgcgaa agacccgctc ggcatcggcg ccctcgggca ccttctcgac gaacgaggtc
      121 tggatcttgt cgccgagtgc ggcctgcagc gccttcctgg cgttgtcctg ggcgaacgtc
      181 cagccgccgt cgccgaccgg gccgacatag gcaaaggcca ccttcagcgg cccgctcgcc
      241 gccgtcgccg gcgccgaggc cgacatcgtt ccggtcgtcg tcgtggtggt cgtggtcgtc
      301 gtcgatccgg ccggcgtcgc ctcggtgctg gtcgtcgttt cttccttctt gctgcaaccg
      361 gcgagggccg tcaaagcggc cagcgtggcc cagccggcga cccgcacaca agcccgtctt
      421 gaaaatcgat tcatgggtga ctccacgtgg aaaaccgttg ttcgaggcga agccgcgcga
      481 cgccgagaga cgattatcaa tgaagccaac agggatacga atgtcctgcg ccgccggaac
      541 cggcgcgcac cgtcccgacg cttcggaaac ggacgatccg cgaagcgaga tgcggcgcgc
      601 gaaaggtgcg cgcgccgcct tctcgtcagt gactgagcgt gcgggtggcc gtgccgtcgc
      661 cgaggagacg attgcgatcg gccaacgccc agatcgccag gacgccgacg atgacgccga
      721 cgatgatcga atcgtcgcgg gccgaaaccg tgccgctgta gccgacgatc cagggcgaga
      781 tcaccatcca gatcccgagc acgaggttga cccattcctc ccagagcgag tacacgttca
      841 ggccgacgat ggcaaagacg gccatggcca cgccgatcac gaagaagttc cagctcggac
      901 cgggcatctg cgaatagaac ccgatgaccc agggggaaat gaacagccag gcacccagga
      961 tgaggttgac ccaatcctgc catcgcatgg ttctcgtatc gaccatgatt gactccttgc
     1021 gctgcgtcgt tgaccgatct cgacgaatgc cgcgcagcgc cggcaaggcc gggatgactg
     1081 acattcacga gcaaacagcg tgccccctgc ggagcggcaa cgcgcatgcc gggagatggc
     1141 gttgcgccga accagcgcgg tgtcgcccga cgcgcgtcag ccggggaaga acggcttgcc
     1201 catcgaagcc ggcatgttga cgcggatgaa cgtcgcgttc ctcgagatga ggacgagcac
     1261 gacgatcgtg gccaggtacg gcaacatcgt caggaactgg ctcggcacct ggaccgcgcc
     1321 ggcgattccg ggcacgtgga aaccatcgga ctgctgcagc tggaactgca gcatcgtcac
     1381 gccgccgaac aggtacgcgc cgagcagcac ccgcgccggc cgccaggtcg caaaggtcgt
     1441 cagggcgagt gcgatccagc ccttgccggc gatcatcccc tcgacccaca gatgcgtgta
     1501 cacgaccgac acgtaggcgc cggcgacacc gcacagcgcg cccccgaacg ccaccgccag
     1561 cagcctgatc cgtcgcaccg gatagccgag cgcgtgcgcc gactcgggcg attcgccgat
     1621 cgcgcgcagc accagcccgg cccgcgaacg gtagaggaac caggccagcg ccgcggtcat
     1681 ggcgatcgcc aggtagacca tcggatgctg gcgaaaaagg gccggcccga agaacggcag
     1741 gtcggacagg ccgggcacat gaaagctcgc ccgctcggca agcctttgct gggtgaacgg
     1801 ctgccccacg aacgccgaga aaccggtgcc gaaaaggctc agcgccaggc cggtcgcgta
     1861 ctggttggtg ttcagccaga tgacgagcac gccgaacgcc gacgcgagca gggccccggc
     1921 aagcgcgccc gcgccgaatc cggcccagtc gctgccggtg tggaccgcga ccgcgaaacc
     1981 tgccaccgca gccaccagca tggtcccttc ggcgccgagg ttgacgatgc cggcgcgttc
     2041 gttgacgagc aggccgagcg cggcgaaggc gagcacggtg cccgaattga gcgtcgccgc
     2101 gatgagcagg gcggcttggt ccatcagctg cctttgcgcg ccggcaacgc cgacgtcgcg
     2161 acgacggggc tgaggggcgc gtcgtcgagc tccgaggtcg ccggcgccga ggcagccggg
     2221 ggcgacgccg acgacgacgt cgcggcagcc ccggcaacgc ccgtacgcca acgcacgcga
     2281 tacgcgatca gcgtgtcgca cgccagcagc gccagcagca acagcccctg gaacacgccg
     2341 gtgagcgact gcggcaggcc caacctcgat tgggagagct cgccgccgat gtagaacatg
     2401 ctcatcagga tcgccgagaa gacgatgccc accggatgca gccggccgac gaaggcgacg
     2461 atgatggccg cgaagccgta gccggcgggg acgtgcggcg tcagctggtt gagcggcccg
     2521 gccacctcga gcccgccggc gaggccggcc atgccgcccg agacgagcat cgtggtccac
     2581 agcgcccgcc gtgccgagaa accggcgtag cgcgccgccg cgggcgccag gccgccgacc
     2641 tgcaactgga accccgcgta ggtgcgaaag aggagcaccc agaatgcgcc gacggcgacg
     2701 agcgcgccca ccgcgccgat gttgatgcga taggtgtcga agagacgcgg cacctgggtc
     2761 gacttgagga aggtgacggt ctgcgggaag ttgtagccgt gcggatcctt ccatggcccg
     2821 tagacgaagt agttcagcaa ctgctcggcg acgtagacga gcatcaggct gaccaggatc
     2881 tcgctggcat tgaagcggtc gcgcaggaac gcgacgatcg ccgcccacag catgccgccg
     2941 accacgctgg cggcgagcac ggcgatgaag atccagtggc cggtgtcctt ggtcgcctgc
     3001 atcgcgacgc cgctggcgaa gatcgcgccg aggacgaact ggccctcggc gccgatgttc
     3061 cagatgttgg agcgaaagca caccgccagg cccagggcga tcagcagcag cggcgtcgcc
     3121 ttgatcgcca gctcggagag cgcgtaggca cccttcagcg gctcgacgaa gaagacgccg
     3181 agcccgcgca acggatcctt gccgagcacg acgaacagca gcgtgccgat gacgacggtg
     3241 atcgccagcg cgatcagcgg cgacgccatc gacatcgccc gtgaaggctc gggccgcgcc
     3301 tcaagccgca gcatgaccga gctccatcga tccggccggt gcggcgccgt gccagaggcc
     3361 gctcatccac tcgccgatcc tctcgaccga ggcgtcggcg acggcgacgc tcggcgagac
     3421 gcgaccctgg gcgatgacga cgagccgatc gcagatctcg aacagctcgt cgagctcctc
     3481 gctgacgacg aggacggccg agccgcggtc gcgaagcgcc agcagctcgc cgcggatctg
     3541 cgccgcggcg ccgacgtcga cgccccaggt cggctgcgac acgacgagca gcttcgggtc
     3601 ggcgtcgatc tcgcggccga cgatgaactt ctgcaggttg ccgccggaaa gacttcgcgc
     3661 cgccgagccc ggcccgccgg ccttgacgtt gaagcgacgg atcagcgtcg cggccaattc
     3721 ggtcactgca ccggtgtgga cccagcccga cgcggcgacc gcgccggtgc gcgtcagcag
     3781 ggtgttctgg gcgagcgaca aggtcggcac ggcgccgcgg ccgaggcgct cttcgggaat
     3841 gaaatgcagg ccctggcgac ggcggcggcg cggccggtca cgggcgatgt cgttgccgaa
     3901 cagccggatc gatccccgcg gtgcccgcac gtcctcgccc gacagcgcgc gcatcagctc
     3961 ctgctggccg ttgccggata cgccggcgat gccaagcacc tcgccggcgc gcagatcgaa
     4021 ggagatccgg tcgagcgacg tgccaaacgg gtcgtcgcgg gtcagcgaca gctcgcggac
     4081 ctcgagcacc acttcgcccg catgcgcctc gcgatgctgg agctgcggcg gctcggcgcc
     4141 gatcatcagc cgcgacaggc tggcattgct ctgcccggtc gggtcgacga cgccggtcac
     4201 ccggccgccg cgcaacaccg tgcagcgatg gcacagggca cggatctcgt cgagcttgtg
     4261 cgagatgtag aggatcgcgc agccctcggc ggcaagcttt ctcagcgtca cgaagagcgc
     4321 ctgcaccgcc tgcggcgtca gcaccgaggt cggctcgtcg aggatcagca gcttcggctc
     4381 gctcatcagc gcccgcacga tctcgacgcg ctggcactcg ccgaccgaca gcgtatgcac
     4441 gggccgcagc ggatcgatct cgagcccgta gacgccggcc accttgcgaa tgcgctgggt
     4501 tacctcggcc agcgggagcg agcggtcgag ccccagccag acgttctcgg cgacggtcag
     4561 cgtgtcgaac aggctgaagt gctgatagac catgctgatg ccgagcgcgc gcgccgccgc
     4621 cggcgtggcg atctgcaccg gcgcgccgtt ccagcggatc tctccttcgt cgggccgcac
     4681 cgcgccgtag atgatcttca tcagcgtcga cttgccggcg ccgttctcgc cgagcaccgc
     4741 gtggatctcg cccggctcga cgcgcaaggc gacgcgatcg ttcgccttca ccgaggggta
     4801 ctgcttgctg atgtcgacga gctcgaggcg aagcatgcgg cgggcgttct cagtgcgatc
     4861 cgcgatcgta gtgcctgcga ccggcgacga aagcggcggc gagattgcgt tcgtcggcga
     4921 gcgtcatcca cgcaaagacg cgctcgtgca ggcttcgcgc cacctcgagc cggcgttcgg
     4981 cgaccgggcc tgcggcccag tcccagacgc agagatcggc gatggttccc gtctcgagcg
     5041 aaccgatctc gccgccgagg tcgagcgacc gcgccgcgcc gcgcgtcgcc gcgtgcaacg
     5101 cctgccacgc cgtcagccgc tggccggcga gcgcctgcac cttgtaggcc tcggccatga
     5161 tgcgcggcat cgccaggctg gtgccgccgc cgacgtcgct cgccagcgag acgttcactc
     5221 ctgccgcatg ggccgcgcgc cagtcgaaca agccgctgcc gaggaacagg ttcgacgacg
     5281 gcgagtgggc gatctgcgcg ccggccgcgg cgagcatggc gcgatcggtg tcgtcgagcc
     5341 agacgccgtg cgcgaagatg ctgcgtcgat ggagcaggcc ggccgaggcg tagacgtcga
     5401 ggtagctgcg cgcgtcgggg aacagctcgg caacccagcg cagctcggcc cggttctcgg
     5461 cgagatgcgt ctgcaggtag aggctggcgt cgccggcgca caggcgcccg gccagcgtca
     5521 gttgttcggg cgagctcgtc ggcgcgaagc gcaccgtcac cgcatacgcg agccggccgc
     5581 ggccatgcca gcgggcgatc agctcggcca tgtcgcgctc ggcgctgtcg acgtcgtcgc
     5641 gcagatcgtc gggcgcattg cgatccatca gcaccttgcc ggcgacgatg cgcatgccgc
     5701 gcgactcggc ggcagcgaag agcgcatcga ccgagcgccg gtgcacggtc ggaaagacga
     5761 ccgccgaggt cgtgccgtgg gcgagcaagg cgtcgagaaa atgcgcggcg gcggcgtcgg
     5821 cgtgcgcagc gtcggcatga cgcgcctcgg ccggaaacgt gtgcgtcgtc agccagtcga
     5881 gcagctcggt gccgtagctg gcgatgacgt cgatctgggc gctgtgcacg tgcgtgtcga
     5941 tgaagccggg caggacgagc cggcccgcat ggtcgtgccg ctgccagcct tcgccggggt
     6001 cgccgcgctg gacgccggtg atgcggccgt cttctaccag cagccagtgg tcgggccgaa
     6061 atcgcagcgc cgccggctcg acgtcgcccc aggccggctc ggcggtgaaa tcgagcaggt
     6121 cgccgcgcac ggcgatgcgg gcgccagccg tcatgacggg cgcgccggcg caggcacggc
     6181 gagatcgcgg gccaccgcgc gaatctgttc gcgcagccat ttcgtcgacg ccgacgcgtg
     6241 cgtcacctcg tgccagagct ggtagtaggt gagcggcgga aaggcaatcg ggcagcgcac
     6301 gatcttcagc ggcaggctgt cgacgaaacg cgagcagaaa agccggccgg tggtcaggac
     6361 gagccggctt tgcgccacca tcaacgggat caggctgaaa tgagcgctct tcacggcgac
     6421 gtcgcgggcc agcccgagcg atgccaggtg gtcgtcgacg acgccgggtg cattggcgtg
     6481 aaacggcatc ggggcgacgt gctcggccgc gagatagcgg tcggccgacc agccccggcg
     6541 cgtcgccggg tggtctgccg cgaccaggca caccacctcg tcgctgaaga ggcgcccgag
     6601 atgcagctcg ccgggcggcc gcaaccagtt gccgacgacc aggtcgacct cgccggcggc
     6661 cagccgttgt cgatagtcgt attcggcgct gagcggcagc agctcgagac ggatcgaagg
     6721 cgcctgccgc ttgacgtggc cgacgatccc gggaagaaac gacggatcga ggtagtcgct
     6781 ggccgcgatc cgcagcgtcg cctcgctcgc cgccgggtcg aagccgcgct ggcgcgcccg
     6841 cgcgccgaac aagcgctcgg cctcgtcgag cagcgccgcc gccggcccga gaagccgaag
     6901 cgcggtgtcg gtcggcgtca tccggttgcc ggcgcgcacc agcagcgcgt cgccggtgag
     6961 gccgcgcagg cgtcgcagct gggcgctcac cgccggctgg ctcgacccga gtcgcatggc
     7021 ggcacgcgag acgcttcgtt cgctgatcac ggtatgcagc acccgcacga gttggagctc
     7081 gatcttcccg aatgcgctct ctgacggcat acgtcgagga tgatatgtgc catcgaagcg
     7141 gctgtatatc atcggacgga cggcaggagt accttcccag cctgcgagcc catgtgatgt
     7201 cgagccgtcc gatccgcttc ttccaccgcg gcgccgtcgt cgagatcgac gccgcggcga
     7261 cgacgcgcac cgtcctcgac tggttgcgcg aagacgcgca ctgcaccggc accaaggagg
     7321 gctgcaacga gggcgattgc ggcgcctgca cggtggtcat cggcgaaatc gcgggccgcg
     7381 aagcgaagtc ggcccccgac gtcgtcgacg gcctgcgtct gcgcagcgcc aatgcctgcc
     7441 tgcagttcct gccgacgctc gacggcaagg cgctgttcac ggtcgaggac ctggccactg
     7501 ccgtcgtcgc ggcgccgcac cggcatgccg atgggctgcg ccatccgccc caggcgccgg
     7561 atcgcgcgcc gggcgcaagt ggcgaagctg gcgccgtcgc cgcgaaagcc ggggccgccg
     7621 aagtcggggc ggcctgcgcg gcgagcgctt cgctccaccc ggtgcaacgc gccatggtcg
     7681 actgccacgc ctcgcaatgc ggcttctgca cgccgggctt cgtcatgtcg ctctgggcga
     7741 tgtaccagca tcacctggcc agcgcgaccc ggcccagccg gcagcagatc gccgacgagc
     7801 tctcgggcaa cctgtgccgc tgcaccggct accggccgat cgtcgacgcc ggcgagcgga
     7861 tgttcgacgc cccccctgcc cggctcgacg cgcgcgccgt cgtcgccgcg ctcgaaggcc
     7921 tgaacacgac ggcggcgccc gatttcgtct cgcccggcgg cgacatcttc ctcgcgccgc
     7981 acagcctcga cgaattcgct cgccagcggc tggcccggcc cgaggcacgc gttctcgccg
     8041 gtgccaccga catcggcctg tgggtgaaca agatgttccg cccgcttccc gagctgctct
     8101 acatcggcca cgtcgccgag ctgaagcgca tcgaagagcg cgaccacgtg ctctcgatcg
     8161 gcgccgcggc ctcgctcgaa gacgcctggc gttccctggt cgcccgttgg ccgagcctgg
     8221 ccgaggtctg gctgcgcttc gcgggcgtgc ccttgcggca caccggaacc atgggcggca
     8281 acgtcgccaa cggctcaccc atcggcgact cggcgccggt gctgatggcc ctcggcgcca
     8341 gcctgctgct gcgccagggc gaccggctgc ggcgcctgcc cctgagcgag ttctacaccg
     8401 gctacatgac gaaccggctc gaggccggcg agttcctcca ggccatcgaa gtgccgctcg
     8461 acacgagggc atcggtacgt gcgtggaaga tctcgaagcg cttcgattgc gacatctcgg
     8521 ccgtctgcgc cgggctcgcc atcgagctcg acgaagccgg ctcggtcgtc gccgcacgct
     8581 tcgccttcgg cggcatggcg gcgaccgcca tgcgtgccgc cggtgccgag cgggccgtgc
     8641 tcggccgcgc ctggaacgag gccaccgtgg tcgccgcgca aacggcgctc gacgccgatt
     8701 tcgctccgct caccgacgtg cggggcggcg ccggctatcg gcgtctcgtg gcgaggaacc
     8761 tgcttcgccg cctctggctc gagacgcgcc gcgagacgcc gctggcgagc acgcaaacgc
     8821 gcgtctggca ggtccttcca cccgtcgcga cccccgcgcg cgccgacacg ttcatcggtg
     8881 cgccgccatg aatcgcgccc tcgacgcgtc ctggctggcg ccgacggaca gcggcgacgc
     8941 gcaagcgctc ggcgccggcg cgcgcgtcgg catttcacgg ccgcacgaat cggcgcacct
     9001 gcacgtcgcc ggcgacgcac cctacgtcga cgaccttccc gagctcgccg gcacgttgca
     9061 cgcggcgctc ggcctgtcgc cggtcgcgca cggccggatc gtcgccctcg acctggccgg
     9121 catcgcggcg ctgcccggcg tcgtcgcggt gctgacggcg cgcgacatcc ccgggccgaa
     9181 cgattgcgga gcggtcgttc acgacgatcc catcctcgcc gacggcatcg tccactacgt
     9241 cggccagccg gtcttcgccg tgatcgcgac cgatcggctc gccgcgcgcc gggccgccgc
     9301 cgaagccaag tccgcgctgc ggatcgagcc gctcccggcg ctgctgacgc cggtcgaggc
     9361 ccacgctgcg gccagctacg tgctgccgcc gatgcacctg gtgcgcggcg atgcgcgcgc
     9421 cgccatcgac tcggcgccgc accgtttcac aggctcgctc gaagtgggcg gccaggagca
     9481 gttctacctc gaaggccaga tctcgtacgc ggtgccgcgc gaggatcgcg gcatgctcgt
     9541 gcattgctcg acccagcatc cgagcgagat gcagcatcac gtcgccagtt gcctcggcct
     9601 gtcgtcgcac cacgtgcagg tcgagtgccg gcgcatgggc ggcggcttcg gcggcaagga
     9661 atcgcaatcg gcgctcttcg cctgcgtcgc cgccgtcgcc gcgcagcgcc tgcaggtgcc
     9721 ggtgaagctg cgcctcgacc gcgacgacga cttcctcgtc accggtcgac gccattgctt
     9781 ccactacgag tacgaggtgg gccacgacga cgagggccgc atcctcggcg ccgagctgac
     9841 gatggtctcg cgcgccggct tctcggccga cctgtccggg ccggtgatga cacgcgccgt
     9901 ctgccacttc gacaacgcct actggctgcc cgacgtcgcc atccacggct actcggcgcg
     9961 cacgaacacg caaagcaaca ccgcctttcg cggcttcggc ggcccacagg gcgcgatcgc
    10021 ggtcgagaac atcctcgaca cggtcgcgcg tcgcctcggc aaggatccgc tcgatgtccg
    10081 ccggctcaac ttctacgggc cgagtgcaag cgccgcaggc cctggcgagc gcaatgtgac
    10141 gccgtacgga cagacggtcg aggacaacat cctgcacgag ctcgtcggcg agctcgaggc
    10201 gaccagccgc tatcgcgagc ggcgggcgca ggtcgacgcg ttcaacgccg gcagcgaggt
    10261 gctcaagcgc ggcctggcgc tgacgccggt gaagttcggc atctccttca atctcgtcca
    10321 cctcaaccag gccggcgcgc tcgtccacgt ctatggcgac ggctcggtgc tcgtcaacca
    10381 cggcggcacc gagatgggcc aggggctcaa caccaaggtc gctcaggtcg tcgcccacga
    10441 gctgggggtc gacttcgacg cagtgcgcgt gacggcgacc gacacccaca aggttgccaa
    10501 cacctcggcc accgcggcct cgaccggcag cgacctgaac ggcaaggcgg cgcaggacgc
    10561 ggcccggcag atcaaggcac gtctggtcgc cttcgcggcc gagcgctacg gcgtcgaggc
    10621 cagcgccgtg cggttcgcca acggctcggt gtcgatcggc acgacccgga cactcggctt
    10681 tcgcgagctc gtcgtcgagg cctacatggc gcgcgtgcag ctctggtcgg acggcttcta
    10741 cgcgaccccc ggcctctcgt gggacgccgc gacgatgcgc gggcggcctt tcttctactt
    10801 cgcctacggc gcggcggtga gcgaggtcgt cgtcgacacg ctgaccggcg agtggaagct
    10861 gctcgccgcg catgtgcttc acgacgccgg ccgctcgctc aacccggcga tcgacctcgg
    10921 ccaggtcgag ggcgggttca tccagggcat gggctggctg acgaccgaag agctcgtctg
    10981 gcatccggac gacggaacga gccgggccgg attgctgctc acccacgcgc cgagcacgta
    11041 caagatcccg accgccaacg actgcccgcc ggtcttcgac gtgtgcctgt ggaaggacgg
    11101 actgggcgcc ggcgacaacg tca
//
LOCUS       DASRWS010000637         7076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1966708, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000637 DASRWS010000000
VERSION     DASRWS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966708"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="cysS"
                     /locus_tag="VFF72_12135"
     CDS             <1..1192
                     /gene="cysS"
                     /locus_tag="VFF72_12135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZV93960.1"
                     /translation="TYVRNITDIDDKIIRRSLERGISIRALTEEMTVALHEDAAALGI
                     EPPTHEPRATQYVAQMLDMIAVLEKKGLGYRAANGDVNFSVRRFPGYGKLSGKSIDQL
                     RAGERVAVLDGKEDPLDFVMWKSAKPDEPEDAKWTGEFGAGRPGWHIECSAMCKALLG
                     EHFDIHGGGQDLQFPHHENEIAQSEGASGGPFANVWMHNGLLNVDNEKMSKSLGNFFT
                     IRDVLKRYDGETIRFSMLRTHYRSPFNFSDAQLDDARNALRRLYTALGTVAEMPRSPI
                     DWNEPRASAFREAMNDDFNTPIATSVLFELAAEVNRHHRPSDAALLRELGRVLGVLQQ
                     APQSYLQGGAGLVEADIVRLIEERRAAKAAGDYSRADAIRDDLAKQGVILQDSAAATT
                     WVRA"
     gene            1220..1858
                     /locus_tag="VFF72_12140"
     CDS             1220..1858
                     /locus_tag="VFF72_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZV93961.1"
                     /translation="MAPPSADFWDDACRHLSRRDRVMKKLIPRFSEGRLESRGDAFTT
                     LARSIVGQQISVKAAQAVWDRLLALTTGPSQRLSAAAVLALDAPRIRTAGLSARKVEY
                     LCDLAQHFDSGTVHVAEWREMEDEAIIEELVAIRGIGRWTAEMFLIFHLLRPNVLPLD
                     DVGLIKGISVNYFSGEPVSRAEARELGEAWAPYRSVATWYIWRSLDPVPVSY"
     gene            1938..2909
                     /locus_tag="VFF72_12145"
     CDS             1938..2909
                     /locus_tag="VFF72_12145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZV93962.1"
                     /translation="MSKRHFLDFEQPVADLESKIDELRYVQNESAVDISEEIDRLSKK
                     SLLLTKDIYSGLTPWQVTQIARHPQRPYTLDYVAEIFTDFQELHGDRAFADDQSIIGG
                     LARFNGQSCVVIGQQKGRDTKERAARNFGMSRPEGYRKALRLMKLAEKFGMPVFTFVD
                     TPGAYPGIGAEERGQSEAIGRNIFEMAQLEVPIIVTIIGEGGSGGALAISVGDQLVML
                     QYSIYSVISPEGCASILWKTAERAADAAEQLGITAHRLKALGLVDKIVNEPVGGAHRD
                     PKQMAAFLKRALNDAFRQVSDLSVRDLMQRRYQRLQSYGRFTDTTER"
     gene            2935..4212
                     /gene="tilS"
                     /locus_tag="VFF72_12150"
     CDS             2935..4212
                     /gene="tilS"
                     /locus_tag="VFF72_12150"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZV93963.1"
                     /translation="MAVAFSGGRDSTALLHATVQHAQLAGIEVLALHVHHGLSVHADA
                     WLRHVQTLCRRWARRGKPVRLVAEILDTRPRRGESVEAWARRERYRALVRMANAEDVD
                     LVLLAHHRRDQAETFLLQSLRGGGVAALSSMPSIVRREGILWARPWLATSSEAIDNYV
                     RAHRLRHVVDDTNDDARFARNRLRATVWPALQAAFPDVEATLAAAAQRVQEAAAALDE
                     WARIDLANVEVDGALDIRAWRALSPARQRATLRAWLIRNRVEASHHLLDRLGREIDAR
                     RTGRWPVDEGELRGHRGRLRMFPGPFPLPLVAPGCTVRLDLRRPGLHRVPGWPGQIAV
                     ETVTSGGLPVSVAAEVDLRARQPGDRFQSGAGRPARSLKLQYQAAGLPAWERCGPIGC
                     SAAQVVFVAGLGIDARAIARPGQPQVTLTWLPD"
     gene            4324..5607
                     /locus_tag="VFF72_12155"
     CDS             4324..5607
                     /locus_tag="VFF72_12155"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZV93964.1"
                     /translation="MSLIVHKYGGTSMGSPERIQSVARRVAKWSRAGHRMIVVPSAMS
                     GETNRLLALAKQLAPERSTPEMLRELDVIAATGEQVSVGLLAIALQAEGLAAVSYAGW
                     QVPIATDVAYTKARIQHIDDQRIRTDLAAGKVVVIAGFQGVDPDGHITTLGRGGSDTS
                     AVAVAAALKADECLIYTDVDGVYTTDPRIVADARRLTSISFEEMLEMASLGSKVLQIR
                     SVEFAGKYRVPLRVLSSFTPWDIPLDDEARSGTLITFEEDAKMEQAVVSGIAFSRDEA
                     KISLMGVTDKPGIAHLILGPVAEANIDVDVIIQNVSHDGRTDFSFTVNRGDYARTMDL
                     IRTAVIPKTGAAGLTGDDRICKVSIVGIGMRSHVGVASRMFSVLSEEGINIQMITTSE
                     IKTSVVIDEKYMELAVRALHKAFDLDQEPKASAAS"
     gene            5654..5746
                     /locus_tag="VFF72_12160"
     tRNA            5654..5746
                     /locus_tag="VFF72_12160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5688..5690,aa:Ser,seq:gct)
     gene            5932..6138
                     /locus_tag="VFF72_12165"
                     /pseudo
     CDS             5932..6138
                     /locus_tag="VFF72_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6469..>7076
                     /locus_tag="VFF72_12170"
     CDS             6469..>7076
                     /locus_tag="VFF72_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZV93965.1"
                     /translation="MLDYATARGYREGLNPARWKGNLDAALPNASKVAPVRHHAAVAV
                     KDAPSFIKRLRAQQGMGARALEFVMLTAARSGEVRGATWSEVDLEAALWTVPASRMKS
                     GREHRVPLSKEAIGLLGTLPGDHLPDDYLFPGMRGPLSDMSLTAVLRRMKVAATAHGF
                     RSTFRDWVSEHTEYSSEVAEMALSHAIGDKVEAAYRRGDLLA"
BASE COUNT         1249 a         2307 c         2314 g         1206 t
ORIGIN      
        1 cacatacgtc cgcaacatca ccgacatcga cgacaagatc atccggcgtt cgctcgaacg
       61 aggcatctcg atccgcgcct tgaccgagga gatgaccgtc gcgttgcatg aggatgccgc
      121 tgccctgggc atcgagccgc cgacgcacga gccgcgggcg acgcagtacg tggcgcagat
      181 gctcgacatg atcgcagtcc tcgagaagaa gggcctcggc tatcgggctg cgaatggcga
      241 tgtcaacttc tccgtgcgac ggtttcccgg ctatggaaag ctttcgggca agtcgatcga
      301 ccagctgcgc gccggcgagc gggtggccgt gctcgatggc aaggaagacc ccctcgactt
      361 cgtgatgtgg aagagcgcca aaccggacga gcccgaggat gcgaaatgga ccggcgaatt
      421 cggcgccggt cggccgggct ggcacatcga gtgctcggcc atgtgcaagg cgttgctcgg
      481 cgagcatttc gatatccacg gcggcggcca ggatctgcag tttccccatc acgaaaacga
      541 gattgcgcaa agcgagggtg ccagcggcgg ccccttcgcc aacgtctgga tgcacaacgg
      601 gctgctcaac gtcgacaacg agaagatgtc gaagtcgctg ggcaacttct tcaccatccg
      661 cgacgtgctc aagcgctacg acggcgagac gatccgcttc tcgatgttgc gcacgcacta
      721 ccgcagtccg ttcaacttca gcgacgccca gctcgacgat gcgcgcaatg cgcttcgccg
      781 cctgtacacg gccctcggca cggtcgcgga gatgccgcgt tcgccgatcg actggaacga
      841 gccccgcgca agtgcctttc gcgaggcgat gaacgacgac ttcaatacgc cgatcgccac
      901 ctcggtgctg ttcgagcttg ccgccgaagt gaatcggcat catcgaccgt ccgacgcggc
      961 cctgttgcgc gagctggggc gcgttctggg cgtgctgcaa caagcgccgc agtcttatct
     1021 gcagggcggt gcggggctcg tcgaggcgga catcgtccgc ctgatcgagg aacggcgcgc
     1081 tgccaaggcg gcaggcgact attcccgtgc cgacgcgatc cgggacgacc tggcgaagca
     1141 gggcgtcatc ctccaggatt cggccgccgc gacgacctgg gtgcgggcct agaccttggc
     1201 gacgatttcc aggccatcga tggcgccgcc gagcgccgac ttctgggacg acgcctgccg
     1261 gcatctgtca cggcgcgatc gcgtgatgaa aaaactcatc ccgcgctttt cggaaggcag
     1321 gctggagagc cgcggcgatg ccttcacgac gctggcgcgc tcgatcgtcg gccagcagat
     1381 ctcggtcaag gccgcccagg cggtctggga ccggctgctc gcgctgacca cgggaccctc
     1441 gcaacgactg tcggcggcgg ccgtgctcgc cctcgacgcg ccgcgaatcc gcaccgcggg
     1501 cctctcggcg cgcaaggtcg agtacctctg cgatctggcc cagcacttcg attcgggcac
     1561 cgtccatgtc gccgagtggc gcgagatgga ggatgaagcg atcatcgagg agctcgtggc
     1621 gatccgcggc atcggtcgct ggacggcgga gatgttcctc atcttccatc tgcttcggcc
     1681 caatgttctt ccgctcgacg acgtcggcct catcaaggga atcagtgtca actacttcag
     1741 cggcgagccg gtgtcgcgtg ccgaggcgag ggaactcggc gaggcctggg cgccgtaccg
     1801 gtcggtcgca acttggtaca tttggcgcag tctcgatccg gtcccggtca gctattgaat
     1861 gattccgagc ccttggctgc attcgcgcct cgccgcttcc actccggggg tctcgcaacc
     1921 ctcgcgcgcg cactgacatg agcaagagac atttcctgga tttcgagcag cccgtggccg
     1981 acctcgagtc gaagatcgac gagttgcgct acgtgcagaa cgaatccgcg gtcgacatct
     2041 ccgaagagat cgaccggctc agcaagaaga gcctgctcct cacgaaagac atttattcgg
     2101 gcctgacgcc gtggcaggtc acgcagattg cgcggcaccc ccagcggccc tacacgctcg
     2161 actacgtggc cgagatcttc accgacttcc aggaattgca cggagaccgt gcgttcgccg
     2221 acgatcagtc gatcatcggc ggcctggcgc gcttcaacgg ccagtcctgc gtcgtgatcg
     2281 gccagcaaaa gggtcgcgac acgaaggaga gggcggcccg caatttcggc atgtcgcggc
     2341 ccgagggtta tcgcaaggcg ctgcggctca tgaagctggc cgaaaagttc ggtatgccgg
     2401 ttttcacctt cgtcgacacg ccgggcgcct accccggcat cggcgccgag gagcgcggcc
     2461 aatcggaagc gatcggacgc aacatcttcg agatggcgca gctcgaagtg cccatcatcg
     2521 tcacgatcat cggcgaaggc ggctccggcg gcgcgctggc gatcagcgtg ggcgatcagc
     2581 tggtgatgct tcaatactcc atctattcgg tgatctcgcc cgagggctgc gcctcgatcc
     2641 tctggaagac ggccgaacgg gcggccgatg cggccgagca actcggcatc accgcgcatc
     2701 gtctgaaggc gctcgggctc gtcgacaaga tcgtcaacga gcccgtcggc ggcgcgcacc
     2761 gcgatcccaa gcagatggcc gccttcctga agcgcgcgct caacgatgcg ttccgccagg
     2821 tgagcgacct gtcggtgcgc gacttgatgc agcgtcgcta ccagcggctt cagtcgtacg
     2881 gccgtttcac cgacaccacc gagcgctgac gcgagcgagc ccgcgcctcg gcgggtggcc
     2941 gtcgccttca gcggtggccg cgattcgacg gcactgctgc acgccaccgt gcagcatgcg
     3001 cagctggcgg gcatcgaggt gctggcgctg cacgtccatc atggcctgag cgttcacgcc
     3061 gatgcgtggc tgcgacatgt ccagacgctg tgccggcgct gggcacgacg gggcaagccg
     3121 gtccgccttg tcgccgagat cctcgacacg cggccccggc gtggcgaaag cgtcgaggca
     3181 tgggcgaggc gggagcgcta tcgcgcgctc gttcgaatgg ccaatgccga ggatgtcgac
     3241 ctcgtccttc tcgcgcatca tcgccgcgac caggccgaaa ccttcttgct gcagtccttg
     3301 cggggtggag gcgttgcggc gctctcgtcg atgccatcga tcgtccgccg cgaaggcatt
     3361 ctctgggcac gaccgtggct cgcgacttcg tcggaggcga tcgacaacta cgttcgtgcg
     3421 catcggcttc gtcacgtcgt cgacgacacg aacgacgatg cccgcttcgc acgcaatcgg
     3481 ctgcgggcga cggtctggcc ggcgctgcag gccgcctttc ccgatgtcga ggcaacgctg
     3541 gcggccgcag cgcaacgcgt gcaggaagcg gcggctgcgc tcgacgagtg ggcgcgaatc
     3601 gatcttgcga acgtcgaggt cgatggagcc ttggacatcc gcgcctggcg ggcgctgtcc
     3661 ccggcacgac agcgcgcgac gctgcgtgcc tggctcattc gcaatcgtgt cgaagccagc
     3721 caccatctgc tggatcggct cggtcgcgag atcgacgctc gccgcaccgg gcggtggccg
     3781 gtcgatgagg gggagctgcg cggccaccga ggccggctgc ggatgtttcc cgggcccttc
     3841 ccattgccat tggttgcacc ggggtgcacg gtccggctcg atcttcgccg accgggcctg
     3901 catcgcgtgc caggttggcc aggtcagatc gccgtcgaga cggtcacgag tggcggcttg
     3961 cccgtgtcag ttgccgccga ggtcgatctt cgcgctcgcc agccggggga tcgcttccag
     4021 agcggcgccg gtcgaccggc acgcagcctc aagctgcaat accaggcggc cggcctgcct
     4081 gcctgggaac gttgtggtcc gatcggttgt tcggcggcgc aagtggtctt cgtcgccggt
     4141 ctcggcatcg atgcccgcgc gatcgcgcga ccgggtcagc ctcaggtcac gctgacctgg
     4201 ctgcccgatt gatcggattc gcgtcgctgc gtgcaaggct cgcgtcgata gaatcatcgg
     4261 ctcgcttcct ctcattcctg cgtccgtcgc cgtcactgcg ggctcgaccg cggcgcaaca
     4321 tccatgtcac tgatcgttca caagtacggc ggtacctcga tggggtcgcc ggagcggatc
     4381 cagagcgttg cccgtcgcgt tgccaagtgg tcgcgagccg gccaccgcat gatcgtcgtt
     4441 ccttcggcga tgagcggcga gaccaaccgc ctgctcgccc tggcgaagca acttgcgccc
     4501 gagcgaagca cgcccgagat gcttcgcgag ctcgacgtga tcgccgcgac cggcgaacag
     4561 gtctcggtcg gactgctcgc gatcgcattg caggccgaag ggctcgccgc agtgagctat
     4621 gccggctggc aagtaccgat cgccaccgac gtcgcctaca cgaaggcgcg aatccagcac
     4681 atcgacgacc agcgcatacg caccgatctc gccgccggca aggtggtcgt catcgccggg
     4741 ttccagggcg tcgatccgga cggtcacatc acgacgctcg gccgaggcgg ctccgatacc
     4801 tcggcggtcg ccgtcgcggc ggcgctgaag gccgacgaat gcctcatcta caccgacgtc
     4861 gacggcgtgt acaccaccga cccgcgcatc gtcgccgacg cgcgtcgtct gacgagcatc
     4921 agcttcgagg agatgctcga gatggcgagc ctgggctcca aggtgctgca gatccgatcg
     4981 gtcgagttcg cgggcaagta ccgcgtgccg ctgcgggtgc tttcgagctt cacgccctgg
     5041 gacattccgc tcgacgacga agcccgctcg ggcacgctca tcaccttcga ggaagacgcg
     5101 aaaatggaac aagccgtcgt atccggcatc gccttcagcc gagacgaggc gaagatcagt
     5161 ctcatgggcg tgaccgacaa gccaggcatc gcccatctca ttctcggccc cgtcgccgaa
     5221 gccaacatcg acgtcgacgt gatcatccag aacgtctcgc acgacggccg caccgacttc
     5281 tccttcaccg tcaatcgcgg cgactacgcg cggacgatgg acctcatccg gaccgccgtc
     5341 attccgaaga ccggtgctgc cggattgacc ggcgacgacc gcatctgcaa ggtgtccatc
     5401 gtcggcatcg gcatgcgctc gcatgtcggc gtcgccagcc ggatgttcag cgtgctcagc
     5461 gaggagggca tcaacatcca gatgatcacc acgagcgaga tcaagaccag cgtcgtcatc
     5521 gacgagaagt acatggagct tgccgtgcgc gcactgcaca aggcgttcga tctggaccag
     5581 gagccgaagg catcggcagc gtcgtgagag gagacgctcg cgtggaggcg agccgctaga
     5641 atcgcccccc aacggagctg tgaccgagtg gccgaaggtg ctcccctgct aagggagtat
     5701 gtgggcaaaa cctgcatcga gggttcgaat ccctccggct ccgccaagct agcctctgac
     5761 tgaagccccg aaaggggctt tttcataggt agaatcacaa aacactgtcc cctggcgtcc
     5821 atcgacatcc gaagacgtac ccctcaaagt gagggattag tgaggggtaa gctcagtatg
     5881 gcggactctt tgaaatgctt gggaaaaccc tgagatcgac gagagggtca catgccccga
     5941 aaggccaaag agctctcgtc gcttgaggtc cgccggctta cccggccggg ccgatggagc
     6001 gtcggtggcg ttgacggcct gggtcttcag gtgacgcaat ccgggacgca aagctgggtg
     6061 cttcgtttca ggatcggtgc ccggcagcga gagatggggc tcggcagttt tcccaccgtc
     6121 ggccttgccg cggcacgtga gaaggcgcgg caacttcgat cgatcgtcag tgacgggggc
     6181 gatccgatcg cgatcgttca ggcaagagac agcgctgcgc ttgccgaacg ccacacccag
     6241 aagacgtttt ctgaggttgc ggagcgatac atcgcccagc atgagaggtc gtggaagaac
     6301 gcgaagcacc aggcgcaatg ggcggcaacg cttcgtacat atgcggagcc atcgatcggc
     6361 aagttgcttg tcaaggacat ccggccggcg cacgtcattg gcgtcctcga gcccatctgg
     6421 atcaccaaga ccgaaaccgc cacgcgtgtt cgctcgcgca tcgaactcgt gctcgactac
     6481 gccacggcgc gtggctaccg ggagggcctc aatccggcgc gctggaaagg aaatctcgac
     6541 gccgccctgc cgaacgcctc caaggtcgca cctgttcggc atcatgccgc tgtcgccgta
     6601 aaggatgcgc cgtcgttcat caagcggctt agagctcagc aagggatggg cgcgcgcgcg
     6661 ctcgagttcg tgatgctgac ggccgcgcga tcgggcgagg tacgcggagc cacgtggtcc
     6721 gaagttgatc tcgaggcggc gctctggacc gttcccgcat cgcggatgaa gtcgggtcgc
     6781 gagcatcgtg ttccgctttc gaaagaggct atcgggctgc tgggcacgct ccctggagat
     6841 cacctgcccg acgattacct gttccccggg atgcgaggcc cgctctccga catgagcctg
     6901 acggccgtgc tacgacggat gaaggtggcc gccaccgcgc atggtttccg cagtacgttt
     6961 cgcgattggg tgtcggagca caccgagtac tccagcgagg tcgccgaaat ggcgctttcg
     7021 catgccatcg gtgacaaggt cgaggcagcc taccgacgtg gcgacttgct cgcaaa
//
LOCUS       DASRWS010000638         6147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4265072, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000638 DASRWS010000000
VERSION     DASRWS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4265072"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFF72_12175"
     CDS             complement(<1..333)
                     /locus_tag="VFF72_12175"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZV93966.1"
                     /translation="MTAAPAKKKLRSSIVTEGLDRAPHRAFLRATGVADEDMDKPFVG
                     IVSQHGENTPCSMSLGPQADAARLGVAAGGATPVQFTTVSVSDGVSMNHKGMRMSLVS
                     RELIADSIE"
     gene            complement(330..1307)
                     /locus_tag="VFF72_12180"
     CDS             complement(330..1307)
                     /locus_tag="VFF72_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZV93967.1"
                     /translation="MRTLVTGAGGFIGRGLVERLVADLVAKPNAATLCLTDLDLSGAG
                     DGSTTGDPRIVGIEGDIVDAPLQARLFAEPIDAVFHLAGVVSGAAERDFGLGKRVNLD
                     ATIALLEHCRQQAERGGPLVRFTYASSIAVFGAPLPGRIDDATPAVPGLSYGAHKRAC
                     EVLIDDYTRRGFIDGRALRLPGVVVRPRTPNGALSAFNSDLIREPLAGRDYVCPVGRD
                     ATLWVVSRWRAVDALRRLAAIDGEALGRVRAVNMPALRVEVGAIVEALARAKPGSETH
                     VRFEPQVALEAQFARWPLAASFDRGQTLGLCADTSLDEVITRHLKDDRP"
     gene            complement(1307..2260)
                     /locus_tag="VFF72_12185"
     CDS             complement(1307..2260)
                     /locus_tag="VFF72_12185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV93968.1"
                     /translation="MCQSIPRAVVGFIGLGVMGGPMAGHLSRAGHALRLFDLAPGRAA
                     GIAASLEGARAFDSPAALARECDVVVTMVPNGEVVHSLITGDDGLLHGMAPGSLLLDT
                     SSCEPWLTERSAALLASRGVAMVDAPVSGAQWGAEAAELVFMVGGAAADVERVRPLLE
                     VMGQAVFHVGGTGAGHAMKCLNNLVTAVNFLAVSEGLVVGKRYGLDPAAMVDVLDVST
                     GMSWISKTHIRQRVISRRFDDPFKLALMLKDVGIALELGRSTGSVTPLCALAQQLWRA
                     AATHSESEASVSELARWVEQLNGTEIRAGAATPPADAGSGG"
     gene            2410..3444
                     /locus_tag="VFF72_12190"
     CDS             2410..3444
                     /locus_tag="VFF72_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZV93969.1"
                     /translation="MTASRRTLLLGAAAIAAAPRVVLAASTTTLTMSSWVGPNHLLTR
                     DVLQGWATSVEKATNGRVKFEMLAKHPSSPPGTFDAVRDDLVDVSYVTASYTPARHPL
                     PLLAELPGAGATAEINSVAYSRIHWKYLEAAHEYKEVKLLGVFTHGPGQMFMVKKPIH
                     AVADLAGLKIRTGGGIAEAVARALGASPFVKPAPESYELLSSGIADGTFFPSESIKSF
                     NLDKVVKYATFFPGGLYSSAFGFFMNLDKWNKLPKQDQDAITSVSGEVLARMAGKAWD
                     AADKVGLAAMKAAGVQITEASPQFIADEHRLTAPLVDKWIKSADAKGIDGAKVYAAFH
                     EELKKVAAGQ"
     gene            3488..4219
                     /locus_tag="VFF72_12195"
     CDS             3488..4219
                     /locus_tag="VFF72_12195"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HZV93970.1"
                     /translation="MSGEDPRPALTRSARILADEGHESGLAGQLTARGPKPGTFWTLP
                     LGLAFDEAGESSWLLIDDELRVLEGPGSRGASAAIAEPNPATRFHLWVYRARADVHSI
                     VHTHPPAASALSAAEQPLIVAHMDATPLFDDIAFLGKWPGLPTADREGEVIAGGLGDK
                     HAILLAHHGLLAAGRSVQEATFLAVFIERMARQQIDAAAVGGSRPVDADEARRARDFL
                     RTDKIMNLTFDAWARRADRRRAGPQ"
     gene            4216..4722
                     /locus_tag="VFF72_12200"
     CDS             4216..4722
                     /locus_tag="VFF72_12200"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZV93971.1"
                     /translation="MNRVDVWLQRVLGIVAAVVLFLMMTITATDVIGRYVFNKPINGG
                     FELTELLLATLIYCGLPLVSARREHIVIDTFDPFFSVRFKHALDVIAEVACAVAFTGA
                     GALVFLRAVRVANYGDTTSVLHIPLAPVAYLMAAMIVVAALVHLWLVFAPRPATGPRT
                     TPESGASA"
     gene            4719..6014
                     /locus_tag="VFF72_12205"
     CDS             4719..6014
                     /locus_tag="VFF72_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZV93972.1"
                     /translation="MTESLIALAAMLVLSFLRLPIAIAMGGVGFLGLWWMRGLHPAMA
                     SATTVLYESGFEYSLTVVPLFILMGSFVTRAGMSKELYRAAHTFVGHRRGGLAMATVL
                     ACAGFGAICGSSIATAATMTRVAYPSMRDNGYSEELAVGSIAAGGTLGILIPPSTIMV
                     LYGILTETNIGKLFAAGVLPGLIETFVLCLAVQYMCWRNPAAGPRGPRIGWAERGRAL
                     RDVWAVAALFGVVMGGIYGGVFTATEGAGVGAFGAFLFALARRSLDWAGLLDVLVDSA
                     RTTSMLFIILVGALIFSNFVDYTSMPADLKSFVTDHHLHPLTVVAAICFIYIVLGTAM
                     EELSVILLTIPIFFPLVIGLGFDPVWFGIIIVVVMQIGLISPPVGMNMFVVKSLLPGV
                     STRAIFRGVTPFVFAEILVLALLVAVPWISVVLPGLLKL"
BASE COUNT          875 a         2215 c         2096 g          961 t
ORIGIN      
        1 ctcgatcgag tcggcgatca gctcgcgcga gaccaggctc atgcgcatgc ccttgtggtt
       61 catcgagaca ccgtcggaga cagagacggt tgtgaactgc accggcgttg cgccgccggc
      121 ggcgacgccg agtcgcgccg cgtcggcctg cggcccgagc gacatcgagc acggcgtgtt
      181 ctcgccgtgc tgactgacga tgccgacgaa aggcttgtcc atgtcttcgt cggcgacgcc
      241 ggtggcgcgc aggaaggcgc ggtgcggggc acgatcgagc ccttcggtga cgatcgacga
      301 gcggagcttc ttcttcgccg gtgctgcggt catgggcggt cgtccttcag gtggcgggtg
      361 atgacttcgt cgagcgacgt gtcggcgcaa aggccgagcg tctgcccgcg atcgaacgac
      421 gcggccagcg gccagcgcgc gaactgcgcc tcgagcgcga cctgcggctc gaagcggacg
      481 tgcgtttcgc tgccgggctt cgcccgcgcc agcgcctcga cgatggcgcc gacctcgacg
      541 cgaagcgccg gcatgttgac ggcgcggacg cggccgagcg cctcgccgtc gatggcggcg
      601 agacggcgca aggcgtcgac cgcgcgccat cgcgagacga cccacagcgt cgcgtcgcgg
      661 ccgaccgggc acacatagtc gcggccggcg agcggctcgc ggatcaggtc ggagttgaac
      721 gccgacagcg ccccgttggg cgtgcgcggc cggacgacga cgccgggcag ccgcagcgct
      781 cggccgtcga tgaagccgcg ccgcgtgtag tcgtcgatca gcacttcgca ggcccgcttg
      841 tgcgcgccgt agctgaggcc gggcaccgcc ggcgtcgcgt cgtcgatgcg cccgggcagc
      901 ggcgcgccga agacggcgat cgagctcgcg tacgtgaagc ggaccagcgg accgccgcgc
      961 tcggcctgct gtcggcagtg ctcgagcaag gcgatggtcg cgtcgaggtt gacgcgcttg
     1021 ccgagcccga aatcgcgctc ggcggcgccg ctgacgacgc cggccaggtg gaagaccgcg
     1081 tcgatcggct cggcgaagag cctcgcctgc agcggcgcgt cgacgatgtc gccttcgatg
     1141 ccgacgatgc gcggatcgcc cgtcgtcgac ccgtcgcccg cgcccgacag atcgaggtcg
     1201 gtgagacaga gcgtcgccgc gttcggcttc gcgacgagat cggcgacgag tctttcgacg
     1261 aggccgcggc cgatgaagcc gccggcgccg gtgacgagcg tgcgcatcaa ccgccgctgc
     1321 cggcgtcggc cggcggcgtc gcggcgccgg cgcggatctc ggtgccgttg agctgctcga
     1381 cccagcgcgc cagctcgctg acgctggcct cggattcgct gtgcgtggcc gccgcgcgcc
     1441 acagctgctg ggccagtgcg cacagcggcg tcaccgagcc agtcgagcgg ccgagctcga
     1501 gggcgatgcc gacgtccttg agcatcagcg cgagcttgaa cgggtcgtcg aaacgacggc
     1561 tgatgacgcg ctggcggatg tgcgtcttcg agatccacga catgccggtc gacacgtcga
     1621 gcacgtcgac catcgccgcc ggatcgagcc cgtagcgctt gccgacgacc aggccttcgc
     1681 tgacggcgag gaagttgacc gcggtgacga ggttgttgag gcacttcatg gcgtggcccg
     1741 cgcccgtgcc gccgacatgg aagacggcct gtcccatgac ttcgagcagc ggccgcacgc
     1801 gctcgacatc ggcagcggcg ccgccgacca tgaagacgag ctcggccgcc tcggcgcccc
     1861 attgcgctcc cgacaccggt gcgtcgacca tcgcgacgcc gcggctggcc agcaatgccg
     1921 ccgagcgctc ggtgagccac ggctcgcacg acgaggtatc gagcagcagc gatcccggtg
     1981 ccatgccatg cagcaggccg tcgtcgccgg tgatcagcga atggacgacc tcgccgttgg
     2041 gcaccatggt gacgacgacg tcgcactcgc gcgcgagcgc ggccggcgaa tcgaaggcgc
     2101 gcgcgccttc gagcgaagcg gcgatgccgg cggcgcggcc gggtgcaagg tcgaagagtc
     2161 gaagcgcgtg gccggcccgc gacaggtggc cggccatcgg accgcccatc acgcccaggc
     2221 cgatgaagcc gacgacggcc cgcggaattg attgacacat aaagggttta cgagatcgac
     2281 gatcggccgc tgatgcaggc aagctacgcg cctcgcgatt gcctgcgcga cgccgcggcc
     2341 tctctgccgc gagccgcaca cgatacgcct ccggtttcca gctcgaacgc catcggaaag
     2401 aacctcgcca tgactgcatc ccgtcgaacc ctcctgctcg gcgctgccgc gatcgccgcc
     2461 gccccgcgcg tcgtcctcgc cgcctcgacg acgacgctga cgatgtcgag ctgggtcggc
     2521 ccgaatcacc tgctgactcg cgacgtgctg cagggctggg ccacctcggt cgagaaggcg
     2581 acgaacggcc gcgtcaagtt cgagatgctc gccaagcatc cgtcgtcgcc gcccggcacc
     2641 ttcgacgccg tgcgcgacga cctcgtcgac gtctcgtacg tcacggccag ctacacgccg
     2701 gcgcgccatc cgctgccgct gctggccgag ctgcccggcg ccggcgccac ggccgagatc
     2761 aactcggtgg cctattcgcg catccactgg aagtacctcg aggcagcgca cgagtacaag
     2821 gaagtgaagc tgctgggcgt cttcacgcac ggccccggcc agatgttcat ggtgaaaaag
     2881 ccgatccacg ccgtcgccga cctggccggc ctgaagatcc gcaccggcgg cggcatcgcc
     2941 gaggccgtgg cccgggcgct cggcgcatcg ccgttcgtca agccggcgcc cgagtcgtac
     3001 gagcttcttt cctcgggcat cgccgacggc accttctttc cttccgagtc gatcaagtcg
     3061 ttcaacctcg acaaggtcgt caagtacgcg accttcttcc ccggcggcct ctactcgtcg
     3121 gcgttcggct tcttcatgaa cctcgacaag tggaacaagc tgccgaagca ggaccaggac
     3181 gcgatcacgt cggtgtcggg cgaggtgctg gcgcggatgg ccggcaaggc ctgggacgct
     3241 gccgacaagg tcggtctggc cgcgatgaag gctgccggcg tgcagatcac ggaagccagc
     3301 ccccagttca tcgccgacga gcatcggctg accgcgccgc tggtcgacaa gtggatcaag
     3361 tcggcagacg ccaagggcat cgacggcgcc aaggtctacg ccgccttcca cgaggagctg
     3421 aagaaggtcg cggcgggtca gtgacgcggc gccaccggtc tcggctgcct gcgaagagaa
     3481 tcgcgccatg tccggcgaag accctcgtcc tgcgctgact cgctcggccc gcatcctcgc
     3541 cgacgagggt cacgaatcgg ggctcgccgg ccagctgacg gcgcgcggcc cgaagccggg
     3601 aacgttctgg accttgccgc tcggcctggc cttcgacgag gccggggagt catcgtggct
     3661 gctcatcgac gacgagctgc gcgtgctcga agggcccggc tcgcgcggcg caagcgccgc
     3721 catcgccgag ccgaatcccg ccacccggtt ccacctgtgg gtctatcggg cacgcgccga
     3781 tgttcattcg atcgtccaca cccacccgcc ggcggcttcg gcgctgtccg cggccgagca
     3841 gccgctcatc gtcgcgcaca tggacgcgac gccgctcttc gacgacatcg ccttcctcgg
     3901 caagtggccg ggcctgccga cggccgatcg cgaaggcgag gtgatcgccg gcggcctcgg
     3961 cgacaagcac gcgatcctgc tcgcccacca cggcctgctc gccgccggcc gctcggtcca
     4021 ggaggcgacg ttcctcgccg tcttcatcga gcggatggcg cgccagcaga tcgacgccgc
     4081 ggcggtcggc ggctcgcggc cggtcgacgc cgacgaggcc aggcgcgccc gcgacttcct
     4141 gcgcaccgac aagatcatga acctcacctt cgacgcctgg gcccgccgcg ccgatcggcg
     4201 ccgcgccggc ccgcagtgaa ccgcgtcgac gtctggctgc agcgcgtcct cggcatcgtc
     4261 gccgcggtcg tgctgttcct gatgatgacg atcacggcga ccgacgtgat cggccgctac
     4321 gtcttcaaca agccgatcaa cggcggcttc gagctcaccg agctgctgct cgcgacgctc
     4381 atctattgcg gcctgccgct ggtctcggcg cgtcgcgagc acatcgtcat cgacaccttc
     4441 gatccgttct tttccgtccg cttcaagcat gcgctcgacg tcatcgccga agtggcgtgc
     4501 gccgtcgcct tcaccggtgc cggcgccctc gtcttcctgc gcgcggtgcg ggtcgccaac
     4561 tacggcgaca cgaccagcgt cctgcacatc ccgctggcgc ccgtggccta cctgatggcg
     4621 gcgatgatcg tcgtcgccgc gctcgtccac ctctggctgg tgttcgcgcc gcggcccgcg
     4681 acggggccgc gcacgacgcc ggaaagcgga gccagcgcgt gaccgagtcg ctcatcgccc
     4741 tggcggcgat gctcgtgctg tcgttcctgc gactgccgat cgccatcgcg atgggcggcg
     4801 tcggctttct cggcctgtgg tggatgcgcg gcctgcaccc ggcgatggca tcggcgacga
     4861 ccgtgctcta cgagtcgggc ttcgaataca gcctcaccgt cgtgccgctc ttcatcctga
     4921 tgggcagctt cgtcacccgc gccggcatgt cgaaggagct ctaccgcgcc gcccacacct
     4981 tcgtcggcca ccgccgcggc ggcctggcga tggccaccgt gctggcctgc gccggcttcg
     5041 gcgcgatctg cggttcgtcg atcgccaccg cggcaacgat gacgcgggtg gcctacccgt
     5101 cgatgcgcga caacggctac tccgaggagc tcgcggtcgg ctcgatcgct gccggcggca
     5161 cgctcggcat cctgatcccg ccctcgacca tcatggtcct ctacggcatc ctcaccgaga
     5221 ccaacatcgg caagctgttc gcggccggcg tgctgccggg cctgatcgag accttcgtgc
     5281 tgtgcctggc cgtgcagtac atgtgctggc gcaatcccgc cgccgggcca cgcgggccac
     5341 gcatcggctg ggccgagcgc ggccgggcgc tgcgcgacgt ctgggccgtc gccgcgctgt
     5401 tcggcgtcgt catgggcggc atctacggcg gcgtgttcac cgccaccgaa ggcgccggcg
     5461 tcggtgcctt cggcgccttc ctcttcgcgc tggcgcgccg ttcgctcgac tgggcgggcc
     5521 tgctcgacgt gctggtcgac agcgcccgca cgacgtcgat gctcttcatc atcctggtcg
     5581 gcgcgctgat cttctcgaac ttcgtcgact acacgagcat gccggcggac ctgaagagct
     5641 tcgtcaccga ccaccacctg catccgctca cggtcgtcgc cgcgatctgc ttcatctaca
     5701 tcgtcctcgg cacggccatg gaggagctct cggtgatcct gctgacgatc ccgatcttct
     5761 ttccgctcgt catcggcctc ggcttcgatc cggtctggtt cggcatcatc atcgtcgtcg
     5821 tcatgcagat cggcctcatc agcccgccgg tggggatgaa catgttcgtc gtcaagagcc
     5881 tgcttcccgg cgtctcgacg cgggcgatct tccgcggcgt gacgccgttc gtgttcgccg
     5941 agatcctggt gctggcgctg ctcgtcgcgg tgccgtggat cagcgtcgtg ctgcccgggc
     6001 tgctgaagct ctaggcttcg gcggcggccg tcggcaggcc gacgatctcg acccagttcg
     6061 gatcggcacc gacatcgacg ctgccgtggc gcgcgaggcg gcccgtcccg gcgtcgatgc
     6121 cgtggacgca gacgcgcccc gacagct
//
LOCUS       DASRWS010000639         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3987731, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000639 DASRWS010000000
VERSION     DASRWS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3987731"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VFF72_12210"
     CDS             complement(<1..133)
                     /locus_tag="VFF72_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HZV93973.1"
                     /translation="MRLDGHDAAAVEVAAARLAEGDVVAFPTETVYGLGARADDDAAV
                     "
     gene            complement(133..1380)
                     /locus_tag="VFF72_12215"
     CDS             complement(133..1380)
                     /locus_tag="VFF72_12215"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZV93974.1"
                     /translation="MLVLASEVTAAPQSTSASRDAVATTFIAPGATLGVIGGGQLGRM
                     FAHAAQRSGYAVAVLDPDEASPAGRVADLHIRAAYLDVEGLARLAEASAAVTTEFENV
                     PAAALQSLAASRPVAPAAATVAICQDRRLEKAAFAGCDVPCAPFAAIECEADLAAVDA
                     ALLPGILKTARLGYDGKGQRRVADRLELSRAFADLGGVACVLEQRLDLAAEVSVIVAR
                     NARGEMVELPVQENLHRDGILAVTEVPAPGIGAEMQAASVALARRVCESIGHVGVLCV
                     ELFVLGDGRLVANEMAPRPHNSGHYSIDACDVSQFDLQVRVLTGAPLVAPRLHSAAVM
                     LNLLGDLWWRESASAAHEPDWRAVLALPGACLHLYGKREARRGRKMGHLTFIAADAAQ
                     ARDRARAAGALLGIAAEALPVSR"
     gene            complement(1475..1939)
                     /gene="purE"
                     /locus_tag="VFF72_12220"
     CDS             complement(1475..1939)
                     /gene="purE"
                     /locus_tag="VFF72_12220"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZV93975.1"
                     /translation="MGSSSDWETLRAATEILAEFGVAHEARVVSAHRMPDDMFAYAEG
                     AAGRGLKAIIAGAGGAAHLPGMLAAKTTVPVLGVPVPSRQMQGLDSLYSIVQMPRGIP
                     VATFAIGVGGAANAALFAVAMLATGDASLRGRLEAFRTRQTEAARAMSAELK"
     gene            2091..3038
                     /locus_tag="VFF72_12225"
     CDS             2091..3038
                     /locus_tag="VFF72_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV93976.1"
                     /translation="MQDITSKNFEIELIEASQQQPVLLDIWAAWCGPCKTLGPILEKL
                     ETSYEGRFKLVKLDADAEPEIAQQLSQMFGVRSIPFCVLFKGGQPVDGFVGALPEGEV
                     KKFLDKHVPSAEEQAAADGLQEAEELLAEGDAETAIDKLQAAVATHPGNDAARYDYLR
                     ALLFAGRIDEARKAFDPVAGNVILDPRLAAAGHWLAAKEMAATARPRQALEAALATNK
                     RDFEARFELGQSLFAAGDFTGAMDELLEIVMRDKAWKDELARKTYVAILEIMTKAVPV
                     PKGEAAPKKGALEVAGKIAATPSDPVVDAYRRKLSMALF"
     gene            complement(3098..4030)
                     /locus_tag="VFF72_12230"
     CDS             complement(3098..4030)
                     /locus_tag="VFF72_12230"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZV93977.1"
                     /translation="MSSSSFSAAAGGADAPLRESRLHSLPLVARGKVRENYAVGSDRL
                     LMVASDRLSAFDVVMGEPIPGKGRVLTQMALFWFGKLAHIVPNHLTGDDPESVVAADE
                     REQVRGRSMLVKRLEPLPIEAVVRGYLAGSAWKEYREGGSVCGVALPPGLKNASKLPA
                     PIFTPATKAEAGEHDENIDFDTAADRIGHDLAARMRDTAIRLYSEAAQMALARGIVIA
                     DTKFEFGLDEDGAMVLMDEVLTPDSSRFWPADAWAEGANPPSFDKQFVRDWLESVRID
                     GRPWPKRPPAPTLPADVIEKTAATYRAVADILMR"
     gene            complement(4156..>4463)
                     /locus_tag="VFF72_12235"
     CDS             complement(4156..>4463)
                     /locus_tag="VFF72_12235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZV93978.1"
                     /translation="SSPMYIVTVVFSVREPHVADFHAAIVDNAKVSRGREPGCRQFDV
                     CVAADDPSTIFLYEVYADRAAFDAHLASSHFKAFDASAASWIRDKVVCTYERIDPVA"
BASE COUNT          698 a         1564 c         1573 g          628 t
ORIGIN      
        1 ccacggccgc gtcgtcgtcg gccctggcgc ccaggccgta gaccgtttcc gtcgggaacg
       61 ccaccacgtc gccttcggcg aggcgagcag cggccacctc gaccgcagcg gcgtcgtgac
      121 cgtcgaggcg catcagcgcg aaaccggcaa tgcctcggcg gcgatgccga gaagcgcgcc
      181 ggcggcgcgg gcccgatcgc gggcctgcgc cgcgtcggcg gcgatgaacg tgaggtgacc
      241 catcttgcgg ccgcgccgcg cctcgcgctt gccgtagaga tgcaggcagg caccggggag
      301 cgccagcacg gcgcgccagt cgggctcgtg cgccgcgctg gcgctctcgc gccaccacag
      361 gtcgcccagc aggttgagca tcaccgccgc cgagtgcagg cgcggcgcga cgagcggcgc
      421 accggtgagg acgcgcacct gcagatcgaa ctgcgacacg tcgcaggcat cgatgctgta
      481 gtggccggag ttgtgcgggc gcggcgccat ttcgttggcg acgaggcgac cgtcgccgag
      541 cacgaacagc tcgacgcaga gcacgccgac atggccgatc gactcgcaga cgcgcctggc
      601 gagcgcgacc gacgcggcct gcatttcggc gccgatgccg ggcgccggca cctcggtcac
      661 ggcgaggatg ccgtcgcggt gcaggttttc ctgcaccggc agctcgacca tctcgcctcg
      721 cgcgttgcgc gcgacgatga cgctcacctc ggccgcgagg tccagccgct gttcgagcac
      781 gcaggcgacg ccgccgagat cggcgaaggc ccgcgagagc tcgaggcgat cggcgacccg
      841 gcgctggccc ttgccgtcgt agccgaggcg cgcggtcttg aggatgccgg gcagcagcgc
      901 cgcgtcgacc gcggcgagat cggcttcgca ttcgatcgcg gcaaaaggtg cacagggcac
      961 gtcgcacccg gcaaaggcgg ctttctcgag ccgtcgatcc tggcagatcg ccaccgtcgc
     1021 cgcggccggg gcgacgggcc ggctcgccgc cagcgattgc agcgccgcag ccgggacgtt
     1081 ctcgaattcg gtcgtgaccg ccgcgcttgc ttcggccagc cgggcgagac cttcgacgtc
     1141 gaggtaggcg gcgcggatat gcaggtcggc gacgcggccg gccgggctcg cctcgtcggg
     1201 gtcgagcacc gcgacggcat agccgctgcg ctgcgcggcg tgcgcgaaca tccggccgag
     1261 ctggccgccg ccgatcaccc cgagcgtcgc gccgggagcg atgaacgtcg tcgcgaccgc
     1321 gtcccggctc gccgacgtcg actgcggggc tgcggtcact tcgctggcga ggacaagcat
     1381 ggcggccggg ccggggccat cgatccgcct tcgccgagcc atcgatggtc gcccacccga
     1441 ggcgcggcga ccgaccgcga ggcgcgaccg ggcctcactt caactcggcc gacatcgccc
     1501 gcgccgcctc ggtctggcgg gtgcggaacg cttcgagccg gccgcgcagc gatgcatcgc
     1561 cggtggccag catggcgacg gcgaagagcg cggcgttggc ggcgccgccg acaccgatgg
     1621 caaaggtcgc caccggaatg ccgcgcggca tctgcacgat cgaatacagc gaatcgagac
     1681 cctgcatctg ccggctcggc accggcacgc cgagcaccgg cacggtcgtc ttggcggcga
     1741 gcatgccggg caggtgcgcg gcgccgcccg cgccggcgat gatcgccttc aggccgcggc
     1801 cggcggcgcc ttcggcatag gcgaacatgt cgtcgggcat gcggtgcgcc gagacgaccc
     1861 gcgcttcgtg ggcgacgccg aactcggcga gaatctcggt ggcagcgcgc agggtttccc
     1921 agtcgctgct ggaacccatg acgacgccga tgaccgcggg tgcgctgctc actgtcggga
     1981 ctccgtcgct cgggtacgct tcgagccttg aattgtaggc ggcgaggccc gaactcacgc
     2041 cttccacccc atgcgccgcc accgcggtat cggcgccgca agagcatccc atgcaggaca
     2101 tcaccagcaa gaacttcgaa atcgagctga tcgaggcatc gcagcagcaa ccggtcctgc
     2161 tcgacatctg ggccgcctgg tgcggcccgt gcaagacgct ggggccgatt ctcgagaagc
     2221 tcgagacctc ctacgagggt cgcttcaagc tggtcaagct cgacgccgac gccgagcccg
     2281 agatcgcgca gcagctgtcg cagatgttcg gcgtgcgcag cattccgttc tgcgtgctct
     2341 tcaagggcgg ccagccggtc gacggcttcg tcggcgcgct gcccgagggc gaggtgaaga
     2401 agttcctcga caagcacgtg ccgagcgccg aggaacaggc cgccgccgac ggcttgcagg
     2461 aagccgagga gctgctcgcc gagggcgatg ccgaaaccgc gatcgacaag ctgcaggccg
     2521 ccgtcgccac ccatccgggc aacgacgcgg cgcgctatga ctatctgcgc gccctgctct
     2581 tcgccggtcg catcgatgaa gcgcgcaagg ccttcgatcc cgtcgccggc aacgtcatcc
     2641 tcgatccgcg gcttgccgcg gccggccact ggctggcggc caaggagatg gccgccacgg
     2701 cgcggccgcg ccaggcgctg gaagcggcgc tggcgacgaa caagcgcgat ttcgaggctc
     2761 gcttcgagct cggccagagc ctcttcgcgg ccggcgactt caccggtgcg atggatgagc
     2821 tgctcgagat cgtgatgcgc gacaaggcct ggaaggacga gctcgcgcgc aagacctacg
     2881 tcgccatcct cgagatcatg accaaggccg tgcccgttcc caaaggcgag gccgcgccga
     2941 agaaaggcgc gctcgaggtc gccggcaaga tcgcggcgac accgagcgat ccggtcgtcg
     3001 atgcgtatcg gcgcaagctg agcatggcgc tgttctaggc gcttcgccgg gggcgcgcaa
     3061 gcgccacccg gtccgagtgg cgcttgcgcg ccccgtgtca gcgcatcagg atgtcagcca
     3121 ccgcgcggta ggtcgccgcc gtcttctcga tcacgtcggc cggcagcgtc ggcgccggcg
     3181 ggcgcttcgg ccacggccga ccgtcgatgc gcacgctttc cagccagtcg cggacgaact
     3241 gcttgtcgaa gctcggcgga ttggcgcctt cggcccaggc atcggccggc cagaagcgcg
     3301 acgagtcggg ggtcagcact tcgtccatca gcaccatggc gccgtcttcg tcgaggccga
     3361 actcgaactt cgtgtcggca atgacgatgc cgcgcgccag cgccatctgc gccgcctcgc
     3421 tgtagagacg gatcgcggtg tcgcgcatcc gcgccgcgag atcgtggccg atgcggtcgg
     3481 cggcggtatc gaaatcgatg ttctcgtcgt gctcgccggc ctcggccttg gtcgccggcg
     3541 tgaagatcgg cgccggcagc ttcgaggcat tcttcaggcc cggcggcagg gccacgccgc
     3601 agacgctgcc gccctcgcga tattccttcc aggcgctgcc ggcgagatag ccgcgcacta
     3661 ctgcctcgat cggcagcggc tcgaggcgct tgacgagcat cgagcggcct cgcacctgct
     3721 cgcgctcgtc ggcagcgacg acgctctcgg gatcgtcgcc ggtcaggtgg ttgggcacga
     3781 tgtgggcgag cttgccgaac cagaagagcg ccatctgcgt cagcacgcgg cccttgcccg
     3841 gaatcggctc gcccatcacg acgtcgaagg cgctcaggcg atcactcgcc accatcagca
     3901 acctgtcgct gccgacggcg tagttctcgc ggaccttgcc acgcgcgacc agcggcagcg
     3961 aatgcaggcg gctttcgcgg agcggcgcgt cggcgccgcc ggcggcggcc gagaaggaag
     4021 aactgctcat ggatcggcgc gtctgcgctt tgcgcgaggc agtgaaacct gggtcatcct
     4081 cgtatcgtac ggccgacttc gccacccgcg gccccccatg ccgtcagcgc gatgcacggc
     4141 gtacatgtcc gtgaatcagg cgacggggtc gattcgctca taggtgcaaa ccaccttgtc
     4201 gcgaatccag gaagcggccg acgcatcgaa tgccttgaag tggctgctcg cgagatgagc
     4261 gtcgaaggcc gcgcgatccg catagacctc gtagaggaag atcgtcgacg gatcatcggc
     4321 ggcaacgcac acatcgaact gccgacagcc gggctcgcgc ccgcgcgata ccttcgcgtt
     4381 gtcgacgatg gcggcgtgga aatcggccac atgcggctcg cggacggaaa agacgacggt
     4441 gacgatgtac atcggactgc tca
//
LOCUS       DASRWS010000640         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1124232, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000640 DASRWS010000000
VERSION     DASRWS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124232"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFF72_12240"
     CDS             <1..407
                     /locus_tag="VFF72_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93979.1"
                     /translation="IVAANLLDKGARKVTDRIPCYALFTDPPLGRVGMGEAEVRARGK
                     PALVATMPMTRVGRARERGETQGFMKVFVDAESERILGAAMLCIEGDEVVHSILDVMA
                     AGASYKVIRDAVHIHPTVSELIPTMLESLQPL"
     gene            488..1357
                     /locus_tag="VFF72_12245"
     CDS             488..1357
                     /locus_tag="VFF72_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HZV93980.1"
                     /translation="MRNIDEHNITDAVLARFESCNDPRLKEILGSLVRHLHAFVRETK
                     LSEAEWLRGIEFLTATGHQCDDKRQEFILLSDTLGVSMLMVAQNHRLVDGATEATVFG
                     PFHVESAPAFDDGADLAAGAPGEPLFVEGTIRGRDGEKVAGATIDVWQADQEGLYDVQ
                     YKDLDGHRARGILRSDTEGRFRFRSILPVAYPVPTDGPVGQMLVASGRHPWRPAHVHF
                     RIEAPGYETLTTHVFRSGDPYLDSDVVFGVRSSLVSDFVAHARGTGPHAGDIDLPYHT
                     LDFDFVVQRRAAA"
     gene            1455..1979
                     /locus_tag="VFF72_12250"
     CDS             1455..1979
                     /locus_tag="VFF72_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZV93981.1"
                     /translation="MAIYRIGDASPQVSASAYVAAEATLIGHVVLGENTSFWPGATAR
                     GDNETITIGEGSNVQEGAVLHADPGFPLELKPGVTVGHQAMLHGCTIGEGSLVGIQAV
                     VLNGAVIGRNCLVGAGAIVTEGKTFPDRMLILGAPARAVRELNDAEVAGVVAAAQHYV
                     ERGAHFRSALVRIG"
BASE COUNT          325 a          756 c          656 g          282 t
ORIGIN      
        1 agatcgtcgc cgccaacctg ctcgacaagg gcgccaggaa agtcaccgac cgcatcccct
       61 gctacgcgct cttcaccgac ccgccgctcg gccgcgtcgg catgggcgag gccgaggtgc
      121 gcgcccgcgg caagcccgca ctcgtcgcga cgatgccgat gacgcgggtc ggccgggccc
      181 gcgagcgcgg cgagacgcaa ggcttcatga aggtcttcgt cgacgccgaa agcgagcgga
      241 tcctcggcgc ggcgatgctc tgcatcgaag gcgacgaagt cgtccactcg atcctcgacg
      301 tcatggccgc gggggcgtcg tacaaggtca tccgcgacgc ggtgcacatc catcccaccg
      361 tgagcgagct gatcccgacg atgctggaga gcctccagcc gctctgaaaa ccaggcgacg
      421 acgcgccgca gccgacgcgt cggcgcgtac ccgtgctctt gcacgctccc gagctctccg
      481 acctgccatg cgaaacatcg acgaacacaa catcacggac gcggtcctcg cccgcttcga
      541 aagctgcaac gacccgcgcc tgaaggagat cctgggctcg ctggtacggc acctgcacgc
      601 cttcgttcgc gagacgaagc tgagcgaggc cgaatggctg cgcggcatcg agttcctgac
      661 cgccaccggc caccagtgcg acgacaagcg gcaggagttc atcctcctgt ccgacacgct
      721 cggcgtctcg atgctgatgg tggcgcagaa ccatcgactg gtcgacggcg ccaccgaggc
      781 gaccgtgttc gggccgttcc acgtcgagtc ggcgccggcc ttcgacgatg gtgccgacct
      841 cgccgccggc gcgcccggcg agccgctatt cgtcgaaggc acgatccgcg gccgcgacgg
      901 cgagaaagtc gccggagcga cgatcgatgt ctggcaggcc gaccaggaag gcctctacga
      961 cgtccagtac aaggacctcg acggccaccg cgcccgcggc atcctgcgca gcgacaccga
     1021 ggggcggttc cgctttcgca gcatcctgcc ggtcgcctat ccggtgccga ccgacgggcc
     1081 ggtcgggcag atgctggtgg caagcggccg ccacccgtgg cggccggcgc acgttcattt
     1141 ccgcatcgag gcgccgggct acgagacgct gaccacccac gtctttcgct ccggcgaccc
     1201 gtacctcgac agcgatgtcg tgttcggcgt ccgctcgtcg ctggtcagcg atttcgtcgc
     1261 ccacgcccgc ggtaccggcc cgcacgccgg cgacatcgac ctgccctatc acacgctcga
     1321 cttcgacttc gtggtgcagc ggcgcgccgc ggcctgaagc gcctgcacga tactttcggt
     1381 ccgaacagca acacggcgcc gcgaatcgcc agggctccct ctggcccggc ccggctcccg
     1441 ccccgacccg caccatggcc atctatcgca tcggcgacgc ctcgccccag gtttccgcat
     1501 ccgcctacgt cgccgccgag gcgacgctga tcggccacgt cgtcctcggc gaaaacacca
     1561 gcttctggcc cggcgccacg gcgcgcggcg acaacgagac gatcaccatc ggcgaaggca
     1621 gcaacgtcca ggaaggcgcg gtgctgcacg ccgatccggg ctttccgctc gagctgaagc
     1681 ccggcgtgac ggtgggccac caggcgatgc tgcacggctg cacgatcggc gaaggttcgc
     1741 tcgtcggcat ccaggccgtc gtcctcaacg gcgccgtgat cggcaggaac tgcctcgtcg
     1801 gcgccggcgc catcgtcacc gaaggcaaga cattccccga ccgcatgctg atcctcggcg
     1861 cgccggcccg ggcggtacgc gagctgaacg acgccgaagt ggccggcgtc gtcgccgcgg
     1921 ctcagcacta cgtcgagcgc ggcgcccatt tccgcagcgc gctcgtacgc atcggctgag
     1981 gcgcggacgg cggcgttcgg ccgcgcacca gtcgacgcc
//
LOCUS       DASRWS010000641         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2541577, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000641 DASRWS010000000
VERSION     DASRWS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541577"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1142)
                     /locus_tag="VFF72_12255"
     CDS             complement(153..1142)
                     /locus_tag="VFF72_12255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV93982.1"
                     /translation="MKTRQLGARGPSVSAIGLGCMGMSEFYSNRDDGESLATLHRALD
                     LGVGFLDTSDIYGPHTNEELVGRAIKGRRSEVFLATKFGILRDPANPQLRGFDGSPAY
                     VRRAAEASLKRLGIETIDLYYQHRVDPKTPIEETVGAMSDLVRAGKVRFLGLSEASTA
                     TLERACKIHPITALQSEYSLWTRDPEEGVLAACRRLGVGFVPYSPLGRGFLTGAITRP
                     EDLAEDDYRRHSPRFQGDNFAKNLELARKVQELAAGRGCTAGQLALAWVLAQGDDVVP
                     IPGTKRRRYLEENVAAVDIVLSPGELAELDAVFPPGAAAGLRYPENMMKALNG"
     gene            2310..2444
                     /locus_tag="VFF72_12260"
     CDS             2310..2444
                     /locus_tag="VFF72_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV93983.1"
                     /translation="MQQGLASGANEALTFGANEFACDLFEREMESLATAPRAAPSPRD
                     "
     gene            complement(2441..2806)
                     /locus_tag="VFF72_12265"
     CDS             complement(2441..2806)
                     /locus_tag="VFF72_12265"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZV93984.1"
                     /translation="MATYGKKASEKVEKTMRERKAGTLKSGGSGKKVTSRKQAIAIGL
                     SEARAAGGKVPAKKSAAKTTSAKKSTPRKSAARKTTAKKATSGRATARKATAGKSPAR
                     KSPSRKTPGKKTPAKKTMR"
BASE COUNT          397 a         1072 c         1030 g          549 t
ORIGIN      
        1 tggggggcaa gccggtgggt cgcaatcgct tctacggcgc caacgatccg cgccgcaaca
       61 ccgggctcgc tctgggctat tgagcgacga gactgagcga cgggaccggg cgccgcggtt
      121 cgagcgccgg cgcgacgcgc cagcgagggc gatcagccgt tcagcgcctt catcatgttc
      181 tcggggtagc gaaggcccgc tgccgcaccg ggcggaaaca cggcgtcgag ctcggcgagc
      241 tcgccgggcg agagcacgat gtcgacggcg gcgacgtttt cctcgagata gcgacgccgc
      301 ttcgtgcccg gaatggggac gacgtcgtcg ccttgcgcca gcacccaggc cagggcgagc
      361 tggccggcgg tgcagccgcg gcccgcggcc agctcctgca ccttcctggc cagctcgagg
      421 ttcttcgcga agttgtcgcc ctggaaccgc gggctgtgcc ggcggtagtc gtcctcggcc
      481 aggtcttccg gcctagtgat cgcgccggtg agaaaaccgc ggccgagcgg gctgtagggg
      541 acgaagccga cgcccaggcg ccggcaggcg gcgaggacgc cttcttcggg atcgcgcgtc
      601 cacagcgaat attcgctctg cagggcggtg atcgggtgga tcttgcaggc acgctcgagg
      661 gtcgccgtcg acgcttccga aagaccgagg aagcggacct tgccggcacg gaccaggtcg
      721 ctcatcgccc ccaccgtctc ctcgatcggc gtcttcggat cgacgcggtg ctggtagtag
      781 agatcgatcg tctcgatgcc gaggcgcttc aggctcgcct cggcggcgcg ccgcacgtag
      841 gccgggctgc cgtcgaagcc gcgcagctgc gggttggccg gatcgcgaag gatgccgaac
      901 ttggtcgcca ggaagacttc gctgcgccgc cccttgatgg cccggccgac cagctcctcg
      961 ttggtgtgcg gcccgtagat gtcggaggtg tcgagaaagc cgacgccgag atcgagggcg
     1021 cgatgcaggg tggcgagcga ttcgccgtcg tcgcggttcg agtagaactc gctcatgccc
     1081 atgcagccga ggccgatggc ggaaacgctc gggcctcggg cgccgagctg gcgggtcttc
     1141 atggttcgtt tcctcggaag ggtcgggctt tcgacgatag cgcgaaggac gaaaccgcag
     1201 cagcatgacg accatcgttc gctcggagcg ccgcgcgcgg cgcggcgcag catcgttcct
     1261 cgatcgggcc ctatctcgca catgacgacc gacgatgcct cggccctttg gcgacgtgcc
     1321 cgcgaggcga tctccgggac gcggccgcag cgcggagcgc cggcgaggag cccggtggcc
     1381 cgctacctcg atcccgcacg ccacgcacgc gaggtcgcgg tgctgcgggc ctggccgcat
     1441 gcggtcgcgc cggtcgcgca gctcgccgcg ccgggcgact ggctgggcac ttccgtgctc
     1501 ggcgtgcccc ttcttctcgt tcgcgccgac gacggcgtgt tgcgggcgtt catcggcgtc
     1561 tgccggcatc gcggcgccgt cgtcgtcggc gacggcgatt gcgggcacgc gcggagccgt
     1621 tttgtgtgcc cgtatcacag ctggacgtac gacagccacg gccacctcgt cggccggccg
     1681 cacgatgccg actttcctca cgtggcccgt gccgacgcct cgctcgtcga gctgccggtg
     1741 gccgagcgct gcggcctcgt ctgggtggtg ccgtcgccga acgcggactt cgactgggac
     1801 gactatttcg gcagcctcgc tctcgagctc ggcgggctcg gctacgactc tctcgccgcg
     1861 agcccgcagc agcgccgtgt cgtgcatgcc tcgaactgga agctcgtcct cgacgccaac
     1921 ctcgagagct atcacttcca gtacgcgcat cgggcgacga tcgcccatct cttccacgac
     1981 aacctggtgc agcaggagtc gttcggccgg caccagcgga tcgtcctggc caagcgcagc
     2041 ctcgtcgagg ccgatcccgc cgacacgtcg tggacgctgc tcggccggca cagcaacatc
     2101 atctacttct tcttccccgc gaccttcctc ctctgggaag gcgatcacgt caacggcttc
     2161 aacgtgacgc cgctgtcgcc ggatcgttcg accgctgcca gctggatgct cgtcccgccg
     2221 gcgcaggccc atcgccccgc cttgcactgg cagcgcaact ggcgcatctt ctgggacgcg
     2281 atcgacgaag attcgccctc gccgcatcga tgcagcaggg cctggcgagc ggcgccaacg
     2341 aggccctcac cttcggtgcc aacgagttcg cgtgcgatct cttcgagcgc gagatggaat
     2401 cgctcgcgac ggcgccgcgc gccgcgccgt cgcctcgaga ctagcgcatc gtcttcttcg
     2461 ccggggtctt cttccccggg gtcttgcgcg acgggctctt gcgcgccggg gacttgccgg
     2521 cggttgcctt cctggcggtc gctcgtccgg acgtcgcctt cttggcggtc gtcttcctgg
     2581 ccgcgctctt cctcggcgtg ctcttctttg cgcttgtcgt tttcgctgcc gacttcttcg
     2641 ccggcacctt gccgccggcg gcgcgcgcct cggacaggcc gatcgcgatc gcctgcttgc
     2701 ggctcgtcac cttcttgccg gaaccgccgc tcttcagcgt tcccgccttg cgctcgcgca
     2761 tcgtcttttc gaccttttcg gaagccttct tgccatacgt tgccatgtcg gatcctcgtc
     2821 tcgtgagtga acgtggtacg acgccccggc aggagtcgtg cctgcggtct tgacggattc
     2881 ttgatgcggg cgggcagcct ctctacgccg aattgaccac cgcagtccta gcgttcgtcc
     2941 tcgaccaagg aggatcgaac ccgtggactt cgtcttcgtt gccctgatcg cgtctttcgt
     3001 cgccgccatc gtcggcctcg tggctgcctg cgacgccctc ggagcgcg
//
LOCUS       DASRWS010000642         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1125919, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000642 DASRWS010000000
VERSION     DASRWS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125919"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFF72_12270"
     CDS             <1..501
                     /locus_tag="VFF72_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZV93985.1"
                     /translation="AFAGPTYESKQAIVVKSDSKTFGNSIAGFAALNGHTVCVVSGSV
                     SNTLLLKDAPQAKIMALTANPDCAKAVRQGRVEAFATDDAILIGFVKETPTELRFVDN
                     AGGNEEPYGIGINKDSLDLCRWVNERLLNMSRDGYLEKSFAATIGSVTPRKLAPLNEA
                     AMTYCR"
     gene            578..1219
                     /locus_tag="VFF72_12275"
     CDS             578..1219
                     /locus_tag="VFF72_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV93986.1"
                     /translation="MEFWAQLMPAFRAGLWESLQLALIAAVLAFPLGCFVGVGRIMPI
                     AIVRGLCDLYINLVRSTPIPVIFIFFFFALPDVGLRFSAFGCAAAGLALYMASYVAEA
                     VRSGVNAVPSGYIEAARALGLSERTVIGGILLPQAIKAVIPTLGNLSVDLIKNTSIAY
                     TISVVELTGTAVQQVTMLARPIPIFVLAAVGYALMTFPTGAAFRALEHKFKYR"
     gene            1229..2032
                     /locus_tag="VFF72_12280"
     CDS             1229..2032
                     /locus_tag="VFF72_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZV93987.1"
                     /translation="MDYRLLAKDLSPRAEARLRAVGALVALLCVLALALVAYGLHRTG
                     ELEARRWQIFAHGATWKFLAIGLWNTVRVALADLLFAMALGGLVAMAQTSRYKFLKLA
                     GGAYAEFFRAIPTLLMILFCFFGLPLLGLDVTPFWAIVIGSGLYNAAVLSNIFKSGIQ
                     TIASGQSEAGYSLGLARKHVMVGIVWPQALRRMRPSIVAQCVVLFKDSSLGFVIGYEE
                     VLRRSQEIGTFTSSSLQPYFVGGCIYIVVIYLLRRLSEQGGRRTDEFAA"
     gene            complement(2170..3054)
                     /locus_tag="VFF72_12285"
     CDS             complement(2170..3054)
                     /locus_tag="VFF72_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZV93988.1"
                     /translation="MRELSLDQVRTLVTIVELGSFAAAARALHLAAPTVSLHVAELES
                     RLGAALLLRQRQRVVPTGVGQSLVDRGRRLLSDVDAALDDVARQVEGRAGRVRLGAST
                     GALAWLLPQALETIARDHPGIDVQVAVLTSQEALSRLQEGTLDIGLVALPQAAIKGLQ
                     IVPWRRDPVMAFVPGAWTVPRAITPAWLAARPLILNNASTRLHRLTTEWFAAAGEQPQ
                     ARIELNFNDAIKSLVAAGYGAALLPREGGAAALDARIVAAPLTPRLWRALGLACRAGP
                     PEPATRYMLDALAAMRQR"
     gene            3117..>4044
                     /gene="argC"
                     /locus_tag="VFF72_12290"
     CDS             3117..>4044
                     /gene="argC"
                     /locus_tag="VFF72_12290"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZV93989.1"
                     /translation="MQPSPFSAGNIQMGASVFIDGDQGTTGLEILERLSRRHDIDLVT
                     LPDGRRKDAAARAMAIDDCDVAVLCLPDEASRQAVAGIRNPRVRVIDASSAYRTDAEW
                     VYGFPEMSARQPALIARALRVSNPGCYPTGAVALLRPLVEAGCLRSDAPVSIHAVSGY
                     SGRGREGIARHEGPGAATALSFQTYGLDLQHKHVPEIRLHSGLQGRPLFVPSYGNFRQ
                     GIALTIPLHASMLHWPMTADSLREVLARHYRDQRHVHVLDAVETAATARLDPQALNGT
                     DDLQLAVFGNDAHEQILLSAVFDNLGKGACGAA"
BASE COUNT          638 a         1424 c         1311 g          671 t
ORIGIN      
        1 gccttcgccg ggccgacgta cgaatccaag caggccatcg tcgtcaagtc cgacagcaag
       61 accttcggca actccattgc cggcttcgcg gccctgaacg gacacacggt ctgcgtcgtc
      121 agcggttcgg tgtcgaacac gctgctgctg aaggacgcgc cgcaggcgaa gatcatggcg
      181 ctgactgcca acccggactg cgccaaggcg gtacgccagg ggcgcgtcga ggccttcgcc
      241 accgacgacg ccatattgat cggcttcgtc aaggagacgc ccacggagct gcgcttcgtc
      301 gacaacgccg gcggcaacga agagccctac ggcatcggca tcaacaagga ttcgctcgac
      361 ctttgccgct gggtcaacga gcgcctcctg aacatgtcca gggacggcta tctcgagaag
      421 agcttcgccg ccaccatcgg cagcgtgacc ccgcgcaagc tcgcaccgct caacgaagcc
      481 gccatgacct actgcaggta gccgtcgcgc gttcgccgcg cctgcgcggc ggcgcacacg
      541 gcaggacggc cgcaacggcg cgaatcggaa gctgtcgatg gagttctggg cgcagctcat
      601 gccggccttc agggcgggcc tttgggaaag cctgcagctg gcgctgatcg ccgccgtgct
      661 ggcgttcccg ctcggctgct tcgtcggcgt gggccggatc atgcccatcg ccatcgtgcg
      721 cggcctttgc gacctgtaca tcaacctggt gcgcagcacg ccgatcccgg tgatcttcat
      781 cttcttcttc ttcgccctgc ccgacgtggg cctgcgcttc tcggccttcg gttgcgcagc
      841 cgccgggctg gccttgtaca tggcgtcgta cgtcgccgaa gcggtgcgct cgggcgtcaa
      901 cgcggtgccg tcgggctaca tcgaggcggc gcgcgcgctg gggctttccg agcgaaccgt
      961 catcggcggc atcctgctgc cacaggccat caaggcggtg attcccaccc tgggcaacct
     1021 aagcgtcgac ctgatcaaga acacctccat cgcctacacc atctcggtcg tcgagctgac
     1081 cggcaccgca gtgcagcagg tcaccatgct ggccaggccg attcccatct tcgtcctggc
     1141 cgccgtgggc tacgccctga tgacgtttcc caccggggca gcgtttcgcg ccctcgagca
     1201 caagttcaaa taccggtagc gccgcccctt ggactaccgc ctgctggcca aggacctgag
     1261 cccacgcgcc gaagcgcgtc ttcgcgccgt cggcgcgctg gtggccctcc tttgcgtgct
     1321 cgcgctggct ctggtggcct acgggctcca tcgcacggga gaactggagg cgaggcgctg
     1381 gcagatcttc gcgcatgggg ccacctggaa attcctcgcc atcggcttgt ggaacacggt
     1441 gcgggtggcg ctggccgacc tgctgttcgc catggcgctc ggcggcctgg tcgcgatggc
     1501 gcaaaccagc cgctacaagt tcctgaagct ggccggaggc gcctacgccg agttcttccg
     1561 cgccatcccg acgctgctca tgatcctgtt ctgcttcttc ggcctgcccc tcctcgggct
     1621 cgacgtgacg ccgttctggg ccatcgtgat cggcagcggc ctctacaacg cggcggtgct
     1681 cagcaacatc ttcaagagcg gcatccagac catcgccagc gggcaatcag aggccggata
     1741 ttcgctcggc ctggcgcgca agcacgtgat ggtgggcatc gtctggccgc aagccctgcg
     1801 ccgcatgcgg ccttccatcg tcgcgcagtg cgtcgtcctc ttcaaggaca gctcgctcgg
     1861 cttcgtgatc ggctacgagg aagtcctgcg ccgctcgcag gagatcggca ccttcacctc
     1921 cagctcgctg caaccctact tcgtcggcgg ctgcatctac atcgtcgtca tctacctgct
     1981 gcgccgtctg tccgaacaag gcggtcgtcg aaccgatgaa ttcgcggcct gacgcgaggg
     2041 cgggccgcgc gtaacgcaac cgacggcttc gggagcgcgc cttcctgaca agccgcggga
     2101 cttcggcaac gaatgctcgg cagcgccttg ttccgatcgc gacgagtcgg gcagcagtcg
     2161 ccgccgctct caacgctgcc gcatcgcagc cagcgcgtcg agcatgtagc gcgtggccgg
     2221 ctccggcggc cccgcccggc aggccagacc cagcgcgcgc caaagtcgcg gcgtgagtgg
     2281 cgcggccacg atgcgtgcat cgagcgcagc cgcgccccct tcgcgcggga gcagcgcggc
     2341 cccgtagccg gctgccacca ggctcttgat ggcgtcattg aagttcagct cgattcgcgc
     2401 ctgtggctgt tcgccggcgg cggcgaacca ctccgtcgtc aggcggtgca accgggtgct
     2461 ggcgttgttg aggatcagcg gacgagccgc gagccaggcc ggtgtgatcg cgcgcggaac
     2521 ggtccacgcg cccggcacga acgccatcac cgggtcgcgg cgccagggca cgatctgcaa
     2581 gcccttgatg gctgcctgcg gcagcgccac caggccgatg tcgagggtac cttcctgcaa
     2641 gcgcgacaag gcctcctgcg acgtgagcac ggcgacctga acatcgattc cggggtggtc
     2701 gcgcgcgatg gtctcgagcg cctgtggcag cagccaggcc aacgcaccgg tggacgcgcc
     2761 cagtcgcacc cgaccggcac ggccctcgac ctggcgcgcc acgtcgtcga gcgccgcgtc
     2821 gacatccgag agcaggcggc ggccgcgatc gacgagcgac tgtccgaccc ccgtaggcac
     2881 cacgcgctgg cgctggcgaa gaagcagcgc cgcgcccagg cgtgactcca gctcggccac
     2941 atgcaggctg acggtcgggg cggccagatg caatgcgcgc gccgcggctg cgaacgaccc
     3001 gagctcgacg atcgtcacca gcgtgcgcac ctgatcgagg ctgagctctc tcatcgtcgt
     3061 tcagtacaaa tgaatcttgc attcatgaca ttcaacttta ccaatcggtc ggcgagatgc
     3121 aaccttcgcc cttctctgcg gggaacatcc aaatgggcgc gagcgttttc atcgacggcg
     3181 atcagggcac caccggcctc gagatcctgg agcgactctc gcgacgccac gacatcgatc
     3241 tcgtcaccct gcccgatggc cggcgcaagg acgccgccgc gcgcgcgatg gccatcgatg
     3301 actgcgacgt ggcggtgctg tgtctgcccg acgaggcgtc acgacaggcc gtggccggca
     3361 tccgcaatcc gcgcgtgcgt gtcatcgacg cctcgtcggc gtatcgcacc gatgcggagt
     3421 gggtgtacgg ctttcccgag atgtcggcac ggcagcccgc gctgattgcc cgggcgctgc
     3481 gggtgagcaa tccgggttgt tacccgaccg gtgccgtcgc cctgctgcga cccctggtcg
     3541 aagcagggtg cctgcgcagc gacgcgccag tgagcatcca tgccgtgtcc ggctactccg
     3601 gacgcggacg cgagggcatt gcaaggcacg aaggcccggg cgcggcgacg gccttgtcct
     3661 tccagaccta tggccttgat ctccagcaca agcacgtgcc ggaaatccgg ctgcattcag
     3721 ggcttcaggg ccgaccgttg ttcgtgccgt cgtacggcaa cttccggcaa ggcatcgcgc
     3781 tgacgatccc gctgcacgcg tcgatgctgc actggccgat gacagccgac tcgctgcgcg
     3841 aggtgcttgc acgccactat cgcgatcaac gccatgtgca tgtgctcgat gccgtcgaga
     3901 ccgccgcgac ggcgcgactg gacccgcagg cgctcaacgg caccgacgat ctgcagttgg
     3961 ccgtcttcgg caacgacgcg catgagcaga ttctgctgag cgccgtcttc gacaacctcg
     4021 gcaagggcgc ctgcggcgcc gccg
//
LOCUS       DASRWS010000643         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3127239, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000643 DASRWS010000000
VERSION     DASRWS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3127239"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFF72_12295"
     CDS             <1..247
                     /locus_tag="VFF72_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HZV93990.1"
                     /translation="KIEKIGGDKVKQVVVVRSGIDSDTARKLQTAVKQSKLKVQASIQ
                     GDTVRISGGKRDDLQAAIALVRKELPDQPLSYINFRD"
     gene            249..896
                     /locus_tag="VFF72_12300"
     CDS             249..896
                     /locus_tag="VFF72_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HZV93991.1"
                     /translation="MRTPFMATVFALALAAAASAMAQSVSMGGSLGSHALLVIDGKAR
                     NVAVGSTVDGVRLVSVNGNEAVVEVKGQRVTVMLGGAPANLGGAASEGSGQKIVITAG
                     SGGHFFTTGTINGRSVNFVVDTGATTIALSEAEADRIGLDYKHGRISYSSTANGVVVA
                     HRVSLSSVRIGDVQVYNVAATVLPTPLPIVLLGNSFLERFQMRRENDLLTLDKRY"
     gene            1079..2284
                     /locus_tag="VFF72_12305"
     CDS             1079..2284
                     /locus_tag="VFF72_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV93992.1"
                     /translation="MDLTFTPEEQAFREQVRAWVKDNLPREISDKVRNSVHLTREEMQ
                     RWAKILGKNGWLGWGWPKEFGGPGWNAIQKHLFEEETALAGAPRVIPFGPVMVAPVIM
                     AFGSPEQQKHFLPGIASGEVWWSQGYSEPGSGSDLASLKTRAERVHRAGGDVYIVNGQ
                     KTWTTLGQYGDWIFCLVRTSSDGKPQTGISFLLIDMKSKGVTVRPIITMDGAHEVNEV
                     WFDNVEVPADQLIGEENKGWTYAKYLLAHERTNIADVNRAKRELERLKRIARVEGVYE
                     PGRFRDEIARLEVDVVALEMMVLRVLSAERSERGGGKQSLDIAGLLKIRGSEIQQRYS
                     ELMMLAAGPAALALRTEAMQAGWQAEHADSAAFVAPLAPTYFNMRKTTIYGGSNEVQR
                     NIVAQTVLG"
     gene            2406..>3150
                     /locus_tag="VFF72_12310"
     CDS             2406..>3150
                     /locus_tag="VFF72_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV93993.1"
                     /translation="MDFDFSDDQEMLRDSVRKWVEKSYDFERRRGIVKAGGFSREAWQ
                     GLADLGLLGLQVAEAQGGMGLGAVDAMVVMEELGRGLVLEPFAATSLVAASLLARAPA
                     GDSLLARIAGDAAIVVLAQEERHSRYRLGHVETKAAGSGDALRLTGTKIAVAGGGNAD
                     AFIVSARSSGSVDDPGGVGLYLVEKGAAGATLDSCATQDGASAADLHLVDARATLLLG
                     PGAAMPALEHAVDIGIAALCAEAVGAMDRL"
BASE COUNT          557 a         1078 c         1079 g          436 t
ORIGIN      
        1 caagatcgag aagatcggcg gcgacaaggt caagcaggtc gtcgtcgtgc gcagcggcat
       61 cgacagcgac acggcccgga agctgcagac cgccgtcaag cagagcaagc tcaaggtgca
      121 ggcgtcgatc cagggcgaca ccgtgcgcat cagcggcggc aagcgcgacg acctgcaggc
      181 agcgatcgcg ctggtgcgaa aggagctgcc cgatcagccc ctttcctaca tcaacttccg
      241 cgactgacat gcgaacgcct ttcatggcga ccgtgttcgc gctggcactg gccgcggccg
      301 ccagcgcgat ggcgcaatcg gtgtcgatgg gtggcagcct cggcagccac gcgctgctgg
      361 tgatcgacgg caaggcccga aacgtcgccg tcggcagcac cgtcgacggc gttcgcctcg
      421 tcagcgtcaa cggcaacgag gcggtcgtcg aggtcaaggg gcagcgggtc accgtcatgc
      481 tgggcggcgc gccggccaac ctcggcggcg cggcgagcga aggcagcggc cagaagatcg
      541 tcatcaccgc cggcagcggt ggccatttct tcacgaccgg gacgatcaac ggccgatcgg
      601 tgaacttcgt cgtcgacacc ggcgcgacga cgatcgccct gagcgaagcc gaagccgacc
      661 ggatcggcct cgactacaag cacgggcgaa tcagctacag cagcaccgcc aacggcgtcg
      721 tcgtcgcgca ccgggtgtcg ctgtcgtcgg tgcggatcgg cgatgtgcag gtctacaacg
      781 tcgccgcgac ggtgctgccg acgccgctgc ccatcgtcct gctcggcaac agctttctcg
      841 agcgcttcca gatgcgccgc gagaacgacc tcctcacgct cgacaagcgg tattgaacgg
      901 cgccggaacc ggccgccccg aatgcatggg cggtcacgac acgcgattcg ccgacgcagc
      961 accgaccgcc gcacgatcgc cgaaacccgg tccgactgtc gcatcgccga caggccgaat
     1021 cgcacggtcg tgctaaaaac ctcgcctgac ccgcgccaca tcccgaccgg agacttcgat
     1081 ggatctcacc ttcacgcctg aagagcaggc cttccgcgag caggtccgtg cctgggtcaa
     1141 ggacaacctg ccgagggaaa tcagcgacaa ggtgcgcaac tcggtgcacc tgacgcgcga
     1201 agagatgcag cgctgggcga agatcctcgg caagaacggc tggctcggct ggggctggcc
     1261 gaaggaattc ggcggcccgg gctggaacgc gatccagaag catctcttcg aggaagagac
     1321 cgcgctcgcc ggagcgccgc gcgtgatccc gttcggcccg gtcatggtgg cgcccgtcat
     1381 catggctttc ggctcgcccg agcagcagaa acacttcctg cccggcatcg ccagcggcga
     1441 ggtctggtgg agccagggct acagcgaacc cgggtcggga tccgacctcg cctcgctgaa
     1501 gacgcgcgcc gagcgggtgc atcgcgccgg cggcgacgtc tacatcgtca acggccagaa
     1561 gacctggacc acgctcggcc agtacggcga ctggatcttc tgcctcgttc gcacctcgag
     1621 cgacggcaag ccgcagacgg gcatcagctt cctgctgatc gacatgaaga gcaagggcgt
     1681 cacggtgcgc cccatcatca cgatggacgg ggcgcacgag gtcaacgagg tctggttcga
     1741 caacgtcgag gtgcccgccg accagctcat cggcgaggag aacaagggct ggacctacgc
     1801 caagtacctg ctcgcccacg agcgcaccaa catcgccgac gtcaaccgcg ccaagcgcga
     1861 gctcgagcgg ctgaagcgga tcgcaagggt cgaaggcgtc tatgagcccg gccgatttcg
     1921 cgacgagatc gctcgcctcg aggtcgacgt cgtggcgctc gagatgatgg tgctgcgcgt
     1981 gctttcggcc gagcgttccg agcgtggcgg cggcaagcaa agcctcgaca tcgccggcct
     2041 cctcaagatc cgcggcagcg agatccagca acgctacagc gagctgatga tgctggccgc
     2101 agggcccgcg gccctggcgc tgcgcaccga agcgatgcag gccggctggc aggccgagca
     2161 cgccgacagc gcagcgttcg tcgcgccgct ggcacccacc tacttcaaca tgcgaaagac
     2221 gacgatctac ggcggctcga acgaggtgca gcgcaacatc gtcgcccaga cggtgctcgg
     2281 ctgaaagccg aacacagtcc gggcgacgtg cccgcggcct ttggccgcgg gctcgcccgc
     2341 agagttgccg ctttccccgc cccgtccctc cggcagggaa ggggttccag ttagaaggac
     2401 gaatcatgga tttcgatttc agcgacgacc aggagatgct gcgcgactcg gtccgcaagt
     2461 gggtcgagaa gagctacgac ttcgaacgcc ggcgcggcat tgtcaaggcc ggcggcttct
     2521 cgcgcgaagc gtggcagggg ctcgccgatc tcggcctgct cggcctgcag gtggccgagg
     2581 cgcaaggcgg catgggcttg ggcgccgtcg atgcgatggt ggtgatggag gagctcggtc
     2641 gcggcctcgt cctcgagccg ttcgcggcga cctcgctggt tgccgccagc ctgctcgcgc
     2701 gagcgcccgc cggcgattcg ctcctggccc gcatcgccgg cgacgcggcg atcgtcgtgc
     2761 tggcccagga ggagcgccat tcccgctacc ggctcggcca cgtcgagacc aaggccgcgg
     2821 gcagcggcga tgcgctgcgg ctcaccggca cgaagatcgc cgtggcaggc ggcggcaatg
     2881 ccgatgcctt catcgtcagc gcgcgcagca gcggcagcgt cgacgaccca ggcggcgtcg
     2941 gcctctacct ggtcgagaaa ggcgccgccg gcgcgacgct cgattcctgc gcgacgcagg
     3001 acggcgccag cgccgccgac ctgcatctcg tcgacgccag ggcgacgttg ctgctcggcc
     3061 cgggcgcggc gatgccggcg ctcgagcacg cggtcgacat cggcatcgcc gcgctctgcg
     3121 ccgaggcagt cggcgccatg gatcgcctgg
//
LOCUS       DASRWS010000644         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_259084, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000644 DASRWS010000000
VERSION     DASRWS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259084"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFF72_12315"
     CDS             <1..845
                     /locus_tag="VFF72_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV93994.1"
                     /translation="WSSSSTFDWPAGRCRPEFRKPFMPRTPSAVLARVGPPVVVLVSV
                     VLLWELVVRAFEIPPWLLPAPSAIAARFVRTQDLWYHTGLTLIEAGSGFAISAVLGIA
                     MAAGIANSRLLERGIFPYVVVSNAIPIIAIVPLLNIWLGFGIATKIVIAAIITFFPIV
                     TNTTRGLKAADPRILDFMHSINATRLEVFSKVQLPSALPFIFAGFKIAASLSLVGAVV
                     GEFYGADRGLGYLVITAATQLRTDLLFVAIIVLAVIGIVTFTFFGYLERHAIHGAAEV
                     ERQR"
     gene            842..2161
                     /locus_tag="VFF72_12320"
     CDS             842..2161
                     /locus_tag="VFF72_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZV93995.1"
                     /translation="MISSPIGVPTLQIRNALLITGEEREEPFFGWIEVHGRRIGRVER
                     GDAPAIPGVRVIDAEGGALLPGLVNAHAHSHSSLTRGSAEGMTLERWIDTIEREQSRL
                     TDEDAYVAALATYGEALLSGTTSIVDMCLRPRPAMRAARALGIRVAIAPYVLDGRAFA
                     PTLDDVEALLRDKAARGGTVDIWVGLHDLESCADDTVRRGAQMARDFGTGLHLHCSES
                     RFSVDRTLRRTGMTPIAHLQALGALGPRTLLAHCVWADGDDRAILARTATRVAHCPHA
                     NLKLASGFAPVPAMIDAGIKVALATDGAKANNRLDMFDVMKFASLLHRGVALDASVIS
                     PAAVLGMATHGGALGQEGGSIAPGRVADLILVDVRQFHLQPAVPSTIETNLVHSARGS
                     DVRTVIVDGEVVVEDGQLVRLPMAPIVDSMRATADRLMAHDEISQPQ"
     gene            complement(2263..3333)
                     /locus_tag="VFF72_12325"
     CDS             complement(2263..3333)
                     /locus_tag="VFF72_12325"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HZV93996.1"
                     /translation="MTPFTYTAKPSRVVFGVGSLQFLGREIEMMGGKRALVLATPGHA
                     ESARRVAAMLGPRAVGVFAEARMHVPIETARAAREEARRLGADCAVAIGGGSTVGLGK
                     AVALEAEGDFAILAIPTTYAGSEMTPIYGLTEGGMKKTGRDVRVLPKTVLYDPELTLT
                     LPLPLSLTSIFNAIAHACEGLYADDGNPITALMAEEGIRAAASAIPRLSREPRDLDAR
                     CDALYAAWLCGSVLGSVAMGLHHKLCHTLGGTFNLPHAEVHTVILPHVLAYNAPAARE
                     AMSRIGRALGTGSQSASMGIFDLARTHGAPTALRDIGMPEAGLDRATDLAVQSQYPNP
                     RPLERGAIRELLQRAFDGTRPG"
     gene            complement(3535..>4422)
                     /locus_tag="VFF72_12330"
     CDS             complement(3535..>4422)
                     /locus_tag="VFF72_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZV93997.1"
                     /translation="LLEAIHRTASVSAAALEVGLSQPAASHALARMRRLLGDELFVRS
                     SQGMVPTWYGKKASTAVAGVLRTLREGLSAEKEFDPLTTERIFRVCMSDVGQLVLLPK
                     LLAHFRRHSPGARLHVEHFPKISQGRAVELGDVDLAIGHIQSLVAGFRQRTLFIERYV
                     CVASGDNVRFAGGMNLQAFSDSPHAMADSSGMAHWVVDDSFTKKGVTRRRGLVVPEFM
                     TLPFVIAGSEFVVTMPSRLAERFAKISPLRVMPPPVYLEPYPIRMFWHESAHKDPGNV
                     WLRRALVYLFARAPAPKSA"
BASE COUNT          775 a         1419 c         1410 g          818 t
ORIGIN      
        1 gatggagttc atcgagcacg ttcgactggc ccgccgggcg ctgcaggcct gaatttcgga
       61 agccgttcat gccgcgcact ccttcggcag ttctcgctcg cgtgggcccc ccggtggtcg
      121 tcctcgtgtc ggtggtgctc ctgtgggagt tggtcgtgcg tgcgttcgaa atcccgccct
      181 ggctactgcc ggcgccgtcg gcaatcgccg cacgcttcgt gcgcacccag gacctctggt
      241 accacaccgg tctcaccttg atcgaggcgg gatcgggctt cgcgatttca gcagtactgg
      301 ggatcgccat ggccgcgggc atcgcgaatt cgcgtttgct cgagcgcggc atctttccct
      361 acgtggtcgt ctcgaacgcc atcccgatca tcgccatcgt cccgttgctc aacatctggc
      421 tcggctttgg cattgcgacc aagatcgtca ttgcggcgat catcacgttc tttcccatcg
      481 tcacgaacac gacacgtggc ctcaaggcgg ccgacccgcg catcctcgat ttcatgcact
      541 ccatcaatgc gacgcgcttg gaagtgttct cgaaagtaca gttgccaagc gcgctcccat
      601 tcatctttgc cggcttcaag atcgccgcgt cgcttagcct tgtcggcgcc gtcgtaggtg
      661 agttctatgg ggctgatcgc ggcctcggct accttgtcat cacggccgcg actcaactgc
      721 ggaccgatct gctgttcgtc gcgatcatcg tcctggctgt gatcggcatc gtgacattca
      781 ccttcttcgg ctatctcgag cgccacgcca tacacggtgc ggctgaagtg gaacgtcaga
      841 gatgatctct tctccgatcg gtgtcccgac gcttcagatt cgcaatgcgc ttttgatcac
      901 cggcgaggaa agggaagagc catttttcgg gtggatcgaa gtgcacggca ggcgtatcgg
      961 cagggtcgag cgtggcgatg caccagcgat cccgggcgtg cgcgtcatcg acgccgaggg
     1021 cggggccctc ctgccggggc tcgtcaatgc ccatgctcat tcacactcaa gcctgacacg
     1081 cggcagcgcc gagggaatga ccctagaacg ctggatcgac accatcgagc gcgagcagag
     1141 tcggctgact gacgaagacg cctacgtcgc cgcgctggcg acctacgggg aggcgctgct
     1201 ttcgggcacg acctcgatcg tcgatatgtg cctgcgaccg cgcccggcga tgcgtgcggc
     1261 acgcgcgctc ggcatccggg tcgccatcgc gccctatgta ctcgatggcc gtgcctttgc
     1321 tccgactctc gacgatgtcg aggcgcttct tcgcgacaag gcggcgcggg gtggaaccgt
     1381 agacatctgg gtcgggctgc acgatctcga gtcctgcgcc gacgacacgg tgcgccgcgg
     1441 cgctcagatg gcgcgagact tcggcacggg cttgcatctg cattgctccg aaagccgctt
     1501 ttcggttgat cgaacgcttc gacgtacggg gatgaccccc atcgcacacc tgcaagcgct
     1561 cggcgcgctc gggcctcgca cgctcctcgc gcattgcgtc tgggccgatg gggacgatcg
     1621 ggcgattctc gcccgtaccg caacgcgcgt tgcccactgt ccgcacgcca atctcaagct
     1681 cgcgtcaggt ttcgctcccg taccggcgat gatcgacgcc ggcatcaagg ttgctctggc
     1741 gaccgatggg gcaaaggcca acaatcgtct cgacatgttc gatgtcatga agttcgcctc
     1801 gctactgcac cgaggcgtcg cgctcgatgc ctcggtgata tcgcctgccg cagtgcttgg
     1861 catggcgacc catggcggtg cgctcggtca agaaggcggc agcatcgcgc ccggtcgagt
     1921 cgccgacctc atcctcgtcg acgtgcgtca gttccatttg cagccagcgg ttccttcaac
     1981 catcgagacc aatctcgtgc actcggcgcg tggctccgac gtcaggacgg tgattgtcga
     2041 cggcgaggtc gtcgtcgaag acgggcagct tgtccgcctg ccgatggcgc caatcgtcga
     2101 ttcaatgcga gccactgctg atcggttgat ggcgcacgat gaaatatctc aaccgcagtg
     2161 accggcagcg tccaggtcga tgctgcccac ataggcaata tcgccacccg tgacaggcaa
     2221 aatgatgcct tcacgagcaa ggtgatcaga ccaaggtcga catcaaccag gccgcgtgcc
     2281 gtcgaaggcg cgctgcaaga gttcgcggat cgcccctcgc tcaagcggac gagggttcgg
     2341 gtactggctc tgcaccgcga ggtccgtggc acggtcgaga ccagcctctg gcatgccgat
     2401 gtctcgcagc gccgtgggtg ccccgtgcgt gcgcgcgaga tcgaagatac ccatggacgc
     2461 agactgtgat cccgtaccca atgcgcggcc aatgcgggac atcgcttctc gcgccgcagg
     2521 cgcgttgtac gcaaggacat ggggcaggat gaccgtgtgc acctcggcgt gcggaagatt
     2581 gaatgtgccg ccgagcgtgt ggcacagctt gtggtgcaag cccatggcca cgctgccgag
     2641 cactgagccg cacagccagg cagcgtagag cgcgtcgcag cgcgcgtcga gatcgcgggg
     2701 ctcgcgactg agacgcggaa ttgcgctggc ggcggcccgg atgccctctt cggccatcag
     2761 tgccgtgatc gggttgccat cgtctgcata caggccttcg caggcgtggg cgatggcgtt
     2821 gaagatgctc gtcagcgata gcggcagcgg cagggtgagc gtcagctcgg gatcgtagag
     2881 aacggtcttc ggcagcacgc gtacgtcacg tccggtcttc ttcatcccgc cctcggtcag
     2941 gccgtagatg ggcgtcattt cgctgccggc gtacgttgtc gggatcgcga ggatcgcaaa
     3001 gtcgccttcg gcctcgaggg cgaccgcctt gccgagcccc accgtcgagc cgccgccgat
     3061 ggcgaccgcg cagtcggcgc ccagacgtct tgcttcctct cgggccgccc gcgccgtctc
     3121 gatcggcacg tgcatgcgag cctcggcgaa gacaccgacc gcgcgcggac cgagcatcgc
     3181 ggcgacgcgg cgggcagatt ccgcatggcc gggcgtcgcc agcacgagcg cacgcttgcc
     3241 gcccatcatt tcgatctcgc gcccgaggaa ctgcagggac ccgacgccga agacaacccg
     3301 agacggcttg gcggtgtagg tgaaaggcgt cacttgatgc tcgatcctat cgcgtcgaac
     3361 ttgccccaga cttacccact tggctgactt cgatcctcga cccgaccatc tcaccgaccg
     3421 ggggccgacg actctggtcg tcgacgatgt gttgattgac ttggttgcca acaatggaag
     3481 gccggtacga ccaaaagagt cgaaggtact cgatacgccg agcgcggcaa tccttcacgc
     3541 cgacttcggt gccggggcgc gggcgaacag gtaaaccaga gctcgtcgca gccaaacgtt
     3601 gccgggatct ttatgcgcgc tctcgtgcca gaacatgcga atcgggtacg gctcgagata
     3661 aacgggggga ggcattacac gcaagggtga gattttggcg aagcgctcgg caagtcggct
     3721 tggcatcgtc acgacgaatt ccgagcccgc gatgacaaac ggcagcgtca tgaactcagg
     3781 aacgacaagg ccccgacgcc tcgtcacgcc cttcttggta aacgaatcgt cgacgaccca
     3841 atgggccatg ccgctgctgt cggccatggc atgcggtgaa tcgctgaatg cctggaggtt
     3901 cataccccca gcgaaacgga cgttgtctcc tgatgcgacg cagacgtagc gctcgatgaa
     3961 aagggtccgc tggcgaaatc cggcgacgag cgactggata tggccgatcg cgaggtcgac
     4021 gtcgcccaat tcgacggcgc ggccctgact gatcttcggg aagtgctcga cgtgcaaccg
     4081 ggcgccgggc gaatgacggc gaaagtgcgc cagcagtttg ggtaatagaa cgagttggcc
     4141 gacgtcgctc atgcagacgc gaaagatccg ctcggtcgtc aaggggtcga attccttctc
     4201 ggcagacagg ccctcgcgca gcgtgcgcag cacaccggcc accgcggtgc tggccttctt
     4261 gccgtaccag gtggggacca tgccctgtga cgagcgcaca aaaagctcgt cgcccagaag
     4321 cctgcgcatt cgcgccaagg cgtggctcgc cgccggttgg gatagaccaa cctcgagagc
     4381 ggccgcggaa acgcttgcag tgcggtgaat tgcctccagc ag
//
LOCUS       DASRWS010000645         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2545200, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000645 DASRWS010000000
VERSION     DASRWS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545200"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFF72_12335"
     CDS             complement(<1..616)
                     /locus_tag="VFF72_12335"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HZV93998.1"
                     /translation="MTNLKKLIRDKKTVWAAGAFDALSARLIQEAGFDALLTSGYGIS
                     SSFLGEPDTEFYTMTENLAVTRNVCNAVTIPVIADIDTAYGNAVSAMRTMREFENAGA
                     AAVIVEDQVSPKRCPCSSSQIDVVPMEDHVGKIRAMVAARKNTDVLIIARTDAVEPAE
                     AIRRGKAYVAAGADIIQPISRCFKNFEGLQAMREGCGVPLSLQLL"
     gene            complement(613..1149)
                     /locus_tag="VFF72_12340"
     CDS             complement(613..1149)
                     /locus_tag="VFF72_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HZV93999.1"
                     /translation="MAMKNLRGRVAFKFEEVDFDVDQIVGVKNIKTTNIDELSKAAMQ
                     EYDPAFATVVKPGDLLVGNHNFGYGHPHYPPMKAMRHLGISGVIAESFSPGYWRGEIS
                     MGFPQVSCPGILAFVERWDEVEVDWAAQHIRNLTQNTTIAFEPLPKADLAMLEAGGLE
                     TYLKKRFQTDNALKELSP"
     gene            complement(1161..2423)
                     /locus_tag="VFF72_12345"
     CDS             complement(1161..2423)
                     /locus_tag="VFF72_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZV94000.1"
                     /translation="MGFTITEKILARTAGLPSVKAGDEIMARPDFVLAYDFPGYTDVI
                     FKQLKNDFGITKMAEPERFGIFIDHMVPAISPAEEELHKGTREWTAANDVNLYERKGI
                     GHQVSSEVGYATPGAFAVHFDGHVSQLGTFGTLAMGLRRNVLEAFAMEKVSLRVPQTT
                     RVDLVGKLQPGVMARDVFHHMVRVLGPSSCRFQVLEIGGPAVADMSNDALQTITGLAM
                     FTGAVTAIVNPDARRLAYALPRARKKLEPVYSDDDASYAARHTIDLTTLEPIIVIPPS
                     PANTRDLKDYLGLDVQAGYLGSCASGRIEDMRAAAGVLRGRKVAQGFQLHVVPTSQEI
                     MVAAAREGLIEVLAEAGAFVSSPSCDYCFGRIATMAPGQRAVSTGTLNVRGRMGSPDS
                     EIYLCNAAVIAASAIEGKITDPRPYLQG"
     gene            complement(2460..>3081)
                     /locus_tag="VFF72_12350"
     CDS             complement(2460..>3081)
                     /locus_tag="VFF72_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZV94001.1"
                     /translation="VEAGSEHALAASMATLRAIGKKHGIEIENAAARVMRSRPFNPVR
                     GMLGAEGQRWVPIHAVFPLSEAERVVQANEAYFESQQAMMRQHGIIVSHLTMTVGNEF
                     FLEPAFYWPDEITPLHRKSLGDDVVGPWLGRPANLPAREAVIALRKGTQQLYLSLGGV
                     NWQIGRDYPLQQAMQPESWDLLVALKRAVDPQGLMNPGSLGLRAAA"
BASE COUNT          473 a          998 c         1060 g          550 t
ORIGIN      
        1 ccagcagctg cagcgacagc ggcacgccgc agccttcgcg catcgcctgc aggccttcga
       61 agttcttgaa acagcggctg atcggctgga tgatgtcggc gccggccgcc acgtaagcct
      121 tgccgcggcg gatggcctcg gccggctcga ccgcgtcggt gcgcgcgatg atcagcacat
      181 cggtgttctt gcgagccgcg accatcgccc tgatcttgcc gacatggtct tccatcggca
      241 cgacgtcgat ctggctcgac gagcaggggc atcgcttcgg cgacacctgg tcctcgacga
      301 tgactgccgc ggcaccggcg ttctcgaact cgcgcatcgt gcgcatcgca ctgacggcgt
      361 tgccgtaagc ggtgtcgatg tcggcaatga cggggatcgt caccgcattg cagacgttgc
      421 gcgtgacggc gaggttctcg gtcatcgtgt agaactcggt gtccggctcg ccgagaaaag
      481 acgacgagat gccgtagccc gaggtcagca gcgcgtcgaa accggcctcc tggatcaagc
      541 gcgcgctcag ggcatcgaag gcgccggcgg cccatacggt cttcttgtcg cggatcagtt
      601 tcttcaggtt cgtcatggcg aaagctcctt cagggcgttg tcggtctgga atcgcttctt
      661 caggtaagtc tcgaggccgc cggcctcgag catggccaag tcggccttcg gaagcggctc
      721 gaaggcgatg gtggtgttct gcgtcaggtt gcggatgtgc tgcgccgccc agtccacttc
      781 gacctcgtcc cagcgctcca cgaacgccag gatgccggga cacgagacct gggggaaacc
      841 catgctgatc tcgccgcgcc agtagccggg ggagaacgac tcggcgatga cgccggagat
      901 gccgaggtgg cgcatcgcct tcatcggcgg atagtgcgga tggccgtagc cgaagttgtg
      961 gttgcccacc agcaggtcgc ccggcttcac cacggtggcg aaggcggggt cgtattcctg
     1021 cattgccgcc ttgctcagct cgtcgatgtt ggtggtcttg atgttcttca cgccgacgat
     1081 ctggtcgacg tcgaagtcga cctcctcgaa cttgaaggcg acgcggccgc gcaggttctt
     1141 catggccatt gctcgttcct tcagccttgc aggtagggcc gcgggtcggt gatcttgcct
     1201 tcgatcgccg atgccgcgat cacggcggcg ttgcacaggt agatctccga gtcggggctg
     1261 cccatgcggc cgcgcacgtt gagcgtgccg gtggacacgg cgcgctggcc gggcgccatg
     1321 gtcgcgatgc ggccgaagca gtagtcgcaa gagggcgacg agacgaaggc gccggcctcg
     1381 gcaaggacct cgatcaaccc ctccctggcc gccgcgacca tgatctcctg gctggtcggc
     1441 acgacgtgca gctggaagcc ctgggccacc ttgcggccgc gcaggacgcc ggccgcggcg
     1501 cgcatgtcct cgatccggcc cgaggcgcag gagccgaggt agccggcctg cacgtcgagc
     1561 cccaggtagt ccttcaagtc gcgcgtgttg gccgggctcg gcgggatgac gatgatcggc
     1621 tccagcgtcg tcaggtcgat ggtgtggcga gccgcgtagc tcgcgtcgtc gtcgctgtag
     1681 accggctcga gcttcttgcg ggcgcgcggc agggcgtagg ccaggcgcct ggcgtcgggg
     1741 ttgacgatcg ccgtcaccgc gccggtgaac atcgccaggc cggtgatggt ctgcagggca
     1801 tcgttcgaca tgtcggccac cgcggggccg ccgatctcga gcacctggaa gcggcaagac
     1861 gacgggccga gaacacgcac catgtggtgg aacacgtcgc gcgccatcac gccgggctgc
     1921 agcttgccga cgaggtcgac gcgggtcgtc tgcggaacgc gaagcgacac cttctccatg
     1981 gcaaaggcct cgagcacgtt gcggcgcagc cccatggcca gcgtgccgaa ggtgccgagc
     2041 tggctcacat ggccatcgaa atggaccgcg aaggcgcccg gcgtggcgta gcccacttcc
     2101 gatgaaacct ggtggccgat gcccttgcgt tcgtagagat tcacgtcgtt ggcggccgtc
     2161 cactcgcgcg tgcccttgtg cagctcttcc tcggccggac tgattgccgg gaccatgtgg
     2221 tcgatgaaga tgccgaagcg ctcgggctcg gccatcttcg tgatgccgaa gtcgttcttc
     2281 aactgcttga agatcacgtc ggtgtagccc gggaagtcgt aggccaggac gaagtcgggc
     2341 ctggccatga tctcgtcgcc agccttcacc gacggcaggc cggcggtgcg cgcgaggatc
     2401 ttttcggtga tggtgaatcc catggcgtct ggttccgaag aggtgtcgaa ggattgccct
     2461 caggccgctg cgcgcaggcc cagggagccg gggttcatga ggccctgcgg atccacggcg
     2521 cgcttcagcg cgaccagcag atcccagctc tccggctgca tcgcctgctg cagcggatag
     2581 tcgcggccga tctgccagtt gacgccgccc aggctcaggt agagctgctg cgttcccttg
     2641 cgcaaggcaa tcaccgcttc gcgggccggc aggttcgcgg gccggcccag ccatgggccg
     2701 accacgtcgt cacccaggct cttgcgatgc agtggcgtga tctcgtcggg ccagtagaac
     2761 gcgggttcga ggaagaactc gttgccgacg gtcatcgtca ggtgcgagac gatgatgccg
     2821 tgctgccgca tcatggcttg ctgcgactcg aagtacgcct cgttcgcctg caccacgcgc
     2881 tcggcctcgc tcagcgggaa caccgcgtgg atgggcaccc agcgctgccc ttcggcgccc
     2941 agcatgccgc gcaccgggtt gaacggccgc gagcgcatga cgcgagcggc tgcgttctcg
     3001 atctcgatcc cgtgcttctt gccgatcgcg cgcagcgtcg ccatgctggc ggcaagggcg
     3061 tgctcgctgc ccgcttcgac g
//
LOCUS       DASRWS010000646         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4270835, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000646 DASRWS010000000
VERSION     DASRWS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4270835"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1873)
                     /gene="lpdA"
                     /locus_tag="VFF72_12355"
     CDS             complement(47..1873)
                     /gene="lpdA"
                     /locus_tag="VFF72_12355"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZV94002.1"
                     /translation="MSVEVRVPDIGDFKDVAVIEVLVKPGDSVKVEQSLITIESDKAS
                     MEIPSSRAGVVKELKVKLGDHVNEGSVILTIDEAAASQATGAATAAAPTAGKSATAAS
                     PPASVAAPAAPTPRPASARTQPSAASAGGDITCDLLVLGAGPGGYSAAFRAADLGLKV
                     VLVERYATLGGVCLNVGCIPSKALLHVAAVIDEAAHFADVGVAFGAPKVDTARLVQHK
                     NKVVGRLTGGLAQMAKMRKVTVVTGAGRFIDPNHVAVDLAAGGSTTVGFARAIVAAGS
                     EAVKLPFMPKDDPRIVTSTGALELPARPQRMLIVGGGIIGLEMGTVYSTLGARLDVVE
                     MLDGLMQGADRDLVKVWQRLNAPRFDHIMLKTKTVSAESRADGVLVRFEGEGALKEQL
                     YDLVLQAVGRVPNGKRIGAEAAGVDVGERGFIPVDIQMRTNVPHIFAVGDIVGQPMLE
                     HKAVHEAHVAAEIAAGEQLGDDKLAKTAFDARVIPSVAYTDPEIAWVGITEDEAKARG
                     VKVKKGLFPWTASGRAIANGRDEGFTKLLFDEATHVVLGGGIVGTHAGDMIGEIALAI
                     EMGADAIDIGKTIHPHPTLGESIGLAAEAFEGVCTDLPPVRR"
     gene            complement(1953..>3082)
                     /locus_tag="VFF72_12360"
     CDS             complement(1953..>3082)
                     /locus_tag="VFF72_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZV94003.1"
                     /translation="GDKVAEGTLLLLIDADGAAAAPAPAAAPALAAAPAPAAESKPAP
                     APAPAPAPAPIVAAPTPAVPGPAGLPHASPSVRKFARELGVDLTRVAGKGPKGRITQE
                     DVQNFVKGVMSGSVSTGAASKGSGGGAGLDLLPWPSLDFSKFGETELLALSRIKKISG
                     PNLHRNWVMIPHVTQFDECDVTDLEQFRKDTNAANAKSGMKLTMLAFVIKASVAALKK
                     FPAFNASLDAEGANLILKKYYHIGFAADTPNGLVVPVIKDADSKGVLQISAEMGELAK
                     KARDGKLSPADMSGGCFSISSLGGIGGRYFTPIINAPEVAILGVCRSVMEPVWDGKAF
                     QPRLMLPLSLSWDHRVVDGASAARFNAFLIQILGDFRRVLL"
BASE COUNT          468 a         1051 c         1058 g          505 t
ORIGIN      
        1 ggagaggggc cgcgaacgcg gccccttttc cgtttgccat tgcgcgtcag cgtcgaaccg
       61 gcggcaggtc ggtgcagacg ccctcgaacg cctccgcagc caggccgatc gattcgccca
      121 gcgtcggatg cggatggatc gtcttgccga tgtcgatcgc atcggcgccc atctcgatgg
      181 ccagcgcgat ctcgccgatc atgtcgccgg catgcgtgcc gacgatgcca ccaccgagga
      241 ccacatgcgt cgcctcgtcg aagagcagct tggtgaagcc ttcgtcacga ccgttggcga
      301 tggcccggcc cgatgccgtc cacggaaaga ggcccttctt caccttgact ccgcgcgcct
      361 tcgcctcgtc ttcggtgatg ccgacccagg cgatctccgg atcggtatag gcgacgctcg
      421 ggatcacccg cgcgtcgaac gcggtcttcg ccagcttgtc gtcgcccagc tgctcgccgg
      481 ccgcgatctc ggcggcgacg tgcgcctcgt gcacggcctt gtgctcgagc atcggctggc
      541 cgacgatgtc gcccacggcg aagatgtgcg gcacgttggt gcgcatctgg atgtcgaccg
      601 ggatgaagcc gcgctcgccg acgtcgacac cggcagcctc ggcgccgatg cgcttgccgt
      661 tgggtacgcg gccgacggcc tgcagcacca ggtcgtagag ctgctccttg agcgcgcctt
      721 cgccttcgaa tcgcacgagc acgccgtcgg cgcgcgactc ggcgctcacc gtcttcgtct
      781 tcagcatgat gtggtcgaag cgtggcgcgt tcagcctttg ccagaccttg acgaggtcgc
      841 ggtcggcgcc ctgcatgagg ccatcgagca tctcgacgac atcgagccgg gcgccgagcg
      901 tcgagtagac ggtgcccatc tcgaggccga tgattccgcc gccgacgatg agcatccgct
      961 gcggacgcgc cggcagctcg agggcgccgg tcgaagtgac gatgcgcgga tcatccttgg
     1021 gcatgaaggg cagcttcacc gcctccgatc cggccgcgac gatggcccgc gcaaagccga
     1081 cggtggtgct tccgcccgcc gcgaggtcga ccgcgacgtg gttcgggtcg atgaagcgcc
     1141 ccgcgcccgt gacgaccgtg accttgcgca tcttcgccat ctgcgcaagg ccacccgtca
     1201 ggcggccgac gaccttgttc ttgtgctgca cgagcctggc ggtgtcgacc ttcggcgcgc
     1261 cgaaggcgac gccgacgtcg gcgaagtgcg ccgcctcatc gatcacggcg gcgacgtgca
     1321 ggagggcctt cgacggaatg cagccgacat tgaggcagac gccgccgagc gtggcgtagc
     1381 gctcgacgag gacgaccttc aggccgaggt cggcggcgcg aaacgccgcc gaatagccac
     1441 ccgggccggc gccgagcacg agcaggtcgc acgtgatgtc gccgccggcc gaggcggccg
     1501 atggctgcgt ccgtgctgac gccgggcggg gcgtcggcgc ggcaggggcc gccaccgagg
     1561 ccggcggcga tgccgcggtc gcggatttgc ccgcagtcgg tgcggcggcc gtcgccgcgc
     1621 ccgtcgcttg cgacgcggcg gcttcgtcga tcgtcaggat gaccgatcct tcgttgacgt
     1681 ggtcgccgag cttgaccttc aactccttga cgacgccggc gcgcgacgac gggatctcca
     1741 tcgacgcctt gtcgctttcg atcgtgatca ggctctgttc gaccttgacc gagtcgccgg
     1801 gcttgaccag gacctcgatg acggccacgt ccttgaagtc gccgatgtcc ggaaccctga
     1861 cctcgacgct catgctgcct ccccgttcgc cgctgcggcg acgccgggtc gcgacggcgg
     1921 catcgagccc gtgcagcgcg cagcgccgcc gctcatagca gcacccggcg gaagtcgccg
     1981 aggatctgga tcaggaaggc gttgaagcgc gccgccgatg cgccgtcgac gacgcgatgg
     2041 tcccagctga gcgacaacgg cagcatgagg cgcggctgga aagccttgcc gtcccacacc
     2101 ggctccatga cgcttcggca gacgccgagg atcgcgactt cgggcgcgtt gatgatcggc
     2161 gtgaagtagc ggccgccgat gccgccgagc gacgagatcg agaagcagcc gccgctcatg
     2221 tcggccggcg aaagcttgcc gtcgcgtgcc ttcttcgcca actcgcccat ctcggccgag
     2281 atctgcagca cgcccttgct gtcggcgtcc ttgatcaccg gcaccacgag gccgttcggc
     2341 gtgtcggcgg cgaagccgat gtggtagtac ttcttcagga tcaggttggc gccttccgca
     2401 tcgagcgagg cattgaaggc cgggaatttc ttcagcgcgg caacgctggc cttgatgacg
     2461 aaggccagca tggtcagctt catgccggac ttggcgttgg ctgcattggt gtccttgcgg
     2521 aattgctcca ggtcggtcac gtcgcattcg tcgaattgcg tgacgtgcgg gatcatgacc
     2581 cagttgcggt gcaggttcgg gccgctgatc ttcttgatgc gcgacagcgc cagcaattcg
     2641 gtttcgccga acttggagaa gtcgagcgac ggccacggca acaggtcgag gcctgcgccg
     2701 ccaccgctgc ccttggatgc cgcgccggtc gagaccgaac cggacatcac acccttgacg
     2761 aaattctgca cgtcttcctg cgtaatgcgg cccttggggc ccttgccggc aacgcgcgtc
     2821 agatcgacgc cgagctcgcg tgcgaacttg cgcaccgaag gcgaagcatg cggcaagccg
     2881 gccgggccgg gtactgcagg cgtcggcgct gcaacaatcg gagcgggtgc cggcgcaggt
     2941 gcaggagccg gtgcaggctt gctctcagcg gcaggcgccg gagcagccgc cagcgcgggg
     3001 gccgcagcgg gcgcaggcgc ggcagcagcg ccgtcagcat cgatcagcag caacagcgtg
     3061 ccctcggcaa ccttgtcgcc ga
//
LOCUS       DASRWS010000647         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_539980, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000647 DASRWS010000000
VERSION     DASRWS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539980"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFF72_12365"
     CDS             <1..694
                     /locus_tag="VFF72_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94004.1"
                     /translation="PSFDIASQQNATVLLTPTTGGTLANGAVLIEQGSYTATPNTNVR
                     VRVVAAVTGGATVAASAGATPIGTVTSPSVGAYTVVPSGAALNITVNGASVAAPATPL
                     AAGSDETLLVYGNAAAPVANLIADDNHLPTVVTNFKIRLVNGLTGAAPPLTLNANFGV
                     VASNVAPGTASAYGVVGASVATRLDVLSPSSPTPLYSESNLNVPGNAVYTLFMLGDAG
                     AAKPIVLLQRDH"
     gene            862..1197
                     /locus_tag="VFF72_12370"
     CDS             862..1197
                     /locus_tag="VFF72_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94005.1"
                     /translation="MIAATVLALAASLAACAAPSVSASASMPREVDVRIIVKLLQPSG
                     DTAAIAADAARRAGVEVTYAAAVSGVWHALVLHCPDAAACTAATVRLRDSGAYADVEI
                     DGRKRRAVM"
     gene            1247..3403
                     /locus_tag="VFF72_12375"
     CDS             1247..3403
                     /locus_tag="VFF72_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZV94006.1"
                     /translation="MRKRLMSAAALAAVLLLPIAAQAPRAQGVVAAPAAVSARARVIV
                     KYRADSPLLKKQAMTAAGRRNLQVQALGDRIGLALTSGRGITARSSVVFADGISSSDL
                     AARLSAQSDVEYAVVDGRKHIVAVPNDTYYASGPALGASSGGPVVGQWYLKPPGAAGS
                     STTRTAPSAINAEQAWDVLAAAGVTGSSVVVAVIDTGVRFDHVDLQGGNVLPGYDMVS
                     ADTDGSFTTANDGNGRDSDASDPGDWVTQAESSGSGPLSGCAVEATSSWHGTQTLGLI
                     GAATNNGAGIASVGQGQVKVMPVRALGKCGGYDSDIVAAMYYAANAADSGASTDGTGT
                     YPVNPTPAKVISMSLGGTGSCSALYQDAMAAMTAAGVTVVAAAGNESATSADTPGNCP
                     GVIAVAALRSLGDKVGFSNIGTNVSIAAPGGNCGNNDGTAASPDQNACLYPIMSTSNS
                     GATTPVAASAGGSIYSDGYADPSLGTSFSTPLVAGTAALMLAVQPSLTPAEIKALLQS
                     SARPFPNSGSTLVTGSTVCTTTNGSTSTNCYCPNTAPLVCGAGMLDAHAAVLAALGVQ
                     ARITVDTATPTAGQPVSLSSNSLVNGNQTIASYAWTITSAGGTGATITGAANASSVTV
                     TPTGAGTFTVQLVVTDALGVTSTASTSVAVAATSSGGGPTTGPSSTGSSSGGGGAIGA
                     GWLLLLLAAVAALAAEERLQRRRRATIASVPATRRG"
     gene            complement(3375..3926)
                     /locus_tag="VFF72_12380"
     CDS             complement(3375..3926)
                     /locus_tag="VFF72_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94007.1"
                     /translation="MDLAFDLGEIGIDIDIDIGTRTNSTHDRGQHDRKDVEGIVLRLI
                     TLLLVVANLLFFGFTRGWFDGMFGLSSIGDREPERVASQIRPGNITLMPMPRASAPAS
                     IARAGCFEAGPVASADAASAEATLQATLPAGSWTDIANAGTAGVQHTYRVASADPALA
                     TRLAALRLGTSGQGFSPCASPAH"
BASE COUNT          639 a         1447 c         1410 g          603 t
ORIGIN      
        1 cccgtccttc gacatcgcca gccagcagaa cgccacggtg ctcctgacgc cgaccaccgg
       61 cggcacgctg gccaacggcg cggtgctgat cgagcagggc agctatacgg ccactcccaa
      121 caccaacgtc cgcgtccgcg tcgtcgccgc ggtgaccggc ggagcgacgg tcgccgcgtc
      181 ggccggcgcg acgccgatcg gcaccgtcac ctcgccttcg gtcggcgcct acaccgtcgt
      241 gccctccggt gcggcgctca acatcaccgt caacggcgca tcggtcgccg cgccggcgac
      301 gcctcttgcc gccggcagcg acgagacgct gctggtctac ggcaacgcgg cggcgccggt
      361 ggccaacctg atcgccgacg acaaccacct gccgacggtc gtcaccaact tcaagatccg
      421 cctcgtcaac ggcctgaccg gtgcggcgcc gccgctcacg ctcaatgcca atttcggtgt
      481 cgtcgcgagc aacgtggcgc cgggcacggc gtcggcgtac ggcgtcgtcg gcgccagcgt
      541 ggcgacgcgc ctcgacgtgc tgtcgccctc gagcccgacg ccgctctatt cggagtccaa
      601 cctcaacgtg ccgggcaacg ccgtctacac cctgttcatg ctgggcgatg ctggcgcggc
      661 gaagccaatc gtcttgctgc agcgcgacca ctgaccgcgc cctgaccgcg cgcggcgtgc
      721 ggtaattcgc ggccgcgcgt gtcggccggt aaggcttggt aaaggtggcc ggcgcgattt
      781 gcgccggcct ctaaactggc tcgcatccgt gtgttcgcga gccgttcctt ggcccttgcg
      841 tcgctccttt catcgaaggt cgtgatcgcg gcgaccgtgc tggcgctcgc tgcgtcgctc
      901 gccgcatgcg ctgcgccgtc ggtctcggca tcggcttcga tgcctcgcga ggtcgacgtg
      961 cgaatcatcg tcaagctcct gcagccgtcg ggcgataccg cggcgatcgc cgccgacgcc
     1021 gcccggaggg ccggcgtcga ggtgacgtat gccgccgcgg tgagcggcgt ctggcacgcg
     1081 ctcgtcctgc actgtccgga cgccgcggcg tgcacggcag ccaccgtgcg cctgcgcgac
     1141 agcggtgcct acgccgacgt cgagatcgat ggccgcaagc gccgggcggt gatgtgatcg
     1201 gaaaactggt cgatgcccgg tcgcgggcgc attcgggagg ggttcgatgc gaaagagact
     1261 gatgagcgcc gccgcgctcg ccgcggtgct tctgttgccg attgcggccc aggcgccgcg
     1321 cgcccagggg gtcgtggccg cgcccgcagc ggtgagcgcg cgcgcgcgcg tcatcgtcaa
     1381 gtaccgcgcc gactcgccgc tcctgaagaa gcaggcaatg accgcggccg gtcgccgcaa
     1441 cctgcaggtg caggcgctcg gcgaccggat cggcctggcc ttgacgtcgg gccgcggcat
     1501 caccgcgcgc tcgagcgtcg tcttcgccga cggcatcagc tcgagcgatc tcgcggccag
     1561 gctctcggcg caaagcgacg tcgaatacgc cgtcgtcgac ggccgcaagc acatcgtcgc
     1621 cgtgccgaac gacacctact acgcctccgg cccggccctc ggcgcgtcga gcggcggccc
     1681 ggtggtgggc cagtggtacc tgaagccgcc gggcgcggcc ggaagcagca cgaccaggac
     1741 ggcgccctcg gccatcaacg ccgagcaggc ctgggacgtg ctcgcagcgg ccggcgtcac
     1801 cgggtcgagc gtcgtcgtcg cggtcatcga caccggcgtt cgcttcgacc acgtcgatct
     1861 ccagggcggc aacgtgctcc ccggctacga catggtgagc gccgacacgg atggcagctt
     1921 cacgacggcg aacgacggca acggccgcga cagcgatgcc agcgatccgg gcgactgggt
     1981 gacgcaggcc gagtcgagcg gatcggggcc gctttcgggg tgcgctgtcg aggccacgag
     2041 ctcgtggcac ggcacgcaga cactcggcct catcggcgcg gcgaccaaca acggcgccgg
     2101 catcgccagc gtcggccagg gccaggtcaa ggtcatgccg gtgcgggcgc tcggcaaatg
     2161 cggcggctac gactccgaca tcgtcgccgc gatgtactac gcggcgaacg ccgcggacag
     2221 cggcgccagc acggacggta ccggcacgta tccggtcaac ccgacgccag ccaaggtgat
     2281 cagcatgagc ctgggcggca ccggctcctg cagcgccctc taccaggacg cgatggcggc
     2341 catgacggcg gcaggcgtca cggtcgtcgc cgctgccggc aacgaaagcg cgacgtcggc
     2401 cgacacgccc ggcaactgcc cgggcgtgat cgccgtcgcg gcgctgcgca gcctcggcga
     2461 caaggtcggc ttttccaaca tcggcaccaa cgtgtcgatc gccgcgccgg gcggcaactg
     2521 cggcaacaac gacggcaccg cggcgtcgcc ggaccagaac gcctgcctct atccgatcat
     2581 gtcgacgagc aactcgggcg cgacgacgcc ggttgcggcc tcggcaggcg gctcgatcta
     2641 cagcgatggc tatgccgatc cttcgctcgg cacgagcttc tcgacaccgc tcgtcgccgg
     2701 caccgcggcg ctcatgctgg cggtgcagcc atcgctgacg ccggccgaga tcaaggccct
     2761 gctgcaatcg tcggcacggc cgtttccgaa cagcggcagc acgctggtga ccggctcgac
     2821 cgtctgcacg acgacgaacg gctcgacgtc gaccaactgc tactgcccga acacggcgcc
     2881 gctggtctgc ggtgccggga tgctcgatgc gcatgccgcc gtcctcgccg cgctgggcgt
     2941 tcaggcgcgc atcacggtcg acacggcaac gccgaccgcg ggccagcccg tctcgctcag
     3001 ctcgaactcg ctcgtcaacg gcaaccagac gatcgccagc tacgcgtgga cgatcacgag
     3061 tgccggcggt acgggcgcga cgatcacggg tgcggccaat gcctcgtccg tcaccgtcac
     3121 gccgacgggc gccggcacgt tcacggtcca gctggtcgtg accgacgccc tcggcgtcac
     3181 gagcacggcg agcacgtccg tggccgtcgc cgcgacatcg tcgggcggtg gcccgaccac
     3241 aggaccgtcg tcgaccggct cgtcgtcggg cggcggtggc gccatcggtg ccggctggct
     3301 gctcttgctg ctggcggccg tcgcggcgct cgccgcggaa gaacggctgc aacgccggcg
     3361 acgggcgacg atcgctagtg tgccggcgac gcgcaggggc tgaaaccctg tcctgacgtg
     3421 ccgagtcgca gcgcggcgag ccgggttgcg agcgccgggt cggccgacgc gacgcggtag
     3481 gtgtgctgga cgccggcggt tccggcgttg gcgatgtcgg tccagctgcc ggcaggcagc
     3541 gtcgcctgca gcgtggcctc ggccgatgcc gcgtccgccg acgcgacggg gccggcctcg
     3601 aagcagccgg ctcgcgcgat gctggccggc gccgaggccc gcggcatcgg catcagggtg
     3661 atgttgccgg gccggatctg gctggcgacc ctctctggct cgcggtcgcc gatcgagctg
     3721 agcccgaaca tgccgtcgaa ccagccgcgc gtgaagccga agaacagcag gttggcgacg
     3781 accagcagca aagtgatcag cctcagcacg atgccctcga cgtctttcct gtcgtgttgg
     3841 cctcggtcgt gcgttgagtt ggtacgggta ccgatgtcga tgtcgatgtc gatgccgatc
     3901 tcgccaagat cgaaagcgag atccaaccac gaatgaagat ccagatggaa ttacaagaaa
     3961 gatggatcat gcaaggttag acatgaatat ctcaaattca ccagtcagga cgtccatctc
     4021 tttttttggc aaaagatttt tgtatgtatc ttcataatca caagaggaaa aaagcggaaa
     4081 aatggcactg aatgtcgac
//
LOCUS       DASRWS010000648         3847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1718581, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000648 DASRWS010000000
VERSION     DASRWS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718581"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3847)
                     /locus_tag="VFF72_12385"
     CDS             complement(<1..>3847)
                     /locus_tag="VFF72_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZV94008.1"
                     /translation="PRLVSALALVHQRFSTNTFPEWPLAHPYRMVAHNGEINTVKGNF
                     NWMRAREGVMKSPVLGDDLKKLYPITFEGQSDTATFDNALELLTMSGYPLAHAVMMMI
                     PEAWEQHPGMDPRRRAFYEYHAAMMEPWDGPAAMVFTDGRQIGATLDRNGLRPARYIV
                     TDDGLVVMASETGVLPIAEHRIVKKWRLQLGKMFLIDFEQGRIVDDEELKSQFASAKP
                     YRQWVDGVRIRLETLPVTTAAPVFTESLLDRQQAFGYTQEDLKFLISPMAQAGEEGIG
                     SMGNDSPLAVLSDRNKPLYNYFKQLFAQVTNPPIDPIREAIVMSLVSFIGPKPNLLDI
                     NAVNPPMRLEVAQPILGFADMARLRAIQAHTSAKFKSYELDITYPLDWGAEAIEAQLA
                     SLCAESVDAIKSGHNILIISDRKVDRTHVALPAPLALSAVHQHLVREGLRTTAGLVVE
                     TGSAREVHHFAVLAGYGAEAVHPYLALETLADLHKDLPAELATDKAVANYVKAIGKGL
                     SKIMSKMGVSTYMSYCGAQLFEAIGLEKALVDKYFRGTASQIEGIGVFVVAEEAIRMH
                     RAAFGEDAVLETMLDAGGEYAWRVRGEEHMWTPDAIAKLQHGVRANRFETYEEYARLI
                     NDQSRRHMTLRGLFEFVVDPARAIPLDHVEPAAEIVKRFSTGAMSLGSISTEAHATLA
                     VAMNRIGGKSNTGEGGEDPARYRAEMKGETIAEGTHFGDIIGKGDIVVDFALKAGDSL
                     RSRIKQVASGRFGVTTEYLMSADQLQIKMAQGAKPGEGGQLPGGKVSDYIGRLRYSVP
                     GVGLISPPPHHDIYSIEDLAQLIHDLKNANQQASISVKLVSEVGVGTVAAGVAKAKAD
                     HVVIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRGRIRVQADGQMKTGR
                     DVVIGALLGADEFGFATAPLVAEGCIMMRKCHLNTCPVGVATQDPLLRAKFAGRPEHV
                     VNYFFFVAEEARRIMARLGVRTFDELIGRVDLLDTKKGVEHWKAQGLDFGRVLHAPTA
                     PATVARRHVESQDHGLERALDVRLIEKCRPAIERAEKVQLLEEARNVNRTVGAMLSGE
                     LIRRRPEGLPDNTIFIQMEGTGGQSFGAFLAKGITIYLIGDANDYTGKGMSGGRLAIR
                     PSIDFRGEATENIIVGNTVLYGATSGEAFFRGVAGERFAVRLSGATAVVEGTGDHGCE
                     YMTGGTVVVLGKTGRNFAAGMSGGVAYVYDDEGDFATRCNTSMVSLGPVVTSAEQEKA
                     GERGVWHREQTDEAQLKALV"
BASE COUNT          610 a         1274 c         1268 g          695 t
ORIGIN      
        1 caccagcgcc ttgagttgcg cttcgtcggt ctgctcgcgg tgccagaccc cgcgttcgcc
       61 ggccttttcc tgttcggccg acgtgacgac cggaccgagc gagaccatcg aggtgttgca
      121 gcgagtggcg aaatcaccct cgtcgtcgta gacgtaggcc acgccgccgc tcattcccgc
      181 cgcgaaattg cgcccggtct tgccgaggac gacgacggtc ccgccggtca tgtattcgca
      241 gccatggtcg ccggtccctt cgacgactgc ggtcgctccg gacagtcgca ccgcgaagcg
      301 ctcgccggcg acgcctcgaa agaacgcctc gccgctggtt gcgccataga ggacggtgtt
      361 gccgacgatg atgttctcgg tggcctcacc gcggaagtcg atgctcggcc ggatcgccag
      421 ccggccgccg ctcatgccct tgccggtgta gtcgttggcg tcgccgatca ggtagatcgt
      481 gatgcccttg gcgaggaagg cgccgaagct ctggccaccg gtgccttcca tctggatgaa
      541 gatcgtgttg tcgggcagac cctcgggacg acgccggatc aactcgcctg agagcatcgc
      601 gccgacggtg cgattgacgt tgcgtgcttc ctcgagaagc tgcaccttct ctgcccgctc
      661 gatggccggc cggcacttct cgatcaggcg aacgtcgagg gcgcgctcca gcccgtggtc
      721 ctgcgactcg acatgtcgtc tcgcgaccgt cgccggcgcc gtcggcgcgt gcaggacgcg
      781 cccgaaatcg aggccctgcg ccttccagtg ctcgacgccc ttcttcgtgt cgagaagatc
      841 gacccggccg atcaattcgt cgaaggttcg aacaccgagc ctggccatga tccggcgagc
      901 ctcctcggcc acgaagaaga agtagttgac gacatgctcc ggcctgcccg cgaacttcgc
      961 gcgcaacagc ggatcctgcg tcgccacgcc caccgggcag gtgttgaggt ggcacttcct
     1021 catcatgatg cagccttcgg caaccagcgg cgcggtggcg aagccgaatt cgtcggcacc
     1081 cagcagcgcg ccgatgacga cgtcgcggcc ggtcttcatc tggccgtcgg cctggacgcg
     1141 aatccgcccg cgcagccggt tgagcaccag tgtctgctgc gtttccgcaa gacccagctc
     1201 ccacggcgtg ccggcatgct tgatcgaact ccagggcgac gcgcccgtgc cgccgtcgtg
     1261 gccggcgatg acgacgtggt ccgccttcgc cttggcgacg ccggcggcga cggtgccgac
     1321 gccgacctcc gacacgagct tgacactgat gcttgcctgc tggttggcat tcttcaggtc
     1381 gtggatgagc tgcgcgaggt cttcgatcga atagatgtcg tgatgcggcg gcggcgagat
     1441 gagaccgacg cccggcaccg aatagcgcag gcggccgatg tagtcgctga ccttgccgcc
     1501 aggcagctga ccgccctcgc ccggcttggc gccttgcgcc atcttgatct gcagctgatc
     1561 ggcagacatg aggtattcgg tcgtgacacc gaaccgcccc gaggcgacct gcttgatccg
     1621 cgagcgcagc gaatcgccgg ccttcaacgc gaagtcgacg acgatatcgc ccttgccgat
     1681 gatgtcgccg aaatgcgtgc cttcggcgat cgtctcaccc ttcatctcgg cccgataacg
     1741 cgccggatcc tcgccaccct cgccggtgtt ggacttgccg ccgatgcggt tcatggcgac
     1801 ggccagcgtg gcgtgggctt cggtcgagat cgatccgagc gacatcgcac cggtcgagaa
     1861 gcgcttgacg atctcggccg ccggctcgac gtggtcgagc ggaatcgcgc gagcaggatc
     1921 gacgacgaac tcgaacaggc cgcgcagcgt catgtgccgg cggctctggt cgttgatcag
     1981 ccgggcatat tcctcgtagg tctcgaatcg attggcgcgt acgccatgct gcagcttggc
     2041 gatggcgtcg ggggtccaca tgtgctcctc accgcgtacc cgccaggcgt attcgccgcc
     2101 ggcgtcgagc atggtctcga gaaccgcgtc ctcgccgaac gcggcgcggt gcatgcggat
     2161 cgcctcttcg gcaacgacga agacgccgat gccttcgatc tggctcgccg tgccacggaa
     2221 atacttgtcg acaagcgcct tctcgaggcc gatcgcttcg aacagctggg cgccgcaata
     2281 cgacatgtac gtcgacacgc ccatcttcga catgatcttg gacaacccct tgccgatcgc
     2341 cttgacgtag ttggcgactg ccttgtcggt cgccagctcg gcaggaaggt ccttgtgcag
     2401 gtcggcgaga gtctcgagcg cgagatacgg atggaccgcc tcggcgccat agccggcaag
     2461 gaccgcgaag tgatgcacct cgcgggccga acccgtctcg acgaccagcc cggcggtcgt
     2521 gcgcaggcct tcgcgaacca gatgctggtg caccgccgag agcgccagtg gcgccggcag
     2581 ggcgacgtgc gtgcgatcga ccttccggtc gctgatgatg aggatgttgt ggccgctctt
     2641 gatcgcatcg acgctctcgg cgcagagcga cgccagctgc gcctcgatgg cctcggcgcc
     2701 ccagtcgagc gggtaggtga tgtcgagctc gtagctcttg aacttggcac tcgtgtgcgc
     2761 ctggatcgcg cgcagccggg ccatgtcggc gaagccgagg atcggctgcg ccacctcgag
     2821 ccgcatcggc ggattgacgg cgttgatgtc gagcaggttg ggcttggggc cgatgaacga
     2881 caccagcgac atgacgatgg cctcgcggat cggatcgatc ggcggattcg tgacctgcgc
     2941 gaacaactgc ttgaagtagt tgtagagcgg cttgttcctg tcggagagca cggcgagcgg
     3001 cgaatcgttg cccatcgagc cgatgccttc ctcccccgcc tgggccatcg ggctgatcag
     3061 gaacttcagg tcttcctgcg tgtagccgaa cgcctgctgg cgatcgagca ggctctcggt
     3121 gaagaccggc gccgctgtcg tgaccgggag cgtttccagg cggatgcgca cgccgtcgac
     3181 ccattgccga tacggcttgg ccgaggcgaa ctggctcttc agctcctcgt cgtcgacgat
     3241 ccggccctgc tcgaagtcga tcaggaacat cttgcccagc tgcaggcgcc acttcttgac
     3301 gatgcggtgc tcggcgatcg gcaggacgcc ggtctcggaa gccatcacca ccaggccgtc
     3361 gtcggtgacg atgtagcgcg ccggtcgcag gccgttgcga tcgagcgtcg cgccgatctg
     3421 ccggccgtcg gtgaagacca tcgccgccgg gccgtcccac ggctccatca tcgccgcgtg
     3481 gtactcgtag aaggcacgcc ggcgcggatc catgccggga tgctgctccc acgcctcggg
     3541 gatcatcatc atcaccgcgt gggccagcgg gtagccgctc atcgtcagca gctcgagggc
     3601 gttgtcgaag gtcgcggtgt cgctctgccc ctcgaaagtg atcggataga gcttcttcag
     3661 gtcgtcgccc agtacgggcg acttcatcac gccttcgcgc gcgcgcatcc agttgaagtt
     3721 gcccttgacg gtgttgatct cgccgttgtg cgcgaccatg cggtacggat gggcgagagg
     3781 ccactcggga aacgtgttgg tcgagaagcg ctggtgaacg agcgccaggg ccgaaacgag
     3841 gcgcgga
//
LOCUS       DASRWS010000649         4425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_260984, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000649 DASRWS010000000
VERSION     DASRWS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260984"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VFF72_12390"
     CDS             complement(<1..175)
                     /locus_tag="VFF72_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZV94009.1"
                     /translation="MRRPKPPAPVTGSADDVEAEFYEALQHGDIDRLMALWSDDDDIA
                     CVHPGGPRLVGAPA"
     gene            234..872
                     /locus_tag="VFF72_12395"
     CDS             234..872
                     /locus_tag="VFF72_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZV94010.1"
                     /translation="MVKPATDWTERSLDLARLLGRAGLGDRAAFATLYERTSPHLFAV
                     VLRINRDRAQAEDILQEVYVNVWRAAGTFDAARSQPLTWLTSIARNRAIDSLRRGQTQ
                     PQMQPTSGNPAATDAEDQSVYDTVADASPGPLDLLSRASDARALSECMEQLSSLQRQS
                     VALAFFHGLSHAEVASQLRQPLGTVKSWVRRALLVLKGCLQGAVEADGRSGD"
     gene            874..1569
                     /locus_tag="VFF72_12400"
     CDS             874..1569
                     /locus_tag="VFF72_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZV94011.1"
                     /translation="MDYSRTDLADRLASQYVAGTLRGAARRRFEGLLPAHPALRDAKR
                     AWEDRLMPLTLAIAPVQPSGNVWRRISERIGNGDAKAAAPGLWHRLSFWRGFASLASV
                     ATIALAVLLANPRPTAPPVVVVLAATAAATGNVPPTSIVASISGDGSSLVTRAIAPVA
                     LRPDRSLELWAVPTAGAPRSLGLLPGGSGTVALRGKVLAGADTLAVTVEPAGGSPTGK
                     PTGPIVYAGKFTL"
     gene            complement(1559..2641)
                     /gene="waaF"
                     /locus_tag="VFF72_12405"
     CDS             complement(1559..2641)
                     /gene="waaF"
                     /locus_tag="VFF72_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZV94012.1"
                     /translation="MTRPPAALASRVLVVAPQWIGDAVMAAPLIAALARGGARITVAA
                     LPWVAPVCRAMSGVAEIVELPFAHGRLDATLRLRVARSLRGRFDAACVLPNSIKSALL
                     PWLARIPLRIGYQGEGRPILLNRRLPNPAGRPPMVAFYGALAGETFDPEATPRLQLPE
                     ATVDAVLAATGLQRGSYRVFAPGAEYGPAKRWPAGHYAALARSLHALDGRVVVLLGSK
                     GEADLCQAIAVEAPGACRVLAGKTTLLEAMALIAASRGLASNDSGLMHVAAGFGVPQV
                     AVFGSTSPLHTPPLNPRARVLWLKEELGLDCMPCFDRQCRYGHTLCLVGVAPDRVEVA
                     LREAEASTRLGVGVDPASGPEASLRE"
     gene            complement(2656..2898)
                     /locus_tag="VFF72_12410"
     CDS             complement(2656..2898)
                     /locus_tag="VFF72_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZV94013.1"
                     /translation="MADEGVTAMNSGDPRKSAVVELSASELEGPGVVFCPNPKMPLWS
                     NHPRVFIDVAQTGSGKCPYCGTEYRLKAGEQVRSSH"
     gene            complement(2880..3803)
                     /locus_tag="VFF72_12415"
     CDS             complement(2880..3803)
                     /locus_tag="VFF72_12415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZV94014.1"
                     /translation="MSMADRDGKIWMDGKLVEWRDAKIHVLTHTLHYGCGAFEGVRAY
                     DTVNGTAIFRLYEHTQRLFNSAKILRMTIPYAFDDVVEAQRTVVRENKLESCYLRPLA
                     WIGSEKLGVSPKGNTIHLMIAAWPWGAYLGEEGLKRGIRVKTSSYTRHHVNITMTQAK
                     TVSNYTNSILANMEATEDGYDEALLLDASGFVSEGAGENLFMVKKGVVYTPDLSAGAL
                     DGITRNTILAICGDLGLKLVEKRITRDEVYICDEAFFTGTAAEVTPIRELDRLSIGSG
                     SRGPVTEKIQAAFFDIVNGRNPKYAEWLTKV"
     gene            complement(3874..4338)
                     /locus_tag="VFF72_12420"
     CDS             complement(3874..4338)
                     /locus_tag="VFF72_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94015.1"
                     /translation="MRPAVAWMLAAGFAFVAHSLYGWRGVALAASVIAFGLLLQFNRT
                     IRVMRVAAENPVARISNAVMFQAGLREGMSMLQVVRTTRTLGRRVDGGVDDWLWSDAS
                     GDAVRLHFERGRLTKWRLERAVPPADASVPGSASETSAEASPASSPSPSPDR"
BASE COUNT          671 a         1565 c         1523 g          666 t
ORIGIN      
        1 tcgccggcgc gccgacgagc ctcgggccgc cgggatgcac gcaggcgatg tcgtcgtcgt
       61 ccgaccagag cgccatcagc cgatcgatgt cgccgtgctg cagcgcttcg tagaattcgg
      121 cttcgacgtc gtcggccgac ccggtcacag gggcgggcgg cttggggcgg cgcatcggtt
      181 agtctcctgc gctgattgta ggaaggcgcg cccgccttcg agacctcgct ttcatggtca
      241 aacccgccac cgactggacc gagcgcagcc tcgacctcgc ccgcctcctc ggccgcgccg
      301 ggctcggcga tcgcgcggca tttgccacgc tctacgagcg aacgagcccg catctgttcg
      361 ctgtcgttct tcgtatcaac cgggaccgag cccaggccga ggacattctc caggaggtct
      421 acgtcaacgt ctggcgggcg gcgggcacgt tcgacgcggc acgcagtcag cccttgacgt
      481 ggctgacgag catcgctcga aatcgcgcca tcgacagcct gcgtcgcggc cagacgcagc
      541 cgcagatgca acccacgagc ggcaatccgg ccgccaccga cgcggaggat cagagcgtgt
      601 acgacaccgt tgccgacgcc agcccgggtc cgctcgacct gttgagcagg gcgtcggacg
      661 cgcgcgccct cagcgagtgc atggagcagc tttcgtcgct gcagcggcaa agcgtcgccc
      721 tcgccttctt tcacggcctc agccacgccg aggtcgcctc gcaattgcgc cagccgctgg
      781 gcacggtgaa atcgtgggtg cggcgggcct tgctcgtgct gaaaggctgc ctgcaaggcg
      841 ccgtcgaggc cgacggccgc agcggcgatt gacatggact acagccgcac cgatctcgcc
      901 gaccgactcg cctcgcagta cgtggccggc acgttgcgtg gcgcggcgcg acgtcgcttc
      961 gaggggctgc tgcccgcgca tccggcgctg cgcgacgcga agcgcgcctg ggaagaccgg
     1021 ctgatgccgc tgaccctggc gatcgcgccg gtccagccgt cgggcaacgt ctggagacgc
     1081 atctcggaaa ggataggcaa cggcgatgcg aaggccgccg cgccgggcct ctggcatcgc
     1141 ctgtcgttct ggcgcggctt cgcctcgctg gcgagcgtcg cgacgatcgc tctggccgtg
     1201 ctcctggcca acccgcggcc gacggcaccc ccggtcgtcg tcgtcctcgc cgccaccgcg
     1261 gccgccaccg gcaacgtgcc gccgacctcc atcgtcgcca gcatcagcgg cgacggctcc
     1321 agcctggtga cgcgagcgat cgcgccggtg gcgctgcgtc cggatcggtc gctcgaactg
     1381 tgggcggtcc ccaccgcagg cgcgccgcgt tcgctgggac tgctgcccgg cggcagcggc
     1441 accgtcgcgc tgcgcggcaa ggtcttggcc ggggccgaca ccctggcggt gacggtggag
     1501 ccggccggcg gatcgcctac cgggaagccg accgggccga tcgtctacgc cggcaagttc
     1561 actctctgag cgaagcctcc gggccggacg ccgggtcgac accgacgccg agtcgtgtcg
     1621 acgcctcggc ttcgcgcagc gccacctcga cacgatcggg cgccacgccg accaggcaga
     1681 gtgtgtggcc atagcggcac tgtcggtcga agcacggcat gcagtcgaga cccagctctt
     1741 ccttcagcca cagcacgcgg gcgcgcggat tgagcggcgg cgtgtgcagc gggctggtcg
     1801 agccgaacac ggcgacctgg ggaacaccga acccggcggc gacgtgcatc aggcccgagt
     1861 cgttgctcgc cagtccgcgc gacgcggcga tcagggccat cgcctcgagg agcgtggtct
     1921 tgcctgccag gacgcgacag gcaccgggcg cctcgacggc gatcgcctgg cacaggtcgg
     1981 cctcgccttt cgagcccagc aacacgacga cgcgaccatc gagcgcgtgc aacgagcgcg
     2041 ccagggcggc gtagtgcccc gccggccagc gcttggccgg accgtactcc gcgcccggcg
     2101 cgaagacgcg atacgaaccg cgttgcagtc cggtcgccgc gagcaccgca tcgacggtcg
     2161 cctcgggcaa ctgcagtcgc ggtgtcgcct ccggatcgaa ggtctctccg gcgagggcgc
     2221 catagaacgc caccatcggg gggcggccag cgggattggg caggcgccga ttgagcagga
     2281 tcggccggcc ttcgccctga tagccgatac gcagggggat ccgcgccaac cagggcagca
     2341 gcgccgactt gatcgaattc ggcaggacgc aggcggcgtc gaagcggccg cgcagcgacc
     2401 gcgcgactcg caggcgcagg gtcgcgtcga gccggccgtg agcgaacggc aactcgacga
     2461 tctcggcgac gccggacatg gcgcgacaga ccggagccac ccaaggcaac gcggccacgg
     2521 tgatccgcgc gccgccccga gccagcgccg cgatcagcgg cgcggccatc actgcgtcgc
     2581 cgatccactg cggcgcgaca acgagaacgc gtgacgccag ggccgcgggc ggacgcgtca
     2641 cggtgaccgg cgcgatcagt gcgacgaacg aacctgttcg cccgccttca gccggtactc
     2701 ggtgccgcag tacgggcact tgcccgagcc ggtctgcgcg acatcgatga agacgcgcgg
     2761 atggttgctc cacagcggca tcttcgggtt cgggcagaag acgacgccgg gaccctccag
     2821 ctcggaggcg gacaactcga ccaccgcgct cttcctcgga tcgccgctgt tcatcgccgt
     2881 cacaccttcg tcagccattc ggcgtacttc ggattgcggc cgttgacgat gtcgaagaag
     2941 gcggcctgga tcttctcggt gaccgggccg cgcgagccgc tgccgatcga caggcgatcg
     3001 agctcgcgga tcggcgtcac ctcggcggcg gtgccggtga agaacgcctc gtcgcagatg
     3061 tagacctcgt cgcgggtgat tcgtttctcg accagcttca ggccgaggtc gccgcagatc
     3121 gcgaggatcg tgttgcgggt gatgccgtcg agcgcgcccg ccgacaggtc gggcgtatag
     3181 acgacgccct tcttgaccat gaagaggttc tcgcccgcgc cttcgctgac gaagccgctc
     3241 gcgtcgagca gcagcgcctc gtcgtagccg tcttcggtgg cttccatgtt ggcgaggatc
     3301 gagttggtgt agttcgagac cgtcttggcc tgcgtcatcg tgatgttgac gtgatggcgc
     3361 gtgtagctcg aggtcttgac ccggatgccg cgcttgaggc cttcttcgcc gaggtaggcg
     3421 ccccacggcc aggcggcgat catcaggtgg atggtgttgc ccttgggcga gacgccgagc
     3481 ttctcggagc cgatccaggc aagcggccgc aggtagcacg actcgagctt gttctcgcgc
     3541 accaccgtgc gctgcgcctc gaccacgtcg tcgaacgcgt acgggatcgt catgcgcagg
     3601 atcttggcgc tgttgaagag gcgctgcgtg tgttcgtaga ggcgaaagat cgccgtgccg
     3661 ttgaccgtgt cgtaggcgcg cacgccctcg aacgcgccgc atccgtagtg cagcgtgtgc
     3721 gtgaggacgt ggatcttcgc gtcgcgccac tcgacgagct tgccgtccat ccagatcttt
     3781 ccgtcgcgat cggccatcga catgacgcgc tccactgcaa ttcttccggg gcgtcgattc
     3841 taggtcgctg cgcccgtgtc gatcgccggc ggctcaccga tcaggcgacg gcgacggcga
     3901 tgacgcgggc gacgcttcag ccgatgtctc ggatgccgac ccgggcaccg atgcgtcggc
     3961 cggcggcact gcccgctcca ggcgccactt cgtcagccgg ccgcgctcga agtgcagccg
     4021 aacggcgtcg ccggaggcgt cgctccacag ccagtcgtcg acgccgccat cgacgcgtcg
     4081 cccgagcgtt cgcgtcgtcc tgacgacctg cagcatcgac atcccttcgc gcagcccggc
     4141 ctggaacatc accgcattcg agatgcgcgc caccgggttc tccgcggcga cgcgcatcac
     4201 gcggatcgtg cggttgaatt gcagcagcag cccgaaggcg atgacgctcg ccgccagcgc
     4261 cacgccgcgc cagccgtaga gcgaatgcgc cacgaaggca aaccccgccg cgagcatcca
     4321 ggcgactgcc ggcctcatcg ccttggccgc cggccgctca aagttcgcgg atccggtcga
     4381 tcgccgcctc gatgcgatcg acggcgtgga tcgtcagccc ttcga
//
LOCUS       DASRWS010000650         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2281651, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000650 DASRWS010000000
VERSION     DASRWS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281651"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFF72_12425"
     CDS             complement(<1..325)
                     /locus_tag="VFF72_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HZV94016.1"
                     /translation="MIRRFASLALIASAAAAFSGGAMAVPVGVSIDIGPPPPRVEVVP
                     APRPGYLWTPGYWDWRHGRHYWVGGTWVRARPGYIYTRPEWVNVGGHWRLRHGGWARG
                     PYGDRD"
     gene            596..>2071
                     /locus_tag="VFF72_12430"
     CDS             596..>2071
                     /locus_tag="VFF72_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZV94017.1"
                     /translation="MNDDPYLWLENVDGAKTLDWARARNADTRRALESRPGYDATYKA
                     LLSIYDSKDRIAQVNRRGPWFYNFWQDDRHERGILRRTTLDGYRKADVPWEIVLDLDA
                     LDRAEHENWTWKGMTCLGPSYRRCLVSLSRGGADAVVVREFDSVDKRFVDDGFTLPEA
                     KSDVAWIDADSIYVGTDFGPGSLTDSGYPRIVKRWRRGTPLASARTVFEGRPSDVSVG
                     VTIDRTPGFERTLFTRSVTFWNNRRFLLAGDALVPVDVPDDADFSFLRDLAIVRLRSD
                     WNVGGHRYASGSLLASNADAYLAGKREFSVLFEPTKTRSLAGWTYTRDAVLVDVLDNV
                     ASRIEELDREGNAFHRRDVKTPFPGSIGIAPLYDPLVDGVPAAAGRLAVPATTDGSLA
                     NRYWFTYVDFLTADSLALATAGSDAREVVKTRPPLFDADGMHVDQFFAHSKDGTRVPY
                     FVVWPKGRGANEAGHSDTPTLLYGYGGFEISMAPFYSGGYGA"
BASE COUNT          309 a          748 c          655 g          359 t
ORIGIN      
        1 ggtcgcgatc accgtacggt ccgcgcgccc atccgccgtg cctcaagcgc cagtggccgc
       61 cgacgttcac ccattcggga cgcgtgtaga tgtagcccgg ccgcgcccgc acccacgtgc
      121 cgccgaccca gtagtgccgg ccgtgccgcc agtcccagta gccgggcgtc cagagatagc
      181 cgggccgtgg cgccggcacg acttcgaccc ggggcggtgg cggcccgatg tcgatggaca
      241 cgccgacggg cacggccatc gcgccgccac tgaaggcggc tgcggccgag gcgatgaggg
      301 ccagcgaggc gaatcttctg atcatggttg ttctccgtgg caatgcgtga agcttcaata
      361 tcggcagaat cgcgtcctcc ttgcatcgga ggtcgtcgtg ccgtcccgtc agcatcgtcc
      421 tgcacgaggc tttcccaagc gtgacggttc gtttccggat gtcttgcgtg cactatttgt
      481 tgttgccgtg aatcccttgt cccttttgcg ccgcagccga cgcaccggcg ccggcgccgc
      541 ccttctcgtc tcgctcctcg cattcctttg cccaacgatg gctcttgccg acacgatgaa
      601 cgacgatccc tatctctggc tcgagaacgt cgacggcgcc aagacgctcg attgggcgag
      661 ggcccgcaac gccgacaccc ggcgcgcgct cgagtcgaga cccggctatg acgcgaccta
      721 caaggcgctc ctgtccatct acgattcgaa ggaccgcatc gcgcaggtga accggcgcgg
      781 cccctggttc tacaacttct ggcaggacga tcgccatgag cgcggcatcc ttcgccgcac
      841 gacgctcgac ggctaccgca aggccgacgt cccctgggag atcgtcctcg acctcgacgc
      901 gctcgaccgg gccgagcacg agaactggac ctggaaaggc atgacctgcc tcggcccttc
      961 gtaccgccgc tgcctcgttt cgctctcgcg cggcggcgcc gacgccgtcg tcgtccgcga
     1021 attcgacagc gtcgacaagc gcttcgtcga tgacggcttc acattgccgg aggcgaagtc
     1081 cgacgtcgcc tggatcgacg ccgactcgat ctacgtcggc accgatttcg gccccggctc
     1141 gctgaccgat tcgggctacc cgcgcatcgt caagcgctgg cgacgaggca cgccgctggc
     1201 gtcggccagg accgtgttcg aaggccggcc gagcgacgtc tcggtgggcg tgaccatcga
     1261 ccgcacgccg ggcttcgagc gcacgctgtt cactcggtcg gtcacgttct ggaacaacag
     1321 gcgcttcttg ctcgccggtg acgcgctcgt cccggtcgac gttcccgacg acgccgactt
     1381 cagcttcctt cgcgatctcg cgatcgtccg gttgcgcagc gactggaatg tcggcggcca
     1441 ccgatacgcc tcgggctcgc tgctcgcgtc gaatgccgat gcgtacctcg ccggcaagcg
     1501 cgagttctcg gtcctcttcg agccgacgaa gacccgatcg ctcgccggct ggacctacac
     1561 gcgcgacgcc gtgctggtcg acgtgctcga caacgtcgcc agccgcatcg aggaactcga
     1621 ccgcgaaggc aacgcttttc atcgtcgcga cgtgaagacg ccgtttccgg gatcgatcgg
     1681 catcgcgccg ctctacgacc cgctcgtcga cggcgtgccc gccgccgccg gcaggctggc
     1741 ggtgccggcc acgaccgacg gcagcctcgc caaccgctac tggttcacgt atgtcgattt
     1801 cctgaccgcc gattcgctgg cgctggcgac cgccggcagc gatgcccgcg aggtcgtgaa
     1861 gacgcggcca cctctgttcg acgccgacgg catgcacgtc gaccagttct tcgcgcactc
     1921 gaaggacggc acgcgcgtgc cgtatttcgt ggtctggccg aagggccgcg gcgcgaacga
     1981 ggccggccac tccgacacgc cgaccctgct ctacggctac ggcggcttcg agatctcgat
     2041 ggcgccgttc tattccggcg gctatggcgc g
//
LOCUS       DASRWS010000651         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_262146, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000651 DASRWS010000000
VERSION     DASRWS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262146"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFF72_12435"
     CDS             <1..1047
                     /locus_tag="VFF72_12435"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZV94018.1"
                     /translation="SKGGIRYHPDVTLDEVKSLAMWMTWKCALMGIPFGGAKGGVVVN
                     PKRLSRSELERMTRRYTSEIGIIIGPQRDIPAPDVNTNGQVMAWMMDTYSMNTGSNAT
                     GVVTGKPIHLGGSLGRVKATGRGVFVTGREAARRLGLNLDGARVAVQGMGNVGASAAE
                     LFVQAGAKLVAMQDHTGTRVDPGGFDMASTMGAITHEAGIANVKGGERIDNEDFWGVD
                     CDILVPAALEGQITPERAQRIKARLILEGANGPTLPEADDVLAERGILVVPDVICNAG
                     GVTVSYFEWVQDFSSFFWSEDEINVRLDKIMVDALKKIWDTADRHKISLRTATFAVAC
                     ERILIAREERGLYP"
     gene            complement(1157..1624)
                     /locus_tag="VFF72_12440"
     CDS             complement(1157..1624)
                     /locus_tag="VFF72_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZV94019.1"
                     /translation="MSSRLDAIDRKILQALQDDGRISNVDLAAKIHLSAPQCFRRVRS
                     LEERRVLRGYRADVDPQALGFEVTAYVSVSIGGGDFGRVREIEAEMRAFPQILECFSV
                     SGEDDYLLKVVAHDLKSLSNFLTDYLMQVPGIDDVRSMICLEEIKPASPLPVT"
     gene            1781..>2119
                     /locus_tag="VFF72_12445"
     CDS             1781..>2119
                     /locus_tag="VFF72_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZV94020.1"
                     /translation="MATPGRAQETPAELERRYGAANYHPLPVTLQRGAGVWLFDDQGR
                     RYLDMMSAYSAVSFGHGHPTLLRALSEQAERVAVTSRAFHTDRLGPFLRAVCETTGID
                     KALPMNSGAEA"
BASE COUNT          383 a          703 c          701 g          332 t
ORIGIN      
        1 tcgaagggcg gcatccgcta tcacccggac gtcacgctcg acgaggtcaa gtcgcttgcg
       61 atgtggatga cgtggaagtg cgcgctgatg ggcatcccgt tcggcggtgc gaagggcggc
      121 gtcgtcgtca acccgaagcg cctctcgcgc agcgagctcg agcgcatgac gcgccgctac
      181 acgagcgaga tcggcatcat catcggaccg cagcgcgaca ttccggcgcc cgacgtgaac
      241 accaacggcc aggtgatggc ctggatgatg gacacgtact cgatgaacac cggctcgaat
      301 gcgaccggcg tcgtcaccgg caagcccatc catctcggcg gttcgctcgg ccgggtcaag
      361 gcgaccggcc gcggagtctt cgtcaccggc cgcgaggcgg cgcgtcgtct cggattgaat
      421 ctcgacggcg cccgggtggc ggtgcaggga atgggcaacg tcggcgcctc agccgccgag
      481 ctcttcgtgc aggccggcgc caaactcgtc gcgatgcagg accataccgg cacgcgcgtc
      541 gatccgggcg gcttcgacat ggccagcacg atgggcgcga tcacgcacga ggcgggcatc
      601 gccaacgtga agggcggcga gcgcatcgac aacgaggact tctggggagt cgactgcgac
      661 atcctcgtcc cggcggcgct cgaaggccag atcacgcccg agcgggccca gcgcatcaag
      721 gcccggctca tcctcgaggg cgccaacggc ccgaccctgc ccgaggccga cgacgtgctc
      781 gccgagcgtg gcatcctggt cgtccccgac gtcatctgca acgccggtgg cgtcaccgtc
      841 agctacttcg agtgggtgca ggatttctcg agcttcttct ggagcgagga cgagatcaat
      901 gtccgcctcg acaagatcat ggtcgatgca ctgaagaaga tctgggacac cgccgaccgg
      961 cacaagatca gcctgcgcac cgccacgttc gcggtcgctt gcgaacgcat cctgatcgcc
     1021 cgcgaggaga gagggctgta tccctgagcc agtccaggga tacaaccctc ttcgagggtc
     1081 agggctgctc ccgccccaac ccctccctcc gggagggagg ggccgacccc gccggggtcg
     1141 ctcacatctc ggccgctcag gtgacgggga gcggcgatgc gggcttgatc tcctcgaggc
     1201 agatcatcga tcgcacgtcg tcgatgcccg gcacctgcat caggtagtcg gtgaggaagt
     1261 tgctcaggct cttcaggtcg tgcgccacca ccttgagcag gtagtcgtct tcgcccgaga
     1321 cgctgaagca ttcgaggatc tgcgggaagg cgcgcatctc ggcttcgatc tcgcgcacgc
     1381 gcccgaaatc tcctccgccg atgctgacgc tcacatacgc cgtgacttcg aagccgagag
     1441 cttgcggatc cacgtcggcg cgatagccgc ggagcacccg acgctcctcg agcgaacgaa
     1501 cgcggcgaaa gcactgcggc gccgagagat ggatcttggc cgccaggtcg acgttggaga
     1561 tccgcccgtc gtcctgcagg gcttgaagga tctttcgatc gatcgcatcg aggcgtgagc
     1621 tcatcggccg tactgcgctg gctgcgaaag attattgtgt cagacggcag aaaggcgcaa
     1681 gaaagagaaa gcacatttcg ccggctgcgg cgcaaattgg acatccacga gagataccgg
     1741 agcgcccatg ccagccacca cactcagtcc atcgcgccgt atggccacgc caggcagggc
     1801 ccaggaaacc ccggccgaac tcgagcggcg ctatggcgct gccaactatc acccgctgcc
     1861 ggtgacgctg cagcgtggcg ccggcgtgtg gctgttcgac gaccagggcc gtcgctatct
     1921 cgacatgatg tcggcctatt cggcagtcag cttcggccac ggccacccga ccttgctgcg
     1981 ggccttgtcg gagcaggccg agcgcgtcgc ggtgacgagt cgcgccttcc acaccgaccg
     2041 gctcggaccg ttccttcgcg cggtctgcga aacgacgggg attgacaagg cgttgccgat
     2101 gaacagcggt gccgaggcg
//
LOCUS       DASRWS010000652         5893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_542673, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000652 DASRWS010000000
VERSION     DASRWS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542673"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFF72_12450"
     CDS             <1..1002
                     /locus_tag="VFF72_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZV94021.1"
                     /translation="QMSLLPQSEDAAERGWRGLLGISELTGEFAKRHPAVTAASVIDF
                     MVSDEANPSSIRSCLLAARENARAVRGTLTTEVWETQNQTWLEFQRMVGDDAFQRAPS
                     EAFEWVKFRSHLSRGVTVGTMLQDEAFHFLRIGSFLERADNTARLLDVKFHAAPESAR
                     SGRVGRRPSARSANASSQSQSQSQSQTQSRSQPAVSQAMTTQGEAESPGKDEEFDFYH
                     WSAVLRSVSGFEIYRRVYRNVIRPERVAELLVLRADMPRSLAACVHEVVTNLERVANS
                     RSQDTLRLAGRLAADLRFGRIDEILATGLHAYLTQFLERINTLGAGISRDFLVPMAD"
     gene            1246..1554
                     /locus_tag="VFF72_12455"
     CDS             1246..1554
                     /locus_tag="VFF72_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94022.1"
                     /translation="MPDPKPQELSLVPIESEPPVGDAAPSTRRRGSAVVSLAPSSPSA
                     PRSSERAHRGPGWFDSSWDLQTGCEVREGWPREAGLFEWIESWLGQPGIDVAQSLCDA
                     "
     gene            complement(1612..2706)
                     /gene="mltB"
                     /locus_tag="VFF72_12460"
     CDS             complement(1612..2706)
                     /gene="mltB"
                     /locus_tag="VFF72_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZV94023.1"
                     /translation="MIDPGRRRTFLAAAALLATTAAPALAAKRKARRPRAAVATDDAP
                     DVVTYGGRDDVMQFADAVALRQGLEPAAVRAALAQARFMPAVTRYIMPPPQGAAKNWA
                     AYRARFVEPIRIGAGVSFWRDNEAWLRLAEERYGVPPEIVVGIVGVESIYGRHMGSFR
                     VIDSLATLSFDFPAGRSDRSEFFRGELESWFVLCNNQAIDPLDWQGSYAGAIGMAQFM
                     PSSFNRFAVDLDGDGHVDLRRSAADVIGSVAHYLAQSGWRHGLAVRYPVVPPLDAANL
                     ATLRAPDILPTFTAGQMSALGASLPESAAATDSALALVELQNGADDPTFVAGTTNFYA
                     ITRYNWSSYYAMAVTDLGEAVRDAVEGARS"
     gene            complement(2715..4775)
                     /locus_tag="VFF72_12465"
     CDS             complement(2715..4775)
                     /locus_tag="VFF72_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HZV94024.1"
                     /translation="MSTRAAASAPGWMPLWPGWSRLPREARDTLFLLAVIAWTVLPHA
                     SHLPAWSIALTTVVLLWRGRLALVGGALPPRWVLVVVLAIAVGLTWWSYRTLLGKEPG
                     ITLAVSLMALKTLELRARRDAFVVFFLGFFIILTHFLYSQSVAVAAAMLVSVWGLLTA
                     VVLAHMPVGQPSLRQASGLAARTALLGAPVMALLFVLFPRIGPLWGVPQDGVSTTGLS
                     NSMKMGSIAEVALDDSVAMRLRFDGAPPPPAQRYFRGPVLTRFDGVDWRPLGLPFAPA
                     GAPARRPALTLRGPPIGYEVTLEPLRIATLPLLEATVSVSPVDGYRVVARDDLEWLAD
                     RPIVDRVRFHAVADTHFTLGAPRRPGELEESLDLPPGYNPRTIAWARRFRADPLRRGD
                     SASEFAAAVLRYIGSAGFSYTLSPGDYGRDGIDEFWLDRKEGFCEHFAAAFVVVMRAA
                     GIPSRVVTGYQGTDPTPVDGFWIVRQSSAHAWAEFWQAGAGWVRADPTAAVAPDRIGR
                     SHRLVPPPGLMTEALDSVSPAFVVGLRVGWEAFNNRWNQWVLNYSRGQQLVVLKNLGF
                     QSPGWEDLALLLIGCLSALAVAAAGWAWVDRHRLDPWVRQLDRMKRSLRTIGVAAAPH
                     DPPRALAAQVRDRLGAAGEPLAAALETLEARRYGRDGARRPVRDMTRRFVADARRLRA
                     TIPR"
     gene            complement(4783..5769)
                     /locus_tag="VFF72_12470"
     CDS             complement(4783..5769)
                     /locus_tag="VFF72_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZV94025.1"
                     /translation="MDIAALNPAAFTRGRFRRWWQARVPRTDTQTLTQRNVYILPTRA
                     GWMFGLTLIVLLLASINYQLSLGYVLTFLLAGSGVVSMHVTHATLRGLTLHLRPVDAA
                     FAGEPAVLDVVLSSPGTARFGIGLRMLEAPESTLVWVDVAAGGRASPRVSFVPPRRGL
                     HEVPTLSAETRFPLGLFRAWTIWRPAARLLVYPHPESGAPPLPAARPVPGGPSSSRSS
                     DGGEIEGVRAYRRGDPLKLVAWKKAAQALETGAELVSRDTSVSNRHELWLDWAACGAL
                     AAEDRLSRLAAWTLAATRSGADFGLRLPDVELSPGSGEVQQRHCLEALALWA"
     gene            complement(5769..>5893)
                     /locus_tag="VFF72_12475"
     CDS             complement(5769..>5893)
                     /locus_tag="VFF72_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZV94026.1"
                     /translation="PDDVQYVLPQTIAHRLLPAAGAGRGRVEQVRAMIEAVALP"
BASE COUNT          942 a         2000 c         2145 g          806 t
ORIGIN      
        1 cagatgtcgt tgctgccgca gtcggaggac gccgcggaac gcggctggcg aggcctgctc
       61 ggaatcagcg agctgaccgg cgaattcgcg aagcgtcacc ctgcggtcac ggccgccagc
      121 gtcatcgatt tcatggtgag cgacgaggcc aacccgtcgt cgatccgcag ctgcctgctg
      181 gcggcccggg agaatgcgcg cgccgttcgc ggcacgctga ccaccgaggt gtgggagacg
      241 cagaaccaga cgtggctcga gttccagcgg atggtcggcg acgacgcgtt ccagagggcg
      301 ccgagcgagg ccttcgagtg ggtcaagttc cgctcgcatc tgtcgcgcgg cgtcaccgtc
      361 ggcaccatgc tccaggacga ggcgtttcat ttcctgcgca tcggcagctt tctggagcgc
      421 gccgacaaca ccgcgcgact gctcgacgtc aagttccacg ccgcgcccga atcggcgcgc
      481 agcggccgcg tcggccggcg tccgtcggcg cgttcggcga acgcgtcatc gcaatcgcaa
      541 tcccagtccc agtcgcaaac ccagtcgcgt tcgcaacccg ccgtgtcgca ggcgatgacg
      601 acgcagggcg aggccgaatc gcccggcaag gacgaggagt tcgacttcta tcactggtcg
      661 gcggtgctgc gctcggtctc cggcttcgag atctatcggc gcgtctatcg caacgtgatc
      721 aggccggaaa gagtcgccga gctgctcgtg ctgcgcgccg acatgccgcg ttcgctcgcg
      781 gcatgcgtcc atgaggtcgt cacgaacctg gagcgggtcg ccaacagccg gtcgcaggac
      841 accctgcggc tggccggccg tctcgcggcc gacctgcgct tcggccgcat cgacgagatc
      901 ctggccaccg gcctgcacgc ctatctgacg cagttcctcg agcggatcaa tacgctcggc
      961 gccggaatca gccgcgactt cctggtgccg atggccgact gaaacgccgg cgttcaccat
     1021 ggcgttgtgg ccgcgcattc gcggcagggc gcatccgcga tcacgcgcgg ggtcgtccga
     1081 atccttccat ggcatcacgc gagcgcgggt gtctgccccg cgacgagccc cccgtcgacg
     1141 ggagcgcttc gtggggtgcg ctcgggctgc atttcccgcc gtcgtcggga tagtccggcg
     1201 tcggtgtcgc cgcgacactg ccgacctcga tgtcgaggag cagacatgcc ggatccgaag
     1261 ccccaggagc tctcgctcgt gccgatcgaa agcgagcctc ccgttggcga cgcagcacct
     1321 tcgacgcggc gccgcggctc ggccgtcgtc tctctcgcgc cgagctcgcc ttcagcgccg
     1381 aggtcgtccg agcgtgcgca ccgcggcccc ggatggttcg attcgagctg ggacctgcag
     1441 acgggctgcg aagtccgcga aggctggcct cgcgaagccg gcctgttcga atggatcgag
     1501 tcgtggctcg ggcagccagg aatcgacgtg gcgcaatcgc tgtgcgatgc ctgaagcgtg
     1561 ggccggcgcc cgcggcggcg cggtgtcgtc cggtcgtgac ccgtggcgtc gtcagctgcg
     1621 agcgccctcg accgcgtcgc gcaccgcttc gcccaggtcg gtcacggcca tggcgtagta
     1681 gctcgaccag ttgtagcggg tgatggcgta gaagttggtc gtgccggcaa cgaaggtcgg
     1741 atcgtcggcg ccgttttgca gttcgaccag cgcgagcgcg ctgtcggtgg cagcggccga
     1801 ttcgggcagc gaggcaccga gtgccgacat ctgcccggca gtgaaggtcg gcaggatgtc
     1861 gggcgcgcgc agcgtcgcca ggttggcggc gtcgagcggc ggcaccaccg gatagcgaac
     1921 cgccaaacca tgccgccagc ccgactgcgc cagatagtgg gcgacgctgc cgatgacatc
     1981 ggccgcgctt cgacgcagat cgacgtggcc gtcgccgtcg aggtcgaccg cgaagcggtt
     2041 gaagctcgaa ggcatgaatt gcgccatgcc gatcgcgccg gcatagctgc cctgccagtc
     2101 gagcgggtcg atcgcctggt tgttgcacag aacgaaccag ctctcgagct cgccgcgaaa
     2161 gaactcgctg cgatcgctgc ggccggccgg gaagtcgaag gacagggtgg cgagcgagtc
     2221 gatgacgcga aagctcccca tgtgcctgcc gtagatcgat tcgacgccga cgatgccgac
     2281 gacaatctcg ggcggcacgc cgtagcgctc ttcggccagg cgcagccacg cctcgttgtc
     2341 gcgccagaac gaaacgccgg caccgatccg gatgggctcg acgaagcggg cgcggtaggc
     2401 ggcccagttc ttcgccgcgc cctgcggcgg cggcatgatg tagcgcgtca cggccggcat
     2461 gaaccgggcc tgggcgaggg cagcgcgaac ggccgctggc tcgaggccct gccgcagcgc
     2521 gacggcgtcg gcaaactgca tcacatcgtc gcggccgccg taggtgacga cgtcgggtgc
     2581 gtcgtcggtg gcgacagcgg cacgggggcg ccgcgccttt cgcttcgccg ccagcgccgg
     2641 cgctgccgtg gtggccagca aggcggcggc ggcgaggaag gtgcgtcggc ggcccgggtc
     2701 gatcatcgtt gcgattatcg agggatggtg gcacgcaggc ggcgtgcatc ggccacgaat
     2761 cggcgcgtca tgtcgcgaac agggcggcgt gcgccgtcgc gaccgtagcg ccgtgcctcg
     2821 agcgtctcga gggccgccgc aagcggctcg ccggcagcac cgagccggtc gcgcacctgc
     2881 gctgccagtg cccgcggtgg gtcgtgcggt gccgcggcga cgccgatggt ccgcaggctg
     2941 cgcttcatcc ggtcgagctg cctcacccag gggtcgaggc gatgccggtc gacccacgcc
     3001 cagccggcgg cggcgaccgc cagcgcgctg aggcagccga tcagcaacaa ggcgaggtcc
     3061 tcccatcccg gtgactggaa gccgaggttt ttcagcacga cgagctgctg gccgcgcgaa
     3121 tagttgagca cccactggtt ccagcggttg ttgaaggctt cccagccgac gcgcaggccg
     3181 accacgaacg ccgggctgac gctgtccagc gcctcggtca tcagtcccgg cggcggcacg
     3241 aggcggtggc ttcgaccgat ccggtccggg gccaccgcgg cggtgggatc ggcgcggacc
     3301 cagcccgctc ctgcctgcca gaactcggcc caggcgtgcg ccgagctctg ccggacgatc
     3361 cagaagccgt cgaccggcgt cggatcggtg ccttgatacc cggtgacgac gcgcgacgga
     3421 atgccggcgg ctcgcatgac gacgacgaag gcggcggcga agtgctcgca gaatccttcc
     3481 ttgcgatcga gccagaactc gtcgatgccg tcgcggccgt agtcgccggg tgagagcgtg
     3541 tagctgaagc ccgcgctgcc gatatagcga aggacggcgg cggcgaattc gctggcgctg
     3601 tcgccacggc gcagcggatc ggcgcgaaag cgtcgcgccc acgcgatcgt gcgcgggttg
     3661 tagccgggcg gcaggtcaag gctttcctcg agctcgcccg gtcgacgcgg tgctccgagg
     3721 gtgaagtgcg tatccgcgac ggcgtgaaag cggacccgat cgacgatcgg ccggtcggcg
     3781 agccattcga gatcgtcgcg ggcgacgacc cggtagccgt cgaccggcga gacggaaacc
     3841 gtcgcctcga gcaagggcag cgtggcgatg cgcaagggtt cgagagtgac ctcgtagccg
     3901 atcggcggtc cacgcaaggt gagcgcgggc cgacgggccg gcgcgccggc cggtgcgaac
     3961 ggcaggccga gcggacgcca gtcgacgccg tcgaaacggg tcagcacggg tccgcggaag
     4021 tagcgctgcg ccggcggcgg cggcgcaccg tcgaagcgca gtcgcatcgc caccgaatcg
     4081 tcgagcgcca cttcggcgat cgagcccatc ttcatgctgt tcgacagccc ggtggtcgac
     4141 accccgtcct gcggcacacc ccagagcggg ccgatccgcg ggaagaggac gaagaggagc
     4201 gccatcaccg gcgcgccgag cagcgccgtg cgcgccgcga ggccgctggc ctgacgcagg
     4261 ctcggctggc cgaccggcat gtgcgcgagg acgacggcgg taagcagccc ccacaccgag
     4321 acgagcatcg ccgcggcgac ggcaaccgat tgcgaataga gaaagtgggt gaggatgatg
     4381 aagaagccga ggaagaagac gacgaaggcg tcgcgccggg cccgcaactc gagcgtcttc
     4441 agtgccatca gcgagacggc cagggtgatg ccgggctcct tgccgagcaa cgtccggtat
     4501 gaccaccaag tgaggccgac cgcgatcgcg aggaccacca ccagcaccca gcggggtggc
     4561 aaggcgccgc cgacgagagc gagccggcca cgccagagaa ggacgaccgt cgtcagtgcg
     4621 atcgaccagg ccggcagatg cgaggcgtgc ggcaggaccg tccaggcgat cacggccagc
     4681 aggaagagcg tgtcgcgcgc ctcgcgcggc aatcgactcc agccgggcca aagcggcatc
     4741 cagccgggtg cacttgcggc ggccctcgtg ctcatccgcg cctcacgccc agagcgccaa
     4801 cgcttccagg caatgtcgtt gctgcacttc gccgctgccc ggcgaaagct cgacatcggg
     4861 aagacgcagg ccgaaatcgg cgcctgaacg cgttgcggcc agggtccacg cggcgaggcg
     4921 ggaaagccgg tcttcggccg cgagcgcacc gcaggcggcc cagtcgagcc agagctcgtg
     4981 gcggttcgag acgctcgtat cgcggctcac cagctcggcg ccggtttcga gcgcctgtgc
     5041 cgccttcttc caggcaacga gcttgagcgg gtcgccgcgt cgatacgcac gcacgccctc
     5101 gatctcgcca ccgtcgctgc tgcgcgacga gctcgggccg ccgggcaccg gccgcgcggc
     5161 gggcagtggt ggtgcgccgc tttcgggatg cggatagacg agcagccgtg cggccggacg
     5221 ccagatcgtc caggcacgaa agaggccgag cggaaaacgc gtctcggcgc tgagcgtcgg
     5281 cacctcgtgc aggccgcgtc gtggcggcac gaagctgacg cggggactcg cgcgtccgcc
     5341 ggcggcgacg tcgacccaga ccagtgtcga ttccggcgct tcgagcatcc gcaggccgat
     5401 gccgaagcgc gccgtgccgg ggctgctcag cacgacgtcg agcaccgccg gctcaccggc
     5461 gaacgccgcg tcgacaggcc gcaggtgcag cgtcagcccg cgcagggtgg cgtgcgtgac
     5521 gtgcatcgag acgacgccgc tgccggcgag aaggaaagtg aggacgtagc cgaggctcag
     5581 ctggtagttg atcgatgcca gcaacaggac gatcagcgtc aggccgaaca tccagccggc
     5641 ccgcgtcggc aggatgtaga cgttgcgctg cgtcagcgtc tgggtgtcgg tgcgcggaac
     5701 ccgcgcctgc caccagcggc gaaagcggcc acgcgtgaac gccgccggat tgagcgcagc
     5761 gatgtcgatc acggcagcgc gacggcctcg atcatcgccc gtacctgctc gacgcggcca
     5821 cggccggcgc cggccgccgg cagcaggcgg tgggcgatgg tctgcggcag cacgtactgc
     5881 acgtcgtcgg gcg
//
LOCUS       DASRWS010000653         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4276150, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000653 DASRWS010000000
VERSION     DASRWS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4276150"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2234)
                     /locus_tag="VFF72_12480"
     CDS             complement(<1..2234)
                     /locus_tag="VFF72_12480"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HZV94027.1"
                     /translation="MAEGLGLLRTLAQALRVWSWGGIACDLVVVNHEPASYLMALNRE
                     IVSLKEAHLSARQAEPGAAETSFHVLQSTDLQADELSTLRALARVRLNADGRPLSRYV
                     QELIDAHDGALEERQAVSTTAVAGVMGGEIVPRPPAGDFAQTSGEFRFDVTALVRPAR
                     PWINVLANADFGTQVSEAGGGYSWALNSRLNMLTPWSNDAVADPPGEWLLLQDLKTRE
                     AWSVAPSAAGAAEVEYRVAHGQGYSVITHRRGPLDVSVAWCVDAATSVKQARIRLVNR
                     GHRTLQLRLIGVAEWIVGAQRADRGTTLTSMATQAAANSEEATASPRGDEATSAARRM
                     TSLFCTQRDRLGGFGGGTAFFGLAGDSEDLLDWTCDRRESFDARGRFVVPDSYAQTSG
                     AGLDPCAAIAARLTLRAGESLDRVFLLGWSASPEGAQVLAKEAALVPPLSRLRDVRAV
                     WDDLLGATAVRTPDPLFDAMVNRWLLYQAIACRLWARAGFYQAGGAYGFRDQLQDAMA
                     LAWAAPQMLCRQIVLSASRQFAEGDVQHWWHAPTGAGVRTHFSDDLLWLPHAALHYLD
                     ATGDAAMLDESVPFLEGIAVPDGAEDAYYVPDIGPDPVSVYEHCARAIDHSLSVGAHG
                     LPLMGTGDWNDGMNRVGNEGRGESVWLAWFVIDVVGRFAPLAEQRGDSRRAERWRGAL
                     AGWQAATERQGWDGEWYRRAFFDDGSPLGSNSNEECRIDLIAQAWAVLSHAAPEGRAD
                     CAMRA"
BASE COUNT          340 a          817 c          800 g          321 t
ORIGIN      
        1 gcccgcatcg cgcaatcggc gcggccttcg ggcgcggcgt gcgaaagaac ggcccacgcc
       61 tgggcgatca gatcgattcg gcattcctcg ttgctgttcg agccgagcgg cgagccgtcg
      121 tcgaagaacg ctcgccggta ccactcgccg tcccatccct ggcgctcggt cgctgcctgc
      181 cagcccgcca gggcgccgcg ccagcgttcg gcgcggcgcg agtccccgcg ttgctcggcc
      241 agcggcgcga accggccgac gacatcgatc acgaaccagg cgagccagac cgactcgcca
      301 cgaccctcgt tgccgacccg gttcatgccg tcgttccagt cgcccgtgcc catcagcggc
      361 aggccgtgtg cgccgaccga caggctgtgg tcgatggcac gcgcgcagtg ttcgtagacc
      421 gacaccgggt cggggccgat gtcgggcacg taataggcgt cctcggcgcc gtccgggacg
      481 gcgatgccct cgagaaacgg cacgctctcg tcgagcatgg cagcgtcgcc ggtggcatcg
      541 aggtagtgca gcgcggcgtg cggcagccac agcaggtcgt cggagaagtg cgttcgcacg
      601 cccgcgccgg tcggcgcatg ccaccaatgc tggacatcgc cctcggcgaa ctggcgcgac
      661 gccgacagga cgatctgccg gcacagcatc tgcggcgccg cccaggccag ggccatcgcg
      721 tcctggagct ggtcccgaaa gccgtaagcg cccccggcct ggtaaaagcc cgcccgcgcc
      781 cacaggcggc aggcgatggc ctgatagagg agccagcgat tgaccatcgc gtcgaacagc
      841 ggatcgggcg tgcggacggc ggtcgcgccg agaaggtcgt cccagacggc gcgcacatcg
      901 cgaagacgac tgagcggcgg caccagcgcg gcttccttcg ccagcacctg cgcaccttcc
      961 gggcttgccg accagccgag caggaacacc cgatcgagcg actcgccggc gcgcaacgtc
     1021 agccgggctg cgatcgcggc gcacgggtcg agacccgcgc cactcgtctg cgcgtacgag
     1081 tcgggcacga cgaagcgtcc tcgcgcatcg aaggattcgc gtcggtcgca ggtccagtcg
     1141 agcaggtctt cgctgtcgcc ggcgaggccg aaaaaggcgg tgccgccgcc gaatccgccg
     1201 agccggtcgc gttgcgtgca gaaaaggctc gtcatccgtc gcgccgccga cgtcgcctcg
     1261 tcaccgcgcg gcgaggccgt cgcttcctcg ctgttcgctg ccgcctgcgt cgccattgac
     1321 gtcaacgtcg tgccgcggtc ggcgcgctgt gcgccgacga tccactcggc gacgccgatc
     1381 aggcgaagct gaagcgtgcg gtggccgcga ttgaccaagc gaatccgtgc ctgcttgacg
     1441 gacgtcgctg cgtcgacgca ccaggcgacg ctgacgtcga gcgggccacg ccggtgggtg
     1501 atgacgctgt acccctgtcc atgggcgacg cgatactcga cttcggcggc gccggcggca
     1561 ctcggcgcga cgctccacgc ctcgcgggtc ttcaggtcct gcagcagcag ccattccccc
     1621 ggcgggtctg cgaccgcatc gttcgaccat ggcgtcagca tgttgaggcg gctgttcaac
     1681 gcccagctgt agccgccgcc ggcctcgctc acctgggtgc cgaagtcggc gttggcgagg
     1741 acgttgatcc acggccgggc cggccggacg agtgccgtca cgtcgaagcg gaattcgccg
     1801 ctcgtctgcg cgaagtcgcc ggccggaggg cgcggcacga tctcgccgcc catcacgccc
     1861 gcgacggcgg tggtcgagac ggcttgccgt tcctcgaggg cgccgtcgtg ggcatcgatc
     1921 agctcctgga cgtagcgcga cagcggccgt ccgtcggcgt tgagcctgac gcgggccagc
     1981 gcccgcagcg tcgacagctc gtcggcctgg aggtcggtcg actgcaggac gtgaaagctg
     2041 gtctccgccg cgccgggctc ggcctggcgt gccgacaggt gcgcttcctt caggctcacg
     2101 atctcgcggt tgagcgccat caggtacgac gccggctcgt ggttgacgac gaccaggtcg
     2161 caggcgatgc cgccccagga ccagacgcgc aatgcctgcg ccagcgtgcg cagcaggccg
     2221 agaccttcgg ccacgccggc agagacgagg acgatgggcc gatcgcccga tatgccga
//
LOCUS       DASRWS010000654         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2860730, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000654 DASRWS010000000
VERSION     DASRWS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2860730"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFF72_12485"
     CDS             <1..266
                     /locus_tag="VFF72_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HZV94028.1"
                     /translation="FIGADAETGWLPEAIERDERGYVRTGDAVAKSGRWKIERDPYLL
                     ETSVPGIFACGDVRASPIKRVATAVGEGSMAIAFVHQFLAHVA"
     gene            complement(347..811)
                     /locus_tag="VFF72_12490"
     CDS             complement(347..811)
                     /locus_tag="VFF72_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94029.1"
                     /translation="MHRQIPAAVLVCGLFAAGASFAQAPSGAPAGSTGMCKDGTYTTH
                     STKKGACAGHKGVKDWFEAAAAAPAMKPAETTTSTKAMPAPSATTTSTTKTASMAPAA
                     GGGPGQVWVNTSTKVYHCQGTKYYGKTKHGEYMTEAAAKAAGDHADHGKACS"
     gene            1075..2922
                     /locus_tag="VFF72_12495"
     CDS             1075..2922
                     /locus_tag="VFF72_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV94030.1"
                     /translation="MDDHRKASHAQGKARPDKRPTTRHLRVAAALASFGVALPMLARA
                     GCQIQTLDLPVKMVGSRAVATVGIGGTPVPLTVDSGAFFSFLTDAAAAQFKLRLRWTD
                     GKVEGLTGGVDVHVTTVENLQLLGGSIPKIGFIVGGNEPGAGTMGLIGRNVLSATDTE
                     YDLAHGVIRFVFPNDDCAKANMAYWAGASPVTQVDLMPDYRSRTPAIRAHVMLNGKEL
                     VALFDTGATTIVSAGAARRAGVAEADMTPAGTSYGAGRGSAKLWTARFDKFELGGEAI
                     LHNDLEVADFDMDDADMLLGIDFFLSHRIYVSKRQDRMFFTYNGGRVFARNKSESARP
                     ATFVADTAAAGGAATTADELERRGQASAARHEYESALADLNRACELAPASSAFFAERG
                     VIQERLKRPAKALEDFDKALELDPTRVDARFQRARLRLSEKNRDGAKVDLDVLDQTLA
                     PQAQMRLPMSQMYLSLEQPARALAQVNQWLPAHPHEVKREDALNTRCWTRLMLGMELD
                     KALDDCDEAIDGDSKNPDYLDSRGWVYLRLGKYEKAQRDFDRSLELRPAGAWSLYGRA
                     LTKTRLGNSAQSEADLAAARKLRPNIDLEVAQIHITNEPAPPPTPTPKP"
     gene            3218..>3753
                     /locus_tag="VFF72_12500"
     CDS             3218..>3753
                     /locus_tag="VFF72_12500"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94031.1"
                     /translation="MRFSTQGVPARHRVAFWRDAVCAAFVRLDLDCDPARPFSSELVV
                     RRTARLDLIDVGGSPQEVRRSARMIDADHAESLIVMLQREGRGAARQGELDMTLAPHG
                     LTVLDSRRPYALRFSTPFRQTVVKVPLAMLAERIGPAEYCVGGALGSGSALVRLAGAA
                     IDELAREPRDGVAQHLAPV"
BASE COUNT          615 a         1282 c         1278 g          578 t
ORIGIN      
        1 tcttcatcgg cgccgacgcc gagaccggct ggctgcccga ggccatagag cgcgacgaac
       61 gcggctacgt gcgcaccggc gatgcagtgg cgaaatcggg gcgctggaag atcgagcgcg
      121 atccctatct gctggagacc agcgtgccgg gcatcttcgc ctgtggcgac gtgcgggcca
      181 gcccgatcaa gcgggtggca acggcggtgg gcgaaggcag catggccatc gccttcgtgc
      241 accagttcct ggcacacgtc gcctaggcgg tcgcggcagc accgcgcgca acacggctcg
      301 ggttcgagcc gccccgcgac gcggcgccgg ctctcgcgcg cctgcctcag gagcaggcct
      361 tgccgtgatc ggcgtgatcg cccgcagcct tggcggcggc ttccgtcatg tactcgccgt
      421 gcttggtctt gccgtagtac ttcgtgccct ggcagtgata gaccttcgtg ctggtgttga
      481 cccagacctg gccggggcca ccgccagcgg ccggggccat cgacgccgtc ttcgtcgtcg
      541 aagtcgtcgt cgccgacggc gcgggcatcg ccttggtcga agtcgtcgtc tcggcgggct
      601 tcatcgccgg cgcggccgcg gcggcctcga accagtcctt cacgcccttg tggccggcgc
      661 aggcgccctt cttggtcgag tgcgtcgtgt aggtgccgtc cttgcacatg cccgtcgacc
      721 ccgcaggggc gccggaaggg gcctgggcga acgaggcacc ggcggcgaac aggccgcaga
      781 cgaggaccgc ggcaggaatt tgacgatgca tcgaaggctc caggaattga ggggtgaagg
      841 gcgacattgt cgtccgcagt gcgtgcaggg tctggtgcgg gaacgccgtg cgccttgcgg
      901 cccggcgatt ttcgtgcccg ggccgtgtcg tcaccgatgt acgtgccgat cccctttggc
      961 ggaacgctcc tctgctcgcc gggccggtcg ccttcgctac cgtaaagttg cagtgctgac
     1021 gagagggcgc ccgccgacgc gtcgcgcgac cgggccgacg aggagaacga ccccatggac
     1081 gatcacagga aggcgtcgca cgcgcaggga aaagcacgac cggacaaacg cccgacgaca
     1141 cggcacctgc gagttgcggc cgcgctcgcg tcgttcggcg tggcgctgcc gatgctggcc
     1201 cgcgccggat gccagatcca gacgcttgat ctgccggtga agatggtcgg ctcgcgcgcc
     1261 gtcgccacgg taggcatcgg cggaacacct gtgcctctga cggtggacag cggcgccttc
     1321 ttcagcttcc tgacggacgc agcggccgcg cagttcaagc tgcgcctgcg atggaccgac
     1381 ggaaaggtgg agggcctgac cggcggcgtc gacgtgcacg tgacgacagt cgagaacctg
     1441 cagttgctcg gcggaagcat tccgaagatc ggattcatcg tcggcggcaa cgagcccggt
     1501 gccggcacca tggggctgat cggacgcaac gtgctctccg ccaccgatac ggaatacgac
     1561 ctggcgcacg gcgtgatccg attcgtcttt ccgaacgacg actgcgccaa ggccaacatg
     1621 gcgtattggg ctggcgcctc gcccgtcacc caggtcgacc tgatgcctga ttaccgctcc
     1681 aggacgccgg cgatccgggc ccacgtcatg ctgaacggaa aggagctcgt agcgctgttc
     1741 gacaccggcg ccaccacgat cgtttctgcc ggagcggccc gtcgcgccgg cgttgccgaa
     1801 gccgacatga cgcctgccgg cacgagctat ggcgcgggtc gcggcagcgc gaagctgtgg
     1861 acggcccgct tcgataaatt cgagctcggc ggcgaggcga tcctgcacaa cgacctcgag
     1921 gtggccgact tcgacatgga tgacgccgac atgctgttgg gcatcgattt cttcctgtcg
     1981 catcggatct acgtctcgaa gcggcaggac aggatgttct tcacctacaa cggcggcagg
     2041 gtgttcgctc gcaacaagag cgagtccgcc aggccggcga ctttcgtcgc cgacacggcc
     2101 gccgccggcg gcgcggcgac caccgccgac gagctcgagc gccgcggcca ggcctcggcc
     2161 gcacgccacg agtacgagag cgcgctggcc gatctgaacc gggcctgcga gctcgctccg
     2221 gcgtcgtcgg ctttcttcgc cgagcggggc gtcatccagg aacgactgaa gcgcccggcg
     2281 aaggcgctgg aggatttcga caaggccctg gagctcgacc cgacgcgggt tgacgcccgc
     2341 tttcaacgag cgcggttgcg cttgagcgaa aagaatcgcg acggcgccaa ggtcgacctc
     2401 gacgtgctgg accagacgct tgcgccccag gcgcagatgc gccttcccat gtcgcagatg
     2461 tacctgagtc tcgagcagcc ggcgcgggcg ctcgcgcagg tgaaccaatg gcttcctgcc
     2521 catccgcacg aggtcaagcg cgaggatgcg ttgaacacgc ggtgctggac gcggctcatg
     2581 ctgggcatgg aactcgacaa ggccctcgac gactgcgacg aagccatcga cggcgattcg
     2641 aagaacccgg actacctgga cagccgcggc tgggtctacc tgcggctggg caaatacgaa
     2701 aaggcgcagc gcgacttcga ccgcagcctc gagcttcggc ccgccggcgc ctggtcgctc
     2761 tacggccgcg ccctgacaaa gacgcgcctg ggcaactccg ctcagtccga ggccgacctc
     2821 gcggcagcgc gcaagttgcg gccgaatatc gatctcgagg tggcgcaaat ccacatcacg
     2881 aacgaaccgg cgccgccgcc gacgccgacg ccgaaaccat gagcggtgtg cccaggcacg
     2941 aacactcctg gccgccggcg ggctgccaac acggccaagg ccgtcgcctg atacgggcac
     3001 cctccgcaaa cccgttgtcg cggatcgtgt gacctccatg gtgcggaggc cacgaccacg
     3061 gattggatag ccaggcgtac gagtcgacac gcctcgacag cgaggaagtc aacaagtcgg
     3121 cgttgcgttc ctgcgcggat tgggcacact cgatcgcccg cgcagccacg gcccatcccg
     3181 acaagacgtg cccgcatcga acgaatccgc aggcgccatg cgcttcagca cccagggcgt
     3241 gccggcgcgg caccgcgtcg cgttctggcg agacgccgtc tgcgctgctt tcgtccggct
     3301 cgatctcgac tgtgaccccg cacgaccgtt ttccagcgag ctggtggttc gtcgcacggc
     3361 ccgtctggat ttgatcgacg tcggcggcag cccgcaggag gtgcggcgca gcgcgcgcat
     3421 gatcgacgcc gatcacgccg agagcctgat cgtcatgttg cagcgcgaag gcaggggcgc
     3481 cgcccgacaa ggtgagctcg acatgactct cgcaccgcat ggcctcacgg ttctcgacag
     3541 ccgtcggccc tatgcactga ggttttccac accgtttcgt cagaccgtcg tcaaggtacc
     3601 gctggcgatg cttgccgagc gcatcgggcc tgcggagtac tgcgtcggcg gcgcgctggg
     3661 cagcggctcg gcgctcgtcc gcctcgccgg cgccgcgatc gacgaactgg cgcgcgaacc
     3721 tcgcgacggc gttgcacaac acctcgcccc ggt
//
LOCUS       DASRWS010000655         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1722483, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000655 DASRWS010000000
VERSION     DASRWS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722483"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VFF72_12505"
     CDS             complement(<1..1820)
                     /locus_tag="VFF72_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZV94032.1"
                     /translation="MSSAPPFQRFVADELALAPALVTRILAGTLQLLGQSGDGGLSAS
                     ERAHHSEIVKALQRDAKLYEKTFVESLNRRVADELDGPTEMMVAEVKPGIVGLELMDE
                     SRVEIDIEISRAMQLIDSTAEWELRELQTFTSTLTGQRHVNADSNPFRPLVYASALWD
                     AACAVVSSPTQRTIILRTSAGVIAGLLKNAWAAASTRLEAQGVQPGAYRTVVLPSGAS
                     FGRFPAPELARTGALSALLSSMPSSSADPTTTNPGSHGAPAGMAGVRSDGTNRRSPEL
                     EQALLRLDELLRQLPAESSLSGRGEGQTGRIEQHRAALVASATEPVDRQVIELVTRLF
                     DSLLADSQIPPAFRPTLARVQIAVLRVALSDRSVLDAYEHPVWRLIDRIGEVSLGYSR
                     PEDPRLAAFLAFSSAVAEEMAGSSLPDTSLFRRGLNRLDAFLAEQLQGQLRTAQPSSE
                     ALQLAERREILQQHLSQRLTDQMVAVRTTPAIRRFVTGTWASVIADDMLRQGEQSDAT
                     LSDLKTVNDLMWSLKIPDHPQSRQRLLQLLPGLLQRLRNGMEAVGLPVPEQQSVLNEL
                     MVIHTEALRPGTRGTGALTPEEIVQRMRDEVVPEAGPARS"
     gene            complement(1962..>2892)
                     /gene="groEL"
                     /locus_tag="VFF72_12510"
     CDS             complement(1962..>2892)
                     /gene="groEL"
                     /locus_tag="VFF72_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZV94033.1"
                     /translation="KSGRPLLIVAEEIEGEALATLVVNTIRGILKVVGVKAPGFGDRR
                     KAMLEDIAILTGGKVISEEVGLTLEKVQLADLGQAKRVEVAKENTTIIDGAGAAADIE
                     ARVKQVRVQIEEATSDYDREKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALH
                     ATRAAVEEGIVAGGGVALLRARQAAGTIKGDNPDQDAGIKLILRSIEEPLRIIVQNSG
                     DEPSVVVNAVLGGKGNYGYNAANGQYGDMIEMGILDPTKVTRTALQNAASVASLMLTT
                     EAMISEAPKEDAPAGGGMGGGMGGMGGMGGMDM"
BASE COUNT          449 a          962 c          958 g          523 t
ORIGIN      
        1 aagctgcgcg cggggcccgc ctcgggcacg acttcgtcac gcatccgctg cacgatctcc
       61 tccggcgtga gcgccccggt gccccgcgtt cccgggcgca gagcctcggt atgaatgacc
      121 atcaactcgt tgaggaccga ctgctgctcg ggcaccggca ggccgaccgc ttccatgccg
      181 ttgcgcaatc gctgcagcag tcctggcaga agctgcaaca gccgttgccg gctctgcggg
      241 tgatcgggaa tcttcaggct ccacatcagg tcgttgacag tcttcaggtc gctcaatgtt
      301 gcgtccgact gttcgccctg gcgcaacatg tcgtcggcaa tcacgctggc ccaggttccg
      361 gtgacgaagc gccggatcgc cggcgtcgtt cgcaccgcga ccatctggtc ggtgagcctt
      421 tgtgagaggt gctgctgcaa tatctcgcgc cgttcggcga gctgcagcgc ctcggacgag
      481 ggctgcgctg tcctgagttg accctgcaac tgttcggcaa ggaacgcatc gagccggttc
      541 agcccgcgcc tgaagagcga ggtgtcgggc agcgacgatc cggccatctc ctccgccacc
      601 gcgctgctga acgcgagaaa cgccgcgaga cgcgggtcct cgggccgcga gtagccgagg
      661 ctgacctcgc cgatgcggtc gatgagacgc cagacgggat gttcgtaggc gtcgagaacg
      721 gagcggtcgg aaagggcaac gcgcaggacc gcgatctgca cccttgcgag cgtgggccgg
      781 aacgccggcg gaatctgcga atccgccaga agcgaatcga agaggcgcgt caccagttcg
      841 atgacctgcc ggtcgaccgg ctccgtggcg ctggccacga gcgctgcgcg atgttgctcg
      901 atgcggccgg tttgtccttc gccgcgtcct gacagcgagg attcggccgg caactggcga
      961 aggagttcgt cgagtcgcag cagcgcctgc tcgagttcag gactgcgccg gttcgtgccg
     1021 tcgctacgca cgccggccat gcccgccggc gcgccatgcg agcccgggtt cgtcgtcgtc
     1081 ggatcggcgc tcgaagacgg catgctcgag agaagggcgc tcaaggcacc tgtgcgcgcc
     1141 agctcgggcg ccggaaagcg gccgaagctc gctcccgagg gcagaaccac tgttcgatag
     1201 gcgccgggct ggacgccctg ggcttcaagt cgtgtcgacg cggcggccca cgcgttcttc
     1261 agcaacccgg cgatgacgcc ggccgacgtg cgcaggatga tggtgcgctg ggtcggcgag
     1321 ctgacgacgg cgcacgctgc gtcccacagg gccgacgcgt agacgagcgg tcgaaaggga
     1381 ttcgagtcgg cgttgacgtg acgttggccg gtcaacgtcg aggtaaacgt ctgaagctcg
     1441 cgcagttccc attccgccgt cgagtcgatc agctgcatgg cacgcgagat ctcgatgtcg
     1501 atctcgacgc gcgattcgtc catcagctcg agtccgacga ttcctggctt gacctcggcg
     1561 accatcattt cggtcggtcc atccagctcg tcggcaacgc gccggttcag cgattcgacg
     1621 aatgtctttt cgtagagctt cgcatcgcgc tgcagcgcct tgacgatctc ggagtggtgc
     1681 gcgcgttcgc tcgcgctcaa cccgccgtcg cccgattggc cgaggagctg cagcgtgccg
     1741 gcgagaatac gcgtgacgag cgccggcgcg agggcgagct catcggcgac gaagcgttga
     1801 aacggcggcg cggacgacat ggacttcgag catcaaggga agccccatta tctcgcgctg
     1861 gatgcgatgc atcgctcggc cggcggtgcg tcacgccggt tcgcggggac aaaaaagggc
     1921 tgccgaagca gcccttttga atccaggcgc gtgcgccgga cttacatgtc catgccgccc
     1981 atcccgccca tgccgcccat gccgccgccc atgccgccgc cagccggagc gtcttccttc
     2041 ggcgcttcgg agatcatggc ttcggtggtg agcatcagcg atgcgacgga ggctgcgttt
     2101 tgcagcgccg tgcgcgtgac cttcgtcggg tcgagaatgc ccatctcgat catgtcgccg
     2161 tactggccat tggccgcgtt gtagccgtag ttgcccttgc cgccgagcac ggcattgacg
     2221 accacgctcg gctcgtcgcc cgagttctgg acgatgatgc gaagcggttc ctcgatcgaa
     2281 cgcaggatca gcttgatgcc agcgtcctga tccgggttgt cgcccttgat cgttccggcg
     2341 gcctgacggg cgcgaagaag cgccacgcca ccgccagcga cgatgccttc ttcgacggcc
     2401 gcacgggtcg cgtgcagcgc gtcttcgacg cgcgccttct tctccttcat ctcgacctcg
     2461 gtggcagcgc caaccttgat cagggcgacg ccgccagcca gcttggccac gcgttcctga
     2521 agcttttcgc ggtcgtagtc ggaagtcgct tcctcgatct gcacgcgcac ctgcttgacg
     2581 cgggcctcga tgtcggccgc ggcgccggcg ccgtcgatga tcgtggtgtt ctccttggcc
     2641 acttcgacgc gcttggcctg gcccagatcg gcgagttgca ccttttcgag cgtcaggccg
     2701 acttcttcgg agatcacctt gccgcccgtc aggatggcga tgtcttcgag catcgccttg
     2761 cgccggtcac cgaagcccgg cgccttgacg ccgacgacct tgaggatgcc gcggatcgtg
     2821 ttgacgacca gcgtcgccag tgcctcgcct tcgatttcct cggcgacgat cagcagcgga
     2881 cgacccgact tg
//
LOCUS       DASRWS010000656         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2284859, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000656 DASRWS010000000
VERSION     DASRWS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284859"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /gene="fliF"
                     /locus_tag="VFF72_12515"
     CDS             complement(<1..934)
                     /gene="fliF"
                     /locus_tag="VFF72_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZV94034.1"
                     /translation="MDNAVALKPASQAALTDTFAGRIRAMPGRSKAAFLVGLAALAGV
                     VVAMTMWASQGDYKVLYANLSDKDGGAVIAQLSQMNVPYRMSEGGSAILVPAGQVHDL
                     RLKLATAGLPKGTISGFELMDSARFGQTQFQERLTFQRGLEGELTRSITSLSAVESAR
                     VHLALPNQNGFFREQQKPSASVLLSLRPGRTLDRAQLAGIVHLVSSSVPEMSPKAVSI
                     LDQSGTLLTGGADGAQTGLDAQQLQYVAQVESGYTRRIRELLEPIVGADNLRATVAAD
                     VDFSQTEATSEEFKPNQGAQASVSIRSQQTNEQGA"
     gene            1145..1429
                     /gene="fliE"
                     /locus_tag="VFF72_12520"
     CDS             1145..1429
                     /gene="fliE"
                     /locus_tag="VFF72_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZV94035.1"
                     /translation="MARAGLGTDGVPLAQRSPVAGAGFQQALGQALDAVSRSQNDATE
                     MQRQVQLDNPTVSLEQTMVAMQKAQIGFSATLQVRNKLVQAYTDIMGMQV"
     gene            complement(1559..2290)
                     /locus_tag="VFF72_12525"
     CDS             complement(1559..2290)
                     /locus_tag="VFF72_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV94036.1"
                     /translation="MSADVAPHVLVVDDEADVRDLVVEYLSKNDMRVSAATSGAAMFE
                     LFDREPIDLVLLDLKLPGEDGMQLARTLRERAAVPIVFLTGRNEEADRVMGLELGADD
                     YVTKPFSPRELLARVRAVLRRYQVQATLPERDHSRRAFRFSGFELNLRTRRLRSPVGE
                     SVDLSNGEFSLLNALCRAPRRVLSRDQLLSMSRLHEAEVYDRTIDVQVRRLRLKIEAD
                     AANPSLIVTERGAGYRLAAEVETLF"
     gene            2399..>3856
                     /locus_tag="VFF72_12530"
     CDS             2399..>3856
                     /locus_tag="VFF72_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZV94037.1"
                     /translation="MAIQDGVGATATKAATWRRSPWRLTLSVWLLLVAFSVAFLWHLR
                     REALFSQGREMTLLSAALTDDITRGLRGAQEGLHAVRAELRDHRLPLAGPEARRALHT
                     RADLMPLVDRLWLVGAGGQVLADSDATPAPDAATFAPPLGGLGADAMAVSRPFVDPPS
                     GQRFVALAVRYDGPDGTPGWIVAGLPASALLGAFSAAAPGADVRMDVFRSDGVLLASA
                     NEPSGSARAGAMVDGRTAAAPVVGMRGPVDGTRSLADQRVVPLYGLQVVVSRDLGAIL
                     AGWHAAVAASAAALLLLLVVMLFALGRVQLAERRRGEAQRALQAQLGRAGRLEALGTL
                     AGGVAHDFNNVLAGIVGYAELAQDAAPAGGEQARHLDRVLQAALRGKSMVERILMFSR
                     GGAKSSVSFEIEAVVEEVLALLSSTLRPGIVLERDLEAAAARVRGDPAQAFEAIMNLC
                     TNALQSMPEGGRLAVRTERIRIASDRVLSHSRLAAG"
BASE COUNT          531 a         1349 c         1384 g          592 t
ORIGIN      
        1 cggcgccctg ctcgttggtc tgctggcttc ggatcgagac gctcgcctgc gcgccctggt
       61 tgggcttgaa ttcctccgac gtcgcctcgg tctgcgagaa atcgacgtcg gcagcgacgg
      121 tcgcgcgcag gttgtcggcg ccgacgatcg gctcgagcag ctcgcggatg cgacgggtat
      181 agccgctctc gacctgcgcc acgtactgca gctgctgcgc gtcgaggccg gtctgcgcgc
      241 cgtcggcgcc gcccgtcagg agggtgccgc tctggtcgag gatgctgacc gccttcgggc
      301 tcatctcggg cacgctcgac gaaacgaggt ggacgatgcc ggcgagctgg gcgcggtcga
      361 gggtgcgtcc tgggcgcagc gacagcagca ccgaggcgct gggcttttgc tgctcgcgaa
      421 agaatccgtt ctggttgggc agcgccagat ggacgcgcgc gctttcgacg gccgacagcg
      481 aggtgatcga acgcgtcagc tcgccttcga ggccgcgctg gaacgtgagc cgctcctgga
      541 attgcgtctg cccgaagcgg gccgagtcca tcagctcgaa acccgagatc gtgcccttgg
      601 gcaggcccgc cgtggcgagc ttgagccgca ggtcgtgcac ctggccggcg ggcaccagga
      661 tcgccgagcc gccctcggac atgcgatagg gcacgttcat ctgcgacagc tgggcgatga
      721 cggcgccgcc gtccttgtcg gagagattgg cgtagaggac cttgtagtcg ccctgcgagg
      781 cccacatcgt catcgcgacg acgacgccgg cgagcgccgc caggccgacc aggaacgccg
      841 ccttgctgcg cccgggcatc gcccggatcc ggccggcgaa ggtgtcggtc agggcagctt
      901 gcgatgcggg cttgagggcg acggcgttgt ccatgggatt cgtgttcgct ggccgcgcgg
      961 agtcggcgtg cagggacctc gggttgacgg acgattattg ggatcggccc tgtcgcggat
     1021 gaccggaaca gccgggattt gggcggccag ttcgcgccac cgccggccgc ccggccgcct
     1081 acgatggcgg ccaaggaaaa aggccgtgaa cgtcaccctc aagcccttcg atttcgcgca
     1141 ggccgtggcc cgtgccggac tcggcaccga cggggtgccg ctggcacagc gcagcccggt
     1201 cgccggcgcc ggattccagc aggcgctcgg ccaggcgctc gacgcggtga gccgctcgca
     1261 gaacgacgcg accgagatgc agcgccaggt gcagctcgac aacccgaccg tcagcctcga
     1321 gcagacgatg gtggcgatgc agaaggcgca gatcggcttt tcggcaaccc tgcaggtgag
     1381 aaacaagctc gtccaggcct acaccgacat catggggatg caggtgtagc gtccgccgct
     1441 ttcctctgtc tccgtctccg tctccggcac gcgcgccacg tgtgccgcgt gtgcctcgtc
     1501 gaacgccgcc ggcgtcgacg ccgcggcgac ggtcgtcgcg ccgcgcacct cgccccgatc
     1561 agaacagcgt ctcgacctcg gcggcgagcc gatagccggc gccgcgctcg gtcacgatca
     1621 ggctcggatt ggcggcgtcg gcctcgatct tcagccgcag gcgtcgcacc tgcacgtcga
     1681 tggtccggtc gtagacctcg gcttcgtgca ggcgtgacat cgacagcagc tggtcgcggc
     1741 tcagcacgcg cctcggcgcc cggcacaagg cgttgaggag gctgaactcg ccgttcgaga
     1801 ggtccaccga ttcgccgacc ggcgaacgca gccggcgggt ccgcaggttg agctcgaaac
     1861 cggagaaccg gaacgcgcgc cgcgagtggt cgcgctcggg cagcgttgcc tgcacctggt
     1921 agcgacgcag cacggcacgc acgcgcgcca gcagctcgcg cgggctgaac ggcttggtga
     1981 cgtagtcgtc ggcgccgagc tcgaggccca tcacgcgatc ggcttcctcg ttgcgtccgg
     2041 tgagaaagac gatggggacg gccgcgcgct cgcgaagcgt ccgggccagc tgcatgccgt
     2101 cttcgccggg cagcttcaga tcgagcagga cgaggtcgat cggctcgcgg tcgaacagct
     2161 cgaacatcgc cgcgcccgac gtcgcggcgc tgacgcgcat gtcattcttc gacaggtatt
     2221 cgaccaccag gtcgcgaacg tcggcttcgt cgtcgacgac caggacgtgc ggggccacgt
     2281 cggcactcat gagccattgt gtagcgtggc gcgcttccgg tctgtcaagc gaatgcaaac
     2341 ttccttgaag tggggacaac gaacgatcgc cggcgcccca aacgcgccag actcggccat
     2401 ggcgatccag gacggcgtcg gcgcaacggc cacgaaggca gccacctggc gacgatcgcc
     2461 gtggcggctg acgctgagcg tctggctgct gctcgtcgcc ttcagcgttg ccttcctgtg
     2521 gcatctgcgc cgtgaggcgc tcttctccca ggggcgcgag atgacgctcc tctccgcggc
     2581 cctgaccgac gacatcaccc gcggccttcg cggcgcgcag gaaggcctgc atgccgtgcg
     2641 cgccgagctt cgcgaccacc gcctgccgct ggccggcccc gaggcgcggc gcgcactgca
     2701 cacgcgcgcc gacctgatgc cgctggtcga caggctctgg ctcgtcggag cgggcggaca
     2761 ggtgctcgcg gattcggacg cgacgccggc gcccgacgcg gccactttcg cgccgccgct
     2821 gggtgggctc ggcgcggacg ccatggcggt gagccgcccc ttcgtcgacc cgcccagcgg
     2881 tcaacgcttc gtcgccctcg cggtgcgcta cgacggcccc gacgggacgc ccggctggat
     2941 cgtcgccggg ctgcccgcct ccgcgctgct cggcgcgttc tcggcggcgg cgccgggggc
     3001 cgacgtccgg atggacgtct ttcgcagcga cggcgtcttg ctggcgagcg ccaacgaacc
     3061 gtcgggatca gcccgcgccg gggcgatggt cgacggacgg acggccgccg cgccggtcgt
     3121 cggcatgcgg ggcccggtcg acggcacgcg aagcctcgcc gatcagcgcg tcgtgccgct
     3181 ctacggactc caggtcgtcg tctcgcgcga cctcggcgcg attctggcgg gctggcatgc
     3241 cgccgtcgct gcgtcggcgg cggcgttgct gctcctgctg gtcgtcatgc ttttcgccct
     3301 gggtcgcgtt caactcgccg agcgccgtcg tggcgaggcg cagcgggcgc tccaggcgca
     3361 actcggtcgt gccggccggc tcgaagcgct cggcaccctc gccggtggcg tcgcccacga
     3421 cttcaacaac gtgctggccg gcatcgtcgg ctacgccgag ctggcgcagg acgcggcacc
     3481 cgccggcggc gagcaggccc gtcacctcga tcgtgtcctg caggcggcgc tgcgcggcaa
     3541 gtcgatggtc gaacgcatcc tcatgttcag tcgcggcggc gcgaagtcgt cggtgtcgtt
     3601 cgagatcgaa gcggtcgtcg aggaagtcct cgcgctgctg tcgtcgacct tgcgcccggg
     3661 catcgtcctc gagcgcgacc tcgaggcggc ggcggcgcgc gtccgtggcg atccggcgca
     3721 ggccttcgag gcgatcatga acctgtgcac caatgccttg cagtcgatgc ccgaaggcgg
     3781 gcggctggcc gtccgcaccg aacggatccg gatcgcctcg gaccgggtgc tgtcgcacag
     3841 ccgcctggcc gccggc
//
LOCUS       DASRWS010000657         7730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1131286, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000657 DASRWS010000000
VERSION     DASRWS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131286"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..420
                     /locus_tag="VFF72_12535"
     CDS             70..420
                     /locus_tag="VFF72_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94038.1"
                     /translation="MIRRLPAESPVRKPDGDADLLRLVGSAANETVDAISVLPSTRVG
                     AALAHIAQRYSRVLRTTPRMLAEAIQEARDVVRKGAAMPAGRQIAKATTDPVDALPEP
                     DAVPPRDGANAPHP"
     gene            complement(881..977)
                     /gene="ffs"
                     /locus_tag="VFF72_12540"
     ncRNA           complement(881..977)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFF72_12540"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1020..2153)
                     /locus_tag="VFF72_12545"
     CDS             complement(1020..2153)
                     /locus_tag="VFF72_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HZV94039.1"
                     /translation="MATDPADPSPLSYGAAGVDYDVIDPLKLAAQRAAASTGRHLDAR
                     GFSEVEASRGESAYVVDVGPFYLASIVECLGSKVLVADAVEQLTGKSAYAAIAQDTIA
                     MAVNDIVTVGATPLVVQAYWAAGASQWFADGDRSQALVAGWKAACDHCGIAWGGGETP
                     ALAGVVESGRIDLAASCTGIVSPKNRLCVGEHLAVGDAIVLLASSGIHANGVSLARQL
                     AARLPRGYMTPLSGSALPIDFGDALLEPTVLYPPITEAIFRAGVRPHYAANITGHGWR
                     KLMRHRADLVYRIHTLPDVPPVLRFIQEEARMSDHDAYGTFNMGAGFAVFVAAEDAER
                     TAAAARKAGIDAWVAGTVEAGPKQVVIEPLGVTFEGHELGLRI"
     gene            2247..4034
                     /gene="dnaX"
                     /locus_tag="VFF72_12550"
     CDS             2247..4034
                     /gene="dnaX"
                     /locus_tag="VFF72_12550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZV94040.1"
                     /translation="MPSTVLARKYRPRNFEAMVGQTHVVQALANALTQQRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCTGVDGLGGVTAHPCGVCSACRDIDGGRFIDYVELDAAS
                     NRGVDEISQLLDQSVYKPVVGRFKVYMIDEVHMLSAHAFNAMLKTLEEPPDYLKFVLA
                     TTDPQKVPATVLSRCLQFNLRPLAPQTIATHLESVLAQESVGFDAVGLRLIARAARGS
                     MRDALSLADQAIAYGGGAVEVDPVRRMLGSVDRDHAVRILDALARADGRALVGAVDHL
                     RELGLSAAGTLEELATLLQEMAIAQAVPSSDPIDDPDAPAIVRLAALLEADHTQLLYS
                     ITLTGRAELPLAPDEYSGLVMVLLRMLAFAPRGAADSAPKAGPAPIAESTSPRNRAPL
                     ATSASVESSMANASALHKETVAGKPESLREASTAPANDAEATAATGSVANVPSPAEPG
                     APSASPAPAPSDRDAADRWFEIVQRMIGAATIGAMVRELALQSECFAIVDTADEQLWR
                     LRVERDNLRSAGLLERLQTALGEGAQRRVRIEVEPGRALDSPAERLAAERRRRQLEAE
                     RTIHDDPLVKALLAQYHTARVVPGSVKPL"
     gene            4054..4380
                     /locus_tag="VFF72_12555"
     CDS             4054..4380
                     /locus_tag="VFF72_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZV94041.1"
                     /translation="MMKGQLAGLMKQAQAMQDNLKKAQDELATIEVEGQSGAGLVKVT
                     MTCKHDVKRVTIDPSLVGDDKDMLEDLVAAAFNDGVRRAEAVSQEKMGKLTAGMPLPP
                     GMKLPF"
     gene            4492..5082
                     /gene="recR"
                     /locus_tag="VFF72_12560"
     CDS             4492..5082
                     /gene="recR"
                     /locus_tag="VFF72_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZV94042.1"
                     /translation="MAAEASLADLVEALRRLPGVGVKSAQRMAYHLLQHDRDGARRIA
                     ESLQRAVARVQHCQLCHTFTEDVVCSICRDPSRDRRQLAVVETPADQAALERSGSFRG
                     HYFVLMGRLSPLDGIGPSEIGARELLDRAGDGVVEEVIVATNFTAEGEATAHVLAEAL
                     QSRGVRVTRLARGVPIGSELEYVDLGTIAHAFVDRR"
     gene            complement(5122..6825)
                     /locus_tag="VFF72_12565"
     CDS             complement(5122..6825)
                     /locus_tag="VFF72_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZV94043.1"
                     /translation="MTTLPRLPAGPQRFLALATLLLLLAGCVSAPLSTHPNASARADA
                     GTDVAVAAATPSPLPRPPDAGASATPASETAASQGDESVAAPVPIDPERPDVHINIDD
                     RSARVDLWQRVRKGFAIPDIDNDMVHESERWYAARPDYVARMTERGSRYLFYIVEELH
                     KRNMPTELALLPFIESAYNPQAMSVARASGMWQFVPSTGRDYELKQNIFRDDRRDVLA
                     STRAALDYLQKLHDMFGDWQLALAPYNWGEGSVQKAVARNQKAGLPTDYASLRMPAET
                     RMYLPKLQAVKNIIMRPADFGLQLPELMNHPYFLSVPIEHDMDVAIAAQIAGMPLDEF
                     RALNPQMNRPVILAAGTPQVLLPYDNANRFVRELPRHRGPLATWTAWTAPKTMKPAEA
                     ARHVGMSEAQLRDVNHIPARMLIKVGSTLLVPRGSSRDADVPSHVADNAVLALAPDAP
                     PLRRVSLRAGRHESVASVARRYRVNPVQVAQWNGVSAKARFAPGQRIVVFVAPARQIH
                     RSAAVRTAAASHKGSSQHHVVAAARSRATQHVARARRARSAPATKHARARSPQMDLAA
                     N"
     gene            complement(6822..7592)
                     /gene="gloB"
                     /locus_tag="VFF72_12570"
     CDS             complement(6822..7592)
                     /gene="gloB"
                     /locus_tag="VFF72_12570"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZV94044.1"
                     /translation="MNLTAVPAFSDNYIWMIDDGRSAIVVDPGEAEPVQAALDARRLA
                     LAAILVTHHHADHVGGVDALRDRLRGPVFGPMHESLPQPCEGHVEGDVVEALGLQFEV
                     IDVPGHTAGHIAFFNRAGAGEPLAFCGDTLFSAGCGKLFEGTPAQMHASLAKLSDLPD
                     ATRVCCGHEYTLQNLRFAQAVEPGNADIAVHAEACKALRIEGKPTLPSAMGRERRINP
                     FLRTAEPAVIDAAVAHGASGRSGSEVLAAIRTWKDDFR"
     gene            7591..>7730
                     /locus_tag="VFF72_12575"
     CDS             7591..>7730
                     /locus_tag="VFF72_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZV94045.1"
                     /translation="MTEGAIIQLPEWLKTPAGAYLLDWEQRHLDQAVADVFGFHALQL
                     GLP"
BASE COUNT         1193 a         2554 c         2750 g         1233 t
ORIGIN      
        1 ggcgtcgaca ccgagacttt cggcagcgcc gtcgctcgac attggggact cggcgacgac
       61 gtgctgcaca tgatccgacg cctgccggcg gaatcgcccg tgcgcaaacc ggacggcgat
      121 gccgatttgc ttcgtcttgt cggcagcgcc gccaacgaga ccgtcgacgc gatcagcgtg
      181 ctcccgtcaa cgcgagtcgg cgcggccctt gcgcacatcg cgcagcgata ttcccgagtc
      241 ctgcggacca cgccgagaat gctcgccgaa gcgatccagg aagcgcgcga tgtggtgcga
      301 aagggcgccg cgatgccggc ggggcggcag atcgcaaagg caacgaccga tccggtagac
      361 gcgctcccgg agccggatgc cgtgccgccg agagacggtg cgaacgcgcc gcatccctga
      421 tcccgatacc agcgacgcgg tttgcccgca aaccttgccg cgtggagcgt aacgggcaac
      481 gcctggccgt cactcgccag ggctgttccg cggatcttcg aggagaacgt gggccgcctg
      541 ccactcggcc cgcgctcggc tatgcgcctg cgatcagcat cccgacactc gcggccgcca
      601 gcgtcaggac gacggcaagg accagccacc agtctccggc cgaggttcct cgagggcggt
      661 tgccgggacc tgtcgaattg gtttcgctga aaggctgcat gcggcgcacg ataggcgccg
      721 ctccaaggct tgcacatccc ccgggatgag gatcagcgcc gcgccagcgt aggcaagcgg
      781 caggagttcc cgcgcggaca tctccatttg cgcaaggctc gttcgacgga tcggctcgcg
      841 cacctggccg cctcgcaagc cacagcccct gcaaatggaa ggtgacgagc ctgaccccgg
      901 cactggtcgg aacagttagg gctgctccgt tcccgatctg acccggttgg ccgtccttcc
      961 atgcggggag gcccgtcgag atggattcta ggcggccggc gccgcgccca cgatgtccct
     1021 cagatgcgaa ggccgagctc gtggccttcg aaagtcacgc ccagcggctc gatgacgact
     1081 tgcttcggtc cggcctcgac cgtacccgcg acccatgcgt cgatgcccgc cttgcgggct
     1141 gcggcggccg tgcgctcggc gtcctcggcg gcgacgaaga cggcaaagcc ggcgcccatg
     1201 ttgaaagtgc cgtaggcgtc gtggtccgac atgcgggctt cctcctggat gaagcgaagc
     1261 acaggcggaa cgtcgggcag ggtgtggatc cgatagacca ggtcggcccg gtgccgcatc
     1321 agctttcgcc agccgtggcc ggtgatgttc gcggcgtagt gcggccgcac gccggccctg
     1381 aagatcgcct ccgtgatcgg cggatagagc acggtcggct cgagcaatgc gtcgccgaaa
     1441 tcgatgggca gcgcgctgcc gctgagcggc gtcatgtagc cgcgcggcaa tcgcgcggcg
     1501 agctgccggg ccaggctcac gccgttggcg tggatgccgc tcgatgcgag caggacgatc
     1561 gcgtcgccga ccgcgaggtg ctcgcccacg cagaggcggt tcttcgggct gacgatgccc
     1621 gtgcaggatg cggccagatc gatgcggccg gattcgacga cgccggctag cgccggcgtc
     1681 tcgccgccgc cccaggcgat gccgcagtgg tcgcacgccg ccttccagcc cgcgaccagc
     1741 gcctgcgacc ggtcgccatc ggcgaaccac tgcgaagccc cggccgccca gtaggcctgc
     1801 acgacaagag gcgtcgcacc gacggtgacg atgtcgttga cggccattgc gatggtgtct
     1861 tgcgcgatgg ccgcatacgc gctcttgccg gtgagctgtt cgaccgcgtc ggcgacaagc
     1921 accttcgagc ccaggcattc gacgatgctg gccaggtaga acggaccgac gtcgacgacg
     1981 taggcagatt cgccccgtga cgcttcgact tcgctgaagc cgcgggcgtc gagatggcgg
     2041 cccgtcgacg cggctgcgcg ctgcgcggcg agcttgagcg gatcgatgac gtcgtaatcg
     2101 acgccggcag cgccgtaact gagcggggaa gggtctgccg gatcggtcgc catcgcggtt
     2161 gacatcgttt cgagcgcacg gtcgatgcgc cgcggattat cccgcagcga ccggccgggc
     2221 aggggtggcc ccgtagaatc gtcgacatgc cttccacggt gctggcgcgc aaatatcggc
     2281 cgcgcaattt cgaggcgatg gtcgggcaga cccacgtcgt ccaggcgctc gccaatgcgt
     2341 tgacgcagca gcgcctgcat cacgcctatc tcttcaccgg cacccgcggc gtcggcaaga
     2401 cgacggtgtc gcgaatcctc gccaagtcgc tgaattgcac aggtgtcgat ggcctcggag
     2461 gcgtcacggc gcacccctgc ggcgtctgca gcgcgtgccg cgatatcgat ggcggtcgct
     2521 tcatcgacta tgtcgagctc gacgcggcgt cgaaccgcgg cgtcgacgag atcagccagc
     2581 ttctcgatca atcggtctac aagcctgtcg ttggccgctt caaggtgtac atgatcgacg
     2641 aggtgcacat gttgtcggca cacgccttca acgcgatgct gaagacgctc gaggagccgc
     2701 ccgattacct gaagttcgtc ctggcgacga ccgacccgca gaaagtcccg gcaaccgtcc
     2761 tgtcgcgctg cctgcaattc aacctgcggc cgctggcacc gcagacgatc gcgacgcatc
     2821 tggagtcggt gctggcgcag gagtcggtcg gcttcgacgc ggtcgggctg aggctgatcg
     2881 cccgggcggc gcgcggctcg atgcgcgacg cgctctcgct ggccgaccag gcgattgcct
     2941 acggtggcgg cgccgtcgag gtcgatccgg tgcgtcgcat gctgggctcg gtcgaccgcg
     3001 atcacgcggt tcgcatcctc gacgcgctgg ctcgcgccga cgggcgcgca cttgtcggcg
     3061 ccgtcgatca ccttcgcgaa ctgggcctgt cggcagccgg cacgctggag gaactcgcca
     3121 cgcttctgca ggagatggcg atcgcccagg cggtaccgtc gagcgatccg atcgacgacc
     3181 ccgacgcgcc ggcgatcgtc cgcctggcgg cgctgctcga ggccgatcac acccagctgc
     3241 tctacagcat cacgctgaca ggacgtgccg agttgccgct ggctccggac gaatacagcg
     3301 gcctcgtgat ggtgcttttg cgcatgctcg ccttcgcccc gcgaggcgcc gccgattccg
     3361 cgccgaaagc cggaccggcg ccgatcgcgg aaagcacgtc gccacggaat cgggcacctc
     3421 tggcgacgtc ggcctccgtc gagtcgtcga tggccaacgc ctcggccttg cacaaagaga
     3481 ccgttgcggg aaagcccgaa tcgttgcgtg aggcatcgac cgcaccggcg aacgatgccg
     3541 aggcaacggc ggcgaccgga agcgttgcga acgttccgtc cccggcggag ccaggcgcgc
     3601 cgtcggcatc gcccgcgccg gcaccttcgg atcgcgacgc cgccgatcga tggttcgaga
     3661 tcgtgcagcg catgatcggc gcggcgacga ttggcgcgat ggttcgcgag ctggcgctgc
     3721 agtccgagtg cttcgccatc gtcgacaccg ccgatgagca gctctggagg ctgcgcgtcg
     3781 agcgggacaa cctgcgttcg gccggcctgc tcgaacgact gcagacggca ctgggcgaag
     3841 gcgcgcagcg ccgcgttcgc atcgaggtcg aacccggacg cgcgctcgac tcgccggccg
     3901 agcggctggc cgccgaacgc cggcgacgcc agctcgaggc ggaaaggacg attcacgacg
     3961 atccgctcgt caaggcgctg ctcgcgcagt accacacggc gcgagtcgtt cccggctccg
     4021 tgaagccgct gtgattttcg aggagctcaa acgatgatga agggtcaact ggccggcctg
     4081 atgaaacagg cccaggcgat gcaggacaac ctgaagaagg cgcaggacga gctggcgacc
     4141 atcgaggtcg agggccagtc gggcgccggc ctcgtcaagg tgacgatgac gtgcaagcac
     4201 gatgtcaagc gggtgacgat cgatccgagc ctcgtcggcg acgacaagga catgctcgag
     4261 gacctggtcg ccgcggcgtt caacgacggt gtccgccgcg ccgaggcggt gagccaggag
     4321 aagatgggca agctcaccgc cggcatgccg ctgccgccag gcatgaagct gcccttctga
     4381 tggcgccgcg ccttcctggc ccgcggcccg gcgtgcagtt gcagtcggtc tcgaagcggc
     4441 gcggcgccgg ttctgcaagt cctctgtttc cgttcgaaaa gcgcgtcgac cttggccgct
     4501 gaagcctcgc tcgcggacct cgtcgaggcg ctgcgccgct tgccgggagt gggcgtcaag
     4561 tcggcgcaga ggatggcgta tcacctcctg cagcacgatc gcgatggcgc gcggcgaatc
     4621 gccgagtcgt tgcagcgggc ggtggcacgg gtgcagcatt gccagctttg ccatacgttc
     4681 acggaggacg tggtttgcag catctgccgc gacccgtcgc gcgaccggcg ccagctcgcc
     4741 gtcgtcgaga cgccggccga ccaggcagcg ctcgagcgaa gcggcagttt tcgaggccac
     4801 tatttcgtgc tgatgggccg gctcagcccg ctcgacggca tcggtccttc cgagatcggt
     4861 gcccgcgagt tgctcgatcg cgccggcgat ggtgtcgtcg aggaggtcat cgtcgccacc
     4921 aacttcaccg cagagggcga ggcgaccgcg cacgtgcttg ccgaagcctt gcaatcgcgc
     4981 ggtgtccggg tcacgcggct ggcgcgtggc gtgccgatcg gcagcgagct cgagtacgtc
     5041 gatctgggca cgatcgccca cgccttcgtc gatcggcgct gaagcgccgg cgagcgatct
     5101 ggagccgcgc cggcgcgcgc gtcagttcgc ggccaggtcc atctgcgggc tccgggcgcg
     5161 ggcgtgcttc gttgccgggg ctgaacgtgc gcgacgggcc cgtgcgacgt gctgcgtcgc
     5221 gcggctgcgt gcggcagcga ccacgtggtg ctgcgacgaa cccttgtgac tggccgctgc
     5281 ggtgcgaacg gcggcgcttc ggtggatctg gcgggcgggc gcgacgaaga ccacgatccg
     5341 ctgccccggc gcgaacctgg ctttggcgct gacgccgttc cattgcgcga cctgcaccgg
     5401 gttgacgcga taccttctcg cgacggaagc gacgctctcg tggcgaccgg cacgcagcga
     5461 caccctgcgc aggggcggcg cgtcgggggc gagggcgagc acggcgttgt cggcaacgtg
     5521 gctcggcaca tcggcatcgc gcgagctgcc gcgcggcacg agcagcgtcg agccgacctt
     5581 gatcagcatg cgcgccggga tgtggttgac gtcgcgcagt tgcgcttcgc tcatgccgac
     5641 atgcctggct gcttcggcag gcttcatggt cttgggcgcg gtccaggccg tccaggtggc
     5701 gagcgggccc cgatggcggg gcagctcgcg gacgaaccgg ttggcgttgt cgtaaggcag
     5761 caggacctgc ggcgtgccgg cggccaggat cacgggccgg ttcatctgcg gattgagggc
     5821 ccggaattcg tcgagcggca tgccggcgat ctgcgcggca atcgcgacgt ccatgtcgtg
     5881 ctcgatcggc acgctcagga agtaagggtg gttcatgagt tccggcagct gcaggccgaa
     5941 gtcggccggc cgcatgatga tgttcttcac cgcctgcagc ttgggcaggt acatccgcgt
     6001 ctcggccggc atgcgcaggc tcgcatagtc ggtcggcagg ccggccttct ggttgcgcgc
     6061 cacggccttc tggacgctgc cttcgcccca gttgtacggc gcgagcgcca gctgccagtc
     6121 gccgaacatg tcgtggagct tttgcaggta gtcgagcgcg gcccgggtcg aggcgaggac
     6181 atcgcggcgg tcgtcacgaa agatgttctg cttcagctcg tagtcgcggc cggtcgacgg
     6241 cacgaattgc cacatccccg aggcgcgggc caccgacatc gcctgcgggt tgtaggcgct
     6301 ctcgatgaag ggcagcagcg ccagctcggt cggcatgttg cgcttgtgca gttcctcgac
     6361 gatgtagaag agatagcggc tgccgcgctc ggtcatgcgg gcgacgtagt cggggcgtgc
     6421 cgcgtaccag cgttcgctct cgtggaccat gtcgttgtcg atgtccggga tcgcgaagcc
     6481 cttgcgaacg cgctgccaga gatcgacgcg ggcgctgcgg tcgtcgatgt tgatgtgcac
     6541 gtccggacgt tccggatcga tgggcaccgg cgcggcgacg ctttcgtcgc cctgcgaagc
     6601 ggccgtctct gaagccgggg ttgcactcgc gccggcgtcg ggagggcgcg gaagcgggga
     6661 tggagtcgcg gcggcgaccg cgacgtctgt gcccgcgtcg gcgcgtgccg aggcattggg
     6721 gtgggtcgac aaaggcgccg agacgcagcc ggccagtagg aggaggagcg tcgcgagcgc
     6781 gaggaagcgc tgcgggccgg ccgggagtct cgggagtgtc gtcatcggaa gtcgtctttc
     6841 caggtgcgga tcgcggcgag cacctcgctg ccgcttcttc cactggcgcc gtgcgcgacg
     6901 gcggcgtcga tcacggccgg ctcggccgtg cgcaggaacg gattgatgcg ccgttcccgg
     6961 cccatcgccg aaggcaaggt cggcttgccc tcgatgcgca gggccttgca ggcttcggcg
     7021 tgcacggcga tgtcggcgtt gccgggctcg accgcttgcg cgaagcgcag gttctgcaat
     7081 gtgtattcgt ggccgcaaca cacgcgcgtc gcgtcgggaa gatccgacag cttggcgagc
     7141 gaggcgtgca tctgcgccgg cgtgccttcg aagagcttgc cgcagccagc ggaaaagagc
     7201 gtgtcgccgc aaaaggcgag cggctcgcct gcgccggcgc gattgaaaaa ggcaatgtgg
     7261 ccggccgtgt gccccggcac gtcgatcact tcgaattgca ggccgagcgc ctcgacgaca
     7321 tcgccttcga catgaccctc gcagggctgc ggcaggctct cgtgcatcgg cccgaagaca
     7381 gggccgcgca ggcgatcgcg aagggcgtcg acgccaccga cgtgatcggc gtggtgatgg
     7441 gtcactagaa tcgccgcgag cgcgagtcgc ctggcatcga gcgcggcctg caccggctcc
     7501 gcttcccccg gatcgaccac gatggcgctg cggccatcgt cgatcatcca gatgtagttg
     7561 tcgctgaagg caggtacggc ggtcaagttc atgacggaag gcgcgatcat acagttgccc
     7621 gagtggctga agactcctgc cggcgcctac ctgctggact gggagcagcg ccatctcgac
     7681 caggctgtcg ccgacgtgtt cggttttcac gcgctgcagc tgggtcttcc
//
LOCUS       DASRWS010000658         4281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_263465, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000658 DASRWS010000000
VERSION     DASRWS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263465"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFF72_12580"
     CDS             complement(<1..801)
                     /locus_tag="VFF72_12580"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZV94046.1"
                     /translation="MTRVAAADDRTGEGMGRGLGENAPGRGDDCVVYVACADSRAIAV
                     LGLDGRSGALTLRQQVDAGGKVMPLAVSPDRRVLHASIRSEPFAVASFSIDPDDGHLA
                     PLGRAALPASCCWIGTDRSGRYLLSASYGSSLVAVSPVDAAGRVGTALQVSGTPANAH
                     SIRADPANRFLLVACLGGGVVLCCRFDGASGGFAVVDGSTFHARPGAGPRHFVFHPSA
                     PLVYLINELDATVDVLGWDAAKGTLLPLQTVATLPPGFAGKPWAADLHL"
     gene            complement(838..2121)
                     /locus_tag="VFF72_12585"
     CDS             complement(838..2121)
                     /locus_tag="VFF72_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZV94047.1"
                     /translation="MDALVLIGSFFVLMMIGVPIAYSLGLASLFAALWIGLPLDAVMI
                     QIASGVAKFSLLAIPFFVLAGAIMAEGGMARRLVAFASVLVGFVRGGLSLVNILASTF
                     FAGISGSAVADTASIGSALIPEMERKGYPRDFATAVTVSGSVQAIVLPPSHNSVIYSL
                     AAGGSVSIAALFMAGVVPGVLLGLTLSVLCLVKARQHNYPKGERIPLRQALRICAEAM
                     WGVVTMVIILGGILSGVFTANESASIAVLWAFFVTMFIYRDYKWRDVPRLVHRTVKTV
                     TIVMILIGFAASFGYLMTLMQIPLKATQFFASVSHDKLVILALINVMLLVLGCLMDMS
                     PLILICTPVLLPVVKSLGVDPLHFGMIMLLNLGIGLITPPVGTVLFVGSAIGGVRIGT
                     LVKAMTPFFVAMFVVLMMVTYIPMLSLWLPRLLHL"
     gene            complement(2209..2784)
                     /locus_tag="VFF72_12590"
     CDS             complement(2209..2784)
                     /locus_tag="VFF72_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZV94048.1"
                     /translation="MSGAGDSTTVPPPRPTRSPIPRWLDAVYLVSVWSAGVSILAMSI
                     IIPWGVFTRYVLGSGSAWPEPIAILLMMVFTFIGAAAAYRAGSHIAVTMLSDFLPPLL
                     RRAAAVASDLLMLAAAVFVVLYGGELCVATWGQSVAELPWLPVGATYLPLPIGGVLTL
                     AFVLERMVFGSQAGRPLVRYELAAAAAESVD"
     gene            complement(2781..3779)
                     /locus_tag="VFF72_12595"
     CDS             complement(2781..3779)
                     /locus_tag="VFF72_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZV94049.1"
                     /translation="MTHRRQFAALGAGFAVTAALGLAPSLVAAQATLTLRAADVHPPG
                     YPNVVAIEHMGQKLEAATHGRIKLKMFPGGVLGGEKEMIEQTQVGAIDILRTSLGPLG
                     PVVPDVNVFNMPFVFRNEAHMRDVIDGPIGDEMLAKITASPAKLVALGWMDGGSRSIY
                     TKKPVRSMADLKGMKIRMIGNPLFVDTMNAMGGNGISMGYGEVFSALQTGVIDGAENN
                     PPSYFTANHFAAGAKYYTLTNHLIIPEIFVMSKVTWDKLGPEDKVLVRKLSREAQFEQ
                     RKLWDASVGEYVAKLKAAGVEFIPIDNKPFYDATAGVRAKYGAPYADLMKRIEATK"
     gene            complement(3814..>4281)
                     /locus_tag="VFF72_12600"
     CDS             complement(3814..>4281)
                     /locus_tag="VFF72_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV94050.1"
                     /translation="SSNHVTGFYRQDEVIGPDAPMRPDGHYGISKAFGENLSRFYFDR
                     YGIETVCLRIGSSFPEPKDRRMLATWLSFDDLERLVVAALGAPVVGHSVVYGVSDNLT
                     TWWDNPSAGPLGFRPQDSSEPFRAAVEARQPHLDPADPAVRFQGGGFVTRGPF"
BASE COUNT          756 a         1389 c         1552 g          584 t
ORIGIN      
        1 caggtggaga tcggccgccc acggcttgcc ggcgaagccg ggcggcaacg tggcgacggt
       61 ctgcagcggc aggagcgtgc ccttcgccgc atcccagccc agcacgtcga ccgtcgcgtc
      121 gagctcgttg atgaggtaga cgagaggcgc cgacggatgg aagacgaagt ggcgcggccc
      181 ggcgccgggt cgcgcatgga acgtcgaccc gtcgacgacg gcaaagccac cgctcgcgcc
      241 gtcgaagcga cagcaaagga cgacgccgcc gcccaggcac gcgacgagaa gaaagcggtt
      301 ggccggatcg gcccggatcg aatgcgcgtt cgccggcgtg ccggacacct gcagcgccgt
      361 gccgacgcgg ccagcggcat cgacggggct gacggcgacg aggctgctgc cgtacgaggc
      421 gctcagcagg tagcggccgc tgcggtcggt gccgatccag cagcaactgg cgggcagcgc
      481 cgctcggccg agcggcgcga gatggccgtc gtccggatcg atcgaaaagc tggcgacggc
      541 gaacggctcg gagcggatcg acgcgtgcag cacgcgccgg tcggggctga ccgcgagcgg
      601 catgaccttg cctcccgcgt cgacctgctg gcgcagcgtc agcgcgccgc tgcgcccatc
      661 gaggccgagc acggcgatgg cgcggctgtc ggcgcaggcc acgtagacca cgcagtcgtc
      721 gccgcggccg ggcgcgtttt cgccgaggcc gcggcccatg ccttcgccgg ttcgatcgtc
      781 ggcagcggcc acgcgcgtca tcggcgaatc atgcgcccgg cgagccggcg ccgcgcgtca
      841 caggtgcagc agccgcggca gccacagcga cagcatcggg atgtaagtga ccatcatcag
      901 gacgacgaac atggcgacga aaaacggcgt catcgccttc acgagcgtgc cgattcgcac
      961 gccgccgatg gcgctgccga cgaagagcac cgtgcccacc ggcggcgtga tcaggccgat
     1021 gccgaggttg agcagcatga tcatgccgaa gtggagcgga tcgacgccga gcgacttgac
     1081 gaccggcagc agcaccggcg tgcagatgag gatgagcggc gacatgtcca tcaggcagcc
     1141 gagcaccagc agcatgacgt tgatgagggc gaggatgacc aacttgtcgt gcgagacgct
     1201 ggcgaagaac tgggtcgcct tgagcgggat ctgcatcagc gtcatcaggt agccgaagct
     1261 cgccgcgaag ccgatcagga tcatgacgat ggtcaccgtc ttgacggtgc gatggacgag
     1321 ccgcggcacg tcgcgccact tgtagtcgcg gtagatgaac atcgtcacga agaaggccca
     1381 gaggacggcg atcgacgccg actcgttggc ggtgaagacg cccgagagaa tgccgccgag
     1441 gatgatgacc atcgtcacca cgccccacat cgcctcggcg cagatcctga gcgcctggcg
     1501 cagcggaatg cgttcgccct tggggtagtt gtgctgcctg gccttgacga ggcagagcac
     1561 cgacagcgtc aggccgagca ggacgccggg cacgacgccg gccatgaaga gcgccgcgat
     1621 cgagacgctg ccgccggcgg cgagcgaata gatcaccgag ttgtgactgg gcggcaggac
     1681 gatcgcctgc accgagccgc tcaccgtcac cgccgtcgcg aaatcgcgcg ggtagccctt
     1741 tctttccatc tcggggatga gcgccgagcc gatcgaggcc gtgtcggcga cggccgagcc
     1801 cgagatgccg gcaaagaacg tcgaggcgag gatgttgacg agcgacaggc cgccgcgcac
     1861 gaatccgacc aggacgctgg cgaaggcgac gaggcgccgg gccatgccgc cctcggccat
     1921 gatggcgccg gcgagcacga agaagggaat cgccagcagc gagaacttgg cgacgcccga
     1981 ggcgatctgg atcatcaccg cgtcgagcgg caggccgatc cacagcgccg cgaacagcga
     2041 ggcgaggccg agcgagtagg cgatcggcac gccgatcatc atcaggacga agaagctgcc
     2101 gatgagaacg agcgcgtcca tcagcgagcg ccccgtcgat gcggacgggc ccgcgtcggc
     2161 accggggccg gcgggaggcg cgcaatgccg gctgcctgcg gcgccatgtc agtcgacgct
     2221 ttccgcggcg gccgcagcca gctcgtagcg caccagcggc cggccggcct gcgagccgaa
     2281 gaccatccgc tcgaggacaa aggcgagcgt caggacgccg ccgatcggca gcggcagata
     2341 ggtcgcgccg accggcagcc agggcagctc ggcgaccgac tgtccccacg tcgccacgca
     2401 cagctcgccg ccgtagagga cgacgaagac cgcggcggcc agcatcagca ggtcgctcgc
     2461 gacggcggcg gcccgacgca gcagcggcgg cagaaagtcg ctcagcatcg tgacggcgat
     2521 gtgcgagccg gcgcgatagg cggcggcggc gccgatgaag gtgaagacca tcatcagcag
     2581 gatggcgatc ggctcgggcc aggccgagcc cgagccgagg acgtagcgcg tgaacacgcc
     2641 ccacggaatg atgatcgaca tggcgaggat cgagacaccc gccgaccaga cgctgacgag
     2701 gtagacggcg tcgagccagc gcgggatcgg cgagcgcgtc ggccgcggcg gtggaaccgt
     2761 cgtcgagtcg ccggcgccgc tcatttcgtc gcttcgatcc gcttcatgag atcggcatag
     2821 ggcgcgccgt acttggcccg cacgccggcc gtcgcgtcgt agaacggctt gttgtcgatc
     2881 gggatgaact cgacgccggc cgccttcagc ttggcgacgt attcgccgac actcgcgtcc
     2941 cagagctttc gctgctcgaa ctgcgcctcg cgcgataact tcctcaccag caccttgtcc
     3001 tcggggccga gcttgtccca ggtgaccttc gacatgacga agatctcggg aatgatgaga
     3061 tggttcgtca gggtgtagta cttggcgccg gcggcgaagt ggttggccgt gaagtagctc
     3121 ggcgggttgt tttcggcgcc gtcgatgacg ccggtctgca gcgcgctgaa gacctcgccg
     3181 tagcccatcg agatgccgtt gccgcccatc gcgttcatcg tgtcgacgaa gagcgggttg
     3241 ccgatcatgc ggatcttcat gcccttcagg tcggccatcg agcgcaccgg cttcttggtg
     3301 tagatgctgc gcgagccgcc atccatccag ccgagcgcga cgagcttggc cggcgaggcg
     3361 gtgatcttgg cgagcatctc gtcgccgatc ggcccgtcga tcacgtcgcg catgtgcgcc
     3421 tcgttgcgaa agacgaacgg catgttgaag acgttgacgt cgggcacgac cgggccgagg
     3481 ggcccgagcg aagtgcgcag gatgtcgatc gcgccgacct gcgtctgctc gatcatttcc
     3541 ttctcgccgc cgagcacgcc gcccgggaac atcttgagct tgatgcggcc gtgcgtggcg
     3601 gcctcgagct tttgccccat gtgctcgatg gcgacgacat tcggataacc cggcggatgg
     3661 acgtcggcgg cgcgaagggt gagcgtcgcc tgcgccgcga cgaggctcgg cgcgaggccg
     3721 agcgcggcgg tgacggcgaa gccggcgccg agggcggcga actgtctgcg atgggtcatg
     3781 gtcgggtctc ctctgcgccg ggggcgacgc gtgtcaaaag gggccgcggg tgacgaaacc
     3841 accgccctgg aagcggacgg cgggatcggc cgggtcgaga tgcggttgtc gggcctcgac
     3901 tgccgcgcga aagggctcgg agctgtcctg cggacgaaag ccgagcgggc ctgcgctcgg
     3961 gttgtcccac cacgtcgtca ggttgtcgga gacgccgtag acgacgctgt ggccgacgac
     4021 cggcgcgccg agcgcggcga cgacgaggcg ctcgaggtcg tcgaagctca gccacgtcgc
     4081 gagcatccgt cgatccttcg gctcgggaaa cgacgagccg atgcgcaggc acaccgtctc
     4141 gatcccgtag cggtcgaaat agaagcgcga gaggttctcg ccgaacgcct tgctgatgcc
     4201 gtagtggccg tcgggtcgca tcggcgcatc ggggccgatc acctcgtcct gccgatagaa
     4261 acccgtgacg tggttggagc t
//
LOCUS       DASRWS010000659         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4278692, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000659 DASRWS010000000
VERSION     DASRWS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4278692"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFF72_12605"
     CDS             complement(<1..197)
                     /locus_tag="VFF72_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZV94051.1"
                     /translation="MRYFVTGATGFIGKRLVKALLARRGASVHFLLRPESAGKLDELL
                     DFWGVAGSRARAVPVYGDLRA"
     gene            complement(418..702)
                     /locus_tag="VFF72_12610"
     CDS             complement(418..702)
                     /locus_tag="VFF72_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94052.1"
                     /translation="MQELQTNYRGQCGEDEANARRRLDEVRRQKRDARVSEANAVDAQ
                     RERDIISREQCDEMYRIVHRRRQTLAAMTPGERADFDRFESNWKARCRPG"
     gene            1201..1737
                     /locus_tag="VFF72_12615"
     CDS             1201..1737
                     /locus_tag="VFF72_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZV94053.1"
                     /translation="MVKKLKAMADKQKRQASAAGLLDSELAKTVRESAQQIWLAGMGA
                     FAKAQAEGKQRFEALVKEGASLQKKTQNVAEEKLGEVTSRMTSMADDVTSRAGQHWDK
                     LESIFEERTAKALKKLGVPSSRDVQALQDKIDALSAQVAGRRQASAAAAKKAGGAKKA
                     AGKTTRRSAAKKSARSAA"
     gene            complement(1823..>3121)
                     /locus_tag="VFF72_12620"
     CDS             complement(1823..>3121)
                     /locus_tag="VFF72_12620"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZV94054.1"
                     /translation="AAGGEPLDDAARANLREMRRVCDRANALPAELVEAKSLATSRCE
                     QAWQTQRPANDWAGFAVNLREVVRVMRDEAKCLSDASGMAPYDALLDRYEPGMTDAVV
                     SGLFEQLKTWLPQLVRDIRHRQSGDVAIRPRGPFPKAAQRALSLDVMTMLGFDFEAGR
                     LDESAHPFNGGVPEDTRLTTRYRDDDFVQSLMSTIHETGHARYEQNLPRSLLGQPVGR
                     ARSMAVHESQSLSFEMQLGRSRGFVDRLPPLLRRHLGDDPALDAANLHLLLTRVEPGL
                     IRVDADEATYPLHVILRHGIEKRLVDGDVEVQDIPALWDDGMASLLGLDTRGNFSDGC
                     MQDVHWAAGLFGYFPCYTLGAMQAAQWFAAMRKVTPGLDARIAAGDLAPVFDWLRENI
                     WLQASRWTTTELSKRASGETLDPIHFRRHLEARYLAGAGR"
BASE COUNT          536 a         1038 c         1074 g          473 t
ORIGIN      
        1 ttggccctca ggtcgccgta gacgggaacc gctcgcgccc ggctcccggc gacaccccag
       61 aagtcgagca gttcgtcgag cttgccggca ctctcgggac gaagcaggaa atggacgctg
      121 gccccgcgcc gggccagaag cgccttgacc agccgcttgc cgatgaagcc ggtcgccccg
      181 gtgacgaagt agcgcatgcc tgtcctttca gtgcgcggcg ccacgtcgca cggcgccgtc
      241 acgggaattc ttgtgcaaac gatgcgggcg acgcgcgggg caaaccccga cgctccgtcg
      301 cccacgcgtc cgccacacgg acaagctgcc tcgccgacct ggatcgtctc gcagccggac
      361 gcacgtcgag cgcgcatcgc tggcgcacgt ttgccgcggt cgcagggaag gtacccgtca
      421 gcccggacgg cagcgtgcct tccagttcga ttcgaatcga tcgaagtcgg cacgctctcc
      481 tggcgtcatg gccgcgaggg tctgacggcg gcgatggacg atcctgtaca tctcgtcgca
      541 ttgctcgcgg gaaatgatgt cgcgctcacg ctgcgcatcg accgcattgg cctcgctgac
      601 gcgtgcgtcg cgcttctgac gtcggacttc gtccagcctt ctgcgggcat tggcttcatc
      661 ttctccgcat tgcccacggt agttcgtctg cagctcctgc atggcgcgcg aaccgagacc
      721 gcgactcggc ccgacgcgaa ggccctcgtt gagttctgcg cagagcggag aaagatactc
      781 gagatagtcg accgccttgc cggcggggcg cacgcgtgaa tacgctgcgg gccggtcgcg
      841 caagggtcct gcggcgccca gcaccgtcgt tggtcgaccg ccgcacggac ggtccgagag
      901 atacgcgcgg ccgtccgatg agcatcggta cagcgtctgc gaagtcgcca ctggcgacac
      961 cgccccgagc acaatcagcg cggcagctgc cgcaatggcg ttgcgaccca ttggccctcc
     1021 cgttccaatg caggagtcta cgggcgcaac cgtcggattc gcagggctcg atgggggggt
     1081 ccgcatcgcc acgatcgacg acggcccagc cggcgattag ggtgtacgcc caagacttcc
     1141 tccgtagact ggccgccaac gctgcgaatc gcggcgcaac acaagatcgg agaatcagcg
     1201 atggtcaaga aactcaaggc gatggcggac aagcagaagc ggcaggcatc ggcggcgggg
     1261 ctgctcgaca gcgagctggc caagacggtc agggaatcgg cacagcagat ctggctcgcc
     1321 ggcatgggcg cgttcgccaa ggcacaggcc gagggcaagc agcgcttcga ggcgctggtc
     1381 aaggaaggcg cttcgctgca gaagaagacg caaaacgtgg ccgaggaaaa gctcggtgaa
     1441 gtgaccagcc gcatgacgag catggccgac gacgtcacgt cgagggcggg ccagcattgg
     1501 gacaagctcg aatcgatctt cgaggagcgc accgccaagg cgctgaagaa gctcggcgtt
     1561 ccgtcatcgc gggacgtgca ggcgctgcag gacaagatcg acgcgctctc ggcacaagtt
     1621 gccgggcgcc gccaggcgag tgctgccgcc gcgaagaagg caggcggcgc aaagaaggcc
     1681 gccgggaaga cgacacgcag atcggcagcc aagaagtcgg cgcgctccgc cgcctgaaga
     1741 agcgattcga cgcccgcggc ggcaacctcg gggggacgcc gcggcgatcc ttcctttccg
     1801 gacgggttgc gctgcgccgc cgtcagcgac cggcgcctgc gaggtagcgc gcttcgagat
     1861 gtcgcctgaa atggatcggg tcgagcgtct ctccgctggc gcgcttcgaa agttccgtcg
     1921 tcgtccagcg gctggcctgc agccagatgt tctcgcgcag ccagtcgaag accggcgcga
     1981 ggtcacccgc ggcgatgcgg gcgtcgaggc ctggcgtgac ctttcgcatc gcggcgaacc
     2041 actgcgccgc ctgcatggcg cccagcgtgt agcaggggaa gtatccgaac agtccggccg
     2101 cccaatggac gtcctgcatg cagccgtcgc tgaagttgcc gcgcgtgtcg aggccgagga
     2161 gcgacgccat cccatcgtcc cagagcgccg gaatgtcctg cacctcgacg tccccgtcga
     2221 cgaggcgctt ttcgatgccg tgtcgcagga tcacatgcag cgggtaggtg gcctcgtcgg
     2281 catcgactcg gatcaggccc ggctcgaccc gggtgagcaa cagatggagg ttggccgcgt
     2341 ccagggccgg gtcatcgccc agatggcgcc tcagcaaggg cgggagtcgg tcgacgaagc
     2401 ccctgctgcg acccagctgc atctcgaacg acaggctctg gctttcgtgc accgccatcg
     2461 agcgggcgcg gccgaccggc tggccgagca agcttcgagg caggttctgc tcgtagcggg
     2521 cgtgccccgt ctcgtggatg gtgctcatca ggctttgcac gaaatcgtca tcgcgatagc
     2581 gggtcgtgag gcgcgtgtct tcggggacgc cgccgttgaa tggatgcgcg ctctcatcga
     2641 gtcgaccggc ctcgaaatcg aagccgagca tggtcatgac gtcgaggctg agggcacgct
     2701 gcgcggcctt cgggaaggga ccgcgaggcc ggatggccac gtcgcccgac tgtcgatggc
     2761 ggatgtcgcg cacgagttgc ggcaaccacg tcttcagctg ctcgaagagg ccgctcacga
     2821 cggcatccgt catccccggc tcgtaacgat cgagcagcgc gtcgtacgga gccatgccgc
     2881 tggcgtcgga caggcacttc gcctcgtcgc gcatcacgcg cacgacttcg cgcaggttga
     2941 cggcgaagcc cgcccagtcg ttggccggcc gctgcgtctg ccacgcctgc tcgcaacgcg
     3001 acgtggcgag agacttcgcc tcgacgagct cggcgggcaa cgcattcgcg cggtcgcaga
     3061 cgcgacgcat ctcgcgcagg ttggctctgg ccgcatcgtc gagcggctcg ccgccggccg
     3121 c
//
LOCUS       DASRWS010000660         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2863447, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000660 DASRWS010000000
VERSION     DASRWS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2863447"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFF72_12625"
     CDS             complement(<1..401)
                     /locus_tag="VFF72_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94055.1"
                     /translation="MSLKRVFGLRWRMTAALLAVSALAIAVAAVSLLLPLDVLLRRDA
                     VHSLAAEARTARGALTALPPGELRAGSPQLDRVVRRLGQQTAADVVVLDKDGRILSST
                     DVDPRERYGDVRVALRQDRMVKGSADVPGTDR"
     gene            complement(398..1108)
                     /locus_tag="VFF72_12630"
     CDS             complement(398..1108)
                     /locus_tag="VFF72_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZV94056.1"
                     /translation="MVAASILVAEDQADIRELLVMNLRSAGYDVSAVADGAAALASQS
                     ERSSDLLVLDLMMPGLDGLEVCKALRARGRSTPILMLTAKSTELDRVLGLELGADDYL
                     TKPFSLAELLARVKALLRRADLLRAAQTGTGGDGHVIRNGGLEILPAKRQARFLGRQL
                     EFTALEFDLLLYFASHPGHVFSRSQLLDAVWGYTHDGYEHTVTTHINRLRSKLEADPL
                     RPELILTVRGVGYRLHSP"
     gene            1400..1885
                     /locus_tag="VFF72_12635"
     CDS             1400..1885
                     /locus_tag="VFF72_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZV94057.1"
                     /translation="MAPAIADVVAHRAYLVRFATRKLRDPSLAEDAVHDVFEAVLSGR
                     ATFGGRAALRSWLTAVLKHKIVDTLRRSASHESLEGEDGEDRRDLVSPLAGPEHIAEQ
                     RQRLASALAGIDALPPGLRDAMRLRVVDDRPTEEVCSALSISEENLFVRVHRARRQLL
                     S"
     gene            complement(1919..2266)
                     /locus_tag="VFF72_12640"
     CDS             complement(1919..2266)
                     /locus_tag="VFF72_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZV94058.1"
                     /translation="MSPPGLRVACLCAEWCGTCRDYRATFEALAKRFAGRADFEWVDI
                     EDESDALGDPDIETFPTLLMAEGDRAVFYGTVTPQMQSAERLIEAALAGKLGPPDPAA
                     ESLVASLRGRSRS"
     gene            complement(2263..>2562)
                     /locus_tag="VFF72_12645"
     CDS             complement(2263..>2562)
                     /locus_tag="VFF72_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94059.1"
                     /translation="HAVAKVQLDADGRVTGVEWGPVNTETNEWHTEPRVTGVEEVVAA
                     LHRGEDVFALFPGPHGLEPGRRFRVVSYDNGWETVNLDGPPTHEHEIHDMLHVGA"
BASE COUNT          368 a          921 c          884 g          389 t
ORIGIN      
        1 cggtcggtgc ccggcacgtc ggccgacccc ttgaccatcc ggtcctggcg cagggcgacc
       61 cgcacgtcgc cgtagcgctc gcgcgggtcg acgtccgtgg aggacaggat gcggccatcc
      121 ttgtcgagca ccacgacgtc cgcggccgtc tgctgcccga ggcgccgcac gacgcggtcg
      181 agctgcgggg agccggcgcg cagctcgccc ggcggcaggg ccgtcagcgc gccgcgggcg
      241 gtgcgcgcct cggcggccag cgagtgcacc gcgtcgcggc gcagcagcac gtccagcggc
      301 agcagcagcg acacggcggc gacggcgatg gcgagcgcgc tcaccgccag cagcgcggcc
      361 gtcatccgcc accggagacc gaacacgcgc ttcaggctca cgggctgtgc aggcgatacc
      421 cgacgccgcg cacggtgagg atcagctccg gccgcaacgg gtcggcctcg agcttgctgc
      481 gcagccggtt gatgtgcgtg gtgacggtgt gctcgtagcc gtcgtgggtg tagccccaga
      541 ccgcgtcgag aagctgactg cgcgagaaca cgtgcccggg gtggctggcg aagtagagca
      601 gcaggtcgaa ctcgagcgcc gtgaattcga gctgccgtcc gaggaagcgg gcctgccgtt
      661 tcgccggcag gatctccagc ccgccgttgc ggatgacatg accgtcgccg ccggtgccgg
      721 tctgagccgc gcgcaacagg tcggctcggc gcagcagcgc cttgacgcgg gccagcagct
      781 cggccagcga aaacggcttc gtcagatagt cgtcggcgcc gagttcgagc ccgagcacgc
      841 ggtcgagctc ggtcgatttc gccgtcagca tcaggatcgg cgtgctgcgt ccgcgagctc
      901 gcagcgcctt gcagacctcg agcccgtcga ggcccggcat catcaggtcg agcaccagca
      961 ggtcgctcga ccgttcgctc tggctggcca gcgcggcagc gccgtcggcc accgcggaca
     1021 cgtcgtagcc ggcactgcgc aggttcatca ccagcagttc gcggatgtcg gcctggtctt
     1081 cggcgacgag gatggaagcg gcaaccatgg tcgattgtct cgtcggcgag ccatctctta
     1141 caacgatgtg atcctgccgt gatctgccgc gaggtggccg cgagcgacgg cgtcgacagt
     1201 gacgcatcgc cgctccggac tctcccgcat gcacaccgcc cacgccgtcc gcgccgactc
     1261 gacccggcct ctttcgattc gtcgctcgag cgtcacgaag tcggccgttc cttcgcctgc
     1321 gcacccgcgt ggtgccggac ggccgactgc ggttctgccg gattcgaatg tgacacgcac
     1381 ggctccactc gctgcatccg tggcgccggc catcgccgat gtcgtggccc atcgcgccta
     1441 cttggtccgc tttgcgacgc gaaagcttcg cgacccgtcg ctggccgagg acgccgtgca
     1501 cgacgtcttc gaagcggtgc tttcgggccg tgcgacgttc gggggtcggg cggcgcttcg
     1561 ctcctggctg acggcggtgt tgaagcacaa gatcgtcgac accttgcgcc gatccgccag
     1621 tcacgagagc ctcgaaggcg aagacggcga ggaccgccgc gatctcgtca gcccgctggc
     1681 cgggcccgag cacatcgccg agcagcgaca gcgcctggca agcgcccttg ccgggatcga
     1741 cgcgctgccg cccggactgc gcgacgcgat gcgcctgcgc gtcgtcgacg accggccgac
     1801 cgaggaggta tgcagcgcgc tgtcgatcag cgaggaaaac cttttcgtgc gcgtgcatcg
     1861 tgcgcgccgt cagttgctgt cctgacctcg ctccggcgtc ttgtcgccgg tgcgaaggct
     1921 acgatcggct tcggccgcgc agcgaggcga caagcgactc ggcggccggg tccgggggcc
     1981 cgagcttgcc ggccagcgcg gcctcgatca gtcgctcggc cgattgcatc tgcggcgtca
     2041 ccgttccgta gaaaaccgca cggtcaccct cggccatcag cagggtcggg aacgtctcga
     2101 tgtccggatc gccgagcgcg tcggattcgt cctcgatgtc gacccactcg aaatcggcac
     2161 ggcccgcaaa ccgcttcgcc agcgcctcga acgtcgcccg gtagtcgcgg catgtcccgc
     2221 accactcggc acacaggcag gcgacgcgca ggccgggcgg gctcacgcgc ccacgtgcag
     2281 catgtcgtgg atctcgtgct cgtgcgtggg cggcccgtcc aggttgaccg tctcccagcc
     2341 gttgtcgtag ctgacgaccc gaaagcgccg tccaggctcg aggccgtgcg gccccgggaa
     2401 gagcgcgaac acgtcctcgc cgcgatgcag ggcagcgacg acctcctcga cgcccgtcac
     2461 gcgcggctcg gtgtgccact cgttcgtctc ggtgttcacc ggcccccatt cgacacccgt
     2521 gacccgcccg tctgcatcga gctgcacctt cgctacggca tg
//
LOCUS       DASRWS010000661         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2554527, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000661 DASRWS010000000
VERSION     DASRWS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554527"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFF72_12650"
     CDS             complement(<1..665)
                     /locus_tag="VFF72_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HZV94060.1"
                     /translation="MRLAQVLFTQGFGTRRECAALLHSGLVRIGDSTVDDPELDVATE
                     GLSFFVRGEPWDFHARALLAMNKPAGYECSQKPRHHPSVMSLLPTPLRVRGVQPVGRL
                     DADTTGLLLFTDDGTLIHRLGSPKKHVAKVYDVETAAPVDDAQVEALRKGVVLHDDPR
                     PVGAASCERTGERSLRLTLVEGKYHQVKRMVAAAGNTVVGLCRSRYGRLALPDDLAPG
                     QWR"
     gene            complement(671..2041)
                     /locus_tag="VFF72_12655"
     CDS             complement(671..2041)
                     /locus_tag="VFF72_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZV94061.1"
                     /translation="MSRRRILLAAAASGAAPLVACATLPGDADMPPVVFVPGNGDTAG
                     IWMTTIWRFESNGWPSNRLHAIDMPYPLARDDDSREQPGRSSAAENAAFLAAEVRKVL
                     AATGARKVALVGNSRGGYAIRNFIAEGGAASVSDVVLCGVPNHGVFVNREAALGSEFN
                     GAGAMLSRLNAPRGAEGNEVEPGIRWMTIRSDHEDKYAQPDGVWIGQRGKPTHVTYEG
                     PALRGAENIVIAGVDHRETAFSPKAFAEMYRFIAGRPPATLDIVPASPVILDGIVSGL
                     GLGNTQGNFATNLPLVGATLEVYATNPDTGERIGAPRHRRTIGSDGHWGPFTADGTTR
                     YEFQIAAPGYATTHIYHSPFPRSSDIVNMHPDRMTEADRMAGALVIFNRSRGYFGVPR
                     DHVVLDGKSPPEGIPTGVPGVSSAREKIADAASRTVVGEFDGERIVGHTWPAAQDQLT
                     VLDLTG"
     gene            complement(2078..>2283)
                     /locus_tag="VFF72_12660"
     CDS             complement(2078..>2283)
                     /locus_tag="VFF72_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94062.1"
                     /translation="GDGDVVFALATGGSGQRVHTTVLGALAAEVLADAVLRGVRAAAS
                     LHGAGLPDLPAARDLPMKTTPRS"
BASE COUNT          315 a          768 c          848 g          352 t
ORIGIN      
        1 cagcgccatt gtcccggggc cagatcgtcc ggcagcgcga gccggccgta gcggctccgg
       61 cacaggccga cgacagtatt gccggcggcc gcgaccatgc gcttgacctg gtggtatttg
      121 ccctcgacca gcgtcaggcg cagcgagcgc tctccggtgc gttcgcaact cgccgcgccg
      181 acgggccgcg gatcgtcgtg gaggacgacg ccctttcgca gcgcctcgac ctgcgcgtcg
      241 tcgaccggcg cggcggtctc gacgtcgtag accttggcga cgtgcttctt cggcgaaccg
      301 aggcgatgga tcagcgtgcc gtcgtcggtg aagaggagca ggccggtcgt gtcggcgtcg
      361 aggcgtccca ccggttgcac gccgcgcacc cgcagcggcg tcggcaggag gctcatcacg
      421 ctcgggtggt ggcgaggctt ttgcgagcac tcgtagccgg ccggcttgtt catcgccagg
      481 agggcccggg cgtgaaagtc ccagggctcg ccgcgcacga agaaggaaag accttccgtc
      541 gccacgtcca gctcggggtc gtcgacggtc gaatcgccga tccgcaccag gccgctgtgc
      601 aacagcgcgg cgcactcgcg gcgcgtgccg aagccctgcg tgaacagcac ctgggcgagg
      661 cgcactcggc tcagccggtc aggtcgagga cggtcagctg atcttgcgcc gccggccagg
      721 tgtggccgac gatccgttcg ccgtcgaatt cgccgacgac ggtgcggctc gccgcatcgg
      781 cgatcttctc gcgcgccgac gagacgcccg gcacgcccgt gggaattcct tcgggcgggc
      841 tcttcccgtc gagcacgacg tgatcgcgcg gcacgccgaa gtagccgcgc gagcggttga
      901 agatgacgag tgcgcccgcc atgcgatcgg cttcggtcat gcgatcggga tgcatgttga
      961 cgatgtccga cgagcgcggg aagggcgagt ggtagatgtg cgtcgtcgca tagccgggcg
     1021 ccgcgatctg gaactcgtag cgcgtcgtgc cgtcggcggt gaagggtccc cagtgtccgt
     1081 cgctgccgat ggtgcgtcga tgtcgcggcg caccgatccg ctcgccggtg tcggggttcg
     1141 tcgcgtatac ctcgagcgtc gcgccgacca gcggcaggtt ggtcgcgaag ttgccttgcg
     1201 tgttgcccag gccgaggccg ctgacgatgc cgtcgaggat caccggcgat gccgggacga
     1261 tgtcgagggt cgccggcggc ctgcccgcga tgaagcggta catctcggcg aacgccttcg
     1321 gactgaatgc ggtctcgcga tggtcgacgc cggcgatgac gatgttctcg gcacccctga
     1381 gcgccggccc ttcgtaggtg acgtgcgtcg gcttgccgcg ctggccgatc cagacgccgt
     1441 cgggctgggc gtacttgtcc tcgtggtcgg agcggatggt catccagcgg atcccgggct
     1501 cgacctcgtt gccttcggcg ccgcgcggcg cattgaggcg gctgagcatc gcacccgcgc
     1561 cgttgaactc gctgccgagc gccgcttcgc gattgacgaa cacgccgtgg ttcggcaccc
     1621 cgcacagcac cacgtccgag acggacgcgg cgccgccctc agcgatgaag ttgcggatcg
     1681 cgtagccgcc gcgcgaattg ccgacgagcg caaccttccg ggcgccggtg gcggcgagca
     1741 ccttgcggac ttcggcggcg agaaacgccg cgttctccgc ggccgacgag cggcccggct
     1801 gctcgcgtga atcgtcgtcg cgggccagtg ggtacggcat gtcgatcgcg tgcaggcggt
     1861 tgctcggcca gccgttcgac tcgaaccgcc agatcgtcgt catccagatc ccggcggtgt
     1921 cgccgttgcc cggcacgaag acgacgggtg gcatatcggc gtcgcccggc agcgtcgcgc
     1981 aggcgacgag cggtgcggct ccggaggccg ccgcggcgag gaggattcgg cgtcgggaca
     2041 tcgagcgggg tgaagtcgtc ggcatggcgg ggtccggtca ggagcggggg gtcgtcttca
     2101 ttggcaaatc tcgcgccgcc ggcaggtcgg gcaggccggc gccgtgcagc gaggcggcag
     2161 cgcgcacgcc gcgaagcacg gcgtcggcaa gcacctcggc cgccagcgcg ccgagcaccg
     2221 tcgtgtgcac gcgttggccg ctgccaccgg tggcgagcgc gaagacgacg tcgccgtcgc
     2281 cca
//
LOCUS       DASRWS010000662         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4281046, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000662 DASRWS010000000
VERSION     DASRWS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281046"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..817)
                     /locus_tag="VFF72_12665"
     CDS             complement(11..817)
                     /locus_tag="VFF72_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZV94063.1"
                     /translation="MKKTFTLMRARQRQRRGALAGFVAAASSAMLAGCDRLSGNTGFV
                     TTLKSAQKLSHAAQKIVAPASAMAQEFTQADVAPTFRGNGTLNPGDADYQALRRDGFK
                     DYELMVTGLVRHSASYTLAQLRAMPSRTQITRHDCVEGWSVIGKWKGVQLGRILDQVE
                     PLPEAKYVVFRCFDSMDGPTLDGHDSHYYESIDLDEARHVQTILAYELNDQALPVANG
                     APIRCRVERQLGYKQAKYVRAIELVASFADIRGGKGGYWEDEGYEWYGGI"
     gene            complement(814..1680)
                     /locus_tag="VFF72_12670"
     CDS             complement(814..1680)
                     /locus_tag="VFF72_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZV94064.1"
                     /translation="MSTEAIAASLPATVIAAPARRVYRHRLPVRVMHWINVLCLTILL
                     MSGLNIFNASPSLHWGQSSSGQPWLSLRAENTPHGPVGKTQIGNAEFDTTGVLGLSKV
                     AGSPTARGFPSWATIPGNQWLAMARHWHLFFAWLFVANGVAYVLYTIFSGHLRRDLVP
                     TRVELRHIGRSIVDHLKFRHPTGEAARRYNVLQSLAYLVVIFVLLPLVVLCGMALSPR
                     IDTVFTGWVDLLGGRQSARSLHFIAAFGLLLFVFIHVVEVFIAGVWNEIRSMITGWYV
                     LPKEHAKEKSSP"
     gene            complement(1853..>2786)
                     /locus_tag="VFF72_12675"
     CDS             complement(1853..>2786)
                     /locus_tag="VFF72_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HZV94065.1"
                     /translation="EARFGGVQRYDALLDTATVANLRPFVARAAEEWFRAPLASSLNA
                     AGFSNEWYYTLAVDAADKTVSMGGVGPEIGRNADGLKNIVSLMVATRGRGIGRTDLRR
                     RVAVALASARNVLDNAASHAGDLVKLRQYVERDIVASACQGEVAIEAAPVASEHDLAM
                     LDPLTGAIRHITVSWQSALELRILKSRPRPCGYWLAASESDAVRRLRLLGIDVQQLDE
                     AGEVRGETYREIGRDVRPSDGAARLRVRTSPVLLDMPAGGYYVTMEQPLANLAVAALE
                     PESPAGYAANGVISSVDAEARILDRPQLHTITLP"
BASE COUNT          424 a          972 c          959 g          431 t
ORIGIN      
        1 aacgatctcc tcagatgccg ccgtaccact cgtagccttc gtcttcccag tagccgccct
       61 tgccgccgcg gatgtcggcg aagctcgcga cgagctcgat cgcgcggacg tacttcgcct
      121 gcttgtaacc gagctggcgc tcgacccggc agcggatcgg cgcgccgttg gccaccggca
      181 gcgcctggtc gttgagctcg taggcgagga tcgtctgcac gtggcgcgcc tcgtcgaggt
      241 cgatgctctc gtagtagtgc gagtcgtgcc cgtcgagcgt cggcccgtcc atcgagtcga
      301 agcagcgaaa gaccacgtat ttcgcttcgg gcaacggctc gacctggtcg aggatccgac
      361 cgagctgcac gcccttccac ttgccgatga cgctccagcc ctcgacgcag tcgtggcgcg
      421 tgatctgcgt gcgcgacggc atcgctcgca gctgcgccag cgtgtacgac gccgagtgcc
      481 ggaccagccc ggtcaccatc agctcgtagt ccttgaagcc gtcgcggcgc agcgcctggt
      541 agtcggcgtc gccgggattg agcgtgccgt tgccgcgaaa ggtcggcgcg acgtcggcct
      601 gggtgaactc ctgggccatc gccgaggccg gcgcgacgat cttctgcgcc gcgtggctga
      661 gcttttgcgc gctcttgagc gtcgtcacga agccggtgtt gcccgacagc cgatcgcagc
      721 cggcgagcat ggccgagctc gccgccgcga cgaagccggc gagcgcaccg cggcgctggc
      781 gctggcgtgc ccgcatcagc gtgaaggtct tcttcatggc gacgacttct ccttcgcgtg
      841 ctctttcggc agcacgtacc agccggtgat catcgagcgg atctcgttcc agacgccggc
      901 gatgaagacc tcgacgacgt ggatgaagac gaacaggagc agcccgaacg cggcgatgaa
      961 atgcagcgac ctcgccgact gccggccgcc gagcagatcg acccacccgg tgaagacggt
     1021 gtcgatgcgc ggcgacagcg ccatgccgca cagcaccacc agcggcagca gcacgaagat
     1081 gacgacgaga taggcgaggc tttgcaggac gttgtagcga cgcgccgcct cgcccgtcgg
     1141 gtgcctgaac ttgaggtgat cgacgatcga ccggccgatg tgcctcagct cgacgcgggt
     1201 cggcaccagg tcgcgccgca ggtggccgct gaagatcgtg tagagcacgt aggcgacgcc
     1261 gttggcgacg aacagccagg cgaagaacag gtgccagtgc cgcgccatcg ccagccactg
     1321 gttgccggga atcgtcgccc agctcggaaa gccgcgcgcc gtcggcgaac cggcgacctt
     1381 cgacaggccg agcacgccgg tcgtgtcgaa ctcggcattg ccgatctgcg tcttgccgac
     1441 cggcccgtgc ggcgtgttct cggcacgcag cgacagccag ggctgaccgc tcgacgactg
     1501 cccccagtgc agcgacgggc tggcgttgaa gatgttgagg ccgctcatca gcaggatcgt
     1561 caggcacagg acgttgatcc agtgcatcac gcgaaccggc agccggtggc gatagacgcg
     1621 ccgggccggc gcggcgataa cggtggcggg caaggatgcg gcgatcgctt cggtgctcat
     1681 gctgtcgatg tctccagttc accgcctgtt tcgccgcaga cggccggcgc cggccttcac
     1741 cgacgttcaa tgatgcgccg ccatcgaccg gcctgcacgg aggtggtcac aggcgctcct
     1801 gcgacttcgt ccgcagccgg cgtcgcttct tacagcggcg gcgcgcgcag cctcatggca
     1861 gggtgatcgt gtgcaactgc ggccggtcga ggatgcgcgc ttcggcatcg acgctcgaga
     1921 tcacgccgtt ggcggcgtag cccgcgggcg actccggctc gagcgccgcg accgcgagat
     1981 tggcgagcgg ctgctccatc gtcacgtagt agccaccggc gggcatgtcg agaagcacgg
     2041 gcgacgtgcg cacgcgcagc cgcgccgcgc cgtcgctggg ccgaacgtcg cggccgatct
     2101 cgcgataggt ctcgccacgc acctcgccgg cttcatcgag ctgctgcacg tcgatgccga
     2161 gcaggcgcag ccgccgcacc gcgtcgctct cgctggccgc cagccagtag ccgcacggcc
     2221 tcggccggct cttcaggatg cgcagctcga gcgccgattg ccagctcacc gtgatgtggc
     2281 ggatcgcccc cgtcagcgga tcgagcatcg ccaggtcgtg ctcgctcgcc accggcgcgg
     2341 cctcgatcgc cacctcgccc tggcacgcac tggcgacgat gtcgcgctcg acatactggc
     2401 gcagcttgac caggtcgccg gcgtgcgacg ccgcgttgtc gagcacgttg cgcgccgacg
     2461 ccagggcgac ggcgacccgc cggcgcaggt cggtccggcc gatgccgcgg ccgcgcgtcg
     2521 ccaccatcag gctgacgatg ttcttcaggc catcggcgtt gcgcccgatc tccgggccga
     2581 cgccgcccat cgagaccgtc ttgtcggcgg catcgacggc gagcgtgtag taccactcgt
     2641 tgctgaatcc cgcggcattc aggctcgacg ccagcggcgc ccggaaccac tcctcggcgg
     2701 cgcgggcgac gaacgggcgc aggttggcga cggtggccgt atcgagcagt gcgtcgtagc
     2761 gctgcacgcc gccgaagcgg gcctcg
//
LOCUS       DASRWS010000663         7726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4281143, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000663 DASRWS010000000
VERSION     DASRWS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281143"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..287)
                     /locus_tag="VFF72_12680"
     CDS             complement(114..287)
                     /locus_tag="VFF72_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94066.1"
                     /translation="MNLALRHRFAAAIASVAITFSLLNGLALMARPAPSTTLAQAAPT
                     VVAQAATAITAVR"
     gene            605..1219
                     /locus_tag="VFF72_12685"
     CDS             605..1219
                     /locus_tag="VFF72_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HZV94067.1"
                     /translation="MASASRFSPAADRNKAPILAVLEEVLPAGAVVLEIASGTGQHAA
                     FFAAAHPSWTWQPTDADAQTMASIAARTEASTNVRPPLPLDVRLAEWPPTLGRFDAVY
                     CANMLHIAEWSTCAGLMQGASRHLVPGGVLVLYGPYLVDGEPLAPSNAAFDADLRARN
                     ASWGLRALSLVADEARQAGLALERRWIMAANNLMLALRLPPAAP"
     gene            1308..2279
                     /locus_tag="VFF72_12690"
     CDS             1308..2279
                     /locus_tag="VFF72_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94068.1"
                     /translation="MNAAVASSRLALLLAAVLVAVAAPAAARTSEACANHPVRVTAHG
                     EKTETPRDGDSGRSGACSDGHRPIVTATGIKATVRTLSRRKVVYDIAALLRAKVLSRQ
                     TLEDYGSCWPVRPGSPCACEETIDFPVLSGPEPGIAVINRALARDAQHAQRCAASDAA
                     GVLRRADSYVIYLNASPPFVSVATMNRDDDMSANGSCHGQMSFETFATSTGKRLTVAD
                     AVDGTKMAALVDAVAAGYEIYVEAGGAYPDHAAAAREARLITNRHAGQFAAGDIYVED
                     GRVFLDVEGYDLSCVAGAFHPVEIPAALLKADFARALARSTIPSRPR"
     gene            2344..3285
                     /locus_tag="VFF72_12695"
     CDS             2344..3285
                     /locus_tag="VFF72_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HZV94069.1"
                     /translation="MIIKTQDDVTAAVLAELDRATDPRFREIMTAAVRHLHAFVRESK
                     LSEMEFHQACALIARLGQLTTDSHNEVVLTAGSLGLSSLVCLLNNGENGQRETTANLM
                     GPFWRMDSPATRNGGSIVRSPTPGTPIFVNVWVRDLQGVAVEGAEVDVWHTSAEGLYE
                     NQDPAQADMNLRGKFTTDADGHVAFTSVKPAGYPIPVGGPVGALLREQGRHNLRPAHI
                     HFMIRKSGFKTQFSQVYSSDDPNLETDVQFGVTRALIGHYVLHSGEPAPDGGVGAWYS
                     LDHHFAIEPGTATLPRPPITGKAAGERAEQVVLRRRE"
     gene            3542..4801
                     /locus_tag="VFF72_12700"
     CDS             3542..4801
                     /locus_tag="VFF72_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZV94070.1"
                     /translation="MNDRRFPQISRRTFNTVAGAAGLSLGFGRMGFAADAPDSAQSII
                     GKKSGLIVHNAQLGVMETPLVELRKYAHTPKEILFNRFHFPHEGAAAWYGTTAAPSAA
                     MVDQWNLRIDGLVERPRDVSIAELHKWPQVKRVSVLQCAGNGRAYYAAKQKVAGGQWH
                     NGGMGNVEWEGVELRPFLDGMKMAVGKSATWLTAEGWDQPATPEGSDFAKSYRLDDPA
                     LDHAILALKMNGEPIPATNGGPVRLIIPGYYGNMNVKMLTYLLFAAAQSPSAYQSVGY
                     RMPKQLPSDPAVFKANDYTLENSVPTYGHAIRSAIFSPLPSDNPKPGRVEITGVAFND
                     GRSKITKVEVSTDGGRSWRESTIKPPESPWAWHHWSVMADLASGPNVLMSRAFDARGE
                     SQPIDGLTRWNPRGYEWNGADRVEIMA"
     gene            4942..5628
                     /locus_tag="VFF72_12705"
     CDS             4942..5628
                     /locus_tag="VFF72_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZV94071.1"
                     /translation="MAATCAAAELTLPDGPGRALVYGQCRTCHDLQYLIESKGVPRDT
                     WNELLDSMKQYGLRIDDHQRAQILDYLATYLGPNPPPATATAATAGQPAAGKADGASI
                     FRDQCAACHQPDGRGLTGQFPPLAGNPDLFLGRDYPARVVLAGLSGKIEVEGQGFDSV
                     MPSFGVLGDEDIAAVVNFIRGAWGNDALSHKTLAPLDGGAVATRRAKAETPQQVHAAR
                     AQLKAAAAPK"
     gene            complement(5647..6438)
                     /locus_tag="VFF72_12710"
     CDS             complement(5647..6438)
                     /locus_tag="VFF72_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZV94072.1"
                     /translation="MIVERIWTDNAYRNYNYLIACPESGEALAIDPLDHGKCLAAAKK
                     NGWRITQILNTHEHHDHTGGNAAVVAATGAKVIAHHKAGSRIAGVDRGVKAGDVIKVG
                     KTVELECLDTPGHTMCHICLRAHGDEPALFSGDTLFNAGAGNCHNGGNPEDLYATFVD
                     QLAKLPGTTRVYPGHDYIENNLRFTLAREPDNADAKALLPKVEGQDPATARVTTLDDE
                     KRINTFLRLQSASVIAALREAFPDLPEAPDAKTVFVKLRELRNAW"
     gene            complement(6549..>7726)
                     /locus_tag="VFF72_12715"
     CDS             complement(6549..>7726)
                     /locus_tag="VFF72_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HZV94073.1"
                     /translation="FSRGGDGKAAVGPMLREVLLGEAMHALGIPTTRALAVVATGQPV
                     YREVELPGAVLTRVAASHIRVGTFQYFAARRDVERVRRLAEYTIARHDADLVGVPGRF
                     LKLLERVAARQARLIAQWMNVGFIHGVMNTDNMTVSGETIDYGPCAFVEAYDPKAVFS
                     SIDSAGRYAFGNQPAIAQWNLLRLAESMLPLFADDDDEAAAQAMQVIEAFAPLYRNEW
                     LAGQRAKLGLRAIEGASDEADGALADDWLELLRVGRVDFTLAWRRLADAAAGDEAALR
                     ALFSGEASSPDGWLSRWRERCASEDGALASEGRAPITGAGAARADRMRRVNPWIIPRN
                     HRVEEALAAASDEGDLGPFDRLLAALRSPWDENAGNARYGEPAPAEVTACYRTFCGT"
BASE COUNT         1244 a         2683 c         2640 g         1159 t
ORIGIN      
        1 ttggccatgc cgtcgggcac gggaaacgag gctcccgcgt cgcccggcgc aacgaggcgc
       61 gccgatgatg gcgcgccatg gcggctgccg ttgacgcggc acggggtcgc gggtcagcgt
      121 acggcggtga tcgccgtcgc agcctgggcc acaaccgttg gtgcggcctg cgccagcgtc
      181 gtcgacggtg ccggcctggc catcagtgcg agcccgttca ggagcgagaa ggtgatcgcg
      241 acggaggcga tggcagcggc gaagcggtgg cggagggcga ggttcatagc gggttccttc
      301 gaaggtgagg aagaggcgtt gtcgatggat gtactgtcgt cccgccgacc cgcttcagtc
      361 ctttgccgaa ctgaatccgg cctgaaacgt tgtgaaagga cgccttcccg aagctccccg
      421 ccgggaaccc ggacgtgacg tctttcgacg gccgctcgtc gaatcgtcga cgctgccggc
      481 gacgagacat cgcgcgatcc cgctcgacgg cgaggatcgt gaatgcgaga gccatggacc
      541 ccgcagtcga tcgagccgaa gcgtcgcgac gcagatcgcc ttcgtgcgac gtgcagaatg
      601 gcccatggcc agcgcatcca gattcagccc cgcggccgat cgcaacaagg cgccgatcct
      661 cgccgtcctc gaagaagtgc tgcccgccgg cgccgtcgtg ctcgagatcg cgagcggcac
      721 cggccagcac gccgccttct tcgcggcggc gcacccatcg tggacgtggc agcccaccga
      781 tgccgatgcg cagacgatgg cctcgatcgc cgcgcgaacc gaggcgtcga ccaacgtccg
      841 cccgccgctg ccactcgacg tccgcttggc agaatggccg ccgacgctcg gccgcttcga
      901 cgcggtctac tgcgccaaca tgctgcacat cgccgagtgg tccacctgcg cgggcctgat
      961 gcagggcgcc tcgcgccacc tcgtgccggg cggcgtgctc gtcctctacg ggccttacct
     1021 ggtcgacggc gagccgctgg cgccgagcaa tgcggcgttc gacgccgacc tgcgggcgcg
     1081 caacgcgtcc tggggcctgc gtgcgctgtc gctcgtcgcc gacgaagcga ggcaggccgg
     1141 cctggctctc gagcgtcgct ggatcatggc ggcgaacaac ctgatgctcg cgctgcgcct
     1201 gccgccggcg gcaccctagc gaccccgcgc cgtgccccac cgcgaccggc agacacgccg
     1261 atgcgatccg ggttcatcgc aggttcgcgt cgccacgcca ccgtgagatg aacgccgccg
     1321 tcgcatcgtc gcgcctggcc ctgttgctcg ccgctgtcct ggtcgcggtc gccgcgccgg
     1381 ctgcggcgcg aacgagcgag gcctgcgcca accacccggt gcgcgtgacg gcacacggcg
     1441 aaaagaccga gacgccgcga gacggcgaca gcggtcgatc gggcgcgtgc agcgacggac
     1501 accggccgat cgtcacggca acgggcatca aggcgacggt gcgaacgctc tcgcgccgca
     1561 aggtcgtcta cgacatcgcc gccctgcttc gcgccaaggt cctctcgcgg cagaccctcg
     1621 aggactacgg atcctgctgg ccggtgcgcc cgggcagtcc atgtgcgtgc gaggagacga
     1681 tcgacttccc cgtgctgtcg gggccggagc cgggaatcgc cgtcatcaat cgcgccctcg
     1741 cgcgagacgc gcagcatgcg cagcgctgcg cggcctctga cgctgccggc gtgctgcgcc
     1801 gggccgattc ctacgtcatc tacctcaacg cgagcccgcc cttcgtgagc gtggcgacga
     1861 tgaaccgcga cgacgacatg agcgccaacg gcagttgcca cggccagatg agcttcgaga
     1921 ctttcgccac gtcgaccggc aagcggctga ccgtcgccga cgcggttgat ggtacgaaga
     1981 tggcggcgct ggtcgacgcc gtcgccgcag gctacgagat ctacgtcgag gctggcggcg
     2041 cctaccccga ccacgccgcc gcggcccgcg aggcgcgact gatcacgaac cgtcacgccg
     2101 gccaattcgc cgccggcgac atctacgtcg aggacggccg cgtgtttctc gacgtcgagg
     2161 gctacgacct cagctgcgtt gccggcgcct tccatccggt cgagatcccg gccgcgttgc
     2221 tgaaggcaga cttcgcgcgg gcgctggcac gcagcacgat tccatcgcga ccgcgatgaa
     2281 gaccggagaa gcaagccgcg cagagacggc gttgccgcag gctccccgta acccggagac
     2341 acgatgatca tcaagacgca agacgacgtg actgccgccg tcctggcgga gctcgatcgc
     2401 gccaccgatc cacgcttcag ggagatcatg accgccgcgg tgcggcacct gcacgccttc
     2461 gtccgcgagt cgaagctgag cgaaatggaa ttccaccagg cttgcgcgct gatcgccagg
     2521 ctcggacagc tcaccaccga ttcgcacaac gaggtcgtgc tgacggccgg ctcgctcggc
     2581 ctctcctcgc tggtctgcct gctcaacaac ggcgagaacg gccagcgcga gacgaccgcc
     2641 aacctgatgg gcccgttctg gcgaatggat tcgccggcca cgcgcaacgg cggctcgatc
     2701 gtgcgttcgc cgacgccggg aacaccgatc ttcgtcaacg tctgggtgcg cgacctgcag
     2761 ggcgttgcgg tcgaaggcgc ggaggtcgac gtctggcaca cctcggccga aggactctac
     2821 gagaaccagg atccggcaca ggccgacatg aacctgcgcg gcaagttcac gaccgatgcg
     2881 gatggccatg tcgccttcac gagcgtcaag ccggccggct atcccatccc cgtcggcggt
     2941 ccggtcggcg cgctcctgcg tgagcagggc cggcacaacc tgcgccccgc gcacatccat
     3001 ttcatgatcc gcaagagcgg tttcaagacg cagttctcgc aggtctattc gagcgacgac
     3061 ccgaacctcg agaccgacgt gcagttcggc gtcacccggg cgctgatcgg ccactacgtt
     3121 ctccattccg gcgagcccgc gccggacggc ggcgtcggcg cctggtactc gctcgaccat
     3181 cacttcgcga tcgagccggg aacggcgacg ctgccgaggc cgccgatcac gggcaaggcc
     3241 gcaggcgagc gcgcggagca agtggtgctg cgacgccgcg agtgaatcgg ccacgcgtcg
     3301 acctcgaacc tcgcgtcggc gcgcgctgcc gtgcgtggca ttcatcactc aaatgtgccg
     3361 gggttttccc gggatcgaag atgtttgcag ggcgaatgcg gcgctgccga cgatatgctc
     3421 gacgttccgc gcatccggac cctgatcacg aactcgttct cgcagccctc atcgcggctc
     3481 catcggtgag agggcggctc cagacccggc tggaggcaga ggaccgttga ggaaggaccc
     3541 catgaacgat cgacgctttc cgcagatttc cagaagaacc ttcaacaccg tggccggtgc
     3601 ggcgggactg tccctcggct tcggccgcat gggatttgcc gccgacgcac ccgactccgc
     3661 gcaaagcatc atcggcaaga agtccggcct gatcgtgcac aacgcccagc tcggcgtgat
     3721 ggagacgccg ctggtcgagc tgcgcaagta cgcccacacg cccaaggaga tcctgttcaa
     3781 ccgcttccac tttccgcacg aaggcgccgc tgcctggtac ggaacgacgg cagcgccctc
     3841 ggcggcgatg gtcgatcagt ggaacctgcg catcgatggc ttggtcgagc ggccgcgcga
     3901 cgtgagcatc gccgaactgc acaagtggcc gcaggtcaag cgcgtctcgg tgctgcagtg
     3961 cgcgggcaac ggacgggcct actacgccgc caagcagaag gtggctggcg gccagtggca
     4021 caacggcggc atgggcaacg tcgaatggga aggcgtcgag ctgaggccgt tcctcgatgg
     4081 catgaagatg gccgtcggca aatcggcgac gtggctgacc gccgagggct gggaccagcc
     4141 cgccactccg gaaggttccg atttcgccaa gagctatcgc ctggacgatc ccgcgctcga
     4201 ccacgcgatc ctcgccctca agatgaacgg cgaaccgatc ccggcaacca acggcggccc
     4261 ggtgcgcctg atcatcccgg gctactacgg caacatgaac gtcaagatgc tgacgtacct
     4321 gttgttcgcg gccgcgcaaa gcccgagcgc ctaccagtcg gtcggctacc ggatgcccaa
     4381 gcagctgccg tccgatccgg cggtgttcaa ggccaacgac tacacgctcg agaacagcgt
     4441 gcccacctat ggccacgcga tcaggagcgc gatcttctcg ccgctgcctt cggacaatcc
     4501 gaagcccggc cgggtcgaga tcaccggcgt cgccttcaac gacggcaggt cgaagatcac
     4561 caaggtcgag gtctcgaccg acggcggcag gtcgtggcgc gaatcgacga tcaagccgcc
     4621 cgagagtccc tgggcctggc accactggtc ggtgatggcc gacctcgcca gcgggcccaa
     4681 cgtgctgatg tcgcgggcgt tcgatgcccg gggggagagc cagccgatcg acggcctgac
     4741 gcgatggaac ccgcgcggct acgagtggaa cggcgccgat cgcgtcgaga tcatggcgtg
     4801 aggagggtcg cggtccgcgc caccgcggtg gcgcccgcgg ccttcgaaag ttcatcgacc
     4861 cccacagcct ccccgaagag gcgtcgatac ggcagcgcga gggccgcgcg cgcactgtcg
     4921 ctggcgcttt gcgcgtggtg gatggccgcg acctgcgccg cggccgagct gacgttgcct
     4981 gacggaccgg ggcgagcgct ggtttacggg cagtgtcgca cctgccacga cctgcagtac
     5041 ctgatcgagt ccaagggcgt gccccgcgac acctggaacg agctgctcga cagcatgaag
     5101 caatacggcc tgcgcatcga cgaccatcaa cgggcgcaga tcctcgacta cctcgcgacc
     5161 tatctcggcc cgaaccctcc gcccgcgacc gcgaccgccg cgacggccgg gcagcctgcg
     5221 gccggcaaag ccgatggcgc gtcgatcttt cgcgatcaat gcgccgcttg ccatcagccc
     5281 gatggccgcg ggctcaccgg gcaatttccg cccctggcgg gcaatcccga cctgttcctg
     5341 ggtcgcgact acccggcccg cgtcgtgctc gcgggcctga gcggaaagat cgaggtcgag
     5401 ggacaaggct tcgacagcgt gatgccgtcg ttcggcgtcc tcggcgacga agacatcgcc
     5461 gcggtggtga atttcatccg tggggcctgg ggcaacgatg cgctgagcca caagaccctc
     5521 gcgccgctcg acggcggcgc ggtcgccacc cggcgcgcca aggccgagac gccgcagcag
     5581 gtccacgcgg cacgtgcgca actgaaggct gccgcggcgc cgaagtgagg ccgccggcgc
     5641 ggcgaatcac caggcgttgc gcagctcgcg aagcttgacg aacacggtct tcgcatcggg
     5701 cgcttcgggc aggtcgggaa acgcctcgcg caaggcggcg atgacgctgg cgctttgcag
     5761 tcgcaggaag gtgttgatcc gcttttcgtc gtcgagcgtc gtcaccctgg ccgtcgccgg
     5821 atcctgaccc tccaccttcg gcaacagcgc tttcgcatcg gcgttgtcgg gctcgcgggc
     5881 gagcgtgaag cgaaggttgt tctcgatgta gtcgtgtccc gggtagacgc gcgtcgtgcc
     5941 cggcagcttc gcgagctgat cgacgaaggt ggcgtagagg tcttccggat tgcccccgtt
     6001 gtgacaattg ccggcgccgg cgttgaagag ggtgtcgccc gagaagaggg ccggctcgtc
     6061 gccgtgcgcg cgcaggcaga tgtggcacat cgtgtggccc ggcgtgtcga ggcattcgag
     6121 ctcgacggtc ttgcccacct tgatcacatc cccggccttg acgccgcgat cgacgccggc
     6181 gatgcgcgag ccggccttgt gatgcgcgat caccttggct cccgtcgccg cgacgaccgc
     6241 agcgttgcca cccgtgtgat cgtggtgctc gtgggtgttg aggatctgcg tgatccgcca
     6301 gccgttcttc ttcgcggcgg ccaggcattt gccgtgatcg agcgggtcga tggccagcgc
     6361 ctcgccgctt tcggggcagg cgatcaggta gttgtagttt cggtaggcgt tgtcggtcca
     6421 gatgcgctcg acgatcatgg gcgtgctcca cgggttcgat ggttcgggcc atcatgccac
     6481 cgcaccgccg ctgccacgcg cgcacgacac gaccgccatc ggccgcgaag ggatgacccc
     6541 ctcgaagctc aggtgccgca gaacgttcga tagcaggccg tgacctcggc cggcgccggc
     6601 tcgccatagc gggcgttgcc ggcgttctcg tcccagggac tgcgcaaggc ggcgagaagt
     6661 cggtcgaacg gaccgagatc gccctcgtcc gaggcggcgg cgagcgcctc ttcgacacga
     6721 tggttgcgcg ggatgatcca cgggttgacg cggcgcatgc ggtccgcccg cgcggcaccg
     6781 gcacccgtga tcggagcccg gccttcgctg gcgagggctc cgtcctcgct ggcgcagcgc
     6841 tcgcgccagc gcgaaagcca cccgtcgggc gaactcgctt cgccggaaaa gagcgcgcgc
     6901 agcgccgctt cgtcgcccgc cgcggcatcg gcgagacgcc gccaggcgag ggtgaagtcg
     6961 acgcgcccga cccgcagcaa ctcgagccag tcgtcggcga gcgcgccgtc cgcctcatcg
     7021 ctggcgccct cgatcgcgcg caggccgagc ttggcgcgct ggccggcgag ccactcgttt
     7081 cggtagagcg gcgcgaacgc ctcgatcacc tgcatcgcct gcgcggcggc ctcgtcgtcg
     7141 tcgtcggcga agagcggcag catcgattcc gccaggcgca gcaggttcca ctgggcgatc
     7201 gcgggctggt tgccgaacgc gtagcgacct gcgctgtcga tcgagctgaa caccgccttc
     7261 gggtcgtacg cctcgacgaa ggcgcacggc ccgtagtcga tggtctcgcc cgacacggtc
     7321 atgttgtcgg tgttcatgac gccgtggatg aagccgacgt tcatccattg cgcgatcaat
     7381 ctcgcctggc gcgcggcgac gcgctcgagc agcttcagga agcgcccggg cacgccgacc
     7441 agatcggcgt cgtgccgggc gatcgtgtac tcggcaaggc gtcgcacgcg ctcgacatcg
     7501 cgacgggcgg cgaagtactg gaaggtgccg acgcggatgt ggctggcggc gacgcgcgtg
     7561 agcaccgcgc ccggaagctc cacttcccgg tacacgggct gtccggtggc gaccacggcg
     7621 agcgcgcgcg tcgtcggaat gccgagcgcg tgcatcgctt cgccgagcag cacctcgcga
     7681 agcatcggtc cgaccgcggc cttgccgtcg ccgccgcgcg agaacg
//
LOCUS       DASRWS010000664         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1411437, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000664 DASRWS010000000
VERSION     DASRWS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411437"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..112
                     /locus_tag="VFF72_12720"
     CDS             14..112
                     /locus_tag="VFF72_12720"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HZV94074.1"
                     /translation="MVLRIHWQALRLWSRRVPFLSKPRPPQSFVSH"
     gene            143..1363
                     /locus_tag="VFF72_12725"
     CDS             143..1363
                     /locus_tag="VFF72_12725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZV94075.1"
                     /translation="MTTLSRRSVPDRAPSAPAAARAVLALLGNLRHGTLEVRLPDGSM
                     LHFGGDPASGPRAAISLRNWNVCAAAMKLGDIGFAESFIAGDWTSPDLPSLLEVFTTN
                     RDEVEAMLYGTWWGSLLYRARHLLRRNSRRGSKKNIHAHYDLGNDFYRQWLDPTMSYS
                     SAWFDGDRSGDLVEAQRAKSRRALAQCGVGAGDRVLEIGCGWGALAELAGREFGAEVT
                     GVTLSGAQLDWASRRVAAAGVAADLRLQDYRDIDDGPYDAIVSIEMFEAVGREYWRDF
                     FDVVRSRLRPGGRACVQTITIRDDLFDRYVRSTDFIQQYIFPGGLLPSRREFRAAAGS
                     AGLRIEGELEFGLDYATTLRHWRERFLAEEGAVRRLGFDTRFIRIWEFYLAYCEAAFA
                     SRNTSVVQFTLQHA"
     gene            1536..1877
                     /gene="raiA"
                     /locus_tag="VFF72_12730"
     CDS             1536..1877
                     /gene="raiA"
                     /locus_tag="VFF72_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZV94076.1"
                     /translation="MNLTISGHHLEVTPALREYVLTKLDRVMRHFDQVVDVTVLLCVE
                     KLSEKERRQRAEVTLHVKGKDIFAECAAEDLYAAIDQLMDKLDRQVVRHKGKVQDHHH
                     ASPKRTDATLN"
     gene            2027..2497
                     /locus_tag="VFF72_12735"
     CDS             2027..2497
                     /locus_tag="VFF72_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZV94077.1"
                     /translation="MNRLAAILPVADVLIDVDATSKKRVFEHAGLLFENQHSIARATV
                     TDNLFARERLGSTGLGHGVAIPHGRIKGLKNPLAAVLRLRDAIAFDAPDDEPVALLIF
                     LLVPEAATQRHLEILSEIAEMLSDRELRERLKNESDAAGLHRLISAWEPLRSVA"
     gene            2558..3508
                     /gene="hprK"
                     /locus_tag="VFF72_12740"
     CDS             2558..3508
                     /gene="hprK"
                     /locus_tag="VFF72_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZV94078.1"
                     /translation="MKPIVISAEALFEEFQSILHWEWIAGHAHPERHFDDAAIRDAGS
                     AADLVGYLNYIHPNRVQIVGRREVAYLNDGSAEVIERRLTRIITLDPPVLIVADAQVP
                     PDRLVALCDRAEIPLFVTSDSAGHVIDLVRGYLGQHFAERTTRHGVFMDILGLGVLLT
                     GESGLGKSELGLELISRGHGLVADDAVDLYRVSQTALEGRCPDLLLNLLEVRGIGLLD
                     IKAIFGETAVRRKMRLKLIVHLVRKETMDREFERLPYEPLNEEILEVPVRKTVITVDA
                     GRNLAVLVEAAVRNTILQLRGIDTYTEFIERHHKAMSKPD"
     gene            complement(3519..3965)
                     /gene="fur"
                     /locus_tag="VFF72_12745"
     CDS             complement(3519..3965)
                     /gene="fur"
                     /locus_tag="VFF72_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZV94079.1"
                     /translation="MNHADELKNSGLKATLPRIKVLEVFQKTKERHMSAEDVFRVLLA
                     EHADVGLATVYRVLMQFEQAGILSRNHFETGKAVFELNEGTHHDHLVCLDCGRVEEFY
                     DAEIEKRQRAVAESRGFELQEHALALYATCTKKNCQFKVKGGHSDR"
BASE COUNT          664 a         1467 c         1360 g          734 t
ORIGIN      
        1 gctgacgatc gccatcgtcc tcaggatcca ctggcaggcg ctgcgtctgt ggtccaggcg
       61 cgtgccgttc ctgagcaagc ccaggccgcc gcagtctttc gtcagccact gatccgaccc
      121 cttccatcgc cgcccgccgc ccatgacgac actttctcga cgatcggttc ctgatcgcgc
      181 gccgtccgct ccggcggccg cacgcgccgt gttggcgctg ctcggcaatc ttcgccacgg
      241 cacgctggag gtgcggctgc ccgacggctc gatgcttcac ttcggcggcg atccggcgag
      301 cggaccccgg gcggcgatct cgcttcgcaa ctggaacgtt tgtgccgcgg cgatgaagct
      361 cggcgacatc ggctttgccg agagcttcat cgccggcgac tggacgagcc ccgaccttcc
      421 cagcctgctc gaggtcttca ccaccaatcg cgacgaggtc gaggcgatgc tctacggcac
      481 gtggtggggc tcgctcctct accgcgcgcg tcacctgttg cggcgcaact cgcgacgcgg
      541 ctcgaagaag aacatccacg cccattacga cctcggcaac gacttctacc ggcagtggct
      601 cgacccgacg atgagctaca gcagcgcctg gttcgacggc gaccgatcgg gggacctcgt
      661 cgaggcgcaa cgtgcgaagt cgcgcagggc tctcgcgcag tgcggcgtcg gcgccggcga
      721 ccgcgtcctc gagatcggct gcggctgggg ggcgctggcc gagctcgccg gccgcgaatt
      781 cggcgcggag gtgaccgggg tgacgctgtc cggcgcgcag ctcgactggg cgagcaggcg
      841 cgtcgccgcg gccggcgtcg ccgccgacct gcgcttgcag gactatcgcg acatcgacga
      901 cggcccctac gatgcgatcg tctcgatcga gatgttcgag gcggtcggtc gcgagtactg
      961 gcgcgatttc ttcgacgtgg tgcggtcgcg attgcgtccc gggggcaggg cgtgcgtcca
     1021 gaccatcacc atccgcgacg atctcttcga ccgctatgta cgctccaccg atttcatcca
     1081 gcagtacatc ttcccgggcg gcctgctgcc gagccggcgc gagttccgcg ccgctgccgg
     1141 atcggccggc ctgcggatcg aaggcgagct cgagttcggc ctcgactacg cgaccacgct
     1201 gcgtcactgg cgcgaacgct tcctggccga agaaggtgcg gttcgccggc tgggcttcga
     1261 cacccggttc attcgcatct gggagttcta tctcgcctac tgcgaagccg cgttcgccag
     1321 tcgcaacacg agcgtcgtcc agttcacgct ccagcacgcc tgagactcgg gcagtgacgc
     1381 gggtcgcccg gggaacggcg gggcggtcga gcggtcgtcc ccgccgccgg tcgaggcgat
     1441 agactggctc ggcccggccg gcaacagccc tcctcgctgc gctggcttcg ggcgctctgc
     1501 tactgtcctc tgccgccctc ccgaaaggat ccttcatgaa tctgacgatc agcggtcatc
     1561 acctcgaggt cacacccgca ctgagggaat acgtgctcac gaagctggac cgcgtgatgc
     1621 ggcatttcga tcaggtcgtc gacgtcaccg tgcttctgtg cgtcgagaag ctcagcgaaa
     1681 aggagaggcg ccagcgcgcc gaagtgaccc tgcacgtcaa gggcaaggac atcttcgccg
     1741 agtgtgcggc cgaagacctc tacgccgcga tcgaccagct gatggacaag ctcgatcgcc
     1801 aggtggtccg gcacaagggc aaggtccagg atcaccacca cgcgtcgccc aagcgcaccg
     1861 acgcgacgct caactgaatc ctcgcgccgc ggcactgttg ttgcggcgca gcaatcccgg
     1921 cccggccatc cgggggaatc ggtcctgcgt gggcgccggc aacgccgtgg aaccataatt
     1981 catcgccttg tcacttcacc cggcgctcgc tgcgggggtc tcgtcaatga atcgtctcgc
     2041 cgccatcctg ccggtcgccg acgtcctgat cgatgtcgat gccacgagca agaagcgggt
     2101 cttcgagcat gccggcctgc tgttcgagaa ccagcattcg atcgctcgcg ccaccgtgac
     2161 cgacaacctg ttcgctcgcg aacggctggg ctccacgggg ctcggacacg gcgtcgcgat
     2221 tccacatggc cgcatcaagg ggctgaagaa tccgctggcc gcggtgctgc gcctgcgtga
     2281 cgcgatcgcg ttcgatgcgc ccgacgacga gcccgtcgcg ctgctcatct tcctgctcgt
     2341 tcccgaagcg gcgacgcagc gtcacctgga gattctttcc gagatcgccg agatgctgtc
     2401 ggatcgcgag ctgcgcgagc gcctgaagaa tgagagcgac gccgccggcc tgcatcgatt
     2461 gatctcggcg tgggaaccgc tgcgatcggt cgcttgaagc ggccggggtc gtgtcgatga
     2521 ccggcacgcc ggccatgccc actgccacca ggcgccgatg aagccgatcg tcatcagcgc
     2581 cgaggcgctg ttcgaggaat tccagtcgat cctgcactgg gagtggatcg ccggccatgc
     2641 ccaccccgag cggcacttcg acgacgccgc gatccgcgac gcgggctcgg ccgccgactt
     2701 ggtgggctac ctcaactaca tccacccgaa ccgcgtgcag atcgtcggcc ggcgcgaggt
     2761 cgcctatctc aacgacggct ccgccgaagt gatcgagcgc cgtctcacac gcatcatcac
     2821 gctcgaccct ccggtgctca tcgtcgccga cgcacaggtg ccacccgatc gactcgtcgc
     2881 gctgtgcgat cgggccgaga tcccgctgtt cgtcacctcg gattccgccg gccatgtgat
     2941 cgacctcgtg cgcggctacc tcggccagca tttcgccgaa cggacgacgc gccacggcgt
     3001 gttcatggac attctcggcc tgggcgtgct gctgaccggc gagtcgggtc tcggcaagag
     3061 cgaactcggc ctggagctca tctcgcgcgg ccacggtctg gtggccgacg acgcggtcga
     3121 tctctaccgc gtctcgcaga ccgcgctcga gggccgatgc ccggacctgc tgctcaacct
     3181 gctcgaggtg cgcggcatcg gcctgctcga catcaaggcg atcttcggcg agaccgcggt
     3241 gcgccgcaag atgcgcctga agctgatcgt ccatctggtt cgcaaggaga cgatggatcg
     3301 cgaattcgag cggctgccgt acgagccgct caacgaggaa atcctcgagg tcccggtgcg
     3361 aaagaccgtg atcacggtcg atgccggccg aaacctcgcc gtgctcgtcg aggcggcggt
     3421 gcgcaacacg atcctgcagc tgcgcggcat cgacacgtat accgagttca tcgagcgtca
     3481 ccacaaggcg atgagcaagc ccgactagcg gcgcgtggtc agcggtcgga gtgaccgccc
     3541 ttcaccttga actggcagtt cttcttcgtg cacgtggcgt agagggcgag ggcgtgctcc
     3601 tgcaactcga agccccggct ctcggcgacg gcgcgctggc gcttttcgat ctcggcgtcg
     3661 tagaactcct cgacgcggcc gcaatcgagg cacaccaggt ggtcgtgatg cgtgccctcg
     3721 ttcagctcga agacggcctt gccggtctcg aaatggttgc ggctgaggat tccggcctgc
     3781 tcgaactgca tcaggacgcg gtagacagtc gccaggccga cgtcggcgtg ctccgcgagc
     3841 aggacacgaa agacgtcctc ggcgctcatg tgccgttcct tggtcttctg gaagacctcg
     3901 agcaccttga tgcgcggcag cgtcgccttc aggccgctgt tcttcagttc gtcggcgtgg
     3961 ttcatggcaa tccgggcgtc ggtgcggggc gccgatagaa tcgcttcatc atacgagctt
     4021 gggcgggcgc tgcggcaccg gtcccttccc attccatgcc cacgaccccg ctgcgcacat
     4081 atcgcgcgct gtcccgagcg gccctgtcgc tgcctctcgc cgtcacgctg gccggctgcc
     4141 attcgcttca gaccaacgac aacttcctgg gcgtcatcac gccctaccgt ctcgagatcg
     4201 tccagggcaa cgtcatcacc cgcga
//
LOCUS       DASRWS010000665         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4282986, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000665 DASRWS010000000
VERSION     DASRWS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4282986"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1999)
                     /locus_tag="VFF72_12750"
     CDS             complement(<1..1999)
                     /locus_tag="VFF72_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZV94080.1"
                     /translation="MQATPAQAASATVASLRPPVRPAPAASAYQGYQTLRRNGAVVAF
                     EPNKIAVALMKAFLAVHGTQGAASASVRETVDGLTESVVRGLLRSRPGGGTFHIEDVQ
                     DQVELGLMRGGHHEVARAYVLYRERRAQERAKQAPAPRAAEPSLMVTDRGNRVPLDSA
                     RLQTLIESACANLGDDVRPDPILAETKRNLYDGVPIDEVHKAAILAARTLIEKDPGYS
                     RATARLLLHTIRREILGEELLHSDMHARYAEYFPQFVKKGVQAELLDERLLQYDMARL
                     GAALKADRDLQFDYLGLQTLYDRYFLHIEDSRIELPQAFFMRVAMGLALGEIDREARA
                     IEFYEVLSTFDFMSSTPTLFNAGTRRSQLSSCYLTTVADDLDGIYEALKENALLSKFA
                     GGLGNDWTNVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETW
                     HLDIEEFLELRKNTGDDRRRTHDMNTSNWVPDLFMKRVMEGGEWTLFSPSTCPDLHDK
                     FGREFESAYTRYEEKAARGEIKLFKKMKAADLWRRMLTMLFETGHPWITFKDACNVRS
                     PQQHVGVVHSSNLCTEITLNTSESEIAVCNLGSVNLAQHVADGVLDQVKLKKTVATAM
                     RMLDNVIDINYYAVKKARDSNLRHRPVGLGIMGFQDALYEVG"
     gene            complement(2343..2939)
                     /gene="ampD"
                     /locus_tag="VFF72_12755"
     CDS             complement(2343..2939)
                     /gene="ampD"
                     /locus_tag="VFF72_12755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HZV94081.1"
                     /translation="MSDRGGRSDPAEAGWQSSGWLDQARHVPSPNFDTRPARTPVDLV
                     VIHSISLPPGEYGGDAIERLFTNRLDAAAHPYFETIRGLRVSSHFLILRDGALCQFVG
                     CDARAWHAGRSVWRGRADCNDYSVGVELEGLEGGLFEAAQYTRLAALLRRVAGRYPVR
                     SVAGHEHVAPGRKHDPGAAFDWPLLRRLTDGAPFEFAA"
     gene            complement(2936..>3419)
                     /locus_tag="VFF72_12760"
     CDS             complement(2936..>3419)
                     /locus_tag="VFF72_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZV94082.1"
                     /translation="ERLARDAGVWPPPRLTRDALVHLARYPFPGNVRELENLLHRAVA
                     LSGGEELDVYDLGLPESVFTDSAAQELDPAHGRDSAAGESARAAASLLPVEEPLPSDL
                     AGYLDNVERDILVRALEHHRFNRTAAGISLGLSLRQMRYRMARLNVNVGGDPGSADRE
                     "
BASE COUNT          556 a         1118 c         1161 g          584 t
ORIGIN      
        1 cgccgacttc gtacagcgcg tcctgaaagc ccatgatccc caggccgacc ggacgatggc
       61 ggagatttga atcgcgtgcc ttcttcaccg cgtagtaatt gatgtcgatg acgttgtcga
      121 gcatgcgcat cgccgtggcg accgtcttct tcagcttgac ctggtcgagc accccgtcgg
      181 cgacatgctg ggcgaggttg accgagccga gattgcagac ggcgatctcg ctctcgctcg
      241 tattgagcgt gatctcggtg cagaggttgc tcgaatggac caccccgacg tgctgctggg
      301 gcgacctcac gttgcacgcg tccttgaagg tgatccaggg gtggcctgtc tcgaacagca
      361 tcgtcagcat ccgccgccag aggtcggccg ccttcatctt cttgaagagc ttgatctcgc
      421 cgcgggccgc cttttcctcg tatcgcgtgt aggcactttc gaactcgcgc ccgaacttgt
      481 catgcaggtc ggggcaggtc gacggcgaga acagcgtcca ctcgccgccc tccatgacac
      541 gcttcatgaa gaggtcgggg acccagttcg acgtgttcat gtcgtgcgtg cgccgccggt
      601 cgtcgccggt gttcttgcgc agctcgagga actcctcgat gtcgaggtgc caggtctcga
      661 ggtaggcgca gacggcaccc ttcctcttgc cgccctggtt gacggcgacc gcggtgtcgt
      721 tgacgacctt caggaacggc acgacgccct ggctcttgcc attggtgccc ttgatgtggc
      781 tgccgagcgc ccggacgttc gtccagtcgt tgccgaggcc gccggcgaac ttggagagaa
      841 gcgcgttttc cttcagcgcc tcgtagatgc cgtcgaggtc gtcggcgacc gtcgtgaggt
      901 agcagctcga cagctgcgag cggcgcgtcc cggcgttgaa gagcgtcggt gtcgagctca
      961 tgaagtcgaa tgtcgacagg acctcgtaga actcgatggc ccgggcctcg cgatcgatct
     1021 cgcccagcgc cagccccatc gccacccgca tgaagaaggc ctgcggaagc tcgatgcggc
     1081 tgtcctcgat gtgcaggaaa tagcggtcgt agagcgtctg cagcccaagg tagtcgaact
     1141 gcaggtcgcg atcggccttc agcgcggcgc cgagcctggc catgtcgtac tggaggagcc
     1201 gctcgtcgag caattcggcc tgcacgcctt tcttcacgaa ctgcgggaaa tactccgcgt
     1261 agcgggcgtg catgtcggaa tgcaggagtt cctcgccgag gatctcgcgg cggatcgtgt
     1321 gcaacagcag ccgcgccgtc gcccgcgaat agccgggatc cttctcgatc agggtacggg
     1381 cggcgaggat cgccgccttg tgcacctcgt cgatcggcac accgtcgtag aggttgcgct
     1441 tggtctcggc caggatcgga tcggggcgta catcgtcgcc aaggttggcg caggccgact
     1501 cgatcagcgt ctgcaatcgg gccgagtcga gcggcacgcg gttgccgcgg tcggtgacca
     1561 tgagcgacgg ctcggccgca cgaggcgccg gagcctgctt tgcccgctcc tgggcgcgac
     1621 gctcgcgata gagcacgtag gcacgtgcga cttcgtgatg gccgccgcgc atcaggccga
     1681 gctcgacctg gtcctgcacg tcctcgatgt ggaaggttcc gccccccgga cgcgagcgca
     1741 gcaggccgcg aacgaccgac tcggtcaggc cgtcgaccgt ctcacggacg ctggccgagg
     1801 ccgccccctg ggtaccgtgg acggccagga atgccttcat caaggcgacg gcgatcttgt
     1861 tcggctcgaa cgcgaccacg gcgccattgc gccgcaaggt ctggtagccc tgataggccg
     1921 aggcggctgg cgctggccgg accggcggcc gaagcgaggc gacggtcgct gatgccgcct
     1981 gagccggggt tgcttgcatg aaatctcctc tcgtgtccgc gagccggcgg gtcgccgtcg
     2041 aagacgttgt cgtcatcgtc ccggctcgcg ctccaaattc gggcacgcaa ccggaaaggg
     2101 gtgaaaaagt tcatcggaaa tcggctcttc cggcccgtcc cgagcggttc agcatagcac
     2161 cgccggacac tatatctggg gtcgatgttg ccaacaagca ctacctatag cgtattcacc
     2221 tgtgaaattt tcacgcaagg gaagtggtca ttcccgacgt ggcaggcatc ccgtgagagg
     2281 aaccgccctc gaagaaggcg aactcgcgcg tgcctcggga gccaagcccg gcagcggccg
     2341 gctcaggcgg cgaactcgaa cggcgcgccg tcggtcaagc gacgcaggag cggccagtcg
     2401 aacgcagctc ccggatcgtg ctttcgtccg ggcgcgacgt gctcgtggcc ggccaccgag
     2461 cggaccggat agcggccggc gacgcgtcga agcagcgccg cgagcctcgt gtactgcgcc
     2521 gcttcgaaaa gaccgccttc gagtccctcg agctcgacgc cgaccgagta gtcgttgcaa
     2581 tccgcgcgcc cgcgccagac cgaccggcca gcgtgccagg cccgcgcgtc gcagccgacg
     2641 aactggcaca gggcgccgtc gcggagaatc aggaaatgcg acgacacgcg caaaccgcga
     2701 atcgtctcga agtagggatg cgctgcggca tcgagtcgat tcgtgaacag tcgctcgatg
     2761 gcatcgccac cgtattcgcc tggcggcagg ctgatggaat ggatgacgac gagatcgacc
     2821 ggcgtccgcg cgggccgagt gtcgaagttg ggtgaaggca cgtgcctcgc ctggtcgagc
     2881 cagccgctcg actgccaacc ggcttccgcc gggtcgcttc gaccccctcg atcgctcact
     2941 cgcggtcggc gcttcccgga tcgccgccca cgttgacatt gagccgcgcc atccggtagc
     3001 gcatctggcg cagcgaaagg ccgaggctga ttccggcggc cgttcggttg aaacgatggt
     3061 gctcgagcgc tcgcaccagg atgtcgcgct cgacgttgtc gaggtatccg gccagatcgc
     3121 tggggagcgg ctcctcgacg ggcagcaggc tcgccgcggc ccgggcactt tcgcctgcgg
     3181 cgctgtcccg accgtgggcc gggtcgagct cctgggccgc gctgtcggtg aagaccgact
     3241 ccggaagacc gaggtcgtag acgtcgagct cctcgccgcc ggacagggcg acggcgcggt
     3301 gcagcaggtt ctcgagctcg cgcacgttgc ccgggaacgg gtagcgcgcg aggtggacga
     3361 gcgcgtcacg cgtcagccgc ggcggcggcc agacgccggc gtcacgagcc agccgctcg
//
LOCUS       DASRWS010000666         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3719375, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000666 DASRWS010000000
VERSION     DASRWS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719375"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1466
                     /gene="thrC"
                     /locus_tag="VFF72_12765"
     CDS             63..1466
                     /gene="thrC"
                     /locus_tag="VFF72_12765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZV94083.1"
                     /translation="MRYLSTRGEAAGRRFSDILLEGLAPDGGLYLPERYPRVDRNLLS
                     RWRHLPYAELAFEILSLYIDDIPASDLQALARRTYRADIFGDARIVPLRRLEPGLFVL
                     GLSNGPTLAFKDMAMQFLGHAFEYELARRGRHLNVLGATSGDTGSAAEQALRGKRGVQ
                     VFMLSPLGRMSPFQQAQMYSLQDANIHNIAIDGVFDDCQDLVKGVSADLDFKQHHAIG
                     TVNSINWARLLAQVVYYFAGYFQATADDARDVDFAVPTGNFGNICAGHIARQMGLPIR
                     RLVLATNENDVLDEFFRTGTYRVRTAAETVETSSPSMDISKASNFERFVFDLVERDPA
                     RTRELFAQVESSRSFSLSPAEQGRLAAFAMTSGRSTSADRLATIAGVHERFGEVIDPH
                     TADALKVAREHAEADTPMIVLETALPAKFSETVREAIGGEPPRPARFDGIESLPRRFE
                     TMPPSLSRLKAFIAEND"
     gene            1470..2720
                     /gene="glp"
                     /locus_tag="VFF72_12770"
     CDS             1470..2720
                     /gene="glp"
                     /locus_tag="VFF72_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZV94084.1"
                     /translation="MSTASPAPATPPLLSLDDAVAQMLARVQIPAADRAEEVSAFDAL
                     HRVLAVDVRSHVDVPPADNSGMDGYALRSADVPVAGTVLPVSQRIPAGVVGSALEPGT
                     AARIFTGAPIPAGADAVVMQEKCGVVDGAVRIDVVPAAGQSVRRRGEDVRRGSTVLVA
                     GTRLTPQALGMAASVGVGKLRVGRRPRVALFSTGDELAMPGDVLEPGAIYNSNRFTLR
                     GLIEALGGACEDFGIVPDRLDATRETLRRAAEGNDLVVTCGGMSVGEEDHMKPAVEKE
                     GRLDLWQIAMKPGKPLAFGEVFRRDGSSAWFVGLPGNPVSSLVTFLLAVRPVILKLQG
                     ASDLAPRPIAMRADFSWPRPDRRREFLRVRRDATGGLDLFAHQGSAVLTSTVWADGLV
                     DNPAGAAIAPGDFVRYLAFAELLA"
     gene            2717..2968
                     /locus_tag="VFF72_12775"
     CDS             2717..2968
                     /locus_tag="VFF72_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZV94085.1"
                     /translation="MKIEIRYFASIREALGPAESVEVAAGSDVAALRDSLVARGGSHA
                     EALGRARLLRSARNRVLCAESALLDDGDEVGFFPPVTGG"
     gene            2970..3470
                     /locus_tag="VFF72_12780"
     CDS             2970..3470
                     /locus_tag="VFF72_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZV94086.1"
                     /translation="MSRHVRIQVEDFDIGAEVAALRAGDGGVGAVAVFIGTVRDRAGA
                     EAIASLELEHYPGMTEAAIESMIDASFARFDLRGARVVHRVGQLAPQAQIVLVAVASA
                     HRGEAFQACEFLMDYLKTQAPFWKKESSPSGARWVDARIADDAALARWGIVSGNALAP
                     PEASPP"
BASE COUNT          494 a         1243 c         1193 g          580 t
ORIGIN      
        1 gcgatgcagg cgttgccgac ggtgctggcg acgatcgtgc gcatccgcaa ggaagatctc
       61 ggttgaggta cctcagcacg cgcggcgaag cggcagggcg ccgcttcagc gacattcttc
      121 tcgaaggact cgcgcccgac ggtggcctct acctgccgga acggtatccg cgcgtcgacc
      181 ggaacctcct gtcacgctgg cgccacttgc cgtacgccga gctcgccttc gagatcctgt
      241 cgctctacat cgacgacatc ccggcgtccg atctgcaggc attggcgcga cgcacctatc
      301 gcgccgacat cttcggcgac gcccggatcg ttccgctgcg ccggctcgag ccgggcctct
      361 tcgttctggg cctgtcgaac ggcccgacgc tcgccttcaa ggacatggcg atgcagttcc
      421 tgggccacgc gttcgaatac gagctggcgc ggcgcgggcg ccacttgaac gtgctcggcg
      481 cgacctcggg cgacaccggc agcgctgccg agcaggcgct gcgcggcaag cgcggcgtcc
      541 aggtcttcat gctctcgccg ctgggccgga tgagcccgtt ccagcaggca cagatgtaca
      601 gcctgcagga cgccaacatc cacaacatcg ccatcgacgg cgtgttcgac gattgccagg
      661 atctcgtcaa gggcgtctcc gcggatctcg acttcaagca acaccacgcg atcggcaccg
      721 tcaattcgat caactgggct cgccttctcg cacaggtggt gtactacttc gcaggctact
      781 tccaggcgac ggcggacgat gcgcgcgacg tcgatttcgc ggtgccgacg ggaaacttcg
      841 gcaacatctg tgccggtcac atcgcccgcc agatgggctt gccgatccgc cgcctcgtcc
      901 tggccaccaa cgagaacgac gtgctcgacg agttcttccg caccggcacc taccgtgtcc
      961 gcacggccgc cgaaacggtg gagacgtcga gtccgtcgat ggacatctcg aaggcctcga
     1021 acttcgagcg cttcgtgttc gacctggtcg agcgcgaccc ggcgcgcacc cgcgagctgt
     1081 tcgcgcaggt ggagtcgagt cgttccttct cgctgagccc tgccgagcaa gggcgtctcg
     1141 ccgcctttgc gatgaccagc ggccgcagca cgagcgcaga tcgcctcgcg accatcgccg
     1201 gcgttcacga acgtttcggc gaggtgatcg atccgcacac cgccgacgcc ctcaaggtcg
     1261 cgcgcgagca cgccgaagcg gacacgccga tgatcgtcct cgagaccgcg ctgccggcga
     1321 aattcagcga gacggtgcgc gaggcgatcg gcggcgagcc gcctcggccg gcgcgcttcg
     1381 acggcatcga gtcgctgccg cgccgcttcg agaccatgcc gcccagcctg tcgcggctga
     1441 aggcgttcat cgccgaaaat gactgaaccg tgagcactgc ctcgcccgcc ccggccacgc
     1501 cgccgcttct gtcgctcgac gacgccgtgg cgcagatgct ggcgcgcgtc cagatcccgg
     1561 cagccgatcg ggccgaggag gtgtcggcgt tcgacgccct gcacagggtt ctcgccgtcg
     1621 atgtccgttc gcacgtcgac gttccccccg ccgacaacag cgggatggac ggctacgcct
     1681 tgcgaagcgc cgatgtgccg gtcgccggaa cggtgctgcc cgtcagccag cggattccgg
     1741 ccggcgtcgt cggcagcgcc ctcgagcccg gcacggcggc gcgcatcttc accggcgcac
     1801 cgattcccgc cggggccgat gccgtggtca tgcaggagaa atgcggcgtc gtcgacggtg
     1861 ccgttcgtat cgacgtcgtg cctgccgccg gccagtcggt gcggcggcgc ggcgaagacg
     1921 tccgccgagg ctcgacggtg ctcgtcgccg gcacccggct gacgccgcag gcgctcggca
     1981 tggcggcatc ggtgggcgtc ggcaagcttc gcgtcggccg gcgtccgcgg gtggccctgt
     2041 tctccacggg cgacgagctg gcgatgcctg gcgacgtgct cgagccgggt gccatctaca
     2101 actccaaccg cttcacgctg cgcggcctga tcgaggcgct cggcggtgca tgcgaggatt
     2161 tcggcatcgt ccccgatcgt ctcgacgcga cgcgcgagac gctcaggcgt gctgccgaag
     2221 gcaacgacct cgtcgtcacg tgcggcggca tgtcggtggg cgaggaagac cacatgaagc
     2281 cggcggtcga gaaggagggc cgtctcgacc tctggcagat cgcgatgaag ccgggcaagc
     2341 cgctcgcttt cggcgaggtg tttcgccgcg acggctcgtc ggcctggttc gtcggcctgc
     2401 ccgggaatcc cgtgtcgtcg ctggtgacct tcctgctcgc ggtccgtccg gtgatcctga
     2461 agctgcaagg ggcgagcgac ctggcgccgc gtccgatcgc gatgcgcgcc gatttttcat
     2521 ggcctcgccc ggaccggcgc cgtgagttcc tccgggtccg gcgcgacgcc accggcggcc
     2581 tcgatctgtt cgcgcaccag ggctcggcgg tgctgacctc gaccgtctgg gccgatggcc
     2641 ttgtcgacaa tcctgccggc gcggccattg ctccaggcga cttcgttcgc tacctcgcct
     2701 tcgccgagtt gctcgcttga agatcgagat ccgctatttc gcgtccatcc gtgaggcgct
     2761 cgggccggcg gaatccgtcg aggtggccgc cggcagcgac gtcgccgcgc tgcgcgactc
     2821 gctcgtcgca cgcggcggca gccatgccga ggcgctcggc cgcgcgcggc tcttgcgcag
     2881 tgcccgcaat cgggtcctgt gcgccgagtc ggcgttgctc gacgacggcg acgaggtcgg
     2941 gttcttcccc ccggtcaccg gcggatgaga tgagccgaca cgtccgcatc caggtcgagg
     3001 atttcgacat cggcgccgag gtcgccgcgc ttcgcgccgg cgacggtggc gtcggcgccg
     3061 tcgccgtctt catcggcacc gtgcgggacc gcgccggcgc cgaggcgatc gcgtcgctgg
     3121 agctcgagca ctatcccggc atgaccgagg cggcgatcga gtcgatgatc gacgcttcct
     3181 tcgcccggtt cgatctgcgt ggcgcacggg tcgtgcaccg cgtcggccag ctcgccccgc
     3241 aggcgcagat cgtccttgtc gcggtcgcct cggcgcatcg cggcgaggcc tttcaggcgt
     3301 gtgaattcct gatggactac ctgaagaccc aggcgccgtt ctggaagaag gagtcgtcgc
     3361 catcgggtgc gcgatgggtc gacgcccgca ttgccgacga cgcggcgctg gcccgttggg
     3421 gcatcgtctc cggcaacgcc ctcgcgccgc ccgaagcgtc gccgccatga cccgggtgca
     3481 atggcctcga cgctgaccgg gctgaatatc
//
LOCUS       DASRWS010000667         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2559303, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000667 DASRWS010000000
VERSION     DASRWS010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559303"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFF72_12785"
     CDS             complement(<1..1175)
                     /locus_tag="VFF72_12785"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HZV94087.1"
                     /translation="MAAALVLAMHGGAFANPQGASIVSGQASITTAGNTLSIANTPGT
                     IINWQGFSIAADEVTRFIQQGASSAVLNRVVGQDPSSLLGRLQSNGRVFLINPNGITF
                     GAGARIDVAGLVASTLALGNADFLNGNLRFAGAASAGPLAQLGAITTPSGGQVVLIAP
                     RVENSGVITTPQGEILLAAGQTVQLADTADPALRVTVTAPAGEALNLGRLIADGGRIG
                     IQAGLIAQNGTISADRAEVGTGGEVYLKASGAITLGAASRISANGRSGGQVTVDAGDG
                     SLQASGSLAATGSSAGGGSVRLLGSTVRVADAQVDASGGSAGGEILVGGDRQGANPAI
                     RDAQTTDIDAATTLRADADAAGNGGKVVVWSDQSTVFLGKLSARGGAQSGDGGFAEVS
                     "
     gene            complement(1460..>3161)
                     /gene="recG"
                     /locus_tag="VFF72_12790"
     CDS             complement(1460..>3161)
                     /gene="recG"
                     /locus_tag="VFF72_12790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZV94088.1"
                     /translation="MVHPVCKVVGPDAPLPTALTPVYPSTAQLSQAALRKAVGAALAH
                     APLDEIVPVGLMPARLPPLRDALARLHRPAPGLDAAALEDRSHPAWERLRFDELLAQQ
                     LSQLQAKRERETQRAPPFPARRGGVRDALLAQLPFRPTPAQRRVEGEIAADLARHVPM
                     HRLLQGDVGSGKTVVAALAAAVVIDAGWQCALMAPTEILAEQHLKKLVGWLEPLGVKV
                     AWLTGSRKGKARARMLEQVASGEASLVVGTHAVIEEQVRFARLGLAIVDEQHRFGVSQ
                     RLALRGKLATGDSGDAMEPHLLMMSATPIPRTLAMTLFADLDVSTIDALPPGRTPIET
                     RLFSDARREQVIARIGDEVARGRQVYWVCPLVEASAALELQDATATHAQLCAALPGRL
                     IGLLHGRMAAAEKTAVMSLFEKGEMAVLVATTVIEVGVDVANASLMVVEHAERFGLSQ
                     LHQLRGRVGRGPTASVCVLLYTAPLSDAAKARLKALLETGDGFEIARRDLEIRGPGEI
                     LGARQSGAALLRFADPVADEALLLEARRVAERLLDSAPDAAQAHVDRWLGGRAHYLNA
                     "
BASE COUNT          421 a         1112 c         1169 g          459 t
ORIGIN      
        1 gagacttcag cgaaaccgcc atcgccactc tgggcgccgc cgcgcgcgct cagcttgccg
       61 aggaataccg tggattgatc cgaccagacg acgaccttgc cgccgttgcc cgccgcgtcg
      121 gcgtcggcgc gcagcgtcgt cgccgcgtcg atgtcggtcg tctgcgcgtc gcggatcgcc
      181 ggattcgcgc cttgacggtc gccgccgacg aggatctcgc cgccggccga gccgcccgat
      241 gcgtcgacct gggcgtcggc cacccgcacg gtgctgccga gcaggcgaac cgagccgccg
      301 ccggcgctgc ttccggtggc cgcgaggctg cccgaggcct gcagcgatcc gtcgccggcg
      361 tcgaccgtga cctggccgcc cgatcggccg ttggcgctga tccggctggc cgcgccgagc
      421 gtgatcgccc cgcttgcctt caggtacacc tcaccgccgg tgccgacttc ggcgcggtcg
      481 gcgctgatcg ttccgttctg cgcgatcagt cccgcctgga tgccgattcg gccgccgtcg
      541 gcgatcagtc gtccgaggtt gagggcttcg ccggccggcg ccgtgacagt cacccgcaag
      601 gccggatcgg cggtatcggc aagctgcacg gtctggccgg cggcgagcag gatttcgccc
      661 tggggcgtcg tgatgacgcc gctgttctcg acgcgaggtg cgatgagcac cacctggccg
      721 ccggaaggcg tggtgatggc gccgagctgg gccagcgggc cggcgctcgc cgcgcccgcg
      781 aagcggaggt tgccgttcag gaaatcggcg ttgccgagag ccagcgtcga ggcgacgagg
      841 ccggcgacgt cgatccgcgc gccggcgccg aaggtgatgc cgttcggatt gatgagaaag
      901 acgcggccat tggactgcag gcgaccgagc aggctggacg ggtcctgacc gacgacgcga
      961 ttgagcacgg cgctgctggc gccttgctgg atgaagcgcg tcacctcgtc ggcggcgatc
     1021 gagaagccct gccagttgat gatcgtgccg ggcgtgttgg cgatggagag cgtgttgcca
     1081 gcggtcgtga tgcttgcctg gcccgagacg atcgaggcgc cttgcggatt ggcgaacgcg
     1141 ccaccatgca tcgcgagcac gagcgcggcg gccacgcctg aaaggcacgg cgcggcatgc
     1201 cgcggggggc ggcctggtcg acgtggtcgg atgggcagct ctgtcatcga tcgcgctcgt
     1261 cgaagtggcc ccggcgcgcc gggcgcgcgt ggcacgcggg ctccgcctcg caggcggctt
     1321 gttcgacagt ctcccggttt tgcggcgctt gccgcgccga cttgaattcg ttcgtcgggc
     1381 ttcggtgacc gactgcgcag ttcggcgcgc ttttgcggct tcgaaccgac agatcctgcg
     1441 ggtcgcaggc cgcccggggt caggcgttga ggtagtgcgc tcgtccgccg agccagcgat
     1501 cgacgtgggc ctgcgccgca tcgggcgcgc tgtccaggag acgctcggcg acgcggcgcg
     1561 cctcgagcag aagcgcctcg tcggcgaccg gatcggcaaa gcgcagcagc gccgcacccg
     1621 actgccgggc gccgaggatc tcgcccgggc cccggatctc gagatcgcgc cgcgcgatct
     1681 cgaagccgtc gccggtctcg agcaacgcct tcaggcgcgc cttcgctgca tcggagagcg
     1741 gcgcggtgta gagcaggacg cagacgctgg ccgtcgggcc ccggccgacg cggccgcgca
     1801 gctggtgcag ttgcgacagc ccgaatcgtt cggcgtgctc gacgaccatc agcgaggcgt
     1861 tggcgacgtc gacgccgacc tcgatcaccg tggtcgcgac gaggacggcc atctcgccct
     1921 tttcgaagag gctcatcacg gcggtcttct cggcggccgc catgcgaccg tggagcaggc
     1981 cgatcaggcg tcccggcagc gccgcgcaca gttgcgcgtg cgtcgcggtc gcgtcctgca
     2041 actcgagcgc ggccgacgct tcgacgagcg ggcagaccca gtagacctgc cgcccgcgcg
     2101 ccacctcgtc gccgatgcgg gcgatgacct gctcgcgccg ggcgtcggaa aacaggcgcg
     2161 tctcgatcgg cgtgcggccc ggcggcagcg cgtcgatcgt gctgacgtcg aggtcggcga
     2221 agagcgtcat cgccaaggtc cgcgggatgg gcgtcgcgct catcatgagc aggtgcggct
     2281 ccatggcgtc gcccgagtcg ccggtggcca gcttgccgcg cagcgccagg cgctggctaa
     2341 cgccgaagcg atgctgctcg tcgacgatcg ccaggcccag ccgggcgaag cggacctgct
     2401 cctcgatcac cgcatgggtg ccgacgacga gcgacgcttc gccggaggcg acctgctcga
     2461 gcatccgcgc gcgcgccttg cccttgcggc tgccggtgag ccacgcgacc ttgacgccga
     2521 gcggctcgag ccagccgacg agcttcttca gatgctgctc ggcgaggatc tcggtcggcg
     2581 ccatgagcgc gcattgccag ccggcgtcga tgacgacggc cgcggcgagc gcggccacca
     2641 ccgtcttgcc cgacccgacg tcgccttgca gcagccggtg catcggcacg tggcgggcga
     2701 ggtcggcagc gatctcgcct tcgacgcggc gctgcgccgg cgtcggacga aacggcagtt
     2761 gcgcgagcag tgcgtcgcgc actccgccgc ggcgcgccgg aaaaggcggt gcccgctgcg
     2821 tctcgcgctc gcgcttggcc tgcagctgcg aaagctgctg cgcaagcagc tcgtcgaagc
     2881 gcaggcgctc ccatgcgggg tgcgaccggt cttccagcgc cgctgcgtcg agaccgggcg
     2941 ccggacgatg cagtcgcgcc agcgcgtcgc gaagcggcgg caggcgtgcc ggcatgagcc
     3001 caaccggcac gatctcgtcc aggggggcgt gggccagtgc cgcgccgacc gccttgcgca
     3061 gcgccgcctg cgacagctga gccgtgctcg ggtacaccgg tgtcaacgcg gtcggcagcg
     3121 gcgcgtcggg tccgaccacc ttgcacaccg ggtgcaccat a
//
LOCUS       DASRWS010000668         6010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3999391, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000668 DASRWS010000000
VERSION     DASRWS010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3999391"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFF72_12795"
     CDS             <1..297
                     /locus_tag="VFF72_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HZV94089.1"
                     /translation="VGGIVGALLTGVFNSPTLGGPSFPDNWFTGELVKPENYSIAAQV
                     LTQLKAVAVTIAWSGVVSLVAYKVVDMTIGLRVSEEDEREGLDITSHGETAYHN"
     gene            443..1738
                     /gene="gshA"
                     /locus_tag="VFF72_12800"
     CDS             443..1738
                     /gene="gshA"
                     /locus_tag="VFF72_12800"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZV94090.1"
                     /translation="MVPHLITALTGPINELEQRILESLPAIERWFRLEWMEHTPPFYT
                     SVDLRNAGFKLAPVDTNLFPGGFNNLTTEMLPLAVQAAMAAIEKICPEAKNLLLIPEK
                     HTRNTFYLANVQRLIEIFHQAGLNVRLGTLDPAITSPTEVPLPDGSALTVEPLVRTGN
                     RLGLKNFDPCTILLNNDLSAGLPKILENLHEQYLLPPLHAGWAVRRKTNHFAAYEEVA
                     KKFAKLLGMDPWLINPMFARCGEVNFSDGSGAECLTSNAETLLGRIRRKYKEYGIQEK
                     PFIIVKADAGTYGMGIMTVRDPKELADLNRRSRNKMSVIKDGQEVSEVILQEGVPTFE
                     RVNDSVAEPVVYMIDRYVVGGFYRVNPERGNDENLNSPGASFVPLAFAGSNQLPKPGS
                     KPGVSAPNRFYMYGVIARLAMVAASYELEATDPDAEIYE"
     gene            1842..3716
                     /locus_tag="VFF72_12805"
     CDS             1842..3716
                     /locus_tag="VFF72_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HZV94091.1"
                     /translation="MSHPRTASSLFALTLGAIGVVYGDIGTSPLYALKEVFAHGHVPL
                     TAHNIYGVLSLVFWTLTVVVSIKYVVLILRADNNGEGGLIAMLALASQAVKDRPKLRR
                     RLLFLGIFGTAIFFGDGVITPAISVLSAVEGLQVAAPGLHSWVMPITLVVLAGLFVFQ
                     RHGTARVGRLFGPVMAMWFLALAALGLAHIGDNIAILKALSPHYALLFMIGHPSVGFI
                     ALGSVVLCVTGAEALYADMGHFGKRPIRMAWFGLAMPALVINYFGQGAMLLAYPGKVG
                     NPFFEMAPAWALYPLIVLATCATVIASQALITAAFSVTKQAIQLGYLPRLRVLHTSVL
                     ETGQIYVPFVNWGLFCFIVLAVVLFGSSDALAAAYGITVTIDMLITTTMTFFVVRYGW
                     KYPWPLTVAATGFFFIVDFTYFSANVVKVFDGGWFPILIGAMMFTLMMTWKQGRRLMS
                     ESLRSDALDLKSFLESVFISPPARVPGTAVFLVSDQGLTPNALLHNLKHNKVLHERNL
                     FVTVRQHERPWIAKSERCDIEPLGNECWQVTLNFGFKDEPDVPEALERLRSSGCVIDD
                     MDTSYFLSRDIVIPTMGGGMADWREKLFAGMHRNASAAADYLRLPTNRVVELGSKVEI
                     "
     gene            3820..5010
                     /locus_tag="VFF72_12810"
     CDS             3820..5010
                     /locus_tag="VFF72_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HZV94092.1"
                     /translation="MSDVSLTAVAAGFVAVLVGFTSSVAIVFQAATSLGATPAEAASW
                     MWALGWGMGLTTLVPSLWLRKPVMIAWSTPGAAVLAVAGANGGFGMAEAIGAFIVCAL
                     LIVVVGVSGAFERAMSKIPTPLAGALLAGVLARFGLDAFSALQTSFALVIAMLVAYLL
                     ARRWLPRYAVLVVLLAGIAVAAGEGRLHPGAVQWTPTMPVFTMPSFTWRAAVSLALPL
                     FIVTMASQNLPGVAVIRATGYRLPISRLITMTGVATLLLAPFGAFSLNLAAITAAICM
                     GREAHADPSRRWVAAAACGALYCVVGTFGAAVAGVLAAFPRDLIIAVAGIALLATIGS
                     GLASALADDHCREAAIITFLVTLSGLSLAGVGSAFWGVAAGALALFVQQWRPRIAASR
                     SRSP"
     gene            5007..5969
                     /gene="gshB"
                     /locus_tag="VFF72_12815"
     CDS             5007..5969
                     /gene="gshB"
                     /locus_tag="VFF72_12815"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZV94093.1"
                     /translation="MRLLFVADPLESFNVKKDTTFAMMREAARRGHALFACEVRDLMW
                     ERGARVASHVREIALTGRKPDWFNETSVDPAAPRRRVALADFDAVLMRKDPPFDSEYF
                     YATHLLEQAEREGARVFNRPAALRDHPEKLAILEFPQFISPTLVTRDPAELRRFHAEH
                     ADVIFKPLDGMGGTGIFRVGADGMNLGVVVETLNARGSQTVMAQKYVPEIVRGDKRVL
                     VIAGEAVPMVLARIPQGQEIRGNLAAGGKGVAQPISARDREIAAHIGPILAARGLLLV
                     GLDVIGDCLTEINVTSPTGFQEITEQSGIDVAKIYVDALEAALR"
BASE COUNT          887 a         2129 c         1951 g         1043 t
ORIGIN      
        1 gtcggcggca tcgtcggggc gctgctgacc ggcgtcttca actcgccgac gctcggcggc
       61 ccgagctttc ccgacaactg gttcaccggc gagctggtca agcccgagaa ctactcgatc
      121 gccgcccagg tcctcaccca attgaaggcg gtggccgtca cgatcgcctg gtcgggcgtc
      181 gtctcgctgg tcgcctacaa ggtcgtcgac atgacgatcg gcctgcgcgt cagcgaggaa
      241 gacgagcgcg aagggctcga catcacctcg cacggcgaga cggcgtacca caactgagcc
      301 gtcgccttgc gaaaccccgg ggccgccgcg aggcggccct ttttcatggc gcagccggcc
      361 tgccttgcga ccggcgggcg gcgccccata tgcctagaat cccacctccc ccgcacgccc
      421 ggcgctcgcc tcgggccttc gcatggttcc tcatctcatc accgccctga cgggcccgat
      481 caacgagctc gagcagcgca ttctcgaatc gctgccggcg atcgagcgct ggtttcggct
      541 cgagtggatg gagcacacgc cgccgttcta cacgtcggtc gacctgcgca atgccggctt
      601 caagctggcg cccgtggaca ccaacctctt tccgggcggc ttcaacaacc tgacgacgga
      661 aatgctgcct ctggcggtgc aggcggcgat ggcggcgatc gagaagatct gccccgaggc
      721 gaagaacctg ctgctgatcc ccgagaagca cacgcgcaac accttctacc tcgccaacgt
      781 gcagcggctg atcgagatct tccatcaggc gggcctgaac gtgcgcctgg gcacgctcga
      841 cccggccatc acctcgccca ccgaggtgcc gcttcccgac ggcagcgccc tcacggtcga
      901 gcccctggtg cgcaccggaa accgcctggg cctgaagaat ttcgacccct gcaccatcct
      961 gctcaacaac gatctctcgg ccggcctgcc gaagatcctc gagaacctgc acgagcagta
     1021 cctgctgccg ccgttgcacg ccggctgggc ggtgcggcgc aagaccaacc atttcgccgc
     1081 ctacgaggag gtggcgaaga agttcgccaa gctgctcggc atggacccct ggctcatcaa
     1141 cccgatgttc gcgcgctgcg gcgaggtgaa cttcagcgac ggctcgggcg ccgaatgcct
     1201 gacgagcaat gccgagacgc tgctcggccg gatccgccgc aagtacaagg aatacggcat
     1261 ccaggaaaag cccttcatca tcgtcaaggc cgacgccgga acctacggca tgggaatcat
     1321 gacggtgcgc gacccgaagg agctcgccga cctgaaccgg cgctcgcgca acaagatgag
     1381 cgtcatcaag gacggccagg aggtgagcga ggtcatcctc caggaaggcg tgccgacctt
     1441 cgagcgcgtc aacgattccg tcgccgagcc cgtcgtctac atgatcgacc gctacgtcgt
     1501 cggcggcttc tatcgcgtca accccgagcg cggcaacgac gagaacctca attcgccggg
     1561 ggcgagtttc gtgccgctgg ccttcgcagg ctcgaaccag ctgcccaagc ccggcagcaa
     1621 gccgggtgtc agcgcgccga accggttcta catgtacggc gtgattgccc ggctcgcgat
     1681 ggtcgcggcg agctacgagc tcgaagccac cgatcccgac gccgaaatct acgagtagtg
     1741 cccgagctgc cggccgcgag cgcggccgag gcgacgcaag ggcacaatag cgcctctttt
     1801 tcgccgccgc cgcgcctgtc tgcgcgcccg ccggctccag cgtgagtcat ccccgcaccg
     1861 cctccagcct gttcgctctg acgctcggtg ccatcggcgt cgtctacggc gacatcggca
     1921 cgagtccgct ctatgcgttg aaggaggtct tcgcccacgg ccacgttccg ctcaccgcgc
     1981 acaacatcta cggcgttctc tcgctcgtct tctggaccct cacggtcgtc gtctcgatca
     2041 agtacgtcgt cctgatcctg cgcgccgaca acaacggcga gggcggcctg atcgccatgc
     2101 tggcgctcgc ttcgcaagcg gtgaaggaca ggccgaaact gcgccgccgc ctgcttttcc
     2161 tcggcatctt cgggacggcg atcttcttcg gagacggcgt catcacgccg gcgatctccg
     2221 tcctctcggc ggtcgagggc ctgcaggtcg cggcgcccgg actgcactcc tgggtcatgc
     2281 cgatcacgct ggtcgtcctc gccgggctgt tcgtcttcca gcggcatggc acggcgcgcg
     2341 tcggtcgcct gttcgggccg gtgatggcga tgtggttcct ggcgctcgcg gcgctcgggc
     2401 tcgcccacat cggcgacaac atcgccatcc tgaaggcgct gtcgccgcac tacgcgctgc
     2461 tcttcatgat cggccatccc tcggtcggct tcatcgccct gggctcggtc gtcctctgcg
     2521 tcaccggcgc cgaggcgctc tacgccgaca tgggccactt cggcaagcgg ccgatccgca
     2581 tggcctggtt cggcctggcg atgccggcgc tggtcatcaa ctacttcggc cagggcgcga
     2641 tgctgctggc ctatccgggc aaggtcggca atcccttctt cgaaatggcg cccgcctggg
     2701 ccctgtaccc gctgatcgtg ctcgccacct gcgccacggt gatcgcttcg caggcgctca
     2761 tcacggcggc gttctcggtg accaagcagg cgatccagct cggctacctg cccaggctgc
     2821 gcgtgctgca tacctccgtg ctcgagaccg gccagatcta cgttccgttc gtgaactggg
     2881 ggctcttctg cttcatcgtc ctcgcggtcg tcctgttcgg ctcgagcgac gcgctggccg
     2941 cggcctacgg catcacggtg acgatcgaca tgctgatcac gacgacgatg accttcttcg
     3001 tcgtgcgcta cggctggaag tacccgtggc cgctgaccgt cgccgcgacc ggcttcttct
     3061 tcatcgtcga cttcacctat ttctccgcca acgtcgtcaa ggtgttcgac ggcgggtggt
     3121 tcccgatcct gatcggcgcg atgatgttca cgctgatgat gacgtggaag caggggcgac
     3181 gcctgatgtc cgaaagcctg cgttcggacg cgctcgacct gaagagcttc ctcgaatcgg
     3241 tcttcatcag tccgccggcg cgggtgcccg gcaccgccgt tttcctggtg agcgaccagg
     3301 gcctgacgcc caatgcactg ctgcacaacc tcaagcacaa caaggtgctg cacgagcgca
     3361 acctgttcgt caccgtgcgc cagcacgagc ggccgtggat tgccaagagc gaacgctgcg
     3421 acatcgagcc gctgggcaac gagtgctggc aggtgacgct gaacttcggc ttcaaggacg
     3481 agcccgacgt gcccgaagcg ctcgagcgat tgcgctcgag cggatgcgtc atcgacgaca
     3541 tggacacgtc gtacttcctg tcgcgcgaca tcgtcatccc gacgatgggc ggtggcatgg
     3601 ccgactggcg cgagaaactc ttcgccggca tgcaccgcaa cgcctcggcc gccgccgact
     3661 atctgcgcct gccgaccaat cgggtggtcg agctgggctc gaaggtcgag atctgacggc
     3721 cactgcgcgt tcgcgtcggt cgccgagccc aggcggcgcc gggcttcggt cacgaccggc
     3781 aagtcgcccg tccggtcgcc ggccgtgacg ggcccgcgcc tgtcggacgt gtccctgaca
     3841 gccgtggcgg ctggtttcgt cgccgtgctc gtcggcttca cgagctcggt ggcgatcgtc
     3901 ttccaggcgg cgacatcgct cggcgcgacg cctgcggaag cggcatcgtg gatgtgggcg
     3961 ctcggctggg gcatgggcct gaccacgctc gtgccgtcgc tgtggctgag aaagccggtg
     4021 atgatcgcct ggagcacgcc cggcgcggcg gtgctcgcgg tcgccggcgc caacggcggc
     4081 ttcggcatgg ccgaggcgat cggcgcgttc atcgtctgcg cgctgctcat cgtcgtcgtc
     4141 ggtgtcagcg gcgcgttcga gcgggcgatg tcgaagattc cgacgccgct ggccggagcg
     4201 ctgctcgccg gcgtcctggc ccgcttcggt ctcgacgcgt tctcggcgct gcagacgtcg
     4261 ttcgcgctgg tcatcgcgat gctcgtcgcc tatctcctgg cgcgtcgctg gttaccgcgc
     4321 tacgcggtgc tcgtcgttct cctggccggc atcgccgtcg ccgccgggga gggccggctt
     4381 catccgggcg ccgtgcagtg gacgccgacg atgcccgtgt tcaccatgcc gtcgttcacc
     4441 tggcgggccg cggtgagcct ggcgctgcca cttttcatcg tcaccatggc gtcgcagaac
     4501 ctgccaggcg tcgcggtgat tcgcgccacc ggctaccggc tgccgatctc caggctgatc
     4561 acgatgacgg gcgtcgccac cttgctgctg gcgccgttcg gcgccttcag cctgaacctg
     4621 gcggcgatca cggcggcgat ctgcatgggt cgcgaggccc acgccgaccc gtcgcggcgc
     4681 tgggtcgcgg cggccgcatg cggtgcgctc tactgcgtcg tcggcacctt cggcgccgcc
     4741 gtcgccggcg tgctggcggc gtttccgcgc gacctgatca tcgccgtcgc cggcatcgcc
     4801 ctgctggcga cgatcggcag cggcctggcg tcggcgctgg cggacgacca ctgtcgggaa
     4861 gcagccatca tcacgttcct ggtgacgctc tcgggcctca gcctggcggg cgtcggctcg
     4921 gcgttctggg gcgtcgccgc cggcgccctc gcgctgtttg tgcaacagtg gcggccccgc
     4981 attgccgcgt cgcgctcgcg ctcgccatga gacttctctt cgtcgccgat ccgctcgaaa
     5041 gcttcaatgt caagaaggac acgaccttcg cgatgatgcg cgaggcggcc aggcgcggcc
     5101 acgccctgtt cgcctgcgag gttcgcgacc tgatgtggga gcgcggtgcc cgggtcgcct
     5161 cgcatgtgcg cgaaatcgcg ttgacgggca ggaagccgga ctggttcaac gagacctcgg
     5221 tcgacccggc ggcaccgcga aggcgggttg cgctcgccga tttcgacgcc gtcctgatgc
     5281 gcaaggaccc gcccttcgac agcgaatatt tctacgccac gcacctgctc gagcaggccg
     5341 agcgcgaggg cgcgcgcgtc ttcaatcggc ccgccgcgct gcgcgatcac ccggaaaagc
     5401 tcgccatcct cgagtttccg cagttcatct cgccaaccct ggtgacccgc gatccggcgg
     5461 agctgcgacg tttccatgcc gagcacgcag acgtcatctt caagccgctc gacggcatgg
     5521 gcggcaccgg catctttcgc gtcggcgccg atggcatgaa cctcggtgtc gtcgtcgaga
     5581 cgctcaacgc gcgcggcagc cagaccgtga tggcgcagaa atacgtgccc gagatcgtcc
     5641 gcggcgacaa gcgcgtgctg gtcatcgccg gcgaggcggt gccgatggta ctggccagga
     5701 ttccgcaggg ccaggagatc cgcggcaacc tcgccgccgg cggcaagggc gtcgcccagc
     5761 cgatctcggc gcgcgaccgc gagatcgccg cccacatcgg gccgatcctc gcggctcgcg
     5821 gcctgctgct ggtcggcctc gacgtcatcg gcgactgcct caccgagatc aacgtgacga
     5881 gcccgaccgg cttccaggag atcaccgagc aaagcggcat cgacgtggcg aagatctacg
     5941 tcgacgccct cgaggccgct ctgcgctagc ggctccgatg gctgtcctgt cgctttccgg
     6001 ggcgcacctc
//
LOCUS       DASRWS010000669         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1413830, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000669 DASRWS010000000
VERSION     DASRWS010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413830"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFF72_12820"
     CDS             <1..808
                     /locus_tag="VFF72_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV94094.1"
                     /translation="AVALLLPMSALHAIDRESPLPLYAQVKRRLRAAILAWPAGNDVF
                     HTEHALCATFGVSRATVRQAVAELESEGLLRRRQGFGTLVNRQKIEESFTPLTNFRDQ
                     WASSGRVLKVELHRAERIACPAPFAEMLGLAAGEPVLHLERFRLSDGVRVAWDSRFIP
                     KAVAAGIARREFAKVSLLDVLARKVEFDRGESQLESALAGDEHADRLDLLPGDPVLMR
                     HLIYFSTDDRPVLAGLSVYRADQVRYKVSAPMRGRGVDADTELGALGVLV"
     gene            852..2021
                     /locus_tag="VFF72_12825"
     CDS             852..2021
                     /locus_tag="VFF72_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV94095.1"
                     /translation="MKAAFRVCAAVLMSLAAWALPGGAAWADGGVKIGSILSVTGPAA
                     FLGEDMKAGMELAIDEINAKGGVLGKKIDWVFYDAESQTQKGLTATRRLLDQDKVDMI
                     VGGGNMSGMAIAMLGLTEKAQLPFMSTEGAMDIVTPVAERQWTFKSTADDEQVMERLA
                     DYFAKKGIRKIAFLGDSSGFGQSAAVQLKKVAARRGLDVMYESFNPSDTDMTPQLGKI
                     KEANVQAIVCWTVTPAGVVFLKEAQQLGLDKLTLIHSYGFVDQRYMKLAGDAAKNLLL
                     VSVKFPVGADLPDSDPQKARIAALTKAFEAKFHRKPNQFAAQTYDAIYLAKTAFDRAG
                     STDHAKVRNALEGIHGWQGVGGVFNFSKEHHSGLSKDDLVLVDYHDGAFHLADYK"
     gene            2079..2996
                     /locus_tag="VFF72_12830"
     CDS             2079..2996
                     /locus_tag="VFF72_12830"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZV94096.1"
                     /translation="MSLTILDAPEARFPLRLGIENKRIRELFHNATRQQWNPATEIAW
                     DELHPEQYDDAHLRAARMYWSRRAWGEYGAISESPALQIRFCQEHCVPDMALFFSIRS
                     QEEARHAEVCWRMAEALGGYFEEPAATAFQGSVATHGIRKMALDPTISLEGTIAALVC
                     AAEEIAFDVFRHLIDITVNPVARHVTRMILRDEVRHCAFGWAYMEQRMPQLTADEREG
                     VRQAVITMIEKVELNGYHSSWLAPETPASSAEVEVDRLTWEAGLGATTEELEKPVFLK
                     SIAVMRERMAADWGLEIPMFRHRKFAAPF"
     gene            3009..3926
                     /locus_tag="VFF72_12835"
     CDS             3009..3926
                     /locus_tag="VFF72_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94097.1"
                     /translation="MQTSPHATSMPVERRFPLELQKADDDIRALYETAKKAHWNPQTD
                     IAWSAHGLAGVDRDTLDAARRVWSRRAWVEFTGMAETPALLIRFCLEMNRESDPKYFL
                     AVRNTEEAWHVECFHRYAEACGGYVERPATAAWEPVFNRTLYRDALDAEVSIDAYVAA
                     HCAFVDSLELALATAWLESAREPLARAILARCVGDYERHARFGMMYARRRAAAMDEAL
                     RGRVATALQRHVEQVEFAGYHCVGLSTEIDASAEAADLARVAAAGLGAVGADEEIAIF
                     RRCVARTRDAFATLGLALPPIAHRHLGEF"
BASE COUNT          649 a         1373 c         1352 g          636 t
ORIGIN      
        1 cgcggtcgcg ttgcttctcc cgatgtccgc cttgcatgcc atcgatcgcg aatcgccgct
       61 gccgctctac gcgcaggtga agcggcggct gcgagcggcg atcctggcat ggcctgccgg
      121 caatgatgtc tttcataccg agcatgccct gtgcgcgacc ttcggcgtga gccgcgccac
      181 ggtgcgccag gccgtggccg agctcgaaag cgaaggcctg ctgcgccgcc gccagggatt
      241 cggcacgctg gtcaaccggc agaagatcga agagtcgttc acgccgctca ccaacttccg
      301 cgaccagtgg gcgagcagcg gacgggtgct caaggtcgag ctgcatcgcg ccgaacgcat
      361 tgcctgcccg gcgcccttcg ccgagatgct gggcctggcc gccggcgagc cggtgcttca
      421 tctcgagcgc tttcgccttt ccgacggcgt tcgcgtcgcc tgggacagcc gcttcatccc
      481 gaaggcggtg gccgccggca tcgcccgccg cgagttcgcc aaggtgtcgc tgctcgacgt
      541 gctcgcccgc aaggtcgagt tcgaccgcgg cgaaagccag ctcgagtcgg ccctggccgg
      601 cgacgagcac gccgatcggc tcgatttgct gccgggcgat ccggtgctga tgcgccacct
      661 gatctacttt tcgaccgacg atcgacccgt gctggcggga ctgtcggtct atcgggccga
      721 ccaggtccgc tacaaggtgt cggcgccgat gcgcggccgc ggcgtcgatg cggacaccga
      781 gctcggcgcc ctcggcgtgc tcgtctgacg tccgagattt ccttcggtcc actgccttcg
      841 aaaggtcccc catgaaagct gcattccgtg tttgtgccgc cgtgctgatg tcgctggcag
      901 cctgggcgct gcctggcggc gcggcctggg ccgacggggg cgtgaagatc ggctcgatcc
      961 tgtcggtcac cgggccggcg gcctttctcg gcgaggacat gaaggccggc atggagctcg
     1021 cgatcgacga gatcaatgcc aagggaggcg tcctcggcaa gaagatcgac tgggtcttct
     1081 acgacgccga aagccagacc cagaagggcc tgacggcgac ccgccggctg ctcgaccagg
     1141 acaaggtcga catgatcgtc ggcggcggca acatgagcgg gatggcgatc gcgatgctcg
     1201 gcctgaccga gaaggcgcag ctgccgttca tgtccaccga aggcgcgatg gacatcgtca
     1261 cgccggtggc cgagcggcag tggaccttca agagcacggc cgacgacgag caggtgatgg
     1321 agcggctggc cgactacttc gcgaagaagg gcatcaggaa gatcgccttt ctcggcgact
     1381 ccagcggctt cggccagagc gccgcggtgc agctgaagaa ggtcgcggcg cgacgcggcc
     1441 tcgacgtgat gtacgagtcg ttcaatccgt cggacaccga catgacgccg cagctcggca
     1501 agatcaagga ggcgaacgtg caggcgatcg tctgctggac ggtgacgccc gcgggggtcg
     1561 tcttcctgaa ggaggcgcaa cagctcggcc tcgacaagct caccctgatc cacagctacg
     1621 gcttcgtcga ccagcgctac atgaagctgg ccggcgacgc ggcgaagaac ctgctcctgg
     1681 tctcggtgaa gttcccggtc ggcgccgacc tgcccgacag cgatccgcag aaggcgcgca
     1741 tcgccgcgct caccaaggcc ttcgaggcca agttccacag gaagcccaac cagttcgcgg
     1801 cgcagaccta cgacgcgatc tatctggcca agaccgcctt cgacagggcc ggcagcaccg
     1861 accacgccaa ggtgcgcaac gccctggaag gcatccatgg ctggcagggc gtgggcggcg
     1921 tcttcaactt cagcaaggaa caccactcgg gcctgtcgaa ggacgacctg gtgctggtgg
     1981 actatcacga cggcgcgttc catctcgccg actacaagta ggggcgtggc gcgaatgcgc
     2041 gacggcggcg cggcagcgcc atctgcaagg gaattgccat gagcctgacg atcctcgacg
     2101 cgcccgaggc gcgctttccg ctgcgtctgg gcatcgagaa caagcgcatt cgcgagctct
     2161 tccacaacgc cacgcgccag cagtggaacc cggcgaccga gatcgcctgg gacgagctcc
     2221 atcccgagca gtacgacgac gcgcacctgc gcgccgcgcg catgtactgg agccggcgcg
     2281 cctggggcga gtacggtgcc atctcggaga gcccggcgct gcagatccgc ttctgccagg
     2341 agcactgcgt gcccgacatg gcgctcttct tcagcatccg ctctcaggaa gaagcgcgcc
     2401 acgccgaggt ctgctggcgg atggccgagg ccctcggcgg ctatttcgag gagcctgcgg
     2461 ccaccgcgtt ccagggctcg gtggccaccc acggcatccg caagatggcg ctcgatccga
     2521 cgatctcgct cgagggcacg atcgcggcgc tggtctgcgc cgccgaggag atcgccttcg
     2581 acgtctttcg ccacctgatc gacatcaccg tcaacccggt cgcccgtcac gtcacccgga
     2641 tgatccttcg cgacgaggtc cgccattgcg ccttcggctg ggcctacatg gagcagcgca
     2701 tgccccagct caccgccgac gagcgcgaag gcgttcgcca ggcggtcatc acgatgatcg
     2761 agaaggtcga gctcaacggc tatcacagct cgtggctcgc tcccgagacg ccggcttcgt
     2821 cggccgaggt cgaggtcgac cggctgacct gggaggcggg actcggcgcc accaccgaag
     2881 agctcgaaaa gcccgtgttc ctgaagtcga tcgcggtgat gcgcgagcgc atggccgccg
     2941 actggggcct cgagatcccg atgttcaggc atcgcaagtt cgctgccccc ttctgaggcg
     3001 ggatcgacat gcagacatcg ccccacgcca cgtcgatgcc ggtcgagcgg cggttcccgc
     3061 tcgaactgca gaaggccgat gacgacatcc gcgccctcta cgaaaccgca aagaaggcgc
     3121 actggaatcc gcagaccgac atcgcgtggt cggcgcacgg gcttgccggc gtcgatcgcg
     3181 acacgctcga cgcggcgcgc cgggtctgga gccgccgcgc ctgggtcgaa ttcaccggca
     3241 tggccgagac gccggccctc ctcatccgct tctgtctcga gatgaatcgc gagtcggacc
     3301 cgaaatactt cctggcggtg cgcaacaccg aggaggcctg gcacgtcgag tgtttccatc
     3361 gctatgccga agcgtgcgga ggctacgtcg agcggccggc gaccgccgcc tgggagccgg
     3421 tgttcaatcg cacgctctat cgcgacgcgc tcgatgccga ggtctcgatc gacgcctacg
     3481 tcgcggcgca ctgcgccttc gtcgacagcc tcgagctggc gctcgccacg gcgtggctgg
     3541 aaagcgcccg cgagccgctg gcgcgcgcca tcctcgcccg ctgcgtcggc gactacgagc
     3601 gccatgcccg cttcggcatg atgtacgcac ggcgccgtgc cgcggcgatg gacgaagcgc
     3661 tgcgcggccg cgtcgcgacg gcgttgcagc gccatgtcga gcaggtcgag ttcgccggct
     3721 atcactgcgt cggcctgtcg accgagatcg atgcatcggc cgaggcggcc gatctggccc
     3781 gtgtcgcagc ggcggggctc ggcgcggtcg gcgccgacga ggagatcgcg atctttcgtc
     3841 gctgcgtcgc ccgaacccgc gatgccttcg cgaccctcgg cctggcgttg ccgccgatcg
     3901 cgcatcgtca tctcggcgag ttctaggggc ctgcgtggag ctgggcaccc tgctgctctc
     3961 ggggttgtcg acgggcagca tctatgcgct cgtcgctctc ggcttcaacg
//
LOCUS       DASRWS010000670         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2560530, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000670 DASRWS010000000
VERSION     DASRWS010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560530"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFF72_12840"
     CDS             complement(<1..1247)
                     /locus_tag="VFF72_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZV94098.1"
                     /translation="MTEVVVTLKAPPLSTFGRSLLSARHVDYLHRLESQQAVVSTRIE
                     QTIPSSRIRWRYKLVANGLAVVLPRSQVAELTHVPGVAEVWPNVRYHSLAAELVPQQI
                     GADKLWGPSFETAGNGMKIGIIDDGVEAAHPYFNPSGFQYPPGFPKGQTQFATPKVIV
                     QRTFTPPSPTWKYASAPFDPEYSFHATHVAGIAAGVHGTQDGPDTLSGVAPNAYIGNY
                     KALTIPTPGFGLDGNSAELAAAIEAAVADGMNVINLSLGEPEVEPSRDLVVKALEGAA
                     AAGVVPVIAAGNDFDALGFGSIDSPGSAPKAITAAAATKEGAIASFSSAGPTPYSLRL
                     KPDVTAPGVSILSSVPARYGSWREFDGTSMAAPHVAGAAALLRQRHPGWSVSQVKSAL
                     VTTGNSVAANGRQAPPTRVGGGM"
     gene            complement(1504..1758)
                     /locus_tag="VFF72_12845"
     CDS             complement(1504..1758)
                     /locus_tag="VFF72_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94099.1"
                     /translation="MKEQLRLEVGDSYGHRLDLGGQRSRPRHTEVVRECEHPLGEPVV
                     LAKQVALTMLQMRELGRQRLGIWELLDRHDCSYRQRRAGT"
     gene            complement(1778..>2214)
                     /locus_tag="VFF72_12850"
     CDS             complement(1778..>2214)
                     /locus_tag="VFF72_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94100.1"
                     /translation="VPCFTARRVTPATARESYLCAPRPSGCFAVGTGNSAFWHRYARD
                     AYAIWVLAHEAIHAGQAVKGAPQVADNVVEVQADCYGLQWIPSVATQLGDRLDDAEAI
                     ASYDWLIQYPQKSREPPRPYWSSACHPGGALDIRAAGSTFWP"
BASE COUNT          323 a          771 c          787 g          333 t
ORIGIN      
        1 atcattccgc cgcccacgcg ggtcggcggc gcctgccgcc cgttcgcggc gactgagttg
       61 cccgtcgtga cgagcgcgga cttgacctgg ctgacgctcc agcccgggtg ccgctggcgc
      121 aagagcgccg cagcgcccgc cacgtgcggc gcggccatgc tcgtgccgtc gaactcacgc
      181 cagctgccgt agcgggcagg cacggacgag aggatcgaga cccccggggc ggtgacgtcc
      241 ggcttcaagc gcaaggagta gggcgtcggg cctgcggacg agaagcttgc gatcgcgccc
      301 tccttcgtcg ccgctgccgc cgtgatcgct ttcggcgccg agccgggcga atcgatcgag
      361 ccgaacccga gcgcgtcgaa gtcgttgccc gccgcgatca ccgggacgac gccggcggct
      421 gcggcgcctt cgagcgcctt caccacgaga tcgcgcgacg gctcgacctc gggctcgccg
      481 agcgagaggt tgatcacgtt catcccgtct gcgaccgccg cctcgatcgc ggccgcgagc
      541 tcggcgctgt tgccgtcgag accgaagccg ggcgtcggga tggtgagcgc cttgtagttg
      601 ccgatgtacg cgttcggcgc gacacccgag agcgtgtcgg gaccgtcctg ggtgccgtgc
      661 acgccggccg cgattcccgc cacgtgagtc gcgtggaacg aatactcggg gtcgaacggt
      721 gctgatgcgt acttccacgt cggcgaaggc ggcgtgaacg tgcgctggac gatcaccttc
      781 ggcgtcgcga attgcgtctg gcccttcgga aagccggggg gatactggaa gccgctcggg
      841 ttgaagtacg gatgggcggc ctcgacgccg tcgtcgatga tgccgatctt catgccgttg
      901 cccgccgtct cgaagctcgg cccccagagc ttgtcagcgc cgatctgctg cgggacgagc
      961 tcggcggcga gcgagtggta gcgcacgttc ggccacacct cagcgacgcc cgggacgtgc
     1021 gtgagctcgg cgacctggct gcgcggcagg acgaccgcga ggccgttcgc gacgagcttg
     1081 tagcgccagc ggatccgcga cgacgggatc gtctgctcga tccgtgtgga gacgaccgcc
     1141 tgctgcgact ccagccgatg cagatagtcg acgtggcgcg cggacagcag tgagcgtccg
     1201 aacgtcgaaa gcggcggcgc cttcagcgtc acgacgacct cggtcatcgg cacgggcgtc
     1261 cgtccgaatc cgtcccccga cagcgcgatc gcagacgccg ccgacaccac gatgatcgtg
     1321 agaaggcgtc tcagagtgca gcatcgtagc cgcggttcct gcgcttacgc ggcggggcgg
     1381 cggaccgggg gagagttcgg ccgccgccgc cgccgcggcc ggcatccggc tccccctcgg
     1441 gcctgagcga cggatgccga gacctgtctt cgcgcccgac cggcgtcgtc ctacttctcg
     1501 tgctcaggtg cccgcccggc gctgccgata cgagcagtcg tggcggtcga gaagctccca
     1561 gataccgagg cgctggcggc cgagctcgcg catctgcagc atcgtcagcg cgacctgctt
     1621 cgccagcacg acaggctcac cgagcggatg ctcgcattcc cgaacgactt cggtatgacg
     1681 tgggcgcgat cgctggccgc cgagatcgag gcgatggccg tacgaatcgc cgacctcgag
     1741 gcgcagttgc tctttcatcg ctagcgtcgt cgcgcggtca gggccagaac gtcgagcccg
     1801 cggcgcgtat gtccagcgcg ccgcccggat ggcaggcgga cgaccagtac ggcctgggcg
     1861 gctcgcgcga cttctggggg tactggatca gccagtcgta gctcgcgatc gcctcggcgt
     1921 cgtcgagccg gtctccgagc tgcgtcgcga ccgacgggat ccattgcagg ccgtagcagt
     1981 cggcctgcac ctcgaccacg ttgtcggcga cctgcggcgc gcccttgacc gcctgtccgg
     2041 cgtgaatcgc ctcgtgggcc agcacccaga tcgcatacgc gtcccgcgcg tagcgatgcc
     2101 agaaggcgct gttgccggtc ccgaccgcaa agcagcccga cgggcggggc gcgcacagat
     2161 acgactcgcg cgccgtcgca ggcgtgaccc ggcgtgcggt gaagcacggc accg
//
LOCUS       DASRWS010000671         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_271773, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000671 DASRWS010000000
VERSION     DASRWS010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271773"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /gene="xsc"
                     /locus_tag="VFF72_12855"
     CDS             complement(<1..1628)
                     /gene="xsc"
                     /locus_tag="VFF72_12855"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HZV94101.1"
                     /translation="MNRDPLPAADTRKPVQGVQKMTPSEAFVETMVANRVTDIFGIMG
                     SAFMDAMDIFAPAGIRLIPVVHEQGAAHMADGYARVSGRHGVVIGQNGPGISNCVTAI
                     AAAFWAHSPVVMITPETGTMGMGLGGFQEANQLPMFQEFTKYQGHVNNPRRMAEYTAR
                     CFDRAISEMGPTQLNIPRDYFYGEITVEIPKPMRVERGAGGEQSLNEAADLLAKAKFP
                     VILAGGGVVMGDAVEETKALAERLGAPVVTGYLRNDAFPASHPLWCGPLGYQGSKAAM
                     KLIAQADVVVALGSRMGPFGTLPQHGLDYWPKDAKIIQVEADHTNLGLVKKISVGIAG
                     DAKAAAAAISKRLEGRSLACDASKAERAKKVADEKAAWEKELTEWTHEKDPYSLDMIE
                     EQKKEEGRWLHPRQVLRELEKAMPAHVMVSTDIGNINSVANSYLRFEEPRSFFAPMSF
                     GNCGYALPTIIGAKCAAPHRPAIAYAGDGAWGMSMVELMTCVRHDIPVTAVVFHNRQW
                     GAEKKNQVDFYNRRFVAGELDNQSFAGIARAMGA"
     gene            1800..2618
                     /locus_tag="VFF72_12860"
     CDS             1800..2618
                     /locus_tag="VFF72_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZV94102.1"
                     /translation="MKIVASSAPALATVDGDTPTLRLFALLEVIASKDQLFSLQALVE
                     ATGLPKPTLHRMLQQLEGANLLQREGDGRHYGTGVRLRRLAESLLMNDSHHGARHAVL
                     RHLVEQIGESCNITTLSGSEVVYLDRVETAAPLRFYLHPGSRVPVHCSASGKVFLAQM
                     TPAQRRRLLAHAPLERCTPNTLTDAARLERELRRVARDGFAIDDEEFLPGLVCLAVAV
                     PARGRPSSLCVALQAPVMRLSTARAKTLRPLLQRAAEALGEIDADSAAAGLARA"
BASE COUNT          382 a          930 c          941 g          462 t
ORIGIN      
        1 tcggcgccca tcgcgcgggc gatgccggcg aagctctggt tgtcgagctc accggcaacg
       61 aagcggcggt tgtagaagtc aacctggttc ttcttctcgg cgccccactg gcggttgtgg
      121 aacaccaccg cggtgaccgg gatgtcgtgg cgcacgcagg tcatcagctc gaccatgctc
      181 atgccccagg cgccatcgcc ggcgtaggcg atggccgggc ggtgcggcgc cgcgcacttg
      241 gcgccgatga tggtcggcag cgcgtagccg cagttgccga agctcatcgg cgcgaagaac
      301 gagcgcggct cctcgaagcg caggtagctg ttggcgaccg agttgatgtt gccgatgtcg
      361 gtcgagacca tgacgtgcgc cggcatcgcc ttctcgagct cgcgcagcac ctggcgcggg
      421 tgcagccagc ggccctcttc cttcttctgc tcctcgatca tgtcgaggct gtacggatcc
      481 ttctcgtggg tccactcggt gagttccttc tcccacgccg ccttctcgtc ggcgaccttc
      541 ttcgcgcgct cggccttgct ggcgtcgcag gcgaggctcc tgccttcgag gcgcttcgag
      601 atcgccgccg cggccgcctt ggcgtcgccg gcgatgccga cggagatctt cttcaccagg
      661 ccgagattgg tgtggtcggc ctccacctgg atgatcttgg cgtccttcgg ccagtagtcg
      721 aggccatgct gcggcagggt gccgaacggg cccatgcgag agccgagggc gacgacgacg
      781 tcggcctggg cgatcagctt catcgccgcc ttcgagccct ggtagccgag cggcccgcac
      841 cagagcgggt ggctggcggg gaaggcgtcg tttctcaggt atccggtgac gaccggcgca
      901 cccagacgct cggcgagcgc tttcgtctcc tcgacggcgt cgcccatgac gacgccgccg
      961 ccggcgagga tgaccgggaa cttcgccttg gcgagcaagt cggcggcctc gttcaggctc
     1021 tgctcgccgc cggcaccgcg ctcgacgcgc atcggcttcg ggatctcgac ggtgatctcg
     1081 ccgtagaagt agtcgcgcgg gatgttgagc tgggtcggcc ccatctcgct gatcgcgcga
     1141 tcgaagcagc gcgccgtgta ctcggccatg cgcctcgggt tgttgacgtg cccctggtac
     1201 ttcgtgaatt cctggaacat cggcagctgg ttcgcctcct ggaagccgcc caggcccatg
     1261 cccatcgtcc cggtctcggg agtgatcatg accaccgggc tgtgcgccca gaacgcggcg
     1321 gcgatcgccg tgacgcaatt gctgatgccg gggccgttct ggccgatgac gacgccgtgc
     1381 cggccgctga cccgggcgta gccgtcggcc atgtgcgcgg cgccctgctc gtggaccacc
     1441 gggatgaggc ggattccggc cggcgcgaag atgtccatcg cgtccatgaa ggccgagccc
     1501 atgatgccga agatgtcggt cacgcggttg gcgaccatcg tttcgacgaa ggcctcggag
     1561 ggcgtcatct tctgcacgcc ctgcacgggc ttgcgggtat cggcggcggg aagggggtct
     1621 cggttcatgt tcgttccttg gcgacggggg agggcggcgg aggcggcgac gacggtcgac
     1681 tccggcgagg acggcgggat gcgcgaatga tagggagacg cgaccgggag gtcaaacgga
     1741 attttgaaaa acggaacatg gtgtacgaaa tatcggaacg cggatatgat gcccggatca
     1801 tgaagatcgt cgcttcgtcc gctcccgcgc tggccaccgt cgacggcgat acgccgaccc
     1861 tgcgcctgtt cgccctcctc gaggtgatcg cctcgaaaga ccagctcttc tcgctgcagg
     1921 ccctggtcga ggcgaccggc ctgcccaagc cgacgctgca ccgcatgctg cagcagctcg
     1981 agggggccaa cctgctgcag cgcgaaggcg acggccgcca ctacggcacc ggcgttcgcc
     2041 tgcgccggct ggccgaaagc ctgttgatga acgacagcca ccacggggcc cgccacgccg
     2101 tgctgcgtca tctcgtcgag cagatcggcg agagctgcaa catcacgacg ctctcgggca
     2161 gcgaggtggt ctacctcgat cgcgtcgaga ccgcggcgcc gcttcgcttc tacctgcatc
     2221 cgggatcccg ggtgccggtg cattgctcgg ccagcggcaa ggtcttcctc gcccagatga
     2281 cgccggcgca acgacggcga ctgctcgccc acgcgccgct cgagcgctgc acgcccaaca
     2341 ccctgaccga cgcggcgcgg ctcgagcgcg aattgcgccg cgtcgcgcgc gacggcttcg
     2401 cgatcgacga cgaggaattc ctgcccgggc tcgtctgtct tgccgtcgcg gtgccggccc
     2461 gcggtcggcc gtccagcctg tgcgtggcct tgcaggcgcc cgtcatgcgg ctgtcgacgg
     2521 cccgggcgaa gacgctgcgt ccgctgctgc agcgagccgc cgaggcgctc ggcgagatcg
     2581 acgccgactc cgccgcggcc ggcctggccc gggcatgagt tcgccttccg ccagggagcc
     2641 ggcgatgcgc cgcgtcggcg tccgcgaaac cttcggcatc gactccgacc tggtcgttcc
     2701 cgccttcgcc gggcg
//
LOCUS       DASRWS010000672         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_847672, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000672 DASRWS010000000
VERSION     DASRWS010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847672"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFF72_12865"
     CDS             <1..469
                     /locus_tag="VFF72_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZV94103.1"
                     /translation="AKDTRLPAQVGETFASRLRLGTDEIPAFATSVRDFNPLHHDLAA
                     ARAAGYPSLIASGTQVGSLLMALTATHFARPLADGTPRNGLGLGFDIRFRAVVFADED
                     IDLRWRVTSVARKDRLAGWITTLEGEARSPRGVLLSANGALLLRLGAFSAGIG"
     gene            483..1613
                     /locus_tag="VFF72_12870"
     CDS             483..1613
                     /locus_tag="VFF72_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV94104.1"
                     /translation="MLLDDDHRAVQDAVRAFVQDRIAPNAARWDREAHFPAEELRGLA
                     ALGCYGVAVPAELGGAGLDYLALAVILEEIAAGDGATSTIVSVNNCPVCSILMAWADE
                     AQKARWLAPLARGEMLGAFCLTEPHVGSEAGGLKTTARRDGNEYVLDGVKQFITSGRN
                     ADVAIVMAVTDKSAGKKGISAFVLPTGTPGYVVARLEDKLGQHASDTAQIVFEGCRVP
                     ASHRLGDEGQGLRIALSGLEGGRIGIGAQAIGMARAAFEAALAYAKERVAFGKPIVEH
                     QAVQFRLAAMATQIEAARQLVHHAASLKDAGRPCLTEAAMGKLFASEMAERVCSDAIQ
                     VFGGYGYVADFPVERIYRDVRVCQIYEGTSDVQKMLISRSFG"
     gene            1701..>2160
                     /locus_tag="VFF72_12875"
     CDS             1701..>2160
                     /locus_tag="VFF72_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZV94105.1"
                     /translation="MSGAAIDFYFDFSSPYSYVASEWIGPLASKHGREVRWHAILLGV
                     TFQAAELKSPVSHPIKREYSLHDFARSARFAGVPYAQPARFPIATQNAARVFWWLAGS
                     DAARATQWAHDAFRAYFTRGVDLSDPAALKALAAASGLDVAQAETVWNDPH"
BASE COUNT          320 a          745 c          762 g          333 t
ORIGIN      
        1 cgcgaaggac acgcggctgc cggcgcaggt cggcgaaacc ttcgccagcc ggctgcgcct
       61 gggcaccgac gagatcccgg cattcgcgac gtcggtgcgc gatttcaatc ccctgcacca
      121 tgatctcgcc gccgcgcggg cggccggcta cccgtcgctg atcgccagcg gcacgcaggt
      181 gggcagcctg ctgatggcgt tgaccgcgac ccatttcgcc aggccgctcg ccgacggcac
      241 gccgcgcaac ggcctgggcc tcggcttcga catccgcttt cgcgccgtcg tcttcgccga
      301 tgaggacatc gacctgcgct ggcgggtgac gagcgtcgcc cgcaaggatc gcctggccgg
      361 ctggatcacg acgctcgaag gcgaagcccg ctcgccgcgt ggcgtgctgc tgagcgccaa
      421 cggcgcgctg ctgcttcggc tgggcgcgtt ttcggccggc atcggctgaa cggaagggcg
      481 acatgcttct cgacgacgat caccgggccg tgcaggacgc ggtgcgcgcg ttcgtgcagg
      541 accgcatcgc gccgaacgcc gcgcgctggg acagggaggc gcatttcccg gcagaagaat
      601 tgcgaggcct ggccgcgctc ggctgctacg gcgtcgccgt gccggccgag ctcggcggcg
      661 ccgggctcga ctatctcgcg ctggccgtga tcctcgagga gatcgccgcc ggcgacggcg
      721 cgacgtcgac gatcgtcagc gtcaacaact gccctgtctg ctcgattctc atggcctggg
      781 ccgacgaagc gcagaaggcg cgctggctcg caccgctggc gcggggcgag atgctcggcg
      841 ccttctgcct gaccgagccg cacgtgggct cggaagcggg cggcctgaag acgacggcgc
      901 gacgcgacgg caacgagtac gtgctcgacg gcgtcaagca gttcatcacc agcggcagga
      961 acgccgacgt cgccatcgtc atggctgtca ccgacaagtc ggcgggcaag aagggcatca
     1021 gcgccttcgt cctgccgacc ggcacgccgg gctacgtcgt cgcgcggctc gaagacaagc
     1081 tcggccagca cgccagcgat acggcgcaga tcgtcttcga gggctgtcgg gttcccgcga
     1141 gccatcgcct cggcgacgaa gggcaggggc tcaggatcgc cctctcgggt ctcgagggtg
     1201 gccgcatcgg catcggcgcg caggcgatcg gcatggcgcg cgcggcgttc gaggcggcgc
     1261 ttgcctatgc gaaggagcga gtcgcgttcg gcaagcccat cgtcgagcac caggcggtgc
     1321 agttccggct cgcggcgatg gcgacgcaga tcgaggcggc gcgccagctc gtccatcacg
     1381 ccgcgtcgct gaaagacgcc gggcggcctt gcctcaccga ggcggcgatg ggcaagctct
     1441 tcgccagcga gatggccgag cgcgtctgca gcgacgccat ccaggtcttc ggcggctacg
     1501 gctacgtcgc cgatttcccc gtggaaagga tctatcgtga cgtccgtgtc tgccagatct
     1561 acgaaggcac gtccgacgtg cagaagatgc tcatctcccg ctcgttcgga tagcgggcaa
     1621 ggccggcggt tggccggctg gagcgacgaa gacccgggcg cgcggtttcg accgcgttgc
     1681 cggcaaggaa aggacctgac atgagcggtg ccgccatcga tttctacttc gacttctcgt
     1741 cgccgtattc gtacgtcgcc tccgaatgga tcggtccgct cgcgtcgaag cacggccgcg
     1801 aggttcgctg gcacgccatc ctcctgggcg tgacgttcca ggcggccgag ctgaaaagtc
     1861 ccgtcagcca tccgatcaag cgcgagtact cgctgcacga tttcgcgcgc tcggcgcgct
     1921 tcgccggcgt gccttacgcg cagccggcgc ggtttccgat cgcgacgcag aacgcggcgc
     1981 gcgtcttctg gtggctcgcc ggaagcgacg cggcgcgcgc cacccaatgg gcccacgacg
     2041 cgtttcgcgc ctacttcacg cggggtgtcg atctttccga tccggcggcg ctgaaggcgc
     2101 tggccgctgc gtccggcctc gatgtggcgc aggccgagac ggtctggaac gacccgcact
//
LOCUS       DASRWS010000673         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4286725, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000673 DASRWS010000000
VERSION     DASRWS010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286725"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..1015
                     /locus_tag="VFF72_12880"
     CDS             470..1015
                     /locus_tag="VFF72_12880"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HZV94106.1"
                     /translation="MIVGALGFGVAGAKECNGIDFPEQMPADGAALTLNGLGLRQATM
                     FKVNVYVAALYVPRKSSDPGTILDNAPAELILHFVRDVGAGDIAKSWNEGFEKNAKAD
                     LHVLKDRIAMLDGWMTDVKSGQRMIFSFKPGTGVQVSVNGVAKGTIKGDDFAKALLSI
                     WLGPEPPNPEIKAGLLGGACG"
     gene            1026..>2349
                     /locus_tag="VFF72_12885"
     CDS             1026..>2349
                     /locus_tag="VFF72_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZV94107.1"
                     /translation="MTVRDMYRRLLSRTAVFALAAACVLSGCATRPVSGRIEQFVPPT
                     SYAYERPSDNPEDRQNLVLLAFSGGGTRAAAFSFGVLETLRDMEVTTRSGRKVRALDA
                     VDVITGISGGSFTALAYGLYGDKIFEEYEQRFLKRNVQGELIARAFNPFNWGALVSTG
                     WGRSELAANLYDEILFNGATYADLRRDGPQIFVGATDLAEGTRIIFNKANFDVMCSDL
                     SGFRLSRAAAASSAVPVLLSSVTIDNYGGTCGYRFPSWTKLFLDNPDPPRPAARTLRR
                     LQELEAYADRTKRPYLHLVDGGVSDNIGVRGLLDVLATFEALHAAGMATPLDHVRNII
                     VFVVNSLATPPNDWDLHENPPGTLDVLIKATGTPIDRYSYDTVETLKDMQAQWQALRE
                     IREALKGLSRPDPKLDFVMRAPDENIYVIDVSFSVLKDAAERDYLNRLP"
BASE COUNT          418 a          769 c          740 g          422 t
ORIGIN      
        1 gacgccgcgc ccgcagccac cacggcgccg gccgccagag ccagcaaaag cgcgacgacg
       61 gcggcgtacg ccgtccgcac tgtcgagcgc aatctgtcgt ccaatcgcac gtcgtctccc
      121 gcgtggcccg cttggcttca ccacatcgtt ccggggcgag agcgcaaggg taacgccatt
      181 tgggggccgg tgcagtggcc ggcttctccg cagcggagcg aagcgtcgcg cgtgtcgccg
      241 atgcgtcggt ctcgcaggtg tccagaatgg agaggacgct ttgcgtacct tccgctaaga
      301 ggacattggc gcgcgcgagc tcgcgacacc gcttttgtga ccgcaaccat tcgagctgca
      361 tgcagccatg tcgctgggac atcatgaaga acagattccc gctcactgca gcagcgacgc
      421 ccgcgctcgc cggccgcgcg tcgcagcact gcgaatactc gcgacagccg tgatcgtcgg
      481 cgcgcttggt ttcggcgttg caggggcgaa ggaatgcaat ggcatcgact ttcccgagca
      541 gatgccggcc gacggtgccg cactgacgct caacgggctc ggccttcgcc aggcgacgat
      601 gttcaaggtg aacgtctacg tcgcggcgct ttacgtaccg cgcaagtcga gcgacccggg
      661 cacgattctc gacaatgcac cggccgaatt gatcctccat ttcgtccgcg acgtgggagc
      721 gggcgacatc gccaaaagct ggaacgaagg cttcgagaag aatgcgaagg ccgacctgca
      781 tgtgctgaag gatcgcatcg cgatgctcga cggctggatg acggacgtga agagcggtca
      841 gcgaatgatc ttttcgttca agcccggcac gggcgtgcag gtcagcgtca acggcgttgc
      901 gaagggcacg atcaaagggg acgacttcgc caaggccctg ctgtcgatct ggcttggtcc
      961 cgagccgccg aatccggaga tcaaggccgg cctgctcggc ggcgcgtgcg gctgaggctt
     1021 tcccgatgac cgtgcgcgac atgtacaggc gccttctgtc ccgcacggcg gtcttcgcat
     1081 tggcggccgc ctgcgtgctt tccggttgcg ccacgcgccc ggtcagcggg cggatcgagc
     1141 aattcgttcc cccgacgagt tatgcgtacg agcggccgtc cgacaacccc gaggatcgcc
     1201 agaatctcgt tctgcttgcg ttctccggtg gaggcacgcg cgccgcagcg ttctccttcg
     1261 gcgtgctgga aacgcttcgg gacatggagg tcaccacgcg cagcgggcgc aaagttcgcg
     1321 cgctcgacgc agtcgatgtg atcaccggga tctcgggcgg gagcttcacc gcgcttgcct
     1381 atggcttgta cggggacaag atcttcgaag aatacgagca gcgctttctc aagcgcaacg
     1441 tgcagggcga gctgatcgcg cgggcgttca atccattcaa ctggggtgcg ctcgtttcga
     1501 ccggctgggg ccgctccgag ctcgccgcta atctttacga cgagatcctt ttcaacggag
     1561 cgacctatgc ggacctcagg cgcgacgggc cgcagatctt cgtcggcgca accgacctcg
     1621 ccgaaggaac gcgcatcatt ttcaacaagg cgaacttcga cgtcatgtgc tccgatctga
     1681 gcggcttccg cctctcgcgc gccgccgctg cctcgtcggc ggtgcccgtg cttctttcct
     1741 ccgtcaccat cgacaactac ggcggcacgt gcggttaccg ctttccatcg tggacgaagc
     1801 tgtttctcga caaccccgat ccgccgcggc cggctgcccg cacgctgcgg cgcctgcaag
     1861 agctggaggc ctatgccgac cggacgaagc gaccgtatct ccacctcgtc gatggcggcg
     1921 tttccgacaa catcggcgtg cgcggtcttc tggatgtcct cgccacgttc gaggcgcttc
     1981 acgctgccgg aatggcgacg cccctcgacc atgtaaggaa catcatcgtg ttcgtcgtca
     2041 attcgctggc gacacctccg aacgattggg acctgcacga gaatccgccc ggcacgctcg
     2101 acgttctgat caaggcgacc ggcacgccga tcgatcgcta ttcgtacgac accgtcgaaa
     2161 cgttgaagga catgcaggcg caatggcagg cgctgcggga aatccgcgaa gcgctcaagg
     2221 gattgtctcg acccgacccc aagctcgact tcgtcatgcg ggcacccgac gagaatatct
     2281 acgtgatcga cgtatcgttc tcggtcctga aggacgcggc cgagcgcgat tacctgaacc
     2341 gcctgccaa
//
LOCUS       DASRWS010000674         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2871727, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000674 DASRWS010000000
VERSION     DASRWS010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871727"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFF72_12890"
     CDS             <1..476
                     /locus_tag="VFF72_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HZV94108.1"
                     /translation="DPLADVVDADLASRIVWFDALVTNLDRTARNTNMLMWHRRLWLI
                     DHGAALYFHHAGLGDSAAARKPFPLIRDHVLLPWASRVAEVDAELSARLPPRVVEDIL
                     ALVPDAWLADAAPAPAGEPASPAARRAAYVDHFARRLAAPRPFVTEAASARAADV"
     gene            454..864
                     /locus_tag="VFF72_12895"
     CDS             454..864
                     /locus_tag="VFF72_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HZV94109.1"
                     /translation="MPALQTYDYAIVRVVPRVERGEFVNVGVIVSCDATDLLAARIVA
                     DEERIRSIDPAADLVAIGAALGAIEAVCSGAPDTGALARMTPRERFHWLVAPRSAVIQ
                     TSPVHTGRTADCGAALDHLFDRMVGTRAAPGGRN"
     gene            complement(929..1786)
                     /locus_tag="VFF72_12900"
     CDS             complement(929..1786)
                     /locus_tag="VFF72_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94110.1"
                     /translation="MPSRLHRKIRPLSYTLLVFCHLLAASIALGAIVATDLRLLSKLA
                     QDKVRIAPPNEFVARIVMLSLCALWITGGLIVWHGLGQRADYLANPKLQLKIYLVAAL
                     TVNAFVLHRVTFPRLARGRRVPRWTLSDWLVIAVPVAVSNFLWLFAAFLGIARTWNYV
                     VPLGEILATAAGLFVVAQCGVITILAVAGRRVEPDATSWIDRLARSLARIGRLGTSEA
                     ADSRPASGSSSGAKSSSRRRLPNWHPAATWLPSDASLPPLVKHEKAARPELRLVDNWP
                     APEKRGAMR"
     gene            complement(2004..2729)
                     /locus_tag="VFF72_12905"
     CDS             complement(2004..2729)
                     /locus_tag="VFF72_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZV94111.1"
                     /translation="MAIDPASVPTARVSWRPSYRLIPSRYPTVGLYDAIADPADLEVV
                     FAIESFANPRLRQEAGQLHLVAPGERVSGPGATPVMAAFTHLNPEGSRFSDGSYGVYY
                     AAHSLPTAVAEVGHHRAVFLRRTAEPAIDLDLRLITANVEAELHDLGSTRDEAANGGR
                     FAAALDPFDYAPSQSLGRALRAAESWGLRWPSVRDAGGECVGIFRPRALRHAKAAAHI
                     GLHWDGHAMTHWYEKDKPHPLGR"
     gene            complement(2749..3159)
                     /locus_tag="VFF72_12910"
     CDS             complement(2749..3159)
                     /locus_tag="VFF72_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HZV94112.1"
                     /translation="MSALLKPAAARVDLSTPESAAAALRTFFRLADAWQLGIAEQTTL
                     LGVARTTLYQWRQGKVAPLDRHLLERLSHLLGIYSSLHILFPVAQRADEWVRKPNAAP
                     LFGGRSALERMLAGQVADLFVVRQYLDAQRGGKS"
BASE COUNT          517 a         1152 c         1181 g          468 t
ORIGIN      
        1 tcgacccgct cgccgacgtc gtcgacgccg acctggcatc gcgcatcgtc tggttcgacg
       61 cgctcgtgac caacctcgac cgcaccgcgc gcaacaccaa catgctcatg tggcacagga
      121 ggctctggct gatcgaccac ggcgccgcgc tctatttcca tcacgccggc ctcggcgact
      181 cggccgccgc acgaaagccg tttccgctga tccgcgacca cgtcctgctg ccctgggcca
      241 gccgcgtcgc ggaagtcgac gccgagctct cggcgcggct gccgccgcgt gtcgtcgaag
      301 acatcctggc gctcgttccc gacgcctggc tcgccgacgc ggcgccggcg cctgccggcg
      361 agccggcctc tccggcggcg cgacgcgcgg cctacgtcga tcatttcgcg cgccggctcg
      421 ccgcgcctcg acccttcgtc acggaggccg ccagtgcccg cgctgcagac gtatgactac
      481 gcgatcgtcc gcgtcgtgcc gcgcgtcgag cgcggcgagt tcgtcaatgt cggcgtcatc
      541 gtctcgtgcg acgcgacgga cctgctcgcg gcgaggatcg tcgccgacga agaacggatc
      601 aggtcgatcg accctgccgc cgacctcgtc gccatcggcg ctgcgctcgg cgccatcgaa
      661 gccgtctgct ccggcgcgcc ggacaccggc gccctcgcaa ggatgacccc tcgcgaacgc
      721 ttccattggc tggtcgcgcc gcgcagcgcc gtcatccaga cgtcgccggt gcacaccggg
      781 cgcaccgccg actgtggcgc cgcgctggac cacctcttcg accggatggt cggcacccgt
      841 gccgcgccgg gcggtcgcaa ctgacatcgg cgacgcggcg gatgcgggcg ccgcttctga
      901 ggagcccggt cggtcggatg accccggctc agcgcatcgc gccccttttt tccggcgccg
      961 gccagttgtc gaccagccgc agctccggcc gcgccgcctt ctcgtgcttg acgaggggcg
     1021 gcagcgacgc gtcggacggc agccaggtgg ccgcgggatg ccagttcggc agacggcgac
     1081 gactcgacga cttcgcgccc gatgacgatc ccgaagccgg ccgcgaatcg gccgcctcgg
     1141 aagtgcccag ccggccgatg cgcgccagcg agcgggccag ccggtcgatc cagctcgtcg
     1201 cgtcgggctc gacccggcgt ccggcgacgg cgaggatggt gatcacgccg cattgcgcga
     1261 ccacgaacag gcccgcggcc gtggcgagga tctcgcccag cggcacgacg tagttccacg
     1321 tccgggcgat gccgaggaag gcggcgaaca gccagaggaa gttcgagacg gcgacaggca
     1381 ccgcgatgac gagccagtcg gagagcgtcc agcgcggcac gcgccttccc ctggcgagcc
     1441 gcgggaaggt gacccgatgc agcacgaagg cattcacggt gagcgccgcg acgaggtaga
     1501 tcttcaactg cagcttcgga ttggcgaggt agtcggcacg ctggccgaga ccatgccaga
     1561 cgatcaggcc accggtgatc cacagcgcgc acaacgagag catgacgatg cgcgccacga
     1621 attcgttcgg cggcgcgatc cgcaccttgt cctgggcgag cttggagagc aggcgaaggt
     1681 cggtggcgac gatggcaccg agcgctatcg aggcggcgag gagatggcag aagacgagca
     1741 gcgtgtacga caaggggcgg atctttcgat ggaggcggga aggcatcgct gcgctgcctg
     1801 cgctgcatca acgcacggga cgtgccagcc ccgacccgct cgccgggcgt ggatttgtgg
     1861 gcttcggcga ccccctttcg gacagttctc gcccgtgcgc cctcgctgac cggatcgccc
     1921 ataacccgat gcgccactat tgcaagcaga tgcaggcttt gtaagcaacc gcgtcgcatg
     1981 cgccggcgac cggcaccgac ggatcagcgg ccgaggggat ggggcttgtc cttctcgtac
     2041 cagtgcgtca tggcgtgtcc gtcccagtgc aggccgatgt gcgcggccgc cttggcatgc
     2101 cgcagcgccc ggggccggaa gatgccgacg cattcgccgc ccgcgtcgcg cacgctcggc
     2161 cagcgcaggc cccagctttc ggccgcgcgc aaggcgcggc cgagcgactg cgacggcgcg
     2221 tagtcgaacg ggtcgagcgc ggcggcgaag cggccaccgt tggccgcctc gtcgcgcgtc
     2281 gagccgaggt cgtggagctc ggcctcgacg ttggcggtga tcaggcgcag gtcgagatcg
     2341 atcgccggct cggccgtgcg gcgcaggaag acggcgcggt ggtggcccac ctcggccacc
     2401 gccgtcggca gcgaatgcgc tgcgtagtag acgccgtaac tgccgtcgga aaagcgcgag
     2461 ccttcgggat tgaggtgggt gaaggcggcc atcaccggcg tcgcgcccgg cccgctgacg
     2521 cgctccccgg gcgccaccag atgcagctgg cccgcttcct ggcgcagccg cgggttggcg
     2581 aacgactcga tcgcgaagac gacctcgagg tcggccggat cggcgatcgc gtcgtacagg
     2641 cccacggtcg ggtagcgcga cgggatcagc cggtacgagg gacgccagga aactcgcgcc
     2701 gtcggcacgc tcgccggatc gatggccatg ctgtgcagcc gacgagattc aggacttgcc
     2761 gccgcgctgc gcgtcgaggt actggcggac gacgaagaga tcggccacct ggccagcgag
     2821 catgcgctcg agcgcagagc ggccgccgaa cagcggcgcc gcattgggct tgcgcaccca
     2881 ttcgtcggcg cgttgcgcga cgggaaaaag gatgtgcagc gaggagtaga tgcccaacag
     2941 gtgcgagagc cgttcgagca ggtgccggtc gagcggagcg accttgccct gcctccactg
     3001 gtagagcgtg gtgcgggcga cgccgagcag cgtcgtctgc tcggcgatgc cgagctgcca
     3061 ggcatcggcg aggcgaaaga aggtgcgcag tgccgcggcg gccgattccg gtgtcgacag
     3121 gtcgactctg gcggcagcgg gcttgagcaa ggcactcatg gtgatccgag gttattccat
     3181 gagcggacaa aatgcaatga attgtccgtg agcgaactgt ggaatctcga ggcctgcgcc
     3241 gacatgatcg cgccgctgcc cgccctttcg gccgatcccg cggccatgca tctggtggag
     3301 cgaccggccc tacgacgg
//
LOCUS       DASRWS010000675         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3139060, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000675 DASRWS010000000
VERSION     DASRWS010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139060"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFF72_12915"
     CDS             <1..683
                     /locus_tag="VFF72_12915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV94113.1"
                     /translation="SDLMLLLARTTPRDQTKKRTEGLSVFLVDMRQALGHGLTIRPIR
                     TMMNHNSTEVFFDNMEVPAENLIGEEGQGFRYILGGMNAERILIAAESIGDSRWFIEK
                     ATDYAKERKLFGRPIGQNQGVQFPIARAYAQTEAADLMVRKAATLYEAGLPCGAEANM
                     AKMLASEAAWAAADMCVQTHGGFGFAEEYDIERKFRETRLYMVAPISTNMILNFVSEH
                     VLGLPRSY"
     gene            complement(701..1312)
                     /locus_tag="VFF72_12920"
     CDS             complement(701..1312)
                     /locus_tag="VFF72_12920"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HZV94114.1"
                     /translation="MRERGTLLPVLERLFEGPRESRLTLQAFLAGLGGRSYAFIVAAL
                     DLPNCVPTGIPLLSTVTGVPMLWLVAQAWLGHPTPSLPQFIGLRSLPRGRLQDFLGRA
                     RRRIEWIEAAIHPRRDWWVTGIRRRLLQLTLITMIVVLAVPIPFDNLLPAWSILFFCL
                     ALIEGDGVMAMLGWAFAVLTVLWTTFLLIVGPLVVAGVFKSFF"
     gene            complement(1309..>2114)
                     /locus_tag="VFF72_12925"
     CDS             complement(1309..>2114)
                     /locus_tag="VFF72_12925"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZV94115.1"
                     /translation="SGADGLILDLEDSVAADRKMVARDMALAFLRAARRDTGPKLYIR
                     VNALDTGLTLGDLATIMQGRPDGIVFPKCVGQRDLDLLATYLEAFEVREGIEGGTTRI
                     LTVATESAAAVLALTAATAKHPRLIGHSWGGEDLMADLGALAKGPSPGVYDDTFKFAR
                     TVNLLASVAAGVTAYDTVYPDIKNIEGLRAEAHEARRMGYGGKIAIHPDQVAIIHEVF
                     TPSAQEVDWAKRVVDAFESNPGSGVLTLDGKMLDRPHFVLARRLLARAG"
BASE COUNT          406 a          655 c          720 g          333 t
ORIGIN      
        1 attccgatct gatgctgctg ttggcgcgca ccacgccccg ggaccagacg aagaagcgca
       61 ccgaggggct atcggtgttc ctggtcgata tgcgccaggc gctcggtcat ggtcttacca
      121 tccgaccgat ccgcaccatg atgaatcaca acagcacgga agtgttcttc gacaacatgg
      181 aagtgccggc ggagaatttg atcggcgagg aggggcaggg gttccgctac atcctgggcg
      241 gcatgaacgc cgagcgcatc ctgatcgctg ccgaaagcat cggcgattcc cgctggttca
      301 tcgagaaggc gaccgactac gccaaggaac gcaagctgtt cggccggccg atcggccaga
      361 accagggcgt gcaatttccg atcgcacgcg cttatgcgca gaccgaagcg gcggatctca
      421 tggtgcgcaa ggcggcaacg ctctacgaag ccggtttgcc gtgcggggct gaggcgaaca
      481 tggcgaagat gctcgcctcc gaagcggctt gggccgcggc cgacatgtgc gtgcaaaccc
      541 atggcggctt cggctttgcc gaggaatacg acatcgagcg caagttccgc gagacgcggc
      601 tttacatggt ggcgccgatc tcgaccaaca tgatcctgaa tttcgtgtcc gagcatgtgc
      661 tcggcctgcc gcgatcctac tgacaggcgc gctgcaccgg tcaaaagaaa gacttgaaca
      721 cgccggccac gaccagcgga ccgacgatca acaaaaaggt cgtccacagg acggtcaaga
      781 cggcaaatgc ccaaccgagc atggccatga cgccgtcacc ttctatcagc gccaggcaga
      841 agaacaggat cgaccacgcc ggcaggaggt tgtcgaacgg gatcgggacc gcgagcacca
      901 cgatcatggt gatgagcgtc agctgcagca gccggcgtcg tatccccgtc acccaccagt
      961 cacggcgcgg atggatggcg gcttcgatcc attcgatccg gcgccgagcg cggcccagaa
     1021 agtcctggag cctgcctcgc ggtagcgacc gcaggccgat gaactgtggc aaagacggcg
     1081 tcggatggcc gagccaggcc tgggccacca accagagcat gggcacgccg gtgacggtag
     1141 acagaagcgg aatgccagtg ggcacgcagt tcggcaggtc gagcgccgcg acgatgaagg
     1201 cgtacgaacg gccgccgagg ccggcgagga acgcctggag cgtgagcctc gactcgcgcg
     1261 gcccctcgaa cagtcgctcg aggacgggca gcagcgtgcc gcgttccctc atccagcgcg
     1321 ggcgagcagg cggcgggcga gcacgaaatg gggcctgtcg agcatcttgc cgtcgagcgt
     1381 cagcacgccg gagccggggt tgctttcgaa ggcgtcgacc acccgcttgg cccagtcgac
     1441 ttcctgcgcc gacggggtga agacctcgtg aatgatcgcg acctggtcgg gatggatcgc
     1501 gatcttgccg ccatagccca tgcggcgcgc ctcgtgcgcc tccgcgcgca ggccctcgat
     1561 gttcttgatg tcgggataga ccgtgtcata ggcggtgacg ccggctgcga ccgacgccag
     1621 cagattgacg gtgcgtgcga acttgaaggt gtcgtcgtag acgccgggcg acggtccttt
     1681 ggcgagcgcc ccgaggtcgg ccatgagatc ctcgccgccc caggaatgcc cgatcaggcg
     1741 cggatgcttg gccgtcgctg cggtcagggc cagcacggcc gcggcacttt cggtcgcgac
     1801 ggtaaggatg cgggtcgtgc cgccctcgat tccctcccgc acctcgaagg cttcgagata
     1861 ggtcgcaagc aggtcgaggt cgcgctggcc gacgcatttg ggaaagacga tgccgtcggg
     1921 cctaccttgc atgatggtcg cgagatcgcc gagcgtgagt ccggtatcga gcgcattcac
     1981 ccggatgtag agtttggggc cggtgtcgcg cctggcagcg cgcagaaagg ccagcgccat
     2041 gtcgcgtgcg accatcttgc ggtcggcggc gacggaatct tcgagatcga ggataaggcc
     2101 gtcggcgcca gatt
//
LOCUS       DASRWS010000676         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4004730, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000676 DASRWS010000000
VERSION     DASRWS010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4004730"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFF72_12930"
     CDS             complement(<1..1116)
                     /locus_tag="VFF72_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZV94116.1"
                     /translation="MDAYWLDWANLLLRWAHVITGIAWIGASFYFVGLDNSLTPPADP
                     TERDKGVGGELWAVHGGGFYHQQKYPVAPARLPAHLHWSMWESYSTWLTGFALFAVLY
                     LFNANTFLIDRSVHEWSSAAAVTTALGFFVAFWLVYDGICRAFGRGRHGDIVVGGLVC
                     AFIVFAAWLSCHLFAGRAAFLLVGAMMATTMSANVLVWIIPGQRKVVAALREGRPVDP
                     VHGQRGKQRSVHNTYFTLPVVFTMISNHYGFLFEGRYNWLVLVVLMLAGALVRVSFVL
                     RHKALAEARPVPWGWAIAGTLVVCATVAWQMPAPSAAPLAGLPPPTFAQVRAVIAERC
                     QMCHNAAYNNKGVRLDTPELIAAHAQQIDQQAVVLRLM"
     gene            complement(1138..1500)
                     /gene="uraH"
                     /locus_tag="VFF72_12935"
     CDS             complement(1138..1500)
                     /gene="uraH"
                     /locus_tag="VFF72_12935"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HZV94117.1"
                     /translation="MGKLTTHVLDTAHGCPAAGMRVELRRVTGEQRSDDAPVVALALD
                     ADGRAGAPLLQGEAMSAGRYRLTFGVARYFRGRGVVLPEPPFLGEVSIDFGIADVSGH
                     YHVPLLVSPWAYSTYRGS"
     gene            1578..2348
                     /locus_tag="VFF72_12940"
     CDS             1578..2348
                     /locus_tag="VFF72_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZV94118.1"
                     /translation="MPATAPLRAIRAAASAASPRRGRSESSTDDATRRIADAIVAAIV
                     ERRLMPGTKLAEQKIADIFRVSRTLVRQALNQLSRDQLVTLERGRGARVARPSVDEAR
                     QVFEVRRVLEAAMIRRAAHELTGPQVKELRAHLKAEQAAVGRLDISGRTRLLADFHVV
                     IAGMLGNQVLAAMLSELVSRSSLIALMYQSADSAGHSLDEHVAIVDAVEQRDARTAAR
                     LMAAHLAHVEEDLRLDFRSTGPTAARPTTALQPAAAFA"
     gene            2345..>3061
                     /locus_tag="VFF72_12945"
     CDS             2345..>3061
                     /locus_tag="VFF72_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZV94119.1"
                     /translation="MTEKTPRRDSRGHVRSATSSGGARSAYPRDLKGYGADVPHARWP
                     DGARIALQFVLNYEEGGENNVLHGDAASETFLSELGVAPAFAARHMSIESLYEYGSRA
                     GVWRILREFDARGLPLTVFAVGMALERYPEMATEFMARGHEIASHGLRWIHYQQLAEA
                     TERSHVEAATRSLRALTGGQWPLGWYTGRDSPNTRRLVVEHGGYEYDSDYYGDDLPCW
                     IEVETADGKRTAQLVVPYTLD"
BASE COUNT          514 a         1094 c         1048 g          405 t
ORIGIN      
        1 catcagcctg agcaccaccg cctgctggtc gatctgctgc gcgtgggcgg cgatcaactc
       61 gggcgtgtcg aggcgcacgc ccttgttgtt gtaggcggcg ttgtggcaca tctggcagcg
      121 ctcggcgatg acggccctca cctgcgcgaa agtcggcggc ggcagcccgg ccagcggcgc
      181 ggcggacggc gccggcatct gccaggccac cgtggcgcag acgacgagcg tcccggcgat
      241 cgcccagccc cacggcaccg gacgggcctc ggcgagcgcc ttgtggcgaa gcacgaacga
      301 gacccggacc agcgcgccgg caagcatcag caccaccagc acgagccagt tgtatcggcc
      361 ttcgaagaga aagccgtagt ggttgctgat catcgtgaag acgaccggca gcgtgaagta
      421 ggtgttgtgc acgctgcgct gcttgccgcg ctggccgtgc accggatcga cggggcgacc
      481 ttcgcgcaag gccgccacga ccttgcgctg gccgggaatg atccagacca gcacgttggc
      541 gctcatcgtc gtcgccatca tcgcgccgac gagcagaaag gcggcgcgcc cggcgaacag
      601 atgacacgac agccacgccg cgaagacgat gaaggcgcag accaggccgc cgacgacgat
      661 gtcgccgtgg cggccacggc cgaaggcgcg gcagatgccg tcgtagacca gccagaaggc
      721 gacgaagaag ccgagcgcgg tcgtcaccgc cgcggcgctc gaccattcgt gcaccgagcg
      781 gtcgatgagg aaggtgttgg cgttgaagag gtagagcacc gcgaacagcg cgaagccggt
      841 cagccacgtc gagtagctct cccacatcga ccagtgcagg tgggcaggca gacgggccgg
      901 cgcgaccggg tacttctgct ggtgatagaa gccgccgccg tgcaccgccc acagctcgcc
      961 gccgacgccc ttgtcgcgct cggtcgggtc ggccggcggc gtcaggctgt tgtcgaggcc
     1021 gacgaaatag aacgaggcgc cgatccaggc gatgccggtg atgacatgcg cccagcgcag
     1081 cagcaggttc gcccagtcga gccagtaggc atccatcgtg tgtgttcgag gcgcgaatca
     1141 gctgccgcgg taagtggagt aggcccacgg gctgacgagc aggggcacgt ggtagtgacc
     1201 ggaaacgtcg gcgatgccga agtcgatcga cacctcgccc aggaacggcg gctcgggaag
     1261 gacgacgccg cggccgcgga agtatcgggc gacaccgaag gtcaaccggt agcggcccgc
     1321 cgacatcgct tcaccctgca gcagcggcgc gccggcgcga ccgtcggcgt cgagcgccag
     1381 cgcgacgacg ggcgcgtcgt ccgatcgttg ctcgcccgtc acccggcgca gctcgacgcg
     1441 catgccggcg gcgggacagc cgtgcgcggt atcgagaacg tgcgtcgtca gttttcccat
     1501 gcgcttcccc acgacgtccc ggatgcttgc ggtccgtgca aaagtgtata cactttgccg
     1561 gccaacgcaa gccagcgatg cccgcgaccg ccccgcttcg agccatccgc gcggccgcgt
     1621 cggccgcctc gccgcgccgc ggtcgcagcg agtcgtcgac cgacgacgcg acccgacgga
     1681 tcgccgacgc gatcgtcgcc gccatcgtcg agcgccgcct catgcccggc accaagctcg
     1741 ccgagcagaa gatcgccgac atcttcaggg tctcgcgaac gctcgtccgc caggccctca
     1801 accagttgag ccgcgaccag ctcgtcaccc tcgagcgcgg ccgcggcgcc cgcgtcgccc
     1861 ggccgagcgt cgacgaagcg cgccaggtct tcgaggtgcg ccgggtgctc gaagcagcga
     1921 tgatccggcg cgccgcgcac gagctcacgg ggccgcaggt gaaggaattg cgcgcccatc
     1981 tgaaggccga gcaggcagcg gttggccggc tcgacatttc ggggcggacg cggctgctgg
     2041 ccgacttcca cgtcgtcatc gccggcatgc tcggcaacca ggtcctcgcc gccatgctct
     2101 cggaactcgt ctctcgctcg tcgctgatcg cgttgatgta ccagtcggcc gattcggcgg
     2161 ggcattcgct cgacgagcac gtcgccatcg tcgatgccgt cgagcagcgc gacgcacgga
     2221 ccgcggcacg cctcatggcg gcgcacctgg cgcacgtcga ggaggatctg cgcctcgatt
     2281 tccgctcaac cggcccgacg gcggcgcgac cgacgacggc gctgcaaccg gcggccgcct
     2341 tcgcatgacc gagaagacgc cacgacgcga ctcgcgcggc cacgtccgtt cggcaacgtc
     2401 gagcggcggg gcacgctccg cctacccgcg cgatctcaag ggctacggtg ccgacgtgcc
     2461 gcacgcccgc tggcccgacg gcgcccgcat cgccctgcaa ttcgtcctca actacgaaga
     2521 aggcggcgag aacaacgtac tgcatggcga cgccgcctcc gagaccttcc tctcggagct
     2581 cggcgtcgcg ccggccttcg cggcacggca catgtcgatc gaatcgctct acgaatacgg
     2641 ctcgcgcgcc ggcgtctggc gaatcctgcg cgagttcgac gcgcgcggcc tgccgctcac
     2701 cgtcttcgcc gtcggcatgg cgctcgagcg ctatcccgag atggccacgg aattcatggc
     2761 gcgcggccac gagatcgcca gccacggcct gcgctggatc cactaccagc agctcgccga
     2821 ggccaccgag cgcagccacg tcgaggcggc gacccggagc ctgcgcgccc tcaccggcgg
     2881 ccagtggccg ctcggctggt acaccggccg ggacagcccg aacacgcgac gcctcgtcgt
     2941 cgagcacggc ggctacgaat acgacagcga ctactacgga gacgacctgc cgtgctggat
     3001 cgaggtggag acggccgatg gcaagcgcac ggcccagctc gtcgtgccgt acaccctcga
     3061 t
//
LOCUS       DASRWS010000677         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4289578, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000677 DASRWS010000000
VERSION     DASRWS010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4289578"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1382
                     /locus_tag="VFF72_12950"
     CDS             90..1382
                     /locus_tag="VFF72_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV94120.1"
                     /translation="MKKRTFLLAACAACGVATAFPAAAQKVTEINFYYPVAVGGPVTK
                     TIDKMAADFGKENPDIKVNPVYSGTYQESIVKALTATKSGKPPQLAVLLSTDMFSLID
                     EDAIVPIDSIARSADDHKWLDGFYKSFMLNSRTGGHTWGVPFQRSTIVLYWNKDAFKE
                     AGLDPEKGPATWQQMVDDAKKLTKRDASGNVSQWGVEIPSSGFPYWLFQGLTTPNGAI
                     LMNEAGTETYFDKPAVIEALQFWVDLSTRYKVMPGGVIEWGTTPKDFFEKKAAMIWTT
                     TGNLTNIRKNAGFPFGVAMLPKEKRFGSPTGGGNFYIFAKTSPAEQAASLRFIKWATA
                     PQRAAEWSIATGYVAVTPAAWDTPEMKQYAQEVPQALVARDQLQYSVAELSTHDNQRV
                     TKALNDALQAALTGSKKPAEALKDAQREATRILKDYRK"
     gene            1473..>2102
                     /locus_tag="VFF72_12955"
     CDS             1473..>2102
                     /locus_tag="VFF72_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZV94121.1"
                     /translation="MVLLVAFTHWPALATVWDSFMSTGRRGRPSHFIGIENYRSMVQD
                     DVFWQAFVNNVVYAIGTVPLSIALAIAMALWVDSRIRGRGFLRLAYFTPTVLPMIAVA
                     NIWLFFYTPQYGLLEQITHALHLPSHNWLGDKDTALGALIVVAIWKEAGFFMIFYLAA
                     LQSIPPTLAEAADIEGASRAYYLRRVVLPLLMPTTLFVAVNALINAFRLV"
BASE COUNT          379 a          721 c          675 g          327 t
ORIGIN      
        1 cgaggacagc cacaccttcg acgccgccag cggacggcgc cgcgacgaca tcgttcccgt
       61 ttccacctga ccgatccgag gagaccgaca tgaagaagag aacgttcctg ctcgccgcct
      121 gcgccgcttg cggcgtcgcc acggcctttc ccgccgcggc gcagaaggtg accgagatca
      181 acttctacta ccccgtcgcc gtcggcggcc cggtgacgaa gacgatcgac aagatggcgg
      241 ccgatttcgg caaggaaaac cccgacatca aggtcaaccc ggtctactcg ggcacctacc
      301 aggagagcat cgtcaaggcg ctgacggcga ccaagtcagg caagccgccg cagctcgcgg
      361 tgctgctctc gaccgacatg ttctcgctga tcgacgagga cgcgatcgtg ccgatcgact
      421 cgatcgccag gagcgccgac gaccacaagt ggctcgacgg cttctacaag agcttcatgc
      481 tgaacagccg caccggaggg cacacctggg gcgtgccttt ccagcgctcg acgatcgtcc
      541 tctactggaa caaggacgcg ttcaaggagg ctgggctcga tcccgagaaa ggcccggcga
      601 cctggcagca gatggtcgac gacgcgaaga agctcaccaa gcgcgacgcg tcgggcaacg
      661 tcagccaatg gggcgtcgag atcccgtcgt cgggctttcc gtactggctg ttccagggcc
      721 tgacgacgcc caacggcgcg atcctcatga acgaggccgg caccgagacg tatttcgaca
      781 agccggcggt gatcgaggcg ctgcagttct gggtcgacct gtcgaccagg tacaaggtca
      841 tgcccggtgg cgtcatcgag tggggcacga cgccgaagga tttcttcgag aagaaggcgg
      901 cgatgatctg gacgaccacc ggcaacctca ccaacatccg caagaacgcc ggctttccgt
      961 tcggcgtggc gatgctgccc aaggagaagc gcttcggcag cccgaccggc ggcggcaatt
     1021 tctacatctt cgcgaagacc tcgccggccg agcaggcggc gtcgctgcgc ttcatcaagt
     1081 gggcgacggc gccgcagcgc gccgccgagt ggagcatcgc caccggctat gtcgccgtga
     1141 cgccggcggc ctgggacacg cccgagatga agcaatacgc gcaggaggtg ccgcaggcgc
     1201 tggtcgcgcg cgaccagctc cagtactcgg tcgccgagct gtcgacgcat gacaaccagc
     1261 gcgtcaccaa ggcgctcaac gacgccctgc aggccgccct cacgggctcc aagaagcccg
     1321 cggaggcgct gaaggatgcg cagcgggaag cgacgcgaat cctgaaggac tacaggaagt
     1381 agcagccgcc ggttcggggg cggggatcgc gaggagccga actggagaca acgcgcgctc
     1441 gcgtcacgcc ctggctgctc ctgacgccgg cgatggtgct gctcgtcgcc ttcacgcact
     1501 ggccggcgct ggccaccgtg tgggacagct tcatgtcgac gggtcgccgc ggccggccgt
     1561 cgcacttcat cggcatcgag aactaccgct cgatggtcca ggacgacgtg ttctggcagg
     1621 cgttcgtgaa caacgtcgtc tacgcgatcg gcacggtgcc gctgtcgatc gccctggcga
     1681 tcgccatggc gctgtgggtc gattcgcgga tccgcggccg cggcttcctg cgcctggcct
     1741 acttcacgcc gacggtgctg ccgatgatcg ccgtcgccaa catctggctc ttcttctaca
     1801 cgccgcaata cggcctgctc gagcagatca cccacgcgct gcacctgccg agccacaact
     1861 ggctcggcga caaggacacc gcgctcggcg cgctcatcgt cgtcgccatc tggaaggagg
     1921 cgggcttctt catgatcttc tacctcgcgg cgctgcagtc gatcccgccc acgctcgccg
     1981 aggccgcgga catcgagggg gcctcgcgag cgtattacct gcggcgcgtg gtgcttccgc
     2041 tcctcatgcc gaccaccctc ttcgtcgcgg tgaacgcgct catcaacgcg tttcgcctcg
     2101 tc
//
LOCUS       DASRWS010000678         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2295940, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000678 DASRWS010000000
VERSION     DASRWS010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295940"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="VFF72_12960"
     CDS             complement(<1..1614)
                     /locus_tag="VFF72_12960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HZV94122.1"
                     /translation="MTPDLFDSPPAAADGDAVALGEYAERAYLEYALSVVKGRALPDV
                     SDGQKPVQRRILYAMARMGLAFTTGAGPKAVKSARVVGDVLGRFHPHSDQAAYDALVR
                     MAQDFSQRYPLVDGQGNFGSRDGDGAAAMRYTEARLAPISRLLLDELDDGTVDFIANY
                     DGSTEEPRLLPARLPFVLLNGASGIAVGLATEIPSHNLREVAAAAVALLRDEKLGDDE
                     LGRLLPGPDFPGGGQIISADDEIRAAYASGRGTLKVRARWKIEDLARGQWQVVVTELP
                     PGTSAQKVLEEIEELTNPKLRAGKKTLGAEQLQLRATVLAVLDAVRDESSRDAPVRLV
                     FEPKTSRIEQSELLTTLLAHTSLESSTPINLTMVGADGRPTQKSLRRMLVEWNGFRLL
                     TVERRSRHRLGRVAERIHVLEGRHLVLLHIDEVIAIIRAADEPKAALIARFALSERQA
                     DDILEIRLRQLARLEAIRIEQELKDLRGEEARLGELLASPSALKRTVIREIEADAKAF
                     GDDRRTLIEVQKRAVAELRVVDEPVTVVVS"
     gene            complement(1802..2905)
                     /locus_tag="VFF72_12965"
     CDS             complement(1802..2905)
                     /locus_tag="VFF72_12965"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZV94123.1"
                     /translation="MSADSDLVPTRQELARALMGYAVPDTRIGVRLFLVDFVVYAAGL
                     SLALFASQPAWRVLGAVLVGLKMGSLYTLAHDAVHNSLTASSRLNKVLSAIGYLVSLH
                     NYRIRWYDHLVIGHHPGLNGPQPDVYRPLSWQEYCNAPAWRRLWERFIRAPHVLAFAP
                     YGILSRWLRAEVLPNKAMTPKFRAQAWAFGALLVAYLCAIVACLARRNGGDAIGLLAD
                     AVLVIGLPFFIMQTLQAAILYFQHTHPQIPWFGPGDARMEDFGPEALTVHVRIPAFLS
                     SVTHDICEHPAHHVVPAIPCYRLRAAQARLNELLGDRALAVPLSPRVLGDIMRRCKVY
                     DYEHHVWLDFKGQPTTGRTVPDPPLPAGLVAAA"
     gene            complement(2948..>3896)
                     /locus_tag="VFF72_12970"
     CDS             complement(2948..>3896)
                     /locus_tag="VFF72_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZV94124.1"
                     /translation="MFHANSWGLPYAAVMLGVKLVFPGPHLHPDDLLDLMQIEPPTLS
                     LGVPTIWMGLIQAYERALVEQPGRWTLPAGMRSLVGGAAVPESLIRAFARHDVSIVQG
                     WGMTETSPLASVSYPRVELRGKGEDEQFRRAAMAGVPVPLVDIRIRADDGHDAPWNGT
                     AMGEIQCRGPFITGSYHEVPVTDEKFTDDGWLRTGDVASMDELGFIRITDRTKDLIKS
                     GGEWISSVDLENALMGHPSIAEAAVIAIPDPKWSERPLACVVPRPGQAVDPAALDAHL
                     LAHGFARWQLPDRYESIDAVPRTSTGKFWKLKLRERFPA"
BASE COUNT          628 a         1307 c         1375 g          586 t
ORIGIN      
        1 gctgaccacg accgtcaccg gctcgtcgac gacgcgcagc tcggcaacgg cgcgcttctg
       61 cacttcgatg agcgtgcgtc ggtcgtcgcc gaaggccttc gcgtcggcct cgatctcgcg
      121 gatcaccgtg cgcttgagag ccgaggggct cgccagcaat tcgccgaggc gcgcttcctc
      181 gccgcgcaag tccttcagct cctgctcgat ccggatcgcc tcgagccggg ccagctggcg
      241 cagccggatc tccaggatgt catcggcctg gcgctcgctc agcgcgaagc gcgcgatcaa
      301 ggccgccttc ggctcgtcgg cggcgcggat gatggcgatg acctcgtcga tgtgcaggag
      361 caccaggtgg cgaccttcga ggacgtggat gcgctcggcg accctgccga ggcgatgacg
      421 cgagcgccgc tcgacggtaa gaaggcgaaa gccgttccac tcgacgagca tccggcgaag
      481 actcttctgc gtcggtcgac cgtcggcgcc gaccatcgtc aggttgatcg gcgtcgagct
      541 ttcgaggctg gtgtgcgcga gcagcgtcgt cagcaattcg ctctgctcga tgcggctggt
      601 cttcggctcg aagacgaggc gcaccggagc gtcgcggctc gactcgtcgc gcaccgcgtc
      661 gagcaccgcc aggacggtcg ccctcaactg caactgttcg gcgccgagcg tcttcttccc
      721 ggcccgcagc ttcgggttgg tgagctcctc gatctcctcg agcaccttct gcgcgctggt
      781 gccgggcgga agctcggtga cgacgacctg ccactggccg cgggccaggt cttcgatctt
      841 ccagcgcgca cgaaccttca gcgtgccgcg tccgctcgcg taggcggcac ggatctcgtc
      901 gtcggcgctg atgatctggc cgccaccggg aaaatccgga cccggcagca gccggcccaa
      961 ctcgtcgtcg ccgagcttct cgtcgcgcag cagcgccact gccgcggccg cgacctcgcg
     1021 caggttgtgg ctggggatct ccgtggccag cccgacggcg atgccgctgg cgccgttgag
     1081 aagaacgaac ggcaggcgcg ccggcagcag cctcggctct tcggtcgagc catcgtaatt
     1141 ggcgatgaag tcgaccgtgc cgtcgtcgag ctcgtcgagg aggaggcgcg agatcggcgc
     1201 caggcgcgcc tcggtgtagc gcatcgccgc ggcaccgtcg ccgtcgcgcg agccgaagtt
     1261 gccctggccg tcgaccagcg gatagcgttg cgagaagtcc tgcgccatgc gcacgagggc
     1321 gtcgtacgcg gcctggtcgg aatggggatg gaagcggccg agaacgtcgc cgacgacgcg
     1381 ggcgcttttc acggccttcg ggccggcgcc ggtcgtgaag gccagcccca tgcgcgccat
     1441 cgcgtagagg atccgtcgct gcaccggctt ctggccgtcg ctcacgtcgg gaagagcgcg
     1501 ccccttgacg acggaaagcg cgtattcgag ataggcgcgc tcggcgtatt caccgagagc
     1561 gacggcgtcg ccgtcggcgg cggcaggtgg cgaatcgaaa aggtcgggag tcacttggga
     1621 gcggaatcag gaagatgtga acgggacggc gaatcgtcgt cggccctcgc aacgcgattg
     1681 tcgccaagcg gcgatccgtg gcccgcaggc ggtcatgctg cgacccggga cgcgtcgcct
     1741 cgtggcgacg cgacgccgcg acacgaacgc cggcagcgcc aagcgccccg gacggcgaag
     1801 ctcaggccgc ggcgacgagc cccgccggca gcggcgggtc cgggacggtg cggccggtcg
     1861 tcggctggcc cttgaaatcg agccagacgt gatgctcgta gtcgtagacc ttgcaccgcc
     1921 gcatgatgtc gcccagcacc cggggcgaca gcggcaccgc cagcgcccgg tcaccgagca
     1981 attcattgag cctggcctgc gccgcgcgga ggcgatagca cggaatggcc gggacgacgt
     2041 gatgcgccgg gtgctcgcag atgtcgtgcg tcactgagct gagaaaggcc gggatgcgga
     2101 catgcacggt cagcgcctcg ggtccgaagt cttccatccg agcgtcgccc ggaccgaacc
     2161 acgggatctg cgggtgcgta tgctggaaat agaggatcgc cgcctgcagc gtctgcatga
     2221 tgaagaacgg caagccgatg acgagaacgg cgtcggcgag caggccgatc gcatcgccgc
     2281 cgttgcgacg cgccaggcag gcgacgatcg cgcagagata ggcgacgagc agtgcgccga
     2341 aggcccaggc ctgggcgcga aacttcggcg tcatcgcctt gttgggcagc acttcggctc
     2401 gcaaccagcg cgacaggatg ccgtagggtg cgaaggcgag cacgtggggg gcgcggatga
     2461 agcgttccca gaggcgccgc catgccggcg cgttgcagta ctcctgccac gaaagcggcc
     2521 ggtacacgtc gggctgcggc ccgttgaggc cgggatggtg gccgatcacc aggtggtcgt
     2581 accagcggat gcggtagttg tgcaggctca ccagatagcc gatcgcgctc agcaccttgt
     2641 tcaggcggct cgaggcggtc agcgagttgt gcacggcgtc gtgggccagc gtgtagaggc
     2701 tgcccatctt caggccgacc agcaccgcgc ccaaaacgcg ccaggccggc tgcgacgcga
     2761 agagcgcgag cgacagaccg gctgcgtaga cgacgaaatc gacgaggaag aggcgaacgc
     2821 cgatgcgcgt gtccggcacg gcgtatccca tcaaggccct ggccagctcc tgtcgagtcg
     2881 gtacgagatc gctgtctgcg ctcatcgcgg ggcccctcct gacggcgcga ttatggcgcg
     2941 ccgcggatca cgccgggaag cgctcccgca gcttcagctt ccagaatttc ccggtcgagg
     3001 tgcgcggcac ggcgtcgatc gattcgtagc gatcgggcag ctgccacctg gcgaagccgt
     3061 gcgcaagcag gtgcgcgtcg agcgccgcgg ggtcgacggc ctgtccgggc cgagggacga
     3121 cgcaggccag cggccgctcg ctccacttcg ggtcgggaat cgcaatgacc gccgcctcgg
     3181 cgatcgacgg gtggcccatc agcgcgtttt cgagatcgac cgacgagatc cattcaccgc
     3241 ccgacttgat gaggtccttg gtgcggtcgg tgatccggat gaagccgagc tcgtccatcg
     3301 aggcgacgtc cccggtgcgc agccagccgt cgtcggtgaa tttctcgtcg gtcaccggca
     3361 cctcgtggta cgaaccggtg atgaacggcc cgcgacactg gatctcgccc atcgccgtgc
     3421 cgttccacgg ggcgtcgtgt ccgtcatcgg cgcggatccg gatgtcgacc agcggcaccg
     3481 gcacgccggc catcgcggca cgccggaatt gttcgtcctc gcccttcccg cgaagctcga
     3541 cacgcgggta ggagacgctc gccagcggcg acgtctcggt cattccccag ccctggacga
     3601 tcgacacgtc gtggcgcgcg aatgcccgga tcagcgattc aggcactgca gccccgccga
     3661 ccagcgaacg catgcccgcc ggcagcgtcc agcgaccggg ttgctcgacg agcgcgcgtt
     3721 cgtacgcctg gatcagcccc atccagatcg tcggcacgcc gagcgagagc gtcggcggct
     3781 cgatctgcat caggtcgagc agatcgtcgg gatgcagatg cgggccggga aagaccagct
     3841 tcacgccgag catcaccgcc gcgtacggca agccccagct gttggcgtgg aacatc
//
LOCUS       DASRWS010000679         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_279808, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000679 DASRWS010000000
VERSION     DASRWS010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279808"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFF72_12975"
     CDS             complement(<1..337)
                     /locus_tag="VFF72_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZV94125.1"
                     /translation="MTSGRPDRYQDLRDAVRVLCAGFPAEYHRDVDARRAYPEAFVEA
                     LTKAGWLAALIPQEYGGSGLGLGEASVIMEEINRSGGNAGACHGQMYNMGTLLRHGSA
                     EQKARYLPRI"
     gene            complement(334..1344)
                     /locus_tag="VFF72_12980"
     CDS             complement(334..1344)
                     /locus_tag="VFF72_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZV94126.1"
                     /translation="MTEARTAATGSGAGHDEVAPSDLDAWIGRTEERRDTVTAVPMAA
                     LAASLDRDEPMPRVGTELPALWHWLYFLPLARQGELGPDGHPKRGGFLPPVPLPRRMW
                     AGSRLVFDRPLRVGDDIARVSRIASIVRKEARSGPLVFVTVQHEIAGPAGTAIHEEHD
                     IVYRDAASTPRRAASPALTSSAPFPPSRRTAPSESSAPLPPSDPTEPIARAGCCDAVF
                     SRVIVPNEVLLFRYSALTFNGHRIHYDRRYATEVEGYPGLVVHGPLIATLLLELLRRE
                     QPRATVSRFEFRALRPIFDVHPFTVCGRPDGVGRFALWVRDAEEVLAMRASAEVGQTR
                     ST"
     gene            complement(1350..2789)
                     /locus_tag="VFF72_12985"
     CDS             complement(1350..2789)
                     /locus_tag="VFF72_12985"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZV94127.1"
                     /translation="MSEATDLADCAAHELLALYRSRKASPVEATRAVLARIERLDPVL
                     NAFCLRAPEEALRAAAASESRWNRGEPSGDLDGVPVSIKDLILVKGWPTLRGSRTVDP
                     KQAWDVDAPVSARLREAGAVLLGKTTTPEFGCKGETNSPLTGITRNPWNPAKTPGGSS
                     GGTAAAVAAGMGPLSVGTDGAGSVRIPAAFCGNFGLKPSFGRVPAYPLSPMGTVAHLG
                     PHTMSVVDAALMMNVLKRPDARDWTSLPPDAADYVHGLDAGVAGLRIAFSPTLGYAAH
                     VDGEVAAAVAAAVRDLEALGAIVENVDPGFADPLEITTGLWFSGVWAIWSALTPAQQA
                     VCDPDFRAEAELGAAYGVLDVHRLQLRRGELGSHMRQFMARFDLLVTPTVAVPAFDAK
                     PAGHSPMDPAAMLGWTPFSYPFNLTQQPACTIPCGLTRAGLPIGLQIVGRMFDDAGVL
                     RAARAYERMHPIPRPTAARADSDAGPRRA"
     gene            complement(2895..>3108)
                     /locus_tag="VFF72_12990"
     CDS             complement(2895..>3108)
                     /locus_tag="VFF72_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94128.1"
                     /translation="LGPHRRARFDRALVRGSARIGSFVDAVFAWPSARRRARREADEA
                     IRRARQGEWSGNVYRPKSFRDKRKLH"
BASE COUNT          473 a         1095 c         1133 g          407 t
ORIGIN      
        1 cgatgcgtgg caggtagcgc gccttctgct ccgcggagcc gtggcgcaag agcgtcccca
       61 tgttgtacat ctgcccgtgg caggcgccgg cgttgccgcc gctgcggttg atctcctcca
      121 tgatcacgct cgcctcgccg aggccgaggc ccgagccgcc gtattcctgc ggaatcagcg
      181 cggcgagcca gccggccttc gtcagcgcct cgacgaaggc ttcggggtag gcgcgtcggg
      241 cgtcgacgtc gcggtggtat tcggcgggaa acccggcgca cagcacgcgt acggcgtcgc
      301 ggaggtcctg atacctgtcg ggccggccgc tcgtcatgtc gatcgcgtct ggccgacctc
      361 ggcgctggct cgcatcgcca gcacctcttc ggcgtcgcgc acccacaggg cgaagcggcc
      421 gacgccatcg ggccggccac agaccgtgaa cggatggacg tcgaagatcg gcctcagggc
      481 cctgaactcg aagcgcgaca cggtggcgcg aggctgctcg cgacgcagca gctcgagcag
      541 cagcgtggcg atcagcgggc cgtggacgac gaggccgggg tagccctcga cctcggtggc
      601 gtagcggcga tcgtaatgaa tgcggtggcc gttgaaggtg agcgccgaat agcgaaagag
      661 cagcacctcg ttcggcacga tcacgcgcga gaagacagcg tcgcagcatc ccgcgcgcgc
      721 gatcggttca gtcggatcag agggcggcag cggtgccgac gactcggacg gcgcggtccg
      781 tcgggacggc gggaacggcg cggacgacgt caaggccggc gacgcggccc gacgcggtgt
      841 cgacgcggcg tcgcgataga cgatgtcatg ctcctcgtgg atcgccgtcc ccgccggtcc
      901 ggcgatctcg tgctgcacgg tgacgaagac gagcggtccg gagcgcgcct ccttgcgaac
      961 gatcgaggcg atgcgcgaga cgcgcgcgat gtcgtcgccg acgcgaagcg gacggtcgaa
     1021 gacaaggcgg ctgcccgccc acatgcgacg cggcaagggc actggcggca ggaagccgcc
     1081 acgcttcggg tggccgtcgg gcccgagctc gccctggcgc gccagcggca ggaagtagag
     1141 ccagtgccaa agcgcgggca actccgtgcc cacgcgcggc atcggctcat cgcgatcgag
     1201 cgacgccgcc agcgccgcca tcggcaccgc cgtcacggtg tcgcgacgct cttcggtccg
     1261 gccgatccag gcgtcgagat cggaaggcgc gacttcgtca tgtccggcgc cggaacccgt
     1321 ggctgccgtg cgggcctcgg tcatcgggat caggcgcgac gcggccccgc gtcggaatcc
     1381 gctcgagcag ctgtcggccg cggaatcgga tgcatccgct cgtaggcgcg cgcggcgcgc
     1441 agcacccccg cgtcgtcgaa catccggccg acgatctgca ggccgatcgg caggccggcc
     1501 cgcgtcaggc cgcagggaat cgtgcaggcc ggctgctggg tgaggttgaa cggatagctg
     1561 aagggcgtcc aaccgagcat cgccgccggg tccatcgggg agtgaccggc cggcttcgca
     1621 tcgaaggcgg gcaccgcgac cgtcggcgtc acgagcagat cgaagcgcgc catgaactgg
     1681 cgcatgtgcg atccgagctc tccacgcctc agttgcaggc gatgcacgtc gagcacaccg
     1741 tacgccgcgc cgagctcggc ctcggcgcga aagtcgggat cgcagacggc ctgctgcgct
     1801 ggcgtgagcg cgctccagat cgcccagacg cccgagaacc agaggcccgt cgtgatctcg
     1861 agcggatcgg cgaagcccgg gtcgacgttc tcgacgatcg cccccagcgc ctcgagatcg
     1921 cgaaccgcgg cggcgacggc cgcggccacc tcgccgtcga cgtgagccgc atagccgagc
     1981 gtcggcgaaa acgcgatgcg caggccggcg actcccgcat cgagcccatg cacgtagtcg
     2041 gcggcgtcgg gcggcagcga agtccagtcg cgggcatcgg ggcgcttcag cacattcatc
     2101 atcagcgccg cgtcgacgac gctcatcgtg tgcggcccga gatgggcgac cgtgcccatc
     2161 ggcgacagcg gatacgccgg cacgcgaccg aagctcggct tcaggccgaa gttgccgcag
     2221 aacgcggccg ggatgcgcac gctgccggcg ccgtcggtgc cgaccgacag cgggcccatg
     2281 ccggccgcca ccgccgcggc cgtgccgccc gacgagccgc ccggcgtctt cgccggattc
     2341 cacggattgc gggtgatgcc ggtgagcggc gagttggtct cgcccttgca gccgaactcg
     2401 ggcgtcgtcg tcttgccgag gagcaccgcg ccggcctcgc gcaaccgtgc cgacaccggc
     2461 gcatcgacgt cccaggcctg cttcggatcg accgtgcgcg agccgcgcag cgtcggccag
     2521 cccttgacga gaatgagatc cttgatcgag accggcacgc cgtcgagatc gcccgacggc
     2581 tcgccgcgat tccagcgcga ttcgctcgcc gccgccgcgc gcagcgcttc ttcgggagcg
     2641 cgaagacaga atgcgttgag caccggatcg aggcgctcga tgcgcgccag caccgcccgt
     2701 gtcgcttcca ccggcgaggc cttgcgcgag cgatagaggg ccagcagctc gtgcgcagcg
     2761 cagtcggcga gatcggtggc ttcgctcatc ggacgactcc cggtgcgggc gcaggcccgc
     2821 gtccggccga ttgtgatccg gcgcatggcg gtgccgcgag acgcaccgac ggcgcgatcg
     2881 tccggcctgc cgcgtcagtg cagcttgcgc ttgtcgcgaa acgacttcgg gcggtagacg
     2941 ttgccgctcc actcgccctg acgggcccgg cggatcgcct cgtcggcctc gcgccgggcg
     3001 cggcgacgtg ccgacggcca ggcgaagacg gcatcgacga acgagccgat gcgcgcgctc
     3061 ccgcggacga gtgcgcgatc gaagcgggcc ctccggtgcg ggccgagc
//
LOCUS       DASRWS010000680         6132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_280147, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000680 DASRWS010000000
VERSION     DASRWS010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280147"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFF72_12995"
     CDS             <1..645
                     /locus_tag="VFF72_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZV94129.1"
                     /translation="VDVVRGVSGSVAAGEVLCVIGRNGVGKTTLLKMIFGEVACRAGE
                     IRLDGRSIDRLEPVHRRAAGISYCPQERPVFDNLSVRDNLTLMRAGRSLDAFAASFAR
                     FPILPRRLAQHAGTLSGGEKKILSFVRGLAEAQPLLLLDEPSEGVQWENIVHMTEAIG
                     EAKSRGTALVVVEQNLAFAELIADAWLVVEHGTTVLAARKGEVERDRLLQHLHV"
     gene            863..1243
                     /locus_tag="VFF72_13000"
     CDS             863..1243
                     /locus_tag="VFF72_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94130.1"
                     /translation="MSDIRIIAVSLEGGEQLQHITHVWTVGGAMPRQRAVDDLWARRC
                     RYYTVAPTGRIDVLALPRRGPGIVAFLLRRRVRGILESARGASATDSLLTLPRLPSPT
                     RPRRSRAMAMAAAAMGGDTESAGA"
     gene            complement(1322..2725)
                     /locus_tag="VFF72_13005"
     CDS             complement(1322..2725)
                     /locus_tag="VFF72_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV94131.1"
                     /translation="MAVVLLGIAMSVLDATIVNLALPGIVRSLHAEASSAVWVITAYQ
                     VAKLVMLLPCAMLGDLFGPRGVYLWGLSLFTLSSLACAFSPSLAVLIAARIAQGLGAG
                     GLMSVNGALIRVIYPRRALGRGIALNSVVVATSSVAGPSIAAGILSIASWPWLFAVNV
                     PIGLLVVVLGRRCLPGHPAAAPKAMRLRPLDVVLNAAMFGLAFLGADVLGSHAAGAAS
                     SSVPGTGVALLVGALVVGVVYIARQRRQPLPLFPLDLLRIPLFALSMCTSVTAFAAQM
                     IAYVALPFLLLEALGRSPAAAGLLITAWPIATVVAAPIAGRLIGRVPDGLHGAVGLWI
                     LACGLAAMAMLGPHPGNADIAWRMALCGLGFGFFQSPNNHTILTSAPPARSGAASGML
                     GTARLTGQSIGSVLFGVILGALGAESMRGPSLALGLAAAFAAVAGVFSGLRLRARGVA
                     GTGSPSAEARATSSVVG"
     gene            3201..3806
                     /locus_tag="VFF72_13010"
     CDS             3201..3806
                     /locus_tag="VFF72_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94132.1"
                     /translation="MSARPPGRTWLRSKPARNDDDAGESAGKLRWARSLLTRSVGLEK
                     RGKQLHVVLVERDRGPGARPSASPLQQMRDELQARLLVHDPSTQAIRNLYVVYKELSG
                     DGWPAVEMLPHGLTQLAQVEAEILQTQEPSPVLAGIIERLVAVNAATAQRIELEAEVP
                     VLDDEATEHWAVTDIPEVSEASHEEYERMERSWIGTVPAEL"
     gene            complement(3937..5235)
                     /locus_tag="VFF72_13015"
     CDS             complement(3937..5235)
                     /locus_tag="VFF72_13015"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZV94133.1"
                     /translation="MSHLIVHGGRPLRGTVTPSANKNAVLPVLCASLLTDQPVVLHRV
                     PDITDVRKLLSFFAELGSSVEMDFASGTLRLQHAARIDGVAVRLPTGMRSSIMLVPAL
                     LHRLGRARLEDDVTGCTLGVREIDPHLQVFFGFGAEVEPQPGATTIRAPRRLAASDQW
                     LDYASVTTTENFVLCAALANGRSQLVNAACEPHVQEFCAFLGKMGARITGCASSHLQV
                     VGVAELGAAEHTFEDDFHEIATFLALGAITGGDVVVRNGSMAQFPLLDRTFAKFGVEI
                     EHRDGYSSAHVSGPLRVRPPITQHVLQKVEAAPWPYVPADLLPIFVALGVRAEGSAMF
                     WNKVYEGALGWSAELGKFGAHALLCDPHRLIVYGGKPLQPATVESPYIIRVAIALFMI
                     AASIDGRSRILNALPIRRAHPNFVGNLNALGARAEWSEGD"
     gene            5380..>6132
                     /locus_tag="VFF72_13020"
     CDS             5380..>6132
                     /locus_tag="VFF72_13020"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZV94134.1"
                     /translation="MTFLHSTSTPLAHQVLELLAYFVGARLYFASARRQPRPGPALDR
                     LLLVASAAFGAMLGSKLLHVAEHLPALRGQPLAVWLGGKSLLGGFLGGTAAVEMAKRS
                     IAWKPSTGDAWVPAIVVGIIIGRIGCQLSGLWDQTYGNPTDLPWAWDYGDGVPRHPVA
                     AYEIVLVACAWLGVSRMHGKAGQRFAALMAAYCLIRLCLEFLKPPFAVEAAGTLPTTL
                     LAGLTAIQWAAIVGLGYFGWRVFMLRPADHGNS"
BASE COUNT          977 a         2143 c         2186 g          826 t
ORIGIN      
        1 gtcgacgtcg tgcgcggcgt ctccggcagc gtcgccgccg gcgaggtctt gtgcgtgatc
       61 ggtcgcaacg gcgtcggcaa gacgacgctc ctgaagatga tcttcggcga ggtcgcctgt
      121 cgcgccggcg agatccgcct cgacggtcga tcgatcgacc ggctcgagcc ggttcaccgg
      181 cgcgccgccg ggatcagcta ctgcccgcag gagcggccgg tgttcgacaa cctgagcgtg
      241 cgcgacaacc tgacgctgat gcgggccggg cgcagcctcg acgccttcgc ggcgtcgttc
      301 gcgcgatttc cgatcctccc caggcgcctc gcccagcacg ccggcaccct gtcgggcggc
      361 gagaagaaga tcctttcatt cgttcgcggg ctggccgagg cgcaaccgct gctcctcctc
      421 gacgagccga gcgaaggcgt gcaatgggaa aacatcgtcc acatgaccga ggcgatcggc
      481 gaagcgaaat cgcgcggcac cgctctcgtc gtcgtcgagc agaacctcgc cttcgccgag
      541 ctgatcgccg atgcgtggct cgtcgtcgag cacggaacga ccgtgctggc ggcgaggaag
      601 ggcgaggtcg aacgcgaccg tctgctgcag cacctgcacg tctgacggcc ggccggctgg
      661 cttcggcgcg aggcgaggtc gaagcgcttc ggccgccccg tccttccacg tcgttccgca
      721 tcgctcgacg cggcgaggcg agccctgcgt cccggcaaaa agagaagcgc ttcacgcttt
      781 tccacgcatc gcgcccggcg gcacatccag ccgcggcccc gtcgctacga tttgttgcga
      841 atggcttgtc ggggggttcg ccatgtcgga catacggatc atcgcggtct cgctcgaggg
      901 aggcgagcaa ctgcagcaca tcacgcacgt ctggaccgtc ggcggcgcca tgccgaggca
      961 acgggcggtg gacgacctgt gggcccgacg ctgccgctac tacacggtcg cgccgaccgg
     1021 gcgcatcgac gtcctcgcgc tgccacgacg cggcccggga atcgtcgcct tcctgctgcg
     1081 gcggcgcgtc cgcggcatcc tcgaatcggc gcgcggtgca tcggcgaccg acagcctgct
     1141 gacgctgccg aggctgccca gcccgacgcg tccgcggcgc agtcgggcga tggcgatggc
     1201 cgcagccgcg atgggaggcg acaccgaaag cgcgggcgcc tgacggggcg cggtcgactc
     1261 cgggactgca agaccgcgaa accgcacggc ggcaacaccg cgaccgaacg gccgcggcgg
     1321 ctcagccgac gacgctcgac gtcgcccgtg cttctgcgct tggtgacccc gtccccgcga
     1381 cgccccgggc ccgcagtctc aagccgctga agacgcccgc gaccgccgcg aacgcggcag
     1441 caagacccag cgcaagactc ggtccgcgca tgctttcggc accgagcgcg ccgaggatca
     1501 cgccgaacag caccgatccg atcgactgcc ctgtcagtcg cgccgtgccg agcatgccgc
     1561 tggccgcgcc gctgcgcgcg ggcggggccg aggtcaggat ggtgtgattg ttcggcgact
     1621 ggaaaaagcc gaagccgagc ccgcacagcg ccatccgcca ggcgatgtcg gcgtttcctg
     1681 gatgcgggcc gagcatcgcc atcgccgcca acccgcacgc cagtatccag agtcccacgg
     1741 cgccgtgcag gccgtccggg acgcggccga tcagccgccc cgcgatcggc gcggcgacca
     1801 cggtggcgat cggccatgcc gtgatgagca gcccggccgc cgcgggcgac cggcccaagg
     1861 cctcgagaag aaggaacggc agcgcgacgt aggcgatcat ctgcgcggcg aacgccgtga
     1921 ccgacgtgca catcgacaac gcgaagagcg ggatgcgaag gaggtcgagc gggaagagcg
     1981 gcagcggctg acgacgttgc cgcgcgatgt agacgacgcc gacgaccagg gcgccgacca
     2041 gcagcgcgac cccggtgccg ggaaccgagc tcgaggccgc tcccgcggcg tgcgagccga
     2101 gcacgtcggc gccgagaaag gccaggccga acatcgctgc gttgaggacg acgtcgagcg
     2161 gccgcagcct catcgccttc ggcgcggcgg cgggatggcc gggcaggcag cggcggccga
     2221 ggacgacgac gagcaggccg atcggcacgt tgaccgcgaa cagccacggc cacgaagcga
     2281 tcgacaggat gccggcggcg atcgaagggc ccgccaccga cgaggttgcg acgaccaccg
     2341 agttcaaggc gatgccgcgg ccgagggccc ggcgcggata gatcacccgg atcagcgcgc
     2401 cgttcaccga catcagtccg cccgcgccca ggccctgggc gatgcgcgcc gcgatcagca
     2461 ccgccagcga tggcgagaag gcgcaggcaa gcgacgacag cgtgaaaagc gaaagacccc
     2521 agagatagac gccgcgcgga ccgaacaggt cgccgagcat cgcgcacggc agcagcatga
     2581 ccagcttcgc cacctggtac gcggtgatga cccagaccgc cgaggacgct tcggcgtgca
     2641 acgaacggac gatgccgggc agtgccaggt tgacgatggt ggcgtcgagc acggacatcg
     2701 cgatgccgag caggaccacc gccatcgcgc catagcgctg cggaatcggc aggccgtcgc
     2761 tcaggctgtc aacaagggaa tcggcaggag acaagggcgc gtcgctcggc gcaccttccc
     2821 gtcggagggc gcggcgtgtg gtcggtggag ggctcgaagc aactcgcggg gcgacccggt
     2881 ctgttcgctt cggccatcgc gattctgcta cggaggcgac gtccccgctg ccgacgcgga
     2941 cggcgcggcg gccggcgcgc catcgccccg gcggccggcg cacgcgcgcc gcgacctcgc
     3001 ttgatgcgta gtcgttcccg gcacagggcg ggcgcgacac gcccgcacgc cggcgtcggg
     3061 ccgcccgtcg ccctcggcga gagcgggacg tgcgtttccg cacggctcgc cgcagcagcc
     3121 gccgggctga cctcaagccg gtcggcgccg cgccgatgcc gctgatacga cgcacgtcga
     3181 acatcgaaaa gggacgccga atgtccgcac gacccccagg ccgcacatgg ctgcgttcca
     3241 agccggccag gaacgacgac gatgccggcg aatcggccgg caagctgcgc tgggcgcgca
     3301 gcctgctcac acgatcggtc gggctcgaga agcgcggcaa gcaactgcac gtcgtcctgg
     3361 tcgagcgcga ccgcggcccc ggcgccaggc cctcggcgtc gccgctgcag cagatgcgcg
     3421 acgagttgca ggcccgtctt ctcgtccacg acccgtcgac gcaggcgatc cgcaacctct
     3481 acgtggtcta caaggagctt tcgggcgacg gctggccggc cgtcgagatg ctgccgcacg
     3541 gcctcacgca gctcgcccag gtcgaagcgg agatcctgca gacgcaggag ccctcgcccg
     3601 tgctggcagg aatcatcgag cggctggtcg ccgtcaacgc cgcgacagcg caaaggatcg
     3661 agctcgaggc cgaggttccc gtcctcgacg acgaggcgac cgagcactgg gccgtcaccg
     3721 acatccccga ggtctccgag gcctcgcacg aggaatacga gcggatggaa cgaagctgga
     3781 tcggcaccgt gccggccgag ctgtagccgg acgcgccgcg gcgagcgggc gcgctttgtc
     3841 gtcgcgagcc tcgggcggcc gatgccgccg atcatgcagg cgccggccac tcctccggag
     3901 ccgatgtcgc ggccggtccg gccgagccgg cctccgtcaa tcgccttcgc tccactcggc
     3961 ccgggcaccg agcgcgttga ggttgccgac gaagttcgga tgcgcacgcc ggatcggcaa
     4021 ggcgttgagg atgcgcgatc gtccgtcgat gctggccgcg atcatgaaga gggcgatggc
     4081 gacgcggatg atgtaggggc tctcgacggt cgccggctgc agcggcttgc cgccgtagac
     4141 gatgaggcgg tgcgggtcgc acagcaacgc atgcgcgccg aacttgccga gctcggcgct
     4201 ccagccgagc gcgccttcgt agaccttgtt ccagaacatc gccgatcctt cggcgcgcac
     4261 gccgagcgcg acgaagatgg gaagcaggtc ggccggcacg tacggccagg gcgccgcctc
     4321 gaccttctgc agcacgtgct gggtgatcgg cggccgcacc cgcagcggcc cgctcacgtg
     4381 cgccgagctg tagccgtcgc ggtgctcgat ctcgacgccg aacttggcga aggtgcggtc
     4441 gagcaacggg aactgcgcca tcgagccgtt gcgcaccacc acgtcgccgc ccgtgatcgc
     4501 gcccagggcg aggaaggtgg cgatctcgtg gaaatcgtct tcgaaggtgt gctcggcagc
     4561 gccgagctcg gccaccccga ccacctgcag atgcgagctc gcgcagcccg tgatgcgcgc
     4621 gcccatcttg ccgagaaagg cgcagaactc ctgcacatgc ggctcgcacg ccgcattgac
     4681 gagctgcgag cggccgttcg ccagcgcagc gcagagcacg aagttttcgg tcgtcgtcac
     4741 cgacgcgtag tcgagccact ggtcgctcgc ggcgaggcgg cgcggcgcgc ggatcgtcgt
     4801 ggcgcccggt tgcggctcga cctcggcgcc gaatccgaag aagacctgca ggtgcggatc
     4861 gatctcgcgc acgcccagcg tgcagccggt cacgtcgtct tccagccgcg cccgtcccag
     4921 ccggtgcagc agcgccggca ccagcatgat cgacgagcgc atgccggtgg gcagccgcac
     4981 cgcgacgccg tcgatgcggg cggcgtgctg cagacgcagc gtgccgctgg cgaaatccat
     5041 ctcgaccgag gagccgagct cggcgaagaa cgacaggagc tttctcacgt cggtgatgtc
     5101 gggcacgcgg tgcaggacga cgggctggtc ggtgagcagg ctcgcgcaga gcacgggcag
     5161 caccgcgttc ttgtttgccg atggcgtgac ggtgccgcgc aacgggcggc cgccgtggac
     5221 gatcagatgg ctcatgtccg gcctcgaggg agtgacgaca ggctgccagt ctcgcgggcg
     5281 ggggtgaacg cgccatgaag tgtgtgaagc gacgatgaat cgcctcgccg atgagcgaag
     5341 atcggtgcct gatccatcgc gaccgtgccg gaacgaggcg tgactttcct tcattcgacc
     5401 tcgacgcccc tggcgcatca ggtgctcgaa ctgctcgcct atttcgtcgg tgcgcggctg
     5461 tactttgcaa gcgcgcggcg gcagcctcgg ccggggcctg ccctcgaccg gctgctgctg
     5521 gtggcgagcg ccgcgttcgg cgcgatgctc ggcagcaagc tgctccacgt cgccgagcac
     5581 ctgccggcgc tgcgcggcca gccgctggcc gtgtggctgg gcggaaagtc gctgctcggc
     5641 ggctttctcg gcggtaccgc ggccgtcgaa atggcaaagc gatcgatcgc ctggaagccg
     5701 tcgaccggcg acgcctgggt tccggcgatc gtggtcggca tcatcatcgg ccggatcggt
     5761 tgccagttgt cgggactctg ggaccagacc tacggcaatc cgaccgacct gccctgggcg
     5821 tgggactacg gcgacggcgt gccgcgtcat ccggtcgcgg cgtacgagat cgtcctggtc
     5881 gcttgcgcct ggctcggcgt atcgagaatg cacggcaagg ccggccagcg gttcgcggcg
     5941 ctgatggcgg cgtactgcct gatccggctg tgcctcgaat tcctcaagcc gcctttcgcg
     6001 gtcgaggccg cgggcacgct gcccaccacc cttcttgccg ggttgaccgc gatccagtgg
     6061 gcggccatcg tcgggctcgg ctatttcggc tggcgcgtct tcatgcttcg gccggccgat
     6121 cacggcaact cc
//
LOCUS       DASRWS010000681         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2568277, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000681 DASRWS010000000
VERSION     DASRWS010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2568277"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..493
                     /locus_tag="VFF72_13025"
     CDS             2..493
                     /locus_tag="VFF72_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZV94135.1"
                     /translation="MVDSHHAAPTVTEPRDTLERTDFLPFKLLSDLPWAMTAHVVYEA
                     VDPGAVLTVSARGVKDVVRCHIGFDGLLLSDDLSMQALGGSLADRTRRALAAGCDIAL
                     HCNGRMDEMREVVEESGAMTDAALRRFRAGRGHLARYRAPLGAADLVDAATRLTSLLP
                     EWG"
     gene            549..1358
                     /locus_tag="VFF72_13030"
     CDS             549..1358
                     /locus_tag="VFF72_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZV94136.1"
                     /translation="MSDTDTPPPAADGFATAGPDVIAPGEGELIVNLEGFEGPLDLLL
                     ALARDQKVDLRRISMVALVDQYLAHVSQARRLRLELAADYLVMAAWLAYLKSRLLLPE
                     PPPGEEPSGQEMADALQWQLRRLEAMQEAGVRLMARPQLGKDVFARGQPEGMVVVRRA
                     IWDVKLYDLLSAYGSQVRPKDADTYQIAPMQFFSVEEAAERLGRMLGIAIDWQTLEAF
                     LPAGLTDPTRRRSAIASTFVAGLQLAKDGQIELRQAERFGPIHLRKRTEQR"
     gene            1355..2422
                     /gene="scpB"
                     /locus_tag="VFF72_13035"
     CDS             1355..2422
                     /gene="scpB"
                     /locus_tag="VFF72_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZV94137.1"
                     /translation="MTVPDMSEREISTPAIEDEAKLAETAVEGADPEKMERVDPVANE
                     DVLVAGKDRPDEESDDDEEEEFDDDEDDGDDDSDDDDDEDDDESESDGESDEDESDED
                     DDDPDEDERAAADVEPLAASTEAPAEAVIVAPVPRTEIEVSDVVSPDHAQHLRLLEAL
                     IFAGAQALDEKELAERLPNDADVARLLADLGEAYASRGVNLVKVAGGYAFRTAPDLSE
                     KLRIEKPVTRKLSRAAIETLAIIAYHQPVTRAEIEQVRGVGLSKGTLDLLFEQNWIRP
                     MGRRRAPGKPVTWGTSDFFLEHFGLASLDDLPGQEEMKAAGLLDARAQPPIFRPEEPD
                     LPLEPTEEEADEPLAADEQGH"
BASE COUNT          444 a          815 c          874 g          378 t
ORIGIN      
        1 gatggtcgat tcgcaccatg cggcgccgac ggtgaccgag ccgcgggaca ccctggagcg
       61 caccgacttc ctgccgttca agctgctgag cgacctgccg tgggcgatga ccgcgcacgt
      121 ggtctacgag gcggtcgacc ccggcgccgt cctgacggtt tcggcacggg gcgtgaagga
      181 cgtggtgcgc tgccatatcg gcttcgacgg cctgttgctg tccgacgacc tgtcgatgca
      241 ggccctgggc ggttccctgg ccgatcgcac gagacgcgcc ctcgccgcgg gctgcgatat
      301 cgccctgcac tgcaacggcc ggatggacga gatgcgcgag gtcgtggagg agtccggcgc
      361 catgaccgac gctgccctcc gccgcttcag ggcgggacgt ggccatctcg cccgataccg
      421 cgcgccgctt ggcgcggcgg atctggtcga tgccgcgacg agactgacct cgcttctgcc
      481 ggaatgggga taaggctggg tcaaatccag tggcggcatg cgcgccggct ggactaaacc
      541 ggggctgcat gagcgatacg gacacccctc cgcctgccgc cgacggtttc gccacggccg
      601 ggcctgacgt catcgcgccg ggcgagggcg agctgatcgt caatctcgag ggcttcgagg
      661 ggccgctcga tctgctgctc gccttggcgc gcgaccaaaa ggtcgatctg cgccgcatct
      721 cgatggtggc gctggtcgac cagtatctcg cccatgtgtc gcaggcacgc cggctcaggc
      781 tggaactcgc cgccgattat ctcgtgatgg cggcctggct tgcctatctg aagtcgcgcc
      841 tgctgctgcc ggagccgccg cccggcgagg aaccgtcggg acaggagatg gccgacgcgc
      901 tgcaatggca gcttcgccgg ctggaggcga tgcaggaagc aggcgtgcgc ctgatggcgc
      961 ggccgcagct cggcaaggat gtcttcgcgc gcggccagcc cgaaggcatg gtcgtcgtgc
     1021 gccgcgccat atgggacgtg aagctctacg acctgctgtc cgcttatggc agccaggtac
     1081 ggccgaagga cgccgatacc taccagatcg cgcccatgca attcttctcg gtcgaggaag
     1141 cggcagagcg gctcggccgt atgctgggca tcgccatcga ctggcagacg ctcgaggcgt
     1201 tcctcccagc cggcctgacc gatccgacgc gacggcgctc ggcgatcgcc tcgactttcg
     1261 tggccggcct ccaattggcc aaggacggcc agatcgagct gcgccaggcc gagcgcttcg
     1321 gccccatcca tctgcgtaaa cgtaccgagc aacggtgacg gtcccggaca tgtcagagcg
     1381 agagatctcc acgcccgcca tagaagacga ggcgaagctt gccgaaacgg ccgtcgaggg
     1441 cgccgacccc gagaagatgg agcgcgtcga tccggttgcg aacgaggacg tcctggtggc
     1501 cggcaaggac aggccggacg aggagtcgga cgacgacgag gaggaggaat tcgacgacga
     1561 cgaagacgat ggtgacgacg attccgacga cgacgacgat gaagatgacg acgagtccga
     1621 gtccgacggg gaatcggacg aagacgagtc ggatgaagac gacgacgatc cggacgagga
     1681 cgaacgggcc gcggccgatg tcgagccgct agcggcttcg accgaggcgc ctgcggaggc
     1741 ggtcatcgtc gcgccggttc cgcgaaccga gatcgaagtg agcgatgtcg tttcccccga
     1801 tcatgcccag catctccgtc tgctggaggc gctgatcttc gccggcgccc aggcgctcga
     1861 cgagaaggag ctggccgagc ggctgccgaa cgacgccgat gtcgcgcgcc tgctcgccga
     1921 tctcggcgaa gcctacgcct cgcgcggcgt caatctcgtg aaggtcgcgg gcggctatgc
     1981 ttttcgcacg gcccctgatc tttccgaaaa gctcaggatc gaaaagccgg tgacgcgcaa
     2041 actgtcgcgg gcggccatcg agacgctcgc catcatcgcc tatcatcaac ccgtcacgcg
     2101 cgccgagatc gagcaggtgc gcggcgtcgg cctcagcaag ggcacactcg acctgctgtt
     2161 cgagcagaac tggatcaggc cgatggggcg ccggcgcgcg ccgggcaagc cggtcacctg
     2221 gggcacaagc gatttcttcc tcgaacattt cgggctggcg agcctcgacg atctaccggg
     2281 ccaggaggaa atgaaggccg cagggctctt ggacgcgcgc gcacaacccc ccatattcag
     2341 gccagaagag cccgacctgc cgctggagcc gacggaagaa gaagccgacg agccactggc
     2401 ggcagacgaa cagggccatt gagcaggcgc gggggcggtc tcgggtcaaa atacggcctt
     2461 attagacttg ttgccgggcc ttccgcgcgg gtgcgataat cgccccgagc g
//
LOCUS       DASRWS010000682         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2297618, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000682 DASRWS010000000
VERSION     DASRWS010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297618"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2764)
                     /locus_tag="VFF72_13040"
     CDS             complement(<1..>2764)
                     /locus_tag="VFF72_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZV94138.1"
                     /translation="GARLRVDLAALLWPDAALPAGRNSLRQRVFRLQRLAGVELVTGG
                     ERLRLADAVGHDLGEVDDRVDTDPHAPVARLLGTLDYGDAPALDEWVSGARERWRRQQ
                     TARIAELASNHEQRGELAAAIGQAQRLVASDPLLEHAHRRLMRLHYLRGDRGAALAAY
                     ERCRSILRDGLGADPDPETDELARTIASSGTATAVRHAAARPLAPPIAVLRPPRVCGR
                     DAARQQLSAAIDGQRVVLLHGEAGIGKSRLLEDAAQARPSLLATSGLAGDARVPCALL
                     ARLAAKALEAGQLRLPTWAMQEMARLLPAIGSPPRSRLEPLRLQQAFALALRSWADAG
                     VTGLLIDDLHHADDASLECLLALATTNTPRIAWVLAVRTHEMPDALSGWLDANRADRV
                     ETLQLRSLDADGVAELLRSLALERFDAESWTDSLVRHSGGNPLFALETLRAMLSLGDA
                     APAAADRQARLPVPAALDALIERRLAGLSEAALRLARVAALAGSDFDADTATRVLDAH
                     PLDIVEPWRELEQAQLLHDGRLVHDLIAEATARALPADIARILHARLAVTLESLGRGA
                     ARVAPHWAAAESWSRAGDTYAQAAQEAQRASRRSDEMQLWEEAAANLDRAGHAGRAFD
                     ARADSVECVIMVRGVAAAMALAARLDADQRTEPQRMRALTVRATACLMSGQAAAAETA
                     ARTALDAAARLGAHWPRFEASRLLAVALAQSGRADEALQTIESFREFVERDGDARQRH
                     HFWADYAYALKAAQRSSETAAALRQAMLSAQELGDFAELATLTSNLALVEGNLGRVEH
                     ALDHARRARSLSDPLGIAVSPAAGAIQLYVSLHEAALGRYSESLAGFERAEQCFGTDR
                     EMIWIGLTANHKASVLIHLGQFARARQALQFEATTTPATAARRLMLQARIARLLGRSD
                     DASIDAA"
BASE COUNT          378 a          949 c         1076 g          361 t
ORIGIN      
        1 cgccgcgtcg atcgacgcat cgtcgctgcg accgagcaga cgggcgatcc gcgcctggag
       61 catcaggcgt cgcgcggcgg tggcgggcgt cgtcgtcgcc tcgaactgca gcgcctggcg
      121 tgcccgcgcg aactggccga ggtggatgag gacgctcgcc ttgtggttcg ccgtcaggcc
      181 gatccagatc atttcgcggt ccgtgccgaa gcactgttcg gcgcgctcga aaccggccag
      241 cgattcgctg tatcgaccga gtgctgcttc gtgcagcgaa acgtagagct ggatcgcgcc
      301 ggcggccggg ctgacggcga tgccgagcgg gtcgctgagc gagcgcgccc gccgggcgtg
      361 gtcgagggcg tgctcgaccc ggccgagatt gccttcgacg agggcgaggt tcgaggtgag
      421 cgtggccagc tcggcgaagt cgccgagctc ctgcgcggag agcatcgcct ggcgcagcgc
      481 cgcggccgtc tccgacgagc gttgcgcggc cttgagggcg taggcgtagt cggcccagaa
      541 atgatgtcgc tggcgagcgt cgccatcgcg ttcgacgaac tcgcgaaagg actcgatcgt
      601 ctgcagggct tcgtcggcac ggccggattg cgccagcgcc acggcgagga gccgagacgc
      661 ctcgaagcgc ggccagtgcg cgcccagccg ggccgctgcg tcgagtgccg tccgcgctgc
      721 cgtttcggcg gcggcggcct gtcccgacat caggcaggcc gtcgcccgca cggtgagggc
      781 gcgcatccgc tgcggttcgg tgcgctgatc ggcgtcgagg cgagcggcga gcgccatcgc
      841 cgcggccacg ccgcggacca tgatcacgca ctcgacgctg tcggcgcgcg cgtcgaaggc
      901 gcgaccggcg tggccggcgc gatcgaggtt cgccgcggct tcttcccaga gctgcatctc
      961 gtcgcttcgc cgcgaggcgc gttgcgcctc ctgcgcggcc tgcgcgtacg tgtcgccggc
     1021 ccggctccac gactccgcgg cggcccagtg cggagcgacg cgcgccgcgc cacgacccag
     1081 cgactcgagc gtgacggcga ggcgcgcgtg caggatgcgg gcgatatcgg cgggcagggc
     1141 gcgcgccgtc gcctcggcga tgaggtcgtg caccaggcga ccgtcgtgca gcagttgcgc
     1201 ctgctcgagc tcgcgccacg gctcgacgat gtccagcgga tgcgcatcga gcacgcgtgt
     1261 ggcggtgtcg gcatcgaagt cggagccggc gagagcggcg acgcgggcca ggcgaagtgc
     1321 cgcttccgaa agaccggcga gccggcgctc gatcagcgcg tcgagcgccg ccggaaccgg
     1381 cagccgggcc tggcgatcgg ccgcggcggg cgcggcgtcg ccgagtgaaa gcatcgcccg
     1441 caaagtctcg agcgcgaaga gcggattgcc gccgctgtgc cggaccagcg agtcggtcca
     1501 gctctcggca tcgaagcgtt ccagggcgag cgatcgcagc aactcggcga cgccgtcggc
     1561 atccagcgac cgcagctgca gggtctcgac gcggtcggcg cggttcgcgt cgagccagcc
     1621 ggaaagtgcg tcgggcatct cgtgcgtgcg caccgccagc acccaggcga tgcggggcgt
     1681 gttcgtcgtg gcgagagcca gcaggcactc gagagaggcg tcgtcggcat gatggagatc
     1741 gtcgatgaga agacccgtca cgccggcgtc ggcccagctg cgcagggcca gcgcgaaggc
     1801 ctgctgcaga cgcaacggct cgaggcgcga acgcggcggc gagccgatcg ccggcagcag
     1861 gcgcgccatc tcctgcatgg cccaggtcgg caggcgcaac tggcccgctt cgagcgcctt
     1921 ggcggcgagg cgggccagca gggcacacgg cacgcgggca tcgccggcca ggccgctcgt
     1981 ggcgagcagg gacggtcgtg cctgcgctgc gtcctcgagc agccggctct tgccgatgcc
     2041 ggcctcgcca tgcagcagca cgacccgttg cccgtcgatc gcggccgaca gctgctggcg
     2101 ggccgcgtcg cggccgcaga cgcgcggcgg ccgcaagacg gcaatcggcg gcgctagcgg
     2161 gcgcgcagcg gcgtgcctga cggcggtcgc ggtaccggag gaggcgatgg tgcgggcgag
     2221 ctcgtccgtc tctgggtcgg gatcggcacc gaggccgtcg cgaaggatgc tccggcatct
     2281 ctcgtaggcg gccagcgccg cgccgcgatc gccacgcagg tagtgcaggc gcatgaggcg
     2341 gcggtgggcg tgctccagca gcggatcgct cgcgacgagg cgttgcgcct ggccgatcgc
     2401 cgccgcgagc tcgccgcgct gctcgtgatt cgaggccagc tcggcgatcc gtgccgtctg
     2461 ctgtcggcgc cagcgttcgc gcgcgcctga aacccattcg tcgagggccg gtgcatcgcc
     2521 gtagtcgagc gtgccgagaa ggcgcgcgac cggagcgtgc gggtcggtgt cgacccggtc
     2581 gtcgacctcg ccgagatcgt gtccgaccgc atcggccagg cgcagccgtt cgccgccggt
     2641 cacgagctcg acgccggcga ggcgctgcag acgaaagacg cgttggcgaa ggctgttgcg
     2701 gccagccggc aatgcggcgt cgggccagag cagggcggca agatcgacac gcaggcgggc
     2761 gccg
//
LOCUS       DASRWS010000683         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3143675, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000683 DASRWS010000000
VERSION     DASRWS010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143675"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /gene="truB"
                     /locus_tag="VFF72_13045"
     CDS             complement(<1..585)
                     /gene="truB"
                     /locus_tag="VFF72_13045"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZV94139.1"
                     /translation="MEPTRAAAPRPRIARRAVHGVLLLDKALGVSSNAALQQAKRLFR
                     AEKAGHTGTLDPLATGLLPICFGAATKFAQVSLEADKRYLATLCFGVTTSTGDGEGEV
                     LRRRDVGFDREALAAACARFTGAIRQVPPMHSALKHQGRALYDYARAGLAIEREARDV
                     TIGSIGIVEGSGGRWLLDVACSKGTYIRTLAEDIG"
     gene            complement(599..964)
                     /gene="rbfA"
                     /locus_tag="VFF72_13050"
     CDS             complement(599..964)
                     /gene="rbfA"
                     /locus_tag="VFF72_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZV94140.1"
                     /translation="MRHKPSIPNRTFRIADQIQRDVSELIRDLKDPRIGMVTIHAVEV
                     TPDYAHATVFFSVLVGDTAQCEQALNEAAGFIRNGLFKRLSIHTVPTLHFRFDRTTER
                     AAELTALIAKANASRAKED"
     gene            complement(1044..>2132)
                     /gene="infB"
                     /locus_tag="VFF72_13055"
     CDS             complement(1044..>2132)
                     /gene="infB"
                     /locus_tag="VFF72_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZV94141.1"
                     /translation="SPFVAVSAKTGQGVDQLLEQVLLQAEVLELKAPKDAMAKGLVIE
                     AQLDKGRGPVATVLVQSGTLKRGDIVLAGSSFGRVRAMLDEDGKPTAEAGPSIPVEIQ
                     GLTEVPQAGDEFMVLGDERRAREIATFRQGKYRDVKLAKRQAANLENMMEQMGQGAKQ
                     VLPLIIKADVQGSQEALAQSLLKLSTDEVKVQIVHAAVGGISESDINLAIASKAVIVG
                     FNVRADAGSRKLAENNGVDIRYYNIIYDAVDEIKAAMTGMLAPEQREETIGTAEIRTV
                     FVASKIGTVAGSMVTAGVVRRGARFRLLRDSVVIYTGEIESVRRMKDDVREVAAGFEC
                     GIKLKNYNDIKEGDLLEFFEVKEVARTL"
BASE COUNT          316 a          713 c          712 g          391 t
ORIGIN      
        1 gccgatgtct tcggcgagcg tgcgaatgta ggttcccttg ctgcacgcga cgtcgagcag
       61 ccaccgacca ccgctgccct cgacgatccc gatcgatccg atcgtcacgt cgcgcgcctc
      121 gcgttctatc gccaggccgg ctcgcgcgta gtcgtagagt gcacggccct ggtgcttgag
      181 ggccgagtgc atcggcggca cttgccggat ggcgccggtg aagcgggcgc aggccgccgc
      241 cagcgcctcg cgatcgaagc cgacgtcgcg tcgacgaaga acctcgcctt caccgtcgcc
      301 ggtcgacgtc gtgacgccga agcagagcgt cgcaagatag cgcttgtcgg cttccaggct
      361 gacctgtgcg aacttggtcg cggcgccgaa gcagatcggc agcaggccgg tcgccagggg
      421 gtcgagggtg cccgtgtggc cggccttctc ggcgcgaaac aaccgctttg cctgctgaag
      481 cgcggcgttg ctcgagacgc cgagcgcctt gtcgagcagc agcacgccgt gcacggcacg
      541 gcgggcgatg cgcgggcgtg gcgctgccgc gcgtgtcggt tccatggcga cgatggcgct
      601 agtcctcttt ggcgcgcgag gcgttggcct tggcgatcaa cgccgtcagc tcggcggcac
      661 gctcggtggt gcgatcgaac ctgaaatgaa gcgtcggcac ggtatggatc gacagccgtt
      721 tgaagaggcc gtttcggatg aagcccgcgg cctcgttgag agcctgctcg cactgcgccg
      781 tgtcgcccac cagcaccgag aagaagacgg tcgcgtgcgc gtagtcgggc gtcacctcga
      841 cggcgtggat cgtcaccatg ccgatgcgcg gatccttcag gtcgcggatc agctccgaca
      901 cgtcgcgctg gatctggtcg gcgatgcgaa aggtccggtt cgggatcgat ggcttgtggc
      961 gcatggaatg gtgtcctcgt tgactcgcaa acaagaaccc cgccgtcttg tggagggcgg
     1021 ggttcggaag gagccgttgc cgcctacagc gttcgcgcga cttccttgac ctcgaagaac
     1081 tcgagcaggt cgccttcctt gatgtcgttg tagttcttga gcttgatgcc gcactcgaag
     1141 ccggcggcaa cctcgcgaac gtcgtccttc atccggcgca ccgactcgat ctcgccggta
     1201 tagatgacga cgctgtcgcg cagcaatcgg aagcgcgcgc cgcggcgaac gacgccggcg
     1261 gtgaccatcg agccggcgac cgtgccgatc ttcgaggcca cgaacacggt gcggatctcc
     1321 gccgtgccga tcgtctcctc gcgttgctcg ggcgccagca tgcccgtcat cgccgccttg
     1381 atctcgtcga ccgcgtcgta gatgatgttg tagtagcgga tgtcgacgcc gttgttctcg
     1441 gccagctttc tcgatcccgc atcggcgcgc acgttgaagc cgacgatgac cgccttcgat
     1501 gcgatcgcga ggttgatgtc gctctcgctg atgccgccca ccgccgcgtg cacgatctgc
     1561 accttgacct cgtcggtcga cagcttgagg agcgactgcg cgagcgcttc ctgcgagccc
     1621 tgcacgtcgg ccttgatgat gagcggcagc acctgcttgg cgccctgccc catctgctcc
     1681 atcatgttct cgaggttcgc ggcctggcgc ttggcgagct tgacgtcgcg gtacttgccc
     1741 tggcggaagg tggcgatctc gcgggcacga cgctcgtcgc cgagcaccat gaactcgtcg
     1801 cccgcctgag gcacttcagt gaggccctgg atctcgaccg ggatcgacgg cccggcctcg
     1861 gcggtcggct tgccgtcttc gtcgagcatc gcgcgaacgc gcccgaagct cgaaccggcg
     1921 agcacgatgt cgccgcgctt caaggtgccg ctttgcacca gcaccgtggc gaccggtccg
     1981 cggcccttgt cgagctgggc ctcgatgacg agtcccttgg ccatcgcatc cttgggggcc
     2041 ttcagctcga gcacttcggc ctgcaggagc acctgctcga gcagctgatc caccccctgg
     2101 cccgtcttgg ccgacaccgc gacgaatggc ga
//
LOCUS       DASRWS010000684         5511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1989971, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000684 DASRWS010000000
VERSION     DASRWS010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989971"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..494)
                     /locus_tag="VFF72_13060"
     CDS             complement(24..494)
                     /locus_tag="VFF72_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZV94142.1"
                     /translation="MTQTRSQDSNALHAVVRDPGLDDREFQMLAEFRFALRRYLRFSE
                     IEAEKAGLTAQQYQALLAVRANAAIGEMTISELARQLLVKHHSAVGLVDRLVGQELLE
                     RAAASDSRRKVALRLTAKGKRVLNGLASTHRQELRDASHELARVLQRMSRVMPH"
     gene            711..1394
                     /locus_tag="VFF72_13065"
     CDS             711..1394
                     /locus_tag="VFF72_13065"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZV94143.1"
                     /translation="MTRDVAWVTPEAPVLDVAELLVARRVSAVPVLDNGELVGIVSEV
                     DLLHRQELGTAWDPFDRPWWVRLFKGEQWPASYVKSHAATVADVMSPPVITVLEEASM
                     VELAKVLERHSIRWVPVLRGRTLVGIVSRSDLIRALVAKGRMARGPGCTNDDAIHTAV
                     LGELEVQPWWRPGQSQVTVSDGVVHFSGLVDSHDEARAARVAAENIPGVKGVQDHRLD
                     GVMPALGYW"
     gene            complement(1419..2363)
                     /locus_tag="VFF72_13070"
     CDS             complement(1419..2363)
                     /locus_tag="VFF72_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZV94144.1"
                     /translation="MLDLNDMVTFARVVEAGSFSAAARRLGTSKSGASKTISRLEKDL
                     GSRLLNRSTRKLGLTGIGTVFYEHCARIVDEAMHAADSVSRLQSDPSGVLRITAPVAF
                     GRLHVAPAVVEYLARYPMVKIDMSVTDRMVDLVEEGFDLAIRITREPGPQLVARDLAP
                     IRRVVCATPGYLGRFGVPQTPFDLERHNCLDYSRFGTQGRWRLQGADGEIVVDVSGSL
                     RIDDDDTLAQAVIAGLGIAMLPTFIVGRELQAGRLQSMLDDYTPIERRVYSVRLPNVR
                     PPVKVRGFIDFLLARFGEVPYWDRADDAWPAALESKAA"
     gene            2501..2926
                     /locus_tag="VFF72_13075"
     CDS             2501..2926
                     /locus_tag="VFF72_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94145.1"
                     /translation="MTHLSESIRVPTLIWSTTTSTAPAQSEEGMEKKDLQPPWNDSFR
                     LPGWLGMAWFCYGWWERESLLTLEAMGMPLAPASARPMPATREGSGARDTRPGKGDDS
                     GADTTVQSQPPAALRAASKHRRTTRAAAHRRSHAGYAVS"
     gene            2936..3703
                     /locus_tag="VFF72_13080"
     CDS             2936..3703
                     /locus_tag="VFF72_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZV94146.1"
                     /translation="MLDANRRSGNLLLDGLAGAARERLEPDLVPVQMPAGQVLHFAQA
                     PVREAYFPTTSVVSKLFLMRNGASSGVALVGAEGMIGVAAFLGGSALPDQAQVLRAGE
                     GWKISAQRLADEFERGGPLMHALLGYTHSLIAQVMQTAACNSHGTLQERLCRWLLMNL
                     DRGCNEEMKMTHQGIADVLGVRRESVSETAAILQHEGAIRYRWGHVRVLDRRALERRV
                     CECYRPVAPRQVAVASRRPIGPVSSAFGRRAIASADA"
     gene            complement(3785..4393)
                     /locus_tag="VFF72_13085"
     CDS             complement(3785..4393)
                     /locus_tag="VFF72_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZV94147.1"
                     /translation="MALPRVDPPTRPASRRGGVRSSSGESEDACRVLTVGHSTRPIEE
                     FIDLLVAHDVTQLVDVRTVPRSRHNPQFGIEALPASLAHAGIGYAHAPGLGGFRHAVQ
                     DSPNSGWRNLSFRGYADYMQTADFSAELTGLIELAKEERAVLMCAEAVPWRCHRSLIA
                     DALLVHGIASCEIVSARRLQVHRLTSFARVRGLDLTYPPEGE"
     gene            4583..5347
                     /locus_tag="VFF72_13090"
     CDS             4583..5347
                     /locus_tag="VFF72_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZV94148.1"
                     /translation="MKLHVLSDLHLGFSDMAVPQGDADLVVLAGDIARPREALAWARR
                     FDKPVLYVAGNHEFYGGSLDGVAAELETLSAGSCVRVLDNAETVIGDVRFLGTTLWTD
                     FELFGQGDKRLAAMAEAQRLLRDFSRIRCAEAGERLFSPDDSAQLFGRNASWLDARLA
                     SPHDGPTVVITHHAPSPRSIHPRFAQSLLNACFVSDAEYLLDGRRAALWIHGHTHDSF
                     DYEVNGTRVVCNPRGYAKAGVDENPHFDPSLTIEVG"
BASE COUNT          899 a         1821 c         1893 g          898 t
ORIGIN      
        1 gcgatgcacg atcgccgaag acctcaatgc ggcatgacgc gagacatccg ctgcagcacc
       61 cgggccagct catgcgacgc gtcgcgcaat tcctggcgat gggtgctggc caggccgttg
      121 agcacgcgct ttcccttggc ggtgagtcgc agggcgacct tgcgccggct gtcgctcgcg
      181 gcggcgcgtt cgagcagctc ctggccgacg agccggtcga cgagtccgac ggcgctgtga
      241 tgcttgacga gcagttgtcg cgccagttcg ctgatcgtca tctcgccgat cgcggcgttg
      301 gctcgcacgg ccagcagcgc ctggtactgc tgcgcggtca agccggcctt ctcggcctcg
      361 atctcgctga agcgtaggta ccggcgcagc gcgaagcgga actccgccag catctggaat
      421 tcgcgatcgt ccagccccgg gtcgcgcacc acggcgtgca acgcgttgga atcctgggaa
      481 cgggtctggg tcatgaaagg aggttcgatc ccgagatgtt gcgaaaccgc aaggcgcggg
      541 ccttgacaag gcatcgtaat acgacgataa cattatcgca gtgcgataaa catgggacgg
      601 aggttcgccg cgatggtcgt ggccggcgcg ccttatgcaa ggagggtcca cgaccgctcg
      661 cgccggctct gccgacgccc gtggaggccg caatgcttgc aagagacgtc atgacccgcg
      721 acgtggcctg ggtcaccccg gaggctccgg tgctcgacgt cgccgagctt ctcgttgcca
      781 ggcgtgtcag cgccgtgccc gtgctcgaca atggcgagct cgtcggcatc gtcagcgagg
      841 tcgatctcct gcatcgccag gagctcggca cggcctggga tcctttcgac cggccgtggt
      901 gggtgcgcct cttcaagggc gagcagtggc cggcgagcta cgtgaagtcg catgccgcga
      961 cggttgccga cgtgatgtca ccaccggtca tcaccgtcct cgaggaggcg tcgatggtcg
     1021 agttggcgaa ggttctcgag aggcattcga tacgttgggt gcccgtcctg cgcggccgga
     1081 cgctcgtggg catcgtcagt cgctcggacc tgatccgggc cctggttgcc aaggggcgca
     1141 tggcacgcgg ccctggctgc accaacgacg atgccatcca caccgcggtg ctgggcgagc
     1201 tcgaggtgca accctggtgg cggccgggac agtcgcaggt caccgtgtcc gatggcgtcg
     1261 tgcatttttc cgggcttgtc gattcgcacg atgaggcacg ggcggcgcgc gtggctgccg
     1321 agaacatccc gggcgtcaaa ggggtccagg atcaccgtct cgacggggtg atgccggccc
     1381 tgggctactg gtaggccgag ccggcagccg cgaggtcatc aggccgcctt cgattcgagg
     1441 gcggcgggcc aggcatcgtc ggcccgatcc cagtaaggca cctcgccgaa gcgggccaga
     1501 aggaaatcga tgaacccgcg caccttgacc ggcgggcgca cattgggcag ccgtaccgag
     1561 tagacgcgcc gctcgatcgg ggtgtagtcg tcaagcatcg actggaggcg tccggcctgc
     1621 aattcccggc cgacgatgaa ggtcggcaac atggcgatgc ccagtccggc gatcacggcc
     1681 tgggccagcg tgtcgtcgtc gtcgatgcgc agcgacccgc tcacgtcgac gacgatctcg
     1741 ccgtcggcgc cttgcagccg ccaccgccct tgcgtgccga agcggctgta gtcgagacag
     1801 ttgtgccgtt cgaggtcgaa aggagtctgc gggacgccga agcggccgag gtacccgggc
     1861 gtggcgcaca cgacccgccg gatgggtgcc aggtcgcgtg cgacgagttg cgggccgggc
     1921 tcgcgcgtga tgcggatggc gaggtcgaat ccttcctcga ccagatcgac cattcggtcg
     1981 gtcaccgaca tgtcgatctt caccatcgga tagcgtgcca ggtactcgac caccgccgga
     2041 gcgacgtgaa gccggccgaa cgccaccggc gcggtgatcc gcaatacgcc gctcgggtcg
     2101 gattgcaggc ggctcacgct gtcggccgcg tgcatcgcct cgtcgacgat tcgcgcgcaa
     2161 tgttcgtaga acacggtgcc gatgcccgtc aggcccagct tgcgcgtgct gcgattgagc
     2221 aatcggctgc ctaggtcctt ctcgaggcgc gagatcgtct tgctggcccc ggacttcgac
     2281 gtgccgagcc ggcgcgccgc tgccgaaaag cttccggcct cgacgacacg agcgaacgtc
     2341 accatgtcgt tgaggtcgag catttgccac tcctcgggtc gacagcgcgc atcgcccggt
     2401 gtgcggccgt gacggagatt ctggcgcagc gaccgcgctg gcgtcgaccg gaccggatcg
     2461 cgcccgcgcc tgtcgcctgc gagaaacagt ctggttcgcc gtgacgcact tgtccgaaag
     2521 catccgcgtg cctacattga tctggtcgac gaccacgtcg actgcgccag cacaaagcga
     2581 ggaaggcatg gaaaagaagg atcttcagcc gccgtggaac gactcctttc gcctccccgg
     2641 atggctcggc atggcgtggt tctgctacgg atggtgggaa cgcgagagtc tgctcaccct
     2701 ggaggcgatg ggcatgcccc tggcgcccgc gtccgcccga ccgatgccgg cgacccgcga
     2761 ggggtccggc gcgcgtgata cccggccggg caagggcgac gattcgggcg ccgacacgac
     2821 cgtgcaatcg caacctccgg cggctcttcg agcggcatcg aagcatcggc gaacgacacg
     2881 tgcagcagcg caccgcaggt cgcatgccgg ctacgcggtg tcataggagc cacccatgct
     2941 cgacgccaat cgacgctcag gcaacctcct tctcgacgga cttgccggcg ccgcgcgcga
     3001 gcgcctggag cccgatctgg tccccgtcca gatgcctgcc ggacaggtcc tgcatttcgc
     3061 gcaggctcct gtacgggagg cctatttccc gacgacatca gtcgtctcca agctgttcct
     3121 gatgcgcaat ggcgcctcgt cgggggtcgc gctggtcggt gccgaaggca tgatcggcgt
     3181 ggccgcgttt ctggggggat ccgcgctgcc cgatcaggcg caggtgcttc gcgcgggcga
     3241 gggctggaag atctcggcac aacgcctcgc cgatgaattc gaacgtggcg ggccgctcat
     3301 gcatgcgctg ctcggctaca cgcactcgct catcgcccag gtgatgcaga cggcggcttg
     3361 caacagccat ggcaccttgc aggaacggct ttgccgctgg ctgctcatga acctcgatcg
     3421 cggctgcaat gaagaaatga agatgacgca ccagggcatc gccgatgttc tcggcgttcg
     3481 gcgagaaagc gtcagcgaga cggcagccat tcttcaacac gaaggggcga ttcggtatcg
     3541 ctggggtcat gtgcgcgtgc tcgatcgacg ggcactcgag cgccgggtct gcgagtgcta
     3601 ccgaccggtc gcgccaaggc aagtggccgt cgcgtcgcgt cggccgatcg gcccggtgtc
     3661 gtcggcgttc ggccgccgcg cgatcgccag cgcagacgcg tagctcgaat cagcccgcga
     3721 cctgcaggcg ggcgagctcc agcgaatcgt ctttccggcg tgcgtgggaa tcgaccaggc
     3781 gcaatcactc gccctcgggt gggtaggtca ggtcgagccc gcgaacgcgg gcgaaggatg
     3841 tcaggcgatg gacctgcaaa cgccgggcgc tgacgatctc gcagctggct atgccgtgca
     3901 cgagcaacgc gtcggcgatc agcgaacgat ggcaccgcca gggcacggcc tcggcgcaca
     3961 tcagcaccgc ccgttcctcc tttgcgagct cgatcaggcc ggtgagctcg gccgagaaat
     4021 cggctgtctg catgtagtcg gcataacccc ggaacgagag gttgcgccag ccagagttgg
     4081 gcgaatcctg caccgcgtgg cgaaagccgc cgaggcccgg cgcatgggcg tagccgatgc
     4141 cggcgtgcgc cagcgacgcc ggcaatgcct cgatgccgaa ctgcgggttg tgccgtgagc
     4201 gcggcacggt gcggacatcg acgagctgcg tcacgtcgtg agcgacgagc aggtcgatga
     4261 actcctcgat cggtcgggtg gagtggccga ccgtgagaac ccggcatgcg tcttcgcttt
     4321 cgcccgagct cgaccgaacg ccgccgcgcc gagatgccgg tcgagtcggg ggatcgacgc
     4381 gaggaagggc catgtcaggt gaggcaatgc gcctgcgcgc agcggccgac cagcagcgtc
     4441 agcacgatcg gcacgccgcc cggcaacgcc ggttcgggtc gacgaggaat gggcgtggaa
     4501 atcgtcgaag ccatgacgcg ccaatatatc gcggtgtgat atatctttcc cgctcgatgg
     4561 ccttccggga gcgcgcgccg cgatgaagct tcatgtcctg tcggatctgc acctgggctt
     4621 cagcgacatg gcggtgccgc aaggcgacgc cgacctggtc gtcctggccg gcgacatcgc
     4681 ccgcccgcgg gaggcgctgg cctgggcccg tcgcttcgac aagccggtgc tctatgtcgc
     4741 cggcaatcac gagttctatg gcggcagcct cgacggcgtc gccgcggaac tcgagacgct
     4801 gagtgcgggc agctgcgttc gggtcctcga caacgccgag acggtcatcg gcgatgtccg
     4861 gtttctgggc accaccttgt ggaccgactt cgagctgttc ggtcagggtg acaagcggct
     4921 ggcggccatg gccgaagcgc agcgcctgct ccgcgacttc agcaggattc gctgcgccga
     4981 ggcgggcgag cgcctgttct cgccggacga ctcggcgcaa ctcttcggcc gcaacgccag
     5041 ctggctcgac gccaggctcg catcgccgca cgacgggccg accgtcgtca tcacccacca
     5101 cgcgccatcg ccgcgcagca tccatccccg cttcgcgcag tcgctgctca atgcctgctt
     5161 cgtctccgac gccgaatacc ttctcgatgg acgccgcgcc gcgctgtgga tacacggcca
     5221 cacccacgac agcttcgact acgaggtcaa cggaacccgc gtcgtctgca acccgcgcgg
     5281 ctatgcgaag gccggggtcg acgagaaccc gcacttcgat ccgtcgctga cgatcgaggt
     5341 gggctagggc acccgacggc aacgccgcgg caagcggcga tcgcttgcgt gcatatcatc
     5401 gcgggtgcct tgcatcgacg aagctccgtc ccgactgccg cggcctcatg acacgagtcg
     5461 cctgcttcat cgcgctgcga tgaacgaaga gccccggagt ccggggcgcg c
//
LOCUS       DASRWS010000685         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_854320, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000685 DASRWS010000000
VERSION     DASRWS010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854320"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFF72_13095"
     CDS             complement(<1..1145)
                     /locus_tag="VFF72_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZV94149.1"
                     /translation="MMPLPGWIAGLAMVVIGGCLLSHPACAATPAAPAHDDAIAWRHA
                     ANDADVEAAFDAARAAHKPVFVYWGAVWCPPCNQVKSTIFNREDFIARSRAFVPVYID
                     GDSPGAQKLGARFHVSGYPTMLLFRPDGQEVMRLPGEVDARRYAEVLDLGIDAARPVK
                     ALLADALAGRRLAPGDWRLLAFYSYDTDEHQLLPAKQVPATLARLADACPTDLPATRI
                     RLRLKALAAAGETHTRLGGARTRAALLSLLGDPAAARQQADLIVNWGPEIVRAASSGE
                     PVPRARLVVATDLALKRLQADASLSRADRMQALASRVELARLDRPDGPAPAMPATLLA
                     EAREASARADREISDGYERQAVITAAAYMLERAGLSRDSDALLEGNLA"
     gene            complement(1235..1975)
                     /locus_tag="VFF72_13100"
     CDS             complement(1235..1975)
                     /locus_tag="VFF72_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94150.1"
                     /translation="MSPGLSPSVVVAIGVPHRRRLREVYRSAGWPCRDSIEIELVAAG
                     LLERERGPLGHETLRVSDAGVAVLAETLVKNRARRDGHEMLVERVAREMTRAGRLAWR
                     GLALRARVGDAWAMAMPDVFSVRHTTVEDYLEPVVHEVKFHRSDLLADLRRSNKREAY
                     LQIGGECWYVLREGIAEASEIPSECGVLVATGERLEVARPAPRRPFRLPFGTWMALAR
                     ATPIEGWRLDDSQGSLGGERAPPGSAAP"
     gene            2137..3210
                     /locus_tag="VFF72_13105"
     CDS             2137..3210
                     /locus_tag="VFF72_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZV94151.1"
                     /translation="MALLGTAGAHAADAKVLNVYNWSDYIADDTIKNFEKETGIKVNY
                     DNYDSNEVLLAKLVAGNTGYDIVVPSSHFAKLMIEGGLLQKLDRAKLTNWGNLDPALL
                     KHLQTVDPGNQYLVDWLWGYVTVGINIPKVKAALGGMPMPENAWSLIFDPKYASKLKG
                     CGINFLDSASEVLPVAMLYVGKPAYSKNAADYDAAAKMLQAVRPYVTRFSSSGYINDL
                     AGGSLCAVMGYSGDINIARARALDADPKKPAAIEALIPKGGATLFFDTMAIPKDAKNV
                     ANAHLFINYILRPEVAASLTNKVFYANPNIASLKYVKKSVADNPTIFLSASDKARMTA
                     PDAVPQPIRRVQTRIFTNFKAGR"
     gene            complement(3222..3500)
                     /locus_tag="VFF72_13110"
     CDS             complement(3222..3500)
                     /locus_tag="VFF72_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZV94152.1"
                     /translation="MKATWNGATLAESDDTVVVEGNHYFPEASLKKEYTTFSNHRSMC
                     PWKGEAHYYSLMVDGDLNPNAVWYYPDPKEAAKEIKGRVAFWKGVVVE"
     gene            3668..>4649
                     /locus_tag="VFF72_13115"
     CDS             3668..>4649
                     /locus_tag="VFF72_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZV94153.1"
                     /translation="MTTKVRKGQAPGQIDRGEFGIRFRNAFVDPAYRAEDDAIARLEA
                     IAWDAFRDGRKAPVTRKAGAGYADPSYDLSVDWLQTRDRLAQAQKAWADPATKTRALV
                     ICASPRNDGTCPGEISKTFRLAGLVNEVLVEENVDVDFLDLSLVSSEYGRKIHPCKGC
                     VSTAMPLCHWPCSCYPNHSLGQTNDWMAEIYERWVAAHAVVIVTPVHWYQSPSALKLM
                     IDRLVCADGGNPDPTSTHGKKPAEAKALELAGWDYPKHLDKRVYGLVVHGDVAGIEGS
                     RRALSDWLDWMGLLDAGDQARLDRFIGYYEPYATSHDALDKDDAMQQETRN"
BASE COUNT          790 a         1641 c         1526 g          692 t
ORIGIN      
        1 gccaggttgc cctccagaag cgcgtcggaa tcgcgcgaca gccccgccct ctcgagcatg
       61 taggcggccg cggtgatcac ggcctggcgc tcgtagccgt cgctgatctc gcggtcggcg
      121 cgggccgatg cctcccgggc ctcggcgagc agtgtcgccg gcatcgccgg cgccgggccg
      181 tcgggacgat cgagtctcgc cagctcgacg cgactggcca gcgcctgcat ccgatcggcc
      241 cgcgacagcg acgcgtcggc ctgcagccgc ttcagcgcca gatcggtcgc gacgacgagc
      301 cgtgcccttg gcaccggctc gccggacgac gcggcacgaa cgatctccgg cccccagttg
      361 acgatcaggt cggcctgctg gcgcgccgcc gcgggatcgc ccagcagcga caacagggcg
      421 gcacgggtgc gcgcacctcc caggcgcgtg tgcgtctcgc cggcggcggc gagcgctttc
      481 aggcgcagtc gaatcctggt cgccggaagg tccgtcgggc aggcatcggc caggcgcgcc
      541 agcgtcgcgg gcacctgctt cgcgggcaac agctggtgct cgtcggtgtc gtacgaatag
      601 aaggcgagca gccgccagtc gcccggcgcc aggcgccgtc cggcgagggc gtctgcgagg
      661 agcgccttca ccggccgcgc cgcgtcgatg cccagatcga gcacctcggc gtagcgccgg
      721 gcgtccacct cgcccggcag gcgcatcacc tcctgcccgt ccggcctgaa cagcagcatc
      781 gtcggatagc cgctgacgtg aaagcgggcg ccgagctttt gcgcgcccgg gctgtcgccg
      841 tcgatgtaga ccggcacgaa ggcacgtgag cgggcgatga aatcctctcg gttgaagatc
      901 gtcgacttca cctggttgca cggcgggcac cacaccgcgc cccaatagac gaagaccggc
      961 ttgtgcgcgg cacgcgcggc gtcgaacgcc gcttcgacgt cggcatcgtt ggccgcatgt
     1021 cgccaggcga tcgcgtcgtc gtgcgctggc gccgccgggg tcgccgcgca ggccgggtgc
     1081 gaaaggaggc acccgccgat cacgaccatc gccagcccgg cgatccagcc cggcagaggc
     1141 atcatccgcg gcgcgcgcgc ggtccgagtg ggggagacac gcatacgctt gccatcatcg
     1201 cccaatcgat cgaccggcgc cggcggccgc cacgtcatgg ggccgcgctg cccggcgggg
     1261 ctcgctcgcc gccgaggctg ccttgcgaat catcgaggcg ccagccctcg atcggcgtcg
     1321 cccgcgccag cgccatccag gtcccgaaag gcagcctgaa cgggcgcctg ggcgcgggcc
     1381 tggcgacttc gaggcgttcg cccgtcgcga cgaggacgcc gcattcggac ggaatctcgg
     1441 acgcctcggc gatgccctcg cgcagcacgt accagcattc gccgccgatc tgcaggtagg
     1501 cctcgcgctt gttgctgcgg cgcagatcgg caagcaggtc ggagcgatgg aacttcacct
     1561 cgtggacgac cggctcgaga tagtcctcga ccgtggtgtg ccgaacggaa aatacgtcgg
     1621 gcatggccat cgcccaggcg tcgccgaccc gggcgcgcaa cgccagcccg cgccaggcga
     1681 gccggccggc acgagtcatc tcgcgggcga cccgctcgac cagcatctcg tgaccgtccc
     1741 ggcgggctcg gtttttcact agcgtctcgg cgagcacggc gacgccagcg tcgctgacgc
     1801 gcagcgtctc gtggccgagc ggacctcgct cacgctcgag cagcccggcg gcgacgagct
     1861 cgatctcgat cgaatcgcga cacggccaac cggcggagcg atagacctcg cgcaggcggc
     1921 gccggtgcgg cacgccgatc gcgacgacga cggagggcga aagacctgga gacatggcgg
     1981 gccgttgcga gtcttgcacc ataaccaact ttcgatcgcc cggcgacatc gacgctagga
     2041 ttcggtccct cgccttcgat gccgcccaca cctggaaccg ccgccatgcc gccacgcctg
     2101 cttttccgcc tgcgccgcct cgcctcgatc gccgcgatgg ccctgctcgg cactgccggc
     2161 gcacacgcgg ccgacgcgaa ggtgctcaac gtctacaact ggtccgacta catcgccgac
     2221 gacacgatca agaatttcga gaaagaaacc ggcatcaagg tcaactacga caactacgac
     2281 agcaacgagg tgctgctcgc caagctggtg gccggcaata ccggctacga catcgtcgtg
     2341 ccgagctcgc atttcgccaa gctgatgatc gaaggcggcc tgttgcaaaa gctcgaccgc
     2401 gccaagctca ccaactgggg caacctggac ccggcgcttc tcaagcacct gcagaccgtc
     2461 gaccccggaa accagtatct ggtcgactgg ctgtggggct acgtcacggt cggcatcaac
     2521 atcccgaagg tgaaggcggc gctcggcggc atgccgatgc ccgaaaacgc ctggagcctg
     2581 atcttcgatc cgaaatacgc ttcgaagctg aagggttgcg gcatcaattt cctcgactcg
     2641 gcttccgagg tgctgccggt ggcaatgctc tacgtcggca agcccgccta tagcaagaac
     2701 gcggccgact acgacgcggc ggcgaagatg ctgcaggcgg tgcggcccta cgtgacacgc
     2761 ttctcgtcgt cgggctacat caacgacctg gccggcgggt cgctgtgcgc cgtgatgggc
     2821 tactcgggcg acatcaacat cgcccgcgcc agggccctcg acgccgatcc gaagaagccg
     2881 gcggcgatcg aggcgttgat cccgaaaggc ggcgcgacgc tcttcttcga cacgatggcg
     2941 atcccgaagg acgcgaagaa cgttgccaac gcgcacctct tcatcaacta catccttcgc
     3001 cccgaggtgg ccgcatcgct caccaacaag gtcttctacg ccaacccgaa catcgcctcg
     3061 ctgaaatacg tgaagaagtc ggtggccgac aacccgacca tcttcctcag tgcgagcgac
     3121 aaggcgcgca tgaccgcacc cgatgccgtc ccgcagccga tccggcgcgt gcagacgcgc
     3181 atcttcacca acttcaaggc cggccgatag gccggcgcag atcactcgac gacgacgcct
     3241 ttccagaagg cgacgcgccc cttgatttcc ttggccgcct ccttcggatc ggggtagtac
     3301 cagacggcgt tgggattgag gtcgccgtcg accatgaggc tgtagtagtg cgcctcgccc
     3361 ttccacgggc acatcgagcg gtgattgctg aaggtggtgt attccttctt caggctggct
     3421 tcgggaaagt agtggttgcc ctcgacgacg acggtgtcgt cgctttcggc gagggtcgcg
     3481 ccgttccagg ttgccttcat gtccttgcct cgcgttgtcg gatcgagccg cacgccgcgg
     3541 catgatgcag atcgtagcga tcgcgccgcg gttgtgcctg cggacgcggc attggccgac
     3601 acgacgcgtc aaacggcgcc gctagagtcg tccgacatgt cgcgcggcgg caagggaggt
     3661 ttccaggatg acgaccaagg tgcgcaaggg tcaggcaccc gggcagatcg atcgcggcga
     3721 attcggcatc cggtttcgca acgccttcgt cgacccggcg tatcgcgcgg aagacgacgc
     3781 gatcgcgcgc ctcgaagcga tcgcctggga cgcttttcgc gacggccgca aggcgccggt
     3841 cacgcgcaag gcgggcgccg gctacgccga cccctcgtac gacctctcgg tcgactggct
     3901 gcagacgcgc gaccggctcg cgcaggcgca gaaggcgtgg gccgacccgg cgacgaagac
     3961 acgcgcgctc gtcatctgcg cctcgccacg caacgacggt acctgccccg gcgagatctc
     4021 gaagaccttc cgtctggccg ggctcgtcaa cgaggtgctg gtcgaggaaa acgtcgacgt
     4081 cgacttcctc gacctgagcc tcgtgtcttc cgaatacggt cgaaagatcc acccctgcaa
     4141 gggctgcgtc tcgacggcga tgccgctctg ccactggccc tgcagctgct atccgaacca
     4201 ctcgctcggc cagaccaacg actggatggc cgagatctac gagcgctggg tcgccgcgca
     4261 cgccgtcgtc atcgtcacgc cggtgcactg gtaccagagc ccgagcgcgc tcaagctgat
     4321 gatcgatcgc ctcgtctgcg ccgacggcgg caaccccgac ccgacatcga cgcacggcaa
     4381 gaaaccggcc gaggcgaagg cgctcgagct ggctggctgg gactatccga agcatctcga
     4441 caaacgcgtc tacgggctgg tcgtgcacgg cgacgtcgcc ggcatcgaag ggtcgcgccg
     4501 cgccctctcg gactggctcg actggatggg cctcctcgac gccggcgacc aggcgcgtct
     4561 cgatcgcttc atcggctact acgaacccta cgcgaccagc cacgacgcgc tcgacaagga
     4621 cgacgcgatg cagcaggaga cccgaaacg
//
LOCUS       DASRWS010000686         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4009145, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000686 DASRWS010000000
VERSION     DASRWS010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4009145"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFF72_13120"
     CDS             complement(<1..269)
                     /locus_tag="VFF72_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HZV94154.1"
                     /translation="MSEQELKLHVPSAHAAAIARELLDHGATRVRLRAIYFDTPEREL
                     ARARVALRLRLEGRRWMQTLKTPGADAITRVELNHPRPGPTLDLSV"
     gene            471..1637
                     /gene="ribBA"
                     /locus_tag="VFF72_13125"
     CDS             471..1637
                     /gene="ribBA"
                     /locus_tag="VFF72_13125"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZV94155.1"
                     /translation="MSVHPLNTPDSAADDFQIASVPEIIAELRAGRIVILVDEEDREN
                     EGDLVMAAEFVTPDAINFMVTHGRGLVCLTLTEERVRQLDLPMMTSRNGTRYGTNFTQ
                     SIEAAEGVETGISAADRARTIQVAVARNAKPSDLVQPGHVFPLRAVSGGVLVRAGHTE
                     AGCDLTRMAGLTPAAVICEILKPDGTMARLPDLMVFAREHGLKIGTIASLIQYRSEHE
                     SIVQRVGERVMQTPWGEFRAVAYRDDASGSPHLALVHGDIDPQRETLVRVHEPASLLD
                     VLDAGQGAHSWTVAQALSAIAASPAGVMVLMNCQSSTEHLFNQIGGWTDPAAVPPPSD
                     GERFGLRTYGIGAQILRDLNVGRMRLLARERKMPSMAGFSLAITGYDCPPSANP"
     gene            1654..2166
                     /gene="ribH"
                     /locus_tag="VFF72_13130"
     CDS             1654..2166
                     /gene="ribH"
                     /locus_tag="VFF72_13130"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZV94156.1"
                     /translation="MNPYVLTPDLNGEGLHIGIVRARFNEEIGQAELEACLEELGKLG
                     VDERDVMIASVPGALELGVALSHMAETFEFDALIALGAVIRGETYHFEVVSNEMATAI
                     NRISLETGIPVANGVLTVDTDEQAQARAAGKGRDCAQVAVEMANLVAALEPEEEDDED
                     EDFDDEEDER"
     gene            2141..>2529
                     /locus_tag="VFF72_13135"
     CDS             2141..>2529
                     /locus_tag="VFF72_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HZV94157.1"
                     /translation="MTTKKTSAETPAQGRGNARSARRRAREFALQGVYAWLLRGGEGT
                     QDAGEIDAHLRDAEDFSEADAQWFKTLLHGVLQEAPGLRERFTPYVDRPLAELSPVEH
                     GILLIGSYELVYHVEVPYKVAINEAVEL"
BASE COUNT          435 a          863 c          830 g          401 t
ORIGIN      
        1 accgagaggt cgagcgtcgg gcccggacgg gggtggttga gctccacccg cgtgattgcg
       61 tcggcgccgg gcgtcttgag cgtctgcatc caccggcggc cttccaggcg cagccgcagt
      121 gccacgcggg cgcgcgccaa ctcgcgttcg ggcgtgtcga aatagatggc gcgcagcctg
      181 acgcgggtgg cgccgtggtc caggagttcg cgcgcaatcg cggcggcgtg ggccgacggc
      241 acgtggagtt tcaattcttg ttctgacatg tgccgaagcg atccgcagtc gggaagagcc
      301 aaacatctta cgggcgagac ttatggaatt tgatgacgag tcataaattg ttcgcaattg
      361 ccgccggacc gcgcgggatg cactgttcca ccggttgggg caggcaagcc cctgaatacg
      421 ggccgggggg cggtaaaatg gcgctttgcc gcttttcgca ggctccttcc atgtccgttc
      481 atcccctcaa cacccccgat tcggccgccg acgatttcca gatcgcatcg gtccccgaga
      541 tcatcgccga gctgcgcgcc ggccgcatcg tcatcctggt cgacgaagaa gaccgcgaga
      601 acgaaggcga tctggtcatg gccgccgaat tcgtcactcc cgacgccatc aacttcatgg
      661 tcacccacgg ccgtggtctg gtgtgcctga cgctcaccga agagcgcgtg cggcagctcg
      721 acctgcccat gatgaccagc cgcaacggca cgcgctacgg caccaacttc acgcagtcca
      781 tcgaggcggc cgaaggcgtc gagaccggca tctccgccgc cgaccgcgcg cgcaccatcc
      841 aggtagccgt ggcgcgcaac gccaagccgt ccgacctggt gcagcccggc catgtcttcc
      901 cgctgcgcgc ggtgtccggc ggcgtgctgg tgcgcgccgg ccacaccgaa gccggctgcg
      961 acctgacccg catggcgggc ctgacgccgg cggcagtcat ctgcgagatc ctcaagcccg
     1021 acggcaccat ggcgcgcctg cccgacctga tggtcttcgc gcgcgagcat ggcctgaaga
     1081 tcggcaccat cgcctcgctc atccagtacc gcagcgagca cgagtccatc gtgcagcgcg
     1141 tgggcgagcg cgtcatgcag acgccctggg gcgagttccg cgccgtggcc taccgcgacg
     1201 acgcctccgg ctcgccgcat ctggcgctgg tgcacggcga catcgatccg cagcgcgaaa
     1261 ccctggtgcg cgtgcacgaa cccgcctcgc tgctcgacgt gctggacgcg gggcagggcg
     1321 cgcacagctg gacggtcgcc caggcgctga gtgccatcgc cgcatcgccg gcgggtgtta
     1381 tggtgctgat gaactgccag tcgtccaccg aacacctttt caaccagatc ggcggctgga
     1441 ccgatccggc cgcggtgccg ccgccttccg acggcgagcg tttcggcctg cgcacctacg
     1501 gcatcggcgc gcaaatcctg cgcgacctga atgtcggccg catgcgcctt ctggcgcgcg
     1561 aacgcaagat gcccagcatg gcgggcttct cgctcgccat tacgggttac gattgccctc
     1621 cctccgccaa cccctgaaac aaggcctgaa cccatgaacc cctacgttct caccccggat
     1681 ctcaacggcg aaggcctgca catcggcatc gtccgtgccc gtttcaacga agaaatcggc
     1741 caagccgagc tggaagcctg cctcgaagaa ctgggcaagc tgggcgtgga cgagcgcgac
     1801 gtcatgatcg ccagcgtccc cggcgcgctc gagctgggcg tggccctctc gcacatggcc
     1861 gagactttcg agttcgacgc cttgatcgcg ctgggcgcgg tgatccgcgg cgaaacctac
     1921 catttcgagg tggtcagcaa tgaaatggcc accgccatca accgcatttc gctggaaacc
     1981 ggcattccgg tggccaacgg cgtgctgacg gtcgataccg acgagcaggc ccaggcgcgc
     2041 gccgcgggca agggccgcga ctgcgcccaa gtggccgtgg aaatggccaa cctggtcgcc
     2101 gcgctcgaac ccgaagaaga agacgacgag gacgaagatt ttgacgacga agaagacgag
     2161 cgctgagacg cccgcccagg gccgcggcaa cgcccgcagc gcgcgccgcc gtgcgcgcga
     2221 gttcgcgctg cagggcgtgt acgcgtggct gttgcgcggc ggcgaaggca cgcaggacgc
     2281 cggcgagatc gacgcgcatc tgcgcgatgc cgaagatttc tccgaagccg acgcgcagtg
     2341 gttcaagacc ctgctgcatg gcgtgctgca agaggcaccc ggtttgcgcg agcgcttcac
     2401 gccgtacgtc gatcgtccgc tggccgagct ttctcctgtg gaacacggca tcttgctgat
     2461 cggcagctac gagctggtgt atcacgtcga ggtgccttac aaggtggcca tcaacgaggc
     2521 ggtggaact
//
LOCUS       DASRWS010000687         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3730340, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000687 DASRWS010000000
VERSION     DASRWS010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3730340"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /gene="ggt"
                     /locus_tag="VFF72_13140"
     CDS             complement(<1..1209)
                     /gene="ggt"
                     /locus_tag="VFF72_13140"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZV94158.1"
                     /translation="MSPTISCRLVSRCLAVVVALSAAAGGGAGAASVAPVMAEHGMVV
                     TAQHLATEVGVDILKKGGNAVDAAVAVGYALAVVYPAAGNLGGGGFMTIQLADGRRTF
                     LDFREKAPLAASADMYLDAAGDVVKGSTRRGYLAAGVPGSVSGLETARTRYGTMSRAE
                     LIAPAIAFAERGFVLDEGDAEMFATATAAFEADPPSRAIFLHEDGRPFAAGDRLVQHD
                     LAQTLRRIAESGTAGFYEGPVGAAIVASSRAGKGLLTQADLDAYTTRERAPVECDYRG
                     YHVVSAPPPSSGGVILCELLDILEGYPLTSWGFRSARGVHVQIEAMRHAYVDRNSELG
                     DPDFVANPIARLLDKAYAAKIRAVIDPDRAADSKSLGPGIAPHEGSNTTHYSIVDGQG
                     NAVAVTYTLND"
     gene            1480..2658
                     /locus_tag="VFF72_13145"
     CDS             1480..2658
                     /locus_tag="VFF72_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94159.1"
                     /translation="MRRVPPLVRALAFAACVGPMLAACSPLSLGLTAAGVATDSSMTW
                     AIVKHVHAKLTEDDPTPCILLNSVQRALNARCDYHPGSIRAADFASSGLQECPLAAAT
                     RDPRLWRALPELIEKGPGVDKCNGSPLLALAESDPCPDFAAASPAVRQSLATLAESDP
                     RAVRHDVFRMFSCSNARAVGLDHVLVTWLDHGELEPGKLSFSPLSALDPDMLVGRFGH
                     ELEVAGHTPEAALDGYEGTLPSGYETALRRSDWAALEWWLYRLPQLANFAPPQRGGQL
                     GWVPLQRVLLTGYLEFPGAQADTVRFLLAHGADPQRKLPFDQGKDVVAFAAQMKSPML
                     ALLQPRTAPALVAPAPALARADTGSLVKTARHDRNEAPPLRAARAGTEASVDSGTATR
                     "
     gene            complement(2754..>3166)
                     /locus_tag="VFF72_13150"
     CDS             complement(2754..>3166)
                     /locus_tag="VFF72_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZV94160.1"
                     /translation="DKRRFTYWLHFAEGSAMPPLLLKLIFDRIGSGQGMPFFAKPVAK
                     GIAARVKGLMVGPNLKRQLDFMEGELGRSEWFAGPEFTAADIQMSFPVEAAAQRAGLD
                     ASRPKLMSFLKRIHARPGYRKALERGGPYSFAND"
BASE COUNT          453 a         1122 c         1120 g          471 t
ORIGIN      
        1 gtcgttgagc gtgtacgtca cggcgacggc gttgccctgg ccgtcgacga tcgagtagtg
       61 cgtcgtgttg ctgccttcgt gcggcgcgat gcccggaccg agcgacttcg aatcggccgc
      121 gcgatccgga tcgatgacgg cccggatctt cgcggcgtag gccttgtcga gcagcctcgc
      181 gatcgggttg gcgacgaagt cgggatcgcc cagttcgctg ttgcgatcga cgtaggcatg
      241 gcgcatcgcc tcgatctgca catgcacgcc cctggccgaa cgaaaccccc acgaggtcag
      301 cggatagcct tcgaggatgt cgaggagctc gcagaggatg acgccccccg agctcggcgg
      361 cggcgccgag acgacgtgat agccgcggta gtcgcactcg acgggcgccc gttcgcgcgt
      421 cgtgtaggca tcgaggtcgg cctgcgtgag caagcccttg ccggcccggc tcgaggcgac
      481 gatggcagcg ccgaccgggc cctcgtagaa gccggccgtg ccggactcgg cgattcgccg
      541 cagcgtttgc gccaggtcgt gctgcaccag ccggtcgccg gctgcgaagg gacggccgtc
      601 ctcgtgcagg aagatggcgc ggctcggcgg atcggcctcg aacgccgccg tcgccgtcgc
      661 gaacatctcg gcatcgccct cgtcgagaac gaatccgcgc tcggcgaagg cgatcgccgg
      721 cgcgatcaat tcggcgcgcg acatcgttcc atagcgggtc cgggcggtct cgagacccga
      781 cacgcttccc ggcacgccgg cggcgaggta gccgcgccgg gtcgatccct tgacgacgtc
      841 gccggcggcg tcgaggtaca tgtcggcgct ggcggccagc ggtgccttct cgcgaaagtc
      901 gagaaaggtc ctgcgcccgt cggcgagctg gatcgtcatg aagccgccac cgccgagatt
      961 gccggcggcc ggatacacga cggcgagcgc gtaaccgacg gcgaccgcag cgtcgacggc
     1021 gttgccgccc ttcttcagga tgtcgacgcc gacttcggtc gccaggtgct gcgccgtcac
     1081 caccatgccg tgctcggcca tgaccggcgc gaccgacgcg gcgccggcac cgccgccggc
     1141 cgcggcggag agggcgacga cgacggcgag gcagcgggaa acgaggcggc aggagatcgt
     1201 cggggacacg tcggggaact cggcgggcga tgcgttgcgg cgccgatgcg gcggcggcct
     1261 tgaccacggc gactgtatcg ccgccgtcgc ggcgcggttg cgacgcggtc ggattccacg
     1321 cctcccgcta cgcgctacac cgtgttaccg actggcccgg ccatcggggc attcttcacg
     1381 aacggccggg agcgccgtcg ataagatcgg cgtccgttcc cactcgctcg cgtgcgcctc
     1441 tcgcctcgcg agtcgggccg acggaggttc gagcagccca tgcgtcgcgt cccgcccctt
     1501 gtcagagccc ttgccttcgc ggcctgcgtc ggcccgatgc tggcggcgtg ctcgccgctc
     1561 agcctcggcc tgaccgcggc aggcgttgcc accgactcga gcatgacgtg ggcgatcgtc
     1621 aagcacgtgc acgcgaagct gacggaggac gatcccacgc cgtgcatcct gctcaacagc
     1681 gtccagcgtg cgctcaacgc ccgctgcgac taccaccccg gcagcattcg cgccgccgat
     1741 ttcgcgtctt cggggttgca ggagtgtccg ctcgccgcgg cgacgcgtga tccgcgtctc
     1801 tggcgcgcct tgcccgagct catcgagaag gggccgggcg tcgacaagtg caacggctcg
     1861 ccgctgctcg cgctggccga gagcgacccg tgtcccgatt tcgcggcggc gtcgccggcg
     1921 gtccggcaat cgctggcgac gctggccgaa agcgatccgc gggcggtgcg ccacgacgtg
     1981 tttcgcatgt tcagctgctc gaacgcccgt gccgtcggcc tcgatcacgt tctcgtgacc
     2041 tggctcgatc acggcgagct ggagccgggc aagctctcgt tcagcccgct gagcgcgctc
     2101 gaccccgaca tgctcgtcgg ccggttcggc cacgagctcg aagtggccgg ccatacgccc
     2161 gaggccgcgc tcgacggcta cgaaggaacg ctgccgagcg gctacgagac ggcgcttcgt
     2221 cgcagcgact gggcggcgct ggagtggtgg ctctatcggt tgccgcagct cgccaatttc
     2281 gcgcctccgc agcggggcgg tcagttggga tgggtgccgc tgcagcgggt gctgctgacc
     2341 ggctacctcg agttccccgg cgcgcaggcc gacaccgtcc gcttcctgct cgcccacggc
     2401 gccgacccgc agcgcaagct gcccttcgac cagggcaagg atgtcgtcgc cttcgcggcg
     2461 cagatgaaga gtccgatgct ggcgctgctg cagccacgca cggcgccggc gctcgtcgcc
     2521 ccggcgccgg ccctggcgcg agccgacacc ggctcgctgg tgaagaccgc gcggcacgac
     2581 cgcaacgagg cgccgccgct gcgcgccgcc cgcgccggca ccgaagccag cgtcgattcc
     2641 ggcaccgcga ctcggtagcg aaagacctcg acggcgccgg ggacgatccg gcgcgcgagc
     2701 cggaacccgg atcgtggcgc gcgtcgtcct gacagcgcag cgcccggcga aactcagtcg
     2761 ttcgcgaagc tgtacgggcc gccgcgctcg agcgccttgc gatagcccgg tcgggcatgg
     2821 atgcgcttca ggaacgacat cagcttcgga cggctcgcgt cgagcccggc acgctgcgcc
     2881 gcggcctcga ccggaaagct catctggata tcggcagcgg tgaactcggg ccccgcgaac
     2941 cactcgcttc ggccgagctc gccttccatg aaatcgagct ggcgcttcag gttgggcccg
     3001 accatcaggc ccttcaccct ggcggcgatg cccttggcga ccggcttggc gaagaacggc
     3061 atgccctggc cggagccgat ccgatcgaag atcagcttga ggagcagcgg cggcatcgcc
     3121 gagccttcgg cgaagtgcag ccagtaggtg aagcggcgct tgtctt
//
LOCUS       DASRWS010000688         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_854714, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000688 DASRWS010000000
VERSION     DASRWS010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854714"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFF72_13155"
     CDS             complement(<1..553)
                     /locus_tag="VFF72_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZV94161.1"
                     /translation="MPVLPRTLEIQSEIAAIRRDIHAHPELAYEENRTSDIVAAKLQE
                     WGLEVTRGLGKTGLVGTLRKGNAVKSIGLRADMDALPMDETNDFAHRSQNAGRMHACG
                     HDGHTAMLLGAAKVLSESRDFEGAVHFIFQPAEEGGGGARSMIEDGLFEKFPCDAVFA
                     IHNKPGIPLGHIVTKPGPLLAAAD"
     gene            complement(629..1123)
                     /locus_tag="VFF72_13160"
     CDS             complement(629..1123)
                     /locus_tag="VFF72_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZV94162.1"
                     /translation="MIELDDIDKRIVAVLQVEGRLSIVDLADRVGLSATPCQRRVKRL
                     EEEGVIGRYAALVSPAAAGINLQALVEVTLDDHSEKTVEAFESAIRARPEVVACYAVT
                     GDMDFLVHVFATDLAQFSDFAMKALLRMPGVKGTRSSFIMQQIKNDLAWAPLPPRVSI
                     QKRA"
     gene            1235..1987
                     /gene="phhA"
                     /locus_tag="VFF72_13165"
     CDS             1235..1987
                     /gene="phhA"
                     /locus_tag="VFF72_13165"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HZV94163.1"
                     /translation="METLAPLDTLRGDYRAMAADWTVPQDLDSYSTEDQAVWRLLAER
                     QTRLAEHHACPEFIQGLRTLGINEAIPDFAEVNRRLETLTGWRVVGVPGLIPDQAFYD
                     HLAHRRFPVTVWIRKRSEIDYLVEPDLFHDFFGHVPLLSNPVFADYMQLYGQRGIEAG
                     PQIDLLARLYWYTVEFGLIRTPQGLKIYGAGILSSAREVRHAIEDDGVERLPFEAARV
                     MRRPYEIDKLQNTYFVLNDFRQLFEAAQTRLA"
     gene            complement(1969..>2505)
                     /locus_tag="VFF72_13170"
     CDS             complement(1969..>2505)
                     /locus_tag="VFF72_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZV94164.1"
                     /translation="LSADIAHKTEAGGVALGLPDGPSAAMAAREIEKRVKAHAPGARL
                     DGFLVQKMERGLAEVILGFRRDPLVGPTVTVGLGGVLAEIYKDAATRIAPVDESEALE
                     MIGEVKGLATIRGYRNLPKGDVAALAKAIATFLALAHSSFGDVSEAEINPLLVKRDGE
                     GVVAVDGLVVLRPQASRV"
BASE COUNT          464 a          838 c          745 g          458 t
ORIGIN      
        1 gatcggcggc agcgagcaac ggtccgggct tcgtgacgat gtggccaagt ggaatgcccg
       61 gcttgttgtg gatcgcaaac accgcgtcgc agggaaattt ctcgaacagc ccgtcctcga
      121 tcatggaacg ggcaccgccg ccgccctcct cggccggctg gaagatgaaa tgcaccgcgc
      181 cttcgaagtc gcggctctca ctcaacacct tggccgcgcc aagcagcatg gcggtatggc
      241 cgtcgtggcc gcaggcatgc atgcgtccgg cattctgcga gcgatgtgca aagtcgttgg
      301 tctcgtccat cggcagcgca tccatgtcag cgcgcaggcc gatcgacttc acggcattgc
      361 ccttgcgaag cgtgccgacc aaccccgtct tgccaaggcc gcgggtaact tcgagccccc
      421 actcctgcag cttggctgcg acgatatcgg aagtcctgtt ctcttcgtac gcgagttcgg
      481 gatgggcgtg gatgtctcgg cggatcgcag caatctcgct ctggatttcc aaagtacgcg
      541 gcaagacggg catcaaacgc ctccagattg aaaaaggggc gcattctaag cggagcgcgg
      601 gttgcagcgc catatgggca gacgattatc acgcgcgttt ttgtatcgaa actctcggcg
      661 gcagcggcgc ccaggccaga tcgttcttga tttgctgcat gatgaaagac gagcgcgtgc
      721 ccttcacacc tggcatgcgc aacagtgcct tcatagcaaa gtcgctgaac tgagcgagat
      781 cggtggcgaa gacatggacc aggaaatcca tgtcgccggt caccgcataa caggcgacca
      841 cttcaggtcg agcgcggatc gccgattcaa aggcttcgac ggtcttctcc gagtgatcgt
      901 cgagcgtgac ctcgaccagc gcctgcaggt tgatgcccgc cgctgccggc gacaccaatg
      961 ccgcatagcg gccgatcacc ccctcctcct ccagccgctt cacgcgacgc tggcagggtg
     1021 tggctgagag gccgacgcga tcagcgaggt cgacgatcga aagccggccc tcgacctgca
     1081 gaacagcgac gatacgttta tcgatgtcat cgagttcgat catggcagtt tccacccgta
     1141 ttggctttgt tcgtagagga tatctccaaa acgcggccga atcgcggctc aatttgccag
     1201 gattcgctcc acccctcgcc tccatattcc acccatggaa accctcgctc ctctggacac
     1261 gctgcgcggt gactaccgtg cgatggcagc cgattggacg gtgccacagg atctcgactc
     1321 ctattcaacc gaggatcagg ccgtatggcg cctgctcgca gaacggcaga cgcggctggc
     1381 cgagcaccac gcttgccccg aattcatcca gggactgcgc acgctcggca tcaacgaggc
     1441 gattcccgat ttcgccgagg tgaaccggag actcgaaacg ctgaccggct ggcgcgtcgt
     1501 cggcgtgccg ggcctcattc ccgaccaggc gttctacgac catctcgccc atcggcgatt
     1561 tccggtcacg gtctggatcc gcaagcgctc ggagatcgac tatctcgtcg agccggatct
     1621 gttccatgac ttcttcggcc atgtgccgct gctcagcaat ccagtgttcg ccgactacat
     1681 gcagctctac ggccagcgcg gcatcgaggc gggtccgcag atcgacctgc tggcgcggct
     1741 ctactggtac acggtcgagt tcggcctgat ccgcacgcca cagggcctca agatctatgg
     1801 cgccggcatt ctctcctctg cgcgcgaggt gcgacacgcg atcgaagacg acggcgtcga
     1861 gcgcctgccc ttcgaggctg cacgcgtgat gcgccggccc tacgagatcg acaagctgca
     1921 gaacacctac ttcgtgctca acgacttcag gcagcttttc gaagccgctc agacccggct
     1981 ggcctgaggt ctcagcacca ccaggccatc gacagcgacg acgccctcgc cgtcgcgttt
     2041 caccagcagc ggattgatct ccgcctccga tacgtcgcca aaactcgaat gcgcgagagc
     2101 caagaaggta gcgatcgcct tggccagcgc cgcgacgtca cctttcggca gattgcggta
     2161 gccacggatg gtcgcgagtc ccttgacctc gccgatcatc tccagcgcct cggactcgtc
     2221 gaccggcgcg atacgcgtcg cggcatcctt gtagatttcc gccagcacgc cgcccagacc
     2281 gaccgtgacc gtgggcccga ccaacggatc ccgccggaag cccaggatca cctcggcgag
     2341 gccgcgttcc atcttctgca ccaggaagcc gtcgagccgg gcgccgggcg catgtgcctt
     2401 cacgcgcttc tcgatctcgc gcgccgccat tgccgccgac ggaccgtcgg gcaggccgag
     2461 cgcgacgccg cctgcttcgg tcttgtgggc gatgtcagcg gaaag
//
LOCUS       DASRWS010000689         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3731702, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000689 DASRWS010000000
VERSION     DASRWS010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731702"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFF72_13175"
     CDS             complement(<1..360)
                     /locus_tag="VFF72_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZV94165.1"
                     /translation="MFAWIGSKFAAILGTYVLGVVSSLMAAITPIALAAMTLWVALYG
                     WAVLRNEVPETLPTFLWKVFKIGLVLAFALQSGLYIASVSDSANSLATGVATTFLPSS
                     ADATTVTSPYALLDTFND"
     gene            complement(977..3148)
                     /locus_tag="VFF72_13180"
     CDS             complement(977..3148)
                     /locus_tag="VFF72_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HZV94166.1"
                     /translation="MPFSSLLGAHDAITRGGDYLRTWRLDGVPFECADDPLVAERHEA
                     LCSLLRNLSGGQWAVWTHRLHRVVRDTMSQPQSGFGRELSRAYYAKLDEQRLMSNELY
                     LTLVYRPHASRVSRALQPTQRSREAIARAQADALGVMAATSALVERVLRGFGPRLLGM
                     RSQGGRDYSELAEFLGYLVNGRWRAIPQTAGPLYRLLPTTRLSFGGDKLELRHGDERR
                     FAALVDLRDYADAVEPGTLNALLYEDSEFIETQSFSILPRREAMRALELQRDQLIASD
                     DAVATQVAEMDVAINELGDGQFCMGEYHYSLVVFGDDVADAGRRAARAMGAIGESSSL
                     QMATVDLVADAAWFAQMPGNWQWRPREAKLSSRAFAALASAHNFASGKRDGNPWGEAL
                     VLLRTPAGQPYYLNLHSSPEGEDSADKKLPGNTLIIGSTGVGKTTLEMFLLTLTRKWS
                     PAPRLVLFDLDRGCEIAIRALGGRYFALEAGKPTGCNPLQGEPTPARLQFWEQLVRTC
                     LSSSTFQLLPADERAIADAVRAVAMMPRRLRRLSTLRQNLPKSGENSLYERLGRWCQG
                     GALGWVFDEADDRLGDLDGAAVIGFDTTEFLDLPEVRTPVMLYLLHVMEGLVNGERLI
                     YVISEFWKALDHDIFSDFAKQKQKTIRKQNGLGIFDTQSPSDVLRHPIGRTMIEQSVT
                     KIFLANPEAVREEYVDGFGLSDAEYQIVRSLGSQGGRRFLV"
     gene            complement(3240..3575)
                     /locus_tag="VFF72_13185"
     CDS             complement(3240..3575)
                     /locus_tag="VFF72_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HZV94167.1"
                     /translation="MVRREAIYKGATRPAMKFGIPLVPLVVLFGAGMLVTIWGGLLVS
                     WWVGAGVVAGIVPALAWMRYVTNKDDQRFRQLFVATTLRLHDRNRSFWMARSYAPTLY
                     RGARDAWHG"
     gene            complement(3581..3871)
                     /locus_tag="VFF72_13190"
     CDS             complement(3581..3871)
                     /locus_tag="VFF72_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HZV94168.1"
                     /translation="MKTISPILVRVQSAVLLLALGSARRASAQGFDKINTTITNVNAI
                     LVTISVAVVTIAIIWAGFKMIFQGARLADVANVLIGGTLIGGAAAFASYIVS"
     gene            complement(3868..4425)
                     /locus_tag="VFF72_13195"
     CDS             complement(3868..4425)
                     /locus_tag="VFF72_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZV94169.1"
                     /translation="MPLSLRRRRPTIQTAADMAATTFLALALSCSPLVDDKTAAALVS
                     VESGFNPNAIGVVGGALERQPRDRREAIATTRALESSGWNFSVGLAQINVRNLQRLGL
                     SVEAAFDPCRNLAAMQALLKDCFDRASGRAHSPQRSLRQALSCYYSGNFVTGFRDGYV
                     SRVVGAARLSGTRPTSLLPTIKESS"
BASE COUNT          785 a         1433 c         1546 g          748 t
ORIGIN      
        1 gtcgttgaag gtgtcaagta gcgcgtaggg cgatgtgacg gtagtggcgt cggcactgga
       61 aggaagaaac gtcgtggcca ccccggtggc gagcgaattc gcggagtccg atacgcttgc
      121 gatgtagagg ccggactgca gcgcgaacgc gaggaccagc ccgatcttga agaccttcca
      181 caggaacgtc ggtaacgtct cgggcacctc gttacgaagg actgcccatc cgtagagggc
      241 gacccatagc gtcatcgcgg cgagcgcgat gggggtgatc gcggccatca acgatgacac
      301 gacaccgagg acgtaggtgc cgaggatggc cgcgaacttc gatccgatcc aggcgaacat
      361 gtgctctgcg tctactggat cgcgtcgtaa ggcaacgact gcagactcgt gcacttgctc
      421 gccagatccg ggtgctggca ctgaagccat cgccctcttg tcttgacgat ccaaatgcgc
      481 ggtagcttcc ggtttccaaa cgttgaggcg cgacacgttc cggggcgcgc atagagctca
      541 gtgcattctt caaggtcacc cggcgtctcc accatcttcc agcctccgct tccgcagccg
      601 acctgtttgg ccgggcaagg cttcgaaacg aagctcgaag gaactggcgg cgtctcgacg
      661 cgcttcctcc catcgggcat gacggtgacc gcttcgttgc ccctgacgaa ctgaccgaag
      721 gacgtggtca cggcaggaga aagagacaag gcgacgaagg cgagactcga ggctttcatg
      781 cgaccctccg acgcgcccga atccttcgcg cctgcacctc cgacaagagg atgggaagcc
      841 actccgcagg gtcatcgccg acgcgcgcac ggatgtcgtc gaggagggcg acgttgtcgg
      901 tcgtcgccga cagcaccgtc acgaaatcgt ccagcccctc gaggtcgagt tcgcaaatgg
      961 cgctgctgtg gacttgctag accagaaagc gccggcctcc ctgcgacccg aggctgcgca
     1021 cgatctggta ttccgcatcg ctcaggccga aaccgtccac gtattcctcg cgcaccgctt
     1081 cggggttggc gaggaagatc ttggtgacgc tctgctcgat catggtccgg ccaatgggat
     1141 ggcggagcac gtccgaggga ctctgcgtgt cgaagatgcc caggccgttc tgcttcctga
     1201 tcgtcttttg cttttgcttg gcgaagtcgc tgaagatgtc gtggtcgagc gccttccaga
     1261 actcggagat gacgtagatc aggcgctcgc cattgacgag cccctccatc acgtgcagga
     1321 ggtagagcat caccggcgtg cgcacttcgg gcaggtcgag gaactcggtc gtgtcgaagc
     1381 cgatgactgc agcaccatcc aggtcgccca gtcggtcatc agcctcatcg aagacccagc
     1441 caagcgcacc accctggcac cagcgaccga gccgctcgta gaggctgttc tcgccgctct
     1501 tgggcaggtt ctggcgcagg gtcgagaggc ggcgcaggcg gcgcggcatc atcgccaccg
     1561 ccctgacggc gtcggcgatc gctcgctcgt ctgccggcag gagttggaag gtcgagctcg
     1621 aaaggcacgt gcggacaagc tgctcccaga actggaggcg tgccggcgtc ggctcgccct
     1681 gcagcgggtt gcagccggtg ggcttgcccg cttcgagggc gaagtagcga ccgccgagcg
     1741 cccggatggc gatctcgcag ccacggtcga gatcgaagag gacgagccgt ggcgccggac
     1801 tccacttgcg ggtcagtgtc agcaggaaca tctccagcgt cgtcttgccg acgccggtcg
     1861 agccgatgat cagcgtattg cccggcagct tcttgtcggc cgagtcctcg ccttcggggc
     1921 tgctgtgcag gttgaggtag tagggctggc cggccggtgt gcgcagcagg acgagggctt
     1981 ctccccacgg gttgccgtcg cgcttgccgc tggcgaaatt gtgggcgctg gcgagtgcgg
     2041 caaaggcgcg cgaggagagc ttggcctcgc gcggccgcca ttgccagttg cccggcatct
     2101 gcgcgaacca ggcggcatcg gcgacgaggt cgaccgtagc catctgcaag ctcgacgact
     2161 cgccgatggc acccatcgcc cgggccgcgc ggcgcccggc atcggccaca tcgtcgccga
     2221 agacgaccag gctgtagtgg tactcgccca tgcagaactg gccatcgccc agctcattga
     2281 tcgcgacgtc catctcggcg acctgggtgg cgacggcgtc gtcgctggcg atcaactggt
     2341 cgcgctgcag ctcgagggcg cgcattgctt cgcgccgcgg caggatcgaa aagctctgcg
     2401 tttcgatgaa ctcgctgtct tcgtagagga gcgcgttcag ggttccgggc tcgacggcat
     2461 cggcgtagtc gcgcaggtcg acgagcgcgg cgaagcgacg ctcgtccccg tgacgcagct
     2521 ccagcttgtc gcccccaaag gaaagtcgcg tcgtcggcag caggcgatag agcgggccgg
     2581 cggtctgtgg gatggcgcgc cagcgcccgt tgacgagata gcccaggaac tccgccagtt
     2641 cggaataatc ccgcccgccc tgggaacgca tccccagcag tcggggaccg aagccgcgca
     2701 gcacgcgctc gacgagggcg ctcgtcgctg ccatcacgcc aagagcgtcg gcctgggccc
     2761 tggcgatggc ttcgcgcgag cgctgggtgg gctgcagcgc ccggctgacc cgcgatgcgt
     2821 gcggtcgata gacgagcgtc agatagagct cgttgctcat cagccgctgc tcgtccagct
     2881 tcgcgtagta ggcacgggag agctcgcggc cgaatccgga ctgtggctgg ctcatcgtgt
     2941 cgcgtaccac gcggtgcagc cgatgcgtcc agaccgccca ctggccaccg gagaggttgc
     3001 gcagcaggct gcacagggcc tcgtgccgct cggcgacgag cgggtcgtcg gcgcattcga
     3061 atggcacgcc gtcgagacgc cacgtgcgca gatagtcgcc gccgcgcgtg atggcgtcgt
     3121 gcgcaccgag gagggatgag aagggcacga acctgacaag tgggttctcg cgcgcggcct
     3181 gagaccagta gcgcggtgcc ggcgccggtc gcccaccgct cgccgctgcg gactgacctc
     3241 tagccatgcc aggcatcgcg ggctcctcga taaagcgtcg gggcgtagct gcgcgccatc
     3301 cagaagctgc gattgcgatc gtgcaggcgt agcgtggtcg cgacaaagag ctggcggaat
     3361 cgctggtcgt ccttgtttgt gacgtagcgc atccaggcca gggcgggaac gattccggcg
     3421 acgactccgg cgccgaccca ccagctgacg agaagcccac cccagatcgt caccagcatg
     3481 ccggcaccga acaggaccac caggggcacg agcgggatgc cgaacttcat cgccggcctc
     3541 gtcgcgcctt tgtagatggc ttcacgtcgg accatgacgc tcagctgacg atgtagctgg
     3601 cgaaggcggc ggcgccgccg atgagggtgc caccaatcag gacgttggcg acgtcggcca
     3661 gacgggcgcc ctggaagatc atcttgaagc cggcccagat gatggcgatc gtcaccaccg
     3721 ccacggagat ggtgaccagg atcgcgttga cgttggtgat ggtcgtgttg atcttgtcga
     3781 acccttgggc cgacgcgcga cgcgccgagc cgagggcgag cagcaggacc gcggactgaa
     3841 cgcgcacgag gatcggagaa atcgtcttca tgaggactcc ttgatggtgg ggagcaggga
     3901 cgtgggcctg gtgcccgaaa gacgtgcggc gccaacgaca cgactgacgt agccgtctct
     3961 gaagccggtg acgaaattgc cgctgtagta gcaggagagg gcttggcgca gcgaccgctg
     4021 cggcgagtgg gcgcgcccgc tggcgcggtc gaaacagtcc tttagcagcg cctgcatggc
     4081 cgcaaggttt ctgcacggat cgaaggcagc ctcgacgctc agaccgagcc gctgcaggtt
     4141 gcggacgttg atctgcgcca ggccgacgct gaaattccag ccgctcgact ccagcgcgcg
     4201 tgtcgtggca atggcttccc tgcggtcgcg cggttgtcgc tccagcgctc caccgacgac
     4261 gccgatcgcg tttggattga agccgctctc gacgctcacg agcgctgctg ccgtcttgtc
     4321 gtcgacgagc ggtgagcaac tcaacgccag ggccaggaaa gtcgtcgccg ccatgtcagc
     4381 cgccgtttga atcgttggcc ggcggcgccg gaggctgagc ggcaagccac gccgcgtagt
     4441 cgtcgtcaaa gcgcgtgtcc caggtgccga gttgcgcctt ggcggccggc gtgaactcgg
     4501 tgacgctgcc gc
//
LOCUS       DASRWS010000690         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3146001, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000690 DASRWS010000000
VERSION     DASRWS010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146001"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..385
                     /locus_tag="VFF72_13200"
     CDS             68..385
                     /locus_tag="VFF72_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94170.1"
                     /translation="MLLEGIVFAGGQRALLEELEDGALPGVREGVRRVDLDERWHIGF
                     GLRCLIDAKPPPDLITALLASAADAAAAWGDAVPLETSRHVLRMCARRLAIAGLIKFQ
                     AAA"
     gene            493..735
                     /locus_tag="VFF72_13205"
     CDS             493..735
                     /locus_tag="VFF72_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94171.1"
                     /translation="MQTLAELGPAKLLDDEVDRLREAADTLLFTENMADDAAVVATRE
                     ARSVIDNLVDSGRWSEERANRLADDLGACGPLAPVA"
     gene            complement(747..2084)
                     /locus_tag="VFF72_13210"
     CDS             complement(747..2084)
                     /locus_tag="VFF72_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZV94172.1"
                     /translation="MEPRLLGPGRLIGGVLALAGAAPLFIVSTTTRTPETAAATSEMT
                     AICLVLAVGFLGPVVAHAAARLLTPALASLSPVGGFLASANLATATRRFSSASTPIVL
                     TVAMSCTLLFSTTTIDHAASAQRDAAITSTLAITSAGPGLPPAALADVRATPGVRSAA
                     ALTSTTLGPSLGASDDTIPAQVLDGDRGDAGLDLGVTAGSLARLHGKAIALGRRRAAS
                     AHAHVGQRVPVTLGDGTHTEATVVAIYDRELAFGEAFLAPELAAGHRTTSLLGTILVQ
                     TDHPAAVARRLGALGSRYPGLAVSDRASVATAADADGEMNRWLGPIFVAMIFAFTSIA
                     VINTLAMIALRRGRELALLRLVGATARQVRSMARWEAALIIAMGLGVGLAIAATALLP
                     LSHALTGGLRPHVPARPLVAILGISALLALVALAVPTRRALRARPIEAIGVRE"
BASE COUNT          328 a          745 c          791 g          281 t
ORIGIN      
        1 tccggcgatt gcggctgagc tcgcggaagg tcgtacgtcg ctcgggaacg gcgtgagcct
       61 gtaccacatg ctgctcgagg gcatcgtgtt cgccggcggg caacgcgcgc tgctcgagga
      121 actcgaagac ggcgccctgc ccggcgtgcg cgagggcgtg cggcgagtcg acctcgacga
      181 acgctggcac atcggcttcg gcttacgctg cctgattgac gcgaaacctc ccccagacct
      241 gatcaccgcg ctgctcgcgt cggccgccga cgccgccgcc gcgtggggtg acgctgtccc
      301 gctcgaaacg agcagacacg tgttacggat gtgcgcacgg cgcctggcga tcgccgggct
      361 catcaagttt caagcagcgg cgtagcaggg cgacgacctg cgttgcgccg gatgcgcctc
      421 gatttcgagg aacgcacgtt cgctagactc gggtccatga cctcagaccg ttcacaggca
      481 tacgggcggg tgatgcagac gctcgccgaa ctcggcccgg caaagctgct cgacgatgag
      541 gtggatcgcc tcagagaagc agccgacacc ttgcttttta ctgagaacat ggccgacgac
      601 gccgcggtag tcgcgacccg cgaggcgcgc agcgtcatcg acaacctcgt cgactcaggc
      661 cgctggagcg aggagcgcgc caatcggctg gccgacgacc tgggcgcgtg cggcccgctg
      721 gcaccggtcg cctgaggtcg cctgacttac tcgcggacgc cgatcgcctc gatcggccgc
      781 gcccggagcg cacgccgcgt cggcaccgcc agcgcgacca gcgccagcag cgcggagatc
      841 cccaggatcg ccacgagcgg gcgggccggc acgtgcgggc gcaggccgcc ggtcaatgcg
      901 tggctcagcg gcagcagcgc ggtggcggcg atcgccagtc ccacgccgag gcccatggcg
      961 atgatcagcg ccgcctccca gcgcgccatc gagcggacct ggcgggcggt ggcgccgacg
     1021 agccgcagca gcgccaactc gcgtccgcgc cgcagcgcga tcatcgccaa cgtgttgatc
     1081 accgcgattg acgtgaaggc gaagatcatc gccacgaaga tcgggcccag ccaccggttc
     1141 atttcaccgt cggcgtcggc tgcggtcgcg accgacgccc ggtcgctgac tgcaaggccg
     1201 gggtagcgcg aacccagggc gcccagccgc cgcgcaactg cggccgggtg atcggtctgc
     1261 acgaggatcg tgcccagcag cgaagtggtt cgatggccgg ctgccagctc gggtgccagg
     1321 aaggcctcac cgaaggcgag ttcgcggtcg tagatcgcca ccacagtggc ctctgtgtgc
     1381 gtgccgtcgc cgagtgtgac gggtacacgc tggcccacgt gggcgtgcgc gctggcggcc
     1441 cggcggcgac cgagcgcgat cgctttgccg tgcagcctcg ccagcgagcc ggcggtcacg
     1501 ccgaggtcga ggcccgcgtc accgcgatcg ccgtcgagta cctgcgcggg gatcgtgtcg
     1561 tcggaggcac cgaggctcgg cccgagcgtc gtcgacgtca aagcggccgc ggagcggacg
     1621 ccgggcgtgg cgcggacgtc ggcgagcgct gccggcggca ggcccggacc ggcgctggtg
     1681 atcgcgagcg tgctggtgat cgccgcgtcg cgttgggcgc ttgcggcatg gtcgatcgtg
     1741 gtcgtgctga acagcagcgt gcagctcatc gcgaccgtga ggacgatcgg cgtgctggcc
     1801 gatgagaagc gtcgcgtggc ggtggccaga ttcgccgagg cgaggaagcc gccgacgggc
     1861 gacagcgaag ccagcgccgg cgtcagcagc cgcgcggcgg catgcgccac gaccggcccg
     1921 aggaagccga ccgccagcac caggcagatc gcggtcatct ctgacgtcgc ggccgccgtc
     1981 tcgggcgtcc tggtggtcgt cgagacgatg aacagcgggg ccgcacctgc cagcgcgagc
     2041 acgccgccga tcagacgtcc cggcccaagc agccgcggct ccacggcggc gtctccgagc
     2101 gcgagcgtcg gtgcgacgcg agcggcgcgc cgcgaagctc ccagg
//
LOCUS       DASRWS010000691         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2572243, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000691 DASRWS010000000
VERSION     DASRWS010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572243"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFF72_13215"
     CDS             complement(<1..960)
                     /locus_tag="VFF72_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV94173.1"
                     /translation="MNIPSLLSPLRLAVAAVAVGAMSSAFAADIKLGAAEALSGPAGQ
                     YGQSIRNGLQLAVDEINAAGGVNGDKLSLQVEDEQGKKEQAIDVFKKLIFQDRVLMLF
                     GPTLSNSAQAADPIAQAAHIVAFGTSNTAAGITSIGDYIFRNSVTEADVLPETLRVAV
                     KHAHIKKVAILYGNDDVFTKSGYDAFKKALADLNIPVTATETFAKGDVDFKAQLTKIK
                     ATGPDALVLSALIAEGAPIMVQARQIGLNVPVIGGNGMNSVKIFDLAKGASDGLWVGS
                     PWSIENRTPENNSFIIAYTKKYSAPPDQFAAQAYDAMHIATVAL"
     gene            complement(1062..2459)
                     /gene="pmbA"
                     /locus_tag="VFF72_13220"
     CDS             complement(1062..2459)
                     /gene="pmbA"
                     /locus_tag="VFF72_13220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZV94174.1"
                     /translation="MPETTAASLSPTEFAYSHERFRELAEGALAAARGLGASDAVAEV
                     SEGVGLSVSVRKGETENVERNRDKSLSVTVYAGQRRGNASTSDFSPGALQQTVRAAWD
                     IARFTAADPAAGLPDADDLALGRAAERDLDLFHPWPIDADAAIRIAGECEAAALSVDR
                     RITNSEGAGVSAQQSHFYAANTRGFGAGYASSRHSLSVAPIASLPGRGGDDMQRDAWY
                     TSMRSPGEMAAPAAVGRYAAERALSRLRARKIKTCEVPVLFESALASGLLGAYVQATS
                     GGALYRKSSFLEGSLGTQVLADHVDVREDPHQRRGKGSAPFDDEGVVTKARDIVVAGI
                     TQSYFLSTYSARKLGMKTTGHAGGSHNMVLTSRLTRPGDDLAAMLKKLHRGLFVIELM
                     GQGVNYVTGDYSRGAAGFWVENGEIAFPVHEITIAGNLRSMLRSIVAVGADAYTMGSK
                     TTGSVLIERMKIAGA"
     gene            2623..>2900
                     /gene="yjgA"
                     /locus_tag="VFF72_13225"
     CDS             2623..>2900
                     /gene="yjgA"
                     /locus_tag="VFF72_13225"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZV94175.1"
                     /translation="MAMPRPSKTERKTASHELQSLGAALLEMNDERIASLGLDEPLLD
                     ALRAHRKMRSHEARRRQMQLIGKLMRSADVEPVRAAVAEAQLGRASDAL"
BASE COUNT          458 a          951 c         1019 g          472 t
ORIGIN      
        1 gagcgccacc gtggcgatgt gcatcgcgtc gtaggcctgg gcggcgaact ggtcgggcgg
       61 cgcgctgtac ttcttggtgt acgcgatgat gaagctgttg ttctcgggcg tgcggttctc
      121 gatcgaccac gggctgccca cccacaggcc gtccgacgcg cccttggcga gatcgaagat
      181 cttcacggag ttcatgccgt tgcccccgat caccggcacg ttcaggccga tctgccgtgc
      241 ctgcaccatg atgggcgcgc cctctgcgat gagggccgac agcaccagcg cgtccgggcc
      301 ggtggccttg atcttcgtga gctgcgcctt gaagtcgacg tcgcccttgg cgaaggtctc
      361 ggtcgcggtg accgggatgt tcagatccgc gagtgccttc ttgaaggcgt cgtagccgct
      421 cttcgtgaag acgtcgtcgt tgccgtagag gatggcgacc ttcttgatgt gcgcgtgctt
      481 gacggccacg cgaagcgtct cgggcaggac gtcggcctcg gtcaccgaat tgcgaaagat
      541 gtagtcgccg atcgacgtga tgccggccgc ggtgttcgag gttccgaagg cgacgatgtg
      601 cgcggcctgg gcgatcgggt cggcggcctg cgccgagttc gacagcgtcg gcccgaagag
      661 catgagcacg cgatcctgga agatgagttt cttgaaaacg tcgatcgcct gctctttctt
      721 gccttgttcg tcttcgacct ggagcgaaag cttgtcgcca ttgacgccgc cggcggcatt
      781 gatctcgtcg accgcgagct gcaggccgtt gcggatcgac tggccgtact ggccggccgg
      841 accggagagt gcctcggcgg cgccgagctt gatgtcggcg gcgaaggccg acgacatcgc
      901 gccgaccgcg acagcggcga cggctaggcg aagtggcgag agaagcgagg ggatgttcat
      961 gaacgcgtga ctccgatgac gggtcgccca acgggcggaa agcagcgatt atcgttcgcc
     1021 gccccggggc ggcgtctcgg gaaactaccc gcgcagcgaa gtcaggcgcc ggcgatcttc
     1081 atccgctcga tcagcaccga gccggtcgtc ttgctgccca tggtgtacgc atcggcgccg
     1141 acggcgacga tcgaacgcag catcgaacgc aggttgccgg cgatcgtgat ctcgtggacc
     1201 ggaaacgcga tctcgccgtt ctcgacccag aaaccggctg cgccgcgcga gtagtcgccg
     1261 gtgacgtagt tgacgccctg gcccatcagc tcgatcacga aaagcccccg atgcagcttc
     1321 ttcagcatgg cagcgagatc gtcgcccggc cgggtgagcc gcgaggtgag caccatgttg
     1381 tgcgatccgc cggcgtggcc ggtggtcttc atgccgagct tcctcgccga atacgtcgac
     1441 aggaaatagc tctgcgtgat gccggcgacg acgatgtcgc gcgccttcgt cacgacgccc
     1501 tcgtcgtcga agggcgcgct acccttgccg cggcgctgat gcgggtcttc gcggacgtcg
     1561 acgtgatcgg caaggacctg cgtgccgagg ctgccttcca ggaacgacga cttgcgatag
     1621 agggcgccgc cactcgtcgc ctgcacgtaa gcgccgagaa gccccgaggc gagggccgat
     1681 tcgaaaagca ccggcacttc gcacgtcttg atcttgcgtg cacgcagccg cgaaagagcg
     1741 cgctcggcgg catatcggcc gaccgccgcg ggggccgcca tttcgccggg cgagcgcatc
     1801 gacgtgtacc acgcgtcgcg ttgcatgtcg tcgccgccgc gcccgggcag cgacgcgatc
     1861 ggcgccaccg acagggagtg ccgcgagctc gcgtagccgg cgccgaagcc gcgcgtgttc
     1921 gcggcataga agtgcgactg ctgcgccgag acgccggcgc cttcggaatt ggtgatgcgg
     1981 cggtcgaccg agagcgccgc ggcttcgcat tcgccggcga tgcggatcgc cgcgtcggca
     2041 tcgatcggcc agggatggaa aaggtcgaga tcgcgttcgg cagcgcggcc gagggcgagg
     2101 tcgtccgcat cgggcaagcc ggcggccgga tcggcggcgg tgaagcgggc gatgtcccag
     2161 gccgcgcgga cggtctgctg cagggcgccg ggagaaaagt cggacgtgct cgcgttgccg
     2221 cgacgctgac ccgcgtagac cgtgacgctc aacgacttgt cgcggttgcg ctcgacgttt
     2281 tccgtctcgc ccttgcgcac cgagaccgac aggccgacgc cctcggaaac ctcggccacc
     2341 gcgtcgctgg cgcccaaccc gcgcgccgcc gccagcgcgc cctcggcgag ctcacgaaat
     2401 cgttcgtgcg aataggcgaa ctcggtgggc gaaagcgaag cggcagtcgt ttcaggcatg
     2461 gtcgggggct gtggcgccgg cggcggacgg cgccgggcag agggtggcag atatcatagc
     2521 gacgcctctt ttgccttctt tgtgcgagtg ctgcagccct tgcgatcccg ccccccgcct
     2581 cgagagtcga ctccgtcgtc cgccgacccg accggcgcgg tcatggccat gccgcggccg
     2641 agcaagaccg aacgcaagac cgcttcgcac gagctgcagt cgctcggcgc cgctcttctc
     2701 gagatgaacg acgaacgaat cgcctcgctc ggcctcgacg agccgctgct cgacgccctg
     2761 cgcgcacacc ggaagatgcg cagccacgag gccagacgcc ggcagatgca gctgatcggc
     2821 aagctgatgc gctcggccga tgtcgagccg gtgcgggcag cggtcgccga agcgcagctc
     2881 ggccgggcca gcgacgcgct
//
LOCUS       DASRWS010000692         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_4299426, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000692 DASRWS010000000
VERSION     DASRWS010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4299426"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..702
                     /locus_tag="VFF72_13230"
     CDS             151..702
                     /locus_tag="VFF72_13230"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HZV94176.1"
                     /translation="MDTKHRATPAALRSRGLSLIELAITLAVVAIVAASAAPSLASLI
                     DARRLDAAANELAADVQWVRTEAVARNRPVRLSFHSSADASCWIVHTGAAAQCRCGSD
                     ATPVCAGDAIGIKSVLLVGAERVAVAANVASIVFDPLHGTATPTGTLRLIDVHGRAVH
                     HVVNIMGRVRSCTPSSLAGWRAC"
     gene            728..1231
                     /locus_tag="VFF72_13235"
     CDS             728..1231
                     /locus_tag="VFF72_13235"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HZV94177.1"
                     /translation="MTSLVPRPTFEPAPPFAAAAASRARPRQRGFTLVETLVAVGIAG
                     VLSSIAYPSLSGHLSRARRADALVALLQAQLAQERFRADHASYGNLADIGVRSTSTSG
                     FYSLTVDSADTAGCRLVATATGAQARDTTCRTLRLEVTGSAIAYTSGADAATANAADI
                     NRRCWNR"
     gene            1231..1914
                     /locus_tag="VFF72_13240"
     CDS             1231..1914
                     /locus_tag="VFF72_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZV94178.1"
                     /translation="MRARRCRGLSLVELLVGTAIGLLVVAAASTQVVHHVRDSRALAI
                     ETRLMQELRTASDIVARDLRRAGYWAGAGSGVASQDGSPGVANPYAAASPEIAASDTV
                     RLRYSRDAVENDSVDSNEQFGFRLRGGAIEMQLGDANWQSLTDARTLQVTEFSVEPRI
                     EESSLAEFCAGPCAAGDTACPPRQQTRRFAVRISGRSAFDPAIRRSVRAEARVRNGTV
                     VGSCAGSGS"
     gene            1911..>2495
                     /locus_tag="VFF72_13245"
     CDS             1911..>2495
                     /locus_tag="VFF72_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HZV94179.1"
                     /translation="MKSALRDRRQRGAAALLVTALLFLTLLVVVAATQRQVLVEVGSS
                     LGQQRSTQAFEAAEAGLEWAVARLNDDTRLGDDCLPSSAVDAASFRDRHLPLDLSLGR
                     RRAATWDDAGTPRLLQAVCVHGDAGWTCSCPASGPPSPAAGDVPAPAFAVVFGEGPRP
                     DVFTAVASGCLQGRTPCADTAASKHEAAARLEVAV"
BASE COUNT          356 a          930 c          819 g          390 t
ORIGIN      
        1 gacgagttcc gccagctgat ccgcgacatc tgatcctgcc tgtggcgctt ccggctgcca
       61 ccccccgaac gaacgcaccg ccgggactcc cccgcgtctc cccagccgac gggatcgcga
      121 aatagcgcac gaatttctac agtgcttcgc atggacacga agcaccgcgc aacccctgcc
      181 gcacttcgca gccgcggcct ttcgctgatc gagctggcga tcaccctggc cgtcgtcgcg
      241 atcgtcgcgg cgagcgcggc accgagcctc gccagcttga tcgacgcccg ccggctcgac
      301 gcggccgcga atgaactggc cgccgatgtg caatgggtga ggaccgaggc ggttgcgcgc
      361 aaccggccgg tgcggctcag ctttcacagc agcgccgacg ccagctgctg gatcgtgcac
      421 accggcgcgg cggcccagtg ccgctgcggc agcgacgcga cgccagtctg cgctggcgac
      481 gcgatcggca tcaagtcggt ccttctggtc ggcgccgaac gcgtcgcggt cgccgccaac
      541 gtcgcttcca tcgtcttcga tccgctgcac ggcaccgcga cgccgaccgg cacgctgcgc
      601 ctgatcgacg tgcacggtcg cgccgtccac cacgtcgtca acatcatggg ccgcgttcgc
      661 tcgtgcacgc cgagcagcct cgccggctgg cgcgcctgct gaacgcgatt tgcgaggagc
      721 accttcgatg acttcactcg tcccccgacc gacgttcgag ccagcgccgc cgtttgcagc
      781 ggcagctgcc tcgcgggcgc ggccgagaca acgcggattc acgctcgtcg aaacactcgt
      841 cgcggtcggc atcgcgggcg tcctgtcgag catcgcctat ccgtcgctga gcggccatct
      901 gtctcgagcg cgccgtgccg atgccctggt cgcgctcctc caggcccagc tcgcccagga
      961 gcgatttcga gccgatcacg caagctacgg caatctcgcc gatatcggcg tgcgctcgac
     1021 gtcgacctcc ggcttctatt ccctcaccgt cgactccgcc gacaccgccg gctgtcgcct
     1081 cgtcgcgacg gccaccggag cgcaggcacg cgatacgacg tgccgcacgc tgcgtctcga
     1141 agtcaccggt tcggccatcg cctacacgtc gggcgccgat gccgcaacgg ccaatgccgc
     1201 tgacatcaac cgccgctgct ggaaccggtg atgcgtgccc gtcgttgccg tgggctgtcg
     1261 ctggtcgagc ttctcgtcgg cacggcgatc ggcctgctcg tggtcgccgc cgcctcgacc
     1321 caggtcgtcc accacgtccg ggatagccgc gcactggcga tcgagacgcg cctgatgcag
     1381 gaactgcgca cggcgagcga catcgtcgcc cgcgatctgc gccgcgccgg ctattgggcc
     1441 ggcgcaggct ccggcgtcgc gtcgcaagac ggcagccctg gcgttgccaa tccgtacgct
     1501 gccgccagtc ccgagatcgc cgcatcggat acggtgcgcc tgcgctactc gcgcgacgcc
     1561 gtcgagaacg acagcgtcga cagcaacgag cagttcggct ttcgcctgcg cggcggcgcc
     1621 atcgagatgc agctgggcga cgccaactgg cagtcgctca ccgatgccag aacgttgcag
     1681 gtcaccgaat tcagcgtcga accccggatc gaggaatcga gcttggcgga attctgcgcc
     1741 ggcccctgcg ccgccggcga cacggcctgt ccaccgcggc agcaaacgcg ccgcttcgcg
     1801 gtcaggatct cgggccgctc ggcattcgat ccggccattc gccgcagcgt ccgcgccgag
     1861 gcccgggtgc gcaacggcac cgtcgtcggc agctgtgcgg ggagtggatc atgaagtctg
     1921 cattgcgcga ccgtcgccaa cgcggagcgg ctgccttgct cgtcaccgcc cttctctttc
     1981 tgacgctgct cgtcgtcgtc gccgcgacgc agcggcaggt tctcgtcgag gtcggatcgt
     2041 cgctcggcca gcaacgctcg acgcaggcgt tcgaggccgc cgaggcaggg ctcgaatggg
     2101 ctgtcgccag gctcaacgac gacacgcgcc tgggcgacga ttgcctgccg agcagcgcag
     2161 tcgacgcggc gtcgtttcgc gatcgccatc tcccgctcga cctgtcgctg ggaaggcgac
     2221 gggcggccac gtgggacgac gccggaacac ctcgcctctt gcaggcggtc tgcgtgcatg
     2281 gcgacgccgg ctggacatgc agttgtccgg cgagcggccc gccatcgcca gcggccggtg
     2341 acgtgcccgc gcccgccttc gccgtcgtct tcggcgaggg gccgcgtccg gatgtcttca
     2401 cggcggtcgc gagcggttgc ctgcaaggcc gcacgccgtg tgccgacact gcggcctcca
     2461 agcatgaagc ggcggcccgg ctcgaagtcg ccgtc
//
LOCUS       DASRWS010000693         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_287410, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000693 DASRWS010000000
VERSION     DASRWS010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287410"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1125)
                     /locus_tag="VFF72_13250"
     CDS             complement(145..1125)
                     /locus_tag="VFF72_13250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV94180.1"
                     /translation="MIYTHLGRTGIEVSRICLGCMSYGDPERGNHAWTLGEAASRPFL
                     RRALDLGINFFDTANVYSAGTSEEIVGRALLAYARRGEVVIATKVHGRMHKGPNGAGL
                     SRKAIFEEIDASLKRLGTDHVDLYQIHRFDDAVPIEETLSALNDVVRAGKALHIGASS
                     MHAWQFARALHLSERNGWARFATMQNYLNLLYREEEREMLPLCRFEGIGVIPWSPLAR
                     GRLTRPWDETTTRSESDGFGRSLVARSADADRKVVEALGAVATRLDRPRAQVALAWVL
                     AKPEVTSPIVGATKIEHLDDAIAALSLALSPGDVAELEAPYVPHAVVGHA"
     gene            complement(1150..2007)
                     /locus_tag="VFF72_13255"
     CDS             complement(1150..2007)
                     /locus_tag="VFF72_13255"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZV94181.1"
                     /translation="MAFTSDPAIDKAYSPSRSLPPGVFDAHMALYRERSRAVYDAFAT
                     ERDLCYDAASGQCLDIVPAPGPGLKPAFVFIHGGYWRMLSKNESVYMAPPLAARGISL
                     VAPDYALAPGATLEEIVRQVRASIAWLHRHGAGHGIDPERIFVGGHSAGGHLTAMLVA
                     SGWHAAMDLPEDVIKGAMPVSGLFDLEPITHSFVNEWMGLDDARAAALSPIHRLPAQP
                     CRMVVAWGGDETSGFHDQSLAWLGAWRGAGFAATELMVPGRHHFDIVLDACDADSAMS
                     RACAAMILG"
     gene            complement(2011..>2168)
                     /locus_tag="VFF72_13260"
     CDS             complement(2011..>2168)
                     /locus_tag="VFF72_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZV94182.1"
                     /translation="GQAILVIDKYVQRLIRLADRHVILEKGRVAWSGSSAELDADASI
                     WQRYLGV"
BASE COUNT          322 a          743 c          768 g          335 t
ORIGIN      
        1 ggcgaggcgc gcaaggaccg cgagcacgac aaggaccaga cgccccggta agcgtggccg
       61 tccgacgcag cggcggcatc cgtcggcgct gccagcgggg gccgcgtcgc gggggactgg
      121 cggctttggc gggcggcagc cggctcaggc gtggccgacg acggcgtgcg gcacgtaggg
      181 cgcttccagc tcggccacgt cgcctggcga cagggcgagc gacagcgccg cgatcgcgtc
      241 gtcgagatgc tcgatcttgg tcgcgccgac gatcggcgag gtgacctccg gtttggccag
      301 cacccaggca agcgcgacct gcgcgcgcgg ccggtcgagc cgtgtcgcga cggcgccgag
      361 cgcctcgacg accttgcgat cggcgtcggc gctgcgggcg acgagacttc ggccgaagcc
      421 gtcggattcg ctgcgcgtcg tcgtctcgtc ccacggtcgc gtcagccggc cacgggcgag
      481 cgggctccac ggaatcacgc cgatgccttc gaacctgcac agcggcagca tctcgcgctc
      541 ctcctcgcgg tagagcagat tgaggtagtt ctgcatcgtc gcgaagcgcg cccagccgtt
      601 ccgctcggac agatgcagcg ccctcgcgaa ctgccaggcg tgcatcgacg acgcgccgat
      661 gtgcaatgcc ttgccggcgc gcacgacgtc gttcagcgcc gagagcgttt cctcgatcgg
      721 caccgcatcg tcgaagcggt ggatctggta gaggtcgacg tggtcggtgc cgagtcgctt
      781 caggctggcg tcgatctcct cgaagatcgc ctttctcgac aggccggcgc cgttcgggcc
      841 cttgtgcatg cgaccgtgca ccttggtcgc gatcaccacc tcgccgcgcc gtgcgtaggc
      901 gagcagggcg cggccgacga tctcctcgct ggtgcccgct gaatagacgt tggcggtgtc
      961 gaagaagttg atgcccagat ccagtgccct tctgaggaag ggccggctcg ccgcctcgcc
     1021 gagcgtccag gcgtgattgc cgcgctccgg gtcgccatag ctcatgcagc cgaggcagat
     1081 gcgcgagacc tcgatgccgg tgcggccgag gtgggtgtag atcattgggt gcatgggcgc
     1141 agtgtcgtct caaccgagga tcatcgccgc gcaggcgcgg ctcatcgccg aatcggcatc
     1201 gcacgcgtcg agcacgatgt cgaagtgatg ccgcccgggc accatcagct ccgtcgctgc
     1261 gaagccggcg ccgcgccacg cgccgagcca ggccagcgac tggtcgtgaa agccgctcgt
     1321 ctcgtcgccg ccccaggcga cgaccatccg gcaaggctgt gccggcagcc ggtggatcgg
     1381 gctgagcgcc gcggcgcgcg cgtcgtcgag ccccatccat tcgttgacga acgaatgcgt
     1441 gatcggctcg aggtcgaaca ggccgctcac cggcatcgcg cccttgatga cgtcttcggg
     1501 caggtccatc gcggcatgcc agccgctcgc cacgagcatg gccgtcaggt gtccgccggc
     1561 cgaatggccg ccgacgaaga tgcgctccgg atcgatgccg tgcccggcgc cgtgccgatg
     1621 cagccaggcg atcgaggccc gcacctggcg aacgatttcc tcgagcgtcg cgcctggcgc
     1681 gagcgcgtag tcgggcgcga ccaggctgat gccgcgggcc gccagcggcg gcgccatgta
     1741 gaccgattcg ttcttgctca gcatccgcca gtagccgccg tggatgaaga cgaaggccgg
     1801 cttcagcccg ggccccggcg ccggcacgat gtcgaggcac tggccgctcg cggcgtcgta
     1861 gcagaggtcg cgctcagtcg cgaacgcgtc gtagacggcg cgcgatcgtt cgcgatagag
     1921 cgccatgtgc gcgtcgaata cgccgggcgg cagcgaccgg ctcggtgaat acgccttgtc
     1981 gatcgccgga tcgctcgtga acgccatttc tcacactccc aggtagcgct gccagatcga
     2041 cgcatcggcg tcgagctcgg ccgaggagcc gctccaggcg acgcggcctt tctcgaggat
     2101 gacatggcgg tcggcgaggc ggatcagtct ctgcacgtat ttgtcgatca ccaggatcgc
     2161 ctgtccct
//
LOCUS       DASRWS010000694         6793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3147256, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000694 DASRWS010000000
VERSION     DASRWS010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147256"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1033)
                     /locus_tag="VFF72_13265"
     CDS             complement(272..1033)
                     /locus_tag="VFF72_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HZV94183.1"
                     /translation="MTHAALRDDARERAGDAIPVVGLIVPPAHGQVPGDGAALYGEDR
                     VRFIARGLALGEITTRGYEGVIDRVVGLAGELAGEGARAVSLMGTSLSFFRGAAFNAE
                     LEAEMARRTGVPCTTMSNAIVRALAAVDVARVAVATAYVDDVNERLCRYLAANDLAVT
                     AIRGLSITGVGEVAEVPTTTLVELCEAVFAVDRRADGILISCGGLLTLEAIAEVEAKL
                     GVPVVSSSPAGFWDVMRVAGLDSESRRGGRLFRSA"
     gene            complement(1030..2067)
                     /locus_tag="VFF72_13270"
     CDS             complement(1030..2067)
                     /locus_tag="VFF72_13270"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZV94184.1"
                     /translation="MANATSMTAPATLPAATSPRFAAVGAGRMGRSIAIAFAWAGYRI
                     SVIDLLSRDAPARAALHDDARGEIRASLLALAELGALPVTQVDQLAERIDLVASDGAA
                     AALAAAELVFEAVPERLDAKREAFAEIGRHCREDAILTSTTSSILVTQLALHVARPER
                     FLNVHWLNPAYLIPVVELSTHPATAPAVLERAKALFASIGKIPVVCGATPGYIVPRLQ
                     ALVMNEAARMVEEGVATAEEIDKATRYGLGFRFAALGVVEFIDFGGADILHHASREMS
                     ETIDAARYRAPEIIDRMVADGRLGLKTGSGFFDYAGRDAGAYRRDVMARTLGMLRHTG
                     LWRAAAKETLS"
     gene            complement(2080..3156)
                     /locus_tag="VFF72_13275"
     CDS             complement(2080..3156)
                     /locus_tag="VFF72_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HZV94185.1"
                     /translation="MKIAVLGGGHGSHAAAADLAEAGHEVRFWRRDAAALAPLRDEGS
                     IVLKDATGARAVPIALATTGIGEALRGAELVVIPLPATAQDDIARSLAPHLEDGQVVF
                     LPPGTFGSYAMAQIVREAGGTARVAWAETGTLPYLARKHGEREVNVTVRAVRLPTGVY
                     PARDADRAIEVIRRAFPSVHPCGDALSGALMNAGPVIHPPLMVMNAAPLQHFERWDIH
                     AEGTQPAVRAVTDALDRERIAVREALGYAAPHYPLADHYTGDRWMYGDAHQRLVRSGD
                     WREHIDLHTHRYITEDTELGLAFLASVARWAGVDAPIAHGLLAIVGGFLGRDLRRGPR
                     TLESMGLAGLDRATLALRLRDGEA"
     gene            complement(3241..4113)
                     /locus_tag="VFF72_13280"
     CDS             complement(3241..4113)
                     /locus_tag="VFF72_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZV94186.1"
                     /translation="MRKASPAAATARATRSAASAASGAQSVRRALAVLRLVATGQEAG
                     VRLVDIAEMSGLSRPTVHRLLKVLMSETAVEQDPQTRRYRVGPELSLLGLARGGRFAI
                     RAIAEPYLQALASEVGDTVFLSVRHGADSVCIGRYVGSHQIQVLSIEVGARRPLGASV
                     SGLILLAGMEPKEAKTITQANATRLALLARSPAQVLRDVAAAREQGHVAAPAGVMPGT
                     SALAVPVRDADGRVLAAISVAALADRLERRRWPAVLAQMQQQARRITRRHGEVGRARS
                     RIGGGDPDHGPPHD"
     gene            complement(4141..4761)
                     /locus_tag="VFF72_13285"
     CDS             complement(4141..4761)
                     /locus_tag="VFF72_13285"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZV94187.1"
                     /translation="MSLPATSLDPEATYRLLTGIVVPRPIAWVTTLSADGVVNLAPFS
                     CFTFVSNKPPLLGINVGRKAERRKDTGANIHHLGEFVVHIGDSTQMKAIHESSVEHAP
                     DVSEAELLGLRTVACDAVRVPRLADVPVSMECRLERVIAFGDTGSEFIVGEVLVFHIR
                     EGLLHNGKIDTRALDPVCRLGGPNYATLGPIVTLRPVAQTAKSVLG"
     gene            4969..5937
                     /locus_tag="VFF72_13290"
     CDS             4969..5937
                     /locus_tag="VFF72_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZV94188.1"
                     /translation="MKALWTAIAFAAIALSPVAPAEAAWPDKPIHLVVPYPPGGNVDS
                     AARIVSDRLGAALGQPFIVENKPGAGGMIAAEYVAKSPPDGYTFFMGANGPILFSPTI
                     FRKNLYDWKKDFIPIGSVSFTALVVQVSPSTPYKTFGELIAAGKKPDNTLTMASPGAG
                     TTNHLVSEYLQRESGAHWLTVHYKGNAPATTDLLGGHVQFDLDQISVALPFIKEGRTR
                     PLAVTSAKRLPQLPDVPTLQELGFKGFDALTFTGILAPAGTPADIIDRFSAALKKVLA
                     EKGVQDKFLGLGAEARFETPEQFTKYLAAEDARWIPVIRQSHITAE"
     gene            5940..6335
                     /locus_tag="VFF72_13295"
     CDS             5940..6335
                     /locus_tag="VFF72_13295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZV94189.1"
                     /translation="MAGRTSVYIDGFGHKNPIPAGCRIGPLLESGSIQGNDPATGKPA
                     ASIEAQCRFMLGNVKKIVEAAGGGVADIAKLTVWMKDRSQRPALNVPWLEMFPDAASR
                     PARHTMHAPELDMGKLVECSFTAWIDRDA"
     gene            6328..>6793
                     /locus_tag="VFF72_13300"
     CDS             6328..>6793
                     /locus_tag="VFF72_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV94190.1"
                     /translation="MPDYLPFDPNPRAPSPLPPPRSCDSQFHVFGPRETYPVRPNAAY
                     EMPSATWQVAQKLHATLGIERGVIVQATTYGADHQVVLDALAGLNAQGPRRYVACANA
                     LVLVERDDAYLQRLHDAGVRGARFTRGGLGISFSPAEQARAFARAKELGWYVK"
BASE COUNT         1041 a         2463 c         2372 g          917 t
ORIGIN      
        1 acaggccgcc gaccagccag caggcgacga aataggccgc gccgatcgca agccccgccc
       61 acggaccgat gtcgggcggc gcgaaataca tgcacgccga cgcataggca aggatgatcg
      121 cgtagccgat gacgttgtag atcctcaagc tgcacctcgt cgttctgttt tcgatgtcgg
      181 gccgcgcctt gccgcgaggc gcgagcttcg cgcggcagca tagcgcgaga cgcttcggga
      241 aaaggttcgc gcaaacgcgc gcgcgtcgac gtcaggcgct gcgaaagagc cttccgcccc
      301 gccgcgactc gctgtcgagc ccggccaccc gcatcacgtc ccagaagccc gccggcgagc
      361 tcgacacgac cgggacgccg agcttcgcct cgacctcggc gatcgcctcg agcgtcagca
      421 gcccgccgca cgagatgagg atgccgtcgg cgcggcggtc gacagcgaag accgcctcgc
      481 aaagctcgac cagcgtcgtc gtcggcacct cggccacctc gcccacgccg gtgatcgaca
      541 ggccgcggat ggcggtcacc gccaggtcgt tcgccgccag gtagcggcac agccgctcgt
      601 tgacgtcgtc gacgtacgcc gtcgccaccg cgacccgcgc gacatcgacg gcggcgaggg
      661 cccggacgat ggcgttgctc atggtcgtgc acggcacgcc ggtgcggcgc gccatctcgg
      721 cttcgagctc ggcgttgaac gcggcgccgc ggaaaaagct gagcgacgtg cccatcagcg
      781 agacggcgcg cgcaccttct cctgccaatt ccccggccag gccgacgacg cggtcgatca
      841 cgccttcgta gccgcgggtc gtgatctcgc cgagcgccag gccgcgggcg atgaagcgca
      901 cccggtcctc gccgtagagc gcggcgccgt cgccgggcac ctggccgtgc gccggtggca
      961 cgatcaggcc gacgacggga atggcgtcgc cggcgcgctc ccgggcgtcg tctcgcagtg
     1021 cggcgtgggt cacgacaagg tctccttcgc ggcggcacgc cacaggccgg tgtgcctgag
     1081 catgccgagg gtgcgcgcca tcacgtcgcg ccgataggcg ccggcatcgc gaccggcgta
     1141 gtcgaaaaag ccgctgccgg tcttcaggcc gagtcggccg tcggcgacca tgcggtcgat
     1201 gatctccggc gcgcggtagc gcgccgcgtc gatcgtctcc gacatctcgc ggctcgcgtg
     1261 gtgcaggatg tcggcgccgc cgaaatcgat gaactcgacg acgccgagcg cggcgaagcg
     1321 aaagcccagg ccgtagcgag tcgccttgtc gatctcctcg gccgtcgcca cgccctcctc
     1381 caccatgcgc gcggcctcgt tcatcaccag cgcctgcagg cgcggcacga tgtagcccgg
     1441 cgtcgcaccg cagacgaccg ggatcttgcc gatcgacgcg aagagcgcct tggcgcgttc
     1501 gagcacggcc ggtgccgtgg ccggatgcgt gctcagctcg acgaccggaa tcaggtacgc
     1561 cggattcagc cagtggacgt tgaggaagcg ctcgggccgc gcgacatgca gggccagctg
     1621 ggtgacgagg atgctgctcg tcgtcgaggt gaggatcgcg tcttcgcggc agtgacgccc
     1681 gatctcggcg aaggcttcgc gcttggcgtc gagccgctcg ggcaccgcct cgaagaccag
     1741 ctccgcagcg gccagcgccg ccgcggcgcc gtccgaggcg acgagatcga tgcgctcggc
     1801 cagctgatcc acctgcgtga ccggcagcgc gccgagctcg gcgagcgcca gcaggctggc
     1861 gcggatctcg ccgcgtgcgt cgtcgtgcaa tgccgcccgg gccggcgcgt cgcgcgagag
     1921 caggtcgatc acggaaatgc ggtagccggc ccaggcaaag gcgatggcga tgctgcggcc
     1981 catgcggccg gcaccgacgg cggcgaagcg cggcgacgtg gccgccggaa gggtcgcggg
     2041 cgcggtcatc gacgtggcgt tggccatggc agcgacgact caggcctcgc catcgcgaag
     2101 gcgcagcgcc agcgtcgcgc gatcgagccc ggccaggccc atcgactcga gcgtgcgcgg
     2161 cccccggcgc agatcgcgac cgagaaagcc gccgacgatc gccagcagcc cgtgcgcgat
     2221 cggcgcatcg accccggccc agcgcgccac cgaggcgagg aaggcgaggc cgagctcggt
     2281 gtcctccgtg atgtagcggt gcgtgtgcag gtcgatgtgc tcgcgccagt cacccgacct
     2341 gaccagccgc tggtgcgcgt cgccgtacat ccagcgatcg cccgtgtagt ggtcggcgag
     2401 cggatagtgc ggcgccgcgt agccgagcgc ttcgcgcacc gcgatgcgct cgcggtcgag
     2461 cgcgtcggtg acggcgcgca ccgccggctg cgtgccttcg gcgtggatgt cccagcgctc
     2521 gaaatgctgc agcggcgccg cgttcatcac catcagcggc ggatggatga ccgggccggc
     2581 gttcatcagc gcgcccgaaa gcgcgtcgcc gcacggatgg acgctcggaa aggcgcgacg
     2641 gatcacctcg atcgcgcggt cggcgtcgcg cgccgggtag acgccggtcg gcaggcggac
     2701 ggcgcggacc gtgacgttga cctcgcgctc gccgtgcttc ctcgccagat acggcagcgt
     2761 gccggtctcg gcccaggcga cgcgcgccgt gccgcccgcc tcgcgcacga tctgcgccat
     2821 cgcgtagctg ccgaaagtgc ccggcggcag gaagacgacc tggccgtcct cgagatgcgg
     2881 cgccagggac cgggcgatgt cgtcctgcgc ggtggccggc agcggaatga cgacgagctc
     2941 ggcgccgcgc agcgcttcgc cgatcccggt cgtcgccaag gcgatgggca ccgcacgcgc
     3001 gcccgtcgcg tctttcagga cgatgctgcc ctcgtcgcgc agcggcgcca gcgccgcggc
     3061 gtcgcgtcgc cagaagcgga cctcgtggcc cgcctccgcg aggtcggccg ccgctgcatg
     3121 actgccatgg ccaccgccga ggacggcgat cttcatggcg gcgaaggctt cgtggcggaa
     3181 atgggcatga cgcgcgatcg aaccgtggcg ggaagaagca tcgtagccgc gcggcgccga
     3241 tcagtcgtgc ggcggtccgt gatccggatc gccgccgccg atccgcgatc gggcccgacc
     3301 cacctcgcca tggcgacgcg tgatgcgccg cgcctgctgc tgcatctgcg ccagcaccgc
     3361 cggccagcgc ctgcgctcga ggcgatcggc gagcgcggcg acgctgatcg cggcgagcac
     3421 gcggccgtcg gcgtcgcgca ccggcaccgc cagcgcgctg gtgccgggca tcacgccggc
     3481 gggcgccgcg acgtgcccct gctcgcgcgc cgccgccacg tcgcgcagca cctgcgccgg
     3541 cgagcgcgcg agcagcgcca gccgggtcgc gttggcctgg gtgatcgtct tcgcctcctt
     3601 cggctccatg ccggcgagca ggatcaggcc cgagacactg gcaccgagcg gccggcgcgc
     3661 gccgacctcg atcgacagca cctggatctg gtggctgccg acgtagcgcc cgatgcacac
     3721 cgagtcggcg ccgtggcgga cgctgagaaa gaccgtgtcg ccgacttcgc tcgccagcgc
     3781 ctgcaggtac ggctcggcaa tggcgcggat cgcgaagcgg ccgccacgcg ccagtccgag
     3841 cagcgagagc tcggggccga cgcggtagcg gcgcgtctgc gggtcctgct cgaccgccgt
     3901 ctcgctcatc agcaccttca gcaggcgatg caccgtcgga cggctgaggc ccgacatttc
     3961 ggcgatgtcg acgaggcgga cgccggcttc ctggccggtc gcgacgagac gcagcacggc
     4021 cagggcgcgg cgcacgcttt gcgctcccga cgccgccgaa gcggccgacc gggtggcgcg
     4081 cgccgtcgcg gccgccggcg acgcctttcg cacggacggc gctgccttcg ccatcgccct
     4141 tcagccgagg accgacttgg ccgtctgcgc gacaggacgc aaggtgacga tcggccccag
     4201 ggtcgcgtag ttcggacccc cgagccggca caccgggtcg agcgcccgcg tgtcgatctt
     4261 gccgttgtgc agcaagcctt cgcgaatgtg gaacacgagc acttcaccga cgatgaactc
     4321 cgagcccgtg tcgccgaagg cgatgacgcg ctcgagccgg cattccatgc tcaccgggac
     4381 gtcggcgagc cgcggcacgc gcacggcgtc gcaggcgacg gtgcgcaggc cgagcagctc
     4441 ggcctcgctg acatcgggcg catgctcgac gctgctctcg tggatcgcct tcatctgcgt
     4501 gctgtcgccg atgtggacga cgaattcgcc gaggtgatgg atgttggcgc cggtgtcctt
     4561 gcgtcgctcg gccttgcggc cgacgttgat gcccaaaagc ggcggcttgt tggagacgaa
     4621 ggtgaagcac gagaacggcg cgaggttgac gacgccgtcg gccgagagcg tcgtcaccca
     4681 ggcgatgggc cgcggcacga cgatgccggt gaggagacgg taggtcgcct cgggatcgag
     4741 cgaggtggca ggcaacgaca tggcgaggat ctcgggcacg cgaagcgccg attgtggaca
     4801 gcgccggagc gcgaggcgat ccgacacgca gaaatagtcc gcatcgtgga acccggcggc
     4861 gttccggtgc cgggcagagg cctccctgcc gccgcttctc cctatagtcg tttcgtcccg
     4921 gcgaccggcc ctcggcgcag cggaccgacc ttcccaggag actcatccgt gaaagcactc
     4981 tggacggcga tcgcattcgc cgcgatcgca ctgtcgcccg tggcgccggc cgaggccgcc
     5041 tggcccgaca agccgatcca cctcgtcgtt ccctatcctc ccggcggcaa cgtcgacagc
     5101 gccgcgcgca tcgtcagcga ccgcctcggc gccgcgctcg gccagccctt catcgtcgag
     5161 aacaagccgg gcgccggcgg catgatcgcc gccgagtacg tagccaagtc gccgcccgac
     5221 ggctacacct tcttcatggg cgccaacggc ccgatcctgt tctcgccgac gatcttcagg
     5281 aagaacctgt acgactggaa gaaggacttc attccgatcg gctcggtgtc gttcacggcg
     5341 ctcgtcgtcc aggtgagccc gtcgacgccc tacaagacct tcggcgaact catcgccgcg
     5401 ggaaagaagc ccgacaacac gctgacgatg gcctcgcccg gcgccggcac gaccaaccac
     5461 ctggtgagcg aatacctgca acgcgaaagc ggcgcgcact ggctgaccgt gcactacaag
     5521 ggaaacgccc cggcgaccac cgacctgctc ggcggccacg tccagttcga cctcgaccag
     5581 atctcggtgg cgctgccctt catcaaggaa ggcaggacgc ggccgctggc ggtgacgtcg
     5641 gccaagcgtc tgccgcaact gcccgacgtg ccgacgttgc aggagctcgg cttcaagggc
     5701 ttcgatgccc tgaccttcac cggcatcctc gctccggccg gcacgccggc cgacatcatc
     5761 gatcgctttt cggccgccct gaagaaggtg ctcgccgaaa aaggcgtgca ggacaagttc
     5821 ctcggcctcg gcgccgaagc gcgcttcgag acgcccgagc agttcacgaa gtacctcgcc
     5881 gccgaggacg cgcgctggat cccggtgatc aggcaatcgc acatcacggc ggaatgacga
     5941 tggccgggcg aacgagcgtc tacatcgacg gcttcgggca caagaatccg attccggccg
     6001 gctgccgcat cggccccctc ctcgagagcg gcagcatcca gggcaacgac ccggccaccg
     6061 gcaagccggc cgcgtcgatc gaggcgcagt gccgcttcat gcttggcaac gtcaagaaga
     6121 tcgtcgaggc cgccggcggc ggcgtcgccg acatcgccaa gctcacggtc tggatgaagg
     6181 accgcagcca gcggcccgcg ctcaacgtgc cgtggctcga gatgtttccc gatgccgcgt
     6241 cgcggccggc ccggcacacg atgcacgcgc ccgagctcga catgggcaag cttgtcgagt
     6301 gcagcttcac cgcctggatc gaccgcgatg cctgactacc tgcccttcga tccgaacccg
     6361 cgcgcgccga gcccgttgcc gccgccgcgc tcgtgcgaca gccagttcca cgtcttcggc
     6421 ccgcgcgaaa cgtacccggt gcggccgaac gccgcctacg agatgccgag cgcgacctgg
     6481 caggtggcgc aaaagctgca cgccacgctc ggcatcgagc gcggcgtgat cgtccaggcc
     6541 accacctacg gcgccgatca ccaggtcgtc ctcgatgcgc tcgccggcct caatgcccag
     6601 ggcccgcgcc gctacgtcgc ctgtgccaat gcgctcgtgc tcgtcgagcg cgacgacgcc
     6661 tacctgcagc ggctccacga cgccggcgtg cgcggcgcgc gcttcacgcg cggcggcctg
     6721 ggcatcagct tctcgccggc cgagcaggcg cgcgccttcg cccgcgcgaa ggagctgggc
     6781 tggtacgtca agg
//
LOCUS       DASRWS010000695         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3147499, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000695 DASRWS010000000
VERSION     DASRWS010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147499"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1725)
                     /locus_tag="VFF72_13305"
     CDS             complement(34..1725)
                     /locus_tag="VFF72_13305"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZV94191.1"
                     /translation="MSALLSISDLRVAFRLGTDTGAVRRVEAVRGISFELAENSTLAL
                     VGESGSGKSVTAMSILDLLPDNAERSGAITFAGHDMLALPRAALQKLRGKEIACVFQD
                     PMSSLNPVFTVGQQIVEPLVKHLGMGRRTARKRAEELLAEVGMPEPRRRLAAYPHQLS
                     GGQQQRVMIAMALACEPRLLIADEPTTALDVTIQRQILELLARLKEARRMSMLFISHD
                     LGVVGEIADHVVVMRQGQVREQGGVEAIFENPQDAYTKALLACRPTLDQRSARLRVVD
                     DHVAGRATSPAGQAKDPNARVVVEASGVTKSFWIRRGVFGRSEFRAVKGASFRLRAGH
                     TLGIVGESGSGKTTLGLTLLRLHEANGGPVGGSVIFDGRDLLKMPRSELLAMRKRIQV
                     VFQNPYASLNPRFTIGQTLVEPMNIHDVGSGAADREARARDLLDRVGLDESAMQKYPH
                     EFSGGQRQRIAIARCLTLSPEVLVLDEAVSALDVSVQAQVLNLLKDLQDEFGFAYIFI
                     SHDLAVVRFMADEVLVMKDGVVVEQADVEQILERPQQEYTRRLLEAIPRGYRQAA"
     gene            complement(1722..>2458)
                     /locus_tag="VFF72_13310"
     CDS             complement(1722..>2458)
                     /locus_tag="VFF72_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZV94192.1"
                     /translation="EWAERAAKYKTVETPRAKTLPFGADRLGRDVLAKAMKGTQTSVF
                     VGLLAALVATVIGTFLGALAGFFGGKVGDFLEWVYNVFTSIPDILLILSFAVIFGRGI
                     ASVAIILALVGWTGLYRQVRAEFIKHSTRDYVRSAEAIGASAMSRMFRHILPNVGHVI
                     LVRMSLLVVAFIKFEVILSYLGLGVGVDDVSWGTMLAEAQSELILGYWWQLVAATAFM
                     AVFVTAFSLMTDAVRDALDPKLRGLE"
BASE COUNT          418 a          818 c          853 g          369 t
ORIGIN      
        1 accggcgatg acggatgacc agccgccggg cgatcaggcg gcctgccggt aaccgcgcgg
       61 gatcgcctcg agcaagcggc gcgtgtactc ctgctgcggc cgctcgagga tctgctcgac
      121 gtcggcctgc tcgacgacga cgccatcctt catcaccagc acctcgtcgg ccatgaagcg
      181 gacgaccgcg agatcgtggc tgatgaagat gtaggcgaag ccgaattcgt cctgcaggtc
      241 tttcagcagg ttgagcacct gcgcctgtac cgagacgtcg agcgcgctga cggcttcgtc
      301 gagcacgaga acctcgggcg agagcgtcag gcagcgggca atggcgatcc gctggcgctg
      361 gccgcccgag aactcgtgcg gatacttctg catcgccgat tcgtcgagcc cgacccggtc
      421 cagaaggtcg cgggcgcgcg cttcgcgatc ggcggcgccg ctgccgacgt cgtggatgtt
      481 catcggctcg accagcgtct ggccgatcgt gaatcgcgga ttgagcgaag cgtagggatt
      541 ctggaagacg acctggatgc gctttcgcat cgccagcaac tcggatcgcg gcatcttcag
      601 cagatcgcgt ccgtcgaaga tgacactgcc gccgaccggt ccgccgttgg cctcgtgcag
      661 gcgcagcaag gtcagcccga gcgtcgtctt gcccgacccc gactcgccga cgatgcccag
      721 ggtgtggccg gcgcgcaggc gaaagctcgc ccccttgacg gcacggaact cgctgcggcc
      781 gaagacgccg cgacggatcc agaagctctt cgtcacgccg ctcgcttcga cgacgacgcg
      841 ggcgttcgga tccttggcct ggccggccgg ggacgtggcg cgcccggcga cgtgatcgtc
      901 gacgacgcgc aaccgcgccg agcgctggtc gagcgtcggc cggcaggcca gcagggcctt
      961 cgtgtaggca tcctgcgggt tctcgaagat ggcttcgacg ccaccctgtt cgcgaacctg
     1021 gccctggcgc atgacgacca cgtgatcggc gatctcgccg acgacgccga ggtcgtggct
     1081 gatgaagagc atgctcatgc ggcgcgcttc cttcagccgc gccagcagct cgaggatctg
     1141 gcgctggatc gtgacgtcga gcgcggtcgt cggctcgtcg gcgatcagaa gccgcggctc
     1201 gcaggccagc gccatggcga tcatcacccg ctgctgctgg ccgcccgaga gctggtgcgg
     1261 atacgctgcc aggcgccgcc gcggctcggg catgcccacc tcggcgagca gctcttcggc
     1321 acgcttgcgc gcggtgcggc gccccatgcc gagatgcttg acaaggggct cgacgatctg
     1381 ctggcccacg gtgaagaccg gattcagcga gctcatcgga tcctggaaga cgcaggcgat
     1441 ctccttgccg cgcagcttct gcagcgcggc gcgcggcagc gccagcatgt cgtggccggc
     1501 gaaggtgatg gctcccgagc gttcggcgtt gtcgggcaga aggtcgagga tcgacatcgc
     1561 cgtcacgctc ttgccggagc cggattcgcc gaccagcgcg agcgtgctgt tctcggccag
     1621 ttcgaagctg atgccacgca cggcctcgac ccgccggacg gcgccggtgt cggtgccgag
     1681 tcgaaaggcg acccgcaaat cgctgatcga cagcagcgcg ctcactcgag cccccgaagc
     1741 ttcggatcga gggcgtcgcg caccgcgtcg gtcatcagcg agaaagccgt cacgaagacc
     1801 gccatgaacg ccgtggcggc gacgagctgc caccagtatc cgaggatgag ctcgctctgc
     1861 gcctcggcca gcatggtgcc ccacgagacg tcgtcgacac cgacgcccag gccgagatag
     1921 gagaggatca cctcgaactt gatgaacgcc accacgagca gcgacatccg caccaggatg
     1981 acgtggccga cgttgggcag gatgtgcctg aacatgcgcg acatcgccga ggcgccgatg
     2041 gcctcggccg agcggacgta gtcgcgcgtc gaatgcttga tgaactcggc gcgaacctgc
     2101 cggtagaggc cggtccagcc gaccagcgcc aggatgatgg cgacgctggc gatgccgcgt
     2161 ccgaagatga ccgcgaacga caggatgagc aggatgtcgg ggatcgaggt gaagacgttg
     2221 tagacccact cgagaaagtc gccgaccttg ccgccgaaga acccggccaa ggcgccgagg
     2281 aaggtgccga tcacggtggc gacgagggcg gcgagcaggc ccacgaagac cgaggtctgg
     2341 gtgcccttca tcgccttggc gagaacgtcg cggccgagcc ggtcggcgcc gaaaggcaag
     2401 gtcttcgcgc gcggcgtctc gacggtcttg tacttcgcgg cgcgctcggc ccattcct
//
LOCUS       DASRWS010000696         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1993177, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000696 DASRWS010000000
VERSION     DASRWS010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993177"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VFF72_13315"
     CDS             <1..153
                     /locus_tag="VFF72_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV94193.1"
                     /translation="KLLVAEDIPLEALQSKDDKVAVPPLVGALLKNEQDVSLFEKLVF
                     MQKHNR"
     gene            161..754
                     /locus_tag="VFF72_13320"
     CDS             161..754
                     /locus_tag="VFF72_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV94194.1"
                     /translation="MKTVQPVTLADARVRLEPLGLQHVDGLKLAAADGELWKVRVTSV
                     PDPDDTTGYVERALQGFADGHRLAFAVIDVASGAVIGSSSYHDIVPAIERLEIGYTWY
                     AKSRQRTHVNASAKLLLMTHAFETLGAKLLGWRTDNFNFTSQRAIERLGARKDGVLRH
                     HAPRRDGTVRDTVMYSMTPDEWPGAKAELLARLGRRG"
     gene            841..>2243
                     /locus_tag="VFF72_13325"
     CDS             841..>2243
                     /locus_tag="VFF72_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZV94195.1"
                     /translation="MAPTPTFTTNNDDTTRPMAEHKGKPLDIETLWKIERLGAIALSP
                     DGAQAVCSLTTFSMAENSAAASLWLLSTFGGEPRRLTACGEKDGQAAWSPQGGAIAFV
                     ARREQEGKKDETAQLYLIASDGGEARRISDFAPGIEAFKWFPDGRRIAFVAWVWPELK
                     GTKAQGRRFKEWKERKETAYVTSEAQYRYWDRNLPMGRVAHLHVLDIASGRIVDLFEG
                     SPFELQRMDPDSSVFDVSADGRDIVFAFDPAAEKGLSNCRALVEIDVRSGRFATIAED
                     AEWDFETPRYSPDGSHIAFLASAIGRRHTALAHLAMLERGGTWRALTAAWDHGVVAPL
                     QWSADGSALYFTAEERGRRHLWRYALQSGSAGMAAEGGWVHHFDVAGDTVATVADSIE
                     HPARVHVRRPGERALRIERFNDELLAGTRFGASTEVMFAGAAGEQVQMWLVTPPGFDA
                     KKAKKWPVLHNIHGGPHS"
BASE COUNT          376 a          803 c          761 g          303 t
ORIGIN      
        1 aagctcctcg tcgccgagga catcccgctc gaagcgctgc agagcaagga cgacaaggtc
       61 gccgtgccgc cgctggtcgg cgcgctgctg aagaacgagc aggacgtctc gctcttcgaa
      121 aagctcgtct tcatgcagaa gcacaaccgc tgaggccgcc atgaagacgg tgcagccggt
      181 caccctcgcc gatgcccgcg tgcggctcga gcccctgggc ttgcagcacg tcgatggcct
      241 caagctcgcc gccgccgacg gcgagctctg gaaggtccgc gtcaccagcg tgcccgaccc
      301 ggacgacacg acaggctacg tcgagcgcgc cctgcaggga ttcgccgacg gccaccggct
      361 cgccttcgcc gtgatcgacg tggcgagcgg cgcggtcatc ggctcgtcga gctaccacga
      421 catcgtgccg gccatcgagc gcctagagat cggctacacg tggtacgcga agagccggca
      481 gcgcacccac gtcaacgcct cggccaagct gctcctcatg acgcacgcct tcgagacgct
      541 cggcgcgaag ctcctcggct ggcgcaccga caacttcaac ttcacgagcc agcgcgccat
      601 cgagcgcctc ggcgcccgca aggacggcgt cctccgccac catgcaccac gtcgcgacgg
      661 cacggtgcgc gacacggtga tgtacagcat gacgccagac gagtggccgg gagcgaaagc
      721 cgagcttctc gcccgcctcg gtcgccgcgg ctgaaccggg gcagggcctg gcgtcgcccg
      781 gggccgggcg cacaatccgc gccagcttcg agcgcggtga cgagaccttc gcgggcgagc
      841 atggccccga ccccgacgtt cacgacgaac aacgacgaca cgacgcgacc catggccgag
      901 cacaagggca agccgctcga catcgagacc ctgtggaaga tcgagcgcct cggcgcgatc
      961 gctctttcgc ccgacggcgc ccaggccgtc tgctcgctca cgaccttctc gatggcggag
     1021 aacagcgcag cggcgagcct gtggctgctc tcgaccttcg gcggtgagcc gcgccgtctc
     1081 acggcgtgcg gcgagaagga cggccaggcc gcgtggtcgc cgcagggcgg cgccatcgcc
     1141 ttcgtcgccc gccgcgagca ggaaggaaag aaggacgaga cggcgcagct ctacctgatc
     1201 gcgtccgacg gcggcgaagc ccgcaggatc agcgatttcg ctcccggcat cgaggccttc
     1261 aagtggttcc ccgacggcag gcggatcgcc ttcgtcgcgt gggtctggcc cgagctgaag
     1321 ggaaccaagg cgcagggcag gcgcttcaag gaatggaagg aacgcaagga aacggcctac
     1381 gtcacgtccg aggcgcagta ccgctactgg gaccgcaacc tgccgatggg gcgcgtcgcc
     1441 cacttgcatg tcctcgacat cgccagcggc cgcatcgtcg acctgttcga gggcagtccg
     1501 ttcgagctgc aacgcatgga ccccgactcg agcgtcttcg acgtctcggc ggacggccgc
     1561 gacatcgtct tcgccttcga tccggctgcc gagaagggtc tctccaattg ccgggcgctg
     1621 gtcgagatcg acgtgcgcag cggccgcttc gcgacgatcg ccgaagacgc cgagtgggac
     1681 ttcgagacgc cgcgctacag ccccgacggc tcccacatcg ccttcctggc cagcgccatc
     1741 ggccggcgtc acaccgcgct cgcccacctg gccatgctcg aacgcggcgg cacctggcgg
     1801 gcgctgacgg cggcgtggga ccacggcgtc gtcgcgccgc tgcagtggtc ggccgacggc
     1861 agcgcgctct atttcaccgc cgaggagcgc ggccgccggc acctctggcg ctatgcgctg
     1921 caaagcggca gcgccggcat ggcggccgag gggggctggg tccaccactt cgacgtcgcc
     1981 ggcgacacgg tcgcgaccgt cgccgattcg atcgagcatc cggcccgcgt ccacgtgcga
     2041 aggccgggcg aacgcgcgtt gcggatcgag cgtttcaacg acgagctgct cgccggcact
     2101 cgattcggcg cgtcgaccga ggtgatgttc gccggcgcgg cgggcgaaca ggtgcagatg
     2161 tggctcgtca cgccgcccgg cttcgatgcg aagaaggcga agaagtggcc ggtgctgcac
     2221 aacatccacg gcggcccgca ttc
//
LOCUS       DASRWS010000697         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3148590, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000697 DASRWS010000000
VERSION     DASRWS010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148590"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VFF72_13330"
     CDS             <1..1551
                     /locus_tag="VFF72_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZV94196.1"
                     /translation="EYAIFMLDVNGRVATWNVGAERIKGYKANEIIGRHFSVFYPPEV
                     RQSGWPEHELQVAADVGSFTDEGWRLRKDGTPFWAHVTITALRDEKKQLLGFAKLTRD
                     QSERKRSEAAEVLRDEREEILEAERSARMAAQRAVRIKDEFLATLSHELRTPLSAILG
                     WTQVLLRSAEIGSGEKTRHAIEVIDRNARAQVQLIDDLLDLSRLMTGKIRLDLRQVAV
                     ADLVQSAVDSVWPAAEAKGIALEAELDAGPATLSADSGRLQQVMSNLLGNAVKFTPRG
                     GRVRVALQTLGSRVELSVSDTGIGIPADFLPRVFDRFSQRDSSTTRAFGGLGLGLAIS
                     RQLVELHGGTITVASPGEGQGATFVVELPLSTPQTEDDNADHPQPTGQTASGEVLALP
                     RLEGIHAFIVDDEQDAREVLRRVLEDQGARVTAFSSAEDVLATLETSRPSILISDVGM
                     PGMDGYQLIRALRAAHPGDRLPALALTAFARAEDRKRSLVAGYQAHLAKPFDVGELIL
                     VVAGMLGK"
     gene            complement(1651..2847)
                     /locus_tag="VFF72_13335"
     CDS             complement(1651..2847)
                     /locus_tag="VFF72_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HZV94197.1"
                     /translation="MKHPFDLSTTRFDAKAISATIARALASAGLDPTSGPMRNVTGVI
                     ERALSSGVARESTVASSADDAVIDVAARVVGDVGDALPDADESSARVDPGAPGEFETH
                     RFTSAAGSRAYRLYVPRQIGQTPAAMVVMLHGCTQSASDFAAGTRMNRLADEHGFLVL
                     YPEQAANANASRCWNWFQEQDQLRDSGEPSLIAAMVREVAASHGADSRRIFVAGLSAG
                     AAMAVILGRTYPDLFAGVGAHSGLPYASAHDVASAMAAMKGGRGLAGLQRTPGASQSP
                     GARRVARAVPTIVFHGDRDHTVQQSNGAEIVQQASNAYANGNPTTSLRVSARGPASEN
                     GRSFSRTDHVDDAGRHLIEEWTLHGAGHAWSGGDASGSFTDSAGPDASAKMVEFFLSL
                     PRAGIA"
     gene            complement(2874..>3159)
                     /locus_tag="VFF72_13340"
     CDS             complement(2874..>3159)
                     /locus_tag="VFF72_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZV94198.1"
                     /translation="IRTALRNQIALHSDTLKQTVARRTLTVGLQHYSRADLERAVAAK
                     QPLDVQVVGLARIADDVTPKLAMAAIASVTVLGALQASPAVRQALSDRIR"
BASE COUNT          502 a         1061 c         1121 g          475 t
ORIGIN      
        1 gaatacgcga tcttcatgct cgacgtgaac ggcagggtgg cgacgtggaa cgtcggcgcc
       61 gaacggatca agggctacaa ggcgaacgaa atcatcggcc ggcacttctc ggtcttctac
      121 ccgcccgaag tgaggcaaag cggctggccc gagcacgagc tgcaagtcgc cgccgacgtc
      181 ggcagcttca ccgacgaggg atggcgcctg cgcaaggacg gaacgccgtt ctgggcccac
      241 gtcaccatca ccgcgctgcg cgacgagaaa aagcagctgc tcgggttcgc caagctgacc
      301 cgcgaccaga gcgaacgcaa gcgcagcgag gccgccgagg ttttgaggga tgagcgcgaa
      361 gagatcctgg aggcggagcg aagcgcgcgc atggccgcgc aacgcgccgt ccgcatcaag
      421 gacgagttcc tcgccacgct ttcgcacgag ctgcggacgc cgctcagcgc catcctcggc
      481 tggacgcagg tgctcctgcg ttcggccgag atcggctcgg gtgagaagac gaggcacgcc
      541 atcgaagtca tcgaccgcaa cgcacgcgcc caggtgcagc tcatcgacga tcttctcgac
      601 ctgagccggt tgatgaccgg caagatccgg ctcgatctgc gccaggtcgc cgtcgccgac
      661 ctcgtccagt cggcggtcga ttcggtctgg cctgccgccg aggcgaaggg catcgcgctc
      721 gaggccgagc tcgacgccgg cccggcgacg ctcagcgccg acagcggccg cctccagcag
      781 gtgatgtcga acctgctcgg caatgcggtc aagttcaccc cccggggcgg ccgggtccgg
      841 gtcgcgttgc agacgctcgg ctcccgcgtc gagctgagcg tgagcgatac cggcatcggc
      901 attccggccg acttcctgcc ccgcgtcttc gaccgctttt cccagcgcga cagctcgacg
      961 acacgcgcgt tcggcggcct cggcctcggc ttggcgatct ccaggcagct cgtcgagctg
     1021 catggcggaa cgatcaccgt ggcgagcccg ggcgagggcc agggcgcgac cttcgtggtc
     1081 gagctgccgc tatcgacccc gcagaccgag gacgacaacg cggatcaccc gcagccgacc
     1141 gggcagaccg cgtcgggcga agtgctcgcc ctgcccaggc tcgaaggcat ccacgctttc
     1201 atcgtcgatg acgagcagga cgccagagag gtccttcgcc gggtcctgga agaccagggg
     1261 gcgcgggtca cggccttcag ctcggccgaa gacgtgctgg ccacgctgga gacgtcgagg
     1321 ccgtcgatcc tcatcagcga cgtcggcatg ccggggatgg atggctacca gctcattcgc
     1381 gcgctgcgtg cggcccaccc cggcgaccgc cttcctgcac tggcactgac cgcattcgcg
     1441 cgcgccgagg accgcaagcg ctcgctggtc gccggatacc aggcgcatct cgccaagccc
     1501 ttcgatgtcg gcgagctgat cctggtcgtc gccggcatgc tcgggaagtg aaccgctgcc
     1561 ggctgccggc cgacgaacgt ggcgacggcg gagtcgtgcc cggcgtgcga gctcggcgat
     1621 gcggcggttt cccggaccgg caagccgcct tcaggcgatt ccggcgcgag gcagggacag
     1681 gaagaactcg accatcttcg ccgatgcatc ggggccggcg ctgtcggtga acgagcccga
     1741 cgcatcgccg cccgaccagg catggccggc gccgtgcaag gtccactctt cgatcaggtg
     1801 ccgcccggcg tcatcgacat ggtcggtgcg gctgaagctg cgaccgtttt cggacgccgg
     1861 tccacgcgcg ctgacgcgca acgaggtggt cgggtttcca ttggcgtagg cattgcttgc
     1921 ctgctgcacg atctcggccc cgttcgattg ctgcaccgtg tggtcgcgat cgccgtggaa
     1981 gacgatggtc ggtaccgcac gggcgacgcg tcgggcaccg ggcgattgcg acgcgccggg
     2041 agtccgttga aggccggcta ggccgcggcc gcccttcatc gccgccattg ccgacgcgac
     2101 gtcgtgggcg ctcgcgtagg gcaggcccga gtgcgcgccg acgccggcga acagatcggg
     2161 atacgtccgg ccgaggatga cggccatcgc cgcgcccgcc gacaggccgg cgacgaagat
     2221 gcgacgcgag tccgcgccgt ggctcgcggc gacctcgcgc accatggcgg cgatcaggga
     2281 aggctctccc gagtcgcgga gctggtcctg ttcctggaac cagttccagc atctcgaagc
     2341 gttcgcgttg gcggcctgct cggggtagag aaccaagaag ccgtgctcgt cggccaggcg
     2401 gttcatgcgc gtgccggctg cgaaatcgct ggccgactgg gtgcagccgt gcagcatcac
     2461 gaccatggcc gctggcgtct gcccgatctg ccgaggcacg tagagcctgt aggcacggct
     2521 gccggcagcg gaggtgaacc gatgcgtctc gaattcgcca ggcgcacccg gatcgacgcg
     2581 cgcactgctt tcatccgcgt cggggagcgc gtcgccgacg tcgcccacga cgcgagccgc
     2641 gacgtcgatc acggcgtcgt ctgcggaaga ggcgacggtc gattccctgg cgacgcccga
     2701 agacaacgca cgctcgatga caccggtcac gttgcgcatc gggccggatg tcggatcgag
     2761 gcctgccgac gcgagcgcgc gcgcgatcgt cgcgctgatg gccttggcgt cgaagcgcgt
     2821 ggtggagaga tcgaaagggt gcttcatgaa actcctggtt gttcttgcgg ggtctagcgg
     2881 attcgatcgg acaaggcttg ccgcacggcg gggctggcct gcaaggcgcc cagcacggtc
     2941 accgaggcga tcgctgccat cgccagcttg ggcgtcacgt cgtcggcgat gcgggccagg
     3001 ccgacgacct gcacgtcgag tggctgcttc gcggcgaccg cgcgctcgag atcggcgcgg
     3061 ctgtaatgtt gcaggccgac ggtcagggtg cgccgggcca cggtctgctt gagggtgtcg
     3121 gaatgcaggg cgatctggtt gcgaagggcg gtcctgatc
//
LOCUS       DASRWS010000698         4148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3737594, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000698 DASRWS010000000
VERSION     DASRWS010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737594"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VFF72_13345"
     CDS             <1..112
                     /locus_tag="VFF72_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZV94199.1"
                     /translation="TIGIEGQAFAARLQSPEAKEAFSAFFQKRKPDFSRF"
     gene            69..878
                     /locus_tag="VFF72_13350"
     CDS             69..878
                     /locus_tag="VFF72_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZV94200.1"
                     /translation="MPSSRSASPISPASEEAPLPSAAAALATKIVLAPLLLTQALRTR
                     RLAQRLPEADGAREGCTEGDPGASLRLLIAGDSSAAGVGVAQQDDAVVGHLVRALHRQ
                     CGRRIAWTLRARTGLTTRAVHELLRSEPPAPVDFAVVISGVNDVTGGVGVRRALGHRT
                     ALADWLLGANLARHVVFAPLPPLHRFPLLPEPLRRVLGDDARRHDLALARWTQDRTDV
                     SHAGFRVDLHPGVMASDGFHPGEPVYRACGDALAAHVACRLSAQSARLRDD"
     gene            1074..1904
                     /locus_tag="VFF72_13355"
     CDS             1074..1904
                     /locus_tag="VFF72_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZV94201.1"
                     /translation="MTLAGKTLFITGASRGIGLAIALRAARDGARIAIAAKTAEANPK
                     LPGTIFSAAAGIEAAGGEALPLQCDIRDEASVLAAVEATVQRFGGIDILVNNASAISL
                     TPTPATPMKRFDLMFGVNVRGTFACTQACLPHLAKSAAAGRNAHVLNMSPPLSMREHW
                     FAPHTAYTMAKYGMSMCTLGHAGEFRPLGIAVNSLWPRTAIKTAALQMIPGVDLDACR
                     TPEILADAAWLILTSDAKTTSGNFFIDDALLAERGVTDLDRYSVKPGTTRFVPDFFVD
                     "
     gene            complement(1961..2389)
                     /locus_tag="VFF72_13360"
     CDS             complement(1961..2389)
                     /locus_tag="VFF72_13360"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZV94202.1"
                     /translation="MPQIEREADLSSIAPLTLPDDRQLVMRVMPMPADANGNGDIFGG
                     WIMAQVDIAGAVLPARIAKGRIATVAVNQFVFKQPVSMGDLLSFYAKVERIGRTSVTV
                     NVEVYAERNPANLHVVKVTEANLTYVAIDGSGRPRPIPLD"
     gene            2458..>4148
                     /locus_tag="VFF72_13365"
     CDS             2458..>4148
                     /locus_tag="VFF72_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZV94203.1"
                     /translation="MRPATLSVPPPAASPGRPAAAGSDWRVLGKLLPYVWRWRWRVLV
                     ALACLVAAKLANIGVPLVLKHLVDALTIKPGDPRAVLVVPVALLVGYGALRLSITAFT
                     ELREFLFYPVAARIARRVSLEAFEHLLGLSLRFHLERQTGGVSRDIERGSRSIQSLLN
                     YTIYNILPTLVEISMVIVLLAAKFDGWFAAITFGALLVYIGFTVTLTEWRTAFRRAMN
                     EQDSQANTKALDALLNYETVKYFSNEPFEQARYDANLQALEKASVKSQASLSLLNLGQ
                     SLIIAIAVTLLVWRATVGVVDKEMTLGDLVLVNALMIQLYIPLNFLGVIYREIKQSMI
                     DMEKMFTLLGQGREIADAPNAQPLALHRSATASPCGSVRFESVRFGYDADREILHGVD
                     FEIPAGKTVAVVGPSGSGKSTLARLMFRFYDVDAGRISVDGQDIRDVTQKSLREAIGI
                     VPQDTVLFNDTVEYNIAYGRTGASRAEVEAAARSAHIHAFIASTPRGYDTMVGERGLK
                     LSGGEKQRVAIARTLLKNPPIMIFDEATSALDSANERAIQAELQSAARNRTTLVIA"
BASE COUNT          633 a         1487 c         1352 g          676 t
ORIGIN      
        1 gacgatcggc atcgagggcc aggcttttgc ggcgcgcctg cagagtccgg aagcgaagga
       61 agccttcagt gccttcttcc agaagcgcaa gcccgatttc tcccgcttct gaagaagcgc
      121 cgctcccttc ggcggcggcc gcgctggcga cgaagatcgt cctggcgccg ctgttgctga
      181 cgcaggcctt gcgaacccgg cgactggcac agcgccttcc cgaggcggac ggtgcgcgcg
      241 agggatgcac cgagggcgat ccgggcgcgt cccttcgatt gctgattgcc ggcgactcgt
      301 cggccgccgg cgtcggcgtc gcgcagcagg acgacgccgt cgtcggtcac ctggttcgcg
      361 ccctgcatcg gcagtgcggt cgcaggatcg cctggacatt gcgcgcccgc accgggctga
      421 cgactcgcgc ggtgcatgaa ctcctgcgca gcgagccgcc ggcgccggtg gacttcgcag
      481 tggtcatcag cggcgtcaac gatgtcaccg gcggcgtcgg cgtgcgtcgg gcgctcggcc
      541 atcggaccgc gctggccgac tggctgctcg gcgcgaacct cgcacgccac gtcgtgttcg
      601 cgcccttgcc gccgctgcac cgctttcctc tcctgcccga accgctgcgc cgggtgctgg
      661 gcgacgatgc gcgtcgtcac gatctcgccc tggcccgctg gacgcaggac cgcaccgacg
      721 tgtcgcacgc cgggtttcgc gtcgacctcc atcccggcgt catggcaagc gacgggttcc
      781 acccgggcga gccggtctac cgcgcctgcg gcgacgcgct ggccgcgcac gtcgcgtgcc
      841 ggttgtcggc acagtcggct cgccttcgtg acgattgaga cgacgcgcgc gacacgccat
      901 tgcggcgcgt cattgatgca cgctttcccg tgcttcaccg caggacgatc accgcgcaga
      961 ctgccaacca cgcggatcgg cagccccgcg ccgccaaccg cacggtccgg aacctccctg
     1021 gccacgccga tcacgccatc ccgcccatca cccagacaga ggagcgaacg accatgactc
     1081 tcgccggcaa gaccttgttc atcaccggtg catcgcgcgg catcggcctg gccatcgccc
     1141 tgcgtgccgc gcgcgacggc gcccgcatcg ccatcgccgc gaagaccgcc gaggcgaatc
     1201 cgaaactgcc ggggacgatc ttcagcgccg ccgccgggat cgaggccgcg ggcggcgagg
     1261 cgttgcctct gcaatgcgac attcgcgacg aggcgagtgt gctcgccgcc gtcgaggcga
     1321 cggtacagcg cttcggcggc atcgacatcc tcgtcaacaa cgccagcgcg atcagcctga
     1381 cgccgacccc ggcgacgccg atgaagcgct tcgacctgat gttcggcgtc aacgtgcgcg
     1441 gcacgttcgc ctgcacccag gcctgcttgc cgcatctcgc gaaatccgcc gcggccgggc
     1501 gcaacgcgca cgttctcaac atgtcgccgc cgctgtcgat gcgcgagcac tggttcgcgc
     1561 cgcacaccgc ctacacgatg gccaagtacg gcatgagcat gtgcacgctc ggccatgccg
     1621 gtgaattccg gccgctcggc atcgccgtca acagcctctg gccgcgcacg gcgatcaaga
     1681 cggcggcgct gcagatgatc ccgggcgtcg acctcgacgc ctgccgcacg cccgagatcc
     1741 tggccgatgc cgcgtggctc atcctcacca gcgacgcgaa gacgaccagc ggcaacttct
     1801 tcatcgacga cgcgctgctc gccgagcgtg gcgtgaccga cctcgatcgc tacagcgtca
     1861 agccgggaac gacgcgattc gttcccgact tcttcgtcga ttgacgcgac cgcaatcgcg
     1921 aggcgcgacc cgcgcacgac gcgccgtcgg cgggcccggc tcagtcgagg ggtatcggcc
     1981 gcggccgccc cgagccgtcg atcgccacgt aggtgagatt ggcctcggtc accttgacga
     2041 cgtgcaggtt ggccgggttg cgctcggcgt agacctcgac gttgaccgtc accgacgtcc
     2101 ggccgatccg ctcgaccttc gcatagaagc tcagcaggtc gcccatcgag accggctgct
     2161 tgaagacgaa ctggttgacc gcgacggtgg cgattcggcc cttggcgatc ctggccggaa
     2221 ggacggcgcc ggcgatgtcg acctgggcca tgatccagcc tccgaagatg tcgccgttgc
     2281 cgttggcatc ggcgggcatc ggcatcaccc gcatcacgag ctgccggtcg tcgggcaagg
     2341 tcagtggcgc gatcgaggaa aggtcggctt cgcgctcgat ctggggcata ttggcgcagg
     2401 tcgcggtgca gggaccttcg attctttcat cattcatccg gccgagccgg cgatttcatg
     2461 cgacccgcta ccctttccgt tcctcctcct gccgcgtcgc cgggccgtcc tgccgcggcc
     2521 ggatccgact ggcgagtcct cggcaagctg ctgccctatg tctggcgctg gcgctggcgc
     2581 gtgctggtgg cgctcgcctg cctggtggcc gccaagctcg ccaacatcgg cgtgccgctg
     2641 gtcctcaagc acctcgtcga cgcgttgacg atcaagcccg gcgacccgcg cgcggtgctg
     2701 gtcgtcccgg tggccctgct cgtcggctac ggcgcgcttc gcctgtcgat caccgccttc
     2761 accgagctgc gcgaattcct cttctacccg gtggcggcgc gcatcgccag gcgcgtctcg
     2821 ctggaagcgt tcgagcacct gctgggcctg tcgctgcgct tccacctcga gaggcagaca
     2881 ggtggcgtct cgcgcgacat cgagcgcggc tcgcgctcga tccagtcgct tctcaactac
     2941 acgatctaca acatcctgcc gacgctggtc gagatctcga tggtgatcgt gctgctggcc
     3001 gcgaagttcg acggctggtt cgcggccatc acgttcggcg cgctgctggt ctacatcggc
     3061 ttcacggtga cgttgacgga atggcgcacg gcctttcgcc gcgccatgaa cgagcaggac
     3121 agccaggcca acaccaaggc gctcgacgcg cttctcaact acgagacggt gaagtacttc
     3181 tcgaacgagc cgttcgagca ggcccgctac gacgccaacc tgcaggcgct cgagaaggcc
     3241 tcggtgaagt cgcaggcttc gctgtcgctg ctcaacctcg gccagagcct gatcatcgcg
     3301 atcgcggtga cgctgctcgt ctggcgcgcg acggtgggcg tcgtcgacaa ggaaatgacg
     3361 ctcggcgacc tcgtgctcgt caacgcgctg atgatccagc tctacatccc gctcaatttc
     3421 ctcggcgtca tctaccgcga gatcaagcag tcgatgatcg acatggagaa gatgttcacc
     3481 ctgctcggcc agggccgcga gatcgccgat gcgccgaacg cgcagccgct ggcgctgcac
     3541 aggagcgcga cggcttcgcc ctgcggctcg gtccgcttcg agagcgtccg cttcggctac
     3601 gacgccgatc gcgagatcct gcacggcgtc gacttcgaga tccccgccgg caagacggtc
     3661 gcggtggtcg gcccctcggg gtcgggcaag agcacgctgg cccgcctgat gtttcgcttc
     3721 tacgacgtcg atgccggccg catctcggtc gacgggcagg acatccgcga cgtcacccag
     3781 aagagccttc gcgaggcgat cggcatcgtt ccgcaggaca ccgtgctctt caacgacacg
     3841 gtcgaataca acatcgccta cggccgcacc ggcgccagcc gggccgaggt cgaggccgcg
     3901 gcgcggtcgg ctcatatcca cgccttcatc gcctcgacgc cgcgcggcta cgacacgatg
     3961 gtgggcgagc gcggcctgaa gctctcgggc ggcgagaagc agcgcgtcgc gatcgcgcgc
     4021 acgcttttga agaatccgcc gatcatgatc ttcgacgagg cgacctcggc gctcgattcg
     4081 gccaacgagc gggcgatcca ggccgagctg cagagcgcgg cgagaaaccg cacgacactg
     4141 gtcatcgc
//
LOCUS       DASRWS010000699         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1994696, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000699 DASRWS010000000
VERSION     DASRWS010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994696"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VFF72_13370"
     CDS             <1..1374
                     /locus_tag="VFF72_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZV94204.1"
                     /translation="VQVLELEDEAGGNSRGHTMGGMRCPLGAHYLPLPGAPAVEVIEL
                     LDELGLRRIVHGRVEYDELMLCASPQERLFIGGGWRDGLLPPIEALPAGERSATQAHY
                     RAFAAAIGELGRDASFTIPTARSRWSPALTALDAMTFDAWLDGRGLKSTALRWHLDYC
                     CRDDYGAGSAQVSAWAGVHYFASRHGFHAPGEESADAGDGLLTWPEGNAWLAERLAAP
                     LGERLRAGRVALRVQEGRDDVTVDVWDAVAGRRERWIATQAIIATPLFIAARLFDAPP
                     PALAEAAPLVRHAPWLVANLQLDSALDDSPGAPLSWDNVIYRGETLGYVDAMHQSTMP
                     FAGATVLSAYWALGGEGDAMLRTQRRRLLDDDWRAWAARIVADLSGPHPDLAGRLKRA
                     DLMRYGHAMAIPAPGVRSHPALRALAETTGRVHFAHADLSAYSVFEEAFFHGTRAGRA
                     AAATAIG"
     gene            1594..1770
                     /locus_tag="VFF72_13375"
     CDS             1594..1770
                     /locus_tag="VFF72_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94205.1"
                     /translation="MILLRLLIATIALAVAGVANAAPASPESVARLLEVTRVRQQLEV
                     MRTQSLPRMLDAVR"
     gene            1822..2097
                     /locus_tag="VFF72_13380"
     CDS             1822..2097
                     /locus_tag="VFF72_13380"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HZV94206.1"
                     /translation="MQPRFLAIMNEALDWDTMRPQYVAMYVDTFSQEEVDGLIAFYQS
                     PLGTALLDKMPLLIQRSMQLAQQRMLPLLQKIEEMAKEEATTSGGKP"
     gene            2115..>2827
                     /locus_tag="VFF72_13385"
     CDS             2115..>2827
                     /locus_tag="VFF72_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZV94207.1"
                     /translation="MRTPRFNVAIERNAFLVLLVLLTIGFAWIVWPFYGAVFWAVVLA
                     VLFEPLNRHVLRRFKGRSTLAALATLAVILVIVILPTAFVAMSLVQEIASLYQRVRSG
                     DANLATYFDQFSAALPSWASHLMDKLGFGDLAGVQGTLTAAITQRGEALAARAVNVGQ
                     DTVDFMLGFGLSMYILFFLLRDGPALAAKVRGALPLAPEPKARLLERFTTVIRATVKG
                     NILVAAAQGALGGLAFWVL"
BASE COUNT          407 a         1036 c          929 g          455 t
ORIGIN      
        1 gtgcaggtgc tcgagctcga ggacgaagcc ggcggcaaca gtcgcggcca cacgatgggc
       61 ggcatgcgct gtccgctggg cgcccactac ctgccgctgc cgggcgcacc ggcggtcgag
      121 gtcatcgagc ttctcgacga gctcggcctg cgtcgcatcg tccatggccg cgtcgaatac
      181 gacgagctca tgctctgcgc cagtccgcag gagcggctct tcatcggcgg cggctggcgc
      241 gatggcctgc tgccgccgat cgaggcgctc cccgccggcg aacgctcggc gacccaggcc
      301 cactaccgcg ccttcgcggc agcgatcggc gagctcggcc gcgacgcttc cttcacgatc
      361 ccgacggcgc gctcgcgctg gtcgcctgcg ctgacagcgc tcgatgcgat gacgttcgat
      421 gcctggctcg atgggcgagg cctcaagtcg acggcgttgc gctggcatct cgactactgc
      481 tgtcgcgacg actacggcgc cggcagcgcc caggtctcgg cctgggccgg cgtccattac
      541 ttcgccagcc ggcacggctt tcacgcaccg ggcgaggagt ctgccgacgc aggcgacggc
      601 ctcctcacct ggcccgaagg caacgcatgg ctcgccgagc ggttggcggc acctctcggc
      661 gaacgcctgc gcgccggtcg ggtcgcgctg cgagtgcagg agggccgcga cgacgtgacc
      721 gtcgatgtct gggacgccgt cgccggtcgc cgcgagagat ggatcgcgac gcaggcgatc
      781 atcgcgacac cgctcttcat cgccgcgcgc ctcttcgatg cgccgccgcc ggcgctggcc
      841 gaagccgcgc cgctcgtgcg ccacgcgccc tggctggtcg ccaacctgca gctcgattcg
      901 gcgctcgacg actcgcccgg cgccccgctc tcgtgggaca acgtcatcta tcggggcgaa
      961 acgctcggct acgtcgacgc catgcaccag agtacgatgc ccttcgccgg cgccaccgtg
     1021 ctgagcgcct actgggccct cggcggagaa ggcgacgcca tgctgcgaac gcagcgccgt
     1081 cgcctgctcg acgacgactg gcgcgcctgg gcggcgcgca tcgtcgccga cctgtcggga
     1141 ccccatcccg acctggccgg ccggctgaag cgcgccgacc tcatgcgcta cggacacgcc
     1201 atggcgatcc cggcgcccgg cgtgcgcagc caccccgcct tgcgggcgct tgccgaaacc
     1261 acggggcggg tccatttcgc gcatgccgac ctgtcggcgt attcggtctt cgaggaagcc
     1321 ttcttccacg gaacccgcgc agggcgcgcc gctgccgcaa ccgcgatcgg ctgaacgcgg
     1381 ccgagcaacg ctgagcgctg cgccgccatc ttcaacctcg ccggcaatcg ggcgcgtgaa
     1441 ctgccgccca cactcgaccc aggcatggaa accgcccccg ggcttccgcg agcgccgcat
     1501 cggcgaacgc tggtggaaat gcgaaccgca gccggcgcgg acaccctgcg ccgcctcgct
     1561 tacagtcgct cgacccgagt tgcggagatg gcgatgatcc tgcttcgact gctgatcgca
     1621 acgatcgcgc tggcggtcgc cggcgtggcg aatgccgctc ccgcgtcgcc cgaatcggtc
     1681 gcgcggctgc tcgaggtgac acgggttcgc cagcaactcg aggtgatgag aacgcagtcg
     1741 ctgccgcgga tgctcgacgc cgtgcgatag tcgctcgccg ggcgacccga cggcgaggtc
     1801 gtcgagaagg cattcgatcg catgcagccg cgcttcctgg cgatcatgaa cgaggcgctc
     1861 gactgggaca cgatgcgccc gcagtacgtc gcgatgtacg tcgacacgtt ttcgcaggag
     1921 gaagtcgacg gtctgatcgc cttctaccag agcccgctcg gcacggcact cctcgacaag
     1981 atgccgctgc tgatccagcg ctcgatgcag ctcgcgcaac agcgaatgct gcccttgctg
     2041 caaaagatcg aggaaatggc gaaggaagaa gccacgacgt ccgggggcaa gccctgaacg
     2101 cgccacggcc ctcgatgcgc actccccgtt tcaacgtcgc catcgagcgc aatgccttcc
     2161 tggtcctgct tgtccttctg acgatcggct tcgcgtggat cgtctggccg ttctacggcg
     2221 cggtcttctg ggcggtcgtg ctcgccgtcc ttttcgagcc cttgaaccga cacgtccttc
     2281 gccgattcaa gggccgctcg acgctcgcgg cgctggcgac actcgccgtg atcctggtca
     2341 tcgtcatcct gccgacggca ttcgtcgcca tgtctttggt gcaggagatc gcgagcctgt
     2401 accagcgcgt tcgctcgggc gacgccaacc tggcgaccta cttcgatcaa ttctcggccg
     2461 cgttgccgtc gtgggcctcg cacctcatgg acaagctcgg cttcggcgac ctcgccggcg
     2521 tgcagggcac gctcacggcc gcgatcacgc agcgcggcga ggcgctcgcc gcgcgcgccg
     2581 tcaacgtcgg acaggacacc gtcgatttca tgctcggatt cggcctgtcg atgtacatcc
     2641 tcttcttcct gctgcgcgac gggccggcgc tcgccgccaa ggtccgaggt gcgctgcctc
     2701 ttgcgcccga gcccaaggcg cggctgctcg agcgcttcac caccgtcatc cgggccaccg
     2761 tcaagggcaa catcctcgtc gccgccgccc agggcgcgct cggaggtctg gccttctggg
     2821 tgctcga
//
LOCUS       DASRWS010000700         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3149206, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000700 DASRWS010000000
VERSION     DASRWS010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149206"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..672)
                     /locus_tag="VFF72_13390"
     CDS             complement(193..672)
                     /locus_tag="VFF72_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94208.1"
                     /translation="MNFEFARFHRCRPARPLLAFAVLALTGTLAVAAPASARHAASCV
                     AALKARETGLAVSINAGEPLQDEMLKVVRSGIAIIGTQYLAGLRESEARQMLDAAEKE
                     FDALPAGEAEARQASCLQEGDGLYRHASPIERSLIGTAAKRRMDRVVRSVAAAQDHG"
     gene            complement(914..2638)
                     /gene="soxB"
                     /locus_tag="VFF72_13395"
     CDS             complement(914..2638)
                     /gene="soxB"
                     /locus_tag="VFF72_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HZV94209.1"
                     /translation="MSPARREFLQLLAAGAAAGMTIDAGPVLAAQPGAAERLYDVPPF
                     GNVGLLHFTDCHAQLRPTYFREPSVNIGLGEASGRPPHLVGEALLKAFGIPAGSAQAH
                     AFTYLDFEAAARTYGRVGGFAHIATLVARLRASRPNALLLDGGDTWQGSATALWTNGQ
                     DMVDACKLLGVDAMTAHWEFTLGAKRVQEIVTKDLAGHIAFLAQNINTADFGDPVFDP
                     YILRPVNGVPVAVIGQAFPYTPIANPRYFVADWTFGIQDQNLQKIVDEARGKGAKVVV
                     VLSHNGMDVDLKMATRLRGVDAILGGHTHDGVPAPVVVKNAGGQTIVTNAGSNGKFLG
                     VLDLDVRDGRLRDFRYRLLPVFANMLPADPAMQAHIDRVRAPFLGKLEEPLAVNESLL
                     YRRGNFNGTWDQLLVDAIMAVRGAEIAFSPGFRWGTSLLPGQSITRELMMDQLAITYP
                     HATLTDMSGATIKTVLEDVCDNLFNPDPYYQQGGDMVRVGGLEYTCTPGAAMGARISD
                     MRLDGRPIDAGRTYKVGGWASVAEGAEGPPIWDVVETYLRDAKTIRPRRLNEPTLVGV
                     SANAGIGD"
     gene            complement(2667..>2773)
                     /locus_tag="VFF72_13400"
     CDS             complement(2667..>2773)
                     /locus_tag="VFF72_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HZV94210.1"
                     /translation="CSIMPRFGAAHILTTKQMQDIMALLLDPNSPVNQ"
BASE COUNT          419 a          945 c          980 g          429 t
ORIGIN      
        1 ctcaccggct acgggcggcc ggaagatcgc cgccgggccc tggacgccgg cttcgacgag
       61 cacatggtca agccggtcga tttcgacgcg ctgctgcgca tcatcgattc gatcgtcgcc
      121 gccgcgctcg ccgaaggcga cccgatgcgg gcagagtgaa cgcagccgtg ccgaggcgac
      181 cgcgccgtgt cgctaaccgt gatcctgcgc tgcggcgacg ctcctgacga cgcgatccat
      241 ccgccgcttc gccgcggtgc cgatcagcga gcgctcgatc ggcgacgcgt gccggtagag
      301 gccgtcgcct tcctgcaggc atgacgcctg gcgcgcctcg gcctcccctg ccggcaaggc
      361 gtcgaactcc ttctccgcgg cgtcgagcat ctgccgggcc tcgctctcgc gaagcccggc
      421 gaggtattgc gtgccgatga tcgcgatgcc gcttcgcacg accttgagca tctcgtcctg
      481 gaggggctcg ccggcgttga tcgataccgc cagccccgtt tcgcgggcct tgagggcggc
      541 gacgcacgac gcggcgtggc gcgcggaggc cggcgcagcc actgcgagcg tgcccgtcag
      601 cgccagcacc gcgaatgcca gcaggggccg cgccggccgg cagcgatgga atcgtgcaaa
      661 ttcgaaattc attggacgag atgaccttcg cgtcgctgtt caacggcatg acggcgcgac
      721 ggttgactcg cgccggagct catcctgcct cgatccgtcg acaggcgctg ccggtcgcgg
      781 cgacatccct atggccgatg ggcgaaaggt cgaggggccg agacgcgagc ccgggaaagg
      841 cgcgcaaacg ggcagaccgc gggtgccggc accgctcgct gccgcggccc cggcgggacg
      901 cggggcgtgc ggatcagtcg ccgatgcccg cgttggcgga cacgcccacg agcgtcggct
      961 cgttgaggcg gcgcggccgg atcgtcttcg cgtcgcggag gtacgtttcg acgacgtccc
     1021 agatcggcgg cccctcggcg ccctcggcga ccgacgccca gccacccacc ttgtaggtcc
     1081 tgccggcgtc gatcggccgg ccgtccaggc gcatgtcgga gatgcgcgcg cccatcgccg
     1141 cgcccggcgt gcaggtgtac tcgaggccac cgacacgcac catgtcgccg ccttgctgat
     1201 agtacggatc ggggttgaag aggttgtcgc agacgtcctc gagcacggtc ttgatggtcg
     1261 ccccgctcat gtcggtgagc gttgcgtgag ggtaggtgat ggcaagctgg tccatcatca
     1321 gctcgcgcgt gatcgactgc cccggcagca ggctcgtgcc ccagcgaaat ccgggcgaaa
     1381 aggcgatctc ggcaccgcgc accgccatga tcgcgtcgac cagcagctgg tcccaggtgc
     1441 cgttgaagtt gccacgccga tagagcaggc tctcgttgac ggcgagcggc tcctcgagct
     1501 tgccgaggaa cggcgcgcgg acgcgatcga tgtgcgcctg catcgccggg tcggccggca
     1561 gcatgttggc gaagacgggc agcaggcgat agcggaagtc gcgcagccgc ccgtcgcgca
     1621 cgtcgaggtc gagcacgccc aggaacttgc cgttcgagcc ggcgttggtg acgatggtct
     1681 ggccgccggc gttcttcacg acgaccggcg ccggcacgcc atcgtgggta tgaccgccga
     1741 ggatcgcatc gacgccgcgc agccgcgtcg ccatcttgag gtcgacgtcc atgccgttgt
     1801 gcgagaggac gacgacgacc ttcgcgccct tgccgcgcgc ttcgtcgacg atcttctgca
     1861 ggttctggtc ctggatgccg aaggtccagt cggcgacgaa atagcgcgga ttggcgatcg
     1921 gcgtgtacgg aaacgcctgg ccgatcaccg ccaccgggac gccgttgacc gggcgcagga
     1981 tgtacggatc gaagaccggg tcgccgaaat cggcagtgtt gatgttttgc gccaggaagg
     2041 cgatgtggcc ggcgagatcc ttcgtgacga tctcctgcac gcgcttggcg cccagcgtga
     2101 actcccagtg cgctgtcatc gcgtcgacgc cgagcagctt gcaggcgtcg accatgtcct
     2161 ggccattcgt ccagagtgcc gtcgccgaac cttgccacgt atcgccgccg tcgaggagca
     2221 gcgcgttcgg ccggctggct cgcaaccgcg cgaccagcgt ggcgatgtgc gcgaagccgc
     2281 cgacgcggcc gtaggttcgc gccgccgctt cgaagtcgag gtaggtgaag gcgtgcgcct
     2341 gcgcgcttcc ggcggggatg ccgaaggcct tcagcagcgc ctcgccgacc agatgcggcg
     2401 gccggccgct cgcctcgccc aggccgatgt tgacgctcgg ctcgcgaaag taggtcggcc
     2461 gcaattgcgc atggcagtcg gtgaaatgga gcaggccgac atttccgaac ggcggcacgt
     2521 cgtaaagacg ctccgcggcg ccgggctgcg cggcgagcac cggcccggca tcgatcgtca
     2581 tgccggcagc agcccctgcg gcgagcaact gcaggaactc gcgccgggcc gggctcatcg
     2641 ccgagtctcc ggcgccccgc cgcgcatcac tggttgaccg gcgaattcgg atcgagcagc
     2701 agggccatga tgtcctgcat ctgcttggtc gtcaggatgt gcgcagctcc gaagcgcggc
     2761 atgatcgagc agg
//
LOCUS       DASRWS010000701         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1994862, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000701 DASRWS010000000
VERSION     DASRWS010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994862"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFF72_13405"
     CDS             complement(<1..1148)
                     /locus_tag="VFF72_13405"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZV94211.1"
                     /translation="MSEHRIEKDTFGEIAVPAARLWGAQTERSLHHFDISTEKMPPEL
                     IRALALVKRSAAVVNRGIGALDPKKADAIIAAADEVLEGRHDNEFPLAVWQTGSGTQS
                     NMNMNEVLANRASQLLGGPLGEGRLVHPNDDVNRSQSSNDVFPTAMSVAAVEAVMRRL
                     IPPLEALRTTLQDKADAFADIVKIGRTHLQDATPLTLGQEISGWVAQLEHGLVHVVAA
                     LPHLCELALGGTAVGTGLNSPPGFAEKVAEELAERTGLPFVSAPNKFEALAAHDAIVH
                     GHGALKTLAASLTKIANDVRWLASGPRSGLGEITIPENEPGSSIMPGKVNPTQCEALT
                     MLSAQVLGNDVAINVAGASGNFELNVFKPVLIHNFLQSARLLGDGARS"
     gene            1201..2253
                     /locus_tag="VFF72_13410"
     CDS             1201..2253
                     /locus_tag="VFF72_13410"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZV94212.1"
                     /translation="MAFVPYPLARPFLFALDAEHAHELTLRGLAAIQRTPLECAVMAG
                     RVDDPVTIAGLHFANRIGLAAGLDKNGRCIDAFGAMGFGSIEVGTVTPLAQPGNPRPR
                     LFRLPEANALINRLGFNNDGLAAFIANVRQARFRERGGILGLNIGKNAATPIERAADD
                     YLACLSGVYPHADYVTVNISSPNTKSLRDLQGEARLDALLEAIGRRRDELADEHARIV
                     PVFLKIAPDLDGVEVERVAATLRRHRIDGVVATNTTLGRAAVARLPHGQESGGLSGAP
                     LLAASNAVIAQLRGALGANYPIIGVGGVLDAADAVAKRNSGADVVQIYTGLIYRGPAL
                     VGAAATALRDTPATRR"
     gene            2448..3338
                     /locus_tag="VFF72_13415"
     CDS             2448..3338
                     /locus_tag="VFF72_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94213.1"
                     /translation="MTRTIPASLAHVVRAAVLAVVAVLLIPAAFAQDASALRARQAAL
                     HDRFSSNPFGRPLVLESTQTSSGLRGDVYAIVDHPFSAVQPALQSIDHWCDILILHLN
                     VKHCRANSANKTINLRVGRKFDQPIEDAYELDFKFAVAAATTDYLQILLTAKDGPLST
                     KDYRIQVEAIPSGDGKSLIHMSYAYGYGFAARLAMETYLATIGRDKVGFSVVDHKNGK
                     PVYVGGVLGLLERNTMRYYLAIDAFLDAASLPAAQQPEQRIRDWYASTERYARQLHEM
                     DENDYLDMKRKEIRLDRKPG"
     gene            complement(3381..4019)
                     /locus_tag="VFF72_13420"
     CDS             complement(3381..4019)
                     /locus_tag="VFF72_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZV94214.1"
                     /translation="MPWTHDQMAARAAEELQDGFYVNLGIGLPTLVANFVKPDIEVWL
                     QSENGMLGIGPFPTEDEVDADLINAGKQTVTTIPGSSIFGSHDSFAMIRGGKINLSIL
                     GAMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVERVLVLMEHVARKKDGGFDLKILP
                     TCTLPLTGVGVVDRIITDLAVMDVTPQGLHVVAIADGVTREELQARTGVALR"
     gene            complement(4098..>4379)
                     /locus_tag="VFF72_13425"
     CDS             complement(4098..>4379)
                     /locus_tag="VFF72_13425"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZV94215.1"
                     /translation="SGNLVFRRTARNFNPAVAMAGRITIVEVEEIVETGAIDPDAVHL
                     PGIYVHRIVLNAAPEKRIEKRTVRAAVPADVAVQEAIEVIAKAAEKAGV"
BASE COUNT          721 a         1517 c         1405 g          736 t
ORIGIN      
        1 aagctcctcg cgccgtcgcc gaggagccgc gcgctctgca ggaagttgtg gatcagcacc
       61 ggcttgaaga cgttgagctc gaaattgccc gacgcgccgg cgacgttgat cgcgacgtcg
      121 ttgccgagca cctgcgcgga cagcatggtc agcgcctcgc actgcgtcgg attgaccttg
      181 cccggcatga tcgagctgcc cggctcgttc tcggggatcg tgatctcgcc gagcccggag
      241 cgcggcccgg acgcgagcca gcggacgtcg ttggcgatct tggtgagaga cgccgcgagc
      301 gtcttcagcg caccgtgccc gtggacgatc gcatcgtgcg cggcgagcgc ctcgaacttg
      361 ttcggcgccg acacgaatgg caagccggtg cgttcggcga gctcctcggc gaccttctcg
      421 gcgaaaccgg gcggcgagtt gaggccggtt ccgaccgcgg tgcctcccag cgccaattcg
      481 cacagatgcg gcagcgcggc aacgacatgc acgaggccat gctcgagctg ggcgacccat
      541 cccgaaatct cctggccgag ggtcaggggc gtggcgtcct gcagatgcgt ccggccgatc
      601 ttgacgatgt cggcgaaagc gtcggccttg tcctgcaagg tcgttcgcaa agcttccagg
      661 ggcggaatca gcctgcgcat caccgcttcg acggcggcga cactcatcgc ggtcggaaaa
      721 acgtcgttcg acgactggct tcgattcaca tcgtcgttcg gatgaacgag cctgccttcg
      781 ccgagcgggc cgccgagcag ctgcgaggcc cggttggcga gcacctcgtt catgttcatg
      841 ttcgattgcg tgcccgaacc ggtttgccag acggcgagcg gaaactcgtt gtcgtgacga
      901 ccttccagga cctcgtcggc ggcagcgatg atggcgtctg ccttcttcgg gtccagcgca
      961 ccgatcccgc gattcacgac cgcggcgctg cgcttgacga gcgcgagcgc ccggatcagc
     1021 tccgggggca tcttctcggt ggagatgtcg aagtgatgca acgagcgttc ggtctgcgcc
     1081 ccccacaggc gcgccgccgg cacggcgatc tcgccgaagg tgtctttctc gatccggtgt
     1141 tcgctcatcg cattggcccc ggcacgagta tgcaacggca atgccagaat cgcgcgtccc
     1201 atggccttcg tcccgtatcc gctcgcccgc cctttcctct tcgcgctcga tgccgagcat
     1261 gctcacgagc tgaccctgcg cgggctcgcc gccatccaac gcaccccgct cgaatgcgcg
     1321 gtgatggccg gtcgcgtcga cgacccggtg acgatcgccg gtttgcattt cgcgaaccgg
     1381 atcgggctgg ccgccgggct cgacaagaac ggacgctgca tcgacgcctt cggggcgatg
     1441 ggattcggca gcatcgaggt cggcaccgtg acgcccctcg cgcagcccgg caacccgcgg
     1501 ccgcgactgt ttcgcctgcc cgaagcgaac gccctgatca accgcctcgg attcaacaac
     1561 gacggcctgg cggcattcat cgccaacgtc cggcaggcgc gctttcgcga gcgcggcggc
     1621 atcctcggcc tcaacatcgg caagaacgcg gcgacaccga tcgagcgcgc cgccgacgac
     1681 tatctcgcgt gcctgtcggg cgtctacccg cacgcggact acgtgacggt caacatctcg
     1741 agtccgaaca cgaagagcct gcgagatctg cagggcgaag cccggctcga tgcgctcctc
     1801 gaggccatcg gccggcggcg cgacgagctg gccgacgagc acgcgcgcat cgtccccgtc
     1861 ttcctgaaga tcgcccccga cctcgacggg gtcgaggtcg aacgggtcgc ggcgacgctg
     1921 cggcggcacc ggatcgacgg cgtcgtcgcc accaacacga cgctcggccg cgcggcagtg
     1981 gcccgattgc cgcatggaca ggaaagcgga ggactgtcgg gagcgccgct gcttgccgcg
     2041 tcgaacgccg tcatcgcgca gctgcgaggc gcgctcggcg cgaactaccc gatcatcggc
     2101 gtcggtggcg tcctcgacgc cgccgatgcg gtggccaagc gcaattcggg tgccgatgtc
     2161 gtgcagatct acaccgggtt gatctatcgg gggccggcgt tggtcggcgc ggccgccacg
     2221 gcactgcgcg acacaccggc gacaaggcgt tgacagcgac ccggcgccgc ttcctacaaa
     2281 tcgaactgcg ctcgcgccgc tatgctctgc agcatcctga cgactcgttc atcggccatc
     2341 cgacgttgcc caccccggac gcggaacagg gccgggaacg atcgcccgcc agatcgcccg
     2401 cgaaacttcg cccgcatgcc caatgtgaaa tccagggagc ttcggacttg acgcgcacca
     2461 tccctgcttc cctcgctcac gtcgttcgcg cggcggtgct ggccgtcgtg gcggtgctgc
     2521 tgattcccgc cgcgttcgca caagacgcat cggccttgcg cgcacgccag gcggcacttc
     2581 atgacaggtt cagcagcaat ccgttcggac ggccgctggt tctcgagtcg acgcagacgt
     2641 caagcggcct gcgcggcgac gtctacgcca tcgtcgatca cccgttcagt gccgtccagc
     2701 cggcgctgca atcgatcgat cactggtgcg acatcctgat cctgcatctc aacgtgaagc
     2761 attgccgggc gaactctgcg aacaagacga tcaacctgcg cgtcggccgc aagttcgacc
     2821 agccgatcga ggacgcctac gagctcgatt tcaagttcgc cgtcgccgct gcgacgaccg
     2881 actatctcca gatcctcctg accgccaagg atgggccgct cagcaccaag gactaccgca
     2941 tccaggtgga ggcgatccct tccggcgacg gcaagagcct cattcacatg tcctacgcct
     3001 acggctacgg cttcgctgcc cgactggcga tggagaccta tcttgcgacc atcggccgcg
     3061 acaaggtcgg cttttcggtc gtcgaccaca agaacggaaa accggtctac gtcggcggcg
     3121 tgctcgggct gctggagcgc aacacgatgc gctactacct ggcgatcgac gccttcctcg
     3181 acgccgcctc gctgcccgca gcgcagcagc cggagcagcg gatacgcgac tggtacgcga
     3241 gcaccgagcg ctacgccagg cagctccacg agatggacga gaacgactat ctcgacatga
     3301 agcgcaagga gatccgcctc gatcggaaac cgggctgagc gcagcgcccg ctgcgcaatg
     3361 ccgccgccgt gcccgaaggc tcagcgcagc gcgaccccgg tcctggcctg cagttcttcg
     3421 cgggtgacgc cgtcggcgat cgccacgacg tgcagcccct gcggggtgac gtccatgacc
     3481 gccagatcag tgatgatccg gtcgaccacg ccgacgccgg tgagcggcag ggtgcaggtc
     3541 ggcaggatct tcaggtcgaa cccgccgtcc ttctttcgcg cgacgtgctc catgagcacg
     3601 agaacgcgct cgacgccggc gacgaggtcc atcgcgccgc ccatgccctt gaccatcttg
     3661 cccgggatca tccagttggc aagatcgccc ttctcgctca cctgcatggc gccgaggatc
     3721 gacaaattga tcttgccgcc gcgaatcatc gcgaacgaat cgtgcgagcc gaagatcgac
     3781 gagccgggga tcgtcgtcac ggtctgcttg ccggcgttga tgagatcggc gtcgacctcg
     3841 tcttcggtcg ggaacgggcc gatcccgagc atgccgttct cgctctgcag ccagacttcg
     3901 atgtcgggct tgacgaaatt cgcgacgagc gtcggcagtc cgatcccgag gttgacgtag
     3961 aagccgtcct gcagctcctc ggcggcacgc gccgccatct gatcgtgggt ccagggcatg
     4021 tcagcttcct tggtgccgca tcgagacggc gtggcccgtg aacgggcggt aaatggcgtc
     4081 ggcgcgggtg ttcatcctca gactcccgcc ttctcggcgg ccttggcgat cacctcgatc
     4141 gcttcctgga ccgcgacgtc ggcaggaacg gcagcccgca cggtgcgctt ctcgatccgt
     4201 ttctcgggcg cggcattgag aacgatgcgg tgcacgtaga tgcccggcag atgcaccgcg
     4261 tcgggatcga tcgctccggt ctcgacgatc tcctcgactt cgacgatcgt gatcctgcct
     4321 gccatcgcca cggccgggtt gaaattgcgc gccgtccgcc tgaacacgag gttgccgct
//
LOCUS       DASRWS010000702         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3149868, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000702 DASRWS010000000
VERSION     DASRWS010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149868"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFF72_13430"
     CDS             complement(<1..582)
                     /locus_tag="VFF72_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94216.1"
                     /translation="MSLPMLTVPALAARPAPAADDPPPARKLRILILGGTGFTGPFQV
                     RYALARGHAVTLFNRGKSPHRWSGAVEELVGDRNTGDLRALRGRDWDVCIDNPTMLPA
                     WVRDAGQALHGHVGRYVFVSTVSVYATDDRPDADESAALAAYTGKDAMAETMQSVRDS
                     GFALYGPLKAHSEQEAARRFPGIVTIIRPGLIAG"
     gene            complement(649..1629)
                     /locus_tag="VFF72_13435"
     CDS             complement(649..1629)
                     /locus_tag="VFF72_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZV94217.1"
                     /translation="MATMSRRGFSGLAGATLLGGAAPGPVAAFCAGASSAVALATASA
                     AANAAAGNAAPDAAVLMRAIPGTGERLPAVGLGTAGVFDRDDASTRDAASAVLRALLE
                     HGGKLVDTASSYGDAEQVLGTAMSAAGWRDRLFVATKVEALDDDELARSLARLKTARV
                     DLLQLHNVRDADRSLARLREWKAKGRCRYVGITSTYHRDFAAVESVLRREKPDVVQID
                     YSLDDRIAEERILPAAADVHAAVLTALPFGRGRLFRAVRGKAIPEWAREFAGSWSHFF
                     LKYLLGDERVTAVIPGTSNPAHMADNAGAMRGRLPDAGERRRMVSFIDAL"
     gene            complement(1632..2927)
                     /locus_tag="VFF72_13440"
     CDS             complement(1632..2927)
                     /locus_tag="VFF72_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZV94218.1"
                     /translation="MTDVAGWLRRTMPATPAERSAALWSFGYFFMLLASYYVLRPLRD
                     RMGIAGGVRALPWLFTATFVTLLVAQPIYGALVARLPRGRFVPIVYQFFAANLALFWL
                     LLTLAVEPVIVARVFFVWVSVFNLFAVAVFWSFMADLFTSEQGKRLFGFIGAGGTAGG
                     LLGPAITILLAVPLGPANLLLVAIVFLELAVFCVARLERSSGASAGAGPVRRDDRRIG
                     GSALAALPELVRSPYLLGLGAWVALLSLCATVLYLTQAHVVAAAVHGGGAQTRIFAGI
                     DLAVGLLTLATQVFATGRLIERFGTGIAAGTLPAVYLAGFAALALSPVLAVVLVVQVV
                     QRWANFAVANPARQVFFTVLDREEKYKAKNLIDVVIYRGSDAASSWLFESLQAIGLKL
                     GAVALCALPVAAAWLWLSTTLGRMHERRAARPGAASSGD"
     gene            complement(2924..>3244)
                     /locus_tag="VFF72_13445"
     CDS             complement(2924..>3244)
                     /locus_tag="VFF72_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HZV94219.1"
                     /translation="DEVERDGIGVTTQKYLPGARVVRAFNTLGYTLLAREADRAPPRL
                     AIPIAGDDAAAVQVAAGLVRDAGFDPVVVGSLVDARRFQRGGPGYGQAVSVDQLRRVL
                     SLPP"
BASE COUNT          538 a         1148 c         1174 g          384 t
ORIGIN      
        1 gccggcgatc aggccgggcc ggatgatggt gacgatgccc gggaaccggc gcgcggcctc
       61 ttgttcggaa tgcgccttga gcggcccgta gagcgcgaag ccgctgtcgc gcaccgactg
      121 catcgtctcg gccatcgcgt ccttgccggt gtaggcggcg agcgctgccg attcgtcggc
      181 gtcgggccga tcatcggtcg cgtagacgga aaccgtcgag acgaacacat agcgaccgac
      241 gtggccatgc aacgcctgcc cggcatcgcg cacccaggcc ggaagcatcg tcgggttgtc
      301 gatgcagacg tcccagtcgc ggccccgcag cgcccgcagg tcgccggtgt tgcgatcgcc
      361 gaccagctcc tcgactgcgc ccgaccagcg atgcggcgat ttgccgcggt tgaagagggt
      421 gacggcgtgg cctcgcgcca gcgcgtagcg cacctgaaag gggccggtga agccggtacc
      481 cccgaggatc aggatgcgca gcttgcgcgc tggcggcggg tcgtcggccg ctggcgccgg
      541 acgagccgcg agggccggca cggtgagcat cggcaggctc acgaaactcc tgcgacccgg
      601 ctgcatggga gtcctccgac ccggcgcccg cgcgccttcg cgagacgctc agagcgcatc
      661 gatgaacgac accatgcgcc ggcgctcgcc ggcgtcggga agccttcccc gcatggcgcc
      721 ggcgttgtcg gccatgtgcg ccgggttcga cgtgcccggg atcaccgccg tgacccgctc
      781 gtcgccgagc aggtatttga ggaagaaatg cgaccagctg ccggcgaact cgcgcgccca
      841 ttcggggatg gccttgccac gcaccgcgcg aaacagccgg ccgcgaccga acgggagggc
      901 cgtgaggacg gcggcgtgca cgtcggctgc cgccggcagg atgcgctcct cggcgatgcg
      961 gtcgtcgagc gaataatcga tctgcacgac gtcgggcttc tcgcgtcgca gcaccgattc
     1021 gacggctgcg aaatcacggt ggtacgtcga cgtgatgccg acatatcggc agcggccctt
     1081 cgccttccac tcccgaagcc gtgccagcga gcgatcggcg tcgcggacgt tgtgaagctg
     1141 cagcaggtcg acccgcgccg tcttcagccg ggccagcgaa cgggcgagct cgtcgtcgtc
     1201 gagtgcctcg accttggtcg ccacgaacag ccggtcgcgc cagccggcgg cgctcatcgc
     1261 cgtgcccagc acctgctcgg cgtcgccgta cgacgacgcc gtgtcgacga gcttgccgcc
     1321 gtgctcgagc agcgcgcgaa gcaccgccga cgcggcgtcg cgcgtcgacg cgtcgtcgcg
     1381 gtcgaagacg ccggccgtgc ccaggccgac ggcgggcagg cgctcgccgg ttccgggaat
     1441 cgcgcgcatc agcaccgccg catccggcgc ggcgttgccg gccgccgcgt tggccgcggc
     1501 actggcggtc gccagcgcca cggctgaaga agcgcctgcg cagaaggccg ccaccggacc
     1561 cggcgccgcg ccgcccagca gtgtcgcgcc cgccagaccg gaaaagccgc gtcgagacat
     1621 cgttgccatc gtcaatctcc tgaagacgcc gcgcccggtc gagccgcgcg tcgttcgtgc
     1681 atgcggccca gcgtcgtcga gagccacagc cacgccgcgg cgacgggcag cgcgcagagc
     1741 gcgaccgcgc cgagcttgag gccgatcgcc tgcaggctct cgaacagcca gctcgacgcg
     1801 gcatccgagc cgcgatagat gaccacgtcg atcaggttct tcgccttgta cttctcttcg
     1861 cgatcgagga cggtgaagaa gacctgccgc gccggattgg cgacggcgaa gttggcccag
     1921 cgctgcacca cctggacgac caggacgacc gcgagcaccg gtgaaagcgc cagcgccgcg
     1981 aagccggcca ggtacaccgc cggcagcgtg ccggcggcga tgccggtacc gaagcgctcg
     2041 atcagccggc cggtcgcgaa cacctgcgtc gccagggtca gcaagccgac ggcgaggtcg
     2101 atgccggcga agatccgggt ctgcgcgccg ccgccgtgca ccgccgcggc gacgacatgc
     2161 gcctgcgtga gatagagcac cgtcgcgcac aacgacagca gcgccaccca ggcgccgagg
     2221 ccgagcaggt acggcgagcg gacgagttcg ggcagcgcgg cgagggcgct gccgccgatg
     2281 cggcgatcgt cgcggcgcac cgggccggcg cccgcagaag ctccgctgct gcgctcgagg
     2341 cgggccacgc agaacaccgc gagctcgaga aagacgatgg cgacgagcag caggttggcc
     2401 ggaccgagag gcaccgcgag caggatcgtg atcgccggcc cgagcaatcc gccggccgtg
     2461 cccccggcgc cgatgaaccc gaacagccgc ttgccctgct cgctcgtgaa gaggtcggcc
     2521 atgaacgacc agaacacggc gacggcgaag agattgaaga cgctgaccca gacgaagaag
     2581 acgcgcgcca cgatcaccgg ctcgacggcc agggtgagga gcagccagaa cagcgccagg
     2641 ttggcggcga agaactggta gacgatgggc acgaagcggc cgcgcggcag cctcgccacc
     2701 agcgcgccgt agattggctg ggcgacgagc agcgtcacga acgtcgcggt gaagagccac
     2761 ggcagcgcgc gcacgccgcc ggcgatgccc atgcgatcgc gcaaaggccg cagcacgtag
     2821 tagcttgcca gcagcatgaa gaagtagccg aacgaccaga gcgccgcgga gcgctcggcg
     2881 ggcgtggccg gcatggtgcg gcgcagccaa ccggcaacgt cggtcatggc ggcagcgaca
     2941 gcactcttcg cagctggtcg acgctcaccg cctggccgta accggggccg ccgcgctgga
     3001 agcggcgggc gtcgacgagc gagccgacga cgaccggatc gaagccggcg tcgcgaacga
     3061 gccccgccgc gacctgcacc gccgcggcat cgtcgccggc gatcgggatg gccaggcgcg
     3121 gcggcgcgcg gtccgcctcg cgcgcaagaa gggtgtagcc gagcgtgttg aaggcgcgga
     3181 cgacgcgcgc gcccggcagg tacttctgcg tcgtgacgcc gatgccgtcg cgctcgacct
     3241 cgtc
//
LOCUS       DASRWS010000703         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_1996802, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000703 DASRWS010000000
VERSION     DASRWS010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996802"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFF72_13450"
     CDS             <1..942
                     /locus_tag="VFF72_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZV94220.1"
                     /translation="ALPDDPKEAIRAARAIGYPVIIKAAGGGGGRGMRVVHTEAALVS
                     AVQTTRAEAGAAFGNPAVYMEKFLENPRHIEIQILADQHKNVIWLGERDCSMQRRHQK
                     IIEEAPAPGIPRRAVEKIGERCVAACKRIGYRGAGTFEFLYENGEFFFIEMNTRVQVE
                     HPVTEWVTGIDIVQMQLRIAAGEKLTLAQRNVQCRGHAIECRINAEDPYKFTPSPGRV
                     TMWHPPGGPGVRVDSHAYTNYVVPPNYDSMIGKIIVHGDTREQALARMRTALSETVVE
                     GIQTNIPLHRELMVDAKFVEGGTSIHYLEGWLDQHKR"
     gene            953..1870
                     /gene="prmA"
                     /locus_tag="VFF72_13455"
     CDS             953..1870
                     /gene="prmA"
                     /locus_tag="VFF72_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZV94221.1"
                     /translation="MHALTLLADAGSVDALSERLAVELDALSVSVEDADAGTPAEQPV
                     FDEPGTAAAPGWRHARVTALFVDETAATAAAAAISASAIGSTDRVESIDAVDDRDWVR
                     LTQSQFGPIEIASGFWIVPTWSPVPPTARQVIRLDPGMAFGTGTHPTTRMCLRWIVQA
                     AARARHPWRSVLDYGCGSGVLAIAAALLGAESVEAVDIDPAAVEATAQNARFNGVSIR
                     AGQADRLAVEPRSLVVANILATPLKLLAPVLAAQLAPAGELVLSGILERQAGELRAAY
                     ASWLDLDVTASDDGWILMTGTRTGECRVA"
     gene            1878..3149
                     /locus_tag="VFF72_13460"
     CDS             1878..3149
                     /locus_tag="VFF72_13460"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HZV94222.1"
                     /translation="MSLATRCSACATVFRVAQDQLKVSEGWVRCGRCDAVFNALEGLF
                     DLGREQPGDIDTPIRTPSPAPAVAAGATAASPAPSQATSAEPSSALPSSPHADAPRGG
                     ASTAAASHSATPHMFAPPVSAMPAPAAPGSRPSAPAAAPELSIASSGHDDGNDDKDDY
                     PPPLAGHAGSVYGIPQSPTDGLLADPIDAHLFRKRGADPDKSPAVEVDARDRLEFSDA
                     RFDSDLFAENPSSLETTLVEVGTTDAGDLPLESASQQPDFLRRADRRGYWRSGPRRLA
                     LAIISIAAAVLLGMQVAGHFRDIVAAQWPALRPLLVAGCRVAGCSVEAPRRIDEVSVE
                     SSSLSRAIGQDAFVLSVTLRSRSRIALRTPSIDLTLTDGNGRLVARRALSPKDFGVAG
                     VLPAGAESALQFMLATGNYRVAGYTVEIFYP"
     gene            3185..>3570
                     /locus_tag="VFF72_13465"
     CDS             3185..>3570
                     /locus_tag="VFF72_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZV94223.1"
                     /translation="MAALICGSLAFDSIATFPGRFSEQILPDQLHILNVSFLVPELRR
                     EFGGCAGNIAYNLAALGGEAVVVAAIGKDGGGYVERLRSWGVDTAQVREIDASLTAQA
                     FIITDLDNNQITAFHPGAMQDAHRIA"
BASE COUNT          544 a         1257 c         1194 g          575 t
ORIGIN      
        1 gcgttgcccg acgacccgaa agaagcgatt cgcgccgccc gggcgatcgg ctaccccgtg
       61 atcatcaagg cggcgggagg cggaggcggc cgcggcatgc gcgtcgtgca tacggaagcc
      121 gctctcgtct cggccgtgca gacgacgcgg gccgaggccg gcgcggcgtt cggaaatccg
      181 gccgtctaca tggagaagtt tctcgagaat ccccgccata tcgagatcca gatcctcgcc
      241 gaccagcaca agaacgtcat ctggctcggc gagcgcgatt gttcgatgca gcgtcggcac
      301 cagaagatca tcgaggaagc gccggcgccg ggcatccccc gtcgcgccgt cgaaaagatc
      361 ggcgagcgct gcgtcgcggc ctgcaagcgc atcggctacc ggggcgccgg cacgttcgag
      421 ttcctgtacg agaacggcga gttcttcttc atcgagatga acacgcgggt ccaggtggag
      481 catccggtga ccgagtgggt gaccggcatc gacatcgtgc agatgcagtt gcgcatcgcc
      541 gccggtgaaa agctcaccct ggcgcagcgc aacgtccagt gccgcggcca cgcgatcgaa
      601 tgccgcatca acgccgagga tccgtacaag ttcacgcctt cgccagggcg cgtgacgatg
      661 tggcatccgc ccggcggccc gggggtgcgc gtcgattcgc atgcctacac gaactacgtc
      721 gttccgccca actacgactc gatgatcggc aagatcatcg tccatggcga cacccgggag
      781 caggcgctgg cccgcatgcg cacggccctt tccgagaccg tggtggaggg catccagacc
      841 aatatcccgc tgcatcgcga gctgatggtc gatgcgaagt tcgtcgaggg cggaacgagc
      901 atccactacc tggaaggctg gctcgatcag cacaagcgct gagggcggcg tcatgcacgc
      961 gctgaccctg ctcgccgatg ccggctcggt cgatgcactg tcggagcgcc tcgccgtgga
     1021 gctcgacgcg ctctctgttt ccgtcgagga tgccgacgcc ggcacgccgg ctgagcagcc
     1081 cgtcttcgac gaaccgggga ccgcggctgc gcctggctgg cgacacgccc gcgtcacggc
     1141 cctcttcgtc gacgagacgg cggcgactgc cgcggcggcg gccatcagcg cgtctgccat
     1201 cggctccacg gatcgtgtcg agtcgatcga cgctgtcgat gaccgcgact gggttcgact
     1261 gacgcagtcg cagttcgggc cgatcgagat cgcgagcggg ttctggatcg tgccgacatg
     1321 gtcgccggta ccgccgacgg cccgccaggt gatccggctc gatcccggca tggccttcgg
     1381 caccggcacg cacccgacga cgcgcatgtg cctgcgctgg atcgtccagg cagccgcgcg
     1441 cgcgcggcat ccctggcgaa gcgtgctcga ttacggatgc ggctcggggg tgcttgcgat
     1501 cgctgcagca ctgctcggcg ccgaaagcgt cgaggcggtc gacatcgacc cggcggcggt
     1561 cgaggcgacg gcgcagaacg cgcgcttcaa tggtgtctcg attcgcgccg gccaggccga
     1621 tcgtctcgct gtcgaacctc gctcgctggt cgtcgccaac atcctggcca cgcccctcaa
     1681 gttgctggcg ccggttctgg ccgcgcagct ggcgccggcc ggcgagctcg tcctctcggg
     1741 catcctcgag cggcaggccg gcgagcttcg cgccgcgtac gcgagctggc tcgacctcga
     1801 cgtgacggcg agcgacgacg ggtggatcct gatgaccgga acgcggaccg gcgaatgccg
     1861 cgtggcatga tttcgcgatg agcctggcga cccgatgctc ggcatgcgcg acggtctttc
     1921 gcgtcgccca ggatcagctg aaggtttccg agggctgggt ccgttgcggc cgctgcgacg
     1981 ctgtcttcaa tgcactcgag gggcttttcg acctcggccg cgaacagccc ggcgacatcg
     2041 acacgccgat tcgcacgcca tcgccggcgc cggcggtcgc cgccggtgcc acggcggcat
     2101 cacccgcccc ttcgcaggca acttcggccg agccttcgtc ggcgctcccg tcctcgccgc
     2161 atgccgacgc gccgcgtggc ggggcttcga ctgccgccgc gtcgcattcc gccacgccgc
     2221 acatgttcgc gccgccggtc tcggcgatgc ccgcgccggc ggcacccgga tcgaggcctt
     2281 cggcgcctgc agccgcgccc gagctgtcga tcgcgtcgtc gggccacgac gacggcaacg
     2341 atgacaagga cgactacccg ccgccgctgg ccggccatgc cggctcggtc tacggcatcc
     2401 cgcaatcgcc gaccgacggc ctgctggccg atccgatcga tgcacacctg tttcgaaagc
     2461 gtggcgccga ccccgacaaa tcgccagccg tcgaggtcga tgcacgggat cgcctcgaat
     2521 tctcggacgc ccgcttcgac tccgaccttt tcgccgagaa cccgtcctcc ctggagacga
     2581 cactcgtcga ggtgggcacg acggatgcgg gcgacctgcc gctcgaaagc gccagccagc
     2641 aacccgactt cctgcgccgc gcggatcgcc ggggctactg gcgcagcggc ccgcgacgcc
     2701 tcgcgctggc catcatttcg atcgccgcgg ccgtgctgct cgggatgcag gtggccggac
     2761 attttcgcga catcgtcgca gctcaatggc ctgcgcttcg tccgctgctc gttgccggat
     2821 gccgggtggc cggttgcagc gtcgaggcgc cgcgacggat cgacgaggtc tccgtcgaaa
     2881 gcagttcgct gtcgcgggcg atcgggcaag atgccttcgt tctttcggtg acgctgcgca
     2941 gccgaagccg catcgcgttg aggacaccgt cgatcgatct gaccctgacg gacggcaacg
     3001 gccgcctggt ggcgcgccgg gcgctttcgc cgaaagactt cggtgtcgcg ggcgtccttc
     3061 cggccggtgc cgaatcggcc cttcagttca tgctcgccac cggcaactat cgcgtcgccg
     3121 ggtacaccgt cgagatcttc tatccctgaa attcgcggcc ccgcgccgcc acggagtcgt
     3181 tcaaatggcc gccctcatct gtggatcgct cgccttcgac agcatcgcga cctttcccgg
     3241 cagattctcg gagcagatcc tgcccgacca gctgcacatc ctcaacgtct cgttcctggt
     3301 tcccgagctg cgccgcgagt tcggtggctg cgccggcaac atcgcgtaca acctcgccgc
     3361 gctgggcggc gaggcggtcg tcgtggcggc gatcggcaag gacggcgggg gctacgtcga
     3421 gcggctgcgc tcgtggggcg tggacacggc ccaggtgcgc gagatcgacg cgtcgttgac
     3481 ggcgcaggcc ttcatcatca ccgacctgga caacaaccag atcaccgcat tccatccagg
     3541 cgcgatgcag gatgcccatc ggatcgccat
//
LOCUS       DASRWS010000704         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2578906, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000704 DASRWS010000000
VERSION     DASRWS010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578906"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1060)
                     /locus_tag="VFF72_13470"
     CDS             complement(101..1060)
                     /locus_tag="VFF72_13470"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZV94224.1"
                     /translation="MTRRAVVKGVGSALPRRKVTNDELAATVDTSDEWIVERTGIRSR
                     YIASEGETTGSLATEAARRALDDAGIDAGEISLIVLATATPDQTFPSTATKVQAALGI
                     NDCIAFDVHAVCTGFLYALSVADSMLRSGGADKALVIGAETFSRILDWEDRATCVLFG
                     DGAGALVLSAEDTESGILSTKLHADGRHNDLLFVDGGPSTTGTVGKLRMKGREVFRHA
                     VINLAGVMNEVLAGAGLVADDVDWVVPHQANARILDATAKKLGLPPQKVVVTVDRHAN
                     TSAASVPLAFDTAVKDGRIKRGDIVVLEAMGGGFTWGAAALRY"
     gene            complement(1057..2106)
                     /gene="plsX"
                     /locus_tag="VFF72_13475"
     CDS             complement(1057..2106)
                     /gene="plsX"
                     /locus_tag="VFF72_13475"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZV94225.1"
                     /translation="MSGGPRIAIDAMGGDAGPKAMIAGASHALRKDPTLEFTFYGDEV
                     RVAAEIGVHRNLAGRSQLVHSPEAIAPSEKPSQAIRRARTTSMGMAINAVKEGVADAA
                     LSGGNTGAMMAMSKLALRTMPGIDRPALAALLPTLGDNDVIMLDLGANTECDAQNLVQ
                     FAVMGAAYSRTVLGIAAPRVKLLNIGTEELKGTDELKDAAALLREADYLPFRFDGFTE
                     GDQLSRGAVDVVVTDGFSGNIALKTAEGTARFVTDLLRRAFTSSLRSKAGFALSRPAL
                     NLLKVHLDPNNHNGAVFLGLNGLVVKSHGSANPKGVANAIRVAATMVRNDITRKIGED
                     LDNFRAHAFADEAAQ"
     gene            complement(2122..2301)
                     /gene="rpmF"
                     /locus_tag="VFF72_13480"
     CDS             complement(2122..2301)
                     /gene="rpmF"
                     /locus_tag="VFF72_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZV94226.1"
                     /translation="MAVPKRKTSPSKRNMRRSHHALKATAFQECPNCGELKLPHNMCS
                     ACGHYNGREIVSTEA"
     gene            2496..>2751
                     /locus_tag="VFF72_13485"
     CDS             2496..>2751
                     /locus_tag="VFF72_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HZV94227.1"
                     /translation="MIAVPVFLAMTAAAALVNGWLVARTGKLRRQLRISVGDGGNDAL
                     LRRMRAQSNFLETTPFALFLVLGLELSGANRIALAIVGAIF"
BASE COUNT          453 a          879 c          901 g          518 t
ORIGIN      
        1 ccccgcctgg aacggcgtga cctcgccgtt tcgattacgt gttcgtaacg gaaatgcgct
       61 ccgtcggcaa tgcgcctaat catctgaaat tccaattttt tcaatacctg agcgcggccg
      121 cgccccaggt aaagccaccg cccatcgctt cgagcacgac gatgtcgccg cgcttgattc
      181 gcccgtcctt cacagcggtg tcgaacgcca gcggtacgga cgcggccgat gtattggcat
      241 gccggtcgac ggtgactact accttttgcg gcggcaggcc cagtttcttc gcggttgcgt
      301 cgagaatccg ggcgttcgcc tggtgcggaa cgacccagtc gacgtcatca gcaacgagcc
      361 cggcaccggc aagcacctcg ttcatcacgc cggcgaggtt gatcaccgca tgacggaaca
      421 cctcgcgtcc cttcatccgg agcttgccga ccgttccggt cgtcgagggt cccccgtcga
      481 cgaacagcag gtcgttgtgg cggccatcgg catgcagctt ggtcgagagg attccggatt
      541 cggtgtcctc ggcgctgagc accagtgcgc cggcgccgtc gccgaacagc acgcaggtcg
      601 cgcggtcttc ccaatcgagg atccggctga aggtctcggc gccgatcacg agtgccttgt
      661 ctgccccgcc cgagcgaagc atcgaatcgg cgaccgacag cgcgtagaga aagccggtgc
      721 acaccgcatg cacgtcgaag gcgatgcagt cgttgatccc aagcgcggct tggaccttgg
      781 tcgcggtcga cgggaaggtc tggtcgggag tcgccgtggc gaggacgatc aggctgattt
      841 cgcccgcatc gatcccggcg tcgtcgagcg cccgtcgagc ggcttcggtg gcgaggctgc
      901 cggtggtctc gccctccgac gcgatatagc ggctgcggat tcccgtccgc tcgacgatcc
      961 attcgtccga ggtgtcgacg gtggccgcaa gttcgtcgtt ggtgaccttg cgacggggga
     1021 gcgcgctgcc gactcctttg acgaccgcgc gtcgagtcac tgcgcagcct cgtctgcaaa
     1081 tgcatgcgca cggaaattgt cgaggtcctc gccgattttc cgggtgatgt cgttgcggac
     1141 catcgtcgcg gcgacccgaa tggcattggc gacgcccttc gggttggcac tgccatggct
     1201 tttcacgacg agaccgttga ggccgaggaa aaccgcgcca ttgtgattgt tggggtcgag
     1261 gtggaccttg agcaggttca gtgccggccg cgaaagcgcg aacccggcct tggagcgaag
     1321 cgagctcgtg aaggctcggc ggagcaggtc ggtgacgaac cgcgcggtac cctcggcagt
     1381 cttgagcgcg atgttgccgg aaaagccgtc ggtgacgacg acgtcgactg cgcctcgaga
     1441 cagctggtcg ccctcggtga agccgtcgaa ccggaacggc aaatagtccg cctcgcgcag
     1501 caaagcggcg gcgtccttga gctcgtcggt gcccttgagc tcctctgtgc cgatattcag
     1561 cagcttaacg cgcggcgccg cgatgcccag caccgtccgc gagtaagccg cgcccatgac
     1621 cgcgaactgg accaggttct gagcatcgca ttcggtgttc gcgccgaggt cgagcatgat
     1681 gacgtcgttg tcgccgagcg tcggaaggag cgctgcaagc gccggccggt cgattccggg
     1741 catggtgcgc agcgccagct tcgacatcgc catcatcgcg ccggtgtttc cgcccgacag
     1801 cgccgcgtcg gcgacgcctt ccttcacggc attgatcgcc atgcccatcg acgtcgtgcg
     1861 ggcgcgacgg atcgcctggc tgggcttctc actgggggcg atcgcttcgg gcgaatggac
     1921 cagctgcgaa cgtccggcga ggttgcggtg cacaccgatc tcggcggcaa cccgcacttc
     1981 gtcaccgtaa aatgtgaatt cgagcgtcgg atccttgcgc agtgcgtgcg atgcgccagc
     2041 gatcatcgcc ttcgggccgg catccccgcc catggcgtcg atcgcaatgc gcgggccccc
     2101 gctcatgggc ctcgggcccg cttaagcctc ggtcgagacg atttcgcggc cgttatagtg
     2161 gccgcatgcc gaacacatgt tgtgcggcag cttcagctcg ccgcagttcg ggcattcctg
     2221 gaacgccgtc gccttcaacg catgatggct gcggcgcatg ttccgcttgg acggcgaggt
     2281 ttttctcttg gggacagcca ttgcggcacc ttcaactgaa ttcgaccaac aataaaaatt
     2341 gcgacgatct gcgcctcatc tcggcagggc tcctgttggg gtgccccgcg ggcggcggcc
     2401 ggatcgtcgc gaaccgcggt ccctatagcg gacgtggccg atgttgcaag ctcgtgctgc
     2461 cgatgcgaga aacagccgta cggagggcca agtcaatgat cgccgttccc gtcttcctcg
     2521 caatgactgc cgcggcggcg ttggtgaacg gctggcttgt cgcgcggacc gggaagctcc
     2581 ggcgacagtt gaggatcagc gtcggcgacg gcgggaacga cgcattactg agacggatga
     2641 gggcgcaatc caacttcctt gaaacgacac ccttcgcgct gttcctggtg ctcggcctgg
     2701 agctgtcagg cgccaatcgg attgcgctgg caatcgtcgg cgcaatcttc a
//
LOCUS       DASRWS010000705         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2582114, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000705 DASRWS010000000
VERSION     DASRWS010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582114"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFF72_13490"
     CDS             <1..297
                     /locus_tag="VFF72_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZV94228.1"
                     /translation="FMGKYYREGDPKDNSNLYGYLAAQVMTQILKQCGNDLTRENVMR
                     QAANLKNFKLPLLLTGMTINTSPTDYAPIQQGQLARFTGTLWQAFGEVVSTGDQ"
     gene            402..2468
                     /gene="ppk1"
                     /locus_tag="VFF72_13495"
     CDS             402..2468
                     /gene="ppk1"
                     /locus_tag="VFF72_13495"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZV94229.1"
                     /translation="MTQTRAAAPGGGSPPLLYRERAILEFNRRVLAQARRVDVPLLER
                     LRYICIVSSNLDEFFEVRFAESVESSTLPGTGVGPADVEALSAAAHGLVAEQYAAFND
                     EVMPALASEGIRIVSHDDRSDAERRWVARFFEHEVRPLLVPVGLDPAHPFPLVANKSL
                     NFIARLAGKDAFGRRNTVTIVKVPRVLPRIVKLPGHGRRQSFALLTSIIRAHLGELFA
                     GREVEAFAQFRVTRDSDLEVDADVTNLRQALRSGLTTRHFGQAVRLEVVDTCPAEFSD
                     FLLRQFGLGATALYKAAGPVNLGRMTQLIDLADAPALRFAPFEPAWPASLYRQQSMFD
                     RLRAGDLLLHQPFESFEPVLQFLREAVYDPAVLAIKQTIYRAGSESALMDLLIEAARR
                     GKEVMAVVELQARFEEEANINWAERLEAVGAQVVYGVVGLKTHAKLLLVTRRENGRLK
                     RYAHLSTGNYNPVTARLYTDLGHFTADADLTADVDAVFQQLASLGRTPPLRVLLQSPF
                     TLHKQMLAQVARVAAAARAGGPARIVVKVNALTDVPLIEALVGAAKSGAAIDLIVRGA
                     CMLRPGDVDGKFPIRVRSVVGRFLEHSRVAYFRWGDHLEDEALFLSSADWMGRNMFGR
                     VEVAWPVRDAKLRQRVIDECLVPYLHDGLDAWTLDADGSYRRSVAGESAQQALVRLYA
                     KHPSPL"
     gene            2482..2955
                     /locus_tag="VFF72_13500"
     CDS             2482..2955
                     /locus_tag="VFF72_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZV94230.1"
                     /translation="MDLILWRHADATADGEDGDDLSRPLTARGARQAERMAGWLNERL
                     ADSTRILVSPALRCRQTAKALGRRSSIVAGLAPGASAETLLQLAGWPDAAGSVLVVGH
                     QPTLGLVASLVLEGIARSRSIRKAAVWWFRQRPRDADSQGAWHAVLHAVQSPEDL"
     gene            complement(3155..>3652)
                     /locus_tag="VFF72_13505"
     CDS             complement(3155..>3652)
                     /locus_tag="VFF72_13505"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZV94231.1"
                     /translation="RVGRVAAALFTGVLPRPNAESRRELRWAAALHEAGMMISHHDHH
                     RHSAYMLSHVDAEGFSQSQLRRVGDLVLGQRGGLRKLEPRLAEPGFAPALLCLRLAVI
                     VCHARDDVEAGAITLRIGDGPPTVTLRGEWAAAHPRAGHLLREEVAAWDRSGVLRPIL
                     QAQAA"
BASE COUNT          532 a         1296 c         1265 g          559 t
ORIGIN      
        1 ttcatgggca agtactaccg cgagggcgac ccgaaggaca actcgaacct gtacggctac
       61 ctcgctgcac aggtgatgac gcagatcctg aagcaatgcg gcaacgacct gacccgcgag
      121 aacgtcatga ggcaggcggc caacctgaag aacttcaagc tgccgttgct gctcaccggc
      181 atgacgatca acaccagccc gaccgactac gcgccgatcc agcaggggca gctcgcgcgc
      241 ttcaccggta cgctgtggca ggccttcggt gaggtagtca gcaccggcga ccagtagcgc
      301 ctggaacggc aggaggcgcc gctcgcactc ggcgctcgcg acgcccggcg gcgacggcgc
      361 cctctctcgg cgccgtcgaa catcctacat acactggcgc gatgacgcag acccgtgccg
      421 cggcgccggg cggcgggtcg ccgccgctcc tctaccgcga gcgcgccatc ctcgagttca
      481 accgccgcgt gctggcccag gcgcggcgcg tcgacgtgcc gctgctcgag cgattgcgct
      541 acatctgcat cgtctcgtcg aacctcgacg agttcttcga ggtccgcttc gccgagagcg
      601 tcgagtcgtc gacgctgccg gggaccggcg tcggcccggc cgatgtcgag gcgctgagcg
      661 cggctgcgca tggcctggtc gcggagcaat acgcggcgtt caacgacgaa gtgatgcccg
      721 cgctcgcgag cgagggcatc cgcatcgtca gccacgacga tcgcagcgac gccgaacggc
      781 gctgggtcgc gcgctttttc gagcacgagg tgcggccgct gctggtgccg gtcggactcg
      841 atccggccca tccgttcccg ctcgtcgcga acaagtcgct gaacttcatc gcccggctgg
      901 ccggcaagga cgcgttcggc cggcgcaaca cggtcacgat cgtcaaggtg ccgcgtgtcc
      961 tgccgcgcat cgtcaagctt ccggggcatg gacgccggca gtcgttcgca ctgctcacca
     1021 gcatcatccg cgcccacctg ggcgagctct tcgccggccg cgaggtcgag gccttcgcgc
     1081 agtttcgcgt cacccgcgat tccgatctcg aggtcgacgc cgacgtgacc aacctgcgcc
     1141 aggccttgcg ctcgggcctg acgacgcggc acttcggaca ggcggtgcgg ctcgaggtcg
     1201 tcgatacctg tccggccgag ttctccgatt tcctgctgcg ccagttcggg ctcggcgcga
     1261 cggcgctcta caaggcggcc gggccggtca acctggggcg catgacgcag ctgatcgatc
     1321 tcgccgacgc gccggcgctt cgcttcgcgc cgttcgagcc ggcgtggccg gcgagcctgt
     1381 atcggcagca gtcgatgttc gatcgcctgc gcgccggtga cttgctgctg caccagccgt
     1441 tcgagtcgtt cgagccggtg ctgcagttcc tgcgcgaggc ggtctacgac ccggcggtgc
     1501 tggcgatcaa gcagacgatc tatcgcgcgg gaagcgagtc ggcgctgatg gatctcctca
     1561 tcgaggcggc ccgtcgcggc aaggaggtga tggcggtcgt cgagctgcag gcccgcttcg
     1621 aggaggaggc caacatcaac tgggccgagc ggctcgaggc ggtcggggcg caggtggtct
     1681 acggcgtcgt cggcctgaag acgcatgcca agctgctgct cgtgacgcga cgcgagaacg
     1741 gcaggctgaa gcgctacgcg catctttcga ccggcaacta caacccggtc accgcgcgtc
     1801 tttacaccga cctcggccac ttcacggccg acgccgacct caccgccgac gtcgacgcgg
     1861 tgttccagca gctcgccagc ctcggccgca cgccgccgct gcgcgtcctg ctgcagtcgc
     1921 cgttcacgct gcacaagcag atgctggcgc aggtcgcgcg cgtcgctgcc gccgcgcggg
     1981 cgggcggccc ggcgcgcatc gtcgtcaagg tcaacgcgct caccgacgtg ccgctgatcg
     2041 aggcgctcgt cggcgcggcg aagtcgggag ccgcgatcga cctgatcgtt cgcggcgcct
     2101 gcatgctgcg gccaggcgac gtcgacggga aattccccat ccgcgtgcgc tcggtggtcg
     2161 gccgcttcct cgagcactcg cgcgtcgcct acttccgctg gggcgatcac cttgaagacg
     2221 aagcgctctt tctctcgagc gccgactgga tgggacgcaa catgttcggc cgcgtcgagg
     2281 tcgcctggcc ggtgcgcgac gcgaagctgc gccagcgcgt catcgacgaa tgcctcgttc
     2341 cctacctgca cgacgggctc gatgcgtgga cgctggacgc cgacggcagc taccgccgct
     2401 ctgtcgccgg cgaatcggcg cagcaggcgc tggtgcgcct ctacgcgaag cacccttcgc
     2461 ccctttgaaa cgagccggac gatggacctg atcctttggc gacatgccga tgcgaccgcc
     2521 gacggcgaag acggtgacga cctgtcgcgg ccgctcactg ccaggggcgc acggcaggcc
     2581 gagcgcatgg ccggatggtt gaacgagcgt ctcgcggatt cgacgcgaat cctggtcagc
     2641 cctgcgttgc gttgccggca gacggcgaag gcactgggtc gcaggagttc gatcgtcgcc
     2701 gggctcgcgc cgggcgcgag tgccgaaacg ctgctgcagc tcgctggctg gcccgacgcg
     2761 gccgggtcgg tgctcgtcgt cggccaccag ccgacgctcg gcctggtcgc ttcgctcgtg
     2821 ctcgaaggca tcgcccgatc gcggtcgatc cgcaaggcgg cggtgtggtg gttccggcaa
     2881 cgaccgcgcg acgccgacag ccagggcgcc tggcatgccg tgctgcatgc cgtgcagtcg
     2941 cccgaggatc tgtagcggcc ccaatcagtg aaaccggcgc cgcgacgtgc gcgggacgag
     3001 cgcatccctt gctgatcctt cgcgacgaac gcgccccctg ctcgcgtcgc tccgcgctcc
     3061 gggagtcgct cggtgctccg cgcttcgcgt ctcgaacgca tccgtcaccg catccgtcac
     3121 gggcatcccc gacgcgtgtg gcgagaatca cggctcaggc ggcctgcgcc tgaaggatgg
     3181 gtctcaggac gccggatcga tcccacgccg cgacctcttc gcgcagcagg tgcccggcgc
     3241 gcggatgcgc cgccgcccac tcgccgcgaa gcgtcactgt cggcggcccg tcgccgatgc
     3301 gcagggtgat ggcgccggcc tcgacgtcgt cgcgcgcgtg gcagacgatg accgccagcc
     3361 gcaggcagag gagggctggc gcgaagcccg gctccgccag tcgcggctcg agcttgcgca
     3421 ggccgccgcg ctggccgagg acgaggtcgc cgacgcgccg caactggctt tgcgagaagc
     3481 cctcggcgtc gacatgcgag agcatgtagg cgctgtgccg gtgatggtcg tggtgcgaaa
     3541 tcatcatgcc cgcctcgtgc agggccgcgg cccagcgcaa ctcgcgtcgc gactcggcgt
     3601 tcggccgggg cagcacgccg gtgaacaggg ctgccgcgac ccggccgacc cg
//
LOCUS       DASRWS010000706         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2001874, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000706 DASRWS010000000
VERSION     DASRWS010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001874"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFF72_13510"
     CDS             complement(<1..1056)
                     /locus_tag="VFF72_13510"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZV94232.1"
                     /translation="MGEPVFDVAIAGFGPSGAVAAALLGQAGHSVLVCDRSAEIYPLP
                     RAAALDHEILRVFQKLGLADAIEPHTESFTDSCFYGVEGQLIRRMTMLPPPHPQAWTP
                     SVVFNQPAVEAILRRSVAALPGVEVRLGTTVGGLAQDDDGVSLQFAEGGDVRARRVIA
                     CDGASSTVRGLLGIELEDLGFDEPWLVVDVLANEKGLARLSTHSAQYCEPDRPCSYII
                     GPKNHRRWEISLKEGEQPDDVLTEQGTWRLLERWIGPDEGELWRRATYRFHALVAQRW
                     RHGRVFLAGDAAHQQPPFLGQGMCQGIRDVVNLSWKLDAVLKGSAPESLLDTYGEERK
                     AHVRELTTRLKAIGTLIG"
     gene            complement(1060..1926)
                     /locus_tag="VFF72_13515"
     CDS             complement(1060..1926)
                     /locus_tag="VFF72_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZV94233.1"
                     /translation="MKFTTFLRDGEPRLGVVDGASVVDLNAARPDVPPDLREALESGI
                     DVVAAARAALASLAARIPVAGLAYAPVVPEPGKTICLGLNYFDHAKEGGREKPDYPWF
                     FYRGKSSLMGHGAPGLAPRVSIKFDYEAELAVVIGTRVPRHVSQAEALKHVFGYSCFN
                     DMSVRDFQKRTPQWTIGKNFDGTGGFGPVLVTADELAPGATGLRIQSRLNGQVMQDAN
                     TDDMIFSVAETIALLSECMTLEPGDAIIMGTPAGVGQARTPPVWMKAGDVIEIEIERV
                     GLLRNPIENEAA"
     gene            complement(1951..>2691)
                     /locus_tag="VFF72_13520"
     CDS             complement(1951..>2691)
                     /locus_tag="VFF72_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZV94234.1"
                     /translation="ALHEDKGPVAIRHVTLRARSREALDAIAQATVGAGGKVMSGPAS
                     LDGPAGGTGILIRDGDGRLIEVVHGDHRFEATGALRDRPTRLAHAVLNSHDVEATQRF
                     FERALGFSLADRTRIMAFLNCNRDHHSIALGDSDNDALNHIAFLMPDVESVMRGGGRM
                     KDAGFAIEWGPGRHGPGDNAFNYFIGPFGEVIEYTAEVQQVDETYRPGSPGDWTWPPG
                     RVDQWGISAPPSARLKQAQRAVLFVPSP"
BASE COUNT          401 a          893 c          962 g          435 t
ORIGIN      
        1 gccgatcagc gtgccgatcg ccttcagccg cgtcgtcagt tcacgaacgt gggccttgcg
       61 ttcctcgccg taggtgtcga gcagcgactc gggtgccgat cccttcagaa cggcgtcgag
      121 cttccacgac aggttgacga catcgcggat tccctggcac atgccctggc cgaggaacgg
      181 cggttgctga tgcgcggcgt cgccggcgag gaacaccctt ccgtggcgcc agcgctgtgc
      241 gaccagcgcg tggaagcgat aggtagcgcg acgccagagc tcgccctcgt cggggccgat
      301 ccagcgctcg aggagcctcc aggttccctg ctcggtcagg acgtcgtcgg gctgctcgcc
      361 ttccttcaac gagatctccc agcggcggtg attcttcggc ccgatgatgt aagagcacgg
      421 gcggtcgggc tcgcagtact gggcgctgtg cgtcgacagg cgcgccaggc ccttttcatt
      481 ggccaacacg tcgaccacca gccagggctc gtcgaagccg aggtcttcga gctcgatgcc
      541 gagcaggccg cggacggtgc tgctggcgcc gtcgcaggcg atgacccggc gggcccgcac
      601 gtcgccgccc tcggcgaatt gcagcgacac gccgtcgtcg tcctgggcca ggccgccgac
      661 ggtcgtcccg agccggacct cgacgccggg cagcgccgcg acggaccgtc gcaggatcgc
      721 ctccactgcc ggctggttga agacgaccga gggtgtccac gcttgcggat gcggtggcgg
      781 cagcatcgtc atgcgccgga tgagctggcc ctcgacgccg tagaagcagc tgtcggtgaa
      841 cgactcggta tgcggctcga tcgcgtcggc gagaccgagt ttctggaaga cgcgcaggat
      901 ctcgtggtcg agcgcggccg cacgtggcag cggataaatc tcggcgctgc gatcgcagac
      961 gagcacggag tggccggcct gcccgagcag cgccgctgcg accgccccgc tcggcccgaa
     1021 gccggcgatc gcgacgtcga agacgggctc gcccacgagt caggcggcct cgttctcgat
     1081 cggattgcgg agcaggccga cgcgctcgat ctcgatctcg atcacatcgc cggccttcat
     1141 ccacaccggc ggcgtgcgcg cctggccgac gcctgcgggc gtgcccatga tgatggcgtc
     1201 gcccggctcg agcgtcatgc attcggaaag cagggcgatg gtctcggcga cgctgaagat
     1261 catgtcgtcg gtgttggcgt cctgcatgac ctggccgttg aggcggctct ggatgcgaag
     1321 ccccgtggcg cccggcgcca actcgtcggc ggtgacgagc acggggccga agccgccggt
     1381 gccgtcgaag ttcttgccga tggtccattg cggcgtgcgc ttctggaagt cgcgcaccga
     1441 catgtcgttg aagcagctgt agccgaagac gtgcttcaag gcctcggcct gggagacgtg
     1501 ccgcggcacc cgcgtgccga tcaccaccgc gagctcggcc tcgtagtcga acttgatcga
     1561 caccctgggc gccagccccg gggcgccgtg acccatcagc gagctcttgc cgcgatagaa
     1621 gaaccacgga tagtcgggct tttcacggcc gccttccttg gcgtggtcga agtagttgag
     1681 gccgaggcag atcgtcttgc ccggctcggg cacgacgggg gcgtaggcga ggccggcgac
     1741 gggaatccgc gcggccagcg acgccagggc ggcacgtgcc gcggcaacga cgtcgatgcc
     1801 cgattcgagg gcctcgcgca ggtcgggcgg aacgtcgggc cgtgccgcgt tcaggtcgac
     1861 gacggatgca ccgtcgacga cgccgagccg aggctcgccg tcacgcagga aggtggtgaa
     1921 cttcatcggg attccttcat ggagattcct tcatggagat ggcacgaaca ggaccgcacg
     1981 ctgcgcctgc ttcaaccgtg cgctgggtgg cgccgagatg ccccactggt cgacccgtcc
     2041 gggcggccag gtccagtcgc ccgggcttcc ggggcggtag gtttcgtcga cctgctgcac
     2101 ctcggccgtg tattcgatga cctcgccgaa cgggccgatg aaatagttga aggcgttgtc
     2161 gccagggccg tggcggccgg ggccccattc gatggcgaag cccgcgtcct tcatccggcc
     2221 gccgccgcgc atcacggatt cgacgtcggg catcaggaac gcgatgtggt tgagcgcgtc
     2281 gttgtcggag tcgccgaggg cgatgctgtg gtgatcgcgg ttgcagttga ggaaggccat
     2341 gatccgggtg cgatcggcca gggaaaatcc gagcgcccgc tcgaagaagc gctgggtcgc
     2401 ttcgacgtcg tggctgttga gcaccgcgtg ggcgagtcgg gtcggtctgt cccgaagagc
     2461 gccggtcgcc tcgaagcgat ggtcgccgtg cacgacctcg atcagccggc cgtcgccgtc
     2521 gcgaatcagg atgcccgtgc cgccagccgg accgtcgagc gatgcagggc cggacatgac
     2581 cttgccgccg gcgccgacgg tggcctgcgc gatggcgtcc agcgcttccc ggctgcgcgc
     2641 ccgcagggtc acgtgccgga tggcgaccgg ccccttgtcc tcgtgcaacg c
//
LOCUS       DASRWS010000707         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2582624, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000707 DASRWS010000000
VERSION     DASRWS010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582624"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..504)
                     /locus_tag="VFF72_13525"
     CDS             complement(79..504)
                     /locus_tag="VFF72_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZV94235.1"
                     /translation="MPAVSLRGTRRRTINEINMVPFIDVMLVLLIIFMVTAPLITTGV
                     VDLPSVGRSASRPEHFIELVIGTDETLKMRVDGKDSAVLSPRSVGARVLEAQAGNAHT
                     PVVISADRGVKYDVVVRTMDALQRAGVQRVGLSVKQASS"
     gene            complement(641..1327)
                     /gene="tolQ"
                     /locus_tag="VFF72_13530"
     CDS             complement(641..1327)
                     /gene="tolQ"
                     /locus_tag="VFF72_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZV94236.1"
                     /translation="MNQQDFSIVTLAVHASLVVQVVMAGLLVASLASWTVIFGKLFGL
                     RKVRAANDEFEREFWAGRNLNDLYDEATRKAARSPMERIFASGMREFFKLREKRVVDA
                     GALLDGARRAMRASFQRELDTIESNLSFLASVGSVSPYVGLFGTVWGIMHAFIGLSNL
                     QQVTLATVAPGIAEALVATAIGLFAAIPAVIAYNRFARDIDRIAIQLESFIEEFSNIL
                     QRNAGALPGK"
     gene            complement(1324..1779)
                     /gene="ybgC"
                     /locus_tag="VFF72_13535"
     CDS             complement(1324..1779)
                     /gene="ybgC"
                     /locus_tag="VFF72_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HZV94237.1"
                     /translation="MSLRRRHAAARTPVSFRHPIRIYWEDTDAAGIVFYANYLKFFER
                     ARTEWLRSLGFDQERLRADDGIAFVVTETTLRYRLPARLDDMLEVTVRVVHGGQASLE
                     VAQEAWRAGDLLAEGSIRVGCVGLGTFRPRRVPDSILAKIRTSPHQQQQ"
     gene            complement(1804..2193)
                     /locus_tag="VFF72_13540"
     CDS             complement(1804..2193)
                     /locus_tag="VFF72_13540"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HZV94238.1"
                     /translation="MQRGLAAIASAACLFVSACAQPAPPLGLLDVTARPAEHDLLLGL
                     RAYDDAQYEAARRLFGRALAQGLVSPADRAEAHKRLAFIDCAAGRLAECEAEFRLARQ
                     ADGHFVLSPSEAGHPVWGPVYRKVVPN"
     gene            complement(2205..>2743)
                     /locus_tag="VFF72_13545"
     CDS             complement(2205..>2743)
                     /locus_tag="VFF72_13545"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZV94239.1"
                     /translation="AAPPAAASSINASDALLLATLVPTPMPPASADRANAPARPLAAP
                     PASLSAPATAPHRDAPSGNGVATRGHTGTAAAPQRESARDRRAREAREARDREVASRA
                     PAPVAPATGTVHIAISPWGQVEVDGAPSGAAPPLTELTLPEGRHRIVVRNGDFAPYVT
                     SVNVTGGQTVSLRYKFGS"
BASE COUNT          423 a          888 c         1032 g          400 t
ORIGIN      
        1 cagcgcatgc ccggcggccg gcgcggcgcg aaggcatcgt gggcgaggac ggtggcgggg
       61 ctcatcgcgc ggcgttcgtc agctcgaggc ctgcttgacc gacaggccga cgcgctgcac
      121 gccggcgcgc tgcagcgcgt ccatcgttcg cacgacgacg tcgtacttca cgccgcgatc
      181 ggccgagatc accaccggcg tgtgcgcgtt gccggcctgc gcctcgagaa cgcgggcgcc
      241 gacgctgcgc ggcgagagca cggccgagtc cttgccgtcg acgcgcatct tcagcgtctc
      301 gtcggtgccg atcaccagct cgatgaaatg ctccggccgg ctggcgctgc gcccgacgct
      361 gggcaggtcg acgacgccgg tggtgatgag cggcgcggtc accatgaaaa tgatcagcag
      421 caccagcatg acgtcgatga acgggaccat gttgatctcg ttgatcgtcc ggcggcgcgt
      481 gccgcgaagg ctcaccgcgg gcatgagaat tcttttggcc gcgccccctc aaggggcagc
      541 gagcgaagag agcgtggggg caccggccgg tcgccgggcc gccccaaggc cggccgcgcc
      601 ccctcggggg gcagcgaacg aagagagcgt gggggcacca tcatttcccg ggcagagcgc
      661 ccgcgttgcg ctgcaggatg ttcgagaact cctcgatgaa gctctcgagc tggatggcga
      721 tccggtcgat gtcgcgggcg aagcggttgt aggcgatcac cgccgggatc gccgcgaata
      781 ggccgatcgc ggtggcgacc agcgcctcgg cgatgccggg cgcgacggtg gcgagcgtca
      841 cctgctgcag gttcgacagg ccgatgaagg cgtgcatgat tccccagacc gtgccgaaca
      901 gtccgacgta gggcgatacc gatccgaccg aagcgaggaa ggaaaggttc gattcgatcg
      961 tgtcgagttc gcgctggaag ctggctcgca tcgcccggcg ggcgccgtcg aggagcgcgc
     1021 cggcatcgac gacgcgcttt tcgcgcagct tgaagaactc gcgcatgccc gaagcgaaga
     1081 tgcgctccat cggcgatcgt gccgccttgc gtgtcgcctc gtcgtagagg tcgttgaggt
     1141 tgcgtccggc ccagaactcg cgctcgaact cgtcgttggc ggcgcgcacc ttgcgcagtc
     1201 cgaatagctt gccgaagatc accgtccagc ttgccaggga ggcgacgagg aggccggcca
     1261 tcaccacctg gacgaccagg ctggcgtgga cggcgagggt gacgatcgag aaatcctgct
     1321 ggttcattgt tgttgttggt gcggcgaagt acggattttc gcgaggatcg agtcaggcac
     1381 gcggcgcggc cggaaagttc cgaggccgac gcaaccgacc cggatgctgc cctcggccag
     1441 caaatcgccg gcccgccagg cctcctgggc cacctcgagg ctggcctggc cgccgtggac
     1501 gacacggacg gtgacctcga gcatatcgtc cagccgcgcc ggcaaccggt agcgcagcgt
     1561 ggtttcggtg acgacgaagg cgatgccgtc gtcggcgcgc agtcgttcct ggtcgaagcc
     1621 gaggctgcgc agccattcgg tccgtgcccg ttcgaagaac ttcaggtagt tggcgtagaa
     1681 gacgatgccc gccgcgtcgg tgtcttccca gtagatgcgg atcggatggc gaaaggatac
     1741 cggcgtcctc gccgcggcat ggcgccgacg caagctcatg agccgccggc gccggccatg
     1801 ccgtcagttc gggacgacct tgcggtagac gggaccccag accgggtggc cggcctcgga
     1861 cgggctgaga acgaaatggc cgtcggcctg gcgagcgagg cggaattcgg cttcgcattc
     1921 ggccaatcgc ccggcggcgc aatcgatgaa ggcgagccgc ttgtgcgctt cggcgcgatc
     1981 ggcgggcgag acgagcccct gcgccagggc ccgcccgaag aggcgccggg cggcttcgta
     2041 ctgggcatcg tcatacgcac gcaggcccag cagcaggtcg tgttccgccg gccgggcggt
     2101 gacatcgagc aagccgagcg gcggcgcagg ctgggcgcag gcagaaacga agaggcatgc
     2161 ggccgatgct atcgcggcga ggcctcgctg catcgcgtcc gtcctcagga accgaacttg
     2221 tagcgaagcg agacggtctg gccgccagtg acgttgacgg aggtcacgta tggcgcgaag
     2281 tcgccgttgc ggacgacgat gcgatgcctg ccctcgggca gcgtcagctc ggtgagcgga
     2341 ggtgccgctc cggacggcgc gccatcgacc tcgacctgtc cccacgggct gatggcgatg
     2401 tgaaccgtgc cggtggccgg tgcgaccggc gccggggcac gactggcgac ctcgcgatcc
     2461 cgggcttcgc gtgcctcgcg ggcacggcgg tcgcgtgccg actcgcgctg cggcgcggcg
     2521 gcggtgccgg tatggccgcg ggtggcgact ccgttgccgg agggagcgtc gcggtgcggt
     2581 gccgttgccg gcgcagacag cgacgcgggc ggtgccgcga ggggacgggc tggcgcgttg
     2641 gccctgtccg cagacgccgg cggcatcggc gtcggcacca gcgtcgcgag cagcagggcg
     2701 tcgctggcgt tgatcgacga cgcggccgcg ggcggcgcgg ccg
//
LOCUS       DASRWS010000708         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_572580, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000708 DASRWS010000000
VERSION     DASRWS010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572580"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFF72_13550"
     CDS             <1..911
                     /locus_tag="VFF72_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HZV94240.1"
                     /translation="LAFNFSQLPNSGVGLARLEFIINNNIGVHPKAILDYPNIDVDLK
                     KAVESVARGHASPRAFYVDKLAEGVATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRY
                     EPDEENPMLGFRGASRYISGEFSDAFAMECEALKRVRHDMGLTNVEIMVPFVRTLRQA
                     EQVVGMLAEQGLRRATAGGNDGLKIIMMCEVPSNAILAEQFLQHFDGMSIGSNDLTQL
                     TLGLDRDSGMELLARDFDERDPAVKALISRTIAACRAIGKYVGICGQGPSDHADFADW
                     LADEGIGSISLNPDSVVETWKRLATR"
     gene            complement(924..1451)
                     /locus_tag="VFF72_13555"
     CDS             complement(924..1451)
                     /locus_tag="VFF72_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZV94241.1"
                     /translation="MTAVAEIRPLVAADLRDYKQLRDEMLLAHPEAFTSDAAEEKSKE
                     PADYLYRLGLDRRGAGGHFLLGAWRGERLIGAIGCERELRAKVRHIGHVVGLMVRRDL
                     RGRGVGRELLDACIGEARRTEGIELLVLTVTSGNEAAVRLYQGCGFIAYGTLPRAVRI
                     GHIYHDKLHMALRLR"
     gene            complement(1474..>2514)
                     /gene="moaA"
                     /locus_tag="VFF72_13560"
     CDS             complement(1474..>2514)
                     /gene="moaA"
                     /locus_tag="VFF72_13560"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZV94242.1"
                     /translation="SGELIDRLQRPLHDLRISVTDRCNFRCTYCMPKEVFDKNYAFLP
                     QSSLLSFEEITRLAAVFVAHGVRKIRLTGGEPLLRRHLERLVAMLAELRDVDGEPLDL
                     TLTTNGSLLARKARAMKDAGLKRVTVSLDALDDSIFRSLNDADFPVDDVLAGIDAAEE
                     AGLAPVKINMVVKRGTNDGEILPMVRRFRGRNVALRFIEFMDVGSTNGWRMDEVLPSA
                     RLIEQIGEAFALLPVATSTVGETAERWRYAEAGVAGEIGTISSVTRAFCRDCNRARLS
                     TEGKLFLCLFASRGHDLRALLRNGASDAEIAAAIGLIWQGRDDRYSELRGRAVAPAGT
                     AERRVEMHYIGG"
BASE COUNT          415 a          858 c          848 g          393 t
ORIGIN      
        1 agctggcgtt caacttctcg cagctgccca actccggcgt cggcctggcg cgcctggagt
       61 tcatcatcaa caacaacatc ggcgtgcacc cgaaggcgat cctcgactac ccgaacatcg
      121 acgtcgacct gaagaaggcc gtcgagtcgg tggcgcgcgg ccacgcgtcg ccgcgcgcgt
      181 tctacgtcga caagctcgcc gagggcgtgg ccaccatcgc cgccgcgttc tggcccaagc
      241 cggtcatcgt gcgcctgtcg gacttcaagt ccaacgagta caagaagctg atcggcggct
      301 cgcgctacga gccggacgaa gagaacccga tgctgggctt ccgcggcgcg tcgcgctaca
      361 tcagcggcga gttcagcgac gccttcgcga tggagtgcga ggcgctcaag cgcgtgcgcc
      421 acgacatggg cctgaccaac gtcgagatca tggtcccgtt cgtgcggacg ctgcgccagg
      481 ccgagcaggt cgtcggcatg ctcgccgagc aggggctcag gcgcgccacg gcaggcggca
      541 acgacggcct gaagatcatc atgatgtgcg aggtcccgag caatgcgatc ctcgccgagc
      601 agttcctgca gcactttgac ggcatgtcga tcggctccaa cgacctgacc cagctcacgc
      661 tcgggctcga ccgcgactcc ggcatggaat tgctggcgcg cgatttcgac gagcgagacc
      721 cggcggtcaa ggcgctgatc tcgcgcacca tcgccgcctg ccgcgccatc ggcaagtacg
      781 tcggcatctg cggccagggg ccgagcgatc acgccgactt cgccgactgg ctcgccgacg
      841 agggcatcgg ttcgatctcg ctcaatcccg actccgtggt cgagacgtgg aagcggctgg
      901 cgacgcgctg aatccgacct cggtcagcgc agcctcaagg ccatgtgcag cttgtcgtga
      961 tagatgtggc cgatcctgac cgcacgcggg agggtgccgt acgcgatgaa gccgcaacct
     1021 tggtagaggc gcaccgccgc ctcgttgcct gaggtcacgg tcaggaccag caattcgatg
     1081 ccctcggttc gccgggcttc gccgatgcag gcgtcgagca gctcgcgtcc gacgccacgg
     1141 cctcgcaggt cgcggcgcac catcaggccg acgacgtggc cgatatgccg gaccttggcg
     1201 cgcagctcgc gctcgcaacc gatcgcaccg atcagccgct cgccgcgcca tgcgccgagc
     1261 aggaagtggc cgcccgcgcc acggcggtcg aggcccaggc gatagaggta gtcggcgggc
     1321 tccttggact tttcctcggc ggcatccgag gtgaacgctt cgggatgggc gagcagcatc
     1381 tcgtcgcgca gctgcttgta gtcgcgcaga tcggccgcca cgagcggacg gatctcggcg
     1441 accgcggtca tggtggcgcg ttgccggtgc gcttcagccg ccgatgtagt gcatctcgac
     1501 gcgccgttcg gccgtgccgg cgggcgcgac ggcgcggccg cgcagctcgg aatagcggtc
     1561 gtcccgaccc tgccagatca ggccgatcgc cgcggcgatc tcggcgtcgc tggcgccgtt
     1621 gcgcaggagc gccctgaggt cgtggccgcg gctcgcgaag aggcacagga agagcttgcc
     1681 ttccgtcgac agacgggccc ggttgcagtc gcggcagaac gcgcgggtga cgctcgagat
     1741 cgtgccgatc tcgccggcga cgccggcttc ggcgtagcgc cagcgctcgg ccgtctcgcc
     1801 gacggtcgac gtcgcgaccg gaagcagcgc gaatgcctcg ccgatctgct cgatcagccg
     1861 cgccgaaggc agcacctcgt ccatccgcca gccgttggtg ctgccaacgt ccatgaactc
     1921 gatgaagcga agcgccacgt ttcggccacg aaagcgccgc accatcggca ggatctcgcc
     1981 gtcgttggtg ccgcgcttga cgaccatgtt gatctttacc ggcgccaagc cggcctcctc
     2041 ggcggcgtcg atgccggcga gcacgtcgtc gaccgggaag tcggcgtcgt tgaggctgcg
     2101 aaagatcgaa tcgtcgaggg cgtcgaggct caccgtcacg cgtttgagcc cagcgtcctt
     2161 catcgcccgc gccttgcggg cgagcagcga gccgttcgtc gtcaaggtca gatcgagcgg
     2221 ctcgccgtcg acatcccgca attcggcgag catggcgacc aggcgctcga gatggcgacg
     2281 cagcagcggc tcgccgccgg tgaggcggat ctttcgcacg ccatgggcca cgaacaccgc
     2341 ggcgagccgg gtgatctcct cgaagctcag cagcgacgat tgcggcagga aggcgtagtt
     2401 cttgtcgaag acctccttgg gcatgcaata cgtgcaacga aagttgcacc ggtcggtcac
     2461 cgaaatccgc aagtcgtgca gcggccgctg caatcggtcg atcagctcgc cact
//
LOCUS       DASRWS010000709         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2583653, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000709 DASRWS010000000
VERSION     DASRWS010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583653"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFF72_13565"
     CDS             <1..424
                     /locus_tag="VFF72_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94243.1"
                     /translation="IIGTWRFTFISDGNAYPKAIGYGETVDFGTSQWHRDGTELMISG
                     GRAPSTGDVCMGSWQQTGPNTYRLKHIALAYVSPDSLPPAPGAAYLGPVIIRENVTVN
                     RARNTFEGTFTLDQYARDEVTLLEHISGNVTATRFGVD"
     gene            544..1731
                     /locus_tag="VFF72_13570"
     CDS             544..1731
                     /locus_tag="VFF72_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV94244.1"
                     /translation="MQRRPALHAVLASAVLAAFAALPARAAGQTPFKIGFILPMTGQQ
                     ASTGKQIDAAVKLYMAQNGTTVDGRKIELIVKDDGGAPDMTKRIAQEMVVGDKVDVLA
                     GFGITPSAFAAAPIATQSKTPMVVTAAATSAITERSPYIVRTSFTLPQATVPIADWAA
                     KNGIKKVVTFVADYGPGLDAEKFFKSQFTLDGGQVVGEIRTPLKSPDFAPFLQKVADL
                     KPDAVFVFLPSGQGAAFMKQFGERGLDKSGIRLIATGDVTDDDQLADMGNVALGVVTS
                     HHYSAAHPSAVNKKFVAAFEAANHFRPNFMAVAGYDGMHLIYNALAATQGKGGGDALL
                     AAMKGQIFESPRGPILIDAQTRDIVQDIYIRKVERVPAMGNQLYNVEFDVQKAVKDPG
                     KTK"
     gene            complement(1829..>2608)
                     /locus_tag="VFF72_13575"
     CDS             complement(1829..>2608)
                     /locus_tag="VFF72_13575"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HZV94245.1"
                     /translation="VMAWKAGPAGQGPVLDLGERTSRGPIVEAALKQASRPVRPQAAR
                     SRPKSIDRRAATEIASAPKNVVRDEPPEHWCAEVFDAIEWRRFESVVEALFAQAGFET
                     RSQPSGADGGIDIWLHSRALGGRLVSLVQCKHWSSWKVGVKPVRELRGVMGAHRIARG
                     QFVTTSRYTADARLFARDNGIMLHDTRSLLELIASRSDEEQQALLRVAFFGEYWRPTC
                     ASCGVKLVERQAHDGSREPFWGCRNYPRCRRTLPMRPMPTS"
BASE COUNT          437 a          927 c          855 g          389 t
ORIGIN      
        1 catcatcggt acctggcgct ttaccttcat ctccgacggc aacgcctacc ccaaggcgat
       61 aggctacggg gagacggtcg atttcggcac gtcgcaatgg caccgcgacg gcaccgagct
      121 gatgatttcc ggaggccggg cgccgagcac gggcgacgtg tgcatgggca gctggcagca
      181 gaccggcccg aacacctatc ggctcaagca catcgccctc gcctacgtca gccccgactc
      241 gttgccgccg gcgcccggcg ccgcgtatct gggaccggtc atcatccgcg agaacgtgac
      301 cgtcaaccgt gcgcgcaaca ccttcgaagg gaccttcacg ctcgatcaat acgccaggga
      361 cgaggtcacg ttgctcgagc acatcagcgg caacgtgaca gcgacgcgct tcggcgtcga
      421 ctgacgtcgc gccgcgcggg ccggcatcgg ccggcccggc gccggttggc gaatatcccg
      481 atggtaggcg tcgtgcgaag ggcaacaatc ccgggcaact ccttcgcgac atccggattc
      541 accatgcaaa gacgccccgc cctccatgcc gttctcgctt cggccgtcct cgccgccttc
      601 gccgccctgc cggcacgggc tgccggccag acgccgttca agatcggctt catcctgccc
      661 atgaccggcc agcaggcgtc gacgggcaag cagatcgacg cggcggtcaa gctctacatg
      721 gcgcaaaacg gcaccaccgt cgatggccgg aagatcgagc tcatcgtcaa ggacgacggc
      781 ggcgcgcccg acatgaccaa gcgcatcgcc caggagatgg tcgtgggcga caaggtcgac
      841 gtgctcgccg gcttcggcat cacgccgtcg gccttcgccg ccgcgccgat cgcgacgcag
      901 agcaagacgc cgatggtcgt caccgccgcg gcgacgtcgg ccatcaccga gcgctcgccc
      961 tacatcgtgc gcaccagctt caccctgccg caggcgaccg tgccgatcgc cgactgggcg
     1021 gccaagaacg gcatcaagaa ggtcgtcacc ttcgtcgccg actacggccc ggggctcgac
     1081 gccgagaagt tcttcaagag ccagttcacc ctcgacggcg gccaggtggt gggcgagatc
     1141 cgcacgccgc tgaagagccc cgacttcgcg cccttcctgc agaaggtggc cgacctcaag
     1201 cccgacgccg tgttcgtctt cctgccgtcg ggacagggcg cggcgttcat gaagcagttc
     1261 ggcgagcgcg gcctcgacaa gtcggggatc cgcctcatcg ccaccggcga cgtcacggac
     1321 gacgaccagc tcgccgacat gggcaacgtc gcgctcggcg tcgtcacctc gcaccactat
     1381 tcggcggcgc atccgtcggc ggtgaacaag aagttcgtcg ccgccttcga ggccgccaac
     1441 cacttccggc ccaacttcat ggccgtcgcc gggtacgacg gcatgcacct catctacaac
     1501 gccctggccg cgacccaggg caagggcggc ggcgatgcgc tgctggcggc gatgaagggg
     1561 cagatcttcg aaagcccgcg cggcccgatc ctgatcgatg cgcagacgcg cgacatcgtc
     1621 caggacatct acatcaggaa ggtcgagcgt gtgccggcga tgggaaacca gctctacaac
     1681 gtcgagttcg acgttcagaa ggcggtgaag gatccgggca agacgaaata ggcgccggcg
     1741 tcggcgctcg ccgatgccag gcccggcgac gtctcacctg cggcgaacgc gttttccccg
     1801 ctgcgtcgag taccgccgcg acggacggtc agctggtcgg catcggccgc atcggcagcg
     1861 tccgccggca gcgcgggtag ttgcgacagc cccagaatgg ctcgcggctg ccgtcgtgcg
     1921 cctgtcgctc gaccagcttg acgccgcagc tcgcgcaggt cggtcgccag tactcgccga
     1981 agaaggccac ccgcaaaagc gcctgttgtt cctcgtcgct gcggctggcg atcagctcga
     2041 gcaggctcct cgtgtcgtgc agcatgatgc cgttgtcgcg ggcgaagagg cgggcgtcgg
     2101 cggtgtagcg cgatgtcgtc acgaactggc cgcgcgcgat ccggtgcgcc cccatcacgc
     2161 cgcgcaactc gcgcaccggt ttgacgccga ccttccagct cgaccagtgc ttgcactgga
     2221 cgaggctgac gaggcggccg ccgagcgccc gggagtgaag ccagatgtcg atgccgccgt
     2281 cggcgccgct gggttgcgag cgcgtctcga atccggcctg cgcgaagagg gcttcgacga
     2341 ccgattcgaa gcggcgccac tcgatcgcat cgaagacctc ggcacaccag tgctcgggcg
     2401 gttcgtctcg tacgacgttc ttcggcgcag aggcgatttc ggtcgcggcc cggcgatcga
     2461 tgctcttcgg gcgcgagcgc gctgcctgcg gccgcaccgg acgcgacgcc tgcttcaacg
     2521 cggcttcgac gatcgggccg cgcgaggtgc gctcacccag gtcgagaacg gggccttggc
     2581 ccgccggtcc tgccttccac gccatgac
//
LOCUS       DASRWS010000710         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3158845, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000710 DASRWS010000000
VERSION     DASRWS010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3158845"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..733
                     /locus_tag="VFF72_13580"
                     /pseudo
     CDS             209..733
                     /locus_tag="VFF72_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
     gene            852..2042
                     /locus_tag="VFF72_13585"
     CDS             852..2042
                     /locus_tag="VFF72_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZV94246.1"
                     /translation="MSSGNMERVLRLMSEKGASDVYLSASSPILIKINGQLLQLSDQP
                     LTHAQPRQLLAELLTPSQLEELEDTGELNVGVGLSGVGSFRLSGFKQRGSIAAVFRCI
                     PFEIPTLESLNVPPVLSTLVMEKRGLILMVGATGAGKSTTLASMIEQRNQQMAGHILT
                     IEDPIEFLFNNKKSVVNQREVGRDTQTLQIGLKTALRQAPDLIMIGEIRDRETMTAAI
                     SYALSGHLVLSTLHANNSYHALGRILSFYSPEARPALLADLAAGLRAIVSQRLVRSVA
                     GGRMPAVEVLLNTKLIQELIERGDFSGVKEAMEKSMAEGSQSFEGDLARMINQNIITR
                     DEGLAYADSPTNLMWRLQNDMSPVSRIVPKKEENDDQPSFTEITIDVRPEEVTGLSPN
                     VRRF"
     gene            2056..>2295
                     /locus_tag="VFF72_13590"
     CDS             2056..>2295
                     /locus_tag="VFF72_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347539.1"
                     /note="dapE-encoded N-succinyl-L,L-diaminopimelic acid
                     desuccinylase (DapE), catalyzes the hydrolysis of
                     N-succinyl-L,Ldiaminopimelate L,L-SDAP to
                     L,L-diaminopimelate and succinate. It is a metalloprotease
                     containing dinuclear active sites. Its structure is
                     similar to the carboxypeptidase G2 from Pseudomonas sp.
                     strain RS-16 and the aminopeptidase from Aeromonas
                     proteolytica; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZV94247.1"
                     /translation="MASSTLRLASQLIERRSVTPDDGGCQALLADRLARLGFDCIPLP
                     FGSDAARVDNLWAVRAGRGGKLLVFAGHTDVVPTGP"
BASE COUNT          386 a          794 c          758 g          357 t
ORIGIN      
        1 tcggcgcgag gcggagcagc gtgccgccgc tgtcggagag atagatcgtg ccgtcgggta
       61 cgagcgcgag atcgccgagt tgggcgccct tcgggccatc gatgcgttgc tgcaacgcgc
      121 cggtcgcgag gtcgtagctc agcagcgcgt tctcgtcggg cgggccacca tcgggatgga
      181 gttcggcagc agcgacgaga aggcgatgcg ggccaccggg gtgcgcgtcg tcccgccggc
      241 ggtggcgcgc cgcggttcct tcgcggcgaa gaacgtcgtg ctgatgccga gctacgtgaa
      301 catcggcgcc tacatcgacg aaggcaccat ggtcgacacc tgggccaccg tcggctcgtg
      361 cgcgcagatc ggcaagggcg ttcacctctc gggcggcgtc ggcatcggcg gcgtgctcga
      421 accgatgcag gccaacccga ccatcatcga ggacaactgc ttcatcggcg cccgctcgga
      481 gatcgtcgag ggcgtgatcg tcgaggagaa ctcggtcgtc tcgatgggtg tctatatcag
      541 ccagagcacc aagatctacg accgcgccac cggcacggtg agctacggcc gcattccgtc
      601 gggctcggtc gtcgtcagcg gcaacctgcc ggcagccgac ggtagccaca gcctgtactg
      661 cgccgtcatc gtcaagcgcg tcgacgcgca gactcgcgcc aagacgagca tcaacgaact
      721 gctgcgatca tgaattcccc acgccctgtt cgctcgcctt cgctcgttcc ggtcgctgcg
      781 ctccgtgcgg gcgcggccgg gtgcgcgagc cgccccgtgc ccagccggtt cacctgtgag
      841 aggacatcgc catgagtagc ggcaacatgg agcgcgtgct ccgcctgatg tccgagaagg
      901 gcgcatcgga cgtctacctc tcggcgagct cgccgatcct gatcaagatc aatggccagc
      961 tgcttcagct ctccgaccag ccgctgaccc acgcccagcc gcgccagctg ctggccgagc
     1021 tgctcacgcc gagccagctc gaagagctcg aggacaccgg cgagctcaac gtcggcgtgg
     1081 gcctttccgg cgtcggcagc tttcgtctct cgggattcaa gcagcgcggc tcgatcgcgg
     1141 ccgtctttcg ctgcatcccg ttcgagatcc cgaccctcga gagcctcaac gtgccgcccg
     1201 tcctcagcac gctggtgatg gaaaagcgcg gcctcatcct gatggtgggc gcgaccggcg
     1261 ccggcaagag cacgacgctg gcatcgatga tcgagcagcg caaccagcag atggccgggc
     1321 acatcctcac catcgaggat ccgatcgagt tcctgttcaa caacaagaag tcggtggtca
     1381 accagcgcga ggtcggccgc gacacccaga cgctgcagat cggcctgaag accgcgctgc
     1441 gccaggcgcc cgacctgatc atgatcggcg agatccgcga tcgcgagacg atgacggcag
     1501 cgatctcgta cgcgctgtcg ggccacctcg tcctgtcgac cctgcacgcc aacaacagct
     1561 accacgcgct cggccggatc ctgtcgttct attcgcccga ggcgcgtccg gcgctgctcg
     1621 ccgacctcgc ggcgggcttg cgcgcgatcg tctcgcaacg cctcgtgcgc agcgtcgccg
     1681 gcggccgcat gccggcggtc gaagtgctgc tcaacaccaa gctgatccag gagctcatcg
     1741 agcgcggcga tttcagtggc gtcaaggagg cgatggaaaa gtcgatggcc gagggctcgc
     1801 agagcttcga gggcgacctg gcgcgaatga tcaaccagaa catcatcacc cgggacgaag
     1861 ggctggccta cgccgattcg ccgaccaacc tgatgtggcg gcttcagaac gacatgtcgc
     1921 cggtgtcgcg gatcgtcccg aagaaggaag aaaacgacga ccagccgagc ttcaccgaga
     1981 tcacgatcga cgtgcgtccc gaggaagtga ccggcctgtc gcccaacgtt cgccgcttct
     2041 gacccgggcc cggccgtggc gtcgtcgacg ctccgtctgg cctcgcagct gatcgaacgg
     2101 cgctccgtga cgcccgacga cggcggctgc caggccctgc tggccgaccg actggcgcgt
     2161 ctcggcttcg attgcatccc gctgcccttc ggcagcgacg cggcccgcgt cgacaacctc
     2221 tgggcggtga gagcgggccg cggcggcaag ctgctggtct tcgccggcca caccgacgtc
     2281 gtcccgaccg ggccg
//
LOCUS       DASRWS010000711         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2004880, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000711 DASRWS010000000
VERSION     DASRWS010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004880"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="ccsA"
                     /locus_tag="VFF72_13595"
     CDS             complement(<1..464)
                     /gene="ccsA"
                     /locus_tag="VFF72_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZV94248.1"
                     /translation="MTTLRLFHFAAPARFYRLAGVLVPICWAAFAIFAIAGLYIGFFR
                     APTDATQGDAYRIIFIHVPAAWMSMVLYIAMAFWAAIGWAFNARLASMLARAIAPSGA
                     LFTFLALWTGSFWGKPAWGTWWVWDARLTSELILLFLYAGFLAIVAAIDDPRR"
     gene            complement(520..1194)
                     /gene="ccmB"
                     /locus_tag="VFF72_13600"
     CDS             complement(520..1194)
                     /gene="ccmB"
                     /locus_tag="VFF72_13600"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZV94249.1"
                     /translation="MSGVFAAVVGRDLRLAARRPADALLPIAFFTVAISLFPLGVGPE
                     AQTLRQIAPGVVWVAALLAAMISVTQLYAADQADGSLEQLLLAEPAGLALELVGAKAT
                     AHWLVTGLPLVIAAPLFGLMFDMRGEAIAMLAATLLLGTPVLSLLGGLGAALTLGLRS
                     GAALLILLIVPLCIPALIFGAGAVAAIDDGLSARGQLSLLGAFLLFTTLVAPPATSAA
                     LRIATE"
     gene            complement(1191..1847)
                     /gene="ccmA"
                     /locus_tag="VFF72_13605"
     CDS             complement(1191..1847)
                     /gene="ccmA"
                     /locus_tag="VFF72_13605"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZV94250.1"
                     /translation="MSAPVDRNDMPVLAVQGLACRRGERELFRPVDLDVRAGQIVWVR
                     GANGQGKTTLLRTLAGLCPVETGRIVWAQPRRRVVYLAHANALKDDLSVAEALAFLLR
                     IGGVRPTAGELSTALEAFAMSGRADAPVRTLSQGQRRRVALARLAHEREASAWLLDEP
                     FDALDAAGIESLNALLAAHARRGGSVVLTSHVPLSIDSPRPFAVMLEAPLVAVAPAPA
                     "
BASE COUNT          357 a          671 c          787 g          223 t
ORIGIN      
        1 cggcgcggat cgtcgatcgc ggcgacgatg gccagaaagc cggcgtagag aaagagcagg
       61 atcagctcgg aggtgaggcg tgcgtcccac acccaccacg tgccccaggc gggcttgccc
      121 cagaacgagc cggtccacag cgcgagaaag gtgaacaggg cgccgctcgg cgcgatcgcc
      181 cgggccagca tcgacgccag ccgcgcgttg aaggcccagc cgatcgccgc ccagaacgcc
      241 atcgcgatgt acagcaccat cgacatccag gccgccggca cgtggatgaa gatgatgcga
      301 taggcgtcgc cctgggtggc gtcggtgggt gcccggaaga agccgatgta gaggccggcg
      361 atggcgaaga tcgcgaaggc ggcccagcag atcggcacga gtacgccggc caatcgatag
      421 aagcgcgcgg gcgcggcgaa gtggaacagg cgcaacgtcg tcatgatcgt tgaatcgcag
      481 gcattgtcct gcgttccgca agtccgggga cggtggcggt cattcggtgg cgatgcgaag
      541 cgcggccgat gtcgccggcg gcgcgaccag cgtcgtgaag aggaggaaag cgccgagcag
      601 cgacagctgg ccgcgcgccg acaggccgtc gtcgatcgcc gccaccgcgc cggcgccgaa
      661 gatgagcgcc gggatgcaga gcggcacgat gagcaggatc agcagggcgg cgccgctgcg
      721 caggccgagc gtcagcgccg cgccgaggcc gccgagcagg ctcagcacag gcgtgccgag
      781 cagcaaggtc gccgcgagca tcgcgatcgc ctcgccgcgc atgtcgaaca tgaggccgaa
      841 aagcggcgcg gcgatcacca gaggcaggcc ggtgacgagc cagtgcgcgg tcgccttcgc
      901 gccgacgagc tcgagggcca gcccggcggg ctcggcgagc aggagctgtt cgagggagcc
      961 gtcggcctga tcggcggcgt agagctgcgt gaccgagatc atcgccgcga ggagcgcggc
     1021 gacccagacc acgccggggg cgatctggcg aagcgtttgc gcttcgggcc cgacgccgag
     1081 cggaaagagg ctgatcgcga cggtgaagaa ggcgatcggc agcaaggcgt cggcgggacg
     1141 gcgcgccgcc aggcgcaggt cgcggccgac gacggcagcg aagacgccgc tcatgccggg
     1201 gccggcgcga ccgcgacgag cggcgcttcg agcatcacgg cgaagggccg gggcgagtcg
     1261 atcgaaagcg gcacgtggct ggtcagcacg acgctgccgc cgcgtcgcgc atgcgccgcg
     1321 agcagcgcgt tcagcgattc gatgccggcg gcgtcgaggg cgtcgaaggg ctcgtcgagc
     1381 agccaggccg aggcctcgcg ttcgtgcgcc agccgggcca gcgccacccg gcggcgctgg
     1441 ccctgggaca gcgtcctcac cggggcatcg gcgcggccgg acatggcaaa ggcctcgagg
     1501 gcggtcgaca actcgcccgc ggtcggcctt acgccgccga tgcgcaacag gaaggccagc
     1561 gcctcggcga cgctcaggtc gtccttcagc gcattggcgt gggcaagata gacgacacgg
     1621 cgccggggct gcgcccagac gatgcgaccg gtctcgaccg ggcagaggcc cgcgagggtg
     1681 cgcaacaggg tggtcttgcc ctggccattg gcgccgcgca cccagacgat ctgtccggcg
     1741 cggacgtcga ggtcgacggg acggaacaac tcgcgctcgc cgcgccggca cgcgaggcct
     1801 tgcacggcga gaaccggcat gtcgttgcgg tcgaccggag cggacacgtc tttcctctcg
     1861 ccgcaggccc ggcgaagccg gccgggacga gccccggccg ccgcagattg tgatggctgg
     1921 gcgccggggc cggaggccgc ggaagcgccc gcaacgaggc ggaccgtaaa ctcctcggca
     1981 aacggaggga tgtgcatgtt cttcggcaag ctgctgccgc gcgagggaaa cttcttcg
//
LOCUS       DASRWS010000712         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2586963, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000712 DASRWS010000000
VERSION     DASRWS010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586963"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFF72_13610"
     CDS             complement(<1..351)
                     /locus_tag="VFF72_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZV94251.1"
                     /translation="MSSPFAPGSDRPDPFSSWVGRSLLFIAVVLLLWQGRTLFVPIAI
                     AIVFTFVLAAPVRALRRAGIHEYLGAAIVIAAVLLAIVVIGMLIAAPAAAWWSRAPLI
                     VHELVESVERWRDAL"
     gene            635..1633
                     /locus_tag="VFF72_13615"
     CDS             635..1633
                     /locus_tag="VFF72_13615"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZV94252.1"
                     /translation="MGRLSRHRLSRRPQRGAAAKPRPAPARPLFPELDAALLALLPAR
                     CVLDGEIVVAGPSGLDFDALQQRLHPAATRVARLAKATPASFVAFDLLATRGRSLLDM
                     PQHDRRSALERLLEGKPPPLHLTPATRDRGLATAWLHDFEGAGLDGVVAKPLAAPYRP
                     GERTMLKIKHQRTADCVVAGLRWHKSTLDGGPEAVGSLLLGLFDDAGRLRHVGVTSSF
                     TMAARQELALRLAPLRSRALANHPWRDWNDAAARADPARRLPGAVSRWSKGKDLSWVA
                     LRPERVCEVRYDHLQGDRFRHATTFLRWRPDKPAADCRYDQLEVVEPYALKEIFGR"
     gene            complement(1706..>2321)
                     /locus_tag="VFF72_13620"
     CDS             complement(1706..>2321)
                     /locus_tag="VFF72_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HZV94253.1"
                     /translation="QQAAAGAPACLSGDTPPASASSRDNERGGCIETFGVSFIDPVGI
                     YALSDRATKYGLLFIALTFVAVGAVEVMRRVRVHPIQYLLVGCAIALFFLLLVSLAEH
                     VAFAVAYLAASTACTVLLAFYGCFVLRGVRPGLLFGAGIGALYGVLYVLLQLEQTALL
                     LGSVLLFVVLAALMVATRRIDWYALLERMRRDAVDAAGVATPTA"
BASE COUNT          367 a          846 c          829 g          279 t
ORIGIN      
        1 gagcgcatcg cgccagcgct cgaccgactc gacgagctcg tggacgatga gcggcgcgcg
       61 cgaccaccag gccgcggccg gcgcggcgat cagcatgccg atgacgacga tcgccaagag
      121 caccgcggcg atgacaatcg cagcgcccag gtactcgtgg atgccggcgc ggcgcagcgc
      181 ccgcaccggc gcggcgagca cgaaggtgaa gacgatcgcg atcgcgatcg ggacgaacag
      241 cgtccggccc tgccagagca gcagcacgac ggcgatgaag agcagcgagc ggccgaccca
      301 gctcgagaaa ggatcgggcc ggtcggatcc gggcgcgaat ggcgacgaca cgcccgcatt
      361 gtcgccacgt ggcgacctct cgccggcagc ggccgatcac gccctccctt gccgctgcgg
      421 cgcgggggcc cggtcgggcc ggagcgccga ttgcccgaag cgtgccgatg acgactccat
      481 cgaacgtccg tgtcggcgcg ggcaccgcgc atccggccgg ctccgcaccg gcggcacacg
      541 gtgcgtcgtg gccgccttcg ccgatcgagc cgatgctcgc caaggccgtc gacgcgctgc
      601 ccgtcgacga aggcacgtac ctgttcgagc cgaagtggga cggctttcgc gccatcgtct
      661 ttcgcggcgg ccgcagcgag gtgctgctgc aaagccgcga cctgcgcccg ctcgaccgct
      721 attccccgag ctcgacgcgg cgctgctcgc cctgctgccg gcccgctgcg tcctcgacgg
      781 cgagatcgtc gtcgccggcc cttcgggtct cgacttcgac gccctccagc agcgcctcca
      841 tccggcggcc acacgtgtcg cccggctggc gaaggccacc ccggcgtcgt tcgtcgcctt
      901 cgacctcctc gccacgcgcg gccggtcgct tctcgacatg ccgcagcacg atcggcgaag
      961 cgccctcgag cgcctgctcg aaggcaagcc gccgccgctg cacctgacgc cggcgacccg
     1021 cgaccgcggc ctggccacgg cctggctgca cgacttcgag ggcgcgggcc tcgacggcgt
     1081 cgtcgccaag ccgctggcgg cgccgtaccg gccgggcgaa cggacgatgc tgaagatcaa
     1141 gcaccagcgc accgccgact gcgtcgtcgc cggcctgcgc tggcacaaga gcacgctcga
     1201 cggtggcccc gaggcggtcg gctcgctgct gctcgggctg ttcgacgatg ccggccggct
     1261 gcgccacgtc ggcgtcacgt cgtcgttcac gatggccgcg cggcaggagc tcgcgctacg
     1321 cctcgcgccg ttgcgaagcc gtgccctggc gaaccatccg tggcgagact ggaacgacgc
     1381 cgcggcgcgc gccgacccgg cgcggcgact gccgggcgcc gtgagccgct ggagcaaggg
     1441 caaggacctg tcgtgggtcg cgctgcgtcc ggaacgcgtc tgcgaggtgc gctacgacca
     1501 tctgcagggc gaccggttca ggcacgcgac gacgttcctt cgctggcgcc ccgacaagcc
     1561 ggccgccgac tgtcgctacg accagctcga ggtcgtcgag ccgtatgcgc tgaaggagat
     1621 cttcggccgc tgacgacgcg tcgccgccgg ccccgcggcg atgccgaggc agccgcttcg
     1681 acgacgcatc gccgcgcccc gtggatcacg ccgtgggcgt cgcgacacca gccgcatcga
     1741 ccgcgtcgcg gcgcatccgc tcgagcagcg cataccagtc gatgcgccgc gtcgcgacca
     1801 tcagcgcggc gagaaccacg aagagtagga ccgagccgag gaggagcgcc gtctgctcga
     1861 gctgcagcag cacgtagagc acgccgtaca acgcgccgat gccggcgccg aacagcaacc
     1921 cgggcctgac gccgcgaagg acgaagcagc cgtagaacgc gagcagcacg gtgcaggcgg
     1981 tgctcgcggc gagataggcg acggcgaagg cgacatgctc ggcgaggctc accagcagca
     2041 ggaagaagag cgcgatcgcg caaccgacga gcaggtactg gatcggatgg acgcgcacgc
     2101 gtcgcatgac ctcgacggcg ccgacggcga cgaaggtcag ggcgatgaag agcaggccgt
     2161 atttggtggc ccggtcgctc aatgcgtaga tgccgaccgg gtcgatgaac gagacgccga
     2221 aggtctcgat gcagccaccg cgttcgttgt cgcgggacga tgccgatgcc ggcggcgtat
     2281 cgcccgacag gcaggccggc gcaccggcgg cggcttgctg c
//
LOCUS       DASRWS010000713         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3162261, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000713 DASRWS010000000
VERSION     DASRWS010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162261"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFF72_13625"
     CDS             complement(<1..899)
                     /locus_tag="VFF72_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZV94254.1"
                     /translation="MAEVEGTVSGHRDGHGFVVREGAQPDIFLSTQEMRSVLHRDRVK
                     VRIVRYDKKGRPEGRVLEIVERRKAPIIGRLLHESGIWLVAPEDKRYGQDIIVPRNAI
                     AAATAGQVVAIELTEPPSLHSQPLGRVTEVLGEIDDPGMEIEIAVRKYEVPHVFSDAT
                     LAQAAALPDRIGAADQAGRIDLRDVSLVTIDGEDARDFDDAVYCEPAKIGRSRAANGW
                     RLVVAIADVSHYVVPGTPLDADAYERATSVYFPRRVLPMLPEKLSNGLCSLNPQVERL
                     AMVCDMLVTAEGDIHAYQFFPAV"
     gene            973..1057
                     /locus_tag="VFF72_13630"
     tRNA            973..1057
                     /locus_tag="VFF72_13630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Leu,seq:cag)
     gene            complement(1179..1700)
                     /locus_tag="VFF72_13635"
     CDS             complement(1179..1700)
                     /locus_tag="VFF72_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZV94255.1"
                     /translation="MLTDDGKHLYVSWDEYHMLIERLALKIHVSGWSFDQILCLARGG
                     MRPGDVLSRVFDKPLAIMATSSYRAEAGTIQGRLDLAKYITLPRGELAGRVLLVDDLA
                     DSGITLRAVVDRLRGMPSISELRSAVLWTKGASTYTPDYHVELLPTSPWIHQPFEEYD
                     DLRPDGLAKKFVI"
     gene            complement(1725..>2973)
                     /locus_tag="VFF72_13640"
     CDS             complement(1725..>2973)
                     /locus_tag="VFF72_13640"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZV94256.1"
                     /translation="FQGGHNAGHTLVIRGVKTALQLIPSGVMREGVGCFIGNGVVVDP
                     QHLLGEIARLEAIGLDVRSRLLVSESCPLILPVHVELDKARERLREASGAGKIGTTGK
                     GIGPAYEDKVARRALRVQDLKHPERFAAKLRGLLDLHNFALAGPLHAEPLAFEPMFDA
                     AMRAGEQLRPMLGDVGYALYKLNQAGARILFEGAQGTLLDIDHGTYPYVTSSNCVAGN
                     ASAGSGVGPDQLHYILGITKAYTTRVGAGPFPTELPIDEPGTIGHHLSTVGQERGTVT
                     GRARRCGWLDAALLKRSMIINGISGLCITKLDVLDGLDEIKVCVGYELRGDRLDILPL
                     DADDIDACVPVYESFPGWKETTFGVTRYDALPLNARRYLQRVEAFIGAPVDMISTGPD
                     REHTILLRHPYQPDSRAPRRLAA"
BASE COUNT          480 a          957 c         1032 g          504 t
ORIGIN      
        1 atcaccgccg gaaagaactg ataggcatgg atgtcgccct cggcggtgac aagcatgtcg
       61 cagaccatcg ccaggcgctc gacctgcggg ttgagcgagc acaggccgtt cgagagcttt
      121 tccggaagca tcggcaggac gcggcgcggg aaatacaccg aagtcgcgcg ctcgtacgcg
      181 tcggcgtcga ggggcgtgcc cggcacgacg tagtggctga cgtcggcaat ggcgacgacc
      241 agccgccagc cgttcgcggc gcggcttcga ccgatcttcg ccggctcgca atagacggcg
      301 tcgtcgaaat cccgcgcatc ctcgccgtcg atggtgacca gcgacacgtc gcgcaggtcg
      361 atccgccccg cctggtcggc ggcgccgatc cggtcgggca gcgccgccgc ctgcgccagc
      421 gtcgcgtcgg agaagacgtg cggcacttcg tacttgcgga ccgcgatctc gatttccatg
      481 cccgggtcgt cgatctcgcc gagcacctcg gtgacgcggc ccagcggctg cgaatgcagc
      541 gacggcggtt cggtgagctc gatggcgacc acttggccgg cggtggcggc cgcgatggcg
      601 ttgcgcggaa cgatgatgtc ctgtccgtag cgcttgtcct cgggcgcgac cagccagatg
      661 ccgctctcgt gcagcaggcg gccgatgatc ggcgccttcc tgcgctcgac gatctcgaga
      721 acgcgacctt ccggccggcc cttcttgtcg tagcggacga tgcgtacctt gacgcgatcg
      781 cgatgcagga cggatcgcat ttcctgcgtc gagagaaaga tgtcgggttg cgcgccctcg
      841 cgcacgacga agccatggcc gtcgcggtgg ccggagaccg tgccctcgac ttcggccatc
      901 agctcgttgc cgatgccttg cggggtcttg gatggggttt gtttgatgat aaagtccgtg
      961 gttgcctgag atgcccaggt ggcggaattg gtagacgcac tagtttcagg tactagcggg
     1021 taactccgtg ggggttcgag tcccttcctg ggcaccaagg atgaaaaggg ccgcgccagg
     1081 cggccgccgg gccgcaacga acgcggccac gatgaagagc aagcccgcct cgcgggcttt
     1141 ttttccgcct gctcgccgag cggccgcgct gccccgcatc agatgacgaa cttcttcgcc
     1201 aggccgtccg gccgaaggtc gtcgtattcc tcgaacggct ggtggatcca ggggctcgtc
     1261 ggcagcagtt cgacgtgata gtcaggcgtg taggtcgacg cccccttggt ccagaggacg
     1321 gccgaccgca gctcgctgat cgagggcatg ccgcgcagcc ggtcgaccac ggcgcgaagc
     1381 gtgatgcctg aatcggcaag atcgtccacc agcagcaccc ggccggcaag ctcgccccgg
     1441 ggcagcgtga tgtacttggc caggtcgagc cgcccctgga tcgtcccggc ctcggcgcga
     1501 tacgagctcg tggccatgat cgcgagcggc ttgtcgaaca cgcgcgacag gacgtcgccg
     1561 ggccgcatgc cgccgcgcgc caggcacagg atctggtcga acgaccagcc gctgacatga
     1621 atcttcagcg ccagccgctc gatgagcatg tggtactcgt cccaggagac gtagagatgt
     1681 ttgccgtcgt cggtgagcat gggttgcggc cgggtgatgc gaagtcaggc ggcgaggcgc
     1741 cgcggcgccc tgctgtcggg ctggtaggga tgacgcagca ggatcgtgtg ttctcggtcg
     1801 ggtccggtgg agatcatgtc gaccggagcg ccgatgaagg cctcgacgcg ttgcaggtac
     1861 ctgcgtgcat tgagcggaag cgcgtcgtag cgcgtcacgc cgaacgtcgt ttccttccag
     1921 ccgggaaacg attcgtagac cggtacgcaa gcgtcgatgt cgtcggcgtc gagcggcagg
     1981 atgtcgagcc tgtcgccacg caactcgtag ccgacgcaga ccttgatctc gtcgagaccg
     2041 tcgagcacat cgagcttggt gatgcaaagg cccgagatgc cgttgatgat catcgaacgc
     2101 ttgagcaatg ccgcatcgag ccatccgcac cgccgtgcgc ggcccgtgac cgtgccgcgc
     2161 tcctgcccga cggtcgagag atgatggccg atcgttccgg gctcgtcgat cggcagctcg
     2221 gtcgggaaag gcccggcgcc gacccgcgtc gtgtaggcct tggtgatgcc caggatgtag
     2281 tgcaactgat ccggcccgac acccgatcct gcggacgcgt tgccggccac gcagttgctc
     2341 gacgtgacat acggataggt gccatggtcg atgtcgagca gcgtgccctg cgcgccttcg
     2401 aagaggatgc gcgcgccggc ctgattgagc ttgtagagcg cgtagccgac gtcgccgagc
     2461 atcggccgaa gctgttctcc ggcgcgcatc gccgcgtcga acatcggttc gaatgccagc
     2521 ggctcggcgt gcagcggacc ggccagggcg aagttgtgca ggtcgagcaa gccgcgcagc
     2581 ttggcagcga agcgctcggg gtgcttgaga tcctgcaccc gcagggcccg acgcgcgacc
     2641 ttgtcttcgt aggcggggcc gatccccttg cctgtcgtgc cgatcttgcc ggcaccgctc
     2701 gcctcgcgca gcctttcgcg cgccttgtcg agctcgacgt ggaccggcag gatcagcgga
     2761 cacgactcgc tgaccagcag gcgcgaacgg acgtcgagac cgatcgcctc gagtcttgcg
     2821 atctcgccga gcagatgctg cgggtcgacg acgacgccgt tgccgatgaa gcagccgacg
     2881 ccttcgcgca tgacgcccga cggaatcaac tgcagcgccg tcttgacgcc gcggatgacg
     2941 agcgtatggc cggcgttgtg gccgccctgg aag
//
LOCUS       DASRWS010000714         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3163348, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000714 DASRWS010000000
VERSION     DASRWS010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163348"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..517
                     /locus_tag="VFF72_13645"
     CDS             167..517
                     /locus_tag="VFF72_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94257.1"
                     /translation="MNIIDKTDEEILALAEPLWRGLVTHSNEGKYGEFARNFSSSLAL
                     AMNFVVAGHQFANSELARSLSEDIDPLGIIRRGQHVTVLYRQRSTKKQGEWLGRLVLG
                     YEDAELKIFGASIF"
     gene            538..1047
                     /locus_tag="VFF72_13650"
     CDS             538..1047
                     /locus_tag="VFF72_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94258.1"
                     /translation="MNETIADGKFVELTYRVIDKKSGHVLSRVDFPLGYVHGHNEILA
                     PSVHKELEGKSAGDVIEVPIDGNQIFGPRDDSLVFTSDLESVPEEYRKVGTSILMESD
                     KGQTRSFLVTHMDDDTLTVDGNNPLCGRVVVFTLEILTVRDATDEETMAGEAIVAGAD
                     VDATLMRSV"
     gene            1125..1283
                     /locus_tag="VFF72_13655"
     CDS             1125..1283
                     /locus_tag="VFF72_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94259.1"
                     /translation="MSEQKPVTKVPDGRSRFLTTRQMAPNAKGFVGYETIWKPFQKEV
                     EYKTPKQP"
     gene            1401..2216
                     /locus_tag="VFF72_13660"
     CDS             1401..2216
                     /locus_tag="VFF72_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV94260.1"
                     /translation="MAIDPRALWNFNEPAQSEQRFRSALSGASAEDQFILHTQIARTY
                     GLRKEFTRSQEILRELESTLANASVEAQAWYWLELGRSLSSGTHSSESQTADTKARAR
                     QAYEHALAAARSGGFDGLAIDALHMLAFVDTAPADQLKWGEEALSVALASSQPAAVAW
                     RASLRNNIGYALHQLGRFEEALAQFQEALALREQKSDIQATRIARWMVAWTLRAMERT
                     DEALAIQLRLEAERAAAGTPSSYVFGELELLYLARGDEARAAHYAGLKMATSQ"
     gene            2348..2677
                     /locus_tag="VFF72_13665"
     CDS             2348..2677
                     /locus_tag="VFF72_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94261.1"
                     /translation="MLVCADTHDRSGLVKGYEFDFVVTITDGKLLGQYGAKGRPSSVF
                     YTGEVARDGTLEVNAVGNTGHADRTVGKLARGTEYSYTMTGRLDASHGQAVRRELRPC
                     TATFGRL"
     gene            2702..2953
                     /locus_tag="VFF72_13670"
     CDS             2702..2953
                     /locus_tag="VFF72_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZV94262.1"
                     /translation="MSDTKIVLVLGLVCGIGMLGWALSGVVTGRILTKTYGNDEDGER
                     RYSRHVYRSEEPVWFWVNCATYGIVGIAVLLVVAALWRR"
     gene            complement(3069..4022)
                     /locus_tag="VFF72_13675"
     CDS             complement(3069..4022)
                     /locus_tag="VFF72_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HZV94263.1"
                     /translation="MSAARPLRRTPFTVIGGFLGAGKTTLVNHLLDEAGGAARRLAVL
                     VNDFGAVNIDAASIGARTADTIALSNGCVCCQIGDDLTMALVRVLDAPDPFDAIIVEA
                     SGVSDPWRIAQIARADPALTLEGVVVLVDADAALAQAADPLLADTFERQLRSADLVVL
                     NKIDLARDDDLAATRAWIADVAGKVTIIDATRARVPLALLVGDGRQPGQAWRGTRTLR
                     EAAAAHGIDFESSLFRPQTEFAEGSLRALLAAMPDGVLRLKGLLRTDRWPWAEIQFSG
                     RHGSLREALEVPSDGAAAVVAIGLRGGLPNAELARHFGLPQ"
     gene            complement(4019..>5188)
                     /locus_tag="VFF72_13680"
     CDS             complement(4019..>5188)
                     /locus_tag="VFF72_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZV94264.1"
                     /translation="NSAMLKLAGIDRDTEVEGVVRFAGGARAGEPNGELQEPAAMFLV
                     LRKIGNAGLLGPMTVPGIRSFARLASMQGVTTATDLVNKLSDDDVRVLEQATGDDDCS
                     VRILPAFQAFHGTHGAPDGAEHVKSLVGRNTERLRYGLVKMMLDGSIQGFSSRMRWPG
                     HFNGAPNGIWVTAPAQYEADFETYHRAGLTIHTHTNADEASLVAIAAIERVLARAPRP
                     DHRHTLQHGQMIDAAMFRRMKALGLCANLFANHIWYWGDQHYEMTMGPDRAHRMDACA
                     SALAAGVPLAIHSDAPVTPLGPLHVAWCAVNRVTPKGRLLGESERLTVMQALHAITLG
                     PAWTLKMDHEIGSIECGKRADFCVLDEDPLDVEPMRLKDLRIRGTMLSGRVFEQA"
BASE COUNT          933 a         1709 c         1707 g          839 t
ORIGIN      
        1 gaatacacca tggaaaaggc gccctgctat cacctggtct cagacgagta gagatgtcga
       61 tggacgagct cgcatcgacc gtcttcctgg tgcagcggag gctggcatgc cgtcggccat
      121 gagcccggca gcgcttgccc cggccgggcg cgcggctgag ccgcccgtga acatcatcga
      181 caagaccgat gaagagatcc tggccttggc cgagccgctc tggcgcggcc ttgtcactca
      241 ttcgaacgaa ggcaagtacg gagagttcgc aaggaacttt tccagttccc tggcgctggc
      301 catgaatttc gtcgtcgcag gccaccagtt cgcaaacagt gagctggcga gaagcctgtc
      361 cgaagacatc gacccgctcg gcatcattcg ccgcggccag cacgtgacgg ttctttaccg
      421 gcagcgcagc accaagaagc agggcgaatg gctcggcagg ctggtgctcg gctatgaaga
      481 tgccgagctc aagatcttcg gcgcctccat cttctgagtc gttcatccgg acccgacatg
      541 aatgaaacca tcgcggacgg caagttcgtc gagctcacct acagggtgat cgacaagaag
      601 tcggggcacg tgctcagccg ggtcgacttc cccctgggct acgttcacgg acacaatgaa
      661 atcctggctc cgtcggtcca caaggagctg gagggcaagt ccgccggcga cgtcatcgaa
      721 gtgccgatcg atggcaacca gatcttcggc ccccgggacg actcgctcgt cttcacctcc
      781 gacctcgaga gcgttcccga ggagtaccgg aaagtcggca cctccatcct catggaaagc
      841 gacaagggcc agacccgcag ctttctcgtc acgcacatgg acgacgacac gctgacggtc
      901 gacggcaaca atccgctttg cggcagagtg gtggtcttta cgctggaaat cctgaccgtg
      961 cgtgacgcga cggacgaaga gacaatggcg ggtgaggcga tcgtcgccgg cgcggatgtc
     1021 gacgcaacac tcatgaggtc tgtctaaggc gcggtgtcgg acgacacggc agttccttcg
     1081 tttcctgcac ctgcaatcct gagtcgcgac aagaggggtg aaacatgagc gagcaaaaac
     1141 cggtcaccaa ggtgcccgac ggccgatcgc gctttttgac aacccgccag atggcaccca
     1201 acgccaaggg cttcgtggga tacgaaacca tctggaagcc gttccagaag gaagtcgaat
     1261 acaagacgcc gaagcagccg taggcagcgc aggcgattga ccgggtcgcc actgggagcc
     1321 gaaaaccggg gttgcaagac tgaagtgcat gcgagcacgc gtcctctcac gtccaattcc
     1381 ttcattcctc gccctccgac atggccatcg atccacgcgc cctttggaac ttcaacgagc
     1441 ccgcacagag cgaacagcgc tttcgatccg cgctttcggg cgcctctgcg gaagaccagt
     1501 tcatcctgca tacgcaaatc gcccggacct acggactgcg caaggagttc acccgatcgc
     1561 aggagatcct gcgggagctc gaatcgaccc tggcgaacgc gagcgtcgaa gcgcaggcct
     1621 ggtattggct tgagctgggt cgatcgcttt cgtcaggcac gcactcgtcc gagtcgcaga
     1681 ctgccgacac gaaggcgcgt gccaggcaag cctacgagca cgcacttgcc gcggccagaa
     1741 gtggcggctt cgacggtctg gccatcgacg cgctgcacat gctcgcgttc gtggacacgg
     1801 ccccggccga tcagctcaag tggggagaag aggccctctc ggtcgccctg gcgtcctcgc
     1861 agcccgccgc agtggcgtgg agggcctcgc tgcgcaacaa catcggctat gcactgcatc
     1921 agctcggtcg gttcgaagag gccctggcgc agttccagga ggccctcgcc ttgcgggaac
     1981 agaagagcga catccaggcg acacggatcg cccgctggat ggtcgcctgg accttgcgtg
     2041 cgatggagcg cacggacgag gcgctcgcca tccagcttcg ccttgaagcc gagcgcgctg
     2101 ccgcgggcac gccgagctcc tatgtgttcg gagaactgga attgctgtac ctcgccagag
     2161 gcgatgaagc gcgcgctgcg cattacgccg gtctgaaaat ggcgacgagc cagtgatcgt
     2221 ccgatcaggg ggatttgcaa ccatgagaca actcccggca gtcgcgctcc ttgcgttcct
     2281 ggctgcgtca tgccatgcag cggataccgc cacgacggaa catccttacg atggaaggtg
     2341 gtcggtcgtg ctcgtgtgcg ccgatacgca cgaccgaagc ggcctcgtca agggatacga
     2401 attcgatttc gtcgtcacca tcacggacgg caagctgctg ggtcaatacg gtgccaaggg
     2461 caggccctct tccgtcttct ataccggcga ggtggcccgc gacgggacac tcgaggtgaa
     2521 cgccgtcggc aacaccggcc atgcggatcg aacggtcggc aagctggcgc ggggaaccga
     2581 gtattcgtac acgatgaccg gcaggctcga tgcgtcgcac ggccaggccg tgcgacgcga
     2641 acttcgaccc tgcaccgcca cgttcggaag gctgtagaag ccttgccgcg cggaagcgac
     2701 gatgagtgac acgaagatcg ttctcgttct gggcctcgtc tgcggcatcg gcatgctcgg
     2761 ctgggcgctg tcgggcgtgg ttaccggccg catcctcacc aagacctacg gcaacgacga
     2821 ggatggcgaa cgccgctatt cccgccacgt gtacagaagc gaggaaccgg tctggttctg
     2881 ggtgaactgc gcgacgtacg gaatcgtcgg tatcgccgtg ctgctcgtcg tggccgcctt
     2941 gtggaggcgc tgactcctgt ggcgaaacgg cagtcgcctc gagccgagga cgaccggctg
     3001 gcctgagcga agcttcgatc gagcgcgcag agggcgctcg cctgcgcacg ctgcctcttg
     3061 cgctttcttc attgcggcag cccgaaatgc cgcgccagtt cggcgttcgg tagcccgccg
     3121 cgcagtccga tggcgaccac ggcggcagcg ccatcactcg gcacctcgag cgcttcgcgc
     3181 agcgagccgt gccgacccga gaactggatc tcggcccacg gccagcggtc ggtgcgaaga
     3241 agacccttca ggcgcagcac gccatcgggc atcgccgcca gcaaggcgcg caggctccct
     3301 tccgcaaact ccgtctgcgg ccggaacagc gagctctcga aatcgatgcc gtgcgccgcc
     3361 gctgcctcgc gcaatgtgcg cgtgccgcgc caggcctgtc cgggctggcg cccgtctccg
     3421 accagcagtg ccagcggcac cctcgcccgc gtcgcgtcga tgatcgtgac cttgccggca
     3481 acatcggcga tccaggcccg cgtcgcggcg aggtcgtcgt cccgagcgag atcgatcttg
     3541 ttgagaacga ccagatcggc cgaacgcagt tgccgctcga aggtgtcggc gagcagcggg
     3601 tcggcggcct gcgccagcgc cgcatcggcg tcgacgagga ccaccacccc ttcgagcgtc
     3661 agcgccggat cggcgcgcgc gatctgggcg atccgccacg gatcggagac gccgctggcc
     3721 tcgacgatga tggcatcgaa ggggtcgggc gcgtcgagca cgcgcaccaa cgccatcgtc
     3781 aggtcgtcgc cgatctggca gcagacgcag ccgttgctga gggcaatcgt gtcagcggtc
     3841 cgcgcaccga tcgacgcggc atcgatgttg accgcgccga agtcgttgac gaggacggcc
     3901 aggcgtcgcg cggcgccgcc cgcctcgtca agcaagtggt tgaccagggt tgtctttccg
     3961 gcgccgagaa atccgccgat caccgtgaac ggcgttcgac gaagcggcct cgcggcgctc
     4021 acgcctgctc gaagacgcgc cccgacaaca tcgtgccgcg aatgcgcaga tccttcaggc
     4081 gcatcggctc gacgtcgagc ggatcttcgt cgagaacaca gaagtcggcg cgcttgccgc
     4141 actcgatgga accgatctcg tggtccatct tcagcgtcca ggccggcccg agcgtgatcg
     4201 cgtgcagggc ctgcatgacc gtgagacgtt cgctctcgcc cagaaggcgg cccttgggcg
     4261 tgacgcgatt gacggcgcac caggccacat gcagcggacc gaggggggtc accggcgcgt
     4321 cggaatggat cgccagcggc acgccggcgg ccagcgccga ggcacaggca tccatgcgat
     4381 gggcgcgatc cgggcccatc gtcatctcgt agtgctggtc gccccagtac cagatgtggt
     4441 tggcgaagag attggcgcac aacccgagcg ccttcatgcg gcgaaacatc gccgcatcga
     4501 tcatctggcc atgctgcagc gtgtggcgat ggtcgggccg gggcgcccgc gccagcaccc
     4561 gctcgatcgc ggcgatggcg acgaggctcg cctcgtcggc gttggtgtgg gtgtggatcg
     4621 tcaggccggc gcggtggtag gtctcgaagt cggcctcgta ctgcgccggc gccgtcaccc
     4681 agatgccgtt gggcgcgccg ttgaagtggc ccggccagcg catgcgcgac gaaaaaccct
     4741 ggatcgagcc gtcgagcatc atcttgacga ggccgtagcg cagccgctcg gtgttgcgcc
     4801 cgaccaggct cttcacatgc tcggcgccgt cgggcgcgcc gtgcgtgccg tggaaagcct
     4861 ggaaggccgg caggatgcgc acgctgcagt cgtcgtcgcc ggtggcctgc tcgagcacgc
     4921 gcacgtcgtc gtcgctcagc ttgttgacca ggtcggtggc cgtcgtcacg ccctgcatcg
     4981 acgcgagccg cgcgaacgag cggatgcccg gcacggtcat cggcccgagc agcccggcgt
     5041 tgccgatctt gcgcagcacc aggaacatcg ccgccggctc ctgcagctcg ccgttgggct
     5101 cgccggcgcg ggcgccgccc gcgaagcgga ccacgccctc gacctcggtg tcgcggtcga
     5161 tgccggccag cttgagcatc gccgagtt
//
LOCUS       DASRWS010000715         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2009313, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000715 DASRWS010000000
VERSION     DASRWS010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009313"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFF72_13685"
     CDS             <1..1323
                     /locus_tag="VFF72_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZV94265.1"
                     /translation="HMSVLEGLTKINMDGKVTPLLAESWSTDPDGKVYTFKLRKGVKF
                     HDGEAFDASDVKFSFERAKAPGSTNKAKKAVFDNIARIDTPDASTVIVVLNNADGNFL
                     FRMGENTAVILDPKSAPTTATKPIGTGPYKFDAWQKGSSITLVKNDQFRDAAKVQMKK
                     VTFRFINDPAAQVAALLAGDIDGMPRFGALESLKQFQNDPRFSVTVGGTEGKTIMTIN
                     NKKKPFDDVRVRRALAAAIDRKAIIDGAQEGYGKPIGSHMVPSDAGYVDLTGVNPYNP
                     EKAKALLKEAGVQLPLNVTLTLPPPAYARKGGEIIAAELAKVGIIAKIENVEWAQWLS
                     GTFKGNFDLTVISHVEPLDFDRYGDPHYYYHYDSPAYRKLLDEYNTTTDTKARLKVLG
                     DIQRKLADDCVNVYLFQLPQFAVGNKHLKGMWSSSPIFANDPAALRWQ"
     gene            1349..2764
                     /locus_tag="VFF72_13690"
     CDS             1349..2764
                     /locus_tag="VFF72_13690"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZV94266.1"
                     /translation="MTTPLHDLAATELVERYRRRDLSPVDATRAVLARIDAWEPHLHA
                     TYALDGDAALAAARASEARWQKGAPLGPIDGVPSMIKENIATRGTPTPLGTAATLLAP
                     AASDAPPAARMRESGAVILGKTTMPDYGMLSSGLSSFHATARNPWDLSKGPGGSSAGA
                     GAGVAAGYGPLHVGTDIGGSVRLPAGWCGIFTLKPSLGRIPIDPPYAGRVAGPMTRSV
                     ADAALLMQVLAQPDERDTMSLPAQAIDWSRLDRDVRGLRLGLVTGAAGLPVDPEIAAA
                     VRAAAERFAAAGAEVDEVAPFLSRAMLDGMDRFWRMRSWLDYSALPSERQARVLPFIV
                     DWLKGGEGLSGEEVFRGYSQMAAMRDAAITAHRPFDFVLSPTSPIPAFRAELASPTDD
                     PMRPLEHIGFTVAYNMSEQPAASINCGWTGSGLPIGLQIAGKRHDDLGVLQVARAWET
                     MRPAQRAWPEPPARARAAAAG"
     gene            2781..>3551
                     /locus_tag="VFF72_13695"
     CDS             2781..>3551
                     /locus_tag="VFF72_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZV94267.1"
                     /translation="MTLRDAHGNAVGTASHEALAAAEQALWRMMSFYGTPVGDLDCAI
                     AADPGWLLPRVMKAGFLLGLTEPDLVADGRAALGEAEARSGSGNARERGHLAAVQRVA
                     LGDWQGASDAWQEVLDAHPRDALALQWVLLFDFYRGDTQALLERVARASPEWHEDDPL
                     FAYVLGLHAFGLEETGHYAEAEAAGRRALAGAAKVPWAIHAVAHVMEMQGRHDEGRRW
                     MAQWRPDWGEGNGFAGHLGWHEAVFCLESLDAEAALVPF"
BASE COUNT          568 a         1270 c         1236 g          477 t
ORIGIN      
        1 cacatgagcg tcctcgaggg cctgaccaag atcaacatgg acggcaaggt gacgccgctg
       61 ctcgccgaga gctggtcgac cgaccccgac ggcaaggtct acacgttcaa gctgaggaag
      121 ggcgtcaagt tccacgatgg cgaggcgttc gacgcgagcg acgtcaagtt cagcttcgag
      181 cgcgccaagg cgccggggtc gacgaacaag gcgaagaagg cggtgttcga caacatcgcg
      241 cggatcgaca cacccgatgc gtcgacggtg atcgtcgtcc tcaacaacgc cgacggcaat
      301 ttcctgttcc gcatgggcga gaacacggcg gtgatcctcg atccgaaaag cgcgccgacg
      361 acggcgacca agccgatcgg caccgggccg tacaagttcg atgcgtggca gaaggggtcg
      421 tcgatcacgc tggtcaagaa cgaccagttc cgcgacgccg ccaaggtgca gatgaagaag
      481 gtgacgtttc gcttcatcaa cgacccggcg gcgcaggtcg cggcgctgct cgccggcgac
      541 atcgacggca tgccgcgctt cggcgcgctc gagtcgctca agcagttcca gaacgatccg
      601 cgcttctcgg tgaccgtcgg cggcaccgag ggcaagacga tcatgacgat caacaacaag
      661 aagaaaccct tcgacgacgt ccgcgtccgc cgcgcgctcg ccgcggcgat cgaccgcaag
      721 gcgatcatcg acggcgcgca ggagggctac ggcaagccga tcggcagcca catggtgccg
      781 agcgacgccg gctacgtcga cctcaccggc gtcaacccgt acaacccgga aaaggccaag
      841 gcgctgctga aggaagcggg cgtgcagttg ccgctcaacg tcacgctgac gctgccgccg
      901 ccggcctacg cccgcaaggg cggcgagatc atcgccgccg agctggccaa ggtcggcatc
      961 atcgccaaga tcgagaacgt cgaatgggca cagtggctct cgggcacgtt caagggcaac
     1021 ttcgacctga ccgtgatcag ccacgtcgag ccgctcgatt tcgaccgcta cggcgatccc
     1081 cactactact accactacga ctcgccggcc taccgcaagc tcctcgacga atacaacacg
     1141 acgaccgaca cgaaggcgcg cctgaaggtg ctgggcgaca tccagcgcaa gctcgccgac
     1201 gattgcgtca acgtctatct gttccagctg ccgcagttcg cggtcggcaa caagcacctg
     1261 aagggcatgt ggagcagctc gccgatcttc gccaacgatc cggcggcgtt gcgctggcag
     1321 tgacgaccgc gagccggcgc cgcgcgccat gacgacgccg ctgcacgacc tggcggcgac
     1381 cgagctggtc gagcgctatc gccggcgcga cctctcgccg gtcgacgcga cgcgcgccgt
     1441 gctggcgcgc atcgacgcct gggagccgca cctgcatgcg acctacgcgc tcgacggcga
     1501 cgccgcgctg gccgccgcgc gcgcctccga ggcacgctgg caaaaggggg cgccgctcgg
     1561 gccgatcgac ggcgtgccgt cgatgatcaa ggaaaacatc gccacgcgcg gcacgccgac
     1621 accgctcggc accgccgcga ccctcctcgc gccggccgcg agcgacgcgc cgccggcggc
     1681 gcggatgcgc gagtcgggcg ccgtcatcct cggcaagacg acgatgcccg actacgggat
     1741 gctgtcgtcg ggcctgtcga gctttcacgc gacggcgcga aacccgtggg acctgagcaa
     1801 ggggccgggc ggcagcagcg ccggcgccgg cgccggtgtc gccgccggct acgggccgct
     1861 gcacgtcggc accgacatcg gcggctcggt ccgcctgccc gccggctggt gcgggatctt
     1921 cacgctgaag cccagcctcg gccgcatccc gatcgacccg ccgtatgccg gccgcgtcgc
     1981 cgggccgatg acgcgcagcg tcgccgacgc ggcgctgctg atgcaggtgc tggcgcagcc
     2041 cgacgaacgc gacacgatga gcctgccggc gcaggcgatc gactggtcgc ggctcgatcg
     2101 cgacgtgcgc gggctgcggc tcggcctcgt gacgggagcg gccggcctgc ccgtcgatcc
     2161 cgagatcgcc gccgccgtgc gcgccgccgc cgaacgcttc gccgctgccg gcgccgaggt
     2221 cgacgaggtc gcgccgtttc tgagccgcgc catgctcgac ggcatggacc ggttctggcg
     2281 gatgcgctcg tggctcgact actcggcgct gccgtccgag cgccaggcca gggtgctgcc
     2341 cttcatcgtc gactggctga agggcggcga agggctcagc ggcgaagagg tgtttcgcgg
     2401 ctacagccag atggcggcga tgcgcgacgc cgcgatcacg gcgcaccgtc cattcgattt
     2461 cgtcctgtcg ccgacatcgc cgatcccggc ctttcgcgcc gagctggctt cgccgaccga
     2521 cgatccgatg cgtccgctcg aacacatcgg cttcacggtc gcctacaaca tgagcgagca
     2581 gcctgccgcc agcatcaatt gcggctggac cggcagcggg ctgccgatcg gcctgcagat
     2641 cgccggcaag cgccacgacg acctcggcgt gctccaggtg gcacgcgcct gggagacgat
     2701 gcggccggcg cagcgcgcct ggcccgagcc gccggcgcgg gccagggccg ccgccgcggg
     2761 ctgacggcgt cgccgcagcg atgaccctgc gcgacgcgca cggcaacgcc gtcggcaccg
     2821 cgtcgcacga ggcgttggcc gccgccgagc aggcgctctg gcggatgatg tcgttctacg
     2881 gcacgccggt gggtgatctc gattgcgcca tcgccgccga tcccggctgg ctgttgccgc
     2941 gcgtcatgaa ggccggcttc ctgctcggcc tgaccgagcc cgatctcgtc gccgatggcc
     3001 gcgcggcgct cggggaggcc gaggcacgct ccggctcggg caacgcccgc gagcgcggcc
     3061 atctcgcggc ggtgcaacgc gtcgcactcg gcgactggca aggcgccagc gacgcctggc
     3121 aggaggtgct cgacgcccat ccgcgcgacg cgctggccct gcagtgggtg cttctcttcg
     3181 acttctatcg gggcgacacg caggcgttgc tcgagagggt ggcacgcgcg tcgcccgagt
     3241 ggcacgaaga cgatccgctc ttcgcctacg tcctcggcct gcacgctttc ggcctcgagg
     3301 aaacggggca ctacgccgag gccgaggcgg ccggtcgccg cgccctcgcc ggcgcggcga
     3361 aggtgccgtg ggcgatccac gccgtcgccc acgtcatgga gatgcagggc cggcacgacg
     3421 agggccgccg ctggatggcg cagtggcggc ccgactgggg cgagggcaac ggctttgccg
     3481 gccatctcgg ctggcacgag gccgtgttct gcctcgagtc gctcgacgcc gaggccgcgc
     3541 tggtgccctt c
//
LOCUS       DASRWS010000716         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_3164411, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000716 DASRWS010000000
VERSION     DASRWS010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3164411"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFF72_13700"
     CDS             <1..411
                     /locus_tag="VFF72_13700"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZV94268.1"
                     /translation="AGQQGSRVATLPEVRANLLRFQKEFSSQASGAVLDGRDIGTVIC
                     PDAPVKLFVTASADSRAERRYRELRERGVDTIKPRVLAEMAERDRRDSERAAAPLKAA
                     PDAWLLDTSDMDADAAFAAALAFIERKGFRSSGP"
     gene            564..>2228
                     /gene="rpsA"
                     /locus_tag="VFF72_13705"
     CDS             564..>2228
                     /gene="rpsA"
                     /locus_tag="VFF72_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZV94269.1"
                     /translation="MAKGSALRKPANDAASAEFAALLDESLGGSSSFEGTVVKGTIVR
                     IANDFVVVDVGLKSEGRVALREFANGGAAPEVKEGDVVDIFVDRMENKEGEAVLSRDK
                     ARREEAWTLLEKAFNDQERVMGTIFGRVKGGFTVDLSGAVAFLPGSQVDVRPVRDVGP
                     LMGQAQQFAILKMDRRRGNIVVSRRAVMEETRAEDRTRLMGALSEGQVLDGVVKNITD
                     YGAFVDLGGVDGLLHVTDIAWKRINHPSEALTIGQQVKVQVIRFNPETQRISLGMKQL
                     MSDPWDGAGAKYPVGLKLKGRVTNITDYGAFVELEPGVEGLVHVSEMSWTKKNVHPGK
                     IVSTSQEVEVMVLDVDMSKRRISLGLKQCMANPWESFAEKYPSSTELEGEVRNITEFG
                     LFVGLPGDIDGMVHLSDLSWDKAGDEAIRDYKKSDAVKVKVLDVDIDKERISLGIKQL
                     ANDPFEKVGSVAKKGDVVTVIVAGIQDNGIDVTVQDGIPGFIRKSELSRDRSEQRPDR
                     YAIGDKLDAKITNIDKASRRVVLSVKARELDEEKKAMADFGSSDSGA"
BASE COUNT          424 a          684 c          756 g          364 t
ORIGIN      
        1 gcgggccagc agggatcgag ggtcgccaca ctcccggagg tccgggccaa cctcttgaga
       61 ttccaaaagg aattctcatc tcaggcatct ggcgccgtgc tcgacgggcg ggatatcgga
      121 actgtgatct gtcctgatgc accggtgaag ctcttcgtca cggcgagcgc tgactcgcgc
      181 gccgaacgcc gataccggga gttgcgcgag cgcggggtcg acactataaa accgcgcgtt
      241 cttgccgaga tggcggagcg ggaccgtcgc gatagcgaac gggcggcagc acctctgaaa
      301 gccgcgcccg atgcctggct tctcgatacc agcgacatgg atgccgatgc ggcttttgcc
      361 gcggcattgg cgttcatcga gcgcaagggc tttcggtctt ccgggccata aatcgccgcc
      421 ggcagatcga agacttcaag cggcgattgg cggacggacg gattgtccgg acgccggagt
      481 ctcaaggcag tggatccgcc ggacttaacc ggttggccga cgggcggcag cgtttcgccc
      541 gggatcgaga aggagagttc tgaatggcta agggcagcgc cctgcgtaaa cccgcgaacg
      601 atgccgcaag tgcggagttc gccgcactcc ttgatgaatc gctcggcggt tcgtcgagtt
      661 tcgaaggtac ggtggtcaag gggaccatcg tgcgcatcgc caacgatttc gtcgtcgtcg
      721 atgtcggcct caagtccgaa ggccgtgtcg ccctgcgcga gttcgccaat ggcggcgccg
      781 cacccgaggt gaaggaaggc gacgtggtcg acatcttcgt cgaccgcatg gagaacaagg
      841 agggcgaggc cgtcctgtcg cgcgacaagg cgcggcgcga ggaagcctgg actttgctcg
      901 agaaggcgtt caacgaccag gaacgcgtca tgggcaccat cttcggccgt gtgaagggcg
      961 gcttcaccgt cgacctcagc ggtgccgtgg cgttcctgcc gggcagccag gtcgatgtgc
     1021 gtccggtgcg cgacgtcggc ccgctgatgg gccaggcgca gcagttcgcg atcctcaaga
     1081 tggatcgccg gcgcggcaac atcgtcgtgt cgcgccgtgc cgtgatggag gagacccggg
     1141 ccgaggaccg cacccgcctg atgggggcgc tgtcggaggg ccaggtgctc gacggcgtgg
     1201 tcaagaacat caccgattac ggcgcattcg tggacctggg tggcgtcgac ggcctgctgc
     1261 acgtcaccga catcgcctgg aagcgcatca accatccgtc ggaagccctg accatcggcc
     1321 agcaggtcaa ggtgcaggtc atccgcttca atcccgagac gcaacgcatc tcgctcggca
     1381 tgaagcagct catgagcgat ccgtgggacg gcgcaggggc caagtacccg gtcgggctca
     1441 agctcaaggg ccgcgtcacc aacatcaccg actacggcgc gttcgtcgag ctggagccgg
     1501 gtgtcgaggg cctggtccat gtctccgaga tgagctggac caagaagaac gtgcaccctg
     1561 gcaagatcgt gtcgacctcg caggaggtcg aagtgatggt gctggacgtc gacatgtcca
     1621 agcgccgcat ctcgctcggc ctcaagcagt gcatggccaa tccatgggag agcttcgccg
     1681 agaagtatcc gtcgagcacc gagctcgagg gcgaagtccg caacatcacc gagttcggcc
     1741 tgttcgtcgg cctgcccggc gacatcgacg gcatggtgca tctctccgac ctgtcgtggg
     1801 acaaggccgg cgacgaggcg atccgcgact acaagaagag cgatgccgtc aaggtgaagg
     1861 tgctcgacgt cgacatcgac aaggagcgca tctcgctcgg catcaagcag ctcgccaacg
     1921 atccgttcga gaaggtgggc tcggtcgcca agaagggcga cgtggtcacc gtcatcgtgg
     1981 ccggcatcca ggacaacggc atcgacgtca cggtgcagga cggcatcccg ggcttcatcc
     2041 gcaagtcgga gctctcccgc gaccgctccg agcagcgtcc tgatcgttac gcgatcggcg
     2101 acaagctcga cgccaagatc accaacatcg acaaggcctc gcgccgggtc gtgctgtcgg
     2161 tcaaggcgcg cgagctcgac gaagagaaga aggcgatggc cgatttcggc tcgtccgatt
     2221 cgggcgcc
//
LOCUS       DASRWS010000717         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Caldimonas sp. isolate SMAG_U11227 k141_2011027, whole
            genome shotgun sequence.
ACCESSION   DASRWS010000717 DASRWS010000000
VERSION     DASRWS010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970238
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldimonas sp. (soil metagenome)
  ORGANISM  Caldimonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Caldimonas.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:38:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,743
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Caldimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011027"
                     /isolate="SMAG_U11227"
                     /isolation_source="soil"
                     /db_xref="taxon:2838790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..987)
                     /locus_tag="VFF72_13710"
     CDS             complement(184..987)
                     /locus_tag="VFF72_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HZV94270.1"
                     /translation="MLIAQLSDTHLRSDGELLYDRIDTAAYLERAVAHVLKLDPRPDV
                     VIMTGDLVEAGKPAEYARLRRLIAPLPMPVYVIPGNHDAREALRTAFADQGYFPASGF
                     LQYTVDDLPIRLIALDTLVEGKGYGALCQERLAWLEARLAEQTDRPAMLFMHHPPFEC
                     GIDAFDHARLQEGDDRLAELVRKSGNVERAVCGHVHRPIQVRWAGTMASIAPSTAHQA
                     TLDLHDGAPLSMMMEPPAIALHQWRPNTGLVTHVSYVGEFDGPKPFRKL"
     gene            complement(990..2645)
                     /locus_tag="VFF72_13715"
     CDS             complement(990..2645)
                     /locus_tag="VFF72_13715"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZV94271.1"
                     /translation="MSDSLKVALAQLNPKVGDVAGNLAKVRAARAEAKEQGADLVLTA
                     ELVLAGYFPEDLVLKPSFVKRCHEAVEALRADTADGGPGVFVATPWHEDGKLYNAIVA
                     LHKGCVIGKRFKVDLPNYGVFDDKRVFSPGPMPGPLVFDGVRVGVPICEDVWTADVVE
                     CVAETGGEILLVPNASPYEVGKTDYRVQLGAKRVIESGLPFAYLNQVGGQDEVIYDGG
                     SFVLGADRMLKAKLASFREQVATVEFRRQAGGWDCLPGPIAPELEQIDSIYQAMMLGL
                     RDYVNKTGFPSVIIGLSGGVDSALTAAVAVDALGADRVHTVMMPSPYTSKESLEDAKA
                     CAEAIGVKYDIVGIRPAMEAFRAMLLPLFGNRKEDVTEENIQSRARGVTLMALSNKLG
                     GMVLTTGNKSEVAVGYATLYGDMCGGYNVLKDVYKTTVFELCKWRNAHRPEGALGRTG
                     PVIPERIITKPPSAELKPDQKDEDSLPPYPVLDTILKGLIERDLSAEELTKEGHDAAT
                     VQRVWRLLEGAEYKRRQAPPGVKITSRLISRERRYPIVNGFRG"
BASE COUNT          454 a          939 c          942 g          461 t
ORIGIN      
        1 ggacaacgcc gagtgcaggc gcgaggttgc tcaagttccg tcgtcccgac cgaagccgag
       61 cgaagcgagg cgtagcggag ggaccttttt tcagcgactt gcggctgatc gcggaaaaag
      121 gtccctccgc tacgcgcctt cggcgcttcg gtcgggacga cggaaaatag aaatcactcc
      181 gctctacagc ttgcggaagg gcttgggccc gtcgaactcg ccgacgtagg acacgtgcgt
      241 gaccaggccg gtgttcggcc gccattgatg cagggcgatg gccggcggct ccatcatcat
      301 tgagagtggc gcgccgtcat gcagatcgag cgtcgcctgg tgcgccgtgc tgggcgcgat
      361 cgaggccatg gttccggccc aacgcacctg gatcgggcga tggacgtggc cacacacggc
      421 acgctcgacg ttgccgctct tgcggaccag ttcggccagc cggtcgtcgc cctcctgcag
      481 gcgcgcatga tcgaaggcgt cgatgccgca ttcgaacggc ggatggtgca tgaacagcat
      541 cgccggcctg tcggtctgct cggccaaccg cgcctccagc cacgccagcc gctcctggca
      601 cagcgcgcca taacctttgc cttcgaccag cgtgtcgagc gcgatcaggc ggatcggcag
      661 gtcgtcgacc gtgtactgca ggaaacccga cgccgggaag tagccctggt cggcaaacgc
      721 cgtccgcagg gcctcgcgcg catcgtggtt gccggggatg acatagaccg gcatcggcag
      781 cggcgcgatc aggcggcgca ggcgcgcgta ctcggcgggc ttgcccgcct cgacgaggtc
      841 gccggtcatg atgaccacgt cgggtcgcgg atcgagcttc agcacatgcg ccacggcgcg
      901 ctcgagatag gccgctgtat cgatgcggtc gtagagaagc tcgccgtcgg accgaagatg
      961 ggtgtcggaa agctgcgcga tcagcatgat cagcccctga agccgttgac gatcggatag
     1021 cgccgctcgc gcgagatcag gcgcgaggtg atcttcacgc cgggcggcgc ctgccgccgc
     1081 ttgtactccg ccccttccag aagacgccag acgcgctgga cggtcgcggc gtcgtggcct
     1141 tccttcgtga gctcctccgc cgacaggtcg cgctcgatca ggcccttgag gatggtgtcg
     1201 agcaccgggt agggcggcag cgaatcctcg tccttctggt cgggcttgag ctcggccgac
     1261 ggcggcttgg tgatgatgcg ctcgggaatg acgggccctg tgcggcccag cgcgccctcg
     1321 ggtcggtgcg cattgcgcca cttgcagagc tcgaacaccg tcgtcttgta gacgtccttc
     1381 agcacattgt agccaccgca catgtcgcca tagagcgtgg cgtagccgac cgcgacctcc
     1441 gacttgttgc cggtggtcag caccatgccg ccgagcttgt tcgagagggc catcagggtg
     1501 acgccgcggg cgcggctctg gatgttctcc tcggtgacgt cttccttgcg gttgccgaac
     1561 aagggcagga gcatcgcgcg gaaagcttcc atcgcgggcc ggatgccgac gatgtcgtac
     1621 ttcacgccga tcgcctcggc gcaggctttc gcatcctcca gcgactcctt gctggtgtag
     1681 ggcgacggca tcatgaccgt gtgcacgcgg tccgcgccca gcgcgtcgac ggcgacggct
     1741 gcggtcagcg ccgaatcgac gccacccgac aggccgatga tcaccgatgg gaagccggtc
     1801 ttgttcacat agtcgcgcag gccgagcatc atcgcctggt agatcgaatc gatctgctcg
     1861 agttccggcg caatcgggcc cggcaggcag tcccagcctc ccgcctggcg gcggaactcg
     1921 acggtcgcca cctgctcgcg gaacgaggcg agcttggcct tgagcatgcg gtcggcgccc
     1981 agcacgaacg agccaccgtc gtagatcacc tcatcctggc cgccgacctg gttgagatag
     2041 gcgaagggca gaccgctttc gatcacccgc ttggcgccga gctgcactcg atagtcggtc
     2101 ttgccgacct cgtagggcga ggcattgggc accagcagga tctcgccgcc ggtctcggcg
     2161 acgcattcca cgacgtcggc cgtccagacg tcctcgcaga tcggcacgcc gacgcgcacg
     2221 ccgtcgaaca ccagcgggcc cggcatcggc ccgggcgaga agacgcgctt gtcgtcgaac
     2281 acaccgtaat tcggcaggtc gaccttgaag cgcttgccga tgacacagcc tttgtgcagg
     2341 gcgacgatgg cgttgtagag cttgccgtcc tcgtgccacg gcgtggcgac gaacacgccc
     2401 ggcccgccgt cggcggtgtc ggcgcgcagg gcctcgaccg cctcgtggca gcgcttcacg
     2461 aaggacggct tcagcaccag gtcctcgggg aagtaacccg cgaggaccag ctccgccgtc
     2521 agcaccaggt ccgcgccctg ctccttcgcc tcggcgcgcg ccgccctgac cttcgccagg
     2581 ttgccggcga cgtcgccgac cttgggattg agctgggcga gcgcgacctt gaggctgtcc
     2641 gacattaccg tcgtcctacc cgtcgtcccg agcggagcgc gaagcgcgaa gtcgagggac
     2701 cttttctcca cgacaagccg caaaacagcg gagagaaggt ccctccactg cgcttcgctg
     2761 aagcgcgggg atgccccgcg cgagtcggga cgacgg
//
